LOCUS       DASUVE010000001         3298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_376875, whole genome shotgun sequence.
ACCESSION   DASUVE010000001 DASUVE010000000
VERSION     DASUVE010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376875"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VF036_00005"
     CDS             <1..482
                     /locus_tag="VF036_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX6263641.1"
                     /translation="GDQAYFDIIHVFNGYRTCYYRTFVVGRATQAQRDAFARSREWMD
                     AAIDLVKPGVSTDQIAAVWPKAEEFGFSDEMDAFGLQFGHGVGVSLHERPIITRLNSF
                     VDPLEIKEGMLFALETYCPAADGRSAARIEEEVIVTADGCRVITLFPCDELMVANEY"
     gene            497..1519
                     /locus_tag="VF036_00010"
     CDS             497..1519
                     /locus_tag="VF036_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005505224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX6263642.1"
                     /translation="MENTREAERKDVSGVANLETRLSIYRMMQEGRLFEERARDLFLQ
                     GLVKGTSHLGIGQEAVAAGFAAALEPDDLVFCTYRGHVHTLLRGAPMAALMAELLGRA
                     TGICGGKGGSMHLTDVSHGAMGSYAIVGAHMPIAAGAAWAAKARGTGQVTVCFFGDGT
                     TNIGTFHEALNLAAVWELPVVFVCENNLYMEYTPIGDVTAVEHPAADRASAYGLEPIL
                     IDGNDADVVHATAVRTVARARSGEGPSLVEAVTYRHGGHSRADPGKYRPDEEVAEWMR
                     RDPIPSYRERLLAAGADEGALDAIDRDALAAVDRATEEAREAPFHDPDTLETELWADG
                     GSAWRN"
     gene            1507..2481
                     /locus_tag="VF036_00015"
     CDS             1507..2481
                     /locus_tag="VF036_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEX6263643.1"
                     /translation="MAELTYREAVARGIAQEMERDPSVFFIGEDVAAAGGVFKTTEGL
                     FDRFGPERVRDTPISEQAIIGAAMGAAMNGLRPIAELMFSDFFAVCWDMVANQIAKAR
                     YMTNGQVALPIVIRTANGGGARFGAQHSQSVETWMMAIPGLKVVVPSTPLDMIGLLAA
                     AVRDPDPVVVVEPKVLYASKGDVPDGEIVDELGIARTVREGGDVTLCALGAMIPKALE
                     AAERLSDDGIDADVLDVRSLVPLDVRAIADSVERTGHLVTVEENPRLCGWGAEVASIV
                     ADEQFYALDGPIVRVTTPHLPLPSADVLEDAAIPSVDRIVDGVRRSLG"
     gene            2492..>3298
                     /locus_tag="VF036_00020"
     CDS             2492..>3298
                     /locus_tag="VF036_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003996217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6263644.1"
                     /translation="MTSRHLVKKGNELELTAPPGSWVGDLRRWSVVDEHVPGAVHTGF
                     AIVELAGSGRIPWHVHSYEDSVYVLEGELVIDTGEAANLLRAGDYGVTPVGVPHALRN
                     VSETPVRWAAMWAPQPRSRVQGDTYAVPGVPDREPTPIDVRDPRTRSFGHIDPANMEV
                     DKQTQDMLAVSASMRTALLVYSGITVKMMVDADLGAQLLTMFMVQYEPRGLAGRHDHP
                     YEETYLILDGEVDAEFDGGRYHLVPGDIAWAGVGCVHAFTNPGPGIVRWLE"
BASE COUNT          514 a         1097 c         1199 g          488 t
ORIGIN      
        1 ccggcgatca ggcgtacttc gacatcatcc acgttttcaa cggctaccgg acgtgctact
       61 accgcacgtt cgtcgtcggc cgggcgacgc aggcgcagcg cgacgcgttc gcccggtcgc
      121 gcgagtggat ggatgccgcg atcgacctgg tcaagcccgg cgtctccacc gaccagatcg
      181 cggcggtgtg gccgaaggcc gaggagttcg ggttctcaga cgagatggac gcgttcggat
      241 tgcagttcgg ccacggggtc ggcgtgagcc tgcacgagcg gccgatcatc acgaggctga
      301 actcgttcgt ggatcccctc gagatcaagg aagggatgct cttcgcgctc gagacctact
      361 gccccgcggc ggacggccga tcggccgcgc gcatcgagga ggaggtcatc gtcaccgccg
      421 acggctgccg cgtgatcacg ctgttcccgt gcgacgagct catggtcgcg aacgagtact
      481 gaggggtcgg acgttgatgg agaacacgcg agaggccgag aggaaggacg tctcgggggt
      541 cgcgaacctc gagaccaggc tctcgatcta ccggatgatg caggagggcc gcctcttcga
      601 ggagcgtgcc agggatctgt tcctccaggg gctggtgaaa ggcacctccc acctcgggat
      661 aggccaggaa gcggtcgccg ccggcttcgc ggcggcgctc gagccggatg atctcgtgtt
      721 ctgcacgtac cgcgggcacg tccacacgtt gctgcgaggg gcgccgatgg ccgcgctgat
      781 ggcggaactc ctcgggcgcg ccacaggcat ctgcggcggc aagggcggtt cgatgcacct
      841 caccgacgtc tcgcacggcg cgatgggctc ctacgccatc gtcggggcgc acatgccgat
      901 cgccgccggc gccgcgtggg cggccaaggc ccgggggacg ggccaggtga ccgtgtgctt
      961 cttcggtgac gggacgacga acatcggcac gttccacgaa gcgctcaacc tggccgcggt
     1021 ctgggagctc cccgtcgtgt tcgtctgcga gaacaacctc tacatggagt acacgccgat
     1081 cggcgacgtg accgcggtgg agcacccggc cgccgatcgc gcatcggcct acggcctgga
     1141 gccgatcctg atcgatggga acgacgccga cgtcgtgcac gcgacggcgg tgcgcaccgt
     1201 cgcccgggcc cgctccggcg aaggcccgtc actcgtcgag gccgtgacgt atcgccacgg
     1261 cgggcactcg cgcgccgacc cggggaagta ccgtcccgac gaggaggtcg cggagtggat
     1321 gcgccgcgac ccgatcccgt cctaccgcga gcggctcctc gcggcgggag ccgacgaggg
     1381 ggcgctcgac gccatcgacc gcgacgcgct cgctgcggtg gaccgcgcca ccgaggaggc
     1441 gcgcgaggcg ccgttccacg atccggacac gctcgagacc gagctctggg ccgacggagg
     1501 ctccgcgtgg cggaactgac ctaccgcgag gcggtcgccc gagggatcgc tcaggagatg
     1561 gagcgcgatc cctcggtctt cttcatcggg gaggacgtcg cggccgccgg gggcgtcttc
     1621 aagacgacgg agggactctt cgatcgcttc gggcccgagc gggtccggga cacgccgatc
     1681 agcgagcagg cgatcatcgg cgcagcgatg ggggcggcga tgaacggcct ccgcccgatc
     1741 gccgagctga tgttcagcga cttcttcgcc gtgtgctggg acatggtggc gaaccagatc
     1801 gcgaaggccc ggtacatgac gaacgggcag gtggcgctgc cgatcgtgat ccgcaccgcg
     1861 aacggcggcg gcgcgcgatt cggcgcccag cactcccaga gcgtcgagac gtggatgatg
     1921 gcgatccccg ggttgaaggt cgtcgtcccg tccacgccgc tcgacatgat cggactcctc
     1981 gcggccgcgg tgcgcgaccc cgacccggtc gtggtggtgg aaccgaaggt gctgtacgca
     2041 tcgaaaggcg acgtgcccga cggtgagatc gtcgacgagc tcggtatcgc acgcaccgtc
     2101 cgagagggtg gggacgtcac gctctgcgcg ctcggcgcga tgatccccaa ggcgctcgag
     2161 gctgcggagc ggctctccga cgacggcatc gatgccgacg tgctcgatgt ccgctccctg
     2221 gtcccgctcg acgtccgcgc gatcgcggac tcggtggagc ggaccggtca cctcgtcacg
     2281 gtcgaggaga acccgcggct gtgcggctgg ggggcagagg tcgcgtcgat cgtggccgac
     2341 gagcagttct atgccctcga cggtccgatc gtccgggtca cgaccccgca tctgccgttg
     2401 ccgtccgccg acgtcctgga ggacgccgcg atcccgtcgg tcgatcggat cgtcgacggt
     2461 gttcggcgct cgctgggatg aggagcgcct catgacctcc cgccacctcg tcaagaaggg
     2521 gaacgagctc gagctcacgg ctccgccagg atcgtgggta ggggatctcc gacggtggtc
     2581 cgtcgtcgac gagcacgttc ccggtgccgt acacaccggg ttcgcgatcg tcgagctcgc
     2641 gggcagcggg cgcatcccct ggcacgtcca cagctacgag gacagtgtct acgtgctcga
     2701 gggggagctc gtgatcgaca ccggcgaggc agcgaacctg cttcgggccg gcgactatgg
     2761 ggtgacgccg gtcggggtac cgcacgcgct ccgcaacgtc tccgagacgc ccgtgcgatg
     2821 ggcggcgatg tgggcaccgc agccgcggtc gcgcgtgcag ggcgacacct atgcggttcc
     2881 cggggtgccg gaccgcgagc ccacccccat cgacgtgcgc gatccgcgca cgcgctcgtt
     2941 cggacacatc gacccggcga acatggaggt cgacaagcag acgcaggaca tgctggcggt
     3001 atcggcgagc atgcggacgg cgctgctcgt ctacagcggc atcaccgtga agatgatggt
     3061 ggacgcggac ctcggcgcgc agctgctcac gatgttcatg gtccagtacg agccccgcgg
     3121 gctcgccggc cgccacgacc atccgtacga ggagacgtac ctcatcctcg acggggaggt
     3181 cgacgcggag ttcgacgggg ggcgctacca cctcgtcccg ggcgacatcg cgtgggccgg
     3241 cgtgggctgc gtgcatgcgt tcaccaaccc gggaccgggg atcgtgcgat ggctcgag
//
LOCUS       DASUVE010000002         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1507225, whole genome shotgun sequence.
ACCESSION   DASUVE010000002 DASUVE010000000
VERSION     DASUVE010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507225"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..542)
                     /locus_tag="VF036_00025"
     CDS             complement(72..542)
                     /locus_tag="VF036_00025"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX6263645.1"
                     /translation="MDASTIWKFLHIASMFVVVSIFVGQGMLSGAVARSGDVRAVRRV
                     LAAEDRFAPIGGGLFLLGIVFGFITAITSDFDLKETWLLIGYGLSLFILVNGLTYQRM
                     QAEKLKAAAAGGSEDQPSEELRALAGAPSGVVMNVIDGLAWLAIIYVMVAKPFA"
     gene            605..1114
                     /locus_tag="VF036_00030"
     CDS             605..1114
                     /locus_tag="VF036_00030"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6263646.1"
                     /translation="MSGSLLEDAFGHHVWATLLVLDACLALPPEQLETSVPGTYGSIL
                     DTARHTVGADASYLFVLSGGRVTPIDEDSMDLAELRSTMEAFGAAWSETLTNDFDPDV
                     MLVRHRDDGSETHAPASIRLAQALHHGTDHRSQIATALTTLGVEPPAMDVWDYGNEVG
                     RVTEIPPTS"
     gene            1111..1914
                     /locus_tag="VF036_00035"
     CDS             1111..1914
                     /locus_tag="VF036_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX6263647.1"
                     /translation="MTSDRQASKAAAFRALHDGEPFVIPNPWDAGSARVLEALGFRAL
                     ATTSSGLAFTLGRLDGSVTLNEMAEHAGALDRATELPVSADLENGYGAEPEQAATAIR
                     RAAEAGAVGGSIEDFDRDERRLYPLDHATARIVAAVEAARELPFPFTLTARAENHIWG
                     NPDTHDTIRRLQAFEEAGADVLYAPGLETIDDIRTVCGSVAKPVNVLARPAMRLAELA
                     DAGARRVSVGGALTWAAVKAFADAATAIRDVGDLSVLDADVPLDEWFGG"
     gene            complement(1928..>2361)
                     /locus_tag="VF036_00040"
     CDS             complement(1928..>2361)
                     /locus_tag="VF036_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018854150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6263648.1"
                     /translation="IGTFLLGRRMYEVLVAWETMDTTDEPSTIEDYAHIWRAADKIVY
                     SRSLDAVTSAKTRLERSFDADAVREMKASAERDISVGGPDLAAQAIRAGLVDEIQLFL
                     SPVVVGGGTRALPDGVRLDLELLEERRFGNGTVLVRYRSGT"
BASE COUNT          369 a          825 c          797 g          370 t
ORIGIN      
        1 acccgctgtt cgtgatcatg gatcggctct tcgacgccgc gcaccggctg ggctgaccgg
       61 ggccgccgga gtcacgcgaa cggcttcgcc accatcacgt agatgatcgc gagccacgcg
      121 aggccgtcga tcacgttcat cacgacgccg ctcggtgcgc cggccagcgc ccggagctcc
      181 tcggacggct gatcctccga gccccctgcc gcggctgcct tgagcttctc cgcctgcatg
      241 cgctgatagg tcagaccgtt cacgaggatg aagagcgaga gtccgtaccc gatcaggagc
      301 cacgtctcct tgaggtcgaa gtcgctcgtg atcgcggtga tgaacccgaa cacgatgccg
      361 agcaggaaca gacctccgcc gatgggcgcg aaccggtcct cggccgccag gacccgtcgg
      421 acggcacgga cgtctcccga gcgtgcgacg gcaccggaca gcatcccctg accgacgaag
      481 atcgagacca ccacgaacat cgacgcgatg tgtaggaact tccagatcgt cgacgcgtcc
      541 atcccggtcc tccgatgctc gcacccggac gttggcgtct caaaccctct gttacgttgg
      601 gcgcatgagc ggttcgttgc tggaagacgc gttcgggcac cacgtgtggg cgacgctgct
      661 cgtcctcgat gcctgcctgg cgctcccgcc ggagcagctg gagacctcgg tccccgggac
      721 gtacggctcg atcctcgata cggcgcgtca cacggtcggt gcggatgcgt cgtacctgtt
      781 cgtcctgagc ggtggtcgcg tcacgccgat cgatgaggac tcgatggacc tcgccgagct
      841 ccgctccacg atggaagcgt tcggagccgc ttggtcggag accctcacga acgacttcga
      901 cccggacgtg atgctcgtgc gccatcgcga cgacggttcc gagacccacg ctcccgcgag
      961 tatccggttg gcccaggcct tgcaccacgg caccgaccat cgcagtcaga tcgcgaccgc
     1021 ccttacgacg ctcggggtgg agccgccggc gatggacgtc tgggactacg gcaacgaggt
     1081 gggtcgggtc accgagatcc cgcccacgtc gtgacatcgg atcgacaggc gtcgaaagcc
     1141 gccgcgttcc gcgcgttgca cgacggcgag cccttcgtga tcccgaatcc atgggacgcc
     1201 ggatcggctc gcgtgctcga ggccctcggg ttccgggcgc tcgcgacgac gagctccgga
     1261 ctcgccttca ctctgggtcg tctcgacggg agcgtgacgc tcaacgagat ggcggagcac
     1321 gcgggggcac tcgatcgcgc gaccgagctc cccgtctcgg cggacctcga gaacggctac
     1381 ggcgccgaac cggagcaggc cgccaccgcg atccgtcgag ccgcggaggc gggcgccgtc
     1441 ggcgggtcga tcgaggactt cgaccgcgac gagcgccgtc tgtatcccct ggaccacgcg
     1501 accgcgcgga tcgtcgcggc ggtggaggcg gctcgcgagc tcccgttccc gttcacgctc
     1561 acggcgcggg ccgagaacca catctggggg aaccccgaca cgcatgacac gatccgccgg
     1621 ctgcaggcgt tcgaggaggc cggcgccgac gtcctctacg cccccggcct cgagaccatc
     1681 gatgacatcc ggacggtctg cggatcggtg gcgaagccgg tgaacgtgct ggcccggccg
     1741 gcgatgcgcc tcgccgagct cgccgacgcg ggtgcgcgcc gcgtgagcgt cggcggtgcg
     1801 ctcacgtggg cggccgtgaa ggcgttcgcc gacgcagcga ccgccatccg tgacgtcgga
     1861 gacctctcgg tcctggacgc cgacgtgccg ctggacgagt ggttcggggg atgagccgcg
     1921 atcctgttca agttccgctg cggtaacgca cgagcacggt gccgttgccg aagcggcgct
     1981 cctccaacag ttcgaggtcg aggcgcacgc cgtcggggag ggctcgggtc ccacctccga
     2041 ccacgaccgg tgagaggaac agctggatct catcgacgag tcccgcccgg atcgcttgtg
     2101 ccgcgagatc gggaccgccc acggagatgt cccgctccgc ggaggccttc atctcgcgga
     2161 cggcgtcggc gtcgaagctc cgttcgagcc gcgtcttcgc actggtcacg gcgtcgagcg
     2221 acctcgagta cacgatcttg tctgccgcac gccagatgtg ggcgtagtcc tcgatcgtgg
     2281 acggctcgtc cgtggtgtcc atcgtttccc aggccaccag cacctcgtac atgcgtctcc
     2341 cgaggaggaa agtgccgatc g
//
LOCUS       DASUVE010000003         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_942152, whole genome shotgun sequence.
ACCESSION   DASUVE010000003 DASUVE010000000
VERSION     DASUVE010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942152"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..866)
                     /gene="tatC"
                     /locus_tag="VF036_00045"
     CDS             complement(84..866)
                     /gene="tatC"
                     /locus_tag="VF036_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX6263649.1"
                     /translation="MRTLPRWRRKTPEQREGAMSLIDHLKELRHRLLICLWALLGASV
                     AGFFLYEPAMRVLQSPYCDFLRENPAKAPFQTEECSLIFLGAIDGFLVTVKVIIFLAL
                     AIALPVILYQLWAFIVPGLTDRERRWAVPFVALSLILFLLGGLAAYLTLPKALDFLLG
                     VGGDFVSPLLTVDKYVGFVIFTILAFGLGFEFPILLISLSAAGVLTKETMRRYRRQVV
                     LGLAIFAAVITPSPDPISMLALLIPLYLFYEGAIIVSRLLKR"
     gene            complement(863..1345)
                     /locus_tag="VF036_00050"
     CDS             complement(863..1345)
                     /locus_tag="VF036_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6263650.1"
                     /translation="MLNVGPLELVVVLAVALIVVGPERLPELARSVGRVLRQFREVQD
                     EVRDMVSSGVDDDMRQAADEFRQATGGIKRATDVKGAVRRAERTIRDETAAPRTSRTP
                     RSKRVGRSSAPAEPTEASGPPADRLVEDAGTTSASGDASGDTHASEGTPPDPESTPDP
                     "
     gene            complement(1374..1622)
                     /locus_tag="VF036_00055"
     CDS             complement(1374..1622)
                     /locus_tag="VF036_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263651.1"
                     /translation="MEAWLVVWLVVGLVTTVALLVMLGFLVRHVILLGRTAGRMQEEL
                     QPIADELAAASARAADTASRLSERASPQGASARRRGPR"
     gene            complement(1678..2055)
                     /locus_tag="VF036_00060"
     CDS             complement(1678..2055)
                     /locus_tag="VF036_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263652.1"
                     /translation="MTTIRTTCPRCGEVDMGPEAISLSVRSSGREGSYRFTCPRCDDA
                     VEKRADRKIVALLVSAGVDLEPRGSAEMSESLFDQEPMEAVEPLEHAMDGPAFSIDDV
                     IEFHFLLEDDHYLKEFLAGSEES"
     gene            complement(2129..>2537)
                     /locus_tag="VF036_00065"
     CDS             complement(2129..>2537)
                     /locus_tag="VF036_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX6263653.1"
                     /translation="GMGHWYVAAWDVDADAERLFRADRVRSATPTGETFVPRGLRGAG
                     RDLYSPTGDDVSVRLRLRPAARWIAEYYATTELEELNDGGVEATLPARQLGWVARLLV
                     RVGDDAEVVEPAELRDEVRRLARETLAGYGHKT"
BASE COUNT          405 a          857 c          902 g          373 t
ORIGIN      
        1 ggggacgggt ccgcgcctcg gggcgcgcac gggctgtctc gccctcgtcc gtcctttgcc
       61 cttgatcgcc atgatccggt ctcctatcgc ttcagcaggc ggctgacgat gatagctccc
      121 tcgtagaaca ggtagagggg gatcaacaat gcgagcatcg agatcgggtc ggggctcggt
      181 gtgatcacgg ccgcgaagat ggcgagcccc aggaccacct gtcgccggta gcgccgcatc
      241 gtctctttcg tgaggacgcc cgcggccgac agggagatca gcaggatcgg gaactcgaat
      301 ccgagcccga acgcgaggat cgtgaagatc acgaacccga cgtacttgtc gaccgtgagc
      361 aacgggctca cgaagtcgcc ccccacgccg agcaggaagt cgagcgcttt cggcagcgtc
      421 aggtaggcgg ccagtccgcc gagcaggaac aggatcagcg acaacgccac gaacgggacg
      481 gcccaacggc gctcgcggtc cgtgagcccg ggcacgatga acgcccacag ctggtacagg
      541 atcacgggga gtgcgatcgc gagggcaagg aagatgatca ctttgactgt gacgaggaac
      601 ccgtcgatcg cgccgaggaa gatgagcgag cactcctccg tctggaacgg ggccttcgcc
      661 gggttctcgc ggaggaagtc gcagtacggg ctctggagca cgcgcatcgc gggctcgtag
      721 aggaagaagc ccgcgaccga agcaccgagc agagcccaca gacagatcag gagtcggtga
      781 cggagctcct tgagatggtc gatgagcgac atcgcgccct cgcgctgctc gggggtcttc
      841 cggcgccacc tcgggagcgt cctcatgggt cgggtgtcga ctcgggatcg ggtggtgtgc
      901 cctctgaggc gtgcgtgtca ccggaggcgt cgccggacgc ggacgtggtc ccggcgtcct
      961 ccacgagccg gtcggcgggt ggtccagacg cttcggtcgg ctcggccggg gccgaggaac
     1021 gaccgacacg cttcgagcgt ggggtgcggg acgtccgggg cgcggcggtc tcgtcccgga
     1081 tcgtccgttc ggcccggcgg acggcgccct tcacgtcggt cgcccgcttg atgccgccgg
     1141 tggcctgacg gaactcgtcc gccgcctggc gcatgtcgtc gtcgaccccc gacgacacca
     1201 tgtcgcggac ctcgtcctgt acctcccgga actggcggag cacgcggccg acggaacggg
     1261 cgagctcggg caggcgttcc ggccccacca cgatcagcgc cacggcgagg acgacgacga
     1321 gctcgagggg tcctacgttg agcatcggtc tcgggacgtg cccggcagcg agtctatcgg
     1381 ggacccctgc ggcgggcgga tgccccctga ggggacgcgc gctccgagag gcgggatgcc
     1441 gtgtcggcgg cccgggccga ggcggcggcc agctcgtcgg cgatcggctg cagctcctcc
     1501 tgcatccgcc cggcggtccg cccgagcagg atcacgtggc gaacgaggaa ccccagcatc
     1561 acgagcaggg cgacggtggt cacgagaccg accacgagcc agacaacgag ccaggcttcc
     1621 acgggaggga tggtaccggg cggacgtgcg ggaggatgcc tgccggcgag gcgggagcta
     1681 cgactcttcc gagccggcca ggaactcctt gaggtagtgg tcgtcctcga ggaggaagtg
     1741 gaactcgatc acatcgtcga tcgagaaggc gggaccgtcc atcgcgtgtt cgagcggctc
     1801 gacggcctcc atgggctcct gatcgaagag gctctcgctc atctccgcgc tccctctcgg
     1861 ttcgagatcg acgccggcgg acacgagcag ggcaacgatc ttgcgatcgg cccgcttctc
     1921 gacggcgtcg tcgcaacggg ggcacgtgaa gcggtagctc ccctcccggc cgctggaacg
     1981 gaccgagagc gagatcgctt cggggcccat gtcgacctct ccgcagcggg ggcatgtcgt
     2041 ccggatggtg gtcatctcac gacagctatc ggccctccgg cgacgcggct tgaggctcgc
     2101 gagcgttgtc ggggctagac ccgtctgact aggtcttgtg gccgtagccc gcgagggtct
     2161 cgcgggcgag ccgtcgcacc tcgtctctga gctcggcggg ctcgacgacc tcggcgtcgt
     2221 ccccgacgcg aaccagcagc cgcgcgaccc agccgagctg ccgtgccgga agcgtcgcct
     2281 cgactccccc gtcgttgagt tcctcgagct cggtcgtggc gtagtactcg gcgatccagc
     2341 gcgccgccgg tcgcagtcgc aggcgcacgg atacgtcgtc tcccgtcggc gaatacaggt
     2401 cccgccccgc tccgcgcagg ccgcgcggca cgaacgtctc gccggtcggc gttgcgctcc
     2461 ggacccgatc cgcgcggaag agacgctctg catccgcgtc cacgtcccag gccgccacgt
     2521 accaatgccc catcccc
//
LOCUS       DASUVE010000004         6376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1319394, whole genome shotgun sequence.
ACCESSION   DASUVE010000004 DASUVE010000000
VERSION     DASUVE010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319394"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VF036_00070"
     CDS             complement(<1..648)
                     /locus_tag="VF036_00070"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX6263654.1"
                     /translation="MSLRDVLDALVSAAAIERLLLSRERPIVAKVDAGQDALLAALAV
                     ALDAPLMVVTPGPREAEDLVPEVGAYLGPDRVALLPAWDALPYEGMDPAPEVAARRAD
                     AVGRLRGASGPFVVVAPFLAAMQAIPPTLGTARTVQLAAGIELAPDALAERLSELGYD
                     RVDVVEHRGEFAVRGGVVDVFPGIARRPARLEYWGDEIERLREFSPSTQLSTKQLG"
     gene            complement(722..2863)
                     /locus_tag="VF036_00075"
     CDS             complement(722..2863)
                     /locus_tag="VF036_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6263655.1"
                     /translation="MSRSSDRRRSDGAKGDLREGRRPLLRFYELLVTLPFLAFLAFEM
                     YHDPSAFTGWSNLLPILVWIAAIMVVDLMPVPMAMSFDFSLSFPLELSVALLYPPPVA
                     ALIVLLGSADVREFRRQIPPLTAMFVRAQIALAVLAESQVFRGLTDLDDGWFVVGPTV
                     LLAAIVGYTVNTILVAEWQAIKSRATLDAILRQMHSGVLGEFVVSYMGLALFSVLVAI
                     TTQTIGLWAIAVFIAPLAFAWQMLHRTHSLKIASEELAEKQRENEYQAFHDHLTGLPN
                     RLLFRLQLGDAIEEAAANGGRLAVMLMDLDHFKEVNDALGHHVGDRLLAAVGPRLAET
                     IREEDLMARLGGDEFGVLVRDVADDETAIGIADRLIEGLHHSVTVDELDLDVSASIGI
                     SFYPDHASDADTLLRHADVAMYASKETGTTFEVYDESIDTHKPELVKLVSQVRPAIDD
                     EQFRMYFQPKIRLSDGRVAGAEALIRWHHPTLGRLSPAAFIPMVEKTVLLQPLTHWAL
                     NDVLRVWRRWSDEGIKIPVAVNVSPRTLLDQDLPQVVGDLLARWGVPPRYLRLELTEN
                     FLVADSGRSDAVLNGLSQVGVGLSIDDFGTGFSSLSYLKRLPIEEIKIDRSFVSHMLE
                     RVEDFTIVRATVELGRNLGLRVVAEGVQDRDTFDRLGDFGCDEAQGFYIARPLEPEDF
                     WRWLSHREMSGEALDPSLADVPSLHGSLRAV"
     gene            complement(2850..3416)
                     /locus_tag="VF036_00080"
     CDS             complement(2850..3416)
                     /locus_tag="VF036_00080"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HEX6263656.1"
                     /translation="MKLVAIGLVLGVVVGTFRGGRLSQLSELKPRYAALAFAGLLLQS
                     VNPPGSWPLVLLILSFVLLTAFALANIRIVGFAAILAGVVLNFAVIAINGGMPVAREA
                     IIASGQEGTLAPLLEHPGAKHHLAEPDDRMLFLGDVIAVPAPVSQVISVGDLFTYGGM
                     AMVIAGAMRRRPSPSRLSPLAEAPRVQV"
     gene            complement(3927..4508)
                     /gene="pth"
                     /locus_tag="VF036_00085"
     CDS             complement(3927..4508)
                     /gene="pth"
                     /locus_tag="VF036_00085"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004006136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX6263657.1"
                     /translation="MAWLVAGLGNPDDRYSRTRHNLGRMVVDELAREEGERFRKVRFL
                     PVESAEIRVADGRIILARSTRYMNESGPSYASLAKKQGIEPAYVIAVHDELDIPSGTI
                     RIKQGGGNSGHNGLRSLDQALRTPDYLRVRVGIGRPPGRQDPSDYVLQPVGAREETDL
                     ALLVSHSADAVRTLITDGLERTQDRFNRSTPSS"
     gene            complement(4512..5258)
                     /locus_tag="VF036_00090"
     CDS             complement(4512..5258)
                     /locus_tag="VF036_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX6263658.1"
                     /translation="MEAILQAEPRSETGKGPAHRLRASGRVPAVLYGAGVDPTPIHVS
                     ALDLLHVFHAMGQASVLVDLKPNGSEYLTIAREIQRDLIHNRFIHIDFMAVNRDEKIT
                     VSVELHEVGDSEGVHAGGVIEHHLREVEIESLPANVPQRVEFDISKLKIGDSVRIGDL
                     VPPEGVEFLTDPDTMILAVVEPAVMDTELDLAVAGEEAPDEAAEAAEEAEGEEAAEGE
                     EAPEGEGAEGTEGTGAEGGGEASTEGDGES"
     gene            5332..>6376
                     /locus_tag="VF036_00095"
     CDS             5332..>6376
                     /locus_tag="VF036_00095"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6263659.1"
                     /translation="MPPARAVGALLVLAVLAATCQDPPRARDTPDLLPPGRPERTDAS
                     AFDTRWPIKHVVFVIKENRTTDHLFGRFPGVNGVTEGYDGDDLRPLTPPPDRLPDDIP
                     HCYECGLESWNEGRMNRFARNDISDRFAYTQMQGPEHLPNYWRWAEEFALSDNFFSSA
                     QGPSFPNHLYTIAAQSGGSHHNPFQDQARATLFTQETGLAKSWGCDTLEEVYVEVVDS
                     EGQVERVPPCFDFPTEADLLDGAGIPWGYYAAWNDQRGYVWSAFSAIERVRMNPAIWQ
                     RHIFPVDNFVADVDDGRLAPVTWVTPRYELSEHPEWSFCHGENWTTEIVNALMESEFW
                     RDAAIFITWDDYGG"
BASE COUNT         1065 a         2164 c         2183 g          964 t
ORIGIN      
        1 gccgagctgc ttcgtcgaga gctgcgtcga cggggagaac tcccggagcc gctcgatctc
       61 gtcgccccag tactcgagcc gcgccgggcg ccgggcgatg cccgggaaca cgtcgacgac
      121 cccgccacgg acggcgaact cgccacggtg ctccaccacg tccacgcggt cgtagccgag
      181 ctccgagagt cgctccgcga gggcgtcggg cgcgagctcg atgccggccg cgagctgcac
      241 cgtccgtgcc gtgccgaggg tcggagggat cgcctgcatc gcggccagga agggagctac
      301 gacgacgaag ggtcccgacg ctccgcggag gcgcccgacc gcgtcggccc tgcgggccgc
      361 cacctccggc gccggatcca tcccctcgta cggcagcgcg tcccaagccg gcaggagcgc
      421 cacgcggtcc ggaccgaggt acgcgccgac ctcggggacc aggtcctctg cctcgcgagg
      481 gccgggggtg acgaccatga gcggggcgtc cagcgcgacc gccagcgccg ccaggagcgc
      541 gtcctgtccg gcgtcgacct tcgcgacgat cggtcgctct cgcgaaagca ggaggcgctc
      601 gatggccgcg gcactcacca gcgcatcgag cacatcccgg agcgacatcc cacctcccga
      661 aaacgcgaac acacaacgcg aacagccccg gtcgcatcgc gacggggcat cgagagggat
      721 tctagacggc gcggagcgat ccgtggaggg acggcacgtc cgcgagcgac ggatcgaggg
      781 cctcgccgga catctcccga tgcgacagcc agcgccagaa gtcttccggc tccaacggcc
      841 gggcgatgta gaagccctgc gcttcgtcgc acccgaagtc accgagccga tcgaacgtgt
      901 cgcgatcctg gacgccttca gcgaccaccc gcagcccgag gttcctcccg agctctaccg
      961 ttgcccggac gatcgtgaag tcctcgacgc gctccagcat gtgggacacg aaggaccggt
     1021 cgatcttgat ctcctcgatc ggcaaccgct tcaggtagga gagcgaggag aaacccgtcc
     1081 cgaagtcatc gatcgaaagg cccacgccca cctgtgagag gccgttcagg accgcgtcgg
     1141 agcgaccgga gtcggccacg aggaagttct cggtcagctc gagacggagg tacctgggcg
     1201 gcacgcccca gcgggccagg aggtccccga cgacctgagg gaggtcctga tcgagcaggg
     1261 tcctcgggga gacgttgacg gccaccggga tcttgatccc ctcgtcggac caccggcgcc
     1321 agacgcgcag gacatcgttc agcgcccaat gcgtgagcgg ctggagcagc accgtcttct
     1381 ccaccatcgg gatgaaggcg gcgggcgaga gacgtccgag ggttgggtgg tgccagcgga
     1441 tcagcgcctc cgcgcccgcg acccggccgt cggagagccg gatcttcggc tggaagtaca
     1501 tccggaactg ctcgtcatcg atcgcggggc ggacctggct cacgagcttg accagctcgg
     1561 gcttgtgcgt gtcgatcgac tcgtcgtaga cctcgaacgt cgtcccggtc tccttcgacg
     1621 cgtacatcgc gacgtcggcg tggcggagca acgtgtcagc gtcggacgcg tggtccgggt
     1681 agaacgagat gccgatgctc gccgagacgt ccaggtcgag ctcgtcgacc gtcaccgagt
     1741 gatggagccc ttcgatcaag cggtcggcga tccctatcgc ggtttcatcg tccgcgacgt
     1801 ctcggaccaa gacgccgaac tcgtcgccgc cgaggcgcgc catcaggtct tcctctctga
     1861 tggtctcggc gagtctcggt ccgacggcgg cgagaagccg gtcgcccacg tggtggccga
     1921 gggcgtcgtt gacctccttg aagtgatcga gatccatcag catcaccgcg aggcggcccc
     1981 cgttcgcggc cgcttcctcg atcgcatccc cgagctgcag gcggaacagg agccggttcg
     2041 ggagcccggt cagatgatcg tggaacgcct ggtactcgtt ctcgcgctgc ttctcggcga
     2101 gctcctcgga cgcgatcttc agggagtgcg tgcggtggag catctgccat gcgaaggcga
     2161 gcggggcgat gaacacggcg atcgcccaca agccgatcgt ctgcgtggtg atcgcgacga
     2221 ggacgctgaa cagggccaga cccatgtacg agaccacgaa ctcgccgagc acgccgctgt
     2281 gcatctgacg aaggatcgcg tccagcgtcg cgcggctctt gatcgcctgc cattcggcga
     2341 cgaggatcgt gttgaccgta tacccgacga tcgctgcgag gaggaccgtc ggacccacga
     2401 cgaaccaacc gtcatcgagg tctgtgagtc cccggaacac ctgactctcc gcgagcaccg
     2461 ccagggcgat ctgggcacgc acgaacatcg cggtgagcgg agggatctgt ctccggaact
     2521 cacggacgtc cgcggagccg agcagcacga tcagagcggc gacgggcggc ggatagagca
     2581 gcgccaccga cagctccagc gggaagctca ggctgaagtc gaacgacatc gccatcggca
     2641 ccggcatcag gtccacgacc atgatcgccg cgatccacac caggatcggc agcaggttcg
     2701 accatccggt gaacgccgag gggtcgtggt acatctcgaa cgcgaggaag gcgaggaacg
     2761 gcaacgtgac caacagctcg tagaaccgga ggagcggtcg ccgtccctcg cggaggtcgc
     2821 ccttcgcgcc atcgctccgg cgacgatcgc tagacctgga cacggggagc ctccgcgagc
     2881 ggtgacagtc gagaaggcga tgggcgccgc cgcatcgctc cggcgatcac catcgccatg
     2941 ccaccgtagg tgaacaggtc gccgacgctg atcacctggc tcacgggtgc aggcaccgcg
     3001 atcacgtcgc cgaggaacaa catccgatcg tcgggctcgg ccaggtggtg tttcgcccca
     3061 gggtgctcga gcagcggagc gagagtcccc tcctggccgg aggcgatgat cgcctcacga
     3121 gcgaccggca tcccgccgtt gatcgcgatc accgcgaagt tcaggacgac gccggcgagg
     3181 atcgccgcga atccgacgat cctgatgttc gcgagcgcga acgccgtcag gaggacgaag
     3241 gacaggatca agagcacgag cggccaggag cccggcgggt tcaccgattg gagcaacagt
     3301 ccggcgaacg cgagggcagc gtaccgcggc ttgagctcgg acagctgcga cagtcgaccg
     3361 ccgcggaagg tgccgacgac gacgcccagc acgagaccga tcgccaccag cttcatggtc
     3421 taggtatcgt ccccttcccg gacggctgaa gcgccccgac gcggaagctc ccggcgtgtc
     3481 cggggtcgga cacgaccggg agccgatgca gcttgaacta cctcagaacg gacgccagcc
     3541 ggcacccacc gccatcgcga gggcggccag ccaagcgagc gcgtacgcga tcttgcggag
     3601 tcgcatccgt ctctccttcg ttggtcggac ctgcgtcagc agggcttcca gcgagcgtcg
     3661 acggagacct ggggaaccgt aacggtgcgc atctcgtcga cctccttgtc catcgaggtc
     3721 gaggatgacc ggcgcacggg ggtcgggcca tcgggcgacg gtcccgagtt tccatcggcc
     3781 gggcaccccc gaccttcgtc gggggtcggc cgccatcgcg ggtgacgcgg ataggccgat
     3841 ggcccaaccc ccatcccacc gaaggggagg gcaggtagtc ctcccgaacc ggtgtcagcg
     3901 gcttggaggc cggtttcccc gtcggctcag gaggaagggg tggaacggtt gaatcgatcc
     3961 tgggtgcgct cgagcccgtc cgtgatcagc gttcggacgg catcggcgga gtggctcacg
     4021 agcagcgcga ggtccgtctc ctcccgagcc ccgaccggct ggagcacgta gtcggatggg
     4081 tcctgccgtc cgggcgggcg gccgatcccc acgcgcacgc gcaggtagtc gggggtgcgg
     4141 agggcctggt cgagcgaacg gaggccgttg tgaccgctgt tgccaccccc ttgcttgatc
     4201 cggatcgtgc ccgaggggat gtccagctcg tcatggacgg cgatcacgta ggcgggttcg
     4261 atcccttgct tcttggccag cgaggcgtag gagggccccg actcgttcat gtatcgcgtg
     4321 gatcgcgcga ggatgatccg cccgtccgcc acacggatct cggcggactc gacggggagg
     4381 aaccgcacct tccggaagcg ctcgccttcc tcgcgagcca actcgtcgac gaccatgcgg
     4441 ccgaggttgt ggcgcgtccg cgagtagcga tcgtccgggt tgccgaggcc ggcgacgagc
     4501 caggccatcg gctagctctc gccgtccccc tcggtggagg cttcgccgcc gccctcggcc
     4561 ccggtccctt cggtgccctc ggcgccctcg ccctcgggag cctcttcgcc ctcggcggcc
     4621 tcctcgccct ccgcctcctc ggcggcctcg gctgcctcgt ccggggcctc ttcgcccgcg
     4681 accgcgaggt cgagctccgt gtccatgacc gcgggctcca cgacggcgag gatcatcgtg
     4741 tcggggtccg tgaggaactc gacgccctcg ggcgggacga gatcgccgat gcgcacggaa
     4801 tcgccgatct tcagcttgga gatgtcgaac tcgacccgct gcggcacgtt cgccgggaga
     4861 ctctcgatct ccacctcgcg gaggtgatgc tcgatcaccc caccggcgtg gacgccctcg
     4921 gaatcgccga cctcgtggag ctcgacgctc acggtgatct tctcgtcgcg gttgaccgcc
     4981 atgaaatcga tgtggatgaa ccggttgtgg atcaggtcgc gttggatctc gcgagcgatc
     5041 gtgaggtact cgctcccgtt gggtttcagg tcgacgagga cgctcgcctg acccatcgcg
     5101 tggaagacgt gcaggaggtc gagtgcgctc acgtggatcg gcgtgggatc gacgccggcg
     5161 ccgtacagga ccgcggggac gcggccgctg gcgcggagac gatgcgccgg gcccttcccg
     5221 gtctcggagc ggggttcggc ctgaagtatg gcttccatgg caggtcagag gtcctttttc
     5281 gaaggggtcg atccggttcg ccatcgtacc agcggctagc atctcgcctc gatgccacct
     5341 gcccgcgccg tgggcgcgct gctcgtcctg gccgtgctgg cggcgacctg ccaggaccct
     5401 ccccgtgcga gggacacgcc ggacctcctc ccgcccggga ggccggagcg caccgacgcc
     5461 tccgctttcg acacgcggtg gccgatcaag cacgtggtgt tcgtgatcaa ggagaaccgg
     5521 acgacggatc atctgttcgg ccggttcccc ggagtgaacg gtgtcaccga agggtacgac
     5581 ggcgatgact tgcgaccgct cacgcctccg cccgatcgtc tgcccgacga catccctcac
     5641 tgctacgagt gcggactcga gtcctggaac gaggggcgga tgaacaggtt cgcgcggaac
     5701 gacatcagcg atcgcttcgc gtacacgcag atgcaggggc ccgagcacct ccccaactac
     5761 tggagatggg ccgaggagtt cgcgctgtcg gacaacttct tctcatccgc ccagggcccg
     5821 tcgttcccga atcacctcta cacgatcgcc gcgcagtcgg gcggctcgca ccacaacccc
     5881 ttccaggacc aggcgcgagc gacgctcttc acgcaggaga ccggcctcgc gaagtcgtgg
     5941 gggtgcgaca ccctcgagga ggtctacgtc gaggtggtcg acagcgaggg gcaggtggag
     6001 cgggtgccgc cgtgcttcga cttcccgacc gaagccgacc tccttgacgg ggcgggcatc
     6061 ccgtggggct actacgccgc gtggaacgat cagcgtggct acgtctggtc ggcgttctcc
     6121 gcgatcgagc gcgtgcggat gaacccggcg atctggcagc gccacatctt cccggtggac
     6181 aacttcgttg ccgacgtcga cgacgggcgt ctcgctccgg tcacatgggt cacaccgcgc
     6241 tacgagctga gcgagcaccc cgagtggagc ttctgtcacg gagagaactg gaccaccgag
     6301 atcgtgaacg ccctgatgga gagcgagttc tggcgggacg cggcgatctt catcacgtgg
     6361 gacgactacg gcggct
//
LOCUS       DASUVE010000005         3815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807 k141_1350,
            whole genome shotgun sequence.
ACCESSION   DASUVE010000005 DASUVE010000000
VERSION     DASUVE010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VF036_00100"
     CDS             <1..376
                     /locus_tag="VF036_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263660.1"
                     /translation="RQEPPKRGPAKTEPTTTSDPVVVPEPTPVSERRVAAMAATQASP
                     PPASTAADPESADAEAHEAALEMFIRLTESDRQAHGTTEVDQGAVRAALARTAARKKP
                     GGERLQPHEGPPGESSGGPPGS"
     gene            complement(380..868)
                     /locus_tag="VF036_00105"
     CDS             complement(380..868)
                     /locus_tag="VF036_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263661.1"
                     /translation="MTQYTPRDPLHEPGGFVGVPTHTPRTSRWRSGPTSFGSVGRILC
                     TLALAALFPWWGIGGFNPFFLWALMGWVLAAMFVLRNTWKPERILDPTPTRIERFRVR
                     NPVLGGELRLSGGAGLVVLIFSVGVAASAWLSLDDVSRYAWAVVAIVAGVGFFLASWN
                     DL"
     gene            1048..2364
                     /locus_tag="VF036_00110"
     CDS             1048..2364
                     /locus_tag="VF036_00110"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX6263662.1"
                     /translation="MADLMEAVTALAKRRGIAFQSSEIYGGLRSSWDYGPLGTEIKRN
                     VREQWWRSTVQLRDDVVGLDASVIMSPKVWEASGHLEVFTDPLVECLNCHQRFRADEL
                     PEPTACPNCGQTAFTQPRNFHLMFKTNMGPIEDADNTVYLRPETAQGMFVDFATIQQV
                     SRKKVPFGIAQIGRSFRNEITPGNFIFRQREFEQMEMEFFVEPGTDEDWHEFWIGERM
                     RWWTDLGLREDHVRIREHDADELSHYSKRTVDIEYRFPFTDWGELEGIANRTDFDLRA
                     HQAASGEDLTYFDQEKNERYHPYVIEPAAGVDRAVLAFLLDAYREEEAPTGKGGTEKR
                     TYLALDRRLAPTKVAVLPLSRNEKLAPEARRVFDMVKGEWMCDFDDAGSIGRRYRRQD
                     EVGTPFCVTVDFASLDDAQVTVRERDSMGQDRLPIDGLVDYLTARM"
     gene            2361..2531
                     /locus_tag="VF036_00115"
     CDS             2361..2531
                     /locus_tag="VF036_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263663.1"
                     /translation="MIAYLAAVVLWVAAVVTLVVSTAGFLESTTLLAASAVLSGLAIV
                     AAVSALVVSRRR"
     gene            2528..3811
                     /locus_tag="VF036_00120"
     CDS             2528..3811
                     /locus_tag="VF036_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263664.1"
                     /translation="MRRLAVAIVVLLVACTSEPSPPSPSETPSETPSGDPVEVTTGPT
                     SAVEAMRRLCVAPDVEVDPVTEVPTPPAIAQVADQVQSVRGLEFDRPVNVEPITEVEM
                     DRRLADYLDTYFPKRFYARRSEAWQTIGALPRDVGYLEAIDAYQQGQVLGFYNSQNEE
                     LVYTGDAELNRIEQFVLAHELTHAIDDQHFDLDRLDGLVARCQEERFQAALGIVEGSA
                     NHFATQVLINFPIQEVGSIPEDGTQEVPPFIVDLQAYPYTTGQRFADTLSDANGPSAI
                     DDALETFPTTTEQVMHPTKFPHEVGESLDVPDFAPTFAESWRDLDVMVVGELWLKILL
                     NLRLDDVVAASAAAGWNGGIYRAWTDGDDVAVIMSTVWDTPKDAQEFSDALDRWVSRG
                     TLPGLVMDADGTTVHAGFGSREPLMGAVLSILRSL"
BASE COUNT          657 a         1281 c         1317 g          560 t
ORIGIN      
        1 gaggcaggag ccgccgaagc gggggccggc gaagacggaa ccgaccacca cctcagaccc
       61 ggtcgtggtg ccggagccca cccccgtgtc cgagcgtcgg gtggccgcga tggcggccac
      121 ccaggcgagc ccgcctcccg cgtcgaccgc ggccgatccc gagtccgcgg acgccgaggc
      181 gcacgaggcc gccctcgaga tgttcatccg cctcaccgag agcgaccggc aggcccacgg
      241 cactaccgag gtggaccagg gcgcggttcg cgcggccctc gcccggacgg cggcccgcaa
      301 gaagccgggc ggcgagcggc tgcagccgca tgagggtccg cccggggaat cgtccggcgg
      361 cccgcccggg tcataggtcc tacaggtcgt tccagctcgc gaggaagaag ccgacgcccg
      421 cgacgatcgc cacgacggcc caggcgtagc gggacacatc gtccaaagag agccaggccg
      481 aagctgccac acccacggag aagatcagca cgacaaggcc ggcccctccg ctgaggcgta
      541 gctcccctcc cagcacggga ttgcggacac ggaaccgctc gatcctggtc ggtgtcggat
      601 cgaggatccg ctccggcttc caggtgttcc ggagcacgaa catcgctgcc agcacccatc
      661 ccatcaacgc ccagaggaag aacgggttga atccgccgat accccaccag gggaagagcg
      721 ccgcgagtgc cagggtgcac aagatccggc cgaccgagcc gaacgacgtc ggtcccgacc
      781 gccatcggga ggtcctcggc gtgtgcgtcg gtacgccgac gaagccacct ggctcgtgca
      841 gcggatcgcg gggcgtgtac tgcgtcaccg gcgtgtagca ggtcgtgcac caccggatgt
      901 ccggcgggag gggggcgcca cacgacctgc acagtggtcc cacaggcatg caagacgtat
      961 cgggacggcg gcaccttcga ttgaccccgg tagcatgagc ggcacgccgg cggtcgcagc
     1021 cagcgaccgg gaaggagtcg aagaccaatg gccgacttga tggaggccgt caccgctctc
     1081 gccaaacggc gggggatcgc gttccagtcc tcggagatct acggcgggct ccgctcttct
     1141 tgggactacg ggccgctcgg caccgagatc aagcgcaacg tccgcgagca gtggtggcgt
     1201 tcgaccgtgc agctccgcga cgatgtggtc gggctcgacg cctccgtgat catgtcgccc
     1261 aaggtctggg aggcctccgg ccacctcgag gtcttcacgg accctttggt cgagtgcctg
     1321 aactgccacc aacggttccg cgcggatgag ctccccgagc cgaccgcgtg cccgaattgc
     1381 ggccagacgg ccttcacgca accgcgcaac ttccatctga tgttcaagac gaacatgggc
     1441 ccgatcgagg acgccgacaa cacggtgtac ctgcgacccg agacggcgca ggggatgttc
     1501 gtcgatttcg ccacgatcca acaggtgagt cgcaagaagg tccccttcgg gatcgcccag
     1561 atcggcagat cgttccgcaa cgagatcacc ccggggaact tcatcttccg ccagcgggag
     1621 ttcgagcaga tggagatgga gttcttcgtc gagcccggca ccgacgagga ctggcacgag
     1681 ttctggatcg gcgagcggat gcgctggtgg accgacctcg ggctccgcga ggatcacgtt
     1741 cggatccgcg aacacgacgc cgacgagctc tcgcactact ccaagcgaac cgtcgacatc
     1801 gagtaccgct tcccgttcac cgactggggg gagctcgaag ggatcgcgaa ccgtaccgac
     1861 ttcgacctca gggcgcacca ggcggcctcc ggagaggacc tcacctactt cgaccaggag
     1921 aagaacgagc gataccaccc gtacgtgatc gagcccgcgg cgggcgtcga ccgcgccgtc
     1981 ctcgcgttcc tcctggacgc ctaccgcgag gaggaggcgc cgaccgggaa gggcgggacc
     2041 gagaagcgca cctacctcgc cctcgatcgg aggctggcac cgaccaaggt tgcggtactc
     2101 ccgctgtcgc gcaacgagaa gctcgccccc gaagctcggc gcgtcttcga catggtgaag
     2161 ggcgagtgga tgtgcgactt cgacgacgcg gggtcgatcg ggcgtcgcta tcggcgccag
     2221 gacgaggtcg gcacgccgtt ctgcgtcacg gtcgacttcg cctccctgga cgacgcgcag
     2281 gtcacggtcc gggaacggga ttcgatgggg caggaccgcc tgccgatcga cggcctcgtc
     2341 gactacctga cggcgcgcat gtgatcgcct acctggcggc ggtcgtcctc tgggtggcgg
     2401 cggtcgtgac gctggtcgtc tccaccgcgg ggttcctcga gtccacgacg ctcctcgcgg
     2461 cgtcggcggt gctctcggga ctcgcgatcg tggccgcggt aagcgcgttg gtcgtctcga
     2521 ggcgacggtg aggcggctcg ccgtcgcgat cgtggtgctc ctcgtggctt gcacgtcgga
     2581 gccgtcgcca ccgtcaccgt cggaaacgcc gtcggagacg ccgtcaggcg atccggtcga
     2641 ggtcacgacg gggccgacct cggcggtcga ggccatgcgt cggctctgcg tcgcccccga
     2701 cgtcgaggtg gatcccgtga cggaggtgcc cacgccgccg gcgatcgcgc aggttgccga
     2761 tcaggtgcag tcggtgcgcg gactggagtt cgaccggccg gtgaacgtcg agccgatcac
     2821 ggaagtcgag atggaccggc gattggccga ctacctcgac acgtacttcc cgaaacggtt
     2881 ctacgctcgc aggagcgagg catggcagac gatcggcgcg ttgccgcgcg acgtcggata
     2941 cctggaggcg atcgacgcgt accagcaggg tcaggtcctc ggcttctaca actcgcagaa
     3001 cgaagagctc gtctacaccg gcgatgccga gctgaaccgg atcgagcagt tcgtgctcgc
     3061 ccatgagctc acgcacgcga tcgacgacca gcatttcgac ctcgaccgcc tcgacggtct
     3121 cgtggcgcga tgccaagagg agcgtttcca agccgctctc ggtatcgtgg aggggagcgc
     3181 gaaccacttc gccacgcaag tgctgatcaa cttcccgatc caggaggtcg ggtcgatccc
     3241 ggaggacggg acccaggagg tcccgccgtt catcgtcgac ttgcaggcct acccgtacac
     3301 cacgggtcag cggttcgcgg acacgctctc cgacgcgaac ggaccctcgg cgatcgacga
     3361 cgcgttggag acgttcccga ccacgacgga gcaggtgatg catcccacga agttcccgca
     3421 cgaggtgggg gagtccctcg acgtgccgga cttcgcgccc acgttcgcag agagctggcg
     3481 cgacctggac gtgatggtgg tgggcgagct gtggctgaag atcttgctga atctccgcct
     3541 cgatgacgtc gtggcggctt cggcggcggc tggatggaac ggcgggatct accgcgcctg
     3601 gaccgacggc gacgacgtgg cggtcatcat gagcaccgtc tgggacacgc cgaaggacgc
     3661 gcaggagttc agcgacgcgc tcgatcgctg ggtgtcacga ggcacgctcc ccggcctggt
     3721 gatggatgcc gacggcacga cggtgcacgc gggcttcggc tcccgggagc ccctgatggg
     3781 cgcggtcctg tcgatcctgc ggtcgttgta gatgc
//
LOCUS       DASUVE010000006         5555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807 k141_1364,
            whole genome shotgun sequence.
ACCESSION   DASUVE010000006 DASUVE010000000
VERSION     DASUVE010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..2695)
                     /locus_tag="VF036_00125"
     CDS             complement(125..2695)
                     /locus_tag="VF036_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX6263665.1"
                     /translation="MTGPDPVAHGIDRAGRDRARDGLTADEAEARLDRIGPNEIPTGP
                     RASWRRRFARQLLEPMALLLMVAAAVEGFGLGERLEAIAILAIVFLNAVIGTVQEGRA
                     ERALDALRSMEPRYATVIRDARRQRIDLARVVPGDIAVLGAGDRVPADLELLVVNGLQ
                     VDESVLTGESLPVDKLVPTTVEDDDNGRAFAGTVVTNGEGLGVVVATGASSSLGRIAA
                     ELGEARAPTPLQRRLRRLTKTLGLAAVAIAAGVFVLTLVRLGTGEDQVRQAFLSAVAL
                     AVAAVPEGLPAVVTVVLALGVRRMAERGAIVRRLPAVETLGSSTVILTDKTGTLTENR
                     MQVDRIVVAGEAPTRAIVGSVPERLATAIALGSDATLEPPTGDPLEVALLESVGEAEV
                     RRIRDRQRRLGSLPFDASRRRTVTVNEGTGTAVAVLKGAPEVVLGASVDAVDRDGGRG
                     PLAQDAILRSAEDLATSGSRVIAFAVREGSDVDPDRPPEEGFTFLGLVGLRDPVRVEA
                     APAIADATSAGIRIVMVTGDHAGTAASVSREVGLLAPDGHVLGGDQLGQADPGEVSVF
                     ARVKPDDKLAIVRALQGRGDVVAVTGDGVNDAPALRQADIGVAMGRTGSDVAREAADM
                     VITDDNLGTIVHAVEEGRQLVRNIRNVIDYLVAGNLSEVAVVVTVLLLYPDAGATLTP
                     LQILWINLLTDGLPAVALGVESGSGVRTDRVDTSSLLSWRRARMLAVRGMAIAFTSVG
                     TFVVVRFGFDQSFESARTAMFTVLVVSHLLYAFAVYLDRPGRAPVPLRALLAARGLLI
                     AVGSGIILQLAVVAVSLLHDVFGTTSMTPSEWLVCLSVGLVAPIGIWLASRVGWRSTA
                     "
     gene            complement(2692..5292)
                     /locus_tag="VF036_00130"
     CDS             complement(2692..5292)
                     /locus_tag="VF036_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX6263666.1"
                     /translation="MQQLERREPEGLSEAAAAARLLRDGPNVLPIRRPRPLWLEAVGQ
                     LTHFFALMLWAAGLLAFVAGLPQLGVAIFVVVLANGAFAFVQEHRAQRAAERLRDLLP
                     RRATVVRGGRHLEIDAAELVVGDLVLLEAGDRISADLRIERAAAAMMGTAALTGESVP
                     TAVDVGDEALAGTFLVEGEVIGEVTATGTATRLAGIAALAAASHRPRSPLSLELRRVV
                     RAISTIALLVGASFFGVALLVGIAPSEGFLFAIGVVVALVPEGLLPTVTLSLAIGAQR
                     MARHQALVRRLDAVETLGSATFICTDKTGTLTWNEMSVVEVWTPGGTARIHGQGYEPT
                     ATVETDPEMSEDALPRIALVAARCSTGHADLRDGVWTAVGDPMDAAIDALTRRVGVDV
                     SADRAAAPEVARFPFDPRRRRVSSAGARHVYVKGAADSVLPLCHHEPGADVAMEALAA
                     RGLRVIAVATRPASTRPRDAGDAERDLELLGFIGLLDPPRDDVRESIASCRRAGVRIA
                     MVTGDHPATAAAVARAVGLASEDDPVLVGDDLPEDEQILAAMIDRDGIVLARVSPEQK
                     VRIARALRSRGHVVAMTGDGVNDGPALQAADIGVAMGRSGTDVAREASDLVLLDDHFD
                     TIVTAIEHGRVTFNNVQKFLTYHLTDNVAELTPFAVWALSGGRIPLALSVMQILVLDL
                     ATDTFSAVALGAEPPVAGALTSTTPVRGRLLDRVVAIRAFAIAGPTEAVFEMGAFFAS
                     LFAFGWRPGAPFPSGMALAAASGAAFVVVVLAQTANAFACRSSTLPPWRIGWLTNRVL
                     LLGGSIELVLAAAFLLWPALGEPLDHRPPPVIGWVIAVASMPALLLIDASFKRRFRRR
                     LASAEAAVSA"
BASE COUNT          922 a         2038 c         1842 g          753 t
ORIGIN      
        1 cggcttctct cgttgtcccc ctattggatc acggtggtga gcggcacgat cgtggcgatc
       61 gaggagcagt tcatccccta gcctctccgg gaacggcact gaggatcggg atgcatctcc
      121 agcttcacgc cgtactccgc cacccgaccc ggctggccag ccagatcccg atgggcgcga
      181 cgagccctac tgacaggcac accagccatt cggacggtgt catggatgtg gtgccgaaga
      241 catcgtggag gagcgagacc gccaccacgg cgagctgcag gatgatgcca ctgcccacgg
      301 cgatcaacag ccctcgtgca gccagcagcg cacgaagcgg caccggcgct cggcccggac
      361 gatcgaggta gacggcgaat gcgtagagca ggtgagacac cacgaggacg gtgaacatcg
      421 ccgtccgtgc ggactcgaac gactggtcga agccgaaccg gacgaccacg aaggtgccca
      481 cggacgtgaa cgcgatcgcc atcccgcgta ccgcgagcat ccgcgctctc cgccacgaca
      541 gcaagctcga ggtgtccacc cggtccgtcc gtaccccgga ccccgactcg accccgagag
      601 ccaccgccgg aagaccatcg gtgaggaggt tgatccagag gatctgcagg ggcgtgagcg
      661 tggcaccggc gtcgggatac aggagcagca ccgtcacgac gacggcgacc tcggacaggt
      721 tcccggcgac gagatagtcg atcacgttgc ggatgttcct gacgagctgg cgtccctcct
      781 cgacggcatg gacgatcgtg ccgaggttgt cgtcggtgat caccatgtcg gcggcttccc
      841 gtgcgacgtc gctgcccgtc ctgcccatcg cgacaccgat atctgcctgc cgcagtgcgg
      901 gagcgtcgtt cacaccgtca ccggtcaccg cgacgacatc tccccgcccc tggagcgcgc
      961 ggacgatcgc gagcttgtca tccggcttca cgcgcgcgaa cacgctgacc tctcccggat
     1021 ccgcctggcc gagctgatcg cccccgagga catgaccgtc gggcgcgagg aggccaacct
     1081 cgcgcgagac cgacgccgcc gtgcccgcgt ggtctccggt gaccatcacg atccggatgc
     1141 cggcggaggt cgcgtcggcg atcgccggtg ctgcctccac ccggacggga tcgcggagac
     1201 ccacgaggcc gaggaacgtg aacccctcct cgggcggacg atccgggtcg acatccgatc
     1261 cctcccggac ggcgaaggcg atcacacggc tccccgaggt ggcgaggtcc tctgcagacc
     1321 gaaggatcgc gtcttgcgcc agtggacctc ggccgccgtc gcggtcgacc gcgtcgaccg
     1381 atgcgcccag gacgacctcg ggagcgccct tgagaacggc gaccgccgtg cccgtccctt
     1441 cgttcaccgt gaccgttcgc cgccgactgg catcgaacgg gagggagccg agccggcgct
     1501 gccgatcacg gatgcggcga acctccgcct cccccaccga ttccaggagc gcgacctcga
     1561 gcgggtcgcc ggtcggaggc tccagcgtcg cgtccgatcc gagggcgatc gcggtcgcca
     1621 agcgttccgg cacggacccc acgatcgccc gcgttggggc ctcacccgcc acgacgatgc
     1681 gatccacctg catccggttc tctgtgagcg tcccggtctt gtccgtcagg attaccgtcg
     1741 acgagcccag ggtctcgacc gcgggcagac gccggacgat cgcgccccgc tcggccatgc
     1801 gtcggacgcc gagagcgagt acaacggtca ccaccgccgg cagcccttcc gggacggctg
     1861 cgacagctaa cgcgaccgcg gacaggaagg cctgcctgac ctggtcctct ccggtcccaa
     1921 gccggacgag ggtgagcacg aagacgccgg cggcgatcgc caccgccgcc aggccgaggg
     1981 tcttcgtcag tcggcggagt cggcgttgca agggtgtggg ggcacgcgct tcgccgagct
     2041 cggccgcgat ccggcccaac gagctggagg cgcccgtcgc cacgacgact cccagtccct
     2101 ccccgttcgt cacgacggtt cccgcgaagg cgcgcccgtt gtcgtcatcc tcgaccgtgg
     2161 tgggcacgag cttgtcgacg ggcagcgatt cgcccgtcag gactgactcg tccacctgga
     2221 ggccgttcac gaccagcagc tcgaggtcag cggggacgcg gtcgccggcg cccaacacgg
     2281 cgatgtcgcc cggaaccact cgcgcgaggt cgatccgttg ccggcgcgcg tctcgaatca
     2341 ccgtggcgta gcgaggctcc atcgagcgca acgcgtcgag agctcgttcc gcgcgtccct
     2401 cctggacagt cccgatcacg gcgttcagga agacgatggc caggatcgcg atcgcttcga
     2461 ggcgctcccc gagcccgaac ccctccaccg ccgcggccac catgagcaag agcgccatcg
     2521 gctccaggag ctggcgggcg aagcgccgcc gccacgacgc acgagggccc gtcgggatct
     2581 cgttcggccc gatacggtcg agtcgggctt ccgcctcgtc ggccgtcaga ccgtctcgag
     2641 cgcggtcgcg accggcgcga tcgatcccgt gtgcgacggg gtccggaccg gtcacgcgga
     2701 cactgccgct tccgcggaag cgagacgacg acggaagcga cgcttgaagg acgcgtcgat
     2761 caacaacagg gccggcatcg acgccaccgc gatgacccac ccgatgacgg gagggggacg
     2821 atggtcgagg ggttctccga gggcgggcca gagcaggaac gccgccgcga gcaccagctc
     2881 gatcgacccc ccgaggagga gcacccggtt cgtcagccat ccgatccgcc acgggggcag
     2941 ggtggaactc ctgcacgcga acgcgttcgc cgtctgtgcg aggaccacta ccacgaacgc
     3001 cgctcccgag gcggccgcca gggccatccc ggaggggaac ggcgccccag ggcgccaccc
     3061 gaacgcgaac aacgaagcga agaacgctcc catctcgaag accgcttcgg tgggacccgc
     3121 tatcgcgaac gctcggatcg cgaccaccct gtcgagcaac cgaccccgaa ccggggtggt
     3181 cgacgtcagc gcgcccgcca ccggcggctc ggcgccgagc gcgaccgccg agaaggtgtc
     3241 cgtcgcgaga tcgagcacca ggatctgcat cacggacagc gcgagcggga tccttccgcc
     3301 cgagagggcc cacacggcga acggcgtgag ctcggccacg ttgtcggtga ggtgatacgt
     3361 gaggaacttc tgcacgttgt tgaacgtcac ccgaccgtgc tcgatcgccg tgacgatcgt
     3421 gtcgaaatga tcgtcgagca ggacgagatc ggaagcctca cgggccacgt ctgtgcccga
     3481 tcgccccatc gcgacgccga tgtctgctgc ttggagagcg ggcccgtcgt tcacaccgtc
     3541 gccggtcatc gccacgacgt gcccgcgtga tcgcagcgct ctggcgatgc ggaccttctg
     3601 ctccggcgac acccgagcga gcacgatccc atcgcggtcg atcatcgcgg cgaggatctg
     3661 ctcgtcttcg ggcaggtcgt caccgaccag gacgggatcg tcctccgacg cgagcccgac
     3721 ggctcgagcg accgccgccg ccgtcgcggg atgatctccg gtcaccatcg cgatccgcac
     3781 ccccgcccgc cggcacgatg cgatggactc ccggacgtca tctcggggcg ggtcgaggag
     3841 tccgatgaat ccgaggagct ccagatctcg ctcggcgtcg cccgcatccc gcggacgggt
     3901 cgaggccggt cgtgtcgcga cggcgatcac ccggagccca cgagcggcca acgcctccat
     3961 cgcgacgtcc gcgccgggtt cgtgatggca caggggcagc acgctgtcgg ccgcaccctt
     4021 gacgtacaca tggcgagcac cagcgctcga cacccggcgc cgcctcggat cgaacgggaa
     4081 tcgtgcaact tccggagctg ccgccctgtc ggccgaaaca tccaccccga cccgccgtgt
     4141 gagcgcatcg atcgcggcat ccatcgggtc cccgacggcg gtccacacgc cgtctcggag
     4201 gtccgcgtga cccgtcgagc atcgagcggc caccagagcg atccggggga gggcgtcctc
     4261 gctcatctcc ggatccgtct ccaccgtggc cgtgggctcg tagccctgcc catggatccg
     4321 cgcggttccg cccggcgtcc acacctcgac caccgacatc tcgttccagg tgagcgtgcc
     4381 cgtcttgtcg gtgcagatga acgtggcgga gccgagcgtc tcgaccgcat cgagccggcg
     4441 gacgagcgct tggtgtcgcg ccatgcgttg cgcgccgatc gcgagggaca gggtgacggt
     4501 cggcaggagg ccctccggga cgagcgcaac cacgacgccg atcgcgaaca ggaacccctc
     4561 gctcggcgcg atcccgacca acaacgccac gccgaagaac gaggcaccca ccaggagggc
     4621 gatcgtggag atcgctcgga ccactcggcg cagctcgagg ctgagcggcg accgcggccg
     4681 atggctcgcg gccgcgagcg ccgcgatccc ggcaagtctg gtagccgtcc ccgtcgccgt
     4741 gacttcgccg atgacttcac cttcgacgag gaacgtcccc gcgagcgcct catcgccgac
     4801 gtcgactgcc gtggggacgc tctcccccgt cagggccgcc gtccccatca tcgccgccgc
     4861 cgcacgctcg atccgcagat cggcggagat gcggtcaccc gcctccagga ggacgagatc
     4921 gcccaccacc agctcggcag catcgatctc gaggtgtcgc ccgccgcgga cgacggtcgc
     4981 tcgcctcggg agcagatcgc ggagtcgctc cgccgcgcgc tgcgcacgat gctcctgcac
     5041 gaacgcgaac gcgccgttcg cgagcacgac gacgaagatc gcgacgccta gctgcggcaa
     5101 ccccgcaacg aacgcgagga gcccggcggc ccagagcatc agcgcgaaga agtgcgtgag
     5161 ctgtccgaca gcctcgagcc acaacggacg agggcgacgg atcggaagga cgttgggacc
     5221 gtcccgaagc aggcgggccg ccgccgcggc ctcgctcaac ccttcgggct cacgacgttc
     5281 gagctgctgc acggccgccg ctaccctact ggggagacgg ccgaggttcc tccggagcct
     5341 cggccgccgg atccccctgg cggttcgcct ggatgcgtct caggcggcct tggcaggctt
     5401 cttccccgac aacaccggga tcttacgagc ctcgacagcc ttctccgcct ccttcaccgc
     5461 gcccttcgga acgaccacct cgagcacgcc gtcggcgtac gtcgcggaga tgcggctctc
     5521 gtcgaccccc tcggggatcg cgaactgccg ctcga
//
LOCUS       DASUVE010000007         3669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1508742, whole genome shotgun sequence.
ACCESSION   DASUVE010000007 DASUVE010000000
VERSION     DASUVE010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508742"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..2219
                     /locus_tag="VF036_00135"
     CDS             39..2219
                     /locus_tag="VF036_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6263667.1"
                     /translation="MPGHPHPRASERLDPFASGSRVRELVEQLPVVVYVDTDHQRPST
                     TYISPNVEQLLGFPPQRFLEDPGLWIRCMHPDDRERIQTLRDEVWSSGARYRSEYRMF
                     RRDGEEVWLRDSSVLVVSQEGERLAWQGVIEDITTERRAEQEIRASEARYRALVERVP
                     VVVYEMGPDDERQTLFVSPHVEQVLGYSREEWLDQPDIWIELLHVDDREVVLAKHDRH
                     NETGEPWDLEYRLIASDGRVVWVHDRASLIRGLERGGAAWHGVMIDITAEHEATEMLL
                     LHKEDLERRVAERTSELQEANELMALEIGERRRMEGELRDIEERYRRLVEHMPGIAYV
                     WDVRPGAEDRTFGYVSPRIHDILGFSPEEWHASARVHPHDQARVSEAVARSVRTGEPF
                     AMEYRYLAKDGSVVWVLDHASLITRSDAGDPASFQGMMLDVTSRMDAETKAAAAEDRY
                     RTLTERGPVVAYSYELTYADEAGPSVHVSYLSPQAAELVHQPIEHWLHEPEVWLGMIH
                     PDDRDRIAQTTAHTWLTGEPWMVRYRMIRSDGTVIWLLDAGRLLDRDAQGRPWTFQGI
                     LLDVTTDEEARTQAEVTARAQRDALEGVLAIPWSETIHPETGFERYTYIGSQASDILG
                     YTPEELTDEPKHFPRMVHPDDRPRVQRVVQRSDETGVWDDTYRILRRDGEVRWLHSFG
                     RRTSAPGVIPELWHGVAIDVTGYRAEAGADEGLVAGDAVIDRAP"
     gene            complement(2236..3510)
                     /gene="serS"
                     /locus_tag="VF036_00140"
     CDS             complement(2236..3510)
                     /gene="serS"
                     /locus_tag="VF036_00140"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX6263668.1"
                     /translation="MLDIRRIREDPDPFRAALARRNLGDAVDRLLEADERRRALTSRV
                     EELRAAQNRASKAIGAAQGDEKQRLIDEVAKVSIELKELDPALAAAEEDLRSLLAETP
                     NLPHESAPDGFTDEDAVEIRRNHEEPPVLGFEPRDHAELGAMLGVLDTERGARTSGSR
                     FVYLMGDLVFVQFALMRHAMDVVAERGFSPMIPPVLVREEAMYGTGFLPTDAVNIYVT
                     REDDLYLAGTSEVPLAAFHLGEILDEADLPIRYAGYSSCFRREAGSYGKDLGGMFRVH
                     QFDKVEMFAFCAPETSWDEHERILAIEEEILGNLEIPYRVVNVAAGDLGGAAAKKYDI
                     EGWLPAQQRYRELTSCSNTTDYQARRMQTRLRRAGGDVEVLHTLNGTATAIGRTLIAI
                     MENHQRPDGSVEIPPKLHPYLPERARVLAPRP"
BASE COUNT          599 a         1250 c         1246 g          574 t
ORIGIN      
        1 cctggagtgg gagataccgg tcgaggtacc ctggctcgat gccgggccac ccgcatcccc
       61 gcgcctccga gcgtctcgat ccgttcgcgt ccggctcccg ggtccgagag ctcgtggagc
      121 agctccccgt cgtcgtctac gtggacaccg atcaccagcg cccctcgacg acctacatca
      181 gccccaacgt cgaacaactc ctggggttcc ctccgcagcg gttcctggag gatccgggcc
      241 tctggatccg ctgcatgcac ccggacgacc gggagcggat ccagacgctc cgagacgagg
      301 tctggtcgag cggcgcgcgc taccggtcgg agtaccggat gttccgccgc gacggcgagg
      361 aggtgtggct ccgagactcg agcgtcctcg tcgttagcca ggaaggcgaa cgtctcgcgt
      421 ggcagggcgt gatcgaggac atcacgaccg agcggcgagc cgagcaggag atccgtgcat
      481 ccgaggctcg ctatcgagcg ctcgtcgagc gggtgccggt ggtcgtctac gagatgggcc
      541 ccgacgacga acgccagacg ctgttcgtga gcccgcacgt cgaacaggtc cttgggtact
      601 cgcgcgaaga atggctcgac cagccggata tctggatcga gctcctccac gtggacgatc
      661 gtgaggtcgt cctcgcgaag cacgaccgcc ataacgagac gggtgagccg tgggacctgg
      721 agtaccggtt gatcgcgagc gacgggaggg tcgtctgggt ccacgaccgc gcgagcttga
      781 tccggggtct ggagcgcggc ggggccgcgt ggcacggcgt gatgatcgat atcacggcgg
      841 agcacgaggc tacggagatg ctgctcctcc acaaggaaga cctggagcgg cgcgtcgcgg
      901 agcgaaccag tgagctgcag gaagccaacg agctgatggc gctcgagatc ggagagcgcc
      961 ggcggatgga aggcgagctc cgtgacatcg aggaacgcta ccgccggctc gtcgagcaca
     1021 tgcccggcat cgcgtacgtc tgggatgtcc gtcccggcgc cgaggaccga accttcggat
     1081 acgtgagccc tcggatccac gacatcctcg ggttctcccc cgaggagtgg cacgcatcgg
     1141 cccgagtcca cccacacgac caagcgcgtg tctccgaagc cgtcgcccgg agcgtgcgga
     1201 ccggcgagcc cttcgccatg gagtaccgat acctcgcgaa ggacgggagc gtcgtctggg
     1261 tcctggatca tgcctcgctg atcacgcgat ccgacgccgg agaccccgct tcgttccagg
     1321 gcatgatgct ggatgtcacg agtcgcatgg acgccgaaac caaggcggcg gccgcggagg
     1381 atcgctaccg gacgctcacc gaacgaggcc cggtcgtcgc ctactcctac gagctgacct
     1441 acgccgacga ggccggcccc tccgtccatg tctcgtacct gagcccgcag gccgcggagc
     1501 tcgtgcacca gccgatcgag cactggctcc atgagcctga ggtctggttg gggatgatcc
     1561 atccggacga tcgcgaccgc atcgcccaga cgacggcgca cacctggctg acgggcgaac
     1621 cgtggatggt ccgctatcgc atgatccgct ccgacggaac agtgatctgg ctcctcgacg
     1681 ccggccgcct cctcgatcgg gacgcgcaag ggaggccctg gaccttccag gggatcctcc
     1741 tcgacgtgac cacggacgaa gaagcgagaa cccaggccga ggtgacggcg cgagcgcagc
     1801 gcgatgcgct cgagggggtg ctcgcgatcc cgtggtccga gacgatccac cccgagacgg
     1861 ggttcgagcg ctacacgtac atcgggtctc aggcctccga catcctcgga tacacgcccg
     1921 aggagctcac ggacgagccg aagcacttcc ctcggatggt gcatccggac gatcgacctc
     1981 gggtccaacg ggtggtgcag cgctccgatg agacgggcgt gtgggacgac acctatcgga
     2041 tcctgcgtcg cgacggcgag gtccggtggc tccactcgtt cgggcggcgc acctcggctc
     2101 ccggcgtgat ccccgagctc tggcacggcg tcgcgatcga cgtgaccggg taccgagcgg
     2161 aagccggagc ggacgaaggg ctcgtggcgg gcgacgcggt gatcgaccga gctccgtaga
     2221 actccgtcgc gggggtcagg gccgaggagc cagcacgcgc gcgcgctccg gcagatacgg
     2281 atgcagcttc ggggggatct ccacgcttcc gtcggggcgc tggtggttct ccatgatcgc
     2341 gatcaacgtg cgaccgatcg ccgtggcggt gccgttcaaa gtgtggagga cctccacgtc
     2401 cccccccgct cgacggagcc gcgtctgcat ccggcgagcc tggtagtcgg tcgtgttcga
     2461 gcacgaggtg agctcgcggt agcgctgctg cgccggcagc cagccctcga tgtcgtactt
     2521 cttggccgcg gccccaccga gatcgccggc cgcgacgttc accacccggt aggggatctc
     2581 caggttcccc aggatctcct cctcgatcgc gaggatgcgc tcgtgctcat cccacgacgt
     2641 ctcgggcgcg cagaacgcga acatctccac cttgtcgaac tggtggacgc ggaacatgcc
     2701 ccccaggtcc ttgccgtacg aacccgcctc gcgccggaag cagctcgagt agccagcgta
     2761 gcggatcggg aggtcggcct cgtcgaggat ctcaccgagg tggaacgcgg ccaggggcac
     2821 ctccgaggtg cccgcgagat agaggtcgtc ttcgcgcgtc acgtagatgt tcacggcgtc
     2881 ggtgggcagg aatccggtgc cgtacatcgc ctcctcgcgg acgaggaccg gcgggatcat
     2941 cggcgagaac cccctctcgg cgacgacgtc catcgcgtgg cgcatcagcg cgaactgcac
     3001 gaagacgaga tctcccatca gatagacgaa gcgggagccg gacgtccgcg cgccgcgctc
     3061 ggtgtccagc acaccgagca tcgcgccgag ctccgcgtga tcgcgaggct cgaacccgag
     3121 gaccggcggt tcctcatggt tgcgtctgat ctcgacagcg tcctcgtccg tgaacccgtc
     3181 gggggcgctc tcgtgcggaa ggttgggggt ctccgcgagg agcgaacgga ggtcctcctc
     3241 ggccgcggcc agcgccgggt ccagctcctt gagctcgatg gagaccttcg cgacctcgtc
     3301 gatcagccgc tgcttctcgt caccctgtgc ggcgccgatc gccttcgagg cacggttctg
     3361 cgccgctcgc agctcctcga cgcgggacgt gagcgctcgg cgccgctcgt ccgcctcgag
     3421 cagacggtcc acggcgtcac cgaggttccg cctcgcgagt gcggcccgga acggatcggg
     3481 atcctccctg atccgtctga tgtcgagcaa ctacggaacc ttcacttccg gttcgtctcg
     3541 cttctcctct gcgacctgag gcgccttctc ggtcttcgcc ggtgcctcct gagaacgagc
     3601 gccctcggtg ggagccgagc cgtccatgat gctctgcccg tggaacacgc cgccctcctc
     3661 gaccacgag
//
LOCUS       DASUVE010000008         5094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_755399, whole genome shotgun sequence.
ACCESSION   DASUVE010000008 DASUVE010000000
VERSION     DASUVE010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755399"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VF036_00145"
     CDS             <1..1016
                     /locus_tag="VF036_00145"
                     /inference="COORDINATES: protein motif:HMM:NF040160.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HEX6263669.1"
                     /translation="GAGWVSSTDAGAAAEPCAAEASQRWYLADGSTELGEESYVVVAN
                     PFSGPAVMDVVLFSADQPPVRHSDWTDLVVPAGRSVALHLNQRLKGEPVVATTLEVSV
                     GRVAAASLVVSDRTTVRSTIGWTEPATEAWFPTMKGSGQNELVLVSTASASIRFGATS
                     LSEEEPRPAGGLTEQEHGPTAARAYAVPEEAGPTAVRLFTLEGALVSGALRVLGPGTD
                     VGSTGGSASASEAWVLFPSSARIPAEPGALLVNDGEVPVAASIELLPEEGGTPPSSVT
                     ITVPPHATGAVPPDLWATAPGSALVVRADGPLLALSAATSPGSRDAEAFALSMGVPLP
                     LLP"
     gene            1105..2241
                     /locus_tag="VF036_00150"
     CDS             1105..2241
                     /locus_tag="VF036_00150"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX6263670.1"
                     /translation="MAKGRHEVRAARKRWPLIVGLVAGVLVLALGGVAYATFRYEQAS
                     AGRMLAGTTIGGVNVSGMTRAEAIAVVRAHAREDLNRQIVVVVRGERFTATARQLGQR
                     PWVGQAVDEAMDAAERVGWVERAWRRFRNDPVDLDIELSMAGDRGVAGFVRRTARQVR
                     VDPRNASIGMGEGGGLFFRHAKAGRELEVRRAERLLTQALDDGSATVRLSVRSVAPKF
                     TEDTMLPTVVVHVDTNRLDLYEGFDVTHSWDVATAKPGWTTPVGEWSLYQKRENPTWY
                     NPALDSWGAGMPAVIPGGPGNPMGTRALYITAPGLIRIHGTTSPDSIGRYASHGCIRM
                     HNEQVEELYDLVPVGTKVIIMGSRPSWASEGDFPDTPDGSDTVA"
     gene            2578..2757
                     /locus_tag="VF036_00155"
     CDS             2578..2757
                     /locus_tag="VF036_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263671.1"
                     /translation="MPQANTCPECKQGDVIAISMNVSGRDLAFSTCHACEAKWWHRDG
                     EQIVLSDVIETVVRT"
     gene            2787..4190
                     /gene="manB"
                     /locus_tag="VF036_00160"
     CDS             2787..4190
                     /gene="manB"
                     /locus_tag="VF036_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960675.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX6263672.1"
                     /translation="MAPVDLSGIFKAYDVRGTVPDQLNADVVRRIGAAFADWSGANEI
                     LVGRDCRLSSPELAATLIEGATSRGVDVVDLGLASTDLLYFASGSLDRPGVMITASHN
                     PKDYNGLKFCLARAKPVGEESGLGEIRARTEKGDDILGAGNGSVRQRDVLGAYVDHVL
                     SFTDVDRMRPLTVAVDTANGMGGLVVPAVMERLPVTLHHLYAELDGTFPNHPADPIDP
                     ANQRDLKRAVLEHGADVGMAFDGDADRVFLVDERADDVSGSILTALVAAAMLDREPGA
                     QVVHNLICSWTVPEVIKEHGGVPVRTRVGHSFIKQVMAETGAVFGGEHSGHYYFRDNY
                     GADSGLIASVIALGELSDASVPLSGLLGPYRRYASSGEINSRVDDPLTRIEDIARALR
                     DGRQDRLDGLTVEFDDWWCNVRPSNTEPLLRLNVEARTDEILAASAAELLALIRGTGD
                     AGGNAPGTSGEGGKDEP"
     gene            4187..4381
                     /locus_tag="VF036_00165"
     CDS             4187..4381
                     /locus_tag="VF036_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX6263673.1"
                     /translation="MTLDAELLEILVCPNDRGDIDYHEDEQVIVCRTCGYRYPVRDGI
                     PVMLIDEAEKPGGGPGIKNG"
     gene            4378..>5094
                     /locus_tag="VF036_00170"
     CDS             4378..>5094
                     /locus_tag="VF036_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX6263674.1"
                     /translation="MTIDLDDPEAVRAHDPGGMLAAAAQLASHCREGYANGTGAGALP
                     DLVDVRNVVYCGMGGSAVAGDVLRSVFRDRLGVPVEVNRSSELPEHAGPHSLVVACSY
                     SGNTSETLAAFREAMKRGCRSVVITSGGAIADEAGEAGVPVVRVPGGMQPRAALGWLG
                     CTALGALETAGLLPPLAEDIAEVADEVQARAATCDAGRPVANNPAKLLAEEIGERIPV
                     IWGAEGIGAVAAARWKTQMNE"
BASE COUNT          758 a         1704 c         1854 g          778 t
ORIGIN      
        1 tcggcgcggg atgggtgtcg tccacggacg cgggggccgc cgcggagccg tgcgctgccg
       61 aggcgtcgca gcggtggtac ctcgcggacg ggtccaccga gctaggcgag gaatcgtacg
      121 tggtcgtcgc caacccgttc tccgggcccg cggtgatgga cgtggtcctc ttttcggcgg
      181 accagccgcc cgtccgtcac tccgactgga ccgacctcgt cgtgccggcc gggcggagcg
      241 tcgccctgca tctcaaccaa cggttgaaag gagagccggt ggtcgcgacg acgctcgagg
      301 tctcggtcgg gcgggtcgcg gccgcatccc tcgtcgtgag cgaccgcacg acggtgcgca
      361 gcacgatcgg atggaccgag cccgccaccg aagcgtggtt cccgacgatg aaggggtccg
      421 gtcagaacga gctggtcctc gtgtccacgg cgtcggcgtc gatccgattc ggcgccacct
      481 cactcagcga ggaggagccg cggccggcgg gcgggctcac cgaacaggag cacggtccga
      541 ccgcggcccg cgcctacgcc gtccccgagg aggccgggcc caccgcggtc cggctcttca
      601 cgctcgaggg cgctctcgtc tcgggagcgc tgcgcgtgtt gggaccgggg accgacgtcg
      661 gctccacggg aggctcggcc tcggcgtctg aggcctgggt gctctttcct tcttcggcac
      721 gcatcccggc cgagccgggg gcgttgctcg tgaatgacgg tgaggttccc gtggcggcct
      781 ccatcgagct cctcccggag gaggggggca cgccaccctc ctccgtcacg atcaccgtcc
      841 ccccgcacgc gacgggcgcc gtgccgccgg acttgtgggc gacggccccc ggctccgccc
      901 tcgtggtccg ggcggacgga cccctcctgg cgctctcggc cgcgacctcc ccggggtctc
      961 gcgacgccga ggcgttcgcc ctcagcatgg gggtgccgct gccgcttctg ccgtagccct
     1021 ggcgcaccag gggggagggg ttgtctcctg gcgtatcatc ttcgttccgg gtcccgatcc
     1081 cggaacgaag aacacatcct ccccatggcc aaaggacgtc acgaagtgcg tgcggccagg
     1141 aagcgatggc cgctgatcgt cggtctcgtc gccggcgtgc tcgtgctcgc tctgggtggg
     1201 gtggcctacg cgacgttccg ctacgagcag gcgagcgccg ggcgcatgct cgccggcacc
     1261 accatcggcg gcgtgaacgt ctccgggatg acccgggcgg aggcgatcgc ggtcgtccgc
     1321 gcgcacgccc gagaagacct caaccgacag atcgtggtcg tcgtgcgggg cgaacgcttc
     1381 accgcgacgg ctcggcagtt gggccagcgc ccgtgggtcg gccaggcggt cgacgaggcg
     1441 atggatgccg ccgagcgggt cggctgggtc gaacgagcct ggcgacgctt ccggaacgac
     1501 cccgtcgacc tcgacatcga gctcagcatg gccggggacc gcggcgtggc gggattcgtc
     1561 cgtcgcacgg cgcggcaggt gcgcgtcgat cctcggaacg cgtcgatcgg catgggagag
     1621 ggcggcggct tgttcttccg acacgcgaag gcgggcaggg agctggaggt ccgtcgcgcg
     1681 gagcggctcc tcacccaagc gctcgacgac ggatcggcga cggtccggct gtcggtgcga
     1741 tcggtggcac cgaagttcac cgaggacacg atgctgccga cggtcgtcgt gcacgtcgac
     1801 acgaaccgac tcgacctcta cgaggggttc gacgtgacgc acagctggga cgtcgccacc
     1861 gcgaagccgg gatggaccac gccggtcggc gagtggtccc tgtatcagaa gcgcgagaac
     1921 ccgacgtggt acaacccggc gctcgacagc tggggcgccg gtatgcccgc cgtgatcccc
     1981 ggcggacccg gcaacccgat gggcacgcgg gccctctaca tcaccgcccc cggtctcatc
     2041 cggatccacg ggaccacctc gcccgattcg atcggtcggt atgcgtccca tggctgcatc
     2101 cggatgcaca acgagcaggt ggaagagctc tacgacctcg tgcccgtggg gacgaaggtc
     2161 atcatcatgg gctcgcggcc ttcgtgggcg tccgaagggg acttccccga cacccccgac
     2221 ggctccgaca ccgtcgcctg atcgcgcggt cctcgcatat ggttcagggc ggtcgcctgt
     2281 cgcctcatgc cccggcacgt gtctccatga gtccgcgagc cggccggctg gtggctgaca
     2341 ggcggttcgt cgctgctgag tcgaccgggc tgactagctc tttctggcta gacggatcgg
     2401 cgttcggtat cccgcacgag cgcgcgtcgg gggacccgag aatgactccc aggtgtctgc
     2461 agttcgtggg ggtgcccctg ataccgtccg tcgatcagct ggatcggttt cccccaacag
     2521 atcccgaacg ggcctcgcag agcccccgca cggatccccg aacacggagc gtggacgatg
     2581 ccacaggcca atacctgccc cgagtgcaag cagggcgatg tgatcgcgat ctcgatgaac
     2641 gtgagcggcc gggacctcgc gttctccacc tgccacgcgt gcgaggcgaa gtggtggcat
     2701 cgcgacggcg agcagatcgt gctctccgac gtcatcgaga ccgtcgtccg gacctagcct
     2761 gacgcccgcc gcgcgccgtc cgtaggatgg cgccggtgga cctctccggc atcttcaagg
     2821 cgtacgacgt tcgcggcacg gtccccgatc aactgaacgc cgacgttgtc aggcgcatcg
     2881 gcgcggcatt cgcggactgg agcggagcga acgagatcct ggtcgggcgc gattgccgcc
     2941 tctcttcccc ggagctcgcc gcgaccttga tcgagggtgc cacgtcgcgc ggcgtcgacg
     3001 tcgtcgacct cggactcgcg tcgaccgatc tcttgtactt cgcctccgga tccctcgacc
     3061 ggccgggcgt gatgatcacc gcgagccaca atcccaagga ctacaacgga ttgaagttct
     3121 gtctcgcgcg agcgaagccg gtgggagagg agtcgggcct cggcgagatc cgggcgcgca
     3181 ccgagaaggg tgacgacatc ctcggggcgg gcaacgggtc ggtccgccag cgcgacgtcc
     3241 tgggcgccta cgtcgatcac gttctctcgt tcacggatgt cgatcggatg cgtccgctga
     3301 cggtcgcggt cgacacggcc aatgggatgg gcggactcgt cgtgccggcg gtgatggagc
     3361 ggctgcccgt caccctgcat cacctgtacg cggagctcga cgggacgttc ccgaatcacc
     3421 ccgcggaccc gatcgatccg gcgaaccaac gagacctgaa gcgagccgtc ctcgagcacg
     3481 gtgcggacgt ggggatggcg ttcgacggcg atgcggatcg cgtcttcctg gtggacgagc
     3541 gggcggacga cgtcagcgga tcgatcctca ccgcgctcgt ggcggcggcc atgctggacc
     3601 gggaaccggg tgcgcaggtg gtccacaacc tgatctgttc gtggacggtc cccgaggtga
     3661 tcaaagagca cggtggtgtg ccggtccgaa cccgggtcgg tcactccttc atcaaacagg
     3721 tgatggcgga gacgggcgcg gtgttcgggg gcgagcactc ggggcactac tacttccggg
     3781 acaactacgg cgccgactcc ggactgatcg cgtcggtgat cgcactcggg gagctctcgg
     3841 acgcctcggt cccgttgtcg gggctcctgg ggccgtaccg gcgttacgcg agctccggag
     3901 agatcaattc ccgcgtcgac gatccgctga cgaggatcga ggacatcgcg cgggcgctcc
     3961 gggacggtcg ccaggaccgg ctcgacgggc tgacggtaga gttcgacgac tggtggtgca
     4021 acgtccggcc gtcgaacacg gaaccgctgc tgcggctgaa cgtggaggcc cggacggacg
     4081 agatcctggc tgccagcgcg gcggagctcc tcgcactgat cagagggacg ggagacgccg
     4141 gagggaacgc tccgggaacc tccggagagg gaggaaagga cgagccgtga cgctcgacgc
     4201 agagctgttg gagatcctcg tctgccccaa cgatcggggt gacatcgact accacgagga
     4261 cgagcaggtg atcgtgtgcc gcacgtgcgg ctaccgctac ccggtgcgtg acggcatccc
     4321 ggtgatgctg atcgacgagg ccgagaagcc cggcggtggc ccagggatca agaacggatg
     4381 acgatcgacc tggacgaccc cgaggccgtt cgagcgcacg atcccggggg gatgctggcg
     4441 gccgccgccc agctcgcctc ccactgccgt gagggatatg cgaacggcac gggtgccggt
     4501 gcgctcccgg atctcgtgga cgtccgcaac gtcgtgtact gcgggatggg gggatcggcc
     4561 gtcgccggcg atgtgctgcg atcggtcttc cgcgacaggc tcggcgttcc cgtcgaggtg
     4621 aaccggtcgt cggagctgcc cgagcacgcg ggcccgcatt cgctcgtcgt cgcgtgctcc
     4681 tattcgggca acacctccga gacgctcgcg gcgttccggg aggcgatgaa gcgcggctgt
     4741 cggtcggtcg tgatcacgtc cggcggggcg atcgcggacg aggcgggcga ggcgggcgtg
     4801 ccggtcgtcc gcgtgcccgg cggtatgcag ccgcgtgccg cgctggggtg gctcggatgc
     4861 acggcgctcg gcgcgctgga gaccgcgggg ttgctccccc cgctcgccga ggacatagcc
     4921 gaagttgccg acgaggtcca ggcccgagcc gcgacgtgcg atgcggggag accggttgcg
     4981 aacaaccccg cgaagctcct ggcggaggag atcggcgagc gcatccccgt gatctggggt
     5041 gcggagggca tcggggctgt cgcggcggcc cgctggaaga cgcagatgaa cgag
//
LOCUS       DASUVE010000009         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_944154, whole genome shotgun sequence.
ACCESSION   DASUVE010000009 DASUVE010000000
VERSION     DASUVE010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944154"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VF036_00175"
     CDS             complement(<1..277)
                     /locus_tag="VF036_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263675.1"
                     /translation="MRRHVRTGALAGTVTIFLALVGLIGGFTDVFMIGDEVSFAGLML
                     ILPAFAAGVFAAAPRVEGGERREMSLGEAAVAAAIAAASAGAVFGLAV"
     gene            complement(283..1362)
                     /locus_tag="VF036_00180"
     CDS             complement(283..1362)
                     /locus_tag="VF036_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6263676.1"
                     /translation="MAVARLGRFERLPWRRIILVVLGIVVGGVVVIGAARTLALDPAE
                     GGYSADAWRDFIVQGTAQGAIFAMIALGYSLVYGILRMINFAHGEVFMAGAFASFFFA
                     DAYAKSGFLNRSPILALAIVTLVAVVVSVGTAVLLERLAYRPLRNAPRLVPLITAIGA
                     SLFLMNAFRGFFGPQPYGYPKPDVLAGNITVLGITIQRTQLLVLVVAMICMVALQWFV
                     ATTRTGRSMRAVAQDREIASLMGINVDRVIVVTFAIGGVLAGIAGILFSLTFEQVQFT
                     MGFRPGIAAFTAAVLGGIGSIGGAALGGFVIGLLQAVGPPLILTGFGIPSVVSLRDAF
                     VFLVLVLVLVFRPGGLLGTGEAEKV"
     gene            complement(1465..2787)
                     /locus_tag="VF036_00185"
     CDS             complement(1465..2787)
                     /locus_tag="VF036_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6263677.1"
                     /translation="MKHRIWFKALALVAVLTIVATACADDGGGGGDGGTGAGEVDCAT
                     VEFGCVEVGADEPINLGAAQVISGADASLGQDQVNALNLAIDYLDESFDQTPGTLLDH
                     PVELTVEDELCSAEGGQTAGTALAADTSIVAVVGTSCSSAALGVADTILGDKGITLIS
                     GSNTSPALTDPELHNPFYARTAHNDRIQGAIGAEFALGDDLGATTAATIADESPYTQG
                     LVGAFEANFEAGGGTITGSEQVNSEDTDFKPVLRSLAEQDPEVLYFPIFVAACTLIIR
                     QAQDIMPDAELIVADGCLSSDTLKNAGPAADGVWASSPDLSAFQTNEFYSSEFIPAYQ
                     EAFGTEPLAVFHAHSFDAANVVFAAIEEVAIDNGDGSLSIPRQALRDAIFATNGYEGV
                     TGTISCLDTGDCATDVTIGLFLAPGWPVEGGTDDPAVYTDTKSLDEVL"
     gene            complement(2910..>3554)
                     /gene="trpA"
                     /locus_tag="VF036_00190"
     CDS             complement(2910..>3554)
                     /gene="trpA"
                     /locus_tag="VF036_00190"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX6263678.1"
                     /translation="ADAIEVGIPFSDPVMDGGVIQEASAEALRAGARPGDVLATIREA
                     GIEVPVAIMTYVNPVVRHGISSFLDKVVAAGVAGVIVPDLPVDESSELGRESAARGVD
                     AVLLAAPGTDEERMSEIGRGSRGFVYCVAAYGVTGARDRVEETAGAVVATLRPHTDLP
                     LLVGVGIGTPGQAAEVCAFADGVVVGSALMHRIVGGDVEGAIQLATAFREAIPS"
BASE COUNT          594 a         1275 c         1187 g          498 t
ORIGIN      
        1 taacggccag cccgaacacg gcgccggccg aggcggccgc gatcgcggcc gcgacggcag
       61 cctcgccgag tgacatctct cgtcgttcgc caccctcgac ccgtggcgcg gcggcgaaca
      121 ccccggcggc gaaggccggc aggatcagca tcagtccggc gaacgagacc tcgtccccga
      181 tcatgaacac gtccgtgaag cccccgatca ggccgacgag cgcgaggaag atcgtgacgg
      241 taccggcgag ggctcccgtg cggacgtggc gtcgcacggc tctcagacct tctccgcctc
      301 gccggtgccg agcagccctc ccggccggaa gaccaggacg agcacgagga ccaggaacac
      361 gaacgcgtcc cgcagcgaga cgacgctagg gatcccgaac cccgtgagga tcaacggcgg
      421 cccgaccgcc tggagcaggc cgatcacgaa cccgccgagg gcagcgccgc cgatgctgcc
      481 gatgcccccg aggacggcgg cggtgaacgc cgcgatcccc ggccggaagc ccatcgtgaa
      541 ctgcacctgc tcgaacgtga gcgagaacag gattcccgcg atcccggcga gcacgccgcc
      601 gatcgcgaac gtgacgacga tcacccggtc tacgttgatg cccatcagac tggcgatctc
      661 ccgatcctgg gcgaccgctc gcatcgatcg tcccgtccgc gtcgtcgcca cgaaccactg
      721 cagggcgacc atgcagatca tcgcgacgac gagcacgagc agttgcgtcc gctggatcgt
      781 gatacccaag acggtgatgt tgcccgcgag cacgtctggc ttcgggtacc cgtacggctg
      841 cgggccgaag aaccctcgga acgcgttcat caagaagagc gaagcgccga tcgccgtgat
      901 cagggggacg agccggggag cgttccggag cggacggtag gcgagtcgtt cgagcagaac
      961 cgccgtcccc accgacacga ccaccgcgac gagagtcacg atcgcgagcg caaggatggg
     1021 gctccggttc aggaaaccgc tcttcgcgta ggcgtcggcg aagaagaacg aggcgaaggc
     1081 acccgccatg aagacctcgc cgtgggcgaa gttgatcatc cggaggatcc cgtagacgag
     1141 gctgtacccg agggcgatca tcgcgaagat cgcgccttgc gccgtgcctt gcacgatgaa
     1201 gtcgcgccac gcgtcggccg agtaccctcc ctccgcggga tccagggcga gcgtgcgggc
     1261 cgcgccgatg accaccactc cgccgacgac gatccccagg acgacgagga tgatgcgccg
     1321 ccacggcagt cgctcgaagc gaccgagccg agcgaccgcc acgtgcgggg cctcggtgcc
     1381 cgccagggtt ccccctctca gggaaggggc cacgggcgag ctctcggctc ggcccgcggc
     1441 ccctccgccg tggttcgtgg atcgctagag gacctcgtcc agcgacttcg tgtcggtgta
     1501 cacggcagga tcgtccgtgc cgccctcgac cggccagccg ggagcgagga agagaccgat
     1561 cgtgacgtcc gtcgcgcagt ctcccgtatc gaggcacgag atcgtgcccg tgacgccctc
     1621 gtagccgttc gtggcgaaga tcgcgtcacg cagcgcctgg cgagggatcg agagcgatcc
     1681 gtcgccgttg tcgatcgcga cctcctcgat cgctgcgaac acgacgttcg cggcgtcgaa
     1741 ggagtgcgcg tggaacaccg cgagcggctc ggtcccgaac gcctcctgat acgcagggat
     1801 gaactcactc gagtagaact cgttcgtctg gaacgccgag aggtccggcg acgatgccca
     1861 gacgccatcg gcggcaggtc ccgcgttctt cagggtgtcg ctcgacaggc agccgtcggc
     1921 cacgatcagc tcggcgtcgg gcatgatgtc ctgcgcctgc ctgatgatca acgtgcacgc
     1981 tgccacgaag atcgggaagt acaggacttc gggatcctgt tcggccaacg acctgaggac
     2041 cggcttgaag tcggtgtcct cggagttgac ctgctccgat ccggtgatgg tgccacctcc
     2101 ggcctcgaag ttcgcctcga aggcaccgac gagtccctgg gtgtaggggc tctcgtccgc
     2161 gatcgtcgcc gccgtggtcg caccgaggtc atcgcccagc gcgaactcgg caccgatcgc
     2221 accctggatc cggtcgttgt gagcggtccg ggcgtagaac gggttgtgca gctcggggtc
     2281 cgtcagcgcc gggctcgtgt tggagcccga gatgagcgtg atgcccttgt cgccgaggat
     2341 cgtgtccgcg acgccgagcg ccgcgctcga gcagctcgtc ccgaccaccg cgacgatcga
     2401 ggtgtcggcg gcgagcgccg tgcccgccgt ctgaccgcct tcggcggagc acagctcgtc
     2461 ctcgaccgtg agctcgaccg ggtggtcgag cagcgtgccc ggcgtctggt cgaacgactc
     2521 gtcgaggtag tcgatcgcga gattgagcgc gttcacctgg tcctgaccga gcgaggcatc
     2581 cgccccgctg atcacctgcg cggcgcccag gttgatcggc tcgtccgcgc ctacctcgac
     2641 gcatccgaac tcgacggtgg cacagtcgac ctcgccggcg cccgtccctc cgtcgccgcc
     2701 gccgccgccg tcgtcggcac acgccgtcgc gacgatcgtg agcactgcca ccagggcgag
     2761 cgctttgaac catatgcggt gcttcatgga gccccccttc tcctcggtct cccgagattg
     2821 gatgtgacta tcacatcgcg gccacggttg gccaatcgcc gccgccgtgt ccgcaagggc
     2881 atacgtccac accgggaggc ggagatgcct caggagggga tcgcctcgcg gaacgcggtc
     2941 gccagctgga tcgccccttc gacgtccccg cccacgatcc tgtgcatcag cgcgctcccc
     3001 acgaccacgc cgtccgcgaa cgcacacacc tccgcggcct gaccgggggt gccgatcccg
     3061 acgcccacga gcaacgggag gtccgtgtgg gggcggagcg tggcgaccac cgcccccgcg
     3121 gtctcctcca cgcggtcacg ggcgccggtc acgccgtacg cggccacgca gtagacgaag
     3181 ccgcgcgaac cccggccgat ctccgacatg cgctcctcgt cggtccccgg cgccgccagg
     3241 agcacggcgt cgacgccgcg ggcggcggac tcccgcccga gctcgctcga ctcatcgacg
     3301 ggtaggtccg gaacgatcac gccggcgacc cctgcggcca cgaccttgtc gaggaaagac
     3361 gagatcccgt ggcgcaccac cgggttcacg tacgtcatga tcgcgacggg gacctcgatc
     3421 ccagcctcgc ggatcgtggc gagcacgtca ccgggccgcg cgcccgcacg cagggcctcc
     3481 gccgaggcct cctggatcac gcctccgtcc atcacgggat cggagaaggg gatgccgacc
     3541 tcgatcgcat ccgc
//
LOCUS       DASUVE010000010         8221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_944699, whole genome shotgun sequence.
ACCESSION   DASUVE010000010 DASUVE010000000
VERSION     DASUVE010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8221
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944699"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1719)
                     /locus_tag="VF036_00195"
     CDS             complement(<1..1719)
                     /locus_tag="VF036_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263679.1"
                     /translation="MSQRFRRLVRTGITVVASLTLLVAIWGTQPSRFQDRVREREGEG
                     EELSLQALARRGPRQLPIMVVREKIGESGEAERFGGPAAEAYENRALPRSNIRFKQVK
                     RAIVASVSIPSGRTGLSAPLAAGWQELGPVTPTVPAIATYTDRATQNSGRVTSMAIAP
                     TCEPGDCEIWVGAAGGGVWYAPNALAAEPAWQPLDAGITSNAIGSLAIDPNDPSRVFV
                     GTGEPNGSGDSEAGVGLFTSTGGGPWTLVPASVPIARDRSIGSIAIDPTDSDHYYLGT
                     AVARHGSSSANGGRRTPPNAPTLGLYETTNGGATFDLVFSLDPNPTPASTGNDWFQGG
                     VNRILLDPNDPETVYVALFGYGLWRRSPSIDGTTQFRQVFQTFNPDDTFGDRTEFDLV
                     DVGDTTRIYLGDSSDDLAYSVVWRTDDANVPVADLLTGGTNGGWTLLSSPVNGTDGFG
                     SYFFCHFQCGYDMFVESPPGQPDTVWIGGAMNSVELPVFGGNVRSNGRAVMRSTDAVV
                     SFTDMTNDAQNPPLGMHPDQHAIVFASDPDIAIVGSDGGVIRTDGNYVDDSDECASRP
                     IEGPDLV"
     gene            1875..2342
                     /gene="purE"
                     /locus_tag="VF036_00200"
     CDS             1875..2342
                     /gene="purE"
                     /locus_tag="VF036_00200"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX6263680.1"
                     /translation="MGSRSDWETMKHCCDTLEDLGIRFESKVVSAHRTPALMVEYART
                     AADRGLRVIVAGAGGAAHLPGMTAANTHLPVLGVPIESKALKGMDSLLSIVQMPAGVP
                     VGSLAIGVSGAVNAGLLAAGIVALEDEHVREQLLKFREAQTENVVAHPDPDER"
     gene            2339..3433
                     /locus_tag="VF036_00205"
     CDS             2339..3433
                     /locus_tag="VF036_00205"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX6263681.1"
                     /translation="MTRVGIVGGGQLGMMLAEAGARIGIECVTLDPAPDSPASRVAPS
                     IVAGYDDEAALSELASVSDVVTYEFENVPVSSARFLAERVAVFPPPESLEFAQDRLLE
                     KTLFDEVGLAVAPYAPVSSKEDLGEAMDLVGFPSILKTRRLGYDGKGQARLERPDHAG
                     DAWREVGEVPSILEALVPFDRELSILGARGRDGDAMFYPLVENVHRDGILRTSTHATS
                     VTPALRSNAEELALAVMERLGHVGVLAIELFETGKRLLGNEMAPRVHNSGHWTIEGAA
                     TSQFENHLRAVAGMEIGPTTRTAYSAMVNLIGSEPPDEAFPADPGVHVHRYGKHARPG
                     RKLGHVTVVEKDADTFVQVLREVTRTADNR"
     gene            complement(3497..3625)
                     /locus_tag="VF036_00210"
     CDS             complement(3497..3625)
                     /locus_tag="VF036_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263682.1"
                     /translation="MSTFESHRPHVKAPNQRDGIAWSYSHRWHAQKMISEAQEKQR"
     gene            complement(3722..3847)
                     /locus_tag="VF036_00215"
     CDS             complement(3722..3847)
                     /locus_tag="VF036_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263683.1"
                     /translation="MGDERNILWSEIVASAEANDIAPAEAARNIQEMAEQMGTTA"
     gene            complement(4187..4262)
                     /locus_tag="VF036_00220"
     tRNA            complement(4187..4262)
                     /locus_tag="VF036_00220"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4227..4229),aa:Arg,seq:tct)
     gene            4430..4735
                     /locus_tag="VF036_00225"
     CDS             4430..4735
                     /locus_tag="VF036_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263684.1"
                     /translation="MRDHRRLAIVRHPSVTIEAAAELTDVDVPTVRGWAREGTLVIEQ
                     RGDMEIVLLEEVKALAARRRATKRRGLHDRLADSSTTPAGEVVNIADLHRLARERSE"
     gene            4743..5126
                     /locus_tag="VF036_00230"
     CDS             4743..5126
                     /locus_tag="VF036_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6263685.1"
                     /translation="MSDFGIRADGPHMFSVSGELDIATVPLMNVAIAHAVSQGGPITI
                     DMSFLTFMDSTGVGAILKSAKEMSTGCIVLHGVHDEVSRVIELMGVEGARQNLHVIPC
                     FPPSGDPEVEDASEAPDPSPQQGER"
     gene            5564..5965
                     /locus_tag="VF036_00235"
     CDS             5564..5965
                     /locus_tag="VF036_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263686.1"
                     /translation="MSDVAQRRVTVFNRLFIGLVALGVVVIAVGFVLDTEAAVILVGL
                     AILVASVFTMFLAMPSVLPSGAVMGEWVQLSFVHERFAAELDRFYRRSVEQAAGWNNS
                     PTVKRWVLFVGAALILALLFALCFGGRGTPS"
     gene            6206..6703
                     /locus_tag="VF036_00240"
     CDS             6206..6703
                     /locus_tag="VF036_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263687.1"
                     /translation="MTSKASFTDDEWDRVLRAPMVAGMAITIADPGGPIEISKEMMAT
                     LRAATVPPSQEELLAAVALDIQAMVQQKQNPARNFKPTSAATAGTEILDELRAVDAIV
                     AEKATPEETQAFRGWLVVAAQAAAEAAKEGGFVGFGAVRVSEGEQGMLDQLRSALGQG
                     WASER"
     gene            complement(6713..6783)
                     /locus_tag="VF036_00245"
     tRNA            complement(6713..6783)
                     /locus_tag="VF036_00245"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6749..6751),aa:Gly,seq:tcc)
     gene            6825..7697
                     /locus_tag="VF036_00250"
     CDS             6825..7697
                     /locus_tag="VF036_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX6263688.1"
                     /translation="MEVSFRLVDAFTETPFKGNRLCVVPEIPEGLSDDQMRMLALETN
                     FSETTFVTESHAGGYRMRIFTPDAELPFAGHPTIGTAFTLASEGRTSERTVQTTAVGD
                     VPVEVDLERGFAWMTQQRPVFGEEFVDRAVAAEAVGLQPDDLDPQLPMQVITTGLGPL
                     LVPIKDEATLRRAERYEPGCRRATEASGGECLYLFAIRGDGDVLARMFDASFGVGEDP
                     ATGSAAGPLGVYLASRGLAGMPGEAVVAQGEMVGRPSFLHLDVRPDDGSWRVRVGGGV
                     RIVGEGVFRVASGD"
     gene            complement(7694..8092)
                     /locus_tag="VF036_00255"
     CDS             complement(7694..8092)
                     /locus_tag="VF036_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263689.1"
                     /translation="MRSRSGARSTVTTVLSIALLISIAGFAVLVGLQVRGERPARFDV
                     TQAQGVECPVGEGSPACFSFGVTNLGSKPSLVECIATAPTGLASFLNDMPVYTSSAPF
                     EAGVTEQLIVKVDQGDQGAVSEPALACRAL"
     gene            8131..8204
                     /locus_tag="VF036_00260"
     tRNA            8131..8204
                     /locus_tag="VF036_00260"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8165..8167,aa:Pro,seq:tgg)
BASE COUNT         1290 a         2726 c         2852 g         1353 t
ORIGIN      
        1 cacgaggtcc ggcccttcga tcggcctcga tgcacactcg tcggagtcgt cgacgtagtt
       61 cccgtcggtg cggatcacgc caccgtcgga ccccacgatc gcgatgtcgg gatcggacgc
      121 gaagacgatc gcgtgctggt cggggtgcat gccgagcggc gggttctggg cgtcgttcgt
      181 catgtccgtg aacgagacga ccgcatcggt ggaacgcatc acggcgcgcc cgttcgagcg
      241 gacgttgccc ccgaagaccg ggagctcgac ggagttcatc gcgccgccga tccataccgt
      301 gtcgggctgg cccggcggag attccacgaa catgtcgtaa ccgcactgga agtggcagaa
      361 gaagtacgag ccgaacccgt cggtgccgtt gacggggctc gagagcagcg tccagccgcc
      421 gttcgtcccg cccgtcagca ggtcggccac gggcacgttc gcgtcgtcgg tccgccagac
      481 cacggagtag gccagatcgt cggacgagtc accgaggtag atgcgcgtgg tgtcgccgac
      541 gtcgacgagg tcgaactccg tccggtcgcc gaacgtgtcg tcgggattga acgtctggaa
      601 gacctgtcgg aactgggtgg tgccgtcgat cgacggtgac cggcgccaca gcccgtatcc
      661 gaacagggcg acgtagaccg tctcggggtc gttgggatcc aggaggatcc tgttcacgcc
      721 accctggaac cagtcgttgc ccgtggacgc gggtgtcggg ttcgggtcaa gggagaagac
      781 gaggtcgaac gttgcgccgc cgttggtcgt ctcgtacagg ccgagcgtgg gcgcgttcgg
      841 cggggtcctg cggccgccgt tggccgacga ggagccgtgg cgagcgaccg cggtcccgag
      901 gtagtagtgg tcggagtcgg tcggatcgat cgcgatcgaa ccgatcgaac gatcgcgcgc
      961 gatcggcacg ctggccggca cgagggtcca ggggcctcca ccggtcgagg tgaacaggcc
     1021 gacgcccgct tccgagtcac ccgagccgtt cggctcgccg gtgccgacga agacgcggct
     1081 cgggtcgttg ggatcgatcg cgagggagcc gatcgcattc gaggtgatcc ctgcgtcgag
     1141 cggctgccag gcgggctcgg ctgcgagcgc gttcggcgcg taccacacgc ctccgcccgc
     1201 ggcgcccacc cagatctcgc aatcgcccgg ctcgcacgtc ggtgcgatgg ccatcgacgt
     1261 cacccggccg gagttctggg tcgcacgatc cgtgtacgtc gcgatcgcgg gaaccgtcgg
     1321 cgtcacgggg ccgagctcct gccatccggc ggcgagcggg gcactcagac cggtgcgccc
     1381 cgacgggatc gacaccgagg cgacgatcgc tctcttcacc tgcttgaacc tgatgttcga
     1441 gcgaggcagc gcgcggttct cgtaggcctc ggccgcagga cccccgaagc gctcggcctc
     1501 accgctctcg ccgatcttct cgcgcaccac catgatcgga agctgccggg gaccacgccg
     1561 ggccagcgcc tggaggctca gctcctcccc ctcgccctcc cgctcacgga cacggtcctg
     1621 gaaccggctg ggctgcgtgc cccagatcgc cacgagaagc gtcagcgagg ccacgacggt
     1681 gatgccggtc cggacgagcc tgcggaagcg ctgcgacata agggtccctc ccggggggat
     1741 gtctgggggt gtcaaggtag tgaggcggag gtgactccgc aacctcgacg gacgatcggg
     1801 tcgatggcgg cggctctcgc cgcgagcagc atcggtagac tcgtgccgtg gagaggccgc
     1861 tggtcggcgt gatcatgggc tcccgctccg actgggagac gatgaagcac tgctgcgaca
     1921 cgctcgagga cctcggcatc cggttcgaga gcaaggtggt gtcggcccat cgcacgccgg
     1981 cgctgatggt cgagtacgcg aggaccgcag ccgaccgggg gctccgtgtg atcgtcgccg
     2041 gcgcgggcgg ggcggcgcac ctccccggga tgacggccgc caacacgcac ctgccggtcc
     2101 tcggggtgcc gatcgagtcg aaggcgctga aagggatgga ctcgctcctg tcgatcgtgc
     2161 agatgccggc aggtgtaccg gtcggatcac tcgcgatcgg ggtgagcggc gccgtgaacg
     2221 ccgggctcct cgccgccggg atcgtggcgc tcgaggatga gcacgtccgc gagcagctcc
     2281 tgaagttccg ggaggcgcag accgagaacg tggtcgcgca tcccgatccc gacgaacgat
     2341 gacgcgcgtc gggatcgtcg gcggtggcca gctcggcatg atgcttgccg aggccggagc
     2401 ccggatcggg atcgagtgcg tgaccctcga ccccgctccg gattcgcccg cctcccgggt
     2461 cgctccttcg atcgtggccg gatacgacga cgaagcggcc ctctccgagc tggcctcggt
     2521 ctccgacgtc gtcacgtacg agttcgagaa cgtcccggtc agctccgcac gcttcctcgc
     2581 cgaacgggtc gcggtcttcc ccccgcccga gtccctcgag ttcgcgcagg atcgcctcct
     2641 cgagaaaacc ctgttcgatg aggtggggct ggcggtggcc ccctacgcac cggtctcctc
     2701 caaggaggac ctcggcgaag cgatggacct cgtcgggttc ccgagcatcc tgaagacgcg
     2761 ccgtctcggg tacgacggca aggggcaggc tcggctggag cgcccggacc acgcgggcga
     2821 cgcctggcga gaggtggggg aggtcccctc gatcctggaa gcgctcgtgc cgttcgatcg
     2881 ggagctgtcc atcctcgggg cgcgcggccg cgacggggat gccatgttct atccgctcgt
     2941 cgagaacgtt caccgtgacg ggatcctccg cacctcgacg cacgcgacat ccgtcacgcc
     3001 ggctttgcgg tcgaatgccg aggagctcgc cctcgcggtg atggagcggc tcgggcacgt
     3061 cggggtgctc gcgatcgagc tcttcgaaac ggggaagcgg ctcctgggca acgagatggc
     3121 cccccgcgtg cacaactcag gccactggac gatcgagggt gccgccacgt cgcagttcga
     3181 gaatcacctc cgagcggtcg ccggcatgga gatcggtccg acgacccgaa cggcctacag
     3241 cgcgatggtg aacctgatcg gctcggagcc tccggacgag gcattcccgg ccgatccggg
     3301 cgtgcacgtt caccgctacg gcaagcacgc ccggccgggt cggaagctcg gccacgtgac
     3361 ggtggtcgag aaggatgcag ataccttcgt tcaggtacta agggaggtca cccgaacggc
     3421 cgacaaccgc tgagatggcc tacctcacct gccccgactg ccggatgccc tcaccagttg
     3481 aggacagcgc catctcctac cgctgcttct cctgcgcctc ggagatcatc ttctgcgcgt
     3541 gccatcggtg cgagtacgac caggcgatcc cgtcgcgctg gttcggcgcc ttcacgtgcg
     3601 gccggtgcga ctcgaaggtc gacatccctc gccagcgcta ctacagcacg tcggtgaagg
     3661 cctcacgcgt caagggctac gggtacacct acccacgctt ctagccttcg cccgcttccc
     3721 ctcaggcggt cgttcccatc tgttccgcca tctcctggat gttgcgggcc gcctcggccg
     3781 gcgcgatgtc gttcgcctcg gctgacgcca cgatctcgct ccacaggatg ttccgttcgt
     3841 ctcccatccg gtcttccggc ctcccgcatc cacagttggc acacatcggt ctctcctctc
     3901 gctcgagcct gcgttccgag acacacttgc cccgggagga tcgatccgaa accgcccccc
     3961 gatgcggggg ccatccgcgc gcctacttcg agtctgtgga tggagcgctc gctgcttcgg
     4021 gcttggggca ccgctggtgc ttggctcgtt gatccgtgct cgaccagagg atgcgttcgc
     4081 ccggctggat gtcgccgccg cacctgaggc acttcgtcgc gaagctcgcc gttcgctgga
     4141 agaccgccac cggatccgcc cctttctctt cgccggttcg tccggctggc gcgccgggca
     4201 ggattcgaac ctgcgacctg aggattagaa gtcccctgct ctatcccctg agctaccggc
     4261 gctcgtccgg agcgtacctt catgcgggtg ctcccagaga ggtctgggat cggagcggtc
     4321 cgcgccatcg gtcttaagta caacggcgac tcgacccgag ccatccgccg gggaaccgta
     4381 gcccgagcgg ccgatgcggc gactcggtca tcgaccctac ggtcgcacga tgagagacca
     4441 cagacgcctt gccatcgtcc gccatccctc cgtaacgatc gaagccgccg ccgagctgac
     4501 cgatgtcgac gttcccacag tccgcggatg ggcacgcgag ggaacgcttg tcatcgagca
     4561 gcgcggcgat atggagatcg tcctgctgga ggaggtgaaa gcgctcgcag cgaggcggcg
     4621 agcgacgaaa cgcagaggac tccatgaccg actggctgac agcagcacaa cgccggcggg
     4681 ggaggtcgtg aacatcgctg accttcacag gttggcgaga gagcgatcgg agtagcccaa
     4741 cgatgtcgga cttcgggatc agggccgacg gtccgcacat gttctcggtg agcggggaac
     4801 tggatatcgc gaccgtgcct ctcatgaacg tggcgatcgc acacgccgtg tcgcagggcg
     4861 gacccatcac gatcgacatg agcttcctca ccttcatgga ctccacggga gtgggggcga
     4921 tcctcaagtc ggcgaaggag atgtcgaccg ggtgcatcgt tcttcacggc gttcatgacg
     4981 aggtcagcag ggtgatcgaa ctcatgggcg ttgagggggc acggcagaat ctccatgtca
     5041 tcccttgctt cccccccagc ggggatccag aggtcgaaga tgcgagcgag gcgcctgatc
     5101 cgtcgcccca acaaggagag cggtgacgag gatcggactc gcactacagt gccgatgaca
     5161 tctccctccg gaccgagcgc atcgagccgg cacgtgacgt cggcggttcg ctcctcgtta
     5221 gttgccaggt tgcccttgcc aggccggccc cgactcgccg gccgggtccg gccccctcct
     5281 cgccgtgggc gttcatccgg ctatcccggg gtgtaacgtc gcccgcgatg gacgtcgggc
     5341 agtgtgaggt cgactagttg gccgtggtac acgcacgtgt ggccgatctc gaagcgggca
     5401 cgctgcctcc ggtgtgcgcc aagacgggcg tcccgacgga cggatgggcc aagatccgat
     5461 tctcttcggc ccgacgtgga cctggatcct gttgcttttc gggatcttcc ctttcctgat
     5521 cgctcagtac ttcgccacgg tccacgttga agggatcgtt cccatgtccg atgttgcgca
     5581 gcggcgcgtg acggtcttca accggctctt catcgggctc gtcgccctcg gcgtggtcgt
     5641 catcgcggtt ggattcgtgc tcgacaccga ggcggctgtc atcctggtgg gactggcgat
     5701 cctggtcgcg tccgtcttca cgatgtttct ggcgatgcct tccgtgctac cgagcggtgc
     5761 ggtcatgggc gagtgggtgc agttgtcctt cgttcacgag cgcttcgccg cggagctaga
     5821 ccgtttctac aggcgctccg tcgagcaagc cgcaggctgg aacaacagcc ccaccgtcaa
     5881 gaggtgggtg ctgttcgtcg gggccgcgct gatcctcgct ctgctgttcg cactctgctt
     5941 cggcggacgc ggaactccgt cttgactcgg gactgacctc ttcggaggac ggcgtgggcg
     6001 gtgtctgggt cgcagggcgg tcccacgggt ccggcctcca tcgaaccacc ttgccagcga
     6061 tccgcaggcc acggggcatc tgagccccat cggctggtcg accgagggcc tccggcgtct
     6121 ccaggtaggg cgggcgccgc cgtgcgccac gagtaggctg gcatccgggc gatgaagggg
     6181 atgaggggca tgagggggga ggtccatgac gtcgaaagca tcgttcactg acgacgagtg
     6241 ggatcgcgtg ctccgggcgc cgatggtggc ggggatggcg atcacgatcg ctgaccccgg
     6301 cggtccgatc gagatctcga aggagatgat ggcgacgctc cgcgccgcca ccgttccccc
     6361 cagccaggaa gagttgctcg ccgcggtggc cctcgacatc caggccatgg tccaacagaa
     6421 gcagaacccc gcgcggaact tcaagccgac gagtgcggca accgccggca ccgagatcct
     6481 cgacgaactg cgtgccgtcg acgcgatcgt cgccgagaag gcaacgcctg aagagaccca
     6541 ggcgtttcgc ggttggctcg tcgtggcagc gcaggcggct gctgaggccg cgaaggaagg
     6601 cgggttcgtg ggtttcggcg cggtgcgagt cagcgaaggc gagcaaggca tgctggatca
     6661 gctccgaagc gctctgggac aagggtgggc ctcagagcga tgacccctgc agagcgggtg
     6721 acgggaatcg aacccgcacc accagcttgg aaggctggag ctctaccatt gagctacacc
     6781 cgcgcggagc cagcctagca acacccggct gggatacttc gccggtggag gtctccttcc
     6841 ggctcgtcga cgcgttcacc gagacaccct tcaagggcaa ccggctgtgc gtcgtgcccg
     6901 agatcccgga gggcctctcc gacgatcaga tgcggatgct cgcgctcgag acgaacttct
     6961 cggagacgac gttcgtgacc gaatcgcacg ccggcggcta tcggatgcgg atcttcactc
     7021 cggacgccga gctgcccttc gccgggcatc ccacgatcgg gacggcgttc accctggcga
     7081 gcgagggtcg gacgtccgag cgaacggttc agacgaccgc ggtcggcgac gtgccggtcg
     7141 aggtcgacct cgaacgcggg ttcgcctgga tgacccagca gcggccggtg ttcggcgagg
     7201 aattcgtcga tcgtgcggtc gccgccgagg cggtcggtct gcaacccgac gacctcgatc
     7261 cccagctccc gatgcaggtg atcacgaccg gactcggccc actgctcgtg ccgatcaagg
     7321 acgaggccac gttgcgacga gcggaacgct acgaaccggg atgccggcgc gcgaccgagg
     7381 cttccggtgg cgagtgcctc tacctgttcg cgatccgagg ggacggcgac gtgctcgcgc
     7441 ggatgttcga cgcgtccttc ggcgtcggag aggatccggc gaccggctcg gccgccggtc
     7501 cgctcggcgt ctacctggcg tcgcgcggcc tcgcggggat gccgggcgag gccgtggtcg
     7561 cccagggcga gatggtgggg cggccgagct tcctgcacct cgacgtccgg cccgacgatg
     7621 gctcgtggag ggtgcgggtg ggcggcggcg tccggatcgt cggtgagggc gtcttccgcg
     7681 tggcgagcgg ggactagagg gccctgcagg ccaacgccgg ttcggacacg gcgccctggt
     7741 ccccttgatc gaccttgacg atcagctgct cggtcacccc ggcctcgaac ggggccgagc
     7801 tcgtgtagac cggcatgtcg ttcaggaagc tggcgaggcc cgtcggggcc gtcgcgatgc
     7861 attccacgag ggacggcttg ctgccgaggt tcgtcacgcc gaagctgaag cacgccgggc
     7921 tgccttcgcc gaccgggcac tccacacctt gggcttgcgt gacgtcgaag cgagccgggc
     7981 gttcaccgcg aacctgcaag cccaccagga ccgcgaaccc cgcgatggag atgagcaacg
     8041 cgatcgagag gacggtggtg acggtgctcc gggccccgga ccgcgaacgc atgccccagg
     8101 gtaccgggac gtagaatcgc tgcacaagag cggggcgtag cgcagcctgg tagcgcacgt
     8161 gctttgggag cacgaggtcg cgggttcgaa tcccgccgcc ccgaccgacg gctagcggtc
     8221 c
//
LOCUS       DASUVE010000011         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_945377, whole genome shotgun sequence.
ACCESSION   DASUVE010000011 DASUVE010000000
VERSION     DASUVE010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945377"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VF036_00265"
     CDS             <1..484
                     /locus_tag="VF036_00265"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX6263690.1"
                     /translation="VGCFGPSRPNGGGTVADQRSDDISVVGRDARIEGTLTSAGSLRI
                     HGRLTGAITVEGEVSVAAGSEVRADINAQTISLAGRVKGNLTAPGAVVLPEQSRVEGD
                     VRAQNVTVHGAVTGDVVAEQGVRIGGQARVEGDITCKTLAIEEGAYFAGRSNMSDTGR
                     "
     gene            601..1500
                     /locus_tag="VF036_00270"
     CDS             601..1500
                     /locus_tag="VF036_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263691.1"
                     /translation="MRRMTLAWSVVLSLMLSIVLAPTANAAFPGGNGETAFGRTMNGQ
                     NDIWIVRPGVTGTNRLTHTAHRNEGMPDYNAAGTLIAFSRCGEGDFPNCDIWSMNADG
                     TAEVRLTFTPDVQETWPTWSPDGMQIAFTSDANDPSQDIWVMDANGANQTQLTLTNGF
                     DAFPEWSPDGTKIAFTSDRAAPDDIWVIDADGSNPTRLTAGPRVDERPDWSPNGVRIA
                     FSRNGNIWKMRSDGSHEVKLTFNTRHEFAPTFSPNGKRIAFDRRSKDGLRFNIWTIRA
                     DGSGVAVHRTFARFDFAPDWQPL"
     gene            1507..>2599
                     /locus_tag="VF036_00275"
     CDS             1507..>2599
                     /locus_tag="VF036_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6263692.1"
                     /translation="MDRQTRYAKTADGVHIAYQVLGDGPVDVVFVMGWVTNIEAMWDE
                     PDFARFLDRLATFSRLILFDKRGVGLSDRVPEDRLPDLETRMDDVRAVMDAVGSERAV
                     VFGVSEGGPMSMLFAATYPERTVALVLYGTVADFTARAPAYKGDPAAYLARTEEMWGS
                     LEFARGEIAGWGAPGHESDERLVEWFASYMRKSASPGAAVALERMNRQINATHALSSI
                     HVPTLVIDKDGDLDFPIDQVRDMASRIAGARLVELSGSEHLPWVGAHEPILDEVERFV
                     VALGEVEAEFDRALATVLFTDVAGSTEKAAELGDRRWKELVEEHHRLVRGQLARYRGV
                     EVDTAGDGFFATFDGPARAVRCATSVVHAV"
BASE COUNT          425 a          874 c          915 g          385 t
ORIGIN      
        1 cgtaggctgc ttcggaccca gccgaccgaa cggaggaggc accgtggccg atcagcggag
       61 cgacgacatc tcggtcgtgg gacgcgatgc acggatcgag ggcacgctca cctcggcggg
      121 ctccctccgg atccacggcc gcctcaccgg cgcgatcacg gtggagggag aggtgtcggt
      181 cgcggccggc agcgaggtgc gggccgacat caacgcccag acgatcagcc tcgccggacg
      241 cgtcaagggc aacctcaccg cgccgggcgc ggtggtgctg ccggagcaga gccgcgtcga
      301 gggggacgtg cgcgcccaga acgtgacggt gcacggagcc gtcaccggtg acgtcgtggc
      361 ggagcagggc gtccgcatcg gcggccaggc gcgcgtcgag ggcgacatca catgcaagac
      421 gctcgcgatc gaagaaggcg cctacttcgc cggccgctcg aacatgtccg acacgggccg
      481 gtaggaacga tcggcggctg acggcccacg gccctccgca ccgcactacc gggtcggcta
      541 cgctcggcgg atcgggtctg gtcaaccgga ccgccccggg aaagtacgga ggtggtgctc
      601 gtgcggcgca tgacgctcgc gtggtccgtg gtcctgtcac tgatgctgtc gatcgtcctt
      661 gcgcccacgg cgaacgccgc gttccccggc gggaacgggg agacggcgtt cggtcgaacg
      721 atgaacggtc agaacgacat ctggatcgtc cgccccgggg tcaccggcac gaaccggctg
      781 acgcacaccg cgcaccggaa cgagggcatg cccgattaca acgccgccgg gacgctgatc
      841 gccttctccc gttgcgggga gggcgacttc ccgaactgcg acatctggtc gatgaacgcc
      901 gacgggacgg ccgaggttcg gctcacgttc accccggacg ttcaggagac atggccgacc
      961 tggtcgcccg atgggatgca gatcgcgttc acgtctgatg ccaacgaccc gtcgcaggac
     1021 atctgggtga tggacgcgaa cggcgcgaac cagacccagc tcacgctcac gaacgggttc
     1081 gacgcgttcc ccgagtggtc gccggacggc acgaagatcg cgttcacgag cgaccgcgca
     1141 gcccccgacg acatctgggt gatcgacgcc gacggctcca acccgacccg cctcactgcc
     1201 ggacctcggg tcgacgagcg cccggactgg tcgccgaacg gggtgcggat cgcgttctcc
     1261 cggaacggga acatctggaa gatgcgctcg gacgggagcc acgaggtcaa gctcacgttc
     1321 aacactcggc acgagttcgc cccgacgttc tcgccgaacg ggaagcgcat cgcgttcgat
     1381 cgacggtcca aggacgggtt gcggttcaac atctggacca tccgcgcgga cgggtccgga
     1441 gtcgccgtcc atcgcacgtt cgcgaggttc gacttcgctc ccgactggca accgctctag
     1501 ttccacgtgg atcggcagac ccgctacgcg aagaccgccg acggggtcca catcgcctat
     1561 caggtcctcg gcgatggacc cgtcgacgtg gtcttcgtga tggggtgggt gaccaacatc
     1621 gaggcgatgt gggacgaacc cgacttcgcc cggttcctgg atcgcctcgc gacgttctcc
     1681 cgcttgatcc tgttcgacaa gcgcggcgtc ggcctctccg accgcgtgcc cgaggaccgg
     1741 ctccccgacc tcgagacccg gatggacgac gtacgcgcgg tgatggatgc cgtcgggtcg
     1801 gaacgggcgg tcgtcttcgg ggtctccgag ggcggcccga tgtcgatgct cttcgccgcg
     1861 acctatcccg agcgcacggt cgccctcgtc ctctacggca cggtcgccga cttcacggcc
     1921 cgagcgcccg cctacaaggg cgacccggcc gcctacctcg cgcgcacgga ggagatgtgg
     1981 gggagtctcg agttcgcgcg gggcgagatc gcaggctggg gtgcgcccgg tcacgaatcc
     2041 gacgaacggt tggtcgaatg gttcgcgtcg tacatgcgga agtcggccag cccgggtgcc
     2101 gcggtcgccc tcgagcggat gaatcgccag atcaacgcca cgcacgcgct ctcgtcgatc
     2161 catgtgccca cgctggtgat cgacaaggat ggagacctcg acttccccat cgaccaggtg
     2221 cgggacatgg cgagtcgcat cgccggagcc aggctcgtgg agctctcggg gagcgagcac
     2281 ctgccctggg tcggcgctca tgaaccgatc ctcgacgagg tggagcgatt cgtggtcgct
     2341 ctgggcgagg tggaggcgga gttcgaccga gcgttggcga ccgtgctgtt caccgacgtc
     2401 gccggatcca ccgagaaggc cgccgagctc ggtgatcgac gctggaagga gctggtcgag
     2461 gagcaccatc gtctggtgcg gggccagctc gcgcgctacc gcggcgtgga ggtcgacacg
     2521 gcgggggacg gtttcttcgc cacgttcgac ggccccgcgc gagccgtcag gtgcgccaca
     2581 tcggtcgtcc acgcggtcg
//
LOCUS       DASUVE010000012         2347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1322086, whole genome shotgun sequence.
ACCESSION   DASUVE010000012 DASUVE010000000
VERSION     DASUVE010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322086"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VF036_00280"
     CDS             complement(<1..477)
                     /locus_tag="VF036_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263693.1"
                     /translation="MQAETLADLPPTLLPYPFTEPAPEPVATEIDGTYMLILTLDELG
                     GASHALPFPCRRCLPYARDPGVMTLIFFRGAYFVDHQMSSFHSTGHYEVEGDRLTLFN
                     DPNCSRERGTYTWALERGSLHLETIADTCAYEGERATDLATEDWTRIPVCRREIAHL"
     gene            515..655
                     /locus_tag="VF036_00285"
     CDS             515..655
                     /locus_tag="VF036_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263694.1"
                     /translation="MSIGAGAGAASPQVSAIAASSMETAATADGRSLFLVDCDPRVTG
                     SG"
     gene            688..1398
                     /gene="hxpB"
                     /locus_tag="VF036_00290"
     CDS             688..1398
                     /gene="hxpB"
                     /locus_tag="VF036_00290"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: protein motif:HMM:NF008087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HEX6263695.1"
                     /translation="MDADTRPIEAVVFDMDGVLVDSEPMWRAVEREVFASVGIELTDE
                     DLYPTMGVRIADVVDRWYQRYPWSEPSREQVAATIVDGVATAVRARGLLLDGARDAVD
                     HVRSLGLGVALASSSPMALILAVLSLDGLAARFDVVVSGEDEELGKPDPAVFLSAARR
                     LEVPPERCLAVEDSINGVRAAKAAGMLCVAVPAVGGDAEIGTEADLLLGSIGELDDRV
                     WARLGAVPLASRTMRGDG"
     gene            1395..2225
                     /locus_tag="VF036_00295"
     CDS             1395..2225
                     /locus_tag="VF036_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6263696.1"
                     /translation="MNEIPTITLANGVAMPALGLGTSPMGDRQAEETVSAALRAGYRL
                     IDTAENYRNETGVGRGIRASGVDRDEVFVTTKLNARWHGEAEAQQAWANSAERLGLDR
                     IDLYLIHWPNPGKDRYVEAWRGLLRLLEDGKVRAIGVSNFKPPHLDQLLDETGVTPHV
                     NQIQLDPRIGRAEERAYHEAHGIVTESWSPIGAGGDLLRAPTIVEVARRRSKTPAQVV
                     LRWHVQLGLLPIPKTSKAERLPENRDVFDFQLDETDMDELAALDRGDEGVVDADRFGH
                     "
BASE COUNT          343 a          767 c          885 g          352 t
ORIGIN      
        1 caggtgggcg atctcgcgcc ggcacaccgg gatccgcgtc cagtcctccg tcgcgagatc
       61 ggtcgcccgc tccccctcgt acgcgcacgt gtcggcgatg gtctcgaggt ggagcgagcc
      121 tctctccagc gcccacgtgt acgttccgcg ctcccgggag cagttcgggt cgttgaacag
      181 cgtgagccgg tcgccctcca cctcgtagtg cccggtcgaa tggaacgagg acatctggtg
      241 gtccacgaag tacgcacctc ggaagaagat cagggtcatc accccggggt cgcgcgcgta
      301 cgggagacac cgtcggcacg ggaaggggag cgcatggctc gcgcccccga gttcgtcgag
      361 cgtgaggatc agcatgtacg tcccgtcgat ctcggtggcg acgggctccg gcgccggctc
      421 cgtgaacggg taggggagca gtgtgggggg caggtccgcg agcgtctccg cctgcaacac
      481 ctgaccctcg ggctggggtg acggcgaggg ggaggtgtcg atcggggcgg gcgccggcgc
      541 ggcgtcgccc caggtcagcg cgatcgccgc gagcagcatg gagacggcgg ccaccgcgga
      601 cggcaggagt ctgttcctcg tcgactgcga tcctcgggtc acgggcagtg ggtgatgata
      661 cgcggcgcgt ccgtagcatc cgatgccgtg gatgcggaca cgcgaccgat cgaggccgtc
      721 gtcttcgata tggacggcgt gctggtcgac tcggagccga tgtggcgggc ggtggagcgc
      781 gaggtgttcg cgagcgtcgg catcgagctc acggacgagg atctgtatcc gacgatgggc
      841 gttcggatcg ccgacgtcgt ggaccgttgg tatcagcggt acccgtggtc cgaacccagt
      901 cgtgagcagg tggcggcgac gatcgtcgac ggggtcgcca ccgccgtccg tgcgcgtggc
      961 ctcttgctcg acggggcgcg cgacgcggtc gaccatgttc ggtcgctcgg gctgggggtc
     1021 gccctcgcgt cgtcgtctcc gatggccctg atcctggcgg tgctctccct cgacgggctc
     1081 gccgcgcggt tcgacgtcgt ggtctccggc gaggacgagg agctcggcaa gcccgatccc
     1141 gccgtcttcc tgtccgcggc tcggcgtctc gaggtcccgc cggaacggtg tctcgccgtg
     1201 gaggactcga tcaacggcgt ccgggccgcg aaagccgcag ggatgctctg cgtcgcggtg
     1261 ccggccgtcg ggggcgacgc cgagatcggg accgaggcgg atctcctcct cggatcgatc
     1321 ggcgagctcg acgaccgggt gtgggcgcgc ctcggtgcgg tcccgctagc atcccggacg
     1381 atgcgaggcg acggatgaac gagataccca cgatcacgct cgccaacggg gtggcgatgc
     1441 ccgcgctggg gctgggcacc tcgccgatgg gcgaccggca ggccgaggag acggtctcgg
     1501 ccgcgcttcg agcgggatac cggctgatcg acaccgccga gaactaccgg aacgagaccg
     1561 gcgtgggccg ggggatccgg gcgagcggcg tcgatcgcga cgaggtgttc gtcaccacga
     1621 agctcaacgc gcgctggcac ggcgaggcgg aggcacagca ggcgtgggcg aacagcgcgg
     1681 agcgactcgg cctcgaccgc atcgacctgt acctgatcca ctggcccaat cccgggaagg
     1741 accggtacgt ggaagcgtgg cgcgggctcc tccggctcct cgaggacggg aaggtccgcg
     1801 cgatcggcgt ctcgaacttc aagcctccgc acctggacca gctgctcgac gagacgggcg
     1861 tgacgccgca cgtgaatcag atccagctcg acccgaggat cggccgagcc gaggagcggg
     1921 cgtaccacga ggcgcacggc atcgtgacgg agagctggtc gccgatcggc gcgggcggtg
     1981 atctcctccg cgccccgacg atcgtcgagg tggcccgacg tcgctcgaag acgccggcgc
     2041 aggtcgtgct ccggtggcac gtgcagctcg ggctcctccc gatccccaag acctcgaagg
     2101 ccgagcggct gcccgagaac agagacgtct tcgacttcca gttggacgag accgacatgg
     2161 atgagctcgc ggccctcgat cgaggggacg aaggggtcgt ggacgccgac cgcttcggtc
     2221 actgagcggt cgctccgtcg gtacgctggg tccatggcgc ggtcggtgtg gcgcgggacg
     2281 ctcagcttcg ggctcgtcgc gatcccggtg tccctctacc ccgcgaccga ggcgaaggac
     2341 gtgcggt
//
LOCUS       DASUVE010000013         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1510807, whole genome shotgun sequence.
ACCESSION   DASUVE010000013 DASUVE010000000
VERSION     DASUVE010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510807"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="VF036_00300"
     CDS             <1..1203
                     /locus_tag="VF036_00300"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX6263697.1"
                     /translation="EVLRGAIDAGAAPPRRRLQLRASRASALLGYPVTAQDATTALGS
                     IGVRATAVDEDVLDVEVPGFRPDLEVEVDAIEEIVRVHGYDRLPSTVPGVRVSGGEQD
                     SYVLRRRVRALLMRCGLREAASLSFASPADVELMGHDAAIAVANPPSAAQPLLRTSLI
                     PGLLTAVRRNLDLGARSVKLFEVGHVFRLGEPVDEREHVAVVLGGLVGEGLHAEGREQ
                     DVLDVKGVVEFLLDGLRVGWTLEVPAEPPLHPGRSGIVTVAGRRAGVFGELHPLDASR
                     LGLTGRVAVAELDVSSISAEPVAVTFRDVPRFPPVRRDLAFVVPAGVSAGMLQAALDD
                     AGGSLLDRSTLFDVFAGGAIPEGRTSLAFALEFRAPDRTLTDDEVEPVVIAIVDRVRS
                     EFGAELRA"
     gene            1220..2167
                     /gene="argF"
                     /locus_tag="VF036_00305"
     CDS             1220..2167
                     /gene="argF"
                     /locus_tag="VF036_00305"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX6263698.1"
                     /translation="MSAVPLNSGVMRHYLSVDDLSAAELPELIDLGAALKADRSAHAR
                     TLAGRSIALIFEKPSTRTRVSFEVGVEQLGAHAVVLSAAELQLGRGETIQDTGRVLSR
                     YVDAIVLRTFEQERLEILSTTATVPVINALSDFEHPCQALADLLTIREHLGDLHGRVL
                     TYLGDGNNVAHSLLLAGTKAGMAVRVATPPGFEPIPQVVHRAEEIAAETGGSVELTHD
                     PEGAAIGAHILSTDVWTSMGQDLEADERSLVFPSYQLGQKLVDLADPDVIVLHCLPAH
                     RGQEITDEVIDGPRSVVWDQAENRLHTQKALLLRLLGVA"
     gene            2172..>3344
                     /locus_tag="VF036_00310"
     CDS             2172..>3344
                     /locus_tag="VF036_00310"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX6263699.1"
                     /translation="MPRANDAIEAMLIEYADLLSILFEDPYKPRAYEKAARAVGGYPV
                     DLEGKDLPEILKVPSVGRSIGEKIQEFLTTGVLAELEELRERIPPGVRGLMSIPGLGP
                     KKAMVLHQELGVGSVDELVAAIEQDRVAALPRFGAKTQENIARGIRQMQAAGGRVKID
                     VALGVAGTFLELLGSVPEARRVAFAGSLRRMKETIGDVDLLVAGSDAPPVMEAFVTHP
                     SVARVLARGDTKTSVVTDLGLQIDLRVIAEEAWGAAMIYFTGSKPHNIRIREMAVRRG
                     LKLSEYGLFDAKSGDLLAAESEERVYERLGMQYVEPTLREDRGEVEAALAGELPVVVT
                     ERQLKGDLHTHTNLTDGLASLAEMVARAAGLGYAYFAITDHAPNLYMQRMTEAKILA"
BASE COUNT          476 a         1178 c         1165 g          525 t
ORIGIN      
        1 gaggtccttc gcggggcgat cgatgcgggg gcggctccgc cgcggcgccg gttgcagctc
       61 cgcgccagcc gcgcctcggc cctgctcgga tacccggtga cggcacagga cgcgaccacc
      121 gccctcggct cgatcggcgt ccgggccacc gcggtcgacg aggacgtcct ggacgtcgag
      181 gtgccggggt tccgtccgga cctcgaggtc gaggtcgacg ccatcgagga gatcgtccgc
      241 gtgcacgggt acgatcgcct gccttcgacc gttcccggtg tgcgcgtgtc ggggggtgag
      301 caggacagct acgtgctccg ccgccgggtg cgtgccctgc tgatgcgctg cgggctccgc
      361 gaggccgcgt ctctctcctt cgcatcgccc gccgatgtcg agctgatggg ccacgacgcg
      421 gcgatcgccg tggccaaccc accctcggcc gcgcagccgc tcttgcggac gagcctcatc
      481 ccgggcctcc tcaccgcggt tcggcggaac ctcgatctcg gcgcccggtc cgtcaagctg
      541 ttcgaggtgg gacacgtctt ccgtctcggc gagccggtcg acgaacgaga gcacgtcgcc
      601 gtcgtcctcg gggggctcgt cggagagggc ctccacgccg agggtcgaga gcaggacgtc
      661 ctcgacgtga aaggagtggt cgagttcctc ctggacgggc tccgcgtcgg atggacactg
      721 gaggtccccg ccgagcctcc gctccacccc ggtcggtcgg gtatcgtcac ggtcgccggg
      781 aggcgcgccg gtgtgttcgg ggagctccac ccgctcgacg cgtcgcgcct cggactcacc
      841 ggtcgggtgg ccgtcgccga gctggacgtc tcctcgatct ccgccgagcc agtcgccgtg
      901 acgttccgcg acgtcccccg attccccccg gttcgtaggg acctcgcgtt cgtggtgccc
      961 gccggcgtgt cggccggcat gctccaggcg gccctcgacg acgcaggggg atcgctcctc
     1021 gatcgatcca cgctcttcga cgtcttcgcg ggcggcgcga tcccggaggg ccgcacgagc
     1081 ctggccttcg ctctcgagtt ccgggcgccc gaccggacgc tcaccgacga cgaggtcgag
     1141 ccggtggtga tcgcgatcgt cgaccgagtg cgttcggagt tcggggcgga gctccgggcc
     1201 tgaggttcgc atcacaccca tgtcggcggt cccattaaac tcgggcgtca tgcgtcacta
     1261 cctgagcgtc gacgacctct ccgctgccga gctccccgag ctcatcgacc tcggcgcggc
     1321 actgaaggca gatcggagcg cgcacgcccg aacgctggcc ggccgatcga tcgcgttgat
     1381 cttcgagaag ccctccaccc gcacgcgtgt ctcgttcgag gtcggggtgg aacagctcgg
     1441 cgcgcacgcc gtcgtgctgt ccgcggcgga gctccagttg ggtcgcggtg agacgatcca
     1501 ggacaccggt cgtgtcctgt cgcgctacgt cgacgcgatc gtgctccgga ccttcgagca
     1561 ggagcgcctg gagatcctct ccaccacggc gacggtcccg gtgatcaacg ccctctcgga
     1621 cttcgagcac ccctgccagg cgctggccga cctcctcacg atccgcgagc atctcggcga
     1681 cctccacggg cgagtcctga cgtacctcgg ggacggcaac aacgtcgcgc attccctgct
     1741 cctggcgggg acgaaggccg gcatggccgt ccgcgtggcg acaccgccgg gcttcgagcc
     1801 gatcccccag gtggtgcacc gggccgagga gatcgctgcc gagacgggtg gctccgtcga
     1861 gctcacgcac gatcccgagg gtgcggcgat cggtgcccat atcctgtcca ccgacgtctg
     1921 gacgagcatg ggtcaggacc tcgaggcgga cgagcgctcc ctcgtcttcc cgagctacca
     1981 gctgggtcag aagctcgtgg acctcgcaga ccccgacgtg atcgtcctcc actgcctgcc
     2041 cgcgcaccgc gggcaggaga tcaccgacga ggtgatcgac gggccgcggt ccgtggtctg
     2101 ggatcaggcc gagaaccggc tccacacgca gaaggcgctg ctgctccgcc tcctcggcgt
     2161 agcctgaggc gatgccccgg gcgaacgacg ccatcgaggc gatgctcatc gagtacgccg
     2221 accttctgtc gatcctgttc gaggatccct acaagccgcg cgcctacgag aaggccgcgc
     2281 gggccgtcgg cggctatccg gtcgacctgg aaggcaagga tctccccgag atcctgaagg
     2341 tcccctcggt cgggcgctcg atcggcgaga agatccagga gttcttgacg acgggtgtgc
     2401 tcgccgagct cgaggagctc cgggagcgga tcccgcccgg cgtccgtggc ctgatgtcga
     2461 tccccgggct cggcccgaag aaagccatgg tgctccacca ggagctcggt gtgggctccg
     2521 tggacgagct cgtggccgcg atcgaacagg atcgagtcgc cgcgctgccg cggttcgggg
     2581 cgaagacgca ggagaacatc gcccgtggga tccggcagat gcaggccgcc ggcggccgcg
     2641 tcaagatcga cgtcgcgctc ggtgtcgcgg ggacattcct cgagctcctc ggctccgtgc
     2701 ccgaagcccg acgcgtcgcg ttcgccggtt ccctccggcg gatgaaggaa acgatcggtg
     2761 atgtggatct cctcgtcgcc ggatccgacg ccccgccagt gatggaggcg ttcgtcaccc
     2821 acccctcggt cgcccgcgtc ctcgcgaggg gggacaccaa gacctccgtc gtgaccgatc
     2881 tcggcctcca gatcgatctc cgcgtcatcg cggaggaggc gtggggggcg gcgatgatct
     2941 acttcaccgg ctccaagccg cacaacatcc ggatccggga gatggccgtt cgccgggggt
     3001 tgaagctcag cgagtacggg ttgttcgacg cgaagtcggg cgatctgctc gcggccgagt
     3061 ccgaggagcg cgtgtacgag cgcctcggca tgcagtacgt cgaacccacc ctgcgcgagg
     3121 atcgagggga ggtcgaggcc gcgctcgccg gcgagctccc cgtcgtggtg acggagcggc
     3181 agctgaaggg cgatctccac acgcacacga acctgaccga cggcctcgcg agcctggcgg
     3241 agatggtcgc ccgcgctgcc gggctcgggt acgcgtactt cgcgatcacg gatcacgcgc
     3301 ccaacctgta catgcagcgg atgaccgagg cgaagatcct ggcg
//
LOCUS       DASUVE010000014         5831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_380861, whole genome shotgun sequence.
ACCESSION   DASUVE010000014 DASUVE010000000
VERSION     DASUVE010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380861"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1433
                     /locus_tag="VF036_00315"
     CDS             120..1433
                     /locus_tag="VF036_00315"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX6263700.1"
                     /translation="MSAREANTGEGRTVRIGLLGSGTVGAAVIRLLHEHGDDVARRAG
                     VHLEVVRVAVRDPGRDRGLPLAADRFTDDAGAVVDDPTIDIICELMGGVEPARGLLVR
                     ALASGKSVVTANKELLASHGEELYTAVEEAGGDLYFEAAVAGGIPLIRPLRESLAGER
                     VDRILGIVNGTTNFILTQMSEHGWTADEAVAEAQRLGFAEADPTADVEGYDAAAKCAI
                     LASVAFGARVVANDVYREGISAVTPQDFRDAARLGYVIKLLAIAELDDEAIAVRVHPA
                     MIPDVHPLASVRGASNAVFVEGPKIGQLMFYGAGAGGDPTATAVVGDLVSVARNLLQG
                     ARGPGPGSTNARRVRPMDDTHDQYYLKLRVDDRPGVLAEIADRFGRNGISLERVWQEG
                     TGEEATLSFITHRAQEGAFQKTMRELRELDAVHAVASVLRVEGEE"
     gene            1433..2494
                     /gene="thrC"
                     /locus_tag="VF036_00320"
     CDS             1433..2494
                     /gene="thrC"
                     /locus_tag="VF036_00320"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014628363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX6263701.1"
                     /translation="MVTGAPQWRGIIEEYREWLPITADTPVITLREGGTPLVRSETLS
                     AETGCDVWLKYDGANPTGSFKDRGMTLAITKAIEEGAKAVVCASTGNTSASAAAYAAK
                     AGLTCAVLVPKGKIALGKMAQTLVHGARVLEVDGNFDAALELAKDLADRYPVTLVNSV
                     NRFRLEGQKTAAFEICDALGRAPDLHLVPVGNAGNISSHWLGYSAYLEAGTIHEPPRL
                     FGFQAQGAAPIVLGEPVKDPQTIATAIRIGNPASWDKAVSAAKESEGAILAVTDREIL
                     AAYRRVAREGLFAEPASAASVAGLLHLHAEGELTADATVVCVLTGHGLKDPEWAIAGA
                     AQPVTVPVDPAAVAAELGL"
     gene            2494..3384
                     /gene="thrB"
                     /locus_tag="VF036_00325"
     CDS             2494..3384
                     /gene="thrB"
                     /locus_tag="VF036_00325"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX6263702.1"
                     /translation="MRITVRVPATSANLGPGFDCFGLALDLCNEVIVDTEAEPGVSWE
                     GEGAGELPTDGSDMVSRAIAHTVDQQKRIHPGADAPAFRLQGLNRVPLERGLGSSSAA
                     AVAGVALARTLLGPSGFDDPYGTFAYAAELEGHPDNAAPAVYGGLTVFAPGSGYVHRL
                     DPHPSLDPVVLVPERLRLATGDARSALSSTIDRQDAVFNIGHAALAVVALTEGDLDLL
                     LVALHDRLHERVRGTMMPEVAALLDDLRHRQLAACLSGAGPSILAFERDGHVVDEPPD
                     GWRVIRPGVRATGVEVVVAG"
     gene            complement(3386..4549)
                     /locus_tag="VF036_00330"
     CDS             complement(3386..4549)
                     /locus_tag="VF036_00330"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HEX6263703.1"
                     /translation="MTVLDVALLLLLALSAWGGYRRGAILQVTGLAGLALGFLVGIRV
                     APLVADLVNSDLAKAGVELGTVLIFGVVGDAVGSILGAKLRRRAIGERTKKADALGGA
                     ALSVSALVLAIWFLGINLAAGPFPSVARALQRSSVVRAVDAALPPPPALAAQLGAVLD
                     VIGFPDIFAGLPPLPAAPVRQPTEGLAAEAARDARPSLVLISGPACDQILQGTGFVVA
                     DDLVLTNAHVVAGASPRVEWDGQTFDAVPVLFDDDLDAAILRVDGLDAPALRLEPEEV
                     ARGTGGAVLGYPHGRYTELRAAARRALDAEGRDIYSEDEIRRRVYELQVSVRPGNSGG
                     PFALPGGRVAGLVFGASLTHEDLGYALTSPRLIPLVEQVEGRAAAVGTGRCAA"
     gene            4628..5428
                     /locus_tag="VF036_00335"
     CDS             4628..5428
                     /locus_tag="VF036_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6263704.1"
                     /translation="MVILAHISDPHVGSPHFVPNMLHRVIEEINELDPAAVICSGDLT
                     TDGYRQEYQAWLSYAERIKAPLHTIPGNHDSRNVGYLHFEELVGARNWSVDVEGIRIV
                     GVDSSEPDLNEGMVGRSHYTWIREQFVQRADLKIFVLHHHLIPIPGTGRERSTVMDAG
                     DLLEVLVRSGVNVVLAGHKHVPYVWRLEDMYLASAGTCSSLRVRGHTKPCYNVLEIDR
                     GEVTIFRKFPFGERQQMARVAIATGTQMQREDEHLVQESAQAAPASAD"
     gene            5465..>5831
                     /locus_tag="VF036_00340"
     CDS             5465..>5831
                     /locus_tag="VF036_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HEX6263705.1"
                     /translation="MRTLALVDGEHYPPVTRAALAVAAERGHEVVAALLVGGTEKLEA
                     GGGLPDLGVPVRLTAADRMVALRRAIADLAPEVILDLSDEPVLGYRERMELASVALAA
                     RIPYVGADFRLDPPEEGPPL"
BASE COUNT          900 a         1987 c         2112 g          832 t
ORIGIN      
        1 gcgatcggtc tctatcggag ctgcggattc gtcgaggagg gacggctcgc gaggcactcg
       61 cgcaagtcct acggttacga ggacgagata ctcatggcgg cctggatcgg gggcgacggg
      121 tgagcgcgcg ggaagcgaac acgggggagg ggcgcaccgt ccgcatcggg ttgctcggca
      181 gcgggaccgt gggcgccgcg gtgatccggc tcctccacga gcacggcgac gacgtcgcgc
      241 ggcgggccgg cgtacacctc gaggtcgtcc gcgtggcggt gcgtgatccc ggccgtgacc
      301 gaggattacc gctcgccgcg gacaggttca ccgacgacgc cggcgcggtc gtcgacgacc
      361 cgacgatcga catcatctgt gagctgatgg gcggcgtcga gccggcccgc gggctgttgg
      421 tgcgcgcgct cgcgagcggg aagtccgtcg tcacggcgaa caaggagctg ctggcatcgc
      481 acggcgagga gctgtacacc gccgtcgagg aggccggcgg cgatctctac ttcgaggcag
      541 ccgtggccgg cggcatcccc ttgatccggc cgctccggga gtcgttggcg ggggagcgtg
      601 tcgatcggat cctcggcatc gtcaacggga cgacgaactt catcctcacc cagatgtccg
      661 aacacgggtg gacggccgac gaagcggtcg ccgaggcgca gcgactcggg ttcgcggaag
      721 cggatccgac cgccgacgtg gaggggtacg acgcggcggc gaagtgcgcg atcctcgcct
      781 cggtcgcgtt cggcgcccgg gtcgtggcca acgacgtcta ccgcgagggg atctctgcgg
      841 tcacgccgca ggacttccgg gacgcggcac gactcggcta cgtgatcaag ctgctcgcga
      901 tcgcggagct cgacgacgag gcgatcgcgg tccgggtgca tccggcgatg atccccgacg
      961 tgcacccgct cgcctccgtt cggggcgcgt cgaacgcggt cttcgtcgag ggaccgaaga
     1021 tcgggcagct gatgttctac ggcgccggcg cgggtggcga tcccacggcg accgcggtgg
     1081 tcggcgatct cgtgagcgtc gccaggaacc tgctgcaggg cgcgcgcgga ccgggtcccg
     1141 gctcgaccaa cgcgcgacgc gtccgtccga tggacgacac gcacgatcag tactacctga
     1201 agctccgcgt ggacgatcgc ccgggcgtcc tggccgagat cgccgatcgg ttcggccgca
     1261 acggcatctc gctggagcgc gtctggcagg aaggcaccgg tgaggaggcg accctgtcgt
     1321 tcatcacgca ccgggcccag gagggcgcgt tccagaagac gatgcgcgaa ctgcgtgagc
     1381 tggatgcggt gcacgccgtg gccagcgtgc tccgggtgga aggcgaggag tgatggtcac
     1441 gggggcgccg cagtggcgcg gcatcatcga ggagtaccgc gagtggctgc cgatcacggc
     1501 cgacacgccc gtgatcaccc tccgcgaggg gggaacaccc ctggtccgat ccgagacgct
     1561 gtcggccgag acgggctgcg acgtgtggct gaagtacgac ggggcgaacc cgaccggatc
     1621 gttcaaggat cgcgggatga cgctcgcgat cacgaaggcg atcgaggagg gcgcgaaggc
     1681 ggtcgtctgc gcgtcgaccg gcaacacgag cgcgagcgca gcagcgtacg cggcgaaggc
     1741 cggtctgacg tgcgcggtcc tcgtgccgaa ggggaagatc gcgctcggca agatggcgca
     1801 gaccctcgtc cacggcgccc gcgtcctcga ggtcgacggc aacttcgacg ccgcgctcga
     1861 gctcgccaag gatctggccg accggtatcc ggtgaccctc gtgaacagcg tgaaccggtt
     1921 ccggctcgaa gggcagaaga ccgcggcctt cgagatctgc gacgcgctcg ggcgagcgcc
     1981 cgacctgcac ctcgtccccg tcggcaacgc cgggaacatc tcgagccact ggctcggcta
     2041 ctccgcgtac ctcgaggccg gcacgatcca tgagccgcct cggctgttcg gcttccaggc
     2101 gcagggtgcg gcgccgatcg tgctcgggga gccggtgaag gatccgcaga cgatcgccac
     2161 cgcgatccgg atcgggaacc cggcgtcctg ggacaaggcc gtctccgcgg cgaaggagtc
     2221 cgagggcgcg atcctcgccg tgacggaccg cgagatcctc gcggcgtatc gccgcgtcgc
     2281 tcgggagggg ctcttcgctg aacccgcgtc ggccgcgagt gtcgccgggc tcctgcacct
     2341 ccacgccgag ggcgaactca cggccgacgc caccgtggtg tgcgtcctca cgggccatgg
     2401 cttgaaggac ccggagtggg cgatcgcggg ggcggcgcag ccggtgaccg tcccggtcga
     2461 ccccgcggcg gtggctgccg agctggggct gtagtgcgga tcaccgttcg ggtgccggcg
     2521 acgtcggcga acctcggtcc ggggttcgac tgcttcgggc tggcgctcga cctctgcaac
     2581 gaagtgatcg tcgacaccga ggcggagccg ggcgtgtcgt gggagggcga gggcgccggc
     2641 gagctgccga ccgacggctc ggacatggtc agccgcgcga tcgcccacac cgtcgaccag
     2701 cagaagcgga tccatcctgg tgccgacgct cccgctttcc gtttgcaggg gctgaatcgc
     2761 gtcccgttgg agaggggtct gggttcgtcg tcggctgccg ctgtggccgg tgtcgcgctc
     2821 gcgaggacac tgttgggccc ctcggggttc gacgacccct acgggacgtt cgcctacgcc
     2881 gcggagctcg agggtcatcc ggacaacgcg gctcccgcgg tctacggagg actcaccgtg
     2941 ttcgcgcccg gttcgggcta cgtgcatcgc ctcgatccgc atccctccct cgatccggtc
     3001 gtcctggtgc cggaacggct ccgactcgcg accggggacg ctcggagcgc gctctcgtcg
     3061 acgatcgatc gacaggatgc ggtgttcaac atcggccacg cggcgctcgc cgtcgtggcg
     3121 ctcacggaag gagatctgga tctcctgctc gtggcgctcc acgatcgtct tcacgagcgc
     3181 gtccgaggaa cgatgatgcc cgaggtcgcg gcgctcctcg acgacctgcg tcatcgtcag
     3241 ctcgccgcct gcctctccgg ggccgggccg tcgatcctcg cgttcgagcg agacggccat
     3301 gtcgtggacg agccgccgga cggctggcgt gtgatccggc cgggcgtgcg tgccacgggc
     3361 gtggaggtcg tcgtcgcggg ctgagtcagg cggcacagcg gcccgtgccg acggcggccg
     3421 cgcgaccctc gacctgttcg acgaggggga tcaaccgtgg ggacgtgagc gcgtagccga
     3481 gatcctcgtg cgtgagcgac gccccgaaga cgagacccgc caccctgccg ccgggcagcg
     3541 cgaacggccc tccgctgttc ccgggtcgca cgctgacctg gagctcgtag acccgccgac
     3601 ggatctcgtc ctccgagtag atgtcgcggc cctcggcatc caacgcccgc ctggcggcgg
     3661 cgcgaagctc cgtgtacctg ccatgcggat agccgagcac ggcgccgccc gtccctcgcg
     3721 caacctcttc cggctcgagc ctgagcgccg gcgcgtccag accgtcgact cgcaggatcg
     3781 cggcgtcgag atcgtcgtcg aagagcaccg ggaccgcgtc gaacgtctgc ccatcccact
     3841 cgacccgcgg gctggcgccg gcgaccacgt gcgcgttggt cagcacgaga tcgtcggcga
     3901 cgacgaaccc ggttccctgc aggatctgat cgcacgcagg gccggagatc agcacgagcg
     3961 aggggcgggc gtcccgagcc gcctcggccg cgagcccctc cgtcggctgg cgcaccggtg
     4021 ccgcgggaag cggcggcagt ccggcgaaga tatccgggaa cccgatcacg tcgaggacgg
     4081 cgccgagctg cgccgcgagg gcgggagggg gcgggagcgc cgcatcgacc gctcgaacga
     4141 cggaggagcg ttgcagtgca cgggcgaccg aggggaacgg acccgcggcg aggttgatcc
     4201 ccaagaacca gatcgcaagg acgagggcgc tcacggacag ggcggctccg ccgagcgcgt
     4261 cggccttctt ggtgcgctcc ccgatcgcgc gtcgacgcag cttcgcgccg aggatcgacc
     4321 ccaccgcgtc ccccacgacc ccgaagatga gcaccgtgcc gagctcgaca cctgccttgg
     4381 ccaggtcgga attgacgaga tcggcgacga gcggcgccac gcggatgccg acgaggaacc
     4441 cgagggcgag accggcgagc cctgtcacct ggaggatggc gccgcgtcga tacccgcccc
     4501 aagcggacag tgcgagcaac agcaacagag ccacatccag gacggtcacg gagcgacatc
     4561 gtcccatgcg tcgccgcgcg gatcgcgagc gacgtcatcc gccgtcctct ccccctacac
     4621 tgccgacgtg gtcatcctcg cccacatctc cgacccgcat gtgggttcgc cccacttcgt
     4681 gccgaacatg ttgcatcgcg tgatcgagga gatcaacgag ttggatcccg cagccgtgat
     4741 ctgcagcggc gacctgacga ccgacggcta tcggcaggag taccaggcgt ggctgtcgta
     4801 cgcggaacgg atcaaggcgc ctctccacac gatccccggg aaccacgact cccgcaacgt
     4861 cgggtacctg catttcgagg agctggtcgg tgcccgcaac tggtccgtcg acgtggaggg
     4921 catccggatc gtcggggtgg actccagcga acccgacctg aacgagggga tggtggggcg
     4981 cagccactac acctggatca gggagcagtt cgtacagcgt gccgatctca agatcttcgt
     5041 cctgcaccac catctgatcc cgatccccgg tacgggccgg gaacggagca ccgtgatgga
     5101 cgccggggat ctgctcgagg tcctcgtgcg atcgggggtg aacgtggtcc ttgcgggtca
     5161 caaacatgtt ccgtacgtgt ggcggctcga ggacatgtat ctcgccagcg ccggcacgtg
     5221 ctcctccctc cgggtgcgag gacacacgaa gccctgctac aacgtgttgg agatcgaccg
     5281 cggcgaggtc acgatcttcc ggaagttccc gttcggtgag cggcagcaga tggcgcgggt
     5341 cgcgatcgcc acgggaacgc agatgcagcg cgaggacgaa cacctcgtac aggagtccgc
     5401 gcaggccgct cctgcttcgg ccgactgaac cgcaggaccc gacccgtcca ccccaggaga
     5461 ccacatgcga acgctcgccc tcgtcgatgg tgagcactac ccgccggtga cgcgcgcggc
     5521 gctcgccgtc gccgctgagc gcgggcacga ggtcgtggcc gcgctcctcg tgggcggcac
     5581 cgagaagctc gaggcggggg gagggctccc ggatctcggc gtcccggttc ggttgacggc
     5641 cgcggatcgg atggtcgcgc tacgccgggc gatcgcggac ctggccccgg aggtgatcct
     5701 ggatctgtcc gacgaacccg tgctcggcta ccgagagcgg atggagctcg cctccgtcgc
     5761 gctcgccgcg cggatcccct acgtcggggc ggacttccgc ctcgaccctc cggaggaagg
     5821 cccaccgctg c
//
LOCUS       DASUVE010000015         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_946186, whole genome shotgun sequence.
ACCESSION   DASUVE010000015 DASUVE010000000
VERSION     DASUVE010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946186"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VF036_00345"
     CDS             <1..420
                     /locus_tag="VF036_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263706.1"
                     /translation="EVVMIWTATGGYLDPFPVEDARRFVEEFTASLDSRTDVLGTIKE
                     SGDLSDETEATLKESVEAFAQTFRTSEAIAGSEAGRGAGTAPDEVKPDIGWDRMSSVE
                     DEDEEETGGPSLAEEDDVQGQVPLPDDEDDTAGESPG"
     gene            421..1314
                     /locus_tag="VF036_00350"
     CDS             421..1314
                     /locus_tag="VF036_00350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX6263707.1"
                     /translation="MGAQLRIVRRRIRTVQSTKKITRAMELIASSRIVKAQQRVEASR
                     PYAIQLTKAMEDLARQTGAVQHPLLEDREQPTKAGVLVLTSDRGLAGAYNANVLKVAE
                     GVIREVRSRGLEPVLYAIGKKGIGYFRFRGIPMRERWQGISEVPPYEEAERIGRTLIK
                     DFADEEIDELHAAYTDFRSAFTLRATAQRFLPIAPEEVTGKGLGVVHAEYLFEPEPDE
                     ILDHLLPQYVVTKVYAALLESAASENAARRRAMKAATDNAEDLIKILTRQANRARQDE
                     ITTEIMEIVGGAESMAAGSEG"
     gene            1311..>2430
                     /gene="atpD"
                     /locus_tag="VF036_00355"
     CDS             1311..>2430
                     /gene="atpD"
                     /locus_tag="VF036_00355"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041939784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX6263708.1"
                     /translation="MTMPGTSEKSHPEGRVVRVIGPVVDVEFPPAELPEVHTALTVDL
                     TLESETTTITCEVAQHIGDSIVRTIALKPTDGLVRGTPVVNTGAPISVPVGEGVLGHV
                     YNVIGEPLDTDASEIHASDRWPIHREPPQFSELEPKKQMLETGIKVIDLLEPYVQGGK
                     IGMFGGAGVGKTVIIQEMIRRQAKEHGGVSVFAGVGERTREGNDLFLEMTESGVINNT
                     ALVFGQMDEPPGVRLRVALSALTMAEYVRDVERKDVLLFIDNIFRFTQAGSEVSTLLG
                     RMPSAVGYQPTLADEMGELQERITSAGGRSITSLQAIYVPADDITDPAPHTAFAHLDA
                     TTVLSRDIAALGIYPAVDPLDSTSRILDARYLGEEHYAV"
BASE COUNT          435 a          796 c          866 g          333 t
ORIGIN      
        1 gaggtcgtga tgatctggac cgccaccggc ggatacctcg atccgttccc ggtggaggac
       61 gcgaggcggt tcgtcgagga gttcacggcc tccctcgact cccgaacgga cgtcctcggc
      121 acgatcaagg agtcgggtga cctgtccgac gagaccgagg ccacgctgaa ggaatccgtc
      181 gaggcgttcg cccagacgtt ccgcacgagc gaggcgatcg caggcagcga ggccggtcgt
      241 ggcgccggga cggccccgga cgaggtgaag ccggacatcg gttgggatcg gatgtcctcc
      301 gtggaagacg aggacgagga agaaaccggc ggtccctcgc tcgccgagga agacgacgtg
      361 cagggccagg tgccgttgcc ggacgatgag gacgatacgg ctggggagag tcccgggtag
      421 atgggcgctc agctccggat cgttcgccgg cggatccgaa cggtgcagtc gacgaagaag
      481 atcacgcgcg cgatggagct gatcgcgtcc tcgcgcatcg tgaaggcgca gcagcgtgtc
      541 gaggcgtcgc gcccgtacgc gatccagctc accaaggcga tggaggacct cgcgcgccag
      601 acgggcgccg tccagcaccc gctcctcgag gatcgggagc agcccaccaa ggccggcgtg
      661 ctcgtgctca cctcagaccg gggcctcgcc ggcgcgtaca acgcgaacgt gctgaaggtg
      721 gcggaagggg tgatccgcga ggtccgcagc cgagggctcg agccggtcct gtacgcgatc
      781 ggcaagaagg gcatcggcta tttccggttc cgcgggatcc ccatgcgcga gcgttggcaa
      841 gggatcagcg aggttccgcc gtacgaggag gcggagcgga tcggtcgcac cctgatcaag
      901 gacttcgccg acgaggagat cgacgagctg cacgccgcct acaccgactt ccgctccgct
      961 ttcacgctcc gcgcgaccgc gcagcggttc ctgccgatcg caccggagga ggtcacgggg
     1021 aagggcctcg gggtcgtcca cgccgagtac ctcttcgagc ccgagccgga cgagatcctc
     1081 gaccacctgc tgccgcagta cgtggtgacg aaggtgtacg cggccctgct ggaatcggcc
     1141 gcctcggaga acgcggcccg gcgccgtgcg atgaaggccg ccaccgacaa cgcggaagac
     1201 ctcatcaaga tcctcacgcg ccaagccaac cgggcgcgcc aggacgagat cacgacagag
     1261 atcatggaga tcgtgggcgg cgccgaatcg atggcggccg gctcggaggg atgacgatgc
     1321 caggaacgag tgagaagtcg caccccgagg ggcgcgtggt ccgggtgatc ggaccggtgg
     1381 tagacgtgga gttcccgccg gcggagctgc ccgaggtcca cacggcgctc accgtggacc
     1441 tcaccctcga gtccgagacg accacgatca cgtgcgaggt cgcgcagcat atcggtgact
     1501 cgatcgtccg caccatcgcc ctcaagccca cggacggcct cgtccgcggc acgccggtcg
     1561 tgaacacggg ggcgccgatc agcgtgccgg tgggtgaggg cgtcctgggg cacgtctaca
     1621 acgtgatcgg ggagcccctg gacacggacg cctccgagat tcacgcgagc gaccgctggc
     1681 cgatccaccg ggagccgccg caattctcgg agctcgagcc caagaagcag atgctcgaga
     1741 ccgggatcaa ggtgatcgac ctcttggagc cgtacgtcca gggcggcaag atcgggatgt
     1801 tcgggggcgc cggcgtcggc aagaccgtga tcatccagga gatgatccgt cggcaggcca
     1861 aggagcacgg aggcgtctcc gtgttcgccg gggtggggga acggacccgc gagggcaacg
     1921 acctcttcct cgagatgacg gagtcgggcg tcatcaacaa cacggcgctc gtgttcggac
     1981 agatggacga gccgccgggc gtgcggctcc gggtcgcgct ctcggcgctc acgatggccg
     2041 agtacgtccg cgacgtcgag cggaaggacg tcctgctgtt catcgacaac atcttccggt
     2101 tcacgcaggc gggctccgag gtctccacac tcctgggacg catgccctcc gcggtgggct
     2161 accagccgac gctcgccgac gagatgggcg agctccagga gcggatcacc tcggccggcg
     2221 ggcggtcgat cacgtccctc caggcgatct acgtgccggc ggacgacatc accgacccgg
     2281 ccccgcacac ggcgttcgcg cacctcgatg cgaccaccgt gctcagccga gacatcgcgg
     2341 cgctcgggat ctacccggcg gtcgacccgc tcgactcgac gagccggatc ctcgacgctc
     2401 gatatctggg ggaggagcac tacgcggtcg
//
LOCUS       DASUVE010000016         2194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_383143, whole genome shotgun sequence.
ACCESSION   DASUVE010000016 DASUVE010000000
VERSION     DASUVE010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383143"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VF036_00360"
     CDS             complement(<1..611)
                     /locus_tag="VF036_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263709.1"
                     /translation="MLAGRGVAVFVAGIAMWLAARFLGSPGLEVVAIGIAALPLIASF
                     AAKRGAQRISVRRRLSDVRISPGSRVTVTLDVENRSPVGTSFLLLEDRLPTPLGRAAR
                     LVIGGIRAGKREEATYTLVPQSRGRYLLGPLVIDVSDPFALSRVRLEFDEREELLVAP
                     EIEDLGTAPDPASGPSFGASRARQLFRTGQEYYTMRSYQEGDDL"
     gene            complement(628..1602)
                     /locus_tag="VF036_00365"
     CDS             complement(628..1602)
                     /locus_tag="VF036_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6263710.1"
                     /translation="MEVREKIPDPRAFAARFREIEENVERVVRGKHKEIRLALVALVA
                     EGHLLIEDVPGVGKTMLAKSIARSIDCSFRRIQFTPDLLPTDVTGVNVFSQETGDFEF
                     KPGAIFANIVLGDEINRASPKTQSALLECMEERQVTVDTETHTLGTPFIVIATQNPIE
                     HEGTYPLPEAQLDRFMMRLAIGYPDAEVAAEILASHAAGAPLDDIRPVSDASGVREMI
                     EEARRIHAAPPIRRYIVDLVEATRRHSDVYLGASPRASIMILRAARALAAAEERDYVI
                     PDDVKTLAPVALPHRIIVTADAIMSGRSAEVLVRQILDEIDVPIAENP"
     gene            1931..>2194
                     /locus_tag="VF036_00370"
     CDS             1931..>2194
                     /locus_tag="VF036_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX6263711.1"
                     /translation="MGRALVLNASESPLAVVAARRAVVMVLKEKADVILSNGMVFHSA
                     RLTIEAPSVVRLRRYVHVPYRPHAPLTRRAVFARDLGTCQYCGA"
BASE COUNT          345 a          729 c          769 g          351 t
ORIGIN      
        1 aggtcgtcgc cctcctgata ggaccgcatc gtgtagtact cctggccggt ccggaacagc
       61 tgtcgggcac gcgacgcgcc gaacgacggc cccgaggcgg gatcgggggc ggtgccgagg
      121 tcctcgatct cgggggcgac cagcagttcc tcccgttcgt cgaactccag gcgcacccgg
      181 ctcagcgcga acggatccga cacgtcgatc acgaggggtc cgaggagata gcgccctcgc
      241 gactgaggga ccagcgtgta cgtcgcctct tcccgtttcc cggcacggat cccaccgatc
      301 acgagccgcg ccgctcgtcc gaggggcgtc gggagccggt cctccagcag gaggaacgag
      361 gtgccgaccg gggagcgatt ctcgacgtcg agcgtcacgg tgacccttga gcctggactg
      421 atccggacgt cggacagccg ccgccggacg gagatccgtt gggcgccacg cttcgcagcg
      481 aacgaggcga tcaatgggag ggcggcgatg ccgatcgcca cgacctccag gccgggggag
      541 ccaaggaacc gggccgcgag ccacatcgcg atgcccgcca cgaagacggc cacgccgcgt
      601 cccgcgagca tcagtgatcc gggagcatca ggggttctcc gcgatgggga cgtcgatctc
      661 gtcgaggatc tgccgaacga gcacctccgc ggagcgcccg ctcatgatcg cgtccgccgt
      721 gacgatgatg cggtggggca gggcgaccgg tgcgagggtc ttcacgtcat cggggatgac
      781 gtagtcccgc tcctcggctg ccgcgagcgc gcgcgcggcg cgcaggatca tgatcgaggc
      841 ccgcggactg gcgccgagat agacgtcgga gtgccgccgt gtcgcttcca ccagatcgac
      901 gatgtacctg cggatcggtg gagctgcgtg gatcctccgc gcctcctcga tcatctcgcg
      961 gacgccgctc gcgtcgctca cgggccggat gtcgtcgagc ggggccccgg ccgcatggct
     1021 cgccaggatc tctgcggcga cctccgcatc gggatacccg atcgcgaggc gcatcatgaa
     1081 tcggtcgagc tgtgcctccg ggagcggata ggttccctcg tgctcgatcg ggttctgggt
     1141 ggcgatcacg atgaacggtg ttccgagggt gtgcgtctcg gtatcgaccg tgacctgccg
     1201 ctcctccatg cactcgagga gggcggactg ggtcttggga gacgcccggt tgatctcgtc
     1261 cccgagaacg atgttggcga agatcgcacc gggcttgaac tcgaagtccc cggtctcctg
     1321 gctgaagacg ttgacgccgg tgacatcggt gggcaacaga tccggtgtga actggatccg
     1381 ccggaacgag cagtcgatcg agcgggcgat cgacttggcg agcatggtct tgccgacgcc
     1441 gggtacatcc tcgatcagca ggtggccctc ggcgacgagg gcgacgaggg cgagcctgat
     1501 ctccttgtgc ttgccgcgga cgacccgttc gacgttctcc tcgatctcac ggaaccgagc
     1561 agcgaacgcc cggggatccg ggatcttctc tcgtacctcc atcgcatccg acccctgacc
     1621 tcggtagcag gtgcacaatc ttacgtgcgg gacggaaccg gggttgcgtc gaggtagcag
     1681 acgcccgccg gtgagggcaa gttcccgaca cgtcgtcgga ggccggccgc gacgacagcg
     1741 aagcgggttc ggaggccgcg gatcccgggc tcgagggcgg accgacccac gaacggacgc
     1801 cccgcgcggc ccctagtcgc aagatcaggc atcgcgcgcg ggccggcccg ccttcgctcg
     1861 acagattaca cgcttgtgat tcgcgcgcct gtatccctcg tggttcctgc gaggtcgcta
     1921 cactgcgcgc atgggacgcg ccctcgtgct caacgcgtcg gagtcgccgc tcgcggtggt
     1981 ggcagcgaga cgggcggtcg tcatggtgct caaggagaag gccgacgtca tcctcagcaa
     2041 cgggatggtg ttccactcgg cccggttgac gatcgaggcg ccctccgtgg tccgactccg
     2101 tcggtacgtg cacgtcccgt accggccgca tgcgccgctg acgcgacgag cggtgttcgc
     2161 ccgtgacctc ggcacgtgtc aatactgcgg cgcc
//
LOCUS       DASUVE010000017         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807 k141_7844,
            whole genome shotgun sequence.
ACCESSION   DASUVE010000017 DASUVE010000000
VERSION     DASUVE010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7844"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..1585
                     /locus_tag="VF036_00375"
     CDS             290..1585
                     /locus_tag="VF036_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015938750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6263712.1"
                     /translation="MPHREDRPTTRTRRRRRLPLALATAGLLGATVALSGCAGVGGGG
                     PAEITFHMSKPEAIPFFRDLVQRYNGEQSDVRVTLDTASNLSAGFLRGNPPDVGLLNY
                     NYEMARFMERGALSDLSDMPEAQRIRPDVQELVDQYATYPGRTSVLPYSVAAASVIYN
                     VEIFEENGIEVPTTWDELIDVCDTLQAAGVTPIYSTFKDTWTLGQGLFDYSVGGLIDV
                     PQFLDEMEELGTEVGPESEVSFQKTILEPSEKMKQLADYSNADAESRGYGDGNVAFAN
                     GEAAMYMQGPWAFGEIAKTNPDLELATFPLPSSDDPDDLKVRVNLDLALWIPEASTEQ
                     EAAREFVSYLMQQDVMDEYNAAFLGFGTTTDAAPVTDERILPMQEYYDDGAFYQGLSR
                     SIPLTIPIDNYIQTMATGGDIPATLAKIDADWARLALRG"
     gene            1612..2580
                     /locus_tag="VF036_00380"
     CDS             1612..2580
                     /locus_tag="VF036_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6263713.1"
                     /translation="MSTPITPAAAAAGSLVDEAEHGRVDHLAGASHDKRGRPRVDPIY
                     YWFLVPTLVLFTLAITVPAVIGIFYSFTNFIGFGDWEFIGLTNYVAVFSDPAILASYG
                     FTFGFAIVTVLLVNVIAFLLAVGLTSRIRLKTPLRAVFVIPMVISGIVIAYVFNFLFS
                     NSLPAFGTAAGIPWLSESILANPDLAWLSIVIVTAWQSIPATLLIYIAGLLSIPGDVY
                     EAADIDGATPARRLWSVTLPLVAGYVVINIIIGFKNYLNVYEVIVGLTNGGPGTATRS
                     IAMTIFTGFTGGDYAYQMANATIFFIIAVAISVLQLRLTRGKAATS"
     gene            2577..>2757
                     /locus_tag="VF036_00385"
     CDS             2577..>2757
                     /locus_tag="VF036_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6263714.1"
                     /translation="MTQAPAKRIAEPATRTLGRSEATATTTTRRRRGRVGQERTNWAT
                     MVILLLCALTVLLPLY"
BASE COUNT          461 a         1015 c          827 g          454 t
ORIGIN      
        1 ttcgtcgcag acgccgatga cggtggagcg cgtgagcccg gtggctgcca tcgcatcggt
       61 cgcggtgaac gcgccggccc gccaggcgtg ggcgagcagc cggcgggcgt tcacccgtcg
      121 gagcacctgc ggcgacgtcg ctgcttcgat cctgtccacc acttgacctt tccgtgaccc
      181 tcagtcatta tttacgacaa caagtaaatc tacactctat atttacttcc gtcagggtcg
      241 acgagccggg ttcggcgcgg ccgatccgga accacgagga gacgacgagg tgcctcaccg
      301 cgaagaccgc cccaccaccc gcacccggag gcgacggcga ctgccgctcg cgctcgcgac
      361 cgcgggtctc ctcggcgcca cggtcgccct gtccggctgc gccggcgtcg gcggcggcgg
      421 tcccgccgag atcacgttcc acatgtccaa gcccgaggcg atcccgttct tccgcgacct
      481 cgtgcagcgg tacaacggcg agcagtccga cgtccgcgtg accctcgaca ccgcgtcgaa
      541 cctgtccgcg ggcttcctcc gcggcaatcc gcccgacgtc ggactcctca actacaacta
      601 cgagatggcg cgcttcatgg agcgcggggc gctgtccgac ctctccgaca tgcccgaggc
      661 ccagcgcatc cgccccgacg tgcaggagct cgtcgaccag tacgccacgt accccggacg
      721 aaccagcgtg ctgccctact ccgtcgccgc cgcgtcggtg atctacaacg tcgagatctt
      781 cgaggagaac ggcatcgagg tgcccaccac gtgggacgag ctgatcgacg tctgcgacac
      841 gctccaggcc gccggtgtca ccccgatcta cagcacgttc aaggacacct ggacgctcgg
      901 tcagggtctc ttcgactact ccgtcggcgg cctgatcgat gtcccgcagt tcctcgacga
      961 gatggaggag ctcggcacgg aggtcggtcc cgagtccgag gtgtccttcc agaagacgat
     1021 cctcgaacca tccgagaaga tgaagcagct cgccgactac tcgaacgccg acgccgagag
     1081 ccgcggctac ggagacggca acgtcgcctt cgcgaacggc gaggccgcca tgtacatgca
     1141 gggcccgtgg gcgttcggcg agatcgcgaa gacgaacccc gacctcgagc tcgccacctt
     1201 cccgcttccc tcgtcggacg accccgacga cctcaaggtg cgggtcaacc tcgatctcgc
     1261 cctgtggatc cccgaggcga gcaccgagca ggaggccgcg agggaattcg tgtcgtacct
     1321 catgcagcag gacgtgatgg acgagtacaa cgccgcgttc ctcggcttcg gaaccaccac
     1381 ggatgccgcg ccggtgaccg acgaacgcat cctcccgatg caggagtact acgacgacgg
     1441 cgccttctac cagggcctct cccggtcgat cccgttgacc atcccgatcg acaactacat
     1501 ccagacgatg gcgaccggcg gggacatccc cgcgacactc gccaagatcg acgccgactg
     1561 ggcgcggctc gccctgcgcg gctgacccgg cgaatcgaga ggacgaacgc catgagcaca
     1621 cccatcacgc ccgccgcggc cgccgccggc tcgctcgtcg acgaagccga gcacggccgc
     1681 gtcgatcacc tcgcgggcgc ctcccacgac aagcgcgggc ggcccagggt cgatccgatc
     1741 tactactggt tcctcgttcc cacgctggta ctgttcaccc tcgcgatcac ggtccccgcg
     1801 gtgatcggca tcttctactc gttcacgaac ttcatcgggt tcggtgactg ggagttcatc
     1861 gggctcacca actacgtcgc ggtgttctcc gacccggcga tcctcgccag ctacggcttc
     1921 acgttcggct tcgcgatcgt gacggtgctg ctcgtcaacg tgatcgcctt cctcctcgcg
     1981 gtggggctca cgtcgcgcat caggttgaag acgccgcttc gcgctgtgtt cgtgatcccg
     2041 atggtgatct cgggcatcgt gatcgcctac gtcttcaact tcctcttctc caactcgctg
     2101 ccggcattcg gcacggccgc cggcatcccc tggctgtcgg agtcgatcct ggcgaacccc
     2161 gacctcgcct ggctctcgat cgtgatcgtg acagcctggc agtcgatccc ggcgacgctg
     2221 ctgatctaca tcgccggact gctctcgatc ccgggcgatg tgtacgaggc cgccgacatc
     2281 gacggggcga ccccggcccg ccggctctgg tcggtgacgc tcccgctcgt cgcggggtac
     2341 gtcgtgatca atatcatcat cggcttcaag aactacctga acgtgtacga ggtcatcgtc
     2401 ggactcacca acggcggccc cggcacggcg acgcgcagca tcgcgatgac gatcttcacc
     2461 ggattcaccg gcggtgacta cgcctaccag atggccaacg cgacgatctt cttcatcatc
     2521 gccgtggcca tctccgtact ccagctccga ctcacccgcg gaaaggcggc gacctcatga
     2581 cccaggcacc tgcgaagcgc atcgccgaac ccgcgacccg gacgctcggg cgcagcgagg
     2641 cgaccgccac gacgaccacc cggcgccgcc gcggccgggt cggccaggag cggacgaact
     2701 gggcgaccat ggtcatcctg ctcctctgcg ccctgaccgt gctcctcccg ctctacg
//
LOCUS       DASUVE010000018         4814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1139415, whole genome shotgun sequence.
ACCESSION   DASUVE010000018 DASUVE010000000
VERSION     DASUVE010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139415"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VF036_00390"
     CDS             <1..710
                     /locus_tag="VF036_00390"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX6263715.1"
                     /translation="HVLEAEGVAPLGVAGHSLGQFAALVAADVVSLADALDLVVIRGA
                     AMQRAGEERPGAMTALLGVGSGDASWLCEEVRGDDELVVANENSPAQVVISGSVAAIE
                     RAESLAKERRIRAVRLPVAGAFHSPLMEPARAAIDERIDATGFSAPRCPVAENVAGAL
                     VSDPDELRSLLKRHVVSPVRWESSILALGDAGAGSFLEAGPGDVLTKLMKRIVPGAAA
                     RAIGSPEEARVAASGTP"
     gene            746..1744
                     /locus_tag="VF036_00395"
     CDS             746..1744
                     /locus_tag="VF036_00395"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX6263716.1"
                     /translation="MTRSATIIGVGSTLPPRLVPNTWFEDKIDTNDEWIRERTGIEAR
                     HFVEEGVDTSDLVVDAARAAMTTAAITPEQLDMIVVASVTGDTPFPATAVWAQEKLGV
                     GVPAFDVNAACAGFSYGLASATAFVQAGMADTVLLAGAEIFSRILDFTDRTTSILFGD
                     GAGAVIVQASDRPGIEGTVLGADGTAADILQMPAGGTRVPASHATVDARDHSIRMPKG
                     REVFKRAVTEMAAACRELLEKSEHTPADVDVLIPHQANARIMVAVAERLGIPAERSIL
                     DVAEVGNTSAASIPIALARAWNAGRIKEGDLVLLTSFGAGLTWGATLVRWTMQEPE"
     gene            1741..2478
                     /gene="fabG"
                     /locus_tag="VF036_00400"
     CDS             1741..2478
                     /gene="fabG"
                     /locus_tag="VF036_00400"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX6263717.1"
                     /translation="MTPKVAVISGASRGIGRACSQALAAAGWTVAVGYRTREADAKET
                     LEALESAGTPGMTVYLDVTDESSVQEAFRRVNDEVGPVIGLVNNSGYSRDGLLLKYSM
                     EEYERTMTTNLRGSFLCSRAALRSMLREKWGRIVNMSSAVAIHGNAGQTVYAATKSGL
                     LGLTKSLAREVGGKGITVNALCPGLLQTEMTAHLTDDARAFYLDQTPVRRAATLEEVS
                     AVVRFLMSEEASYVNGTVIPVDGGLTA"
     gene            2568..2816
                     /gene="acpP"
                     /locus_tag="VF036_00405"
     CDS             2568..2816
                     /gene="acpP"
                     /locus_tag="VF036_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011057709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX6263718.1"
                     /translation="MDRAEIETRVKKVLAEQLAVDEQQVIPDARFAEDLNADSLDLVE
                     AVLALEEEWSIEIPEDEMESVKTVGQAVDLVASKLGMS"
     gene            2824..4095
                     /gene="fabF"
                     /locus_tag="VF036_00410"
     CDS             2824..4095
                     /gene="fabF"
                     /locus_tag="VF036_00410"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX6263719.1"
                     /translation="MSLTTEVPAPRPHAGRKVVVTGIGPVTPVGTGVEDFWTGLTTGR
                     NGVREIARFATDDLPVKVAGDVHDFDPSLWLDPKEIRRTDRFSHYAIAAGKLAWEDAG
                     TPDVPSERGGIVFGTGIGGIETLLVQHSVLLEKGPGRVSPFMVPALMANAAAGHLAMK
                     FGLTGPNYCTVSACASSNHAIGEGMRLIRDGYLDVCITGGSEAATLPLTVAAFAQMTA
                     LTKNPDPESASRPFDANRNGFVLSEGAASLILESEEHARARGARIYCEVAGYGMSDDA
                     HHITAPDPKGSGAALAMRWALADAGEEPASVRYVNAHGTSTPLNDASETVAIKAVLGD
                     EIAHEVAISSTKSMTGHMLGAAGAVEGAVCALAIQRGVVPPTIHYETPDPDCDLDYVP
                     NEARELDVTLALSNSFGFGGQNACVAFRRVA"
     gene            complement(4109..>4814)
                     /locus_tag="VF036_00415"
     CDS             complement(4109..>4814)
                     /locus_tag="VF036_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263720.1"
                     /translation="EGMACVILQNGAGDKRGVMGATLAAVRAGLAGDELPAPWAPPAA
                     TAIPARDDFVGTYAGEDGRTLEVRKDEGGLSISIGSVSARLERDPLVADPGQSFLAAH
                     PAFERFPFEFRRDEAGKVVEAFHGGTWFRGEAYEGPDPEEPPAGWDRHPGIYRNDDPW
                     SAVLRVVLRKGRLAILWPTDASDEEGGELIPLDDGSFAVGDPAVPLRVRFEGDVRGMT
                     AVVVCNGGRWFRSFEE"
BASE COUNT          756 a         1681 c         1700 g          677 t
ORIGIN      
        1 tccacgtcct cgaagccgaa ggcgtcgccc cgctcggcgt ggcaggtcac tccctcggtc
       61 agttcgcggc gctcgtagcg gccgacgtcg tctccctggc cgacgccctc gatctggtgg
      121 tgatccgggg cgccgcgatg cagcgggccg gggaggagcg tcccggagcg atgacggcgc
      181 tgctcggcgt cggctccggg gacgcttcgt ggctctgcga ggaggtccgt ggagacgacg
      241 agctcgtggt ggcgaacgag aactccccgg cgcaggtggt gatctccggc tcggtcgcag
      301 cgatcgagcg agccgagtcg ctcgccaagg agcggaggat ccgcgcggtt cgactcccgg
      361 tcgccggtgc gttccactcg ccgctgatgg agccggcgcg cgcggcgatc gacgagcgca
      421 tcgacgcgac cgggttctcc gcgccccggt gcccggtcgc ggagaacgtg gcaggcgccc
      481 tcgtctccga ccctgacgag ctccgctccc tcctgaagcg acacgtcgtg tctccggtgc
      541 gatgggagtc ctcgatcctc gccctcggtg atgcaggggc gggctcgttc ctcgaagcgg
      601 gtcccggcga cgtgctcacc aagctgatga agcggatcgt gcccggggcg gcagcccggg
      661 cgatcggctc acccgaggag gcgcgggtcg ccgcgtccgg cacgccctga ccgactgtgt
      721 gccgccgacc gactacactc ccgccgtgac caggagcgcc accatcatcg gcgtcgggtc
      781 cacgctccct ccgcgcctcg ttcccaacac gtggttcgag gacaagatcg acaccaacga
      841 cgagtggatc cgggagcgga ccgggatcga ggcccgccac ttcgtcgagg agggcgtcga
      901 cacgtcggac cttgtggtcg acgcggcgcg ggcagcgatg accacggcgg cgatcacgcc
      961 ggaacagctc gacatgatcg tcgtcgcgag cgtcacgggc gacacgccgt tccccgcgac
     1021 cgccgtctgg gcccaggaga agctgggcgt cggcgtgccg gcgttcgacg tgaacgcggc
     1081 atgcgcgggc ttcagctacg gcctcgcgag cgccaccgcg ttcgtgcagg cggggatggc
     1141 cgacacggtc ctcctggcgg gggcggagat cttcagccgg atcctcgact tcacggaccg
     1201 cacgacctcg atcctcttcg gcgatggcgc gggcgccgtg atcgtccagg cctccgaccg
     1261 gcccgggatc gaggggaccg tcctcggcgc tgacgggacg gcggccgaca tcctccagat
     1321 gccggcggga ggcacgcgcg tgcccgcctc ccatgccacc gtcgacgccc gcgatcactc
     1381 gatccggatg ccgaaggggc gagaggtgtt caagcgcgcg gtgaccgaga tggccgccgc
     1441 ctgccgggag ctcctcgaga agtccgagca cacgcccgcc gacgtcgacg tcctgatccc
     1501 tcaccaggcg aacgcccgca tcatggtcgc cgtcgcggaa cgactcggaa tccccgcgga
     1561 acggtcgatc ctggacgtgg ccgaggtcgg caacaccagc gccgcctcga tcccgatcgc
     1621 gctcgcgcgg gcatggaacg cgggccggat caaggagggc gacctcgtcc ttctcacgtc
     1681 gttcggcgcc ggtctcacct ggggcgccac gctggtccgg tggacgatgc aggagcccga
     1741 gtgaccccca aggtcgccgt gatctcgggc gcctcccgcg ggatcggtcg cgcgtgctcc
     1801 caggcgctcg ccgccgccgg atggacggtc gccgtcggct atcggacccg cgaggccgac
     1861 gccaaggaga cgctggaagc cctggagtcg gccggaacgc ccggcatgac cgtgtacctc
     1921 gacgtgaccg acgagtcgag cgtccaggag gcgttccgac gggtcaacga cgaggtcggt
     1981 ccggtcatcg gcctcgtgaa caactcggga tactcgcggg acgggctcct gctgaagtac
     2041 tcgatggagg agtacgaacg gacgatgacc acgaacctcc gcggatcgtt cctgtgctcc
     2101 agggccgcac tccggagcat gctgcgcgag aagtggggcc ggatcgtgaa catgtcgagc
     2161 gcggtggcga tccacggcaa cgcggggcag accgtgtacg cggcgacgaa gtcgggcctc
     2221 ctcgggctca cgaagtccct cgcccgcgag gtgggcggca agggcatcac ggtgaacgcg
     2281 ctctgtcccg ggttgctgca gaccgagatg accgcgcacc tcaccgacga cgctcgcgcg
     2341 ttctatctcg accagacccc ggtgcgtcgg gcggccacgc tcgaggaggt ctcagccgtc
     2401 gtgcgattcc tgatgtcgga ggaagcgtcc tacgtcaatg gcacggtgat cccggtcgat
     2461 ggggggctca ccgcatagtc ggtgatccgg tccgcggata ggctcgcccg gaacgagtca
     2521 aacggagaga gaagacgaga acccagagac agaagaggag aggaagcatg gatcgtgcgg
     2581 agatcgagac cagggtgaag aaggtgctgg ccgagcagct ggccgtcgac gaacagcagg
     2641 tcatccccga cgcccggttc gccgaggact tgaacgccga ctccctcgac ctggtcgagg
     2701 cggtgctcgc cctggaagag gaatggagca tcgagatccc cgaggacgag atggagtccg
     2761 tgaagacggt cggccaggcg gtcgacctcg tcgcgagcaa gttggggatg tcgtaagccg
     2821 ttcatgtcgc tcacgaccga agtacccgct ccccgaccgc atgccggtcg gaaggtcgtc
     2881 gttaccggca tcggcccggt tacaccggtg ggtacgggcg tcgaggattt ctggacgggg
     2941 ctgacgaccg gacgcaacgg ggtccgggag atcgcgcggt tcgccacgga cgacctcccc
     3001 gtgaaggttg cgggtgacgt ccacgacttc gacccgtcgc tctggctcga ccccaaggag
     3061 atccggcgga ccgatcgctt ctcgcattac gcgatcgccg cggggaagct cgcatgggag
     3121 gacgccggca cgcccgacgt gccctccgag cgagggggca tcgtcttcgg gaccgggatc
     3181 ggcgggatcg agacgctcct ggtccagcac agcgtgttgc tggagaaggg ccccgggcgc
     3241 gtgtccccgt tcatggtgcc ggccctgatg gccaacgcgg cggcgggtca cctcgcgatg
     3301 aagttcggcc tcaccggtcc gaactactgc accgtctccg cttgcgcgtc ctccaaccac
     3361 gcgatcgggg aagggatgcg gttgatccgc gacggctacc tggacgtctg catcaccgga
     3421 ggatcggagg ccgcgaccct cccgctcacg gtggccgcgt tcgcccagat gaccgcgctc
     3481 acgaagaacc ccgatcccga gagcgcgtcc cgtccgttcg acgcgaaccg caacggcttc
     3541 gtcctgtcgg agggggcggc ctcgctgata ctcgagtccg aggagcacgc gagggcgcga
     3601 ggcgcgcgga tctactgcga ggtcgcgggc tacgggatgt ccgacgacgc ccaccacatc
     3661 accgcgccgg atccgaaagg ctccggcgcg gcgctcgcga tgcggtgggc gctcgccgat
     3721 gccggcgagg agccggcgtc ggtccggtac gtgaacgcgc acgggacctc cacgccgctg
     3781 aacgacgcca gcgagaccgt cgcgatcaag gcggtgctcg gcgacgagat cgcccacgag
     3841 gtggcgatct cgtcgacgaa gtcgatgacc ggccacatgc tcggtgccgc gggggcggtc
     3901 gagggcgcgg tctgcgcact cgcgatccag cgtggcgtcg tcccacccac gatccactac
     3961 gagacgccgg atccggactg cgacctcgac tacgtcccga acgaggcccg cgagctggac
     4021 gtcacgctgg ccctgtcgaa ctccttcggg ttcggcggac agaacgcgtg cgtggcgttc
     4081 cgccgcgtcg cctgaggatc gagcccggtc actcctcgaa cgagcggaac caccgaccgc
     4141 cgttgcacac gaccaccgcc gtcatcccgc ggacgtcgcc ctcgaagcgg acgcggagcg
     4201 ggaccgcggg atcgccgacc gcgaacgacc cgtcgtcgag cgggatgagc tcgcccccct
     4261 cctcgtcgct cgcatcggta ggccaaagga tcgccagccg gcctttccgg aggacgacgc
     4321 ggagcaccgc gctccagggg tcgtcgttgc ggtagatccc cggatgccgg tcccaccccg
     4381 caggcggctc ctcaggatcg ggcccctcgt acgcctcgcc gcggaaccac gtgcctccgt
     4441 ggaacgcctc gacgaccttg cccgcttcat cgcgccggaa ctcgaagggg aaccgctcga
     4501 acgccgggtg cgcggccagg aaggactgac cgggatccgc gacgagggga tcccgctcga
     4561 ggcgagctga caccgagccg atcgagatcg acaggccgcc ctcgtccttc cggacctcga
     4621 gcgttcggcc gtcctcaccc gcgtacgtgc cgacgaagtc gtcccgcgcg gggatcgcgg
     4681 tggccgcggg cggagcccag ggcgccggga gctcgtcgcc cgccaggccc gcccgcaccg
     4741 ccgccaacgt ggctcccatc acaccgcgct tgtccccggc tccgttctgg aggatgacgc
     4801 acgccatccc ttcg
//
LOCUS       DASUVE010000019         3302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_197490, whole genome shotgun sequence.
ACCESSION   DASUVE010000019 DASUVE010000000
VERSION     DASUVE010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197490"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VF036_00420"
     CDS             complement(<1..617)
                     /locus_tag="VF036_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX6263721.1"
                     /translation="MTLAGCTLAVVLGLPLVALAGWPQFQGGPSHDGLSDGPTAPLEV
                     AWRHSDIAIEDADVTGGLSSPVVSDDGTIVVVGPHEVLGFDGEDGSEVFTAPRDLGPS
                     VTPAIGSGPDGPVVVFAEGFGDRGPATTASPTASPTASPSAPPGEEGDTGSHLVAIDL
                     RSGETVWPSPVPLEDVVRTPVTVDETGVYVGDFSGHVTAVELASG"
     gene            complement(695..1192)
                     /locus_tag="VF036_00425"
     CDS             complement(695..1192)
                     /locus_tag="VF036_00425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6263722.1"
                     /translation="MRKLLLWCAVWSAVFGIAVPAPAHASGDWSWPVVGPVIRAFDPP
                     DSPFGSGHRGIDIAAPVGSAVHAASTGTITFAGPVGGRLFVTVDHGAGLESTYSFLGS
                     IAVRRGDVVSQGQAIARSGTGHAGDIVPGLHFGVKLADTYVDPLEYLGPVEVWRFIRL
                     APLGS"
     gene            complement(1254..2111)
                     /gene="whiG"
                     /locus_tag="VF036_00430"
     CDS             complement(1254..2111)
                     /gene="whiG"
                     /locus_tag="VF036_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HEX6263723.1"
                     /translation="MATAKKVATKPAARARKTPAKNARAKTVTITAALDDELAIRWDV
                     FKRTADGDAREKLILHYAPLVKYVASRVASGLPSSVDQHDLVSYGMFGLIDALEKFEP
                     GRGNKFETYAIPRIKGAIIDELRAMDWVPRSVRFKARELEKAQADLEAMLKRQPTEEE
                     LADRLGMNRRELHEMVSQISFVSVLALDEVVSAGSDRGESVALIDTLADKGIDPTWGV
                     ESQETRGMLAAAINSLSEREKIVVTLYYFEGLTLAEIGEILGVTESRVCQIHTKAVGG
                     LRGQLSEFD"
     gene            complement(2113..3039)
                     /locus_tag="VF036_00435"
     CDS             complement(2113..3039)
                     /locus_tag="VF036_00435"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HEX6263724.1"
                     /translation="MPEGLPPTEFALLSDFEDHLALERRLSPNTVAAYRGDLEQLATF
                     LARNHSSLGDAPYPILRRFLAQQHTLGYARATIARRVGAIKTFYRWAVSVERVPADPS
                     LLLGRPKVVNRLPTVLRPSEAEVLAEAPPVDGEGDPPERAVALRDRATLELLYGSGLR
                     VGEVASLTVDRIDVRRGRVLVLGKGSKERDVPISEYAADALRDYLEAGRPQMASEGTR
                     QLFFNRRGKPFSERDIRSMVQRYGATLLPGRRVTPHTLRHSFATHLLEGGADIRAVQE
                     LLGHASVATTQRYTHVSRRRLFDAYERAHPRA"
BASE COUNT          525 a         1134 c         1165 g          478 t
ORIGIN      
        1 tcccccgagg ccagctcgac ggcggtgacg tgaccgctga agtcgccgac gtagacgccg
       61 gtctcgtcga ccgtgaccgg ggtgcggacg acgtcttcga gggggacggg cgaaggccac
      121 accgtctctc ccgaccggag atcgatcgcg acgaggtggg agccggtgtc accctcctcg
      181 cccggaggag ctgagggcga tgccgtgggg gacgcggtcg gagatgcggt ggtggccggg
      241 ccccgatcgc cgaacccctc tgcgaacacc acgacagggc cgtcggggcc cgatccgatc
      301 gccggtgtga ccgacggtcc gaggtcccgc ggcgccgtga agacctccga gccgtcctcg
      361 ccgtcgaagc cgaggacctc gtgcggcccg acgaccacga tggtgccgtc gtccgagacg
      421 acaggcgagg agagccctcc ggtcacgtcc gcatcctcga tcgcgatgtc gctgtggcgc
      481 caggccacct ccagcggggc agtgggtccg tcggaaagac cgtcgtgcga cggcccgccc
      541 tggaactgcg gccatccggc gagcgcgacg agcggcaagc cgagcaccac ggcgagggtg
      601 cacccggcca gggtcatcga acgtcggggc agacgggaac ggtgggcgac gcgcaaggcg
      661 cgtcatccta gccggtcgcc tgcgagggga cgcctcagga ccccagcggg gcgaggcgga
      721 tgaagcgcca gacctcgacc ggaccgaggt actcgagcgg gtcgacgtag gtgtcggcga
      781 gcttcacgcc gaagtgaagg ccgggaacga tgtcacccgc gtgccccgtc cccgagcggg
      841 cgatcgcctg cccctgtgac acgacatcgc ctcgtcggac cgcgatcgag cccaggaacg
      901 agtaggtcga ctcgaggccg gcaccgtgat ccacggtcac gaacagtcgc ccgcccaccg
      961 gcccggcgaa cgtgatcgtg cccgtggagg cagcatggac ggccgagccg acgggtgcgg
     1021 cgatgtcgat cccgcgatga cccgatccga acggagaatc gggcggatcg aaggcgcgga
     1081 tcacgggacc gacgaccggc caactccaat cccctgaggc gtgcgccggc gcaggcacgg
     1141 cgattccgaa gaccgcactc cacaccgcac accacagaag gagcttcctc acgcgcagga
     1201 cgctaggagg tccgggcggc gcgcgccgtg aagcgcgtca cacgccaggg aggtcagtcg
     1261 aactcggaga gttggccgcg gagcccgccg acggctttcg tgtggatctg gcacacccgc
     1321 gactcggtca cacccaagat ctccccgatc tcggcgagtg tcaggccctc gaagtagtac
     1381 agggtcacca cgatcttctc gcgctcggag agggagttga tcgccgcggc cagcatcccg
     1441 cgggtctcct ggctctccac gccccacgtc ggatcgatcc ccttgtcggc gagggtgtcg
     1501 atcagcgcga ccgactctcc gcggtcgctg ccggcgctca cgacctcgtc cagagcgagc
     1561 accgagacga aggagatctg cgacaccatc tcgtggagct cgcgacgatt catgccgagc
     1621 cggtcggcga gctcctcttc ggtcggctgc cgcttgagca tcgcctcgag gtccgcctgc
     1681 gccttctcca gctcccgggc cttgaagcgc acggaccggg gaacccagtc catcgcgcgg
     1741 agctcgtcga tgatcgcgcc cttgatccgt gggatcgcgt aggtctcgaa cttgttgccc
     1801 cgtccgggct cgaacttctc gagggcgtcg atcagcccga acatcccgta gctcacgagg
     1861 tcgtgctggt cgacgctcga gggcagcccg ctggcgacgc ggctcgcgac gtacttcacg
     1921 agcggcgcgt aatgcaggat cagcttctct cgtgcgtcac cgtcggccgt ccgcttgaag
     1981 acatcccaac ggatcgccaa ctcgtcgtcg agggcggcgg tgatcgtgac cgtcttggcc
     2041 cgcgcgttct tggcaggcgt cttccgggca cgggccgccg gcttcgtggc caccttcttc
     2101 gcagtcgcca tctcaggccc tcgggtgcgc gcgctcgtag gcatcgaaga gccggcgacg
     2161 cgagacgtgc gtgtagcgct gcgtcgtcgc gacgctcgcg tgtccgagca gttcctgcac
     2221 ggctctgatg tcggcccctc cttccagcag gtgcgtggcg aacgagtgcc ggagcgtgtg
     2281 tggggtgaca cgccttccgg gcagcagggt agcgccatac cgctgaacca tcgaacggat
     2341 atcgcgctca ctgaagggtt tcccacgcct gttgaagaag agctgacgtg ttccctcgga
     2401 ggccatctgg ggccgtccgg cctccagata gtcgcgcaga gcgtcggcag catattcgga
     2461 gatggggacg tctcgctcct tcgagccctt ccccagcacc agcacgcggc ctcgacgcac
     2521 atcgatccgg tcgacggtga ggctcgcgac ctctccgacc cggagaccgg agccgtagag
     2581 gagctcgagg gtggcccgat cccggagggc cacggcccgc tccggtggat cgccctcccc
     2641 gtcgacgggc ggcgcctccg cgagcacctc ggcctcgctc ggcctgagga cggtcggaag
     2701 gcgattcacg accttcgggc gtccgaggag cagggatgga tcggcgggga cccgctcgac
     2761 cgagaccgcc caccgataga acgtcttgat ggctccgacg cggcgcgcga tcgtcgcccg
     2821 cgcatacccg agcgtgtgct gttgcgcgag gaaccggcga aggatcgggt acggggcgtc
     2881 gcccagggag ctgtggttcc gggcgaggaa cgtggcgagc tgctccagat cgccccggta
     2941 ggccgcgacg gtgttcggcg acaggcgacg ctcgagggcc agatggtcct cgaagtcgct
     3001 gagcagggcg aactcggttg ggggaagtcc ttcgggcatg cacgctcccg gggtcgctgc
     3061 ctgtgtatca accggaaggg tgcgacgcaa gcccccacac ggggggtgcg gcggtcaccc
     3121 tgtcggccgg aaggcccacc gtcaacgggg cgacggttcc gcgcccgcga cctgcaccgt
     3181 ggctcgatgg cgtcgctcga atcggccgcc ggagctcatc accaggcccc gcagctcgag
     3241 ccggacgagg gtcgtgatcg cgtccggcac tgacatccgg gcacgatggg cgacggcgtc
     3301 gg
//
LOCUS       DASUVE010000020         3465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807 k141_9062,
            whole genome shotgun sequence.
ACCESSION   DASUVE010000020 DASUVE010000000
VERSION     DASUVE010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9062"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            394..2013
                     /locus_tag="VF036_00440"
     CDS             394..2013
                     /locus_tag="VF036_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6263725.1"
                     /translation="MVGYDAIVIGGGHNGLTAAFYLARAGLRTIVLERRELVGGACVT
                     EEIAPGCRASTTSYIASMLRPEVIRDLRLEHHGLRMVPCEPGLQAVFEDGTVVPWWTD
                     HDRAVEEFASTISARDAESFDRVHRRLHALARYLQPFFLEEPPNLYARGWAKVREGSR
                     AWRRFRSISGDELADLVRFTTSSLGEFVERHFESDRMRRMYLANNVYGMHAPPYMPGT
                     AIGLLFHLLSGGSEGVQGFYGHVIGGMGSITHAMAEAVRAAGGEIRTAALVSNVIVRD
                     GRATGVALASGEELSAGVVLSNADPKRTFLELVDPGELPEDFRDDVSAIKMAGPCAKV
                     NFVLSAEPTWTGMPADADANRRSLATLVPTLAEAQAIYDRHRDGEIPDRLWVDCVTAS
                     NVDDSLAPPGVHVMTAFVQYVPFALRRGTWDERRDELLSTVVGRIEESAPGFRGTIEA
                     AQVLTPLDLERTYGLTEGNIFHGDLHIGQLFSMRPTPSWSRYRTPVRGLYLCGAGAHP
                     GGGVTGAPGYGASHAALRDGRPRRGRRRPWR"
     gene            2004..2231
                     /locus_tag="VF036_00445"
     CDS             2004..2231
                     /locus_tag="VF036_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEX6263726.1"
                     /translation="MALKVPCPNCGWRPSTEFAWGGELRPIDAPDAESDFARVFLPAN
                     AAGPQEERWFHLLGCRRWLTVTRDTTTNEIG"
     gene            2232..>3465
                     /locus_tag="VF036_00450"
     CDS             2232..>3465
                     /locus_tag="VF036_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX6263727.1"
                     /translation="MSGTLRLGRRRIRVEDGDSVASALYRAGIRTFSRSLKYHRRRGL
                     YCGTGECPNCLVTVDGIPGVRSCVTPAADGMRVRRERGWPSVERDAFAILDRLHVLLP
                     VGFYYKTFIRPRWSWAVASRMIRRAVGLGRLPGGPVVTRTNRHLHCDVLVVGAGESGR
                     AAASSAAIRGERVVLCDEGDVAERPDGVEVLARHAAIGVYEGPMAALASAEGIVQVHP
                     RRVVAATGSSEVHAVFPGNDLPGVMLGRAAEGLAERGIAPGRRAVVVAGHDEGIEHLT
                     MLQDAGVSIAAVVVPSSLARQVPDDVWTLIDATVVRADGKDRVRFAVLRDADGVTRGI
                     ACDLFVLSVGLSPRDDLARMSAAGEVEPVGDAGAEASPMAMTHAAGTVCLCEDVSQGD
                     LERAWAEGFRSSELLKRYT"
BASE COUNT          480 a         1140 c         1293 g          552 t
ORIGIN      
        1 tgatctcgcg acccaggtgc tctggagaag tgcgaaggcg aatgtcctct ctgcgagagg
       61 cgagcacgat ggcgccatgg tcctggcgcg agcggcggtt gccctcgccg agggaaccga
      121 cgacacgaat atgtgcgcgg acagtctcat ggatctcgct ctggtgctgt ccgcgaccgg
      181 cgactctgcg agcgtgtcga ggcgatacgg cgcgctcggg agctgtatag ccgcaagggg
      241 aacgtcgtat ccgccgccat cgccgagcag ctggaaagaa cggcaagctg agcagaaccg
      301 cgacggtgaa ccacggtcac cttcggatcg gcgccttccc aacctgctcc gtagagttcc
      361 tgccggttcg gagacgctcg aggagaggga cgcgtggtgg gctacgacgc gatcgtgatc
      421 ggtggcggac acaacgggct gacggccgcg ttctacctcg cccgcgccgg tctccggacg
      481 atcgtgctgg aacggcggga gctcgtcggc ggcgcgtgcg tcacggagga gatcgcgccg
      541 ggctgtcgcg cgtcgaccac gtcgtatatc gcgtcgatgc tccgtccgga ggtgatccgc
      601 gatctccggc tcgagcacca cggactgcgg atggtcccgt gcgagccggg gctgcaggcc
      661 gtcttcgagg acgggacggt cgtcccgtgg tggaccgatc acgaccgggc ggtggaggag
      721 ttcgcgtcca ccatctccgc ccgcgacgcg gagtcgttcg accgggtcca tcgccggctc
      781 catgcgctgg cccgctacct ccagccgttc ttcctcgaag agccgccgaa cctctacgcg
      841 cgcgggtggg cgaaggtgcg cgaggggagc cgcgcgtggc ggcggttccg atcgatcagc
      901 ggcgacgagc tcgccgatct ggtccggttc acgacgagct ccctcggcga gttcgtcgag
      961 cgacacttcg agtccgatcg catgcgccgg atgtacctcg cgaacaacgt ctacgggatg
     1021 cacgcgcctc cgtacatgcc cggcacggcg atcgggctcc ttttccatct cctgtcgggc
     1081 ggcagcgagg gggtgcaggg gttctacggg cacgtgatcg gcggcatggg gtcgatcacc
     1141 cacgccatgg ccgaggcggt ccgcgcggcg ggcggcgaga tccggacggc cgctctcgtg
     1201 tcgaacgtga tcgtgcggga cggccgcgcg accggggtgg cgctcgcgag cggtgaggag
     1261 ctctccgccg gcgtcgtgtt gtcgaacgcg gatccgaagc gaacgttcct cgaactggtc
     1321 gatcccggag agctccccga ggacttccgc gacgacgtgt ccgcgatcaa gatggccggg
     1381 ccgtgcgcga aagtgaactt cgtcctctcg gccgagccga cgtggacggg gatgcccgcg
     1441 gacgccgacg ccaaccgccg atccctcgcc acgctcgtcc cgacgctcgc ggaggcccaa
     1501 gccatctacg accgtcatcg cgacggcgag atccccgacc gtctctgggt ggactgcgtc
     1561 acggcttcga acgtcgacga ttcgctggcg ccacccggtg tgcacgtgat gaccgcgttc
     1621 gtgcagtacg tcccgttcgc gctccgccgg ggaacgtggg atgagcgtcg cgacgagctt
     1681 ctgagcaccg tggtcgggag gatcgaggag tccgcaccgg gtttccgcgg cacgatcgag
     1741 gccgcgcagg tgctcactcc gttggatctg gagcgcacgt acgggctgac cgagggcaac
     1801 atcttccacg gcgacctcca catcgggcag ctcttctcga tgcggcccac gccgtcatgg
     1861 tcgcgctacc gcacgccggt ccgaggcctc tatctgtgcg gggcaggcgc gcatccggga
     1921 ggtggagtga ccggagctcc gggctacggc gcatcccacg cggcgctgcg cgacggtcgg
     1981 ccccggagag gacggagacg tccgtggcgt tgaaggttcc gtgcccgaac tgcgggtggc
     2041 gtccctcgac ggagttcgcg tggggcggtg agctccggcc gatcgacgcc cccgatgcgg
     2101 agtcggactt cgcgcgggtc ttcctgccgg ccaatgccgc cggcccccag gaggagcgat
     2161 ggttccatct cctcggttgc cggcggtggc tcaccgtgac ccgcgatacg acgacgaacg
     2221 agatcggcta gatgtcgggc acgctgcggc tcggccggag gcggatcagg gtcgaggacg
     2281 gggactcggt cgcgtcggcg ctgtatcgcg ccgggatcag aacgttctcg cgatcgctca
     2341 agtaccaccg gcgccgaggc ctgtactgcg ggacggggga gtgtcccaac tgcctcgtga
     2401 ccgtcgacgg catcccgggc gtccgttcgt gcgtgacgcc ggccgccgac gggatgcggg
     2461 tgcgccgcga gcgcgggtgg ccgtcggtcg agcgggacgc attcgcgatc ctcgatcgac
     2521 tgcatgtgct gctccccgtc ggcttctact acaagacgtt catccgtccg cgctggtcgt
     2581 gggcggtggc gtctcggatg atccggcgcg ccgttgggct gggacgtctg cccggcgggc
     2641 cggtcgtgac ccggacgaac cgacatctgc actgcgacgt tctcgtcgta ggcgcgggcg
     2701 agtcgggtcg cgccgccgcc tcctccgctg cgatccgtgg cgagcgggtg gtcctctgcg
     2761 acgagggcga cgtggccgaa cgtcccgacg gggtcgaggt cctcgcccgc cacgccgcga
     2821 ttggcgtcta cgagggaccg atggcggcgc tcgcgtcggc ggagggcatc gtccaggtgc
     2881 acccccgacg ggtcgtggcg gcgaccggca gctcggaggt ccacgcggtc ttcccgggga
     2941 acgatctgcc tggggtgatg ctcggtcggg cggccgaggg gctcgcggag cggggtatcg
     3001 cgccgggacg tcgggcggtg gtcgtcgccg gccacgacga agggatcgag cacctgacca
     3061 tgctccagga cgccggcgtc tcgatcgcgg ccgtggtcgt cccctcgtcg ctcgccaggc
     3121 aggtgccgga tgatgtctgg accctgatcg atgcgacggt cgtgcgtgcg gacgggaagg
     3181 accgggttcg cttcgcggtg ctccgggatg ccgacggcgt gacccggggg atcgcctgcg
     3241 atctgttcgt cctctcggtg ggtctgtccc cgagggacga cctcgcccgg atgagcgccg
     3301 ccggagaggt cgagcccgtc ggcgacgccg gggcggaggc atcgccgatg gccatgacgc
     3361 acgccgccgg gaccgtatgc ctgtgcgagg acgtgtccca gggggacctg gagcgcgcgt
     3421 gggcggaggg gttccgttcg tcggaactcc tgaagcggta cacga
//
LOCUS       DASUVE010000021         3427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_198556, whole genome shotgun sequence.
ACCESSION   DASUVE010000021 DASUVE010000000
VERSION     DASUVE010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198556"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1805
                     /gene="lon"
                     /locus_tag="VF036_00455"
     CDS             <1..1805
                     /gene="lon"
                     /locus_tag="VF036_00455"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007238601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX6263728.1"
                     /translation="AKETLAEVQVRERIRDEVAGSLEERQREMLLREQMAAIRKELGE
                     DGTEDVLEEYRTKIADAGMPEDVRSQADRELGRLERTSEQSPEYGWIRTYLDWLLDVP
                     WSSKTEDNLDIGSAREVLDADHEGLRDVKDRILEYLAVRKLRSERGLAETGGRGSGAI
                     LTLVGPPGVGKTSLGESVARALGRNFVRVSLGGVHDESEIRGHRRTYVGALPGRIVRA
                     LKDAGTKNPVMMLDEVDKVGADWRGDPSSALLEVLDPAQNHTFRDHYLDVDLDLSEVL
                     FIATANVAETIAGPLLDRMEVIRIDGYTEDEKVAIARRHLLPRQVERNGLRPEEVEVT
                     DDALRGVVGDYTREAGVRNLERELGKIVRKVATKLTAGEGGSTARVDAADLREYLGRQ
                     RFFFEAAERTAVPGVATGLAVTGTGGDVLFIEATREEVPETGMGDGLTLTGQLGDVMK
                     ESARIALSYVRSHAEALDISADRLRGRFHVHVPAGAVPKDGPSAGVAMTTALASLLTG
                     RPVRAEVGMTGEVTLQGRVLPIGGVKQKVLAAHRAGLKEVILPARNEGDLDDVPEQVR
                     EEMTFHLAEAVDDVLAIGLGSVADDAIEDPSRVA"
     gene            1886..3196
                     /locus_tag="VF036_00460"
     CDS             1886..3196
                     /locus_tag="VF036_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6263729.1"
                     /translation="MTEHGTLEHELRGTDEAALHEFLRLVVPLAAVFAVIQAVAGGVL
                     RDGSMLLVGLLIALYGGCLIYARELFDRDRVQASAGWISGGLIVPSLGTVILQPSMLP
                     TIVLLPLTALAVVLPFIRAPRLHLVLGLTLAWVIAIGVVGAVAPPPVDPAPWYGKGFQ
                     VVTMAAATFLTILLFTRYRARLLLAAHREREVSLKLREVDRLKTTLMRAASHDLRTPT
                     ATILGAAETLRHLSLDEAEARALLAAISRGARRLSGLLDDLLDAESLQAGVVELSAEP
                     ADVGEVVRDAVASLEIPAEMDLRLDLQPARATVDRARVARIVENLVTNAIRHAPGSGV
                     VCVLVRPHESGAEIVVEDQGPGVPEADRFTIFAPFRRGDTDAPGTGLGLAIVEGFVIL
                     HGGRVWVEDRPGGGASFHVSLPGVGQGVAATARPELVVDGIRAD"
BASE COUNT          482 a         1179 c         1260 g          506 t
ORIGIN      
        1 gggcgaagga gacgctcgcc gaggtccagg tgcgcgagcg gatccgcgac gaggtcgccg
       61 gcagcctcga ggagcgccag cgcgagatgc tcctccgcga gcagatggcg gcgatccgca
      121 aggagctcgg cgaggacggc accgaagacg tcctcgagga gtaccggacc aagatcgcgg
      181 acgccgggat gccggaggac gtgcgctcgc aggcggatcg cgagctcggc cggctcgagc
      241 ggacgtccga gcaatcgccc gagtacggat ggatccgcac ctacctcgac tggctgctcg
      301 acgtcccgtg gtcctcgaag accgaggaca acctggatat cgggtcggcg cgcgaggtgt
      361 tggacgcgga ccacgagggg ctccgcgacg tgaaggaccg catcctcgag tacctcgcgg
      421 tccgcaagct gcggagcgag cgggggctcg ccgagaccgg tggtcgtggc tccggtgcga
      481 tcctcacgct ggtcggccct ccgggcgtcg gcaagacgtc gctcggggag tcggtcgcgc
      541 gcgccctcgg gcgcaacttc gtccgggtct cgctcggcgg cgtccacgac gagtccgaga
      601 tccgtggcca ccgtcggacc tacgtggggg cgctgccggg acggatcgtg cgtgcgttga
      661 aggacgcggg cacgaagaac cccgtgatga tgctggacga ggtcgacaag gtcggcgccg
      721 actggcgagg cgacccctcg agcgcgctcc tcgaggtcct cgatcccgcg cagaaccaca
      781 cgttccgcga ccactacctg gacgtcgacc tcgatctgtc cgaggtgctg ttcatcgcga
      841 ccgccaacgt cgcggagacg atcgccggcc cgctcctcga ccggatggag gtcatccgga
      901 tcgacggcta caccgaagac gagaaggtcg cgatcgcccg gcggcacctg ctgccccgcc
      961 aggtcgagcg caacggcctg cgccccgagg aggtcgaggt gaccgacgac gccttgcgag
     1021 gggtggtcgg cgactacacg cgcgaggcgg gtgtccgcaa cctcgagcgc gagctcggca
     1081 agatcgtgcg gaaggtggcg accaagctca ccgcgggcga gggcggatcg actgctcgcg
     1141 tggacgcggc cgatctccgc gagtacctcg gccggcagag gttcttcttc gaagcagcgg
     1201 agcggaccgc cgtccccggc gtggccacgg gcttggccgt caccgggacc ggcggcgacg
     1261 tgctgttcat cgaggcaacc cgcgaggaag tcccggagac ggggatgggg gacgggctga
     1321 cgctcaccgg acagctgggc gacgtgatga aggaatcggc ccggatcgcg ctctcgtacg
     1381 tgcgctcgca cgccgaggcc ctcgacatct cggcggaccg gctccgcggt cggttccatg
     1441 tgcacgtccc ggcgggcgcc gtccccaagg acggccccag cgcgggggtc gcgatgacga
     1501 cggcgttggc ttcgctgctg acgggccgac cggttcgcgc cgaggtcggc atgaccggcg
     1561 aggtgacgct ccaagggcgc gtgctcccga tcggtggggt caagcagaag gtccttgccg
     1621 cgcaccgggc gggcctgaag gaggtcatcc ttcccgcgcg caacgaggga gacctcgacg
     1681 acgtgccgga gcaggtgcgc gaagagatga cgttccacct ggcggaagcg gtcgacgacg
     1741 tgctcgcgat cgggctcggc tccgtggccg acgacgcgat cgaggatccc tcccgggtcg
     1801 cgtaagggac ggggtcaggt cgccctcggg agcaaccgat acacaaccat aggtatcggt
     1861 tcccactccc cgggaagggc gcgcgttgac ggaacacggc acgctcgaac acgagctccg
     1921 cgggacggat gaggcggcgc tgcacgagtt cctccggctc gtggtcccgc tcgcggcggt
     1981 gttcgccgtc atccaggcag tcgccggagg cgtgctgcgc gatgggtcga tgctcctcgt
     2041 cgggctcctg atcgccctgt acggagggtg cctgatctac gcccgcgagc tcttcgatcg
     2101 ggatcgggtc caggcgagcg cggggtggat cagcggtggg ctgatcgtgc cgtcgctcgg
     2161 cacggtgatc ctgcagcctt cgatgctccc cacgatcgtc ctgttgccgc tgacggcgct
     2221 cgccgtggtg ctcccgttca tccgagcccc ccggctccac ctggtactcg gcctcacgct
     2281 cgcgtgggtg atcgccatcg gcgtcgtcgg cgctgtcgcg ccacctcccg tcgaccccgc
     2341 cccgtggtac gggaaggggt tccaggtcgt cacgatggcg gcagccacgt tcctgacgat
     2401 cctcctcttc acgcgctacc gggcacggct cctgctggcg gcccaccggg agcgagaggt
     2461 ttccctcaag ctccgcgagg tcgaccgttt gaagacgacg ctgatgcgcg cggcgtcgca
     2521 cgatctccgg acgccgacgg ccacgatcct cggagcggcg gagacgctcc ggcacctctc
     2581 gctcgacgag gccgaggccc gggcgctcct cgcggcgatc tcccgcggcg cgcgccggct
     2641 ctccgggctc ctcgacgatc tgctcgacgc ggagagcctc caggcgggcg tggtcgagct
     2701 ctcggccgag ccggccgacg tcggcgaggt ggtgcgcgac gccgtcgcct cgctcgagat
     2761 ccccgccgag atggacctgc gcctcgatct ccagccggcc cgcgcgacgg tcgaccgcgc
     2821 tcgcgtcgca cggatcgtcg agaacctcgt gacgaacgcg atccggcacg ctccgggctc
     2881 gggggtcgtg tgtgtgctcg tgcgacccca cgagagcggt gccgagatcg tcgtcgagga
     2941 tcaggggccc ggtgttcccg aggccgatcg gttcacgatc ttcgctccgt tccgcagggg
     3001 tgacacggac gcccccggca ccgggctggg cttggccatc gtggaggggt tcgtcatcct
     3061 gcacggcggc cgggtgtggg tcgaggaccg gccgggcggg ggagcgtcgt tccacgtgtc
     3121 cctccccggg gtcggccagg gagtggcggc caccgcacga cccgagctcg tcgtcgacgg
     3181 gatccgcgcc gactgagtcc gtccttcggc acgcttgcgg tctcttgccg tcgtctccga
     3241 cctcgccatg ccccgacctg tcgggcgaag tggttccgga cagaacgctc acgtacggtc
     3301 ggccgaatga cgagaggagt gagtcggccg gggagccggg tggtcgcctc cctcgggctg
     3361 tgcgtcgccc tcgcgggatg tggcggtggg tctgccccgg actcctcccc gcctccatcc
     3421 gcgacgg
//
LOCUS       DASUVE010000022         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_388212, whole genome shotgun sequence.
ACCESSION   DASUVE010000022 DASUVE010000000
VERSION     DASUVE010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388212"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VF036_00465"
     CDS             <1..885
                     /locus_tag="VF036_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263730.1"
                     /translation="SVVKIDAETGRILSRIPIGAAGRDEGNLAFIDHPIVAGEGGVWL
                     LRPSKLLHLDPVHEDIRSRIEVGVATSQTVDVGFDAIWVLTDRTLYRVHPGTDQARPL
                     LRLPPAAGIATYALAIGEHIWVVDSDGTLVRMDPITGSQEQTETDLSTGRIAATDAGL
                     WVADILSGTMTFLDAETLRETGDPVEISGSVDQVITFDQDLWVLDNLAGTVKRVNTLS
                     REEGRPTRVGQDPTDMSAGLGAVWVGDRDGSLYRLDTVTLDVQTFPIGAEVLGVAVDE
                     RAEAVWVYVGAPVTGAAP"
     gene            complement(882..1301)
                     /locus_tag="VF036_00470"
     CDS             complement(882..1301)
                     /locus_tag="VF036_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263731.1"
                     /translation="MASRLHRLVIVSAAAALALTAGSCGGGGGIGVTLADFTITLDES
                     SAAAGDLTFDVSNDAEQTHEFVIFKTDLAEDQLPTDEDGNVDETGGGVELIDEIEDIE
                     AGANQSLTATLDAGSYVFICNLPGHYGQGMHTSFTAS"
     gene            1484..2149
                     /locus_tag="VF036_00475"
     CDS             1484..2149
                     /locus_tag="VF036_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6263732.1"
                     /translation="MARVLVVDDEPRIVSFVSRALSAEGFQVDGAHDGIRALELAKTG
                     RYELVVLDLLLPELNGITVLRDLLESRPDQRVLVLSALSDVETKVRCLEFGASDYLSK
                     PFSLSELIARVRARLRQPSVGPRHRILRGGALTLDLTRRVVETKGSHITLSEREFLLL
                     EYLMRQDGEVCSRERLLSEVWGYSFDPGSNLVDVCVGRLRSKLGSDVIETVRNVGYRF
                     NAA"
     gene            2163..>2696
                     /locus_tag="VF036_00480"
     CDS             2163..>2696
                     /locus_tag="VF036_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX6263733.1"
                     /translation="MTWVAFALANMAVIVLLSRWETIPFHFVWVSLTLVYGLRLWRLR
                     ATMVVLLVVSGATGAALAVSIARGHEGPDELAEVPLMAAMFVAMVWHAVRRQAAVEEA
                     GRLAESEHRLLMGQREFVRDASHELRTPITVARGHAELIRQSGPNGQVSKDVDVILDE
                     LGHLSRLSERLLILTTAG"
BASE COUNT          413 a          890 c          918 g          475 t
ORIGIN      
        1 tcggtggtga agatcgacgc cgagacgggc cggatcctgt cccggatccc catcggcgcc
       61 gccgggaggg acgaggggaa cctcgccttc atcgatcatc cgatcgtggc gggcgaagga
      121 ggggtgtggc tgctccgtcc gtccaagctg ttgcatctcg atccggtcca cgaggacatc
      181 cgttcgagga tcgaggtcgg cgttgccacc tcccagaccg tcgacgtggg tttcgacgcg
      241 atctgggtcc tcaccgatcg gacgctctac cgcgtgcacc ccgggacgga tcaggcacga
      301 ccgctcctcc gcctgccgcc cgccgccggc atcgcgacgt acgcactcgc gatcggcgag
      361 cacatctggg tcgtggacag cgacgggacc ctcgtgcgga tggatccgat caccgggtct
      421 caggagcaga ccgagacgga tctgtcgacc gggcggatcg ccgccacgga cgccggcctg
      481 tgggtcgccg acatcctctc gggcaccatg accttcctgg acgccgagac gctccgggaa
      541 acgggtgacc cggtcgagat ctccgggagc gtcgatcagg tcatcacgtt cgaccaggat
      601 ttgtgggtgc tggacaacct cgccggcacc gtcaagcggg tgaacaccct ctcccgagag
      661 gagggacgtc ccactcgggt cggtcaggac cccaccgaca tgagcgccgg cctcggagcc
      721 gtgtgggtgg gcgaccgcga tggctcgctc tatcgcctgg acaccgtcac gctcgacgtg
      781 cagacgttcc cgatcggagc ggaggtgctg ggtgtcgccg tcgacgaacg cgcggaagcg
      841 gtgtgggtct acgtcggtgc tcccgtcaca ggcgccgccc cctaggacgc cgtgaacgac
      901 gtgtgcatcc cctggccgta gtggccgggc aggttgcaga tgaagacgta gctcccggcg
      961 tcgagcgtcg cggtcaacga ctggttcgcg ccagcctcga tgtcctcgat ctcgtcgatc
     1021 agctcgacgc ccccgcccgt ctcgtcgacg ttcccgtcct cgtccgtggg gagctgatcc
     1081 tccgcgaggt ccgtcttgaa gatcacgaat tcgtgggtct gctccgcgtc attcgagaca
     1141 tcgaacgtga gatcgccggc ggcggcgctc gactcgtcca aggtgatcgt gaagtcggcg
     1201 agggtgaccc cgatcccgcc tccgcctccg caactgccgg ccgtcaacgc gagtgcggcg
     1261 gccgccgata cgatcaccag ccgatgcaga cgtgaagcca cgattctccc ccttcctctc
     1321 ggcgttcctc cggcctcctg tcgacagcga gcctgaccga cacgcgggac tgtagccacg
     1381 agagcatccg aggcagaagc tcgtgacgcc accgtcagcg aaccgtcatc ccgtggtcat
     1441 gtagggggtt tcgcgctccg tcgtcgaggc ctagcatcgg tccgtggcca gggtgttggt
     1501 cgtcgacgac gaaccgagga tcgtcagctt cgtctcgcga gcgctctccg cggaagggtt
     1561 ccaggtggat ggcgctcacg acggcatccg tgcgctcgag ctggccaaga ccgggaggta
     1621 cgagctggtg gtgctcgacc tgctcctgcc agagctgaat gggatcaccg tccttcggga
     1681 tctgttagag agccgtcccg atcagcgcgt cctcgtcctc tctgcgctct ccgacgtcga
     1741 gacgaaggtc cggtgcctgg agttcggagc ttcggattac ctgtcgaagc cgttctccct
     1801 ctccgagctg atcgcgcgcg tgcgcgcccg gctccggcag ccgagcgtcg gcccgcgcca
     1861 tcggatcctc cgcgggggag ctctgacgct cgatctgacc cgtcgcgttg tagagacgaa
     1921 gggcagtcac atcacgctga gcgaacggga gttcctcctc ctggaatacc tgatgcgcca
     1981 agacggcgaa gtctgcagcc gggagcgtct cctttccgag gtatggggct actcgttcga
     2041 tccgggcagc aacttggtag acgtctgcgt cgggcggctc cgatccaagc tcggtagcga
     2101 cgtgatcgag acggtgcgga atgtcgggta tcggttcaac gctgcgtagg tactgggtcg
     2161 aggtgacgtg ggtcgccttc gcgctggcga acatggccgt catcgtgctc ctcagccgct
     2221 gggagacgat cccgttccac ttcgtctggg tgagcctgac actcgtgtac ggcctgcggc
     2281 tgtggcgcct ccgcgccacg atggtcgtgc tgctggtcgt gagcggggcg accggcgcgg
     2341 cgctcgccgt gtcgatcgcc cgtggccacg aggggcccga tgagctcgcg gaagttcccc
     2401 tgatggcggc gatgttcgtc gcgatggtgt ggcacgccgt ccggaggcag gcggcggtcg
     2461 aagaggcggg gaggttggcc gagtccgagc atcgcctcct gatgggacag cgagagttcg
     2521 tgcgcgacgc gtcccacgag ctccggacgc cgatcacggt agcgcgcggc cacgcggagc
     2581 tgatccgcca gtccgggccg aacggtcagg tctcgaagga cgtcgacgtc atcctcgacg
     2641 aactcgggca tctatcgagg ctctccgagc ggctactcat actcacgacg gccggt
//
LOCUS       DASUVE010000023         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_201130, whole genome shotgun sequence.
ACCESSION   DASUVE010000023 DASUVE010000000
VERSION     DASUVE010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201130"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /locus_tag="VF036_00485"
     CDS             complement(<1..1456)
                     /locus_tag="VF036_00485"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HEX6263734.1"
                     /translation="MLSVPVQTTLEEGVPLGEVDFVVVDLETTGGSPRDSRITEIGAV
                     RVLGGERIGSFHALVNPGEAIPPFISHLTGIDDLLVREEPPIEAVLPAFLEFARGAIF
                     VAHNARFDFSFLNENLRRLEYPLLEGPPVCTAKLARRVVWPEVPNVRLQTLARYFRTR
                     VQPIHRALDDAMACAEVLDQLLDLGARLGIVTLGDLHQAVTARGRPHYGKIRLADDLP
                     RAAGVYRFVGRDDRVLYVGKARDLRARVKSYFYGDERKKVENLLGEVHRVEGEETPGG
                     ELEALVLEARLIRRHEPKYNRRGTTWRRFAYLKLDPTEAFPRLKVVHRAGADDGCAYL
                     GPFSGSGAARLAKEALEEIIPLRRCTRSMGARTRFSPCALAGIGRCPSPCDGGIDTER
                     YGELVRDLLSSLESPDGLLGALEARMRRLADAERFAEAASVRDRLRALASALLRSRQD
                     AWLVGAGHLEVVAEGERPLAFVGGALARADDTDVQ"
     gene            complement(1585..>2090)
                     /locus_tag="VF036_00490"
     CDS             complement(1585..>2090)
                     /locus_tag="VF036_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019500309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263735.1"
                     /translation="DAAGTLTIDAGDTGAVIEVVVNGDATYEGDETLTVTLSDPSGAV
                     LVDGAATGTIRNDDKAPTTVTVRIARTPTKIVASGIMEHAHAGLRITATLFRKAHGRF
                     VKVAAKTARVRDLRDRDGDGKPDGAYAATFLRPQAKGLYKVVVRFKGSAAYRPCTRSK
                     IFRLPAA"
BASE COUNT          307 a          712 c          766 g          305 t
ORIGIN      
        1 gctgtacgtc cgtgtcgtcc gcccgcgcga gcgcaccacc cacgaaggcc aatggccgct
       61 cgccttcggc cacgacctcg aggtggccag ctccgacgag ccaggcgtcc tggcgcgacc
      121 ggagcaacgc cgaggcgagg gcgcggaggc ggtcgcggac ggaggcggcc tctgcgaatc
      181 gctcggcgtc cgccagccgg cgcatccgtg cttcgagcgc tccgaggagc ccgtccgggg
      241 actcgaggga ggagaggaga tcccggacga gctctccgta gcgctcggtg tcgatcccgc
      301 cgtcacacgg ggagggacat cggccgatgc cggcgagggc gcaggggctg aaccgggtgc
      361 gtgcgcccat cgagcgggta cagcgacgga gcgggatgat ctcctcgagc gcctccttcg
      421 ccaaccgggc cgcccccgac cccgagaagg gtccgaggta cgcgcagccg tcgtcggccc
      481 cggcccgatg gacgaccttg agccggggga acgcctcggt cggatcgagc ttcaggtagg
      541 cgaaccgacg ccatgtcgtc ccgcgccggt tgtacttggg ctcgtggcgt cggatcagcc
      601 tcgcctcgag gaccagagcc tcgagctcgc cgcccggcgt ttcctcaccc tcgacgcggt
      661 gaacctcgcc caggaggttc tccaccttct tgcgctcgtc cccgtagaag taggacttca
      721 cgcgcgcacg gaggtcccgt gccttgccga cgtagaggac gcggtcgtcc cgaccgacga
      781 agcggtagac gccggccgcg cgggggaggt cgtcggcgag gcggatcttg ccgtaatgcg
      841 ggcgtccgcg tgccgtgacc gcctggtgga gatcgccgag cgtcacgatc cccaggcggg
      901 cgccgaggtc gaggagctga tcgaggacct cggcgcaggc catggcgtcg tcgagcgctc
      961 ggtggatcgg ctgcacccgg gtgcggaagt agcgagcgag cgtctgcagc cggacgttcg
     1021 gcacctccgg ccagacgacc cggcgggcga gcttcgccgt gcagacaggc ggtccctcca
     1081 gcagggggta ctcgagccga cggaggttct cgttcaggaa cgagaagtcg aaccgcgcgt
     1141 tatgggcaac gaagatcgcc ccccgggcga actcgaggaa cgcggggagc accgcttcga
     1201 tcgggggctc ctcgcggacg aggagatcgt cgatgcccgt caggtgcgag atgaacggcg
     1261 ggatggcctc ccccgggttc acgagtgcgt ggaagctgcc gatccgctct ccgccgagca
     1321 cacgcaccgc gccgatctcg gtgatcctcg agtcccgagg ggagccaccg gtggtctcga
     1381 ggtccaccac cacgaagtcg acctccccga gcggcacgcc ctcctcgagc gtcgtctgga
     1441 cgggtaccga gagcacgggc gcctccgatc ggtgggccga cgggaggctc accgtaccga
     1501 acaggtgttc gatggtcaag gatcgggcct gcgcgtgcgg cctcgcggaa ccatcctgga
     1561 gagcggtgcc gacatcggtt cgggctacgc cgccggaaga cggaagatct tcgaccgggt
     1621 gcagggcctg taggccgccg agcccttgaa gcggaccacg actttgtaga gccccttcgc
     1681 ctgagggcgg aggaacgtcg cggcgtacgc gccgtccggc ttgccgtcgc cgtcgcggtc
     1741 gcggaggtct cggacgcgag ccgtcttcgc cgcgaccttc acgaaccgac cgtgcgcctt
     1801 ccggaacagg gttgccgtga tccggagtcc cgcgtgcgcg tgctccatga tcccggaggc
     1861 gacgatcttc gtcggtgtgc gcgcgatgcg gacggtgacg gtggtgggcg ctttgtcgtc
     1921 gttgcggatc gtgccggtgg ccgcgccatc caccaggacc gcgcccgacg gatcgctcag
     1981 cgtcacggtc agggtctcgt caccctcgta ggtggcgtcg ccgttcacga cgacctcgat
     2041 cacggcgccg gtgtcgcccg cgtcgatcgt cagggtgccg gcggcgtcgg
//
LOCUS       DASUVE010000024         2819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1331245, whole genome shotgun sequence.
ACCESSION   DASUVE010000024 DASUVE010000000
VERSION     DASUVE010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331245"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..732
                     /locus_tag="VF036_00495"
     CDS             145..732
                     /locus_tag="VF036_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX6263736.1"
                     /translation="MRRGTLLCTIVVLAAVVGMPGVASAGGGCHGGVTQTDATGQADA
                     TVQLVDACFSATVTSVDPGTPVTFVNMDEFVHNVGGNQWGHFDDLHGGDTFRVSFEEA
                     GTYPFACSYHPGMTGAIVVGDGEGAGNGAGIAVEPFEAPRPEMVTRVVTASGGVSAAS
                     GAVAAIIGGLLGAAITLGLTRSTSRKVPARRREPA"
     gene            complement(729..1829)
                     /locus_tag="VF036_00500"
     CDS             complement(729..1829)
                     /locus_tag="VF036_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX6263737.1"
                     /translation="MENSDSAVAEFDGWLKELMVGKGSDLHVKVGSPPMIRLPGGLER
                     MPRDPITPVETAAIAESIIPADRKEAFDTTGEIDFAYSLRDVGRFRANVFKQRGSISM
                     VLRRLRFGGPTFEEMGLPDTVRRLADEHRGLILVTGPTGSGKTTTLASMIGYINETKP
                     VHIVTIEDPIEVLHRDAVASINQREIGNDTQDFLAALRAALRQDPDVILIGEMRDTET
                     VRAAIQAAETGHLVLSTLHTVDATETVNRVIDFFPPYQQKQVRLALAGTLRGIICQRL
                     VATVDGGRTPTLEILVNNGRIAERIVDPERTSEIEEVVADGAFYGMMTFDQSLLQLLQ
                     EGKISTEAAFEAVSSRHDFELAMQQVGLSLPV"
     gene            complement(1949..2623)
                     /locus_tag="VF036_00505"
     CDS             complement(1949..2623)
                     /locus_tag="VF036_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6263738.1"
                     /translation="MNEPAPIEIHKALADDTRYRLYRYLRLSGRPVPVRELATRLSLH
                     ANTIRPHLRRLADAGLVASETRRGPSAVGRPQTVYTAVATGDREGRDYRLLADILATL
                     TTGSRARAGAHTLAREWGDYLVTRHAPPPGARRPGPNLAGLREALAEAGFDPRFRRKG
                     SRTVEIALRGCPFRDLLEEHRELVCSVHRGLLEGMLEGSRPRLHLMAFEPLADRSSVC
                     RVVAGA"
BASE COUNT          444 a          952 c          975 g          448 t
ORIGIN      
        1 gtgctcctgt ggcgggacgg cccccacacg atgcggttgg agacctccct gccgatgacc
       61 gaggccctgc ggatcgccgc gtcgacggga acgccctgat ccgatccggt gtagcagagg
      121 catcggacgt caggagggat cgacatgaga cgagggacgc tgctctgcac gatcgtggtg
      181 ctcgcggccg tcgtggggat gcccggcgtg gcgtcggcgg gcggtggctg ccacgggggc
      241 gtcacgcaga ccgacgcaac cggccaggcg gacgcgaccg tgcagctggt cgacgcatgc
      301 ttctcggcga cggtcacgag cgtggatccg gggacgcccg tgaccttcgt gaacatggac
      361 gagttcgtcc ataacgtcgg tggcaaccag tggggtcact tcgacgacct ccacggggga
      421 gacaccttca gggtgtcctt cgaggaagcc ggcacgtacc cgttcgcgtg cagctatcac
      481 ccgggcatga ccggcgcgat cgtcgtcggc gacggcgagg gtgccggcaa cggcgcgggc
      541 atcgcggtcg agccgttcga ggcaccgcgt cccgagatgg tcacgcgtgt ggtcactgcg
      601 agcggaggtg tctcggcagc ctccggcgcg gtcgccgcga tcatcggcgg gctgcttgga
      661 gccgcgatca ccctggggtt gacccgttcg acttcgagga aggttccggc tcgacgtcgt
      721 gaaccggctt aaacgggcag gctcagcccc acttgctgca tcgcgagctc gaagtcgtgc
      781 cggctcgaga cggcctcgaa cgccgcctcc gtgctgatct tcccctcctg gaggagctgc
      841 agcagcgact ggtcgaacgt catcatcccg tagaaggcgc cgtcggcgac cacttcctcg
      901 atctcactcg tgcgctccgg atcgacgatc cgctcggcga tccgtccgtt gttcacgagg
      961 atctcgaggg tcggggtcct gccgccgtcg accgtggcga cgaggcgctg gcagatgatg
     1021 ccgcggagcg tcccggcgag cgccaaccgc acctgcttct gttggtaggg cgggaagaaa
     1081 tcgatcaccc ggttgacggt ctccgtcgcg tcgaccgtgt ggagcgtcga caggacgagg
     1141 tgtcccgtct cggccgcctg gatcgcggcc cgcacggtct cggtatcccg catctcgccg
     1201 atcaggatga cgtcgggatc ctgccgcagc gccgcacgca acgccgcgag gaagtcctgc
     1261 gtgtcattgc cgatctcacg ctggttgatc gaggccaccg cgtcgcgatg cagcacttcg
     1321 atcgggtcct cgatcgtgac gatgtgcacc ggcttggtct cgttgatgta gccgatcatc
     1381 gaagcgaggg tcgtggtctt gcctgatccc gtggggccgg tgacgaggat cagccctcgg
     1441 tgttcgtcgg ccagacgacg gaccgtgtcc ggcaacccca tctcttcgaa ggtgggtcca
     1501 ccgaaccgca gacggcggag caccatcgag atcgagcccc gctgcttgaa gacgttggcc
     1561 cggaaccggc cgacgtctcg cagggaatag gcgaagtcga tctcccccgt cgtgtcgaac
     1621 gcctccttcc ggtccgccgg gatgatgctc tcggcgatcg cggcggtctc cacaggcgtg
     1681 atcgggtcgc gaggcatcct ctccagccca ccgggcagcc tgatcatcgg cggcgagccg
     1741 accttcacgt gcaggtccga gcccttcccg accatgagct ccttgagcca gccgtcgaac
     1801 tccgcaacgg cggaatcact gttctccact gcgccacacc tcgtctcgag cgatggccga
     1861 gcacgcgcac ccatccgcgc tcggttcctc cctctcccat cggcccgtcg gaggaccaac
     1921 ttcagcgcga tcgagcgagt tgccgtgctc aggcgccggc gaccacgcgg cagaccgagc
     1981 tgcggtcggc caagggttcg aacgccatga ggtgaaggcg ggggcgagat ccctccaaca
     2041 tcccctccaa cagacctctg tgcaccgagc acacgagctc cctgtgctct tcgagcaggt
     2101 cgcggaacgg acagccgcgc agggcgatct ccacggtgcg ggaccctttg cgccggaagc
     2161 gagggtcgaa acccgcttcg gccaacgcct ccctcagccc cgccaggttc ggccccggac
     2221 ggcgcgcacc gggcggcggg gcgtgccggg tgacgagata gtcgccccac tctcgagcga
     2281 gggtatgggc acccgcccga gcgcgcgacc cggtcgtgag cgtcgccagg atgtccgcga
     2341 gcaatcggta gtcacggccc tcgcgatcgc ccgtggccac ggcggtgtag acggtctggg
     2401 ggcggccgac ggccgacggg cctcgtcgcg tctccgacgc gacgagcccc gcgtccgcga
     2461 gccgacgcag gtgtgggcgg atcgtgttgg cgtgcaggct cagccgggtg gcgagctcgc
     2521 gaacgggaac cgggcggccg gacaagcgca ggtaccgata gagccggtag cgcgtgtcgt
     2581 ccgcgagggc tttgtggatc tcgatcgggg ccggttcgtt catctccgca tctcgtacga
     2641 tgggctttgc acggttcgca ccgtgtcaac cagagcttag acctcagacc cgaacgagac
     2701 ccacgaacga agccaggcac ccactcgaga gaggagagcc cgtggccacg gaggcgcagg
     2761 tccgggaggc cctgaagggc gtccgggatc ccgagatcgg ccgcccgatc gacgagatc
//
LOCUS       DASUVE010000025         2765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_578203, whole genome shotgun sequence.
ACCESSION   DASUVE010000025 DASUVE010000000
VERSION     DASUVE010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578203"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..273
                     /locus_tag="VF036_00510"
     CDS             73..273
                     /locus_tag="VF036_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HEX6263739.1"
                     /translation="MPEQERKQVKKGGAGDESDADAGQVSATSKAAELKEEMDDILDE
                     IDSVLEENAEEFVKSYVQKGGE"
     gene            270..1064
                     /gene="prcB"
                     /locus_tag="VF036_00515"
     CDS             270..1064
                     /gene="prcB"
                     /locus_tag="VF036_00515"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEX6263740.1"
                     /translation="MTDRVELPLSGASFGADPSFVELLRRQHPEALPSSLQMTGPLEV
                     AQGTTVLGLRFADGIVMAGDRRATAGYTIADAKMKKVFAADDFSAIAIAGAAGQAVET
                     VRVFQLELEHYEKLTGDRLSLEGKANRLAQMIRANFPLAMQGLVVVPLFGGYDVARSE
                     GRLFYYDATGGRWEEEDYQATGSGAQPAKNSLKKRWRPGLSHEEALRVAAEALIDAAE
                     DDVATGGPDLARGIFPVVVTVTGLGATESTEEDVASAVRAVLEARA"
     gene            1065..1751
                     /gene="prcA"
                     /locus_tag="VF036_00520"
     CDS             1065..1751
                     /gene="prcA"
                     /locus_tag="VF036_00520"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HEX6263741.1"
                     /translation="MSFMPYVSPDQLMKDRSEYAHKGIARGRSVIGLEFADGLLFIAE
                     NPSATLHKISEIYDRIAFAGVGKYSEFEDLRIAGIRLADLRGYSYGREDVKARDLANA
                     YSQALSTIFTQQMKPYEVEILVGEVNGEIDGTSIYHVLFDGSVTDEHGFVAIGGHADD
                     LTEELKERYNAGWDLATAVKTAVQVLGAPDQRTIEHDHIESGVLERSQSRRRKFRRLT
                     DDEIAGFLAG"
     gene            1835..>2765
                     /locus_tag="VF036_00525"
     CDS             1835..>2765
                     /locus_tag="VF036_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018219798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HEX6263742.1"
                     /translation="MERRIFGLENEYGVTCTFRGQRRLSPDEVARYLFRRVVHWGRSS
                     NVFLENGARLYLDVGSHPEYATPECDEVEELVAHDKAGERILEALLGAAELRLHEEGI
                     SGQVYLFKNNTDSAGNSYGCHENYLVARHGEFARMADVLIPYFVTRQIWCGAGKVLHG
                     PRGAQYCISQRAEHIWEGVSSATTRSRPIINTRDEPHADAERFRRLHVIVGDSNMSEW
                     TSYLKVGITDLVLRMVEGNTVMRDLTLENPIRAIREISHDTTGTRKVKLANGRELSAI
                     EMQQEYFEKTSRFLERRGTDDASKKLLYEWGEAL"
BASE COUNT          503 a          882 c          972 g          408 t
ORIGIN      
        1 gctccaggga tagatcgcgc agctcgaagg gtctcgcggc ccggcggcta caatcgccac
       61 ggaggaggaa ccatgcctga acaagagcgc aagcaggtca agaagggcgg agccggcgac
      121 gagagcgacg ccgacgccgg ccaggtctcc gcgacctcga aggcggccga gctcaaggag
      181 gagatggacg acatcctcga cgagatcgat tcggtcctcg aggagaatgc agaggagttc
      241 gtgaagtcct acgtgcagaa gggcggcgag tgaccgatcg ggtcgagctc ccgctgtcgg
      301 gagcctcgtt cggcgccgac cccagcttcg tcgagctcct tcgtcgtcag catcccgagg
      361 cgctgccgtc gtcgctccag atgaccgggc ccctggaggt cgcgcagggc acgaccgtcc
      421 tcggattgcg cttcgcggac ggcatcgtca tggccggtga ccgccgagcc acggccggct
      481 acacgatcgc ggacgcgaag atgaagaagg tgttcgccgc agacgacttc tccgcgatcg
      541 cgatcgcggg tgcggcgggc caggccgtag agaccgtccg ggtgttccag ctcgagctcg
      601 agcactacga gaagctcacc ggggatcgac tgtccctgga aggcaaggcg aaccggctcg
      661 cgcagatgat ccgtgccaac ttcccgctcg cgatgcaggg gctcgtggtg gtcccgctct
      721 tcggcgggta cgacgtcgcg aggagcgaag gccggctctt ctactacgac gccacggggg
      781 ggcgctggga ggaagaggac tatcaggcga ccggctcggg agcgcagccc gcgaagaact
      841 ccctgaagaa gcggtggagg cccgggcttt ctcacgagga agcgctccgc gttgccgccg
      901 aggcgctgat cgacgcggcc gaggacgacg tcgcgacggg cggccccgac ctcgcccgag
      961 gcatcttccc ggtggtcgtc acggtgacgg ggctgggtgc gacggagtcc accgaagagg
     1021 acgtcgcgag cgccgtccgg gccgtgctgg aagcgagggc ctagatgtcg ttcatgccct
     1081 acgtctcgcc cgatcagctg atgaaggatc ggtcggagta cgcccacaag ggcatcgcgc
     1141 gcgggcgctc cgtgatcggc ctcgagttcg ccgacggcct gttgttcatc gccgagaacc
     1201 cgagcgccac cctgcacaag atcagcgaga tctacgatcg gatcgcgttc gcgggcgtcg
     1261 ggaagtacag cgagttcgag gacctgcgga tcgccggcat ccggctggcg gacctccgcg
     1321 gctactccta cggtcgcgag gatgtgaagg cgcgggacct cgcgaacgcg tactcgcagg
     1381 cgctctcgac gatcttcacg caacagatga agccgtacga ggtggagatc ctcgtgggcg
     1441 aggtgaacgg agagatcgac ggcacgtcga tctatcacgt cctgttcgac gggagcgtga
     1501 ccgacgagca cgggttcgtc gcgatcgggg gccacgcgga cgacctcacc gaggagctca
     1561 aggagcggta caacgccgga tgggacctgg ccaccgcggt gaagaccgcc gtgcaggtgt
     1621 tgggcgctcc tgaccagcgc acgatcgagc acgatcacat cgaatcgggc gtgctcgagc
     1681 gcagccagag ccgacgtcgc aagttccgca ggctgacgga cgacgagatc gcaggcttcc
     1741 tcgccggctg agccgtgggc tccccgggcc tcgccgaccg cgacccgcac ccggatcttc
     1801 tctgggagat cacgggctgg cctagactcg gtccatggag cggaggatct tcggcctcga
     1861 gaacgagtac ggcgtgacgt gcacgttccg cgggcagcgc cggctctcac cggatgaggt
     1921 ggcgcgctac ctcttccgcc gggtggtgca ctggggccgc tcgtcgaacg tgttcctcga
     1981 gaacggggcc cgcctgtacc tcgatgtcgg gtcgcatccg gagtacgcga cgcccgagtg
     2041 cgacgaggtc gaggagctcg tggcccacga caaggcgggg gagcggatcc tcgaggcgct
     2101 cctgggcgcc gccgagctcc ggctccacga ggagggcatc tccggtcagg tctatctctt
     2161 caagaacaac accgactcgg cgggcaactc ctacggttgc cacgagaact acctcgttgc
     2221 ccggcacggg gagttcgctc ggatggccga tgtcctgatc ccgtacttcg tgacccgcca
     2281 gatctggtgt ggcgcaggga aagtgctaca cgggccgagg ggcgcccagt actgcatctc
     2341 ccagcgggcg gaacacatct gggaaggcgt ctcctcggcg acgacccgat cgaggccgat
     2401 catcaacacg cgcgacgagc cgcacgcgga cgccgagcgg ttccgccgtc tccacgtgat
     2461 cgtcggtgat tcgaacatga gcgaatggac cagctatctc aaggtcggca tcacggacct
     2521 cgtcctcagg atggtcgagg gcaacacggt gatgcgggat ctcaccctcg agaacccgat
     2581 ccgcgcgatc cgggagatca gccacgacac caccggcacg cggaaggtga agctggccaa
     2641 cggcagggag ctgtccgcga tcgagatgca gcaggagtac ttcgagaaga cctcgcgttt
     2701 cctcgagcgg cggggaaccg acgacgcctc gaagaagctc ctctacgagt ggggcgaggc
     2761 tctcg
//
LOCUS       DASUVE010000026         5042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_201667, whole genome shotgun sequence.
ACCESSION   DASUVE010000026 DASUVE010000000
VERSION     DASUVE010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201667"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VF036_00530"
     CDS             complement(<1..565)
                     /locus_tag="VF036_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263743.1"
                     /translation="MRRRSPHRSKLTVVLAVALGLLTPMAANMVGAQAGASTAVPSGT
                     SAAAVVASPPPRGLALPASGVRPDPREVARAATRVARWTNVLVEVPRTLPAFAWPRPG
                     ATQVGVVPSGSKYFGIGIVAWVEEVSSDGRWGRVELPYTWPRRDGWIRLSGLDRDATR
                     VRVEVDLSLHQVTVRRFGKVLFRAPGAT"
     gene            729..1745
                     /locus_tag="VF036_00535"
     CDS             729..1745
                     /locus_tag="VF036_00535"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX6263744.1"
                     /translation="MDAMRETARELVAPGKGILAADESSGTIEKRFDQIGVGSTEDTR
                     RAYRQLLFTTPGLEEHISGVILFDETIRQSTDDGIRFTEVLEKTGVIPGIKVDTGAKP
                     LALFEGETVTEGLDGLRERLAEYRELGARFAKWRATYFIGDGRPTDLAVLANGHAMAR
                     YAALCQEAEIVPIVEPETLMDGEHDLAACEDATGRALDGLYAQLRAQRVDLAGTLLKV
                     NMVVPGKGASMQADDTTIADATLRTLRAHVPDEVPGIVFLSGGMTDEQATSRLNEINR
                     LDEAPWEISFSYGRALQQRPLTLWGGDAANGEAAQAALAHRARMNGLARDGGWSQDLE
                     GSAA"
     gene            1810..2796
                     /locus_tag="VF036_00540"
     CDS             1810..2796
                     /locus_tag="VF036_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6263745.1"
                     /translation="MKILTIGGTRFFGRAFVEEAVDRGHDVTVFHRGGSEPSDLPSVE
                     HIHGDRNLGVETLRGRSWDAVLDTCAFVPREVRELADTIGDGIGHYTLVSSLSVHPDD
                     LPVGATEVTPVHPPPYPDTEDVTEETYGPLKVACEVEAAARFGDRVLVIRPGYIVGPH
                     DPTDRFTWWVRRAAAGGEMLAAGPPDARIQGIDARDLGAFVLDRVEAQDADTYGVIGP
                     AEPATTADVVEGARSAAGADTSVVWVDPAFVRALGEIHETWFPMWHPHLPGYHAYDAA
                     KATAAGLRPRPFERTIADTLAWDRDRGAPPLAAGVPPDKERELLDAWRARAS"
     gene            complement(2801..4768)
                     /locus_tag="VF036_00545"
     CDS             complement(2801..4768)
                     /locus_tag="VF036_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX6263746.1"
                     /translation="MPATARAGTVRAVPDERRSIEMDDGARIAISLHLPDEHPPPWPV
                     ILEAQPYRKDDFSDSTATYRRLADEGGLAVCRADVRGTGSSEGVAEDEYSDREWLDHE
                     TTIAWLAEQEWSNGNVGMWGTSYSGFNALQMAVRRPPALKAIIPIFATDRRYTDDVHF
                     GGGARRGIDFLDYPTYMVAFNALPPVPFAYGAGWREEWRRRIEANEPWVLHWLEHQNE
                     DAYWRRGSSIDAYDRIEAATLVIAGHADGYRNMAFRAFERLRGPKQILFGPWAHWSPR
                     VSMPGPRIDHVPVMIAWWDRWLRDNPSIDTGPPIAIYVRRWTEPEPDTDRYAGEWRAE
                     PTWPPARLAEDRRLLSGADESSTGDELRIRGDVGVTGSIWCAGLPPFGLPWDQRPDEA
                     FSLVYDWPVSAAIEIMGHPRVELTVSSSTPVAYVSAKLCDVAPDGTSALVTRGILNLT
                     HRESHERPEALPTGEPVRATIELDATAYEFAPGHRIRLDLAPSDFPSSWPPPHAGTIV
                     VDRAASALVLPTLEGPPVAPAPEFAPGAPEGTAPDRVRWEIHQDVLARERAVEIDHGG
                     VRGRVGITETSDRYHGEIRVRWDDPGHCTATAGAELELAWPEATVRAESRTILRSDPE
                     TWNLAIELQVYEADELIAERRWERTTPRDLQ"
     gene            4848..>5042
                     /locus_tag="VF036_00550"
     CDS             4848..>5042
                     /locus_tag="VF036_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263747.1"
                     /translation="MRARAWKSAAIVAATPLMLLVAAPPASAAPFFTSVPLMITLADA
                     STSKATPIISVGDEVGDFFFE"
BASE COUNT          761 a         1730 c         1821 g          730 t
ORIGIN      
        1 ccgtcgcgcc gggggcgcgg aacaggacct tgccgaacct ccgcaccgtg acctgatgca
       61 gggacagatc gacctcgacg cgcacgcggg tcgcgtctcg gtccaggccg ctgaggcgga
      121 tccaaccgtc gcgccgcggc cacgtgtagg gaagctcgac gcgtccccac ctgccatccg
      181 atgagacctc ttcgacccag gccacgatcc cgatcccgaa gtacttggag ccggagggga
      241 cgacgcccac ctgcgtagcg ccgggacggg gccacgcgaa cgcaggaagg gtgcgcggca
      301 cctcgacgag gacgttggtc caccgagcga cgcgggttgc cgctcgcgcg acctcccggg
      361 gatccggccg aacgcccgat gccgggagcg cgagcccccg agggggcggg ctcgccacca
      421 cggccgcggc cgaggttccg gagggcaccg ccgtgctcgc ccctgcctgc gccccgacca
      481 tgttcgccgc catcggggtt agcaagccca gcgcgacggc cagcacgacc gtgagcttcg
      541 aacggtgggg ggagcgtcgc cgcatgcctc cctatcggcg ggtgcggcga actacctaag
      601 tcgaggtagt cgattgcccg cagcgtgctg ctggccgtag cctggagcga cggcgtcgac
      661 cgagagacca agctcgccga cgagtgccga agagtgccaa gagtgccgaa gacagaagga
      721 gcggacggat ggatgcgatg agggaaacgg cacgcgagct ggtggccccc ggcaagggga
      781 tcctcgcggc ggacgagtcc agcggaacga tcgagaagcg cttcgaccag atcggcgtcg
      841 gatcgacgga agacacgcgg cgcgcctacc gacagctcct cttcaccacc cccggtctcg
      901 aggagcacat ctccggcgtg atcctcttcg acgagacgat ccgtcagtcg accgacgacg
      961 ggatccgatt caccgaggtg ctcgagaaga cgggagtgat cccggggatc aaggtggaca
     1021 ccggtgccaa gccgctcgcg ctgttcgagg gcgagaccgt gacggaggga ctcgacgggc
     1081 tccgggagcg cctcgcggag taccgggagc tcggcgcgcg cttcgcgaag tggcgcgcca
     1141 cgtacttcat cggcgacggt cgacccaccg atctcgcggt gctcgcgaac gggcacgcga
     1201 tggcgcgcta cgccgcgctc tgccaggaag ccgagatcgt cccgatcgtc gagccggaga
     1261 ccctgatgga cggagagcac gacctcgcgg cgtgtgaaga cgccaccggc cgtgcgttgg
     1321 atgggctcta cgcgcagctc cgggcccagc gggtcgatct cgccggcacg ctcctgaagg
     1381 tgaacatggt ggttcctggg aagggcgcat cgatgcaggc ggacgacacc acgatcgcgg
     1441 acgccacgct ccgaacgctc cgagcccatg tccccgacga ggtgcccggg atcgtgttcc
     1501 tgtccggtgg gatgaccgac gagcaagcga cgagccgact caacgagatc aaccgtctcg
     1561 acgaggcgcc gtgggagatc tccttctcgt acggccgcgc gctccaacag cgaccgctca
     1621 ccctgtgggg aggcgatgcc gccaatggtg aggcggcgca agcggcgctg gcgcatcgcg
     1681 ctcggatgaa cggcctcgcg cgcgatggcg gatggtcgca ggacctggag gggtccgcgg
     1741 cctgaccgga cggggcgcac gtcccacctt gtggcgcgga cccgtctccc gggacggtag
     1801 ggtccgtcgg tgaagatcct gacgatcggc ggtacgcggt tcttcggccg cgccttcgtg
     1861 gaagaggcgg tggatcgtgg tcacgacgtg accgtcttcc atcgaggggg atcggagccc
     1921 tcggatctcc cgtccgtcga acacatccac ggcgaccgga acctgggtgt ggagacgctt
     1981 cgggggcgtt cctgggacgc ggtcctggac acgtgtgcct tcgtcccccg cgaggtccgc
     2041 gagctcgcgg acacgatcgg cgacgggatc ggccactaca cgctggtgtc ctcgctgtcg
     2101 gttcatccgg acgacctgcc ggtcggtgcg acggaggtca ccccggtcca cccaccgccg
     2161 tacccggata cggaggacgt gaccgaggag acctacggac ctttgaaggt ggcgtgcgag
     2221 gtggaggccg cggctcggtt cggcgaccgg gtgctcgtga tccgtcccgg gtacatcgtc
     2281 gggccgcacg accccaccga tcgcttcacc tggtgggtgc ggcgggcagc cgcgggcggg
     2341 gagatgctcg ccgcaggccc gccggacgca cgcatccagg ggatcgacgc gcgcgacctc
     2401 ggcgcgttcg tcctcgatcg agtggaggca caggatgccg acacgtacgg cgtgatcggg
     2461 ccggccgagc cggcgacgac ggccgacgtg gtggaaggag cgcggagcgc ggcgggcgcc
     2521 gacacctcgg tcgtgtgggt cgaccccgcg ttcgtgcgag cgctcgggga gatccacgag
     2581 acctggttcc ctatgtggca tccccatctg cccgggtacc acgcctacga cgccgcgaag
     2641 gccacggcgg cggggctgcg tccccgcccg ttcgagcgca cgatcgcgga caccctcgcc
     2701 tgggatcgcg atcggggtgc accgccgctc gcggcgggcg taccgccgga caaggaacgg
     2761 gagctgttgg acgcctggcg ggctcgcgcg agctgagccc ctactgcagg tcgcgcggcg
     2821 tcgtgcgctc ccatcgccgc tctgcgatca gctcgtcggc ttcgtagacc tggagttcga
     2881 tcgcgaggtt ccacgtctcc ggatcggacc ggaggatggt tcgagactcg gcgcggaccg
     2941 tcgcttcggg ccaggccagc tcgagctccg cgccggccgt cgcggtgcag tgcccggggt
     3001 cgtcccatcg gacccggatc tcgccgtggt accggtccga ggtctcggtg atgccgaccc
     3061 gaccgcggac gccgccgtgg tcgatctcga cggctcgttc gcgggcgagg acgtcctggt
     3121 ggatctccca ccggacgcga tcgggcgccg ttccctccgg ggctccgggg gcgaactccg
     3181 gtgcgggagc gacgggcggt ccctccagcg tcggcagcac gagcgccgac gcggcacggt
     3241 cgacgacgat cgtcccggca tgaggcggcg gccacgacga agggaagtcg ctcggggcca
     3301 ggtcgagtcg gatccggtgc ccgggtgcga actcgtaggc cgtggcgtcc agctcgatcg
     3361 tcgcccggac cggctcaccg gtcggcagcg cctcggggcg ttcgtgcgac tcacggtggg
     3421 tgaggttcag gatcccgcgg gtcacgagcg ccgacgtacc gtcgggcgcc acgtcgcaga
     3481 gcttggcgga cacgtacgcg accggtgtcg acgacgacac ggtcagctcg acgcgcggat
     3541 gccccatgat ctcgatcgcg gcggagaccg gccagtcgta gacgagggag aacgcctcat
     3601 ccgggcgctg gtcccagggg agtccgaacg gaggcaaccc cgcgcaccag atcgagccgg
     3661 tcacaccgac gtccccgcgg atccgcagct cgtcgccggt cgatgactcg tccgcgccgg
     3721 acagcagacg ccggtcctcg gcgagccgag ccggcggcca tgtcggctcg gctcgccact
     3781 cgccggcgta cctgtccgtg tccggctcgg gctcggtcca ccgtcggacg tagatcgcga
     3841 tcgggggacc cgtgtcgatc gaggggttgt cgcggagcca tcggtcccac caggcgatca
     3901 tcaccggcac gtggtcgatg cgcggccccg gcatcgatac ccgtgggctc cagtgcgccc
     3961 acggtccgaa caggatctgc ttgggaccgc ggagtcgttc gaaggcacgg aacgccatgt
     4021 tccgatagcc gtcggcgtga ccggcgatca cgagtgtggc cgcctcgatg cgatcgtagg
     4081 cgtcgatgct cgagccgcga cgccagtacg cgtcctcgtt ctggtgttcg agccaatgca
     4141 gcacccacgg ctcgttggcc tcgatccgtc gacgccactc ctcgcgccat cccgcgccgt
     4201 aggcgaacgg aaccggcggc agcgcgttga acgcgaccat gtacgtgggg tagtcgagga
     4261 agtcgatgcc gcgccgggcg ccgccgccga agtggacgtc gtcggtgtac cgccggtcgg
     4321 tggcgaagat cgggatgatc gccttcagcg ccgggggccg tcgcaccgcc atctggagcg
     4381 cgttgaagcc cgagtacgag gtgccccaca tcccgacgtt gccgttcgac cactcctgct
     4441 cggcgagcca cgcgatcgtc gtctcgtgat cgagccactc gcggtcgctg tactcgtcct
     4501 cggcgacgcc ctccgacgat cccgtgccgc ggacgtcggc tcggcacacg gcgaggccgc
     4561 cctcgtcggc caggcgccgg taggtcgccg tggagtccga gaagtcgtcc ttccggtacg
     4621 gctgcgcctc gaggatcacg ggccacggag gtggatgctc gtccgggagg tgcagtgaga
     4681 tcgcgatcct tgcgccgtcg tccatctcga tcgatcggcg ttcgtccggc acggcccgca
     4741 cggtacccgc tcgtgcggtc gccggcatga ccggcgacca gcggatttga cgacccgctc
     4801 gcctcggcgg gagggtggag acatcccccc gacagaggag gttgtgcatg cgagcacgcg
     4861 cctggaagag cgccgcgatc gtcgcggcga cgcctctcat gcttctggtg gccgcgccgc
     4921 ccgcgtcggc cgcaccgttc ttcacgtcgg ttcccttgat gatcacgctc gccgacgcga
     4981 gcacttcgaa ggcgaccccg atcatcagcg tcggcgacga ggtgggtgac ttcttcttcg
     5041 aa
//
LOCUS       DASUVE010000027         2985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_579529, whole genome shotgun sequence.
ACCESSION   DASUVE010000027 DASUVE010000000
VERSION     DASUVE010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579529"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VF036_00555"
     CDS             <1..757
                     /locus_tag="VF036_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX6263748.1"
                     /translation="GFLPRGSERALLDAKGLALVAGFVPAVLHEPSSLERELARVDAA
                     AQTIASLGGGVLVLAASSGGHGYEGAERLDAAGWAALGRGIDRVVAATGERGVRVAVH
                     PHHGTVVQGPEDVGRLLEISDVGLCLDTGHVMVGAGDPLEVARGAADRVIHVHLKDVS
                     AGVAGRVAVGDLGYRDAVREGLYRPLGDGDLDVAGVVRALEESGYGGWYVLEHDEVLD
                     HEPEEGSGPIGNARRSLSLLGRVWEEVGAPTKG"
     gene            811..1806
                     /locus_tag="VF036_00560"
     CDS             811..1806
                     /locus_tag="VF036_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX6263749.1"
                     /translation="MMKRWAALALVFALGAAACTGNGDGDGGGDGDGNGGVTQRDFRF
                     VVVTHGQASDPFWSVAANGVDAAAEDMGVTVEYQAPGTFDMVEMSQLIDAAVASEPDG
                     LVVSLPDADALGPSIEAAVAADIPVITMNSGSDTFADLGVLLHVGQTEFEAGQIAGER
                     FAEEGVQNPICLNQEVGNAALDLRCEGFFDGLGVEGEVIAVDLDDPTSTTETVTGALQ
                     NNPDIDGILTLGPTGAAPTIAALQEAGTLGEIAFATFDLSPEVLEAIDAGDMLFAIDQ
                     AQYLQGYLPIVFLTKYLETGALPLGSKDRVLLTGPQIVTADTASQVVEASADGLR"
     gene            1893..2960
                     /locus_tag="VF036_00565"
     CDS             1893..2960
                     /locus_tag="VF036_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6263750.1"
                     /translation="MATEVEAPPKAADERVRATGTASRHLARPELGALVGVVLVYVFF
                     AIVAADNNIVSMTTLASILNRAAPLGILAIAVALLMIAGEFDLSIGSLLGFSGMAIMI
                     FVSPEDAGGFGLPLWPSVGLALVLTALTGVINGLLVNVTKLPSFIITLGTLFIFRGLT
                     IAVTRSLTSRTQLSGIDEIPGFSLANTLFGTRVEVAGANFNITIVWWIGLVVLATWVL
                     LRTRTGNWIFGTGGAVDAARNVGVPVSRVKLALFVTTAMAAFLVAMLQVVQVNSADAL
                     RGTGLEFEAIIAAVVGGCLLTGGYGSVVGAALGALLFAMVQQGIVITGVDGDWYQVVL
                     GSVLVVAVIFNNFIRRRAATR"
BASE COUNT          425 a          937 c         1138 g          485 t
ORIGIN      
        1 ggggttcctg ccccgcggct cggagcgagc gctcctcgac gcgaagggcc tcgcgctcgt
       61 ggcggggttc gtcccggcgg tcctgcacga gccgtcgtcc ctggagcgcg agctcgcgag
      121 ggtcgatgcc gcggcgcaga cgatcgcgtc gctcggcggc ggggtgctcg tcctcgcggc
      181 atcgtccggt gggcacgggt acgagggagc cgagcggctg gacgccgccg gctgggccgc
      241 gctcgggcga gggatcgacc gcgtcgtggc tgcgaccggg gaacgagggg ttcgtgtggc
      301 cgtgcacccg catcacggaa ccgtggtcca gggaccggag gacgtgggac ggctgctcga
      361 gatctccgac gtcggtttgt gcctcgacac cggtcacgtc atggtggggg cgggagaccc
      421 gctggaagtc gctcggggag ccgccgaccg cgtgatccat gtccacctga aggacgtgtc
      481 ggcgggcgtg gccgggcggg tcgccgtagg cgatctcgga taccgggacg cggtacggga
      541 gggtctgtat cggccgctgg gcgatggcga cctcgacgtc gcgggcgtcg ttcgcgcgct
      601 cgaggagtcc ggatacggcg gttggtacgt actcgaacat gacgaggtgc tcgatcacga
      661 gcccgaggaa ggatcggggc ccatcgggaa cgcgaggcgg agcctctcgt tgctggggcg
      721 ggtctgggag gaggtggggg cgccgacgaa gggctagtcc gtggaggcgc gagcgtcgcg
      781 cccagcgaca gatacgaggg ggttggttcg atgatgaaga gatgggcggc gctggcgctc
      841 gtattcgcgc tcggcgcggc agcatgtacc gggaacggtg acggagatgg cggcggtgac
      901 ggcgacggga acggcggcgt cacacagcgt gacttccggt tcgtggtcgt cacccacggt
      961 caggcgtcgg acccgttctg gtcggtcgcg gccaacgggg tcgacgcggc ggccgaggac
     1021 atgggcgtca cggtcgagta ccaggcgccc ggcacgttcg acatggtgga gatgtcgcag
     1081 ctgatcgacg cggcggtcgc ctccgagccg gacgggctcg tcgtgagtct cccggacgcc
     1141 gatgcactcg gtccgtcgat cgaggctgcg gtggcggcgg acatcccggt catcacgatg
     1201 aactcgggat cggacacctt cgccgacctc ggcgtgctcc tgcacgtcgg gcagacggag
     1261 ttcgaggccg gccagatcgc gggggagcgg ttcgccgagg agggcgtcca gaacccgatc
     1321 tgcctgaacc aggaggtcgg caacgcggcc ctcgacctgc gctgtgaggg gttcttcgat
     1381 gggctcgggg tggagggcga ggtcatcgcc gtggacctcg acgacccgac gagcacgacc
     1441 gagacggtga ccggggcctt gcagaacaac ccggacatcg acgggatcct gacgctcggc
     1501 ccgaccgggg ccgcgccgac gatcgcggcg ctccaggagg ccggcacgct cggggagatc
     1561 gcgttcgcga ccttcgacct ctcgccggag gtgctcgagg cgatcgacgc gggcgacatg
     1621 ctgttcgcga tcgaccaggc gcagtacctg caggggtacc tgccgatcgt gttcctcacg
     1681 aagtacctgg agacgggagc cctgccgctg ggaagcaagg accgggtgct cctgaccggt
     1741 ccgcagatcg tgacagcgga caccgcttca caggtggtgg aagcctccgc agacggactg
     1801 cggtagcggg atgagcggag ccctcgcgcg cgaggtcgcg cgcgagggcc ctccgttcta
     1861 ggctcacccc gaacacggcg aggaggggag gcatggccac cgaagtcgaa gcgccaccga
     1921 aggcggccga cgagagggtc cgtgcgaccg gcacggcgtc gcgccacctc gcgcgtcccg
     1981 agctcggcgc gctcgtcggg gtcgtgctcg tctacgtctt cttcgcgatc gtcgccgccg
     2041 acaacaacat cgtgagcatg acgacgctcg cgtcgatcct gaaccgggcc gctcctctcg
     2101 ggatcctcgc gatcgccgtc gcgctcctga tgatcgccgg cgagttcgac ctttcgatcg
     2161 ggtccctgct gggcttctcg gggatggcga tcatgatctt cgtctcgccg gaggacgccg
     2221 ggggattcgg attgccgctg tggccgtccg tcgggttggc gctcgtcctg accgcgctga
     2281 ccggagtgat caacggcctc ttggtgaacg tcacgaagct cccgtcgttc atcatcacgc
     2341 tgggcacgct tttcatcttc cgcgggctca cgatcgccgt gacgcgctcg ctgacctctc
     2401 ggacgcagct gagcggcatc gatgagatcc ccgggttctc cctcgcgaac acgctgttcg
     2461 ggacgagggt ggaagttgct ggggcgaact tcaacatcac gatcgtctgg tggatcggcc
     2521 tcgtcgtttt ggcgacttgg gtcctgctcc ggacgcgaac ggggaactgg atcttcggga
     2581 ccggcggcgc cgtggacgcg gcgcggaacg tcggtgtccc ggtcagccgc gtgaagctgg
     2641 cgctcttcgt gacgacggcc atggccgcgt tcctcgtggc gatgctccag gtcgtccagg
     2701 tgaacagcgc ggatgcgctc cggggcaccg gcttggagtt cgaggcgatc atcgcggcgg
     2761 tcgtcggcgg gtgcctcctc accgggggct acggctccgt cgtcggcgcc gcgctcggtg
     2821 ccttgctctt cgcgatggtg cagcagggga tcgtgatcac cggcgtggac ggcgactggt
     2881 accaggtggt gctcggcagc gtcctcgtgg tcgcggtgat cttcaacaac ttcatcagga
     2941 ggagggcggc gacccgatga gcgcgcagac cgacgacgcg cttct
//
LOCUS       DASUVE010000028         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1145085, whole genome shotgun sequence.
ACCESSION   DASUVE010000028 DASUVE010000000
VERSION     DASUVE010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145085"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="VF036_00570"
     CDS             <1..1298
                     /locus_tag="VF036_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263751.1"
                     /translation="AGGASVWFQWTAAREGSLTVWVNVPTFDTVMGVYTGAAVGALTE
                     VASNDDYGASTASRVTFPVDVGTTYRIAVDGVGGTEGPFRLRWRQGPVNDFFADAEVL
                     TGPSGSVEGSSFGATAEPGEASELSAVSTWFRWTAPEDGSFGFHVRSAEGLTVYTGSS
                     PGTLVALGPTGTELVFTAVAGTEYSVRVAGFTWNDGFPFTLFWGESPTNDAFANAIEI
                     DGSTGKTAGSTAFAGEETGEPPRGTATVWYTWTAPTTGHFRIDAWMVDDTAWGWINTT
                     LAVYRGATVDALTLVARNTGWWQSPLPEAGSAVSFRAVAGTAYSIAVDSGGSFSWGPF
                     ELRWYPGAIIFGKAGSDQIKGTAGRDYIDGRGGNDVLRGLGGSDYVVGGSGRDRLFGE
                     GGADLLDSRDFVRGNDEIDGGSGTDRAIRDRQDTVRTVP"
     gene            complement(1363..>2074)
                     /locus_tag="VF036_00575"
     CDS             complement(1363..>2074)
                     /locus_tag="VF036_00575"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX6263752.1"
                     /translation="AYVSGLSAAFKDIADRIYERMPLFLLYIIGVTFIVLAMAFRSIV
                     ISLTAAITTILSAFVAFGVLTLVVQEGYLLGLTGLDRTGPIETFVPPIAFAILFGLSM
                     DYMVFLMSRIREEHVHGLNTREAVEHGIAAIGRVVVAAALIMGTVFAAFILTADRISK
                     EFGLLLAVAILTDALIVRMTLVPAFLTLMGEKSWYIPRWLDRLLPDITIEPPHDRDAY
                     YPVAEPADEPAREPAGRT"
BASE COUNT          354 a          664 c          738 g          318 t
ORIGIN      
        1 aggcaggagg cgcatccgtc tggttccagt ggacggccgc gagggaaggc tcgctgaccg
       61 tgtgggtgaa cgtgccgacg ttcgacacgg tcatgggcgt ctacacggga gcagccgtcg
      121 gtgcgctgac agaggtcgcc agcaacgacg actacggggc gagcacggcg agccgtgtca
      181 cctttccggt cgacgtcggt acgacatacc ggatcgctgt agacggtgtc ggcgggacgg
      241 aagggccgtt ccggcttcgc tggaggcagg gcccggtgaa cgacttcttc gctgacgccg
      301 aggtgctcac gggaccttcc ggctcagtcg aggggtcgag cttcggcgcg accgccgagc
      361 cgggggaggc gagcgagttg tccgctgtct ccacctggtt tcgctggacc gcccccgaag
      421 acggcagctt cggcttccac gttcgctctg ctgaagggct gaccgtctac acgggctcga
      481 gccccgggac gctagtggcc ttgggcccga ccgggacgga actcgttttc actgctgtgg
      541 cgggaaccga gtacagcgtc cgggtggccg ggttcacctg gaacgacggg ttcccgttca
      601 cgctcttctg gggcgagtcg cccacgaacg acgcgttcgc gaacgcgatc gagatcgacg
      661 ggtcgacggg gaagacggcc ggttcgaccg ccttcgccgg cgaggaaacg ggggaaccgc
      721 caagaggcac agccaccgtt tggtacacgt ggacggcgcc cacgaccggg catttccgaa
      781 tcgatgcgtg gatggtcgac gacaccgcct ggggctggat caacacgacc ctcgccgtct
      841 acagaggcgc gactgtggac gccctcacgc tcgtggcaag aaacaccggc tggtggcaat
      901 ccccgctccc cgaggccggg agcgccgtca gcttccgcgc ggtcgcgggc accgcgtact
      961 ccattgccgt cgacagtgga ggctcgttct cgtgggggcc gttcgagctc cgttggtatc
     1021 cgggcgcgat catcttcggc aaggccggaa gcgaccagat caaaggaacc gcgggccgcg
     1081 actacatcga cggccgtggc ggcaacgacg tcctccgcgg gctcggtggc agtgactatg
     1141 tcgtcggcgg gtccggacgc gatcgcctct tcggggaagg tggtgccgac ctcctcgaca
     1201 gccgcgactt cgtccggggc aacgacgaga tcgacggggg ctccggcacg gatcgcgcga
     1261 tccgtgatcg ccaggacacg gtgcgcaccg tgccgtagct ccggcggatg atggacgagg
     1321 ttgggagcgg cgaccccacg gtcgccgctc ccattcggtc ttctaggtcc gtccggccgg
     1381 ctcccgggcc ggctcgtcgg ccggctcggc caccgggtag tacgcgtccc ggtcgtgcgg
     1441 gggctcgatc gtgatatccg gcagcaggcg gtcgagccag cgcgggatgt accacgactt
     1501 ctcgcccatg agcgttagga acgccgggac gagcgtcatc cgaacgatga gcgcgtccgt
     1561 gaggatcgcg actgccagca agagcccgaa ctccttggag atgcgatccg cggtgaggat
     1621 gaaggccgcg aagacggtgc ccatgatcag cgccgccgcg acgaccactc gaccgatggc
     1681 cgcaatcccg tgctcgacgg cctcccgcgt gttgaggccg tggacgtgct cctcgcggat
     1741 gcggctcatg aggaacacca tgtagtccat cgagagcccg aagaggatcg cgaacgcgat
     1801 cggcggcacg aacgtctcga tcgggcccgt ccggtcgagg ccggtgagcc cgagtaggta
     1861 gccctcctgc accaccagcg tgagtacgcc gaacgctacg aacgccgaga ggatcgtcgt
     1921 gatcgcggcc gtcagcgaga tgacgatcga gcggaacgcc atcgccagga cgatgaacgt
     1981 cacaccgatg atgtagagga ggaagagcgg catccgctcg tagatccgat cggcgatgtc
     2041 cttgaaggcc gccgacagac ccgacacgta ggcg
//
LOCUS       DASUVE010000029         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1333796, whole genome shotgun sequence.
ACCESSION   DASUVE010000029 DASUVE010000000
VERSION     DASUVE010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333796"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="VF036_00580"
     CDS             <1..1249
                     /locus_tag="VF036_00580"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6263753.1"
                     /translation="KAALVGLVLFDPTDQAVPFFAGALLIMSVNRFFLSTAAAVVPRL
                     VPHEDLLVANSLATVGGTVALLAGVFVGGRVIDVVDVTVPVVIAAGFGWLAASWIASR
                     IVSSLKPHTKPEDPELLRHQLRRVGVEFSDGARRLLRSPRAIGPIASITLDQIGQGIV
                     LTLSLVVFRHEFGEGVASFSNLIGAGGVGVLVGILTVGKLEERFSKPRIVAGAFVVGG
                     VVLFAVSFLLRDWTILLASFATGLTFAWKKISVDTMVQEAVPDGYRGRVFSVYDVVYN
                     LARVIAAALAIPMIPRLGVEGSLAIVGIVFLLWPPVIGRWYGREPELEIRFVAGGRAD
                     ETPRAISWGGAEEPVELVRTWREERDGQRRLAFRLALPDGTQLDVSRPEPDGPWRLDR
                     EDDRLPSDSGGSRIVSGDRTEPP"
     gene            1246..1860
                     /locus_tag="VF036_00585"
     CDS             1246..1860
                     /locus_tag="VF036_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263754.1"
                     /translation="MNERSGRNALIVGAIVLALLSIGAGTLIALSDDDERASTPTTTS
                     SLTPSAEPSDEPTVAPDPTMPASPEPPGSESPILEDGHHFVYIAGASRLEAGTSDVEF
                     DLAYFYVGGSAEREAAERDDEVLDGYYIVNENDRLRTLPLAEDVEVAYIPVDACCDLQ
                     PGNVDAWLEAVLQTNQTDYGGADVPWWFTVESGEITRIEQQYLP"
     gene            complement(1829..>3060)
                     /locus_tag="VF036_00590"
     CDS             complement(1829..>3060)
                     /locus_tag="VF036_00590"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6263755.1"
                     /translation="ASMHVSRNFRLFFVGQLISVTGTWVNWTAGAVLVLKLTDSGVAL
                     GIEAALLFLPVLLFGVYAGSVADRFDKRRILVWSNAGYAVLAALQFALVATDVVQIWM
                     VFVIAFLTGLILAIEPPTRQSFYVELVGEEHITNAISLNSAVFTGTRVLGGAIAGTMI
                     DTFGLWSPFLLDAVSYVGVIAALLAMRIHELHPQRKADREPGLVRAGLRYVWSSRALR
                     VPLIVMALVYLFTFNFSVVIPLQAHRTYEGSGMEIGWLYAALGLGAFLGAIAMANRQT
                     EPTLGRLAGFSIAFGALLAVAGWAPTFVLGLIAMVPVGYASMLFAITANSTLQRFTRA
                     DMRGRVMALYTTIFLGSSAIGGPIAGWVAEFWGVPEAIAASGAVAVGAGLFALRARET
                     AAPAGDPATVDTAARSA"
BASE COUNT          483 a         1060 c         1052 g          465 t
ORIGIN      
        1 gaaggcggcg ttggtggggc tcgtgctctt cgatccgacc gaccaagcgg tccccttctt
       61 cgcgggcgcg ctcctcatca tgtcggtgaa ccggttcttc ctgtcgaccg cggccgccgt
      121 cgtgccgcgg ctcgtcccgc acgaggacct cctcgtcgcg aactccctgg caacggtcgg
      181 tggcaccgtg gctttactcg ccggggtgtt cgtgggaggt cgcgtcatcg acgtcgtcga
      241 cgtcacggtc ccggtggtca tcgcagccgg cttcggctgg ctcgctgcct cctggatcgc
      301 gtcgcgcatc gtgagctcgc tcaagcccca cacgaagccc gaggatcccg agctcctccg
      361 gcaccagctg cggcgcgtcg gggtggagtt ctccgacggc gcgcggcgcc tgctccggag
      421 cccgcgtgcg atcggcccga tcgcgtccat caccctcgac cagatcggac aggggatcgt
      481 tctgacgctg tccctcgtgg tgttccgcca cgaattcggt gaaggggtcg cctcgttctc
      541 gaacctgatc ggcgccggtg gcgtcggcgt cctggtcggc atcctgacgg tgggcaagct
      601 cgaggaacgg ttctcgaagc cgaggatcgt ggccggagcg ttcgtggtgg gcggtgtcgt
      661 cctgttcgcc gtgtcgttcc tcctccggga ctggaccatc ctgctcgcga gcttcgccac
      721 tggactcacg ttcgcgtgga agaagatctc cgtggacacg atggtgcagg aggcggtccc
      781 cgacgggtac cgcggacgcg tgttcagcgt ctacgacgtc gtctacaacc tcgcgagggt
      841 catcgcggcg gcgctcgcga tcccgatgat ccctcgcctc ggcgtcgagg gatccctcgc
      901 gatcgtgggc atcgtgttcc tcctgtggcc cccggtgatc ggccggtggt acgggcggga
      961 gccggagctc gagatccgct tcgtcgccgg cgggcgcgcc gacgagacgc cacgcgcgat
     1021 ctcctggggc ggagcggagg agcccgtgga gctcgttcgg acgtggcggg aagagcggga
     1081 cgggcagcgg cggctcgcct tccgtctcgc cctcccggac ggtacgcagc tcgacgtcag
     1141 ccgccccgag ccggacgggc cctggcgtct cgatcgcgag gacgaccgtc ttccctccga
     1201 ctcggggggg agccgtatcg tgtccgggga tcggacggag ccaccatgaa cgagcggagc
     1261 ggacggaacg cattgatcgt cggcgcgatc gtcctcgccc tgctctcgat cggtgcggga
     1321 acgctgatcg cgctctccga cgacgacgag cgggcgagca cgccgacaac gacctcgagc
     1381 ctcacccctt cggccgagcc gagcgacgag cccaccgtcg ctcccgaccc gaccatgccc
     1441 gcgtccccgg aaccaccggg gtccgagtcc ccgattctcg aggacggcca ccacttcgtg
     1501 tacatcgccg gcgcttcccg tctggaggcc ggcacctcgg acgtggagtt cgacctggcg
     1561 tacttctacg ttggcggatc cgcggagcgg gaagccgccg aacgcgacga cgaggtcctg
     1621 gacggctact acatcgtgaa cgagaacgac cggctgcgga ccttgccgct cgccgaagac
     1681 gtcgaggtcg cgtacatccc cgtcgatgcg tgctgcgacc tccagcccgg gaacgtcgac
     1741 gcctggctcg aggcggtcct ccagacgaac cagaccgact acggcggcgc ggacgtcccg
     1801 tggtggttca cggtcgagag cggggagatc acgcggatcg agcagcagta tctaccgtag
     1861 ccggatcgcc cgcaggcgcc gcggtctctc gcgcccgcag agcgaacagc ccggccccca
     1921 cggcgaccgc gccgctcgcc gcgatcgctt cgggcacacc ccagaactcg gcgacccaac
     1981 cggcgatcgg ccctccgatc gcgctgcttc ccaggaagat cgtcgtgtac agcgccatca
     2041 cccttccgcg catgtccgcc ctggtgaacc gctgcagcgt cgagttcgcc gtgatggcga
     2101 acagcatcga ggcgtacccg acgggcacca tcgcgatcag tcccagcacg aacgtcggcg
     2161 cccatcccgc gacggcgagc aacgcaccga acgcgatcga gaagcccgcg agccggccga
     2221 gcgtcggttc cgtctgtcgg ttcgccatcg cgatggcgcc gaggaacgcc ccgagaccca
     2281 gcgcggcgta cagccagccg atctccatcc ccgagccttc gtaggtccgg tgcgcctgga
     2341 gcggtatcac aacgctgaag ttgaaggtga acaggtacac gagcgccatc acgatgaggg
     2401 gaacccggag cgcccgggag ctccacacgt accggagccc cgcgcggacg aggcccggtt
     2461 cacgatcggc cttgcgttgg gggtgcagtt cgtggatgcg catggccagc agggcggcga
     2521 tgaccccgac gtaggagacc gcgtccagga ggaagggcga ccagagcccg aacgtgtcga
     2581 tcatcgtccc ggcgatcgcg ccccccagca cgcgggtgcc ggtgaagacg gcgctgttca
     2641 ggctgatcgc gttcgtgatg tgctcctccc cgaccagttc cacgtagaag gactgccgcg
     2701 tcgggggctc gatcgcgagg atcaacccgg tcaggaacgc gatcacgaac accatccaga
     2761 tctggacgac gtccgtggcg acgagcgcga actggagggc ggcgagcacg gcgtagccgg
     2821 cgttcgacca gacgaggatg cggcgtttgt cgaaccggtc ggcgacagag ccggcgtaga
     2881 ccccgaagag gaggaccggg aggaacagga gcgccgcctc gatccccagc gccacgccac
     2941 tgtccgtgag cttgagcacg agcacggcac cggcggtcca gttcacccag gtgccggtga
     3001 cggagatcag ctgcccgacg aagaagaggc ggaagttcct cgagacgtgc atcgaggcga
//
LOCUS       DASUVE010000030         5744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1334130, whole genome shotgun sequence.
ACCESSION   DASUVE010000030 DASUVE010000000
VERSION     DASUVE010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334130"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /gene="carA"
                     /locus_tag="VF036_00595"
     CDS             complement(<1..1138)
                     /gene="carA"
                     /locus_tag="VF036_00595"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX6263756.1"
                     /translation="MTAPAALLALEDGTVFRGSGFGAEGESFGEAVFNTGMAGYQEVL
                     TDPSYAGQIVTMTAPQQGNYGMHAEHAESGAIQVAAFAVTEASRRSSSPRADSTLPEA
                     LLEAGVVAIEGIDTRKLTRRIREVGAMRAAVSTRDLDPVSLVARVRETGGMAGADLAR
                     TVTTAAPYEAGTLVGPADRARGRVWRVAAYDFGIKRNILRMLAAAGLDATVFPAETPA
                     ADVAAGGFDGVLLSNGPGDPAATSYGVAAARELLGRLPLFGICLGHQLLGLALGGRTF
                     KMPFGHRGVNQPVREMRTGEVRITSHNHGFAVDPAGWERDADGTARTAFGRVEVSHRN
                     LNDDTIEGLRCLDVPAFSVQYHPEAAPGPHDAAGLFLEFRSLIEA"
     gene            complement(1135..2439)
                     /locus_tag="VF036_00600"
     CDS             complement(1135..2439)
                     /locus_tag="VF036_00600"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019434750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX6263757.1"
                     /translation="MASLELLFRGARVIDPRAGRDEVADVHVRDGVIVDVRSGLDGPS
                     AEIVDGDGLVLTPGLVDLHTHLREPGFEQKETIETGTRAAAAGGYTAVAPMANTDPVA
                     DDASVIAEVRAAAARAGLADVFPVGAITKGLEGEVLAEIGEMAAAGVRVFSDDGRCVP
                     TARTLRNALTYARAFDDVVIAEHCEDASLSSGQQMHEGFSSSSLGLTGQPGAAEDAIV
                     YRDIELARLTGGRLHICHLSSARSVELVRRAKADGLRISAEVTPHHLVFIDEDLRTYD
                     TNKKVNPPFRTEADLDALREGLADGTIDAIATDHAPHAVEEKEAEFDRAPPGTIGLET
                     ALAVALTELVAPGLVTLPRALESLSSAPALILGARDHGGPIEAGRPANLVLFDPEERW
                     TVEPPFVSRSRNSAFIGRELRGRVRCTMLHGELTIAEGKATR"
     gene            complement(2432..3373)
                     /locus_tag="VF036_00605"
     CDS             complement(2432..3373)
                     /locus_tag="VF036_00605"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX6263758.1"
                     /translation="MNEHLLSMHDVTADDVIRILDTAESFREVGTRVIKKVPALRGRT
                     VVNLFYENSTRTRISFELAAKRLSADVINFSSGGSSVSKGESLKDTALTLQAMGADAI
                     VIRHSSSGSPFQLTRWVRGHVLNAGDGTHEHPTQALLDLYTMRERLGRLEGLRVAIVG
                     DVLHSRVARSLSFGLVKMGADVTLVGPATLIPPDAPQWGVQVSYDLDEVLAKLDVCYL
                     LRVQTERQRLEYFPSVREYARLFGLNRSRVGLLPAGSLIMHPGPMNRGVEIDSDVADL
                     PQSVIEDQVTNGIAVRMALLYLLLGGRRGDGEEAGDG"
     gene            complement(3370..3951)
                     /gene="pyrR"
                     /locus_tag="VF036_00610"
     CDS             complement(3370..3951)
                     /gene="pyrR"
                     /locus_tag="VF036_00610"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006692635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX6263759.1"
                     /translation="MSRILGPDDVRRALARIAHEIVERERTEGLVLVGIADRGDHLAL
                     RLAREIAAIEGAEIPVGVLDITFYRDDIGMTAEAPEVHETRIPFDITGRTVILVDDVL
                     FTGRTIRAAMDALTDLGRARRIRLAVLVDRGHRELPIRADFVGKNVPTQLGDDVRVLV
                     HEVDGEDGALVRDGPTGRALREAPAVAGQEESA"
     gene            4159..4407
                     /locus_tag="VF036_00615"
     CDS             4159..4407
                     /locus_tag="VF036_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6263760.1"
                     /translation="MPDWGWIVLVVAVVFGASKLPDIARNLGRSSGEFKKGLKEGNEG
                     EEDPMAAPPPSSGVDPTAAAPPLRSTPESDQRQPGTPP"
     gene            complement(4404..4829)
                     /gene="nusB"
                     /locus_tag="VF036_00620"
     CDS             complement(4404..4829)
                     /gene="nusB"
                     /locus_tag="VF036_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX6263761.1"
                     /translation="MTSRREARRTAIDILYQADVTAADPSLVLGEWLDAGRVVSAFTT
                     ELVEGVQDHLPEIDLLLEERTEGWTVARMAALDRTILRVAVHELRHRADVPASVAINE
                     AVEAATDLSSEGSPRFVNGILGKIASEMRPAAAEDPGTA"
     gene            complement(4826..5392)
                     /gene="efp"
                     /locus_tag="VF036_00625"
     CDS             complement(4826..5392)
                     /gene="efp"
                     /locus_tag="VF036_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX6263762.1"
                     /translation="MSVSTNDLKNGMTLELEGTLFQVIEFQHVKPGKGGAFVRTKLRN
                     VKTGAVADKTFNAGVKVGLAIVERKEMQYLYADGPDLVFMNLQTYEQVHVPGDIVGEA
                     TRYMTEGGQATVALHQGVPISVDLPASMTLAVAQTDPATKGDTRTNALKPATLETGTV
                     VQVPLFVEEGDRVKVDTRSGGYIERVKA"
     gene            complement(5397..>5744)
                     /locus_tag="VF036_00630"
     CDS             complement(5397..>5744)
                     /locus_tag="VF036_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX6263763.1"
                     /translation="KVHDVVAAAQQAGIDALRPGVRAADVDHASRSVIEDAGYGNRFT
                     HGLGHGIGLEIHEGPSLRRGGDDIVPAGAVVTVEPGVYLPGLGGVRIEDAVEVGEEGP
                     NPLGVSTRELIEL"
BASE COUNT          821 a         2054 c         1984 g          885 t
ORIGIN      
        1 gcgcctcgat cagcgaccgg aactcgagga agagaccggc ggcgtcgtgc ggcccgggcg
       61 cggcctccgg atggtactgg accgagaacg cggggacgtc gaggcagcgc aatccctcga
      121 tcgtgtcgtc gttgaggttc cggtgactca cctccacccg gccgaacgcg gtccgggcgg
      181 tcccgtccgc gtctcgctcc cagccggcgg gatcgaccgc gaagccgtgg ttgtggctcg
      241 tgatgcggac ctcgcccgtc cgcatctcgc ggacgggctg gttcacgccc cggtgtccga
      301 agggcatctt gaacgtgcga cctcccagcg cgaggccgag caactggtgg ccgaggcaga
      361 tcccgaagag cgggagccgg ccgagcaact ccctcgcggc ggcgacgccg taggacgtcg
      421 ccgcggggtc gccgggaccg ttcgacagca ggaccccgtc gaagccgcct gccgcgacgt
      481 ccgcggcggg cgtctccgcc gggaagacgg tggcgtcgag acccgccgcc gccagcatcc
      541 gcaggatgtt ccgcttgatc ccgaagtcgt acgcggcgac ccgccatacc cgtcctcggg
      601 cgcgatcggc gggacccacg agcgtcccgg cctcgtacgg tgcggcggtc gtgaccgtcc
      661 tcgcgagatc ggcaccggcc atcccgccgg tctctcggac gcgggccacc aacgagacgg
      721 gatcgaggtc ccgcgtcgag acggccgcgc gcatcgcgcc gacctcgcgg atccggcggg
      781 tgagtttgcg cgtgtcgatg ccctcgatcg cgaccacgcc ggcctcgagg agggcctccg
      841 ggagggtcga gtcggcgcgg ggactcgacg aacgccggga ggcttccgtg accgcgaacg
      901 cggcgacctg gatcgcgccg ctctccgcat gctcggcgtg catcccgtag ttgccttgct
      961 gcggagccgt catcgtcacg atctggccgg cgtaggacgg atccgtgagg acctcctgat
     1021 agccggccat cccggtgttg aagaccgctt ctccgaacga ctcgccctcc gcgccgaatc
     1081 ccgatccccg gaagacggtg ccgtcctcga gggccaggag cgctgcgggc gccgtcaccg
     1141 cgtggccttc ccctccgcga tcgtgagctc cccatggagc atcgtgcagc ggacccgtcc
     1201 gcggagctcc cgaccgatga aggcgctgtt cctcgatcgg gagacgaacg gcggctcgac
     1261 ggtccagcgc tcctcgggat cgaacagcac gaggttggcg ggtctcccgg cctcgatcgg
     1321 gcccccgtgg tcccgggcgc cgaggatcag ggccggcgcg gacgacaggc tctccagcgc
     1381 cctcggcagg gtgacgaggc cgggcgcgac gagctccgtg agggccaccg cgagcgccgt
     1441 ctcgagcccg atcgtgccgg gaggcgcccg gtcgaactcg gcctccttct cctccaccgc
     1501 gtgcggtgcg tgatcagtgg cgatcgcatc gatcgtgccg tcggcgagtc cctcacggag
     1561 cgcgtcgagg tccgcctccg tgcggaacgg cggattcacc ttcttgttcg tgtcgtaggt
     1621 gcgaaggtcc tcgtcgatga acacgaggtg atgcggcgtg acctcggccg agatccggag
     1681 cccgtccgcc ttcgcgcgcc gcacgagctc caccgacctc gccgacgaca ggtggcagat
     1741 gtgcaggcgg ccgccggtga ggcgggccag ctcgatgtcg cggtagacga tcgcgtcttc
     1801 ggccgcaccc ggttggcccg tgaggccgag ggaggaggag ctgaacccct cgtgcatctg
     1861 ctgcccgctg gacagggagg catcctcgca gtgctccgcg atcacgacgt cgtcgaacgc
     1921 ccgcgcgtag gtcagggcgt tccggagcgt gcgggcggtc gggacgcatc ggccgtcgtc
     1981 gctgaacaca cgcacacccg cggccgccat ctcgccgatc tcggccagca cctcgccctc
     2041 gagccccttc gtgatcgcgc cgaccgggaa gacgtccgcg agcccggcgc gggcagcggc
     2101 ggcccggacc tcggcgatca cggaggcgtc gtcggccaca ggatccgtat tggccatcgg
     2161 cgcgaccgcc gtgtacccgc cggccgcggc cgcccgggta cccgtctcga tcgtctcctt
     2221 ctgctcgaac cccggttcgc gcagatgggt gtggaggtcc accaatcccg gtgtcagcac
     2281 gagcccgtcg ccatcgacga tctcggcgga ggggccgtcg aggccactcc ggacgtcgac
     2341 gatgaccccg tcccggacgt ggacgtcggc gacctcgtcg cgaccggcgc gggggtcgat
     2401 cacgcgcgcg cctcggaaca gcagttcgag gctagccatc gcccgcctcc tcaccgtcgc
     2461 cgcgccgccc gcccagcagt aggtacagca gcgccatccg gaccgcgatg ccgttcgtca
     2521 cctgatcctc gatcacgctc tgcggcaggt cggcgacgtc ggagtcgatc tcgacccccc
     2581 ggttcatcgg gcccggatgc atgatcagcg accccgccgg gaggagcccc acccgcgatc
     2641 ggttcaatcc gaacaggcgc gcgtactcgc gcaccgaagg gaagtactcg agtcgttgcc
     2701 gttcggtctg cacgcgcagc aggtaacaga cgtcgagctt cgccaggacc tcgtcgaggt
     2761 cgtagctcac ctggacgccc cattgcggcg cgtcgggagg gatcagggtg gccgggccca
     2821 cgagcgtcac gtcggcgccc atcttcacga gaccgaacga gagcgaccgg gccactcggg
     2881 agtgcaggac gtcccccacg atcgccacgc ggagcccttc gagccgcccg agccgttcgc
     2941 gcatcgtgta caggtcgagc agcgcctggg tgggatgctc gtgcgtgccg tctcccgcgt
     3001 tcagtacgtg tccgcgtacc cacctcgtca gctggaacgg cgagcccgag gacgagtggc
     3061 ggatcacgat cgcgtccgcg cccatagcct gcagcgtgag cgccgtgtcc ttcagtgact
     3121 cgcccttcga tacgctcgag ccgcccgatg agaagttgat cacgtccgcg gacaggcgct
     3181 tggccgccag ctcgaacgag atccgcgtac gcgtggagtt ctcgtagaag agattcacca
     3241 cggtgcgacc acggagtgct gggaccttct tgatcacccg ggtcccgacc tcccggaagg
     3301 actcggccgt gtcgaggatc cggatcacgt cgtccgcggt cacgtcgtgc atcgacagga
     3361 ggtgctcgtt cacgcgctct cctcttgacc ggcgacggcc ggggcctccc ggagcgctcg
     3421 tcccgtcggc ccgtcccgca ccagggcccc gtcctcgccg tccacctcgt gcaccaacac
     3481 acgcacgtcg tcaccgagct gggtggggac gttcttcccc acgaagtccg cccggatcgg
     3541 cagctcgcga tgaccgcgat cgacgaggac ggcgagtcgg atccgccggg cgcgaccgag
     3601 atcggtcagc gcgtccatcg cggctcggat cgtcctcccg gtgaacagga cgtcgtcgac
     3661 gaggatcacg gtgcgccccg tgatgtcgaa cgggatcctg gtctcgtgga cctcgggcgc
     3721 ctccgccgtc atcccgatgt cgtcgcgata gaacgtgatg tccaggacgc cgaccgggat
     3781 ctctgcgccc tcgatcgcag cgatctcgcg ggcgaggcgg agcgcgaggt ggtcgccccg
     3841 gtcggcgatg ccgaccagga ccaggccctc ggtccgctcg cgctccacga tctcgtgggc
     3901 gatccgggcg agcgctcgac ggacgtcgtc tggtccgagg atcctgctca cacgggaacg
     3961 ccccctcgag caggcgagga ggcgcgcagg ttgtgccgca tcggttggct cccttcccgg
     4021 cctcacggga ccggacttaa aggggaagtg cagccggagg ataccctggt gtcggcccgc
     4081 gggcaacgaa cgtgacatcc tgccctcgat ggacctcggc gtagactgtg ggataccgag
     4141 cggataggag cggttgagat gcctgattgg ggatggatcg tgctggtggt ggccgtcgtc
     4201 ttcggtgcga gcaagctgcc cgacatcgcg cggaacctcg gccggtcctc gggtgagttc
     4261 aagaagggcc tcaaggaggg gaacgagggc gaggaagacc cgatggcggc accgccgccg
     4321 tcctcaggcg tggaccccac cgccgccgcg ccgccgttgc gctcgacccc ggagtcggat
     4381 cagcgtcagc cgggcacgcc tccctaggcg gtccccgggt cctcggccgc ggcgggtcgc
     4441 atctcgctcg cgatcttccc gaggatcccg ttcacgaagc ggggcgaccc ttcggaggag
     4501 agatcggtcg ccgcttccac ggcctcgttg atcgcgaccg aggcgggcac gtcggcgcga
     4561 tgtctgagct cgtggaccgc cacccggagg atcgtccgat cgagcgcggc catccgcgcc
     4621 acggtccacc cctccgtgcg ctcctcgagg aggagatcga tctccgggag gtgatcctgg
     4681 accccttcca ccagctcggt cgtgaacgcg gagacgaccc ggccggcatc gagccactcc
     4741 cccagcacca ggctcgggtc tgcggcggtc acgtctgcct gatacaggat gtcgatcgcc
     4801 gtccggcgag cctcgcgacg cgaggtcacg ccttcacgcg ctcgatgtat ccgcccgagc
     4861 gcgtgtccac cttcacgcgg tcgccctcct ccacgaagag gggcacctgc accacggtgc
     4921 cggtctcgag ggtggccggc ttcagggcgt tcgtcctggt gtctcccttg gtggcgggat
     4981 ccgtctgcgc gaccgccagc gtcatcgagg cgggcaggtc caccgagatc ggcacaccct
     5041 gatggagcgc cacggtcgcc tggccgccct cggtcatgta gcgggtcgcc tcccccacga
     5101 tgtcgccggg gacgtgcacc tgttcgtagg tctgcaggtt catgaagacg agatcggggc
     5161 cgtccgcgta gaggtactgc atctccttgc gttcgacgat cgcgaggccc actttgacgc
     5221 cagcgttgaa cgtcttgtcc gcgacggcgc ccgtcttgac gttgcggagc ttcgtgcgca
     5281 cgaatgcgcc acccttgccc ggcttcacgt gctggaactc gatcacctgg aagagggtcc
     5341 cctcgagctc cagcgtcatg ccgttcttca ggtcgttggt ggagacgctc atcccgctac
     5401 agctcgatca gctctcgcgt ggagaccccg agcgggttgg ggccttcctc gccgacctcg
     5461 acggcgtcct cgatgcgcac gcccccgagg ccggggaggt acacgcccgg ttcgactgtg
     5521 accacggccc cggcgggcac gatgtcgtcg ccgccgcgcc gcagggacgg cccctcgtgg
     5581 atctcgaggc cgatcccgtg gccgagcccg tgggtgaatc ggttgccgta gccggcgtcc
     5641 tcgatgacgc tgcgggacgc gtggtcgaca tcggcggcgc gcacgcccgg acgcagtgcg
     5701 tcgatccccg cctgctgcgc ggccgcgacg acgtcgtgta cctt
//
LOCUS       DASUVE010000031         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1146781, whole genome shotgun sequence.
ACCESSION   DASUVE010000031 DASUVE010000000
VERSION     DASUVE010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146781"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VF036_00635"
     CDS             <1..586
                     /locus_tag="VF036_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6263764.1"
                     /translation="EGLDAVSMRRVAREVGVEAMSLYHHVEDKEDLLDGICEHVMAGF
                     EFPDPVEDWAENCRRGARAWRRLLQSHPAVMRLFAEQRGPVRTVDSMRPMEFALRLLR
                     GAGLSDRDTAQAFHAFGGYIQGFVMMELGSIAGGSDEAHLKAHADLVSALPEEFEALV
                     AVSPYFAECDADEQFEFGLDLLIRGLEAKAARTG"
     gene            653..1507
                     /locus_tag="VF036_00640"
     CDS             653..1507
                     /locus_tag="VF036_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263765.1"
                     /translation="MKVPLLAALALLFTLAACADSPVDPGAGGIDHSTAPDVALVQIS
                     YEGGFVPVEWTYTSLPFYSLFGDGTLVVPGAQIEIYPGPALPAISSRQVDEAGIQAIL
                     EQAVAVIRNIPVDLNDLGFMNVADASTTVITLSAGGVDRTIRAYALSELTERPDGMAE
                     REYQARLSLQELVATLTGPTDWLPEGSLGPESEYAASSARVYVGDHRTVDDLAQDPMP
                     WPLSAGLADFGDPIPPGEMRCGVVEGEEWDTLHEAAAAANQLTPWTDAGDRYSILFRP
                     LLPDEQGC"
     gene            1614..>2206
                     /locus_tag="VF036_00645"
     CDS             1614..>2206
                     /locus_tag="VF036_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6263766.1"
                     /translation="MTNEIEDSAVDRPRTTDGFFEPPAVGSADRTRELELRYRGIIDR
                     LPAVLYVDAVDQNQPMIDVSPSVHDLLGIPREEFLSRPFAWTDTIHPDDLAAVMAENE
                     RSAVTGDPFRMEFRVVHPDGRILWVRDDAVLIHDESGAPLHWLGLMLDVTELIEAQAE
                     LHEAKTKYGALVEQIPAIVYVDAADDEMSTTYVSPQI"
BASE COUNT          352 a          788 c          730 g          336 t
ORIGIN      
        1 ggaaggcctc gacgccgtct cgatgcggcg ggtcgctcgc gaggtcggcg tcgaagcgat
       61 gtcgctctac caccacgtcg aggacaaaga agacctgctc gatgggatct gcgagcacgt
      121 catggcgggg ttcgaattcc ccgatcccgt cgaggactgg gcggagaact gccggagagg
      181 agcccgagcg tggcgccgcc tcctgcagtc gcatcccgcc gtgatgcgcc tcttcgccga
      241 gcaacgagga ccggtccgca cggtggactc gatgcgcccg atggagttcg cgctcaggct
      301 cctccgcggc gccggcctct ccgaccgtga caccgcgcag gcgttccacg cgttcggcgg
      361 ctacatccag gggttcgtga tgatggagct cggctccatc gcgggcggat ccgacgaggc
      421 gcacctgaag gcccacgccg acctggtgtc ggccctcccg gaagagttcg aggcgctcgt
      481 ggcggtatcg ccatacttcg ccgaatgcga cgccgacgag cagttcgagt tcggcctcga
      541 tctcctgatc aggggcctcg aggccaaagc tgctcggacc ggctgacgcc gacgagggaa
      601 cctgactcgg acgatccgcg tccaagtccg aaccgccgac acggaggaac ccatgaaggt
      661 tccgcttctc gccgctctcg cgctcctgtt cacgctcgcc gcctgtgccg actcgccggt
      721 cgatccgggt gccggcggca tcgaccactc caccgccccc gacgtggcgc tcgtccagat
      781 ctcgtacgag ggcggcttcg tccccgtcga atggacctac acgagcctcc ccttctactc
      841 cctcttcggc gacggcaccc tcgtcgtccc cggcgctcag atcgagatct atccgggccc
      901 cgcgctgccc gcgatctcca gccggcaggt tgacgaagcg gggatccagg cgatcctcga
      961 gcaggcggtc gcggtgatca ggaacatccc cgtcgacctg aacgatctcg gcttcatgaa
     1021 cgtcgccgac gcctcgacga cggtgatcac cctctcggcc gggggcgtgg atcggacgat
     1081 ccgtgcgtac gcgctgagcg agttgacgga gcgccccgac gggatggccg agcgcgagta
     1141 ccaggcgcgc ctcagcctcc aggagctcgt cgccacgctg acgggtccga ccgactggct
     1201 ccccgagggg tcgctcggcc ccgagagcga gtacgcggct tcctcggcac gtgtgtacgt
     1261 cggggatcac cgcaccgtgg acgacctggc ccaggatccg atgccgtggc cgctcagcgc
     1321 cggcctcgcg gacttcggcg atccgatccc gcccggcgag atgcgctgcg gcgtcgtcga
     1381 gggtgaggaa tgggacaccc tccacgaggc ggcggcggcc gcgaaccagc tcaccccgtg
     1441 gaccgacgcc ggcgaccggt actcgatcct gttccgaccc ctgctcccgg acgagcaggg
     1501 ctgctgagcc ccttccgtcc atgaggagct gagcggccgg tcaagtcccc tcctcgtgga
     1561 gccgatggtt ccgtgaggac caggccgccc tcccgcggcc tctgtgccaa cccatgacga
     1621 atgagatcga ggactccgcc gtggaccgac cgaggaccac cgacggcttc ttcgagccgc
     1681 ccgccgtggg aagcgccgat cgtacgcgcg agctcgagct ccgctatcgc gggatcatcg
     1741 accggctgcc ggccgtgctc tacgtggacg ccgtcgacca gaaccagccg atgatcgatg
     1801 tgagcccgag cgtccacgac ctgttgggga tcccccgtga ggagttcctg agcaggccgt
     1861 tcgcgtggac cgacacgatc caccccgacg acctcgccgc ggtcatggcg gagaacgagc
     1921 gctccgcggt caccggagac ccgttccgca tggagttccg cgtcgtccac cccgacggga
     1981 ggatcctctg ggtccgcgac gacgcggtcc tgatccacga cgagtcggga gcaccgctcc
     2041 actggctggg gttgatgctc gacgtcacgg aactgatcga ggcgcaagcc gagctccatg
     2101 aggccaagac gaagtacggc gccctggtgg agcagatccc ggcgatcgtc tacgtcgacg
     2161 cggccgacga cgagatgtcg acgacgtacg tgagccccca gatcca
//
LOCUS       DASUVE010000032         4208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1524641, whole genome shotgun sequence.
ACCESSION   DASUVE010000032 DASUVE010000000
VERSION     DASUVE010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524641"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VF036_00650"
     CDS             <1..262
                     /locus_tag="VF036_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263767.1"
                     /translation="LLHDARPVLLNLREPGSFDVAPWADRVQAIDAGYVGPWELPAIG
                     TVTAPIALLIRPDGHVAWVGEDITGRGLVDALTTWFGPPTAA"
     gene            complement(312..1517)
                     /locus_tag="VF036_00655"
     CDS             complement(312..1517)
                     /locus_tag="VF036_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX6263768.1"
                     /translation="MPPHQVAVRRRSVAFAAALLAVVAGFLLWPSQDAAPVRPDLGEG
                     TISPYSGPDNYLAWVPGGFDDPAFRQKMERLPGLDEAVVLAGDTLWLRRTEDAQGVVV
                     DEPKNPFAFPIDAFAVDANDYAPFVGASVREEIVRALGAGRAVLGEHSASLRRLGPGG
                     RLVFRTGSVRVGAVVPDDAVGWSEVLLNRDVGRQLGIAHERYLLAQPSEELTQPVWKR
                     KLIPFVGDDPLRVDVAGSTRYVRVASGVRPPILVKQRFGEFAATPQADPAYLTIDPAW
                     VERNIVSTEVPLLGTVTCHRKLIPMVRGALYEIAASSLASEIKVYSGCWASRTVSRSP
                     TAPPSYHAYGAAIDINAPQNPYGTKPTMNPEIVRIFGSWGFNWGGDFLIPDGHHFEFW
                     RIPDQLRAT"
     gene            complement(1538..4018)
                     /locus_tag="VF036_00660"
     CDS             complement(1538..4018)
                     /locus_tag="VF036_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6263769.1"
                     /translation="MTERMLDDTLTATSFAPDAADGPLPDRAQVVVIGGGIAGSSIAY
                     HLAGLGITDVLLLERARIASGTSWHAAGLVARVRGSHPMTKLANYGVDLYRGLGEETG
                     VDVHFRPTGSLTLAENEGRVTELRYAAAIARHHGTEARLLEPSEVPEVWPLISTDGLV
                     GALLQPGDGTVNPGYAALALAKGAHDRGATIREGVRVEELLLDGRTMTGVRTDRGDVE
                     AETVVLACGLWTRDLAATVGVPVPLYAAEHVHVSTDPIEGAHDLLPLVRGLDGSFYAR
                     HHAGGLMIGAFEPNGRPRSTASISEGWAFGEFGPDWEHFAPTRVNAARRIPALEDAEF
                     TRFLRAPESFTPDVNFCLGETAEVRNLFVAAGFNSQGIIYSPGAGRALAEWIREGAPA
                     FDASEVDVRRFASSQANERYLHERTKEALGRLYAMHWPNLQATTARGIRRSPLYDRLD
                     AAGACFGELNQWERPMWFAPAGVRPEYAYSFGRQNWFPYAAEEHRAAREAVALFDLSS
                     FAKFEVTGADALRLLQRVCTNDVDGRTGRLVYTLFLNLSGGIENDGTVTRLAADRFLV
                     ITPTATQHRTLEWLRAEADGMAASIDDVTSAFATIALMGPHSRELLGSIASADLSNEG
                     FPFFSAQEIQVASAPVLVIRASFVGELGWELYVPSEFAVHVYDTILEAGKGFGLRHAG
                     YHALDSLRIEKGFVHVGHDVGPTDDPFSAGLGHVVKLAKDFVGAEAARRAKDTPGSHR
                     LASLTLDDPEPILLHGESVIADGKVVGTVMSAAYAHTIGAAVGLAMVEASVVADETPV
                     QVDIAGEMVKATLSLSPLYDPSGARMRA"
BASE COUNT          639 a         1478 c         1479 g          612 t
ORIGIN      
        1 cctgctgcac gatgcccggc cggtgctcct caacctccgc gagcccggct ccttcgacgt
       61 cgctccatgg gcagatcgcg ttcaggcgat cgacgccgga tacgttgggc cgtgggagct
      121 gccggcgatc gggacggtca ctgctcccat cgccctcttg atccgccccg acggacatgt
      181 ggcctgggtg ggagaggaca tcaccgggcg cgggctcgtg gacgcgctca ccacctggtt
      241 cgggccgccg accgcggcgt agcgctccgg tcggatcatg ctgcgactgg acgtggatcg
      301 ggagacgtgt cctaggtcgc tcgaagctgg tccgggatgc gccagaactc gaagtggtgg
      361 ccgtcgggga tcaggaagtc gcctccccag ttgaagcccc agctcccgaa gatccggacg
      421 atctccgggt tcatcgtcgg cttcgttccg tacgggttct gcggtgcgtt gatgtcgatc
      481 gcggcgccat acgcgtgata cgaaggcggc gccgtggggc tccgcgagac cgtgcgagac
      541 gcccagcatc cggagtagac cttgatctcc gacgcgaggc tcgatgcggc gatctcgtag
      601 agcgcgcccc ggaccatcgg gatcagcttc cggtgacacg tgacggtccc gagcagcggc
      661 acctcggtcg agacgatgtt ccgttcgacc caggcggggt cgatcgtcag gtacgccggg
      721 tcggcctgcg gcgtcgcggc gaactcgccg aagcgctgct tcacgaggat cggggggcgc
      781 acaccgctcg cgacccgcac gtacctcgtc gagcccgcga cgtcgacgcg gagggggtca
      841 tcacccacga aggggatgag cttccgcttc cagaccggtt gtgtgagctc ctcggacggc
      901 tgcgctagga ggtaccgctc gtgcgcgatc cccagctggc ggccgacgtc gcggttcagg
      961 agcacctcgg accacccgac cgcgtcgtcg gggacgaccg cgccgacccg gaccgatccg
     1021 gtccggaaca cgagcctgcc gcccgggccg aggcggcgga gactcgcgct gtgctcaccc
     1081 agcaccgccc ggccggcacc gagcgcccga acgatctcct cgcggacgct cgcgccgacg
     1141 aacggcgcgt agtcgttcgc gtccaccgcg aaggcgtcga tcgggaacgc gaacgggttc
     1201 ttgggctcgt ccaccaccac tccctgggca tcctcggtcc tgcgcagcca cagcgtgtcg
     1261 ccggccaaca ccaccgcctc atcgaggccg ggcaggcgtt ccatcttctg gcggaaggcg
     1321 ggatcgtcga agccccccgg gacccacgcg aggtagttgt cgggccccga gtagggcgag
     1381 atcgttccct cgccgaggtc cgggcgaacc ggtgcagcat cctgcgacgg ccagagcaag
     1441 aacccggcga cgacggcgag gagcgcggcc gcgaacgcga ctgagcgccg ccggacggcg
     1501 acctggtgtg ggggcacgtc ttccaggcta cttcgggtca ggcacgcatc cgagcaccgc
     1561 tcgggtcgta gagcgggctg agggagagcg tggccttcac catctcgcca gcgatgtcca
     1621 cctggacagg tgtctcgtct gcgacgaccg aggcctccac catcgccagc ccgacggccg
     1681 cgccgatcgt gtgcgcgtag gccgcggaca tcaccgtgcc cacgaccttc ccgtcggcga
     1741 tcacggactc gccgtggagg aggatcggct ccggatcatc gagcgtgagg ctcgcgagtc
     1801 gatgcgatcc cggcgtgtct ttcgcgcggc gagccgcctc ggcaccgacg aagtccttcg
     1861 ccagcttcac gacatgtccg agaccggcgg agaacgggtc gtcggtcggg ccgacgtcgt
     1921 ggccgacgtg cacgaacccc ttctcgatcc ggagcgagtc gagcgcgtgg tagccggcgt
     1981 gccggaggcc gaaccccttc cccgcctcga ggatcgtgtc gtacacgtgc acggcgaact
     2041 ccgacggcac gtacagctcc cagccgagct caccgacgaa cgaggcccgg atcacgagga
     2101 ccggcgcgct cgcgacctgg atctcctgcg ccgagaagaa cgggaatccc tcgttcgaga
     2161 ggtcggccga ggcgatgctt cccagcagct cgcggctgtg cggtcccatc agggcgatcg
     2221 tggcgaacgc gctcgtcacg tcgtcgatcg acgcggccat gccgtcggct tccgcacgga
     2281 gccactccag cgtccggtgc tgcgtcgcgg tcggcgtgat cacgaggaac cggtcggcgg
     2341 ccaggcgcgt gaccgtcccg tcgttctcga tcccaccact gaggttcagg aagagggtgt
     2401 agacgaggcg cccggtacgc ccatcgacgt cgttcgtaca cacccgttgg agcaggcgca
     2461 gggcgtccgc gcccgtcacc tcgaacttgg cgaacgagct caggtcgaag agcgccaccg
     2521 cctctcgcgc cgcgcggtgc tcctcggccg cgtatgggaa ccagttctgc cggccgaagg
     2581 agtacgcgta ctccggccgg acaccggccg gcgcgaacca catcgggcgt tcccactggt
     2641 tgagctcgcc gaagcaggca cccgctgcgt ccaatcggtc gtagagggga gagcgccgga
     2701 tgccgcgcgc ggtggtcgcc tggaggttcg gccagtgcat cgcgtagagc cggccgagcg
     2761 cctccttcgt ccgctcatgg aggtagcgct cgttcgcctg cgacgatgcg aaacgccgca
     2821 cgtcgacctc ggaggcgtcg aacgcgggag cgccctcccg gatccactcg gcgagggcgc
     2881 gacccgcccc cggcgagtag atgatcccct gcgagttgaa ccccgcggcc acgaagaggt
     2941 tccggacctc ggcggtctcg ccgaggcaga agttcacgtc cggggtgaag gactcggggg
     3001 cgcggaggaa ccgcgtgaac tccgcgtctt cgagcgcagg gatccgccgc gccgcgttca
     3061 ctcgcgtggg cgcgaagtgc tcccagtcgg ggccgaattc gccgaacgcc cacccctcgg
     3121 agatcgatgc ggtcgagcgc gggcgtccgt tcggctcgaa cgcgccgatc atcaacccgc
     3181 cggcgtggtg ccgcgcgtag aaggacccgt cgagtccgcg cacgagcggc agcagatcgt
     3241 gcgcgccctc gatcggatcg gtcgagacgt ggacgtgctc cgccgcgtag agcggcaccg
     3301 gtacgccgac ggtggcggcg aggtcacgcg tccacagacc acaggcgagg acgaccgtct
     3361 cggcctcgac gtcgccccta tcggtgcgaa cgcccgtcat cgtccggccg tcgaggagca
     3421 gctcttcgac ccgaacgccc tcgcggatcg tcgcgccgcg gtcatgcgca cccttcgcga
     3481 gggcgagcgc cgcgtatcca gggttcacgg tcccgtcgcc gggctgcagg agcgcgccga
     3541 cgaggccgtc tgtcgagatc agcggccaca cctcgggcac ctcggagggt tccaggagac
     3601 gggcctcggt cccgtggtga cgcgcgatcg cggccgcgta ccgcagctcc gtcaccctgc
     3661 cctcgttctc cgcgagcgtg agcgaacccg tcggccggaa gtgcacgtcg acgcccgtct
     3721 cctcgccgag ccctcgatag agatccacgc cgtagttcgc gagcttggtc atcggatggg
     3781 agccgcggac ccgggcgacg agccccgccg cgtgccacga tgtccccgag gcgatccggg
     3841 ctcgttccag gagcaggacg tccgtgatcc cgagcccagc gagatggtac gcgatcgacg
     3901 accccgcgat cccgcccccg atgacgacga cctgagcccg gtccggaagc ggcccatcgg
     3961 ctgcatcggg agcgaacgac gtcgcggtga gggtgtcgtc cagcatccgc tccgtcatga
     4021 ggtccgaagg ataccgacgc tccgcccggc tcccggttgc ctcgggtcgg gccggttggg
     4081 acgatgctgg cgtgagcgag gcgggaacgg cgatgcggga ggagcggcgg ttcgtaaccg
     4141 cgctgttcgc cgacctcgtc gggtcgacgg ccctcgggga gcggctcgag ccggaggagt
     4201 tgaagctc
//
LOCUS       DASUVE010000033         2590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1149188, whole genome shotgun sequence.
ACCESSION   DASUVE010000033 DASUVE010000000
VERSION     DASUVE010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149188"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VF036_00665"
     CDS             <1..313
                     /locus_tag="VF036_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6263770.1"
                     /translation="PLPEARVSFYRIAQEGVTNARKHANATHVDVRLTSLEGGLLLRI
                     VDDGDGFDPSERAAPGHLGLATVTERAELAGGWCRVLSAPGEGTVLECWMPLIASVPA
                     S"
     gene            320..1582
                     /locus_tag="VF036_00670"
     CDS             320..1582
                     /locus_tag="VF036_00670"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6263771.1"
                     /translation="MERWRAGRVWLAMWTIDGFLFHLDSTVYSVFLILQVGLDPLQLV
                     LMGTILEVSYLLFEVPTGIVADTVSRKWSIVIGYVGTGIAFVMLGSADSFGAAALSQV
                     LYGVSATFVSGADVAWLTDEVGEDAARPLYVRSEQFFNGGALVGIAGCVALASIALRL
                     PILVCGLGYIALGLSLAVGMTETRRPTRDAGAKLRHSMHVTMRDAIQQVRAHHILLLI
                     LATAVLHGASTEGWDRLSDLQLLSGIGLPSLGDLEPIVWFGILDGVGLVLGIAALSYV
                     RRRGHLEGHGHVAKLLALVDVSLIVSVVWFAFAGSFWWAAILFWIVGGLRSVRGPVFT
                     AWINQGLDPATRATINSMGGQADAVGQAMAGPVVGGVGRAVSVPWALTLAGLLRLPIL
                     LLYLRAIRRGTVGTVAPDAMDQEIDLAD"
     gene            complement(1558..1965)
                     /locus_tag="VF036_00675"
     CDS             complement(1558..1965)
                     /locus_tag="VF036_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6263772.1"
                     /translation="MGRLTAAWWAWRNRREVGRWLGFAWRAVPPSAADRGDLVAEARL
                     RTALAREPRTRGARSLTVRVHARTAFLGGNLPSDVHDLAYAIARRTAGVLRVECGIRD
                     RGRRRAPETHHHHGDVAALPPPPPERQSARSIS"
     gene            2135..2485
                     /locus_tag="VF036_00680"
     CDS             2135..2485
                     /locus_tag="VF036_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX6263773.1"
                     /translation="MFVIEMWTYRTDVVLQPAVDLVGFGIEARDGSIGKVDESTADAG
                     KSYVVVDTGPWIFGRKVLLPAQTVERIDLDARTVFVDRTKEEIKNAPEYDPERGIDPT
                     YQDELGGYYGRYYS"
BASE COUNT          370 a          871 c          906 g          443 t
ORIGIN      
        1 gccgcttccc gaggcccgag tgtccttcta tcggatcgcg caggaaggcg tcaccaacgc
       61 ccggaagcac gccaacgcca cccatgtgga cgtccgattg acctcgttgg agggcggact
      121 cctgctgagg atcgtggacg acggcgacgg gttcgatcct tccgagaggg cggcgccggg
      181 tcacctcggc ctggcgaccg tgaccgagcg tgccgagctc gccggtgggt ggtgtcgcgt
      241 gctcagcgca cccggtgaag ggaccgtcct cgaatgctgg atgccgttga tcgcgtccgt
      301 cccggcgtcc tgacgtccca tggaacggtg gcgggccggg cgggtctggc tcgcgatgtg
      361 gacgatcgac ggcttcctct tccacctcga cagcacggtc tacagcgtct tcctgatcct
      421 tcaggtcggg ctcgatccgc tccaactcgt gctgatgggc acgatcctcg aggtctcgta
      481 tctcctgttc gaggtcccga ccgggatcgt cgccgacacg gtcagccgga agtggtcgat
      541 cgtgatcggg tacgtcggga cgggcatcgc gttcgtgatg ctgggatctg ccgactcctt
      601 cggggccgca gccctgtcgc aggtcctcta cggcgtgtcg gcgacgttcg tgagcggcgc
      661 ggacgtggcc tggctcaccg atgaagtggg tgaggacgcg gcgcgaccgc tctacgtccg
      721 gagcgagcag ttcttcaacg gcggggcgct ggtcgggatc gccggatgcg tggcgctcgc
      781 gtcgatcgcg ctccggctcc cgatcctcgt ctgcggtctc ggatacatcg cgctaggcct
      841 gtcgctcgcg gtcgggatga cggagacccg gcgccccacc cgtgacgccg gggcgaagct
      901 ccgacactcg atgcacgtca cgatgcggga tgcgatccaa caggtccgtg cacatcacat
      961 cctgctcctg atcctggcga cggctgtgct gcacggggcc tcgaccgagg gctgggatcg
     1021 actatccgat ctccagctcc tcagtggcat cggactgccc tcgctcggcg acctcgagcc
     1081 gatcgtgtgg ttcgggatcc tcgacggtgt gggactggta ctcggcatcg ccgcgctctc
     1141 ctacgtgcgg cgccgggggc atctggaggg ccacggacac gtggcgaagc tcctcgctct
     1201 cgtcgacgta tcgctgatcg tcagcgtcgt gtggttcgcg ttcgccggaa gcttctggtg
     1261 ggcggcgatc ctcttctgga tcgtcggcgg cctccggagc gtgcgcggtc cggtgttcac
     1321 ggcctggatc aaccaggggc tcgaccccgc gacccgggcc acgatcaact cgatgggagg
     1381 ccaggccgat gccgtcggtc aggcgatggc cgggccggtg gtcggagggg tcggccgtgc
     1441 cgtctcggtg ccgtgggccc tcacgctcgc cggcctgctg cgactgccga tcctgctcct
     1501 ctacctccgt gcgatcaggc gcggcaccgt cggcacagtc gcgcccgacg cgatggatca
     1561 ggagatcgat ctcgccgact gacgctccgg agggggagga gggagcgcgg cgacgtcgcc
     1621 gtgatggtgg tgcgtctccg gtgcgcgtcg gcgaccgcgg tcgcggatcc cgcactcgac
     1681 gcggagtacg ccggccgtgc gccgagcgat cgcgtacgcg aggtcgtgga cgtccgacgg
     1741 gaggttcccg ccgaggaacg cggtccgagc gtgcacccga acggtgagcg atcgggcgcc
     1801 acgggtgcgc ggctcccgcg cgagcgcggt acggagcctc gcctcggcga cgaggtcgcc
     1861 ccggtcagcg gccgacggcg gcacagccct ccacgcgaat ccgagccacc gtcccacttc
     1921 cctccgattc cgccacgccc accacgcggc ggtcaggcga cccacgggcg agaatctcca
     1981 caaccacgag atcactcgga acacgtcctt tccagtccgt ctgggatgtc tctacccgcg
     2041 atgcccatgc ggcgaacgtt tctcctcggc acgtccgggg tagcggcgat gtgagcgtcg
     2101 tcgaccgacg gcgctcatcc ccccaaggag gtggatgttc gtgatcgaga tgtggacgta
     2161 ccgcacagat gtggtgttgc agccagccgt ggatctcgtc ggcttcggca tcgaggctcg
     2221 cgacggcagc atcggaaagg tcgacgagtc gaccgccgat gccgggaaga gctacgttgt
     2281 cgtggacacg ggtccctgga tcttcggccg caaggtgctt ctgccggcgc agaccgtgga
     2341 gcggatcgac ctcgacgctc ggaccgtctt cgtcgaccgg acgaaggagg agatcaagaa
     2401 cgctcccgag tacgacccgg agcgtgggat cgaccccacg tatcaggacg agctcggcgg
     2461 gtactacggt cggtactact cgtaaccgcg gagagctcgg cggacggacc cggagggcga
     2521 tcgccctccg ggtcctccct catccgcgat cggccttcgt cgcccagagc atcacgtcat
     2581 ccgcgtcgtc
//
LOCUS       DASUVE010000034         3396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_19603, whole genome shotgun sequence.
ACCESSION   DASUVE010000034 DASUVE010000000
VERSION     DASUVE010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19603"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..2041)
                     /locus_tag="VF036_00685"
     CDS             complement(302..2041)
                     /locus_tag="VF036_00685"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEX6263774.1"
                     /translation="MSSTAERAPAAEHTWVEALRTGTTPVPVPFRGHELLEHALLNKD
                     TAFSEHEREAFGVRGLLPPRVRTLEEQVALEIAKNRRKDDELERFIGLAALQDRNETL
                     FHRLLLDNLEELMPIVYTPVVGRACQEYSHIMRRPRGLWLTPEDVDRIPELLSNARHE
                     DVRLIVATDNERILGLGDQGAGGMGIPVGKLALYTAGAGIHPALTLPVSLDVGTDNEA
                     LLNDPGYFGHREPRLRGQAYDGFIEAFVEGVLEVFPHAVLQWEDFKQHNAIRLLDRYR
                     HRITSFNDDIQGTAAVVMAGIRSALRILDEPLRAQRFAFLGAGAAGIGIARLVRAQLL
                     REGVSEGDARRAIVQLDSRGLTYVGREPLDGDKEAFALGADEMGTFGFVAGETYGLEE
                     VVEHVHPTFLIGTCGTPGAFTEAAITEMAAHARLPVILALSNPTSRTEAQPRDILGWT
                     RGRAIVATGSPFPPVVRGARTHVIGQANNAFCFPGIGLGALVAEAREVTDEMFLVAAE
                     ALAGVVPEARLAGGSLYPKPSDLRIASRAIALAVARAARDAGVGRQLRDDELGSAVDA
                     MMWEPRYLPYEPA"
     gene            complement(2038..>3396)
                     /locus_tag="VF036_00690"
     CDS             complement(2038..>3396)
                     /locus_tag="VF036_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX6263775.1"
                     /translation="FAAACFVLALKWLSHPTTARRGVRTGEIGMLVAVVATLADPRIV
                     EYRWLGVAIVAGVALGGPLGLLVPMTAVPQRTALSHAFGALAAGLVGTAEYYLRRPEI
                     DGTTMVAIGLEVVLGFLTVTGSLIAAGKLQGTLPDRPWMYRGQNVVNVSVLCVALVLV
                     GILVIDPGRAVLFPIVIVVSLVFGSLLVIRIGGADMPTVISLLNGFAGLSASMMGFVL
                     DNNLLIVAGALDGSSGLVLSITMCKAMNRSFANVLFGGFGAATSSGDVERRPFRSIDA
                     EEAAFLLDAARSVVVVPGYGMAAAQAQHKVRELSDALQRRGVDVKFAIHPVAGRMPGH
                     MNVLLAEADVPYDQLFDLEQINSEFPQTDVVLIVGANDVVNPAARTDTTSPIYGMPIL
                     EVEAASSIIVVKRSMRSGFAGLDNDLFYLDKTQMLFGDAKAAIGQVLDELSKLPAKEA
                     AG"
BASE COUNT          577 a         1159 c         1136 g          524 t
ORIGIN      
        1 cgagggtcac ggcagcgatg cccgacgcat ccaccgtgag aggcgagccg ctccgggggg
       61 cggccgatcg cgtggacgat ctcaacgcga tcagcgctgc gagcgcagcg gtcagaagga
      121 cgaccccgat caggatccag aggatgatca ccgtatctct ctcctccgct tcgtcaccgc
      181 gatgctacgc gcagggacct cttccgggag gagtcgttgg tccgtgaacc gtggggtcga
      241 acgtcccatg ttgaccactg ttgcccggat ccaggtccct gcaccctctc cgcgtgcgcc
      301 cctaggcggg ttcgtagggc aggtaccgcg gttcccacat catcgcgtcc accgccgatc
      361 cgagctcgtc gtcgcggagc tgtcggccga cccccgcgtc ccgtgccgca cgtgccaccg
      421 cgagggcgat cgcccgagag gcgatccgca gatccgatgg tttcggatag agcgaacctc
      481 ccgcgaggcg ggcctcggga accacgcccg cgagcgcctc ggccgctacg aggaacatct
      541 catcggtgac ttcgcgcgcc tccgcgacga gggccccgag cccgatcccc gggaagcaga
      601 aggcgttgtt cgcctgaccg atcacgtgcg tccgcgcgcc acggacgacg ggtgggaacg
      661 ggctgcccgt cgcgacgatc gctcgacctc gggtccatcc caggatgtcc cgaggttgcg
      721 cctccgtcct cgaagtcgga ttcgacaacg cgaggatcac cgggagccga gcgtgggcag
      781 ccatctcggt gatggcggcc tcggtgaatg ctccgggagt tccgcatgtt ccgatcagga
      841 atgtcgggtg gacgtgctcc acgacctcct cgagaccata cgtctcgccg gcgacgaacc
      901 cgaatgtccc catctcgtcc gcgccgagcg cgaatgcctc cttgtcgcca tcgagcggtt
      961 cgcgcccgac atacgtcaac cctcgcgagt ccagctggac gatggcgcgc cgggcatccc
     1021 cctccgagac gccttcgcgg agcaactgcg cccgcacgag acgggcgatc ccgatcccag
     1081 cggcgcccgc cccgaggaac gcgaagcgct gggcacggag cggctcatcg aggatccgca
     1141 gggctgagcg gatcccggcc atgacgaccg cggcggtgcc ctggatgtcg tcgttgaagc
     1201 tcgtgatccg atgccggtag cggtcgagca ggcggatcgc attgtgctgc ttgaagtcct
     1261 cccactggag cacggcatgc gggaagacct cgaggacccc ttcgacgaat gcctcgatga
     1321 agccgtcgta cgcctggccc cgcaggcgag gttctcggtg tccgaagtag ccggggtcgt
     1381 tcagcagcgc ttcgttgtcg gtccccacgt ccagggacac cggcagggtg agggccgggt
     1441 ggatccccgc tccggctgtg tagagagcga gcttgccgac cgggatcccc atgccacccg
     1501 ccccctggtc gccgagaccg aggatccgct cattgtccgt cgcgacgatg agacgaacgt
     1561 cttcgtgccg ggcgttcgag agaagctcgg ggatccgatc gacgtcctcg ggtgtgagcc
     1621 acaggccgcg cggtctccgc atgatgtggc tgtactcctg gcaggcgcgg ccgacgaccg
     1681 gtgtgtagac gatcggcatc agctcctcga ggttgtcgag caagagccgg tggaacaagg
     1741 tctcgttccg gtcctggaga gccgcgagcc cgatgaaccg ctcgagttcg tcgtccttgc
     1801 ggcggttctt ggcgatctcg agagccacct gctcctcgag cgtccggacg cgtgggggca
     1861 acagcccacg gacgccgaag gcttcccgct cgtgctcgct gaacgcggtg tccttgttga
     1921 ggagcgcgtg ctcgagaagc tcgtgtccgc ggaagggaac gggcaccggc gtggtgcccg
     1981 ttcgcagcgc ctcgacccag gtgtgctcgg cagccggtgc tcgttccgcg gtcgagctca
     2041 tcccgccgcc tccttcgcgg gaagcttgga gagctcgtcg aggacctgtc cgatcgccgc
     2101 cttcgcgtcg ccgaagagca tctgggtctt gtcgaggtag aacaggtcgt tgtccaggcc
     2161 ggcgaaccct gagcgcatcg agcgcttgac gacgatgatc gaagatgcgg cctcgacctc
     2221 gaggatcggc atcccgtaga tcgggctcgt cgtgtcggtg cgcgccgcag ggttcacgac
     2281 atcgttcgct ccgacgatga gcacgacgtc ggtctgcggg aactcgctgt tgatctgctc
     2341 gaggtcgaac agttggtcgt agggcacgtc cgcttccgcg aggaggacgt tcatgtgacc
     2401 gggcatccgg ccggcgaccg ggtggatcgc gaacttcacg tcgacaccgc ggcgctgcaa
     2461 cgcatcggag agctcgcgca ccttgtgctg tgcctgggcc gccgccatgc cgtagccggg
     2521 gacgaccacg accgatcgcg cggcgtcgag gaggaacgcc gcctcctcgg catcgatgct
     2581 acggaacggc ctccgctcga catccccgct cgaggtcgcg gcaccgaatc ccccgaacag
     2641 cacgttcgcg aacgaacgat tcatcgcctt gcacatggtg atcgacaaga ccaacccact
     2701 cgacccgtcc aacgcgccgg ccacgatcag caggttgttg tcgagaacga aacccatcat
     2761 cgaggccgag aggcccgcga agccgttcaa gagggagatc acggtcggca tgtccgcccc
     2821 gccgatccgt atcaccagca acgagccgaa cacgagcgag accacgatga cgatcgggaa
     2881 gagcaccgct ctgccggggt cgatcaccag gatcccgacc aggacgagag ccacgcagag
     2941 gacggacacg ttgacgacgt tctgacctcg gtacatccag ggccgatcgg gaagggttcc
     3001 ctgaagcttg ccggccgcga tcaacgaccc ggtcaccgtc aagaagccca ggacgacctc
     3061 gagcccgatt gcgaccatgg tcgtgccgtc gatctccggc cgccggaggt agtactcggc
     3121 agtcccgacg aggccggccg ccagcgcgcc gaaggcatgg gacaacgccg tcctctgggg
     3181 gaccgccgtc atcgggacca gcaggccgag ggggcctccg agcgccacgc ccgcgacgat
     3241 cgccacgccg agccatcggt actcaacgat ccggggatcg gcgagcgtcg cgacaacggc
     3301 gacgagcatg ccgatctcgc cggttcgcac gccgcgccgc gcggtcgtgg gatgcgagag
     3361 ccacttcaac gcgaggacga agcaggccgc cgcgaa
//
LOCUS       DASUVE010000035         3296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_961328, whole genome shotgun sequence.
ACCESSION   DASUVE010000035 DASUVE010000000
VERSION     DASUVE010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961328"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1220)
                     /locus_tag="VF036_00695"
     CDS             complement(87..1220)
                     /locus_tag="VF036_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6263776.1"
                     /translation="MARGEIRLIGLSKRFADVTAVDHLDLVIADGEFFSLLGPSGCGK
                     TTTLRMIAGFEQPSDGRILLDGVDVAYTPPHKRNVNTVFQNYALFPHLNVFDNVAFGL
                     RRAKCPKDELKRRVAEALELVRMSGLERRKSSQLSGGQQQRVALARALVLNPAVLLLD
                     EPLGALDAKLRKALQIELKTLQQEVGITFLYVTHDQEEALTMSDRLAVMNGGLIEQIG
                     APQEVYEDPETLFVADVLGVSNLMDATGRGPVNGRCRVEVGRFELEAGGGPSDVTGPA
                     KLVIRPERVELERHEADSGPNCLPGMVERVVYVGSAVQVLVRVATGETLQALVTNTGK
                     ELPYEQGTPVQLHLPVEALRVLPVAGAEEPVDDPRVSSGLESR"
     gene            complement(1236..2135)
                     /locus_tag="VF036_00700"
     CDS             complement(1236..2135)
                     /locus_tag="VF036_00700"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6263777.1"
                     /translation="MSTAAEVQPPPRLSAWSRFHGWLTNPWGRPRFLVLFTWAYMVWA
                     LVPVLIAVQFSFNDSRSRTVWQGFSTRWYWGDPVDSVWHDPTLRNALAQSLKLATADV
                     LIATPIGVLLAIGLARWTGWGSRPSNFLMLFPLVTPEIVMGVSLLLLFTHVFVFVGTG
                     TLAQILGHVTFSISYVVVIVRGRLFSIGRQYEEAAADLGASPTVALYRVLLPLLMPAI
                     IASAAVVFAISLDDFVISQWLSKGGETDTVPIQIYAATRAAPLPSTNAIATVMMVLTL
                     VSVTAGYLVWRAFTRGERRTEDR"
     gene            complement(2132..3025)
                     /locus_tag="VF036_00705"
     CDS             complement(2132..3025)
                     /locus_tag="VF036_00705"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6263778.1"
                     /translation="MYRRWLWPGFALPGIVWLIVLFLVPFYAVVATAFGTVDPVLGQA
                     VPFWNPLEWNVGWIQEVLEQLAPGGRFFGAAVRTIVYVGIALGLSLLIGYPVAYYIAR
                     HAGRTKSLLLILLIAPLWINYLMRMLAWVNLLAPDGYVNRFLTFTHVLSEPRDWLGGR
                     ASTVILALVYGYIPFMILPLFAALDRIDRSHLEAARDLGANRFSTFLHVTLPLSKAGI
                     LGGAVLIALPMFGDYYTPNIVSGAPTTSMIGNQIDSYFHVGGQPTIGAAITVVLSVFL
                     TFLMAYYLWTIHKASREARIA"
     gene            complement(3035..>3296)
                     /locus_tag="VF036_00710"
     CDS             complement(3035..>3296)
                     /locus_tag="VF036_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263779.1"
                     /translation="LSHLWLNYIIDNGVAYSNFVDFNGYQPPLNEIDPSSLVKDGVVP
                     EHLANSVLTNDDLGPDSLQYATLTSRGQQLWQDGYSDFTAGG"
BASE COUNT          605 a         1039 c         1165 g          487 t
ORIGIN      
        1 ttgtccgcat cacgaccggg ccgatgccgc tgatggagcg agtggcgcgc gcggtggccg
       61 aagtgcgagg agagctgctc gagtcctcag cggctctcga ggcctgacga gactcgcggg
      121 tcgtcgaccg gctcttctgc gccggcgacc ggcaggacgc gcaacgcctc gacgggcagg
      181 tgcagctgca ccggggtccc ttgctcgtac gggagctctt tgccggtgtt cgtcaccagc
      241 gcctggagag tctcgccggt agcgacgcga acgagcacct gaacggccga gccgacgtac
      301 accactcgct cgaccattcc cgggaggcag ttcggacccg agtcggcctc gtggcgttcg
      361 agctcgaccc gctccggccg gatcacgagc ttcgcggggc cggtcacgtc gctcgggccg
      421 ccgcccgcct cgagctcgaa ccgtcctacc tcgacccggc agcgcccgtt gaccgggcca
      481 cggccggtgg cgtccatcag attggacacg cccaggacgt ccgcaacgaa gagtgtctcc
      541 gggtcctcgt agacctcctg tggcgcgccg atctgctcga tcagaccgcc gttcatgacg
      601 gccagacggt cggacatcgt cagagcctct tcctggtcgt gcgtcacgta gaggaacgtg
      661 atcccgacct cctgctgcag cgtcttcagc tcgatctgca gcgctttccg cagcttcgcg
      721 tcgagcgctc ccaacggctc gtcgaggagg aggaccgcgg ggttcaggac gagcgcgcgg
      781 gcgagcgcga cccgctgctg ctggccgccc gagagctgag aggacttccg gcgctcgagg
      841 ccgctcatgc ggacgagctc gagcgcctct gcgacccgcc gcttcaactc gtccttcggg
      901 cacttcgcgc gccggagccc gaaggcgacg ttgtcgaaca cgttcaggtg cgggaagagc
      961 gcgtagttct ggaacaccgt gttgacgttc cgcttgtgcg gaggggtgta cgccacgtcg
     1021 actccgtcga gaaggatgcg gccatccgag ggctgctcga accccgcgat catccgcagc
     1081 gtggtcgtct tcccgcagcc ggacggcccg agcagcgaaa agaactcgcc gtcggcgatc
     1141 acgaggtcga ggtgatccac cgccgtgacg tcggcgaaac gcttgctcaa cccgatcagc
     1201 cgtatctctc cgcgggccat gacgccttct ggaacctacc tgtcctccgt gcgccgctcg
     1261 ccgcgggtga acgcacgcca gacgagatag ccggccgtga ccgagacgag cgtgaggacc
     1321 atcatcaccg tcgcgatggc gttcgtggag gggagcggag ccgcgcgtgt ggcggcatag
     1381 atctgaatgg ggacggtgtc ggtctcacca cccttcgaga gccactggct gatgacgaag
     1441 tcgtcgagtg agatggcgaa gacgaccgcc gccgaggcga tgatcgccgg catcaggagc
     1501 ggcaggagga cgcggtagag cgcgaccgtg ggggaggcgc cgagatcggc ggccgcctcc
     1561 tcgtactgtc ttccgatcga gaacaggcgg cccctgacga tgacgacgac gtaggagatc
     1621 gagaaggtca catggccgag gatctgcgcc agcgtcccgg tcccgacgaa cacgaacacg
     1681 tgggtgaaga gaaggagcaa agagactccc atgacgatct ccggcgtcac gagcgggaag
     1741 agcatgagga agttcgaggg cctcgacccc cagccggtcc accgggccag cccgatcgcg
     1801 agcaggactc cgatcggcgt tgcgatgagg acgtcggcgg tggcgagctt caggctctgg
     1861 gccagagcgt tccgcagcgt cgggtcgtgc cacacggagt cgaccgggtc tccccagtac
     1921 cagcgggtcg agaagccctg ccacacagtg cgcgaccgcg agtcgttgaa cgagaactgc
     1981 accgcgatca ggaccgggac gagggcccag accatgtagg cccaggtgaa gagaacgagg
     2041 aagcgagggc gtccccaggg gttcgtcagc cagccgtgaa accggctcca ggcggagaga
     2101 cggggcggcg gctgaacctc ggcggccgtg ctcatgcaat cctcgcctcc cgggaggcct
     2161 tgtggatcgt ccacaggtag taggccatga ggaacgtgag gaacacggag agcacgacgg
     2221 tgatcgcggc cccgatcgtc ggctggcccc cgacgtggaa gtaggagtcg atctggttgc
     2281 cgatcatcga cgtggtcggc gcgcccgaga cgatgttggg cgtgtagtag tcgccgaaca
     2341 tcggcagagc gatgaggacc gcgccgccga gaatgccggc cttcgacagc ggcagagtga
     2401 cgtggagaaa ggtgctgaac cggttcgccc cgaggtcccg cgccgcctcg aggtggctcc
     2461 ggtcgatccg gtcgagtgcg gcgaagaggg gcaggatcat gaacgggatg tagccgtata
     2521 ccaacgccag aatcacggtc gacgcccgtc ctccgagcca gtcgcgcggc tccgagagca
     2581 cgtgcgtgaa cgtgagaaac cggttcacgt acccgtccgg agccaggagg ttgacccacg
     2641 caagcatccg catcaggtag ttgatccaga gaggagcgat gaggaggatc aggagcaggc
     2701 tcttcgtccg accggcatgg cgggcgatgt agtaggcgac gggatagccg atcaggagcg
     2761 agaggccgag cgcgatgccg acgtagacga tggttcgtac ggcggccccg aagaacctac
     2821 ccccgggcgc cagttgctcg agaacctcct ggatccagcc gacgttccac tcgagcgggt
     2881 tccagaaggg gacagcctgc ccgaggacgg gatcgacggt gccgaacgcg gtggccacga
     2941 ccgcatagaa ggggacgagg aagaggacga tcagccagac gatgcccggg agggcgaacc
     3001 cgggccagag ccagcggcgg tacacggagc tcctttagcc cccggctgtg aagtccgagt
     3061 agccgtcctg ccagagctgc tggccccgcg acgtcagcgt cgcgtactgc agcgagtcgg
     3121 gcccgaggtc gtcgttcgtc agcacgctgt tggccaggtg ctccgggacg acgccatcct
     3181 tcacgagcga ggacgggtcg atctcgttca gcggcggctg gtagccgttg aagtcgacga
     3241 agttcgagta ggcgacgccg ttgtcgatga tgtagttcag ccagagatga gagagg
//
LOCUS       DASUVE010000036         3574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_585682, whole genome shotgun sequence.
ACCESSION   DASUVE010000036 DASUVE010000000
VERSION     DASUVE010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585682"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VF036_00715"
     CDS             <1..352
                     /locus_tag="VF036_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX6263780.1"
                     /translation="GAPALAPGVAVGKGIVIEAILTFFLVFVVFGTAVDDKGPWNKTA
                     GFTIGFTIAFDILAFGPLTGAAMNPARWFGPALVGGIWENAVVWIVGPISGAIIAGVL
                     YTAVLLRGREPATP"
     gene            384..1568
                     /locus_tag="VF036_00720"
     CDS             384..1568
                     /locus_tag="VF036_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX6263781.1"
                     /translation="MVADLYLDSASAAPLLPEARDAVVAALESFGDPLSIHAPGRAAR
                     SLLDDGREQVATAIGAQPDEIVFTSGGTESIALAIWGGVRAVRELGTRIVIGAVEHPA
                     VSGVAHALESDGFEVVVVPVDRDGRVDLDRFAADVRAPGTLLASLQHANHELGTIQPV
                     AEAARLARGAGVRFHTDACQTVGRLPVDGPTLGADLLSLSAHKFGGPPGVGALYVRRG
                     VAITAYPCGDDRERKRRSGMENTPGIAGMAAALTASLTTMADRAASHWTLTAELRERL
                     AEVPGTTVHGHPTHRTPHLVGFSVAGLDPATLLMTLDDRGYRLGAGSLCSGRPEDPSS
                     VLEQIGYPVTTGFRVSLGDGVDRSHLDALVGMLSGTVRELRQMEAASTEALSRFSPPT
                     AG"
     gene            complement(1597..2640)
                     /locus_tag="VF036_00725"
     CDS             complement(1597..2640)
                     /locus_tag="VF036_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014054094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX6263782.1"
                     /translation="MSDVIANGGTAPRDASTLTKKDFTSDQEPRWCPGCGDYAILSAV
                     QAFMPELGIEPHKTVFVSGIGCSGRFTYYMDTYGMHGIHGRAPALATGIAVAAPELSV
                     WVVTGDGDGLSIGGNHLIHALRRNVDIKILLFNNQIYGLTKGQYSPTSEEGKVTKSTP
                     FGSVDHPFNPIALALGVEATFVARTVDNDRQHLTEVLRRAAQHKGTAFVEIYQNCNVF
                     NDGAFDLLRDKAQGKHNQIRLEHGEPIVFDEGNRCVRVGENGRYEIALTAETEPDGIV
                     VHDAHGRPSLAFALAHLSRGPDEPSCIGVFHEVERPVYGQAIQHQLQAAKERLGAGDL
                     GSLLHVGDTWTVE"
     gene            complement(2633..>3574)
                     /locus_tag="VF036_00730"
     CDS             complement(2633..>3574)
                     /locus_tag="VF036_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007823235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEX6263783.1"
                     /translation="GAAFSGQLAVTGTSGPGIDLKAETIGLAVIMELPMIVVDVQRAG
                     PSTGMPTKTEQADLLLAMYGRHGESPLPIVSASTPAECFTAALEAARIAVRYRTPVIL
                     LSDTFIANSSEPWAIPDVESLPTIDPNFATPNGPDFLPYSRDERLARPWATPGTPGLV
                     HRIGGLERQDGTGNISYDAANHELMTRLRQAKVDGIAEDIPALEVEDPSGEAELLVLG
                     WGSSLGTITAAVARARADGRSVATAHLRHLNPFPTNTGEVVRAYRKVLIPEMNMGQLA
                     ALIRARYLVDAASYTKVQGLPIFAEELEHEITRVLDE"
BASE COUNT          488 a         1280 c         1228 g          578 t
ORIGIN      
        1 gggcgccccg gcgctcgcac cgggcgtcgc cgtcggcaag ggcatcgtca tcgaggcgat
       61 cctcacgttc ttcctggtct tcgtggtgtt cggaaccgcc gtcgacgaca agggtccgtg
      121 gaacaagacg gcggggttca cgatcgggtt cacgatcgcc ttcgacatcc tcgcgttcgg
      181 accgctgacc ggcgcggcga tgaatcccgc gcgctggttc ggcccggcgc tggtgggtgg
      241 gatctgggag aacgcggtcg tctggatcgt gggaccgatc tctggcgcca tcatcgccgg
      301 cgtcctctac acggccgtgc tcctgcgcgg acgggagccg gccaccccgt agctccgggc
      361 ccgcccggta cgctgggccg gcgatggtcg ccgaccttta tctcgactcg gcctcggccg
      421 ctcccctgct tccggaggcg cgcgacgccg tcgtcgccgc cctcgagtcg ttcggcgacc
      481 ctctctcgat ccacgccccg ggacgtgcgg cccgctctct gctcgacgac ggacgcgagc
      541 aggtcgccac cgcgatcgga gcccagcccg acgagatcgt gttcacctct ggcggcaccg
      601 aatcgatcgc actcgcgatc tggggcggcg tacgcgctgt ccgagagctc ggcacccgga
      661 tcgtgatcgg tgccgtcgag catcccgccg tgagcggtgt cgcgcacgcg ctggagtcgg
      721 atgggttcga ggtcgtggtg gtccccgtcg accgagacgg tcgggtcgat ctcgatcgct
      781 tcgccgcgga cgtgcgagct cccggcacgc tcctcgcatc gctccagcac gcgaaccacg
      841 agctcggaac gatccagccc gtcgccgagg cggcccgact cgcgcgcgga gccggcgtgc
      901 ggttccacac cgacgcctgc cagaccgtgg gccggcttcc ggtggacgga cccaccctcg
      961 gcgcggacct cctgtcgctg tcggcgcaca agttcggagg gccaccgggg gtgggggccc
     1021 tctacgtccg tcgcggcgtc gcgatcacgg cctacccctg cggggacgac cgagagcgga
     1081 agcgtcgctc cgggatggag aacacccccg ggatcgccgg gatggcggcc gcgctcacgg
     1141 cgtccctcac cacgatggcc gaccgagccg cctcccactg gacgctgacc gcggagctcc
     1201 gggaacggct ggccgaggtg cccggcacga cggtccacgg tcacccgacc caccgcacgc
     1261 ctcacctggt ggggttcagc gtcgcaggcc tcgaccccgc gacactgctg atgaccctcg
     1321 acgatcgtgg ctaccgactg ggagccggat cactctgcag cggccgcccc gaagacccgt
     1381 cgtcggtgct ggagcagatc ggctacccgg tgacgacggg attccgggtg agtctcgggg
     1441 acggtgtgga ccgatcccac ctcgatgctc tcgtcgggat gctctccggg accgtgcgcg
     1501 aactccgaca gatggaggcg gcctccacgg aggcactctc gcggttcagt ccgccgaccg
     1561 ccggctgagc ccagtcccgc ggaggcccgc cggccgctac tccaccgtcc aggtgtcccc
     1621 gacgtggagg agcgagccga ggtcgcccgc gccgaggcgc tccttcgcgg cttggagctg
     1681 atgctggatc gcctgcccgt acaccgggcg ctccacctcg tggaagacac cgatgcacga
     1741 gggttcatcc gggccgcggg agaggtgggc cagcgcgaac gcgagcgacg gccggccgtg
     1801 ggcgtcgtgc acgacgatgc cgtccggttc ggtctccgcc gtgagcgcga tctcgtaccg
     1861 gccgttctcg ccgacgcgca cgcaccggtt cccctcgtcg aacacgatcg gctcgccgtg
     1921 ctcgagccgg atctgattgt gcttgccctg ggccttgtcg cgcagcaggt cgaacgcgcc
     1981 gtcgttgaag acgttgcagt tctggtagat ctcgacgaag gccgtcccct tgtgctgggc
     2041 ggcccgccgg aggacctcgg tcagatgctg ccggtcgttg tcgaccgtgc gtgccacgaa
     2101 cgtcgcctcg accccgaggg cgagtgcgat cgggttgaac ggatggtcga cggagccgaa
     2161 cggcgtggac ttcgtgacct tcccctcttc cgacgtgggc gagtactggc ccttcgtcag
     2221 tccgtagatc tggttgttga agaggaggat cttgatgtcg acgttccggc ggagcgcgtg
     2281 gatcaggtgg ttccccccga tcgagaggcc gtcgccgtcg ccggtcacga cccacaccga
     2341 gagctccgga gccgcgaccg cgatcccggt ggcgagggcg ggggcacgcc cgtggatccc
     2401 gtgcatgccg tacgtgtcca tgtagtacgt gaaccgcccg ctgcagccga tcccggacac
     2461 gaacaccgtc ttgtgcggct cgatgccgag ctcaggcatg aacgcctgga ccgcggacag
     2521 gatcgcgtag tcgccgcacc cggggcacca gcgcggctcc tggtccgatg tgaagtcctt
     2581 cttggtcagc gtcgaggcgt cacgcggtgc ggttccgccg ttcgcgatca cgtcactcat
     2641 cgagcaccct cgtgatctcg tgctcgagct cctccgcgaa gatcgggaga ccctgcacct
     2701 tcgtgtacga cgccgcgtcc actaggtacc tggcacggat cagcgctgcg agctgcccca
     2761 tgttcatctc cgggatcaac accttccggt acgcgcgcac gacctccccg gtgttcgtgg
     2821 ggaacgggtt cagatgccgg agatgcgcgg tcgccacgct ccggccgtcg gccctcgccc
     2881 gggcgacggc agccgtgatc gtgccgagcg aggagcccca gccgagcacg agcagctcgg
     2941 cctcaccgct cgggtcctcg acctcgaggg ccgggatgtc ctccgcgatg ccgtcgacct
     3001 tcgcctggcg gagcctggtc atcagctcgt ggttggccgc gtcgtagctg atgttgccgg
     3061 tcccgtcctg gcgttccaat ccaccgatcc ggtgcaccag ccccggtgtc cccggggtcg
     3121 cccacggccg cgccaacctc tcatcgcgcg agtacggcag gaagtcgggg ccgttcggcg
     3181 tggcgaagtt cggatcgatc gtcgggaggc tctcgacgtc ggggatcgcc cacggctccg
     3241 acgagttcgc gatgaacgtg tccgacagca ggatcaccgg cgtccggtac cgcacggcga
     3301 tccgggccgc ctcgagcgca gcggtgaagc actcggccgg ggtggacgcc gacacgatcg
     3361 gcagcggcga ttccccgtgc cgtccgtaca tcgcgagcag caggtccgct tgctcggtct
     3421 tcgtcggcat gccggtcgag gggcccgcac gctgcacgtc gacgacgatc atcggcagct
     3481 ccatgatcac cgcgagcccg atcgtctcgg ccttcaggtc gatgcccggc ccgctcgttc
     3541 cggtcacggc cagctggccg ctgaacgccg cgcc
//
LOCUS       DASUVE010000037         2194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_586977, whole genome shotgun sequence.
ACCESSION   DASUVE010000037 DASUVE010000000
VERSION     DASUVE010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586977"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VF036_00735"
     CDS             <1..183
                     /locus_tag="VF036_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX6263784.1"
                     /translation="PLLDGVRGAPPSDVDALAHAVSRLSVLATELGDRIAELDVNPVI
                     VSPSGCVAVDALVVPA"
     gene            349..495
                     /locus_tag="VF036_00740"
     CDS             349..495
                     /locus_tag="VF036_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016826775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263785.1"
                     /translation="MKRERIEKPKPRRPRELEMALPLDPRDPDVVRAKKGWVWRYADR
                     RWAV"
     gene            complement(500..1909)
                     /locus_tag="VF036_00745"
     CDS             complement(500..1909)
                     /locus_tag="VF036_00745"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6263786.1"
                     /translation="MDIGPYGETSDERHPMPLSRRRFLQTSAMAVAGGVLFSCTGGTR
                     IPQVDDTIPETDTRWPIKRVIYVMLENRSFNNLFGKFPGVRAGTTVGVENGVEKPLIR
                     CPEWLPGDIPHDLAAHRTCVNNGEMDGFGTGIHGSTYGYTIFDEDQIPNYWHWAREYG
                     LSDSFFASAAGPSYPNHYYFIAGSSGGVADNPENIRTREMENGNTFKSWGCDVVGDDV
                     FVLVTDEAGNLTKHNTCFEFPTVGEQLTQAGIDWAYYSAVPGQPGYFWNAYNGIANVF
                     HTDLWPEHIRSVDTLLDDIDAGALPPVTWVTPRFQLSDHPPFSTGHAHNWVTKIVNKV
                     MRSDMWEHTAIFLTWDEWGGFYDPVVPPSVDHLGLGIRVPLLTISPYTRRGVIDDERG
                     EFSTPLRFISDNWGLDPLTDRIRNTHNLEHVFDFAGRPRAPELGREQAPTFSPSPWVF
                     PRDTYTGWEPGTVPVEDPL"
     gene            1965..>2194
                     /locus_tag="VF036_00750"
     CDS             1965..>2194
                     /locus_tag="VF036_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263787.1"
                     /translation="MRKHMIAAALLLFLVACDGGPEPFPPPSSPTSASPFPSTPTLAS
                     PTPPALPSNPLPKSGDASETCVEGWVSPGVESA"
BASE COUNT          330 a          733 c          747 g          384 t
ORIGIN      
        1 ccactcctcg acggcgtgcg cggggcgccg ccgtccgacg tcgacgcgct cgcccacgcc
       61 gtctcccgcc tgtcggtgct cgccaccgag ctcggtgatc ggatcgccga gctcgacgtc
      121 aacccggtga tcgtgtcgcc atcgggctgc gtcgccgtcg acgcgctcgt cgtccccgca
      181 taggcggccg gagaaggtca cacccgccgt gtgacgctcg acatggttct tcctccgagg
      241 gagggcgtac aaccgatatc gagggggtat ggcctcagac atgatccgcc gacggatcgg
      301 acgcgaacct ctcgggacgg aacaggcgga gcgcaggagg tgacgggcat gaagcgcgaa
      361 cggatcgaga agccgaagcc ccgccgcccg cgcgagctcg agatggccct cccgctcgat
      421 ccccgcgatc ccgacgtcgt tcgcgccaag aagggctggg tctggcggta cgcggatcgc
      481 cgctgggccg tctagcgggc tagagcgggt cctccaccgg gaccgtcccc ggttcccatc
      541 ccgtgtacgt atcgcggggg aacacccacg gtgagggcga gaacgtcggc gcctgctccc
      601 tgccgagctc cggcgcccgc ggtcggccgg cgaagtcgaa gacatgctcg aggttgtggg
      661 tgttgcggat ccggtcggtg agcggatcca gcccccagtt gtccgagatg aaccgcaagg
      721 gcgtcgagaa ctcgcctcgc tcgtcgtcga tcacgccccg tcgcgtgtac ggcgagatcg
      781 tgagcaacgg gacgcggatt cccaggccga ggtgatcgac ggagggcgga acgacgggat
      841 cgtagaagcc gccccactcg tcccacgtca ggaagatggc cgtgtgctcc cacatgtcgg
      901 agcgcatcac cttgttcacg atcttcgtga cccagttgtg ggcgtggccc gtggagaacg
      961 ggggatggtc ggagagctgg aacctcgggg tcacccaggt gaccggcgga agcgcgccgg
     1021 cgtcgatgtc gtcgaggagc gtgtcgaccg atcggatgtg ctcgggccac aggtccgtgt
     1081 ggaacacgtt cgcgatgccg ttgtacgcgt tccagaagta gccgggctgt cccgggaccg
     1141 ccgaatagta ggcccagtcg atgccggcct gggtgagctg ctcgccgacc gtggggaact
     1201 cgaagcaggt gttgtgcttc gtcaggttcc ctgcctcgtc cgtcaccagg acgaacacgt
     1261 cgtctcccac gacgtcgcag ccccagctct tgaaggtgtt gccgttctcc atctcgcggg
     1321 tccggatgtt ctcggggttg tccgcgacgc cgccagagga tcccgcgatg aagtagtagt
     1381 ggttcggata gctgggaccc gctgcggagg cgaagaagct gtccgagagg ccgtactcgc
     1441 gcgcccagtg ccagtagttc gggatctggt cctcgtcgaa gatcgtgtag ccgtacgtcg
     1501 acccgtggat ccccgtgccg aacccgtcca tctccccgtt gttcacgcac gtccggtgcg
     1561 cggcgagatc gtgcgggatg tcgccaggca gccactcagg gcatcggatc agcggtttct
     1621 cgacgccgtt ctcgacgccg accgtggtcc ccgcccgcac gccggggaac ttcccgaaca
     1681 ggttgttgaa gctccggttc tcgagcatca cgtagatgac gcgcttgatg ggccatcggg
     1741 tgtcggtctc cgggatcgtg tcgtcgacct gcgggatccg ggtcccgccg gtgcacgaga
     1801 agaggacccc gccggcgacg gccatcgcgc ttgtctgcag gaaacgccga cggctgagcg
     1861 gcatcgggtg tcgctcgtcg ctcgtctccc cgtacggtcc gatgtccatg gccccctttt
     1921 ctatcaagcc cgttatcctc ggtcggttcc cgaggggtca tctcatgagg aaacacatga
     1981 tcgctgccgc gctgctgctc ttcctggttg cgtgcgacgg cggaccggag ccgttcccgc
     2041 caccgtcctc gccgacgtcg gcatccccgt tcccgtcgac gccgacgctc gcgtctccta
     2101 caccgccggc actcccctcg aacccgcttc cgaagagcgg tgatgcgtcg gagacctgtg
     2161 tcgagggctg ggtctcgccc ggcgtggagt cggc
//
LOCUS       DASUVE010000038         3105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_587658, whole genome shotgun sequence.
ACCESSION   DASUVE010000038 DASUVE010000000
VERSION     DASUVE010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587658"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..873
                     /locus_tag="VF036_00755"
     CDS             82..873
                     /locus_tag="VF036_00755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6263788.1"
                     /translation="MLLKDKVAVIYGAGGAIGSAVARAFASEGADLFLIGRSLALVEV
                     VAKEIAAAGATAEAAQVDALDEHAVDEHLDAVASKTGRVDISFDAIGVSNDTILGVPL
                     VDLDVERFFQPITTHATSYFLTARLAARRMIPSESGVIMTVTASHSRTGIPLVGGYGP
                     AQAVKESLTRSLSAELAPHGIRVVGLRPQAMPETETIREAYEPRAEASGMTWEQWQAA
                     LASRTHPRRLMTLEEMADVAAFVASDKASGMTGTTVNLTMGSLDD"
     gene            888..1637
                     /locus_tag="VF036_00760"
     CDS             888..1637
                     /locus_tag="VF036_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020119285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEX6263789.1"
                     /translation="MTDHRIATREDWNAAREKLLIREKEHTRLGDELAQERRDLPWLP
                     VDEEYRFDTEDGERTLAELFDGRSQLLVYHFMFGPSYEAGDPVNSSIADAVDAVLPHL
                     HARDVTFVFVSQAPLEKIQAYRRRMGWSFPWVSSAPSGFNFDLGFSHTPDEGRELVEE
                     MMKGGIPSIVDHNARSTGTDAAGYMTESPGFSAFVLEDGTVYQTYATTWRGLEFVMTY
                     YPILDHAPKGRGEGEDDWQLWLRRRDEYGGD"
     gene            complement(1835..2509)
                     /locus_tag="VF036_00765"
     CDS             complement(1835..2509)
                     /locus_tag="VF036_00765"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6263790.1"
                     /translation="MRARTVIVLGAAATVALLVRRHLRGPMGRETPRGILVGDAALYD
                     SMGHRLLGPFFDGIAADIAATAPAGARVLEVGCGPGRLAIRLARDHGLDVVGLDLDPA
                     MVLRAVANAARVRSAGREPLFVVGDVASLAFPDRSFDLVVSTLSMHHWTDHGGSLNEI
                     ARVLRPDGRALVWDLRRGPLPLHGELPDPTEHIHGTSLRLVGATPWRWPFGFRLTERL
                     ELVPGT"
     gene            2830..>3105
                     /locus_tag="VF036_00770"
     CDS             2830..>3105
                     /locus_tag="VF036_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263791.1"
                     /translation="MAIDPDVRRLEPIVGRWRSEGHVVGDPPIPIEGTDVYEWLPGGF
                     FLVHHVDVTVGGEPVRGIEMIGELDPVTGSLVARSYDDTGSVTIMHVS"
BASE COUNT          545 a         1038 c         1073 g          449 t
ORIGIN      
        1 gctcggagga atgggcggcg ggtgaccgcc caatccaacg cccctccggg gtccaaccaa
       61 acgagaggaa ggagtcaaac gatgttgctc aaggacaagg tggccgtgat ctatggagcc
      121 ggaggcgcga tcggtagcgc cgtcgcacgc gccttcgcat ccgagggagc cgatctcttc
      181 ctcatcgggc gttccctggc actcgtcgag gtcgtcgcca aggagatcgc cgccgccggc
      241 gcaaccgccg aggcagcgca agtcgacgcg ctcgacgagc acgccgtgga cgagcacctc
      301 gatgccgtag caagcaagac aggccgcgtc gacatctcgt tcgatgcgat cggcgtctcc
      361 aacgacacga tcctgggtgt cccgctcgtc gacctcgacg ttgagcggtt cttccagccg
      421 atcacgaccc acgcgacgtc gtacttcctg accgcgcgtc tggccgcgcg ccggatgatc
      481 ccgagcgagt cgggcgtgat catgaccgtc accgcgtccc actcgcggac gggcatcccg
      541 ctggtgggcg gctacggtcc ggcccaggcc gtcaaggagt cgctcacacg gagcctctcc
      601 gccgagctcg cgccgcacgg catccgagtg gtcggccttc gaccgcaggc gatgcccgag
      661 acggagacga tcagggaggc ctacgagccc cgcgcggagg cgtcggggat gacctgggag
      721 cagtggcagg cggcgctcgc cagccgaacc caccctcgac ggctcatgac gctcgaggag
      781 atggccgacg tggcggcctt cgtggcgtcc gacaaggcga gcggcatgac cggaacgacc
      841 gtgaatctga cgatgggaag cctcgacgac tgaagggaga ggaaacgatg accgaccaca
      901 ggatcgcgac gcgagaagac tggaacgccg cacgcgagaa gctgctgatc cgcgagaagg
      961 agcacacgag gctcggcgac gagctcgcgc aggaacgacg cgacctgccg tggctgccgg
     1021 tggacgagga gtaccggttc gacaccgagg acggcgagag gacgctggcc gagctgttcg
     1081 atggccgatc ccagctgctg gtctaccact tcatgttcgg accgagctac gaggccggcg
     1141 acccggtcaa ctcgtcgatc gccgacgccg tcgacgccgt gctcccgcac ctccacgccc
     1201 gcgacgtgac gttcgtgttc gtctcgcagg cgccgctgga gaagatccag gcctaccggc
     1261 gccgcatggg ctggagcttt ccctgggtct cctccgcgcc cagcggcttc aacttcgacc
     1321 tcggcttctc acacacgccg gatgaaggcc gcgagctcgt ggaggagatg atgaagggag
     1381 ggatcccgtc gatcgtcgat cacaacgccc gctcgaccgg caccgacgcc gccgggtata
     1441 tgaccgagag tcccgggttc agcgcgttcg tgctcgagga cggcactgtc taccagacgt
     1501 acgcgacgac ctggcggggg ttggagttcg tgatgaccta ctacccgatc ctcgaccacg
     1561 ccccgaaggg gcgcggcgag ggcgaggacg actggcagct ctggctccgc cgccgcgacg
     1621 agtacggggg cgactagagc aacgacctgc tcgatcgtgc cgttggggcg gctccgaaag
     1681 gagctgtacg agctctgtca acatgggggc cgcccaccta tcaccggcga ccacgacctc
     1741 ctatgcgact gggtggccaa gtgacgtgcc acgtgaccgg gccggagggg agatcagccc
     1801 ggcggatact gggaggtgga cgaggttcag ccgcctacgt ccccggcacg agctccaagc
     1861 gctcggtgag cctgaagccg aacggccatc gccacggcgt cgcacccacc agtcggaggg
     1921 acgtgccatg gatgtgctcg gtcggatccg ggagctcgcc gtggagcgga agcgggcctc
     1981 gccggagatc ccacacgagc gcccggccgt cgggacggag gaccctggcg atctcgttca
     2041 acgagccccc atggtccgtc caatggtgca tcgagagcgt gctcacgacg aggtcgaagg
     2101 atcggtcggg gaacgccagt gaggcgacat ctcccacgac gaacaacggt tcgcggccgg
     2161 cgctgcgcac gcgtgcggcg ttcgcgactg cccgcaagac catcgccgga tcgaggtcga
     2221 ggcccacgac gtcgagcccg tgatcccgcg ccaaccggat cgccagccgc ccgggaccgc
     2281 accccacctc caggacacgg gcgcccgcag gtgcggtggc ggcgatgtcc gccgcgatcc
     2341 catcgaagaa cggaccgagg aggcggtggc ccatcgagtc gtagagcgcg gcgtcaccga
     2401 ccaggattcc ccgaggggtc tctcgtccca tcggcccgcg gaggtgtcgt cgcaccagga
     2461 gggcgaccgt ggccgcggct cccaagacga tcacggttcg agccctcatc gctgtccaac
     2521 gtacgacccg cggtctcgta cgacttcagg gagccgttcg aacggcgaag cgcccgacgg
     2581 tcaacgtcag ttccgcccaa ggggtcgcga tcgtcgccgt ggcgctctcg ttcacgatcg
     2641 gggcggcaga aggggcggcg ctggagggtt cgcgaacgag gaacgccggc gccgcgtcgt
     2701 gaacgcggac tcgcggccct gagagtcgtc tcggtcgttc tcccgtggat ctcttgcgat
     2761 gttcacgaca tcgccgacgg atgcgagtgc gcgagtcacg gacgaaggcc gtcccttagc
     2821 ctccacaaga tggcgatcga ccccgatgtc aggcgcctgg agcccatcgt cggacgatgg
     2881 cgttccgagg ggcacgtggt cggtgatcct ccgatcccga tcgaaggcac agatgtctac
     2941 gagtggctcc ccggcggctt cttcctcgtc caccacgtcg acgtgacggt cggcggggag
     3001 cccgtgcgcg ggatcgagat gatcggggag ctcgatcctg tgacgggttc gttggtcgct
     3061 cgttcgtacg acgacaccgg aagcgtgacg atcatgcacg tctcg
//
LOCUS       DASUVE010000039         3192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1341551, whole genome shotgun sequence.
ACCESSION   DASUVE010000039 DASUVE010000000
VERSION     DASUVE010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341551"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..620
                     /locus_tag="VF036_00775"
     CDS             81..620
                     /locus_tag="VF036_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263792.1"
                     /translation="MPDSPQDLLVQWVLGCPIVGALLLVGGARAAGTALVGSSVLCFL
                     AFLLKAYYGGDHLDTLVHGAVILGSVGLVGFGLAGLALGSHAPLGRYPMALVLAFATV
                     VVGTLLILRIHAEAARICYPCFRGFEDFWVQALLAFNVAFLALLFLVPAWRASRSSRE
                     HEEDDPLVKLLIPRGGPPF"
     gene            920..1201
                     /locus_tag="VF036_00780"
     CDS             920..1201
                     /locus_tag="VF036_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263793.1"
                     /translation="MANTPEPSIERRRGKLVIEVPRLPSRPTLRECRARAKVIAARKE
                     GVRSEPDVTAYDGRPSAVGGPMRDPQGWIFTFDLNGGGSKQEGWSTTDG"
     gene            complement(1467..2888)
                     /locus_tag="VF036_00785"
     CDS             complement(1467..2888)
                     /locus_tag="VF036_00785"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX6263794.1"
                     /translation="MLAFGASILAFLVGFVYTSSSGQEADAAPLPTISVDDVTITEGS
                     GGTVSVVFHLTQNKRGKSTVRFATREGTAKAPGDFVGKSGQIRFAGNRLTKNVSVTVV
                     GDTLDEANETFFLELSNPVGATITDDRGEATITDDDASPTLSIVPSMSVPEGNTGDQP
                     SASLDVTLSAMSGRDVSVSFATVDVTATTADGDYVAKSGTIDFAPGETQRTIVVTVIG
                     DDDVELDETFDVQISSPVHATLGNATAVVTITGNDPIPPGSAVLNVSGASVREGALGI
                     TRVLTFTIVRSGETTTAVDVDFATTNGSATDPSDYLAASGTVAFGANETSATVDVTIV
                     GDRRLERKETFFLSLLDPSVGAAIESGQAAGHIVNDDTRTTLVVTKGARRILARGRLS
                     PARPGNHMVVRLFRKRNGVWVRLQTRRPALRGRTDLNGDGFTDSAYRTRFQRPRPGAC
                     RVIAQFVGGTTFGSSKAIRAFRC"
BASE COUNT          498 a         1048 c         1067 g          579 t
ORIGIN      
        1 gtacggctgg ccgcagacgt tcgtggcggt tctgtcgcgg ccgttgctca gggatacatg
       61 ctcgtgcttc catccgactg atgcccgaca gtccacagga tctccttgtt cagtgggtgc
      121 tcggctgccc gatcgtggga gcactcctgc tcgtcggcgg ggcgcgcgcg gcggggacag
      181 ctctggtcgg ctcctcggtt ctctgcttcc tcgccttcct cttgaaggcg tactacggag
      241 gcgaccacct cgacaccctc gtgcacggcg cagtcatcct ggggagcgtc ggcttggtcg
      301 ggttcggcct cgcagggctc gccttggggt ctcacgcccc gctcggtcga tatcccatgg
      361 cgctggtcct ggcattcgcc accgtcgtcg tcgggacgct gctgatcctc cggatccacg
      421 cggaggcggc tcggatctgt tatccgtgct tccgggggtt cgaggacttc tgggtccagg
      481 ccctgcttgc gttcaacgtc gccttcctcg cgctgctatt cctggtgccg gcgtggcgag
      541 cgtcgcggtc atcgcgggag cacgaagagg acgaccccct cgtgaagtta ctgattcctc
      601 gtggcgggcc gccgttctga cctgacgcta agcccctctc cagggacatc gtcgccgcga
      661 tggcctagcc tccccgtcgt agtggcgatg ttccgcaaga tgctcgacga agatcgacgc
      721 cgcgcgggca ggtcgaagtc aggccgcgtg gcggcgatcg cgtccgacgg ctttggacag
      781 ccgcacggcg cagagggcgg ctcgtggagc tgcttgaggc tgttcccgta cccgcgatgt
      841 cgccgaccgg ctcgcgaagc tcttaccgat gaccgacctg gtataccggt aactaggtag
      901 gcgcggagag gaggcgggta tggccaacac ccccgagcct tccatcgagc gccgcagggg
      961 aaagctcgta atcgaggtcc cgcgcctgcc gagccgcccg acgctccgcg agtgtcgcgc
     1021 ccgagccaag gtgatcgcgg cgaggaaaga aggcgtcagg agcgagccgg atgtgaccgc
     1081 atacgatggt cggccgtccg cggtcggggg tccgatgcgg gacccgcagg gatggatctt
     1141 cacgttcgat ctgaacggcg gtggctcgaa gcaggaaggg tggtcaacta ctgatggctg
     1201 aattgccatg caaacggcag cgcataactt cagccgtttc gctcagccgt ggccagcttc
     1261 tccgcaggtc agcacaccgt ttaggtcgcg gtgcgtctga agcaagcgct ctcccactga
     1321 gctacgcgcc ctcaaccgag gaggataggc caccgatcgc ggaaggtgac ctccactcgt
     1381 tcctccaggg gagcggaccc accggccgat cgaaccactc ggccgacggc ccgcaaccac
     1441 ctatcgctgc ctgagctggc gcccagtcaa caacggaagg ccctgatggc cttgctggac
     1501 ccgaaggtcg ttccgccgac gaactgggcg atcactctgc aggcgcctgg tcgcggtctc
     1561 tggaaccggg tgcgataggc gctgtccgtg aacccgtccc cgttcaggtc ggttcgcccg
     1621 cgcaacgccg gccggcgagt ctggagtcgg acccacacac cgttcctctt ccggaagagc
     1681 cgcaccacca tgtggttgcc gggtctggcg ggactgagtc ggccacgcgc gaggatgcga
     1741 cgggctccct tcgtgacgac gagggtcgtg cgtgtgtcgt cgttgacgat gtgccccgcc
     1801 gcctgtccgc tttcgatcgc cgcccccacg gaaggatcga ggaggctgag gaagaacgtt
     1861 tccttgcgtt cgaggcggcg gtccccgacg atggtcacgt cgaccgtcgc ggatgtctcg
     1921 ttcgctccga atgcgacggt gccactcgcc gccaggtagt cggacgggtc ggtcgccgat
     1981 ccgttcgtcg tcgcgaagtc cacgtccacg gcagtcgtgg tctctcccga ccgcacgatg
     2041 gtgaacgtga ggacacgcgt gatgccgagc gcgccctcac gcacggaggc ccccgagacg
     2101 ttcaagaccg ccgaacctgg cgggatcgga tcgttgcccg tgatcgtgac caccgcggtc
     2161 gcgttcccga gggtcgcgtg gacgggcgat gagatctgga cgtcgaacgt ctcatccagt
     2221 tccacgtcgt cgtcgccgat caccgtgacc acgatcgtcc gctgcgtctc tcccggagcg
     2281 aagtcgatcg tacccgactt cgccacgtag tccccgtccg ccgtcgtcgc cgtgacgtcg
     2341 accgtggcga acgacaccga tacgtcccga ccgctcatcg ccgacaacgt gacatcgagg
     2401 gacgcggacg gctgatcgcc ggtgttgccc tccggcaccg acatgctcgg cacgatcgag
     2461 agggtgggcg atgcgtcgtc gtccgtgatc gtcgcctctc cgcgatcgtc cgtgatcgtg
     2521 gccccgaccg ggttcgagag ctcgaggaag aacgtctcgt tcgcctcgtc gagggtatcg
     2581 ccgaccaccg tcacggatac gttcttggtg agtctgttgc cggcgaaacg gatctggccg
     2641 gacttcccga cgaagtcgcc cggcgccttc gccgttcctt ccctcgtcgc gaatcgaacc
     2701 gtggacttcc ctcgcttgtt ctgtgtgagg tggaagacga cggagaccgt ccccccgctg
     2761 ccctcggtga tcgtcacgtc gtcgacggag atggtcggca ggggggcggc atcagcttcc
     2821 tggcccgaag acgacgtgta gacgaaccca acgaggaagg cgaggatgga cgctccgaag
     2881 gcgagcatgc gggatcggga tgagcggatc acgatggagg cactcctctc agctgacggt
     2941 gaacgttcct ctctgctgta ggtaagtcgg cggcccccca ttcgaccttg agcagttcga
     3001 gtgatctcac catctcggta ccggaaatcg ggtagtgatg atcaagtagg accagaccat
     3061 tggacgggtc agggccgggg agcttcgcgg aggtgcccgt cggccatcat gagcacgcga
     3121 tcgagaagcc ggacgacctc ggggttgtgg gtggcgacca ccacggccgt ccccgcgtcg
     3181 gccgcccggc gg
//
LOCUS       DASUVE010000040         4826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_776271, whole genome shotgun sequence.
ACCESSION   DASUVE010000040 DASUVE010000000
VERSION     DASUVE010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776271"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VF036_00790"
     CDS             <1..882
                     /locus_tag="VF036_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6263795.1"
                     /translation="LPAGDPARRAVGRRTDPQQIANAREAFGLDQPLIVQYGRFAKGL
                     IPLPNTFLTEDVYYSFSNYLPVREEIWRRLPVSAILGMGAAVLWLLIGIPLGIASGVR
                     PRSLSARWTMILAIVGVSLPVFWLGQLLLYVVWYKLHLAPSAGLEINASVWGSALNGK
                     FLLPWITVAVGYAAIYARMLRSNMVETTQEDYIRTARAKGLSERRVVYKHALRGALTP
                     IVTMLGIDFATILAGLVITETLFGLPGLGQLAVRAIYSNDFPMVMGVTVVGSMFILAA
                     NIVVDIAYAFLDPRVRY"
     gene            1045..2481
                     /locus_tag="VF036_00795"
     CDS             1045..2481
                     /locus_tag="VF036_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HEX6263796.1"
                     /translation="MADAYQMFIDGEWVNSSDGQTRDVINPATGEVIASVQEGTKEDA
                     NKAVAAARKAFEGDWYDSTPKDRQLALLKLADRIEEQADEIVKLEAQNVGKPAAVTLS
                     EEIPPIVDTLRFFGGAARTMEGRSAGEYMAGFTSYVRREPIGVAGLIAPWNYPLMMAI
                     WKIGPALATGNAIILKPSEMTPLTALKLAELTADILPPGVFNVVTGDGVPVGDSLVRH
                     PDVGIVSLTGDTATGKLIAANSAETLKRVHLELGGKAPFIVFDDADMEQVVEWIKIGG
                     YFNSGQDCTASARILVGSGAYENVLAELVPAVESIKVGDPFADDTEMGSMVSKEQLER
                     VVGFVERAEEAGAKVLTGGKAIDQDGFWYSPTVVSDVGQDAEIIQKEVFGPVVTVQRF
                     DDEAQALEWANGVPYGLSSSVWTRDVGRAHRMARRLQFGCVWINTHIPLTPEMPHGGY
                     KQSGHGKDMSVYSLEDYTNMKHVMVSLD"
     gene            2514..3941
                     /locus_tag="VF036_00800"
     CDS             2514..3941
                     /locus_tag="VF036_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019608011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6263797.1"
                     /translation="MAVRPYLVNGEWRTGDGSFEVHSPYDDSVVAEIGVPTEADVEEA
                     AATAHETFEESSKLPVHARAEALDHVSKRLGETIEENAQLIAQEGGKPLKWATVEATR
                     AVSTFRWASEVIRRGDDELIRLDTEAALGSRVGLIRRFPIGPVLGITPFNFPLNLVAH
                     KVAPSLGVGAPIVVKPASATPIGSLRLSEFFAETDLPKGMYQVLPVSSRVADGMARDE
                     RYRKISFTGSAEIGWYLKGLDPKKRVTLELGGNAGVIVHADADLDFAAQRIAFGGYYQ
                     AGQSCISVQRVLVSSEVYDDFTARLVKQVESLKVGDPMDPTVDVGPVIQRSEVDRIAG
                     WVKEAVSEGAEVLTGGEGDGPFFQPTLISKVTPEMKVCREEVFGPVVTISPYQTFEEA
                     ITIVNDSKYGLQAGVFTNDINRAFEAHRSIEVGGVIVNDVSAFRADQMPYGGSKDSGF
                     GREGLRYAMEEMTEPRIMVLSHVPL"
     gene            3950..4330
                     /locus_tag="VF036_00805"
     CDS             3950..4330
                     /locus_tag="VF036_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263798.1"
                     /translation="MDARDVLLVLPFVVLITLGLGFLLVFRERAAAQEVLRRRSQLAE
                     PDLATPLRDSQPAERPWWGNPWLWVLVSVAFVALGIFVWPGLFGGTFLFLPFIWVWRP
                     RRGREGDPRSNGHGSRVDPGSLGG"
     gene            complement(4363..>4826)
                     /locus_tag="VF036_00810"
     CDS             complement(4363..>4826)
                     /locus_tag="VF036_00810"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX6263799.1"
                     /translation="TLVFNFRGTMNSAGTYGGGRDELRDVRAAIGTVRERAEGPTLVF
                     GWSFGASVALRECFDDERVSALVLFGAPLRPNDLQLPPMPTAAELRLLKRPLLLLAGA
                     NDEYCPADELRAYGDGVAEVAIMDGTDHYLWRQEREAAAIVGEFADRNLES"
BASE COUNT          749 a         1589 c         1731 g          757 t
ORIGIN      
        1 ctgccggcgg gcgatccggc gcggcgggcg gtcggtcgga ggaccgatcc gcagcagatc
       61 gcgaacgcgc gggaagcctt cggcctcgac cagcccctga tcgtccagta cggccggttc
      121 gcgaaggggc tgatcccgct gccgaacacg ttcctgaccg aggacgtcta ctactcgttc
      181 tcgaactacc tgccggtgcg cgaggagatc tggcgccggc ttcccgtctc ggcgatcctc
      241 gggatggggg cggccgtgct ctggctgctg atcgggatcc cgctcgggat cgcctcgggc
      301 gtccgacctc gatcgctgag cgctcgatgg acgatgatcc tcgcgatcgt gggggtgtcg
      361 ctgcccgtgt tctggctcgg ccagctgctg ctgtacgtgg tctggtacaa gctccacctc
      421 gcgccgtcgg cgggactgga gatcaacgcg agcgtgtggg ggagcgcgct gaacggcaag
      481 ttcctccttc cctggatcac ggtcgcggtc ggctacgccg cgatctacgc gcggatgctc
      541 cgcagcaaca tggtcgaaac gacccaggag gactacatcc gcaccgcgag ggcgaagggg
      601 ctctccgagc gccgggtcgt gtacaagcac gcgctccgcg gggcgctgac gccgatcgtg
      661 acgatgctcg gcatcgactt cgcgacgatc ctcgccggac tcgtcatcac cgagaccctc
      721 ttcgggctcc ccgggctggg tcagctcgcg gtccgggcta tctacagcaa cgacttcccc
      781 atggtgatgg gcgtgaccgt cgtagggtcg atgttcatcc tcgccgccaa catcgtcgtg
      841 gacatcgcgt acgcgttcct cgaccctcgc gtccgctact gaccacgtcg gtcggccaga
      901 tcggcgcacg gaggcgttcc gcgagaaccc atccggcccg ccggccggcg ggatgcacct
      961 cagcggacgg atgtcgcata ctggcgagcc gaacatcggt gcaggagccg cgcaggcagg
     1021 cggccgaacg aggaggggga acccatggcg gacgcctatc agatgttcat cgacggcgag
     1081 tgggtgaact cgtcggacgg acagacccgc gacgtgatca accccgcgac gggcgaggtc
     1141 atcgcctcgg tgcaggaagg cacgaaagag gacgcgaaca aggccgtcgc ggccgccagg
     1201 aaggcgttcg agggggactg gtacgactcg acgccgaagg accggcagct cgccctgctc
     1261 aagctcgcgg accggatcga ggagcaggcc gacgagatcg tgaagctcga ggcccagaac
     1321 gtggggaagc ctgccgccgt caccctctcc gaggagatcc cgccgatcgt cgacaccctc
     1381 cggttcttcg ggggcgccgc gcggacgatg gagggccgga gcgccggtga gtacatggcc
     1441 gggttcacca gttacgtccg tcgcgagccg atcggcgtgg cggggctgat cgccccctgg
     1501 aactacccgc tgatgatggc gatctggaag atcgggccgg ccctcgcgac ggggaacgcg
     1561 atcatcctca agccgtccga gatgacgccg ctcacggcgc tgaagctcgc ggagctgacg
     1621 gccgacatcc taccgcccgg cgtgttcaac gtcgtcaccg gcgacggcgt ccccgtgggc
     1681 gactcgctcg tccggcaccc cgacgtgggc atcgtctcgc tcaccgggga caccgccacc
     1741 ggcaaactga tcgcggcgaa ttccgcggag accctgaagc gggtccactt ggagctcggc
     1801 ggcaaggcgc cgttcatcgt gttcgacgac gccgacatgg agcaggtcgt cgagtggatc
     1861 aagatcggcg gctacttcaa ctccggtcag gactgcacgg cgtcggcgcg gatcctcgtc
     1921 gggtcgggcg cgtacgagaa cgtcctcgcg gagctggtgc cggcggtgga gtcgatcaag
     1981 gtcggcgacc cgttcgcgga cgacacggag atgggctcga tggtctcgaa ggaacagctc
     2041 gagcgcgtcg tcgggttcgt ggagcgggcc gaggaagcgg gcgccaaggt cctcacgggc
     2101 ggcaaggcga tcgaccagga cggcttctgg tactcgccca ccgtcgtgag cgacgtgggt
     2161 caggacgccg agatcatcca gaaagaggtc ttcggcccgg tcgtcaccgt ccagcgcttc
     2221 gacgacgagg cgcaggcgct cgagtgggcg aacggcgtgc cgtacgggct ctcgagctcc
     2281 gtgtggacgc gcgacgtcgg gcgggcgcac cggatggctc gccggctcca gttcgggtgc
     2341 gtgtggatca acacgcacat cccgctcacc ccggagatgc cgcacggcgg gtacaagcag
     2401 tccggccacg ggaaggacat gtcggtgtac tcgttggagg actacacgaa catgaagcac
     2461 gtgatggtgt cgctcgactg aacgagcgcg cgccccggaa aggcggcgat gacatggcgg
     2521 tgaggcccta cctggtgaac ggggagtggc ggacgggcga cggctcgttc gaggtccaca
     2581 gcccgtacga cgacagcgtc gtcgcggaga tcggcgtgcc gaccgaggcc gacgtcgagg
     2641 aagcggcggc gaccgcgcac gagacgttcg aggagagctc gaagctcccc gtccacgcgc
     2701 gcgcggaggc cctcgaccac gtctcgaagc ggctcggcga gacgatcgag gagaacgcgc
     2761 agctgatcgc ccaggaaggc ggcaagccgc tcaagtgggc gacggtcgag gcgacgcgcg
     2821 cggtgtccac gttccgctgg gcgtccgagg tgatccggcg cggggacgat gagctgatcc
     2881 gcctggacac ggaagccgcg ctcggctcgc gggtggggct gatccggcgg ttcccgatcg
     2941 gaccggtcct cgggatcacg ccgttcaact tcccgctgaa cctcgtcgcc cacaaggtgg
     3001 cgccgtcgct cggcgtggga gcgccgatcg tcgttaagcc ggcgagcgcg accccgatcg
     3061 gatcgctccg cctgtcggag ttcttcgccg agaccgatct cccgaagggg atgtaccagg
     3121 tgctgcccgt gtcgtcgagg gtggccgacg ggatggctcg cgacgagcgc taccggaaga
     3181 tctcgttcac ggggtctgcc gagatcggct ggtacctgaa gggcctcgac ccgaagaagc
     3241 gggtgacgct cgagctcggc gggaacgccg gcgtgatcgt ccacgcggac gcggacctcg
     3301 acttcgcggc gcagcggatc gcgttcggcg gctactacca ggccgggcag agctgcatca
     3361 gcgtgcagcg cgtgctggtc tcctccgagg tctacgacga cttcacggcg cggctcgtga
     3421 agcaggtcga gagcctgaag gtcggcgacc cgatggatcc cacggtcgac gtcggcccgg
     3481 tgatccagcg ctccgaggtg gatcggatcg cgggatgggt caaggaggcc gtctccgagg
     3541 gcgccgaggt gctcaccggc ggggagggcg acgggccgtt cttccagccc acgctgatct
     3601 cgaaggtgac gcccgagatg aaggtgtgcc gcgaggaggt cttcggaccg gtcgtcacca
     3661 tcagcccgta ccagacgttc gaagaggcga tcacgatcgt gaacgactcg aagtacggtc
     3721 tccaggccgg cgtgttcacg aacgacatca accgggcgtt cgaggcgcat cgctcgatcg
     3781 aggtcggtgg cgtcatcgtg aacgacgttt ccgcgttccg cgcggatcag atgccgtacg
     3841 gcgggtcgaa agattccggt ttcgggcgcg agggccttcg gtacgcgatg gaggagatga
     3901 ccgagccgcg gatcatggtc ctttcgcacg tcccgctcta ggcgggatcg tggatgcccg
     3961 cgacgtcctg ctcgtgttgc cgttcgtcgt actgatcacc ctgggtctgg ggttcctgct
     4021 ggtgttccgg gagcgggcgg cggcgcagga ggtcctgcgg cgtcggtccc agctcgcgga
     4081 gccggacctg gccacgccgc tccgcgactc gcagcccgcc gagcgaccgt ggtgggggaa
     4141 tccgtggctg tgggtgctcg tgtccgtggc gttcgtcgcg ctcggcatct tcgtgtggcc
     4201 cggcctcttc ggcggcacgt tcctgttcct gccgttcatc tgggtctggc gtccccgacg
     4261 cggccgcgaa ggcgacccac gttcgaacgg ccacggctcc cgcgtcgacc ccggctccct
     4321 cggcggctga cgcgccgcct tgcctctctc cgcctccctg gctcatgact cgaggttccg
     4381 gtcggcgaac tcgccgacga tcgcggcggc ctcgcgttcc tgtcgccaga ggtagtggtc
     4441 ggtgccgtcc atgatcgcga cctcggcgac gccgtcgccg taggcgcgca gttcgtcggc
     4501 ggggcagtat tcgtcgttcg ctcctgccag gagcaggagc ggtcgcttga ggagccggag
     4561 ctccgccgcc gtcggcatcg gcggcagctg gagatcgttc ggcctgagag gggcgccgaa
     4621 caacacgagc gcgctcaccc gttcgtcgtc gaagcattcc cgcagcgcga cgctcgcgcc
     4681 gaacgaccac ccgaagacga gtgtcggacc ctctgcgcgc tcccgcacgg tcccgatcgc
     4741 cgctcgcacg tctcgcagct cgtcgcggcc acccccgtac gtcccggccg agttcatcgt
     4801 gccgcggaag ttgaagacga gcgtcg
//
LOCUS       DASUVE010000041         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_23230, whole genome shotgun sequence.
ACCESSION   DASUVE010000041 DASUVE010000000
VERSION     DASUVE010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23230"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            431..559
                     /locus_tag="VF036_00815"
     CDS             431..559
                     /locus_tag="VF036_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263800.1"
                     /translation="MSPWFDFLQVSPDELSDLIDGTGWGIKRLVRDDGSYYVAVLA"
     gene            1195..1758
                     /locus_tag="VF036_00820"
     CDS             1195..1758
                     /locus_tag="VF036_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014058635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6263801.1"
                     /translation="MRSVTYSMNVSLDGYIVGPDGGFDWTAPDQEVFRFWIDEIREVG
                     VHLLGRRLYETMLYWETADQDRSLDDAELEWAALWNPLPKVVFSTTLSAVEGNARLAS
                     GGLAEEIERLRAEPGEGDIAIGGATLAAEAAALGLIDEYRAMVYPVLVGGGTPFFPQR
                     ERRVDLELVETRTFSSRFVYLRYRVAR"
     gene            1969..>2522
                     /locus_tag="VF036_00825"
     CDS             1969..>2522
                     /locus_tag="VF036_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010593318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6263802.1"
                     /translation="MRGAVMHGPGDVRVEQRPDPKIVEPTDAIIRLPATCICGSDLWP
                     YRGIEPIHAPVPMGHEYVGIVQEVGGAVSTIEPGQFVVGSFWASDNTCEICRAGYQSS
                     CISRELMGTIGTQAELARVPLADGTLVTTPDLPAEDLIPSFLAASDVLGTGWFGAVAA
                     DAGPGKTVAVVGDGAVGRLAVLAA"
BASE COUNT          359 a          819 c          894 g          450 t
ORIGIN      
        1 tgagcggaga gcgctccgct acgtccgtgg ccgcgtgttg gacgttggct gcggcgcggg
       61 acgagtggcg ctggaacttc aagcacgcgg tcgagaggtg gttgcgatcg atccctctcc
      121 cggagcggtg gaggtcaccc gtcggcgtgg cgtccgagac gttcgcctca tgcgactcga
      181 agatgtggac gcgtccctcg ggggtttcgg gaccgtgctg atgttcggca acaacttcgg
      241 gctcctcggt tctcgttcga gggctcgtcg gctgttgaga gggcttcgcc cgctcgccga
      301 caggatcgtg gctgggagca ctgacgtgtc gcgaacagag gatccactgc atctcgcata
      361 tgaacggcgc aaccgacgtc gggggcggat gcctgggcaa cggcgccttc gagtccggta
      421 ccgcaacctg gtgagcccct ggttcgactt cctccaggtg tcgcccgatg agttgagtga
      481 cctgatcgac gggactggat ggggcatcaa gcgcctcgtc cgtgatgacg gctcttacta
      541 cgtggcggtc ctagcgtgat gggctagaag gcgagttcag ggcccgatgt acgttcccgt
      601 cgcgtgaccg gtgtcgcggt catcgggtcc gcggacagct cgtggtcgcc aagacacaca
      661 tcgagcatgg tctgccctcg gtcaccctga cgggtcgtcc cggcgcggcg gtgcaggacg
      721 cacgcgatcg ggtccggcca gcagctggat ccttcagcgc tcatcaggcg ggcccgaact
      781 cctccccgtg gggcgtacgc acgaggagcc ttgtcggcgg gtcgagaggg tgggaggcgc
      841 ggacacgaga gcgtcgcagg ccgcgtcggc tcggacgacc tagtgaactc tctgggaatc
      901 gttcccacgc cggataactg cgttgtcggc ggctcgaacg cgttgggcgg gtccgtgttg
      961 agggaggagg ccgcgttgca cgtgatgggg ctccctcgct gggtcctggg cgtccgcacg
     1021 cgctccttgc gcgtcgcggc gctcccgctg tggccgggtg ggcggccggt cgcggatcgt
     1081 gcggaaggtc cgcccttgtc ggccctcgtc gcccgcctgg ggacccgcga gccgcgatga
     1141 gtcatgccgc gtccggcggt cgtagtgtca acccgttacc gaggaggact tctggtgcgc
     1201 agcgtgacct actcgatgaa cgtctcactt gacggctaca tcgtcgggcc ggacggcggc
     1261 ttcgactgga cggcgcccga ccaggaggtc tttcgcttct ggatcgacga gattcgagag
     1321 gtcggcgtcc acctgctggg acgacggctg tacgagacga tgctgtactg ggagaccgcc
     1381 gaccaggatc gatcgctgga cgacgcagag ctcgagtggg ccgcgctctg gaacccgctc
     1441 ccgaaggtgg tgttctccac cacgctgtcg gcggtggagg gcaatgcccg cctggcctcc
     1501 ggcggcctgg cggaggagat cgagcggttg cgagccgagc cgggggaggg cgacatcgcg
     1561 atcggcggcg cgactctcgc cgccgaggcg gccgcgttgg gtctgatcga cgagtaccgg
     1621 gccatggtct acccggtgct ggttggcggt ggtactcctt tctttcccca acgcgagcgc
     1681 cgggtggatc tcgaactcgt cgagacccgc accttcagct cgagattcgt ctaccttcgc
     1741 taccgcgtgg cgcgctagct ggctcgtccg ccgaccctca cggatgaggc gatagtcgat
     1801 gatcggcgcg ctttacgtcc tccgtatcca aggcaagcgt tcgatgcgtc cgcccttcgc
     1861 gtaactggag acgaaggcgc gtacctgccc acgttgcgca actgcccgac ctggtcccat
     1921 ccttctccta gagactcgtg gcgcgaggga tcggggaagg ccaagacgat gcgtggtgcg
     1981 gtgatgcatg gaccgggtga tgttcgtgtc gaacagcgtc ccgacccgaa gatcgtcgag
     2041 ccgaccgatg cgatcatccg actcccagcc acctgcatct gcggctcgga cctgtggccc
     2101 taccggggca tcgagccgat ccacgcgcct gtcccgatgg gtcacgagta cgtcggcatc
     2161 gtccaggagg tgggcggcgc cgtcagcacc atcgagcccg gccagttcgt ggtcggctcg
     2221 ttctgggctt cggacaacac ctgcgagatc tgccgggctg gctaccagtc ctcgtgcatc
     2281 agccgcgagc tcatgggcac catcggaacc caggcggagc tcgctcgcgt tccgctcgcc
     2341 gacggcaccc tcgtgaccac ccccgacctg ccggccgagg accttatccc gagcttcctc
     2401 gccgcctccg acgtcctcgg caccgggtgg ttcggcgccg tcgcggccga cgcgggaccc
     2461 ggcaagacgg tcgcggtcgt cggcgacggc gccgtcgggc gcctggcggt gctcgccgcg
     2521 aa
//
LOCUS       DASUVE010000042         7350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_23713, whole genome shotgun sequence.
ACCESSION   DASUVE010000042 DASUVE010000000
VERSION     DASUVE010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23713"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VF036_00830"
     CDS             complement(<1..560)
                     /locus_tag="VF036_00830"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX6263803.1"
                     /translation="MLGEQALERALAAAGLSAPVRWDDVTESTNATALALAAEGAPSW
                     TLVAASHQTAGRGRHGRVWRDRPGSALLCSLVLRPDWPPDRVGLVSLSAGAAMAEAIS
                     EIAGVEIRCKWPNDLMAGDTKIGGILGEAQVSAEAIVHVVVGIGVNIDVPEGVPGAGA
                     IGNVDEEALLASFLRRCSSMIGASAH"
     gene            597..2318
                     /locus_tag="VF036_00835"
     CDS             597..2318
                     /locus_tag="VF036_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX6263804.1"
                     /translation="MARKQKLLIANRGEIAIRIARTCRDLGIATVAVYSDADRDALHV
                     EIADESFRIGPGLASSSYLAIDRVMEVAVKSRATLVHPGYGFLAERAAFAEAVEEAGL
                     AFVGPPSQVIATMGDKAAARRVADAAGMPIVPGTREPVDLAQAKKQAARIGFPLMVKA
                     AFGGGGKGMHVVRDADHLEESLKRAAREAKSYFGRPEVFLERYVDRAHHVEAQVMADA
                     LGNVFFLGERDCSVQRRHQKLIEETPSPLFDERIRERFAEATTTLAREAGYVNAGTVE
                     CVMDEDGDFYFLEMNTRLQVEHTVTEMVTGLDLVALQIRVALGEELALDPAPRGVSIQ
                     CRINAEDPGRNFLPGPGRVTRYREPAGPFVRVDSGIEQGREIPGDYDSMFAKLIVSGH
                     DRETARIRMLRALDEFRVEGVPTTIPVHRWILESKEFRASRHTTTWLERALADAELPA
                     HPDLAPSGAPPNRPADILVEVDGRRVPVRIFDERREPAPRPPVTPDAHHGEHVHGEIL
                     APMQGTIVKVLVEKGQEVRPGDVICILEAMKMENHVPSTRDGEVTDLPIHAGQVVETG
                     QVLAVID"
     gene            complement(2315..3004)
                     /gene="npdG"
                     /locus_tag="VF036_00840"
     CDS             complement(2315..3004)
                     /gene="npdG"
                     /locus_tag="VF036_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX6263805.1"
                     /translation="MDVAVIGGTGAEGLGVAMRAAAAGHHVTIGSRDAGRGAETAAKA
                     AERVGAALEGTDNATAAAGAGGDGVVVVTVPYEGQAEIYRSIRDAVAPGAVILDATSP
                     LATAVGGRPWQVLRPWHGSTAEQAQAILGSGPRVVAGLHTIAAEALQALEQEVDSDVL
                     LCADDADAKARVGSLIDGIPGMRWVDCGRLEMARVAESLTALLVSINRTYKVKDSGFR
                     IVGRDAWGASP"
     gene            complement(3015..4595)
                     /locus_tag="VF036_00845"
     CDS             complement(3015..4595)
                     /locus_tag="VF036_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX6263806.1"
                     /translation="MSDEPSARTTDSGIEIEPIYEPHGGGVAAEIGEPGSFPFTRGVY
                     PDMHRGRLWTMRQYAGMGSADETNRRFRYLLDHGQTGLSVAFDLPTQMGLDSDHLRGE
                     GEVGKTGVAIDSIEDMERLLAGIPLDQVSTSMTINATAPILLLLYELVAEEQGLDPDT
                     LSGTVQNDLLKEYAARGTYIYPPQPSMRLITDLFAYAGERIPRWNTISISGYHMREAG
                     ATAAHEIAFTLANGIAYVRAAVDAGLAVDDVAPRLSFFFACHMHFFEEIAKFRAARRM
                     WAAIMRDRFGATDPRSLTLRFHTQTGGATLTAQQPENNIVRTALEAMAAVLGGTQSLH
                     TNSFDEALALPTEQAVKIALRTQQVLGFETGVAETVDPLGGSYYVEALTDELERIATE
                     IVATIDAMGGAVSAIEAGHYQDEIHEAAFRIQRGIETGDRIVVGVNRFVDADARAVEL
                     QRISAEETAQQMERLRELRASRDQPAVDAALLEVEETARGTGNLLPPMREALRLRSTL
                     GEVSDALRRVFGEYRPTY"
     gene            4704..5348
                     /locus_tag="VF036_00850"
     CDS             4704..5348
                     /locus_tag="VF036_00850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX6263807.1"
                     /translation="MDIVDPRIERYMEDRLRRFDEPVLLEMEAYAEEHGFPIVGRNVG
                     VTLEVLARSAGARRIMELGSGFGYSAYWHARAVGSDGEVHLTDGDPENEARAAGYLER
                     AGLADRVRFHVGDAVEELGKLDGDFDVVFNDIDKDGYPAAWRAASDRIRTGGLYVCDN
                     VLWYGRVAVEDPDDGRPHYTESVLEHNRLVAADERYVSTIVPTRDGVLVATRVG"
     gene            5398..5802
                     /gene="sdhC"
                     /locus_tag="VF036_00855"
     CDS             5398..5802
                     /gene="sdhC"
                     /locus_tag="VF036_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX6263808.1"
                     /translation="MSTETKRKGQFGPLYKGHEGQYSWLLHRVSGLAIILFLFAHVVD
                     TAVVGWGPDAYNRVASVYHNPFIRMLELGLVAAVLYHSFNGVKIMLIDFFPGLTRHIR
                     FLGIATALLFVGSMIPITWIMGSAIIELLLEG"
     gene            5806..6246
                     /locus_tag="VF036_00860"
     CDS             5806..6246
                     /locus_tag="VF036_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HEX6263809.1"
                     /translation="MSTTAEARPGTPLPQEKPVGAGRGRPVGGFELWAWLFMRISGLV
                     LLVLAVGHVLIMHVFGDGVDRVDFAFIQLRWASLFWKTWDWMMLVLALLHGVNGLRMI
                     LLDYVRPAGARRGINGFFVVLGAALMALGTVIVVTFDPAKWPTS"
     gene            6231..>7350
                     /locus_tag="VF036_00865"
     CDS             6231..>7350
                     /locus_tag="VF036_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6263810.1"
                     /translation="MADVVTITHTYDAVIVGAGGAGLRAAIEASGRVKTAVISKLYPT
                     RSHTGAAQGGVCAALGNVEEDYPEWHAFDTVKGGDYLVDQPAAQILTEEAVGAIYELE
                     RWGLPFNRTPEGLIDQRRFGGHTANHGERAVKRACYAADRTGHMILQTLYQQCVKRDV
                     RFFNEFYCLDLLLADDGRTAGVVAYELATGELHVFRAKSVLFATGGYGRMFRVSSNAH
                     ALTGDGLSVAFRRGIPLEDMEFFQFHPTGLYRLGVLLSEGARGEGAVIRNRDGERFME
                     RYAPTIKDLAPRDVVSRSIYQEIKEGRGLGPRGDYVLLDLTHLDPRVLEEKLPDITEF
                     ARVYLQVEPTTEPVPIQPTAHYAMGGIPTDVHARVVTGP"
BASE COUNT         1108 a         2590 c         2523 g         1129 t
ORIGIN      
        1 tcgtgggcgg acgccccgat catcgacgag catcgacgga ggaagctcgc gaggagggcc
       61 tcctcgtcta cgttcccgat cgcccccgcg cccgggacgc cctcgggcac gtcgatgttc
      121 acgccgatcc ccacgacgac gtgaacgatg gcctcggcgg agacctgcgc ttcaccgagg
      181 atccccccga tcttcgtgtc gccggccatg aggtcgttgg gccatttgca tcggatctcc
      241 acgcccgcga tctcagagat cgcttccgcc atcgccgccc ccgcggagag cgacacgagt
      301 ccgacccggt ccggcggcca gtcgggtctg agcacgaggc tgcacagcag cgccgatccg
      361 ggtcggtccc gccagacgcg tccgtgccgg ccccgaccgg ccgtctgatg cgaggcggcc
      421 acgagggtcc aggacggtgc cccctcggcc gcaagcgcca gcgccgtcgc gttcgtcgac
      481 tcggtcacgt cgtcccagcg gacgggcgcc gacagacccg ccgcggcgag cgcccgctcg
      541 agcgcctgtt cgcctaacac gtgtccaccg acctcgacgc tatatcgtgg cgccacatgg
      601 cgcggaagca gaagctcctg atcgccaacc gtggagagat cgcgatccgg atcgctcgga
      661 cgtgtcgcga cctcggcatc gccacggtgg ccgtctattc cgacgccgac cgtgacgctc
      721 tccacgtcga gatcgccgac gagtccttcc gcatcggacc gggactggca tcctcgtcct
      781 acctcgcgat cgaccgcgtc atggaggtcg ccgtgaagtc gagggcgacg ctcgtgcatc
      841 ccgggtacgg cttcctcgcg gaacgggcgg cgttcgccga ggcggtcgag gaggcggggc
      901 tcgcgttcgt cggcccgccc tcgcaggtga tcgcgacgat gggcgacaag gccgcggccc
      961 ggcgcgtcgc cgacgcggcg ggcatgccca tcgtgccggg gacgcgcgaa ccggtggacc
     1021 tcgcgcaggc gaagaagcag gcagcccgga tcgggttccc gctcatggtg aaggcggcgt
     1081 tcggcggggg cggcaagggc atgcacgtgg tgcgcgacgc cgaccatctc gaggaatccc
     1141 tcaaacgcgc ggcgcgcgag gcgaaatcgt atttcggacg acccgaggtg ttcctcgagc
     1201 gctacgtcga ccgggcccac cacgtcgagg cccaggtcat ggccgatgcc ctcggcaacg
     1261 tgttcttcct cggcgaacgc gactgctccg tgcagcgccg tcaccagaag ctgatcgagg
     1321 agacgccgtc tccgctgttc gacgagagga tccgtgagcg cttcgccgag gcgacgacga
     1381 ccctggcgag ggaggccggc tacgtgaacg ccggcacggt cgagtgcgtg atggacgagg
     1441 acggggactt ctacttccta gagatgaata cccggctcca ggtcgagcac acggtgacgg
     1501 agatggtgac gggcctcgat ctcgtggcgc tccagatccg cgtggcgctc ggcgaggagc
     1561 tggcgctgga ccccgctccg cgcggggtct cgatccagtg ccgcatcaac gccgaggacc
     1621 ccggcaggaa cttcctgccc ggcccgggcc gggtcaccag gtaccgggaa ccggcgggcc
     1681 ccttcgtgcg ggtcgattcc ggcatcgagc aaggccgaga gatccccggc gactacgact
     1741 cgatgttcgc caagctgatc gtatctgggc acgaccgcga gacggcccgg atccggatgc
     1801 tccgggcgct cgacgagttc cgagtggagg gcgtgcccac cacgatcccc gtgcaccggt
     1861 ggatcctcga gtcgaaggag ttccgagcct ccaggcacac gaccacgtgg ctggaacgcg
     1921 cactcgccga cgcggagctg ccggcgcacc cggatctcgc cccgtccggc gcccctccca
     1981 accggcccgc ggacatcctg gtcgaggtgg atggccgccg cgtcccggtc cggatcttcg
     2041 acgagcggag ggagcccgca ccgcgacccc cggtgacccc ggacgcgcac cacggcgagc
     2101 acgtccacgg cgagatcctc gccccgatgc agggcacgat cgtgaaggtc ctcgtcgaga
     2161 agggccagga ggtccgcccc ggcgacgtga tctgcatcct cgaggcgatg aagatggaga
     2221 accacgtccc gtcgacgcgg gacggcgagg tcaccgacct cccgatccac gccgggcagg
     2281 tcgtggagac cgggcaggtc ctcgccgtga tcgactaggg cgacgccccc caggcgtcgc
     2341 gaccgacgat ccggaacccg gagtccttca ccttgtaggt gcggttgatc gacacgagga
     2401 gcgccgtgag ggactcggcg acccgggcca tctcgaggcg gccgcaatcc acccatcgca
     2461 tgccggggat gccgtcgatc agcgagccga cgcgcgcctt cgcgtccgcg tcgtcggcgc
     2521 acagcagcac gtcgctgtcg acctcctgct cgagggcctg gagggcctcg gccgcgatcg
     2581 tgtggaggcc ggcgaccacg cggggtccgc tgccgaggat cgcctgggcc tgttcggccg
     2641 tcgacccgtg ccaggggcgg agcacctgcc agggccgccc gccgaccgcc gtggcgaggg
     2701 ggctggtggc atcgaggatc acggcgccgg gggcgacggc gtctctgatc gatcggtaga
     2761 tctcggcctg accttcgtac gggacggtga ccacgacgac gccgtcgccc ccggcgccgg
     2821 ccgccgccgt ggcgttgtcg gtgccctcca gcgcggcccc tacgcgctcg gcggccttcg
     2881 ccgccgtctc ggcaccacgc ccggcatccc gggacccgat cgtcacatgg tgaccggccg
     2941 cggccgcgcg catcgccaca ccgagtccct ccgcgcccgt cccaccgatc accgccacgt
     3001 ccacgatgcg cagcctagta ggtggggcga tactccccga agacgcgacg gagcgcgtcg
     3061 gacacctcgc ccaacgtcga tcgcagacgg agcgcctcac gcatcggcgg gaggaggttg
     3121 cccgtcccgc gcgccgtctc ctccacctcg aggagcgcgg catcgacggc cggctgatcc
     3181 cgggaggcgc ggagctcgcg gagtcgttcc atctgctgcg ccgtctcttc ggccgagatc
     3241 cgctggagct cgacggctct ggcatcggcg tcgacgaatc ggttcactcc caccacgatc
     3301 cgatcgcccg tctcgatccc tcgttggatc cggaacgctg cctcgtggat ctcgtcctgg
     3361 tagtggcccg cctcgatcgc ggagacggcg ccccccatcg cgtcgatcgt cgccacgatc
     3421 tcggtcgcga tccgttccag ctcgtcggtg agcgcctcca cgtagtacga gccgccgagc
     3481 gggtcgaccg tctcggccac gcccgtctcg aagccgagga cctgttgggt ccggagcgcg
     3541 atcttcaccg cctgctccgt ggggagcgcg agcgcctcgt cgaacgagtt ggtgtggagc
     3601 gactgggtcc cgccgagcac cgccgccatc gcttccagcg ccgtgcgcac gatgttgttc
     3661 tctggctgct gcgcggtgag cgtggcaccg ccggtctgcg tgtggaagcg cagggtcagc
     3721 gaccgtgggt ccgtcgcccc gaagcgatcc cgcatgatcg cggcccacat ccgtcgcgcg
     3781 gcgcggaact tcgcgatctc ctcgaagaag tgcatgtggc aggcgaagaa gaaggagagg
     3841 cgcggcgcca catcgtccac ggcgaggccg gcatcgacgg ccgcccgcac gtaggcgatg
     3901 ccgttcgcca gcgtgaacgc gatctcgtgc gcggcggtcg cgccggcctc gcgcatgtga
     3961 tacccgctga tcgagatcgt gttccagcgg gggatccgct cccccgcgta ggcgaacagg
     4021 tccgtgatca gtcgcatcga cggctgcggc gggtagatgt acgtcccccg cgccgcgtat
     4081 tccttcagca ggtcgttctg gacggtgccg gagagggtgt cggggtcgag gccctgctcc
     4141 tccgccacga gctcgtagag gaggaggagg atcggggcgg tggcgttgat cgtcatcgag
     4201 gtggatacct gatccagcgg gatccccgcg aggagccgct ccatgtcctc gatcgaatcg
     4261 atcgcgacgc ccgtcttccc cacctcaccc tcgcctcgaa ggtggtcgga gtccagcccc
     4321 atctgggtgg ggaggtcgaa ggccacggag agaccggtct gaccgtggtc gaggaggtag
     4381 cggaaccgcc ggttcgtctc gtcggccgac cccatcccgg cgtactgacg catcgtccac
     4441 aaccgcccgc ggtgcatgtc cgggtacacg ccgcgggtga aggggaacga gccgggctcc
     4501 ccgatctcgg cggccacccc gcctccgtgc ggctcgtaga tcggctcgat ctcgatcccg
     4561 gagtccgtcg tgcgagcgga cggctcatca ctcatggcgt cagggagcct atcgccggtg
     4621 ggacgcggga tggtcccgcg gtcacctccg ggagggccga gcggtcgaca cgatgttcgc
     4681 gacgacggta ccgttcccag cccatggaca tcgtcgaccc ccggatcgag cgctacatgg
     4741 aggatcgcct caggcgcttc gacgagcccg tgctcctgga gatggaggcc tacgccgagg
     4801 agcacgggtt tccgatcgtg ggccgcaacg tcggggtcac cctcgaggtc ctcgcccgct
     4861 ccgcgggcgc tcgtcggatc atggagctcg gttccggatt cggctactcc gcctactggc
     4921 acgcgcgcgc ggtcggttcg gatggagagg tgcacctcac cgacggcgat cccgagaacg
     4981 aggcgcgggc cgcagggtac ctcgagcggg cggggctcgc cgaccgggtc cggttccacg
     5041 tcggcgatgc cgtggaggag ctggggaaac tcgacggaga cttcgacgtg gtcttcaacg
     5101 acatcgacaa ggacgggtac cccgccgcat ggcgagcggc cagcgaccgg atccggaccg
     5161 gtggtctcta cgtctgcgac aacgtgctct ggtacggacg ggtagccgtc gaggatccgg
     5221 acgacgggcg accccactac acggagtccg tcctcgagca caacagactg gtcgcagcgg
     5281 acgagcgtta cgtctcgacg atcgtcccca cgcgcgacgg cgtcctggta gcgacccggg
     5341 tcggctgacc tccacggcag gccgcaccgc ggcgggccga cgataagctg agcctcggtg
     5401 agcacggaga ccaaacggaa gggccagttc ggcccgctct acaagggtca cgaaggccag
     5461 tacagctggc tccttcaccg ggtgagcggc ctcgcgatca tcctcttcct gttcgcccac
     5521 gtcgtggaca ccgcggtggt cggctggggg cccgacgcct acaaccgggt tgcctccgtc
     5581 taccacaacc cgttcatccg gatgctggag ctcgggctcg ttgcggccgt cctgtatcac
     5641 tccttcaacg gcgtgaagat catgctgatc gacttcttcc cggggctgac gcgccacatc
     5701 cggttcctgg gcatcgcgac cgcactcctg ttcgtcggat cgatgatccc catcacgtgg
     5761 atcatgggct cggccatcat cgagctcctc ctggagggct gacgcgtgtc cacgacggcc
     5821 gaggctcgtc ccggaacccc gctcccccag gagaagcccg tcggcgccgg acgaggccgc
     5881 ccggtcggag gcttcgagct ctgggcctgg ctcttcatgc ggatctccgg cctggtcctg
     5941 ctcgtcctcg cggtcgggca cgtcctgatc atgcacgtct tcggagacgg agtggaccgc
     6001 gtcgatttcg ccttcatcca gctccggtgg gccagcctct tctggaagac atgggattgg
     6061 atgatgctgg tgctcgccct gctccacggc gtgaacggac ttcggatgat cctgctcgac
     6121 tacgtgcggc cggccggtgc ccggcggggc atcaacgggt tcttcgtcgt cctcggcgcc
     6181 gccctgatgg cgctcggcac ggtcatcgtc gtcaccttcg accccgcgaa gtggccgacg
     6241 tcgtgacgat cacgcacacg tacgacgccg tgatcgtggg ggccggcggg gccggtcttc
     6301 gcgccgcgat cgaagcctcc ggccgggtca agacggcggt gatctccaag ctctacccca
     6361 cccgctcgca cacgggtgca gcgcaagggg gcgtctgcgc cgccctgggc aacgtggagg
     6421 aggactaccc ggagtggcac gcattcgaca ccgtgaaggg aggcgattac ctcgtggatc
     6481 agccggccgc acagatcctc acggaggagg ccgtcggcgc gatctacgag ctcgagcggt
     6541 ggggcctccc gttcaaccgg accccggaag gactcatcga ccaacgtcgt ttcggcggac
     6601 acaccgcgaa ccacggcgag cgcgccgtga agcgtgcctg ctacgcggcg gatcgcaccg
     6661 ggcacatgat cctgcagacc ctgtaccagc aatgcgtgaa gcgggacgtg cggttcttca
     6721 acgagttcta ttgcctcgac ctgttgctcg ccgacgacgg gcgtacggcc ggggtggtgg
     6781 cgtacgagct cgcgaccggc gagctccatg tgttccgggc gaagtccgtc ctcttcgcga
     6841 ccggagggta cggacggatg ttccgggtct cgtcgaacgc ccacgccctc accggggacg
     6901 gtctgtcggt cgcgttccgg cgtggcatcc ccctcgagga catggagttc ttccagttcc
     6961 atccgaccgg tctgtaccgg ctcggcgtcc tgctgtccga gggggcccgg ggcgagggcg
     7021 cggtgatccg caaccgcgac ggtgagcggt tcatggagcg gtacgcgccg acgatcaagg
     7081 acctcgcgcc ccgtgacgtc gtctcccgtt cgatctacca ggagatcaag gagggccggg
     7141 ggctcggccc gcgaggcgac tatgtcctcc tcgacctgac gcatctggat ccccgggtcc
     7201 tcgaggagaa gctcccggac atcaccgagt tcgcccgcgt gtacctccag gtcgagccca
     7261 cgacggagcc agtcccgatc caacccacgg cccactacgc gatgggcggc atcccgaccg
     7321 acgtgcacgc gcgcgtggtc accggacccg
//
LOCUS       DASUVE010000043         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_401162, whole genome shotgun sequence.
ACCESSION   DASUVE010000043 DASUVE010000000
VERSION     DASUVE010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401162"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VF036_00870"
     CDS             complement(<1..1004)
                     /locus_tag="VF036_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6263811.1"
                     /translation="MKARAVSIATAAGLAVVVFLVGALGHPGDPAMLPEPITRGASAD
                     AFLFPASGAGTLGTVIASLEERLDAVPDDWEASAALGTAYVQQARITSDPSAYPLARA
                     ALRRSLALQPNDNAGALVGLGSLAAARHDFADALRWGRRAVRAAPFDADAYGVLGDAL
                     LELGRYRAAFTSFQRMVDTRPDLASYARVSYALELRGNVAGAIDAMRAAFDVAASRPD
                     VAWTAAQIGKLHFGAGRIGDARAWFRRARAADPDSMEAAAALALVAWASGDLPAAIAG
                     YERLAARFPSPEHVATLGDLYAAAGDREAAAAQFDLVRAEARLFRANGVNTDLEIALF
                     "
     gene            complement(1066..>2256)
                     /locus_tag="VF036_00875"
     CDS             complement(1066..>2256)
                     /locus_tag="VF036_00875"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEX6263812.1"
                     /translation="GSWIPFEEPAGGPNFYLWAERTNYDINVDNDGDARPDVIFRWRF
                     TTHHRNPDSFIYNNGPVTSLNDENLLIYQTYDLTRIKGDNAVKLLDNARVVPSYVGDA
                     SMPGYNADLFDAGVRSAGSGRRSWVGQSDDPFFLDLRVFDLLYGGDFSEAGDDTLDGF
                     NVNTMVLEVPKSQLRGPDDGVIGVWTTASRPSIRIQGADGSIEWDGATVQVSRLGMPL
                     VNEVVVPIGLKDYFNGSNPRDDAALPKVNDPELPHLISAVYNMLFPEVPDSNGDKAGV
                     QRSDLVKVFLTGLPGLNKPDDVRAAEMLRLNMDIAPCSDACSTLGAIAGDAAGFPNGR
                     RLSDDIIDIALRVVMGVLIPGHDTDADGLADGVDENDASFLAEFPYVAYPHSGSDADP
                     HAVP"
BASE COUNT          352 a          775 c          802 g          327 t
ORIGIN      
        1 tcgaagagcg cgatctcgag gtcggtgttc acgccgttcg cgcggaagag gcgcgcctcc
       61 gccctgacga ggtcgaactg tgcggcggcc gcctcgcgat cccccgcggc cgcgtagaga
      121 tcgccgagcg tggccacgtg ctccggcgac gggaaccggg ccgccagccg ctcgtagccc
      181 gcgatcgcgg cggggaggtc gccggaggcc cacgcaacga gcgccagggc cgccgccgcc
      241 tccatcgagt ccgggtcggc ggcccgcgcg cggcggaacc acgcgcgagc gtccccgatc
      301 cggccggctc cgaagtggag cttcccgatc tgcgccgccg tccaggcgac gtccggccgg
      361 ctcgcggcca cgtcgaacgc cgcacgcatc gcgtcgatcg ctccggccac gttgccccgg
      421 agctcgagcg cgtagctgac cctggcatag gacgcgagat ccggacgggt gtcgaccatc
      481 cgttggaacg acgtgaacgc ggcgcgatac cgtccgagct cgagcagagc gtccccgagg
      541 accccgtagg catcggcatc gaacggagcg gcacggacgg cgcggcgtcc ccaccggagg
      601 gcgtcggcga agtcgtgccg cgccgccgcc agggagccga gccccacgag cgctccggca
      661 ttgtcgttgg gctggagcgc gagcgagcgg cggagggcgg ctcgggcgag cggatacgcc
      721 gatgggtccg acgtgatccg ggcctgctgg acgtaggcgg tcccgagggc ggccgaggcc
      781 tcccagtcgt cggggaccgc atcgagccgt tcctcgagcg acgcgataac ggtaccgagc
      841 gtgccggcac cggacgcagg gaacaggaac gcatccgccg aggcgccccg cgtgatcggc
      901 tcggggagca tcgccggatc gcccgggtgt ccgagcgcgc cgacgaggaa cacgaccacc
      961 gcgagtcccg cggcggtcgc gatcgagacg gctcgagcct tcatgacgat cctccgggag
     1021 gaggagtggc cgccgcagcg gccactcccc ttcctccttc gttcctcagg gcaccgcgtg
     1081 cgggtcggcg tccgacccgg agtgcgggta cgcgacgtac gggaactccg ccaggaacga
     1141 cgcgtcgttc tcgtccacgc cgtcggccag gccgtcggcg tccgtgtcgt ggccggggat
     1201 caggaccccc atcacgacgc gcagagcgat gtcgatgatg tcgtcgctca gacgacggcc
     1261 gttcgggaac ccggccgcat cacctgcgat cgcgccgagc gtcgagcacg cgtccgagca
     1321 cggagcgatg tccatgttca gtcgcagcat ctccgccgcc cggacgtcgt ccggcttgtt
     1381 cagccccggc agaccggtga ggaacacctt caccaggtcg gaccgctgga ccccagcctt
     1441 atcaccgttg gagtccggaa cctcggggaa caacatgttg tagaccgccg agatcaggtg
     1501 cggcagctcg ggatcattca ccttcggcaa ggcggcgtca tcgcgcgggt tggacccgtt
     1561 gaagtagtcc ttgagcccga tcggcacgac gacctcgttc acgaggggca tgccgagtcg
     1621 cgagacctgg acggtcgctc catcccactc gatggagccg tccgcgccct ggatgcggat
     1681 cgacgggcgg gacgccgtgg tccagacacc gatcacgccg tcatccggcc cacggagctg
     1741 gctcttcggc acctcgagca ccatcgtgtt gacgttgaag ccgtccagcg tgtcgtcgcc
     1801 ggcctccgag aagtcccctc cgtagaggag gtcgaagaca cggaggtcga ggaagaacgg
     1861 atcgtcggac tgaccgaccc agctccgcct gccgctgccg gcagaccgca cccccgcgtc
     1921 gaagaggtcg gcgttgtatc cgggcatcga cgcgtcgccg acatatgagg ggacgacgcg
     1981 cgcgttgtcg agcagcttga cggcgttgtc tcccttgatg cgggtcaggt cgtaggtctg
     2041 gtagatgagc aggttctcgt cgttcagcga cgtcaccggc ccgttgttgt agatgaagga
     2101 gtcggggttg cggtgatgcg tcgtgaaccg ccaacggaag atcacgtcgg gacgcgcgtc
     2161 tccgtcgttg tcgacgttga tgtcgtagtt cgttcgctcg gcccagaggt agaagttcgg
     2221 gccgcccgcg ggctcctcga acgggatcca cgagcc
//
LOCUS       DASUVE010000044         4863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_213113, whole genome shotgun sequence.
ACCESSION   DASUVE010000044 DASUVE010000000
VERSION     DASUVE010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213113"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /gene="ybeY"
                     /locus_tag="VF036_00880"
     CDS             <1..419
                     /gene="ybeY"
                     /locus_tag="VF036_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX6263813.1"
                     /translation="RGALDEEGLRSLARETLRGEGVDRAELSVSFVESGEIEELHERF
                     MDEPGPTDVLSFPLDEADGSAKDGAEDGDGIRHLGDVVVAPAEAERNNPADPEGELRL
                     LLVHGILHLLGYDHEEEDARTQMWERQEHYSGVRAS"
     gene            416..1693
                     /locus_tag="VF036_00885"
     CDS             416..1693
                     /locus_tag="VF036_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX6263814.1"
                     /translation="MNLWWGLAVVLVLFGSVLAAAEASLTRTSRVRALSLEEDDRRNA
                     ETLVKIESDPPRYLNAVYLSVMFVQNGSAILVAILAENEFGEGGDGPWVGLISFGFTL
                     LYFVFVEAMAKTFAVLHTDRVALTLAPLVWFLGRVLEIPTRFLIGLANVLLPGKGLKQ
                     GPFVSEEEIRSMAEVGSEEGSIEEGEKELIHSIFEFGDTIVREVMVPRPDIVAIEDDK
                     TLRDVQALVLSHGYSRIPVFHGSLDDVKGVVFAKDVLKALHQGKKDMPLGEIVRTAHF
                     VPESKRVAELLKEMQREKFHQALVYDEHGSVTGIVALEDLLEELVGEIADEYDREEPE
                     VLELGDGIYRVSGKASIDDVNELLGTELPDEEWDTVAGLMLDLFGRMPETGEEVAFRG
                     LSLKTDKVQGRRIASVLITRSLEENGEQPEVAT"
     gene            1690..2103
                     /locus_tag="VF036_00890"
     CDS             1690..2103
                     /locus_tag="VF036_00890"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX6263815.1"
                     /translation="MTSIEPDRIDELVAAAREIRAAAYAPYSGFHVGAALLAGERIFT
                     AVNMENASYPLSVCAERNAVAMAVAAGETSLRAVAVVTDAETEPTPPCGGCRQVLWEF
                     GEGMAVIAESASGTERRYWALADLLPDAFGPDDLT"
     gene            2100..2993
                     /gene="era"
                     /locus_tag="VF036_00895"
     CDS             2100..2993
                     /gene="era"
                     /locus_tag="VF036_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010551688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX6263816.1"
                     /translation="MISSAVTRSGIIAVVGRPNVGKSTLVNALVGRKVSIVSDKPQTT
                     RRGLHAVLTTEDAQAVFTDTPGFHKPRTELGARLNDMVGDAVEGVDVILHVLDAASGV
                     GRGDAFVYERRVRPHGCPKIAVVNKIDVTGQHGVVPQLAAAAEVGDYDEIVPVSAKAG
                     KGVGMLRDMVLAMLPEGPALYPEETISDQPLETRLAEMVREKALHVTRQELPHSITVT
                     IEELDITEALVKVHASLIVERDSQKGIVIGKGGQTLRTIGTRAREEMELILGTKVFLD
                     VRVKVVKEWQRDPKALERLGF"
     gene            complement(2997..3344)
                     /locus_tag="VF036_00900"
     CDS             complement(2997..3344)
                     /locus_tag="VF036_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6263817.1"
                     /translation="MKIARVILVALAAIVPACDGETAPPEACTDPAPATSVELADFAF
                     RPDCLSADAGGAITLDNTGDAPHTFTVEGTDVDVNLDAGSSGEADLGTVDPGTYTVTC
                     TFHPQMEATLTVG"
     gene            3467..4354
                     /gene="deoC"
                     /locus_tag="VF036_00905"
     CDS             3467..4354
                     /gene="deoC"
                     /locus_tag="VF036_00905"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015625572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX6263818.1"
                     /translation="MAVRTTAVDAVGLEERAASLAKRSIKRDAKLWALDLAIRSMDLT
                     TLEGSDTVGKIVAICAKAVRPDPLDPTIPPVAAVCLYPQLVPVALEQLDGTRVKVASV
                     AGGFPSGLGPLEARLQEIHDVAETGAHEIDIVLNRSLFLGGSYAQAFEELVAAREAAG
                     AAHLKVILETGELGSYDRVRQASMLAMAAGADFIKTSTGKIAVNATMPVALCMMEAAR
                     DFHRDTGRAVGIKVAGGVRTAKQAIQNLVLLHETLGSTWMTPDRFRIGASSLLNDVLM
                     QIEKERTGRYAGPDYFTID"
     gene            4357..>4863
                     /locus_tag="VF036_00910"
     CDS             4357..>4863
                     /locus_tag="VF036_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6263819.1"
                     /translation="MVVVRTMGTRVLSTSHGTHRGAFAPVDWALLTSIGTIWGSSFLF
                     MAIGLEAFQPGLVTWLRILFGVAVVGVLPRARRTSIAREDLPALFVVAISWVAIPFTL
                     FPLAQGSVNSAIAGMLNGAAPIFTAVITTILLRRPPGPRQLLGIPIGFLGVSAIALSA
                     ASEGSSQVV"
BASE COUNT          758 a         1598 c         1753 g          754 t
ORIGIN      
        1 agcggggagc gctcgatgag gagggactcc gctcactcgc ccgcgagacc ctgcgaggcg
       61 aaggggtcga ccgcgcggag ctctcggtgt ccttcgtcga gagcggcgag atcgaggagc
      121 tccacgagcg attcatggac gagccgggac cgaccgacgt gctctcgttc ccgctcgacg
      181 aggcggacgg atccgccaag gacggagccg aggacggaga tggcatccgc cacctcggcg
      241 acgtggtcgt cgcgccggcg gaggcggagc gcaacaaccc ggcggatccc gagggggagc
      301 tccgcctgct gctcgtccac gggatcttgc acctcctcgg ctacgaccac gaagaagagg
      361 acgcgcgcac acagatgtgg gagcggcagg agcactacag cggggtgcgg gcgtcgtgaa
      421 cctgtggtgg ggcctcgcgg tcgtgctggt gctgttcggc tccgtgctcg ccgccgccga
      481 ggcgtcgctc acccggacgt cgcgcgtccg cgcgctctcg ctcgaggagg acgatcgccg
      541 gaacgcggag acgctcgtga agatcgagtc ggatccgcct cgctacctga acgccgtgta
      601 cctctccgtg atgttcgtgc agaacgggtc ggcgatcctc gtcgcgatcc tcgcggagaa
      661 cgagttcggg gaaggcggcg acggcccgtg ggtgggcctg atctcgttcg ggttcacgct
      721 gctgtacttc gtgttcgtcg aggcgatggc caagacgttc gccgtgctgc acaccgaccg
      781 cgtggcgctc acgctcgccc cgctcgtatg gttcctcggc cgggtcctcg agatcccgac
      841 gcggttcctg atcggcctcg ccaacgtcct cctgccgggc aaggggctca agcaagggcc
      901 gttcgtctcc gaggaggaga tccgttcgat ggccgaggtg ggttcggagg agggatcgat
      961 cgaggaaggg gagaaggagc tgatccactc gatcttcgag ttcggcgaca cgatcgtccg
     1021 cgaggtgatg gtgccgcgcc cggacatcgt cgcgatcgag gacgacaaga cgctccgcga
     1081 cgtccaggcg ctcgtcctgt cgcacgggta ctcccggatc cctgtcttcc acgggtcgct
     1141 cgacgacgtg aagggcgtcg tgttcgcgaa ggacgtgctg aaagcgctgc accaggggaa
     1201 gaaggacatg cccctcggcg agatcgtgcg gacggcgcac ttcgtgccgg agagcaagcg
     1261 ggtggccgag ctgctgaagg agatgcagcg cgagaagttc caccaggcgc tcgtgtacga
     1321 cgagcacggc tccgtcaccg gcatcgtcgc gctcgaggac ctcctcgagg agctcgtggg
     1381 cgagatcgcc gacgagtacg accgagagga accagaggtg ctggagctcg gcgacggcat
     1441 ctaccgggtc tccgggaagg catcgatcga cgacgtgaac gagctgctcg gcacggagct
     1501 ccccgacgag gaatgggaca ccgtcgcggg cctgatgctg gacctgttcg gccggatgcc
     1561 cgagacgggc gaggaggtcg cgttccgcgg tctgagtctc aagacggaca aggtgcaggg
     1621 acgacggatc gcgagcgtgc tgatcactcg ctcgctcgag gagaacggcg agcagccgga
     1681 ggtggccacg tgacctcgat cgagccggac cgcatcgacg agttggtcgc cgccgcccgc
     1741 gagatccggg ccgcggccta cgctccgtac agcggcttcc acgtgggcgc ggctctgctg
     1801 gccggcgagc gcatcttcac ggccgtgaac atggagaacg cgagctaccc cttgtcggtg
     1861 tgcgcggaac gcaacgcggt cgcgatggcc gttgccgcgg gcgagacgtc gttgcgggcg
     1921 gtcgccgtcg tcacggacgc cgaaacggag cccactccgc cgtgcggcgg gtgtcgtcag
     1981 gtgctctggg agttcggaga agggatggcc gtgatcgccg agtcggcatc cggcaccgag
     2041 cgccggtact gggcattggc agacctcctg cccgacgcct tcggtccgga cgacctcacg
     2101 tgatctcctc cgcggtcacc cggtccggca tcatcgcagt cgtcggccgc cccaacgtcg
     2161 ggaagtcgac gctcgtgaac gcgctcgtcg gacgcaaggt gtcgatcgtc tcggacaagc
     2221 cgcagacgac gcgtcgggga ctgcacgcgg tcctcacgac ggaggacgcg caggccgtct
     2281 tcaccgacac ccccgggttc cacaagcccc gaaccgagct cggcgcccgg ctgaacgaca
     2341 tggtcggcga cgccgtcgag ggcgtggacg tgatcctgca cgtcctcgat gcggcctccg
     2401 gcgtcgggcg gggcgacgct ttcgtgtacg agcgccgggt ccgtccacac gggtgcccga
     2461 agatcgccgt ggtgaacaag atcgacgtca cgggtcagca cggcgtcgtg ccccagctcg
     2521 ccgcggccgc ggaggtcggt gactacgacg agatcgtccc ggtgtccgcc aaggccggga
     2581 agggtgtcgg catgctccgg gacatggtcc tcgcgatgct cccggagggt ccggcgctct
     2641 atccggagga gacgatctcg gatcagccgc tcgagacccg cctcgcagag atggtccgcg
     2701 agaaggcgct ccacgtgacc cgccaggagc tcccgcactc gatcaccgtc acgatcgagg
     2761 agctcgacat cacggaggcg ctcgtgaagg tccatgcatc gctgatcgtc gaacgcgact
     2821 cgcagaaggg gatcgtgatc ggcaagggtg ggcagacgct ccggacgatc ggcacgcggg
     2881 ctcgcgagga gatggagctg atcctcggga cgaaggtgtt cctcgacgtc cgggtgaagg
     2941 tcgtgaagga gtggcagcgc gaccccaagg cgctcgagcg gctcgggttc tgaccgtcac
     3001 ccgacggtga gggtcgcctc catctgcgga tggaacgtgc acgtcacggt ataggtcccc
     3061 ggatccacgg tcccgaggtc cgcctcgccc gaggatccgg cgtcgaggtt cacgtcgacg
     3121 tccgtgccct ccaccgtgaa cgtgtggggc gcatcgccgg tgttgtcgag cgtgatcgcc
     3181 ccccccgcgt cggccgacag gcaatccggg cggaacgcga agtccgccag ctcgaccgag
     3241 gttgccggag ccggatccgt gcaggcttcc ggcggagcgg tctcgccgtc gcaggcgggg
     3301 acgatcgcgg cgagggcgac gaggatcaca cgggcgatct tcatgcggtg gagggtaccg
     3361 acccggacct cgtgacgacc gggtgggcta ccatcgatgc atggtccgag cagcgcacat
     3421 cgagcccagc cgacccctcc ccgtcgctcc cgccgaggcc gtgcggatgg ccgtgcgtac
     3481 gaccgccgtc gacgccgtcg gcctcgagga gcgcgcggcg tccctcgcga agcgttcgat
     3541 caagcgcgac gcgaagctgt gggcgctgga tctcgcgatc cggtcaatgg acctcacgac
     3601 gctcgagggg agcgacaccg tggggaagat cgtcgcgatc tgcgcgaagg ccgtgcgtcc
     3661 ggacccgctc gacccgacga tccctcccgt ggcggccgtg tgcctgtacc cgcagctcgt
     3721 ccccgtcgcc ctggagcagc tcgacggcac cagggtgaag gtcgcgagcg tggcgggagg
     3781 cttccccagc ggactcggcc ccctggaggc gcgtctgcaa gagatccacg atgtggcgga
     3841 gacgggcgcg cacgagatcg acatcgtgct gaaccgctcc cttttcctcg gtgggagcta
     3901 cgctcaggct ttcgaggagc tcgtcgccgc ccgcgaggcc gccggcgcgg ctcacctgaa
     3961 ggtgatcctc gagaccggcg agctcgggtc ctacgaccgc gtgcggcagg cgagcatgct
     4021 ggcgatggcc gcgggcgcgg atttcatcaa gacgtccacg ggcaagatcg ccgtgaacgc
     4081 cacgatgccg gtggccctct gcatgatgga ggcggcgagg gatttccacc gggacacggg
     4141 ccgcgccgtc ggcatcaagg tggcgggcgg ggtccgcacg gccaagcagg cgatccagaa
     4201 cctggtcctg ctgcacgaga ccctaggttc gacgtggatg actccggacc gcttccggat
     4261 cggcgcgtcc tcgttgctga acgacgtgct gatgcagatc gagaaagaac ggaccggccg
     4321 ttacgcggga ccggactatt tcacgatcga ctgacgttgg tcgttgttcg gacgatgggg
     4381 acccgtgtgc tgtcgacctc gcacggaacg caccgcggcg cgttcgcgcc cgtggactgg
     4441 gcgctgctga cctcgatcgg cacgatctgg ggctcctcgt ttctgttcat ggcgatcggc
     4501 ctcgaggcgt tccagcccgg gctcgtcacg tggctccgga tcctcttcgg cgtggccgtc
     4561 gttggcgtcc ttccccgtgc ccgccggacg tcgatcgcgc gggaggacct gccggccctg
     4621 ttcgtcgtcg cgatctcgtg ggtggcgatc cccttcacgc tgttcccgct ggcacagggg
     4681 tcggtgaact ccgcgatcgc ggggatgttg aacggcgcag ccccgatctt caccgcggtg
     4741 atcaccacga tcctgctgcg gaggccgccc ggcccgcgcc agctgctcgg gatcccgatc
     4801 gggttcctcg gcgtcagcgc gatcgcgctg tccgcggcga gcgagggctc cagccaggtc
     4861 gtc
//
LOCUS       DASUVE010000045         4383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_402060, whole genome shotgun sequence.
ACCESSION   DASUVE010000045 DASUVE010000000
VERSION     DASUVE010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402060"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VF036_00915"
     CDS             <1..222
                     /locus_tag="VF036_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263820.1"
                     /translation="DGDRLTLFNDPNCSRDRGTYTWELERGSLHLEPIDDSCAYEGER
                     ATDLATEDWTRIPVCRREIAHLWPGILGC"
     gene            complement(232..2427)
                     /locus_tag="VF036_00920"
     CDS             complement(232..2427)
                     /locus_tag="VF036_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX6263821.1"
                     /translation="MVERRRTGTWLTWVAAGLIIGAVECVLAIAFAAFVFGGGALVND
                     LPQGIGLYLGAAALTLAFFAWRAGHRGVVGSVQDAAAAVLSLVAAAVAKKVAELEHVA
                     QQSGLQDFERPDIFLTVVAATFVVTVLCGIVFFVMGRFRLGSLVRFVPYPVVGGFLAG
                     TGWLLFKAGVNASTGMQVKLGNVLQLFEFQLSRLLPALTFGVILLIAVRVWKRPFVIP
                     AVIAIGLVSFVVWMIVTGQSIDEVRADGWLLFGPFQTSKPWEFVTPEALAGADWSSIL
                     ESWVTILDAVFVATLAILFNISGSELVLDRDLDTNQELRDAGILNVASGALGGIPGYH
                     ALSLTALPRYLKVDARAAGLIAAAVPLAAVAFAASVVGLIPRMIVGGVLIFIGLDFLV
                     GWVWDKRKVLPRLEYWVVVAILAGIIVWGFREGVVIGLVLAVMLFAINYGRIELVREV
                     SFGETYRSNVDRPAAERAELRTLSDRVQILRVSGFVFFGSTNRLLERIRRRVEEDTPP
                     RFLLIDLRRVTGVDSSAVVSFVKVLRLAEASGFEVVFTGASDPVRAQLARGGVEETPG
                     LVSFEPDLDRGLERCEDALLMDLVPGERTVPVLEGLPPHLWEYFERVSLEEGTKLIEQ
                     GEAPDDVYVLESGRLRVELVTPGATQMRVSTVLPGVIVGEVALYTQDRRTADVVAETP
                     SVVLRLSRTAIERMEAEEPQLAGLLHRWFAQTLAVRLSDRTRALDSLLD"
     gene            complement(2452..>4383)
                     /locus_tag="VF036_00925"
     CDS             complement(2452..>4383)
                     /locus_tag="VF036_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX6263822.1"
                     /translation="AVLMAIVSTSAVSHATGSPQDVFLSAIAAVMVATVVCGVVLLVM
                     GTRRRGDLIRFVPYPVVGGFLAGTGWLLVRGGLFAASGVSSYFTPLSDLLETSASQRL
                     LPAFAFGVILLVSVRVVKRPLVIPAVIAIGLVVFVAGMVATGSSIEELREGGWLLGPF
                     KASRLWEQPWTLSTLGGADWFSVLESWAGIVTAVFVATLAILFNISGSELVLDRDLDT
                     NQELRDAGVLNVVSGALGGIPGYHALSLTALAERMRVDARAAGLIAALVPLAAVVVGA
                     EVIGLIPRMIVGGVLVFLGLAFLVEWVWDKRRVLPPLEYAVVLAILAGIIIWGFLIGV
                     VIGLVLAVLLFAVSYGRIELVREVSFGETYHSNVDRPASERAELRGLTDRVQILRVSG
                     FVFFGSTNRLLEKVRHRVEEAPPRFLVIDLWRVTGVDSSAVVSFVKVLSLAEAHGFEV
                     IFTSASDPVRAQLARGGVEETRGLAVFEPDLDRGLQRCEDALLSSPSLDEAKAEHPAA
                     SGPDGLPPGLAAHLERVSVPAGTVLLHQNEPPGDIYELESGRLVVETSTHDGPRLRLR
                     TLRPGVVVGEVTLYTDVPRTADVVAEVPSVVLKIGRAEIERLEREQPELAATLHRWLA
                     TQLAERLTDTMRTFDVLLD"
BASE COUNT          808 a         1571 c         1458 g          546 t
ORIGIN      
        1 gacggcgacc ggctcaccct gttcaacgac ccgaactgct cccgggatcg gggaacgtac
       61 acgtgggagc tggagcgcgg atcgctgcac ctcgagccca tcgacgactc gtgcgcgtac
      121 gaaggggagc gggcgaccga cctcgcgacg gaggattgga cgcggatccc ggtgtgcagg
      181 cgcgagatcg cgcacctgtg gcccggcatc ctcggttgct gatctccgac ctcagtcgag
      241 gagggagtcg agtgcgcgtg tccggtcgct gagacggacc gccagcgtct gcgcgaacca
      301 acggtgaagc agccccgcga gctggggttc ctcggcttcc atccgctcga tcgccgttcg
      361 gctgagccgc aagacgacgg acggcgtctc ggcgacgacg tccgcggtac gccggtcctg
      421 cgtgtacaac gcgacctctc cgacgatcac tccgggcagc accgtgctca cgcgcatctg
      481 cgtcgccccc ggagtgacga gctccacgcg gagtcggccg gactccagca cgtagacgtc
      541 gtctggagct tcgccctgtt cgatcagctt cgtgccttcc tcgagactga cacgctcgaa
      601 gtactcccag agatgcggcg gcaggccctc caggaccggg acggttctct cgcccgggac
      661 gagatccatc agcagcgcgt cctcgcaccg ctccagcccc cggtccaggt cgggctcgaa
      721 ggacacgaga cccggtgtct cctctacgcc tccgcgggcc agctgtgcgc gcaccgggtc
      781 ggaggcgccg gtgaacacga cctcgaagcc ggaggcctcc gccaggcgga gcaccttcac
      841 gaacgagacg accgccgagg agtccacgcc ggtcacgcgc cgcagatcga tcagcaggaa
      901 ccgcggcggc gtgtcctctt ccacgcgccg tcggatccgc tccagcaggc ggttcgtcga
      961 gccgaagaac acgaacccgg acacccgcag gatctggacg cgatcggaga gcgtgcggag
     1021 ctcggcgcgc tcggccgccg gccgatcgac gttggagcgg taggtctcgc cgaaggagac
     1081 ctcacggacc agctcgatcc ggccgtagtt gatcgcgaac agcatcacgg cgagcaccag
     1141 cccgatcacg accccctcgc ggaaccccca cacgatgatc cccgccaaga tcgcgaccac
     1201 gacccagtac tcgagtcggg gcaggacttt ccgcttgtcc cagacccacc cgaccaggaa
     1261 gtcgagcccg atgaagatca gcacgccccc gacgatcatc ctcgggatca gcccaacgac
     1321 cgaagcggcg aaggcgacgg ccgccagtgg aaccgccgcc gcgatcagac ctgcggcccg
     1381 agcgtccacc ttcaggtacc gcggcaacgc cgtgagcgag agcgcgtggt agccggggat
     1441 cccgccgagc gcgcccgatg cgacgttcag gatcccggcg tcacggagct cctggttcgt
     1501 gtcgaggtcc cgatccaaga cgagctcgga gcccgagatg ttgaacagga tcgcgagcgt
     1561 ggccacgaac acggcgtcca ggatcgtgac ccacgattcg aggatcgacg accagtcggc
     1621 tccggcgagc gcttcagggg tcacgaactc ccacggcttc gaagtctgga acggcccgaa
     1681 cagcagccat ccatccgcgc ggacctcgtc gatcgattgg ccggtcacga tcatccagac
     1741 gacgaacgac acgagaccga tcgcgatcac cgccgggatc acgaacgggc gcttccagac
     1801 ccggaccgcg atcaagagga tcacgccgaa cgtcaacgcc gggagcagac ggctcagctg
     1861 gaactcgaac agctggagga cgttcccgag cttcacctgc atccccgtgg atgcgttgac
     1921 gccggccttg aacagcagcc accccgtgcc cgcgaggaac cctccgacga ccggatacgg
     1981 cacgaaccgg accaggctgc cgagtcggaa cctccccatc acgaagaaca cgatcccgca
     2041 gaggaccgtc accacgaacg tcgcggcgac gacggtgagg aagatgtcgg gtcgctcgaa
     2101 gtcctgcagt ccgctctgct gtgcgacgtg ctcgagctcc gcgaccttct tcgccaccgc
     2161 ggcggcgacg agcgagagca cggcggcggc ggcatcctgc acgctgccca cgacgccccg
     2221 gtgaccggcc cgccacgcga agaacgccag cgtcagggct gccgcgccga ggtacaggcc
     2281 tatgccctgg ggcaggtcat tcaccagggc gccgccgccg aacacgaagg ccgcgaacgc
     2341 gatggcgaga acgcactcga ccgccccgat gatcaggcca gcggcgaccc acgtgagcca
     2401 cgtgccggtc cgtcgccgtt cgaccatcgg tgtctccatc tccacctcgc gtcagtccag
     2461 gagaacgtcg aaggttcgca tcgtgtcggt cagccgctcg gcgagctgcg tggcgagcca
     2521 gcggtgcagc gtcgcggcga gctcgggctg ctcccgctcg agccgctcga tctcggcccg
     2581 gccgatcttg agcacgacgc tcgggacctc ggccacgacg tcggcggtgc gtgggacatc
     2641 ggtgtagagg gtcacctcgc cgaccacgac gcccgggcgg agcgtccgca ggcggagcct
     2701 cggcccgtcg tgcgtcgagg tctccacgac caggcggccg gattccagct cgtagatgtc
     2761 tcccggcggc tcgttctggt ggagcaggac cgtgccggcg ggcaccgaga cgcgctcgag
     2821 gtgggcggcg agacccggcg gcagcccgtc ggggccggag gcggctgggt gttccgcctt
     2881 ggcctcgtcc aggctcgggg acgagagcag cgcgtcctcg cagcgctgga ggccccgatc
     2941 caggtcgggc tcgaagacag cgagcccgcg ggtctcctcc acgcctcccc gggcgagctg
     3001 ggcgcgcacc ggatcggagg cgctggtgaa gatcacctcg aagccgtggg cctccgccag
     3061 ggagagcacc ttcacgaacg agacgaccgc cgaggagtcc acgcccgtca cgcgccacag
     3121 atcgatcacc aggaaccgcg gcggcgcttc ctccacccgg tggcggacct tctccagcag
     3181 gcggttcgtc gatccgaaga agacgaagcc cgagacccgc aggatctgga cccggtcggt
     3241 gagcccccgg agctcggcgc gctcggaggc cggtcggtcg acgttggagt ggtaggtctc
     3301 gccgaacgag acctcgcgga cgagctcgat ccggccgtag ctgacggcga acagcagcac
     3361 ggcgagcacg aggccgatca cgaccccgat caggaacccc cagatgatga tcccggccaa
     3421 gatcgcgagg acgacggcgt actcgagcgg cgggagcact cgccgcttgt cccagaccca
     3481 ctcgaccagg aaggcgagcc cgaggaacac caggacgccc ccgacgatca tccgcgggat
     3541 caacccgatc acctccgcgc cgaccacgac agcggccagg gggacgagcg ccgcgatcag
     3601 gcccgcggcc ctcgcgtcga cgcgcatgcg ctcagcgagc gccgtgaggg aaagggcgtg
     3661 atacccgggt atccccccga gcgcccccga gacgacgttc aggacgcccg cgtcgcggag
     3721 ctcctggttc gtgtcgaggt cccggtctaa gacgagctcg gagcccgaga tgttgaacag
     3781 gatcgcgagc gtggccacga agaccgcggt cacgatcccg gcccacgact cgagcacgga
     3841 gaaccagtcg gcgccgccga gcgtgctgag cgtccacggc tgctcccaca accggctcgc
     3901 cttgaacggt ccgagcagcc atccaccctc gcggagctcc tcgatcgagg agccggtggc
     3961 gaccatcccc gccacgaaaa ccacgagccc gatcgcgatc acggccggga tcacgagcgg
     4021 gcgcttcacg acccggaccg agacgagcag gatcacgccg aacgcgaagg cagggagcaa
     4081 gcgctgcgat gcggaggtct ccaagaggtc gctcaacgga gtgaagtacg atgacacccc
     4141 cgaggcggcg aacagaccac cccgcacgag cagccatccc gtgcccgcga ggaagcctcc
     4201 gacgactgga tacggcacga accggatgag atcaccccgc cggcgggtcc ccatcaccag
     4261 gaggacgacc ccacacacga ccgttgccac catgaccgcg gcgatcgcgc tgaggaagac
     4321 gtcttgcggg cttccggtgg catgcgagac ggcgctcgtc gagacgatcg ccatcagcac
     4381 cgc
//
LOCUS       DASUVE010000046         2644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1343559, whole genome shotgun sequence.
ACCESSION   DASUVE010000046 DASUVE010000000
VERSION     DASUVE010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343559"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VF036_00930"
     CDS             <1..711
                     /locus_tag="VF036_00930"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6263823.1"
                     /translation="DLRHVLPSIAAPTLVMRWQDDKNSEASSGRFLADHVQNERFVEL
                     PGGDQLSWTGDHDPVFEELLSFMGTEAPASQGTRQLATVLFTDIVDSTTRAAEMGDAA
                     WRDTLERHHGAVRACLSAHGGTEVDTAGDGFLATLDGPAAAVRCALEIVGSVKEVGLE
                     IRAGVHTGEVETIDGKVGGLGVHIGSRVSAAAGPSEVFVSSTVKDLVAGSDLVLEDTG
                     EHELKGVPDRWRLYRVVS"
     gene            711..2201
                     /locus_tag="VF036_00935"
     CDS             711..2201
                     /locus_tag="VF036_00935"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEX6263824.1"
                     /translation="MPSPEEPSRVEEAVRFLERISPFRELGPAQIEDVALHALVRSYP
                     TGTEILHQAAEPARYLFVIHRGAVELLDEGQLVDVLGEGDLFGISVFSKVGPALTARA
                     REDTECYLIESDRARAVMGTEAGLASLAAHIARWRERGAAEQHAHEIGVDDSLLRAIG
                     AAHDITELSTIVGGFPAVVAELLDRRSDAIAIGHVVGMSIDHMTIRLIELHVEDRGPP
                     PVPFAWVALGSAARHEQALNTDQDHAIAYGDDSDVGTIDTYFEGLAESVTEGLEACGI
                     ERCHGGVMAVNPAWRRTRERWRDRFREYATDPDLMGARITGIAFDYRRVTGPVDIESP
                     LDEVIREAGRDRAFVRRLATTALEASPPVGRRSDIELPRHGEHAGRIDVKHDGITIVT
                     NLARVYGIVAGVSENRTTERLRSAADAGVIDGTTTNDLMEAFRLLWRIRLERHSELAA
                     RGEPPDDFVDPSGLSRVTERALGGSLRVIGDAQAALAGQLGLRHRR"
     gene            2444..>2644
                     /locus_tag="VF036_00940"
     CDS             2444..>2644
                     /locus_tag="VF036_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263825.1"
                     /translation="MPAALALVVPFFGPLVAWLSPTVAVPRRDLAWPGSSRRTALLAA
                     LATPAVIWASLILFPNGAFLLVP"
BASE COUNT          399 a          892 c          950 g          403 t
ORIGIN      
        1 gacctgcgcc acgtgcttcc gtcgatcgcg gcgccgacac tcgtcatgcg ttggcaggat
       61 gacaagaact ctgaggcgtc gagcggccga ttcctggcag accacgtcca gaacgagcgg
      121 ttcgtcgagc tccccggcgg agaccaactc tcctggacgg gcgaccacga tcctgtcttc
      181 gaggagctcc tttcgttcat gggcacggag gcgccggcga gccaggggac gcggcagctc
      241 gcgaccgtcc tgttcaccga catcgtcgac tcgacgacga gggcggccga gatgggcgac
      301 gcggcgtggc gagacaccct cgagcgccat cacggagccg tccgcgcgtg cctgtcggcg
      361 cacggcggca ccgaagtgga cacggcaggc gacggattct tggcgaccct ggacgggccg
      421 gcggccgccg tccggtgcgc gctggagatc gtcggatcgg tcaaggaggt cggtctggag
      481 atccgcgccg gtgtccacac cggcgaggtc gagacgatcg acggcaaggt cggtgggctg
      541 ggcgtgcata tcggttcacg ggtgagcgcc gccgccggcc catccgaggt gttcgtgtcc
      601 tcgaccgtca aggacctcgt cgccgggagc gacctcgtcc tcgaggacac aggggagcac
      661 gagctcaagg gcgtccccga ccgctggcgc ctctaccgtg tggtctcctg atgccctcgc
      721 cggaggaacc ctcgcgcgtc gaagaggccg tccggttcct cgagcgcatc tcgccgttcc
      781 gcgaactggg gcccgctcag atcgaggacg tcgcgctcca cgcgctcgtc cgctcgtacc
      841 cgaccggcac ggagatcctc caccaggcgg cagagcctgc tcgctacctg ttcgtgatcc
      901 atcgtggcgc cgtggagctc ctcgacgagg ggcagctcgt ggacgtgctg ggcgaggggg
      961 acctcttcgg catctcggtg ttctccaagg tggggcccgc gctcacggcg agggcgcgtg
     1021 aggacacgga gtgctatttg atcgagtcgg atcgcgcccg cgcggtcatg gggacggagg
     1081 ccgggctcgc gtccctcgcc gcgcacatcg cgcgatggcg cgaacgcggc gccgcggaac
     1141 agcacgcgca cgagataggg gtcgacgact cgttgctccg ggcgatcgga gcggcgcacg
     1201 acatcacgga gctctcgacg atcgtcgggg gcttccccgc tgtcgtcgcg gaactcctcg
     1261 atcggcgctc cgacgcgatc gcgatcggtc acgtcgtcgg catgtcgatc gatcacatga
     1321 cgatccggct gatcgagctc cacgtcgagg accgcggccc tccgccggtc ccgttcgcgt
     1381 gggtggcgct gggcagcgcc gcgcggcacg agcaggcgct caacaccgat caggaccacg
     1441 cgatcgccta cggagacgac tccgacgtcg ggacgatcga tacgtatttc gagggcctgg
     1501 ccgagtcggt cacggaaggg ctcgaggcct gcgggatcga acggtgccac ggtggcgtga
     1561 tggcggtgaa cccggcatgg cggaggaccc gagaacgctg gcgcgaccgt ttccgcgagt
     1621 acgcgaccga tcctgacctg atgggagcgc ggatcacggg gatcgcgttc gactaccggc
     1681 gcgtgaccgg ccccgtcgac atcgagtccc cgctcgacga ggtgatccgc gaggccggca
     1741 gggaccgcgc gttcgttcgc cggctcgcga cgaccgcact cgaggcgagc ccgccggtcg
     1801 gcaggcggag cgacatcgag ctgccgcgcc acggcgaaca cgcgggccgg atcgacgtga
     1861 agcacgacgg catcacgatc gtgacgaacc tcgctcgcgt gtacgggatc gtggcgggtg
     1921 tgagcgagaa ccgaacgacg gagcggctcc gcagcgccgc cgacgccggt gtgatcgacg
     1981 gaaccactac gaacgacctg atggaggcgt tccgcctcct gtggcggatc cgactcgagc
     2041 gacactcgga actcgctgct cgcggagagc cgccggacga cttcgtggac ccgtcgggtc
     2101 tgtcccgcgt cacggagcga gcgctcggtg ggtcgctccg ggtcatcggc gatgcacagg
     2161 cggcgctcgc cgggcagctg ggtttacggc atcgacggtg ataggactcc tcggctccgc
     2221 cgcatcaggc gggagaggtg agatgcgagt tggagttccg cttgtgacct ccgggcatcc
     2281 agttcaccat cggaggtcga aagggggcag ttgatgcaag gtcggcacca cgagccggtc
     2341 gtcagctgag atgggcgacg caggagagcc cgcccctcgc aggcccgacg tacctccacc
     2401 gccacccggg gcaggaaggc cactggcccg cgcggtcccc ttcgtgcccg ccgcgctcgc
     2461 gctcgtcgtg ccgttcttcg gcccactcgt cgcctggctg tcaccgacgg tcgcggttcc
     2521 gaggcgcgac ctcgcgtggc cgggatcgag tcgccgaaca gccttgctgg cagctctggc
     2581 cacgccggcg gtgatctggg cttcgttgat cctgttcccc aacggcgcgt tcctgttggt
     2641 tccg
//
LOCUS       DASUVE010000047         7996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1343953, whole genome shotgun sequence.
ACCESSION   DASUVE010000047 DASUVE010000000
VERSION     DASUVE010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343953"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            693..2363
                     /locus_tag="VF036_00945"
     CDS             693..2363
                     /locus_tag="VF036_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6263826.1"
                     /translation="MSIEHTTSLDEANAALAAGEWEQARAGFERALVDEPKPGAEEGL
                     ARALWWLRDPEQGIAHMERAYAGFREAGNVSRAARAALWLAREYQAVRGNTAASNGWV
                     ARAEGLLRDAAPASEHGWLALTRAERAGDLGEMGRQAEEAHRIAVATGDRDLEAAALA
                     RRGYAELAAGGVASGTDRLDEAMAAATGGDVRSLDVVGDITCVAIAAFELSSDWQRIE
                     QWGQVMESWIQNHDDVAVLGFCYACCSEIFLASGQWDEAEGMLTQGLGALRAAGHRSR
                     CVHPAAKLAELRLTQGRLEEADQLLAGFEELPEAAHGIAHLHLVRGEGALAAATIHRR
                     LNRIGDDNVLAAPFLAMLVEVQLSQDDVDGAERSADRLGSIAHASALDRVTAMADLAR
                     GRVAAARGSDDALDALEAALARFARVGMSVEAARARLELARALEADQPEVAVAEARTA
                     LAEFERVGAPRDADAAAELLRRHGVKGRTGPKDAGLLTRREQEVLHLLAGGLSNAEIA
                     ARLHLSTKTVGHHVSSVLAKLSVKSRGEAAAWVGRHLRAPDPEDPAER"
     gene            2425..2973
                     /locus_tag="VF036_00950"
     CDS             2425..2973
                     /locus_tag="VF036_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6263827.1"
                     /translation="MQRTGERGANALKQELVVHVEATSGASAEHVYAVLADLSTHTVW
                     AGERQGTKTRLLTIDAPAGPATVGTEFSSTGSDPMGSFADRSVVTEAKPGRTFEFVTE
                     ARFSTRKGATSDWTNVERYELEPLADGCRIVCTGRVARMSSLPGTLKVFNVPGLRGLA
                     LKASARVSRRTVRNLARYAEER"
     gene            3034..3405
                     /locus_tag="VF036_00955"
     CDS             3034..3405
                     /locus_tag="VF036_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6263828.1"
                     /translation="MGTVSKKSTGEPITGPGFEGSYGETEGYTVGFERYTEHADLAPY
                     FVGLPEDRCQSPHWGYVFSGKLVFHTAKGPVEITDGMAYYVGPGHTPEIFPGTELVEF
                     SPTADFQETMDVVGKNMESMG"
     gene            3540..4559
                     /locus_tag="VF036_00960"
     CDS             3540..4559
                     /locus_tag="VF036_00960"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX6263829.1"
                     /translation="MEQMNEGAVFAQTNAAHNEVIAYSRDADGMLTQTGTFATGGAGS
                     DEAHLPSQGSVTLTKDRRHLLVTNVASNDVSVFSLDGAMPALTKTIPTGAAPRSVAEH
                     DGLVFVLATGDARIDGFRLMDGDLVPVEGASKALSTPDGAQIGFTPDGRALIATERGG
                     DKLSAFAVGMDGMLGEPRTIDSLGATPYGFAISSTGTLVVTEAFRAEKGAAAASSYRV
                     DGDTITPGTASVANGRSEICWAVISNDGRYAFTTNFADGAVSRYAIDMDGGLTLEDAT
                     AGVTEDGRAGLRDEDLSDDGRFLYAIDADQGQIVGWSVDERGHLSMLGAWDGVPTTVA
                     GLAAR"
     gene            4559..5002
                     /locus_tag="VF036_00965"
     CDS             4559..5002
                     /locus_tag="VF036_00965"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 family protein"
                     /protein_id="HEX6263830.1"
                     /translation="MRIEPLYRVTFTTPEAWNVTREGGSGTEAQSFLIAEGRAEGRLS
                     ARYRAANFPRGRVDGALEPEFRGVLETDDCATILFHWEGLATLTDTGMRSLLGMAQHT
                     SEDERYRWLNDRVCTVEGEVRPRTESTGFDVVLSVSEMIWEPVSG"
     gene            complement(5062..5304)
                     /locus_tag="VF036_00970"
     CDS             complement(5062..5304)
                     /locus_tag="VF036_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6263831.1"
                     /translation="MSAQGLTCHEMVEIITDYLEEALQPEDRRRVEEHLAICDGCTTY
                     LEQMRETIRLTGMLSEEQIPEEHKRQLLDAFREWRR"
     gene            complement(5301..5954)
                     /locus_tag="VF036_00975"
     CDS             complement(5301..5954)
                     /locus_tag="VF036_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6263832.1"
                     /translation="MRDTGVLASPADLAIVQRLRAGDEATFMMLVEQHQAEMLRIARM
                     YLSSRAVAEEAVQEAWLGIIKGLDRFEGRSSLRTWMYRIVANVAKTRGQREGRSVPFS
                     SLAGDDAEPAIDAEWFQSATDRAPGGWRTFPDDWRGIPEDRLIGNETMQRIGIAIDLL
                     PPMQAEVLRLRDVLGWTSQEVRNALDLSETNQRVLLHRARSRVRRELDTYLSEGRDR"
     gene            complement(6021..6599)
                     /locus_tag="VF036_00980"
     CDS             complement(6021..6599)
                     /locus_tag="VF036_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263833.1"
                     /translation="MDGYGPVLRALMTFDPAVHVFGGFAEDALLHGRCVRTHDDVDVL
                     VGREELEAQLRNAHGIGFASHEVRFEPIEGMPMVIGTTDGSRDLEISVYDRSPEGSVF
                     FHIPNQEGRLFRIDLSNGVFDHPLSDLDDITLRTVSPLALYQIRAGITMAGGFGPPRR
                     KDVTSQEALRARFFPDASPESLQPRMTDVSPG"
     gene            complement(6957..7463)
                     /locus_tag="VF036_00985"
     CDS             complement(6957..7463)
                     /locus_tag="VF036_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263834.1"
                     /translation="MDEIGPATNDRAGFGESARSHRVERLLIVHPQDDADVGAAASEL
                     KDLPGVVGVTLNTATGAFDILLDPVVVSDDELAAALHHQGYDLTSWQEVRAMHAVRQR
                     SWLLEQIRSLASRAELELAERGAYANGVTKGSIDAYIRTARAFGLVTDAEIVQLIPRG
                     SLSEGGAG"
     gene            complement(7497..>7996)
                     /locus_tag="VF036_00990"
     CDS             complement(7497..>7996)
                     /locus_tag="VF036_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020511967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6263835.1"
                     /translation="WALGVEARSRALLTEGDAAEKLYRIAIERLSGTRVRFELARAHL
                     VYGEWLRRENRRVDAREQLRTAHDAFSSMGADAFADRARRELVATGETVRSRVDETRD
                     VLTPQEAQIARLARDGFSNPDIGARLFISPRTVQYHLHKVFLKLDISSRNQLGRVPPS
                     RLGAA"
BASE COUNT         1335 a         2581 c         2807 g         1273 t
ORIGIN      
        1 aacctgcgac acacggtcct aggaaccgtg gctcgagatt cgcgtgtccg ctctccggca
       61 gtctcgcacc tgggtgaaac gtcaggcggc tcaagtcgtc gtgttggtgt tgctcgcatt
      121 cctgggcggg gtcgtcgctc agtcgatcgc accgcctggg gcgggagact caggtcaggt
      181 gtccccctcg tttcacttgg tctagctcgc ccggcgaccg gtcggcgggg aagggagcat
      241 ggcagcgagg ctgagcatcg ggcctatcga agatcggtct gagacacccc cttttggcgc
      301 tgacgcaagg agggcgtgag atcgatcgcc gtgggatcta gtcaacacca ggtcaaggag
      361 ttcgaccgaa ccaatcggta ccagacggaa ctcctgagac agctcagagc tggcctgagc
      421 tgcaaagacg ctggtcaggg ccctgcgtcg gccttctttg acacggagga ggtcagaggt
      481 tcaaatcctc tagcgcccac caagaagccg caggtcagaa gacctgcggc tctcttcatc
      541 cgtatcgatt ctgggaccga ggtggcaatt aggtggcacg ccgggcgtcg tctggccgtc
      601 cgtgcaacag tcaacgcctg gtcaacgagc aagaagccag ctccacccct cagcccttct
      661 cgcatcgagt cctcggtgcg atgattcgcg ccgtgtcgat cgagcacacg acctcgttgg
      721 acgaggctaa cgcagcgctc gccgccggcg aatgggaaca ggcgcgggcc ggcttcgagc
      781 gggccctggt ggacgagccc aaacctggcg cggaggaggg tctggcgcgc gccctgtggt
      841 ggctgcgaga tccagagcag gggatcgccc acatggagcg cgcgtacgcg ggcttccgcg
      901 aggccggcaa cgtgtccagg gccgcgcgcg cagcgctgtg gctcgcccgg gagtaccagg
      961 ccgtccgcgg gaacacggcg gcgagcaacg gctgggtcgc gcgggccgaa gggctcctcc
     1021 gggacgccgc cccagcctcc gagcatggct ggctcgcgct cacccgggcc gagcgtgccg
     1081 gcgatctcgg tgagatgggc cgccaggccg aggaagcgca ccggattgcc gttgccaccg
     1141 gcgatcggga cctcgaggcg gccgcgctcg cccgacgagg gtacgcggaa ctcgccgctg
     1201 gcggcgtcgc gtcgggcacg gatcggctcg acgaggcgat ggccgcggcc acaggcggcg
     1261 acgtgcggag cctcgacgtc gtcggcgaca tcacgtgcgt cgcgatcgcg gccttcgaac
     1321 tctcgtctga ttggcagcgc atcgagcagt gggggcaggt gatggagtcg tggatccaga
     1381 accacgacga cgtcgccgtc ctcgggttct gttacgcgtg ctgctccgag atcttcctcg
     1441 catcgggaca gtgggacgag gccgaaggga tgctcaccca gggcctcggg gcgctccggg
     1501 cggccggaca tcgatcgaga tgcgtccatc cggcggccaa gctcgccgag ctgcgcctca
     1561 cacagggtcg gctcgaggaa gccgaccagc tcctggccgg gttcgaggag ctgccggagg
     1621 ccgcccacgg gatcgcgcac ctgcacctag tccgaggaga aggggcactc gccgctgcga
     1681 ctatccatag acgcctgaat cggatcgggg acgacaatgt cctcgcggcg ccgttcctcg
     1741 cgatgctggt cgaggtgcag ctgtcccaag acgacgtcga cggcgcggag aggtcggcgg
     1801 atcgcttggg gagcatcgca cacgcatcgg cgctcgatcg cgtgaccgcg atggcggatc
     1861 tggcgcgcgg gcgggtggcg gccgcgcgtg ggagcgacga cgccctcgac gccctcgagg
     1921 ccgcgctcgc gcggttcgcc cgtgtgggga tgtcggtcga ggcggcacga gcccggctcg
     1981 agctcgcccg tgccctggag gcggatcagc cggaggtggc cgtggccgag gcacgaaccg
     2041 cactcgccga gttcgagcgg gtcggggcgc cccgcgacgc cgacgcggcc gcggagctgt
     2101 tgcgccgaca cggtgtgaaa ggacggaccg gacccaagga cgccggactc cttacacgtc
     2161 gcgagcagga ggtgctccac ctcctggctg ggggtttgag caacgccgag atcgccgccc
     2221 gcctgcatct gagcacgaag accgttgggc accacgtgag tagcgtgctg gcaaagctca
     2281 gcgtgaagag tcgcggggag gccgccgcct gggtcggccg ccacctgcgc gcccccgacc
     2341 cagaggatcc cgccgagaga tagggaacat tcccgttcct cccaggacca tgtgagcgca
     2401 ccctccttcg tggaggtgat gcacatgcag aggacagggg aacggggcgc caacgccctg
     2461 aagcaggagc tggtcgtgca tgtcgaggcg acgtctggcg cctccgccga acacgtctac
     2521 gccgtgctcg ccgacctctc gacccacacc gtgtgggcgg gggagcggca gggcacgaag
     2581 acgcgcctgc tgacgatcga cgcgcccgcg gggccggcga ccgtcgggac ggagttctcc
     2641 agcacaggct ccgatccgat gggctcgttc gcggatcgct ccgtggtcac ggaggccaag
     2701 ccggggcgga cgttcgagtt cgtcacggag gcccgattct cgacgaggaa gggggcgacg
     2761 agcgactgga cgaatgtcga gcgctacgag ctcgaaccgc tcgccgacgg ctgccggatc
     2821 gtgtgcaccg gtcgcgtcgc caggatgagc tcgctccccg ggacgctcaa ggtcttcaac
     2881 gtcccggggc tgcgcggcct ggcgctcaag gcttcggcgc gggtctcccg gcgcacggtc
     2941 cgaaacctgg cgcgctacgc ggaggaacgc tgaaacgaac gtcaagctcc gggagacgga
     3001 gcggcgaccg cacgatggga aagggggcat ctgatgggaa cggtgtcgaa gaagagcacg
     3061 ggtgagccga tcacaggccc aggcttcgag gggagctacg gcgagacgga gggctacacg
     3121 gtggggttcg agcggtacac ggagcacgca gacctcgccc cctacttcgt ggggctgccg
     3181 gaggatcggt gccagagccc gcactgggga tacgtcttct cagggaagct cgtgttccat
     3241 acggcgaagg gccccgtcga gatcaccgat gggatggcct actacgtggg tccgggtcac
     3301 acgccggaga tcttccccgg cacggagctc gtcgaattca gcccgacggc cgacttccag
     3361 gagacaatgg acgtcgtggg gaagaatatg gagtcgatgg gctagcccgt cgacgctacg
     3421 cgagccacgc gggtcggacc agttgggtcc gacccgcatc acgttccggg gaaccgtgta
     3481 acgggacggc ggtccaggag cacaggaacg cgtcggaagc acacggaaag ggggccggga
     3541 tggaacagat gaacgagggc gctgtgtttg cgcagacgaa cgcggcgcac aacgaggtga
     3601 tcgcatactc ccgcgatgcc gatggcatgc tcacgcagac ggggaccttc gcgacgggtg
     3661 gcgccggcag cgatgaggcg catctgcctt cccaaggctc ggtcacgctg accaaggatc
     3721 gccgtcatct gctcgtaacg aatgtcgcca gcaacgacgt atccgtgttc tcgctcgatg
     3781 gggcgatgcc ggcgctgacg aagacgatcc cgacgggtgc ggctccacgc agtgtcgcgg
     3841 agcacgatgg gctcgtgttc gtgctcgcaa cgggcgacgc gcggatcgat ggtttccgcc
     3901 tgatggacgg cgatctcgtg ccggtcgaag gggcgtcgaa ggcgctgtcg acccccgatg
     3961 gcgcgcagat cggcttcacg cccgacggtc gcgccctcat cgccaccgag cgcggcggcg
     4021 acaagctctc cgctttcgcg gtcggcatgg acgggatgct cggggagcca cgcacgattg
     4081 actcgctcgg cgcgactccg tacgggttcg cgatctcgtc gacaggaacg ctcgtcgtga
     4141 ccgaggcctt ccgcgctgag aagggtgcgg ccgcggcgtc gagctaccgc gtggacggcg
     4201 acacgatcac gcccggtacc gcgagcgtgg ctaacggtcg cagcgagatc tgctgggccg
     4261 tgatctcgaa cgacgggcgc tacgccttca ccaccaactt cgcggacggc gccgtgtcgc
     4321 gctacgcgat cgacatggat ggcgggctca cactcgagga cgccaccgct ggcgtcacgg
     4381 aggacggtcg tgctggactc cgtgacgagg acctgtcgga tgacggtcgg tttctctacg
     4441 cgatcgacgc cgaccagggg cagatcgttg gttggtcggt cgacgagcgc ggacatctct
     4501 cgatgctcgg cgcctgggat ggtgtgccga cgacggttgc ggggctggcg gcgcgctgat
     4561 gcggatcgaa cccctctacc gggtgacgtt caccacgccc gaggcgtgga acgtcacccg
     4621 ggaaggcggg tccggtaccg aggcacagag tttcctgatc gccgaggggc gggccgaggg
     4681 ccgcctctcg gcgcgctacc gtgccgcgaa cttcccccgg gggcgggtcg acggcgcgct
     4741 cgagcccgag ttccgcgggg tcttggagac ggatgactgc gcgacgatcc tgttccattg
     4801 ggagggactc gcgaccctga ccgatacggg catgcgaagc ctcctcggga tggcgcagca
     4861 cacgagcgag gacgagcgct accgatggct caacgatcgg gtgtgcacgg ttgagggcga
     4921 ggtccgaccg cggaccgaga gcaccggctt cgatgtcgtg ctgtcggtga gcgagatgat
     4981 ctgggagccg gtctcgggtt gaccggggga gccgcctgac gagctctctt cagtcggcga
     5041 gagcgaacgt ccgttccccg gtcagcgcct ccactcgcgg aacgcgtcga ggagctgccg
     5101 cttgtgttcc tcggggatct gctcctcgct cagcatgccg gtgagccgga tggtctcgcg
     5161 catctgctcg aggtaggtcg tgcatccgtc gcagatcgcg aggtgctcct cgacccggcg
     5221 gcggtcctcg ggttgcagcg cctcctcgag gtagtcggtg atgatctcga ccatctcgtg
     5281 gcacgtcagc ccctgcgcgc tcatcggtcc cgtccttccg acaggtaggt gtcgagctct
     5341 cggcgaacgc gggaacgcgc gcgatgcagg agcacccgtt ggttggtctc cgacaggtcg
     5401 agcgcgttac ggacctcctg cgaggtccag cctaggacgt cgcgcaaacg gagcacctcg
     5461 gcttgcatcg gagggagcag gtcgatggca atgccgatcc gctgcatcgt ctcgttgccg
     5521 atcagccggt cctcggggat gccgcgccag tcgtcgggga acgtccgcca gccccctggg
     5581 gcgcgatcgg tcgccgactg gaaccactcg gcgtcgatcg cgggctcggc atcgtctccc
     5641 gccagcgagg agaacggcac gctgcggccc tctcgctggc cgcgcgtctt ggccacgttc
     5701 gcgacgatcc ggtacatcca cgtccggagc gacgatcggc cctcgaaccg atccaggccc
     5761 ttgatgatgc cgagccacgc ctcctgtacg gcctcttcgg cgacggctct cgaggagagg
     5821 tacattcgtg cgatccggag catctcggcc tgatgttgct cgacgagcat catgaacgtc
     5881 gcctcgtcgc cagcgcgcag ccgctggacg atggcgaggt ctgcggggga ggcgaggacg
     5941 ccagtgtcac gcactcggcc aatcctcccg gtggtgagaa agccgcatgt gtcggactga
     6001 gtgcactctt gtcgcacccg tcatccgggc gacacgtccg tcatcctcgg ttggaggctc
     6061 tcgggcgagg catccgggaa gaacctggcg cgaagcgcct cctgcgaggt gacatccttc
     6121 cgacgaggtg ggccgaaacc tcccgccatc gtgatccccg cgcggatctg atacagggct
     6181 aacggggata ccgtgcgcag ggtgatgtcg tcgagatccg atagggggtg gtcgaagaca
     6241 ccgttcgaga ggtcgatacg aaagagtctc ccctcctgat tcgggatatg gaagaacacg
     6301 gagccttctg gcgatcggtc atacacgctg atctcgaggt ccctgctccc gtcggtggtc
     6361 ccgatgacca tgggcatccc ttcgattggc tcgaatcgga cctcatgtga tgcgaacccg
     6421 atcccgtggg cgttccggag ctgcgcttcc agctcctccc tgcccaccag gacatcgacg
     6481 tcgtcgtgtg ttctcacgca ccttccgtgg agcagcgcgt cctccgcgaa tccgccgaat
     6541 acgtgcacgg ctgggtcgaa cgtcatgagg gcccggagca cgggaccgta gccatccatc
     6601 ggacggcatc cttcatcatg tgtggccttc caagcaagac atgtgagcga gcagctcagt
     6661 gtgttcagtg ctctgaggac tgtccgtgaa tggttgcgca gtccggcctg gaccttccct
     6721 ttcgctagac gcgaatcggt caacaccagg tcaaggagcc gaccgaacca agcggcatta
     6781 aacggaactc ctgagcaagc ccaaacccgg cctgagctgc gaacacgctg gtcagagcag
     6841 tgggcgggcc ttccttgaca cggaagaggc cggaggttca aatcctccag cgcccaccaa
     6901 caacccagct gagattgcct cttgatccat gggcaccgcg tgccagattg ctccgcctaa
     6961 cccgcccctc cctctgagag cgaaccacga gggatcagct ggacgatctc ggcgtcggtc
     7021 acgagaccga acgccctagc ggttcggatg taggcgtcga tcgaaccctt cgtgacacca
     7081 ttcgcgtagg ctccacgttc ggcgagctcg agctcggcgc gcgaagcgag cgaccggatc
     7141 tgttcgagca gccagctacg ctggcgaact gcgtgcatcg ctcgcacctc ctgccagctg
     7201 gtgaggtcgt agccctgatg gtgcaaggcg gcagcgagct cgtcgtccga gacgactacg
     7261 ggatcgagga ggatgtcgaa agcccccgtg gcggtgttga gggtcacgcc gacgacgccg
     7321 ggcaggtcct tgagctccga ggcggccgcc ccgacatcgg cgtcgtcctg tggatgaacg
     7381 atcagcaggc gctccacacg gtgggagcgg gccgattcgc cgaacccagc tcgatcgttc
     7441 gtggccggac cgatctcatc catgatcgac tcctctcctg cgatgcccac tccctcctat
     7501 gccgcgccga gacggctcgg gggaacgcga ccgagctggt tccgggagct tatgtcgagc
     7561 ttgaggaaga ccttgtggag gtggtactgg acggttcggg ggctgatgaa cagcctcgcg
     7621 ccgatatcgg ggttggagaa cccgtcccgt gcgagccgag cgatctgtgc ctcctgaggt
     7681 gtgaggacgt cgcgggtctc gtcgacgcgg ctgcggacgg tctccccggt ggccaccaac
     7741 tcgcgacggg cgcgatccgc aaacgcgtca gcgcccatac tgctgaacgc atcgtgggcg
     7801 gtgcgaagtt gctcgcgcgc gtccacacgc cggttctcac gacgcagcca ctcgccgtac
     7861 acgaggtggg cgcgggcgag ctcgaagcgg acccgcgtcc cgctgagacg ctcgatcgcg
     7921 atgcggtaca gcttctccgc tgcgtcgccc tcggtgagga gcgcccgcga gcgcgcctcc
     7981 acaccgagcg cccagt
//
LOCUS       DASUVE010000048         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_967135, whole genome shotgun sequence.
ACCESSION   DASUVE010000048 DASUVE010000000
VERSION     DASUVE010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967135"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VF036_00995"
     CDS             <1..386
                     /locus_tag="VF036_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX6263836.1"
                     /translation="AAWCGDRVRVIGVEPEGCASMTAALAAGAPVTVPVGGLASDSLG
                     AARVGDHTFPIVRDRVDRMALVTEDQIRDAQRALWDDVRLIVEPGGAAAFAALRSGAI
                     DVSSGDRVCVVLSGGNCDPSTVTPT"
     gene            complement(399..953)
                     /locus_tag="VF036_01000"
     CDS             complement(399..953)
                     /locus_tag="VF036_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX6263837.1"
                     /translation="MPRYVALLRGINVGGKNPIPMPALKACFEDAGFEEVGTYIQSGN
                     VVFTGPSSNQAALTDRVEWTIRNAFAHYEASVVVKSRSQMRAVVDTAPKGFGKEPAKY
                     RYDVCFLKPSVRAKHVVASLPTKEGVDRIWAGSGVVYFSRLTSRATSSRMNRVASLPV
                     YKQMTIRNWNTTTKLRELLDAETA"
     gene            complement(966..1946)
                     /gene="hemB"
                     /locus_tag="VF036_01005"
     CDS             complement(966..1946)
                     /gene="hemB"
                     /locus_tag="VF036_01005"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013155365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX6263838.1"
                     /translation="MSFPRSRPRRMRRTPALRSLIRESDVRPSDLIAPMFVKEELSEP
                     VPIASMPGQSQHTLESLRKEAVEIAERGVLAFILFAVPAKKDAEGSEAWNPEGIGQQA
                     IAALRAEFGDDAVVIGDLCLDEYTDHGHCGVLNDLGEVDNDATVELYQRIALAQAEAG
                     AHLVGPSGMMDGQVAAIRSALDASEHHDVGILAYAAKFASALYGPFREAAEGAPRFGD
                     RTGYQEDPANLEEALREIRTDIDEGADIVMVKPALPYLDVIRRAKDETRFPMAAYHVS
                     GEYAMVKAAAANGWLDERRVVLELLTSIRRAGADLILTYHAKDAADWLRA"
     gene            1989..>2203
                     /locus_tag="VF036_01010"
     CDS             1989..>2203
                     /locus_tag="VF036_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX6263839.1"
                     /translation="MEPKKRWDGYVSFSYLEPGVDYKEFELSPQIGRVEPFVYPLTDE
                     QNERADRLLRDCVCISLQDHCGITPVD"
BASE COUNT          308 a          762 c          747 g          386 t
ORIGIN      
        1 tcgcggcatg gtgcggcgac cgggttcggg tgatcggtgt ggaacccgag ggctgcgcct
       61 ccatgacggc cgcgctcgcg gccggcgcac ctgtcacggt cccggtgggc ggcctcgcgt
      121 cggactcact cggcgcggcc cgggtcggcg atcacacgtt cccgatcgtg cgcgatcgcg
      181 tcgacaggat ggccctcgtc acggaggatc agatccgcga cgcacagcgg gcgctgtggg
      241 acgacgtccg gctgatcgtc gagccgggtg gggcggcggc attcgcggcc ctgcgatccg
      301 gagctatcga cgtctcctcg ggcgatcgtg tgtgcgtggt gctttccggc gggaactgcg
      361 atccctcgac cgtgaccccg acgtgatcct acggaacctc aggcggtctc cgcgtccagg
      421 agctctcgca gcttcgtcgt cgtgttccag ttccggatcg tcatctgctt gtagaccggc
      481 agtgaggcca cccgattcat gcggctcgag gttgctcggc tcgtgagcct cgagaagtag
      541 acgacgccgc tgccggccca gatccggtcc acgccctcct tcgtcgggag gctggccacc
      601 acgtgcttcg ccctgacgga cggcttcagg aagcacacgt cgtagcggta cttcgccggc
      661 tccttgccga atcccttcgg cgctgtgtcg acgaccgcgc gcatctggct ccggctcttc
      721 acgacgacgc tcgcctcgta gtgagcgaac gcgttccgga tcgtccactc gacccggtcg
      781 gtcagagcgg cctgattcga ggacggaccg gtgaacacga cgttgccgct ctggatgtag
      841 gtcccgacct cctcgaagcc ggcgtcctcg aagcatgcct tcagcgccgg catcgggatc
      901 ggattcttcc cgccgacgtt gatcccgcgg agcagcgcga cgtagcgagg catctggctc
      961 ggggctcagg cgcggagcca gtccgcggcg tccttcgcgt gataggtgag gatcaggtcg
     1021 gcgccggctc gcctgatcga cgtcagcagc tcgagcacga cgcgccgctc gtcgagccag
     1081 ccgttggcgg ccgccgcctt caccatcgcg tattcgccgc tcacgtggta cgcagccatc
     1141 gggaacctcg tctcgtcctt cgcgcgccgg atcacatcga ggtacggcag cgccggcttc
     1201 accatcacga tgtcggcgcc ctcgtcgatg tccgtgcgga tctcccggag cgcctcctcc
     1261 aggttcgcgg gatcctcctg gtagcccgtc cggtctccga accggggcgc accctcggcc
     1321 gcctcgcgga acggcccgta cagcgccgag gcgaacttcg cggcgtacgc gaggatcccg
     1381 acgtcgtggt gctcgctcgc gtcgagggcc gatctgatcg ccgcgacctg cccgtccatc
     1441 atcccgctgg ggccgacgag gtgggcgccg gcctcggcct gcgcgagcgc gatccgctgg
     1501 tagagctcga cggtggcgtc gttgtccact tcaccgaggt cgttgagcac gccgcagtgc
     1561 ccgtggtcgg tgtactcgtc gaggcagagg tcgccgatca cgaccgcgtc gtcgccgaac
     1621 tcggcccgga gtgccgcgat cgcctgctga ccgatccctt cggggttcca ggcttccgat
     1681 ccctccgcgt ccttcttcgc cgggacggcg aagaggatga acgcgaggac gccgcgctcc
     1741 gcgatctcga cggcctcctt gcggagcgat tcgagcgtgt gctgcgattg tcccggcatc
     1801 gacgcgatcg ggacgggctc gctgagctcc tccttcacga acatcggggc gatcaggtcg
     1861 ctcggccgca cgtcgctctc gcggatcaag gatcggagcg ctggcgtgcg gcgcatgcga
     1921 cgtgggcggg atcgcgggaa gctcacgagc cagagcgtac ctcgacggag atcgcggaag
     1981 gatgcctgat ggagccgaag aagcgctggg acggctacgt ctcgttctcg tacctggagc
     2041 cgggcgtcga ctacaaggag ttcgagctct cgccacagat cggtcgcgtg gagccgttcg
     2101 tctatccgct caccgacgag cagaacgagc gagccgaccg cttgctccgc gactgcgtat
     2161 gcatctcgct ccaggaccac tgcggcatca ccccggtgga cat
//
LOCUS       DASUVE010000049         3882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_967257, whole genome shotgun sequence.
ACCESSION   DASUVE010000049 DASUVE010000000
VERSION     DASUVE010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967257"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="VF036_01015"
     CDS             complement(<1..1319)
                     /locus_tag="VF036_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6263840.1"
                     /translation="MATRTDGSGVGGALKRLLVGRALSTSAQEHQLLPKTLALPVFSS
                     DPLSSVAYATEEMMLVLVTAGVAALSYRLPIALAIAALLAIVVTSYRQTVRAYPRGGG
                     SYIVARENLGTVPGLVAAAAILTDYILTVAVSVTAGTVAITSAAPSMVEHKVLLASLL
                     VAFVTAANLRGVKEAGAFFALPTYGFVAVVGLTIVLGFWQCLSGCHVADTSSIPIEPI
                     TAVTLFLLLRAFASGATALTGVEAIADGVQAFRRPQAKNAAATLAIMGVISISLFLGI
                     TVLAGLLDVRITEEIAHSRSVLSLIGEAILGDGLWFIVLQIFTAGILILAANTAFQDF
                     PRLSSILARDQFMPSQFRNRGDRLVFSNGVIVLAGLAIFLIWLYDASLTDLIQLYVIG
                     VFTAFTLSQAGMVRRWFRLRGEGWRWRATLNGVGAAVTGIVLVVATIT"
     gene            complement(1329..2027)
                     /locus_tag="VF036_01020"
     CDS             complement(1329..2027)
                     /locus_tag="VF036_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6263841.1"
                     /translation="MNRVLVVDDEPQIRRALRTSLEAHGYEVQTVGTGEEAVIGAAES
                     APELMLLDLGLPDVDGTEVIRRVRGFTELPIIVLSVRDAQQDKVAALDAGADDYVTKP
                     FAMEELLARARAAMRRAQPGEPPAPTMRFGTLEMDLGRRLVILDGETLRLTPTEYQLL
                     EAFATHPGKLLTHQWLLRKVWGQGYGTETTYLRTYVRALRKKLGDDAQAPQLIVTEPG
                     VGYRWIAESTDAES"
     gene            complement(2024..3466)
                     /locus_tag="VF036_01025"
     CDS             complement(2024..3466)
                     /locus_tag="VF036_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6263842.1"
                     /translation="MSEEHEAEGTAKLTLRGTVIGLITPAIAAAIAFPLAPARPEISI
                     ALFMLAVVIAAVAGGLGAGLIAGILASTIRPLVVEDPQLTFGFDDVEDVATGVVFLSV
                     ALIVGLLVGQASEDRARASRREREARFLGTLSSTLHTREVPDRVLHEFAEVLLEPFAL
                     SKVEVEATLDGTELRAVSRTDGETSSGPRTTVPLLAGEASIGRLVAERPEGGRPFSRD
                     DQLLLEAAARQATAALDRARLDARARIAQLDAETNQLRAAMFSSVTHDLRTPLASIKA
                     GVTSLLDAAVHHDPEQERELLTTILEETDRLNRLVGNLLDLARIRAGALTPTRQQAAM
                     DEIVEVVLARMRPRLARHTVAADLPSDLPDVSVDPVQLDQVLTNLLENAGRHAPEGSE
                     IRVGVQWIRDAVQVRIADQGPGVPSDERERVFEAFYRGSSSPETPGSGLGLAISKAVV
                     VAHGGRIWVESGPDGGCLVAFEIPREEVAP"
     gene            3778..>3882
                     /locus_tag="VF036_01030"
     CDS             3778..>3882
                     /locus_tag="VF036_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX6263843.1"
                     /translation="MGCGRVGSELANAIADEHDVAIIDKNPEAFHAYPP"
BASE COUNT          630 a         1364 c         1327 g          561 t
ORIGIN      
        1 gtgatcgtgg cgaccacgag gacgatcccc gtgacggcgg cgcccactcc gttcagggtg
       61 gcccgccagc gccagccctc cccccggagc cggaaccagc gtcggaccat cccggcctgc
      121 gagagcgtga acgccgtgaa gaccccgatc acgtagagct ggatcaggtc cgtgagcgag
      181 gcgtcgtaga gccagatcag gaagatcgcg aggccggcga ggacgatcac gccgttcgag
      241 aacacgaggc ggtcgccccg gttgcggaac tggctcggca tgaactgatc gcgagcgagg
      301 atcgacgaga ggcgggggaa gtcctggaac gcggtgttgg cggcgaggat caagatgccc
      361 gccgtgaaga tctgcaggac gatgaaccac aggccgtcgc cgaggatcgc ctcgccgatc
      421 agcgacagca cggagcgcga gtgcgcgatc tcttccgtga tccgcacgtc caagaggccg
      481 gcgaggaccg tgatcccgag gaagagggag atcgagatca cgcccatgat cgcgagcgtg
      541 gccgcagcgt tcttcgcctg cggccgacgg aacgcctgca caccgtcggc gatcgcctcg
      601 accccggtca gggcggtcgc gccggatgcg aacgctcgga gcaacaggaa cagcgtcacg
      661 gccgtgatcg gttcgatggg gatggaagac gtgtccgcga cgtggcaacc ggacagacat
      721 tgccagaagc ccaggacgat cgtgagtcca acgaccgcga cgaacccgta ggttggcagc
      781 gcgaagaacg ctcccgcctc cttcacaccg cgcaggttcg ctgccgtcac gaacgcgacg
      841 aggagtgacg cgagcaacac cttgtgctcc accatcgacg gggcggccga tgtgatcgcg
      901 acggtgccag ccgtcaccga cactgcgacc gtcaggatgt agtcggtgag gatcgcggcc
      961 gccgcgacga ggccggggac cgttccgagg ttctcgcgcg cgacgatgta cgaaccaccg
     1021 cctcgagggt acgcacgaac ggtctgccga taggaggtca ccacgatcgc caggagcgcc
     1081 gcgatcgcga gcgcgatcgg gagccggtac gagagggcgg cgacgccggc cgtcaccagg
     1141 acgagcatca tctcctcggt cgcataggcc accgaagaga gagggtccga cgagaagacc
     1201 ggcagcgcga gcgtcttggg gaggagttgg tgctcctgtg cggacgtcga gagcgcccgt
     1261 cccacgagga ggcgcttcag ggcgccgccg acccccgagc catcggtgcg cgtggccatc
     1321 ggaggatgct acgactcggc gtccgtgctt tccgcgatcc agcggtagcc gacgcccggc
     1381 tccgtcacga tcagctgtgg cgcctgcgca tcgtcgccga gcttcttgcg gagggcgcgc
     1441 acgtacgtcc ggaggtacgt cgtctccgtc ccgtacccct gcccccacac cttccgaagg
     1501 agccactgat gcgtgaggag ctttccggga tgcgtcgcga acgcctcgag cagctggtac
     1561 tcggtcggcg tcaggcgcag cgtctcgccg tccaggatca cgaggcgccg gcccaggtcc
     1621 atctcgagcg tgccgaaccg catcgtcggc gccggcggct cacccggttg cgcccgccgc
     1681 atcgccgccc gcgcccgcgc gagcagctcc tccatcgcga acggcttcgt cacgtagtcg
     1741 tcggcccccg cgtcgagcgc cgcgaccttg tcctgctgcg cgtcgcggac cgatagcacg
     1801 atgatcggga gctccgtgaa cccgcggacg cgacggatca cctcggtccc gtcgacgtcc
     1861 gggaggccga ggtccagcag catcagctcg ggggcgctct cggccgcgcc gatcaccgcc
     1921 tcctcgccgg tgcccacggt ctgcacctcg tacccgtgcg cttcgaggct ggtccggagg
     1981 gcacgtcgga tctgtggctc gtcgtcgacg accaacaccc ggttcacggg gccacctcct
     2041 cgcgcgggat ctcgaacgcc accaggcagc caccgtccgg gccgctctcg acccagatcc
     2101 gaccaccgtg cgccaccacg accgccttcg agatcgcgag ccccaaaccg ctgcccggtg
     2161 tctccgggct tgacgatcca cgatagaacg cctcgaagac ccgttcgcgt tcgtccgatg
     2221 gcacccctgg tccctgatcc gcgatccgga cctggacggc atctcggatc cactggacgc
     2281 cgacccggat ctcgctcccc tccggggcgt gccggccggc gttctcaagg aggttcgtga
     2341 gcacctggtc gagctggacc gggtcgacgg agacgtcggg gagatcggac gggaggtcgg
     2401 cggcgacggt gtgacgggcg agccggggtc gcatccgcgc caggacgacc tcgacgatct
     2461 catccatcgc cgcctgctgg cgtgtcggcg tgagggcgcc ggcccggatc cgcgcgaggt
     2521 cgagcaggtt gccgacgagc cgattcagcc ggtcggtctc ctcgaggatc gtcgtcagca
     2581 gctcccgctc ctgttcggga tcgtggtgca ccgcggcgtc caacaggctg gtgacgcccg
     2641 ccttgatcga ggcgagcggc gtgcggagat cgtgggtgac cgaggagaac atcgcggcgc
     2701 ggagctggtt cgtctcggcg tcgagctgtg cgatccgcgc gcgagcgtcc agccgcgcgc
     2761 gatcgagtgc cgcggtcgcc tggcgggcag ccgcctccag caggagctgg tcgtcgcgcg
     2821 agaacggccg gcctccctcg gggcgctccg cgacgagcct gccgatcgag gcctcccctg
     2881 ccagcagcgg gacggtcgtg cgcggccccg aggacgtctc gccgtcggtc ctggagacgg
     2941 cgcggagctc cgtcccgtcg agggtcgcct ccacctcgac cttcgacagg gcgaacggct
     3001 cgaggaggac ctcggcgaac tcgtggagga ctcggtccgg tacctcgcgg gtatgcagcg
     3061 tgctcgagag tgttcccagg aaccgcgcct ctcgctcgcg gcgggaggcg cgcgcccggt
     3121 cctcgctcgc ctgaccgacc aacagcccga cgatgagcgc caccgagagg aacacgacac
     3181 ccgtcgcgac gtcttccacg tcgtcgaagc cgaacgtgag ctgcgggtcc tcgacgacga
     3241 ggggccggat cgtcgaggcg aggatcccgg cgatgaggcc ggcccccaac ccgcccgcaa
     3301 cggcggcgat cacgaccgcc agcatgaaca gcgcgatcga gatctcgggt cgggcgggag
     3361 ccaacgggaa cgcgatcgcg gcggcgatgg cgggggtgat cagcccgatc accgttcccc
     3421 gcagggtgag cttcgcggtt ccctccgcct cgtgctcctc gctcacgacc acgacacgct
     3481 agcccctttc acgggatctt cacgccccga gaaggatcct tcacgcttcc ctcacggcct
     3541 tgtctcgaag atgagcgcag aggaacccat cctccgagtc aattccaggg gcccgacagc
     3601 tccctccagt tgttggacct ccggatccgt cctcatcatt cgggcccgct ctgcgggccc
     3661 gaactctttc tcgggtcgtc cacgtcctct cctccgggag ggacgagcac gcggaggatc
     3721 ctcttggtgc gcgctacacc ccggggtagg cttccgcacc gtatgcacgt gatcatcatg
     3781 ggttgtgggc gcgtcggatc cgagttggcc aacgcgatcg ccgacgagca cgacgtcgcg
     3841 atcatcgaca agaaccccga ggcgttccac gcctatcccc cc
//
LOCUS       DASUVE010000050         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1533529, whole genome shotgun sequence.
ACCESSION   DASUVE010000050 DASUVE010000000
VERSION     DASUVE010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533529"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VF036_01035"
     CDS             <1..672
                     /locus_tag="VF036_01035"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018639304.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX6263844.1"
                     /translation="EVGALLWVDASHFIGLVAGGAYPSPVPHADVVTFTTHKALRGPR
                     GAMIVCTEEHAKAIDKAVFPMMQGGPLEHVIAAKAVALKEAMQPAFREYAHRVVRDAR
                     ALAAGLADEGLRVISGGTDSHLILADVRPVGTDGKTAEAVCDEVGIALNKNQIPYDPN
                     PPSSPSGIRVGTPGPATLGMDEAEMGEVASIIGDVLKHPDDGSVKGKSGQRVRDLMER
                     FPLYP"
     gene            761..1984
                     /locus_tag="VF036_01040"
     CDS             761..1984
                     /locus_tag="VF036_01040"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX6263845.1"
                     /translation="MVAYLIVFLVAAGTTFALTPLVRRLAIRFGAIDEPSDRKLHTVP
                     TPTMGGLAMWAGFLVAIGVSRLLPFFEPMNATAVEPLAAVVTCTMMVGLGVIDDRRGT
                     SALAKLTAQIFIAGILILLGVQLAYFVLPGEGLAVLGTDEAVPLTILWVLAAANAINL
                     VDGLDGLAAGMVAIAAATFFIYMVRGADLSESASVAALLSAITVGVCLGFLPWNFHPA
                     KIFMGDTGSMLLGMLVAVATISGVGLNPFPPERGDVAVIALPLLVPLLVLAIPFLDVV
                     LAIVRRTRKGVGIMHADKEHIYHRLMDIGHGHRQTVLLMYLWSTLVCGSALAIGVIDG
                     LLVAGVIVAGAVTLFLVTALPRLLERRRNGDANGEGEEPRSAGPAGTAGTAVPSSGVT
                     TKPAPGAAPADPSAP"
BASE COUNT          313 a          759 c          773 g          365 t
ORIGIN      
        1 gaggtgggtg cgctgctgtg ggtcgatgcc tcccatttca tcggcctggt cgccggcggc
       61 gcctacccct cgcccgtgcc gcacgccgac gtcgtgacgt tcaccacgca caaggcgctc
      121 cggggacccc gcggggcgat gatcgtctgc acggaggagc acgcgaaggc gatcgacaag
      181 gccgtgttcc cgatgatgca gggaggcccg ctcgagcacg tgatcgccgc gaaagcggtc
      241 gcactcaagg aagcgatgca gccggcgttc cgcgagtacg cgcatcgcgt ggttcgggat
      301 gcgcgggcac tcgccgccgg cctggccgac gaaggtctcc gggtgatctc gggagggacc
      361 gactcgcatc tgatcctcgc ggacgtccgc ccggtcggca ccgacggcaa gacggccgag
      421 gccgtctgcg acgaggtcgg gatcgcgctg aacaagaacc agatcccgta cgaccccaac
      481 ccgccctcgt ccccctccgg gatccgcgtc ggcacaccgg gtccggcgac gctcgggatg
      541 gacgaggcag agatgggaga ggtggcgtcg atcatcggcg acgtcctgaa gcatccggac
      601 gacgggtcgg tgaaggggaa gtccggacag cgcgtccggg acctgatgga gcggttcccg
      661 ctctacccct gacgacgtcc gtggcgacgc cctgacgatc tcgtcgtgag cgggtccgcc
      721 ggttgccggg tggcgaggac ccccgcacac tgagaccacc gtggtcgcgt atctgatcgt
      781 gttccttgtc gcagcgggga ccaccttcgc gctgacgccg ctggtgcgac gcctcgccat
      841 ccggttcggc gcgatcgacg agccgtcgga ccgcaagctc cacaccgtcc ctacgccgac
      901 gatgggtggc ctcgcgatgt gggccggctt cctcgtggcg atcggggtct cccggctgct
      961 gccgttcttc gagccgatga acgcgaccgc cgtcgagccc ctcgcggcgg tcgtcacgtg
     1021 cacgatgatg gtcggcctcg gcgtgatcga cgaccggcgg ggcacctccg cgctcgcgaa
     1081 gctcacggcc cagatcttca tcgccggcat cctcatcctg ctcggcgtcc agctcgcgta
     1141 cttcgtcctc ccgggcgagg ggctcgcggt gctcgggacc gacgaggccg tgccgctcac
     1201 gatcctctgg gtgctggcgg ctgcgaacgc gatcaacctc gtggacgggt tggatgggct
     1261 cgcggccggc atggtggcga tcgccgccgc cacgttcttc atctacatgg tccgcggagc
     1321 cgatctctcc gagtcggcgt ccgtcgccgc gctcctgtcc gcgatcacgg tcggcgtctg
     1381 cctgggcttc ctcccctgga acttccaccc cgcgaagatc ttcatgggcg acacggggtc
     1441 gatgctcctc gggatgctcg tggcggtcgc cacgatctcg ggggtcggcc tgaatccctt
     1501 cccgccggaa cggggagacg tcgcggtgat cgcattgccg ctgctcgtgc ctctgctggt
     1561 cctcgcgatc ccgttcctcg acgtggtcct cgcgatcgta cggcggacgc ggaagggcgt
     1621 gggcatcatg cacgcggaca aggagcacat ctaccaccgg ctcatggaca tcggccacgg
     1681 tcaccggcag accgtgctgc tgatgtacct ctggtcaacg ctggtctgtg gctccgcgct
     1741 cgcgatcggg gtgatcgacg gcttgctcgt cgccggcgtg atcgtcgcgg gtgcggtcac
     1801 gctcttcctc gtgacggcgc tgccccgtct cctggagcgt cggcgcaacg gggatgcgaa
     1861 cggggagggc gaggagccgc ggtcggcggg tcccgccgga accgccggaa cggctgtgcc
     1921 ctcgtcgggc gtgaccacga agcccgcgcc gggcgcggct cccgcggatc cgagcgcccc
     1981 atgactggcg cgaccgtcgg atcgacgtcc ggtgcatgtg aaatcattca caagctcgcc
     2041 gacacaggtt gtggaaacag cctctgacca gcggaaacgc aggattccgc ggtttgacgg
     2101 cacgcgaagg ggccgattag agtgtccgcc tccgatgagc ggatcccgca agagcgatgc
     2161 gtggtccggc atggggatcg gctgggcgat cacctccacc ctcatcgggg
//
LOCUS       DASUVE010000051         4550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_26666, whole genome shotgun sequence.
ACCESSION   DASUVE010000051 DASUVE010000000
VERSION     DASUVE010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26666"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="VF036_01045"
     CDS             complement(<1..158)
                     /locus_tag="VF036_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014044958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease"
                     /protein_id="HEX6263846.1"
                     /translation="MHRLLLDVSSLTYRAFFAMPDSVTAPDGTPVNAVHGYLDMVTRL
                     VVARRPDEV"
     gene            complement(179..679)
                     /locus_tag="VF036_01050"
     CDS             complement(179..679)
                     /locus_tag="VF036_01050"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6263847.1"
                     /translation="MDADSLLAFVRFHAWANDRILTTAAVLADEELRRPGVLDHGSAF
                     ETLRHLVDVDRSWREACIGNDVGQTYVWDHGFVLDDLAAIHAFCLEEDVRLRSYVESL
                     DEAGLNEPLSDGDPREPRWLVVAHVVNHGTQHRSELAHYFTESGSSPGDLDLLAAYQL
                     PWPEPS"
     gene            775..1230
                     /locus_tag="VF036_01055"
     CDS             775..1230
                     /locus_tag="VF036_01055"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX6263848.1"
                     /translation="MDWYLPLKLVHVLSAIVAVGTNVTYFVWLASMKGRAPTQQTFAL
                     ETVEKLDARLANPAYGVLPLTGVIMVLISDGLGFTTFWIAVAIGLYIAVGVIAGGFFA
                     PSLRRQTDLAGEAGSPAYEAAAQRTRTTGLLTMLPIAGILYLMVMKPTP"
     gene            complement(1235..1507)
                     /locus_tag="VF036_01060"
     CDS             complement(1235..1507)
                     /locus_tag="VF036_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263849.1"
                     /translation="MARYREGTGTALLVAIGSFSFFAVLAWIVAALLWVALSIYALVE
                     MIGDGRPSAVAVLLIVVLLVGTLVALAAVGLGFVGKRLTPGKRDRR"
     gene            1552..3069
                     /gene="mptB"
                     /locus_tag="VF036_01065"
     CDS             1552..3069
                     /gene="mptB"
                     /locus_tag="VF036_01065"
                     /inference="COORDINATES: protein motif:HMM:NF038066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HEX6263850.1"
                     /translation="METTRSRTAWPAVAAAVAGFLLEAVVAAVPGSPLQPILAPGADP
                     GGPFVWAAETIQLDRLGDALPVASVIGVVAGMAGFLALLRAAWRGDLSLRFALGLSIA
                     FHLAIVLQPLLISRDVYSYIAHGQIVSVHHANPYVQTPADFPGFLSRTLVGPRWVDTP
                     SVYGPLFTHLAGLVTRTSPRLDVLVDAFRLIAIAASLATTFVIAAVSRGLRPERAAFA
                     VAAFGLNPVILFQSAASGHNDLLVALGIALAAWFVLRGWTYWATVLLACAGLIKATAF
                     LPLLLLVIWVAWRRSPGERLRAGLIHAAIAFAVVVVVAVPFLQWHDPTLGMLELSSHE
                     GWLAPSRFFRRVLDAISGDALGVVARVVFAVALFAAVVGLIRGVARAAASGRPVLELF
                     GGWGWALLVLTLLGPVLLPWYLAWSLPLIWILPRVPCASLIALSVVLALSQWTAEPLR
                     FPEAYDLNVLIGHYALTPIVIGLLVWLGLDLRRRLSIPLALHDEERREPAEARQH"
     gene            complement(2992..>4550)
                     /locus_tag="VF036_01070"
     CDS             complement(2992..>4550)
                     /locus_tag="VF036_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263851.1"
                     /translation="VGAASAALVRRATRGAEKTWLLAGLLTGVFSVHLVAGYLANLVV
                     GVAFLAAAACLAGPRRSWWAAAVLLAGGGLAHAPFLVHAIVVFAGTATLAWRAGARDE
                     ARDIALSVTAGGVVAGAGILATIAGPGPIAAETSRDGYLRRAGLDRQLVDAYRERFRL
                     RAARYVQWISVPLAVAGTAVADASAAGGFLRRFLASWLTVIAVGVPVGYITGWLPPDR
                     LVTFGFAVPIAAAIGLGWFRGRLGTRPWLAGMAVLALVAWMIFGALLAWGRQSSFISV
                     PEGRQATRAAAVSHLVAPGTPIVLPVDDGDPTSTFLSARAANVVRAALPSDRVGDVYV
                     FVGTVTDLFAGRPTARGDAEYDAISELTLAAIPDGPEPVVIVLPAFYRMPDAASDGRL
                     FEFGGGVLASLDSPFSPVPIESDDDRLRFGPSSGGGIVLATIVIAVVLTFLGLGYARS
                     VFADAVTAVATAPAFGAAAVTLAAVALDRIGLRLESTPVALAASVLAGLGGLAAFLVV
                     QRQRDRQAPA"
BASE COUNT          674 a         1605 c         1599 g          672 t
ORIGIN      
        1 acctcatccg gccggcgggc gacaacgagg cgcgtgacca tgtcgaggta cccgtggacc
       61 gcgttcaccg gtgtcccgtc gggcgcggtc accgagtccg gcatcgcgaa gaacgcccga
      121 taggtcaggc tcgatacgtc caggaggagt cgatgcacgc ggcagagcct agacgggctc
      181 aggaaggctc gggccacggg agctggtacg cggccaggag gtcgaggtcg cccggggagc
      241 tccccgactc ggtgaagtag tgggcgagct cgctgcggtg ctgcgtcccg tggttcacga
      301 cgtgggcgac gaccagccac cgaggctctc gggggtcgcc gtccgagagg ggctcgttca
      361 gacccgcctc gtcgagggac tccacgtagc tccggaggcg gacgtcctcc tcgaggcaga
      421 aggcatggat cgccgccagg tcgtcgagca cgaacccgtg gtcccagacg taggtctgcc
      481 cgacgtcgtt cccgatgcag gcctcacgcc agctccgatc gacgtcgacg agatgccgga
      541 gggtctcgaa ggcgctgccg tgatcgagca cccccggtcg ccggagctct tcatccgcga
      601 ggacggccgc cgtcgtcagg atgcggtcgt tcgcccaggc gtggaagcga acgaaggcga
      661 gcaggctgtc ggcgtccatc ggctcatcct agggctgcca tctgagcccg gaagcgggcg
      721 tgccgcctcg tagggcagta tgggttgcga tcggtccgcg gagaggggaa cgacatggac
      781 tggtacttgc cgctgaagct cgtgcacgtg ctgtcggcga tcgtcgcggt cgggacgaac
      841 gtgacgtact tcgtgtggct cgcctcgatg aaggggcgcg ccccgacgca acagacgttc
      901 gcactggaaa cggtcgagaa gctcgatgcg cgcctcgcga accccgcgta cggcgtgctg
      961 ccgctcaccg gtgtgatcat ggtcctgatc tccgacggcc tggggttcac gacgttctgg
     1021 atcgcggtcg cgatcgggtt gtatatcgcg gtcggggtca tcgccggtgg cttcttcgcg
     1081 ccgtcgctgc gtcgtcagac ggatctcgcg ggcgaggcag gctctcccgc gtacgaggcc
     1141 gcggctcaac gcacccggac cacgggactc ctcacgatgc tgccgatcgc cggcatcctc
     1201 tacctgatgg tgatgaagcc cacgccgtag acgatcagcg gcggtcgcgc ttccccggtg
     1261 tgagccgctt gcccacgaac ccgagcccga cggcggccag cgcgaccagg gttcccacga
     1321 gcaggacgac gatcagcagc accgcgaccg cgctgggacg gccgtcaccg atcatctcca
     1381 ccagcgcgta gatcgacagc gcgacccaga ggagcgccgc gacgatccac gcgaggaccg
     1441 cgaagaagct gaacgagccg atcgctacga gcagcgccgt gcccgtgcct tcccggtagc
     1501 gcgccatggg ctgcagccta gcggcgaccg ggtgctagcg tcggccgcga catggagacg
     1561 actcgctcgc ggacggcgtg gcccgccgtc gccgcggccg tggccgggtt cctgctggag
     1621 gccgtcgtcg ccgcggtccc cgggtctcct ctccagccga tcctggcccc gggcgccgac
     1681 ccggggggcc cgttcgtctg ggccgccgag acgatccagc tcgatcggct cggcgacgcc
     1741 ctccccgtgg cctccgtgat cggtgtggtg gcggggatgg cggggttcct cgcgctccta
     1801 cgagcggcgt ggcggggcga tctgtcgctc cgcttcgccc tcgggctttc gatcgccttc
     1861 cacctcgcga tcgtcttgca gccgctgctg atctcccggg acgtctattc gtacatcgcg
     1921 cacggccaga tcgtgtcggt gcaccacgcg aacccgtacg tccagacgcc cgccgacttc
     1981 cccggcttcc tgtcgcggac gctcgtcgga ccgcggtggg tcgacactcc atccgtctac
     2041 ggaccgctgt tcacccatct ggcagggctg gtcacccgga cgagcccgcg cctggacgtg
     2101 ctcgtcgacg cgttccgact catcgcgatc gccgcgagcc tcgccacgac gttcgtgatc
     2161 gccgcggtgt cccgcgggct ccgcccggaa cgagcggcgt tcgcggtggc cgcgttcggc
     2221 ctgaaccccg tgatcctctt ccagtccgcc gcgagcgggc acaacgatct cctggtggcg
     2281 ctcgggatcg cactcgccgc ctggttcgtc ctccgagggt ggacgtactg ggcgaccgtg
     2341 ctcttggcgt gcgcaggact gatcaaggcc acggcgttcc tcccgctgct cctgctcgtg
     2401 atctgggtgg cgtggcgacg gtcgcccggg gaacgcttgc gcgccgggct gatccacgcc
     2461 gccatcgctt tcgccgtggt cgtcgtcgtc gcggttccct tcctccaatg gcacgacccc
     2521 acgctcggga tgctcgagct gtcatcgcac gagggatggc tcgcgccgtc ccgcttcttc
     2581 cgacgtgtcc tcgacgcgat cagcggggac gcgctgggcg tcgtcgcgcg cgtcgtgttc
     2641 gcggtggccc tcttcgccgc ggtcgtgggg ttgattcgcg gggtcgcgcg cgcggccgcg
     2701 tcggggcgcc cggtgctcga gctcttcggg ggatgggggt gggcgctgct cgtcctgacc
     2761 ctgctcggac cggtgctcct gccgtggtac ctcgcgtggt ccctcccgct gatctggatc
     2821 ctccctcggg tgccctgcgc ctcgctgatc gcgctctcgg tcgtgctcgc gctgtcgcag
     2881 tggacggcgg agccgctccg gttccccgag gcgtacgacc tgaacgtcct gatcggccac
     2941 tacgcgctga ccccgatcgt gatcggtctc ctcgtgtggc tgggcctcga cctacgccgg
     3001 cgcctgtcga tcccgctggc gctgcacgac gaggaacgcc gcgagcccgc cgaggcccgc
     3061 cagcactgag gcagcgagcg cgaccggcgt cgactccagg cggagcccta tccgatcgag
     3121 cgcgaccgct gcgagggtga ccgcggcggc gccgaaggca ggtgccgtcg cgaccgcggt
     3181 cacggcatcg gcgaacaccg acctcgcgta tccgagtccg aggaaggtga gtacgaccgc
     3241 gatcacgatc gtggcaagga cgatcccccc gcccgaggaa ggtccgaagc gaagacgatc
     3301 gtcgtcgctc tcgatgggca ccggggagaa cggactgtcc agcgaggcaa ggacgccgcc
     3361 cccaaactcg aagagccgcc cgtccgaagc cgcatccggc atccgataga aggcgggaag
     3421 cacgatcacc accggctcag gaccgtccgg gatcgcggcg agcgtgagct cggagatggc
     3481 gtcgtattcc gcatcacctc gtgcggtcgg tcgtcccgcg aacaggtcgg tgacggttcc
     3541 gacgaagaca tacacatcac cgacacgatc cgacggaagg gctgctcgga ccacgttcgc
     3601 tgcccgagcc gataggaagg tcgatgtggg gtcgccgtcg tcgaccggaa gcacgatcgg
     3661 agtcccggga gcaacgaggt gagagaccgc cgccgcccgg gtcgcttgtc gtccctcggg
     3721 gaccgagatg aaactcgact gccgacccca tgcgagcagc gcaccaaaga tcatccatgc
     3781 gacgagggcg aggacggcca tacccgccag ccatggacgc gttcccagcc ggccacggaa
     3841 ccatccgagc ccgatggccg cagcgatcgg cacggcgaac ccgaacgtga cgagccggtc
     3901 cggggggagc catccggtga tgtagcccac agggacaccg accgcgatca ccgtgagcca
     3961 cgacgcgagg aaccgccgca ggaaccctcc cgccgccgag gcatcggcga ccgcggtccc
     4021 agcgacggcg agcgggaccg agatccactg aacgtagcgg gccgcgcgaa gccggaaccg
     4081 ctcgcggtac gcgtcgacga gctgtcgatc gagtcccgcc cgtcggaggt atccgtccct
     4141 cgacgtctcg gcggcgatcg gaccgggtcc cgcgatcgtc gcgaggatgc cggctcccgc
     4201 gacgacgccg cctgcggtga cggacagcgc gatgtcccgg gcttcgtcgc gggcacccgc
     4261 tcgccaggcg agcgtcgcgg tacccgcgaa cacgacgatc gcgtggacca ggaagggggc
     4321 gtgtgccagg ccaccgcccg cgaggagcac ggccgctgcc caccacgacc ggcggggtcc
     4381 tgcgaggcac gccgcggccg cgaggaacgc gacgccgacc acgagattgg cgaggtagcc
     4441 ggcgacgaga tggacggaga agacgcccgt gaggagcccc gcgaggagcc acgtcttctc
     4501 cgcgccgcgg gtggcacggc ggaccagcgc cgccgacgcc gctcccacgg
//
LOCUS       DASUVE010000052         4671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_968605, whole genome shotgun sequence.
ACCESSION   DASUVE010000052 DASUVE010000000
VERSION     DASUVE010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968605"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VF036_01075"
     CDS             complement(<1..203)
                     /locus_tag="VF036_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263852.1"
                     /translation="MVESTVRTPGRKEDADPMKAQRRAGNFGTLALLSVLALVAVACG
                     DGNGGGGGGGGGDTGEDGVIRFT"
     gene            complement(328..1041)
                     /locus_tag="VF036_01080"
     CDS             complement(328..1041)
                     /locus_tag="VF036_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6263853.1"
                     /translation="MADGLRLETAGTTLVATIDAGESNLFTREMMDALVDGIVDAGRA
                     EEMRFVRLRAAGESFCMGRAKGGSTPDELRGMARRIGTVNETIRTSPLTVLTEVNGPA
                     AGFGVGMIGGSDIAVGADTATFAFPEIKAGFAPAVVISWAQYQLPRRLLYDMVSTGDA
                     IDAATAVGGGLLTEAVPADELEARVDERIARLEEVDAFALREMKRFLVFTRAMDPATA
                     AQASVDTLVFSGLRVIGKF"
     gene            complement(1034..2392)
                     /locus_tag="VF036_01085"
     CDS             complement(1034..2392)
                     /locus_tag="VF036_01085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6263854.1"
                     /translation="MAQLAAAGLSGTVVNVSVTVAVVAIAAEFRASIAAAAATVVLLN
                     VAMAFTMPLAGAWSARLGPRRLLAWAGLIVLGSSVLLSLAPNLFVLAVARAAQGAGLA
                     AVVPVSVQASGQLLVGEQRARALGWWGASNGLGLAFAPLIGGAIVDLVGWRWVTVPSC
                     LLGVALVVTALQAIPTDLPHGSAVRFRGLGLIALVTGTAMSALAAASAEAWLIAGALA
                     LVCAVAGVIAGRATRPGGELADLRRWAHDRGVRRSSLGATLQMVANGLVQVGVPAWLI
                     VNGLLGAGQAAAILMGMTLTMAAMGPITGKRAKVRYPSRLRRGLAGCAAGLLGLAVAA
                     ILGPWWLAAPSLVVLGLGAGSLLSPSLTAFSQTEAGASTVALSLFNLARLGAFGIGGL
                     LGGASVDAGVPGSAFLLVAVLCGVAATRVGSIEEREATSAVDARDGRRAPDGAGGGTD
                     HG"
     gene            complement(2470..2808)
                     /locus_tag="VF036_01090"
     CDS             complement(2470..2808)
                     /locus_tag="VF036_01090"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6263855.1"
                     /translation="MPFLVFDEMIKETVTPKYSTAHGELVTGEFIEVGRLSFQKGTGA
                     VEHAHPHEQVMYIISGKLAVDLEGEDHAELGPGMGFHAKPNQKHRVQAVEDTQVISSK
                     HVIEGVGHKI"
     gene            complement(2860..3207)
                     /locus_tag="VF036_01095"
     CDS             complement(2860..3207)
                     /locus_tag="VF036_01095"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6263856.1"
                     /translation="MGKISFDRDDDNLVTPQYSEARGPVLRSERIEVTKAMFAKDKGA
                     KTHAHPEEQVFYIASGRLRVTCDGETYEVGPGEGSYHPSNAEHTVTALEDTVAISFKN
                     LVDSIYEPTGELK"
     gene            complement(3253..3993)
                     /locus_tag="VF036_01100"
     CDS             complement(3253..3993)
                     /locus_tag="VF036_01100"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6263857.1"
                     /translation="MATVTVADLRKIFPGEGRDVVALEDVSFEASGHTFVSIVGPSGC
                     GKSTLLNILAGVESPTAGRAEITEDGSRARVGYVFQAPRLLPWRSVMDNMLFVQPERT
                     DETVARAARYLDMVGLADYHKAWPAQLSGGMQQRVGIARAFSIEPDALLMDEPFSHLD
                     AITARGLRRELHSMWMETKKTVLFVTHDVGEAVELSDRIVILAKGGKLRQDLTVDLPY
                     PRDPADDKVALMKAEVLREFEELDLIAT"
     gene            complement(4020..>4671)
                     /locus_tag="VF036_01105"
     CDS             complement(4020..>4671)
                     /locus_tag="VF036_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6263858.1"
                     /translation="LDGRLLEAFSVSLLRLAIGFALALLVGSIVGLAMGLSSRVEAML
                     HDFVIVGLTFPYLIWGLLVAMWWGYAWYGPVLVVIIAGLPYVILNMSEGVRDVSKELR
                     DMANAYEVPRDRVMRHLILPSLSPFFFASLRYGLANGWKGLILAEVFAATSGAGWHIT
                     SMRDYGDFAGVVGFAIYFALFSIVVERLVFGRLSRRVFRWRPTLAREMSEPPELTV"
BASE COUNT          753 a         1697 c         1537 g          684 t
ORIGIN      
        1 aacgtgaacc tgatgacgcc gtcctcgccg gtgtcgccgc ctcctccgcc gcccccgccg
       61 ccgttcccgt ctccacacgc caccgccacg agagcgagca cgctcaggag cgcgagcgtc
      121 ccgaagttcc cagccctgcg ctgcgccttc atcggatccg cgtcctcctt ccgtccgggg
      181 gtccggaccg tcgattcgac catggatcct cggcgttccc cgggagggga gcgcactcca
      241 tgactatata taatgtgcag cttggacgtt tcgaccttgg cgtgtccggc tcgcgccgtc
      301 aaggcaacga acttcgagac gaagggttca aaacttcccg atcacgcgga gcccgctgaa
      361 cacgagggtg tccaccgacg cctgcgccgc cgtcgcgggg tccatcgccc tcgtgaagac
      421 caggaaccgc ttcatctccc ggagcgcgaa cgcatcgacc tcttcgaggc gggcgatgcg
      481 ctcgtccacg cgggcctcga gctcgtctgc cgggacggct tccgtcagca gccctccgcc
      541 gacggcggtg gccgcatcga tcgcgtcgcc cgtcgagacc atgtcgtaaa gcagccggcg
      601 ggggagctgg tactgcgccc agctgatcac cacggccggt gcgaagccgg ccttgatctc
      661 cgggaaggcg aacgtcgcgg tgtccgcgcc gacggcgatg tcgctccctc cgatcatccc
      721 cacgccgaag cccgccgccg gcccgttcac ctcggtgagg acggtgagcg ggctggtgcg
      781 gatcgtctcg ttcaccgtcc cgatccgccg ggccatcccg cgcagctcgt cgggcgtgga
      841 cccgcccttc gcgcgcccca tgcagaatga ctcgcccgcc gcccggagcc ggacgaagcg
      901 catctcctcg gcacgtcccg cgtcgacgat cccgtcgacg agcgcatcca tcatctccct
      961 ggtgaacagg ttgctctcgc ccgcatcgat cgtggccacc agcgtggtgc ccgcggtctc
     1021 gagccggagt ccgtcagcca tgatccgtac ctcctcctgc gccgtccggc gctcttcgtc
     1081 catctcgtgc atcgaccgcg ctcgtcgcct cacgctcctc gatggagccc acccgcgtcg
     1141 ccgcgacccc gcagagcacg gcgacgagga ggaacgccga cccgggaacc cccgcgtcga
     1201 cagacgcgcc gcccaggagt ccgccgatcc cgaacgcacc gagccgagcg aggttgaaca
     1261 gggacaacgc cacggtgctc gctcctgcct cggtctggct gaacgccgtc agggacggag
     1321 agagcaagga gcccgctccg aggccgagga ccacgagcga cggcgcggcg agccaccagg
     1381 gccccaggat ggccgccacc gcgagtccca agaggccggc tgcgcatccc gccagtcccc
     1441 gtcgcagtcg ggagggatag cgcaccttcg ctcgcttccc tgtgatcgga cccatggccg
     1501 ccatcgtcag ggtcatcccc atcaggatcg cggccgcctg cccggcgccg aggagtccat
     1561 tcacgatcag ccacgccggc acgcccacct ggacgaggcc gttcgcgacc atctgcagcg
     1621 tggcaccgag cgacgaccgc cggacgccgc ggtcgtgggc ccatcgacgg aggtccgcga
     1681 gctccccgcc cgggcgtgtc gcccgcccgg cgatcacccc ggccacggcg catacgaggg
     1741 cgagcgcgcc ggcgatcagc cacgcctcgg ccgacgccgc cgcgagcgcc gacatcgccg
     1801 tgccggtgac cagcgcgatg aggccgagtc ctctgaaccg cacggcggaa ccgtgcggca
     1861 ggtcggtggg gatcgcctgg agcgccgtca caacgagcgc gacccctagg aggcacgacg
     1921 ggaccgtcac ccaccgccag cccacgaggt cgacgatcgc tcctccgatc agcggggcaa
     1981 aggcgagccc caacccgttc gaggctcccc accagccgag cgcacgtgcc cgctgttcgc
     2041 ctaccaagag ctgacccgac gcctgaacgg agacgggaac gacggccgcg agcccggctc
     2101 cctgcgccgc gcgcgcgacg gccaggacga agaggttggg ggcgagggac aggaggaccg
     2161 aggagccgag cacgatgagc ccagcccagg cgaggagccg ccgagggccg agccgagcgg
     2221 accaggcgcc ggcgagcggc atcgtgaacg ccatcgccac gttcagcagc acgaccgtcg
     2281 cggccgccgc ggcgatcgag gcgcggaact cggcggcgat cgcgacgacc gccaccgtca
     2341 cagagacgtt cacgaccgtg cccgacagac cggcggccgc gagctgcgcc accacccacc
     2401 ccggagacgt cggctcccga agggcgggcc tcgcgggggt cggtggaggc gacgagggtg
     2461 tcggcgcgct cagatcttgt ggccgacgcc ttcgatcacg tgcttcgagc tgatcacctg
     2521 ggtgtcctcg accgcctgga cccggtgctt ctggttcggc ttcgcgtgga agcccatgcc
     2581 cggtcccagc tcggcgtggt cctcaccctc gaggtccacg gcgagcttgc cggagatgat
     2641 gtacatcacc tgctcgtgcg ggtgggcgtg ctcgaccgcg cccgttccct tctggaagct
     2701 caggcggccc acctcgatga actcgccggt aacgagctcc ccatgggcgg tggagtactt
     2761 cggcgtcacg gtctccttga tcatctcgtc gaacacgagg aacggcatgt cgggtgctcc
     2821 tttccgtctg ccgagggatc gtcgcgacct gggtccggcc tacttcagct cgccggtcgg
     2881 ctcgtagatc gagtcgacga ggttcttgaa gctgatcgcg acggtatcct cgagcgccgt
     2941 cacggtgtgc tccgcgttgg aaggatggta actgccctcg ccgggcccga cctcgtaggt
     3001 ctcgccgtcg cacgtgacgc gcagccgtcc gctcgcgatg tagaagacct gctcctcggg
     3061 gtgggcatgc gtcttcgcgc ccttgtcctt cgcgaacatc gccttcgtca cctcgatcct
     3121 ctcgctgcgc aggacggggc cgcgtgcctc ggagtactgc ggtgtgacga ggttgtcgtc
     3181 gtctcggtcg aacgagatct tgcccatcgt ggtcctcctt cgtcggtgtc gggactgggt
     3241 gggaacgatg atctaggtcg cgatcaggtc cagctcctcg aactcgcgca gcacctccgc
     3301 cttcattagg gcgaccttgt cgtccgccgg gtcgcgcggg taaggcaggt cgacggtcag
     3361 gtcctgacgc agcttgccgc ccttcgccag gatcacgatg cggtcggaga gctccaccgc
     3421 ctcgccgacg tcgtgggtga cgaacaggac ggtcttcttc gtctccatcc acatcgagtg
     3481 cagctcacgg cgcaggcccc gcgccgtgat cgcgtcaagg tggctgaagg gctcgtccat
     3541 caggagcgcg tcgggctcga tcgagaacgc ccgggcgatc ccgacgcgct gctgcatgcc
     3601 gcccgagagc tgcgccggcc acgccttgtg gtagtcggcc agcccgacca tgtccaggta
     3661 gcgagccgcg cgggccacgg tctcgtcggt gcgctccggc tgcacgaaca gcatgttgtc
     3721 catcaccgaa cgccacggga ggaggcgggg cgcttggaag acgtacccga cgcgggcacg
     3781 cgacccgtct tcggtgatct cggcccggcc cgcggtcggc gactcgacgc cggccaggat
     3841 gttcagcagc gtggacttcc cgcagcccga aggtccgacg atgctgacga acgtgtgacc
     3901 ggaggcctcg aacgacacgt cttcgagggc gacgacgtcg cgtccctcgc ccgggaagat
     3961 cttccggaga tcggccacgg tcacggtcgc cacgggtcaa gcctcctcgt ctggttcggt
     4021 catacggtca gttccggggg ttcgctcatc tcccgcgcca atgtcggtct ccagcggaag
     4081 acccgccggg acagtcgtcc gaacacgagc cgctcgacca cgatcgagaa cagcgcgaag
     4141 tagatcgcga atccgaccac gcctgcgaag tcgccgtagt cgcgcatcga ggtgatgtgc
     4201 cagccggcgc cggatgtcgc cgcgaacacc tcggcgagga tcagtccctt ccagccgttc
     4261 gcgagtccgt agcggagcga cgcgaagaag aacgggctga gcgacggcag gatcaggtgc
     4321 cgcatcaccc ggtcgcgagg gacctcgtac gcattcgcca tgtcccggag ctccttcgag
     4381 acgtcacgca cgccctcgga catgttcagg atcacgtacg gcaagccggc gatgatcacc
     4441 accagcacag ggccgtacca agcgtagccc caccacatcg cgaccaggag cccccagatc
     4501 agatagggga acgtgagccc gacgatcacg aagtcgtgca gcatcgcctc gacacgcgag
     4561 gagagtccca tcgcgaggcc gacgatcgag ccgacgagga gggcgagcgc gaagccgatc
     4621 gcgaggcgca ggagactcac cgagaacgcc tccagcagtc tgccgtccag c
//
LOCUS       DASUVE010000053         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1157419, whole genome shotgun sequence.
ACCESSION   DASUVE010000053 DASUVE010000000
VERSION     DASUVE010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157419"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VF036_01110"
     CDS             complement(<1..265)
                     /locus_tag="VF036_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6263859.1"
                     /translation="MTVMVTGASGLLGHAIVRALVERDEVRATVRRPAASEPLRALGA
                     KVAVVSVEEPDALIEILPRVHTIVHLVGGVNQPRDDEILAANHG"
     gene            341..1426
                     /locus_tag="VF036_01115"
     CDS             341..1426
                     /locus_tag="VF036_01115"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEX6263860.1"
                     /translation="MDLRREQRKPQRVTERNTLTPIALATAIALVTVACDAKDGVRPR
                     EAASARESTTIVPETPAGLSFGDPRTEAARHLFEVEAEHGEYWRMFTLDRYDGESWTS
                     TDPDGSVGGVLLTAPTTLPRSGSEPPPGAETLDQTFRILSDFGTAGALVMAQTAEEIA
                     GPIGDITWDPERSQAFIDAHLEAGTEYAVRSRIVIPTPGELDRAKQAAPPTNLQWTEL
                     PADLDPRIADIAERWTAEVTSDYRKVLAIQQHFHNGDFVYSTDVDGGVDADSLLEFLT
                     RSRIGFCQHYSSAMAILVRTLGVPARIGVGFRSGTRQEDGSYLVKTTDAHVWVEVQFA
                     GYGWLQFEPEPGPVPHPNAQPGTYLNP"
     gene            1423..>2008
                     /locus_tag="VF036_01120"
     CDS             1423..>2008
                     /locus_tag="VF036_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX6263861.1"
                     /translation="MIPGPADVERVRRALGATPVAWRAVPGHGAASTRRFVVELPSGA
                     TAFVKIAAFDYAAEWFRDERLAYDALDGMPCLPRLLGWDDDGEAPLLALEDLSAARWP
                     PPWDRSSVDAVLSALDALHAGTPRSKLPTADERQFGLDSWSDVTADPGPFLATEICSP
                     EWLTDYLPALAASSAAARIEGEHVLHFDVRSDNLC"
BASE COUNT          318 a          703 c          685 g          302 t
ORIGIN      
        1 agccgtggtt cgcggcgagg atctcgtcgt ctctcggctg gttcacgccg cccaccaggt
       61 gcacgatcgt gtggacacgc gggaggatct cgatcagcgc gtccggctcc tccaccgaga
      121 cgacggcgac cttcgcgccg agggctcgca gcggctcgct cgccgcggga cgccggacgg
      181 tcgcccggac ctcgtcgcgc tccacgagcg cgcggacgat agcgtgcccg aggaggccgg
      241 acgcgcccgt gaccatcacc gtcatcaccg ccgatgctat ccatgcccgc tgtcgacgtt
      301 gaccgtacga tggcccgact gagcccggat cgagcccgac gtggatcttc gacgtgagca
      361 gcggaaaccc cagcgtgtca ccgaacgcaa cacgctcacc ccgatcgccc tggcgacggc
      421 gatcgcactg gtcacggtgg cgtgcgacgc gaaggacggc gtcagaccgc gcgaggccgc
      481 atccgccagg gagtcaacca cgatcgttcc ggagactccc gcgggcctgt cgttcggcga
      541 tccgcgaacg gaggcggcgc gtcacctgtt cgaggtcgag gccgagcacg gcgagtactg
      601 gcgcatgttc acgctggacc ggtacgacgg tgagtcctgg acgagcaccg accccgacgg
      661 atccgtgggg ggtgtgctgt tgacggctcc cacgaccctg ccgcggtccg gaagcgagcc
      721 gccgcccggg gcagagaccc tggaccagac cttccggatc ctcagtgact tcggcacagc
      781 cggcgcgctc gtgatggcac aaacagctga ggagatcgcg ggaccgatcg gcgatatcac
      841 gtgggatccc gaacgcagcc aggcgttcat cgatgcccat ctcgaggccg ggacggagta
      901 cgccgtccgc tcgaggatcg tcatcccgac gccgggggag ctggatcggg cgaagcaggc
      961 ggcccctccc acgaacctcc agtggaccga gctccctgcc gacctcgatc cccggatcgc
     1021 agacatcgcc gagcgatgga ctgcagaggt cacatcggac tatcggaagg tgctggcgat
     1081 ccagcagcat ttccacaacg gagacttcgt ctacagcacc gatgtcgacg gcggcgtcga
     1141 cgccgattcg ctgctcgagt tcctcacgag gtcgaggatc gggttctgtc agcactattc
     1201 gtccgcgatg gcgatcctgg tgcggacgct cggggtgccg gcacgcatcg gggtcgggtt
     1261 ccggtcaggc acacggcagg aagacggcag ctacttggtc aagaccacgg acgcccacgt
     1321 ctgggtggag gtgcagttcg ctggctacgg atggctccaa ttcgaaccgg agccgggccc
     1381 ggtccctcat ccgaacgcgc agcccggcac ctacctgaac ccttgatacc tggccccgcc
     1441 gacgtggagc gcgtgcgccg tgccctcggg gcgacgccgg tcgcctggcg tgcggtaccc
     1501 gggcacggcg ccgcgtcgac ccgccggttc gtggtggagc ttcccagcgg cgcaacggcg
     1561 ttcgtcaaga tcgccgcatt cgactacgcg gcggagtggt tccgagacga acgcctggcg
     1621 tacgacgcgc tcgacggcat gccctgcctt cctcggctcc tcgggtggga cgacgacggc
     1681 gaggccccct tgctggcgct cgaagacctg tcggccgcac gatggcctcc cccgtgggac
     1741 cgctcgagcg tggacgccgt gctcagcgca ctcgacgcgc tccatgcggg gacgccgcgc
     1801 tcaaagctcc ccaccgcgga tgagcgccag ttcgggctcg attcgtggtc cgacgtgacg
     1861 gccgacccgg gaccgttcct cgcgaccgag atctgctcgc ccgagtggct gacggattac
     1921 ctgccggcgc tcgcggcctc gtcggcggcg gctcggatcg aaggcgagca cgtgctgcac
     1981 ttcgacgttc ggagtgacaa cctctgcc
//
LOCUS       DASUVE010000054         2658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_404405, whole genome shotgun sequence.
ACCESSION   DASUVE010000054 DASUVE010000000
VERSION     DASUVE010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404405"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VF036_01125"
     CDS             complement(<1..280)
                     /locus_tag="VF036_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX6263862.1"
                     /translation="MSGHSKWATIKRKKAANDAKRSNQFAKLLRAIEVAAREGGGDPA
                     ANMTLASAVEKARASSVPGDNIDRAIKRGSGSDADGARYEEITYEGYAP"
     gene            complement(287..847)
                     /gene="pdxT"
                     /locus_tag="VF036_01130"
     CDS             complement(287..847)
                     /gene="pdxT"
                     /locus_tag="VF036_01130"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013005003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX6263863.1"
                     /translation="MKVGVLALQGDFREHVRVLATAGAVPVEVRTATELADVDGLVIP
                     GGESTTIAKLAHAGDLVDPLRERARDGMPILGTCAGMIVMAERVLDGEPLLSLMDVTV
                     RRNAYGRQVDSFEADVDVDGIDHLVRGVFIRAPWIEGVGSEVRVLSSYEGRPVVLQEG
                     NLLAASFHPELVGETALHEYFLSLPR"
     gene            complement(858..1739)
                     /gene="pdxS"
                     /locus_tag="VF036_01135"
     CDS             complement(858..1739)
                     /gene="pdxS"
                     /locus_tag="VF036_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX6263864.1"
                     /translation="MQRETGTFTVKSGLAEMLKGGVIMDVTTAEQAKIAEDAGACAVM
                     ALERVPADIRRDGGVARMADATKVEEIQRAVSIPVMAKARIGHFVEAQILQSLGVDYI
                     DESEVLTPADEEYHIDKWAFTVPFVCGARDLGEALRRIAEGAAMIRTKGEAGTGNVVE
                     AVRHQRAMLRQIAEVTQLRPEQYVTKAKELGAPVDLVRMVAEQGKLPVVNFAAGGLAT
                     PADAALMMQLGVDGVFVGSGIYKSEDPAPRARAIVEATTHFNDPEILAKVSHGLGEPM
                     HGLEIGSLETRLQDRGW"
     gene            complement(1839..>2658)
                     /locus_tag="VF036_01140"
     CDS             complement(1839..>2658)
                     /locus_tag="VF036_01140"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEX6263865.1"
                     /translation="RDAYPLAGLERADVIYEEPVEGGITRFAALFQCRDAGRVGPVRS
                     ARTTDPKILLQLSDQPILAFSGAHPKVTAALEEADLLQLTETSGNVAYERDDARSAPH
                     NLFVATRPLFNLATKAGVDLSLPPALFRYGEDVPTPSKRRSSATVAFSSSNVVEWDWD
                     GTRWVRQLDGAPMLSEGGAEISADNVVIQEVVVTESDILDVTGSPSPEVELLGEGRAW
                     ILRDGRLVVGRWRRDSLGDVTVFETRAGEEIDLAPGTTFVELMPKDGNGATFGR"
BASE COUNT          375 a          974 c          869 g          440 t
ORIGIN      
        1 ccggtgcgta tccctcgtag gtgatctcct cgtaccgagc gccgtccgcg tcggaaccgc
       61 tcccccgctt gatcgcgcgg tcgatgttgt cgccggggac cgaggaggcc ctcgccttct
      121 cgaccgccga cgcgagcgtc atgttcgccg cggggtcgcc gccgccttcc cgcgccgcga
      181 cctcgatcgc gcggaggagc ttcgcgaact ggttcgagcg cttcgcgtcg ttcgctgcct
      241 tcttccgctt gatcgtcgcc catttcgaat gaccggacat tcgcacctac ctcgggaggg
      301 aaaggaagta ctcgtggagg gcggtctcgc cgaccagctc ggggtggaac gaggccgcga
      361 gcaggttgcc ctcctgcagg acgaccggcc ggccctcgta cgacgagagc acgcggacct
      421 cggagcccac gccctcgatc cacggggcgc ggatgaacac cccgcggacc aggtggtcga
      481 tcccgtcgac gtccacgtcg gcctcgaacg aatccacctg tcggccgtag gcgttgcgcc
      541 ggacggtgac gtccatcagc gaaagcagcg gctccccatc gagcacccgc tccgccatca
      601 cgatcatccc ggcacacgtg ccgaggatgg gcatcccgtc ccgcgcacgt tcccgcaggg
      661 gatccaccag atcgcccgca tgcgccagct tcgcgatcgt cgtcgactca ccgccaggta
      721 tcacgagccc gtcgacgtcc gcgagctccg ttgccgttcg gacctcgacc gggacggcgc
      781 cggcggtcgc aagcacgcgg acgtgctcac ggaaatcacc ttgcagggcc aggacgccga
      841 ccttcaagga ccgctcctta ccagccgcga tcttggagcc gggtctcgag cgagccgatc
      901 tcgagtccgt gcatcggctc cccgagtccg tgcgagacct tcgccaggat ctccgggtcg
      961 ttgaagtgcg tcgtcgcctc gacgatcgcc ctagcgcgcg gcgcggggtc ctcggacttg
     1021 tagatgcccg acccgacgaa gacgccgtcg accccgagct gcatcatcag cgctgcatcg
     1081 gccggcgtgg ccagcccgcc cgccgcgaag ttcacgactg ggagcttgcc ctgctccgcg
     1141 accatgcgca cgaggtccac gggcgcgccg agctccttcg ccttcgtgac gtactgctcc
     1201 ggccggagct gggtcacctc cgcgatctgc cgcagcatcg cccgctggtg acggaccgcc
     1261 tccacgacgt tgcccgtccc cgcctcgccc ttcgtgcgga tcatcgcggc gccttcggcg
     1321 atgcggcgga gggcctcgcc gaggtcgcgg gctccacaga cgaacggcac ggtgaacgcc
     1381 cacttgtcga tgtggtactc ctcgtcggcg ggcgtgagga cctcggactc gtcgatgtag
     1441 tcgaccccca gggattggag gatctgcgct tcgacgaagt gcccgatccg cgccttcgcc
     1501 atcaccggga tcgagacggc ccgctggatc tcctcgacct tcgtcgcgtc cgccatccgc
     1561 gcgacgcctc cgtcgcgtcg gatgtcggcc ggcacgcgct cgagcgccat caccgcgcat
     1621 gcgccggcgt cttccgcgat cttcgcctgc tcggctgtgg tgacgtccat gatgacgccg
     1681 cccttcagca tctcggccag gccggacttc accgtgaagg tgccggtttc gcgctgcatt
     1741 cgccctcctt cgctctgccc tggcctctgc cgtggcgcct gcgctggtgc gctgggatgg
     1801 cccgagtgta ccgccgtcta cctgcgagga agccggcgct agcgcccgaa cgtcgctccg
     1861 tttccgtcct tcggcatcag ctcgacgaac gtcgtgccgg gcgcgaggtc gatctcctcg
     1921 ccggcccggg tctcgaagac cgtcacgtct ccgagcgagt cgcgccgcca ccggccgacg
     1981 acgagccggc cgtcgcgcag gatccacgca cggccctctc cgagcagctc cacctccggc
     2041 gacggggacc cggtcacgtc gaggatgtcg gattccgtga cgacgacctc ctggatcacg
     2101 acgttgtcgg ccgagatctc cgcgccgccc tccgagagca tcggcgcgcc gtccagctgc
     2161 cggacccacc gagttccgtc ccagtcccac tcgaccacgt tcgaactgga gaacgcgacc
     2221 gtcgcggacg agcgacgctt gctgggagtg ggaacgtcct cgccgtagcg gaagagtgcc
     2281 ggcggaaggg agagatcgac ccctgctttc gtggcgaggt tgaacagcgg cctcgtcgcg
     2341 acgaagaggt tgtgcggggc ggatcgcgcg tcgtcgcgct cgtacgcgac gttcccggac
     2401 gtttcggtga gctggaggag atccgcttct tcgagggcgg cggtcacctt cggatgcgct
     2461 cctgagaacg cgaggatcgg ttgatccgag agctggagca ggatcttggg gtccgtcgtc
     2521 ctcgcgctcc gcaccggtcc tacgcgcccc gcgtcacggc actggaagag cgcggcgaac
     2581 cgggtgatgc cgccctccac gggctcctcg tagatcacgt cggcgcgttc gagacccgcg
     2641 agcggatacg cgtcgcgg
//
LOCUS       DASUVE010000055         4506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_217204, whole genome shotgun sequence.
ACCESSION   DASUVE010000055 DASUVE010000000
VERSION     DASUVE010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217204"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VF036_01145"
     CDS             complement(<1..300)
                     /locus_tag="VF036_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018658212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX6263866.1"
                     /translation="METQVGVGAEQATSEIRRGVGATIGVLAAAVALGTAELLSAVFG
                     PGSSPIVAVGGAAVDATPEWLKSFAIRTFGEHDKRVLLIGIGIVLFIGVAIEGAAS"
     gene            396..1961
                     /locus_tag="VF036_01150"
     CDS             396..1961
                     /locus_tag="VF036_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6263867.1"
                     /translation="MPRRLLALVSSLGLVLLLLAPTARAAPITVKVMTLNVFYGGDEW
                     NLATGQWCPNPAGCPETMSHVVSMIRAADADIVGLQEGTANECVIADRLGWHCEPRLQ
                     LISRYPLLDPPGADGAYALAEVTPGRFVAISNVHLPSDPYGPYWVRDGEPRAAVLDLE
                     RTTRLPAIQRELDVLPELADTGMPVFLTGDFNSPSHLDWTPAVDAVREEVRYPVVWPV
                     SAALADAGFRDSYREVHPNPVAVPGFTWTPPGTLESVPDEVHDRIDWVLVQGPAETLA
                     TKLVGEAGGPDVDIERDPYPTDHRGVVSTFRVDAGDPAPFAAAGARRVFVGSDLDVRA
                     HGPGSRVVLVRAGQGPAAGLVSRSLGDASDVTLTFPTATLSPGAHEALLVNASDAVLS
                     RSPFWVYAAGTPPSLRTSKEVYEVGEPIRVRWRAAPGYRFDWLGVFSPGRGPAQNAED
                     CNAGTCGNGHYLLYDYTDTLIQGSTLIGPASFAGYRTWPLKVGTYEIRLLMDDGYRLL
                     GISNTLKIVHAAG"
     gene            2047..2931
                     /locus_tag="VF036_01155"
     CDS             2047..2931
                     /locus_tag="VF036_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6263868.1"
                     /translation="MSPIVIIVLVVVALIVVAYLAGLRPAPVSGRRPTPGLLRRRSKW
                     RKVLPFLPLLAAVGCLVYAFSGFRLDVEEASPVIVLVLDVSDSMNATDVAPDRLTAAE
                     TAARAFLDELPPDFRVGLTTFASGAQLVVRPTQVREEVVDALVGLTTSKGTVIGDGLT
                     VALDAIEELRSGTPDTPAAALLLSDGHDTGSRVTPGQAAARAAGLEVPVYTVVVGRVP
                     EGEGPGADVGALESIANTSGGATYTAETSAELTSIYAELGSTLSVNLEVAPSTTPFVI
                     AAIALTVLAGLLLVYLPR"
     gene            3048..4076
                     /locus_tag="VF036_01160"
     CDS             3048..4076
                     /locus_tag="VF036_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014442387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6263869.1"
                     /translation="MQDLTLERTDDVGAEALPSARGPLSATMLGLLTDGNVGRHPARV
                     PDDPLGDDDLHLALCLAYELSYRGLPGVRDDLEWDLPVLGARRDLEEAFEAGLRHTIG
                     RESVAPRDVPDRLREIATPPDADSLSTFIEREATFDQAREFLMHRSAYQLKEADPHSW
                     VIPRLDGASKTAVMEIQYDEYGSGDPAWMHSELFRTTMIAVGLDGSYGAYLDRLPGPT
                     LATVNLITLFGMHRRWRGALVGHLAAFEMTSSLPNRAYAQGFRRLGFDDDAVRFFDEH
                     VEADSVHEVLAGDLAAGLAAEEPELATDVVFGAAAMALLEGRFADGLLRAWRAGDSTL
                     LPAGVRPW"
     gene            4073..>4506
                     /locus_tag="VF036_01165"
     CDS             4073..>4506
                     /locus_tag="VF036_01165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6263870.1"
                     /translation="MSPDLSYILPYRSRPGDGNEEELDRYVRWLSLRVQLIVVDGSDP
                     DRIRAHQERWASIATHTVPDADVRCLNGKVRGVLTGLRRARHEHVIIADDDVRYDDEA
                     LLRMQELLRNSDLVRPQNFFDPIPWHAHWDTSRMLLNRALGN"
BASE COUNT          628 a         1632 c         1535 g          711 t
ORIGIN      
        1 cgaggcggcg ccctcgatcg cgaccccgat gaagagcacg atgccgatcc cgatcaggag
       61 gacacgcttg tcgtgctctc cgaacgtccg gatcgcgaac gacttgagcc actcgggcgt
      121 cgcgtcgacc gcggcgccac ccacggcgac gatgggggag gaccccgggc cgaagaccgc
      181 ggacagcagc tccgccgttc cgagcgccac cgcggcggcg agcacgccga tcgtcgcccc
      241 cacgccacgg cggatctcgg atgtcgcctg ttccgctccc acgcccacct gtgtctccat
      301 ccaggcatga cgttactccc ggacccgggc cggtctgttc gagacggacc cggccggata
      361 ggctcttccg atcatccccc cgaagggaga agtggatgcc gcgacgcttg ctcgccctgg
      421 tcagctcgct cggtctcgtg ctcctcctgt tggccccgac ggcccgagcg gccccgatca
      481 ccgtgaaggt catgaccctg aacgtcttct atggcggtga cgagtggaac ctcgcgacgg
      541 gccagtggtg cccgaaccct gccggctgtc ccgagaccat gagccacgtc gtctcgatga
      601 tccgggcggc cgacgccgac atcgtcggtc tccaggaggg cacggcgaac gagtgcgtga
      661 tcgccgatcg cctcggatgg cactgcgagc ctcggctcca gctgatctct cgctacccgc
      721 tcctcgaccc ccccggggcc gacggcgcgt acgcgctcgc cgaggtcacc ccgggacggt
      781 tcgtcgcgat ctcgaacgtc catctgccgt ccgatccgta cggcccgtac tgggtccgcg
      841 acggagagcc acgcgcggcc gtgctcgacc tcgagcgcac gacgcgcctg ccggcgatcc
      901 agcgggagct cgacgtgctc cccgagctcg ccgacaccgg catgcccgtg ttcctcaccg
      961 gggacttcaa ctccccatcc catctcgatt ggacgccggc ggtcgatgcc gtccgcgagg
     1021 aggtccgcta cccggtcgtc tggccggtct cggcggccct cgccgatgcg ggcttccgtg
     1081 attcgtaccg cgaggtgcac ccgaaccccg tggccgtccc cgggttcacc tggacgccac
     1141 ccgggacgct cgagtcggtg cccgacgagg tgcacgaccg gatcgactgg gtgctcgtgc
     1201 agggtcccgc cgagacgctc gccacgaagc tggtcggcga ggccggcgga ccggacgtcg
     1261 acatcgagcg ggatccgtat ccgaccgacc accgcggcgt ggtgtcgacg ttccgcgtgg
     1321 acgccggcga ccccgcgccg ttcgcggcgg cgggcgcgcg cagggtgttc gtcggcagtg
     1381 atctggatgt gcgtgctcac gggccgggtt cgcgcgtggt gctcgttcgc gcgggtcagg
     1441 gcccggccgc ggggctggtc tcgcgctccc tcggcgacgc gtccgacgtc accctcacgt
     1501 tcccgacggc aacgttgtca ccgggtgccc acgaggcgct gctcgtgaac gcgagcgacg
     1561 ccgtgctgtc ccggagcccg ttctgggtct atgcggccgg gacgccaccg agcctgcgga
     1621 cctcgaagga ggtctacgag gtcggggagc cgatccgcgt gcggtggcgg gccgcacccg
     1681 ggtatcgctt cgactggctg ggcgtgttct cgcccggacg aggaccggcg cagaacgcgg
     1741 aggactgcaa cgcggggacc tgcggcaacg gccactacct cctctacgac tacacggata
     1801 cgctgatcca agggtccacg ctgatcggcc ccgcttcctt cgccgggtac cgcacgtggc
     1861 ccttgaaggt gggtacgtac gagatccgcc tcctcatgga cgacggctac cgcctcctcg
     1921 ggatctccaa cacgttgaag atcgtgcacg ccgcggggta acggctgccc gcgtggcggc
     1981 gtccccgacc gaggagatcg gggtggccgc cgcgctcgta gccttcgcgc tacggttggc
     2041 ggcgagatgt ctcccatcgt gatcatcgtg ctcgtcgtgg tggcgctgat cgtggtggcg
     2101 tatctcgccg gcctccgacc cgcccccgtc agcggtcgca ggccgacccc cgggctcctg
     2161 cgccgtcgct cgaaatggcg gaaggtcctc cccttcctgc cgctcctcgc ggccgtcggc
     2221 tgcttggtct acgcgttcag cgggttccgt ctcgatgtcg aggaggcttc cccggtgatc
     2281 gtgctggtgc tcgacgtctc cgactcgatg aacgccaccg atgtcgctcc ggaccggctc
     2341 accgccgccg agaccgcggc gagggcgttc ctcgacgagc tccctccaga cttccgggtc
     2401 ggattgacga cgttcgccag cggtgcacag ctcgtcgtgc gccccacgca ggtccgagag
     2461 gaggtggtgg acgcgttggt gggcctcacc acgtcgaagg gcaccgtgat cggcgacggg
     2521 ctcacggtgg cgctggatgc gatcgaagag ctccggtccg ggaccccgga cacgccggcg
     2581 gccgccctcc tcctgtccga cggccacgac accgggagcc gggtcacgcc cggccaggcc
     2641 gcggcacgag cggctggcct cgaggtcccg gtgtacacgg tggtcgtcgg ccgggtgccc
     2701 gaaggcgagg gccccggcgc cgacgtgggc gcgctcgagt cgatcgcgaa caccagcggc
     2761 ggtgccacgt acacggccga gacgagcgcc gagctcacct cgatctacgc ggagctcggt
     2821 tccaccctgt cggtgaacct cgaggtcgcg ccgagcacca cccccttcgt gatcgccgcg
     2881 atcgcgctca ccgtgcttgc gggactcctc ctcgtctacc tgccgcgctg atcggcggtc
     2941 cacggatccg ttccggcgaa cggcacctgg cgggtggtcg gagccgcgtg agcgcccacc
     3001 cgagccggca ggggtaggaa cctcctcgct cgggtatccc ttcggcgatg caggatctga
     3061 ccctggaacg gacggacgat gtgggcgcgg aggcgctccc gtccgcgcgg ggaccgctga
     3121 gcgccacgat gctcggtctc ctcaccgacg ggaacgtggg gcggcatccg gcgcgggtcc
     3181 cggacgatcc cttgggggac gacgacctcc atctggcgct ctgcctcgcc tacgagctct
     3241 cctatcgagg cctgcccggc gtgcgtgacg acctcgagtg ggatcttccg gtcctcggcg
     3301 cgcgtcgcga cctcgaggag gcgttcgagg ccgggctgcg tcacacgatc gggagggagt
     3361 ccgtcgctcc gcgcgatgtc cccgaccggc tccgggagat cgccacgcca ccggatgccg
     3421 actcgctctc cacgttcatc gagcgggagg caaccttcga ccaagcccgc gagttcctga
     3481 tgcatcgctc ggcctaccaa ctcaaggaag cggatccgca ctcctgggtg atcccacgcc
     3541 tggacggcgc ctcgaagacg gccgtcatgg agatccagta cgacgagtac ggcagtggcg
     3601 acccagcgtg gatgcactcc gagctcttcc gcaccacgat gatcgcggtc ggtctcgatg
     3661 gctcctacgg tgcctacctc gaccggctcc cggggccgac cctcgcgacc gtgaacctga
     3721 tcacactgtt cgggatgcac cgccgatggc gaggagccct cgtcggccac ctcgcggcgt
     3781 tcgagatgac ctcgagcctg ccgaaccgcg cgtacgcgca agggttccga cggctcgggt
     3841 tcgacgacga cgcggtgcgg ttcttcgacg agcacgtcga ggccgactcc gtccatgagg
     3901 tcttggccgg cgatctggcc gccggcctcg ctgcggagga gcccgagctc gcgaccgacg
     3961 tcgtcttcgg agcagccgcg atggcgctgc tcgaaggtcg tttcgcggac ggtctgttgc
     4021 gagcgtggcg ggccggcgac tcgacgctcc tcccggccgg cgtgcggccg tggtgagccc
     4081 ggatctctcc tacatcctcc cgtaccggag ccgtcccggg gacgggaacg aagaggagct
     4141 cgatcggtat gtccgatggc tgtccctgag ggtccagctg atcgtcgtcg acggctcgga
     4201 ccccgaccgt atccgtgcgc atcaggagcg gtgggcctcg atcgccaccc ataccgtgcc
     4261 cgatgcggat gtcagatgcc tgaacggcaa ggtccgaggc gtcctcaccg ggctgcgccg
     4321 agcccggcac gagcacgtga tcatcgccga cgacgacgtt cgctacgacg acgaggctct
     4381 gctccggatg caggagctcc ttcggaactc cgacctcgtc cggccacaga acttcttcga
     4441 cccgatcccg tggcacgccc actgggacac gtcgcggatg ctgctcaacc gggcgctcgg
     4501 gaacga
//
LOCUS       DASUVE010000056         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_781463, whole genome shotgun sequence.
ACCESSION   DASUVE010000056 DASUVE010000000
VERSION     DASUVE010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781463"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1068
                     /gene="ccsB"
                     /locus_tag="VF036_01170"
     CDS             118..1068
                     /gene="ccsB"
                     /locus_tag="VF036_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEX6263871.1"
                     /translation="MNAARLSSAAFDLTLWLYLAAMVLSFAYLAARRDLLWKVARAVA
                     IAGATANLVSIVARGVAAQRVPWGNMFEYSTVLAFLVVVGTLVFVEGVAKVKTLTGFS
                     LMFSVLTLAIAAIFFPVEPSPLQPALNSYWIKIHVVAAITGSSLFAIGGIATILFLIK
                     DRSERRAPSVLRDRTPPPIMGGAIEVDAPHDLLPDPDGSGTPAPESARGGVLPSAAAL
                     DRVAYRVIAFAFPIWTFAVIAGAIWAQDAWGRYWGWDPKETWSFVTWTIFAGYLHARS
                     TAGWKGRRAAVIAVVGFVSLLITYYAVNLWIVGLHSYKNG"
     gene            1158..2261
                     /gene="coxB"
                     /locus_tag="VF036_01175"
     CDS             1158..2261
                     /gene="coxB"
                     /locus_tag="VF036_01175"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX6263872.1"
                     /translation="MRKRLLWATAGIGLLLLLSGCAPDATQDTLQPQGPYAQQLKDLF
                     IPVFWVAVFVFIVVEGGIVWIAIRYRHRKGRERMPAQIHGNTRLEIGWTIAPALILAG
                     VMVPTVATIWDLAKKPPDDALHVTVEGFQWWWGFTYTDDDMQTNSGDNLPIRTADVLV
                     IPEDRVVYLSLEAAGGEIQGNDPAASDYVVIHSFWIPELAGKQDVVPGRTNHILMQAD
                     DPGVYTGQCAEFCGLQHGRMLVRVVALSAEDWDAWVENQKRTAATPSDPLARQGMDLF
                     LEPLSGGRGSCINCHAVAGTDAVSQAGPELTHFADPTHECFAGCNWEVKDREALAAWL
                     RDPNDVKLGSKMPDYDLTEQEIEALMAYLYSLT"
     gene            2293..>2712
                     /locus_tag="VF036_01180"
     CDS             2293..>2712
                     /locus_tag="VF036_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX6263873.1"
                     /translation="MASVTTTRGREGTLRRPTAVTGFSSWFTTIDHKKIGILYGVTAF
                     VFFIVGGVEALLIRAQLAQPDGTLLNADAYNQIFTMHGITMIFLFVMPMAAAFFNYFI
                     PLMIGARDVAFPRLNAYSYWVFLLGGLFLYSSFFLGGA"
BASE COUNT          419 a          894 c          929 g          470 t
ORIGIN      
        1 acggaaggac tccttcgagg acgagttcga tgcgatcgtg cgggccgtcg gcggtcgtcc
       61 gtctgccccg cagcgaacct ccgaacgacc gggtccggag gagcgggtgg gaacgtcgtg
      121 aacgcggcga ggctctcgag cgcggcgttc gacctgaccc tgtggctcta cctcgccgcg
      181 atggtcctgt cgttcgccta cctcgcggcc cggcgcgacc tgctctggaa ggtggctcgc
      241 gcggtcgcga tcgcgggcgc cacggccaat ctcgtgtcga tcgtggcgcg gggggtggcg
      301 gcgcagcgcg tgccctgggg caacatgttc gagtactcga cggtgctcgc gttcctcgtg
      361 gtggtgggca cgctcgtctt cgtcgagggc gtcgcgaagg tcaagaccct cacgggcttc
      421 tcactgatgt tctcggtgct cacgttggcg atcgcggcga tcttcttccc ggtggagccg
      481 agcccattgc agccggcgct caactcgtac tggatcaaga tccacgtcgt cgcggcgatc
      541 accggttcgt cgctgttcgc gatcggcggg atcgcgacga tcctgttcct catcaaggat
      601 cgctcggagc gccgggcacc ctcggtcctc cgcgatcgca cgccgccgcc gatcatgggt
      661 ggcgcgatcg aggtcgatgc gccacacgac cttctcccgg atccggatgg atccggcacg
      721 ccggctcccg agtccgcgcg cggcggtgtc ctcccgtcgg ccgcggcact cgaccgagtg
      781 gcctaccgcg tgatcgcgtt cgcgttcccg atctggacgt tcgccgtgat cgccggggcg
      841 atctgggcgc aggacgcgtg ggggcgctac tggggatggg acccgaagga gacgtggtcg
      901 ttcgtcacgt ggacgatctt cgcggggtac ctgcacgcgc gttccacggc cgggtggaag
      961 ggccgccgcg ccgccgtgat cgccgtcgtc ggcttcgtct cgctcttgat cacctactac
     1021 gccgtgaacc tgtggatcgt cggattgcac tcgtacaaga acggatgagg cgtcaagtgc
     1081 cgcgagaccc cggtgataca gtgcgcgcgg gctcgccgag cccgtcctca gaagagctcc
     1141 gggagaccga aggaaccgtg cggaagaggt tgctctgggc gacggccggg atcggcctcc
     1201 tcctgctgtt gagtggctgt gcacccgacg ccacccagga cacccttcag ccgcaggggc
     1261 cgtacgccca gcagctgaag gacctcttca tccccgtctt ctgggtcgcg gtcttcgtct
     1321 tcatcgtcgt cgagggcggc atcgtgtgga tcgcgatcag gtaccggcac cgcaagggcc
     1381 gcgagcgcat gccggcgcag atccacggca acacgcgcct cgagatcggc tggacgatcg
     1441 ctccggcgct gatcctcgcc ggcgtgatgg tgcccaccgt cgcgacgatc tgggacctcg
     1501 ccaagaagcc gccggacgac gcgctccacg tcaccgtcga gggcttccag tggtggtggg
     1561 gcttcacgta cacggacgac gacatgcaga cgaactccgg ggacaacctt ccgatccgga
     1621 cggccgacgt gctggtgatc cccgaggacc gtgtcgtcta cctctcgctc gaagccgccg
     1681 gcggagagat ccaggggaac gatcccgccg cctcggacta cgtggtgatc cactccttct
     1741 ggatcccgga gctggcgggc aagcaggacg tggtacccgg ccggacgaat cacatcctga
     1801 tgcaagcgga cgaccccggc gtgtacacgg gccagtgcgc cgagttctgc ggtctccagc
     1861 acggacggat gctcgtgcgg gtggtcgcac tctcggccga ggactgggac gcatgggtcg
     1921 agaaccagaa gcgcaccgcg gccacgccga gcgatccact cgcccggcaa gggatggacc
     1981 tgttcctcga gccgctctcg ggtggccgtg ggtcctgcat caactgccac gccgtggccg
     2041 ggacggatgc cgtgagccaa gccgggcccg agctgaccca cttcgccgac ccgacccacg
     2101 agtgcttcgc ggggtgcaac tgggaggtga aggaccgcga ggcgctggcg gcctggctcc
     2161 gcgatccgaa cgatgtgaag ctgggctcga agatgccgga ctacgacttg accgagcagg
     2221 agatcgaggc cctcatggcg tacctctaca gcctgacatg agaacggcgt gcgacgacgg
     2281 agcggatgac cgatggcgag cgtgacgacg acccgcggac gcgaaggcac cctgcgacga
     2341 ccgacggcgg tgaccggctt ctcgagctgg ttcacgacaa tcgaccacaa gaagatcggg
     2401 atcctgtacg gggtgacggc cttcgtgttc ttcatcgtcg ggggtgtcga ggcgctgctg
     2461 atccgcgccc agctcgcgca gcccgatgga acgctgctca acgccgatgc gtacaaccag
     2521 atcttcacca tgcacgggat cacgatgatc ttcctgttcg tgatgccgat ggcagcggcg
     2581 ttcttcaact acttcatccc gctgatgatc ggtgcgcggg acgtcgcgtt cccacggttg
     2641 aacgcgtaca gctactgggt gttcctgctc ggcggcctgt tcttgtactc gagcttcttc
     2701 ctcgggggcg cg
//
LOCUS       DASUVE010000057         3482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_594053, whole genome shotgun sequence.
ACCESSION   DASUVE010000057 DASUVE010000000
VERSION     DASUVE010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594053"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /gene="hisD"
                     /locus_tag="VF036_01185"
     CDS             complement(<1..918)
                     /gene="hisD"
                     /locus_tag="VF036_01185"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX6263874.1"
                     /translation="MARYLKERTGVAEETWSEIRATVSEILGAVEREGEPAVRRYSDR
                     FDGWSPPSFRVSEDAIRDAADAISDELREHIAFAQTQIRGFAELQRSTLVDAEWETLP
                     GIMLGQRFVPVNAVGAYSPGGLYPLIASAIMTVLVPKVAEVARVVVCAPPRGPAGMHP
                     PQLYAMVTSGADEILCIGGVQAFAAMAFGIEDLEPVDMLVGAGNAYVAEAKRQLFGTV
                     GIDLLAGPTEVMVLADDSADPEIVAADLLGQAEHGPTSPATLVTTSEDLARAVSDAVE
                     AWLDGEWPTKEVAGTAWRDHGAIIVCEDEE"
     gene            1024..2088
                     /locus_tag="VF036_01190"
     CDS             1024..2088
                     /locus_tag="VF036_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6263875.1"
                     /translation="MAVVDYGKPLELIDLPEPELRPGFALLEVISCGVCFSDVKTSRG
                     RMPFSDDLRFPHVPGHEIYGRVIASDPEEAIAEGTRAVVYHYWPCGTCSACRRGDETL
                     CRSMVGWAGFTHPGGFTERMTVPVGRLVPIPESIDPIHAAPMSCALGTAYRSVVTRGG
                     AGAGMTAAIVGLGGVGIHAAQMGRAAGSRTAGFDVHEPTLAAARDLELQVWRADDDAA
                     IDALIGSTDGEGVDVVVDTVGHDDTLELARRLVRPGGRVVVVGYTPTSALSVPTPRLV
                     LDEVDYRGSRYAHRDDLAKAVALVERGLVSTVVGMVRPLEAVNDVFDELEAGAVVGRA
                     VLEVADIPSARNPSDIVSAG"
     gene            2088..2759
                     /locus_tag="VF036_01195"
     CDS             2088..2759
                     /locus_tag="VF036_01195"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6263876.1"
                     /translation="MAYRQDALGRDTTAKVVAERLRDEIQHGTLAPGTRLRQNDVAER
                     FGVSTTPVREAFAQLQAEGLVRIDPHRGAVVFHPSVDDVLEFYEIREALESVAVAHAI
                     PNLRKDVARDLGALIDRMRRTEDARKWLRLNDQFHLRLYEGAARPRLSGLIDSLRDAS
                     APYIYMFVASRPPSQRANEQHQEILDACVRGDAAGAKRAIREHLRSAARDLAAFLAAQ
                     ESTTP"
     gene            2934..>3482
                     /locus_tag="VF036_01200"
     CDS             2934..>3482
                     /locus_tag="VF036_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6263877.1"
                     /translation="MRTRSLVRLVAATCALALIAAACGEDGGTGGEGERSIVRFAFSP
                     DPVIDWMNDQGIIPEYEEQYNVRLVTTSTWDEFTFFAGGHGDIVSMATYETPLLEQET
                     GVDTVTFGSYNNLRVPVFVRSDSDYETFEDLQGTKVAVPSAVSSTIVWGMFVKDWYGL
                     DFCIERPDGSDCADYEVIEGDHF"
BASE COUNT          550 a         1192 c         1191 g          549 t
ORIGIN      
        1 ctcctcgtcc tcgcacacga tgatcgcccc gtgatcacgc cacgcggtac cggcgacctc
       61 cttcgtgggc cactcgccat cgagccatgc ctcgacggcg tcgctcacgg cgcgagcgag
      121 atcctccgac gtcgtgacca gcgtggcggg cgacgtcgga ccgtgctcgg cctgtccgag
      181 aaggtccgcg gcgacgatct ccggatcggc ggagtcgtcc gccagcacca tcacttcggt
      241 cggccccgcc aggaggtcga tccccacggt gccgaacagc tgacgcttcg cctccgcgac
      301 gtacgcgttc ccggccccga ccagcatgtc cacgggctcg aggtcttcga tgccgaacgc
      361 catcgcagcg aacgcttgta ctccaccgat gcagaggatc tcgtcggctc cggacgtcac
      421 catcgcgtag agctgaggcg gatgcatccc ggccggcccg cgcggcggcg cgcacaccac
      481 cacgcgcgcc acctccgcca ccttgggcac gagcacggtc atgatcgcgg aggcgatcaa
      541 cggatagagg ccgcccggcg agtacgcgcc aacggcgttt accggaacga accgctgtcc
      601 gagcatgatg ccgggcaggg tctcccactc cgcgtcgacc agtgtcgatc gctgcagttc
      661 cgcgaaccca cggatctgcg tctgcgcgaa cgcgatgtgc tctcgaagct cgtcgctgat
      721 cgcatcggcg gcatcgcgga tagcgtcttc ggagacgcgg aacgacggtg ggctccaccc
      781 gtcgaaccgg tcggagtaac ggcgcaccgc cggctcgcct tcgcgttcca ccgcgccgag
      841 gatctcgctg acggtcgcgc ggatctcgga ccacgtctcc tcggcgaccc cggtccgctc
      901 cttgaggtat cgagccatcg cacactcctc tcggatcggc tccgtgcccg aacagttcgt
      961 atattatatg taggcaacgc gccggggtca cccggctcga tcgcggagga gcccatgcgc
     1021 gcgatggctg tcgtggacta cgggaagccg ctggagctca tcgacctgcc cgagcccgag
     1081 ctccggccgg gcttcgcatt gctggaggtc atctcctgcg gcgtctgctt cagcgacgtc
     1141 aagacctccc gcgggcggat gccgttcagc gatgacctcc ggttcccgca cgtccccggc
     1201 cacgagatct acgggcgggt gatcgcgtcc gatcccgagg aggcgatcgc ggaaggcacc
     1261 cgcgccgtcg tctaccacta ctggccgtgc gggacctgct ccgcgtgtcg acgcggcgac
     1321 gagacgctct gccgcagcat ggtcggttgg gcgggtttca cccatcccgg cggcttcacc
     1381 gaacggatga cggtcccggt gggtcgactg gtgccgatcc cggagtcgat cgacccgatc
     1441 cacgccgcgc cgatgtcgtg cgcgctgggg acagcctatc gctccgtcgt caccagaggt
     1501 ggcgccgggg ccgggatgac ggccgcgatc gtcgggctgg gcggtgtcgg gatccacgca
     1561 gcgcagatgg gacgagccgc gggctcccgt acggccgggt tcgacgtcca cgagcccacg
     1621 ctcgcggcgg ctcgagacct cgaactgcag gtttggcggg ctgacgacga cgcggcgatc
     1681 gacgccctga tcggcagcac ggacggtgaa ggcgtcgacg tggtcgtcga cacggtggga
     1741 cacgacgaca ccctcgaact cgcccgacgg ttggttcgtc cgggcgggcg ggtcgtcgtc
     1801 gtcggataca cgcccacgtc cgcgctctcc gttccgacgc cgaggctcgt cctggacgag
     1861 gtggactaca gggggtcacg ctacgcgcac cgggacgacc tcgcgaaggc cgtcgcactc
     1921 gtcgagcgtg gcctcgtatc gaccgtggtg gggatggtcc gacccctcga agcggtgaac
     1981 gacgtgttcg acgagctcga ggccggcgcc gtcgtcggcc gcgcggtcct ggaggtcgcc
     2041 gatatcccga gtgctcgcaa cccgtccgat atcgtgagcg ccggctgatg gcgtatcgac
     2101 aggacgcgct cgggcgggat accactgcga aggtcgtcgc ggagcggctc cgcgacgaga
     2161 tccagcacgg cacgttggcc cccggaaccc ggctgcggca gaacgacgtc gcggagcggt
     2221 tcggcgtgag cacgacaccc gtccgtgagg cgttcgcgca gctgcaggcg gaggggctgg
     2281 tcaggatcga cccgcatcgc ggagctgtcg tgttccatcc gagcgtcgac gacgtgctcg
     2341 agttctacga gatccgcgag gcactggagt ccgtcgccgt cgcacatgcg atcccgaatt
     2401 tgcgcaagga tgtggctcgc gacctcggag cactgatcga tcggatgcgg aggaccgagg
     2461 atgccaggaa gtggctcagg ctgaacgatc agttccatct gcgtctctac gaaggtgccg
     2521 cgcggcctcg cctctcgggc ctgatcgata gcctgcgcga cgcctccgct ccgtacatct
     2581 acatgttcgt ggcctcgagg cccccgtcgc agcgggccaa cgaacagcac caggagatcc
     2641 tggatgcgtg cgtccgcggc gatgcggcgg gcgcgaagcg ggccatccgc gaacacttgc
     2701 gaagtgccgc ccgcgacctg gccgcgttcc tcgccgccca ggaaagcacc accccctagc
     2761 gcgacccggg accgtctgca ccgccttgac ggtcggcgac aagactgcgc attatatata
     2821 gtccgctgga aaggtggggg acgcggaacg gcgacccggc cccatgggat cccccaccgc
     2881 ggcctcgttc ctggccgtgc gagtcccgat gggtcccgac gagggagggg cggatgcgga
     2941 cgaggagtct ggtacgactc gtggcggcga catgcgcgct cgcgctcatc gcggcggcgt
     3001 gcggtgagga tggggggacg ggaggcgaag gcgagcgatc gatcgtccgc ttcgcattct
     3061 ctccggaccc ggtgatcgac tggatgaacg accaggggat catcccggag tacgaggagc
     3121 agtacaacgt ccggctggtg acgacgtcca cgtgggacga gttcacgttc ttcgccggcg
     3181 ggcacggaga catcgtgtcg atggcgacct acgagacccc gctcctcgag caggagaccg
     3241 gcgtcgacac cgtcacgttc ggctcgtaca acaacctgcg cgtccccgtc ttcgtccggt
     3301 cggactccga ctacgagacg ttcgaggacc tgcagggtac gaaggtcgcc gtcccgagcg
     3361 cggtctcctc gacgatcgtg tggggcatgt tcgtcaagga ctggtacggc ctcgacttct
     3421 gcatcgagcg tcctgacggg agcgactgcg ccgactacga ggtgatcgag ggcgaccact
     3481 tc
//
LOCUS       DASUVE010000058         3689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_970317, whole genome shotgun sequence.
ACCESSION   DASUVE010000058 DASUVE010000000
VERSION     DASUVE010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970317"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VF036_01205"
     CDS             <1..799
                     /locus_tag="VF036_01205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008717027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX6263878.1"
                     /translation="REIPLQLVDLDIGILWVLAMTSIAVYGVVLAGWASGSNYPLLGA
                     VRSTAQMISYEVAMGLSLVAVLMYSGHLSLSEIVAAQDRVWNVVPMFPAFVVYLICGI
                     AETNRPPFDLAEAETELVAGFHTEYSGITFAMFFLGEYVATVTVAAIGVTLFLGGWRG
                     PAPDFLPGLWPFLWFLLKVTLVIYLFVLIRGTLPRVRYDRLMAFGWKVLIPFGLVWVL
                     VTGAIVVLPTELELDRGAVLTWAGVALGATLLLSFLPFGRASKEVPR"
     gene            796..1296
                     /gene="nuoI"
                     /locus_tag="VF036_01210"
     CDS             796..1296
                     /gene="nuoI"
                     /locus_tag="VF036_01210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX6263879.1"
                     /translation="MTDEPRRERNFLADTGAVLKGMATVFKQTFRPDDTEEYPKVIAE
                     IPERAHTGRHRLNRHDDGLEKCIGCELCAFACPADAIWVEGEDNDPEHPTSPGERYAK
                     DYQINYLRCIMCGLCVEACPTRALTLTSYFEMAYTSREDAILTKEQLLEPPPELGTTP
                     GAKEGL"
     gene            1299..1853
                     /locus_tag="VF036_01215"
     CDS             1299..1853
                     /locus_tag="VF036_01215"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX6263880.1"
                     /translation="MPLDNVVFWVFAPISVASAIAMLLMRNAVHAALFLVVNFFCLAV
                     FYLILDAPFLFAVQIIVYAGAIMVLFLFVIMLLGVDRGDDLRERLVAQRPLALALAAA
                     FVIEVTLAVRAGIGFATKAPDGFDAVNDPGNAQAVARVLFRDYFLPFEVTSVLLIIAA
                     VATMVLGHRKARAVTQAELEAMRG"
     gene            1873..2178
                     /gene="nuoK"
                     /locus_tag="VF036_01220"
     CDS             1873..2178
                     /gene="nuoK"
                     /locus_tag="VF036_01220"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019435768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX6263881.1"
                     /translation="MRTPIAYFVVLSGVLFSLGTLGVLLRKNALLIFMSIELQLNAVN
                     LALVAFSRMHVALDGQVLAFFSMVVAAAEVVVGLAIIVAVYRRRGTVDVDDARGLAG"
     gene            2223..>3689
                     /gene="nuoL"
                     /locus_tag="VF036_01225"
     CDS             2223..>3689
                     /gene="nuoL"
                     /locus_tag="VF036_01225"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX6263882.1"
                     /translation="MWLIPAIPLLGAGINLFAGRRLRKSAGWLASAAVTTSFLLAVWA
                     VVELMGAREHERLVIQHLFDWISVGRFSVGADLRLDVLSATMILVVTGIGLLIHVYAI
                     GYMEGDPRYGRFFAYLNLFVFFMLLLVLADNYLLLYVGWEGVGLCSYLLIGFWFEKTE
                     NAVAAKKAFVTTRIGDTLMLIGLALIVAKFGSLDFTVVLGTPGETIAKGTATAISLLL
                     FAGAIGKSAQVPLHVWLPDAMAGPTPVSALIHAATMVTAGVYLVVRSAPLFELSGTAL
                     TVVLIVGLVTMLFAATCALAQDDIKRVLAYSTISQLGFMFMAAGMRFYTGAMFMLVAH
                     ASYKALMFLGAGSVMHGMREETDLQRMGGLIRRMPVTGWTFVVGAFALAGVFPLAGFF
                     AKDQILEFANRTGRTWIYLLGTLGALMSALYIGRLLFLAFFGRPRSEEAEHAHESPAV
                     MTVPLAILAVGAIVAGLLLSTSAEGTLARVLEPVVGSLP"
BASE COUNT          484 a         1209 c         1277 g          719 t
ORIGIN      
        1 ccgggagatc ccgctgcaac tcgtcgacct cgacatcggc atcctctggg tgctcgcgat
       61 gacctcgatc gcggtctatg gggtggtgct cgcgggatgg gccagcggat cgaactaccc
      121 gctcctcggc gccgtgcgct cgaccgcgca gatgatctcc tacgaggtgg cgatgggcct
      181 ctcgctcgtc gccgtgctga tgtacagcgg ccatctctcg ctctcggaga tcgtcgccgc
      241 gcaggatcgg gtgtggaacg tggtgccgat gttcccggcg ttcgtggtct acctgatctg
      301 cgggatcgcc gagacgaatc ggccgccgtt cgatctggcc gaggccgaga cggagctcgt
      361 ggcggggttc cacaccgagt actcgggcat cacgttcgcg atgttcttcc tcggcgagta
      421 cgtcgcgacc gtgaccgtcg cagccatcgg cgtgacgctg ttcctcggcg gatggcgtgg
      481 cccggcgccg gacttcctcc ccgggctctg gccgttcctg tggttcctgc tgaaggtcac
      541 gctcgtgatc tacctgttcg ttctgatccg cggcacgctt ccgcgggtcc gctacgaccg
      601 actgatggcg ttcggctgga aggtcttgat ccccttcggc ctcgtctggg tgctcgtcac
      661 gggcgcgatc gtggtgcttc ccacggagct cgagctcgac cggggggccg tgttgacgtg
      721 ggccggggtt gccctcggcg cgacgctgct cctgtcgttc ctgcctttcg gccgcgcgtc
      781 gaaggaggtg ccccgatgac ggacgagccg cgccgggagc ggaacttcct cgcggacacg
      841 ggcgccgtgc tgaagggcat ggcgacggtg ttcaagcaga cgttccgtcc cgatgacacc
      901 gaggaatacc cgaaagtgat cgcggagatc ccggaacgcg cgcacaccgg tcgccaccgg
      961 ctgaaccggc acgacgacgg cttggagaag tgcatcgggt gcgagctctg cgcgttcgcc
     1021 tgtcccgccg acgcgatctg ggtggaaggg gaggacaacg atcccgagca cccgacctcg
     1081 cccggggagc gctacgcgaa ggactaccag atcaactacc tgcgctgcat catgtgcggg
     1141 ctctgcgtcg aggcctgccc gacccgcgcg ctgaccctga ccagctactt cgagatggcg
     1201 tacacctcgc gtgaggacgc gatcctcacg aaggagcagc tcctggagcc gcccccggag
     1261 ctcggcacca ccccgggggc gaaggagggt ctgtaggggt gccgctcgac aacgtcgtgt
     1321 tctgggtgtt cgcgccgatc tcggtggcgt ccgccatcgc gatgctgctg atgcggaacg
     1381 cggtccacgc ggcgctgttc ctcgtcgtga acttcttctg tctcgcggtc ttctatctga
     1441 tcttggacgc gcccttcctg ttcgccgtcc agatcatcgt atacgcgggc gcgatcatgg
     1501 tgctgttcct gttcgtgatc atgctgctcg gcgtcgaccg gggtgacgat ctccgcgagc
     1561 ggctcgtggc gcaacggccg ctcgcactcg cgctcgccgc cgcgttcgtg atcgaggtca
     1621 ccctcgcggt gcgggcgggc atcgggttcg ctacgaaggc tccggacggg ttcgacgcgg
     1681 tgaacgatcc cggcaacgcg caggccgtcg cgagggtcct gttccgcgac tacttcctcc
     1741 cgttcgaggt cacatcggtc ctgttgatca tcgcggcggt cgcgacgatg gtactggggc
     1801 atcggaaggc ccgcgcggtg acccaggcag agctggaggc gatgcgcgga tgagatcgat
     1861 gcaggcgggc cggtgaggac gccgatcgcc tacttcgtgg tcctgtcggg cgtgctgttc
     1921 tcgctcggga ccctcggcgt gctgctccgg aagaacgcgc tgctgatctt catgtcgatc
     1981 gagctccagc tcaacgccgt gaacctcgcg ctcgtggcgt tctcgcggat gcacgtcgcg
     2041 ctcgacggcc aggtgcttgc gttcttctcg atggtggtgg cggcggcgga ggtagtcgtg
     2101 ggtctcgcga tcatcgtggc ggtgtaccgg cgtcgcggga ccgtcgacgt cgacgacgcg
     2161 agagggctcg caggctgatg ctcgcgcagg tcggtggtcc tccggcccca gccctcgggt
     2221 ggatgtggct tatccccgcg atcccgctcc tgggtgccgg gatcaacctc ttcgccggcc
     2281 gacgcctccg gaagagcgcg ggctggctgg cttcggccgc cgtgaccacg tcgttcctcc
     2341 tggccgtgtg ggcggtcgtc gagctcatgg gagcgcgtga gcacgagcgc ctcgtcatcc
     2401 agcacctgtt cgactggatc tcggtcggcc ggttctcggt cggcgcggac ctgcggctcg
     2461 acgtgctctc ggcaacgatg atcctggtcg tcaccggcat cgggttgctg atccacgtct
     2521 atgcgatcgg ctatatggag ggcgacccgc ggtacggcag gttcttcgcc tacctgaacc
     2581 tgttcgtgtt cttcatgctc ctgctggtct tggcggacaa ctacctcctt ctctacgtgg
     2641 gctgggaagg agtcggcctc tgctcgtacc tcctgatcgg cttctggttc gagaagaccg
     2701 agaacgcggt cgcggcgaag aaggcgttcg tcacgacgcg gatcggcgac accctcatgc
     2761 tgatcggcct cgcgctgatc gtggcgaagt tcggctcgct cgatttcacc gtcgtcctcg
     2821 gcacgcctgg ggagacgatc gcgaagggga cggcgacggc gatctcgctg ctcctgttcg
     2881 ccggggcgat cggcaagtct gcccaggtcc cgctccacgt ctggctgccg gacgcgatgg
     2941 ccggccccac gccggtctcg gccctgatcc acgcggcgac gatggtgacg gccggcgtct
     3001 acctcgtcgt gcggagcgcg ccgctcttcg agctctccgg gaccgccctg accgtcgtgt
     3061 tgatcgtcgg gctcgtcacg atgctgttcg ccgcgacgtg cgccctcgcg caggacgaca
     3121 tcaaacgggt cctcgcgtac tccacgatca gccagctcgg gttcatgttc atggcggcgg
     3181 gcatgcgctt ctacacgggg gcgatgttca tgctcgtcgc ccatgcgtcc tacaaggcgc
     3241 tgatgttcct cggtgcgggc tccgtgatgc atgggatgcg cgaagagacc gacctgcaac
     3301 ggatgggcgg gctgatccgg cggatgcccg tgacggggtg gacgttcgtc gtcggcgcgt
     3361 tcgcccttgc cggagtcttc ccgctcgcgg ggttcttcgc gaaggaccag atcctcgagt
     3421 tcgcgaaccg cacgggcagg acgtggatct acctcctcgg gacgctcggc gcgctcatgt
     3481 ccgctctgta catcgggcgg ctgctgttcc tcgccttctt cgggagaccg aggagcgagg
     3541 aggcagagca cgcgcacgaa tcgccggcgg tcatgacggt cccactggcg atcctcgcgg
     3601 tgggcgcgat cgtcgccggg ctcctgctgt cgacgtcggc ggaaggcacg ctcgcgaggg
     3661 tcctggagcc ggtggtcgga tcgctcccg
//
LOCUS       DASUVE010000059         3504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_970355, whole genome shotgun sequence.
ACCESSION   DASUVE010000059 DASUVE010000000
VERSION     DASUVE010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970355"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VF036_01230"
     CDS             <1..399
                     /locus_tag="VF036_01230"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6263883.1"
                     /translation="FIARMIFGGVVERFPTLKVCVMHGGGYLPFYPARFDHAYEARDD
                     CREHISRPPSTYLAQMYFDTMVFDPVMLSTLVQRWGADHVLLGTDYPFDMGEDDPLDL
                     LGRVVGLGDAERSMISGGNAVRLLGLDRSP"
     gene            complement(436..1374)
                     /locus_tag="VF036_01235"
     CDS             complement(436..1374)
                     /locus_tag="VF036_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6263884.1"
                     /translation="MSIARILVVDPLLISEGFRIGADWAGPEHEVLIPEGFDLEHLRS
                     LLPEAAALLTAHAPVTEEMIALAPALRLVAKPGAGVDNIDVAAAARRGVTVTNVPGAR
                     GRAVAEHALFLLLHLSRRGWLRDDPAWRDTLATQLGGKSLGIVGLGEIGAHVARFGEG
                     LGMQVIAHTRTPDPDRAPGVAVRFVDLDTLLREADAVILCAPLTDATRNMIDRRALRL
                     MKEDAYLINVARGPLVSTEDLFEVMRAGHLAGAGLDVTDPEPLPEDHGLHSLPHVLIS
                     PHNAGRTIESQTEALARMRENVRLVLSGSPPIDPVR"
     gene            1476..1784
                     /locus_tag="VF036_01240"
     CDS             1476..1784
                     /locus_tag="VF036_01240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX6263885.1"
                     /translation="MADNAVSVFASFRPREGRAGDLRSLLAWMVERTRAEPGCERYDL
                     YEERDAGGALHLFERYRNQQALEAHRATEHYIEYRRQVADLLQEPIGVVVLDPIDVDG
                     "
     gene            complement(1802..2716)
                     /locus_tag="VF036_01245"
     CDS             complement(1802..2716)
                     /locus_tag="VF036_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6263886.1"
                     /translation="MAIEGCELERITGEPEDLFARALRNEEFDVTELSLSSYLIVAGR
                     SSSPYLAVPAFPSRAFRHSAVYVRTDRGIDRPEDLVGMAVGVPEFQQTAALWIRGILA
                     DRHGVPPTAIRWRSGGLEHPGAEERIPVQVREGIDLQPIDGTSTLSAMLAEGRLDGLV
                     SPRPPSCFEEGDPVVRRLWPDHRAEERRFYRDTGLFPIMHVVAIRRSLADRYPWLAAN
                     VFRGFAGAKRLAIHDLEQTNFLRVTLPWIELDTVRALMGDDYWPYGLAANRPELESAI
                     RWSVEEGLSPRPLDPSELFAAGSLELEK"
     gene            complement(2847..3359)
                     /locus_tag="VF036_01250"
     CDS             complement(2847..3359)
                     /locus_tag="VF036_01250"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6263887.1"
                     /translation="MARVDLLDDERDLTPEQRTVADRIAESRGEVSRPFQLLLHAPAM
                     AERVAELGHLVRSGSSVADADRELVTLATGRAIGCSFVWESHLHAATVAGISEETIAM
                     LQRDPDGLGERERLLVSFVDELCRTRTVSEATFVAVHRLLGVPAVVELALTVGYYTML
                     GSVMGACDAC"
BASE COUNT          558 a         1182 c         1235 g          529 t
ORIGIN      
        1 ttcatcgcgc ggatgatctt cggcggcgtc gtggagcggt tccccaccct gaaggtctgt
       61 gtgatgcacg gcggcgggta cctgccgttc tacccggcgc gcttcgacca cgcgtacgaa
      121 gcgcgtgacg actgtcgcga acacatcagc cgcccgccga gcacctacct cgcccagatg
      181 tatttcgaca ccatggtgtt cgaccccgtg atgctgtcga cgctcgtgca gcgatggggt
      241 gccgatcacg tcttgctggg caccgactat ccgttcgaca tgggggagga cgaccccttg
      301 gacttgctcg gccgggtcgt gggtctcgga gatgcagagc gctcgatgat ctcggggggg
      361 aacgctgtgc gactcctggg gctggatcgc tcgccgtaac ggaggggagg gcgacggccg
      421 atccgaccgc cgcgatcagc gcaccgggtc gatcggagga gagccggaga gaacgagtcg
      481 cacgttctcc cgcatcctcg ccaacgcttc ggtctgggac tcgatcgtcc gtcccgcgtt
      541 gtgcggagag atcaggacat ggggcaggga atggagcccg tggtcctccg ggagaggctc
      601 cgggtcggtc acgtccaggc ccgcgccggc gagatggccg gcgcgcatca cctcgaagag
      661 gtcctccgtc gatacgagcg ggccgcgagc cacgttgatc aggtaggcgt cctccttcat
      721 caacctgagc gccctccggt cgatcatgtt ccgcgtggca tcggtcagcg gggcgcagag
      781 gatgacggcg tcggcctcgc gcagcagcgt atcgagatcc acgaaacgca ccgcgacgcc
      841 cggcgctcgg tcggggtcgg gagtccgcgt gtgggcgatg acctgcatcc cgagaccttc
      901 gccgaatcga gcgacgtgag cgccgatctc gccgagtccg acgatcccga gggacttccc
      961 gccgagctgc gtcgctagtg tgtcgcgcca agcggggtca tcgcgcagcc acccacgtcg
     1021 cgaaaggtgg agcaggagga acagggcatg ttcggcgacc gcccggcccc gcgcgccggg
     1081 gacgttcgtg accgtcacgc cgcgccgggc ggctgcggcg acgtcgatgt tgtccacgcc
     1141 ggcgcccggc ttcgccacga gccggagtgc cggagccagt gcgatcatct cctccgtcac
     1201 gggagcgtgg gccgtgagaa gggctgcggc ctccgggagc agtgagcgaa gatgctcgag
     1261 atcgaacccc tccgggatca gcacctcgtg ctccgggccg gcccagtcgg caccgatccg
     1321 gaagccctcg ctgatcagga ggggatcgac gacgaggatc cgggcgatgc tcaccagctc
     1381 ctctcgacgg acggccgata cactacgcgc gcgtcctcgg ggcgggccgc caccgcaccg
     1441 gatcgaggat cgctcgggtc cgttacgagg tgagcatggc cgacaacgcg gtttccgtct
     1501 tcgcttcgtt ccgtcctcgc gaagggcgag cgggcgacct ccgctcgctg ctcgcgtgga
     1561 tggtggaacg cacgagggcg gagccgggat gcgagcggta cgacctctac gaggagcgcg
     1621 atgccggcgg cgcgctccat ctgttcgagc gctaccggaa ccaacaggcg ctcgaggcgc
     1681 atcgagcgac ggagcactac atcgagtatc ggcggcaggt tgccgacctc ttgcaggagc
     1741 cgatcggcgt ggtcgtcctc gacccgatcg acgtggacgg ctgagggccg acaggccacg
     1801 ctcacttctc cagctccagc gacccggcgg cgaagagctc ggacggatcg aggggccggg
     1861 gcgagaggcc ttcctcgacc gaccaccgga tcgcggactc cagttccggc cggttcgccg
     1921 ccaaaccgta gggccagtag tcgtcgccca tcagcgcgcg cacggtatcg agctcgatcc
     1981 agggcagggt cacgcggagg aagttcgtct gttccaggtc gtggatcgcg agtcgcttcg
     2041 cccccgcgaa cccgcggaag acgttcgcgg cgagccaggg ataccgatcg gcgagcgacc
     2101 gccggatcgc gacgacgtgc atgatcggga acagtcccgt gtcccggtag aagcgtcgtt
     2161 cctccgcccg atgatcgggc cagagccgtc tgacgaccgg atccccctcc tcgaagcagg
     2221 acggcggtcg cggggacacg aggccgtcga gccgcccctc ggccagcatc gccgagagcg
     2281 tggaggtccc gtcgatcggc tggaggtcga tcccctcacg gacctggacg gggatccgtt
     2341 cctcggcgcc cgggtgttcg agcccgccgc tccgccacct gatcgcggtc gggggcaccc
     2401 cgtgacgatc ggcgaggatc ccgcggatcc acagcgcagc ggtctgctgg aactcgggga
     2461 ccccgaccgc catgccgacg aggtcctccg gacggtcgat gccgcggtcg gtcctgacgt
     2521 agacggcgga gtgtcggaac gcccgtgacg ggaacgcggg gacggcgagg taggggctgg
     2581 aactccttcc ggcgacgatg aggtagctgc tgagcgagag ctccgtgacg tcgaactcct
     2641 cgttccggag cgctctcgcg aagaggtcct cgggttcgcc ggtgatccgc tcgagctcgc
     2701 atccctcgat cgccacgcgc ccctccagga gcggtcgggt ccgatcgctg accgtgcagg
     2761 cgaacgagat ccgcagcggt ctcatccggt gggtgccgga ggcaagggat cgaggatagg
     2821 gaagggcgca tcgtgtccac gtgccctcaa cacgcgtcac acgcacccat gacggagccg
     2881 agcatcgtgt agtagccgac cgtgagcgcg agctcgacca ccgcggggac cccgaggagc
     2941 cggtgcacgg caacgaaggt cgcctcggac acggttcggg tcctacagag ctcgtcgacg
     3001 aacgacacga ggagcctctc gcgctcaccg aggccatcgg ggtctcgctg caacatcgcg
     3061 atcgtctcct cggagatccc cgcaacggtc gccgcgtgca ggtgcgattc ccagacgaac
     3121 gagcagccga tggctcgacc ggtggcgagg gtgaccagct cgcggtcggc gtcggccacg
     3181 ctcgatccgg agcgaacgag gtggcccagc tcggcgacgc gctcggccat cgcgggggcg
     3241 tggaggagga gctggaacgg gcgggaaacc tcgccacgac tctccgcgat ccggtcggcc
     3301 acggtccgct gctccggcgt gagatcgcgt tcatcgtcga gcaggtccac acgcgccatc
     3361 gctcacctcc cgttcctcct cggttcgccc ggttcttgtc ctctggaggt tcatctcacg
     3421 gggttcccga gggtgccgat cccgtccacc gagacctcca ctcgatcacc gggcaccggg
     3481 aacctgggcg gctcgaggcc gatc
//
LOCUS       DASUVE010000060         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1347693, whole genome shotgun sequence.
ACCESSION   DASUVE010000060 DASUVE010000000
VERSION     DASUVE010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347693"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VF036_01255"
     CDS             <1..606
                     /locus_tag="VF036_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263888.1"
                     /translation="SDPTIVRVFGANSHGKCCGPLVFEQDDGAHPSGINHYEWNGRTQ
                     ESGGSLLPKGDYFVKIRAEDASSLVGVSKPFKVAIARTYRAVKTKTKPARDYHHRSAA
                     TPIVIGGDCLVGVDAGNLRILCQGARVSVFWRWGLGPSQRIERANFVLDTQAGSCPKS
                     IRRSGHTKHESSFTVNEDLAGASGDCRIVTAKITFSYLQAS"
     gene            649..1719
                     /locus_tag="VF036_01260"
     CDS             649..1719
                     /locus_tag="VF036_01260"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX6263889.1"
                     /translation="MTTPTLERIREAPKVLLHDHLDGGVRPGTIVELADETGYRGLPT
                     RDVEELEAWMMRGADRKDLVLYLETFAHTVGVMQTTESLARVARECAEDLAADGIVYA
                     EVRYAPEQHLERGLTLDEVVETVQHGFTEGSQATGIRIGTLLTAMRTAARSLEIAELA
                     VRWRDRGVVGFDIAGAEAGYPPTRHLDAFDHVRRANFHLTIHAGEAFGLPSIWEALQL
                     CGAERLGHGVRIVDDIEVDPDGEARLGRVADLVRDRRVPLELCPTSNVHSGAAASIGE
                     HPIGLLMDRKFRVTVNTDNRLMSATTLSNEFAQLVDAFGIGWDRIRRLTINAMKSAFL
                     PFDERVAFLDDVIRPAFLEHDL"
     gene            1716..>2092
                     /locus_tag="VF036_01265"
     CDS             1716..>2092
                     /locus_tag="VF036_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX6263890.1"
                     /translation="MTAERDAAKDTPAIRDVAASGVSFRVVRTERPGSAEESAVLQGI
                     ELGQLIRSIVVRRGAGDYVFVLVPGGRQIAWARLRAHLGVSRLSLPDQNEARDATGYE
                     RGTITPLASSTGWPVVADATLADR"
BASE COUNT          343 a          697 c          732 g          320 t
ORIGIN      
        1 tccgatccga cgatcgtgcg ggtcttcggc gcgaactcgc acgggaagtg ctgcggccct
       61 ctcgtcttcg agcaggacga tggagcgcac ccgtcgggca tcaaccacta cgagtggaac
      121 ggcaggaccc aggagtcggg aggatcgctc ctgccgaaag gcgactactt cgtcaagatc
      181 cgagccgagg atgcatcgag cttggtgggc gtctcgaagc ccttcaaggt cgcgatcgcc
      241 cggacgtatc gggcggtgaa gacgaaaacg aagcccgcga gggactacca ccatcggagt
      301 gccgcgaccc ccatcgtgat cgggggggat tgcctcgtcg gcgtggatgc cggcaacctg
      361 cggatcctct gtcaaggagc gcgcgtgtcc gtgttctggc gatggggcct cggcccatcg
      421 caacggatcg agcgggccaa cttcgtgttg gatacacaag cggggagctg tccgaagtcc
      481 atccggcggt cggggcatac gaagcatgaa tcctcgttca ccgtgaacga agatctggcc
      541 ggagcgtcgg gtgattgccg aatcgtcacg gcgaagatca cgttcagcta cctccaagcc
      601 tcctgacccg ccgggttccc ggagcgatca gcacctacca tcaggcgcgt gaccacgccg
      661 acgctcgagc gcatcaggga ggcgccgaag gtcctcctcc acgaccatct cgacggcggc
      721 gtgcggccgg gcacgatcgt ggagctggcg gacgagaccg gctaccgggg cctcccgacc
      781 cgcgacgtgg aagagctcga ggcctggatg atgcggggag ccgatcgcaa ggacctggtc
      841 ctgtacctcg agacattcgc ccacaccgtc ggggtgatgc agacgaccga gtcgctggcg
      901 cgcgtcgccc gggagtgtgc ggaggacctc gccgccgacg gcatcgtcta cgcggaggtc
      961 cgctacgccc cggagcaaca cctcgaacga ggtctcacgt tggacgaggt cgtcgagacg
     1021 gtgcagcacg gattcaccga gggctcccag gcaaccggga tccggatcgg cacgttgctc
     1081 acggcgatgc gcacggccgc gcgatccctc gagatcgcgg agctcgccgt ccgctggcgg
     1141 gatcgcgggg tggtgggctt cgacatcgcg ggcgcggagg ccggatatcc gccgacccgc
     1201 cacctcgacg cgttcgacca cgttcgccgc gcgaacttcc atctcacgat ccacgcgggg
     1261 gaggcgttcg ggctgccgtc gatctgggaa gccctgcaac tgtgcggcgc cgagcggctc
     1321 gggcacgggg ttcgcatcgt cgacgacatc gaggtcgatc cggatggcga ggcccggctc
     1381 ggacgcgtcg ccgatctcgt ccgtgaccgg cgggtgccgc tcgagctgtg cccgacctcc
     1441 aacgtccact ccggagcggc ggcctcgatc ggtgagcacc cgatcgggct cctgatggat
     1501 cggaagttcc gcgtcaccgt gaataccgac aaccggctga tgtcggcgac gacgttgtcg
     1561 aacgagttcg cgcagctcgt cgacgcgttc ggcatcggat gggaccggat ccgccgtctc
     1621 acgatcaacg cgatgaagtc ggcgttcctc ccgttcgacg agcgcgtcgc cttcctcgac
     1681 gatgtgatcc ggccggcgtt cctcgaacac gatctatgac ggctgaacgg gacgcagcga
     1741 aggacacgcc ggcgatccgc gacgtggccg catcgggcgt ctccttccgc gtggtccgga
     1801 cggagcggcc gggcagcgcc gaggagagcg ccgtgctcca gggcatcgag ctcggacagc
     1861 tgatccggtc gatcgtcgtg cgccgcggcg caggcgacta cgtcttcgtc ctcgtgcccg
     1921 gcgggcgtca gatcgcctgg gcgaggctgc gcgcgcacct cggagtgagc cggctgtcgt
     1981 tgccggacca gaacgaggcc cgcgacgcca ccggctacga gcgggggacg atcaccccgc
     2041 tcgcttcgtc gacgggctgg ccggtcgtgg ccgacgcgac cctcgccgat cg
//
LOCUS       DASUVE010000061         3927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_218613, whole genome shotgun sequence.
ACCESSION   DASUVE010000061 DASUVE010000000
VERSION     DASUVE010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218613"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VF036_01270"
     CDS             <1..312
                     /locus_tag="VF036_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX6263891.1"
                     /translation="GGLDVVVRRNAFGSQNQSFETDLDVPVLGDPPVHAVFIRGPVVE
                     SVGAKATPLASLSDGRVVAVEQGNLVGTSFHPEINGEYRFHAYFLGKVRDRIAAQQAS
                     A"
     gene            451..2556
                     /locus_tag="VF036_01275"
     CDS             451..2556
                     /locus_tag="VF036_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX6263892.1"
                     /translation="MNVAIRPRGGGDEYADLEISLHRRDAVSWSVELRFSLPGSDGES
                     RLEADGPMLAEIDPRRLDLTGVDGEYGLELGRGLFAHGVGEAFRTAVATSQSVGATLR
                     VRLVMGSSAVALHGLRWETLRDPVGGATLLTDENLLFSRYLSSRDWRFVGVRAREDLT
                     ALALVAGPTDLGSIDANRPLAPIDVDAELARARDGLSSMELTTLPGVAAPTAENLLRH
                     VRRGCDVLYLVCHGYVVNDEPVLLLVDEDGRADPLLGSDLISRLAQLSRLPRLVVLAS
                     CQSAGAGAERTSEDAGVLAALGPRLAEIGVPAVVAMQGNISMATAATFTKEFFISLEE
                     DGLVDRAMAVARGTVRDRPDWWVPTLFMRLKSGRLWYVPGIAPGGEAFDKWPSLITSL
                     SNGVCTPIIGPGISDALLGSRQEIAREWARSYRFPMAEHFSDSLPQVAQYLSIHQDRP
                     FLRAELEKHLRRTLAERYQDELPMDVLRGEASLEEVIAAAWEARHAREEVDPYSVLAQ
                     LPVPVYITTQPSQLLVRALTEAGREPQVDVCRWNGDEDWSESVFDRHPDYRPTPERPL
                     IFHLLGTLDDPESLVLTEDDYFDFLIGVARNQDLVPSAVRRRLSDSALMFVGFRLDEW
                     DFRVLYRSLMNSEGSRRRRGYTHVAVQIDPEESTTIDASGARNYLEEYFQDARVSVYW
                     GTTEHFVKELHERWEGRTP"
     gene            2553..>3927
                     /locus_tag="VF036_01280"
     CDS             2553..>3927
                     /locus_tag="VF036_01280"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263893.1"
                     /translation="MNGAAPTRSDDPYVGPVSFRAGDLLYGRDREREELLDLLIAERI
                     VLLYSPSGAGKTSLIEAALVPALVTADFEVLPTIRVTHALPPEPGLPVPRNRYVMGVL
                     LSLEERVPPERQLPMDELAAITLQEYLEAHVDRDGRPGNEVLVFDQFEEVLTADPTDD
                     AAKHAFFQELGHALRNPELWALFSMREDFLAALDPYLKYVPTRLRSTFRLDLLSVPAA
                     LEAIRRPAQRAGVHFTEEAAEQLVDDLRVVRVQRAGGVADVLGSFVEPVQLQVACHLL
                     WSMLPEDATKITRADVDALGRVDHALGEYYAERVQAIAVQTGVPERLIRDWFEERLIT
                     PQGLRSQVLEGPSSENGHAVLAELLDAHLVRAETRRQATWYELAHDRLIDPVRHDNAA
                     WRTEHLSPLERSAMHWEQEGKPDRLLLLGAELAAAESGVAVTSGSLSTLQREFLDASR
                     KADAQANR"
BASE COUNT          599 a         1335 c         1353 g          640 t
ORIGIN      
        1 ggcgggctcg acgtcgtcgt gcgccgtaac gcgttcggct cgcagaacca gtcgttcgag
       61 accgacctcg acgtgccggt gctcggcgac cctccggtgc acgcggtgtt catccgcggc
      121 cccgtggtcg aatcggtcgg agcgaaggcc acgccgctcg cctcgctctc cgacggacgc
      181 gtcgtcgcag tcgagcaggg caacctggtc ggcacgagct tccaccccga gatcaacggg
      241 gagtaccgct tccacgcgta cttcctcggc aaggtccgcg accgtatcgc ggcgcagcag
      301 gcgagcgcgt agcggcgatc tgttcggctc ccgcctcagg gagagccctg ttgcgctcgt
      361 acgctcaggg gtcttctctg gactcagagg gggtcgcggt gcggcggctc ggctcgacgg
      421 gggatgcagc atgaccgagc aggtgtgcgc atgaacgtgg cgatcagacc gcgtggcggg
      481 ggcgacgagt acgccgatct cgagatcagc ctccaccggc gtgacgcggt ttcgtggagc
      541 gtcgagctcc ggttctccct ccccggctcc gacggcgaga gccggctcga agccgatgga
      601 ccgatgctcg ccgagatcga tccgcgccga ctggatctca ccggcgtcga cggggagtac
      661 ggcctcgagc tcggtcgtgg cctgttcgcc cacggcgtgg gtgaggcatt ccgcaccgcc
      721 gtggcgacct cccagagcgt tggagccacc ctgcgcgttc gcctcgtcat gggcagcagc
      781 gccgtcgccc tgcacggcct gcgctgggag acgctgcgcg atccggtggg cggggcgacg
      841 ctcctgaccg acgagaatct gctgttctcg cgctacctca gcagtcggga ctggcggttc
      901 gtcggcgtcc gcgcgcggga ggacctgact gcgctcgccc tcgtcgcggg accgacggat
      961 ctgggatcga tcgacgcgaa ccgcccgctg gcgccgatcg acgtcgatgc cgagcttgcg
     1021 cgggcgcggg acggcctcag ctcgatggaa ctgaccacgc tcccgggagt cgccgcgccg
     1081 accgcggaga acctgctccg tcacgtccgt cgtggctgcg acgtcctgta cctggtctgc
     1141 cacgggtacg tcgtcaatga cgaacccgtg ctcctgctcg tcgacgagga cggccgagcg
     1201 gatccgctcc tcggcagcga cctgatcagc cgactcgcgc agctgtcgcg gctaccgagg
     1261 ctggtcgtcc tcgcctcgtg ccagagcgcg ggcgccggcg ccgagcgaac gagcgaggac
     1321 gcgggtgtgc tcgccgcgct cggcccacgg ctggctgaga tcggagtccc tgccgtggtc
     1381 gcgatgcagg ggaacatctc gatggctacc gctgccacgt tcaccaagga gttcttcatc
     1441 agcctcgagg aggacggact cgtcgaccga gccatggcgg tcgctcgggg aaccgtgcga
     1501 gatcgccccg attggtgggt cccgacgctg ttcatgcgtc tgaagagcgg tcggctctgg
     1561 tacgtccccg gaatcgcccc cggcggtgag gcgttcgaca agtggccgtc cttgatcacc
     1621 tcgctgagca acggagtgtg cacgccgatc atcggcccgg ggatcagtga cgccctgctg
     1681 gggagcaggc aggagatcgc ccgtgaatgg gcgaggagct accgcttccc gatggcggaa
     1741 cacttctcgg acagcctccc gcaggtggcg cagtacctgt cgatccacca ggaccggcca
     1801 ttcctgcggg cggagctgga gaagcatctg cgccgaacgc tggccgagag gtaccaggac
     1861 gagctcccaa tggacgtgct ccgcggtgag gcgtcactcg aggaggtcat cgccgcggcg
     1921 tgggaggcgc gacacgcgcg cgaggaggtc gacccgtact ccgtgctggc gcagctcccg
     1981 gtccccgtct acatcacgac gcagccatcg caactcctgg tgcgcgcgct gaccgaagcg
     2041 ggtcgggaac cccaggtcga cgtgtgccgc tggaacggcg acgaggactg gtcggagtcc
     2101 gtgttcgatc ggcatcccga ttaccgcccg accccggagc gacccctgat cttccacctc
     2161 ctcggcacgc tcgacgaccc ggaatcgctg gtactgaccg aggacgacta cttcgacttc
     2221 ctcatcggcg tcgcccgcaa tcaggatctg gtgccgtcag ccgtacgccg caggttgtcg
     2281 gactccgccc tgatgttcgt gggattccgc ctggacgagt gggacttccg tgtgctctac
     2341 cgcagtctca tgaactccga gggcagcagg cggcgtcgcg ggtacaccca cgtcgccgtg
     2401 cagatcgatc ccgaggagag cacgaccatc gacgcgagcg gtgcccggaa ctacctcgag
     2461 gagtacttcc aggacgcccg cgtgtcggtc tactggggga ccaccgagca cttcgtcaag
     2521 gaactgcacg agaggtggga ggggcggacg ccgtgaacgg tgctgcaccc acccgctcgg
     2581 acgacccgta cgtcggtcca gtctcgttcc gcgccggcga cctgttgtac gggcgcgatc
     2641 gagagcgcga ggaactcctc gatctgctga tcgccgaacg gatcgtactg ctgtactcgc
     2701 cgtccggtgc cggcaagact tccctgatcg aggccgccct cgtgccggcg ctcgtgaccg
     2761 ccgacttcga ggtgctgccg acgattcgcg tcacccacgc ccttcctccc gagccgggac
     2821 tgcccgtgcc gcggaatcgc tatgtgatgg gcgtgctgtt gtcgctcgag gagcgggtgc
     2881 ctccggagcg tcagctgccg atggatgaac tggccgccat cacgttgcag gaatacctcg
     2941 aagcccatgt cgaccgggat ggccgcccgg gcaatgaggt cctcgtgttc gaccagttcg
     3001 aggaggtgct gaccgcggac ccgaccgatg acgcggccaa gcacgccttc ttccaggaac
     3061 tcggtcacgc gctgcggaac cccgagctct gggcgctctt ctcgatgcgg gaggacttcc
     3121 tcgctgcgtt ggatccgtac ctcaagtacg ttccgacgcg gttgcgctcc accttccgac
     3181 tcgacctcct ttcggtgccg gcagcgctcg aggccatccg acgtcctgct cagcgggccg
     3241 gggtccattt caccgaggaa gccgcagagc agctggtgga tgacctgcgc gtcgtacggg
     3301 tgcagcgggc ggggggcgtc gccgacgtgc tcggctcctt cgtcgagccg gtccagctgc
     3361 aggtcgcctg tcacctcctc tggtcgatgc ttcctgagga tgcgacgaag atcacccgag
     3421 cggacgtcga cgcgctcggc agggtggacc atgcgctggg cgagtactac gccgagcggg
     3481 tgcaggcgat cgcggttcag accggcgttc cggagcgtct catccgcgat tggttcgaag
     3541 aacgactgat caccccgcag ggcttgcgca gccaggtgct cgagggccca tcgtccgaga
     3601 acgggcacgc ggtgctggcc gaactgctcg acgcccacct ggtgcgggcg gagacccgcc
     3661 gacaagccac ctggtacgag ctggcacatg accgattgat cgatccggta cgacacgaca
     3721 acgccgcctg gcgcaccgag cacctcagcc ccctggagcg ctcggccatg cattgggagc
     3781 aggagggaaa gcccgaccgg ctgctcctgc tcggcgccga gctcgccgct gccgagagtg
     3841 gggtcgcggt gacgagcggt tcgctgtcga cgctccagcg cgagttcctc gacgcgtccc
     3901 gaaaggcgga cgcgcaggcg aatcgcg
//
LOCUS       DASUVE010000062         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_407212, whole genome shotgun sequence.
ACCESSION   DASUVE010000062 DASUVE010000000
VERSION     DASUVE010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407212"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VF036_01285"
     CDS             complement(<1..329)
                     /locus_tag="VF036_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263894.1"
                     /translation="MAIAGEDLHAMRRARVGVTAAFAAHAVLSGLLGPWVPRIKAQAG
                     LDASGLGIALTGFAIGLLAGTRVADPAIRRWGGRRVVRGGIPMMGVGFALLPWAGTLP
                     SLTLLFFG"
     gene            394..2001
                     /locus_tag="VF036_01290"
     CDS             394..2001
                     /locus_tag="VF036_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6263895.1"
                     /translation="MPASAPDAGASPWWRAGVLYQVYPRSFADSTGNGHGDLRGVIAH
                     LDHLSWLGVDGIWLSPINPSPDADWGYDVADYTDVHPDFGTLEDLDRLVAEAADRGIR
                     VLIDLVPNHTSDRHPWFVEARSSRSSERRDWYVWADGGPDGTPPNNWRSSFGGPAWTW
                     DEATGQYYLHNFLPEQPDLNWWNRGVADAFDDIVRFWFDRGISGFRIDVAHALVKDRS
                     LRDDTPALDTDHPSIRRHGLRSDFSMNRPEAHDVLRRWRTIADRYDPRRVLLGETWVL
                     DLESLARFYGSGEDELHLALNVPFVFSTPGAEMREIVERTEGTLPTGAWPLWNGSNHD
                     AGRFMTRWCDGDERRARAALVILLTLRGTPLLYYGDEIGMPNVDVPRDRLMDPVGRRH
                     WPEDPGRDKGRTPMQWTREGGFSDGGVEPWLPMGDARSRNVADQRDDPASFLSFCRDL
                     IGVRRDREDLRSGSYEPLGSPEGVWAWRRGSGTMVIVNHGDEAMTLPTQSGTVLIDTR
                     RQRTGEQVEGPLRLDPWEALILGEGEA"
     gene            complement(1998..>2312)
                     /locus_tag="VF036_01295"
     CDS             complement(1998..>2312)
                     /locus_tag="VF036_01295"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263896.1"
                     /translation="AIVDVRAAYGEDVEHMRSILEELFNELERDRTLSEWIHEAPSVL
                     GVERMGQDALVLRVVADTRPSKVMDVERLLRERISRRLAEKGIKIPVIPVAATGPGPD
                     GR"
BASE COUNT          351 a          823 c          803 g          335 t
ORIGIN      
        1 ccgaagaaga gcagcgtgag cgacgggagg gtccccgccc acggcaggag cgcgaaccct
       61 acgcccatca tcgggatacc gccgcggacg acccgtctgc cgccccaacg ccggatcgcc
      121 gggtccgcca ctcgcgtccc cgccaacagg ccgatcgcga accccgtcag ggcgatcccg
      181 agtcccgagg catccaagcc cgcctgggcc ttgatgcgtg ggacccacgg accgaggagg
      241 ccggacagga ccgcgtgtgc cgcgaacgcg gcggtgaccc ccacccgtgc gcggcgcatc
      301 gcgtgcaggt cctccccggc gatcgccatc gagagagtct ggtcgcgcgg ccgcgaaccg
      361 gcacatccgg gaccgttcgc tagtctgccc cccatgcccg cgtccgctcc cgacgccggc
      421 gcgtcgccgt ggtggcgcgc cggcgtcctg taccaggtct atccacgctc cttcgccgac
      481 tccaccggga acggtcacgg cgacctccga ggggtcatcg cgcatctcga ccacctgtcg
      541 tggctggggg tcgacggcat ctggttgagc ccgatcaatc cttcgcccga cgcggactgg
      601 ggatacgacg tggcggacta caccgacgtc caccccgact tcggcacgct cgaggatctc
      661 gatcgattgg tggcggaagc tgccgatcgg gggatccgcg tgctgatcga tctcgtgccg
      721 aaccacacga gcgaccggca tccctggttc gtcgaggcgc ggtccagccg ttcgtccgag
      781 cgacgcgact ggtacgtgtg ggccgacggg gggccggacg gcacaccgcc gaacaactgg
      841 cgatcgtcct tcggcggtcc cgcgtggacc tgggacgagg cgaccgggca gtactacctc
      901 cacaacttcc tgccggagca gccggacctg aactggtgga accgcggcgt cgccgacgcg
      961 ttcgacgaca tcgtgcggtt ctggttcgac cgggggatct cggggttccg gatcgacgtc
     1021 gcccatgccc tcgtgaagga tcgatccctc cgggacgaca cgccggccct cgatacggac
     1081 caccccagca tccgccgcca cggtctgcgc tcggatttca gcatgaaccg gcccgaggcg
     1141 cacgacgtgc tccggcggtg gcggacgatc gccgatcggt acgacccgcg acgggtgctg
     1201 ctcggcgaga cctgggtgct cgacctcgag tcgctggcgc gcttctacgg gagcggggag
     1261 gacgagctgc acctcgccct gaacgtgccg ttcgtcttct cgacccccgg agcggagatg
     1321 cgggagatcg tcgaacggac cgaggggacg ctccccaccg gcgcgtggcc gctctggaac
     1381 ggctccaacc acgacgccgg ccggttcatg acgaggtggt gcgacggaga cgagcgtcgg
     1441 gcgcgagcgg cgctcgtgat cctcctcacg ttgcgcggca ccccgttgct gtactacgga
     1501 gacgagatcg gcatgccgaa cgtcgacgtc cctcgcgacc gactgatgga ccccgtcggc
     1561 cggcgccact ggccggagga cccgggccgg gacaaaggga ggacgccgat gcagtggacg
     1621 cgcgagggag gtttctcgga cggcggcgtc gagccgtggc tcccgatggg cgacgctcgc
     1681 tctcgcaacg tcgccgatca acgcgacgac ccggcctcgt tcctctcgtt ctgccgggac
     1741 ctgatcggcg tgcgacgaga tcgagaggac ctccgttcag gttcgtacga gccgctcgga
     1801 tctcccgagg gcgtctgggc gtggcggcgt ggctccggga cgatggtgat cgtgaaccac
     1861 ggggacgagg cgatgacgct cccgacccag tcgggcacgg tcctgatcga caccaggagg
     1921 cagcggacag gagaacaggt cgagggcccg ctccggctcg atccctggga ggcgctgatc
     1981 ctcggtgagg gagaggccta gcgcccgtcc ggtccggggc cggtcgcggc gaccgggatc
     2041 accgggatct tgatcccctt ctccgcgagc cggcggctga tgcgttcgcg gaggagccgt
     2101 tccacgtcca tcaccttcga gggccgcgtg tcggcgacca cccggagcac gagcgcgtcc
     2161 tgccccatcc gctcgacccc cagcacgctc ggagcctcgt ggatccactc ggacagggtc
     2221 cgatcgcgct cgagctcgtt gaagagctcc tcgaggatgc tccgcatgtg ctcgacgtcc
     2281 tcgccgtagg cggcccggac gtccacgatc gc
//
LOCUS       DASUVE010000063         4629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_219916, whole genome shotgun sequence.
ACCESSION   DASUVE010000063 DASUVE010000000
VERSION     DASUVE010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219916"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VF036_01300"
     CDS             complement(<1..561)
                     /locus_tag="VF036_01300"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX6263897.1"
                     /translation="MKILPRRRRGTASSTDVERDAIARLVRAPLFELIPLRDALERAD
                     ALPAGAATTVTASPSHGIEATVELCEALIARGHEATPHLAAHMFRDRPHVAELLDRCR
                     SAGIRSAFVVGGDAKDRGEIHDGLALIRLMEDLGHPFTSVGVPGYPEGHPSIPGDVLI
                     ASLRAKQAHADHVTTQMGFDPDAIATW"
     gene            809..1663
                     /locus_tag="VF036_01305"
     CDS             809..1663
                     /locus_tag="VF036_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263898.1"
                     /translation="MIVRTGRIVFSGDDGEIHVLSLSTGEDRTVTSIRGPQFDPDGHG
                     PLIVFRDSRAGVNVNDDIAVIRTDGTGYRNLTRSPDANEWGPVWSPDGRRIAYSSDEN
                     GMPQLFVMDADGSNARQLSDVWGEYPNWSPDGSRIVFASSMGGTTPFGDPDYDVFVMD
                     ADGSDEVNLTHSPDINEGYPTWSPDGEWIAVQSTRGTPPSFEPPPHDPERQSDEDVWV
                     MRANGSDARNLTLDLVRQNKFPDWSDRGLLIDREGSIVILDPDTGAQDDVSERTGVLG
                     VFPAWIER"
     gene            complement(1660..2829)
                     /locus_tag="VF036_01310"
     CDS             complement(1660..2829)
                     /locus_tag="VF036_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX6263899.1"
                     /translation="MTVIEDPGILLYTRIRKSPYFHASRKHGVARYSVYNHTYHPRHY
                     GDPVAEYWHLVNAVTLWDVGVEKQIQIKGPDAFDLTNMIVPRDLNKCAVDQCKYVFIT
                     APDGGILNDPVLLRVEEDEFWLSLADSDVNLYALGIAAANGMDVTVQEIDVAPVQIQG
                     PNAKKVLVELFGDAILDVPYYGLMKAELDGMRVVISRTGYTGEVGYEIYLRDASEHGV
                     ALWDRVLEAGKPHDLRVIGPCHIRRIEGGILAFGADMWYENNPFEVGYHYTWMVDLEQ
                     EADFMGKEALKRIKAEGVKQKLVGVDIEGEPLGTYIDNEMLDFFPASANGSVVGRVTS
                     ACYSPRLEKNIGLAMLPIEHTELGTALQVDTSKGTVGATVVPMPHWDPTKEIPKG"
     gene            complement(2866..>4629)
                     /locus_tag="VF036_01315"
     CDS             complement(2866..>4629)
                     /locus_tag="VF036_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX6263900.1"
                     /translation="SLPQLAADADEEIVHPILRHQDLSLYFRQRGDRYAVGNYRHEPI
                     LFEPDDLRPYREGEPQPSIEPFTPEHFEVAYEESIRLLPAVKDTLDETTAINGMFSFT
                     PDMGSIVGESADVRGFWICEAVWVTHAGGMGRMAAEWIAGGEPSMDLAEADANRFYPF
                     MTTAPYVRARGAQQYREVYDILHPLQQMSHPRELKLTPFYARHRDLGAEFFSGAGWER
                     PQWFEANHVLTGAPWESRTGWAAQNWSPCVGTEHIATRERAAMFDITPFAKFDVTGAD
                     ALSFLERISANRIDRPVGTIIYTSLLTSSGGIRCDLTITRKDEDRFRVVTGGGSGQHD
                     RAWLLAQRRPGERVHLTVRTGSLFALGLWGPKARDVLQTVTDVDVSNEAFPYMTARYL
                     NVGEVDPVWTQRISYAGELGWELYGQIAMGQRAWDVLWEAGQEHGVVAAGGGAFDSLR
                     LEKGYRLWGQDIHTEHDPFEAGLGFAVRMDKGGFQGREALQRKLDLGLTEKLVCITLD
                     DPAAVVMGKEPIRHDGRVVSYVTSAAYGYSIGRGIVYGYLPVDLAVEGTPVEVEYFGD
                     RLAATVAAEPLWDPKGERLRA"
BASE COUNT          724 a         1613 c         1539 g          753 t
ORIGIN      
        1 ccaggtcgcg atggcatcgg ggtcgaagcc catctgcgtc gtcacgtggt ccgcgtgcgc
       61 ctgcttcgcc cggagggagg cgatgagcac gtcgcccggg atcgagggat gaccttccgg
      121 gtagccgggc acgccgaccg atgtgaacgg gtgacccaga tcctccatca gccggatcaa
      181 cgccaagccg tcgtggatct cccctcgatc cttcgcgtcg ccgccaacca cgaacgcgct
      241 ccggatcccc gcggatcggc atcggtcgag gagctcagcc acgtggggtc gatctcggaa
      301 catgtgcgcc gcgaggtgtg gcgtggcctc gtgccctcgg gcgatcaacg cctcgcagag
      361 ctcgacggtc gcctcgatcc cgtgactcgg cgaggcggtc accgtggtcg ctgcccccgc
      421 gggaagcgca tcggctcgct cgagcgcgtc gcggagcggg atcagctcga agagcggtgc
      481 tcgaacgagc cgagcgatcg catctcgctc gacgtccgtc gacgatgcgg ttccacggcg
      541 tctgcgcggc aggatcttca tggcgaacag ccatggtgac gcacccacga aggacgaggc
      601 aaagctccat cgcgacccca ggtcagctct tcgctcacgc gcccccggga acgcgagatc
      661 gcttcccggt gtagcacgcg gcatggatcc ctcgaggtgc cgcaggcgag ccaccctcat
      721 ccccatcgcc ctgctcgtgg cgtgcaccgg gtcctgcggg tacggaggct cgccatcgcc
      781 atccccggcg cagcccggca cggagtccat gatcgtgcgc acggggcgca tcgtgttcag
      841 cggcgatgat ggcgagatcc acgtcctctc gctctccacc ggcgaggacc ggacggtcac
      901 gtcgatccgc ggcccgcagt tcgatcccga cggccacggg ccgctcatcg tcttccggga
      961 ctcacgcgcc ggcgtgaacg tcaacgacga catcgccgtg atccgtaccg acgggacggg
     1021 ctaccggaac ctcacccggt cgccggatgc gaacgaatgg ggaccggtct ggtcccccga
     1081 cggccggcgg atcgcctact cgtccgacga gaacggcatg ccgcagctct tcgtgatgga
     1141 cgccgacggc tcgaacgcgc ggcagctctc ggacgtctgg ggcgagtacc cgaactggtc
     1201 gcccgacggc tcccggatcg tgttcgcgtc ctcgatggga gggacgacgc cgttcggtga
     1261 ccccgactac gacgtgttcg tgatggacgc cgacgggtcc gacgaggtca acctcacgca
     1321 ctcgccggac atcaacgagg gatacccgac ctggtcgccc gacggagagt ggatcgcggt
     1381 ccagtccacg cgaggcacac cgccgtcctt cgagcctccg ccccacgacc ccgagcggca
     1441 gagcgacgag gacgtgtggg tgatgcgcgc gaacgggtcc gatgcacgga acctgacgtt
     1501 ggacctcgtg cggcagaaca agttccccga ttggtcggac cggggccttc tgatcgaccg
     1561 tgagggatcg atcgtgatcc tcgacccgga caccggagcg caggacgacg tcagcgaacg
     1621 gaccggggtg ctcggggtgt tccccgcctg gatcgagcgc tagcccttcg ggatctcctt
     1681 cgtcgggtcc caatggggca tcgggacgac cgtcgcgccc accgtgccct tcgacgtgtc
     1741 gacctggagc gccgtgccca actcggtgtg ctcgatcggc agcatcgcga ggccgatgtt
     1801 cttctccagc cgaggggagt agcacgccga cgtgacccgc ccgaccacgc tcccgttcgc
     1861 ggatgccggg aagaagtcga gcatctcgtt gtcgatgtac gtcccgagcg gctcgccctc
     1921 gatgtcgacc ccgacgagct tctgcttgac gccctcggcc ttgatccgct tcagagcctc
     1981 cttgcccatg aagtcggcct cctgctcgag atcgaccatc cacgtgtagt ggtagccgac
     2041 ctcgaacggg ttgttctcgt accacatatc cgcgccgaag gccaagatcc ctccctcgat
     2101 gcggcggatg tggcacgggc cgatcacgcg gaggtcgtgc ggcttcccag cttcgagcac
     2161 ccggtcccac agggccaccc cgtgctccga cgcgtcgcgc aggtagatct cgtaccccac
     2221 ctcgccggtg tagccggtgc gggagatgac cacccgcatc ccgtcgagct ccgctttcat
     2281 cagcccgtag tacgggacgt cgaggatcgc gtcgccgaac agctcgacga ggaccttctt
     2341 ggcgttcggc ccctggatct ggaccggcgc gacgtcgatc tcctgcacgg tgacgtccat
     2401 gccgttggcg gcggcgatcc cgagcgcgta gaggttcacg tccgagtcgg cgagcgagag
     2461 ccagaactcg tcctcctcga cgcggaggag gacgggatcg ttcaagatcc ccccgtctgg
     2521 tgcggtgatg aacacgtact tgcactggtc cacggcgcac ttgttgaggt cgcgcggcac
     2581 gatcatgttc gtgagatcga aggcgtccgg gcccttgatc tggatctgct tctcgacgcc
     2641 gacgtcccag agggtcacgg cattcacgag gtgccagtac tccgccaccg gatcgccgta
     2701 gtggcgaggg tggtacgtgt ggttgtagac gctgtagcga gccacgccgt gcttgcgtga
     2761 ggcgtggaag tacggcgact tgcggatcct cgtgtagagc aggatccccg ggtcctcgat
     2821 cacggtcatc gttcggttcc cctcttctcg ctcggtcttc ctctctcagg cccgcaggcg
     2881 ctcgcccttg gggtcccaca acggctcggc ggcgacggtg gccgccagac ggtcgccgaa
     2941 gtattccacc tcgaccggcg tcccttccac ggcgagatcg accgggaggt agccgtacac
     3001 gatgccacgc ccgatcgagt acccgtacgc ggcgctcgtc acgtaggaga cgacccggcc
     3061 gtcgtgcctg atcggctcct tgcccatcac cacagcggcg ggatcgtcga gggtgatgca
     3121 gacgagcttc tccgtgaggc cgaggtcgag cttgcgctgc agcgcctccc gcccctggaa
     3181 cccgcccttg tccatcctga cggcgaaacc gagcccggcc tcgaacgggt cgtgctccgt
     3241 gtggatgtcc tggccccaga gccggtagcc cttctcgagg cggagagagt cgaaagcccc
     3301 gccgccggcc gccacgaccc cgtgctcctg ccccgcctcc cacaggacat cccaggcgcg
     3361 ctggcccatc gcgatctgcc cgtagagctc ccatccgagc tcaccggcgt aggagatccg
     3421 ctgcgtccag acgggatcga cctcgcccac gttcaggtac cgcgcggtca tgtatgggaa
     3481 cgcctcgttc gagacatcga cgtcggtgac ggtctgaagg acgtcacgcg ccttcggacc
     3541 ccagagtccg agcgcgaaga gcgacccggt gcgcacggtc aggtgcacac gctcgccggg
     3601 gcgtcgctgc gcgagcagcc acgcgcggtc gtgctggccg gacccgccgc ccgtgacgac
     3661 ccggaagcga tcctcgtcct tgcgggtgat cgtgaggtcg caccggatgc cgcccgacga
     3721 cgtcagaagc gaggtgtaga tgatcgtccc gacggggcgg tcgatccggt tggcgctgat
     3781 ccgctcgagg aacgagagcg catcggcgcc ggtgacgtcg aacttcgcga acggcgtgat
     3841 gtcgaacatc gccgcgcgct cgcgcgtggc gatgtgctcc gtccccacgc acggcgacca
     3901 gttctgggcc gcccacccgg ttcgggactc ccacggggcg ccggtcagca cgtggttcgc
     3961 ctcgaaccac tggggccgct cccagccggc gcccgagaag aactcggcgc ccaggtcccg
     4021 gtggcgggcg tagaacggcg tcagcttcag ctctcgcgga tgcgacatct gctggagggg
     4081 atgcaggatg tcgtagacct cgcggtactg ctgcgcgccg cgcgcccgca cgtacggcgc
     4141 cgtcgtcatg aacgggtaga agcggttcgc gtcggcctcg gcgagatcca tcgagggctc
     4201 accgcccgcg atccattcgg ccgccatccg gcccatgcca ccggcgtgcg tgacccacac
     4261 ggcctcgcag atccagaaac cgcggacgtc cgcggactct ccgacgatcg agcccatgtc
     4321 gggggtgaac gagaacatcc cgttgatcgc ggtcgtctcg tccagcgtgt ccttcaccgc
     4381 agggaggagc cggatgctct cctcgtaggc cacctcgaag tgctcgggcg tgaacggctc
     4441 gatgctcggc tgcggctcgc cttcccggta cggccggaga tcgtccggct cgaacaggat
     4501 cggctcgtgc cggtagttcc ccacggcgta ccggtccccg cgctgacgga agtacaggga
     4561 caggtcctgg tgtcgaagga tcggatggac gatctcctcg tcggcgtcgg cggcgagctg
     4621 agggagcga
//
LOCUS       DASUVE010000064         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_972947, whole genome shotgun sequence.
ACCESSION   DASUVE010000064 DASUVE010000000
VERSION     DASUVE010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972947"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VF036_01320"
     CDS             complement(<1..287)
                     /locus_tag="VF036_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HEX6263901.1"
                     /translation="MEPWSFEAKGRTDEHVVDSRALRGNALGDPHERPVWVSTPPGYD
                     DDTERRYPSVYVIQGLTGQLDAWRNRSPFRRNFPELADDLFARGEAPPVIVV"
     gene            345..896
                     /locus_tag="VF036_01325"
     CDS             345..896
                     /locus_tag="VF036_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6263902.1"
                     /translation="MRADADSDRLVTDRLVLRRSRPEDAETISAYRSDPDVNRTQGWE
                     RTDIESVRADIEEMAARTPGQPGGWVQFSVERREDGRLVGDVGISPADGEPGVLKVGY
                     TVSPDVQGHGYATEAVRALVSYAIDTLGAEVVRAYADADNASSIRVAEKVGMHLIERS
                     EHREGNEVWSIVRYERRRDAASG"
     gene            complement(917..1765)
                     /locus_tag="VF036_01330"
     CDS             complement(917..1765)
                     /locus_tag="VF036_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX6263903.1"
                     /translation="MAALAIATVSPPASAAPLCFARTGSIVGTPGPDVLRGTPGSDTI
                     LGLGGNDVIVGRGGNDRICGGDGADSLSGGRDRDSIEGGANGDTIDGGPGKDRLIAGA
                     GDDSVAGGTGRDRLVGMDGNDVLNGGRGRDSITYFLARRSVHADLARHRANGEGIDLL
                     LRIESVEGSPYDDVLLGSRLPNAIDGGNGSDVVDGRGGNDRLIGGRGDDRLHGRAGND
                     VIDDSLGVAQGVFPDTGRDHLDGGVGDDRLDAVDEVGGNDTLGGGPGTDVCLADVGDV
                     RWSCGP"
     gene            1902..>2288
                     /locus_tag="VF036_01335"
     CDS             1902..>2288
                     /locus_tag="VF036_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263904.1"
                     /translation="MRGSGAPIVSNGDIADLLWRASDEETTHRRRALRKASGAARFWS
                     EEAADLAEAGRPLTELRAVGPWVAERLQAWLDAPPDLPEPDATRIGFLTLAEVRAVLD
                     REPAWEGTPHADLQIHTTDSDGSLPLR"
BASE COUNT          309 a          844 c          796 g          339 t
ORIGIN      
        1 accacgatca ccgggggtgc ttcccctcgt gcgaagaggt cgtcggcgag ctccgggaag
       61 ttccgacgga acggtgaccg gttcctccac gcgtcaagct gcccggtgag gccttggatc
      121 acgtagacgg atgggtatcg ccgctcggtg tcgtcgtcgt aaccgggagg ggtggagacc
      181 cagacgggac gctcgtgggg atcgccgagg gcgttccctc ggagcgctcg gctgtccacg
      241 acgtgctcgt cggttcgccc cttcgcctcg aaggaccacg gttccatctg ctccctcctc
      301 tccttcctct ggccctcgga ggctactggg tacgctgtgc ggccatgcgg gccgacgccg
      361 actccgaccg cctcgtcacg gatcggctcg tcctccgccg ctcgcgaccc gaggacgccg
      421 agaccatctc cgcctaccgc agcgaccccg acgtgaaccg tacgcagggc tgggagcgga
      481 ccgatatcga gagcgtcagg gcggacatcg aggagatggc cgcccgcaca ccgggacagc
      541 cgggagggtg ggtgcagttc tcggtcgagc gccgcgagga cggccgactg gtcggcgacg
      601 tgggcatcag ccccgccgac ggcgagccgg gcgtcctcaa ggtcggctac acggtctcgc
      661 cggatgtgca gggtcacggg tatgcgaccg aggccgtccg ggcgctggtc tcgtacgcga
      721 tcgacaccct cggtgccgag gtcgttcgtg cctacgcgga cgcggacaac gcttcgtcga
      781 tccgcgtggc cgagaaggtc ggcatgcacc tgatcgagcg atccgagcat cgcgagggga
      841 acgaggtctg gtccatcgtc cgctacgagc ggcgtcgcga cgcggcgtca ggctgaaccg
      901 gtcccggacg agcccgtcac ggcccgcacg accatcgcac gtcccccacg tccgcgaggc
      961 agacgtccgt gcctggccct ccgccgagcg tgtcgttgcc gcccacctcg tcgacggcgt
     1021 cgagccgatc gtcgccgacg cccccgtcga ggtggtcgcg cccggtgtcc gggaagacgc
     1081 cctgcgccac cccgagggaa tcgtcgatca cgtcgttccc ggcgcgtccg tggaggcggt
     1141 cgtccccgcg gccgccgatc aggcgatcgt tcccgccccg gccgtcgacg acgtccgatc
     1201 cgttgccccc gtcgatcgcg ttgggcagcc gcgagccgag caggacgtcg tcgtacggcg
     1261 atccctcgac cgactcgatc ctcaggagga ggtcgatgcc ttcaccgttc gcccgatgtc
     1321 gcgccagatc ggcgtgcacc gatcggcgag ccaggaagta cgtgatcgaa tcgcgcccac
     1381 ggccgccgtt cagcacatcg ttgccgtcca tgcccaccag ccgatctcgg ccggttcctc
     1441 ccgcgaccga gtcgtccccg gccccggcga tcaggcgatc cttccccggg cctccgtcga
     1501 tcgtgtcgcc gttggctccg ccctcgatcg agtctcggtc tcgcccgccc gacagggagt
     1561 cggcgccgtc gccaccgcag atccggtcgt tccctccgcg tccgacgatc acgtcgttgc
     1621 cgccgaggcc gaggatcgtg tcgctgccgg gggttccgcg gaggacgtcg gggcccggcg
     1681 tcccgacgat cgagccggtt cgggcgaaac agagcggcgc tgccgacgcc ggggggctga
     1741 cggtcgcgat cgccagcgcg gccaccgctg tgaccacgcc tctccggcgt ccccgacccg
     1801 tctcctcccg gtgcatgcat ccctccggac gaccgcggcg attcgatgct cctccgccgg
     1861 gtaccgcggg gtcaaggcgc cgactggact acggtgaccg ggtgcgcggc tcgggggctc
     1921 cgatcgtctc gaacggcgac atcgccgatc tcctgtggcg cgcgagcgac gaggagacga
     1981 cgcatcgccg acgcgcgctc cggaaggcct cgggcgccgc gcgcttctgg agcgaggagg
     2041 ccgccgatct ggccgaagcg ggacggccgc tcacggagct ccgagccgtc ggtccctggg
     2101 tggccgagag gctccaggcg tggctggacg cgccacccga tctcccggag ccggatgcga
     2161 cgaggatcgg gttcctgacg ctcgcggagg tccgggccgt gctcgaccgc gagccggcct
     2221 gggagggtac cccgcacgcc gacctccaga tccatacgac cgacagcgac ggctcgctcc
     2281 cgctccgc
//
LOCUS       DASUVE010000065         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1538919, whole genome shotgun sequence.
ACCESSION   DASUVE010000065 DASUVE010000000
VERSION     DASUVE010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538919"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1241)
                     /locus_tag="VF036_01340"
     CDS             complement(66..1241)
                     /locus_tag="VF036_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020139400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6263905.1"
                     /translation="MSPSELDPTDLIDIDHLLSDEEKQIRGVAREWVQRRILPEVEDW
                     YEKGEFPAHELAKELGELGFFLMQLDTEWTLGLNSVAYGLVEREIEAGDSGLRSFVSV
                     QSALCGHAIHAFGSEEQKNEWLPKMASGEAIACFGLSEPDSGSDPGSMRTSAKKDGDD
                     WILNGTKMWITNGGCSDIAIVWAQTDDGVRGFIVPTDSPGFTANDIHKKLSLRASVTS
                     ELVMDGVRLPADAMLPEVKGLRGPLSCLNEARYGIIWGVMGAARSCFETALEYSKTRE
                     QFDKPIGSFQLTQKKLTDMALELYKGTLLAYHLGRLKDEKGLKPEQVSVGKLNNTREA
                     LEIARTSRGILGANGITLEYPIMRHMTNLESVITYEGTEEVHTLVIGEQLTGLKAFT"
     gene            complement(1473..2210)
                     /locus_tag="VF036_01345"
     CDS             complement(1473..2210)
                     /locus_tag="VF036_01345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017566086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6263906.1"
                     /translation="MDADGYFDESVAATYDEDSAERFDPAIFEPTVDFLAELAGSGSV
                     LEFAIGTGRIAIPLAERGVPVHGIDMSTAMVERLREKPGGNDIAVTIGDFATTRVDGS
                     FSLVYLVYNTIGNLTTQEAQVACFRNAAAHLEAGGTFVIEAGVPELQRLPPGETGHVF
                     NIGEDRWGIDEYDVARQGLISHHFWIQEGRAEHFSMPFRYVWPSELDLMAQLAGMTLR
                     ERWAGWTREPFTSESRSHVSVWEKPTN"
BASE COUNT          360 a          797 c          722 g          405 t
ORIGIN      
        1 ggccaccaca agggtcgtct tccaccagtg accgaagatc agtcccagga ggatgacggt
       61 cgggatcacg tgaacgcttt gaggcctgtg agctgctcgc cgatcaccag cgtatggacc
      121 tcctcggtcc cctcgtaggt gatcacggac tcgaggttcg tcatgtggcg catgatcggg
      181 tactcgagcg tgatgccgtt cgcgccgagg atgccgcgtg aggttcgcgc gatctcgagc
      241 gcctcgcgtg tgttgttcag cttcccgacg ctcacctgct ccggcttcag gcccttctcg
      301 tccttcagcc ggccgaggtg gtaggcgagc aatgtcccct tgtagagctc gagcgccatg
      361 tcggtgagct tcttctgcgt gagctggaac gagccgatcg gcttgtcgaa ctgctcccgc
      421 gtcttcgagt actccagggc ggtctcgaaa caggagcgcg ccgcgcccat cacgccccag
      481 atgatcccgt aacgcgcctc gttcaagcac gagagtggac cgcggagccc cttgacctcc
      541 ggcaacatcg cgtccgccgg cagccgcacg ccgtccatca cgagctccga ggtcaccgac
      601 gccctgagcg acagcttctt gtggatgtcg ttcgcggtga agccgggcga atcggtcggc
      661 acgatgaacc ctcggacccc gtcgtcggtt tgcgcccaca cgatcgcgat gtcggagcaa
      721 ccgccgttgg tgatccacat cttcgtgccg ttcaggatcc agtcgtcgcc gtccttcttc
      781 gcagatgtgc gcatcgaacc gggatcgctc cccgagtcgg gctccgacag gccgaagcac
      841 gcgatcgcct cgccgctcgc catcttcggc agccactcgt tcttctgctc ctcgcttccg
      901 aacgcgtgga tcgcgtggcc gcacagggca ctctgcaccg agacgaacga ccgcaggccg
      961 ctgtcgccgg cctcgatctc gcgctcgacc agcccgtacg ccaccgaatt cagcccgagg
     1021 gtccactcgg tgtcgagctg catcaggaag aagccgagct ccccgagctc cttcgcgagc
     1081 tcgtgcgcag ggaactcgcc cttctcatac caatcttcga cctcggggag gatccggcgc
     1141 tgcacccact cgcgggcgac gccgcggatc tgcttctcct cgtcggagag gaggtggtcg
     1201 atgtcgatca ggtcggtcgg atcgagctcg gacgggctca tggaacgacc gtaccacctc
     1261 gcatcggccg cacctccggg tctctgctcg aggcgtgttc cgctggctga aggcgaggct
     1321 caggcgccgg ggggacgatc tgacccggag cgcgcacgtc gcgcgctcgc cgaccgcgag
     1381 cgagagagcg cggagctctg gaagctccgg acgcctccgt tccctcaaac gccccccgcc
     1441 ggggggtctg tagggcagat cccgccgtcc cgtcagttcg tcggcttctc ccagaccgac
     1501 acgtgggatc ggctctcgct cgtgaacggc tccctcgtcc atccggccca gcgctcgcgc
     1561 agcgtcatcc cggcgagctg cgccatcagg tcgagctcgg agggccacac gtagcggaac
     1621 ggcatcgaga agtgctcggc ccgaccctcc tggatccaga agtgatggga gatcagcccc
     1681 tgcctcgcga cgtcgtactc gtcgattccc cagcgatcct ccccgatgtt gaagacgtgg
     1741 ccggtctcgc cgggcgggag ccgctgcagc tccggcacgc cggcctcgat cacgaacgtg
     1801 ccgccggcct cgaggtgtgc ggccgcgttc cggaagcacg cgacctgcgc ttcctgcgtc
     1861 gtcaggttcc cgatcgtgtt gtagacgaga tagacgagtg agaacgagcc atccacccgg
     1921 gtcgtcgcga agtcgccgat cgtcacagcg atgtcgttgc cacccggctt ctcgcggagc
     1981 cgctcgacca tcgcggtcga catgtcgatg ccgtgcaccg gcacgccgcg ttccgcgagt
     2041 ggaatggcga tccgtccggt cccgatcgcg aactccagaa cggacccgct gccggcgagc
     2101 tccgccagga agtcgacggt cggctcgaag atcgcggggt cgaatctctc ggccgaatcc
     2161 tcgtcgtagg tcgcggcgac ggactcatcg aagtatccgt cggcgtccat cgcgggggaa
     2221 tctaccgcgc gttccttccg gtcagtcctc agaccgaggc cagctcagaa gtcgacgaag
     2281 tggt
//
LOCUS       DASUVE010000066         6193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1162473, whole genome shotgun sequence.
ACCESSION   DASUVE010000066 DASUVE010000000
VERSION     DASUVE010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162473"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VF036_01350"
     CDS             complement(<1..592)
                     /locus_tag="VF036_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX6263907.1"
                     /translation="MDLRPARGTLDMLPPQGSRMRALYDRAAALARLHGYRYVETPGF
                     EATELFSATSGETSDVVTKEMYTFEDRKGRSLTLRPEGTAPVMRAYLGAMQEQPSPFK
                     AYYLTRMYRYARPQEGRYREHRQFGIEVFGTEAPGADAEVIAMGDAFLRGLGLTRIEL
                     QLNSIGDEVCRPAYRDELVAFLRANRDRLHDEHRDRF"
     gene            complement(592..1212)
                     /locus_tag="VF036_01355"
     CDS             complement(592..1212)
                     /locus_tag="VF036_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6263908.1"
                     /translation="MYQDVFDRNPFGTNCWLLGADGGEDSVLVDPGFEPDELRAVLER
                     AGRRPTAILLTHAHLDHAFAAGVLAGDDLPVFVHEADALAFDDIDAWNPGFDNPLEPV
                     KDLRRVVDGDELSFAGMSIVVRHTPGHTPGHCCFVTDATVVAGDLVFAGSIGRSDFPN
                     SSPEAMRTSLRWFLSRDDADDVLPGHGPRTTVGRERSSNPFLVNVA"
     gene            1287..1691
                     /locus_tag="VF036_01360"
     CDS             1287..1691
                     /locus_tag="VF036_01360"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEX6263909.1"
                     /translation="MARHVVLYDNDCGFCRWSLDRLLRWDRARRLRAVPIQGEEGDRL
                     LADLSEEARLASWHLVTPDGRRYSGGAAAGPLARLLPAGAPVALLADTFPRTTDRLYR
                     WVARNRDSLGRRLGEEACAVDPSLRPGRSMPS"
     gene            complement(1678..2133)
                     /gene="dtd"
                     /locus_tag="VF036_01365"
     CDS             complement(1678..2133)
                     /gene="dtd"
                     /locus_tag="VF036_01365"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019239121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX6263910.1"
                     /translation="MRVVLQRVLRASVAADGGTIAEIGRGYLLLVGVGRGDPADEPER
                     LAEKIVNLRLFADDQGKTNRSLADVDGEVLVVSQFTLYADLRKGRRPSWGDAEDPAVA
                     SERVEALAAALEARAVRVGRGVFGATMAVELLNDGPFTLVLDGASLSSA"
     gene            complement(2130..2729)
                     /locus_tag="VF036_01370"
     CDS             complement(2130..2729)
                     /locus_tag="VF036_01370"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6263911.1"
                     /translation="MGSGNWIGYYDAQEGREPRELLTEVLRAFDADGRVGSAVDLGCG
                     DGADSLALLERGWDVLSIDAQDDAIRRLRARLTDDVAARSTTVVSPMQVVDLPRADLV
                     FASFSLPFCPPEAFPDLWARIRASLHPGGRFAGELFGDRDTWASDPEMTFHDVDAARA
                     LFDGLELESFVEEDEDGEAFEGPKHWHVFHVIAKRTDAG"
     gene            complement(2734..5028)
                     /locus_tag="VF036_01375"
     CDS             complement(2734..5028)
                     /locus_tag="VF036_01375"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX6263912.1"
                     /translation="MGSTEQDVPVERVDEEPQPVTPERGRLLPGIPRPRLRRGEQGPP
                     NSLAPVLKRVRSYNPKADTKLLERAYRYAEEAHEGQFRLSGEPYVEHPVAVTGILADL
                     KLDTTTLTAALLHDTVEDTQVGLGDIEQEFGNEVARIVDGLTKLDKLEFRSGELAQAE
                     NVRKMIVAMAGDIRVLLVKLADRLHNMRTLAPLSEERQRKIANETLEIHAPLANRLGV
                     QELKWELEDLAFKTLNPGPYREIANLVESRRGERQQLLEGVKEALRAKLKELGVKADV
                     EGRPKHLYSIYEKMVIRGKEFNEIHDLVGLRVLVPSLRDCYGALGAVHALWKPIPGRF
                     KDYIAMPKSNMYQSLHTTVVGPKGIPLEIQIRTQDMHRTAVFGIAAHWRYKEGSKSSR
                     EAQDMAWLGQMMDWLKDMADPQEFMEGLRIDLSGGRVFCFTPKGDVQNLPQGATPVDF
                     AYSIHTEVGHRTVGAKVNGKLVPLDYELRTGDTVEILASKAQGEGPSQDWLQFVKTPR
                     ARTKIRQWFSRGRREDAMESGRDAVKTFVRRQDQTFKRLASEETIAAVAEDLKYPDVE
                     ALYVAVGEGHVSPQSIVARMSRLVSGTPDEDVTEVPLARPVRIGKPDISKGVVVRGLG
                     DVWVKLGRCCTPVPGDAIVGFVSRGQGVSVHRDTCPNLRTLQRREPERIIDVDWAEGK
                     PTSFSVAIQVEALDRTRLLSDVATVLSDSHVNILAATSSTGRDRITRLRFTFEMADVT
                     HLSSLLAAVKRVESVYDAYRVVPS"
     gene            complement(5103..5624)
                     /locus_tag="VF036_01380"
     CDS             complement(5103..5624)
                     /locus_tag="VF036_01380"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX6263913.1"
                     /translation="MEIEQIKALIRDVPDFPEPGIVFKDITPILAHADAMSTIIDLIV
                     VHFGRGTVDKVVGIEARGFILAAPVAYHFNAGFVPIRKQGKLPWKTASEEYSLEYGTA
                     TLELHRDAIEQGERVLIVDDVLATGGTAQAAVSLVERCGGVIRGISCLIELEFLKGRS
                     KLEDYEVHTFIQY"
     gene            complement(5629..>6193)
                     /locus_tag="VF036_01385"
     CDS             complement(5629..>6193)
                     /locus_tag="VF036_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX6263914.1"
                     /translation="VYALTGREVTPETVIAVLTILGFSLYDTVVIYDKIKENLETQSL
                     VGRLGSDGTIDLSLNQVLMRSVNTSLVVLLPILALLLFGGETLKDFAFAMFIGTAIGV
                     YSSIFLAAPLLAVLKKREARFRQLEQKLTDRERVVARRAATAPAATPGASTGEAAAAR
                     ATARTATTGRPGQKSKRRKPPAKRKRR"
BASE COUNT          923 a         2203 c         2079 g          988 t
ORIGIN      
        1 cgaagcggtc ccgatgctcg tcgtgcaggc ggtcccggtt ggcgcgcagg aacgccacga
       61 gctcgtcgcg gtacgcggga cggcagacct cgtccccgat cgagttgagc tgcagctcga
      121 tgcgggtcag cccgagcccg cgcaggaacg cgtcgcccat cgcgatcacc tcggcgtcgg
      181 cgccgggcgc ctcggtcccg aacacctcga tgccgaactg tcggtgctcg cggtagcgcc
      241 cttcctgcgg ccgggcgtac cgatacatcc tcgtgaggta gtacgccttg aacggagacg
      301 gctgctcctg catcgcgccg aggtaggcgc gcatcaccgg cgcggttccc tccggacgaa
      361 gggtcagcga gcgccccttc cgatcctcga acgtgtacat ctccttcgtg accacgtcgc
      421 tcgtctcacc cgacgtggcg gagaagagct cggtggcctc gaagccgggc gtctccacgt
      481 agcggtagcc gtggagccgg gcgagggccg ccgcgcgatc gtagagcgcg cgcatccgac
      541 tgccctgcgg agggagcatg tcgagggtgc ctcgtgccgg tcgcagatcc atcaggccac
      601 gttcaccagg aacgggttgg acgagcgctc ccgtcccacg gtggtccgcg gaccgtgccc
      661 cggaaggacg tcgtcggcgt cgtccctcga gaggaaccat cggaggctcg tccgcatcgc
      721 ctcgggcgag gagttcggga agtcggagcg accgatcgag ccggcgaaca ccaggtcgcc
      781 ggcaaccacc gtggcgtcgg tcacgaagca gcagtgaccg ggcgtgtgcc cgggcgtgtg
      841 gcgcacgacg atcgacatcc cggcgaacga cagctcgtca ccgtcgacga cgcggcgcag
      901 atccttcacg ggctccaggg ggttgtcgaa cccggggttc caggcatcga tgtcgtcgaa
      961 cgcgagggcg tcggcctcgt ggacgaagac agggaggtcg tcgccggcga gcacgcccgc
     1021 ggcgaaggcg tggtcgagat gtgcgtgcgt caggaggatc gccgtcggtc gccgcccggc
     1081 gcgctcgagg accgctcgga gctcgtccgg ctcgaaaccg gggtcgacga gcaccgaatc
     1141 ctcgcccccg tcggccccga ggagccagca gttcgtgccg aacgggttcc gatcgaagac
     1201 gtcctggtac atctggggca tgctaccggg cgacccggcg cggccgcccc gacgggctcc
     1261 caaccgtggc accgtacgct gacgccatgg cgcgacacgt cgtcctctac gacaacgact
     1321 gtggcttctg ccggtggtcg ctcgatcggc tgctccggtg ggatcgcgcg cgccggctcc
     1381 gggctgtgcc gatccagggc gaggaaggcg accggctcct cgccgatcta tcggaggagg
     1441 cccggctcgc ctcctggcac ctcgtcacgc ccgacggacg gcggtactcg ggcggcgcgg
     1501 cggccggccc gctggctcgg ctcctgccgg ccggcgcccc agtcgctctc ctggccgata
     1561 cgttccctcg caccaccgac cgcctttacc ggtgggtggc gcggaaccgg gactcgctcg
     1621 gccggcggct cggggaagag gcgtgcgccg tcgatccgag cctgcgaccg gggagatcta
     1681 tgccgagctg agcgacgcgc cgtccagcac caacgtgaac ggaccgtcgt tgaggagctc
     1741 cacggccatc gtcgcgccga acacaccgcg tcccacgcgc accgcgcggg cctcgagcgc
     1801 cgcggcgagc gcttcgaccc gctccgaggc gaccgccgga tcctccgcat ccccccagga
     1861 cggacgacgc cccttccgga ggtccgcgta cagggtgaac tgggacacca cgaggacctc
     1921 gccgtcgacg tctgccaggg aacggttcgt cttcccctgg tcgtcggcga acagacgcag
     1981 gttcacgatc ttctccgcca gccgctcggg ctcatccgcc ggatcgccgc gcccgacgcc
     2041 gaccaggagc aggtagccac gtccgatctc agcgatggtt cctccgtccg ccgccacgga
     2101 cgcgcggagt acccgctgca acacgaccct catccggcat ccgtccgctt cgcgatcacg
     2161 tggaacacat gccagtgctt cggcccctcg aaggcttcgc cgtcctcgtc ctcctcgacg
     2221 aaggactcga gctcgagccc gtcgaacagc gcacgagcgg cgtcgacgtc gtggaacgtc
     2281 atctcgggat cggatgccca cgtgtcgcga tcgccgaaca gctcacccgc gaagcggcca
     2341 cccgggtgga gcgacgcccg gatccgagcc cacaggtccg ggaacgcttc cggcggacag
     2401 aacgggaggc tgaagctcgc gaacacgagg tccgcccggg gcagatcgac gacctgcatc
     2461 ggggacacaa cggtggtcga ccgcgcggcc acgtcgtccg tgagccgcgc gcggagacgc
     2521 cggatcgcgt cgtcctgggc gtcgatcgac agcacatccc agccccgctc gaggagggcc
     2581 agcgagtcgg ctccgtcgcc gcacccgaga tcgacggcgc tccccacccg gccatcggca
     2641 tcgaacgccc ggagaacctc cgtcagcagc tcgcgaggtt cgcgaccttc ctgagcgtcg
     2701 tagtagccga tccagttccc ggagcccact cccctagctc gggacgacgc ggtaggcgtc
     2761 gtagacgctc tccacccgct tcaccgccgc cagcagcgac gagaggtgcg tcacatcggc
     2821 catctcgaac gtgaaccgga ggcgcgtgat gcggtcccgc cccgtgctgg aggtggcggc
     2881 gaggatgttg acgtggctgt cggacaggac ggtggccacg tcggagagca gccgcgtccg
     2941 gtcgagggcc tccacctgga tcgccaccga gaaggacgtg ggcttgccct ccgcccagtc
     3001 cacgtcgatg atccgctccg gctcacgccg ctggagcgtc cggagattgg gacacgtgtc
     3061 gcggtggacg gagacgccct ggccgcggga gacgaatccc acgatcgcat cccccggcac
     3121 cggcgtgcag caccgtccga gcttcaccca gacgtccccc aaacctcgca ccacgacgcc
     3181 cttggagatg tcgggcttcc cgatccgcac cgggcgggcg agcgggacct ccgtcacgtc
     3241 ctcgtccggt gtcccggaga ccaggcgtga catccgcgcg acgatcgact gcggagacac
     3301 atgcccctcc ccgaccgcga cgtacagggc ctcgacgtcc gggtacttca ggtcctccgc
     3361 gacggccgcg atcgtctcct cggacgcgag gcgcttgaac gtctgatcct gccggcgcac
     3421 gaacgtcttg acggcatcgc gcccggactc catcgcgtcc tcgcggcggc cacgactgaa
     3481 ccactggcgg atcttcgtcc tggcccgtgg cgtcttcacg aactggagcc agtcctggct
     3541 cggtccctcg ccctgcgcct tcgacgcgag gatctcgacg gtgtccccgg tgcggagctc
     3601 gtagtcgagc gggacgagct tcccgttcac cttggccccc accgtgcggt ggccgacctc
     3661 cgtgtggatc gagtacgcga agtccaccgg tgtggcgcct tgcggcaggt tctgcacgtc
     3721 gcccttcggg gtgaagcaga agacccgccc gcccgacaga tcgatccgca gaccctccat
     3781 gaactcctgc ggatcggcca tgtccttcag ccaatccatc atctggccga gccaggccat
     3841 gtcctgtgct tccctcgagg acttgctgcc ctccttatag cgccagtgcg cggcgatgcc
     3901 gaacacggcc gtgcggtgca tgtcctgcgt ccggatctgg atctccaggg ggatgccctt
     3961 cggtcccacc acggtcgtgt gcaacgactg gtacatgttc gacttcggca tcgcgatgta
     4021 gtccttgaaa cggcccggga tcggcttcca gagcgcgtgg acggcaccga gggcgccgta
     4081 gcagtcgcgc agcgagggca ccaggacgcg gagaccgacg aggtcgtgga tctcgttgaa
     4141 ctccttgccg cggatcacca tcttctcgta gatggaatag aggtgcttcg ggcgaccctc
     4201 gacgtccgcc ttcacgccga gctccttgag cttcgcccgg agggcctcct tgacgccctc
     4261 caggagctgc tgccgctctc ctctgcgact ctcgacgagg ttcgcgatct cgcggtaggg
     4321 acccgggttg agcgtcttga acgcgaggtc ctcgagctcc cacttcagct cctgcactcc
     4381 caggcggttc gcgagcggcg cgtggatctc gagcgtctcg ttcgcgatct tgcgctggcg
     4441 ctcctctgag agcggcgcga gggtgcgcat gttgtggaga cggtccgcga gcttgacgag
     4501 caggacccgg atgtcgcccg ccatcgcgac gatcatcttg cggacgttct ccgcctgcgc
     4561 gagctcgccg ctgcggaact cgagcttgtc gagcttcgtc agtccgtcga cgatgcgagc
     4621 gacctcgttg ccgaactcct gctcgatgtc cccgaggccc acctgggtgt cctcgaccgt
     4681 gtcgtgcagg agggccgccg tgagcgtcgt ggtgtccagc ttcaggtccg cgagtatgcc
     4741 ggtcacggcc accggatgct cgacgtaggg ctccccggag aggcggaatt ggccctcgtg
     4801 ggcctcctcc gcgtagcggt aggctcgctc gagcagcttc gtgtcggcct tggggttgta
     4861 ggaacggacc ctcttcagga cgggggccag gctgttcggg ggtccttgct cgccccgtcg
     4921 gagcctgggc cgagggatcc ccgggagcag ccggccgcgc tcgggcgtga cgggctgcgg
     4981 ctcttcatcg acccgctcga ccggtacgtc ctgctcggtg ctgcccacgc tcgcctcctg
     5041 caccaagtgt aggggacgcc gtctgtggag ctcgggagca caccggtcga gccgtgccgg
     5101 gctcagtact ggatgaacgt gtggacctcg tagtcctcga gcttcgagcg ccccttcagg
     5161 aactcgagct cgatcaggca cgagatcccg cggatcacac cgccgcagcg ttcgacgagc
     5221 gagaccgccg cctgtgccgt cccgccggtg gccaagacgt cgtcgacgat caggacgcgc
     5281 tcgccctgct cgatcgcgtc ccggtggagc tcgagcgtgg ccgttccgta ctcgagtgag
     5341 tactcctcgg acgccgtctt ccaggggagc ttgccctgct tgcggatcgg cacgaagccg
     5401 gcgttgaagt ggtacgccac cggggccgcg aggatgaacc cacgggcctc gatcccgacg
     5461 accttgtcga cggtgccccg gccgaagtgg acgacgatca ggtcgatgat cgtcgacatc
     5521 gcgtcggcgt gcgccaggat cggcgtgatg tccttgaaga cgatgcccgg ttccgggaag
     5581 tcgggcacgt ccctgatcag cgccttgatc tgctcgatct ccacgacctc acctccgctt
     5641 gcgcttggcc ggcggcttgc gccgcttcga cttctgtccg ggtcgcccgg tcgtcgcggt
     5701 ccgggcggtc gcgcgggcgg cggccgcctc gccggtgctc gcgccgggcg tggccgcggg
     5761 cgcggtcgcc gcccgccgcg ccaccacccg ttcccggtcc gtgagcttct gctcgagctg
     5821 tcggaatcgc gcctcccgct tcttcagcac cgcgaggagc ggcgcggcga ggaagatcga
     5881 cgagtacacg ccgatcgccg tgccgatgaa catcgcgaac gcgaagtcct tgagcgtctc
     5941 gccaccgaag agcagcagtg ccaggatcgg gagcaggacc acgagggacg tgttcaccga
     6001 tcgcatcagc acctggttga gggagagatc gatcgttccg tccgacccga gcctccccac
     6061 caacgactgc gtctcgaggt tctccttgat cttgtcgtag atcacgaccg tgtcgtagag
     6121 ggagaacccg aggatcgtga gcaccgcgat cacggtctcc ggcgtcacct cccgtcccgt
     6181 cagcgcatac acg
//
LOCUS       DASUVE010000067         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_32464, whole genome shotgun sequence.
ACCESSION   DASUVE010000067 DASUVE010000000
VERSION     DASUVE010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32464"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..700
                     /locus_tag="VF036_01390"
     CDS             71..700
                     /locus_tag="VF036_01390"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX6263915.1"
                     /translation="MFAGIVEELGTVVARDGERLVVRGAVTSADATIGDSLAIDGVCL
                     TVVDLGAEQLSFDLSEETLARTNLGSRGPGDAVNLERPATLASRIGGHLVQGHVDGVA
                     EVTGVRPEGEDGIRLSVRLPRELLRHVVEKGSITLDGVSLTVAALDGEEIEIALIPHT
                     LRVTTLGSVRESDRVNVEVDIVAKYVARNLEYLTADGAAVVRPRKERTA"
     gene            697..1965
                     /locus_tag="VF036_01395"
     CDS             697..1965
                     /locus_tag="VF036_01395"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX6263916.1"
                     /translation="MSALSETSVFASIPEAIDEIRQGRMVIVVDGADRENEGDLIMAA
                     ERVTPEAIAFIVRHTSGVICMPVIGERLDELEIPLMVAANTDSRRTAFTVSVDARMGV
                     TTGISAADRARTIRAIIDPETEPGDLSRPGHVFPLRYREGGVLKRAGHTEAAVDLARL
                     AGLFPAGVLCETVNEDGTMARLPDLERFARAHELKIISIADLIRHRRQYEVLVHEVAE
                     ANIPTRHGEFRSIAYESVTDARTHVALVMGDIGDGENVLTRVHSECLTGDVFGSLRCD
                     CGGQLDRAMELIGREGRGVILYIRGHEGRAIGITHKLRAYALQDRGRDTVEANLELGF
                     PADQRDYGIGAQILYDVGVRSMRLLTNNPEKRAGLEGHGLAINERLPLQTDPTPQNVG
                     YLRAKREKLGHLLDLPDVDGPEIDEVEARA"
     gene            1962..>2220
                     /gene="ribH"
                     /locus_tag="VF036_01400"
     CDS             1962..>2220
                     /gene="ribH"
                     /locus_tag="VF036_01400"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009373785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX6263917.1"
                     /translation="MTVMRGAPEGAGRRVGVVVARFHEHVTAKLLEGALGGLRARGVA
                     DDAIDVAWVPGAFEIPLVVRRLASTGAYDALICLGAVVRGET"
BASE COUNT          347 a          737 c          809 g          327 t
ORIGIN      
        1 ctcgcggagg cgtcgccgct cgacatcgtg tccgtggaac gcctcgatcg ggacctgaag
       61 gtggtggccg atgttcgccg ggatcgttga ggagctcgga accgtcgtcg cgcgcgatgg
      121 ggagcgcctg gtcgttcgcg gtgcggtcac gtccgcggac gcgaccatcg gcgattcgct
      181 cgcgatcgac ggcgtttgcc tcacggtcgt cgacctcggg gcggagcagc tctccttcga
      241 cctctcggag gagacgttgg cgcggacgaa cctcggctcg cgcggtcccg gggacgcggt
      301 gaacctggaa cgaccggcta cgctcgcttc tcgcatcggg ggccatctcg tgcagggaca
      361 cgtggatggt gtcgccgagg tgaccggcgt ccgacccgag ggggaggacg gcatccgact
      421 ctcggtgcga ctccctcggg agctgctccg ccacgtcgtg gagaaggggt cgatcacgct
      481 cgacggcgtg agcctgacgg tggcggccct cgacggtgag gagatcgaga tcgcgctgat
      541 cccgcacacc cttcgcgtca ccacgctggg ctcggtccgc gagagcgacc gggtcaacgt
      601 ggaggtggac atcgtcgcca agtacgtggc ccggaacctc gagtacctca ccgccgacgg
      661 cgcggccgtc gtccgaccga gaaaggaacg aaccgcatga gcgcgctgag cgaaacgagc
      721 gtcttcgcct ccatccccga ggcgatcgac gagatccgcc agggtcggat ggtgatcgtc
      781 gtcgacggcg cggaccggga gaacgaaggc gatctgatca tggccgcgga gcgcgtgacg
      841 cccgaggcga tcgcgttcat cgtccgccac acgagcgggg tgatctgcat gcccgtgatc
      901 ggtgaacgac tcgacgagct cgagatccca ctgatggtgg cggcgaacac cgattcccgg
      961 cgaaccgcgt tcacggtgtc cgtcgacgcg cggatgggcg tcacgacggg catctcggcc
     1021 gcggatcggg cacgcacgat cagggcgatc atcgatcccg agaccgagcc gggcgacctg
     1081 tcgcggccgg gtcacgtctt ccccctccgg tatcgggaag gcggtgtcct caagcgggcg
     1141 ggacacacgg aggccgccgt cgacctcgcg cggctggccg gcctcttccc ggccggcgtc
     1201 ctgtgcgaga ccgtcaacga ggacggcacg atggcgcggc tcccggatct ggagcggttc
     1261 gcccgggcgc acgaactgaa gatcatctcg atcgccgatc tgatccgcca tcgccgccag
     1321 tacgaggtcc tcgtgcacga ggtggccgag gcgaacatcc ccacgcggca cggtgagttc
     1381 cgatcgatcg cgtacgagag cgtgaccgac gcgcggacgc acgtcgcgct cgtgatgggc
     1441 gacatcggtg acggcgagaa cgtcctgacg cgcgtccact ccgagtgcct cacgggggac
     1501 gtgttcgggt ccctgcgctg tgactgcggg gggcagctcg accgagcgat ggagctgatc
     1561 ggtcgtgagg ggcgcggggt gatcctctac atccgcggac acgagggccg cgcgatcggc
     1621 atcacgcaca agctccgcgc gtacgccctc caggatcgcg gtcgcgacac ggtggaggcg
     1681 aacctcgagc tcgggttccc ggccgatcaa cgcgactacg gcatcggcgc ccagatcctg
     1741 tacgacgtcg gcgtccggtc catgcgactg ctcacgaata acccggagaa gcgggcgggc
     1801 ctcgaggggc acggcctcgc gatcaacgag cggctgccac tacagacgga tccgacgccg
     1861 cagaacgtcg gctacctccg ggccaagcgg gagaagctcg gtcatctcct ggatctcccc
     1921 gacgtggacg ggccggagat cgacgaggtg gaagcgcgag catgacggtg atgagaggcg
     1981 ccccggaggg cgcggggcgc cgcgtcggcg tcgtcgtcgc ccggttccac gagcacgtga
     2041 ccgcgaagct cctggagggc gcgctcgggg ggctccgtgc ccgcggcgtc gccgatgacg
     2101 cgatcgacgt cgcctgggtt cccggtgcct tcgagatccc gctcgtggtc cggcggctgg
     2161 cctccaccgg cgcctacgac gcactgatct gtctcggcgc cgtggtccgg ggcgaaacgg
//
LOCUS       DASUVE010000068         5379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_785300, whole genome shotgun sequence.
ACCESSION   DASUVE010000068 DASUVE010000000
VERSION     DASUVE010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785300"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2367)
                     /locus_tag="VF036_01405"
     CDS             complement(<1..2367)
                     /locus_tag="VF036_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6263918.1"
                     /translation="MSQAGTAMREERRFVTALFADLVGSTALGERLEPEELKLVVGDA
                     VGRIVGAVEAFGGTIKDLAGDGVLALFGAPVAHEDDPERALLAGLRIVEEIGEFADEV
                     RRSWGIDGFGVRVGVESGPVVVGAVGAGSHVEYGAMGDAVNVAARLQAKAGPGTILVG
                     EGTAARVDPLFEWGPPVQLELKGKSGSVAARPVIAPMATSVSTRGIEGVQAPVVGRER
                     ELAVVRELARAALEGTGAIAVMTGEPGIGKTRLLSEFRALFVEGSAPQGRPLWLEGKC
                     VSYGESMTYWPFRDLVRSWLGVLADEPEMRVRVALRRNVERLFGDRAAELTPYLAAML
                     GLTLEPDDQTRLGELSPEALQYRTFEVVRHWLQRLAEDGPVAVALEDLHWADATSLQL
                     LERLLPDTEDSALLLVLTLRQERDHPAWRLKDDAARELPHRTTEMELEALSGDAGLDL
                     LRSLVGTGTLPEDMEARILEHADGNPFFLEELVRSLADAGSLVRDGDGWRYDHEADVE
                     IPPTVEKVILARIDRLDPQPHATLMAASVLGREFGLPLLEAVTGDGDVKESLSSLMRL
                     DLLREARRWPEPEYRFKHALIQEAAYRTLVVDERRALHSKAATWLESRYAGREEEVAG
                     LLAHHWLAAADEDKAVAYLTMAGDRARQEYALDEAIASYRELLPILGERGEQDAIALV
                     LFKLALAFHMSLRFAEANEMYQRAFDLWRRPDPATEPPSATLRVGTSFLPNDPDPRSA
                     IAWPNIQLCMQLFDRLVEAWPERTIVPSLAERWEISDDGLRYVFHLREG"
     gene            2444..4924
                     /locus_tag="VF036_01410"
     CDS             2444..4924
                     /locus_tag="VF036_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6263919.1"
                     /translation="MTERMLDDTLTAASFAPDAADAPLPDRAQVVIVGGGIAGSSIAY
                     HLAGLGITDVLLLERARIASGTSWHAAGLVARVRGSHPMTELANYGVDLYRGLADETG
                     VDVHFRPSGSLTLAENEGRKTELRYAAAIARHHGTESRLLEPPEVPEVWPLISTDGLV
                     GALLQPGDGTVNPGYAALALAKGAHDGGATIREGVRVEELLLDGRTMTGVRTDRGDVE
                     AETVVLACGLWTRDLAATVGVPVPLYAAEHVHVSTEPIEGAHDLLPLVRGLDGSFYAR
                     HHAGGLMIGAFEPNGRPRSTVSIPDEWAFGEFGPDWEHFAPTRANATRRIPALEEAEF
                     TRFLRAPESFTPDVNFCLGETAEVRNLFVAAGFNSQGIIYSPGAGRALAEWIREGAPT
                     FDASEVDVRRFASSQADERYLYERTKEALGRLYAMHWPNLQATTARGIRRSPLYDRLE
                     AAGACFGELTQWERPMWFAPTGVGPEYGYSFGRQNWFPYSAEEHRAAREAVALFDLSS
                     FAKIEVTGANALSLLQRVCTNDIDIGVGRALYTLVLNPRGGIENDGTVTRLAADRFLV
                     ITPTATQHRTLEWLRAEAHAMTVSIDDVTSALATIALMGPRSRDLLGRLTTADLSNEG
                     FPFFSAQEMQVASAPVLAIRASFVGELGWELYVPSEFAVHVYDAVIERGQGLGLRHAG
                     YHALDSLRIEKGFVHVGHDVGPTDDPFSAGLGHVVKLEKDFIGADGARRAEGSAGTHR
                     LVSLTLEDPEPVLLHGESVIADGKIVGTVMSAAYAHTIGAALGLAMVDASVVENEAPV
                     QVDIAGEVVKATLSVRPLYDPSGARMRS"
     gene            4944..>5379
                     /locus_tag="VF036_01415"
     CDS             4944..>5379
                     /locus_tag="VF036_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263920.1"
                     /translation="MPPKQVALRRRVGAFAVALLAVVAGFFLWPSQDAAPVRSDLGEG
                     PSSSYSGPDNYLAWVPGGFDDPAFRQKMERLPGLDEAVVVAGDTLWLRRTEDAEGLVV
                     DEPKSPFAFPIDAFAVDANDYAPFVGTSVREEIVRALGADQAV"
BASE COUNT          793 a         1912 c         1864 g          810 t
ORIGIN      
        1 gccctcgcgg aggtggaaga cgtagcggag gccgtcgtcg gagatctccc accgctccgc
       61 gagcgaaggc acgatcgtcc gctccggcca cgcctcgacc agccggtcga acagctgcat
      121 gcagagctgg atgttcggcc acgcgatcgc cgatcgggga tcggggtcgt tcgggaggaa
      181 gctggtgccc acgcgcagcg tcgcggacgg cggctcggtc gccggatccg gccggcgcca
      241 gagatcgaac gcccgctggt acatctcgtt cgcttccgcg aagcggagcg acatgtggaa
      301 cgccagcgcg agcttgaaca ggacgagcgc gatggcgtct tgctcgccgc gttctcccag
      361 gatcggcagg agctcccggt agctcgcgat cgcttcgtcg agcgcgtact cctgccgtgc
      421 gcggtcgccg gccatcgtca ggtacgcgac cgccttgtcc tcgtcggcgg cggcgagcca
      481 gtgatgcgcc agcagcccgg cgacctcctc ctcgcggccg gcgtagcggg actcgagcca
      541 tgtggcggcc ttcgagtgga gcgcgcgtcg ctcgtccacg acgagcgtcc ggtacgccgc
      601 ctcctggatc aacgcgtgct tgaaccgata ttccggttcc ggccaccgtc gcgcctctcg
      661 gagcagatcg aggcgcatca gggatgagag cgactccttc acgtcgccgt cgccggtcac
      721 tgcttccagg agcggcagcc cgaactcccg ccccaggacc gatgccgcca tcagcgtggc
      781 gtggggctgg ggatccaggc gatcgatgcg cgcgaggatg accttctcga cggtcggcgg
      841 gatctctaca tcggcttcgt ggtcgtaccg ccagccgtcg ccgtcccgga ccagtgaacc
      901 cgcgtcggcc aacgagcgga ccagctcctc gaggaagaac gggttgccgt cggcgtgctc
      961 gaggatccgc gcctccatgt cctcgggcag cgtgcccgtc ccgacgagcg atcgcagcag
     1021 gtcgaggccg gcgtcacccg agagcgcttc gagctccatc tccgtcgtgc ggtgcggaag
     1081 ctccctggcg gcgtcgtcct tgagccgcca ggcggggtgg tcgcgctcct gccggagcgt
     1141 gaggacgagg aggagcgcag agtcctcggt gtcggggagc agccgttcga ggagctgcaa
     1201 cgacgtggcg tcggcccagt gcagatcctc gagggcgacc gccaccgggc cgtcctcggc
     1261 gaggcgctgc aaccagtgcc gtacgacctc gaacgtccgg tattggaggg cctccggaga
     1321 gagctcgccg agacgcgtct ggtcgtccgg ctcgagcgtg agcccgagca tcgcggcgag
     1381 atacggggtg agctcggcgg cgcgatcgcc gaagagccgc tcgacgttgc gacggagggc
     1441 cacccggacc cgcatctccg gctcgtccgc gaggaccccg agccacgaac ggacgaggtc
     1501 ccggaacggc caataggtca tcgactcccc gtaggacacg cacttgccct ccagccagag
     1561 cgggcgacct tgcggcgccg agccctcgac gaacagggcg cggaactccg agaggagcct
     1621 ggtcttgccg atccccggct caccggtcat gaccgcgatc gctccggtgc cctcgagagc
     1681 ggctcgcgcg agttcccgga cgaccgcgag ctcacgctcc cgcccgacga caggcgcctg
     1741 cacgccctcg atccctcggg tcgacaccga cgtcgccatg ggagcgatca cgggtctcgc
     1801 ggcgaccgag cccgacttcc ctttcagctc gagctgcacc ggaggtcccc actcgaagag
     1861 cggatccacg cgagcggcgg tgccttcccc gacgaggatc gtgcccggcc cggccttcgc
     1921 ctggagtctc gccgccacgt tcactgcgtc gcccatcgcc ccgtactcca cgtgcgaacc
     1981 cgcgccgacc gcccccacca ccacgggacc ggactccacg ccgacccgga cgccgaaccc
     2041 gtcgatcccc cagctccggc ggacctcgtc ggcgaactcg ccgatctcct ccacgatccg
     2101 gagtccggcg aggagcgctc gctcggggtc gtcctcgtgg gccaccgggg cgccgaagag
     2161 cgcgagcacg ccgtcgcccg ccaggtcctt gatcgtgccg ccgaaggcct cgaccgctcc
     2221 cacgatccga ccgaccgcgt cgccgaccac gagcttcagt tcctccggct cgagccgctc
     2281 cccgagggcc gtcgaaccga cgaggtcggc gaacagcgcg gtcacgaacc gccgctcttc
     2341 ccgcatcgcc gttcccgcct ggctcacggg tgcatcgtcc caaccggctc gacccgagac
     2401 aaccgatggg aggcgcgtag agcgccggta tccttcgggc ctcatgacgg agcggatgct
     2461 tgacgacacc ctcaccgcgg cgtcgttcgc tccggatgca gcggacgcac ctctccccga
     2521 ccgcgcccag gtcgtgatcg tcgggggcgg gatcgcgggg tcgtcgatcg cgtatcacct
     2581 ggcgggactc gggatcacgg acgtgctgct cctggagcga gctcggatcg catccggcac
     2641 ctcgtggcat gccgcggggc tcgtcgcccg ggtccgcggc tcccatccga tgacggagct
     2701 cgcgaactac ggcgtggatc tctatcgagg actcgccgac gagacgggcg tcgacgtgca
     2761 cttccggccg agcggctcgc tcacgctcgc cgagaacgag ggcaggaaaa cggagcttcg
     2821 gtacgcggcc gcgatcgccc gtcatcacgg gaccgagtcc cgactcctgg aaccccctga
     2881 ggtgcccgag gtgtggccgc tgatctcgac cgacggtctc gtcggcgccc tcctccagcc
     2941 cggcgacggg accgtgaatc cgggatacgc ggcgctcgcc ctcgccaagg gcgcgcatga
     3001 cggcggcgcc acgatccgcg agggcgtccg ggtcgaggag ctgctcctcg atggccggac
     3061 gatgacgggc gtccgcaccg atcgaggcga cgtggaggcc gagacggtcg tcctcgcctg
     3121 tggtctgtgg acgcgtgacc tcgccgccac cgtcggcgta ccggtgccgc tctacgcggc
     3181 cgagcacgta cacgtctcga ccgagccgat cgagggcgcg cacgacctgc tgccgctcgt
     3241 gcgcggactc gacggatcct tctacgcgcg gcatcacgcc ggcgggttga tgatcggcgc
     3301 gttcgaaccg aacggacgcc ctcgctcgac cgtatcgatc cccgacgagt gggcgttcgg
     3361 ggagttcggc cccgactggg agcacttcgc tccgacgcga gcgaacgcga cgcggcggat
     3421 ccctgcgctc gaagaggcgg agttcacgcg cttcctccgc gcccccgagt ccttcacccc
     3481 ggacgtgaac ttctgtctcg gcgaaacggc cgaggtacgg aacctcttcg tggccgccgg
     3541 gttcaactcg caggggatca tctactcgcc gggggcgggt cgcgccctcg ccgagtggat
     3601 ccgggagggc gctcccacgt tcgacgcctc cgaggtcgac gttcggcggt tcgcgtcgtc
     3661 gcaggcggac gagcgctacc tctatgagcg cacgaaggag gcgctcggcc gtctctacgc
     3721 gatgcactgg ccgaacctcc aggcgacgac ggcgcgcggc atcaggcgtt ctcccctgta
     3781 cgaccgtctc gaggccgccg gtgcgtgctt cggggagttg acgcagtggg agcgcccgat
     3841 gtggttcgcg ccgaccggcg tcgggccaga atacgggtat tccttcgggc ggcagaactg
     3901 gttcccgtat tcggccgagg agcaccgcgc cgcgcgggaa gccgtcgcgc tcttcgacct
     3961 gagttcgttc gccaagatcg aggtgacggg cgcgaacgcc ttgtccctgc tccaacgggt
     4021 gtgcacgaac gacatcgaca tcggcgtcgg gcgcgccctc tataccctcg tcctgaaccc
     4081 ccgtggtggg atcgagaacg atgggacggt cacacgcctg gccgccgacc gattcctggt
     4141 gatcacgccg acggcgacgc agcaccggac gctcgagtgg ctccgagctg aagcccacgc
     4201 gatgaccgtc tcgatcgacg acgtgacgag cgcgctcgcc acgatcgccc tgatgggacc
     4261 acggagccgg gatctgctgg gacgcctcac cacagccgac ctctcgaacg agggcttccc
     4321 gttcttctcg gcgcaggaga tgcaggtcgc gagcgcgccg gtcctcgcga tccgagcctc
     4381 gttcgtcggt gagctcggct gggagctgta cgtcccgtcg gagttcgccg tgcacgtgta
     4441 cgacgcggtg atcgagaggg gacaggggtt gggcctccga cacgccggct accacgcgct
     4501 cgactcgctg cggatcgaga agggcttcgt gcacgtcggc cacgacgtcg gtccgaccga
     4561 cgacccgttc tccgccggac tcgggcacgt cgtgaagctg gagaaggact tcatcggagc
     4621 cgacggggct cgccgcgccg aggggtcggc ggggacgcat cgactcgtga gcctcacgct
     4681 ggaggaccca gagccggtcc tccttcacgg cgagtcggtg atcgcggacg ggaagatcgt
     4741 gggcacggtg atgtccgcgg cctacgcgca cacgatcggc gcggccctcg ggctggccat
     4801 ggtggatgcc tcggtcgtcg agaacgaggc gccggtccag gtggacatcg caggcgaggt
     4861 ggtgaaggcc acgctctccg tccgtccgct ctacgacccg agcggtgctc ggatgcgttc
     4921 ctgacccgaa gtagcctgaa gacgtgcccc caaagcaggt cgccctccgg cggcgcgtcg
     4981 gcgcgttcgc cgtcgcgctc ctcgccgtcg tcgccgggtt cttcctctgg ccgtcgcagg
     5041 atgcggcgcc ggttcgctcg gacctcggcg agggaccgag ttcgtcctat tcggggcccg
     5101 acaactacct cgcatgggtc ccgggtggct tcgacgatcc cgccttccgc cagaagatgg
     5161 aacgcctgcc cggcctcgac gaggcggtgg tggtcgccgg cgacacgctg tggctccgca
     5221 ggacggagga tgccgaggga ctggtggtgg acgagccgaa gagcccgttc gcgttcccga
     5281 tcgatgcctt cgcggtggat gcgaacgact acgcaccgtt cgtcggcacg agcgtccgcg
     5341 aggagatcgt tcgagcgctc ggtgccgatc aggcggtgt
//
LOCUS       DASUVE010000069         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_597782, whole genome shotgun sequence.
ACCESSION   DASUVE010000069 DASUVE010000000
VERSION     DASUVE010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597782"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VF036_01420"
     CDS             complement(<1..201)
                     /locus_tag="VF036_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263921.1"
                     /translation="MPMWIVVLTALLGAFVIWRVGIAMLRSLTLSSPTSASDEPAPSE
                     PEDVDALDVYLVCSECGTELKVS"
     gene            complement(220..2028)
                     /locus_tag="VF036_01425"
     CDS             complement(220..2028)
                     /locus_tag="VF036_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6263922.1"
                     /translation="MTPPWERRFRAPVITYPDWSPAAPSRLAYESTESGVWQLHVADL
                     DSGVRRRVTDHPVGVVSGAWSPDGADICWWQDETGDESGRWYAQAFGGGDPRLLFPEL
                     DRGWNEGIAWVPGILAASISAHDAFAVYVSEDGGATVKEVARSTEHMRVAGRQGGYNR
                     GGLSADGSLLCLEHAEHGDEMHEALRVIDPRSGATVAELVDDGKQLLTSAWSPVVGDR
                     RLAVVHERRGIPQPALWDPVTGDWTEIVTGLEGEVSVADWWPDASALLLSQLLDGRHR
                     LHRFELATGTWSGIEHPEGQVEWAQVRPDGAVWCRHSSGASSPRILDEAGGEVLPLQG
                     APAPAGRPFELLRFDNRHGQSVQGFLVTPAGSGPWPILIRPHGGPTWLDEDKWHPEVQ
                     SYVDAGLAVVLVNYRGSTGRGAEWRDTLVGDVGGPELEDLNDALDHLVAAGIADPERV
                     AVGGWSWGGYLTLMMLGKHPERNWRCGIAGVPVGDYELSYEDMSPLLQAYDRALLGGR
                     PDEVPDLMADRNPINSADDVEAPVLFLIGEHDSRCPYRQAMAYVDRLRARDHPHEVYL
                     FATGHGSFDIEEEIRQQRAILGFLAEHVSGVAVPTD"
     gene            complement(2054..>2264)
                     /locus_tag="VF036_01430"
     CDS             complement(2054..>2264)
                     /locus_tag="VF036_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263923.1"
                     /translation="KTFEILDPISLWTGIGWGVVLATAAAICTASTLRIHPERWGALL
                     VARAGLIGAMAGGTALFVRLIAALD"
BASE COUNT          353 a          839 c          764 g          308 t
ORIGIN      
        1 cgagaccttg agctcggtcc cgcactcgct gcacacgaga tagacgtcga gggcgtcgac
       61 gtcctcgggc tccgaggggg cgggctcgtc ggaggccgac gtcggagagg acagcgtcag
      121 agaccgcaac atcgcgatgc cgacgcgcca gatcacgaac gcgccgagca gcgccgtcag
      181 cacgacgatc cacatgggca cggagcccac gatagcgact cagtccgttg ggacggccac
      241 gccggacacg tgctcggcga ggaaaccgag gatcgcccgc tgctgacgga tctcctcctc
      301 gatgtcgaag gagccgtgcc cggtggcgaa gaggtacacc tcgtgcggat ggtcccgcgc
      361 ccgcagacgg tccacgtagg ccatcgcctg ccggtagggg cagcgggaat cgtgctcgcc
      421 gatcaggaac aggacgggcg cttccacgtc gtccgcggag ttgatcgggt tccggtcggc
      481 catcaggtcg ggcacctcgt ccggtcgtcc gcccaacagc gcgcggtcgt acgcctggag
      541 cagcggcgac atgtcttcgt aggagagctc gtagtccccg accggcacgc cggcgatgcc
      601 gcaacgccag ttccgttcgg gatgcttccc cagcatcatc agcgtgaggt acccgcccca
      661 cgaccaaccg cccacggcca cgcgttccgg atccgcgatc cccgccgcga ccagatggtc
      721 gagcgcgtcg ttgaggtcct ccagctcggg tccgccgacg tcgccaacga gggtgtcacg
      781 ccactccgcg ccccgaccgg tcgagccccg gtagttgacg agcacgacgg cgagtccggc
      841 gtcgacgtag gactggacct cgggatgcca cttgtcctcg tcgagccagg tcgggccccc
      901 gtgcggacgg atcaggatcg gccacggccc gctccccgcc ggcgtcacga ggaacccctg
      961 gacggactga ccgtgtcgat tgtcgaatcg cagcagctcg aacggcctgc cggccggtgc
     1021 cggcgcgcct tggagtggga gcacctcgcc gccggcctcg tccaggatcc gcggactcga
     1081 ggcgccgctc gagtgacggc accagacggc gccgtccggc cgcacctgcg cccactcgac
     1141 ctgaccctcg ggatgctcga tccccgacca cgtccccgtg gcgagctcga accggtggag
     1201 ccggtgccgt ccgtcgagga gctgcgagag caggagcgcg ctcgcgtccg gccaccagtc
     1261 cgcgaccgag acctctccct cgagtccggt cacgatctcc gtccagtccc cggtcacggg
     1321 atcccacagc gcgggctgcg ggatcccgcg ccgttcgtgc acgacggcga gacggcggtc
     1381 cccgacaacg ggcgaccatg cagaggtaag gagctgcttc ccgtcgtcga ccagctcggc
     1441 gaccgtcgct cccgaccgtg ggtcgatcac ccgaagtgcc tcgtgcatct catcgccgtg
     1501 ctccgcgtgc tccaggcaga gcagcgaccc gtccgccgag agcccgcccc ggttgtagcc
     1561 gccctgtcgg ccggccacgc gcatgtgctc ggtgctgcgt gcgacctcct tgaccgtggc
     1621 accgccgtcc tcgctgacgt acaccgcgaa ggcgtcgtga gcgctgatcg aggcagccag
     1681 gatcccgggg acccacgcga tcccctcgtt ccagccgcgg tcgagctctg ggaacaggag
     1741 ccggggatcg ccgcccccga acgcttgcgc gtaccagcgt ccggactcgt cacccgtctc
     1801 gtcctgccac cagcagatgt cggctccgtc cggcgaccag gcacccgaga ccacgcccac
     1861 cgggtgatcg gtgacccgcc ggcggacgcc gctgtcgaga tcggccacgt gcagctgcca
     1921 gaccccgctc tccgtggact cgtacgcgag ccgggacgga gcggcggggg accagtcggg
     1981 gtacgtgatg acgggcgcgc ggaatcgccg ttcccagggt ggcgtcatcg gcgcaacggt
     2041 agcagcgcgc ggatcagtcg agggccgcga tcaaccggac gaagagggcg gtccctccgg
     2101 ccatcgcacc gatcagcccc gcccgcgcga cgaggagcgc cccccatcgc tccgggtgga
     2161 tccggagcgt ggatgccgtg cagatcgcgg ccgccgtcgc gaggacgacc ccccacccga
     2221 tccccgtcca cagactgatc ggatcgagga tctcgaacgt cttc
//
LOCUS       DASUVE010000070         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1351453, whole genome shotgun sequence.
ACCESSION   DASUVE010000070 DASUVE010000000
VERSION     DASUVE010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351453"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VF036_01435"
     CDS             <1..1015
                     /locus_tag="VF036_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263924.1"
                     /translation="RRALEVAEDEGRAHLDELLTATQQRLAGQSEKLIAAEERVREME
                     RQAALDAERLEELQAKLRQHQMTDQMRELQGPKHEPRETEDAAPAEDRRLSTPFLKEM
                     SMDAQKTISRMIGVTQLLKHKPGAKEQTQLVQQLTAHARRLDNTIRDLSDIDSLVTGT
                     IALEWRNTDLESLMRRVVDESGIETDQDVRVEAEPVTISVDRTRTEQIILGLLGGVAD
                     RTPTGKTITMRLLHERGGALIVVEDAEASSETSLSPMVRRLAEVQGGWAKIEPRDGGG
                     SSFTVFLPAGGAAGPEASEPAEDRSAPEPVVDEVEHDPNHWVKAEQALVRELRQLSQG
                     KAK"
     gene            complement(1019..2206)
                     /locus_tag="VF036_01440"
     CDS             complement(1019..2206)
                     /locus_tag="VF036_01440"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017570779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX6263925.1"
                     /translation="MPSTRSVIVAGARTPTGKFRGAFSELRAVELGAHAIRAAVERAG
                     IAGEQVDYTILGHVLQAGAGQITSLQASIGGGLPKDVPAITVNKVCLSGMSAIAMADQ
                     MIRGGEIEVAVAGGMESMTNAPFVLPKGREGVRLGDTELIDSMIHDGLWSSFLDHHMG
                     EGADEVGAELGLGREEQDAWAARSHRRAHAAWESGRMAEEVVPVEVPQRRGAPVSVDR
                     DEGIRPDTSPESLAGLRPAFRTDGTITAGNASQISDGASAVVVMSEERAEELGVTPIA
                     EIVAYGMSSDRYPSLQTVPAMALERALKKSGLAASDLGLVEINEAFASVPIHSARLLG
                     VDDEIVNVNGGAIALGHPIGSSGARIVLTLVLEMRRRGVDLGGAAICGGGGQGDALVL
                     RTA"
BASE COUNT          377 a          817 c          740 g          293 t
ORIGIN      
        1 gaggcgagcg ctggaggtcg ccgaggacga aggtcgcgca cacctcgacg agctgttgac
       61 ggcgacccaa cagcggctcg ccggacagag cgagaagctg atcgccgcgg aagaacgggt
      121 ccgcgagatg gagcggcagg cagcgctcga cgccgaacgg ctcgaggagc tccaggcgaa
      181 gctccggcag caccagatga ccgatcagat gcgcgagctc cagggcccga agcacgagcc
      241 tcgggagacc gaggacgccg cgcccgcgga ggatcgtcgg ctgtcgacac ccttcctgaa
      301 ggagatgtcg atggacgcgc agaagacgat ctcccggatg atcggggtga cgcagctcct
      361 caagcacaag cccggcgcca aggaacagac acagctcgtc caacagctca ccgcacacgc
      421 tcgccggctc gacaacacga tccgtgacct gtccgacatc gactccctcg tgaccggcac
      481 gatcgcgctc gagtggcgga acaccgacct cgaatcgctg atgcgccgcg tcgtcgacga
      541 atcggggatc gagacggacc aggacgtccg ggtggaggcg gagcccgtca ccatctcggt
      601 cgaccgcacg aggaccgagc agatcatcct cggcctgctc gggggcgtcg ccgaccgcac
      661 gcccacgggc aagacgatca cgatgcgcct cctccacgag cggggcggag ccttgatcgt
      721 cgtcgaagac gcggaggcct catccgagac gtctctgagc cccatggtgc gccggctcgc
      781 ggaggtgcag ggcgggtggg ccaagatcga gccccgcgac ggcggcggtt cctcgttcac
      841 ggtcttcctc cccgcgggcg gggcggccgg cccggaagcg tcggagcccg cggaggaccg
      901 atccgcgccc gagcccgtcg tggacgaagt cgagcacgac cccaaccact gggtgaaggc
      961 cgagcaggca ctcgtccgcg agctgcgtca gctctcgcag ggcaaggcga agtagccgct
     1021 acgccgtccg caggacgagc gcgtctccct gtccaccgcc gccgcagatc gcggcgcctc
     1081 cgaggtcgac accgcgccgc cgcatctcca atacgagggt gagcacgatc ctcgcgcccg
     1141 aggagccgat cggatgtccg agcgcgatcg ctcccccgtt cacgttcacg atctcgtcgt
     1201 cgacgccgag cagccgggcg gagtggatcg gcaccgaggc gaacgcctcg ttgatctcga
     1261 cgagcccgag gtccgaggca gccagacccg acttcttcag cgcgcgctcc agcgccatcg
     1321 ccgggaccgt ctgcagggac ggatagcgat cggacgacat cccgtaggcg acgatctcgg
     1381 cgatcggcgt cacccccagc tcttccgcgc gctcttcgct catcaccacg accgcgctgg
     1441 cgccatcgga gatctgggag gcgttccccg cggtgatcgt gccgtcggtc cggaacgccg
     1501 gccggagtcc ggcgagcgac tccggcgacg tgtcgggacg gatcccctcg tcgcgatcga
     1561 ccgacaccgg ggctccccga cgctgcggga cctctacggg aacgacctcc tccgccatcc
     1621 ggccggactc ccatgccgcg tgcgcgcgcc ggtgcgaccg tgccgcccac gcatcctgct
     1681 cctcccggcc gaggccgagc tccgctccca cctcgtccgc gccctcaccc atgtggtgat
     1741 cgaggaacga cgaccagagg ccgtcgtgga tcatcgagtc gatcaactcc gtgtcaccga
     1801 gtcgcacgcc ctcccgaccc ttcgggagga cgaacggggc gttcgtcatc gactccatcc
     1861 cgccggcgac cgccacctcg atctcgcccc cccggatcat ctgatcggcc atcgcgatcg
     1921 ccgacatccc ggacagacag accttgttga ccgtgatcgc agggacgtcc ttgggaagtc
     1981 cgcctccgat cgacgcctgg agcgacgtga tctggccggc tcccgcctgc aggacgtgac
     2041 ccaggatcgt gtagtcgacc tgctcgccgg cgatgccggc gcgctcgacg gccgcacgga
     2101 tcgcgtgcgc cccgagctcg accgcccgga gctccgagaa cgcgccgcgg aacttcccgg
     2161 tcggcgtcct cgcgcccgcg acgatcacgg atcgggtgga cgggatcacg gatcgagtct
     2221 acggcgg
//
LOCUS       DASUVE010000071         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_599201, whole genome shotgun sequence.
ACCESSION   DASUVE010000071 DASUVE010000000
VERSION     DASUVE010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599201"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /gene="sufB"
                     /locus_tag="VF036_01445"
     CDS             complement(<1..1239)
                     /gene="sufB"
                     /locus_tag="VF036_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX6263926.1"
                     /translation="MAVSQEEHLKELSKGYRFDWKDPSHSVFEPKRGLSTQVVEEISE
                     LKSEPDWMRKYRLKALKHFQSRPMPWWGADLSDIDFENIYYFIRSTEKQAQNWEDLPE
                     DIRGTWDKLGIPEAEKKYLGGVSAQYESEVVYHKIKKELDDIGVLFTDMDSALRDYPD
                     LVKEYFGTIIPPNDNKFAALNTAVWSGGSFIYVPPGVAVDIPLQAYFRINAENMGQFE
                     RTLIIADEGSYVHYVEGCSAPIYTSDSLHSAVVEIVAKKGARVRYTTIQNWSTNVYNL
                     VTKRAAAYEDSVMEWIDGNIGSKVTMKYPSIYLLGKGARGEVLSVAFAGKGMHTDAGG
                     KAIHAAPYTTSVINSKSVSKDGGRTGYRGLVRVEPDAHHAKSTVRCDALILDDESRSD
                     TYPYIEVEDETAALGHEATVS"
     gene            complement(1334..2497)
                     /locus_tag="VF036_01450"
     CDS             complement(1334..2497)
                     /locus_tag="VF036_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX6263927.1"
                     /translation="MTLLELGVVILGLAILARVAAKFDLPTVPLYLLAGLAFGEGGIL
                     PLVTTRVFVETGAEIGLILVLFVLGLEHSSRDLLATARSAVTPGVVDLLLNAAPGVIA
                     GLVLGWGWLAAAFLGGITYVSSSGVAAKLLEHAGPHARGAGRFVVTIAVIEDLVMAIY
                     LPILAAVLIGASAGVSLVAAAVAIGAVALLLVVALRVEVGLSQLLFSRSDEALLLSIL
                     GFAFLIAGVAEVARFSAAVGALLAGIMLSGPAAAGAQTLVRPLRDLFAAIFFAFIGLS
                     IDPSTIPPILWIAVLLGVVTVATKFATGWWAAGRMGSEPAARGVVGAALVPRGEFSIA
                     IAGLATAAAVEDDLGPVTVAYVIFTVVVGSAITKILARRVDRWDPADVAERHG"
BASE COUNT          448 a          891 c          828 g          414 t
ORIGIN      
        1 cgagaccgtg gcctcgtggc cgagcgcggc cgtttcgtcc tcgacctcga tgtacgggta
       61 cgtgtccgac ctcgactcgt cgtcaaggat cagcgcgtcg caacgcaccg tggacttcgc
      121 gtgatgggcg tcgggctcca ctcggacgag gcctcggtac ccggtgcgac ccccgtcttt
      181 cgagacggac ttcgagttga tcacgctggt cgtatacggc gccgcgtgga tcgctttgcc
      241 gccggcgtcg gtgtgcatac ccttgccggc gaacgctacg gacagcacct cgccgcgcgc
      301 gcccttgccg agcaggtaga tcgacgggta cttcatcgtg accttcgagc cgatgttgcc
      361 gtcgatccac tccatcacgg agtcctcgta ggcagccgcg cgcttggtga ccaggttgta
      421 cacgttggtc gaccaattct ggatcgtcgt gtagcggacg cgggcgccct tcttcgcgac
      481 gatctccacg accgccgagt gcagtgagtc gctggtgtag atcggcgccg agcagccctc
      541 gacgtagtgc acgtaggaac cctcgtcggc gatgatcagc gtccgctcga actgccccat
      601 gttctcggcg ttgatccgga agtaggcctg cagcgggata tccacggcca caccgggagg
      661 cacgtagatg aacgagccgc ccgaccagac cgccgtgttc agcgcagcga acttgttgtc
      721 gttgggcggg atgatcgtcc cgaagtactc cttcacgagg tccgggtagt cgcggagcgc
      781 ggaatccatg tccgtgaaca ggacgccgat gtcgtccagc tccttcttga tcttgtggta
      841 cacgacctcg gactcatact gcgcgctcac gccaccgagg tacttcttct ccgcctcggg
      901 gatcccgagc ttgtcccagg tgccgcggat gtcctcgggg aggtcctccc aattctgggc
      961 ctgcttctcg gtggaccgga tgaagtagta gatgttctcg aagtcgatat cggagaggtc
     1021 cgcgccccac cagggcatcg ggcggctctg gaagtgcttc agcgctttca gccggtactt
     1081 gcgcatccag tcgggctcgc tcttgagctc gctgatctcc tcgacgacct gcgtcgagag
     1141 accccgcttg ggctcgaaga ccgagtggga cgggtccttc cagtcgaagc ggtatccctt
     1201 ggaaagctcc ttgagatgct cttcctggct gacggccatg tcactccctc gacgctcgcc
     1261 ggtgacggtc ccctacaggc tacccgcggt actgctctga agtctatctc ttaggtcgga
     1321 ttcatcccgg gggtcacccg tggcgctccg cgacgtcggc cggatcccac cggtccaccc
     1381 ggcgggccaa gatcttcgtg atcgccgagc cgacgacgac ggtgaagatc acgtaggcga
     1441 ccgtgaccgg gccgaggtca tcctcgacgg ctgcggccgt ggcgagccct gcgatcgcga
     1501 tcgagaactc cccacgcggg accagcgcgg ctccgaccac tcctcgggcc gccggttccg
     1561 accccatccg tccggccgcc caccagccgg tggcgaactt cgtagcgacc gtcacgactc
     1621 cgaggagcac cgcgatccac aggatcggcg ggatcgtgct cggatcgatc gagagaccga
     1681 tgaacgcgaa gaagatcgcg gcgaacaggt cgcggagcgg ccgcacgagc gtctgcgcgc
     1741 cggcggccgc gggacccgac agcatgatgc ccgccagcag cgctccgacc gctgccgaga
     1801 atctcgccac ctcggcgacg ccggcgatca ggaaggcgaa tccgaggatc gacaacagga
     1861 gtgcctcgtc ggatcggctg aagagcagct ggctcaggcc gacttccacc cggagtgcca
     1921 ccacgagcag gagcgcgacc gcgccgatcg ccacggcggc cgcgacgagg gacacgccgg
     1981 cactcgcgcc gatcaggacg gccgccagga tcgggagata gatcgccatc acgaggtcct
     2041 cgatcaccgc gatcgtcacg acgaatcggc cggcgccccg agcgtgcggc cctgcatgct
     2101 ccaagagctt cgcggcaacg ccggaggaag aaacgtacgt gatccccccg aggaacgccg
     2161 cggccagcca cccccacccc agcacgagcc ccgcgatcac tcccggcgcc gcattcagga
     2221 gcaggtcgac cacgccgggc gtcacggcgc ttctggcggt ggcgagcagg tcccgtgacg
     2281 agtgctcgag cccgagcacg aacaggacga ggatcagccc gatctccgca ccggtctcga
     2341 cgaacacccg tgtcgtgacg agcggcaaga tcccaccctc gccgaacgcg agccccgcga
     2401 gcaggtacag aggaacggtc ggaagatcga acttggccgc gacacgcgcg aggatcgcca
     2461 ggccgaggat cacgacgccc agctcgagga gcgtcaccag cggcgaccag gcatcgtggc
     2521 cctcatcgcc tcgagtctat gagtggccct cgacctccga ccccgagcca ctcaggagcg
     2581 g
//
LOCUS       DASUVE010000072         2958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_787089, whole genome shotgun sequence.
ACCESSION   DASUVE010000072 DASUVE010000000
VERSION     DASUVE010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787089"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1304
                     /locus_tag="VF036_01455"
     CDS             147..1304
                     /locus_tag="VF036_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEX6263928.1"
                     /translation="MGTDELVVIDLPPGPGWVELVNRLWLEEVPFLPLDHRLTEAERR
                     AIVDRARPHFVQERGGETLLADALPVTPGVAIVMPTSGTAGEPKLVELHRVAVVVAVS
                     RSAKRLGATPGAPWVCPLTPAHIGGLLVLLRGAILGAPVTMHERFDPERLVRDGDGAA
                     FASVVPAMVRRLVASDLGLHGLTLVVGGDGIDDETAARARSRGATVVTTYGLTETCGG
                     FTYDGVPLDGMQVRTDAAGAIEASGSTLMEGYRLDPAATGAAFTTDGWLRTGDVGAID
                     DDGRLSIHGRADHVIRTGAEKVWPEEVERALSSHPKVRDVAVAGSPDPDWGSHVRALV
                     VPARPEDPPSLEELRGHGAERLARFKLPRELRLVEEIPRTSTGKVRRGALP"
     gene            complement(1565..2221)
                     /locus_tag="VF036_01460"
     CDS             complement(1565..2221)
                     /locus_tag="VF036_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6263929.1"
                     /translation="MTTLLICDDHKVLTDALSTVVGLDEDLQLLAAPVHDPETAIELS
                     AEHLPDVVLMDIVFKGGMSGIEATRRIKEISPATKVVIMTAHDEDRLLVEAVEAGASG
                     FLGKGEAVEEVLAAAKAAAEGEVLIDPATLTRLLAQVAREREAHREASMLLDDLTDRE
                     REILQLLAEGMRNEGIATKLFISPQTVQTHVRNILGKLRVHSKLEAVAFAVKHGAIVV
                     "
     gene            complement(2218..>2958)
                     /locus_tag="VF036_01465"
     CDS             complement(2218..>2958)
                     /locus_tag="VF036_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6263930.1"
                     /translation="LPRLAGVTGDDGREDTLAERAALVSKLTHEIRGPVSTIRGLAST
                     TLTHYDRLDDGERREFLELIRHEAERLEHTVEQVALALKLDAGTLRFDIRMQDLGVIV
                     RGAADAAETGDHPLEVQTDEQVEAPVDGIQVAFVVRQLLENAAIFSPTGSPIALRMTS
                     ETDHAVIHVIDRGPGIPAGEREGVFERFAAWRPTGYEDRPGSGLGLFICRAIVEEHGG
                     EASIVDGPTGGTMLVVRLPLEARMAGSR"
BASE COUNT          413 a         1024 c         1066 g          455 t
ORIGIN      
        1 gtcgggagcg gccgatcagg tccaccccgt gtccaatcca aggccggtcg gatctcacgt
       61 gactcgtcca tatgaccgct gactcctaga gcgcacgctc gaacggttgg gtagcctacc
      121 cgacgcgccg agggcgaggg aacgtcgtgg gaacagacga actggtggtg atcgacctgc
      181 cgcccggtcc cggatgggtc gagctcgtca accgactctg gctcgaggag gtgccgttcc
      241 tcccgctcga ccaccggctc accgaagcgg agcggcgggc gatcgtggac cgcgctcgtc
      301 cgcacttcgt gcaggagcgt ggtggcgaga cgctcctggc cgacgcccta ccggtcacgc
      361 cgggtgtcgc gatcgtgatg ccgacgtcgg ggacggcggg ggagccgaag ctggtggagc
      421 tccaccgcgt ggccgtcgtc gtcgccgtga gtcggtcggc gaagcggctg ggcgccaccc
      481 cgggtgctcc gtgggtctgc ccgctcaccc ccgcgcacat cggcgggctg ctggtgctcc
      541 tccgcggcgc catcctcggc gctccggtga cgatgcacga gcggttcgat cccgagcgcc
      601 tcgtccgtga cggcgacggc gccgcgttcg cgtcggtcgt cccagcgatg gtccgccgcc
      661 tcgtggcgtc ggacctcggt ctgcacggcc tgacgctcgt cgtggggggc gacggcatcg
      721 acgacgagac agccgctcga gcgcgttccc gcggcgccac cgtcgtcacg acgtacggcc
      781 tgacggagac gtgcggaggg ttcacgtacg acggcgtgcc cctcgacggg atgcaggtcc
      841 gcacggacgc cgccggcgcg atcgaggcct ccgggtccac gctgatggag ggatatcgac
      901 tcgaccccgc cgccacgggc gcggccttca ccaccgacgg ttggctccgc acgggcgacg
      961 tcggggcgat cgacgacgac ggccgtctct cgatccacgg gagggcggac cacgtgatcc
     1021 ggaccggcgc ggagaaggtc tggcccgagg aggtcgagcg ggcgttgtcg tcccacccga
     1081 aggtgcgcga cgtggccgtg gccgggagcc ccgatcccga ctggggatcg catgtacgag
     1141 cgctcgtggt gccggctcgt ccggaggatc cgccgtcgct cgaggagttg cgcggccatg
     1201 gcgccgagcg actggcgcgg ttcaagctcc cgcgcgagct gcggctggtg gaggagatcc
     1261 ctcggacctc gaccgggaag gtccgccgcg gcgcactgcc ctgatggcgc cgtcgggttc
     1321 gcgccgtcgg gttcgcgccg tcgggttcgg cgccgtgggg atccgaagtg ccaagaacga
     1381 acgatgaaag gcccgagtcc gggaggggtg gactcgggcc ttgctcgggg aggtccccga
     1441 ggccatcgag ggggtgacgg cctctccgcg cccagggcgc gggtcgctct ccggtactgg
     1501 tttcgtcctc cccaatacgt gacttgagcc cagccgggca ggagtttccc gacctcgaac
     1561 gggctcagac gacgatcgcg ccgtgcttca cggcgaaggc gaccgcctcg agcttggagt
     1621 gcactcggag cttgccgagg atgttccgga cgtgcgtctg gaccgtctgg gggctgatga
     1681 acagcttcgt ggcgatgccc tcgttccgca tgccctcggc gaggagctgg aggatctccc
     1741 gctcacggtc ggtgagatcg tcgagcagca tggaggcctc ccggtgggcc tcacgctcgc
     1801 gagcgacctg agcgagcagc cgtgtcagcg tcgcgggatc gatcaggacc tcaccctcgg
     1861 ccgcggcctt cgcggcggcg aggacctcct cgacggcctc gcccttgccg aggaacccgg
     1921 aggcaccagc ctcgaccgcc tccaccagca agcgatcctc gtcgtgcgcc gtcatgatca
     1981 cgaccttcgt ggcgggcgag atctccttga tccggcgcgt cgcctcgatg ccggacatcc
     2041 ctcccttgaa gacgatgtcc atcaacacca cgtcgggcag atgctccgcg gagagctcga
     2101 tcgccgtctc ggggtcgtga acgggtgcgg ccaggagctg caggtcctcg tcgaggccga
     2161 cgacggtcga cagggcgtcc gtcaggacct tgtggtcgtc gcagatcagg agcgtggtca
     2221 ccgggagccc gccattcgtg cctccagggg aagccggacg acgagcatcg taccccccgt
     2281 gggaccgtcc acgatcgaag cctcgccccc gtgttcctcg acgatcgccc ggcagatgaa
     2341 cagcccgagg cccgatccgg gtcgatcctc gtagccggtc ggccgccatg cggcgaaccg
     2401 ctcgaagacg ccctcccgtt cgcccgccgg gatcccgggc ccgcggtcga tcacgtggat
     2461 caccgcgtgg tcggtctcgg acgtcatgcg gagcgcgatc ggcgaaccgg tcggcgagaa
     2521 gatcgcggcg ttctcgagga gctggcggac cacgaacgcg acctggatgc cgtcgaccgg
     2581 tgcctcgacc tgctcgtccg tctgcacctc gagaggatgg tcaccggtct ccgcggcgtc
     2641 cgcggcaccg cgcacgatca cgccgagatc ctgcatgcgg atgtcgaatc ggagggtccc
     2701 ggcgtcgagc ttcagcgcga gggccacctg ctcgacggtg tgctcgagcc gttccgcctc
     2761 gtgacggatc agctcgagga actcgcgccg ctcgccgtca tcgagccggt cgtagtgggt
     2821 gagggtcgtg ctggccagcc ctcggatcgt ggagaccggc ccccggatct cgtgcgtgag
     2881 cttcgagacg agagcggccc gctccgcgag ggtgtcctcc cgcccgtcgt cgccggtcac
     2941 gcccgcgagt ctagggag
//
LOCUS       DASUVE010000073         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_787239, whole genome shotgun sequence.
ACCESSION   DASUVE010000073 DASUVE010000000
VERSION     DASUVE010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787239"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /gene="folE"
                     /locus_tag="VF036_01470"
     CDS             complement(<1..410)
                     /gene="folE"
                     /locus_tag="VF036_01470"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEX6263931.1"
                     /translation="MDLLGPPDSFDAKKVEEGVRLILEGIGEDPDRGGLRETPARVAR
                     MYEETFAGIGKDASELVTVVEGADHDEMIMVRDIPLQSFCEHHLIPFAGKAHVAYIPN
                     KAQQITGLSKIARVVDLLSKKPQVQERLTTEIAEA"
     gene            complement(421..1083)
                     /locus_tag="VF036_01475"
     CDS             complement(421..1083)
                     /locus_tag="VF036_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6263932.1"
                     /translation="MTDRWATFDCYGTLIDWDGGVRRALVRVWPDEAGDRLFQRYHDV
                     EPRIQAGRGITYRQVLRETLVTIAEEDELEVPPGQDDALADSVPSWRPFPEVPGALGE
                     LRDRGWKLAILSNTDPDMLAASMKTIGVEVDLRITAAEAGSYKPAYGHWDTFFRTTSA
                     DRARHVHVAASLYHDVEPCAQLGLPCVWINRLDESSELPRAGELRNLAGLPAALDALV
                     PR"
     gene            complement(1097..>2078)
                     /locus_tag="VF036_01480"
     CDS             complement(1097..>2078)
                     /locus_tag="VF036_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEX6263933.1"
                     /translation="LDPALLRPGRFDRQIVVDRPDLEGRKAILHIHAKGKPLDPGVDL
                     DILARRTPGFTGADLANVINEAALLAARRSKKTITMREVEEAVDRVMAGPERKSRVMS
                     EQERRLIAYHEGGHAMVAHVLPNTDEVHKITVIPRGRALGYTLTLPEQDRFMMTREEL
                     RDELAMLMGGRVAEEIIAGDISTGAGNDIERATKIARQMVTEHGMSDAIGPRTLGQKQ
                     GEVFLGRDWASTPDYSEAVAFEIDQEISRLIDEAHDVALEILSQHRERLDELAALLLQ
                     KETLDRDEVEAFFKAVEKRAPRAPEVRSAGLAVSRTEMFPPPDAPGPLRR"
BASE COUNT          287 a          733 c          698 g          360 t
ORIGIN      
        1 gcctccgcga tctcggtcgt caggcgttcc tggacctgcg gcttcttcga gagcaggtcg
       61 acgacccgcg cgatcttcga cagaccggtg atctgctgcg ccttgttcgg gatgtacgcg
      121 acgtgcgcct tcccggcgaa cgggatcagg tggtgctcgc agaacgattg cagcgggatg
      181 tcgcggacca tgatcatctc gtcgtggtcg gcgccttcca ccaccgtgac gagctccgac
      241 gcgtccttcc cgatcccggc gaacgtctcc tcgtacatgc gtgccacgcg ggcgggcgtt
      301 tcccgcagtc caccgcggtc ggggtcctcg ccgatacctt cgaggatcag gcgtacgcct
      361 tcctcgacct tctttgcgtc gaacgagtcg ggtggcccga gcagatccat cgcgcggatg
      421 ctaccgggga accaacgcgt ccagggcggc ggggaggccg gcgaggttgc gaagctcgcc
      481 ggcgcgtggg agctcgctcg actcgtccag cctgttgatc cagacgcacg ggagcccgag
      541 ctgcgcacac ggctcgacat cgtgatacag cgatgccgcc acgtggacgt ggcgggcacg
      601 gtcggccgag gtcgttcgga agaacgtgtc ccaatgaccg tacgcgggct tgtacgagcc
      661 ggcctctgcc gccgtgatcc gcagatccac ctccaccccg atcgtcttca tcgaggcggc
      721 gagcatgtcc gggtcggtgt tcgacaggat cgcgagcttc cagccacgat cgcggagctc
      781 gccgagcgct cccggcacct cggggaacgg tcgccacgac gggacggagt cggcgagcgc
      841 gtcgtcctgt cccggaggga cctcgagctc atcctcctcg gcgatcgtga cgagcgtctc
      901 acgcaggacc tgccgatagg tgatgccccg cccggcctgg atgcgcggct ccacatcgtg
      961 gtagcgctgg aacagccgat cgccggcctc gtccggccag accctgacca gcgcccggcg
     1021 cacgcctccg tcccagtcga tcagcgtgcc gtagcagtcg aacgtcgccc agcggtccgt
     1081 catcgcagac gttccctcac cgacggagcg gtcctggcgc gtcgggaggg gggaacatct
     1141 cggtccggga caccgcgagg ccggcgctgc ggacctccgg agcgcgggga gcgcgcttct
     1201 cgacggcctt gaagaacgcc tccacctcgt cgcgatccag tgtctccttc tgcagcagga
     1261 gggccgcgag ctcgtcgagc cgctcccggt gctggctcaa gatctcgagc gccacgtcgt
     1321 gcgcctcgtc gatcagccgg ctgatctcct ggtcgatctc gaacgcgacg gcctccgagt
     1381 agtcgggcgt ggacgcccag tcgcggccga ggaagacctc gccctgcttc tgcccgagcg
     1441 tcctcggccc gatcgcgtca ctcatcccgt gctccgtcac catctggcgg gcgatcttcg
     1501 tcgcgcgctc gatgtcgttg cccgcgccgg tcgagatgtc gccggcgatg atctcctccg
     1561 cgacccgtcc gcccatcagc atcgcgagct cgtcgcggag ttcctcacgc gtcatcatga
     1621 acctgtcctg ctcggggagg gtgagcgtgt acccgagggc gcggccgcgg gggatcacgg
     1681 tgatcttgtg gacctcgtcg gtgttcggga ggacgtgcgc gaccatcgcg tgaccccctt
     1741 cgtggtaggc gatcagccgc cgctcctgct cgctcatcac gcggctcttg cgctccggtc
     1801 cggccatcac ccggtcgacg gcctcctcga cctcccgcat cgtgatcgtc ttcttggacc
     1861 gtcgcgccgc gaggagcgcg gcctcgttga tcacgttcgc gagatcggcg cccgtgaagc
     1921 ccggggtgcg ccgcgcgagg atgtcgaggt cgacccccgg atcgagcggc ttgcccttcg
     1981 cgtggatgtg gaggatcgct ttcctgccct cgaggtcggg ccggtcgacg acgatctgtc
     2041 ggtcgaaacg ccccggccga agcagggccg gatcaagg
//
LOCUS       DASUVE010000074         2917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_223427, whole genome shotgun sequence.
ACCESSION   DASUVE010000074 DASUVE010000000
VERSION     DASUVE010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223427"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /gene="uvrC"
                     /locus_tag="VF036_01485"
     CDS             <1..1284
                     /gene="uvrC"
                     /locus_tag="VF036_01485"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX6263934.1"
                     /translation="DFLAGNSKPVLRRLEEEMAEASGREEYEQAARFRDQLQAARRAL
                     ESQEMVLVQPESLDVVGLAEDDLEAAFQVFTVRGGRVLGRKGWIVDRVEDLDRPELVA
                     SFLRQLYMEREEVPPRILVPTEPADREVLETWLSTRRGSHVTIAVPARGAKRRLMEVV
                     GNNASEAFHRHKLRRASDFGARSRALSELADQLGLEQAPLRIECYDISNLGPSDTVGS
                     MVVFEDGLPKRADYRRFEIKGVPGQDDFASMEEMLTRRFSRLLKEKAEPPGGRRRFSY
                     PPALVVVDGGRGQLSVATRVLEDLGLHIPHIGLAKRLEEIYFPDRPDPLSIARGSEAL
                     FVLQHIRDEAHRFAITYHRQRRDRRALSSPLDEIPGVGPSRKRALMKRFGSLTKIRQA
                     GAADLAATPGVGPRLADEIHRRLHAVSVTDRRESA"
     gene            1296..2180
                     /gene="rapZ"
                     /locus_tag="VF036_01490"
     CDS             1296..2180
                     /gene="rapZ"
                     /locus_tag="VF036_01490"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX6263935.1"
                     /translation="MRRSARETAAPGFLLITGLSGAGRSEAARSLEDLGFFVVDNLPP
                     ALLPKMAELAASPGGPRRVAIVVDARGGVFFGELSKALEELREQRVPSRIVFLDASDE
                     DLVNRYEATRRRHPLAPGDRVGEGIRKERLMMESLRADADLVIDTTGLTPHDLRDRMR
                     ETFADTPPEAGLLVSIVSFGFKYGSPRDADLVLDVRFLPNPHWVEELRPLPGSNEKVR
                     EYVEGQEEYGSFMKRLGGLLDVVVPGYVDEGKSYLTIAIGCTGGRHRSVVAADDLADW
                     FREHGHRVSVNHRDVDRD"
     gene            2329..>2917
                     /locus_tag="VF036_01495"
     CDS             2329..>2917
                     /locus_tag="VF036_01495"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX6263936.1"
                     /translation="MSVKVGINGFGRIGRNLLRAVRDTGADVDFVGVNDITDAKTLAH
                     LLKYDSVLGRLDAEVAVSDNGLSVDGDELRVFAERDPAQLPWKEVGAEIVIESTGLFT
                     DREKAAAHLEAGAQKVIISAPAKGEDITIVMGVNDDVYDPTSHHVISNASCTTNCVVP
                     LAKVLEDSFGIDRALMTTVHAYTNDQNLLDLPHKDL"
BASE COUNT          474 a          996 c         1022 g          425 t
ORIGIN      
        1 gacttcctcg cggggaactc gaagccggtg ctcaggcgcc tcgaagagga gatggctgag
       61 gcgtcgggtc gggaggagta cgagcaggcg gcgcggttcc gcgaccagct ccaggcggcg
      121 cgccgggcgc tggagagcca ggagatggtc ctcgtccagc ccgagagcct ggacgtcgtg
      181 ggcctcgccg aggacgacct cgaggccgcc ttccaggtct tcacggttcg cggcggacgg
      241 gtcctcggca ggaagggatg gatcgtcgat cgcgtggagg acctcgaccg ccccgagctc
      301 gtcgcgtcgt tcctccggca gctctatatg gagcgcgagg aggtaccgcc gcggatcctg
      361 gtgccgacag agccggccga ccgagaggtg ttggagacct ggctctcgac ccgtcgcggt
      421 tcccacgtca cgatcgccgt gccggcgcgc ggcgccaagc ggcgcctgat ggaggtcgtg
      481 gggaacaacg cgagcgaagc gttccatcgg cacaagctcc gacgtgcctc ggacttcggg
      541 gcgcggtcca gggcgctgtc cgagctcgcc gatcagctcg gcctcgagca agcacccctc
      601 cgcatcgagt gctacgacat ctcgaacctc gggccgagcg acacggtggg atccatggtg
      661 gtcttcgagg acggcctccc gaagcgagcc gactaccgcc ggttcgagat caagggcgtg
      721 cccggacagg acgatttcgc gagcatggag gagatgctca cccgccggtt ctcacgactg
      781 ctgaaagaga aggcggagcc accgggcggc cggcgacgct tctcgtaccc accggcactc
      841 gtggtggtgg acggcgggcg tggtcagctg agcgtcgcga cgcgggtcct cgaggacctc
      901 ggcctgcaca tcccgcacat cgggctcgcg aagcgactcg aggagatcta cttccccgac
      961 cgcccggatc cgctgtcgat cgcgcgcgga tcggaggcgc tcttcgtcct ccagcacatc
     1021 cgtgacgagg cacaccgctt cgcgatcacg tatcaccgtc aacggcgcga ccgtcgggcc
     1081 ctctcctctc cgctcgacga gatccccggc gtgggaccgt cccggaagcg cgccctgatg
     1141 aagcgcttcg gctccctgac gaagatccgt caggcgggag cggccgacct cgccgccaca
     1201 ccgggcgtcg ggccgcggct cgccgacgag atccatcgcc ggctgcacgc ggtgtcggtc
     1261 acggatcgtc gggagagcgc atgatggcac gggctgtgag gcgatcggcg cgggagaccg
     1321 ccgctcccgg gttcctgttg atcaccgggc tgtcgggggc cgggcgctcc gaggccgcgc
     1381 ggagcctcga ggacctcggg ttcttcgtcg tcgacaacct gccgccggcg ctcctgccga
     1441 agatggccga gctggccgcg agcccgggcg gtccccgacg ggtggcgatc gtcgtggacg
     1501 cgcggggcgg cgtgttcttc ggcgagctgt cgaaggcgct cgaggagctg cgggagcaac
     1561 gggtcccctc caggatcgtc ttcctcgacg cctcggacga ggaccttgtc aaccggtacg
     1621 aggcgacccg ccggcgccac ccgctggctc cgggcgaccg ggtgggggag gggatccgca
     1681 aggagcgact gatgatggag agcctccgcg ccgacgcgga cctcgtgatc gacaccacgg
     1741 gactcacgcc gcacgacctc cgcgaccgga tgcgagagac gttcgccgac acaccgcccg
     1801 aggccggcct gctcgtctcc atcgtatcgt tcggcttcaa gtacgggagc ccgcgcgacg
     1861 ccgacctcgt gctggacgtg cggttcctcc ccaacccgca ctgggtcgag gagctccgcc
     1921 cccttccggg aagtaacgag aaggtgcgcg agtacgtcga gggacaggag gagtacgggt
     1981 ccttcatgaa gcggctcggg ggcctcctgg acgtcgtcgt ccccggctac gtggacgagg
     2041 ggaagtcgta cctgacgatc gcgatcggct gcaccggggg ccgacaccgc tcggtcgtgg
     2101 ccgcggacga cctcgccgat tggttccgag agcacgggca tcgcgtctcg gtgaaccacc
     2161 gcgacgtcga ccgcgactga cgaggttctc ccgcagtgcg gacgtccggt tccgtgcgtc
     2221 cacgccgtcg cccatcggcg ccaggcccca cggacggaca gcccccgagg cccgcccgta
     2281 ggatggaggg accgaggcgc caacgaggcc cccaggaggt tctccacgat gagcgtgaag
     2341 gttggcatca acggtttcgg acggatcggc cgcaacctcc tccgcgccgt cagggacacg
     2401 ggcgcggacg tcgacttcgt cggcgtcaac gacatcacgg acgcgaagac cctggcgcat
     2461 ctgctgaagt acgactcggt cctgggtcgt ctggacgcag aggtcgccgt ctcggacaac
     2521 ggcctctccg tcgacggcga cgagctccgt gtgttcgcgg aacgggatcc cgcacaactc
     2581 ccgtggaagg aagtgggcgc cgagatcgtg atcgagtcga ccggtctgtt cacggaccgt
     2641 gagaaggcag ccgcacacct ggaagccggc gcccagaagg tcatcatcag cgcccccgcc
     2701 aagggtgagg acatcacgat cgtgatgggc gtgaacgacg atgtctacga ccccacctcg
     2761 catcacgtga tctcgaacgc gtcctgcacg acgaactgcg tcgtgcccct cgcgaaggtg
     2821 ctcgaggatt ccttcgggat cgaccgcgcc ctgatgacca cggtgcacgc gtacacgaac
     2881 gaccagaacc tgctcgacct accgcacaag gacctgc
//
LOCUS       DASUVE010000075         8200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_976507, whole genome shotgun sequence.
ACCESSION   DASUVE010000075 DASUVE010000000
VERSION     DASUVE010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976507"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VF036_01500"
     CDS             <1..259
                     /locus_tag="VF036_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX6263937.1"
                     /translation="LISMAMNGWGAAEPAWWLNLQAHPEAVVELAGGIRREVLGRAAV
                     GVEREQLWQRWREIDEKLDGFAARRGHETAVVILEPRQMAG"
     gene            382..1179
                     /locus_tag="VF036_01505"
     CDS             382..1179
                     /locus_tag="VF036_01505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEX6263938.1"
                     /translation="MSDERTRVLRQLAEGGCVAPEAEADSLLIAAREGVGPIEELVRR
                     RLRGEPLAWITGSVAFCGHQVRIDPGVFVPRPHTEALARRAIELLPDDGIAVDLCTGS
                     GAVAVALAAARPRATVVGTDVDPQAIACAGRNGVAVSEGDLDVPLPFTLRGRVDLMTA
                     VVPYVPTEELHLLPRDVLTHEPRRALDGGSGGTFVLVRAAGAADRWLRPGGTVLLELG
                     GDQAADMTAILTDLGFTDVRVHTDADGQDRAIEGRLGPLPPGAVGRG"
     gene            complement(1150..1797)
                     /locus_tag="VF036_01510"
     CDS             complement(1150..1797)
                     /locus_tag="VF036_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEX6263939.1"
                     /translation="MAFHGWKVEALEFFEGLEADNSKAYWQRHKDVYDTIVRAPMEEL
                     LAELEPEFGEGRIFRPYRDIRFSRDKSPYKTAIGATLGDGYVQLDARGLAVGSGRWEM
                     APDQLERYRESVARDPSGEDLTRIVSDARTQGLDVHGHDVLKTAPKGYPKDHPRIELL
                     RFKGLIAWQEWPAGAWLGTKRAKDRIVTFLERSKPLVDWLESNVGDSTPPDRARR"
     gene            complement(1830..2801)
                     /locus_tag="VF036_01515"
     CDS             complement(1830..2801)
                     /locus_tag="VF036_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6263940.1"
                     /translation="MRRSSRSLRLSAAMIALATAIGLLAPGAGATDRDPVIVAVGDIA
                     CQSLTQGQGMGACRSGDVADLIREIDPDRFLALGDLQYNNGKLSEFLRVWDVQFGDLR
                     EITMPAPGNHEYGTTDAAGYFAYFGAVANPPLGNYSFDLGDWHIVSLNSDICGDDPGC
                     GPGTPQGDWLRADLEANADATCTLAFMHHPRYDWRPFQKWIEDDATINFGGSETRPLI
                     PLWEVFADEGVDVVLVGHNHLYQRWAPQDAHGNAVAGGTIQFTVGTGGRQLYPFGRPP
                     MPENLEVTQNKAFGVLQMTLHEDSYDYGWVSVPGEPAFQDGGTVACT"
     gene            complement(2888..3196)
                     /locus_tag="VF036_01520"
     CDS             complement(2888..3196)
                     /locus_tag="VF036_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263941.1"
                     /translation="MDDCQLCAAEQVTAWLHEDDECWVAECLVCRTPMIVWRSHGLPE
                     SELETELLARLERIATDRYGSDGYWIDPERRRIPEHWHAHARPAGGFFDPASDLYGSF
                     "
     gene            complement(3189..3653)
                     /locus_tag="VF036_01525"
     CDS             complement(3189..3653)
                     /locus_tag="VF036_01525"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX6263942.1"
                     /translation="MRQIIAAIVTLTLLGACTDDTPEAPETIPGSTMTFGGTDAILTV
                     DLADDPQERRKGLMGIEELPADQGMAFVWPGPVDSTFWMKDTLVPLSVAFVDESGYVI
                     DVLDMQPCETDACPSYGIDEPFVLAIEANLGWFDEHGVGAGDRAELRRALDG"
     gene            3708..3783
                     /locus_tag="VF036_01530"
     tRNA            3708..3783
                     /locus_tag="VF036_01530"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3741..3743,aa:Thr,seq:ggt)
     gene            4313..5242
                     /locus_tag="VF036_01535"
     CDS             4313..5242
                     /locus_tag="VF036_01535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6263943.1"
                     /translation="MSGSSGSHFDQPLEGGSPNDTPTPDLSTEQLLGGTYATHDLVVV
                     AGLLRAAGWSEERIRREVYAPFIVVGPASWADSWHAVRYGPGPAVRQHEGQDVLCRSG
                     SPVLAPEDGTIEFDTSLLGGRSARLLLPGGGHYYFAHFSDWNGREFSNGDDVQRGDVI
                     GFCGDTGNATVAHVHFGWYGSDGVAIDPMRRLIRWLRESEADLPALLSEAGLGGAATV
                     SEMLGSAEPSFDVVLALLEGRLAESRFIGWRNASATGSHGPLDLTPLLFLALAALTRR
                     GTTLGSRRRRRPIGAPIWTVPPEWEDRRGPLLS"
     gene            5345..5416
                     /locus_tag="VF036_01540"
     tRNA            5345..5416
                     /locus_tag="VF036_01540"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5377..5379,aa:Met,seq:cat)
     gene            5456..6640
                     /gene="tuf"
                     /locus_tag="VF036_01545"
     CDS             5456..6640
                     /gene="tuf"
                     /locus_tag="VF036_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX6263944.1"
                     /translation="MGKKKFERTKPHVNIGTIGHIDHGKTTLTAAITRRQAEKGMADF
                     TPFDQIDKAPEERERGITIAIAHVEYETDNRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHVLLARQVGVPSIIVALNKCDMVDDPELLDLVELEVRELLSS
                     YEFPGDDVPVVQISALKALEGDETWNGKIDELLAACDSYIPEPTRDTEKPFLMPIEDV
                     FSITGRGTVVTGRVERGTLNKMSEIEIVGIKDTRKTTATDLEMFRKLLDEVQAGDNVG
                     ILLRGIDKDDVERGQVLAKPGSITPHTEFEAQVYILSKEEGGRHTPFFDGYRPQFYFR
                     TTDVTGQAHLPGGVEMVMPGDNVEMKVDMIAPIAMEEGLRFAIREGGRTVGAGRVTKV
                     VK"
     gene            6643..6810
                     /gene="rpmG"
                     /locus_tag="VF036_01550"
     CDS             6643..6810
                     /gene="rpmG"
                     /locus_tag="VF036_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX6263945.1"
                     /translation="MARKEGNRPKITLACSDCKRRNYNTVKNRVNDRDRIELKKYCRW
                     CRSHTLHRETR"
     gene            complement(6931..7671)
                     /locus_tag="VF036_01555"
     CDS             complement(6931..7671)
                     /locus_tag="VF036_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6263946.1"
                     /translation="MAARARPKDRKASGTAEGDIRVVIADDHRSFGEALQIALDNEDD
                     LKVIEVVTDGESAIESAQVQHPDVLLMDVGMPGVDGLEATRRIHREASNTKVILLSGH
                     DDDVVLARAVEAGARGFLRKTQAVSELAEAIRRANRGDPLHEVSEVELSLARFRMQRR
                     TDGELAQRVERLTPRELEILQRVAAGEASVDIANELGMSRHTLRTHIQNVLTKLGVHS
                     KTDAVVAAIRFGKVKTNEKLAETDVDAG"
     gene            7793..8179
                     /locus_tag="VF036_01560"
     CDS             7793..8179
                     /locus_tag="VF036_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6263947.1"
                     /translation="MNAAAAGKVYPEAPFTVDPSRVAAFRGLFSQVGGVPVTFATAAE
                     FTVIPEIVADPEVAIDSSRVLHGSQEYEFRRPLREGERLAIKSRIESIRSLGASSFLV
                     LATELVEPGGDIVCTARSTLIERAGD"
BASE COUNT         1332 a         2761 c         2776 g         1331 t
ORIGIN      
        1 cctgatctcg atggccatga acggctgggg tgcggccgag ccggcctggt ggctgaacct
       61 gcaggctcat ccggaggcgg tcgtcgagct ggccggtggg atccggcgag aagtgctagg
      121 cagagccgcg gtgggcgtgg aacgtgagca gctgtggcag cgctggcgcg agatcgacga
      181 gaagctcgac ggcttcgctg cgcgacgcgg gcacgaaacg gcggtcgtca tcctcgagcc
      241 gcgccagatg gctggatgac ctgtgccggc acttggtggc ccgctcgggc gggatgggca
      301 tccctccaca cttgtgtccc atccctggaa gcggcgctcg cgcacgatcg cccggctctc
      361 cgatgagggt gggcagatct gatgagcgat gaacggacgc gcgtcctccg ccagctcgcc
      421 gagggtggtt gcgtcgcacc cgaggccgaa gccgacagcc tgctcatcgc ggcgcgagag
      481 ggcgtcggtc cgatcgagga gctggttcgc cggcggcttc ggggtgagcc actcgcgtgg
      541 atcaccggct cggtggcgtt ctgcggtcat caggtccgga tcgaccccgg cgtgttcgtg
      601 ccgcgtcccc acacggaggc gctggctcgg cgggcgatcg agctcctgcc ggacgacggc
      661 atcgccgtcg acctctgcac cgggagcggg gcggtggcag tggcgctcgc cgccgcccgt
      721 ccacgcgcca ccgttgtggg gacggacgtc gacccccagg cgatcgcgtg cgcgggccgg
      781 aacggcgtgg cggtatccga gggcgacctc gacgtgccct tgcctttcac gctccgcgga
      841 cgagtcgacc tgatgaccgc cgtcgtgccc tacgtgccca ccgaggagct ccacctcctg
      901 ccgagagacg tgctcacgca cgagccacgc cgcgccctcg acgggggatc gggcggaaca
      961 ttcgttctcg tgcgtgccgc aggggcggcg gacaggtggc tccgtccggg cgggaccgtg
     1021 ctcctggagc tcggcggcga ccaagctgca gatatgaccg cgatcctcac cgatctcggc
     1081 ttcacggacg tccgtgtgca cacggacgcg gacggccagg atcgcgcgat cgagggccga
     1141 ctggggccgc taccgccggg cgcggtcggg cggggttgag tcgccgacgt tcgactcgag
     1201 ccagtcgacg agcggcttcg accgctcgag gaacgtcacg atccggtcct tcgctctctt
     1261 cgttccgagc caggcccccg ctggccattc ctgccacgcg atcaatccct tgaaccgaag
     1321 gagctcgatc cgtgggtggt ccttgggata gcctttcggc gccgtcttca acacatcgtg
     1381 cccgtggacg tccaggccct gtgttcgcgc atctgacacg atccgtgtca gatcctcacc
     1441 ggacgggtct cgcgcgacgc tctcgcgata gcgctcgagc tggtcggggg ccatctccca
     1501 cctcccgctg ccgacggcga gaccgcgggc gtcgagctgg acgtacccgt cgccgagcgt
     1561 ggccccgatc gcggtcttgt acggcgactt gtcgcggctg aagcggatgt cgcgatacgg
     1621 gcggaagatc cggccctcac cgaactcggg ctccagctcg gcgagcagct cctccatcgg
     1681 tgcgcgcacg atcgtgtcgt acacgtcctt gtggcgctgc cagtacgcct tcgagttgtc
     1741 ggcctccagg ccttcgaaga actcgagcgc ctccaccttc catccgtgga acgccatcgg
     1801 atcccttctc ctcgaccccg atacccggat caggtgcagg ctaccgtccc tccgtcctgg
     1861 aaggccggct cgccgggaac ggacacccat ccgtagtcgt acgagtcctc gtgcagcgtc
     1921 atctgcagca cgccgaaggc cttgttctgc gtgacctcga ggttctcggg catcggaggg
     1981 cgaccgaagg ggtagagctg ccgcccgccc gttcccaccg tgaactggat agtgccgccg
     2041 gcgacggcgt tgccgtgggc gtcctgcgga gcccaccgct ggtagagatg gttgtgtccg
     2101 acgagcacga catcgacgcc ctcgtcggcg aacacctccc agagtgggat gagcggcctg
     2161 gtctccgagc cgccgaaatt gatggtggcg tcgtcctcga tccacttctg gaatggccgc
     2221 cagtcgtagc gagggtgatg catgaacgcg agcgtgcagg tcgcatccgc gttcgcctcg
     2281 aggtccgccc ggagccagtc gccttggggc gtacccgggc cgcagccggg gtcgtcgccg
     2341 cagatgtcgg agttgagcga cacgatgtgc cagtcaccga ggtcgaagga gtagttcccg
     2401 agcggcgggt tggctaccgc gccgaagtag gcgaagtagc cggcggcgtc ggtcgtcccg
     2461 tattcgtggt tccccggggc cggcatggtg atctcccgaa ggtcgccgaa ttgcacgtcc
     2521 cagacgcgca ggaactccga gagcttcccg ttgttgtact gaagatcgcc cagcgcgagg
     2581 aaccggtccg ggtcgatctc gcggatcagg tccgcgacgt cacccgatcg gcaggcaccc
     2641 atgccctgcc cctgcgtcag cgactggcag gcgatgtcgc caacggcgac gatcacggga
     2701 tcgcgatctg tcgcgccggc gccgggtgcg aggagaccga tcgccgtggc cagcgcgatc
     2761 atcgcagcgg agagacggag cgagcgggac gagcgacgca tcggaactcc ttccgggcgg
     2821 tggcgcgtat tcaagcatgc cgagcgcggg gattggaacg cctccgggcg tgcatccgac
     2881 ctcggcgtca gaacgagccg tagaggtcgc tcgccgggtc gaagaagccg ccggccgggc
     2941 gggcgtgcgc gtgccagtgc tccgggatcc ggcgtcgctc ggggtcgatc cagtagccgt
     3001 ccgacccata ccgatcggtg gcgatccgct cgagccgggc caacagctcc gtctcgagct
     3061 cggactcggg cagcccgtgc gagcgccaca cgatcatcgg ggttcggcac acgaggcact
     3121 ccgcgaccca gcactcgtcg tcctcgtgga gccacgcggt gacctgttcc gcggcgcaga
     3181 gctggcagtc atccatcgag ggccctccgg agctccgccc ggtcgccggc accgacgccg
     3241 tgctcgtcga accagcctag gttcgcttcg atcgcgagga cgaacggctc gtcgatcccg
     3301 tacgaggggc aggcgtccgt ctcacacggc tgcatgtcca ggacgtcgat cacgtacccg
     3361 gactcatcga cgaaggcgac ggagagcggc acgagcgtat ccttcatcca gaacgtgctg
     3421 tcgacgggcc cgggccacac gaacgccatc ccctgatcgg cgggaagctc ctcgatgccc
     3481 atcaatcctt tccggcgttc ctgcggatcg tccgcgaggt ccaccgtcag gatcgcgtcc
     3541 gttcctccga acgtcatcgt cgaccccgga atggtctccg gggcctcggg tgtgtcgtca
     3601 gtgcacgccc ccaggagcgt cagcgtgacg atggccgcga tgatctgccg caccacctca
     3661 tcgtgccacg gtgcgcggcc gacccattgt tatcatcgag cgctgtcgcc ggagtagctc
     3721 agtcggcaga gcgcctccat ggtaaggagg aggtcagggg ttcgattccc ctctccggct
     3781 ccagaagaag ccaggttcct cggatgccga ggtaggccgt ccatcgttca tcgtccatcc
     3841 ccgctgcgca gtctcaaggt ggtcgcactc cgccgagact ctcggcagga agaaggggat
     3901 gggggtggac ggtcctagcc gatagggttt ccttcgagtg ctccagcgga ctgtggacag
     3961 acggatggct atacggacag gacggcgccg tggtcggatg cccgtcccga tcgccctgct
     4021 tgctgcgctg ctgatcacgc agatcccgtg ggatgcaccg catgcgcttg cctccacggc
     4081 agacgaggca ccgtccccct cgccggatcc gagcccgtca tccgatccga gcccctcgcc
     4141 ggatccgagt ccctcaccgg atccgagccc atcaccggat ccgagcccat caccggatcc
     4201 gagcccttca ccggatccgt caccctcgcc atccgagacc atcccggtgc ggggtgttcg
     4261 gatcgtgccg cccttcggcc aggagtgggg atcgggagaa cacgctccct cggtgagtgg
     4321 atcctcggga tcgcatttcg accaaccgtt ggagggtggc tcgccgaacg acaccccaac
     4381 gccagacctt tcgacggagc agctgctcgg cggcacatac gcgacccacg acctcgtggt
     4441 ggtggcgggg ctgctcagag cggccggctg gtcggaggaa cgcatccggc gggaggtgta
     4501 cgcgccattc atcgtggtcg ggccggcgag ctgggccgat tcttggcacg ccgttcggta
     4561 cggaccggga ccggccgtgc ggcagcacga agggcaagat gtgctctgcc gttccggcag
     4621 tccggttctg gctcccgagg atggcacgat cgagttcgac accagcttgc tcggcgggcg
     4681 gtcggcccgt ctcctgcttc cgggcggcgg gcactactac ttcgcccact tcagcgactg
     4741 gaacggtcgc gagttctcga acggagacga cgtccaacgc ggtgacgtga tcgggttctg
     4801 cggcgacacc gggaacgcca ccgtcgcgca tgtccacttc ggctggtacg gctcggacgg
     4861 cgtcgcgatc gatccgatgc ggcgactgat ccgatggctc cgggaatccg aagcagacct
     4921 ccctgcactg ctctccgagg ccgggctcgg cggagcagcc acggtttccg agatgctcgg
     4981 atctgcggaa ccgtcgttcg acgtggtcct tgccctcctg gaagggcggc tcgcggagtc
     5041 gcgtttcatc ggatggagga acgcatcggc gaccggatcg cacggcccgc tcgacctgac
     5101 accgctgctg ttcctcgcgc tcgctgccct gacccgtcgg ggaacgacgc ttggatctcg
     5161 gcggcgccgc cgcccgatcg gagctccgat ctggacggtc ccgcccgaat gggaggaccg
     5221 tcgaggaccg ctcctttcct aaggcggtgc aggggtagcg gccgtggcac cgtatccgct
     5281 gctcacatcc ggggctaggt tcgtgccgcg gtcgcaccgt cgcaggtaga ctgagcgacc
     5341 gccgggcggg gtagctcagt ggtagagcaa gcggctcata atcgctgtgt cggcggttcg
     5401 atcccgcccc ccgctacgaa gagagccgag tagacctccg aggagatcac gagacatggg
     5461 caagaagaag ttcgagcgga cgaagccgca tgtgaacatc ggcacgatcg ggcacatcga
     5521 ccacggcaag acgacgctga ccgctgcgat cacgaggcgc caggccgaga aaggcatggc
     5581 ggacttcacg ccgttcgatc agatcgacaa ggctcccgag gagcgagagc gcggcatcac
     5641 gatcgcgatc gcacacgtcg agtacgagac cgacaatcgc cactacgccc acgtcgactg
     5701 tcccggccac gccgactacg tgaagaacat gatcaccggc gccgcccaga tggacggcgc
     5761 gatcctcgtg gtctccgctg ccgatggccc catgccgcag acccgcgagc acgtgctcct
     5821 cgcccgccag gtcggggttc cctcgatcat cgtggcgctg aacaagtgcg acatggtcga
     5881 cgacccggag ctcctcgacc tcgtcgagct cgaggttcgc gagttgctct cgtcctacga
     5941 gttccccggc gacgacgttc ccgtggtgca gatctccgcc ctgaaggcgc tcgagggcga
     6001 cgagacgtgg aacggcaaga tcgacgagct gctcgccgcg tgtgactcgt acatccccga
     6061 accgacccgc gacacggaga agccgttcct gatgccgatc gaggacgtgt tctcgatcac
     6121 gggccgtggg acggtcgtga ccggccgcgt cgagcgcggg acgctcaaca agatgagcga
     6181 aatcgagatc gtcgggatca aggacacgcg caagacgacg gcgaccgacc tcgagatgtt
     6241 ccgcaagctc ctcgacgagg tccaggccgg cgacaatgtg gggatcctgc ttcgcggcat
     6301 cgacaaggac gacgtcgagc gcggacaggt cctggcgaag ccgggctcga tcacccccca
     6361 tacggagttc gaggcgcagg tctacatcct gtccaaggaa gagggcggcc ggcacacgcc
     6421 gttcttcgac ggctaccgtc cccagttcta cttccggacc accgacgtga ccggccaggc
     6481 acacttgccc gggggcgtgg agatggtcat gcccggggac aacgtggaga tgaaggtcga
     6541 catgatcgcc ccgatcgcga tggaagaggg tctgcgattc gcgatccggg agggcggccg
     6601 caccgtcggc gcgggcaggg tcaccaaggt cgtgaagtag cgatggcccg gaaggaaggc
     6661 aatcgcccga agatcacgct ggcctgctcg gattgcaaga ggcgcaacta caacacggtg
     6721 aagaaccgtg tgaacgaccg ggaccggatc gagctcaaga agtactgccg ttggtgccgc
     6781 tcccatacgc tgcaccgcga gacgcgctag cgctggtcca agcgctcaag tagcgaagcg
     6841 agagcgcaga cccaagcgct caagtagcga agcgatagcg cagacccaag cgctcaagta
     6901 gcaagcgata gcgcagcttc aacgaacgga tcagcccgcg tcgacgtccg tctccgccag
     6961 cttctcgttc gtcttgacct tgccgaagcg gatcgccgcg acgacggcgt cggtcttgga
     7021 gtgcacaccg agcttggtca agacgttctg gatgtgcgtc cgcagcgtgt gacgactcat
     7081 cccgagctcg ttcgcgatgt cgaccgatgc ctcgcccgca gcgacgcgct ggaggatctc
     7141 gagctctcgc ggtgtcaggc gctccacccg ctgcgcgagc tcgccatcgg ttcgccgctg
     7201 catccggaac cgcgccaagg agagctcgac ctcactcacc tcgtggagcg gatcgccacg
     7261 gttcgcgcgg cggatcgcct cggcgagctc ggagacggcc tgggtcttcc gcaggaaccc
     7321 ccgggctccc gcttcgacgg cacgcgcgag cacgacatcg tcgtcgtgac cggacagcag
     7381 gatgaccttc gtgttcgacg cctcccggtg gatgcgccgg gtggcttcca ggccgtcgac
     7441 gccgggcatc cccacgtcca tcaggagcac atccgggtgc tgcacctggg cggattcgat
     7501 cgcggactca ccgtcggtga ccacctcgat caccttgaga tcgtcctcgt tgtccagcgc
     7561 gatctgcagg gcctccccga aggagcggtg gtcgtcggcg atgacgacgc ggatgtcccc
     7621 ctcggctgtc ccagatgcct tgcggtcctt cggcctggct cgggcagcca taccgcggca
     7681 tcgtaggcat gttggtttac ggaatcacgt acccccagcg ccggattttc tcgagtcccc
     7741 cgtccgaggg acgccggacg ccgtccgacc gacgttggat tagcatcccc cgatgaacgc
     7801 ggcagccgcg gggaaggtct atccggaggc acccttcaca gtggatccca gccgtgtggc
     7861 cgcgttccgg ggactgttct cccaggtggg aggggtcccg gtcacgttcg cgaccgcggc
     7921 ggagttcacc gtgatccccg agatcgtggc cgatccggag gtcgcgatcg actcctcccg
     7981 ggtcctccac gggagtcagg agtacgagtt ccggcgcccg ctgcgggagg gagagaggct
     8041 ggcgatcaag agcaggatcg agtcgatccg ctcgctcgga gcaagttcct tcctggtcct
     8101 cgccaccgag ctcgtggaac ccggcggcga catcgtctgt acggctcggt cgacgctgat
     8161 cgaacgggcc ggggactgat gccgccgccg acccgggtcg
//
LOCUS       DASUVE010000076         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_412954, whole genome shotgun sequence.
ACCESSION   DASUVE010000076 DASUVE010000000
VERSION     DASUVE010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412954"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="VF036_01565"
     CDS             complement(<1..1568)
                     /locus_tag="VF036_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX6263948.1"
                     /translation="MNVAPGVQNVSISTVCLSGTLEDKLRAAAEAGFAGVELLEYDLV
                     MSPWSPGRLAEEAAGLGLSVDVYQPFHVETVPSELFGASLRHAERKFDLLSELGARVL
                     VCCSSRSPMGLDDDDLTSEQLRALADRAAQRGLRLAYEAVPWGRVRTHEDAWRIVQQV
                     DHPALGLCLDSFHVLSLDNDPAAIARVAGDKVFHVQLADAPRLNMDVREWSLHYRMFP
                     GQGSLDVAGFLSRILAMGYSGPIALEVFNDVYQQEDPRHAATDAMRSMLALAEAVATR
                     GSGAVPQFAHVDVPPAPRLSGFAFAELAVDEVSAPLVSRALATLGFAHIGQHRSKPVQ
                     LWEQGEARLLLNLAPERSIDPATASISALAVDSADPTASMERAQRLLAPKLARSRGPD
                     ETDLRSVATPDGTALLFCDSTRRPTWLDEFTSTGRPAGPSALLRDIDHVSITESIDDF
                     DQSALFFRAVLGLVPRESTEITAPFGLIRSWSASDPSGRVRVMLSTTPLRRGDWAPGV
                     SSPQLVAFASDDAIA"
     gene            complement(1618..>2621)
                     /locus_tag="VF036_01570"
     CDS             complement(1618..>2621)
                     /locus_tag="VF036_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007961533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6263949.1"
                     /translation="SALVLGMTGFWGTAFTNETTRTADWILALGTRFAEADSSSWYAD
                     YTFDIPATKLMQIDIDPAELGRNYPLEIGALADLKSALTVLVRAARRLAPAGVRRDEL
                     LAQIAVNRATVKLQNADAQISDAWPMRPERILSETREVLPADAIITTDVGWNKNGVGQ
                     QFDILTPGTFLTPGGFATMGFGAPAAVGAKIARPDRLVVSLVGDGGFGQNPAVLATAR
                     EQNVPVVWVVMNNNAFGTIAGLEKAAFDTTYGTVFGNADDPMYTDFAAIAEAYGVTGI
                     RVDSAAEFKPALQRAVALGGPVVIDVSMVNVPTPTTGHWNILDIYSPGETVEHVATH"
BASE COUNT          434 a          845 c          940 g          402 t
ORIGIN      
        1 gcgatcgcat catcggatgc gaaggcgacg agttgagggc tcgacacccc gggcgcccaa
       61 tcgccacgcc tcaggggggt ggtgctcaac atgacgcgca cgcgaccgct gggatcgctc
      121 gccgaccagc tgcggatgag gccgaagggc gcggtgatct ccgtggactc cctcggcacg
      181 aggccgagga cggcccggaa gaacaacgcg gactgatcga agtcgtcgat cgactcggtg
      241 atcgacacat gatcgatgtc ccggagcagt gcggacgggc cggccggtct cccggtcgaa
      301 gtgaattcgt cgagccaggt cggccggcgg gtggagtcgc agaagagcag agcagtgcca
      361 tccggcgtgg ccacagatct caggtcggtc tcatcaggtc cgcgagatcg ggccagcttc
      421 ggcgcaagca gcctctgggc ccgttccatc gaggcagtcg gatccgcgct gtcgaccgcg
      481 agcgcactga tcgatgcagt ggcagggtcg atcgatcgtt ccggggcgag attgagcagg
      541 agccgagctt ccccctgctc ccacaattgc accggcttgg agcgatgctg cccgatgtgg
      601 gcgaacccga gggtggcaag ggcacgcgaa acgaggggag ccgacacctc gtccaccgcg
      661 agctcggcga acgcgaaccc gctgagccgc ggcgccggcg gtacatcgac gtgggcgaac
      721 tggggcactg ctcccgagcc gcgcgtggcc accgcctcgg cgagcgcgag catggagcgc
      781 atcgcgtcgg tggcggcgtg cctggggtct tcttgctggt agacgtcatt gaacacctcg
      841 agggcgatcg ggcccgaata gcccatggcg aggatccgac tgaggaagcc cgcgacgtcg
      901 agcgagccct gcccggggaa catccggtag tggaggctcc actcgcggac atccatgttc
      961 aatcgaggtg catcggcgag ctggacgtgg aacaccttgt cgccggccac gcgcgcgatg
     1021 gcggcaggat cgttgtccag tgacaggaca tggaagctgt cgaggcacag ccccagcgca
     1081 gggtggtcga cctgctggac gatgcgccag gcgtcctcat gggttcggac tcgtccccac
     1141 ggcacggctt cgtacgcgag ccgcaatccg cgttgcgcgg cgcggtccgc gagggcacgc
     1201 agctgctccg aggtgagatc gtcgtcgtcg agtcccatcg gagacctcga cgagcagcac
     1261 accaggacgc gcgcgccgag ctcggacagg aggtcgaact tccgctcggc gtgacgcagg
     1321 ctggcgccga acagttcgga ggggaccgtc tcgacatgga acggctggta cacgtcgacc
     1381 gacagaccga ggcccgcggc ctcctcggcc agtcggccgg gagaccacgg cgacatgacg
     1441 aggtcgtact cgagcagctc cacgccggcg aagccggcct cggcggcggc gcgcaatttg
     1501 tcctcgaggg tgccggacag gcacaccgtc gagatggata cgttctgcac gccgggtgcg
     1561 acgttcatga gcgggccgcc tgacggatcg gtgggtccag ggtggtgaat gcggagatca
     1621 gtgtgtcgcg acgtgctcga ccgtctcgcc gggcgagtag atgtcgagga tgttccagtg
     1681 ccccgtcgtc ggcgtcggca cattcaccat cgacacgtcg atcacgaccg gcccgccgag
     1741 cgcgaccgcc cgctggaggg cgggcttgaa ctccgctgcg gagtcgaccc tgataccggt
     1801 gaccccatac gcttcggcga tcgccgcgaa gtcggtgtac atgggatcgt ccgcgttgcc
     1861 gaagaccgtc ccgtaggtgg tgtcgaaggc cgccttctcg agccccgcga tggtgccgaa
     1921 ggcgttgttg ttcatcacga cccagacgac ggggacgttc tgctcgcgcg ccgtggcgag
     1981 caccgccggg ttctgtccga agcccccgtc gcccacgagc gagacgacga gacggtccgg
     2041 ccgggcgatc ttggcgccga cggcggccgg cgcaccgaag cccatggtcg cgaagccacc
     2101 cggtgtgagg aacgtgccgg gggtgaggat gtcgaactgc tgaccgaccc cgttcttgtt
     2161 ccagcccaca tccgtggtga tgatcgcgtc cgcgggcagg acctcccggg tctcggagag
     2221 gatccgctcg ggccgcatcg gccaggcgtc ggagatctgc gcgtccgcgt tctggagctt
     2281 caccgtcgcg cggttcacgg cgatctgcgc gagcagttcg tcgcgccgca cgccggcagg
     2341 ggcgagtcgg cgcgcggcgc ggaccagaac ggtcagcgcc gacttgagat ctgccagcgc
     2401 gccgatctcg agcgggtagt tgcgccctag ttcggcagga tcgatgtcga tctgcatgag
     2461 cttcgtggcc gggatgtcga aggtgtagtc cgcgtaccac gaactcgagt cggcctcggc
     2521 gaagcgggtt cccaatgcga ggatccagtc ggccgttcgc gtcgtctcgt tggtgaacgc
     2581 cgtcccccag aatcccgtca tgccgagcac cagggcgctg t
//
LOCUS       DASUVE010000077         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_977795, whole genome shotgun sequence.
ACCESSION   DASUVE010000077 DASUVE010000000
VERSION     DASUVE010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977795"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VF036_01575"
     CDS             complement(<1..389)
                     /locus_tag="VF036_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6263950.1"
                     /translation="MPHEPFLLASIEVVPLCDGWAPLPLDDEAPGHAVDWDEQRSRFP
                     WAFPPDDATSWAWHVHSFLIRHPDGPVLVDTGIGDLGRPPYDVAGRLEDELASIAVSP
                     DSVRHVILTHLHGDHSGGACATDGSPT"
     gene            complement(399..980)
                     /locus_tag="VF036_01580"
     CDS             complement(399..980)
                     /locus_tag="VF036_01580"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX6263951.1"
                     /translation="MLAGGRSRRFGRDKLAEEIDGRPLLHHAILRLLDVCDRVVVVIG
                     VDSEEPPVPLGATVTFARDSTADEGPLRGLAAGLEVAGTTWAVVAGGDMPDLQPPVLG
                     EMLRAARETAAVAVVLSDGGDARPLPCVVRTEPAADAAAVLLGSGRRRLRGLLSTVRT
                     VVVDEPSWTALDPGRRTLVDIDEPGDVRRGPPR"
     gene            complement(994..1956)
                     /locus_tag="VF036_01585"
     CDS             complement(994..1956)
                     /locus_tag="VF036_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019609882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX6263952.1"
                     /translation="MSQAVPLDPPRRLLDLEIDGRTTRVPEGATILDACRAEGVDTPT
                     MCYAGNLTPVNACRVCMVEVEGSRVLVPACSRPVEDGMKVQTDTERVRHSRKLVMEFL
                     ASSVDTSLASSDWLRWMGEYGADPSRYGAPMEPMESGERDERWAGHHHDPDPAIAETV
                     AQPVKVDNELYVRDYERCVLCYKCVEACGVDAQNTFAIAVAGRGFDARISTEFAVPLD
                     ESACVYCGNCIGVCPTGALMFTSEHDMRAADTWDEDAQTVTNTICPYCGVGCELEVHV
                     QDNEIVKVTSPEDHSVTSGHLCIKGRFGFAYVQSRRGRDDRRPR"
     gene            complement(1953..>2338)
                     /locus_tag="VF036_01590"
     CDS             complement(1953..>2338)
                     /locus_tag="VF036_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX6263953.1"
                     /translation="FVGPDMLDARLTFEDARQGGYTLGSGVIMVFDEHADLVDLCLRI
                     ARFFRDESCGQCVPCRVGTVRQEEALHRLVARTPIGSSRDELALIDEVARVMRDASIC
                     GLGQTAANAVQSAISKLGVFDGGAA"
BASE COUNT          360 a          842 c          795 g          341 t
ORIGIN      
        1 aacgtcgggc tgccgtcggt cgcgcacgca ccgcccgagt ggtcgccgtg cagatgcgtg
       61 aggatcacgt gccggacgct gtccggagat accgcgatcg aggcgagctc gtcctcgagc
      121 cgccccgcga cgtcgtacgg cggtcggccg aggtcgccga tccccgtgtc cacgagcacc
      181 ggtccgtccg gatggcggat caggaaggag tgcacgtgcc acgcccagct cgtcgcgtcg
      241 tcgggaggga aggcccacgg gaaccgggac cgttgttcgt cccagtcgac ggcgtggccg
      301 ggcgcttcgt cgtcgagcgg cagcggtgcc catccgtcgc agagaggcac gacctcgatc
      361 gatgcgagca ggaacggctc gtgcggcacc ccatgagcct agcggggagg cccgcgccgg
      421 acgtcgcccg gctcatcgat gtccacgagg gtccgacgcc ccgggtcgag ggcggtccag
      481 gaaggctcgt ccaccacgac ggtcctgacg gtcgagagga gaccacggag cctgcgtcgt
      541 ccgctcccga ggaggaccgc ggcggcatcc gcggccggct cggtccgcac gacgcaggga
      601 agcggtcgtg cgtctccacc gtctgagagg acgaccgcga cggcggcggt ctcccgggcg
      661 gctcgcagca tctccccgag cacgggcggt tggagatccg gcatgtcgcc gcccgccacg
      721 accgcccacg tcgtcccggc gacctcgagt cccgcagcca gcccgcggag cgggccttcg
      781 tcggcggtcg agtcacgcgc gaaggtgacg gtggcaccaa ggggcacggg cggttcctcg
      841 ctatcgacac cgatcaccac gacgacgcga tcgcagacgt cgaggagccg gaggatcgca
      901 tggtggagca acgggcgacc gtcgatctcc tcggcgagct tgtcgcggcc gaatcgtcgg
      961 gagcgccccc ccgccagcac gatcccggcg acgctatctc ggtcgtcggt cgtcgcgacc
     1021 ccttcgactc tgcacgtagg cgaacccgaa ccgtcccttg atgcagaggt ggccgctcgt
     1081 cacggagtgg tcctccggcg acgtcacctt gacgatctcg ttgtcctgga cgtgcacttc
     1141 cagctcgcat ccgacgccgc agtacgggca gatcgtgttc gtgacggtct gcgcgtcctc
     1201 gtcccacgtg tccgcggccc gcatgtcgtg ctcggacgtg aacatcaggg caccggtcgg
     1261 acagacgccg atgcagttcc cgcagtacac gcaggcggac tcgtccagcg gcaccgcgaa
     1321 ctccgtggag atcctggcgt cgaagcctcg accggccacc gcgatcgcga acgtgttctg
     1381 cgcgtcgacc ccgcacgctt cgacgcactt gtagcagagg acgcatcgct cgtagtcgcg
     1441 gacgtacagc tcgttgtcca ccttcacggg ctgggcgacg gtctccgcga tcgccgggtc
     1501 ggggtcgtga tggtgaccgg cccagcgctc gtcgcgctca ccgctctcca tcggctccat
     1561 cggggccccg taacgcgacg gatccgctcc gtactccccc atccatcgga gccagtcgga
     1621 ggaggcgagg ctcgtgtcca cgctcgaggc gaggaactcc atcacgagct tccgcgaatg
     1681 acgcacgcgc tcggtgtcgg tctgcacctt catcccgtcc tcgacgggcc gggaacacgc
     1741 cgggaccagc acgcgggagc cctccacctc gaccatgcag acccggcagg cgttgaccgg
     1801 cgtgaggttg cccgcgtagc acatcgtggg cgtgtccaca ccctccgccc ggcaggcgtc
     1861 caggatcgtc gccccttcgg gaacccgcgt cgttcgaccg tcgatctcca ggtcgaggag
     1921 tcgccggggt gggtcgagcg gcacggcctg gctcacgcgg cacccccgtc gaagacaccc
     1981 agcttggaga tcgcggactg gacggcgttg gccgccgtct gtccgaggcc gcagatcgac
     2041 gcgtcgcgca tcacgcgggc cacctcatcg atcaaggcga gctcgtcccg ggaggagccg
     2101 atcggggtcc tcgccacgag tcgatggagc gcttcctcct gccgcacggt ccccacgcgg
     2161 cacggcacgc attgcccaca gctctcgtcg cggaagaacc gtgcgatccg caggcagaga
     2221 tcgacgagat ccgcatgctc gtcgaagacc atgatcacgc cggagccgag cgtgtagccg
     2281 ccctgccgcg cgtcctcgaa cgtgagcctc gcatcgagca tgtcgggacc gacgaacc
//
LOCUS       DASUVE010000078         4774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_36325, whole genome shotgun sequence.
ACCESSION   DASUVE010000078 DASUVE010000000
VERSION     DASUVE010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36325"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VF036_01595"
     CDS             complement(<1..873)
                     /locus_tag="VF036_01595"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX6263954.1"
                     /translation="MRRIVFGLSLILIFTIPWEGAFHLPGLGTTATVMGLIVGACWLF
                     MIVVASQMRRPVLFLFAVAGFVAWVALTTFWSPDPLESLGAVYLWLQTLLFVYILWDL
                     YRTKAALLAGLQAYVLGAYTAVGGAVLNYVQSNAFYTNEERYSLGNTNPDGYGFILAL
                     GIPVACYLAASPETPKVFRFINIGYLPVAFIGVALSGTRTASVGAAIGLLYGLATLTK
                     LKIHTRVAVVVVLAASLFVLLPIVQPLSSFQRLGTTGTEVAEGDLNGRTGQWEQGLRA
                     FMEHPIQGVGTDMYR"
     gene            complement(876..2027)
                     /locus_tag="VF036_01600"
     CDS             complement(876..2027)
                     /locus_tag="VF036_01600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6263955.1"
                     /translation="MNVSADATAGGSTSSTTDARVRVAIFTANMDGGGAERAMAKLAA
                     GIADRGFDVDLVLSDASGHYLAEVPAEVRIVDLHARRVLASLPGLVRYLRRERPAAML
                     TSMNYVNVVGIWARALARVDTRLVVNEQNALSLEAAHSPRRRHRMLPRLIGRFYPWAD
                     RIVSVARGTADDLSRTAGLPRERIDVVHNPIVTPELKQLADAPLDHPWFRPGEPPVVV
                     SVGRLAEQKDFGTLLRAFARVREDRDARLVILGEGPERGSLEALVAELGLDAAAALPG
                     WVQNPYPWMKGAGTYVLSSRWEGLPSVLIEALYCGVPIVATDCLSGPQEILDGGRHGL
                     LVEVGDVDGLARGIGSALAGEVPAPAPESWRPYEQEFVVSRYLEVLVGE"
     gene            complement(2024..3559)
                     /locus_tag="VF036_01605"
     CDS             complement(2024..3559)
                     /locus_tag="VF036_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX6263956.1"
                     /translation="MVSRVVSDGSLTKKASLNAAASTAEQGARIVAGLIISPILLTHL
                     GDTVFGIWQVLQRLIGHASPASGRPGEALKWTIAHEQRSTDYEFKRRQVGNALAVWVL
                     FLPLLLAIGGVLAWLSPVWLDVPPDSFGTVRLAAGILVLDAVLFSLLYLPQSVLQGEN
                     LGYKRLGLSVLLVFVSKSLLVVAVYFGAGIGGLAVATVVATVLWGLTYLLIVRSRVEW
                     FGIARPSIAEVRHFVGLSWWFLLWNLVMTIMLASDVVVLGVVGSPALVTTYTLAKYLP
                     QAIIVAVATLIVAIMPGLGGMIGAGELARAVRIRRETMSLIWLFTIVAGTTVLLWEAS
                     FLQLWVGEQYYPGSGAVLVIMLMVLQFGLIRTDANIIDLTLNLRTKVLLGAISAVASI
                     ALAWLFIGPMELGITGLVLGFIAGRTIQSIGYPLMIGRLLEIPPERQLRGIVRPAIVT
                     AVLFGGAGALADSLHAGSWVALVLSVGLSVTILAPVVFLAGLQPEVRRLLWLRLRRVA
                     RLT"
     gene            complement(3633..>4774)
                     /locus_tag="VF036_01610"
     CDS             complement(3633..>4774)
                     /locus_tag="VF036_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263957.1"
                     /translation="YVASARPEGMFPIPTTAFELLLFLIRMVVKHSTWDAFVSFQGSL
                     SKSERRELEDLVERTDLHEVWELAERHLPFIPRELLERCLRCVQPGASRWLQIRTASR
                     LQRALAGCSRRPPVLDTGLRMWRRGRTVVRRKVLRRGPVRSQLSSGGALVAIVGGDGA
                     GKTTVVDGLSSWLASEHIDSSTMHMGKPSWSALTAIVKGSLKLAAATKRTSTSSAKAL
                     RSSLSTGEGGLTVRNRARLVWEVLTARDRFRTYRRARRLATNGAIVICDRYPLPEVSL
                     MDGAVTGRLQDPSALGRWAAWLAGLERRYYERIRDPDTLIVLRVDPDTAVERRRGIEP
                     ESSVRPRSEEIWGIDWSGTSAIVIDAASPKDDVLSEVKATIWSRL"
BASE COUNT          891 a         1648 c         1575 g          660 t
ORIGIN      
        1 tcggtacatg tccgtgccga cgccctggat agggtgctcc atgaacgctc gcagcccttg
       61 ctcccattgg ccggtgcggc cgttcaggtc tccctccgcg acctccgttc cggtcgtccc
      121 gaggcgctgg aaggaactca agggctgcac gatcgggagc aggacgaaca gggatgcggc
      181 caggaccacc acgacggcga cccgagtatg gatcttcagc ttcgtcaacg tggcgaggcc
      241 gtacaggaga ccgatggccg ctcccaccga cgcggtcctc gtgcccgaaa gtgccacccc
      301 gatgaacgcc acaggcagat agccgatgtt gatgaaccgg aagaccttcg gggtctccgg
      361 cgacgccgcc aggtaacagg cgacggggat gccgagcgcg aggatgaagc cgtatccgtc
      421 cgggttggtg ttgccgaggc tgtaccgctc ctcattggtg tagaacgcgt tggactgaac
      481 gtagttcagg accgcacctc cgacggcggt gtacgcaccg aggacgtacg cttgcagtcc
      541 cgcgagcaac gccgctttcg ttcgatagag atcccacagg atgtagacga acagcagggt
      601 ctgcagccag aggtacacgg cgccaaggct ctcgagggga tcggggctcc agaacgtcgt
      661 gagcgcgacc cacgcgacga acccagccac ggcgaacagg aacaggacgg gccgtcgcat
      721 ctggctggcg acgacgatca tgaacagcca gcacgcgccg acgatgaggc ccatcaccgt
      781 cgccgtcgtg ccgaggcctg gcagatggaa ggctccctcc caggggatcg tgaagatcag
      841 gatcagagaa agcccgaaga cgatgcggcg catgactact cccccaccag gacctcgaga
      901 taccgactga cgacgaactc ctgctcgtag ggtcgccagc tctcgggcgc cggcgccggc
      961 acctcgcccg ccagtgccga cccgatcccg cgagcgagcc catccacgtc gccgacctcc
     1021 acgagcagcc cgtgtctgcc gccgtccagg atctcctgcg ggccgctgag gcagtcggtg
     1081 gccacgatcg gcacaccgca atacagcgcc tcgatcagca cggacgggag gccctcccac
     1141 ctcgacgaca ggacataggt tccggcaccc ttcatccagg ggtacggatt ctgcacccaa
     1201 ccggggaggg ccgccgcggc gtcgagaccg agctcggcga ccagcgcttc gagcgacccg
     1261 cgttccggcc cctcgcccag gatcaccaag cgcgcgtcac gatcctcgcg gacccgcgcg
     1321 aacgcgcgga ggagcgtccc gaagtccttc tgctccgcga gccgccccac cgagaccacg
     1381 acggggggct caccggggcg gaaccacgga tggtcgagcg gagcgtccgc gagctgcttc
     1441 agctccggcg tcacgatcgg gttgtgcacg acgtcgatcc gctcgcgggg aaggccggcc
     1501 gttcgggaga ggtcatcggc ggtgccgcgg gcgacggaca cgatccgatc ggcccacgga
     1561 tagaaccggc cgatcaaccg cggaagcatc cgatgccgcc gtcgaggtga gtgcgccgcc
     1621 tcgagggaga gtgcgttctg ttcgttcacg accagtcgcg tgtcgacccg ggccagagct
     1681 ctggcccaga tgccgacgac gttcacgtag ttcatcgatg tcaacatcgc cgcgggccgt
     1741 tcccggcgga gataccggac gagacccgga aggctcgcga gcacgcggcg ggcgtgaagg
     1801 tccacgatgc ggacctccgc cgggacctcg gcgagatagt gtcccgaggc atcgctgagc
     1861 acgaggtcga cgtcgaatcc gcggtccgcg ataccggcgg cgagtttggc catcgcgcgc
     1921 tcggcgcccc cgccatccat gttcgccgtg aagatcgcga cgcgcaccct ggcatccgtc
     1981 gtggagctcg tcgagccgcc ggccgtagcg tccgcggaaa cgttcatgtg agtcgagcca
     2041 ccctacggag tcggagccag agcaggcgcc ggacctcggg ctgcaggccg gcgaggaaca
     2101 ccacgggcgc gaggatcgtg accgaaaggc cgacggacag gacgagcgcc acccaagatc
     2161 cggcgtggag cgaatccgcg agcgctccgg ctccgccgaa caggacagcg gtgacgatcg
     2221 cgggacggac gatgcctcgc aactgccgtt cgggagggat ctcgagcaac ctgccgatca
     2281 tcaaggggta gccgatgctc tggatcgtgc gacccgcgat gaacccgagg acgagcccgg
     2341 tgatgcccaa ttccatcggg ccgatgaaga gccatgcgag ggcgatcgac gccacggcgg
     2401 agatcgctcc gaggagcacc ttcgtccgga ggttcagcgt cagatcgatg atgttggcgt
     2461 ccgtgcggat caatccgaac tggagcacca tcagcatgat caccaagacg gcgccggacc
     2521 ccgggtaata ctgctcgccg acccagagct ggaggaagga cgcctcccac aggaggaccg
     2581 tcgtccccgc gacgatcgtg aagagccaga tcaggctcat cgtctccctg cggatacgga
     2641 cggcgcgcgc aagctccccg gcgccgatca tccctccgag cccgggcatg atcgcgacga
     2701 tgagcgtcgc caccgcgacg atgatcgcct gcggaaggta cttcgcgagc gtgtacgtcg
     2761 tgacgagcgc gggcgaaccg accacgccga ggacgaccac gtcgctcgcg agcatgatcg
     2821 tcatcacgag gttccacagg aggaaccacc agctcagacc gacgaaatga cggacctccg
     2881 cgatgctcgg ccgcgcgatg ccgaaccact cgacgcggct ccggacgatg agcagatagg
     2941 tcaggcccca caggacggtc gcgaccacag tcgcgaccgc caatccgccg atccccgctc
     3001 cgaagtagac ggcgaccacg aggagcgatt tgctgacgaa gacgaggagg accgacaacc
     3061 ccaatcgctt gtacccgagg ttctcgccct ggaggacgga ctgcggaaga tagagcaagc
     3121 tgaagaggac tgcgtcgaga accaagatcc ccgccgccag gcggacggtt ccgaaggagt
     3181 ccggcggcac atcgagccac acgggagaca gccaggccaa gacgccgccg atcgcgagga
     3241 gcaggggaag gaagagcacc cacaccgcga gggcgttgcc gacctgccgt ctcttgaatt
     3301 cgtagtcggt cgaacgttgc tcgtgggcga tcgtccactt cagggcctct cctggacgac
     3361 cgctcgccgg tgatgcgtgg ccgatgaggc gctgcaacac ctgccagatg ccgaacacgg
     3421 tatccccgag atgcgtgagg aggatggggc tgatgatcag tccggcgacg atcctcgcgc
     3481 cctgctcggc cgtggacgcg gccgcgttga gggatgcctt cttcgtcagg ctgccgtcgg
     3541 agaccacacg ggagaccatc cggcccagcc gccctctcgg cgcggacctc ggagtcgtgt
     3601 catccgtctt catccgccgt ccctcggcgt ccctagagcc tggaccagat ggtggccttg
     3661 acctcggaaa ggacgtcgtc cttcggcgag gccgcgtcga tcacgatcgc cgacgttccg
     3721 ctccagtcga tcccccagat ctcctcggaa cgcggacgga ccgaggactc cggttcgatc
     3781 ccgcgccgcc gctccacagc cgtgtcgggg tccacacgca gcacgatcaa ggtgtcggga
     3841 tcccggatgc gctcgtagta gcgtcgctcg agtccggcca gccatgcggc ccatcggccc
     3901 aacgccgagg gatcctgcaa ccggccggtc accgctccgt ccatcaggga gacctccggc
     3961 agcggatatc ggtcgcagat cacgatcgcc ccgttcgtgg cgagacgtcg cgccctccga
     4021 tacgtgcgga agcgatcgcg agccgtgagg acctcccaga cgaggcgcgc ccggttccgc
     4081 acggtcaacc cgccctcgcc ggtggacagg gacgaccgca acgccttcgc ggacgacgtc
     4141 gaggtccgtt tcgtcgctgc ggcgagcttc agcgagccct tgacgatggc cgtgagcgcg
     4201 gaccacgacg gcttccccat gtgcatcgtc gaggagtcga tgtgctcgga ggcgagccag
     4261 ctcgagagcc cgtccaccac ggtcgtcttc cccgccccat ctcctcccac gatggcgacc
     4321 aacgcaccac cggaggacag ctggctccgg accggccctc ggcggaggac cttgcgccgc
     4381 accaccgtcc tcccacgccg ccacatgcgc agacccgtat cgaggaccgg cggccgtcgg
     4441 ctgcagcccg cgagcgcgcg ttgcagacgg gacgcagtgc ggatctggag ccagcgcgac
     4501 gcgcccggct gcacgcagcg caggcatcgc tccaggagct cgcgcgggat gaaggggaga
     4561 tgccgttccg cgagctccca tacctcgtgc agatccgtcc tctcgacgag atcctccagc
     4621 tcccggcgct cgctcttcga gagcgacccc tggaacgaga cgaacgcgtc ccaggtcgag
     4681 tgcttcacga ccatccggat caggaacagc agaagctcga aggccgtggt ggggatcggg
     4741 aacatcccct ccggtcgagc cgacgcgacg tacg
//
LOCUS       DASUVE010000079         9881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1355258, whole genome shotgun sequence.
ACCESSION   DASUVE010000079 DASUVE010000000
VERSION     DASUVE010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9881
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355258"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VF036_01615"
     CDS             <1..129
                     /locus_tag="VF036_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6263958.1"
                     /translation="ASRLGISPRTVETHVATLYRRLGVNTRVQAVSQAARLGLVDL"
     gene            169..879
                     /locus_tag="VF036_01620"
     CDS             169..879
                     /locus_tag="VF036_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6263959.1"
                     /translation="MMVSESTIGPSGGKTPTHHDVVIVEPFPVVRAGLGLIVDASPGL
                     TVAATSASAEEAIPLIDRLDRTQLVVLVSMGLAGESDAFWLVRALRERFPGLVILGLG
                     ANADPNTVSRALFTGADGFIDKNVEVEEFLEAIRDASDAEVVIAGPAADSVGTIAEGI
                     ERRRSQTFRLTRREREVLAVAAEGLTAREIADRLGVRERTVTTHLARIYGKLGVGNRL
                     AALRLAARSGLLTLPPAE"
     gene            complement(882..2333)
                     /locus_tag="VF036_01625"
     CDS             complement(882..2333)
                     /locus_tag="VF036_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263960.1"
                     /translation="METPKGADFRAHFRQASVGRRADPPWATLSWALPALAALLAKMR
                     AIDLAYHVRAGELSLRTGDLLLVDPFTFTSGGRPWLNQQWAAQLIFAGAHRLLGWAGV
                     AITYAGAMASGFGLLYRSCRRAKATPRTAAILSLLGFMVASGSLAARPQALAVPLFTG
                     TWLLLERRDRWMWGVPVLAVIWANVHGSFVLAPGLVAFALCEDLLERKDPRPTLLLLL
                     ATAGATFVTPFGGSVWTYALDLVGNETIRSSVAEWRPPSPFSLSGGPFWASVVAVAIV
                     GVSKREEIRLIDVARLLVFFALGVPALRGTLWWALAAPPIVAGWFATREPVDPGQGGE
                     SRRVPTLIATSMVIALGLTLSLRAGTDPATGGPARLAADAPEHLVIATRKALPSGSRL
                     LVFQPFASWFEYSLPEDPVMVDSRIELFPSTVWRDYSRATRAEGGWERTLDRYEIQGV
                     VLPPGEPLGEALAQTPGWTLGAQIPSGSIFVRS"
     gene            2532..2738
                     /locus_tag="VF036_01630"
     CDS             2532..2738
                     /locus_tag="VF036_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009803021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX6263961.1"
                     /translation="MLKLYTGLQTWWADLKGRAQEEHGATAVEYALMVALIAVVIILA
                     VTFIGTSAESKFNQVGSAVNNAGS"
     gene            2822..3250
                     /locus_tag="VF036_01635"
     CDS             2822..3250
                     /locus_tag="VF036_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEX6263962.1"
                     /translation="MDVSIRSKLHREEGAAAVEFALIVGLLAVLIFGLLEYGLAFWQV
                     QNLRAAAREGARVAAVRGDATEVRSHMVASSAGSLSGGWTYTQNRTCDGSDANKGQEV
                     TIQITNSSLSGPVQDAFQVSIPFLPPLQLNPVLRGSFRCE"
     gene            3276..4613
                     /locus_tag="VF036_01640"
     CDS             3276..4613
                     /locus_tag="VF036_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX6263963.1"
                     /translation="MADPHRLHDDERGATAVIVVLSLLALFGLVVLTVDVGQLLFQRR
                     GMVNASDAAALAAAQTCAGLDDSDVPEAVADAFAVNNVGSAITAVPNITEMVGCDGPA
                     YGHVSVEYAMNQNLFFAGVLGISGPASVRTAATAGWGPSGGANPLPIVVYVGNDQGNC
                     DIEEGTPAGVDCYLWYDNDLFENSAFGFLNLCTATDPCSHGWDVAANANCPNVGSSLR
                     DDWIAGNWTGGPNELNYPDPTYVCVVTGLSSSNWVELDRRVGDDLIFPVDDCPTNVDN
                     NGNIVGCDAFPNKYNIVGFITLHLDAVLDSAAEWGGVAQTHCSPNNVNVTPGLVVPLS
                     SFMGGSCPNGSTPSGVLDFRIDDQTSSPNWMYDDLNKQFTWTGPADRVNVDFDWWLDG
                     ECGQPPPNSSAVCIKVHTVEVRFGGTSPGGGADFGLRSIRLCDMQFGSCPEQD"
     gene            4714..5490
                     /gene="cpaB"
                     /locus_tag="VF036_01645"
     CDS             4714..5490
                     /gene="cpaB"
                     /locus_tag="VF036_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX6263964.1"
                     /translation="MVAIAIVLAVGAAAAVVLYTQGVKEEAEQGGQLTTVIVATQEIP
                     ANQRLDPLIEQGLFAEIDVPADALVGGAVTTLTELEGATTTTPILANEQIPTARLSTS
                     EEQLNIVGVSEGHVGLSVELDAPQGGVGNINPGDNVQVFATYQGVSLIPGNLRQYLTQ
                     PADAQAGAKKDVPDFTVTLVPTVRVLRIQNPEVDTETGRSDSSRIQITLDLLPQDAQT
                     LVFAQENARIWLGLLPPNEDGTQPPASTVPIELLLGAKLG"
     gene            5487..6707
                     /locus_tag="VF036_01650"
     CDS             5487..6707
                     /locus_tag="VF036_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263965.1"
                     /translation="MTDASVVAVGTPQSFRQQVARAVEMGADEVGWVQSATAAEELLI
                     NAYQPIDVLVLSPEVKEPDALGLAEFVGRQAPGTAMVLVRDHTWNGLLPAAMRAGIRD
                     VVDMTQGTEELRDAVERAIVWAGNLRSVNVTATDGQPVTRGRILSIFSSKGGTGKTFL
                     TSNLATSISEVTGQDTAVVDLDIDMGDVFTYFGREPTATIQDLMELGEGAERSRIKQI
                     GVQLSEHLWGFGAPPDPAAEAPAGEAIGKFLRALRNEFGWVVVDASVDYSDSALVCFD
                     LSDAICLVTGLDVVGVKHLSKSLDTLLTIGLPRERFRVVLNRADSKVGLDASDVERVM
                     KINVDAMIPSSRLVPTSLNKGRPVVLDEPNSEVAQAVRQLAVRFAGQAEMVAAGEAGI
                     DTSNKKKRKLFARS"
     gene            6757..8073
                     /locus_tag="VF036_01655"
     CDS             6757..8073
                     /locus_tag="VF036_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX6263966.1"
                     /translation="MSLADRLQKAQQKDRIAEVRIRVQERLVESLGPRLYDATLSDTE
                     LEGMVHQRLRELLDEEEGPLAAQEKLLIVRQIGDSVLGLGPLEPYIRDPEVTEVMVNN
                     WDTIYVERSGKLFWTGTKFHDEGQLRRTIDKIVAKVGRRVDEASPMVDARLPDGSRVN
                     AIIPPLAIDGPTLTIRKFAADPYQADDLVEFGTMSSAVSKFLESCVRGRINVMVAGGT
                     GAGKTTTLNVLSSFIPDDERIVTIEDAAELKLQQPHVVRLEARPPNIEGKGQVSIRDL
                     VRNSLRMRPDRIVVGEVRGAEALDMLQAMNTGHDGSISTIHCNSPRDALSRLETITMM
                     AGMDLGSRAIREQIASAIQLIVYQSRLKDGTRRFTHVTEVAGMEGEVITLQDIFLFDY
                     SAGMDDEGRFRGRLKSTGLRPKFVEKLQERGVHVDPELFALESRDR"
     gene            8076..9083
                     /locus_tag="VF036_01660"
     CDS             8076..9083
                     /locus_tag="VF036_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6263967.1"
                     /translation="MLAADTFFSSQAMLAVVVALVALAAFALGWLLLGTAARVKADRD
                     MAARMAAIGRPVPQQTASAVGKPGTGWIPESVSSFGERFAKAQGFGEKLDAQLEAAGV
                     SLRSGEFVVATIGAAILGGVLGAALFQNLLLAGVIGLIGGALPTVLLRMSLGRRGDKM
                     REQLPDVLTIMASSLRAGHSFLQALDTVAREIPAPADVEFQRLVAEIRLGRPPEDALE
                     SLADRVGSADFRWAVLAVNIQREVGGNLAEILDNVADTLRERAMMRRQIRVLTAEGRL
                     SAWVLAILPFGIALYMFAVNPEYISLLFTKQVGLIMLGTGGVLMVLGILWMRKIVDID
                     V"
     gene            9076..>9881
                     /locus_tag="VF036_01665"
     CDS             9076..>9881
                     /locus_tag="VF036_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6263968.1"
                     /translation="MSEIWLFLAVGCLFLAIVLIGVFIDQQQAEKRRAVRMLETQVAG
                     PAGQSTQTVDLREQEMNENFGTRVMVPVVSRAGRIAKKVTPLDTRDRIAKKLVLAGQP
                     VGWDAERVLAFKVIGAAVGFVGSIIVLRLIDLSPLLQIVVIGLLTFVGFILPDSILSS
                     RVEDRKKEVLRTLSDTLDLLTISVEAGLSLNAAMAQVVKNVPGVLSSEFARMLQEIQL
                     GVPRSEAFRHLAERTDVEELNGFALAMIQADIFGVSIASVLRTQAQQLRI"
BASE COUNT         1684 a         3222 c         3415 g         1560 t
ORIGIN      
        1 gcttcgagac tcgggatctc tcctcggacc gtggagacac acgtggcgac gctctaccga
       61 aggttgggcg tgaacacgcg ggtgcaggct gtttcccagg ctgcgagact cggattggtc
      121 gacctgtagc gttgcgaccc actgttccca atggcgaagg tcgtcccgat gatggtgagt
      181 gagtcgacga tcggcccgag tgggggaaaa actcccaccc accacgacgt ggtgatcgtc
      241 gagccgttcc cggtcgtccg cgcgggcctc ggcctgatcg tcgacgcgag tccaggactc
      301 acggtcgccg ctacgtcggc gagcgccgag gaagcgatcc ccctcatcga tcgcctcgat
      361 cgaacgcagc tcgtcgtgct ggtgtcgatg ggcttggcgg gcgagtccga cgcattctgg
      421 ttggtccgtg cgctccgcga acggttcccc gggctcgtga tcctcgggct gggtgcgaat
      481 gccgatccga acaccgtgtc acgcgcactg ttcacgggcg cggatggatt catcgacaag
      541 aacgtggagg tcgaagagtt cctcgaggcg atccgtgacg cgagcgacgc ggaggtggtg
      601 atcgccggac ccgccgcgga ttcggtggga acgatcgcgg aagggatcga acgccgcagg
      661 agccagacct tccgtctcac ccgacgcgaa cgagaggtcc tggccgtcgc ggcggaggga
      721 ctgaccgctc gggagatcgc cgaccggctg ggcgtccgcg agcggaccgt cacgacccac
      781 ctcgcacgga tctacgggaa gctcggagtc ggcaacaggc tcgctgcgct gcggctcgcg
      841 gcgaggtcgg gactcttgac gctcccaccc gccgagtagc ttcaggaccg aacgaagatc
      901 gaacctgagg ggatctgcgc gccgagcgtc caccctgggg tctgtgccag cgcctcgccg
      961 agcggctctc cggggggcag caccacgccc tggatctcgt atcgatcgag ggtccgttcc
     1021 catccccctt cggcacgggt cgctcgcgag tagtcgcgcc acacggtgct cgggaacaac
     1081 tcgatccggg aatcgaccat caccggatcc tcgggcagcg aatactcgaa ccacgaggcg
     1141 aacggctgga agaccaagag ccttgacccg gaggggaggg ccttcctggt cgcgatcacg
     1201 agatgttccg gcgcatctgc ggccagccgc gcaggaccgc ccgtcgccgg atccgtcccg
     1261 gctcgaaggc tcagggtgag tccgagggcg atcaccatcg aggtcgcgat gagcgtcgga
     1321 acccgcctcg actcaccgcc ctgcccggga tcgacgggtt cccgtgtcgc gaaccagccg
     1381 gccacgatcg gaggtgcggc gagggcccac cacagggtgc ctcggagagc cggcaccccg
     1441 agagcgaaga acacgagcag acgggcgacg tcgatgaggc ggatctcctc tcgcttcgag
     1501 acgccgacga tggccacggc gacgacggag gcccagaacg ggccgccgct gagggagaag
     1561 ggggatggtg gtctccactc tgcgaccgaa ctccggatcg tttcgttccc gaccagatcg
     1621 agggcgtacg tccagacgga gcctccgaac ggggtgacga aggtagcccc cgccgtggcg
     1681 aggagcagca gcagggtcgg ccgtggatcc ttccgttcga gcagatcctc gcagagggcg
     1741 aaggcgacca gaccgggtgc gaggacgaag ctcccgtgga cgttggccca gatcacggcg
     1801 aggaccggaa caccccacat ccagcgatcc cgcctctcca gcaagagcca ggtgccggtg
     1861 aagaggggta ccgcaagcgc ctgcggtcgg gcggcgagcg atccggaggc aaccatgaat
     1921 ccgagcagcg agaggatcgc cgccgttcgc ggcgtcgcct tcgccctcct gcagctgcga
     1981 tagagcagcc cgaatccgga cgccatggcg ccggcatacg tgatcgcaac cccagcccaa
     2041 ccgagtagcc gatgcgcccc agcgaagatc aattgagcgg cccattgctg gttcagccag
     2101 ggtcggcccc cgctcgtgaa cgtgaagggg tcgacgagga gaaggtcccc cgttcggagg
     2161 ctgagctcgc cggcgcgcac gtgataggcg aggtcgatgg cgcgcatctt cgccaggagg
     2221 gccgcgagcg cagggagagc ccaggagaga gtggcccagg gcgggtcggc ccttcggcct
     2281 acggaggcct gccgaaagtg agcacggaaa tctgcccctt tgggggtttc cacgcgggca
     2341 tcctacgcgg taccatccgg cgtcttctcg gtcgacgagg agatgcgtac gtagaaacac
     2401 gtagataact ccagtggaat acgggattcc cctggaggga tggggcgcct tatcgtgctc
     2461 gctggcggat tgactctgcc aacggggcga tgccccgagg acgggatctc ggaggagagg
     2521 aggtgtatcg gatgcttaag ctctacacgg gtcttcagac ctggtgggcc gacctcaagg
     2581 gccgcgctca ggaggagcac ggcgcgaccg cggtcgagta cgcgttgatg gtcgctctga
     2641 tcgcggtggt gatcatcctc gcggtgacct tcatcggcac gtctgccgag tcgaagttca
     2701 accaggtcgg atcggccgtc aacaacgccg gttcgtagct ccatgtgatc gagcggccgt
     2761 cggccgctct cgccgccgcc tcgaggcggc ggcgacagga gccgacggag acccaggagt
     2821 gatggatgtg agcattcgga gcaagctgca ccgtgaggag ggggccgccg cggtcgagtt
     2881 cgccttgatc gtcggcctgc tcgccgtcct catcttcggg ttgctcgaat acggcctcgc
     2941 gttctggcag gtgcagaacc tgcgcgccgc agcccgcgag ggagctcggg tggccgccgt
     3001 gcgcggcgac gccaccgagg tgcggagcca tatggtcgct tcgtccgcgg gctcactgag
     3061 cggtggatgg acgtacaccc agaatcgaac gtgcgacggg tctgacgcca acaaggggca
     3121 agaggtgacg atccagatca ccaacagctc gctgtccggg ccggtgcagg acgccttcca
     3181 ggtgtccatc cccttcctcc cgccgctcca gctgaatccc gtcttgcgag gcagcttccg
     3241 ctgcgaatga gcttcggatg aggagacgac cccccttggc tgatccccac aggctgcacg
     3301 acgacgaacg cggagcgacg gccgtgatcg tcgtgctgag cttgctggcg ctcttcggac
     3361 tggtcgtgct cacggtggac gtgggacaac tgctcttcca acgccgcggg atggtgaacg
     3421 catcggacgc tgcggcactc gccgcggcgc agacgtgcgc gggcctcgac gattccgacg
     3481 tcccggaggc ggtggccgac gcattcgcgg tgaacaacgt cggcagcgcg atcacggcgg
     3541 tcccgaacat caccgagatg gtcgggtgcg acggtccggc ctacggccac gtcagcgtcg
     3601 agtacgcgat gaaccagaac ctcttcttcg ctggggtcct gggcatcagc ggaccggcct
     3661 ccgtgcgcac cgcggccacg gccgggtggg ggccatccgg cggcgcgaac ccgctgccga
     3721 tcgtcgtcta cgtcggcaat gaccagggca actgcgacat cgaggaggga actccggcag
     3781 gtgtcgactg ctacctctgg tacgacaacg acctgttcga gaactcggcg ttcgggttcc
     3841 tgaacctgtg caccgcgacg gacccctgct cgcacgggtg ggacgtcgcg gccaacgcga
     3901 actgcccgaa cgtgggctcg agcctccgcg acgactggat cgccgggaac tggacggggg
     3961 ggccgaacga gctcaactac cccgatccca cctacgtgtg cgtcgtgacg gggctatcat
     4021 cgtcgaactg ggtggagttg gatcgacgcg tcggggacga tctgatcttc cccgtcgacg
     4081 actgtccgac gaacgtcgat aacaacggca acatcgtcgg ctgcgacgcg ttcccgaaca
     4141 agtacaacat cgtcgggttc atcaccctcc atctcgacgc ggtgctggac agtgcggcgg
     4201 agtggggcgg agtcgcccag acgcattgca gcccgaacaa cgtgaacgtg acgcccggtc
     4261 tcgtggtccc gctttcatcg ttcatgggcg ggtcgtgccc gaatggcagt acgccttcgg
     4321 gagtccttga tttcaggatc gacgatcaga cgtcgagccc gaactggatg tacgacgacc
     4381 tgaacaagca gttcacctgg acgggaccgg ccgacagggt caacgtcgat ttcgactggt
     4441 ggctcgacgg ggagtgcggt cagccgccgc cgaactcgag cgcggtctgc atcaaggtcc
     4501 acacggtgga ggtccgcttc ggtggaacgt ctccaggcgg gggagcggac ttcggtctgc
     4561 ggtccatcag gctatgcgac atgcagttcg ggagctgtcc cgaacaggat tgaggacgga
     4621 aaacactgaa gccggcaagg agtccggccg atagaggaga acgcactctg aggatgggtt
     4681 gaaaggagca agaggatgag gtcacgaggt ctggtggtag cgatagcgat cgtgctcgcg
     4741 gtgggagcgg ctgcggccgt ggtcctgtac acgcaagggg tgaaggaaga ggcggaacag
     4801 ggcgggcagc tcaccacggt gatcgtggcg acccaggaga tccccgccaa tcagcgcctc
     4861 gaccctctga tcgagcaagg tctgttcgca gagatcgacg tccctgcgga cgccctcgtc
     4921 ggcggggccg tcaccaccct caccgagctg gagggagcca ccaccacgac gccgatcctg
     4981 gcgaacgagc agatcccgac cgcccggctc tcgacctcgg aagagcagct gaacatcgtc
     5041 ggcgtgagcg aaggccacgt cggtctgtcc gtggagctcg acgctcctca gggcggcgtc
     5101 ggcaacatca atcccggcga caacgtccag gtgttcgcga cctaccaggg tgtctcgctg
     5161 atccccggca acctgcgtca gtatctgacg cagccggcgg acgcgcaggc cggcgccaag
     5221 aaggacgtgc cagacttcac cgtgacgctg gttccgaccg tgcgggtgct ccgcatccag
     5281 aaccccgagg tggacaccga gaccggacgg agcgacagct cgcggatcca gatcacgttg
     5341 gatctcttgc cgcaggacgc gcagaccctc gtcttcgcgc aggagaacgc gcggatctgg
     5401 ctcggcctcc tgccgccgaa cgaggatggg acgcagccac cggcgtcgac cgttccgatc
     5461 gagctcctgc tcggagcgaa gctcggatga ccgacgccag cgtcgtcgcc gtcgggacgc
     5521 cgcagtcgtt ccgccaacag gtcgccaggg ccgtcgagat gggcgcggac gaggtgggtt
     5581 gggtgcagtc ggccacggct gcagaggagt tgttgatcaa cgcctaccag cccatcgacg
     5641 tcctggtcct ctcgcccgag gtcaaggagc ccgacgcact cggcctcgcc gagttcgtcg
     5701 ggcgccaggc cccggggacg gccatggtgc tggtccggga ccacacctgg aacggactcc
     5761 tgccggcggc gatgcgcgcc ggcatccgcg acgtcgtcga catgacccag gggaccgagg
     5821 agctccgcga tgccgtggag cgcgcgatcg tctgggcggg gaacctccgt tcggtcaacg
     5881 tcaccgcgac cgacggccaa cccgtcacgc gcggccggat cctgtcgatc ttctcctcca
     5941 agggagggac cggcaagacg ttcctgacat ccaacctggc cacctcgatc tccgaggtca
     6001 cgggccagga cacagccgtg gtggacctcg acatcgacat gggcgacgtc ttcacgtact
     6061 tcggccggga gcccacggcc acgatccagg acctgatgga gctgggcgaa ggcgccgagc
     6121 gctcccggat caagcagatc ggcgtccagc tctcggagca cctgtggggc ttcggcgccc
     6181 cgccggaccc ggcggccgag gccccggccg gcgaggcgat cgggaagttc ctgcgcgcgc
     6241 tccgcaacga gttcggctgg gtcgtcgtgg acgcctcggt cgactactcg gattccgcgc
     6301 tggtgtgctt cgacctgtcc gacgcgatct gcctcgtgac gggtctcgac gtcgtcggtg
     6361 tgaagcacct gtcgaagtcg ctcgacaccc tgctcacgat cggcctgccg cgggagcggt
     6421 tccgcgtcgt cttgaaccgg gcggactcca aggtgggcct cgacgcctcc gacgtggagc
     6481 gcgtcatgaa gatcaacgtg gacgcgatga tcccgtcgag ccggctcgtg ccgacgtccc
     6541 tgaacaaggg tcggccggtc gtgctcgacg agcccaactc cgaggtcgcg caggcggttc
     6601 ggcaactcgc cgtccgcttc gccggccagg cggagatggt cgccgccggc gaggcgggga
     6661 tcgatacctc gaacaagaag aagcgcaagc tgttcgctcg aagctgaacg aaccctgagg
     6721 ccaagaagag acagaagaag gcagaggagg cacccgatgt ccctggcaga caggctgcag
     6781 aaggcccaac agaaggatcg gatcgccgag gtccgcatcc gggtgcagga gcgcctggtc
     6841 gagagcctcg gaccgcgtct gtacgacgcg accctgtccg acaccgagct cgaggggatg
     6901 gtgcaccagc gcctccgcga actcctcgac gaggaggagg gaccgctcgc cgcgcaggag
     6961 aagctcctga tcgtccggca gatcggtgac agcgtcctcg gtctcggccc gctcgagccc
     7021 tacatccgcg acccggaggt caccgaggtc atggtgaaca actgggacac gatctacgtc
     7081 gagcgatcgg gcaagctgtt ctggaccggc acgaagttcc acgacgaggg ccagctccgg
     7141 cgcacgatcg acaagatcgt cgccaaggtc ggccgtcgcg tggacgaggc gagcccgatg
     7201 gtggacgcgc gtctgcccga cggctcgcgt gtgaacgcga tcatcccgcc gctcgcgatc
     7261 gacggcccga cgctcacgat ccggaagttc gccgccgacc cgtaccaggc ggacgacctg
     7321 gtggagttcg gcacgatgag ctcggcggtc tcgaagttcc tggagtcctg cgtccgtggc
     7381 cgcatcaacg tgatggtggc cggcggaacg ggcgccggga agaccacgac cctgaacgtg
     7441 ctctcgtcct tcatccccga tgacgagcgc atcgtgacga tcgaggacgc ggcggagctc
     7501 aagctccagc agccgcacgt ggtccgtctc gaagcccgcc cgccgaacat cgagggcaag
     7561 gggcaggtct cgatccgcga cctcgtccgg aactccctgc ggatgcggcc cgaccgcatc
     7621 gtcgtcggtg aggtccgtgg cgcagaggcg ctggacatgc tccaggcgat gaacaccggt
     7681 cacgacggtt cgatctcgac gatccactgc aactcgcctc gcgacgccct gtcccgtctc
     7741 gaaaccatca cgatgatggc gggcatggac ctcggctccc gcgcgatccg cgagcagatc
     7801 gcatcggcga tccagttgat cgtctaccaa tcgcgcctca aggacggaac ccgccggttc
     7861 acgcacgtga ccgaggtcgc cgggatggag ggcgaggtca tcacgctcca ggacatcttc
     7921 ctgttcgact actcggccgg catggacgac gagggccgct tccggggccg cttgaagtcc
     7981 acgggtctgc ggcccaagtt cgtcgagaag ctccaggagc gcggcgtgca cgtggacccg
     8041 gagctcttcg cgctcgaatc gagggaccga taacgatgct ggccgcagac acgttcttct
     8101 cgagccaggc gatgctggcg gtcgtcgtcg cgctcgtcgc gctggcggcg ttcgccctgg
     8161 ggtggctcct gttggggacc gccgctcgcg tcaaggcgga ccgggacatg gcggcgagga
     8221 tggctgcgat cggccgcccg gtgccgcagc agaccgcctc cgcggtcggg aagccgggta
     8281 ccggctggat ccccgaaagt gtctcgtcct tcggtgagcg gttcgccaag gcgcagggct
     8341 tcggagagaa gctcgatgcc cagctcgagg ccgccggtgt cagtctgcgg tcgggtgagt
     8401 tcgtcgtcgc caccatcggg gctgcgatcc tgggcggcgt cctcggcgcg gcgctgttcc
     8461 agaacctcct cctcgcgggt gtgatcggcc tcatcggcgg cgctctgccg accgtcctcc
     8521 tccggatgtc cctggggcgt cgtggggaca agatgcgcga gcagctgccg gacgtgctga
     8581 cgatcatggc gtcgtctttg cgcgcggggc actcgttcct ccaggcgctc gacacggtcg
     8641 cccgggagat cccggccccg gccgacgtgg agttccagcg gctcgtggcc gagatccggc
     8701 tgggccgtcc accggaggac gccctcgagt cgttggccga ccgcgtcggg agcgcagact
     8761 tccgatgggc cgtcctcgcc gtgaacatcc agcgcgaggt cggcggcaac ctggcggaga
     8821 tcctcgacaa cgtggcggac acgctccggg agcgcgcgat gatgcgccgc cagatccggg
     8881 tcctcacggc cgaaggcagg ctctccgcct gggtcctggc gatcttgccg ttcgggatcg
     8941 ccctctacat gttcgccgtc aaccccgaat acatctcctt gctcttcacg aagcaggtcg
     9001 gtctgatcat gttgggcact ggcggtgtcc tcatggttct cgggatcctc tggatgagaa
     9061 agatcgtgga tatcgatgtc tgagatctgg ctgttcctcg cggtggggtg cctgttcctc
     9121 gccatcgtgc tcatcggtgt gttcatcgac cagcagcagg ccgagaagcg tcgggcggtc
     9181 cgcatgctcg agacgcaggt ggccggtccg gccgggcagt ccacgcagac ggtcgacctg
     9241 cgcgagcagg agatgaacga gaacttcggc acgcgcgtga tggtgccggt cgtctcccgc
     9301 gccgggcgga tcgccaagaa ggtgacgccg ctcgatacgc gcgaccggat cgcgaagaag
     9361 ctggtgctcg ccggtcagcc cgtgggctgg gacgcggagc gcgtcctcgc gttcaaggtg
     9421 atcggcgccg ccgtcggctt cgtcggctcg atcatcgtcc tgcgcctgat cgacctgtcg
     9481 ccgctgctcc agatcgtcgt gatcggcctg ttgacgttcg tcgggttcat cctgcccgac
     9541 agcatcctga gcagccgggt cgaggatcgg aagaaggagg tcctgcggac cctgtccgac
     9601 accctcgacc tgctcacgat cagcgtcgag gccggtctgt ccctcaacgc cgcgatggcg
     9661 caggtcgtga agaacgtccc cggtgtcctg tcgtcggagt tcgctcggat gctccaggag
     9721 atccagctgg gcgtgccgcg ctccgaggcc ttccggcacc tggccgagcg gaccgacgtc
     9781 gaggagctca acggcttcgc cctggcgatg atccaggccg acatcttcgg tgtctcgatc
     9841 gcgagcgtcc tgcgcacgca ggcgcagcag ctgcggatca a
//
LOCUS       DASUVE010000080         4153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_414109, whole genome shotgun sequence.
ACCESSION   DASUVE010000080 DASUVE010000000
VERSION     DASUVE010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414109"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..170
                     /locus_tag="VF036_01670"
     CDS             24..170
                     /locus_tag="VF036_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263969.1"
                     /translation="MLAINGFLEWFFLGLLSFLVAAVTLFGVYMAIQLFRSPGRSPRP
                     PRSL"
     gene            complement(127..831)
                     /locus_tag="VF036_01675"
     CDS             complement(127..831)
                     /locus_tag="VF036_01675"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6263970.1"
                     /translation="MNGGTLRVATWNVHGMRAGEDEIARVIRAERIDIAVLQESGPRR
                     RLRALGEALDMRVCGDPWAFPRRRIQNAVLVRRGLAGAVSHRLHRFSGGSLLSPRGVM
                     LATVDHRVSVLSVHLGLSKIERAHHVDQLVAILEGSAGSFVVGGDLNALPGDPGPSSL
                     AALAPDCWEAAGEGSGATYVSHAPTARIDYLFAGGTFRPLRAWTAGSTASDHLMVVAE
                     LALTESGEDGASVRDS"
     gene            complement(828..1766)
                     /gene="arcC"
                     /locus_tag="VF036_01680"
     CDS             complement(828..1766)
                     /gene="arcC"
                     /locus_tag="VF036_01680"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX6263971.1"
                     /translation="MERVVVALGGNALLRRGADDTFEELYRAARAAAERIAEIVAEGW
                     EVVVTHGNGPQVGRILIQQEAAAKTVSPMPLDVCGAESQGLIGYLLQVTIGDVFFERG
                     MERPVATVLTLTRVRPQDPAFRKPSKFIGPFYTAARARKLEEQRHWVMKEDPHGGWRR
                     VVPSPRPYSIVETPIVKRLVADGVIVIAGGGGGVPVIEKGPRLIGKEGVVDKDLAAAI
                     LAAEVEASTLLILTDVRKVQRGFGTLYPDTIDRMTADEATDLLKRGEFGAGSMGPKVQ
                     AAIDFLEAGGARVMIGDLDEAPAVLRGEAGTEIVPS"
     gene            complement(1811..3121)
                     /locus_tag="VF036_01685"
     CDS             complement(1811..3121)
                     /locus_tag="VF036_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HEX6263972.1"
                     /translation="MPRRVLIMGAAGRDFHNFNVVFRDDPSTEVVAFTATQIPYIDDR
                     TYPAKLAGERYPDGIRIHEEDELPRLIRQLDVDDVVFAYSDVSHEYVMHKASETLAAG
                     ANFLLLGPDATMLKPAIPTVAVTAVRTGVGKSQTTRAVAAALKEAGKKVVAVRHPMPY
                     GDLTAQRVQRFAELEDLDRHHCTIEEREEYEPHITTGTVIYAGVDYAAILEQAQAEGD
                     VLLWDGGNNDLPFYLPDVHIALVDPLRVGHETRYHPGEANLRRADVILINKMDSSTPE
                     QVAQLEETIAQTNPMAAVVKANSKVTVDDPDAIAGKRVLVIEDGPTLTHGEMKIGAGV
                     VAAERSGAAEIVDPRPWAVGTIAETFEKYDVGAVLPAMGYSQGQLDEMAKIIDAADAD
                     LVVIGTPIDLRHVIDIRKPAVRVRYDLDVLPGSPQLLDVLRPVL"
     gene            3242..>4153
                     /locus_tag="VF036_01690"
     CDS             3242..>4153
                     /locus_tag="VF036_01690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6263973.1"
                     /translation="MEEERPETGSPVGRVLADVRTSFAALGRSFGSPDIAKALAAYLA
                     FTITEWAAFIALIVYAYEDGGSVMVGLVSLLQLIPAALIAPVGSVLGDRHRRERVLLY
                     SYASLSATTTLAAVALLTDAPAPLVYASATTAGWMITLVRPTHASLLPRLARTPEELS
                     SAYAASSLLESVSILLGPLLAGALMRLAPGSLSGSGLVDAALAAMLLLGTVSVAAIRV
                     RTEPAKDGGTSGVRVVASDAVEGVRAVARDRRSLLLVATMGLATVQLGFVDVLIVVLA
                     FDVVGTGDAGVGFLTASIGVGAVVGAML"
BASE COUNT          571 a         1478 c         1414 g          690 t
ORIGIN      
        1 acccccgccg gtaggatgca gacgtgctgg cgatcaacgg gttcctcgaa tggttcttcc
       61 tcggactgct gtccttcctg gtggcggccg tcacgctgtt cggggtgtac atggcgatcc
      121 agctcttcag gagtcccgga cgctcgcccc gtcctccccg ctctctgtga gcgcgagctc
      181 cgctaccacc atcagatggt cggaggcggt actccccgcg gtccaggccc ggagcggccg
      241 gaacgttccc cccgcgaaca gataatcgat ccgcgccgtg ggagcgtgcg agacgtacgt
      301 cgcgccggag ccctcgcccg cggcctccca gcagtccggg gcgagcgcgg cgagcgacga
      361 cggacccgga tcacccggga gggcgttcag gtctcccccc accacgaagc tgccggccga
      421 tccctcgagg atcgccacca gctgatcgac gtggtgtgca cgttcgatct tgctcagacc
      481 gaggtgcacg gacagcacgg agacgcggtg gtccacggtg gcgagcatca cgcctcgggg
      541 agacaggagc gacccccccg agaaccgatg gagacggtgg gagacggcgc cggccagccc
      601 tcgccgaacg aggaccgcgt tctggatcct gcgccgtggg aaggcccacg ggtcgccgca
      661 cacccgcatg tcgagggcct cgccgagggc ccggaggcgc cggcgaggac cggactcctg
      721 cagcaccgcg atgtcgatcc tctcggcccg gatcacccgg gcgatctcgt cctcgccggc
      781 ccgcatcccg tggacgttcc aggtggcgac gcggagcgtg ccgccgttca cgacgggacg
      841 atctcggtgc ccgcctcgcc gcggagcacc gccggcgcct cgtcgaggtc accgatcatc
      901 acgcgggccc cgccggcctc gaggaagtcg atcgcggcct ggaccttcgg tcccatcgag
      961 ccggcgccga actcgccgcg cttcagcagg tccgtggcct cgtcggcggt catgcgatcg
     1021 atcgtgtccg gatagagcgt gccgaaccct cgttgcacct tccgcacgtc ggtgaggatc
     1081 aacagtgtgc tggcctccac ctcggcggcg aggatcgcgg ccgccagatc cttgtccacc
     1141 acgccctcct tgccgatcag ccgcggcccc ttctcgatca cgggcacccc tcccccgcct
     1201 ccggcgatca cgatcacccc gtcggcgacc agtcgcttca cgatcggcgt ctcgacgatc
     1261 gagtagggac gcggggacgg cacgacccga cgccagccac cgtgcgggtc ctccttcatc
     1321 acccagtggc gttgctcctc cagcttgcga gcccgggcgg cggtgtagaa gggaccgatg
     1381 aacttcgacg gcttccggaa cgccgggtcc tgcggccgga cccgtgtgag cgtgagcacc
     1441 gtcgcgaccg gacgctccat gcctcgctcg aagaagacgt cgccgatcgt cacctggagc
     1501 aggtagccga tcagcccctg actctcggcg ccacagacgt ccagaggcat cggggacacg
     1561 gtcttggccg ccgcctcctg ctggatcagg atgcgcccca cctgcgggcc gttgccgtgc
     1621 gtgacgacga cctcccaccc ctcggcgacg atctcggcga tgcgctccgc ggcggcgcgg
     1681 gccgctcggt agagctcctc gaacgtgtcg tccgcgccgc gtcggagcag cgcgttgccg
     1741 ccgagcgcga cgacgacgcg ttccatgttc ggcggctccc ttcaccggct ccgctcgggg
     1801 ccggagtctg ctacaacacc ggccgcagca cgtcgagcag ctgcggcgat cccggaagca
     1861 cgtcgaggtc ataccgcacc cgaacggcgg gcttgcggat gtcgatcaca tgacggaggt
     1921 cgatgggcgt cccgatcacc acgaggtcgg cgtccgccgc gtcgatgatc ttcgccatct
     1981 cgtcgagctg accctggctg tagcccatcg ccggcaggac ggccccgacg tcgtacttct
     2041 cgaacgtctc ggcgatcgtc cccaccgccc acggtcgcgg atccacgatc tcggcggcgc
     2101 cggagcgttc cgccgccacg acgccggcgc cgatcttcat ctccccgtgt gtgagcgtcg
     2161 ggccgtcctc gatcacgagc acccgcttcc cggcgatcgc atcgggatcg tccaccgtca
     2221 ccttcgagtt cgccttcacc acggcggcca tcgggttcgt ctgcgcgatc gtctcctcga
     2281 gttgcgcgac ctgctccggc gtcgaggaat ccatcttgtt gatcaggatc acgtcggcgc
     2341 gacggaggtt cgcttcgccg gggtggtacc ttgtctcgtg gccgacccgg agcggatcga
     2401 cgagcgcgat gtggacatcc ggcaggtaga acgggaggtc gttgttgccc ccatcccaca
     2461 ggagcacgtc gccctcggcc tgtgcctgct cgaggatcgc ggcgtagtcg acgcccgcgt
     2521 agatcacggt tcccgtggtg atgtgcggct cgtactcctc ccgctcctcg atcgtgcagt
     2581 gatggcgatc gagatcctcg agctccgcga accgctggac gcgctgcgcc gtgagatcgc
     2641 cgtacggcat cggatggcgc acggcgacga ccttcttgcc ggcctccttc agagcggcgg
     2701 cgaccgcgcg ggtggtctgc gacttcccga cgccggtgcg gacggccgtc acggccacgg
     2761 tggggatggc cggcttgagc atcgtggcgt ccggacccag cagcaggaag ttcgccccgg
     2821 ccgcgagcgt ctcgctcgcc ttgtgcatca cgtactcgtg tgacacatcg gagtaggcga
     2881 acaccacgtc gtccacatcg agctggcgga tcaggcgtgg gagctcgtcc tcttcgtgga
     2941 tccggatccc gtcggggtag cgctccccgg cgagcttcgc gggataggtc cggtcgtcga
     3001 tatacgggat ctgcgtggcg gtgaacgcga cgacctcggt ggagggatcg tcgcggaaca
     3061 cgacgttgaa attgtggaag tcgcggccgg cggctcccat gatcaggaca cgacgcggca
     3121 tggccgaggt cctttcagga gaggtggtgt gatgcgcggg caggagcgcg aagcgcccgc
     3181 tcttcgggag cgactgtagc ccaacacggg tcctggcgac ctggcctaga ctcctgccct
     3241 cgtggaggag gaacggccgg agaccggatc accggtgggg cgcgtcctcg ccgacgtgcg
     3301 gacgtccttc gcagcgctcg gtcgctcgtt cgggtcgccc gatatcgcga aggcgctggc
     3361 ggcctacctc gcgttcacga tcaccgaatg ggcggcgttc atcgcgctga tcgtctacgc
     3421 gtacgaggac ggcggcagcg tcatggtcgg tctggtgagt ctgttgcaac tgatcccggc
     3481 cgcgctgatc gcaccggtcg gatccgtcct cggtgatcgg caccgccgtg agcgggtgtt
     3541 gctgtactcc tatgcctcgc tcagcgcgac gacgacgctg gccgccgtcg cactcctcac
     3601 cgacgcgccg gcgccgctcg tctacgcgtc ggccacgacc gcggggtgga tgatcacgct
     3661 cgtccgcccc acgcacgcct ccctcctgcc gcggctggct cggacgccgg aggagctctc
     3721 gagcgcgtac gccgcgagca gcctcctgga gagcgtcagc atcctcctcg gaccactgct
     3781 cgccggcgca ctgatgcgtc tcgcgccggg ttccctgtcg ggatccgggt tggtggatgc
     3841 ggccctcgcc gcgatgttgc tcctgggcac cgtgagcgtt gccgcgatcc gggtccggac
     3901 ggagcctgcc aaggatggag ggacgtccgg cgtccgggtc gtcgcctccg atgccgtcga
     3961 gggtgtccgg gcggtggcgc gcgatcgccg gtcgttgctc ctggtggcga cgatgggcct
     4021 cgcgacggtc cagctcgggt tcgtcgacgt gttgatcgtg gtcctcgcct tcgacgtcgt
     4081 cggcacgggc gacgccggcg tcggcttcct gaccgcctcg atcggcgtcg gcgccgtcgt
     4141 gggagcgatg ctc
//
LOCUS       DASUVE010000081         7490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1544668, whole genome shotgun sequence.
ACCESSION   DASUVE010000081 DASUVE010000000
VERSION     DASUVE010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544668"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VF036_01695"
     CDS             complement(<1..617)
                     /locus_tag="VF036_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6263974.1"
                     /translation="MIDTPPVAAAAFAPLGLFEHRTGRAVVGRPAEIAASDDAIGVAE
                     GGRLSALTVEGEPGIGKTRLLLAARESAQARGFLTVAVGADEELRGPFLLARSIVGAP
                     EVIEAMSGSSSERAILAASSALGGRDDPAFAGMSADQRLLRSFDLATMAVRALTAERP
                     LALLIDDMQWADEDSLRLLRYVIRTEGASPIFMMFSIRPEELASLT"
     gene            1019..1453
                     /locus_tag="VF036_01700"
     CDS             1019..1453
                     /locus_tag="VF036_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263975.1"
                     /translation="MRTLRAITPALLLALLLTGAAACTSSNGTATVSLSPTLEVLQGA
                     YVYRAKGIEATFELSGGSGTLSVFNGTGFPFDDPGVYLLDARDGSRVDAEVIDAAPVP
                     DGQTREFQVEIQGAPRAKHVGLVALLEGDADLGAFFPAKDQA"
     gene            1450..3108
                     /locus_tag="VF036_01705"
     CDS             1450..3108
                     /locus_tag="VF036_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6263976.1"
                     /translation="MSRAGSAIAVVLALTALVVQDTTAFSQEDAQEQRLIDTVCAIPP
                     VLLERTLHGHRDDRAGEIQLLTREPDFVGHAGLPHSGPWDYVGQVPLFWYGPGYVPAT
                     GPVDRRVTLADLAPTQAELLGFDFDAPDGEPLLDALVPREDRPEPPALLIVLVWDGAG
                     NNVLAEHPNAWPNLRALMDEGVWYTRAEVGSSPPSTAQIHATIGTGAFSATHGVIAHH
                     FRIGDRIVTPWETGPRYLMRPTLADLYDRALGNRPLIGVSATVAIQLGMVGHGSLWGG
                     GDRDLVVLREREGAATLGAEGIRWNLPSTVAPFYHLPGYANDLPPLSTYVDDVDRADG
                     RFDGKWRDVEIESEAVHFGLQTPARIPFQDRLIEELISREGFGDDDVTDLFFINHKLI
                     DEVGHVYSMNSVEMEDSVRAEDAALPGLIELLNREVGEGRWVMALTADHGHTPDPEVS
                     GADVISPTDVGRQIAEEFDRDGDDVSVLEYTQPTEVFINERELAEEGHTLADVAEYLM
                     GLTKGEVGGEIWPVPEEHRAEPAFLAAYPSELLDRLPCLPPGEG"
     gene            3121..4455
                     /locus_tag="VF036_01710"
     CDS             3121..4455
                     /locus_tag="VF036_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263977.1"
                     /translation="MTRRATILLVVAAIVAAPSIVLQATCPVESCARSGPVRTPFCSL
                     PEDLRTKIAAGYREGRSPDVLGVTGGTGVVGSVPAVGPPTSIGSWPSTSPPTDTRTPI
                     VFWGDGVRGASSLPAGAELRQLAPTIAEILRFERPFPEVRSGQAIADLAGPDVPRLVL
                     EIAWKGVGTADLESDPDAWPFLRGLVSSGAGTLDGEVGSLPLDPSAGLTTIGTGGLPA
                     ELGIAGGWFRDVGGELVAAWAAGAPTSVIATLPDDLDRAFAQRPLIGIVGSDPADRGI
                     VGDGWYDERDRDDRVTVADGRARLRAVTRLLGNGYGRDDVPDALAVVMEGSVGSLDAE
                     LRRTVRAARSATGGRVLVVMTATGGGSAPGSGQVGASDLERIVEAGIDTDEPLIQAVV
                     PGGFYLDQRAMTAAGVTGQIAQDAALAARGPGGSPIFVDAFQGFAVPFGRYC"
     gene            4455..5468
                     /locus_tag="VF036_01715"
     CDS             4455..5468
                     /locus_tag="VF036_01715"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX6263978.1"
                     /translation="MSELAATPDGRRPISLRLAALLIVGLVLLGVSIRLFGPARVDWL
                     RAALVVFGSLVIQAVPFVLVGAAAAALIEVFVPIGAFERIARLPRPLRLPAAGLAGVL
                     LPICECGSVPIARRLVHRGLDPASAFTFMLAAPVVNPVVIASTFYAFRGRDTLWTMVL
                     GRAGLGLLVAMAVGWSLGTLGKEQVLRPSDEEDRGQSLELGKPEARWRRFFVHLADDF
                     LFMGGYLLLGASVAAIVQTFLPQGILERIGGTPVVDIVLMMAIASLLSLCSESDAFIA
                     ASFTQFGPSAQVAFLVFGPMVDLKLGALYAGSFRRVVVQTIVAVAAVSTLLGSMWIGV
                     LTG"
     gene            5465..6178
                     /locus_tag="VF036_01720"
     CDS             5465..6178
                     /locus_tag="VF036_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEX6263979.1"
                     /translation="MTRRPFSQRRIAVASVLAAWSGLFWFLIVMDRVDLFLSTRTSWV
                     VPMAAGGLAAVALGLVIAGRSSRPEPVRIREAVIMTLLVAPVVLLVTLPPSTLGSFSA
                     PKKARFSGSALSTIYGRFDETSEVTMLTVAAGQTSDEGSRLLATRAGSEVDFVGFVAR
                     DASAEADELMLSRYVVTCCTADASVVQVRVVNVTPGLFAPDDWIEVRGQLYPIGREVI
                     VVASSIEEVPRPDRPYLTP"
     gene            6231..>7490
                     /locus_tag="VF036_01725"
     CDS             6231..>7490
                     /locus_tag="VF036_01725"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6263980.1"
                     /translation="MPTMQRVLRDAAALAFAILVAAACVQAGTGQPPSASPDGDPVSP
                     EPTHAAPPQPNPADGVLGAPDLRDEPDPDAVPADGQIDVSSLLDPPTLQPPPDRSRPF
                     DPERGIFNLDHLVFIVLENRSFDHYFGTFPGADGLPRDANGRIDVCQPDPATGVCHRP
                     FHDTNFFDQAGPHGELGSHISIAGGEMDGFVKALRKIGNGCMKPHNADFPPCVMATPG
                     PQGQPDVMGYHTGKELPIYWKYARTFTLHDRMFAPTDSWTLPAHLFLISAWSATCTDL
                     DDPMSCSSDQHNPGSTPTNPTSRWSPQQGAPRPYIWAPITWLLYRAGVDWRYYVGEGT
                     CIAPPCVELKGTMTAAVQNPLPGFQATVATGQLDHVRSNEEFMRDARSGNLPPVSWVM
                     PVVDRGEHPPDSIELGQAYVARLINSVM"
BASE COUNT         1057 a         2600 c         2648 g         1185 t
ORIGIN      
        1 gtgagcgagg cgagctcctc cggccggatc gagaacatca tgaagatcgg gctcgccccc
       61 tcggtcctga tgacgtaccg gaggagacgc aggctgtcct cgtccgccca ttgcatgtcg
      121 tcgatcaaca gcgcgagcgg ccgttcggcc gtgagagcac gaacggccat ggtcgcgagg
      181 tcgaacgagc ggagcagccg ctggtcggcg gacatgcctg cgaacgcggg gtcgtcacgc
      241 ccgccgagcg ccgagctcgc cgcgaggatc gcccgctccg acgaggagcc gctcatcgcc
      301 tcgatcacct ccggcgcgcc cacgatcgag cgggcgagga ggaaggggcc gcggagctcc
      361 tcgtccgcgc ccaccgccac cgtgaggaac ccgcgggcct gggcgctctc ccgcgccgcc
      421 aggaggaggc gcgtcttgcc gatcccgggc tcgccctcga cggtcagcgc gctcaagcgt
      481 ccgccttcgg caacgccgat cgcgtcatcg ctcgcggcga tctcggccgg tcgcccgacc
      541 acggcgcggc cggtgcgatg ctcgaagaga cccagcgggg cgaacgcggc cgcggcgacg
      601 ggaggtgtat cgatcatgag cgcccctcga agggtgaacg gtcgcgttcg gtcatccggc
      661 gattatccgg cgcgaggggg ctcactgcta tgctggagac cccgagcgat cggggagatc
      721 cggcatcgag cgcctctggt ggcatgccct gacccacgtg cccgccctac ggatcccggc
      781 gaaccgcgac gagaggcagg ccgcatggaa gggatcagcc acaaggaccg cgttttcggt
      841 tcccccgcac gaggcaagaa ggcagccgat cggccgccgc gaggacggat gtgcgtccac
      901 atcgggtgcg agacggtcct gtccacctac aacccatccg atctgtgctg gctccacgcc
      961 ccgcccgcct atcgccacgc cctgagtcgc gactagaccg ttgatagcat ccggccggat
     1021 gcgaacgctc cgggcgatca cgcctgcgct gttgctcgcc ctgctgctca cgggagccgc
     1081 agcgtgcacg agctcgaacg gtacggccac cgtctccctg tctccgaccc tggaggtcct
     1141 ccagggtgcg tacgtctacc gagccaaggg gatcgaagcc acgttcgagc tcagcggcgg
     1201 atcggggacg ctctccgtct tcaacgggac cggcttcccc ttcgacgatc ccggggtgta
     1261 cctgctggac gcgcgcgacg gctctcgggt cgacgccgag gtgatcgacg ccgctcccgt
     1321 ccccgacgga cagacgcgcg agttccaggt cgagatccaa ggcgctccca gagcgaagca
     1381 cgtcggcctg gtcgccctgc tcgagggcga cgccgatctg ggcgcgttct tcccggcgaa
     1441 ggaccaggca tgagtcgggc ggggtccgcg atcgctgtgg tgctcgcact caccgcgctc
     1501 gtcgtccagg acacgacggc cttctcacag gaagacgccc aggagcaacg cttgatcgat
     1561 accgtctgcg cgatcccgcc ggtgctgttg gagcgcacgc tccacggcca ccgtgacgat
     1621 cgcgccgggg agatccagct cctgacacgg gaacccgact tcgtcggtca cgctggcctg
     1681 ccccactcgg ggccgtggga ctacgttggc caggtgccgc tgttctggta cggccccggc
     1741 tacgtgcccg cgaccggacc ggtcgaccgg cgggtcacgc tggccgacct ggcccccacc
     1801 caggcggagc tgctggggtt cgacttcgac gcccccgacg gcgagccgtt gctcgacgcg
     1861 ctggtccccc gagaggaccg tccggagccg ccggcgttgc tcatcgtgct cgtgtgggac
     1921 ggggccggga acaatgtgtt ggcggagcac cccaacgcgt ggcccaacct ccgagcgttg
     1981 atggacgaag gcgtctggta cacgcgcgcg gaggtggggt cgtcgccccc gagcaccgcg
     2041 cagatccacg ccacgatcgg gaccggtgcc ttctccgcga cccacggggt gatcgcgcac
     2101 cacttccgga tcggcgatcg gatcgtgacc ccgtgggaga ccggcccccg ctacctgatg
     2161 cggcccacgc tcgccgatct ctacgaccgt gccctcggga accgcccgct gatcggcgtg
     2221 tcggcgacgg tcgcgatcca gctcgggatg gtcggtcacg gctcgttgtg gggcggcggc
     2281 gaccgcgacc tggtggtgct ccgggagcgg gagggagccg ccacgctcgg cgccgaaggc
     2341 atccgctgga atctcccctc gaccgtggca cccttctacc acctgcccgg atacgcgaac
     2401 gacctgccgc cgctgtccac ctacgtcgac gacgtcgacc gcgcggacgg acggttcgac
     2461 gggaagtggc gcgacgtgga gatcgagagc gaggccgtgc atttcgggtt gcagacaccg
     2521 gcccggattc cgttccagga tcggcttatc gaggagttga tctcgaggga agggttcgga
     2581 gacgacgacg tgaccgatct gttcttcatc aaccacaagc tgatcgacga ggtcggccac
     2641 gtgtattcga tgaacagcgt ggagatggag gactcggtcc gggccgagga cgccgccctc
     2701 cccgggctga tcgagttgtt gaaccgcgag gtcggcgagg gtcgatgggt catggcactc
     2761 acggccgacc acggacacac gcccgacccg gaggtgagcg gtgcggacgt gatctcaccg
     2821 accgacgtgg ggaggcagat cgcggaggag ttcgatcgcg acggggacga cgtgtcggtg
     2881 ctcgagtaca cgcagccgac ggaggtcttc atcaacgagc gcgagctggc ggaagaaggg
     2941 cacacgctgg ccgacgtggc cgagtacctg atgggactca cgaagggcga agtcggcgga
     3001 gagatctggc cggtcccgga ggagcatcgg gcggagccgg cgttcctcgc ggcgtaccca
     3061 tcggaactgc tcgaccgtct cccgtgcctc cctcccggtg agggctgagc cgagcatgcg
     3121 atgacccggc gggccacgat cctgctggtg gtggcagcga tcgtcgccgc gccgagcatc
     3181 gtgctccagg cgacctgccc cgttgagtcg tgcgcacggt ccgggccggt gcgcacgccg
     3241 ttctgctccc tcccggagga tctccggacg aagatcgcgg cggggtatcg cgagggaagg
     3301 tcgccggacg tcctcggcgt caccggaggg accggggtcg tcggctcggt gccggcggtc
     3361 ggcccaccga cctcgatcgg gagctggccg tccacgtcgc cgccgacgga cacgaggacc
     3421 cccatcgtgt tctggggtga cggcgtacgc ggagcctcga gcctccccgc cggcgcggag
     3481 ctgcgccagc tcgcgccgac gatcgcggag atcctccggt tcgagcgacc ctttcccgag
     3541 gttcgttccg ggcaggcgat cgcggacctc gccggacccg acgtccctcg gctcgttctg
     3601 gagatcgcct ggaagggggt cggaacggcc gacctggaga gcgatcccga tgcctggccc
     3661 ttccttcgtg ggctggtgtc gtcgggagcc ggaacgctcg acggagaggt cggttcgctc
     3721 ccgctggacc cctcggcagg gttgacgacg atcgggacgg gaggcctccc cgcggagctc
     3781 ggcatcgcgg gcggctggtt cagggacgtc ggcggggagt tggtcgcggc ctgggccgcc
     3841 ggcgctccga ccagtgtgat cgcgacgctt cccgacgacc tggaccgcgc gttcgcgcag
     3901 cgtcctctga tcgggatcgt cggctcggat cccgccgatc gcgggatcgt cggcgacggc
     3961 tggtacgacg agcgcgatcg ggacgatcgg gtgacggtcg ccgacggtcg agcccgcctg
     4021 cgggctgtca cccgactgct cgggaacggc tatgggcggg acgacgtacc ggacgccctc
     4081 gcggtggtga tggaggggag cgtgggctcg ctcgacgcgg agctgcggcg caccgtgcga
     4141 gccgcacggt ccgccacagg cgggcgggtc ctggtcgtga tgaccgccac cggcggcggg
     4201 agcgctcccg gatcgggaca ggtcggcgcg tcggacctcg agcggatcgt cgaggccggg
     4261 atcgacacgg acgagccact gatccaggcc gtcgtgccag gcggcttcta cctcgaccag
     4321 cgagcgatga cggcggccgg ggtcacgggc cagatcgccc aggatgccgc cctggcggct
     4381 cgtgggccgg gcggatcgcc gatcttcgtc gacgccttcc aaggcttcgc cgtccccttc
     4441 gggaggtact gctgatgtcg gagctcgccg ccacaccgga cggtcgacgg cccatctcac
     4501 tccggctcgc ggccctgctg atcgtcggcc tggtcctgct cggggtgtcg atacggctgt
     4561 tcgggcccgc acgggtggac tggctccggg ccgcgctcgt cgtcttcggg tcgctcgtga
     4621 tccaggcggt tccgttcgtg ctcgtcgggg ctgccgccgc cgcgctgatc gaggtcttcg
     4681 tcccgatcgg agctttcgag cggatcgcca ggctgccccg gccgcttcgg ctccccgctg
     4741 cgggcctggc gggcgtcctc ctcccgatct gtgagtgcgg atccgtgccg atcgcccgac
     4801 ggctcgtcca tcggggcctc gacccggcgt ccgcgttcac gttcatgctg gcggcgccgg
     4861 tggtgaaccc ggtcgtgatc gcgtcgacct tctatgcgtt ccgaggccgg gacacgctct
     4921 ggaccatggt gctcggtcgc gccggactcg gcttgctcgt cgcgatggcc gtcgggtggt
     4981 cgttggggac cctggggaag gagcaggtcc tgcggccgag cgatgaggag gatcgaggcc
     5041 agagcctcga gctcgggaag cccgaggcgc ggtggcgaag gttcttcgtc cacctcgccg
     5101 acgacttcct cttcatgggc ggctacctgc tgctcggcgc ctcggtggcc gcgatcgtgc
     5161 agacgttcct cccgcagggg atcctcgagc ggatcggagg aacgccggtg gtcgacatcg
     5221 tgctgatgat ggcgatcgcc tccctgctgt cgctctgctc ggagtccgac gccttcatcg
     5281 ccgcctcgtt cacacagttc ggcccgtccg cgcaggtcgc gttcctcgtg ttcgggccga
     5341 tggtcgatct caagctcggc gcgctctacg cggggagctt ccggcgggtg gtcgtgcaga
     5401 cgatcgtcgc ggtggccgcc gtgagcacgc tcctcggctc gatgtggatc ggggtgctga
     5461 ccggatgacc cgccggccct tcagccagcg tcggatcgcc gtggcctcgg tgctcgcggc
     5521 gtggtcggga ctcttctggt tcctcatcgt catggaccgg gtcgacctct tcctgtccac
     5581 gaggacctct tgggtggtcc cgatggccgc gggcggactg gccgccgtgg cgctcgggtt
     5641 ggtcatcgcg gggcgttcct cgaggccgga gcccgtccgg atccgggagg cggtcatcat
     5701 gacgctcctg gtggcgccgg tcgtcctgct ggtcacgctc cctccgtcga cgctcggcag
     5761 cttctcagcg ccgaagaagg cacggttctc cggctccgca ctgtcgacga tctatggccg
     5821 gttcgacgag acgagcgagg tcacgatgct gaccgtcgcc gcaggtcaga cgtcggacga
     5881 ggggtcccgc ttgctcgcga cccgtgccgg ttccgaggtc gacttcgtcg ggttcgtcgc
     5941 gcgtgacgcg agcgctgagg ccgacgagct gatgctgtcc cgctacgtcg tgacctgctg
     6001 cacggccgac gccagcgtgg tgcaggtacg cgtcgtgaac gtcacgccgg ggctgttcgc
     6061 accggacgac tggatcgagg tcagggggca gctctatccg atcggtcgcg aggtcatcgt
     6121 ggtcgcgagc tcgatcgagg aagtccctcg acccgatcgg ccgtacctca cgccgtaggg
     6181 atccggccgg cggcgaaggc gatccgtacc caccgatagg atgcctggcc atgcccacca
     6241 tgcagcgcgt cctgcgcgac gcggctgctc tcgcgttcgc catcctggtc gccgcagcgt
     6301 gcgtgcaggc gggcaccggt cagccaccct ccgcgtcccc ggacggagat ccggtgtcgc
     6361 ccgagccgac gcacgcggca ccgccgcagc cgaacccggc cgacggggtc ctgggtgcac
     6421 cggatctccg cgacgagccc gaccccgacg cggtgcccgc ggacggccag atcgacgtgt
     6481 cgtcactcct ggatccgccg acgctgcagc cgccgccgga ccggtcgcgg cccttcgacc
     6541 ccgaacgcgg gatcttcaac ctcgaccacc tcgtcttcat cgtgctggag aatcgctcct
     6601 tcgatcacta cttcggcacg ttcccgggtg ccgacgggct cccgcgagac gcgaacggtc
     6661 ggatcgacgt ctgtcaaccg gatcccgcga cgggcgtgtg ccaccgcccc ttccacgaca
     6721 ccaacttctt cgatcaggcc ggcccgcacg gcgaattggg atcgcacatc tcgatcgccg
     6781 gtggggagat ggacggattc gtcaaggcgc tgcggaagat cggcaacgga tgcatgaagc
     6841 cgcacaacgc cgacttcccc ccgtgcgtca tggcgacgcc cgggccgcag gggcagcccg
     6901 acgtgatggg ctaccacacg gggaaggagc tcccgatcta ctggaagtac gcgcggacct
     6961 tcaccctgca cgaccggatg ttcgcgccga ccgattcctg gaccctgccg gcgcatctat
     7021 tcctgatctc cgcgtggtcg gcgacctgca cggacctgga tgacccgatg agctgctcgt
     7081 cggaccagca caaccccggg agcactccga ccaacccgac ctcacggtgg agcccgcagc
     7141 agggcgcccc tcgcccgtac atctgggcgc cgatcacgtg gctgctgtac cgcgcgggcg
     7201 tcgactggcg ctactacgtg ggcgagggca cctgcatagc tcctccgtgt gtcgagctga
     7261 aggggaccat gaccgcggcg gtccagaatc cactgccggg cttccaggcc acggtggcga
     7321 cgggccagct cgaccacgtc cgctcgaacg aggagttcat gcgggacgcg cgctccggca
     7381 acctcccacc ggtgtcgtgg gtgatgccgg tcgtcgatcg tggcgagcat ccgccggact
     7441 cgatcgagct ggggcaggcc tacgtcgcgc gtctgatcaa ctccgtgatg
//
LOCUS       DASUVE010000082         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_602775, whole genome shotgun sequence.
ACCESSION   DASUVE010000082 DASUVE010000000
VERSION     DASUVE010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602775"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="VF036_01730"
     CDS             <1..1270
                     /locus_tag="VF036_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX6263981.1"
                     /translation="ARISHPNLVAVFDAGSEGDVHFIVMEHVAGETLTDVLRREGRIA
                     PGQAAGIAASVGDALAVAHAARIVHRDVKPGNVMLTSEGRLKLMDLGIARGLDGEDIT
                     RTSAILGTAGYVSPEQAHGEPVDHRSDIYSLGCVLYELLTGRQPFEADNPVAVAYQHV
                     HDAPTPPSALEPSVPQALEAVVLRAMEKDPAARYQSVEEMTTALEERAALGSSIDATV
                     PLPVVAPGTAAAAVDIPTGRTEELPRRADRPPPRRPLVPLMIAAVALALLGGLALALF
                     GGEDPPDGATLSPTRSSSLSPSPSTSPSPSPSASPSRSASASPTPPPVDPLQGAVVAL
                     QDVMAEGVAEGRISESAAQEIENGLEESLERFADGEVEEAIHTLEDLEGTVDELLKQQ
                     EIHQSQEQRIDGAIEDVARAMFDAASSEDE"
     gene            complement(1272..1889)
                     /locus_tag="VF036_01735"
     CDS             complement(1272..1889)
                     /locus_tag="VF036_01735"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEX6263982.1"
                     /translation="MRERSRIRAAVRAFGFGCLMTAAGLGGYVGWLLWGTGLETARAQ
                     EELGDDVRPLFEHPTPPTANERYLTGEAYAAIVIPSIDIDFIVVEGTDYESLKKGPGH
                     YPESAHPWDGTGQVAIAGHRTTYLHPFLNLDRVNVGDRIDLITKHGTYRYEVKRRYVL
                     PEAGSGAAIVDTKRPTLVLTTCHPKYSSRERLIVTAMLVQGPEDP"
     gene            1992..>2967
                     /locus_tag="VF036_01740"
     CDS             1992..>2967
                     /locus_tag="VF036_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX6263983.1"
                     /translation="MSVTSPLLDGLNDIQRQAVLQTEGPVLIVAGAGSGKTRALTHRI
                     AYLIRECGVSPGEILAITFTNKASREMAERVGGLLGGPISRGMWVLTFHSACARILRK
                     EHTHLGVPSNFTIYDDGDTERLIAGIARDMNVDPKRYPAKAIAAAIGKAKDRVLGPMD
                     FADLTSNLYEETVSKLYTEYERRKRDAGALDFDDLISEAVRLFRDHTEVLQHYQERFR
                     YILIDEYQDTNRAQYEFVNMLAEKYRNICVVGDADQGVYSWRGATIQNILDFERDYPD
                     AAVFLMEQNYRSTQNILTVANALIENNVQRKPKNLWTEAGSGDPVVRVR"
BASE COUNT          492 a         1020 c          998 g          457 t
ORIGIN      
        1 cgcgcggatc tcccatccga acctcgtggc cgtgttcgac gcgggctcgg agggcgacgt
       61 ccatttcatc gtgatggaac acgtcgcggg agagacgctc acggacgtgc tcaggcgaga
      121 agggcggatc gcgcccgggc aggccgcggg gatcgccgcg agcgtcggcg atgcgctggc
      181 ggtggcgcac gccgcccgga tcgtgcaccg cgacgtcaag ccggggaacg tgatgttgac
      241 gagcgagggt cgtctcaagc tgatggatct cgggatcgcc cgtggcctcg acggggagga
      301 catcacccgc acctccgcga tcctcggcac ggccggctac gtctcgccgg agcaggccca
      361 cggcgaaccg gtggaccatc gctcggacat ctactccctg ggctgtgtgc tctacgagtt
      421 gctcacgggc cggcaaccgt tcgaagccga caaccccgtg gcggtcgcct atcagcatgt
      481 gcacgatgcc ccgaccccac cctccgcgct cgagccgtcc gtgccccagg cgctcgaggc
      541 cgtggtcctg cgcgcgatgg agaaggatcc cgcggccagg tatcagagcg tcgaggagat
      601 gacgaccgcc ctggaggagc gggcggcgct cggatcgtcg atcgacgcga ccgttcccct
      661 cccggtggtc gctccgggga ccgcggctgc ggcggtcgac atccccacag ggcgaaccga
      721 ggagttgcca cggagggccg atcgaccacc gccccgtcgc cccttggtcc cgctgatgat
      781 cgcggccgtc gcgctcgcgc tcctgggcgg tctcgccctc gcgctcttcg gcggcgagga
      841 tccccccgac ggtgcaacgc tgtcgccgac ccggtcatcg agcctgtccc cttccccgtc
      901 cacgtcacca tccccatcac cctccgcatc gccgtcccgg tcagcctctg cgtccccgac
      961 cccgcccccc gtcgacccgc tccaaggagc ggtggtcgct ctccaggacg tcatggcgga
     1021 aggcgtcgcc gaaggcagga tctcggagag cgccgcccag gagatcgaga atggtttgga
     1081 ggagtcgctc gagaggttcg ccgacggcga ggtggaggag gcgatccaca cgctcgagga
     1141 cctcgagggg acggtcgacg agcttctgaa gcagcaggag atccaccagt cccaggagca
     1201 gcggatcgac ggtgcgatcg aggacgtcgc tcgggcgatg ttcgacgccg cgtcctccga
     1261 ggacgagtga cctacgggtc ctcgggtcct tgcacgagca tcgccgtcac gatgaggcgc
     1321 tcacggctcg agtacttcgg gtggcacgtc gtcaggacca gcgtgggacg tttcgtgtcg
     1381 acgatcgcag cacccgatcc cgcctcggga aggacgtacc ggcgcttcac ctcgtagcga
     1441 tacgtcccgt gcttcgtgat cagatcgatc cggtcgccga cgttcacgcg gtcgaggttc
     1501 aggaagggat ggagatacgt cgtgcggtgt cccgcgatcg caacctgacc cgtcccgtcc
     1561 cacgggtgcg cggactctgg atagtgtccg ggtcccttct tcaggctttc gtagtcggtc
     1621 ccctcgacca cgatgaagtc gatgtcgatc gaggggatca cgatcgcggc gtacgcctca
     1681 cccgtcaggt agcgctcgtt cgcggtgggc ggcgtggggt gctcgaagag cggccggaca
     1741 tcgtcgccga gctcctcctg ggcgcgggcc gtctccaggc cggtgcccca caggagccag
     1801 ccgacgtatc cgccgagacc ggcggccgtc atcaggcagc cgaacccgaa cgcccggacg
     1861 gcagcacgga tccgcgatcg ttcccgcatc cgctcacggt accccgggga tccgccccgc
     1921 ggcatctgcc gaaggtccag gtcagggcgg cgatcgtcgg acgggtcgcg agcccgcgct
     1981 agcatcgtcg cgtgagcgtc acctcccccc tcctcgacgg cctcaacgac atccaacggc
     2041 aggcggtcct ccagacggaa ggccccgtcc tgatcgtcgc cggagcgggg tcgggcaaga
     2101 ccagggcgct cacgcaccgg atcgcctacc tgatccggga atgcggggtc tctcccgggg
     2161 agatcctcgc gatcacgttc accaacaagg cctcacgcga gatggcggag cgggtcggcg
     2221 gcctcctggg cggcccgatc tcgcggggca tgtgggtcct cacgttccac tccgcctgtg
     2281 cccggatcct caggaaggag cacacccacc tgggggttcc gtcgaacttc acgatctacg
     2341 acgacggcga cacggaacgg ttgatcgccg gcatcgcgcg ggacatgaac gtcgatccca
     2401 agcggtatcc ggccaaggcg atcgcggccg cgatcgggaa ggcgaaggat cgggtgctcg
     2461 gcccgatgga cttcgccgat ctcacgtcca acctgtacga ggagacggtt tccaagctct
     2521 acaccgagta cgagcgtcgg aagcgtgatg cgggcgccct cgacttcgac gacctgatca
     2581 gcgaggccgt ccggctcttc cgcgatcata cggaggtgct ccagcactac caggagcggt
     2641 tccggtacat cctgatcgac gagtaccagg acacgaaccg cgcgcagtac gagttcgtga
     2701 acatgctcgc ggagaagtac cgcaacatct gcgtcgtcgg ggacgcggac cagggtgtct
     2761 actcatggcg aggtgccacg atccagaaca tcctggactt cgagcgggac tacccggacg
     2821 ccgccgtctt cctgatggag cagaactacc gatcgacgca gaacatcctc acggtcgcca
     2881 acgcgttgat cgagaacaac gtccagcgga agccgaagaa cctctggacc gaggccggct
     2941 ccggggatcc ggtggtgcgg gtgcgtg
//
LOCUS       DASUVE010000083         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1545524, whole genome shotgun sequence.
ACCESSION   DASUVE010000083 DASUVE010000000
VERSION     DASUVE010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545524"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1939
                     /gene="secD"
                     /locus_tag="VF036_01745"
     CDS             <1..1939
                     /gene="secD"
                     /locus_tag="VF036_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX6263984.1"
                     /translation="PDIALSGNTIEVQIPGSADATVRRRPADLFCLEGEDLIHGCSGS
                     EDDVRDVLESLEVQDQPSEVCVVDGEGEEIECFSSRAQADTFLAGVQVGPKPEPTPST
                     SVTPSPSATASPSAGPSPAASAYCLTQATGERIRCFETRAEAEDAKDALEVTVTERSY
                     CVLPAPPEPSPTPSPSASASPSASPSASASASPPPSPATFADLDLSEAEPLPCELGSR
                     EEADGALGAIEIVHEEERFCVVSSVGEQLGCSVRREEAVDLQRQSGQERLLRVIGETA
                     RLEERPVLEIVPASDPRPVTCGTQEERDLPRCAHDALEDEEVVYPGPIGQGQVAKFVL
                     GPTVITGEDFDSASAVINQSQLGTSDWTVNFELDGDGAERFSDATTAAVGAPEPTDQI
                     AIVVDRVVISSPQVQSAITGGIGEITGGFAEQEAKDLATQLNAGALPVELTRQSVRTV
                     SPTLGEESLQQGILAGLGGLILLFLYLLFYYRLLGVVAWFGMTIWAVLAVALISIAGD
                     QFGYALSLAGVAGLVISLGVTADSYIVFFERLKDEVRSGKSPRAAVQPAFARAYKTIV
                     AADIVTILAAGVLYLTAVSSVRGFALTLGVATLLDLFVVWFFKRPTVFLIAKNEKLVN
                     MRGFGLTSGVAAEVAREGAAL"
     gene            1936..>2385
                     /locus_tag="VF036_01750"
     CDS             1936..>2385
                     /locus_tag="VF036_01750"
                     /inference="COORDINATES: protein motif:HMM:NF019187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263985.1"
                     /translation="MNLHEAIETYRGHTIPHVRWVERRNIWFAISGAMVLISLVGLLF
                     GTLNFSIDFEGGARISYEFAEPVSVEDVQTTLEEHGIDDAEVQTVNDEAISIRTPSLS
                     AEGEGSAEAIRRDLAEQSGIDPVDVNIEDVGPTWGGEISRKAAIGLVI"
BASE COUNT          367 a          791 c          840 g          387 t
ORIGIN      
        1 acccgacatc gcgctgtcgg ggaacacgat cgaggtgcag atcccgggca gcgccgacgc
       61 gacggtgcgc cgacgaccag ccgatctctt ctgcctggaa ggcgaagacc tgatccacgg
      121 gtgctccggg tccgaggacg acgtccgaga cgtgctggag agcctcgagg tacaggacca
      181 gccgtccgag gtctgcgtcg tcgacgggga gggcgaggag atcgagtgct tctcgagccg
      241 ggcacaggcc gacacgttcc tcgccggcgt ccaggtcgga ccgaagccgg aaccgacgcc
      301 cagcacgtcc gtcactccga gcccgagcgc cacggcatcc ccctccgccg gcccctcgcc
      361 cgccgcgagc gcgtactgcc tgacgcaggc gaccggcgag cggatccggt gcttcgagac
      421 gcgcgcggag gccgaggacg cgaaggatgc gctcgaggtc actgtcaccg aacgttcgta
      481 ttgcgtcctc ccggccccac ccgagccctc gccgacgccg tccccctcgg cgtccgcatc
      541 tccgagtgca tctccgtcgg catccgcctc cgcgtcgccc cccccatcac cggccacgtt
      601 cgcggatctc gatctctccg aggccgagcc gctgccgtgc gaactcggct cccgcgagga
      661 ggccgacggg gcgcttggcg cgatcgagat cgtgcacgag gaggagcggt tctgcgtcgt
      721 gagctcggtg ggggagcagc tgggatgctc ggtgcgccgg gaggaagcgg tcgacctcca
      781 gcgtcagtcg ggtcaggagc gactgctccg tgtgatcggg gagacggccc gcctggagga
      841 gcgacccgtg ctcgagatcg tcccggcctc cgaccctcga cccgtgacct gcggcacgca
      901 ggaggagcgc gatctcccac gatgcgccca cgatgccctg gaggacgagg aggtcgtgta
      961 cccgggaccg atcgggcagg ggcaggtggc gaagttcgtc ctcggcccta ccgtgatcac
     1021 cggcgaggac ttcgacagcg cgtccgcggt gatcaaccag agtcagctcg ggacctccga
     1081 ctggaccgtg aacttcgagc tggacggcga cggcgccgaa cggttctcgg atgccactac
     1141 ggcggccgtc ggcgcgccgg aaccgacgga ccagatcgcg atcgtcgtgg accgggtggt
     1201 gatctcatcc ccccaggtgc agtccgccat cactggcggg atcggcgaga tcaccggcgg
     1261 cttcgcggaa caggaggcga aggacctcgc cacgcagctg aacgcgggcg cgctccccgt
     1321 ggagctgacg cggcagtcgg tgcggacggt gagcccgacg ctcggtgagg agtcgctgca
     1381 gcaggggatc ctcgccggcc tgggcggtct catcctcctg ttcctctatc tgctcttcta
     1441 ttaccggctc ctgggggtcg tcgcatggtt cgggatgacg atctgggctg tgctcgcggt
     1501 ggccctgatc tcgatcgccg gcgaccagtt cggctacgca ctctcactcg cgggtgtggc
     1561 cggcctcgtg atctcgttgg gcgtgacggc cgactcctac atcgtcttct tcgagcggtt
     1621 gaaggatgag gttcgttcgg gcaagtcgcc gcgtgcggcc gtccagccgg cgttcgcgcg
     1681 tgcgtacaag acgatcgtgg cggccgacat cgtcaccatc ctcgcggccg gggtcctcta
     1741 cctgacggcc gtgagctcgg tccgcggctt cgcgctcacg ctgggagtgg cgacgctgct
     1801 cgatctgttc gtcgtgtggt tcttcaaacg cccgacggtg ttcctgatcg cgaagaacga
     1861 aaagctcgtg aacatgcgcg ggttcgggct cacgagcggt gtcgccgcgg aggtcgctcg
     1921 tgaaggagcg gcgctgtgaa cctccacgag gcgatcgaga cctatcgcgg ccacacgatc
     1981 ccccatgtcc gttgggtcga gcgccggaac atctggttcg cgatctccgg cgcgatggtc
     2041 ctcatctcgt tggtgggact gttgttcggc accctgaact tctcgatcga cttcgagggt
     2101 ggggctcgga tcagctacga gttcgccgaa ccggtcagcg tcgaggacgt ccaaaccacg
     2161 ctcgaggagc atgggataga cgacgccgag gttcagaccg tgaacgacga ggcgatctcg
     2221 atccgcacgc cttcgctctc ggcagaggga gagggcagcg cggaggcgat ccgccgcgat
     2281 ctggccgagc agtcggggat cgatccggtc gacgtgaaca tcgaggacgt ggggccgacc
     2341 tggggcggcg agatctcgcg caaggcagcg atcgggctgg tgatc
//
LOCUS       DASUVE010000084         2964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_791973, whole genome shotgun sequence.
ACCESSION   DASUVE010000084 DASUVE010000000
VERSION     DASUVE010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791973"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VF036_01755"
     CDS             <1..416
                     /locus_tag="VF036_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263986.1"
                     /translation="GVLVDTLEQALLSAEQERVVDREAMRLPEAPSAPSGKPRTSLHR
                     PDRQPTPESDPAVVRDEAEDDVAAAETPLADEVASTPPPEAAESPAAEPDPQTDDVPE
                     AQEGKEEESEVDRILLAKEFAGLLQVPREDDEASG"
     gene            468..2174
                     /locus_tag="VF036_01760"
     CDS             468..2174
                     /locus_tag="VF036_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX6263987.1"
                     /translation="MTTVKRKSKQLGQILIELGLITPEQLDAALAEHQKTPKALGRVL
                     IDLGMIKEADLVRALAEQVGLEFVDLNDTQIDPASTALLPEALARRYRALPIGERDGK
                     LLVAMSDPANVYALDDIRTITGREVQPVVATSADVEQAIQKFAGLDSQVEQLAAEAAE
                     GSEGEELDLEAALEDAPIVKLVNAIMTQAVAERASDVHIEPGERDVRIRFRVDGVLHE
                     PMRPQPKNLQGGLISRLKVMADLNIAEKRVPQDGRISMKVGTRQLDMRVATLPTVHGE
                     KVVIRVLDKSNAMLDLADLGFLEDSYDRFATAFSKPYGAILVTGPTGSGKSTTMYSTL
                     NILNEESKNIITVEDPVEYRMAGVNQIQVNPKAGLTFASALRSILRADPDIVLIGEIR
                     DKETATIAIEAALTGHLVLSSLHTNDAPSAISRLVEMDVETFLVASSIDAVVAQRLAR
                     ILCDKCKQPYAPDEAELRAAGYAEDSWADIGEVFRAPDNRVAKHECKACSGTGYRGRI
                     GLYEVMLRSEEIERLTVERASADAIRTVAIQQGMTTLREDGLAKVRAGITSIEEVARV
                     VK"
     gene            2176..>2964
                     /locus_tag="VF036_01765"
     CDS             2176..>2964
                     /locus_tag="VF036_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX6263988.1"
                     /translation="MSSVVQQDEDVQIPVPELLGALLEKGGSDLHLTAGSPPMVRVHG
                     ELEPAGEYPSLTPRALQGMVYAILPQKLRERFEQELELDMSYSLPGRSRFRVNVFQQR
                     DAVGAVFRVIPFEIKNMDDLGIPNVVMDLARFQRGFVVVTGPTGSGKSTTLAAMVDIV
                     NRERAGHIMTVEDPIEFLHRHKSCVVNQREVGADTHGFAQALKHVLRQDPDVILVGEM
                     RDLETIGTAITAAETGHLVFATLHTQDAPQTIDRIIDVFPPHQQQ"
BASE COUNT          540 a         1000 c         1007 g          417 t
ORIGIN      
        1 cgggcgtgct cgtcgacacg ctcgagcaag ccctcctctc cgccgagcag gagcgggtgg
       61 tggaccgaga agcgatgcgg ctgcccgaag cgccctccgc tccttcgggc aagccgcgca
      121 cgagcctgca caggccggac cgtcagccga ctcccgagtc agacccggcg gtcgtccgcg
      181 acgaggccga ggacgacgtc gccgcggccg agacgccact cgcggacgag gtcgcctcga
      241 cgcccccgcc ggaggccgcg gagtccccgg cggcggagcc cgaccctcag accgacgacg
      301 tcccagaggc ccaggagggc aaggaagaag agtcggaggt ggatcggatc ctcctcgcga
      361 aagagttcgc cggcctgctt caggtcccgc gggaggatga cgaagcaagc gggtagctgc
      421 atctgcacca cccatccggc ggaagaccta gagcggacgg accggcgatg accaccgtga
      481 agcggaagtc gaagcaactc gggcagatcc tgatcgagct cggattgatc acgcccgaac
      541 agctcgacgc ggcccttgcg gagcaccaga agaccccgaa ggcgctcggc cgggtcctga
      601 tcgacctcgg catgatcaag gaggccgacc tcgtccgtgc gctcgccgag caggtcggat
      661 tggagttcgt cgatctcaac gacacccaga tcgaccccgc ctccaccgcg ctcttgccgg
      721 aagcgctcgc tcgccgctac cgagccctcc cgatcggcga gcgcgacggg aagctcctcg
      781 tcgcgatgtc ggaccccgcc aacgtctacg ccctcgatga catccgcacg atcacgggcc
      841 gcgaggttca gccggtggtg gcgacgtccg ccgacgtcga gcaggcgatc cagaagttcg
      901 ccggcctgga ctcccaggtc gagcagctgg ccgccgaggc cgcggagggc agcgaggggg
      961 aggagctcga cctcgaggcc gccctcgagg acgcgcccat cgtcaagctc gtcaacgcga
     1021 tcatgaccca ggcggtcgca gagcgggcgt cggacgtcca catcgagccg ggagagcgcg
     1081 acgtgcgcat ccgcttccgc gtcgacggcg tgctccacga gccgatgcgg ccgcagccca
     1141 agaacctcca gggcgggttg atctcccggc tcaaggtcat ggccgatctg aacatcgcgg
     1201 agaagcgcgt accgcaggac ggccggatct cgatgaaggt cggcacccgg cagctcgaca
     1261 tgcgtgtcgc gacgctcccg accgtgcacg gcgagaaggt cgtgatccgc gtcctcgaca
     1321 agagcaacgc gatgttggac ctcgccgacc tcggcttcct cgaggactcc tacgatcggt
     1381 tcgccacggc gttctcgaag ccgtacggcg cgatcctcgt gacggggccg acgggttcgg
     1441 gcaagtcgac gacgatgtac tcgaccctca acatcttgaa cgaggagtcg aagaacatca
     1501 tcacggtgga ggatccggtc gagtaccgga tggccggcgt caatcagatc caggtcaatc
     1561 ccaaggcggg gctcacgttc gcctcggcgc tgcgatcgat cctgcgcgcc gaccccgaca
     1621 tcgtcctgat cggggagatc cgggacaagg agacggccac gatcgcgatc gaggccgccc
     1681 tgaccggtca cctcgtgctc tcctccttgc acacgaacga cgccccctcg gcgatcagcc
     1741 gtctcgtgga gatggacgtg gagacgttcc tggtcgcatc gtcgatcgac gccgtcgtcg
     1801 cccagcgtct cgcgcggatc ctctgcgaca agtgcaagca gccgtacgcc ccggacgagg
     1861 ccgagctccg ggcggcgggc tacgcggagg actcgtgggc ggacatcggc gaggtcttcc
     1921 gggcacccga taaccgggtc gcgaagcacg aatgcaaggc ctgctccggc acgggctacc
     1981 gggggcggat cgggctctac gaggtgatgc tccgaagcga agagatcgag cgactcacgg
     2041 tggagcgtgc atctgccgat gcaatcagga ccgtcgccat ccaacagggg atgacaacgc
     2101 tgcgggagga cggtctggcc aaggtccgag cagggatcac atcgatcgag gaagtcgcga
     2161 gggtggtgaa gtgagatgag ctcagtggtc caacaggatg aggacgtaca gatcccggtg
     2221 ccggagttgc tcggcgcgct cctggagaag gggggctccg acctgcacct cacggcgggt
     2281 tccccgccga tggttcgggt ccacggagag ctggagccgg cgggggagta cccgtcgctg
     2341 acaccgaggg ccctgcaggg catggtgtac gcgatcttgc cgcagaagct ccgggaacgg
     2401 ttcgagcagg agctcgagct cgacatgtcg tactcgctgc cgggacggtc ccggttccgt
     2461 gtcaacgtgt tccagcagcg cgacgccgtc ggcgccgtct tccgcgtgat cccgttcgag
     2521 atcaagaaca tggacgacct cgggatcccg aacgtcgtga tggacctcgc tcgcttccag
     2581 cggggcttcg tcgtcgtcac gggccccacg ggctcgggga agtcgacgac gctcgccgcg
     2641 atggtcgaca tcgtgaaccg tgagcgggcc ggacacatca tgaccgtcga ggacccgatc
     2701 gagttcctgc atcgtcacaa gagctgcgtc gtcaaccagc gtgaggtggg cgccgacacc
     2761 cacgggttcg cgcaggcgct caagcacgtg ctgcggcagg accccgacgt gatcctcgtc
     2821 ggcgagatgc gcgacctcga gacgatcgga accgcgatca ccgcggccga gacgggtcac
     2881 ctcgtcttcg ccacgctgca cacgcaggat gcgccccaga cgatcgaccg catcatcgac
     2941 gtgttcccgc cccaccagca gcag
//
LOCUS       DASUVE010000085         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_228517, whole genome shotgun sequence.
ACCESSION   DASUVE010000085 DASUVE010000000
VERSION     DASUVE010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228517"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /gene="leuD"
                     /locus_tag="VF036_01770"
     CDS             complement(<1..418)
                     /gene="leuD"
                     /locus_tag="VF036_01770"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX6263989.1"
                     /translation="MEPITRIEGTAVPLDRSDVDTDQIVPAQFLKRIERTGFGPFLFH
                     EWRKDPSFVLNDERYAGASILIAGANFGGGSSREHAPWALEDFGFRAVIAPSFADIFR
                     NNCCKIGLLPVELPQESVRALMDAVLDDPTTRIVVDL"
     gene            complement(419..1846)
                     /gene="leuC"
                     /locus_tag="VF036_01775"
     CDS             complement(419..1846)
                     /gene="leuC"
                     /locus_tag="VF036_01775"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009743223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX6263990.1"
                     /translation="MAQTVAEPKTLAEPKTLAEKVWERHVVHRAAGEPDLLYVDLHLV
                     HEVTSPQAFEALRLAGRPVRRPDLTVATMDHNVPTTDGPVTDRTSARQMEALATNAKE
                     SDITLYPWGSAGQGIMHVIGPEMGYTQPGLTIVCGDSHTSTHGAFGALAFGIGTSEVE
                     HVLATQTIPQTPARTMAITVDGDLPAGCGAKDVILGIINRIGTGGGVGHVIEYRGSAI
                     RALSMEGRMTICNMSIEAGARAGMVAPDDTTFAYLEGRPHAPSGADWERALDDWRSLP
                     TDAGATFDTEVTIDATALRPHVSWGTNPAQSVTIDDAVPEPDSFDDEAKRDAARRALA
                     YMALEPGTRIRDIRPDTVFIGSCTNSRIEDLRLAAGVVEGRSVAQGLRALVVPGSVAV
                     KLQAEREGLDRIFVDAGFDWRGAGCSMCLGMNPDILQPGERSASTSNRNFEGRQGKGG
                     RTHLVSPAVAAATAIAGHFTTPEDL"
     gene            1935..>2612
                     /locus_tag="VF036_01780"
     CDS             1935..>2612
                     /locus_tag="VF036_01780"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX6263991.1"
                     /translation="MIAGGDDQQERDRARFQRDVRDGELVRAGRPGQAEDLRRHLPTS
                     GVGVLDRCVAILRAVEDGARSFTDIVDVTGLTRSTTHRLIQGLEDHGFLLQIGGLGYA
                     LGPRLLALAAGASRDLPLRELAHPSLERLARSTGESAQLYVRDGDRRVCIDASESESE
                     LRTIVEIGASLPLTKGSAGTVFLAWAGAPDRARLVATADDPDRLERRLVTTRRRGWAE
                     SVAERAPG"
BASE COUNT          363 a          949 c          896 g          404 t
ORIGIN      
        1 ccaggtcaac gacgatccgg gtggtcgggt cgtccaggac tgcgtccatc agcgcccgca
       61 ccgactcctg tggcagctcg accgggagca atccgatctt gcagcagttg ttccggaaga
      121 tgtcggcgaa gctcggcgcg atcacggcgc ggaacccgaa gtcctccagc gcccacggcg
      181 cgtgctcgcg gctcgagccg cccccgaagt tggcccccgc gatcaggatc gaggcgccgg
      241 cgtagcgctc gtcgttcagg acgaacgacg gatccttccg ccactcgtgg aagaggaacg
      301 gaccgaagcc cgtgcgctcg atccgcttca ggaactgcgc aggcacgatc tggtcggtgt
      361 ccacgtcgga gcgatcgagc gggacggccg tgccctcgat gcgggtgatc ggctccatct
      421 agaggtcctc cggtgtcgtg aagtggcccg cgatcgcggt ggcggccgcg acggccggcg
      481 agaccaggtg cgtccgccca cccttgccct gtctgccctc gaagttccgg ttcgacgtgg
      541 aggcgctccg ctcgcccggc tgcaggatgt cggggttcat ccccaggcac atcgagcacc
      601 ccgcgccgcg ccagtcgaac ccggcgtcga cgaagatccg gtcgaggccc tcgcgctccg
      661 cctgcagctt cacggcgacc gagccgggca cgaccagcgc acggagtccc tgggccacgc
      721 tgcgtccctc gacgaccccg gcggccaggc ggaggtcctc gatccgtgag ttcgtgcagg
      781 agccgatgaa gacggtgtcc ggccggatgt cccggatcct cgttccgggc tcgagcgcca
      841 tgtacgccag ggcgcgccgg gcggcgtcac gcttcgcctc gtcgtcgaag gagtcgggct
      901 cgggaacggc gtcgtcgatc gtgaccgact gtgccgggtt cgtcccccac gacacgtgcg
      961 ggcggagcgc ggtggcgtcg atcgtgacct ccgtgtcgaa cgtcgcgccg gcgtcggtgg
     1021 ggagcgagcg ccagtcgtcg agggcccgct cccagtcggc gcccgacggc gcgtgcggcc
     1081 tcccctccag gtaggcgaac gtggtgtcgt cgggagcgac catccccgcg cgggcgccgg
     1141 cctcgatcga catgttgcag atcgtcatgc gaccttccat cgacagcgcg cggatcgcgg
     1201 acccgcggta ctcgatcacg tggccgactc cgcctccggt gccgatccgg ttgatgatgc
     1261 cgaggatcac gtccttggcg ccgcaccccg ccggcaggtc gccgtcgacg gtgatcgcca
     1321 tcgtcctggc gggcgtctgg gggatcgtct gtgtggcgag cacgtgctcg acctcgctcg
     1381 tgccgatccc gaacgcgagc gcgccgaacg cgccgtgcgt cgacgtgtgc gaatcgccgc
     1441 agacgatcgt gagacccggc tgcgtgtagc ccatctccgg tccgatcacg tgcatgatcc
     1501 cctgccccgc cgatccccac gggtagagcg tgatgtcgga ctccttcgcg ttcgtcgcga
     1561 gggcctccat ctgccgggcg ctcgtccgat cggtcaccgg tccgtcggtc gtggggacgt
     1621 tgtggtccat cgtcgcgacg gtgagatccg gccggcggac ggggcgtccc gcgagccgga
     1681 gggcctcgaa cgcctgcggg ctcgtcactt catggacgag atgcagatcg acgtagagca
     1741 gatccggctc accggccgcc cgatgcacga cgtggcgctc ccagaccttc tcggcaaggg
     1801 tcttgggctc ggccagggtc ttcggctccg ccacggtctg cgccaccgtt ccgtcagcct
     1861 cctgcgacca ggttccccgt tggtcgccca cgctgttcca catagtggaa ctcagtcccg
     1921 tatagtggaa caggatgata gcaggagggg acgaccagca ggaacgagac cgtgcccggt
     1981 tccaacgcga cgtccgggac ggcgagctcg tgcgggccgg gcgtccaggc caagccgagg
     2041 acctccgccg acacctcccg accagcggcg tcggtgtgct cgaccgctgc gtcgcgatcc
     2101 tccgcgcggt cgaggacggt gcccggtcgt tcaccgacat cgtcgacgtg accggcctca
     2161 cccgttccac cacgcaccga ctgatccaag gtctcgagga ccacggcttc ctgctccaga
     2221 tcggcggtct cggctacgcc ctcggccccc gcctgctcgc cctcgccgcc ggcgcgtcgc
     2281 gggatctccc cttgcgagag ctcgcgcacc cctcgctcga acgcctcgcg cgctccaccg
     2341 gtgagagcgc ccagctctac gtccgagacg gcgaccgaag ggtctgtatc gacgcctccg
     2401 aatccgagag cgagctccgc acgatcgtcg agatcggcgc ctcgctcccg ctcacgaagg
     2461 gttcggcagg gacggtcttc ctcgcctggg ccggcgcgcc cgatcgcgcc cgcctcgtcg
     2521 cgaccgccga cgatcccgat cggctggaac ggaggctcgt gacgacccgc cggcgcggat
     2581 gggcggagag cgtcgccgag cgggcgcccg gc
//
LOCUS       DASUVE010000086         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1547535, whole genome shotgun sequence.
ACCESSION   DASUVE010000086 DASUVE010000000
VERSION     DASUVE010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547535"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VF036_01785"
     CDS             <1..351
                     /locus_tag="VF036_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX6263992.1"
                     /translation="AAIDAVHPLLPEGHANEPATTFRFADGAPGRVGVIASVTEPFCD
                     TCNRLRLTAEGMFRNCLFAMDETDLRAPMRDGASDDDLALLIRQGLWTKWSGHRINHP
                     DFVQPARSMSMIGG"
     gene            complement(525..1385)
                     /locus_tag="VF036_01790"
     CDS             complement(525..1385)
                     /locus_tag="VF036_01790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEX6263993.1"
                     /translation="MKARTVLDDAVKVLKASPAIDHWQRGRERIEAEDLLWHVLGEEP
                     VPRDDVTVEQRRTFDRLVARRATGEPIPFIKGSTEFRGLDLLVRPGVFVPRDASEFLA
                     EQAMRRLRGRARPVHLDLATGAGTIALAVANEVRGADVVGTDLSADAVKLARTNAKRL
                     GVRARFFTGDLFGGLPRTLKGTVDVATIHPPYVPMHELSDLPEEIRDWEPEHTLTDRS
                     PDGLGLISRSAREAPSWLSPNGWLLLEVSPDRVHHVKGVLRGEGYRDIRSTKGGPLPL
                     TRVIVARRPR"
     gene            1428..1838
                     /locus_tag="VF036_01795"
     CDS             1428..1838
                     /locus_tag="VF036_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX6263994.1"
                     /translation="MPGATPADGAKSTRTAKPRRARAKKPTSTGEDLRLTGDRLVVQL
                     PENGERKSASGLLIPATAAPAPKRLAWADVKLVGPDVRVAAQGDRVLFLPSSGLEVEL
                     DGQELVLLRERDVQAVSSPPASDARSQRAPGQYL"
     gene            complement(1889..2293)
                     /locus_tag="VF036_01800"
     CDS             complement(1889..2293)
                     /locus_tag="VF036_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX6263995.1"
                     /translation="MRTTEFELSTKGRRVVDLTDRVGDFAGASGGDGLLHVFLPHATA
                     GLALMETGSGSEEDLDAALERLLPRDDRYAHRHGSVGHGGDHLLPVFVAPTLTLLVEE
                     GRLVLGTWQSIVLIDPNREHDVRRVRLTFIEG"
BASE COUNT          346 a          869 c          811 g          348 t
ORIGIN      
        1 gctgcgatcg acgccgtgca tcccctcctc cccgagggac acgcgaacga gccggccacg
       61 acgttccgct tcgccgacgg agcccccgga cgggtcggcg tgatcgcctc cgtcaccgag
      121 ccgttctgcg acacgtgcaa ccgactgcgg ctgacggccg agggcatgtt ccgcaactgt
      181 ctgttcgcga tggacgagac ggatctgcga gcgccgatgc gcgacggtgc ttccgacgac
      241 gacctggcgc tcctgatcag gcagggtctc tggacgaagt ggtccggtca ccgcatcaac
      301 cacccggact tcgtgcaacc cgcgcgatcg atgtcgatga tcggcggctg atcgcagcag
      361 gttccaccgc cggctcatcg tctcgacacg aggcgttcac ctccgactgc gagcctggcg
      421 tcgccgtgga ggctgcgccc cctctccggc gcggggagcc ggccgcaggt cggcttcccg
      481 cgtaccccct ccggcgagcg ccacgccgaa ccgtgccgcg accgtcaccg gggacgcctc
      541 gcgacgatga cccgcgtcaa cggcagaggg ccccctttcg tgctgcggat gtcgcggtag
      601 ccctcgccgc gcagcacgcc cttcacgtgg tgcacccgat ctgggctgac ctcgagcagg
      661 agccacccgt tgggggagag ccatgagggt gcctcccggg ccgaccggga gatcagcccg
      721 agcccgtcgg ggctccgatc ggtcaacgtg tgctccggct cccagtcccg gatctcctcg
      781 ggcaggtcgc tgagttcgtg catcggcacg tacggcggat ggatcgtggc gacgtccacg
      841 gtgcccttga gcgtccgcgg gaggccgccg aacaggtcgc cggtgaagaa gcgggctcgc
      901 acgccgagcc gcttcgcgtt cgtgcgagcg agcttgacgg catccgcgga gaggtcggtc
      961 ccgaccacat ccgcgccgcg gacctcgttc gccaccgcca gcgcgatcgt ccccgctccg
     1021 gtcgccaggt cgagatggac cggccgtgct cggccgcgca ggcgccgcat cgcctgttcg
     1081 gcgaggaact ccgacgcatc ccggggaacg aagacccccg gacggacgag cagatccagg
     1141 cctcggaact ccgtggagcc cttgatgaag gggatgggct caccggtggc gcgacgagcg
     1201 accagccgat cgaacgttcg tcgctgctcg acggtgacgt cgtcgcgcgg aaccggctcc
     1261 tcgcccagca cgtgccagag caggtcctcc gcctcgatcc gctcccgtcc acgttgccaa
     1321 tggtcgatcg cgggcgaggc cttcaggacc ttgaccgcgt cgtccaggac cgtgcgtgcc
     1381 ttcaacgccg ggcatcctac cgagtgacgc tcgctagact cggatgcatg cccggagcga
     1441 cgcccgccga tggcgcgaag tcgacgagga cggcgaagcc tcgtcgcgcg cgcgcgaaga
     1501 agccgacgtc gacaggggag gatctgcgtc tgaccggaga ccggctcgtc gtgcagttgc
     1561 ccgagaacgg cgagcggaag tcggcgagcg gcctgctgat ccccgccacg gcagcgccgg
     1621 cgccgaagcg tctggcgtgg gccgacgtca agctcgtggg ccccgatgtg cgcgtcgcgg
     1681 cccagggcga ccgcgtcctg ttcctcccgt cctcgggcct cgaggtggag ctggacggac
     1741 aggagctcgt cctcctccgt gagcgggacg ttcaggcggt ctcgtcgcca ccggcgtcgg
     1801 acgcgcgctc ccagcgagct ccgggccagt acctgtaacg ccggcccctc ttcatcgcgg
     1861 cggccgctct tctcgtggcg gcccctccct agccctcgat gaacgtcagc cgcacgcgtc
     1921 ggacgtcgtg ctcgcggttc ggatcgatca gcacgatcga ctgccacgtc ccgagcacga
     1981 gccgcccctc ctccaccagg agggtgagcg tcggggccac gaacaccggc aggagatgat
     2041 cacccccgtg ccccacggag ccgtgccggt gcgcgtaccg gtcgtcgcgg ggaagcaacc
     2101 gctccagcgc cgcgtccaga tcctcctcgg agccgctgcc cgtctccatc agggcgaggc
     2161 ccgccgtggc gtgcgggagg aacacatgga gcaggccgtc gccgcccgac gctccggcga
     2221 agtccccgac ccggtccgtg aggtcgacca cgcggcggcc cttcgtcgaa agctcgaact
     2281 cggtcgtccg cacgctctcg aaccatactc gccgtcacgg tggggtggcc gggccctggt
     2341 gcgccgaggg tgtaatgatg taatctcgct tgca
//
LOCUS       DASUVE010000087         3345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_41714, whole genome shotgun sequence.
ACCESSION   DASUVE010000087 DASUVE010000000
VERSION     DASUVE010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41714"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VF036_01805"
     CDS             complement(<1..197)
                     /locus_tag="VF036_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263996.1"
                     /translation="MSRAGTARRATVIAIVPLLVAASDPTGDVAGCGGSPGAGTAPDL
                     VDARGEIVELGTSARWTLTFA"
     gene            complement(197..1630)
                     /locus_tag="VF036_01810"
     CDS             complement(197..1630)
                     /locus_tag="VF036_01810"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6263997.1"
                     /translation="MRRARVRQRRQTFGALVVVLTSILAWQFWPSAGQERGGPPEPGG
                     DGNGGQSPSPDPEQSSPIEHVIFLVKENRSFDHYFGKYPGAEGATEGGTIEDCDATTF
                     QDGPVIDLEEAPLVLPHDLGHAFAPGLYSINGGKMNGFNCVSLGDDLMGYSQHSRETL
                     PAYWAYADRFVLADHFFTSMYGPTFPEHLYTVAAQSYGIVDNKTTTNVEGNYCDDDTE
                     ITKRFPIEDLTDSDVEDIMALEEDITSDVPGQLIRIAAYWESTRTCINIPVLPDKLER
                     KGISWKYYANADSWMNALQAVEHVRSGPMWDKVQPPDTFLQDVRRDELPAVSWLIPPE
                     GHPNEHPGSMTNVCEGENWTVEYLNAVMRSDAWPHTAIVIVWDDFGGFYDHVVPPHYD
                     IMGLGPRTPALIISPWTKAGDNPDGGSIDSTVYEFSSVLRFIEDLHGLKPMTDRDGQA
                     DPLSGAFDFEQEPRLEPLIIEPRDCPS"
     gene            complement(1667..3049)
                     /locus_tag="VF036_01815"
     CDS             complement(1667..3049)
                     /locus_tag="VF036_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX6263998.1"
                     /translation="MSLAPPGRRMAGAALVALAGLGAAILAPQPSERPDDRPNVIVVL
                     TDDQRAGSFAAMPWLASQLARAGTGWAEVPNAFANTPLCCPARASLLTGLFARHTGVL
                     DNGDGARCDAASTLATWLHEEGYRTGLVGKYLNRYPFGRLPFVPAGWDRFLAKRNQTG
                     ATVYRDFRAVDQGSPVSVRRYATDWLADRAIGFVRSAPASQPFFLLFAPSAPHAPWIP
                     AARHQGTLAGLAIEEPPSVVGTLRGAPPWVRSLPAPSGAQRAAWLEEQRRADETLLAV
                     DEALHALVSALGDRLDDTVIFVLSDNGYSFGEHRWEGKTCPYEACVRVPLAVHASGLS
                     RGQREELVSMVDLAPTILDLAGVGAPATMDGTSFARTIGAGEVRSDPTDAVFLEWAGD
                     ERIPAWVAVRTSDFKLIRYADGFEELYDLSGRRWSADPWETTNRATDPRAAGLLRGLR
                     ALLGRVLGPG"
     gene            complement(3046..>3345)
                     /locus_tag="VF036_01820"
     CDS             complement(3046..>3345)
                     /locus_tag="VF036_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6263999.1"
                     /translation="PWIWVAWGDQDEELPTTREMRADYFDMRSVESTDPSWRTTEFQL
                     TYGDWIRLFRANGLLIENLIELRPDADATTTYDDYASLEWARAFPAEHIWKVRKG"
BASE COUNT          507 a         1164 c         1156 g          518 t
ORIGIN      
        1 tccgcgaacg tgagcgtcca gcgagcggag gtgccgagct cgacgatctc cccgcgcgcg
       61 tccacgaggt cgggcgcggt gccggcgccg ggcgagccac cgcacccggc aacgtcgccg
      121 gtgggatcgg atgcggccac gaggagcggg acgatcgcga tcacggtcgc gcggcgcgcc
      181 gtgccggcgc gcgacatcag gacgggcagt cccgcggttc gatgatcagc ggttcgagac
      241 gcggctcctg ctcgaagtcg aacgctccgg agagcgggtc cgcctgcccg tccctgtcgg
      301 tcatcggctt cagaccgtgc agatcctcga tgaaccgtag cacggaggag aactcgtaca
      361 cggtggagtc gatcgacccg ccgtccgggt tgtcgcccgc cttcgtccac ggcgagatga
      421 tcagcgcagg tgtccgtggg ccgagcccca tgatgtcgta gtgcggcggg acgacgtggt
      481 cgtagaagcc tccgaagtcg tcccagacga tcacgatcgc ggtgtgcggc caggcgtcgc
      541 ttcgcatcac cgcgttgagg tactcgaccg tccagttctc gccttcgcac acgttcgtca
      601 tcgatcccgg gtgctcgttc gggtgaccct cgggtgggat caaccaggag accgccggga
      661 gctcatccct ccgcacgtcc tggaggaacg tgtcgggcgg ctggaccttg tcccacatcg
      721 ggccggatcg cacgtgctcg accgcctgca acgcgttcat ccacgagtcg gcgttcgcgt
      781 agtacttcca gctgatcccc ttcctctcga gcttgtcggg gagcaccggg atgttgatac
      841 acgtccgtgt ggattcccag tacgcggcga tccggatcag ttgcccggga acgtccgacg
      901 tgatgtcttc ctcgagggcc atgatgtcct ccacgtccga gtccgtgagg tcctcgatcg
      961 ggaaccgctt cgtgatctcc gtgtcgtcgt cgcagtagtt gccctccacg ttcgtggtcg
     1021 tcttgttgtc gacgatgccg taggactgcg cggccaccgt gtagaggtgc tcggggaacg
     1081 tcggcccgta catcgacgtg aagaagtgat ccgcgaggac gaaccggtcc gcgtacgccc
     1141 aataggcagg cagggtctcc cgcgagtgct gcgagtagcc catcaggtcg tcaccgagcg
     1201 acacgcagtt gaagccgttc atcttgccgc cgttgatcga gtacaagccg ggagcgaacg
     1261 cgtggccgag gtcgtgcggg aggacgagcg gcgcctcctc gaggtcgatg acggggccat
     1321 cctggaatgt cgtcgcgtca cagtcctcga tcgtgcctcc ttccgtcgct ccctcggcgc
     1381 ccggatactt cccgaagtag tgatcgaagg accggttctc cttgacgagg aagatcacgt
     1441 gctcgatcgg tgagctctgt tcgggatcgg gcgaagggga ctggccgccg ttcccgtcgc
     1501 cacccggctc ggggggtccg cctcgctcct gaccggcgga gggccagaac tgccacgcga
     1561 ggatcgacgt caggacaacg acgagtgccc cgaatgtctg tcggcgctgg cgaacacgtg
     1621 cgcggcgcac aggtcggatg atcggcccgg gtcgcatcgg gccagtctag ccggggccca
     1681 ggacgcgacc gaggagggca cgcaggcctc ggaggagccc tgcggcgcgg ggatccgtcg
     1741 cccggttcgt cgtctcccaa gggtcggcgg accatcgcct cccgctcagg tcgtagagct
     1801 cctcgaagcc gtccgcgtac cggatcagct tgaagtcgga ggtgcggacg gcgacccacg
     1861 cggggatccg ctcgtcgccc gcccactcca ggaacaccgc gtccgtcggg tcgcttctga
     1921 cctctcccgc cccgatcgtc cgcgcgaagc tcgttccgtc catcgtcgcg ggcgccccga
     1981 cgccggccag atccaggatc gtcggagcca ggtccaccat cgagacgagc tcctcccgct
     2041 gtccccggga caggccggag gcgtgcaccg cgagcggaac ccggacacac gcttcgtacg
     2101 gacacgtctt gccctcccac cgatgctccc cgaaggagta gccgttgtcc gacagcacga
     2161 agatcaccgt gtcgtccagg cggtcgccga gcgcggaaac gagcgcgtgg agcgcctcgt
     2221 cgacggcgag cagggtctcg tccgcacgcc gctgctcctc gagccaggcg gcgcgctgcg
     2281 cgccgctcgg cgccggcaac gaacgcaccc acggcggggc gccgcgcagc gtccccacca
     2341 cggagggagg ctcctcgatc gccaggccgg cgagcgtgcc ctgatgccgg gccgcgggga
     2401 tccacggagc atgcggggcg ctcggcgcga acaggaggaa gaacggctgc gacgcgggcg
     2461 cgctccggac gaagccgatc gctcggtccg cgagccagtc ggtcgcgtag cgacggaccg
     2521 agaccggcga cccctggtcg acggcccgga agtcgcgata cacggtcgcc cccgtctggt
     2581 tccgcttcgc caggaaccgg tcccaacccg cgggcacgaa cgggagtcgc ccgaacgggt
     2641 accgattcag gtacttcccc acgaggcccg tgcggtagcc ctcttcatgg agccacgtgg
     2701 cgagcgtgga cgccgcgtcg cacctcgcac cgtccccgtt gtcgaggaca ccggtgtgcc
     2761 gagcgaacag gcccgtcagc aggctcgccc gcgccgggca gcacagcggc gtgttcgcga
     2821 acgcgttcgg gacctccgcc cagcccgtgc cggcgcgcgc gagctgcgac gcgagccacg
     2881 gcatcgccgc gaaggagccc gctcgctggt cgtcggtcag gacgacgatc acgttgggtc
     2941 ggtcgtcggg gcgttcgctt ggctgcggag cgaggatcgc cgcgccgagc ccggccagcg
     3001 cgacgagggc cgccccggcc atccgacggc cgggcggcgc gagactcacc ccttccgcac
     3061 cttccagatg tgttccgcgg ggaacgctcg ggcccactcc agggatgcgt agtcgtcgta
     3121 cgtcgtggtc gcgtccgcgt ccggccggag ctcgatcagg ttctcgatca ggagcccgtt
     3181 cgcgcggaac aaccggatcc aatcaccgta ggtgagctgg aactcggtcg ttcgccacga
     3241 cgggtcggtg gactcgaccg accgcatgtc gaagtagtcc gcgcgcatct cccgggtcgt
     3301 ggggagctcc tcgtcctggt cgccccacgc gacccagatc cacgg
//
LOCUS       DASUVE010000088         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1362129, whole genome shotgun sequence.
ACCESSION   DASUVE010000088 DASUVE010000000
VERSION     DASUVE010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362129"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..245)
                     /locus_tag="VF036_01825"
     tRNA            complement(175..245)
                     /locus_tag="VF036_01825"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Gly,seq:tcc)
     gene            287..1159
                     /locus_tag="VF036_01830"
     CDS             287..1159
                     /locus_tag="VF036_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX6264000.1"
                     /translation="MEIGFRLVDAFTETPFEGNRLCVVPEIPEGLSDDQMRMLALETN
                     FSETTFVTDSHAGGYRMRIFTPDAELPFAGHPTIGTAFTLASEGRTSERTVQTTALGD
                     VPVEVDLERGFAWMTQQRPVFGDEFDDRAVVAEAAGLQPDDLDPTLPMQVITTGLGPL
                     LVPIKDEATLRRAERYASGCRRATEASGGECLYLFAIRGDGDVLARMFDASFGVGEDP
                     ATGSAAGPLGVYLASRGLAGMPGEAVVAQGEMVGRPSFLHLDVRPDDGSWMVRVGGGV
                     RIVGEGVFRVAIGD"
     gene            complement(1156..1554)
                     /locus_tag="VF036_01835"
     CDS             complement(1156..1554)
                     /locus_tag="VF036_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264001.1"
                     /translation="MRSRSGARSTVTTVLSIALIISIAGFAVLVGLQVRADHPARFDV
                     TQAQGVECPVGEGSPACFSFGVTNLGSQPSLVECIATAPTGLASFLNDMPVYTSSAPF
                     EAGVTEQLIVKVDQGDQGAVSEPALVCRAL"
     gene            1593..1666
                     /locus_tag="VF036_01840"
     tRNA            1593..1666
                     /locus_tag="VF036_01840"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1627..1629,aa:Pro,seq:tgg)
     gene            complement(1674..2807)
                     /locus_tag="VF036_01845"
     CDS             complement(1674..2807)
                     /locus_tag="VF036_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX6264002.1"
                     /translation="MTRYRDLGLSVGLLSPGPMNAITDVAGVRVGVTTLIEGDGPLEV
                     GTGPIRTGVTAIVPHEGIGEAPLFAGCHTLNGNGELTGLEWVRESGLLTTPIAITNTH
                     SVGVVRDALVAYEVRNRPKGPAFWSLPVVGETYDGALNDINGQHVRPAHLFAAIEHAS
                     DGPVPEGCVGGGTGMICHDFKGGTGTASRIVEGGQGNGWTVGALVQANHGDRERLAVD
                     GVHIGEAIPTSEIPSAWDEEERIGSTAEGSIIVIVATDAPLVSHQLRRLAQRVSLGIA
                     RVGGGGGDSSGDIFLAFSTANAGRITTSKTDAGPAPISVEMLPNREITSLFWGVIEAT
                     EEAILNALVAAETLTGRDGITAHALPHDRLVETMTKHGRGPNR"
BASE COUNT          426 a          980 c         1009 g          440 t
ORIGIN      
        1 ctgcggcttg agctcagacc gcgagcaggg ggacgcttcg ccgggttggt ccccaccggt
       61 cccgcagcag tcccgcgaag acggcgaacc agcaggaagc aacgagcaac gtcgatcgac
      121 gcgaatccgg cgaacacctc agctgacctg cggagatatg cccctgacct ggagagcggg
      181 tgacgggaat cgaacccgca ccaccagctt ggaaggctgg agctctacca ttgagctaca
      241 cccgcgcgga gccagcctag caacacccgg ctgggatact tcgccgatgg agatcggttt
      301 ccggctcgtc gacgcgttca ccgagacacc cttcgaaggc aaccggctct gcgtcgtgcc
      361 cgagatcccg gagggcctct ccgacgatca gatgcggatg ctcgcgctcg agacgaactt
      421 ctccgagacg acgttcgtga ccgattcgca tgccggcggc tatcggatgc ggatcttcac
      481 gccggacgcc gagctgccct tcgccgggca tcccacgatc gggacggcgt tcaccctcgc
      541 cagcgagggg cggacgtccg agcgaacggt ccagacgacc gcgctcggcg acgtgccggt
      601 cgaggtcgac ctcgaacgcg gcttcgcctg gatgacccag caacgtccgg tgttcgggga
      661 cgagttcgac gatcgcgcgg tcgtcgctga ggccgccggt ctgcaacccg acgacctcga
      721 tcccacgctc ccgatgcagg tgatcacgac cgggctcggc ccgttgctcg tgccgatcaa
      781 ggacgaggcc accctgcgac gggcggaacg ttacgcgtcg ggctgccggc gcgcgaccga
      841 ggcttcgggt ggcgagtgcc tctacctgtt cgcgatccga ggggacggcg acgtgctcgc
      901 gcggatgttc gacgcgtcct tcggtgtcgg cgaggatccc gcgacaggct cggccgccgg
      961 cccgctcggc gtctatctgg catcgcgcgg cctggcgggg atgccggggg aggccgtggt
     1021 cgctcagggg gagatggtgg ggcggccgag cttcctccac ctcgacgtcc ggcccgacga
     1081 cggctcgtgg atggtgcggg tgggcggcgg cgtccggatc gtcggtgagg gcgtcttccg
     1141 cgtggcgatc ggggactaga gcgccctgca gaccaacgcc ggttcggaca cggcgccctg
     1201 gtccccctga tcgaccttga cgatcagctg ctcggtgacc ccggcctcga acggtgccga
     1261 gctggtgtac accggcatgt cgttcaggaa gctggcgagg ccggtcgggg ccgtcgcgat
     1321 gcattccacg agggacggct ggctgccgag gttcgtcacg ccgaagctga agcatgccgg
     1381 actgccttcg ccgaccgggc actccacgcc ctgggcctgg gtgacgtcga agcgagccgg
     1441 gtggtcagcg cgaacctgca agcccaccag gactgcgaac cctgcgatgg agatgatcaa
     1501 cgcgatcgag aggacggtgg tgacggtgct ccgggccccg gaccgcgaac gcatgcccca
     1561 gggtaccggg acgtagaatc gctgcacaag agcggggcgt agcgcagcct ggcagcgcac
     1621 gtgctttggg agcacgaggt cgcgggttcg aatcccgccg ccccgaccga cggctaacgg
     1681 ttcggtcccc gcccgtgctt cgtcatcgtc tccaccaatc ggtcgtgggg gagcgcgtga
     1741 gcggtgatgc cgtcgcggcc ggtcagcgtc tcggccgcca cgagcgcatt caggatcgcc
     1801 tcctcggtgg cttcgatcac gccccagaac agcgaggtga tctcccggtt cgggagcatc
     1861 tcgacgctga tcggggcggg gccggcatcg gtcttcgagg tcgtgatccg tccggcgttc
     1921 gcggtggaga acgcgaggaa gatgtccccg ctggagtcac ctccgccgcc gccgaccctc
     1981 gcgatcccga ggctcacccg ctgggccagc cgccggagct gatgcgagac gagcggggcg
     2041 tccgtggcga cgatcacgat gatcgatccc tcggcggtcg agccgatccg ctcctcctcg
     2101 tcccacgcgg acgggatctc actcgtgggg atcgcttcgc cgatgtgcac gccgtcgacc
     2161 gcgagccgtt cgcggtctcc gtgattggcc tgcacgaggg ccccgacggt ccatccgttc
     2221 ccttggcctc cctccacgat ccgggacgcg gtcccggtgc cgcccttgaa gtcgtggcag
     2281 atcatcccgg tgccgccgcc gacgcagccc tcggggaccg ggccgtccga ggcgtgctcg
     2341 atcgcggcga agaggtgtgc cggacggacg tgctgcccgt tgatgtcgtt cagcgcgccg
     2401 tcgtaggtct cgccgaccac ggggaggctc cagaacgccg gacccttcgg gcggttccgc
     2461 acctcgtagg cgacgagggc gtcgcggacg acgccgacgc tgtgcgtgtt cgtgatcgcg
     2521 atcggcgtgg tgaggagccc cgactctcgc acccattcga gccccgtcag ctcgccgttg
     2581 ccgttgagcg tgtggcagcc ggcgaagagg ggcgcctcgc cgatcccttc gtgcgggacg
     2641 atcgccgtca cgcccgtccg gatgggcccg gtaccgacct cgagcgggcc gtccccttcg
     2701 atcagggtgg tgacacccac gcggacaccc gcgacgtccg tgatcgcgtt catcggcccg
     2761 ggggacagca aacccacgga caagccgagg tcgcggtagc gcgtcatcgg ggtcgaaccc
     2821 tacagcccta gacgagcgcc ctcgtggcgg cggtg
//
LOCUS       DASUVE010000089         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1362275, whole genome shotgun sequence.
ACCESSION   DASUVE010000089 DASUVE010000000
VERSION     DASUVE010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362275"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..361
                     /locus_tag="VF036_01850"
     CDS             113..361
                     /locus_tag="VF036_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264003.1"
                     /translation="MRRFVIGGALGVFIISVVLAACGGSNADSTGDQARRFQDMWEID
                     KIEKDFHGAMSDKDIDLMMSLFAPNATMTVGPGRPRRG"
     gene            367..618
                     /locus_tag="VF036_01855"
     CDS             367..618
                     /locus_tag="VF036_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6264004.1"
                     /translation="MRTFWQENSATFDPENPWISDHPAYKLEITVDGDRGTLHFECHF
                     VDADTGEVVAATAADLDVARIDGQWLITNMIGGTTVLEV"
     gene            623..2263
                     /locus_tag="VF036_01860"
     CDS             623..2263
                     /locus_tag="VF036_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6264005.1"
                     /translation="MSEEQEPRVAGAERRRIDDPLVHAVARLPLKVRAKMLIAFVGTS
                     LLLVAVGLLGQIVLGQSNDRVGSVGPLQRRAVAYGQLQTETEHLRDVLAENVGPAYNV
                     TWPETAPSEERGVFSLPVDLLAADAADRVKSRTATDRLGFTPSREEQAFLGSMAATAD
                     ELERLLKQDIIPIYAGEATISIQDEETIKQMVPLRADAEALARDLYRDTTSLADGTRH
                     EIRELIARNFSSFESSRALFIGVAAGALVLALLLGFVLSWSLIGPIQQIDTRLASIAS
                     GDFSGHVEVENRDELGALATNVNRMNDELRRVYAELEEASRHKSEFLANMSHELRTPL
                     NAIIGFSQVLRDEMVGSVNPKQAEYLDDIISSGNHLLSLINDVLDLSKVEAGQVELDV
                     HPFSLRETLERGVVMVRERATEDGVRVSFSADPDVDVMDGDERRIKQVIFNLLSNAVK
                     FTPAGGEIDVNAIRENGEVRISVADTGPGIAPEDRERIFEEFQQTEAGVAQREGTGLG
                     LTLSKRLVELHGGRIWLESELGEGSTFSFTLPLARVSS"
BASE COUNT          418 a          704 c          804 g          386 t
ORIGIN      
        1 tggcctggtg ggcctgggcc gttgcgctcc ttagcctcta gacggatcgg atcggcggtg
       61 tgaacatcct cggcaccgac gaccaggcag cttgagcgga agggggcatc acatgcggag
      121 gttcgtgatc ggcggcgcac tcggggtctt catcatctcg gtcgtgctcg cagcgtgcgg
      181 tgggtcgaac gccgactcga ccggcgacca agcccgacga ttccaggata tgtgggagat
      241 cgacaagatc gagaaggact tccacggcgc catgtcggac aaggacatcg atctgatgat
      301 gagcctgttc gctccgaacg caacgatgac ggtgggtccg gggagaccgc gtcggggctg
      361 aacgagatcc ggacgttctg gcaagagaac tcggcgacct tcgatcccga gaacccgtgg
      421 atctcagacc accctgcgta caagctcgag atcacggtcg acggagatcg gggcacgctc
      481 cacttcgagt gccatttcgt ggatgcggat acgggcgagg tcgtggctgc cacggcggct
      541 gatctcgacg tggctcggat cgacgggcaa tggctgatca cgaacatgat cggcggaacg
      601 accgtcctgg aagtctgacg tcttgtcgga ggaacaggag ccgcgcgtcg ccggcgcaga
      661 acggcgacgg atcgacgacc cgctcgtcca tgccgtcgcc cgactgcccc tcaaggttcg
      721 cgcgaagatg ctgatcgcgt tcgtcggtac ctccctgctg ctcgtcgcgg tcggcctgct
      781 gggacagatc gtgctcgggc agtcgaacga tcgagtcggg agcgtcgggc cactccagag
      841 acgcgccgtc gcgtacgggc agctccagac cgagaccgag catctccggg atgtactggc
      901 ggagaacgtc gggcccgcct acaacgtgac ctggcctgag acggcgccgt ccgaggagcg
      961 cggtgtcttc tcgttgccgg tggatctcct cgcggcggac gctgccgacc gcgtcaagtc
     1021 gaggaccgcc acggatcgac ttggattcac gccgtctcgg gaagagcagg ccttcctcgg
     1081 gagcatggca gccacggccg atgagctcga gaggttgctc aagcaggaca tcatcccgat
     1141 ctacgcgggc gaggcaacga tctcgatcca ggacgaggag acgatcaaac agatggttcc
     1201 gctgcgagcc gacgcggaag cgctcgctcg cgacctctat cgggacacta cgtcgctggc
     1261 ggacgggacc cgacacgaga tccgagaact catcgctcgg aacttcagct cgttcgagag
     1321 ctcccgggca ctgttcatcg gcgtcgcggc gggagcgctc gtcctcgccc tcctgctggg
     1381 gttcgtcttg tcgtggtcgc tgatcggccc gatccagcag atcgacacgc ggctggcctc
     1441 gatcgcgtcg ggtgacttct cgggacacgt ggaggtggag aaccgcgacg agctgggtgc
     1501 cttggcgacc aacgtcaacc ggatgaacga cgagctccgg cgcgtgtacg cggagctcga
     1561 ggaggccagc cggcataagt ccgagttcct ggccaacatg tcccacgagc tgcggacgcc
     1621 gctcaacgcg atcatcggct tctcgcaggt gctgcgagac gagatggtcg gttccgtgaa
     1681 cccgaaacaa gcggagtacc tcgacgacat catctcctcg gggaaccatc tgctctcgct
     1741 gatcaacgac gtcctcgatc tctccaaggt cgaggcgggg caggtggagc tggacgtgca
     1801 cccgttctcc ttgcgcgaga cgctcgagcg cggtgtggtg atggtgcggg agcgggcgac
     1861 ggaggacggc gtgcgcgtct cgttctccgc ggatccggac gtcgacgtca tggacggcga
     1921 cgagcgacgg atcaagcagg tgatcttcaa cttgctctcc aacgcggtga agttcacgcc
     1981 tgccggcgga gagatcgacg tgaacgcgat ccgcgagaac ggggaggtgc ggatctcggt
     2041 ggccgacacg ggacccggca tcgcccccga ggatcgggaa cggatcttcg aggagttcca
     2101 gcagaccgag gccggggtcg cgcagcggga ggggaccggg ctcggcttga cgctctcgaa
     2161 gcgactcgtc gagcttcacg gcggtcggat ctggctcgag agcgagcttg gggaggggag
     2221 caccttctcc ttcacgctcc cgctcgcgag ggtgtcgtca tgacgggcga gcgggtcctc
     2281 gtcgtcgagg acaacgagaa gaacatgaag ct
//
LOCUS       DASUVE010000090         3632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1173607, whole genome shotgun sequence.
ACCESSION   DASUVE010000090 DASUVE010000000
VERSION     DASUVE010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173607"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /gene="rho"
                     /locus_tag="VF036_01865"
     CDS             complement(<1..1326)
                     /gene="rho"
                     /locus_tag="VF036_01865"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX6264006.1"
                     /translation="METMDRSTLEGKLLPELQQIAQTMGIEGTQRLRKAGLIDAIVEA
                     SSNGERPGNGEARSAASVESAVATVERAPASVRDADEGDNGDDGDDGDESQPSDGVND
                     RSAASVETASPRRDDRRPGGEDRPRDDRQQGGRGGSRPQGGDRDDRGDSGDRNDPRNR
                     RRRPTREERRQMREERREREEQERIEALKTAPSASGIHDLLPDGYGFMRTSGYLPGPD
                     DIYVSMSQVRKHQLRKGDVVTGKVRQPRDNEKYWALLEVEKVNDMDPEASKERSNFDK
                     LTPLFPDERFRLESGPTAVTERIIDMIAPIGKGQRGMVVSPPKAGKTTVLKQIANGII
                     NSSPDTHVIVLLVDERPEEVTDWQRTVEAAEVVYSTFDKPTDQHIQVTELVLERSRRL
                     AESGKDVAVILDSITRLARAYNLAAPSSGKILSGGIDSGALYPPRRFFGA"
     gene            complement(1366..2067)
                     /gene="fsa"
                     /locus_tag="VF036_01870"
     CDS             complement(1366..2067)
                     /gene="fsa"
                     /locus_tag="VF036_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX6264007.1"
                     /translation="MRLFLDTADIEEIREINRWGVLSGVTTNPTLVVKEGGDPEAVWK
                     EILAEVEGDISLETTEPEAGPMYEEGLRLAGMAPNAVVKVPMTPAGLETGKRLGGDGI
                     RTNVTLVFSPAQAILAAEIGAYCVSPFLGRLDDVGADGMKLLHDICEIFAVQGYTSNV
                     LAASLRHPMHVVDAALAGADIATMPYDVFTKLVKHPLTDSGLERFLADWRTVGGEQPA
                     ADGPDRPDTNTNQQP"
     gene            complement(2064..3002)
                     /locus_tag="VF036_01875"
     CDS             complement(2064..3002)
                     /locus_tag="VF036_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264008.1"
                     /translation="MTRDKTPHITISDRDGLPYSKGLMASQVMVTGLSVYRSYEVAET
                     IELRLLDAGRSSVASSELSDLALMVIQELAGDRYATNFGRWRDVERLDVPLVILIGGA
                     TGVGKSTIATHLAHRLGIVRVVATDGSIREVMRSMLSAELMPTLHVSSFQTDTVVREP
                     TRRSDALVAGFREQTAAVSVGIEALITRAAADGTSIVIEGVHVVPGFFDPAAHGDHIV
                     PVPLIVEVEEESRHLHHFAARETGVRPAERYANNFGNIRKLQRYIKSQALSHGVPIVP
                     NHSFDQAISAVLDLVMDRATARIAQHAELGQQGRTA"
     gene            complement(3010..>3632)
                     /locus_tag="VF036_01880"
     CDS             complement(3010..>3632)
                     /locus_tag="VF036_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HEX6264009.1"
                     /translation="VIVEGSGSAIPPVPWDAAILVVPATAPPEYLRGYLGPYRLLRSD
                     LVVVTMASDPSGSENLSALRSPVRRYLNDAAFIVTNFVPVPLEEIRGKEAFFATTAPP
                     AVVEESIRRLEADHGCSVVGWSARLADRAGLVEDLDAVQGYDVLLTELKAAAVDTAVA
                     RALDRGAEVVFVDNRAEAAEGSVDLDTALGGVIDLALDRGSTRLTS"
BASE COUNT          516 a         1262 c         1222 g          632 t
ORIGIN      
        1 cgcaccgaag aaccggcgag gcgggtagag ggcgcccgag tcgatcccgc ccgacaggat
       61 cttgccagac gagggcgcag ccaggttgta ggcccgcgcc agacgggtga tcgagtcgag
      121 gatgaccgcg acgtccttgc ccgattcggc gaggcgccgc gaccgctcga gcacgagctc
      181 cgtgacctgg atgtgctggt ccgtgggttt gtcgaacgtc gagtacacga cctcggcggc
      241 ttcgaccgtc cgttgccagt ccgtgacctc ctcgggccgt tcgtccacga gaaggacgat
      301 cacgtgcgtg tccgggctcg agttgatgat cccgttcgcg atctgcttga ggacggtggt
      361 cttccccgcc ttgggcggcg acaccaccat gccgcgctgt cccttgccga tgggagcgat
      421 catgtcgatg atccgctcgg tcacggccgt gggaccggac tcgagccgga accgctcgtc
      481 ggggaacagt ggcgtcagct tgtcgaagtt gctccgctcc ttcgacgcct ccgggtccat
      541 gtcgttgacc ttctcgacct cgaggagcgc ccagtacttc tcgttgtccc gcggctggcg
      601 caccttgccc gtcacgacgt cccccttgcg gagctggtgc ttgcgcacct gggacatcga
      661 gacgtagatg tcgtcggggc ccggcaggta gcccgacgtg cgcatgaagc cgtagccgtc
      721 ggggagcagg tcgtggatcc cagaggcgga gggggcggtc ttcagcgctt cgatgcgctc
      781 ctgctcctcc cgctcccggc gctcctcgcg catctgccga cgctcttcgc gggtggggcg
      841 acgacgacgg ttccgcggat cgttccgatc cccggagtcc cctcggtcgt cacgatcgcc
      901 gccttgcggc cgcgatccgc cacggccccc ctgctgccgg tcgtccctgg ggcggtcctc
      961 accgccgggt cgccgatcgt cccggcgcgg ggacgcggtc tcgaccgacg cggccgaacg
     1021 gtcgttcacg ccgtccgaag gctgggactc gtctccgtcg tctccgtcgt ctccgttgtc
     1081 tccctcatcg gcgtcgcgca cactcgcggg agcgcgctcg accgtcgcga cagcgctctc
     1141 gacggaggca gcggatctcg cctccccgtt cccgggtcgc tcgccgttgg acgaggcctc
     1201 gacgatcgcg tcgatgaggc ccgccttgcg caggcgttgg gttccttcga tgcccatcgt
     1261 ctgtgcgatc tgctgcagct cggggaggag cttcccctcc agcgtgctgc ggtccatggt
     1321 ttccatcggt gccctgcctt tctttctcgc gagggccttg gctcgctagg gctgttggtt
     1381 cgtgttcgtg tcgggccggt cgggtccgtc cgcggcgggc tgttcgcccc cgaccgttcg
     1441 ccaatcggcg aggaaccgct cgagcccgga gtcggtcaag gggtgcttca cgagcttcgt
     1501 gaagacgtcg tagggcatcg tcgcgatgtc cgcgcccgcg agcgcggcat ccacgacgtg
     1561 catcgggtga cggagcgacg cggcgaggac gttcgaggtg tagccctgca ccgcgaagat
     1621 ctcgcagatg tcgtgcagca gcttcatccc gtccgcgccc acgtcgtcga gccgtccgag
     1681 gaagggtgag acgcagtacg cgccgatctc ggcggcgagg atcgcctgcg ccggcgagaa
     1741 gacgagcgtc acgttcgtgc ggatcccgtc gccgccgagc cgcttgccgg tctcgagccc
     1801 cgccggggtc atcgggacct tgacgaccgc gttcggcgcc atgccggcca gccgcagtcc
     1861 ctcctcgtac atgggacccg cctcgggttc ggtcgtctcg agcgagatgt cgccctcgac
     1921 ctccgcgagg atctccttcc agaccgcctc gggatcgccg ccttccttca cgaccagcgt
     1981 cgggttggtg gtgaccccgg acagcacgcc ccaacgattg atctcgcgga tctcctcgat
     2041 gtcggccgtg tccaggaaca gtctcacgcc gtcctcccct gttgtccgag ctccgcgtgc
     2101 tgggcgatcc tcgccgtcgc gcgatccatc acgaggtcga gcacggccga gatcgcctgg
     2161 tcgaagctgt ggttcggcac gatcggcacg ccgtgcgaga gcgcctggct cttgatgtag
     2221 cgctggagct tcctgatgtt gccgaagttg ttcgcgtacc gctcggcggg ccgtacaccc
     2281 gtctccctgg cagcgaagtg gtgcaggtgt cggctctcct cttccacctc gacgatcaac
     2341 ggcaccggca cgatgtgatc tccatgcgcg gcggggtcga agaacccggg gacgacgtgc
     2401 acaccttcga tgacgatgct cgtgccgtcg gcggccgccc gcgtgatcag cgcctcgatc
     2461 ccgaccgaga ccgccgccgt ctgctcccgg aacccggcga cgagcgcgtc ggaacgtcgc
     2521 gtcggttccc tgacgaccgt gtccgtctgg aacgaggaga catggagcgt cggcatgagc
     2581 tcggcggaga gcatcgagcg catgacctcc cggatcgagc cgtcagtggc gacgacgcgc
     2641 acgatcccca gccggtgcgc caggtgcgtg gcgatcgtgg acttcccgac cccggtcgct
     2701 ccgccgatca ggatcacgag cggcacgtcg agccgctcca cgtcccgcca ccggccgaag
     2761 ttggtggcgt agcgatcgcc cgccagctcc tggatgacca tgagggcgag gtccgagagc
     2821 tcggagctcg cgaccgacga ccgtccggcg tcgagcagtc gcagctcgat cgtctccgcc
     2881 acctcgtacg agcggtagac cgacaatccc gtgaccatga cctgggaggc catcagtccc
     2941 ttcgaatagg gaaggccgtc gcgatcggag atcgtgatgt gcggtgtctt gtctctcgtc
     3001 atcggcctct catgaggtga ggcgggtgct gccccggtcg agcgcgaggt cgatcacccc
     3061 gccgagcgcg gtgtcgaggt cgacggatcc ctccgcagcc tctgcgcggt tgtccacgaa
     3121 cacgacctcc gcccctcggt cgagggccct ggcaaccgcc gtgtcgacgg ccgccgcctt
     3181 cagctccgtg aggaggacgt cgtatccctg gacggcatcg aggtcttcga ccaggccggc
     3241 gcgatccgcc agccgcgcgc tccaacccac gacactgcac ccatgatcgg cttcgagacg
     3301 tctgatcgac tcctcgacga ccgcaggagg tgcagtcgta gcgaagaacg cttccttgcc
     3361 ccggatctct tccagcggca ccggaacgaa gttcgtcacg atgaacgccg catcgttcag
     3421 gtagcgccga acaggggaac ggagcgcgga aaggttttcg gacccggatg ggtcgctcgc
     3481 catggtaaca accaccaggt cggaccgcaa gaggcggtag gggcccaggt agccgcgcag
     3541 gtattccggg ggggccgtgg cggggacgac gaggatcgcc gcgtcccagg ggaccggcgg
     3601 gatcgccgac ccggacccct ccacgatgac ga
//
LOCUS       DASUVE010000091         3489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_422114, whole genome shotgun sequence.
ACCESSION   DASUVE010000091 DASUVE010000000
VERSION     DASUVE010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422114"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /gene="topA"
                     /locus_tag="VF036_01885"
     CDS             complement(<1..1704)
                     /gene="topA"
                     /locus_tag="VF036_01885"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX6264010.1"
                     /translation="MPGTLVVVESPAKAKTIEKYLGGDYAVRASFGHVRDLPKSQLGV
                     DVDDGFEPVYEVPEDSKRHVAELKRALKGADELVLATDYDREGEAIAYHVATLLGVAP
                     DQAKRITFTEITRDAILESVRHPRAIDLKLVDAQEARRVLDRLVGYRISPILWRKVRP
                     GLSAGRVQSVAVRLIVEREREIRAFVPVEYWSVDVRLTPEGDEQPFVARLTEIPEGKL
                     ATAPDKKGILLGAEADAMPHVERLGRASYRVVDVQRKERKRSPAPPFTTSTLQQEAAR
                     KLGFSARKTMTLAQRLYEGISLPGEGSVGLITYMRTDSVTIADAALREIAELVKQEYG
                     PSYALAEHRRYKTRSRNAQEAHEAVRPTSVLRSPDRVAASLDRDQLRLYDLIWKRTMA
                     TQMAEARFAQVGVDIRAVDGDADYGLRATGQTLVFDGFIRVYTEGRDEGPDEDAESRL
                     PELSAEQVLRMLELLPEQHFTQPPPRYTEASLVKTLEELGIGRPSTYASIIQTIQARG
                     YVRLDQRRFVPEDVAEVVTDKLIQHFPDVVDVNFTARMEEELDEIAEGNATRVAVLRD
                     FY"
     gene            1806..2564
                     /locus_tag="VF036_01890"
     CDS             1806..2564
                     /locus_tag="VF036_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX6264011.1"
                     /translation="MPAERGDPAPETARTRWYAADIPGPGGCSPLLETLQEAITTLIG
                     DYGYVAIFVLMVLESACIPIPSEVTMLFGGALASPGFAAPGHELDLVVVALMGTLGNL
                     VGSWLAYGIGAAGGRPLVDRFGRYLLILPHEVDRAHDWFERRGELTVFVTRLLPVIRT
                     FISLPAGVARMPFWRFTIYTVLGCLPWTFALAWLGYLLGENWAKVEEFLQPVAWGIVV
                     VLAIGLVWYVARRYRRVRAAYAELDAASGGGEAG"
     gene            2574..>3489
                     /locus_tag="VF036_01895"
     CDS             2574..>3489
                     /locus_tag="VF036_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6264012.1"
                     /translation="MRACSSCGRQNPDDARFCAHCGSALVEVPGSAREERKVITVLFA
                     DLVGFTSRSEQLDPEDVRATLTPYFARLREELERRGGTVEKFIGDAVMAVFGAPTTHE
                     DDPERAVRAALAIRDALAEMNEQDPGIDLHVRIGVNTGEALVSLGVRPLEGEGMAAGD
                     VVNTAARLQTSAPVDGVLVGDATYRATERAIEYRVGDPVEAKGKADPIGVWEAIEARS
                     HYGVDITRRVDTALVGRDRELALLRDALDRARHQNEPQLVTLVGEPGIGKSRLVHELL
                     VHIEDMPDLITWRQGRCLPYGDGVSYWAL"
BASE COUNT          492 a         1190 c         1235 g          572 t
ORIGIN      
        1 gtagaagtcg cgcaggacgg ccacgcgggt ggcgttgccc tccgcgatct catccagctc
       61 ctcctccatc cgggcggtga agttgacgtc gacgacgtcg gggaagtgct ggatcagctt
      121 gtccgtcacc acctcggcca cgtcctccgg cacgaaccgc cgctggtcca ggcggacgta
      181 gccccgcgcc tggatcgtct ggatgatcga cgcgtacgtc gagggccgac cgatgcccag
      241 ttcctcgagg gtcttgacga gcgacgcttc cgtgtaccgg ggcgggggct gggtgaagtg
      301 ctgctcgggc agcagctcga gcatccgcaa cacctgctcc gcggagagct cgggcaggcg
      361 cgactcggca tcctcgtcgg gtccctcgtc tcgtccctcg gtgtagaccc ggatgaaccc
      421 gtcgaacacg agcgtctgtc cggtcgcccg cagaccgtag tccgcgtccc cgtcgacggc
      481 tcggatgtcc acgccgacct gggcgaagcg agcctcggcc atctgcgtcg ccatggtgcg
      541 cttccagatc aggtcgtaca ggcggagctg gtcgcggtcc agcgaggcgg cgacccggtc
      601 gggggaacgg aggaccgatg tggggcggac ggcctcgtgg gcttcctgag cgttgcgcga
      661 ccgggtcttg tagcgccggt gctcggcgag cgcgtacgaa gggccgtact cctgcttcac
      721 gagctccgcg atctcgcgga gggccgcgtc ggcgatggtc accgaatcgg tccgcatgta
      781 cgtgatcagt cccaccgacc cctcgccggg aagcgagatg ccctcgtaca ggcgctgcgc
      841 gagcgtcatc gtcttgcgcg ccgagaaccc gagcttccgt gccgcctcct gctgcagggt
      901 ggaggtggtg aacggcgggg ccggcgagcg cttgcgctcc ttccgctgga cgtcgacgac
      961 gcggtaggag gcccggccga gccgctccac gtgcggcatc gcgtcggcct cggcgccgag
     1021 gaggatgccc ttcttgtccg gcgcggtcgc gagcttgccc tcggggatct ccgtgagccg
     1081 agcgacgaac ggctgctcgt cgccctccgg cgtgagccgg acatcgacgc tccagtactc
     1141 gacgggaacg aaggcgcgga tctcgcgctc gcgctcgacg atcaggcgaa cggcgaccga
     1201 ctgcacccgc cccgcggaca gcccgggccg caccttcctc cagaggatcg ggctgatccg
     1261 atagccgacg agacggtcga ggacccggcg ggcttcctgg gcatcgacga gcttcaggtc
     1321 gatcgcgcgc gggtgccgca ccgactcgag gatcgcatcc cgcgtgatct ccgtgaacgt
     1381 gatcctcttc gcctggtcgg gcgcgacgcc gaggagggtg gccacgtggt acgcgatcgc
     1441 ttcgccctcc cggtcgtagt cggtggcgag cacgagctcg tccgcaccct tgagcgcgcg
     1501 cttcagctcc gcgacgtgcc gcttggagtc ctcggggacc tcgtagacag gttcgaatcc
     1561 gtcgtccacg tcgacgccga gctgactctt cggcaggtcc cggacgtgcc cgaaggaagc
     1621 ccggacggcg tagtcgccgc ctaggtactt ctcgatggtc ttcgccttgg cgggcgattc
     1681 gacgacgacg agggttcctg gcatatcgaa cgtggcttcc ttggggggtt gggtggagtc
     1741 aggtcgagcc tttcggaccg taccactggc ctgtcgaagc tcctgggagg cgcggatgtg
     1801 cagggatgcc ggccgaacga ggcgaccccg ctcccgagac ggccagaaca cgctggtacg
     1861 ctgcggatat tcccggcccc ggagggtgct ctcccctgct cgagacgctg caagaagcca
     1921 tcaccacgct gatcggcgac tatggctacg tcgccatctt cgtcctgatg gtgctcgagt
     1981 ccgcgtgcat cccgatcccg tccgaggtca ccatgctgtt cgggggcgcc ctcgcgagcc
     2041 ctgggttcgc ggcccccggt cacgagctcg atctcgtggt ggtcgccctc atggggacgc
     2101 tcggcaacct cgtcggctcg tggctggcct atggcatcgg cgcggcaggc ggccgcccgc
     2161 tcgtggaccg gttcggccgg tacctgctga tcctgcccca cgaggtggat agggcgcacg
     2221 attggttcga gcggcgcggc gagctcacgg tcttcgtcac ccggttgctc ccggtgatcc
     2281 gcacgttcat ctcgcttccc gccggcgtcg cacggatgcc gttctggcgc ttcacgatct
     2341 acacggtgct cgggtgcctg ccgtggacgt tcgcgctcgc gtggctcggg tacctgctcg
     2401 gtgagaattg ggcgaaggtg gaggagttcc tgcagcccgt cgcgtggggg atcgtggtgg
     2461 ttctggcgat cggtctggtc tggtacgtgg cgcgtcgcta ccggcgggtg cgagccgcct
     2521 acgccgagct cgacgccgcc tccggaggcg gagaggcggg gtaggctccc gccgtgcgcg
     2581 cctgctccag ctgcggacga cagaaccccg atgacgcacg gttctgcgcc cactgcggct
     2641 cggcgctcgt cgaggtaccc ggttcggcgc gtgaagagcg gaaggtcatc accgtgctct
     2701 tcgctgacct cgtgggcttc acgagcagga gcgagcagct cgaccccgaa gacgtgcgcg
     2761 cgacgctcac tccctacttc gcgcgcctcc gggaggagct ggaacgccgc ggcggaaccg
     2821 tcgagaagtt catcggggac gcggtgatgg ccgtgttcgg cgccccgacc acgcacgagg
     2881 acgaccccga acgggcggtc cgagccgcgc tggcgatccg cgacgcgctt gcggagatga
     2941 acgaacagga cccgggcatc gatctccacg tccgcatcgg cgtgaacacc ggcgaggcgc
     3001 tcgtctcgct cggcgttcgc cctctcgagg gggaagggat ggccgccggc gacgtagtga
     3061 acacggcggc caggctccag acatccgccc ccgtcgacgg ggtgctggtc ggcgacgcga
     3121 cctatcgcgc aaccgagcgg gcgatcgagt accgagtggg cgatcccgtc gaggcgaagg
     3181 gcaaagccga cccgatcggg gtctgggagg cgatcgaggc ccgctcccac tacggggtcg
     3241 acatcacccg ccgcgtcgac accgcgctcg tcggtcgcga tcgcgagctc gcgctcctcc
     3301 gcgacgcgct cgatcgcgcg cgacatcaga acgagccgca actcgtcacg ctcgtcggcg
     3361 agcccgggat cgggaagagc cgtctcgtgc acgagctcct cgtccacatc gaggacatgc
     3421 cggacctgat cacctggcgg cagggccggt gcctgcccta cggcgacggg gtgagctatt
     3481 gggcgctcg
//
LOCUS       DASUVE010000092         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1363768, whole genome shotgun sequence.
ACCESSION   DASUVE010000092 DASUVE010000000
VERSION     DASUVE010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363768"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VF036_01900"
     CDS             <1..925
                     /locus_tag="VF036_01900"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX6264013.1"
                     /translation="LEAWMRRGADRKDLVLYLETFAHTVGVMQTTESLARVARECAED
                     LAADGIVYAEIRYAPEQHLERDLTLDEVVEAVQRGFVEGSQTTGIRIGTLLTAMRTAA
                     RSLEIAELAVRWRDRGVVGFDIAGAEAGYPPTRHLDAFDHVRRANFHLTIHAGEAFGL
                     PSIWEALQLCGAERLGHGVRIVDDIEVDPDGEARLGRVADLVRDRRVPLELCPTSNVH
                     SGAAASIGEHPIGLLADLKFRVTVNTDNRLMSGTTLSNEFAQLVEAFGIGWDRIRRLT
                     INAMKSAFLPFDERVIFLDDVIRPAYLEHDR"
     gene            922..1413
                     /locus_tag="VF036_01905"
     CDS             922..1413
                     /locus_tag="VF036_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX6264014.1"
                     /translation="MTAERDATKDTPAIREVAASDVPFRVVRTERPGSAEESAEFQGI
                     ELGQLIRSIVVRRGAGDYIFVLVPGGRQIAWARLRAHLGVSRLSLPDQDEARDATGYE
                     RGTITPFASSSRWPVVADATLADRHVVAIGAGAHGVNLHLDPADLFRLVHADLADVTD
                     PAG"
     gene            complement(1454..>2083)
                     /gene="pgl"
                     /locus_tag="VF036_01910"
     CDS             complement(1454..>2083)
                     /gene="pgl"
                     /locus_tag="VF036_01910"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX6264015.1"
                     /translation="AEALAARAAAVIEAAAGGAVADRGTFRWAVSGGETPVPTFRRLA
                     LPWPATGTFQVDERVAPEGDDDRNLTHLRRALRADGAATVRPMPVEADDLESAAAAYE
                     RSLPERFDVIHLGIGDDGHTASLLPDDPVLEVRDRDVAVTGTFRGRRRMTLTFPVLDR
                     ARRVLWIVAGSTKADALARLLARDTSIPAGRVMAADQLVLADADAAPPP"
BASE COUNT          292 a          738 c          759 g          294 t
ORIGIN      
        1 gctcgaggcc tggatgaggc ggggggccga tcgcaaggat ctggtcctgt acctcgagac
       61 gttcgcccac accgtggggg tgatgcagac caccgagtcg ctggcacgcg tcgcccggga
      121 gtgtgcggag gacctggccg ccgatgggat cgtctacgcc gagatccgct acgccccgga
      181 gcagcacctc gagcgggacc tcaccctgga cgaggtcgtc gaggcggtgc agcggggatt
      241 cgttgagggc tcccagacga ccgggatccg gatcggcaca ctgctcacgg cgatgcgcac
      301 ggccgcgcga tcgctcgaga tcgcggagct cgccgtccgc tggcgggacc gcggggtggt
      361 gggcttcgac atcgcgggcg cggaggccgg gtacccgccg acccgtcacc tcgatgcgtt
      421 cgaccacgtc cgccgcgcga acttccacct cacgatccac gcgggagagg cgttcggact
      481 gccgtcgatc tgggaggccc tgcagctgtg cggcgccgag cggctcggcc acggggttcg
      541 catcgtcgac gacatcgagg tcgatccgga tggcgaggcc cggctcggac gcgtcgccga
      601 tctcgtccgt gaccggcgtg tgccgctcga gctgtgcccg acgtccaacg tccactccgg
      661 agcggcggcc tcgatcggtg agcacccgat cgggctcctg gcggatctga agttccgcgt
      721 caccgtgaac accgacaacc ggctgatgtc ggggacgacc ctctcgaacg agttcgcaca
      781 gctcgtggag gcgttcggga tcggatggga caggatccgc cggctcacga tcaacgcgat
      841 gaaatcggcg ttcctcccgt tcgacgagcg cgtcatcttc ctcgacgacg tgatccggcc
      901 ggcatacctc gaacacgatc gatgacggcc gaacgggatg caacgaagga caccccggcg
      961 atccgtgagg tggccgcgtc ggacgtgccg ttccgcgtgg tccggacgga gcggccgggc
     1021 agtgccgagg agagcgccga gttccagggc atcgagctgg gacagctgat ccggtcgatc
     1081 gtggtgcgac gcggcgccgg cgactacatc ttcgtcctcg tgcccggcgg gaggcagatc
     1141 gcctgggcgc ggctccgcgc gcacctcggg gtgagccggc tgtccttgcc ggaccaggac
     1201 gaggcccgcg acgcgaccgg ctacgagcgg gggacgatca ccccgttcgc gtcgtcgtcg
     1261 cgctggccgg tcgtggccga cgcgaccctc gccgatcgcc acgtggtcgc gatcggcgcc
     1321 ggcgcgcacg gcgtcaatct ccacctcgat ccggcggatc tgttccggct cgtgcacgca
     1381 gatctcgcgg acgtgaccga ccccgccgga tgagtgtcgc cgggctccag gcgcgccgtg
     1441 atcacggagc gggtcaaggg ggcggcgccg cgtcggcgtc tgccagcacc agctgatcgg
     1501 ccgccatcac gcgtccggcc gggatggacg tgtcacgtgc gaggaggcgg gccagcgcgt
     1561 ccgccttcgt gcttcccgcg acgatccaca ggacccgccg agcccgatcg agtaccggaa
     1621 aggtgagggt catccgtcgc cgtcctcgga acgttcccgt caccgcgacg tctcgatcac
     1681 gcacctcgag gacggggtcg tcgggcagga gggacgccgt gtgcccgtcg tcgccgatcc
     1741 cgaggtggat gacgtcgaac cgttcgggca gcgaccgctc gtaggccgcc gccgcggact
     1801 cgagatcgtc ggcctccacc ggcatcgggc gcacggtggc cgccccgtcg gcccgcaggg
     1861 cccggcggag atgcgtcagg ttccggtcgt cgtccccctc gggggcgacc cgctcgtcga
     1921 cctggaacgt cccggtcgcg ggccagggca acgcgagccg gcggaaggtc gggacgggcg
     1981 tctccccgcc gctgactgcc cagcggaagg tcccgcgatc ggccaccgct ccccccgcgg
     2041 ccgcctcgat caccgccgcc gcccgggccg cgagggcctc ggc
//
LOCUS       DASUVE010000093         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1552525, whole genome shotgun sequence.
ACCESSION   DASUVE010000093 DASUVE010000000
VERSION     DASUVE010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552525"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VF036_01915"
     CDS             <1..703
                     /locus_tag="VF036_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6264016.1"
                     /translation="LVEDHLDVFFESNVWHVRGRDRDLVVDTGNGIGDLRGELAPLVE
                     DRPTVAVATHDHFDHIGGMHAFEERWCHAADAEGLREPEGLALLRRDYRPGLEDEIRW
                     YGYEPPESVITAVPGPTFDVGGWRTSPAEPTRVLADGDVVDLGDRSFEVLHVPGHTAG
                     SIALWDAEAGLLFTGDTAALDDPLYADDEAAFVASLERLSALPVELVCAGHSRPFGRE
                     ELSALIDEQLASRRP"
     gene            complement(713..2260)
                     /locus_tag="VF036_01920"
     CDS             complement(713..2260)
                     /locus_tag="VF036_01920"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HEX6264017.1"
                     /translation="MVRRKHHASIVAGSALVILASLFGAVSLPAAAVEPTWFDLPASD
                     SFPQGLAAGPDGMTWVASRAAAEIARLAPDGSSSSIPLESGVDPFDIVRGPDGAMWFT
                     EQNGNRIGRLTMEGELSEFYLRDLSTPMGITVGPDGAIWFAQRGVNAIGRITSDGELT
                     EWGTLTPRGAPLSVTTGSDGAMWFTLTSADRIGRITLDGTMAEFPLPADASGPRWITT
                     GPDGALWFTARASNTIVRMTTGGAVTTFPVPTPGAGVSGITAGADGALWFTEGTADAV
                     GRIGLDGAIVEIPLGEGASPTGITTGPDGAIWFSAQGTNRVGRLEPSGPSDRAAPTVT
                     IVSPAHGSVLTDGESMVADYFCTDEAGGSGVVSCQGPVADGAIVPNRPGSHAFTVTGT
                     DAEGNVGSATHGYVVFEDISGPITNKAVFSAGRVIPIILELGGHPQGSVFANGYPLVR
                     KVHCATGEVLAEHAASVQADLSNNGRLMVQWRTEAGWAASCRSLVVRLGLGGWSDADA
                     VFTVRFA"
BASE COUNT          325 a          815 c          807 g          340 t
ORIGIN      
        1 gctcgtcgag gatcacctcg acgtcttctt cgaatcgaac gtctggcacg tccgtggacg
       61 cgaccgcgac ctcgtggtgg atacaggcaa cgggatcggc gatctgcgcg gtgagctcgc
      121 ccctttggtc gaggaccggc cgaccgtggc ggtcgcgacc catgaccact tcgaccacat
      181 cggggggatg cacgcgttcg aagagcgctg gtgtcatgcg gccgacgcgg aggggctgcg
      241 ggaacccgaa ggtctcgccc tcctccggcg ggactaccgg cccggtctgg aggatgagat
      301 ccgctggtac ggctacgagc cgcccgagag cgtgatcacg gcggtgcccg gtccgacctt
      361 cgacgtcggc ggctggcgga cgtcgcccgc ggagccgacg cgcgtcctcg cggacggcga
      421 cgtcgtggat ctgggggacc gctctttcga ggtcctccac gtcccgggcc acaccgcggg
      481 ctcgatcgcg ctgtgggacg cggaggccgg tctgctgttc acgggcgaca ccgccgctct
      541 cgacgaccct ctgtacgccg acgacgaggc ggcgttcgtg gcgtcgctcg agcggctctc
      601 ggcgctgccg gtcgagctcg tctgcgcggg gcactcccgg ccgttcgggc gggaagagct
      661 cagtgccctt atcgacgagc agctcgcgtc gcgacgcccc tgacgcgcgg cgtcaggcga
      721 agcgcacggt gaacacggcg tcggcgtccg accagccgcc gagcccgaga cggaccacga
      781 gcgagcggca cgaggcggcc cagcccgcct ccgtccgcca ctgcaccatc agtcgaccgt
      841 tgttcgacag atccgcctgg accgacgccg cgtgctcggc caggacctcg ccggtcgcgc
      901 aatgcacctt gcgcaccagc gggtacccgt tcgcgaacac ggatccctgg gggtgtccgc
      961 cgagctcgag gatgatcggg atcacacgcc ccgccgagaa gaccgccttg ttcgtgatcg
     1021 gcccgctgat gtcctcgaac acgacgtacc cgtgggtcgc ggagccgacg ttcccctccg
     1081 cgtccgtgcc ggtgacggtg aacgcgtgcg accccggccg gttcgggacg atcgcgccgt
     1141 ccgcgacggg tccctgacag gagaccaccc ccgacccccc cgcctcatcc gtgcagaagt
     1201 agtcggcgac catactctcg ccgtcggtca gcaccgagcc gtgagccggc gatacgatcg
     1261 tgacggtcgg agcggcccta tcgctggggc cggacggctc gagccgcccc acgcggttcg
     1321 tgccttgggc gctgaaccag atcgcgccgt ctggcccggt cgtgatgccg gtcggtgagg
     1381 cgccttcgcc gagcgggatc tcgacgatcg cgccgtcgag gccgatccgg ccgacggcgt
     1441 cggcggtgcc ctcagtgaac cagagggcgc cgtcggcccc ggccgtgatc ccgctcaccc
     1501 cggctcccgg cgtggggacc gggaacgtcg tgacggcgcc ccctgtcgtc atccgcacga
     1561 tcgtgttgga cgcgcgagcc gtgaaccaca gcgcgccgtc cgggccggtg gtgatccatc
     1621 gcgggccgct cgcgtccgcg gggagcggga actccgccat cgtgccgtcg agcgtgatac
     1681 ggccgatcct gtctgccgag gtcagggtga accacatcgc tccgtcggat cccgtcgtga
     1741 cgctgagcgg cgcgccccga ggggtgagcg ttccccattc ggtgagctcc ccatccgatg
     1801 tgatgcggcc gatcgcgttg acgcctcgct gcgcgaacca gatcgcgccg tcggggccga
     1861 ccgtgatgcc catgggcgtc gacaggtcgc ggaggtagaa ctcggagagt tccccctcca
     1921 tcgtgagccg cccgatccgg ttcccgttct gctcggtgaa ccacatcgcc ccatcgggcc
     1981 ctcggacgat gtcgaagggg tcgacgccgg actcgagggg gatcgacgac gacgatccgt
     2041 caggagcgag ccgcgcgatc tcggcggccg cccggctggc gacccacgtc atcccgtccg
     2101 gtccggcggc gagtccctgt gggaaggagt ccgacgctgg caggtcgaac cacgtgggtt
     2161 cgacggccgc ggcgggcagg ctgacggcgc cgaacaacga ggcgaggatc accaacgcgg
     2221 accccgccac gatcgaagca tggtgcttgc gccgtaccat gtttcgggta tcggcatctc
     2281 ggatcga
//
LOCUS       DASUVE010000094         2402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_233825, whole genome shotgun sequence.
ACCESSION   DASUVE010000094 DASUVE010000000
VERSION     DASUVE010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233825"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VF036_01925"
     CDS             <1..1077
                     /locus_tag="VF036_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX6264018.1"
                     /translation="DLAAEVATEILTILGAEIRLVRSEEGRPSVVARFGSGARPRLAW
                     NGHLDTVPTGNASRWSSGPFDGEVVNGRLVGRGACDMKGPIASAIGAIAGIRRAGIGL
                     AGTLELHLVADEEHAGVHGTRVLRDEGLLDQDAAIVGEPTEMEIALAERGGAWVTAVA
                     HGKAAHGSQPHRGVNAILTMSRFLLRLPEVIPDLIHPLVGAPTVNVAMVAGGSAPNVV
                     PDRCEAEIDRRIVPGEDDRDAVLAPFHRLVGDLVEEQRDTSIEIILREWTEAAETTGD
                     SAIASIVRDAIAADTGAPPPFVGFTGITDARFYINDARIPTVIAGPGSLSVAHAADES
                     IGVEEMVTAARSYARMFVAYLGTV"
     gene            1082..>2402
                     /locus_tag="VF036_01930"
     CDS             1082..>2402
                     /locus_tag="VF036_01930"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6264019.1"
                     /translation="MDSVDVFVIGGGGTGSEVAFSLARSSGLSVALAERDKLGGECNH
                     YGCVPTKVMLRSARIAKLARDAERFGLRIPDVSVDFPAVRQRIRDVIAAQSGEGAAPF
                     ERLGVRMFLQEARLVGEHRVELADGSVLEAGKVVLATGTHAAVPPIAGIEGGPFWTNR
                     EAIWEPATPPRSLAVIGTGAVGIEFAQIYARFGTHVTALEVLPHVLPQEDEEAAASIL
                     PALEAEGITMRSGVRIEGARHDGTAWTLAIEGGAPVIADELLVATGRRPVFEPHDLAA
                     AGIELDDEGNPVLSETLRTRNPDVWVAGDATGDLLFTHVGSYEAELVVKDVVGHPEAR
                     DYRVVPRVTFCDPEVASVGLTEQQARDDGRDVITSVMRFDDNERARIDGSTHGLVKLV
                     ADRKTGELLGGHIVCEEAGALIHEIVVLMAARTPATAVHDAIHAYPTL"
BASE COUNT          356 a          830 c          873 g          343 t
ORIGIN      
        1 gacctggcgg ccgaggtcgc caccgagatc ctcaccatcc tcggagcgga gatccggctc
       61 gtgcgctccg aggagggccg ccccagcgtc gtcgcccgct tcgggtcggg tgcccgaccc
      121 cgtctggcgt ggaacggcca cctcgacacc gtccccaccg ggaacgcgtc gaggtggtcg
      181 agcggcccat tcgacggcga ggtcgtcaac ggaaggctcg tggggcgcgg cgcctgcgac
      241 atgaaggggc cgatcgcgtc ggcgatcggt gcgatcgcgg ggatccgacg cgccgggatc
      301 gggctggcgg ggaccttgga gctccacctg gtcgccgacg aggaacatgc cggggtccac
      361 ggcacacggg tcctccgaga tgaaggcctg ctcgaccaag acgccgcgat cgtcggagag
      421 cccaccgaga tggagatcgc actggccgag cgcggcggcg cctgggtcac cgccgtggcg
      481 cacgggaagg ccgcccacgg ctctcagccg caccgcggcg tgaacgcgat cctcacgatg
      541 tcgcggttcc tgctccgtct cccggaggtg atcccggatc tcatccatcc gctcgtcggt
      601 gcccccacgg tcaacgtcgc catggtcgcc ggcggaagcg cccccaacgt cgtccccgat
      661 cgctgcgagg cggagatcga tcgtcggatc gtgcccggtg aggacgaccg cgatgcggtc
      721 ctcgccccct tccaccggct ggtcggcgac ctcgtcgagg agcagcggga tacgagcatc
      781 gagatcatcc tgcgggagtg gaccgaggcg gccgagacca caggggactc cgcgatcgcg
      841 tcgatcgtcc gagatgcgat cgccgccgac acgggcgcac cgccgccgtt cgtcggcttc
      901 accgggatca ccgatgcccg cttctacatc aacgacgcga ggatccccac cgtgatcgcg
      961 gggccggggt cgctgtccgt cgcgcacgcg gcggacgagt cgatcggggt cgaggagatg
     1021 gtcaccgcgg cacgctcgta cgcgcgcatg ttcgttgcgt acctcgggac cgtctagacg
     1081 cgtggactcc gtcgacgtct tcgtgatcgg gggtggcggc accggttccg aggtcgcgtt
     1141 ctcgctggct cgctcctcgg gactgtccgt cgcgctggcg gagcgcgaca agctcggtgg
     1201 ggaatgcaac cactacggct gcgtccccac gaaggtgatg ctccgatccg cgaggatcgc
     1261 gaagctcgcg cgggatgcgg agcgtttcgg tctccggatc cccgacgtct ccgtcgactt
     1321 ccccgcggtc cggcaacgga tccgcgacgt gatcgccgcg cagtcgggtg aaggcgcagc
     1381 gccgttcgag cggctgggcg tgcgcatgtt cctccaggag gcacggctgg tgggcgagca
     1441 tcgcgtggag ctggcggacg ggtccgtgct cgaggcgggc aaggtggtgc tcgccacggg
     1501 gacccacgcg gcggtccctc cgatcgccgg gatcgagggc gggccgttct ggacgaaccg
     1561 cgaggcgatc tgggagcccg cgacaccgcc tcgatcgctc gccgtgatcg ggacgggcgc
     1621 ggtggggatc gagttcgcgc agatctacgc gcggttcggc acacacgtca cggccctgga
     1681 ggttctcccc cacgtcctgc cgcaggagga cgaggaggct gccgcgtcga tccttcccgc
     1741 cctcgaggcc gagggcatca cgatgcgatc cggtgtgcgg atcgaaggag ccaggcacga
     1801 cggcacggcc tggacgctcg cgatcgaggg cggcgccccc gtgatcgccg acgagctgct
     1861 cgtggcgacg ggacgacgcc cggtgttcga gccgcacgat ctcgcggccg cgggcatcga
     1921 gctcgacgac gaagggaacc ccgtcctgtc ggagacgctc cggacccgca accccgacgt
     1981 gtgggtggcc ggggacgcca ccggcgacct gctcttcacc catgtcggct cctacgaggc
     2041 cgagctcgtg gtcaaagacg tcgtgggaca ccccgaggcg cgcgactacc gggtggtccc
     2101 acgcgtgacg ttctgcgacc cggaggtggc gagcgtgggc ctcaccgagc agcaagctcg
     2161 cgacgacggc cgagacgtga tcacctcggt catgcggttc gatgacaacg agcgcgcgcg
     2221 catcgacggg agcacgcacg ggctggtgaa gctggtcgcc gaccggaaga cgggcgagct
     2281 cctcggtggc cacatcgtct gcgaggaggc gggagcactg atccacgaga tcgtggtcct
     2341 gatggccgcg cggacgcccg cgaccgccgt ccacgacgcg atccacgcct atcccaccct
     2401 gt
//
LOCUS       DASUVE010000095         2788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_611065, whole genome shotgun sequence.
ACCESSION   DASUVE010000095 DASUVE010000000
VERSION     DASUVE010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611065"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VF036_01935"
     CDS             complement(<1..291)
                     /locus_tag="VF036_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HEX6264020.1"
                     /translation="MVNERPEMLAKSATLYFGPWYRQSPFFRSTLRAGCTAYDIYNHM
                     YLPGYFDDPEVEYHLLATGVTMWDVGVERTVQIAGPDADRLVDMVTCRDLSTC"
     gene            complement(423..1580)
                     /locus_tag="VF036_01940"
     CDS             complement(423..1580)
                     /locus_tag="VF036_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007205555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX6264021.1"
                     /translation="MTQPFDMSLVQRGARNRRTPYYEATQRYGPKGFTVYNHMYFPIR
                     FDTFEAEFDALLHGVTVWDVSVERCLEISGPDSFRFAQLLTPRDLSKCAVGQGKYVLI
                     CDSDGGIVNDPVLTRMDENTFWFALASSDALLFARGLKNAYPELDVTIREADVAPLQV
                     QGPKSKDLMAKLLGPEILDLKYYFWTEAKIADAPVVVTRTGWTSEIGYEVYLTDTSKG
                     TEVFEAIMEAGQEFGVVPTGPSDIRRIEGAIFNWGADMTYENNPLELGMDRLVAWDLS
                     DDASISIGALRRIRDEGVTQRINGVEFDGPPFPALNNVKWPAFDDGERIGKVTSAIHS
                     PRLGKNIGYCWLPAERSSSGTTVSVETEWGTRTATVAPMPFVDPDKTIPVS"
     gene            complement(1714..>2788)
                     /locus_tag="VF036_01945"
     CDS             complement(1714..>2788)
                     /locus_tag="VF036_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6264022.1"
                     /translation="AATPDDVYEGLLEGQRYLHSLGITAWQDAIVSDRFWLDNYSAYV
                     RAGESEALTARVIGALWWDRERGEEQIDQLVDLRARGREGRFAATSVKIMQDGVCETF
                     TAAVLEPYLDADGNVTDERGISFVEPEALKGYVTALDRLGFQVHFHALAERAVRESLD
                     AVEAALDANGPSDHRHHLAHLQVIHPDDVPRFRRLGAVANAPPLWAAHDGQMDDLTIP
                     FLGEPRWRWQYPFGSLVRSGAGLAMGSDWSVSSPDPLEEIHVAVNRVMPASYLYGNGN
                     ADVFLPDERLDLATALAAFTMGSAYVNHLDETTGSIEVGKFADLVVVDRDLFAHPVDE
                     IADARVDATFVEGVPVYERSGAS"
BASE COUNT          442 a          922 c          959 g          465 t
ORIGIN      
        1 gcacgtcgag agatcacggc acgtgaccat gtcgaccagc cggtcagcgt ccgggccggc
       61 gatctggacg gtccgctcca cgccgacgtc ccacatcgtc acgccggtgg cgagcaggtg
      121 gtactcgacc tcgggatcgt cgaagtagcc ggggagatac atgtggttgt agatgtcgta
      181 ggcggtgcag cccgcccgca gcgtcgatcg gaagaacggc gactggcggt accagggacc
      241 gaagtacagg gtcgcgctct tcgcgagcat ctcgggtcgt tcgttgacca tcgttcctcc
      301 tcgttctggc cggaagcagg ctgaatcatc gttcaagaag agcgtccgtg caagtcgccg
      361 tacgtcccct gaacaggaac gatgtggttg aacggtggtt aggtttcctc ggaccagcgg
      421 ggctacgaga ccgggatcgt cttatccggg tcgacgaacg gcatcggtgc cacggtagcc
      481 gtccgtgtac cccactcggt ttcgaccgac acggtggtgc ccgatgacga ccgctcggcg
      541 gggagccagc agtagccgat gttcttgccg agcctgggtg aatggatcgc cgacgtcacc
      601 ttgccgatcc gctcgccgtc gtcgaaggcg ggccacttca cgttgttcag cgccgggaac
      661 ggcgggccgt cgaactccac gccgttgatc cgctgcgtca cgccctcgtc gcggatcctg
      721 cggagcgcac cgatggagat cgaagcgtcg tccgagaggt cccatgcgac gagccgatcc
      781 atccccagct cgagcgggtt gttctcgtac gtcatgtcgg caccccagtt gaagatcgcg
      841 ccctcgatcc tccggatgtc gctcgggccg gtggggacga cgccgaactc ctgacccgct
      901 tccatgatcg cctcgaagac ctccgtgccc ttgctcgtgt cggtcaggta gacctcgtag
      961 ccgatctccg acgtccatcc ggtgcgcgtg accacgacgg gagcgtcggc gatcttcgcc
     1021 tcggtccaga agtagtactt caggtcgagg atctccgggc cgaggagctt cgccatcagg
     1081 tccttcgact tcggcccctg gacctgcagc ggtgcgacgt cggcctcgcg gatcgtcacg
     1141 tcgagctcgg ggtacgcgtt cttgaggccc cgcgcgaaca gcagggcatc cgaggacgcg
     1201 agcgcgaacc agaacgtgtt ctcgtccatg cgggtcagga ccgggtcgtt cacgatgccg
     1261 ccgtcgctgt cgcagatcag cacgtacttg ccctgaccca ccgcgcactt cgagaggtcg
     1321 cgcggcgtga ggagctgcgc gaaccggaag ctgtccggcc cggagatctc gaggcagcgc
     1381 tcgaccgaga cgtcccagac cgtgaccccg tgcaacagcg cgtcgaactc cgcctcgaac
     1441 gtgtcgaagc ggatcgggaa gtacatgtgg ttgtagacgg tgaagccctt cgggccgtac
     1501 cgctgcgtcg cctcgtagta cggggtccgg cgattgcggg ctccgcgctg cacgaggctc
     1561 atgtcgaagg gctgggtcat cgtggtggct cctctcgaag ggcgggccgc ctgctcgtcg
     1621 ccggcggtcg cgtctgcgca atcccgtccg tgggatgcct cggtatcgtc cgagggccgc
     1681 cagagcgagg caaccaggcg gggtgaccgg aagtcagctc gcgccggaac gctcgtagac
     1741 cgggacaccc tcgacgaacg tggcgtcgac acgggcgtcc gcgatctcgt cgaccggatg
     1801 ggcgaacagg tcgcggtcca ccacgacgag gtcggcgaac ttgcccacct cgatcgagcc
     1861 ggtggtctca tcgaggtgat tcacgtacgc cgagcccatc gtgaacgccg cgagcgcggt
     1921 ggcgaggtcg aggcgctcgt ccgggaggaa gacgtcggcg ttcccgttcc cgtagaggta
     1981 ggaggcgggc atcacacggt tcaccgcgac gtggatctcc tcgaggggat cggggctcga
     2041 gaccgaccag tcgctcccca tggccaggcc ggcaccggag cggaccaggg aaccgaaggg
     2101 gtactgccac cgccagcggg gctcccccaa gaacgggatc gtcaagtcgt ccatctgtcc
     2161 gtcgtgcgcc gcccacaggg gcggggcgtt cgcgaccgcc cccaggcgcc ggaaccgcgg
     2221 gacgtcgtcg gggtggatta cctgcaggtg ggcgaggtgg tggcggtggt cgctcgggcc
     2281 gttcgcgtcc aacgcggcct cgaccgcgtc gagcgattcc cgcacggcgc gctcggccag
     2341 cgcgtggaag tggacctgga agccgagtcg atccaacgcg gtcacgtagc ccttcagtgc
     2401 ctcgggctcc acgaaggaga tcccgcgctc gtccgtgacg ttgccgtcgg cgtcgaggta
     2461 cggctcgagg acggcggccg tgaacgtctc gcataccccg tcctgcatga tcttcacgct
     2521 cgtcgcggcg aagcgaccct cgcggccacg cgcgcggagg tcgacgagct ggtcgatctg
     2581 ctcctcgcct cgttcgcggt cccaccagag cgccccgatc acgcgggcag tgagcgcctc
     2641 gctctctcct gcccggacgt aggcggagta gttgtccaac cagaaccggt cggacacgat
     2701 cgcgtcctgc cacgcggtga tcccgaggga gtggaggtag cgctgcccct ccaagaggcc
     2761 ttcgtagacg tcgtccggcg tggcggcc
//
LOCUS       DASUVE010000096         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_234469, whole genome shotgun sequence.
ACCESSION   DASUVE010000096 DASUVE010000000
VERSION     DASUVE010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234469"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /gene="hisS"
                     /locus_tag="VF036_01950"
     CDS             complement(<1..1066)
                     /gene="hisS"
                     /locus_tag="VF036_01950"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX6264023.1"
                     /translation="MDLRPARGTLDLLPPQGSRMRALYDRAAALARLHGYRYVETPGF
                     EATELFSATSGETSDVVTKEMYTFDDRKGRSLTLRPEGTAPVMRAYLDAMQEQPSPFK
                     AYYLTRMYRYARPQEGRYREHRQFGIEVFGTEAPGADAEVIAMGDAFLRELGLNRIEL
                     QLNSIGDEVCRPAYRDELVAFLRANRDRLHDEHRDRFEDNPLRVLDCKDEACRAVARE
                     APRITDRLCEPCLAHFEGVQSGLKDAGLSWTLEPTLVRGLDYYTRTAFEFVSPGLSPQ
                     QATLFGGGRYDGLAEVLGGPHVPGVGFGMGLERVLLALEQEGIEAPEEPGLAVFVVGV
                     GDAGHARAHELVRELRAGGVA"
     gene            complement(1066..1686)
                     /locus_tag="VF036_01955"
     CDS             complement(1066..1686)
                     /locus_tag="VF036_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6264024.1"
                     /translation="MYQDVFDRNPFGTNCWLLGADGGEDAVLVDPGFEPDELRAVLER
                     AGRRPTAIRLTHAHLDHASVAGVLAGDDLPVFVHEADAVAFEDIDAWNPGFDNPLDPV
                     KDLRRVVDGDELSFAGISIGVRHTPGHTPGHCCFVTDATVISGDLVFAGSIGRSDFPN
                     SSPEAMQASLRWFLSRDDADAVLPGHGPRTTVGRERSSNPFLVHVA"
     gene            1759..2163
                     /locus_tag="VF036_01960"
     CDS             1759..2163
                     /locus_tag="VF036_01960"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEX6264025.1"
                     /translation="MERHIVFYDEDCGFCRWSVDRLLRWDRRGLLRASPIQGEEADRL
                     LTDLREEDRLASWHLVAPDGGRYSGGAAAGPLARLLPAGAPVALLAETFPRTTERLYR
                     WVARNRDSFGRRLGVEACAVDPSGRSRRTTPS"
     gene            complement(2150..2605)
                     /gene="dtd"
                     /locus_tag="VF036_01965"
     CDS             complement(2150..2605)
                     /gene="dtd"
                     /locus_tag="VF036_01965"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006723124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX6264026.1"
                     /translation="MRIVLQRVAHASVTVHGDRVAEIGRGYLLLVGVGRGDPGDDAEK
                     LADKIVHLRLFADDQGKTNRSLADVDGEVLVVSQFTLYADLRKGRRPSWGDAEDPTIA
                     AERVEALAAALEARDVRVGRGVFGATMDVELLNDGPFTLVLEGASLSSA"
     gene            complement(2672..>2907)
                     /locus_tag="VF036_01970"
     CDS             complement(2672..>2907)
                     /locus_tag="VF036_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012087237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6264027.1"
                     /translation="VAIQVEALDRTRLLSDVATVLSDSHVNILAATSSTGRDRITRLR
                     FTFEMADVTHLSSLLSAVKRVESVYDAYRVVPN"
BASE COUNT          432 a         1054 c          998 g          423 t
ORIGIN      
        1 cggcgacgcc gcccgcccta agctcccgca ccagttcgtg cgcccgtgcg tggccggcgt
       61 cgccgacgcc gaccacgaac accgcgagtc ccggctcctc cggcgcttcg atcccctcct
      121 gctcgagcgc gaggaggaca cgttccaggc ccatcccgaa cccgacgccg gggacgtgcg
      181 gcccgcccaa cacctcggcc agcccgtcgt accggccacc gccgaacagc gtggcctgct
      241 gcggcgagag acccgggctc acgaactcga acgccgtccg cgtgtagtag tcgaggccgc
      301 ggaccagcgt cggctcgagc gtccacgaga ggccggcgtc cttcagcccg gactgcacac
      361 cttcgaagtg agccaggcac ggctcgcaga gccggtcggt gatgcgcggg gcttccctcg
      421 caaccgcgcg acacgcttcg tccttgcagt cgaggacgcg gagcgggttg tcctcgaaac
      481 ggtcccgatg ctcgtcgtgc agccggtccc ggttggcgcg caggaacgcc acgagctcgt
      541 cgcggtacgc gggacggcag acctcgtccc cgatcgagtt gagctgcagc tcgatccggt
      601 tcagcccgag ctcgcgcagg aacgcgtcgc ccatcgcgat cacctcggcg tcggcgccgg
      661 gcgcctcggt cccgaacacc tcgatgccga actgccggtg ctcgcggtag cgaccttcct
      721 gcggccgggc gtaccggtac atcctcgtga ggtagtacgc cttgaacgga gacggctgct
      781 cctgcatcgc gtcgaggtag gcgcgcatca ccggcgcggt tccctctgga cgcagcgtca
      841 gcgaccgccc tttccggtcg tcgaacgtgt acatctcctt cgtgacgacg tcactcgtct
      901 cgcccgacgt ggcggagaag agctcggtgg cctcgaagcc gggcgtctcc acgtagcggt
      961 acccgtggag ccgggcgagg gccgccgcgc gatcgtagag cgcccgcatg cggctgccct
     1021 gcggagggag caggtcgagc gtgcctcgtg ccggccgcag gtccatcagg ccacgtgcac
     1081 caggaacggg ttggacgagc gttcccgtcc cacggtggtc cgcggaccat gccccggaag
     1141 gacggcgtcg gcgtcgtccc tcgacaggaa ccatcggagg ctcgcctgca tcgcctcggg
     1201 cgaggagttc gggaagtcgg agcggccgat cgagccggcg aacacgaggt ccccggagat
     1261 caccgtggcg tcggtcacga agcagcagtg accgggtgtg tgccctgggg tgtggcgaac
     1321 gccgatcgat atcccggcga acgacagctc gtcaccgtcg acgacgcggc gcagatcctt
     1381 cacgggatcc agggggttgt cgaacccggg gttccaggca tcgatgtcct cgaacgcgac
     1441 ggcgtcggcc tcgtggacga acacggggag gtcgtcgccg gcgagcaccc ccgcgacgga
     1501 ggcgtgatcg agatgtgcgt gcgtcaggcg gatcgccgtc ggtcgccgcc cggcgcgctc
     1561 caggaccgct ctgagttcgt cgggctcgaa accggggtcg acgagcaccg catcctcgcc
     1621 gccatcggcg ccgaggagcc aacagttcgt gccgaacggg ttccgatcga agacgtcctg
     1681 gtacatctgg cgcatgctac cgggcccggc gtggccgccc cgaccggctc cgcacggtgg
     1741 ctgcgtacgc tggcacccat ggagcgacac atcgtgttct atgacgagga ctgcggcttc
     1801 tgccgatggt cggtcgaccg gctgttgcgg tgggatcggc gcggtctcct ccgggcctcg
     1861 ccgatccagg gcgaggaggc cgatcgcctc ctcaccgacc tgagggagga ggaccggctc
     1921 gcctcctggc acctcgtcgc gcccgacgga ggtcgctact ccggcggcgc ggcggccggc
     1981 ccgctggctc gtctcctgcc ggccggcgcc cccgtcgctc tcctggcgga gacgttccct
     2041 cgcaccaccg aacgcctcta ccggtgggtg gcgcggaacc gggactcgtt cggccgacgg
     2101 ctcggggtag aggcgtgcgc cgtcgatccg agcgggcgat ctcggcgaac tacgccgagc
     2161 tgagcgacgc gccttccagc accaacgtga acggaccgtc gttgaggagc tccacgtcca
     2221 tcgtcgcgcc gaacacaccg cgtccgacac gcacgtcacg agcttccaac gccgcagcga
     2281 gcgcttcgac ccgctccgcg gcgatcgtcg gatcctccgc gtccccccag gacggacgac
     2341 gccccttccg gaggtccgcg tagagggtga actgggacac cacgaggacc tcgccgtcga
     2401 cgtccgccag ggaacggttc gtcttcccct gatcgtcggc gaagagtcgg aggtgcacga
     2461 tcttgtccgc gagcttctcc gcgtcgtccc cgggatcccc gcgcccgacc ccgacgagca
     2521 gcaggtagcc gcgtccgatc tccgccacgc gatccccgtg caccgtcacg gaggcgtgcg
     2581 ccacgcgctg caggacgatc ctcatatcgg acccggtcga cctcccctcc ggtcggcgtc
     2641 gcaggacccg gaccggtccc tggagaccac ctcagttcgg gacgacgcgg taagcgtcgt
     2701 agacactctc cacccgcttc accgcggaca gcagcgacga gaggtgcgtc acgtcggcca
     2761 tctcgaacgt gaaccggagg cgcgtgatcc ggtcccgacc cgtgctggag gtggcggcga
     2821 ggatgttgac gtggctgtcg gacaggacgg tggccacgtc ggagagcagc cgcgtccggt
     2881 cgagggcctc cacctggatc gccaccg
//
LOCUS       DASUVE010000097         4050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1176526, whole genome shotgun sequence.
ACCESSION   DASUVE010000097 DASUVE010000000
VERSION     DASUVE010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176526"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /gene="thiL"
                     /locus_tag="VF036_01975"
     CDS             <1..742
                     /gene="thiL"
                     /locus_tag="VF036_01975"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX6264028.1"
                     /translation="MAGSPRFALCALTLTDAIDASWVVELAGGMRECCDEFAVSLVGG
                     NLSRGEELAIVVTVTGEVGPGRAVRRDGARPGERVVVTGSLGGSAAGLRVSSQRTWSD
                     DERDALRRSMRPVPRVGEASILAAHGATAMMDVSDGLAIDLSRLCRASSVAARVEVGH
                     VPVHPASTLREALGGGEDYELVATLPGPDAVAGAAKDVHEGFGVSLNDIGVIIEREGE
                     AESAGGLVAVEEDGTERPLAIEGWDHFR"
     gene            739..1587
                     /gene="thiD"
                     /locus_tag="VF036_01980"
     CDS             739..1587
                     /gene="thiD"
                     /locus_tag="VF036_01980"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX6264029.1"
                     /translation="MTDGPSRRQGPLRARALTIAGSDSGGGAGIQADLKTFSALGVFG
                     MTAITAVTIQNTKGVAGFEELSPTTVAEQIRAVAGDIGVDAAKTGMLASAAIVEAVAE
                     SAAEMSLPNIVVDPVFVSKHGHPLLADDAVDALRRSILPLATLVTPNLAEAAGLAGLD
                     VVSRDDMRRAATTILALGPRAVLVKGGHLEGDRATDLLVDGEREEWLEADRIQTPNTH
                     GTGCTLSAAIAAHLARGAPLADAVREGKAFVTEAIRHALPLGDGIGPVDQLWSIGVAQ
                     KGPEAR"
     gene            complement(1591..1794)
                     /gene="rpmB"
                     /locus_tag="VF036_01985"
     CDS             complement(1591..1794)
                     /gene="rpmB"
                     /locus_tag="VF036_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003785965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX6264030.1"
                     /translation="MAAVCDICGKKPWFGKSVTFSHKRNSRRWDPNIQRVRALVDGTP
                     GRINACTSCIKAGRVTRIPQRQP"
     gene            complement(1820..2362)
                     /locus_tag="VF036_01990"
     CDS             complement(1820..2362)
                     /locus_tag="VF036_01990"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6264031.1"
                     /translation="MTSRRLIAAAAGLLLLVACDREPVGPPAQGEIPAHNATETPLLP
                     TFADTLPTMDADTFERLLSQLEGTPVLVNFWGSWCPPCKDEMPRLVAAHDEFGDRVQF
                     LGVDIMDNREDAKVFMEEYGMTFPSVFDVPDVIKTSLGQFGQPTTVFFRADGSFEFAY
                     AGPIEENILAKHLERIAGTG"
     gene            2369..>4050
                     /locus_tag="VF036_01995"
     CDS             2369..>4050
                     /locus_tag="VF036_01995"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6264032.1"
                     /translation="MVEPFPNVPGSSSALALDSPVLHVDRKVATRRTGLRNKGEEAHV
                     ILAGMDVRTVGDLLRHYPRRYIDRSAVERIRDLQVGEQATVIARVHATVKRLTRRRQS
                     MVTVTISDGTGKLDLTFFNQPWAAGIYKAGLELAVSGTVTRYRGRLQLANQEAEILGG
                     DERELVHTGRITPVHRASDGIATRTIRELVFSALERLPSIPDPIPSGVIETEGLEDLD
                     TALRRVHFPEDAEQLARAIERLKFDELFTLELGVALRKHRLESEETGIAHQADGELTD
                     RLVATLPFEPTKAQTRAIEEVGAAMAAARPMNVLLQGDVGSGKTLVAVSACLAAIQSG
                     HQAAIMAPTEVLATQHARSVAALLDGVGAIDVLERGSSGTPAPDGQGSLLEALAVTPP
                     EGDPVTYALLTAAVTGKDRARILAGVADGGVDLVIGTHALVQEAVSFADLSLAVVDEQ
                     HRFGLHQRMALKGKGDGDIDVVIMTATPIPRTLALTYYGDLDVVVLDEMPKGRQPIGT
                     AAARSDEERAAAYDLVRREVQEGRQAFVVCAAIDEGNRSQVRAAEAEAERLAT"
BASE COUNT          609 a         1357 c         1480 g          604 t
ORIGIN      
        1 catggcgggg agcccgcgat tcgcgctctg cgcgctgacc ctcaccgacg cgatcgacgc
       61 ctcgtgggtc gtcgagctgg ccggggggat gcgggagtgc tgcgacgagt tcgcggtgtc
      121 gctcgttggc gggaacctct cgcgcggtga ggagctcgcg atcgtcgtca ccgtcaccgg
      181 cgaggtcgga ccggggcgcg ccgtccgccg ggacggggcc cgtcccggcg agcgggtggt
      241 cgtgacgggg tccctcggcg gctcggccgc cggtctgcgg gtctcctcgc aacgcacctg
      301 gtccgacgac gagcgcgacg cgctgcgtcg ctcgatgcgc cccgtcccgc gcgtcggcga
      361 ggcctcgatc ctggctgccc acggggccac cgcgatgatg gacgtatccg acggcctcgc
      421 gatcgatctt tcccggctct gccgggcgag ctcggtcgcg gcacgggtcg aggtcgggca
      481 cgtgccggtg cacccggcgt cgacgctgcg cgaggcgctc ggtgggggag aggactacga
      541 gctggtcgcg accctccccg gccccgatgc ggtcgcagga gcggcgaagg acgtccacga
      601 ggggttcggc gtgtcgctca acgatatcgg cgtcatcatc gaacgcgagg gcgaggccga
      661 gagcgcggga ggactcgtgg ccgtggagga agacggaacg gagcgccccc tcgcgatcga
      721 agggtgggat cacttccgat gaccgatgga ccgtcccggc gacagggacc tcttcgcgcg
      781 cgagcgctga cgatcgccgg atccgactcc ggcggtggcg ccgggatcca agccgacctg
      841 aagacgttca gtgcgctcgg cgtgttcggc atgacagcga tcacggcggt caccatccag
      901 aacacgaagg gcgtcgccgg attcgaggag ctctcaccca cgacggtggc cgagcagatc
      961 cgcgcggtgg ccggcgacat cggtgtcgat gcggccaaga cggggatgct cgcgtcggcc
     1021 gccatcgtcg aggccgtggc ggagtcggcc gcggagatgt cgctcccgaa catcgtcgtc
     1081 gaccccgtct tcgtctcgaa gcatgggcat ccgttgctgg ccgacgacgc cgtcgacgca
     1141 ctccggcgat cgatcctgcc tctcgcgacg ctcgtgacgc cgaaccttgc agaggcggcc
     1201 gggttggccg ggctcgacgt ggtgagccgc gacgacatgc ggcgagctgc cacgacgatc
     1261 ctcgcgctcg gaccccgcgc cgtcctcgtg aagggagggc acctcgaggg cgatcgcgcg
     1321 accgacctgc tcgtcgacgg agagcgggag gaatggctcg aggccgatcg gatccagacg
     1381 ccgaataccc acggtaccgg ctgcacgttg tccgctgcga tcgccgcgca tctggctcga
     1441 ggggcgccgc tcgccgacgc ggttcgggag gggaaggcgt tcgtcacgga ggcgatccgg
     1501 cacgccctcc cgttgggtga cgggatcggt ccggtcgacc agctgtggtc gatcggggtc
     1561 gcgcagaagg gtccggaagc gcgctgagac ctagggctgc cgttgcggga tgcgggtgac
     1621 ccggccggcc ttgatgcagc tcgtgcacgc gttgatgcgg cccggcgtgc cgtcgacgag
     1681 cgcgcggacg cgctggatgt tggggtccca ccgtcggctg ttccgcttgt gcgagaacgt
     1741 caccgacttg ccgaaccacg gcttcttgcc acagatgtca cagaccgctg ccatagatgc
     1801 gtcgaggcta ccagagcgtt catccggtcc ccgcgatgcg ttcgaggtgc ttggccagga
     1861 tgttctcctc gatcgggccc gcgtaggcga actcgaagga cccgtcggct cggaagaaga
     1921 ccgttgtggg ctggccgaat tggccgagcg aggtcttgat cacgtccggg acgtcgaaga
     1981 cgctcgggaa cgtcatcccg tactcctcca tgaagacctt cgcgtcctcc cggttgtcca
     2041 tgatgtcgac accgaggaac tgcacccggt cgccgaactc gtcgtgcgcg gcgacgaggc
     2101 ggggcatctc gtccttgcag ggcgggcacc acgatcccca gaagttgacg aggaccggtg
     2161 tcccttccag ctgtgagagc agccgctcga acgtgtccgc gtccatcgtc ggcagggtgt
     2221 cggcgaacgt tggcaggagc ggggtctcgg tggcgttgtg ggcagggatc tccccctggg
     2281 ccgggggacc gaccggctcg cgatcgcacg cgacgaggag caggagcccg gccgcggcgg
     2341 cgatgaggcg tcgagacgtc acacgtggat ggtagaaccg ttcccgaacg tgcccggttc
     2401 ctcctccgct ctcgcgctcg attccccggt cctgcacgtg gaccggaagg tcgcgacccg
     2461 ccgcaccggc ctgcggaaca agggggagga ggcgcacgtg atcctggccg ggatggacgt
     2521 ccgaaccgtc ggcgatctgc tccggcacta cccgaggcgg tacatcgacc gctccgccgt
     2581 ggaacggatc cgcgatctgc aggtcggaga gcaggcgacc gtgatcgctc gagtgcacgc
     2641 cacggtgaag cgactcacgc gtcgccggca gtcgatggtg accgtcacga tctcggacgg
     2701 caccggcaag ctggacctca cgttcttcaa ccaaccctgg gcggcgggca tctacaaggc
     2761 gggtctggag ctcgccgtct cgggcaccgt gacccggtac cggggccgcc tccagttggc
     2821 caaccaggag gcggagatcc tcggcggcga cgaacgggag ctcgtgcaca cgggacggat
     2881 caccccggtc caccgtgctt ccgacgggat cgccacccgc acgatccggg agctggtgtt
     2941 ctccgcgctc gagcggctcc cgtcgatccc ggacccgatc ccctccggcg tgatcgagac
     3001 cgaagggctc gaggatctcg acacggcgct gcggcgggtg cacttcccgg aggacgcgga
     3061 gcagctcgcg cgggcgatcg aacggctcaa gttcgacgag ctgttcacgc tggagctcgg
     3121 ggtcgcgctc cgcaagcatc ggctcgagtc ggaggaaacc gggatcgcgc atcaggcgga
     3181 tggagagctg accgaccggc tcgtcgcgac cctgcccttc gagcccacga aggcccagac
     3241 ccgtgcgatc gaggaagtcg gggcggcgat ggccgcggct cgcccgatga acgtcctcct
     3301 gcagggcgac gtcggttccg ggaagacgct cgtggccgtg tcggcatgcc tcgcggcgat
     3361 ccagtcgggc catcaggccg ccatcatggc gcccaccgag gtcctcgcca cccagcacgc
     3421 ccgctccgtc gcggccctgc tcgacggggt gggtgcgatc gatgtcctcg aacgcgggtc
     3481 gagtggcacg cccgcgcccg acggccaggg ctccctgctc gaagcactcg cggtgacgcc
     3541 accggaaggc gatccggtca cgtacgcgtt gctcaccgca gccgtcaccg ggaaggatcg
     3601 ggcccggatc ctcgccggtg tcgcggacgg cggtgtggat ctcgtgatcg ggacccacgc
     3661 cctcgtgcag gaagcggtgt ccttcgctga tctctccctg gcggtcgtgg acgagcagca
     3721 ccggttcggt ctccatcaac ggatggcgct caagggcaag ggcgacggcg acatcgacgt
     3781 ggtgatcatg accgcgacgc ccatcccgag gacgctggcg ctcacgtact acggcgacct
     3841 cgacgtggtg gtgctcgacg agatgccgaa gggtcgccag ccgatcggga cggcggcggc
     3901 tcgctcggac gaggaacgcg ccgccgccta cgacctggtg cggcgcgagg ttcaggaggg
     3961 acgacaggcc ttcgtcgtgt gcgccgcgat cgacgagggg aatcgttcgc aggtgcgagc
     4021 ggccgaagcc gaagccgagc ggctcgccac
//
LOCUS       DASUVE010000098         3698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_235696, whole genome shotgun sequence.
ACCESSION   DASUVE010000098 DASUVE010000000
VERSION     DASUVE010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235696"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VF036_02000"
     CDS             complement(<1..757)
                     /locus_tag="VF036_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264033.1"
                     /translation="MRRFAVAIVVLLVACTSEPPPPSPSETPSETPSGDPVEVVTGPT
                     SAAEAMRRLCVAPDVEVDPVKVVPTPPAIAEVADEVESVRGLEFDRPVNVEPITEAEM
                     DRRLADYLATYYPRRFYARRSEAWQTIGALPRDVGYLEAIDAYQQGQVLGFYNSQNEE
                     LVYTGDAELNRIEQFVLAHELTHAIDDQHFDLDRLDGLVARCQEERFQAALGIVEGSA
                     NHFATQVSIRFPIQDVGSIPGDDAGEVPQFIVEI"
     gene            complement(754..924)
                     /locus_tag="VF036_02005"
     CDS             complement(754..924)
                     /locus_tag="VF036_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264034.1"
                     /translation="MIAYLAAVVLWVAAIVTLVVSTAGFLESTMLLTASAVLSGLAIV
                     AAVSALVVSRRR"
     gene            complement(921..2225)
                     /locus_tag="VF036_02010"
     CDS             complement(921..2225)
                     /locus_tag="VF036_02010"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX6264035.1"
                     /translation="MEAVTSLAKRRGIAFQSSEIYGGLRSSWDYGPLGTEIKRNVREQ
                     WWRSTVQLRDDVVGLDASVIMSPKVWEASGHLEVFTDPLVECLNCHQRFRADELPEPA
                     ACPNCGQTAFTQPRNFHLMFKTNMGPIEDAENTVYLRPETAQGMFVDFATIQQVSRKK
                     IPFGIAQIGRSFRNEITPGNFIFRQREFEQMEMEFFVEPGTDEDWHEFWIGERMRWWT
                     DLGLREDHVRIREHDADELSHYSKRTVDIEYRFPFTDWGELEGIANRTDFDLRAHQAA
                     SGEDLTYFDQEKNERYHPYVIEPAAGVDRAVLAFLLDAYREEEAPAAKGGTEKRSYLA
                     LDRRLAPTKVAVLPLSRNEKLAPEARRVFDLVKSEWMCDFDDAGSIGRRYRRQDEVGT
                     PFCVTVDFDSLDDAQVTVRERDSMGQDRLPIDGLVDHLTERL"
     gene            complement(2905..>3698)
                     /locus_tag="VF036_02015"
     CDS             complement(2905..>3698)
                     /locus_tag="VF036_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264036.1"
                     /translation="DDRLHAVHEVYRLASERVGRLQQKLEESEEARRRLEGRMQQALS
                     KASPSAPVVSPPAPVAEAQPEPAVVQVPESVVLKKSRSDDAWAASPAGPLARAARDQK
                     PPPATSSAPRQPVDKQKRVRKPKPEKTEKPEKPEKPAKQQPPKRAPAKTEPTGASDPI
                     VVPEPTPAPERRVAALATTPASPPAAPTAADPELADAEAHEAALEMFIRLTESDRQAH
                     ETTEVDQGAVRAALARTAARKKPGGERLQPHEGPPGESPGGPPRS"
BASE COUNT          516 a         1271 c         1274 g          637 t
ORIGIN      
        1 tgatctcgac gatgaactgc gggacctcgc cggcgtcatc accggggatc gagccgacgt
       61 cctggatggg gaaccggatc gacacctggg tcgcgaagtg gttcgcgctc ccctccacga
      121 tgccgagggc ggcctggaag cgttcctctt gacagcgcgc cacgagaccg tcgaggcggt
      181 cgaggtcgaa atgctgatcg tcgatcgcgt gcgtgagctc gtgggccagc acgaactgct
      241 cgatccggtt gagctcggcg tcgccggtgt agacgagctc ctcgttctgc gagttgtaga
      301 agccgaggac ctgtccctgc tgataggcgt cgatggcctc caggtatccg acgtcgcggg
      361 gcaacgcgcc gatcgtctgc cacgcctcgc tcctgcgagc gtagaaccgc ctcgggtaat
      421 aggtggcgag gtagtcggcc aagcgccggt ccatctcggc ttccgtgatc ggctcgacgt
      481 tcaccggccg gtcgaactcc agcccgcgca ccgactccac ctcatcggca acctccgcga
      541 tcgccggcgg cgtgggcacc accttcacgg gatccacctc gacgtcgggg gcgacgcaga
      601 ggcggcgcat cgcctcggcc gccgaggtcg gccccgtcac gacctcgacc gggtcgccgg
      661 acggcgtctc cgacggcgtt tccgacggtg acggtggtgg cggctccgac gtgcaggcca
      721 cgaggagcac cacgatcgcg acggcgaacc gcctcaccgg cgcctcgaca ccacgagcgc
      781 gctcaccgcg gccacgatcg ccagtcccga gagcacggca gacgccgtga ggagcatcgt
      841 ggactcgagg aaccccgcgg tggagacgac cagcgtcacg atcgccgcta cccagaggac
      901 gaccgccgcg aggtaggcga tcacaggcgc tccgtcagat ggtcgacgag gccgtcgatc
      961 ggcaggcggt cctgccccat cgaatcacgt tcccggaccg tgacctgtgc gtcgtccagg
     1021 gagtcgaagt cgaccgtgac gcagaacggc gtgccgacct cgtcctgccg ccggtagcgt
     1081 cgcccgatcg accccgcgtc gtcgaaatcg cacatccact cgctcttcac caggtcgaag
     1141 acgcgccggg cttcgggggc gagcttctcg ttgcgtgaca gtgggagcac cgcaaccttc
     1201 gtcggcgcca gccgacggtc gagcgcgagg tagctgcgct tctcggtccc gcccttcgcg
     1261 gccggcgcct cttcctcccg gtaggcgtca aggaggaacg cgaggaccgc gcgatcgacg
     1321 cccgcagcgg gctcgatcac gtacgggtgg taccgctcgt tcttctcctg gtcgaagtag
     1381 gtgaggtcct cgccggaggc cgcctggtgc gccctgaggt cgaagtcggt acggttcgcg
     1441 atcccctcga gctcccccca atcggtgaac gggaaccgat attcgatgtc gaccgttcgc
     1501 ttcgagtagt gcgacagctc gtcggcgtcg tgctcgcgga tccggacgtg gtcctcacgg
     1561 agcccgaggt cggtccacca gcgcatccgc tcaccgatcc agaactcatg ccagtcctcg
     1621 tccgtccctg gctcgacgaa gaactccatc tccatctgct cgaactcccg ctggcggaag
     1681 atgaagttcc ccggcgtgat ctcgttccgg aacgatctgc cgatctgcgc gatcccgaaa
     1741 gggatcttct tgcggctcac ctgttggatc gtggcgaagt cgacgaacat cccctgtgca
     1801 gtctcgggac gcagatacac ggtgttctcc gcgtcctcga tcgggcccat gttggtcttg
     1861 aacatcaggt ggaagttgcg gggttgcgtg aaggctgtct gaccgcagtt cgggcacgcg
     1921 gccggctcgg ggagctcgtc ggcccggaat cgctggtggc agttcaggca ctcgaccaat
     1981 gggtccgtga agacctcgag gtgaccggac gcctcccaga ccttgggcga catgatcacg
     2041 gaagcgtcga ggccgaccac gtcgtcgcgg agctgcacgg tcgaacgcca ccactgttcg
     2101 cggacgttgc gcttgatctc ggtgccgagc ggcccgtaat cccaggacga gcggagcccg
     2161 ccgtagatct ccgaggactg gaacgcgatc ccccgccgtt tggcgaggga ggtgacggcc
     2221 tccatcaggt cggccattga tcttcgactc cttcccggtc gctggctgcg gccgccggcg
     2281 tgccgctcat gctaccgggg tcaatcgagg ttgccgccgt tccgatacct cagggatgcc
     2341 cgtcggatca ctctgcaggt catgtggcac cgcccttcct ccggacatcc ggtggtgcac
     2401 gacctgctac acgcggtgac gctgtacgcg ccccgcgatc cgctgcacga gccgggtggc
     2461 ttcgtcggtc tcccgacgca cacgccgagg acctctcgat ggcggtcggg accgacgtcg
     2521 ttcggcccgg tgggccggat cgtgtgcacc ctcgcaatcg cggcgctgtt cccctggtgg
     2581 ggactcggcg gtttcaaccc gttcttcctt tgggcgctga tgggatgggt gcttgctgcg
     2641 gtgttcatgc tcaggagcat ctggaaaccg gaacgcatcg tggatccgac gccgaccagg
     2701 atcgagcggc tccgtgcccg ccacccagtg ctggggatgg agctccgcct cagcggaggg
     2761 gtaggcctgg tcgtgctgat cctcggtggg tgtggcggtc accgcctggg tctccctgga
     2821 cgatgtgtcc cgctacgtct gggccatcgc ggcgatcgtc gcgggcgtcg gcatcttcct
     2881 cgcgagctgg aacgatctgt agacctacga ccggggcggg ccgccgggcg attcgccggg
     2941 cggaccttcg tgcggctgca ggcgctcgcc gcccggcttc ttgcgggccg cggtccgggc
     3001 aagggccgcg cggacagcgc cctggtccac ctcggtggtt tcgtgagcct gccggtcgct
     3061 ctcggtgagg cggatgaaca tctcgagggc ggcctcgtgg gcctcggcgt ccgcgagctc
     3121 gggatcggcc gcggtcggcg cggccggcgg gctcgccggg gtggtcgcca acgcggccac
     3181 acggcgctcg ggcgccgggg tgggctccgg caccacgatc gggtccgagg cgccggtcgg
     3241 ctccgtcttg gccggcgccc gcttcggcgg ctgctgcttc gccggcttct ccggcttctc
     3301 cggcttctcc gtcttctccg gcttcggctt ccgcacacgc ttctgtttgt cgaccggctg
     3361 cctcggggcg ctcgacgtcg ccggcggagg cttctgatcc cgggccgcgc gggcgagcgg
     3421 accggcgggc gaagcggccc aggcatcatc cgaccgcgac ttcttcagaa caacggactc
     3481 cgggacctgg acgacggccg gttcgggctg ggcctcggcc accggtgccg gtggcgagac
     3541 gacgggcgcc gaaggggatg ccttcgacag ggcctgctgc atgcggccct ccaggcgccg
     3601 ccgcgcctct tcggactcct cgagcttctg ctgcagccgg ccgacgcgtt ccgaggcgag
     3661 acgatagacc tcgtggacgg cgtgcagccg gtcgtcgt
//
LOCUS       DASUVE010000099         4562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_425814, whole genome shotgun sequence.
ACCESSION   DASUVE010000099 DASUVE010000000
VERSION     DASUVE010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425814"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VF036_02020"
     CDS             complement(<1..423)
                     /locus_tag="VF036_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264037.1"
                     /translation="MSLADHTSRVPVFARPKSDAPRRRATDSVVGGPIGAQPSRRVGD
                     GWLDRWLETVADLARLKDSHPMLDAVIDEQDGRRIRIGDRWLFDFASCNYLGFDLDRE
                     IIDAIPAYLDAWGTHPSWSRLLGSPVLYEEIEDRLTNLL"
     gene            complement(593..1561)
                     /locus_tag="VF036_02025"
     CDS             complement(593..1561)
                     /locus_tag="VF036_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6264038.1"
                     /translation="MARARIRCVWVVLVVLCLTVCAQVEDPGSRPTSPVRSPRLPDGS
                     ITVAAAGDISCPSDDSDFEGEDPSECQQHATAALLVDADAVLVLGDLQYPDGTLGTFE
                     AGYDVSWGRYADKTYPAVGNHEYHVDDAAGYFDYWAAKGRPTGEEGSGYYSFDLGSWH
                     LVALNSECDPVLCEEGSPQDSFLEGDLMGTSQPCILAFWHHPLFNSGATHGDSAPSGA
                     RAFWDDLTVAGADIVLNGHEHNYQRYGKQTGAGRAASEGIRQFVVGTGGKALYPMLVE
                     KDPNFEAGQASEFGVLKLHLGEGAYSWEFVAIGGTSLDSGGPVPCN"
     gene            1652..2614
                     /locus_tag="VF036_02030"
     CDS             1652..2614
                     /locus_tag="VF036_02030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015102202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03885 family FMN-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6264039.1"
                     /translation="MPTLGIHASHEQLSPHDLLEVVRAAEAAGFPAAMCSDHLAPWSE
                     RQGESGHAWAWLGAAMQATSLPFGVVTAPGQRYHPAVAAQAIATLGDLFPGRFWAALG
                     SGEALNEHVTGDPWPQKEDRDVRLLECVDVIRALLRGDEVTHDGLVRVDRARVWSLPA
                     EPPALYGAAVSEETARTVASWADGMITVYQPIDVLRRVVDAFREGGGDRAPVAVQVHV
                     SWAEDEETALAIAHDQWRTNVFGSELAWNLELPAQFDEAAARVRPDDVSEAVLVSADL
                     GQHAKWLLELADLGVDAIYVHHVGKELDAFIEAFAEHVLPELAS"
     gene            2611..4284
                     /locus_tag="VF036_02035"
     CDS             2611..4284
                     /locus_tag="VF036_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEX6264040.1"
                     /translation="MTTARTSDLWWKSAIVYCLDVETFLDWDGDGTGDLIGLTERIDY
                     LAGIGISCLWLMPFQPSPNRDDGYDITDYYGVDERLGSFGDFVALIRTAKDRGMRVII
                     DLVVNHTSDRHPWFRAARSSRTSPFRDWYVWSDDIPEEPKAEVVFPDAEDSIWAWDEK
                     AGQYYLHHFYSHQPDLNIANPKVREELARIVGFWLELGVDGFRVDAVPFLLETGGIAG
                     DVEIDPHDFLRDLRSFMARRRGDVMMLGEVNLPHKDLQLFFGGPDGDEVQLLFNFILM
                     QSIYLSLARQDARPLAKALRETVRKTDDQQYANFLRNHDELTLDKLTDAQRQEVFDAF
                     GPEEDMQLFGRGLRRRLPSMLDGDEQRIRMAYSLLFSLPGTPVLFYGEEIGMGENLAI
                     EGRLSVRSPMQWTDEKNGGFSSAPPSRLRRPVVEGRFGPMAVNAAAQRRDPDSLLTWM
                     ERLIRRRRETPELAWGAWHVLETDVVGVFAHRCDWEARSVVAVHNLGADPCIVRVELG
                     DVPEGAKLDDLLDERGALDEIDGAALELKLGGYGYRWFRIGTPDQRTPP"
     gene            complement(4287..>4562)
                     /locus_tag="VF036_02040"
     CDS             complement(4287..>4562)
                     /locus_tag="VF036_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019420072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6264041.1"
                     /translation="GIRDEDLPRIFDRFSRVGIDRSRSDGGSGLGLSITKAIVDAHGG
                     SIDVFSEEGCGATFRIRLGGFLEDQTQTQVRVPLPMDRVMSETGIVD"
BASE COUNT          720 a         1569 c         1570 g          703 t
ORIGIN      
        1 gagcaggttc gtaaggcgat cctcgatctc ctcgtagaga accgggctgc cgagcaggcg
       61 cgaccagctc ggatgggtgc cccatgcatc gagatacgcg gggatcgcgt cgatgatctc
      121 gcggtcgagg tcgaagccga ggtagttgca cgacgcgaag tcgaagagcc atcggtcacc
      181 gatccggatc cgccggccgt cctgctcgtc gatcacggcg tcgagcatcg gatgggagtc
      241 cttcaaccgg gcgaggtccg cgacggtctc cagccatcgg tcgagccacc cgtcgcccac
      301 gcgccgggag ggctgcgctc cgatcggacc gcccaccacc gagtcggtcg cccgccggcg
      361 gggggcatcg gacttcgggc gagcgaacac gggtacgcgc gaggtatggt cggcgagcga
      421 catggaatcc ccatcggccg aatgggcgag gggctttaca cctacaacgg ggtatctcgt
      481 gcccgggtga gcacgctggc acgcggctgg aggcccccga aggggggaca cgagctccca
      541 gctccgttcc ccgatcgggg gacagcacga gcgagctggt acggaggcgg gctcaattgc
      601 acgggaccgg accgcccgaa tcgagcgagg tcccgccgat ggcgacgaac tcccatgagt
      661 acgcgccctc tccgaggtgg agcttcagca caccgaactc gctcgcctgc ccggcctcga
      721 agttgggatc cttctccacc agcatcggat agagggcctt cccaccggtg ccgacgacga
      781 actggcggat cccctccgat gctgcccgcc cggctccggt ctgcttcccg taccgctggt
      841 agttgtgctc gtgaccgttc aggacgatat cggcccccgc aaccgtcagg tcgtcccaga
      901 acgcgcgcgc tccggacggc gccgagtcgc cgtgtgtcgc ccccgagttg aacagcggat
      961 ggtgccagaa ggcgaggatg cacggctgcg acgtgcccat caggtcgccc tcgaggaagc
     1021 tgtcctgcgg tgagccctcc tcgcagagga cgggatcgca ttcggagttc agggccacga
     1081 ggtgccacga gccgaggtcg aagctgtagt agcccgaacc ctcttctccc gtgggccggc
     1141 ccttcgcggc ccagtagtcg aagtacccgg ccgcatcgtc gacgtggtac tcgtgattgc
     1201 cgacggccgg gtacgtcttg tccgcgtacc gcccccagct cacgtcgtag ccggcctcga
     1261 acgttcccaa ggtcccatcc ggatactgga ggtcgccgag gaccaggacg gcatcggcgt
     1321 caacgagcaa ggccgcggtc gcgtgctgtt gacactcgct ggggtcttcc ccttcgaagt
     1381 cggagtcgtc cgacgggcag ctgatatcgc ccgcggcggc gacggtgatc gacccgtccg
     1441 ggaggcgcgg ggaccggacc ggcgacgtcg gtcgggatcc gggatcctcg acctgcgcgc
     1501 acaccgtcag gcacaacacc acgaggacga cccacacgca tcggattcgc gctctcgcca
     1561 tcacccgccg gagggtagcg gcccccggcg tccgggacca cgaagcccct tccggtgcgt
     1621 gcaccgtgcc tcctgcgggt acggacccag cgtgcccacc ctcgggatcc atgcatcaca
     1681 cgagcagctc tcgccccacg acctcctcga ggtggtgcgg gccgctgagg cggccgggtt
     1741 cccggccgcc atgtgctccg atcacctcgc tccgtggagc gagcgtcagg gggagtccgg
     1801 ccatgcctgg gcctggctcg gcgccgcgat gcaggcgacg tcgctgccgt tcggggtggt
     1861 gaccgctccc ggacagcgct accaccccgc ggtggccgcc caggcgatcg ccacgctcgg
     1921 cgacctgttc cccggccggt tctgggccgc gctcggctcg ggcgaggcgc tgaacgagca
     1981 cgtgaccggc gacccatggc cccagaagga ggatcgggac gtccggctcc tcgagtgcgt
     2041 cgacgtgatc cgggccctcc tccgtgggga tgaggtgacc cacgacggcc tcgtgcgcgt
     2101 ggaccgcgcg cgggtgtgga gcctgcccgc cgagccgccc gccctctacg gcgccgcggt
     2161 gagcgaggag acggcgagga ccgtcgcctc ctgggccgac gggatgatca ccgtgtacca
     2221 gccgatcgac gtcctccgga gggtggtcga cgccttccgc gagggaggcg gcgatcgcgc
     2281 gccggtcgcc gtgcaggtcc acgtctcctg ggcggaggac gaggagacgg cgctcgcgat
     2341 cgcccacgac cagtggcgga cgaacgtgtt cgggtcggag ctcgcgtgga acctcgagct
     2401 gccggcgcag ttcgacgagg ccgccgcccg cgtgcggccc gacgacgtca gcgaggcggt
     2461 cctggtctcg gccgacctcg gccagcacgc gaagtggctc ctcgagctgg cggacctcgg
     2521 cgtggacgcg atctacgtcc atcacgtcgg gaaggaactg gatgccttca tcgaggcgtt
     2581 cgccgagcac gtcctcccgg agctagcatc gtgacgaccg cgcgcacgag cgatctgtgg
     2641 tggaagagcg cgatcgtcta ttgcctcgac gtagagacgt tcctcgattg ggacggagac
     2701 ggcaccggcg acctgatcgg cctcaccgag cggatcgact acctggccgg gatcgggatc
     2761 agctgtctgt ggctgatgcc cttccagccc tcgccgaacc gggacgacgg ctacgacatc
     2821 acggactact acggcgtcga cgagcggctg gggtcgttcg gcgacttcgt cgcgctgatc
     2881 cggacggcga aggaccgggg gatgcgcgtg atcatcgacc tcgtcgtgaa ccacacgtcc
     2941 gaccgccatc cgtggttccg tgcagcgcgt tcctcgcgga catctccgtt ccgggactgg
     3001 tacgtctggt ccgacgacat ccccgaggag ccgaaggccg aggtcgtctt cccggacgcc
     3061 gaggacagca tctgggcgtg ggacgagaag gcgggccagt actacctgca ccacttctac
     3121 tcgcaccagc ccgacctgaa catcgcgaac ccgaaggttc gagaggagct ggcgaggatc
     3181 gtcggcttct ggctcgaact cggcgtcgac ggcttccggg tggacgccgt gccgttcctc
     3241 ctcgagaccg gcgggatcgc cggcgacgtg gagatcgatc cccacgactt cctccgggac
     3301 ctccggtcgt tcatggcccg ccggaggggc gatgtgatga tgctcggcga ggtgaacctg
     3361 ccccacaagg atctacagct gttcttcggc ggcccggacg gcgacgaggt ccagctgctg
     3421 ttcaacttca tcctgatgca gtcgatctac ctctcgttgg cgcgccagga cgcccgtccg
     3481 ctggcgaagg cgctccgtga gacggtgcgg aagacggatg accagcagta cgcgaacttc
     3541 ctccgcaacc acgacgagct gacgctcgac aaactgaccg acgcccagcg ccaggaggtc
     3601 ttcgacgcgt tcgggccgga ggaggacatg cagctcttcg ggcgtgggct ccgccgacgc
     3661 ctcccctcga tgctcgacgg cgacgagcag cgcatccgca tggcctacag cctgctgttc
     3721 tcgctcccgg gcacgccggt gctgttctac ggcgaggaga tcggcatggg ggagaacctc
     3781 gcgatcgagg gacggttgag cgtccgctca ccgatgcagt ggaccgacga gaagaacggt
     3841 gggttctcct cggctccgcc ctcacgcctg cgccgaccgg tcgtcgaggg caggttcggg
     3901 ccgatggcgg tgaacgccgc ggcgcagcgg cgcgaccccg attcgttgct gacgtggatg
     3961 gagcgtctga tcaggcgccg gcgtgagacc cctgagctcg cgtggggggc ttggcacgtg
     4021 ctcgaaacgg atgtcgtcgg cgtgttcgcg caccggtgcg attgggaggc acgcagcgtc
     4081 gtcgccgtgc acaacctcgg tgccgacccg tgcatcgttc gcgtcgagct cggcgacgtc
     4141 cccgagggcg cgaagctcga cgacctcctg gacgagcgcg gggcgctcga cgagatcgac
     4201 ggggccgcgc tcgagctgaa gctcggtggg tacggctacc gctggttccg gatcggcaca
     4261 cccgaccagc ggaccccacc gtagacctag tcgacgatgc cggtctcgct catcacccga
     4321 tccatcggga gaggaacgcg gacctgcgtc tgtgtctgat cctcgaggaa cccgcccagc
     4381 cggatccgga aggtcgcccc acagccctcc tcgctgaaca catcgatcga accgccgtgc
     4441 gcgtcgacga tcgccttcgt gatcgacagg cccaagcctg atcccccatc gctcctggag
     4501 cgatcgatcc cgacgcgcga gaaccgatcg aagatccttg ggaggtcttc gtcgcggatc
     4561 cc
//
LOCUS       DASUVE010000100         2763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1178280, whole genome shotgun sequence.
ACCESSION   DASUVE010000100 DASUVE010000000
VERSION     DASUVE010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178280"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VF036_02045"
     CDS             complement(<1..371)
                     /locus_tag="VF036_02045"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX6264042.1"
                     /translation="MALPPFHVHLDVLATLGGLAVVYLLAAGRHEQTTGERLPRGRRY
                     RFLGGVAVLLLGATWPIHDLAESYLYSMHMVQHMLFTFVAAPLLLTGTPAWMWRAILA
                     PRPFRAAWALLTRPLVALIVA"
     gene            complement(371..700)
                     /locus_tag="VF036_02050"
     CDS             complement(371..700)
                     /locus_tag="VF036_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX6264043.1"
                     /translation="MALEQHEVELPAEHAGSHGGHPGAKEYVRIAVILFVLTGLEIGA
                     SYAGVSIGILIPTLWILAIVKFALVVLWFMHLRFDDRRYARFFVMGLAGASVLYLVVL
                     ISLRAWA"
     gene            complement(690..1322)
                     /locus_tag="VF036_02055"
     CDS             complement(690..1322)
                     /locus_tag="VF036_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HEX6264044.1"
                     /translation="MADVALAHGSDHPETTTGLPHTKLAMWIFLASECMLFGALITTY
                     VLYRGRPGQPPPHPSEIFDIPYTSVSSFVLLTSSLTMVLALSAAQDRDTGRMRLWLLT
                     TALLGLTFVGGQVYEFTAFYNEGLKLSTNLFGTTFYVLTGFHGVHVTVGVLMLLSLVG
                     LSSAERLPEDPSFPVEMVGLYWHFVDIVWIVIFTVVYLIPTPTPGGPGGP"
     gene            complement(1325..>2763)
                     /locus_tag="VF036_02060"
     CDS             complement(1325..>2763)
                     /locus_tag="VF036_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HEX6264045.1"
                     /translation="YAPNSTTDPTIGMSFYAIGLIITGIASTAGAVNLAVTVINLRAP
                     GMSLFRVPVFVWMGLVVQLLLAFSLPIITVALFQLLFDRELGTHFFDPTAGADPILWQ
                     HLFWLFGHPEVYVLILPAFGIFSEVLPVFSRKPLFGYQFVVFSGIAIGFIGFGVWAHH
                     MFAAGLGPVANTAFGVTTAIIAVPTGVKIFNWMGTIYGGDIRFTSSMLFTLGGIMMFV
                     IGGLSGVTHAIVPSDYQQTDTYYIVAHFHYVIFGGGVFAFFSAMFYWWPKVFGRLLNE
                     RLGVIEFVLMTIGFNLTFGPMHILGLQGMIRRTYTYPESLGLTFWNQAATVGAFLIAL
                     SVLLFIVNVVISHAKAKPSDVADPWDARTLEWMTTSPPPVHNFDEVPTVHARDEFWHR
                     KYVEDERTGRLVPAQAGAANGHAAEHGGHGIHLPSPSYWPLVAALGMPVIGYGVLFSW
                     WLVGVGFVIALIGFYGWAMEPSVSPEGA"
BASE COUNT          555 a          893 c          949 g          366 t
ORIGIN      
        1 ttggcgacga tcagtgccac gagcggacga gtgagcagcg cccacgcggc ccggaacgga
       61 cggggcgcca agatcgcgcg ccacatccag gcgggcgttc ccgtgagcaa gagcggtgcc
      121 gcgacgaacg tgaacagcat gtgctgcacc atgtgcatgc tgtagaggta gctctcggcg
      181 aggtcgtgga tcggccacgt cgcgcccagg agcagcacgg cgaccccgcc caggaaacgg
      241 taccggcggc cacgcgggag ccgctcccca gtcgtctgct cgtgcctccc cgcggcgagc
      301 aagtacacga cggccagccc gccgagcgtc gcgagcacgt ccaggtgcac gtggaacggg
      361 ggcagagcca tcaggcccaa gcccgcaacg agatcagcac gactagatag agcaccgacg
      421 ctccggccag gcccatcacg aagaagcgtg cgtagcgacg gtcgtcgaac ctcaggtgca
      481 tgaaccacag cacgacgagc gcgaacttca cgatcgcgag gatccacagg gtcgggatca
      541 ggatgccgat gctcacgccc gcgtacgacg cgccgatctc gagtcccgtg aggacgaaca
      601 ggatcacggc gatccgcacg tactccttcg cgcccgggtg gccgccgtgc gacccggcgt
      661 gctcggccgg cagctcgacc tcgtgctgct caagggccac cgggacctcc cggggtcggc
      721 gtcgggatca ggtacacgac ggtgaagatc acgatccaca cgatgtcgac gaagtgccaa
      781 tacagcccga ccatctcgac cgggaaggag ggatcctccg gcaggcgctc ggccgacgac
      841 aagccgacga gcgagagcag catgaggacg ccgacggtga cgtggacgcc atggaacccg
      901 gtgaggacgt agaacgtcgt cccgaacagg ttcgtcgaga gcttgagccc ttcgttgtag
      961 aacgcggtga attcgtagac ctgtccgccg acgaacgtca ggccgagcaa cgccgtggtg
     1021 agcagccaca gacgcatcct gccggtgtcg cggtcctgcg cggcggacag cgccagcacc
     1081 atcgtgagcg agctcgtgag cagcacgaac gaggacaccg acgtgtacgg gatgtcgaag
     1141 atctcgctgg ggtggggcgg cggttgccct gggcgtcccc ggtagagcac gtacgtggtg
     1201 atcagcgcgc cgaacagcat gcattcggag gcgaggaaga tccacatcgc gagcttcgtg
     1261 tgcgggaggc ctgtggtcgt ctccgggtgg tcggagccgt gcgcgagtgc gacgtcggcc
     1321 atcgctacgc gccctccggc gacaccgacg gctccatcgc ccatccgtag aagccgatca
     1381 gcgcgatcac gaacccgacc ccgacgagcc accaggagaa gagcacgccg tacccgatca
     1441 ccggcatgcc gagcgccgcc acgagcggcc agtacgaggg gctgggcagg tggatgccgt
     1501 gacccccgtg ctcggcggcg tgaccgttcg cggcgccggc ctgggccggg acgagccggc
     1561 ccgtccgctc gtcctcgacg tacttgcggt gccagaactc gtcccgcgcg tggacagtgg
     1621 gcacctcgtc gaagttgtgg acgggcggcg gcgaggtcgt catccattcg agggtccgag
     1681 cgtcccaggg atcggcgacg tcggagggct tcgccttcgc gtggctgatc acgacgttga
     1741 cgatgaacag gaggacggag agggcgatca ggaacgcgcc gaccgtggcg gcctggttcc
     1801 agaacgtgag accgagcgac tccgggtacg tgtaggtccg ccggatcatc ccctgcaggc
     1861 cgaggatgtg catgggcccg aacgtgaggt tgaacccgat cgtcatcagg acgaactcga
     1921 tcacgccgag ccgctcgttc aggaggcgcc cgaacacctt cggccaccag tagaacatcg
     1981 cgctgaagaa ggcgaacacg ccaccgccga agatcacgta gtggaagtgg gcgacgatgt
     2041 agtacgtgtc cgtctgctgg tagtcggacg ggacgatcgc gtgcgtcaca ccggacagtc
     2101 cgccgatcac gaacatcatg atcccgccca acgtgaacag catcgacgac gtgaagcgga
     2161 tgtcgccccc gtagatggtg cccatccagt tgaagatctt gacgccggtg ggcaccgcga
     2221 tgatcgccgt cgtgacgccg aaggcggtgt tggccaccgg ccccagccca gccgcgaaca
     2281 tgtggtgagc ccacacgccg aacccgatga agccgatcgc gatcccggag aacaccacga
     2341 actggtaccc gaacaggggc ttccgcgaga agaccggcag gacctccgag aagatgccga
     2401 aggcggggag gatcagcacg tagacctcag ggtgtccgaa gagccagaag aggtgctgcc
     2461 acaggatcgg gtccgccccc gccgtgggat cgaagaagtg cgtccccagc tcacggtcga
     2521 acagcagctg gaacagcgcg accgtgatga tcggcaggga gaacgcgagc agcagctgca
     2581 ccacgagccc catccacacg aacacgggca cgcggaacag cgacatcccc ggcgcccgga
     2641 gattgatgac cgtgacggcg aggttcacgg ctcccgccgt cgaggcgatc ccggtgatga
     2701 tgagcccgat cgcatagaag ctcatcccga tcgtcggatc ggtggtcgag ttcggcgcgt
     2761 agc
//
LOCUS       DASUVE010000101         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_990993, whole genome shotgun sequence.
ACCESSION   DASUVE010000101 DASUVE010000000
VERSION     DASUVE010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990993"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..2431)
                     /gene="purL"
                     /locus_tag="VF036_02065"
     CDS             complement(227..2431)
                     /gene="purL"
                     /locus_tag="VF036_02065"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012684343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX6264046.1"
                     /translation="MSSEPLHRQLGLQDDELAMIRDTLDRDPNRTELAMYAAMWSEHC
                     SYKSSKVHLRTLPTEGPAVLVGPGQDAGAVDVGDGFAAVFKMESHSHPSAIEPYQGAA
                     TGVGGIVRDIVSMGARPVALLDPLMFGPLDEERNRWLLEGVVAGIGGYGNCIGVPTVG
                     GEVRFAPPHSANPTVNVLCVGITKADRLMTAATHTPSPGSLMVLYGGTTGRDGIGGVS
                     VLASATIEEGSEGSRPSVQIGDPFAEKLLIEASLELIQDDLLEGLQDLGGAGLTCAVS
                     ESAARAGLGARLDLDRVPLREGGMEAFEILTSESQERMLAIVHPSKLEQVLAVCAKWG
                     LATAVVATLEPGGDLDVRIEGEQAAKVPALSLTEKAPMYERLMRPIRPNGDDPTFASF
                     EGDLDDALRSLLAAPNVASKRWVFEQYDRMVQGQTVAGPGSDAAVVRVPGSMKGLALS
                     VDGNGRYGSLDPYLGGAHAVAEAARNVAVTGARPLGITNCLNFGNPERPEVMWQFSEA
                     VRGMRDACLALDTPVTGGNVSFYNESSGSAIWPTPVVGVLGSLADYRLRVPSGFPIPG
                     LAVYVLGESFAELGGSEFAETVLGVVAGAPPALDLQAERRLQDLLQEAATGDLLASAH
                     DCSDGGIAVALAESSILGGHGFAVTLDGDMPPHVLLFGESASRVVVSVAPEREGSLRG
                     LAATRGVPVRRLGETGGPRAVIDGTVDATIAELTEVWEQAIPRLLGEAQSAS"
     gene            complement(2428..>2813)
                     /locus_tag="VF036_02070"
     CDS             complement(2428..>2813)
                     /locus_tag="VF036_02070"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX6264047.1"
                     /translation="SLRFVCRVVHLRVETTLTPITADVSPGEVLEIPIKHGEGQFVAA
                     DDVLDGLEAEGRVVFRYCGPDGETSDDHNPNGALRGIAGIRNQTGNVVGLMPHPEHAV
                     DPDVGPTGGQALFASLFTHVMAPAR"
BASE COUNT          424 a         1052 c          937 g          400 t
ORIGIN      
        1 gtcatattcg gccgccggac ccgccccgta ggaacgggtc gcgcgcacga tacccgaggt
       61 cagtggtgtc gacggtgacg aacccccgtg tgatgatcca gctgccgcga ctgcccagac
      121 acctccaccg aacgccaccg ccgctgcaca cagagccgct accgatcgtc gcatcgctcc
      181 gcctcccgtc agtgtcggat gagtcttcgc gtccgcatcg ttctcctcag ctcgccgatt
      241 gggcctcgcc gaggaggcgg gggatcgcct gttcccagac ctcggtgagc tcggcgatgg
      301 tcgcgtccac cgtcccgtcg atcaccgctc tcggtccgcc cgtctctccg aggcgccgga
      361 ccggcacgcc gcgggtggcg gcgagcccgc ggagcgaacc ctcccgctcc ggcgccacgg
      421 acaccacgac ccgcgacgcg gactcgccga acagcagcac gtgcgggggc atgtcgccgt
      481 cgagggtgac ggcgaacccg tgcccgccga ggatcgagga ctcggcgagc gcgacggcga
      541 tccccccgtc cgagcaatcg tgcgcagagg cgaggaggtc gcccgtggca gcctcctgca
      601 gcaggtcctg gagtcggcgc tcggcctgga ggtcgagggc cggcggcgcc cccgcaacca
      661 ccccgagcac ggtctccgcg aactccgacc ctccgagctc cgcgaaggac tcccccagca
      721 cgtacacggc cagtcccggg atcgggaatc ccgaaggcac ccggaggcga tagtccgcga
      781 gcgaaccgag cacccccacc accggcgtgg gccagatcgc cgagccgctc gactcgttgt
      841 agaagctgac gttcccgccg gtgaccggcg tgtcgagcgc gaggcatgcg tcccgcatcc
      901 cccggactgc ctcggagaac tgccacatca cctcgggtct ctccgggttg ccgaagttga
      961 ggcagttcgt gatcccgagc gggcgcgctc ccgtgaccgc gacgttccgg gcggcctcgg
     1021 cgaccgcgtg ggcgccgccg aggtagggat cgagcgatcc gtaccggccg ttcccgtcga
     1081 ccgagagggc gagccccttc atcgatcccg gtacccgtac gactgcggcg tccgatcccg
     1141 gcccagcgac ggtctgcccc tgcaccatcc ggtcgtactg ctcgaacacc catcgcttcg
     1201 acgcgacgtt gggagccgcg aggagcgagc ggagcgcgtc atcgagatcg ccctcgaacg
     1261 aggcgaacgt gggatcgtcg ccgttcggac ggatcggccg catgagccgc tcgtacatgg
     1321 gcgccttctc cgtgagcgag agagcaggca ccttggcggc ctgctcgccc tcgatccgaa
     1381 cgtccagatc cccgcccggc tccagcgtcg ccacgacggc ggtcgccagg ccccacttgg
     1441 cgcagaccgc caggacctgc tccaacttcg acggatgcac gatcgcgagc atccgctcct
     1501 gcgactccga cgtcaggatc tcgaacgcct ccatgccccc ttcccggagc ggcacgcgat
     1561 cgaggtcgag ccgcgcgccc agtcccgccc gcgctgcgga ctcgctgacg gcgcaggtga
     1621 gtcctgcgcc ccccaggtcc tggagcccct ccaacaggtc gtcctggatc agctcgagcg
     1681 acgcctcgat caggagcttc tccgcgaacg ggtcgccgat ctgcacggag gggcgcgatc
     1741 cctcgctccc ttcctcgatc gtggccgacg cgagcaccga gacgccaccg atcccgtcgc
     1801 gtccggtcgt ccccccgtac agcaccatga gggagcccgg gctgggcgtg tgcgtcgcgg
     1861 cggtcatcag ccggtcggcc ttcgtgatgc cgacgcacag cacgttcacg gtcgggttcg
     1921 ccgagtgcgg cggagcgaat cggacctcgc cgcccacggt cggcaccccg atgcagttgc
     1981 cgtagccgcc gatcccggcg accacccctt cgagcagcca ccggttccgc tcctcgtcga
     2041 gcgggccgaa catcagcgga tccagcaacg cgaccgggcg ggcgcccatc gagacgatgt
     2101 cgcggacgat cccgcccacg ccggtcgcgg caccctggta cggctcgatc gcgctcggat
     2161 gcgagtggga ttccatcttg aagacggcgg cgaacccgtc ccccacgtcg acggcaccgg
     2221 cgtcctgtcc cgggccgacg agcacggcgg gtccctctgt cggcagcgtg cggagatgga
     2281 ccttcgagga cttgtaggag cagtgttcgg accacatcgc cgcgtacatc gcgagctcgg
     2341 ttcggttggg atcgcgatcc agcgtgtcgc ggatcatcgc gagctcgtcg tcctgcaagc
     2401 cgagctgccg gtgcagaggc tcggagctca ccgcgccgga gccatcacgt gggtgaacag
     2461 cgacgcgaac agcgcctggc caccggtcgg cccgacgtcc gggtcgacgg cgtgctcagg
     2521 gtggggcatc aggccgacca cgttcccggt ctggttccgg atgccggcga tcccgcggag
     2581 cgccccgttc ggattgtggt cgtcggacgt ctcgccgtcg ggcccgcagt agcggaacac
     2641 gacccggccc tcggcctcca gcccatcgag gacatcgtcg gcagcgacga actgaccctc
     2701 gccgtgcttg atcgggatct cgaggacctc gccgggcgac acgtccgccg tgatcggggt
     2761 gagcgtcgtc tcgacgcgca gatggacgac gcggcacacg aaccggagcg atc
//
LOCUS       DASUVE010000102         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1557561, whole genome shotgun sequence.
ACCESSION   DASUVE010000102 DASUVE010000000
VERSION     DASUVE010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557561"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1351)
                     /gene="obgE"
                     /locus_tag="VF036_02075"
     CDS             complement(62..1351)
                     /gene="obgE"
                     /locus_tag="VF036_02075"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX6264048.1"
                     /translation="MTFVDEVDVEVRAGRGGDGSAAMRSEPYKPKGGPEGGDGGRGGD
                     VVFRATRNLHDLAWFADHPHLRAEPGTPGARARRDGGKGKDLVVPVPDGTVVFDERGL
                     VADLVGEGAEAVVAAGGRGGRGNTRFAGPKNRVPRTAEAGEEGDERRLHVELRTVADL
                     GLVGLPNAGKSTLLAKLTAATPKIADYPFTTLTPNLGVAEAGDERFVVADIPGLVEGA
                     HEGKGLGHRFLRHITRCRALVIVVDLSSPDAPSDLETVRAELRAYDPELADRPSLVVG
                     TKVDLVDEPARAADSIRGLPVSAVTGEGLELLATQLEQLSRQGASQLPPRAPYVVLRP
                     GRARFTISRESGGWRVVGRNVERWVHETDLDDERAVATLQRKLIKEGVERQLAKAGAR
                     RGDEIRISDRTFEFLPEVHRPEVDRPDVDPGEDRREA"
     gene            1402..1851
                     /locus_tag="VF036_02080"
     CDS             1402..1851
                     /locus_tag="VF036_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264049.1"
                     /translation="MVEPVTVWMVHLGLQAGTKPPEVRGTLTLGEEGLEFVERRSGAD
                     VRFDYASIRRAKRVRGSPVLLLDWTAGEANRKTAFYFSQPPPLEPVPRSATIDPRGPL
                     GPFTRATPSKRQVARMNLGYLRSTSVTNRQVVKAWAEAVSERMRRAS"
     gene            complement(1848..2102)
                     /gene="rpmA"
                     /locus_tag="VF036_02085"
     CDS             complement(1848..2102)
                     /gene="rpmA"
                     /locus_tag="VF036_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX6264050.1"
                     /translation="MAHKKGGGSSRNGRDSTSKRLGVKAFGGEAVTAGAIIVRQRGTR
                     IHPGDGVAKGGDDTLFALRDGTIEFHESRGRKYASVLADA"
BASE COUNT          313 a          820 c          737 g          340 t
ORIGIN      
        1 gtgagctcga gccagagccg atcctcgtcg gccaggagct cgtcgacgtt cgatctccgg
       61 ctcaggcctc ccgccgatcc tctccgggat ccacgtcggg tcgatcgacc tcgggccgat
      121 ggacctcagg caggaactcg aacgtgcggt ccgagatccg gatctcgtcg ccgcgacgcg
      181 ccccggcctt cgcgagctgc cgttcgacgc cttccttgat cagcttccgc tggagggtag
      241 cgaccgctcg ttcgtcatcg agatccgtct cgtgcaccca tcgctcgacg ttgcgcccga
      301 cgacccgcca ccctccggac tctctcgaga tcgtgaaccg cgcgcggccg ggacggagca
      361 ccacgtacgg ggcgcgagga gggagctgcg atgcaccctg tcgcgagagc tgctcgagct
      421 gggtcgcgag gagctcgagc ccctccccgg tcacggccga caccggcagg ccgcggatcg
      481 agtcggctgc gcgcgccggc tcgtcgacga ggtccacctt cgtccccacc acgagcgacg
      541 ggcgatcggc gagctccgga tcgtacgcac ggagctccgc gcggacggtc tccaggtcgg
      601 acggcgcatc cggggacgag aggtccacga cgatgacgag cgctcggcat cgggtgatgt
      661 gccggaggaa ccggtgcccg agccccttgc cctcgtgagc gccttcgacc aaccctggga
      721 tgtcggccac gacgaaccgt tcgtcgcccg cctccgcgac gcccaggttg ggcgtgagcg
      781 tggtgaaggg atagtccgcg atcttcggag tcgcggcggt caacttcgcg agcagcgtcg
      841 atttccccgc gttcgggagt cccacgaggc cgagatcggc caccgtccgc agctccacgt
      901 gcaggcgccg ctcatcgccc tcctcgccgg cttcggcggt cctgggcacg cggttcttgg
      961 gacctgcgaa gcgtgtgttg ccgcgtcccc cgcgcccacc ggcagccacc acggcttcgg
     1021 cgccctcgcc gaccaggtcg gcgaccaagc cccgctcgtc gaagaccacc gtgccgtccg
     1081 gcacgggaac cacgagatcc ttgcccttcc cgccgtcccg acgggctcgg gccccggggg
     1141 tgcccggctc ggcgcgcagg tggggatggt ccgcgaacca cgcgaggtcg tggaggttcc
     1201 gggtcgcgcg gaacaccacg tcgccgccac ggcctccgtc gccgccttcc ggcccaccct
     1261 tcggcttgta gggctcgctg cgcatcgccg cggacccgtc accaccgcga ccggcgcgca
     1321 cctccacgtc gacctcgtcc acgaacgtca tgccgaacag cctaggtggt cgacggcgcc
     1381 gccagccgcg aagattgccg cgtggtcgag cccgtcaccg tctggatggt ccatctcggt
     1441 ctccaggccg ggacgaagcc gcccgaggtg cggggaacac tcaccctcgg cgaggaagga
     1501 ctggagttcg tcgaacgcag gtctggtgcg gacgtccgct tcgactacgc atcgatccgc
     1561 cgcgcgaagc gcgtccgagg ctctcccgtc ctcctgctgg actggacggc aggtgaggcg
     1621 aaccggaaga cggccttcta cttctcgcag ccgcctccgc tggaaccggt cccccgctcc
     1681 gcgaccatcg acccacgcgg accgctcggg ccgttcacac gagcgacgcc gtcgaagcgt
     1741 caggtggcga ggatgaacct cgggtatctc cgctccacga gcgtcacgaa caggcaggtc
     1801 gtgaaggcgt gggccgaggc cgtgtccgag cggatgcggc gagcgagcta ggcgtcggcg
     1861 agcacggacg cgtacttccg gcctcgcgac tcgtggaact cgatcgtgcc gtcccggagg
     1921 gcgaacagcg tgtcgtcccc gcccttcgcg acgccgtcac ctgggtggat ccgcgtgccg
     1981 cgttgacgca cgatgatcgc gccggcggtg accgcttcac cgccgaacgc cttgaccccc
     2041 agtcgcttcg aggtcgagtc ccgaccgttc cgggacgagc cgccaccctt cttgtgtgcc
     2101 atggtccgac gaggctacca gctactcgga ggtcgccgcg gcatccttct tcgcggcggt
     2161 cgtccgcttc gccggcacct tcacctcggt gatctcgagc atcgtgagca
//
LOCUS       DASUVE010000103         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_615699, whole genome shotgun sequence.
ACCESSION   DASUVE010000103 DASUVE010000000
VERSION     DASUVE010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615699"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VF036_02090"
     CDS             <1..292
                     /locus_tag="VF036_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEX6264051.1"
                     /translation="EKLREAGVRLEDEPPPDEGPKPLEGKTIVLTGGLDTLSRDEATK
                     LAQRAGARVSSSVSKKTDFVVAGENPGAKHDQAVKLGVEIVDEQELLRRLGR"
     gene            314..1231
                     /locus_tag="VF036_02095"
     CDS             314..1231
                     /locus_tag="VF036_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEX6264052.1"
                     /translation="MAGTVIDGNALADELSEHVHEELRQLRVSGARPGLATILVGDDH
                     AAAAYERHVRRLAERLECEYANEHLPDDAELADVLAVIGKLNADPRITGILVLRPLPV
                     GISEPLVYRALDPYKDVEAVHPENAGLLAQGRPRFVPSTPASCFYLLDAYVRSTGREP
                     ESFYRGSTLVVVGRSDSVGKPAASLGLQRNTTVVTCHSRTRDLATFTQQADILIVAAG
                     VAGLVTGDMVRAGVVAVDVGIDPVKDPETGKVRFVGDIEFESVAAKAEAITPVPGGVG
                     PITDVWLIGNAVSAAAAALRVEPRFGFTT"
     gene            1279..1572
                     /gene="gatC"
                     /locus_tag="VF036_02100"
     CDS             1279..1572
                     /gene="gatC"
                     /locus_tag="VF036_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019811364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX6264053.1"
                     /translation="MAVEIDIDHVARLARLALTDEEKTQLRSQLALILEHAAKVGEVA
                     ADDVPPTAYAIPRSNVYREDVPEPSIPQGEALANAPEREDGRFKVPRIVEADR"
     gene            1569..>2053
                     /locus_tag="VF036_02105"
     CDS             1569..>2053
                     /locus_tag="VF036_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6264054.1"
                     /translation="MTEALCDLTGSELATKIAGGEVSATEVVESSLERIEAVDDEIHA
                     FLTRTPEVAQERAAELDAHRGTGAPQHAVAGIPLALKDVLATMGIRTTCGSLILENYL
                     PPYESTAWARLSGHGGVLAGKTNCDEFAMGSSNENSAYGPVRNPWDLERVPGGSSGGS
                     AA"
BASE COUNT          323 a          688 c          759 g          283 t
ORIGIN      
        1 cgagaagctc cgcgaggctg gcgtccgcct ggaggacgag ccgcctcccg acgagggccc
       61 gaagccgttg gagggcaaga cgatcgtgct gaccggagga ctggacacgc tctccaggga
      121 cgaggccacc aagctcgccc agcgggcggg agcgcgggtc tcttcgagcg tgtccaagaa
      181 gacggacttc gtcgtggcgg gtgagaatcc gggcgccaag cacgatcagg ccgtcaagct
      241 cggcgtcgag atcgtcgacg agcaggagct cctgcgacgg ctcggtcggt agtcgttcga
      301 cgggaggacg tggatggcag ggacggtgat cgacgggaac gcgctcgccg acgagctctc
      361 cgagcacgtg cacgaggagc tccggcaact ccgggtctcc ggcgcgcgcc cgggcctggc
      421 cacgatcctc gtgggcgacg atcatgccgc ggctgcgtac gagcgccacg tgcggcggct
      481 cgcggagcgt ctggagtgcg agtacgcgaa cgagcatctc ccggacgacg cggagctcgc
      541 ggacgtcctg gccgtgatcg gcaagctcaa cgccgacccg cgcatcaccg gcatcctcgt
      601 actccggccg ctgcccgtcg gcatctcgga gccgctcgtg tatcgcgcgc tggacccgta
      661 caaggacgtc gaggccgtcc atcccgagaa cgcggggttg ctggctcagg gccgtccgcg
      721 gttcgtgccg tcgacgcccg cgtcgtgctt ctacctcctg gacgcctacg tgcgctccac
      781 gggtcgcgag ccggagtcgt tctatcgggg cagcacgctc gtggtcgtgg gtcggtcgga
      841 ctccgtgggc aagccggcgg cgtcgctcgg gctccagcgg aacaccacgg tggtgacgtg
      901 ccactcgcgc acgagggatc tcgccacgtt cacgcagcag gcggacatcc tgatcgtggc
      961 tgcgggtgtg gcgggcctgg tgaccggcga catggtgcgc gcaggcgtgg tcgcggtgga
     1021 cgtcgggatc gatcccgtga aggaccccga gacggggaag gtgcggttcg tgggcgacat
     1081 cgagttcgag agcgtcgcgg cgaaggccga ggcgatcaca cccgtcccgg gaggcgtggg
     1141 accgatcacc gacgtctggc tgatcgggaa cgcggtgagc gccgccgcgg ccgcgctccg
     1201 cgtcgagccc cggttcggct tcacgacgtg agcgccggcg caggtccctg aggagcgccg
     1261 tcgatagact ccggcgacat ggcggtcgag atcgacatcg atcacgtcgc gcggctcgcg
     1321 cggctcgcgt tgaccgatga ggagaagacg cagctccgga gtcagctcgc cctgatcctg
     1381 gagcacgcgg cgaaggtcgg cgaggtcgcg gccgacgacg ttcccccgac cgcgtacgcg
     1441 atcccgcgat cgaacgtcta ccgagaagac gtccccgagc cgtcgatccc gcaaggggag
     1501 gcgctcgcga acgcaccgga acgggaggac ggacggttca aggtccctcg gatcgtcgag
     1561 gcggaccgat gaccgaggcc ctctgcgacc tcaccggttc agagctcgcg acgaagatcg
     1621 cggggggaga ggtctccgcg acggaggtcg tggagtcctc gctcgagcgc atcgaggcgg
     1681 tcgacgacga gatccacgcg ttcctcacgc ggactcccga ggtggcccag gaacgcgcgg
     1741 ccgagctgga cgcgcatcgc gggaccggcg cgccgcagca cgcggtcgcc ggcatcccgc
     1801 tcgcgctgaa ggacgtgctc gccacgatgg ggatccgcac cacctgcggg tcgttgatcc
     1861 tcgagaacta cctcccgccg tacgagtcga ccgcctgggc tcgactgtcc ggccacggtg
     1921 gggtcctcgc cggcaagacg aactgcgacg agttcgcgat gggctcctcc aacgagaact
     1981 cggcctacgg cccggtacgg aacccctggg acctcgagcg tgtcccggga ggctcgagcg
     2041 gcggcagcgc cgc
//
LOCUS       DASUVE010000104         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1370228, whole genome shotgun sequence.
ACCESSION   DASUVE010000104 DASUVE010000000
VERSION     DASUVE010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370228"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="VF036_02110"
     CDS             <1..1590
                     /locus_tag="VF036_02110"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX6264055.1"
                     /translation="DLTDPADQALWGYAKNWRSGMVQHDRATTESELDTIVASAVVLD
                     PLLGVLKADIGIKDGRIAGIGRAGNPDITDGVDLTIGPNTWPIPCHGLIATPGAVDSH
                     VHLLSPRLVPVALSAGVTPLITAGFEEPPWRMLRTLEAFEFLPVNLGLQPSARTGVPG
                     QLEVAIEAGAVGLKIHEDWGAYPEVIDEVLRVADAHDVQVCLHTDGLNESCELADTVD
                     AIAGRAIHAYHVEGSGGGHIPDLIGIVREPNVFCSSTTPTIPYGAATAAEQQDMKLIV
                     HEGNPALADDIVAARELVHPATMEAEGPLHDLGAISIVNSDSQGMGRIGETVRRTFQL
                     AHAMKAAAIQTEGGNDRDDNDRDDNDRVLRYLAKVTEEPARVHGISDDVGSLSPGRLA
                     DLVLWEASHFGVKPVMVLKGGVVAWTAMGEGNASVHGAEPTRYGPDWGSTGLAPARVS
                     TTFVSAAAIDAGIARTLGTRRRLVAVRGTRAIRRDGLARNRTVPPIEVSVTDGTVTLD
                     GRVLRTDPITEVRLSRRYLLA"
     gene            complement(1595..2017)
                     /locus_tag="VF036_02115"
     CDS             complement(1595..2017)
                     /locus_tag="VF036_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017566036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HEX6264056.1"
                     /translation="MVAHPKRRPSVDCASVSGETTVFDAAGGMPFFEQLVARFYEGVA
                     GDPVLRPLYPDDLAPPTRHLTLFLAQYWGGPRTYDEERGHPRLRMRHAPFAVGPAERD
                     RWLVHMRAAVATMAPPEPVERALVEYFEMAAEAMRNRD"
     gene            complement(1984..>2645)
                     /locus_tag="VF036_02120"
     CDS             complement(1984..>2645)
                     /locus_tag="VF036_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264057.1"
                     /translation="LGRLDLEGELRDLGDPPGIFQAPVWLADGRMFYAIAERDRQTLV
                     VRDGDRLRELVEFRGAIEFVVDPRGERIAYRVDEGAGPGGVEVVDTTTARSQVVSASP
                     TFAFQWSPDGERLLLLTPDEGGGGGARWLVWDGDVARPVGPAFVPSPSFLRDYVPFSG
                     QFAQAMTPWSPDGGAFAFTGLIGDRAGVWVQDLRAADPTFVLEGGSVVAWSPTRSAAR
                     P"
BASE COUNT          426 a          981 c          898 g          340 t
ORIGIN      
        1 gacctcacgg acccggccga ccaggcgctc tggggctacg cgaagaactg gcgatcgggg
       61 atggttcagc acgatcgcgc gaccaccgag agcgagctcg acaccatcgt cgcgagcgcg
      121 gtcgtgctgg accctctcct cggcgtcctc aaagcggaca tcggcatcaa ggacggccgg
      181 atcgccggga tcggcagggc gggcaacccg gacatcaccg acggcgtcga tctcacgatc
      241 gggccgaaca cgtggccgat cccgtgccac ggtctgatcg ccaccccggg tgccgtcgac
      301 tcgcacgtgc acctgctctc ccccaggctc gtcccggtcg cgctctcggc cggcgtcacc
      361 ccgctcatca ccgcggggtt cgaggaaccc ccgtggcgca tgctccgcac cctggaggcc
      421 ttcgagttcc tccccgtgaa cctcgggctc cagccgtcgg cccggaccgg ggtccccggg
      481 cagctcgagg tggcgatcga ggcgggcgcc gtcggcctca agatccacga ggactggggc
      541 gcctaccccg aagtgatcga cgaggtgctc cgcgtcgcgg acgcgcacga cgtccaggtc
      601 tgcctgcaca cggacgggct gaacgagtcg tgcgagctcg cggacaccgt ggacgcgatc
      661 gccggtcgtg cgatccacgc gtaccacgtc gagggctcag gtggcggcca catcccggac
      721 ctgatcggga tcgtccgcga gccgaacgtc ttctgctcgt cgaccacacc gacgatcccc
      781 tacggcgccg cgaccgcggc cgagcaacag gacatgaagc tcatcgtgca cgagggcaat
      841 cccgcgctcg ccgacgacat cgtggcggct cgcgagctcg tgcatcccgc gacgatggag
      901 gccgaaggac cgctgcacga tctcggcgcg atctcgatcg tgaactcgga ctcacagggg
      961 atgggtcgga tcggcgagac cgtgcggcgg acgttccagc tcgcgcacgc catgaaagcg
     1021 gcggcgatcc agaccgaggg cgggaacgac cgcgacgaca acgaccgcga tgacaacgac
     1081 cgcgtccttc gctacctcgc gaaggtcacc gaggagccgg cacgtgtgca cggcatctcg
     1141 gacgacgtcg gctccctctc ccccggacgg ctcgccgacc tggtcctgtg ggaggcgtca
     1201 cacttcgggg tcaagccggt gatggtgctc aaaggagggg tcgtcgcgtg gaccgcgatg
     1261 ggcgagggca acgcgagcgt gcacggcgcg gagcccaccc ggtacggacc cgattggggg
     1321 agtacggggc tggcgccggc gcgtgtctcc acgacgttcg tctcggcggc cgcgatcgac
     1381 gcggggatcg cccggacgct cggcacacga cggcggctcg tggccgtccg cgggacccgc
     1441 gcgatccggc gtgacggcct cgcgcggaac cggaccgtcc cgccgatcga ggtctcggtc
     1501 accgacggaa cggtgaccct cgacggacgg gtgctgcgga ccgatcccat aaccgaggtg
     1561 cggttgtcgc ggcgctacct gctcgcgtga gcgatcagtc ccggttccgc atcgcctcgg
     1621 cggccatctc gaagtactcc accagcgccc gctcgaccgg ctccgggggg gccatcgtcg
     1681 cgacggccgc ccgcatgtgc accagccagc ggtcgcgctc ggcgggtccc accgcgaacg
     1741 gggcgtgccg catccgcaac cgagggtggc cccgctcctc gtcgtacgtc ctgggcccgc
     1801 cccagtactg ggcgaggaag agcgtcaggt gccgggtggg cggcgcgaga tcgtccggat
     1861 acaagggccg cagcacggga tcgcccgcga cgccttcgta gaaccgcgcc acgagctgct
     1921 cgaagaacgg catgcccccg gcggcatcga acacggtcgt ctcacctgac acggacgcgc
     1981 agtctacgga cgggcggcgc ttcgggtggg cgaccacgcc accacggagc cgccttccag
     2041 cacgaacgtg ggatcggcgg cgcggagatc ctgcacccac acccctgccc gatcgccgat
     2101 cagcccagtg aacgcgaacg caccgccgtc cggagaccac ggcgtcatcg cttgcgcgaa
     2161 ctgtccggag aacgggacgt agtcccgcag gaaggacggg ctcggcacga acgccgggcc
     2221 gaccggccgc gccacgtcgc cgtcccacac cagccatcgg gcgccgcccc caccgccttc
     2281 gtccggggtc agcagcagga gccgctcgcc atctggactc cactggaacg cgaacgtcgg
     2341 cgacgccgag acgacctgcg aacgcgccgt cgtcgtatcg accacctcca ccccacctgg
     2401 tccggcgcct tcgtccacgc ggtacgcgat ccgctccccg cggggatcca ccacgaactc
     2461 gatcgcgccc cggaactcga ccagctcacg gagccggtcc ccgtcgcgca ccacgagcgt
     2521 ctgtcgatcc cgctcggcaa tggcgtagaa catccggccg tccgcgagcc agacgggagc
     2581 ctggaagatg cccggcggat cgccgaggtc tcgcagctcc ccttcgagat ccaatcgccc
     2641 gagcg
//
LOCUS       DASUVE010000105         3458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1370581, whole genome shotgun sequence.
ACCESSION   DASUVE010000105 DASUVE010000000
VERSION     DASUVE010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370581"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1192)
                     /locus_tag="VF036_02125"
     CDS             complement(98..1192)
                     /locus_tag="VF036_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6264058.1"
                     /translation="MSLRATVAADRGSFEVRAAIAAQEGETLALLGPNGAGKTTVVLA
                     LAGLIDSIGAIELGDERIDGLPPEGRPLGVAFQNGALFPHMSTLANVAFPLRARGIRR
                     RAAHEEASAILARLAPDVRPEALPPTLSGGEAQRVALARAIVHEPRLLLLDEPSSGLD
                     VRGRAELRPVLVDALASFPGVRVLVTHDPVEAMTLADRVAILERGRLTQEGTFDDVRR
                     TPRSRYAAELVGLNLFVGRLEPIDGGAGRLVTPEGDVVVGWPEGAARRPIDGVTGTLR
                     PLDVVVHTELPAQSSARNVWRGTVEDLAIYGERVRARIASRPPLVAEVTAGSVRQLGL
                     VRGSEVWASCKAVEVEITLPASEGTRTYPG"
     gene            complement(1189..1953)
                     /gene="modB"
                     /locus_tag="VF036_02130"
     CDS             complement(1189..1953)
                     /gene="modB"
                     /locus_tag="VF036_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX6264059.1"
                     /translation="MGVVVLAALGAAFVLLPIAGLLDRAPWDQAAELLSSDIAVDGLR
                     VSLLVSFGAAAISILVGVPLGLLLGRTSFPGIALVRALALLPLVLPPVVAGIGLLTAF
                     GRRGLLGGPARAIGLELPFTTAGAAVAAAFVSFPFVVLAVEAGLRSLDTRLEDAAASL
                     GGSRWYVLRRVTLPMLAPQLVAGTVLAWARALGEFGATITFAGNFRGETQTMPLAVFE
                     ALQSDPGAATLLSLVLVVASLGILIALRGRFIGAPT"
     gene            complement(1968..2717)
                     /gene="modA"
                     /locus_tag="VF036_02135"
     CDS             complement(1968..2717)
                     /gene="modA"
                     /locus_tag="VF036_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6264060.1"
                     /translation="MRARALLASLLIVTTACGRTDPRDAPNSVTVFAAASLTEAFTQI
                     ADEYQELHGVEVRLSFGPSDGLATQIQEGAPADVFASASLKWMDAVAEDPGVTDRADF
                     ARNVLVVIVPARDPAGIVTVDDLGEPGIKLILAAEGVPVGDYAREMLANAGIADEALA
                     NVVSNEDDVKGVVQKVALGEADAGIVYRTDVTASVAEDLVSNDVPDDVNVVAFYPIAA
                     LAGASEHARGFVEYVLGPGQETLRAAGFLPP"
     gene            complement(2714..3100)
                     /locus_tag="VF036_02140"
     CDS             complement(2714..3100)
                     /locus_tag="VF036_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX6264061.1"
                     /translation="MDDYRIGQAADAIGVRVETLRRWERDGKLSTVRTAGGQRRVPAA
                     EVARLVAERRAERAPASSSRRNAFPGVVTKVTRDTVSALVEIQAGPHRVVSLITREAA
                     DELDLKPGMGAVATVKATSVMIEVEK"
BASE COUNT          539 a         1211 c         1207 g          501 t
ORIGIN      
        1 cgccgaggaa cgtgagcgcc gggtggcgac gcttcgtggg ccgcgcggtc tcgccgtcta
       61 cgtccgtctt ccggagggag cgctcatccg ttttcactta cccagggtac gtcctcgttc
      121 cctcggacgc gggcagcgtg atctccacct cgaccgcctt acaggaggcc cagacctcgg
      181 agccccgaac caggccgagc tggcggacgg agccagccgt gacctcggcc acgagtgggg
      241 gtcgcgaggc gatcctcgct cggacgcgct ctccgtagat cgcgagatcc tccaccgtcc
      301 cgcgccagac gttgcgggcg gagctctgcg ccggcagttc ggtatgcacg accacgtcta
      361 acggtcgcaa tgtcccggtc accccgtcga tcggccggcg agcggctcct tccggccagc
      421 cgaccaccac gtcgccctcg ggggtgacga ggcgccccgc cccaccgtcg atcggctcca
      481 gccgcccgac gaacagattg aggccgacca gctcggctgc gtaccgactt cgcggggtac
      541 ggcgcacgtc gtcgaacgtt ccctcctggg tgagccgccc gcgttcgagg atcgccacgc
      601 gatcggcgag ggtcatcgcc tccacggggt cgtgggtcac gagcacgcgg accccgggga
      661 acgacgccag tgcgtcgacg agaaccggac ggagttccgc tcggcctcgg acgtcgagac
      721 ccgaggaggg ttcgtcgagg aggaggagtc ggggctcgtg gacgatggcg cgagcgagcg
      781 ccactcgctg cgcctcgccc ccggaaagcg tcggtggcag tgcctcgggt cgaacgtcgg
      841 gcgcgaggcg agcgaggatg gcgctcgcct cctcatgcgc tgcgcggcgt cggatgccac
      901 gggcgcggag cgggaacgcc acgttcgcga gagtgctcat gtgggggaag agggcgccgt
      961 tctggaacgc gaccccgagg ggccgtccct ccggggggag cccgtcgatc cgttcgtctc
     1021 cgagctcgat cgcaccgatg ctgtcgatga gcccggcgag tgccaggacg accgtggtct
     1081 tccccgcacc gttcggtccg agcagcgcga gcgtctcgcc ctcctgagcc gcgatcgccg
     1141 cgcgaacctc gaacgaccct cggtccgccg cgaccgttgc ccgcaggctc atgtcggcgc
     1201 cccgatgaac cgacctcgca gcgcgatcag gatgccgagc gacgcgacca caaggaccag
     1261 ggagaggagg gtcgcggcac cgggatccga ctggagcgcc tcgaacactg cgagcggcat
     1321 cgtctgggtc tcccctcgga agttgcccgc gaacgtgatg gtggcgccga actccccgag
     1381 cgcgcgcgcc caggccagca ccgttcccgc cacgagctgc ggggccagca tcgggagggt
     1441 gacccgtcgg aggacgtacc acctcgaccc accgagggag gcggcggcgt cctcgaggcg
     1501 ggtgtcgagc gagcggagcc cggcttcgac ggcgagcacg acgaacggga acgacacgaa
     1561 ggcggccgcc accgcagctc ccgccgtggt gaacgggagc tccaagccga tcgcgcgcgc
     1621 cggtcctcct aggagtcctc ggcgcccgaa cgccgtgagc agcccgatcc cggcgacgac
     1681 cgggggaagg acgagcggga ggagggcgag tgcgcgcacc agcgcgatcc ctgggaacga
     1741 ggtccggccg agcagcaggc cgagcggaac cccgacgagg atcgagatcg ccgcggcgcc
     1801 gaacgacacg agcaaggaca cgcggagccc gtcgaccgcg atgtcggagg agaggagctc
     1861 tgccgcctgg tcccagggcg ctcggtcgag caatccggcg atcggcagga gtacgaacgc
     1921 cgccccgagg gccgcgagga cgacgacccc cacggggagc cggcgtctca cggtgggagg
     1981 aagcccgccg cccggagcgt ctcctgcccg ggaccgagga cgtactcgac gaacccccga
     2041 gcgtgctcgg aggcaccggc gagggcggcg atgggataga aggcaacgac attgacgtcg
     2101 tccggcacgt cgttcgacac gaggtcctcg gcgaccgagg cagtgacgtc cgtccggtag
     2161 acgatgccgg cgtcggcttc tccgagagcc accttctgga cgaccccctt gacatcgtcc
     2221 tcgttcgaca ccacgttcgc gagggcctcg tccgcgatcc ccgcgttcgc gagcatctcg
     2281 cgggcgtagt ctccgacggg cacgccttcg gctgcgagaa tcagcttgat gcccggttcc
     2341 ccgagatcgt cgaccgtgac gatgccggcg ggatccctgg ccggaacgat gacgacgagg
     2401 acgttacgtg cgaagtccgc ccggtcggtg acgccggggt cctccgccac cgcgtccatc
     2461 cacttcaggc tcgccgaggc gaacacgtcc gccggagcgc cttcctggat ctgggtcgcg
     2521 aggccgtcgg acgggccgaa gctgagacgg acctcgacgc catggagctc ttggtactcg
     2581 tcggcgatct gcgtgaacgc ctcggtgagg gatgccgctg cgaataccgt gacggagttg
     2641 ggcgcgtcgc gcgggtcggt gcgtccacac gcggttgtga cgatcaacag cgaggcgagg
     2701 agggcgcgtg ccctcacttc tccacctcga tcatcacgct ggtcgccttc acggtggcga
     2761 cggcacccat gcccggcttg aggtcgagct catcggccgc ctcacgggtg atcagtgata
     2821 cgacccggtg cggtccggcc tggatctcca ccagcgccga gaccgtgtcg cgcgtgacct
     2881 tcgtgacgac cccggggaac gcgttccgtc ggctggagga agccggcgct cgctcggctc
     2941 gtcgctccgc gacgagccga gccacctccg ccgccgggac gcggcgctga cctcccgcgg
     3001 ttcggaccgt gctcaacttc ccgtcccgtt cccaccgccg gagcgtctcg acccgtaccc
     3061 cgatcgcgtc cgccgcctgc ccgatccggt agtcgtccat ggaaccgtgc agtatacagg
     3121 ttcgagtggg tctatcgata gaaaaaccca gctactcggg gtttctaggc tgaccctgtc
     3181 gttgagtctt caacttgtag cacgatgcgc ggctatgtct gccgggcaca tcgctccgat
     3241 cagaagggat cgctgagccg tgctccgtgc cgtccgcgcc ttcctggatc aagcacccga
     3301 agcttcgacc ttggtcgctg aagttcaagg acgagtggct cgagcgccgg ttccggacac
     3361 ctacttggac gccaccatcc agtacatccg gatcgcgacg ttcctgggcg cgcgttcggt
     3421 ccgctcgcgt tgctggtcgt cgacggggaa ggcgtcct
//
LOCUS       DASUVE010000106         3273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_804560, whole genome shotgun sequence.
ACCESSION   DASUVE010000106 DASUVE010000000
VERSION     DASUVE010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804560"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /gene="cysC"
                     /locus_tag="VF036_02145"
     CDS             complement(<1..516)
                     /gene="cysC"
                     /locus_tag="VF036_02145"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX6264062.1"
                     /translation="MTATERNGFTVWFTGLSGSGKSTIAEMLFHEFQARGLKTEILDG
                     DVVRQNLSKGLGFSKEDRDTNILRIGFVADLLTRNGVATIWCPISPYKAARDAVRAQI
                     GEAFVEVYVHATVDEIAANRDPKGLYKKALAGEITGFTGVDDPYEAPESPELVVDTLV
                     ETPEQSLQKVMD"
     gene            complement(513..1907)
                     /locus_tag="VF036_02150"
     CDS             complement(513..1907)
                     /locus_tag="VF036_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6264063.1"
                     /translation="MQQGPRVAVIGLDCGTPQLLFDDLAAEVPNISKLMAEGMYGDLA
                     SITPPITVPAWACAMTGRTPGELGIYGFRNRKDTSYEGLSIAHSGSIGAPAVWDSLGE
                     EGKSSLLIGVPPGFPPPKEFPGWRVGCFLTPPSAEHFAYPQELEIEIEEELGGKGQYI
                     FDITNFRKQGMDFVLDQVFKMTERRFKVARRLVREKPWDFFMLVEMGMDRLHHVFWHY
                     FDPKHPLYKPGNPFEDAFQRYYRALDDEVGTLLELLPEDVITIVMSDHGARPMMGGVC
                     FNDWLIEQGYLVLKEPVSGVTPVARTAIDWGRTTAWGDGGYYGRLFLNVAGREPQGSI
                     QPAEYETVRNELVTKLEAMPGPDGALLGTKVHKPQDVYPEVKGVAPDLLVYFGDLEWR
                     SVGSVGNPSIYTFENDIGPDGANHDRTGVFAMKGAPGQPSGKVDGLSLVDVGPTILSL
                     YGIESHGAASRSFL"
     gene            2076..2471
                     /locus_tag="VF036_02155"
     CDS             2076..2471
                     /locus_tag="VF036_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264064.1"
                     /translation="MTVLRRALGLGTLLLLVPAVLLVAVPRTIVERVMGQSAGDDVWI
                     RLTGAACIALALLHVLVIRKLDDLWWWCWAFVLFDATVAAISLTHAAVGLPEDSAAWP
                     WWLLGTTAAGFAALYLVGLAKAGREQPFA"
     gene            complement(2480..2965)
                     /locus_tag="VF036_02160"
     CDS             complement(2480..2965)
                     /locus_tag="VF036_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6264065.1"
                     /translation="MSVTEIRSLEPDDAPACDAIVAGLPYHFGQEQGRRDCAAAVRHD
                     PGLVAVEGREITGFLTYVHRFDEAAEITWMAVRADRRRQGIGHALIDRLADELKAAGR
                     RTLLVLTVSPSDPGPEPDDGYQSTRAFYRSAGFVLARDLPREWDGGDTAVLLVMSLAA
                     R"
     gene            complement(2962..>3273)
                     /locus_tag="VF036_02165"
     CDS             complement(2962..>3273)
                     /locus_tag="VF036_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid dehydrogenase"
                     /protein_id="HEX6264066.1"
                     /translation="IPLLRCRAIAGAANNQLATTEDGDRLREAGILFAPDFVVNAGGV
                     IHLAGRETLGWDDATTASRLEAIGDSLLEIFDRAEREALSPATAADRVARERVAVARG
                     E"
BASE COUNT          484 a         1151 c         1085 g          553 t
ORIGIN      
        1 gtccatgacc ttctgcagcg actgctccgg ggtctcgacg agcgtatcca cgaccagctc
       61 cggactctcg ggtgcttcgt agggatcgtc cacgcccgtg aagccggtga tctcccctgc
      121 gagcgccttc ttgtacaggc ccttgggatc ccgattcgcg gcgatctcgt ccacggtcgc
      181 gtgcacgtag acctcgacga acgcctcgcc gatctgggcg cgcacggcat ctcgagccgc
      241 cttgtagggc gagatcgggc accagatcgt ggcgaccccg ttccgggtga ggaggtccgc
      301 gacgaacccg atgcgcagga tgttcgtgtc gcgatcctcc ttggagaagc cgaggccctt
      361 cgagaggttc tggcggacga cgtcgccgtc gaggatctcg gtcttcaacc cccgcgcctg
      421 gaactcgtgg aagagcatct cggcgatcgt cgacttcccg gagccggaga gccccgtgaa
      481 ccagacggtg aatccgttcc gctccgtcgc cgtcacagga agctcctcga ggcggctccg
      541 tgggactcga tcccgtagag cgacaggatg gttgggccca cgtcgacgag ggagagcccg
      601 tccacctttc cgctgggctg cccgggcgct cccttcatcg cgaacacccc cgtccggtcg
      661 tggttcgcgc cgtccggtcc gatgtcgttc tcgaacgtgt agatgctcgg gttccccacc
      721 gagcccacgg aacgccactc cagatccccg aaatacacca agagatcggg cgccactccc
      781 ttgacctccg ggtagacgtc ctgcggcttg tgcaccttgg tgccgagcaa cgctccgtcg
      841 ggccccggca tcgcctcgag cttcgtcacg agctcgttcc gcacggtctc gtactcggcc
      901 ggctggatcg agccctgcgg ctcccgcccg gccacgttca ggaacaaccg gccgtagtag
      961 ccgccgtcac cccacgccgt cgtgcgtccc caatcgatcg cggtccgcgc gaccggtgtc
     1021 acgccgctga ccggctcctt caacacgagg tagccctgct cgatcagcca gtcgttgaag
     1081 cagacacctc ccatcatcgg ccgcgccccg tggtcgctca tcacgatcgt gatcacgtcc
     1141 tccggcagga gttccaacaa cgtgccgacc tcgtcgtcga gggcccggta gtagcgctgg
     1201 aacgcgtcct cgaaggggtt cccgggcttg tacagcgggt gcttcgggtc gaagtagtgc
     1261 cagaagacgt ggtgcagccg gtccatcccc atctcgacca gcatgaagaa gtcccacggc
     1321 ttctccctca cgagacggcg cgccaccttg aaccggcgtt ccgtcatctt gaacacctga
     1381 tcgagcacga agtccatgcc ctgcttgcgg aagttcgtga tgtcgaagat gtactgcccc
     1441 ttgcccccga gctcctcctc gatctcgatc tcgagctcct ggggataggc gaagtgttcg
     1501 gccgacggcg gcgtgaggaa gcaccccaca cgccagcccg ggaactcctt cggtggcggg
     1561 aagccggggg gaaccccgat cagcagcgag gacttgccct cctcgccgag ggagtcccag
     1621 acggccgggg cgccgatcga tcccgagtgc gcgatgctca ggccctcgta gctggtgtcc
     1681 ttgcgattgc ggaagccgta gatcccgagc tcgcccggcg tccggccggt catcgcgcac
     1741 gcccatgccg gcacggtgat aggaggcgtg atgctggcga gatccccgta catgccctcg
     1801 gccatcagct tcgagatgtt cgggacctcg gccgcgagat cgtcgaacag gagctggggt
     1861 gttccgcaat cgaggccgat caccgcgacc cgagggccct gctgcatgcg ccgacatcct
     1921 ccctgtgttc gttgcttcgg cccgtcccgt gcgccggccc gagcgtcgga aaccgtgtgg
     1981 gccgggcgcg ccggaacgcg gggcggctca acggttctcg tatgctatca acccgcccga
     2041 aacgccgacc aagccctccc ggagatgtcc gttcgatgac cgtgctgcgc cgcgcgctgg
     2101 gcctcggaac cctcctcctg ctggtgccgg ccgtactcct tgtcgcggtg ccgcgcacga
     2161 tcgtcgagcg ggtcatgggt cagtccgccg gggacgacgt gtggatccgg ttgacgggcg
     2221 cggcgtgcat cgccctcgcg ttgctccacg tgctggtgat caggaagctc gacgacctct
     2281 ggtggtggtg ctgggccttc gtgctcttcg acgcgacggt cgcggcgatc tcgctcacgc
     2341 acgcggcggt cgggctgccg gaggactcgg cggcgtggcc gtggtggctc ctcggaacca
     2401 cggccgccgg gttcgccgcg ctctacctgg tcggtctggc gaaggccggc agggagcagc
     2461 cgttcgcctg acccgagggt caccgcgcgg ccaggctcat gacgagcagc acggcggtgt
     2521 cgcccccgtc ccactcgcga gggagatccc tggcgaggac gaaaccggcc gaccgataga
     2581 acgctcgcgt cgactggtag ccgtcgtcgg gctcgggacc gggatcgctc ggtgagaccg
     2641 tgaggacgag cagcgtccgg cgtcccgccg ccttcagctc gtccgcgagc cggtcgatca
     2701 gcgcgtgccc gatgccctga cgtcgtcgat cggcgcggac agccatccat gtgatctccg
     2761 cggcctcgtc gaaacggtgc acgtaggtga ggaaacccgt gatctcgcgt ccttcgacgg
     2821 cgacgagacc ggggtcgtgt cggaccgcgg ccgcgcagtc ccgacggcct tgctcctgac
     2881 cgaagtgata cgggagcccg gccacgatcg catcgcaggc cggtgcgtcg tccggctcga
     2941 gcgagcggat ctcggtgacg ctcactcgcc gcgcgcgacc gcgacgcgct cgcgcgccac
     3001 ccgatccgct gcggtggccg gcgagagagc ctctcgttcc gctcggtcga agatctccag
     3061 gagcgagtcg ccgatcgcct cgagccgcga agcggtcgtg gcgtcgtccc agccgagtgt
     3121 ctcgcgaccg gcaagatgga tcacgccgcc cgcgttcacg acgaagtccg gggcgaagag
     3181 gatccctgct tcgcggagcc gatcgccgtc ctccgtggtc gcgagctggt tgttcgccgc
     3241 gccggcgatc gcgcggcagc gcagcagcgg gat
//
LOCUS       DASUVE010000107         4011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_242288, whole genome shotgun sequence.
ACCESSION   DASUVE010000107 DASUVE010000000
VERSION     DASUVE010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242288"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1693)
                     /gene="uvrB"
                     /locus_tag="VF036_02170"
     CDS             complement(<1..1693)
                     /gene="uvrB"
                     /locus_tag="VF036_02170"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX6264067.1"
                     /translation="MPPFKITSEFRPSGDQPEAIDRLVDGVQAGQEHVTLLGATGTGK
                     TFTVAHLVERVQRPTLVIAPNKSLAAQLANEFREVFPENAVEYFVSYYDYYQPEAYVP
                     QTDTYIEKDSSVNDEIERLRHSATAALLSRRDVLIVASVSCIYGLGSPEQYEGQLLRT
                     VRGTDLPMEFAMQRLVDIQYQRNNVSRARGTFRVQGDTLEVFPSYEQVGYRVEWWGET
                     IERISRFDPLTGELLTDTIADLTTFPASHYVASDERMERALVTIEEELHERLAELESQ
                     SKLLEAERLRMRTTYDLEMLREVGFCSGIENYSRHIDGRAAGSAPYTLLDYFPDDYLV
                     VIDESHVTVPQLHGMYEGDISRKSTLVDFGFRLPSAIDNRPLRFEEFKERVHQIVYMS
                     ATPGPWELKVSDRIVEQIVRPTGLIDPEVEIRPTKGQVDDLIEEIRRRAEAEQRVLVT
                     TLTKKMAEDLTDYLIEMGIRVRYLHSEVDTIQRIEIIRDLRLGEFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSETSLIQTIGRAARNVDGRVILYADTITGSMERALA"
     gene            complement(1749..2495)
                     /locus_tag="VF036_02175"
     CDS             complement(1749..2495)
                     /locus_tag="VF036_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020137595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX6264068.1"
                     /translation="MTEAVRVDKVRKSFGELQVLRDVDLVVQDHEVVCLIGASGSGKS
                     TLLRCIDLLEEVDAGRIWVEGFEITGGGVDQNAVRRRIGIVFQAFNLFPHMTVMRNVT
                     LAQEKALGRTPADARAKATELLSRFGLIEKAEEYPDRLSGGQQQRVAIARALAMGPDL
                     LLLDEVTSALDPVLVAEVLDAIRELAAGGMTMVIATHEMGFARDVANRVAFLEEGRIL
                     EEGPPERMFSEPREEATRRFLARIIAAGRM"
     gene            complement(2492..3364)
                     /locus_tag="VF036_02180"
     CDS             complement(2492..3364)
                     /locus_tag="VF036_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6264069.1"
                     /translation="MTEPPEALTGHDRRSRLLGNRGFLISTASTIVFFAVVTVILVAA
                     PGFDSVRESFFNLEAMKESFFGVPEEGLPGIGQAFLLNIRIFLIAEVLILILALVIAV
                     IRSLQGPVFFPFRLIAIVYTDLFRGMPLLLVIYLFGFGLPALGIRGLSTQSEVVYATA
                     ALVLSYSAYVAEVYRAGIESVHPSQVAAARSLGLSRMQSLRFVVLPQAVRRIIPPLLN
                     DFVSLQKDTALLAVLGVVEAARAAQIYSAYRFNYSSFVVAAFLFILMTIPLARLTDWL
                     IARDRRRRQARGTP"
     gene            complement(3384..>4011)
                     /locus_tag="VF036_02185"
     CDS             complement(3384..>4011)
                     /locus_tag="VF036_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6264070.1"
                     /translation="EAALTYAVAERMGFADSQVNWVNVPFNSSYAPGPKDFDFDINNI
                     SVTEERDQAVDFSDGYYDLTQALMVLKGSPIENATTMEEIKDSVFGAQIGTTSLSFIN
                     TVIQPTVDPRVFDSTSDAKAALRNGSVDGLVLDLPTAYFEATINTPNGALVGQFPNAG
                     EYLGILFEEGSPLVECVNLALAEMTADGTLEDLQSEWLADYLAVPVIQ"
BASE COUNT          662 a         1340 c         1342 g          667 t
ORIGIN      
        1 cggcgagcgc gcgctccatc gatccggtga tcgtgtcggc gtaaaggatg acgcgcccat
       61 cgacgttgcg tgctgcgcgg ccgatcgtct ggatgagtga cgtttccgac cgcaggaacc
      121 cttccttgtc ggcatcgagg atcgcgacga gggacacctc gggaaggtcg agaccctcgc
      181 ggagcaggtt gatccccacg aggacgtcga attccccgag ccggaggtcg cggatgatct
      241 cgatccgctg gatcgtgtcg acctccgagt ggaggtagcg gacgcggatc cccatctcga
      301 tcaggtagtc cgtgaggtct tccgccatct tcttggtgag ggtcgtcacc agcacccgct
      361 gctccgcctc ggctcggcgg cggatctcct cgatcaggtc gtcgacctgg cccttggtcg
      421 gccggatctc gacctccggg tcgatcaggc cggtgggccg gacgatctgt tccacgatcc
      481 ggtccgacac cttcagctcc caggggcccg gcgtggcgga catgtacacg atctggtgga
      541 cccgttcctt gaactcctcg aagcggagcg gacggttgtc gatcgcgctc ggcagccgga
      601 acccgaagtc gacgagcgtg gacttccggg agatgtcgcc ctcgtacatg ccgtggagct
      661 gtggcaccgt cacgtgcgac tcgtcgatca cgaccaggta gtcgtccggg aagtagtcga
      721 ggagcgtgta cggggcgctc ccggcagccc gcccgtcgat gtggcgcgag tagttctcga
      781 tccccgagca gaagccgacc tcgcggagca tctcgaggtc gtacgtcgtg cgcatccgga
      841 gtcgctccgc ctcgagtaac ttgctctggg actccagctc ggcgagtcgc tcgtggagct
      901 cttcctcgat cgtgacgagg gcgcgctcca tccgctcgtc gctcgcgacg tagtgcgatg
      961 ccgggaacgt ggtgaggtcc gcgatggtgt cggtgaggag ctctcccgtg agcgggtcga
     1021 accggctgat ccgttcgatc gtctcgcccc accactcgac ccggtatccg acctgctcgt
     1081 agctcgggaa gacctcgagg gtgtcgccct ggacgcggaa cgtgccgcgc gcgcggctca
     1141 cgttgttccg ctggtactgg atgtcgacca ggcgctgcat cgcgaactcc atcgggaggt
     1201 ccgttccgcg gaccgtgcgc aggagctgcc cctcgtactg ctccggcgat ccgaggccgt
     1261 agatgcagga gacgctggcc acgatcagga cgtcgcgccg agagagcagc gccgcggtgg
     1321 ccgagtgccg caggcgctcg atctcgtcgt tcactgacga gtccttctcg atgtaggtgt
     1381 cggtctgcgg gacgtaggcc tcgggctggt aatagtcgta gtagctcacg aagtactcga
     1441 cggcgttctc cgggaagacc tcgcggaact cgttcgccag ctgtgccgcg agcgacttgt
     1501 tcggagcgat cacgagcgtg gggcgttgga cccgttcgac caggtgggca acggtgaacg
     1561 tcttccccgt gcccgtcgcc cccagcaggg tgacgtgctc ctgacccgcc tgcacgccgt
     1621 ccacgagccg atcgatcgcc tccggctgat caccggatgg acggaactcg gacgtgatct
     1681 tgaagggcgg catcggccac gtcagtctag gaccgcccgg cgacagggcc tctcgggcaa
     1741 gaggcagcct acatccggcc ggcggcgatg atccgagcga ggaagcggcg ggtggcttcc
     1801 tcgcgcggct cggagaacat ccgctccggc ggcccctcct cgaggatccg tccctcctcg
     1861 aggaacgcca ctctgttcgc gacgtcgcgg gcgaagccca tctcatgggt cgcgatcacc
     1921 atcgtcatgc cgccggcggc gagctcccgg atcgcgtcca ggacctcggc cacgagcacc
     1981 gggtcgaggg cgctggtcac ctcgtcgagg agcaggagat ccgggcccat cgcgagcgct
     2041 cgcgcgatcg cgacgcgttg ctgctgaccc cccgagagac ggtcagggta ctcctccgcc
     2101 ttctcgatca ggccgaaccg cgagagcagc tcggtcgcct tggcgcgcgc gtcggcgggc
     2161 gtccgtccca gcgccttctc ctgcgccagg gtgacgttcc gcatcaccgt catgtgcggg
     2221 aacaggttga acgcctggaa cacgatcccg atccgccggc ggaccgcgtt ctggtcgacg
     2281 ccgccgcccg tgatctcgaa cccctcgacc cagatgcggc cggcgtcgac ctcctccagc
     2341 agatcgatgc accgcaggag cgtcgacttc ccggatccgc tcgcgccgat caggcagacg
     2401 acctcgtgat cttggaccac gaggtcgaca tcgcgcagca cctgcagctc gccgaaggac
     2461 ttccgaacct tgtccacgcg aaccgcctcg gtcacggcgt cccccgtgcc tgtcgccgcc
     2521 gccgatcccg tgcgatcaac caatcggtca gccgcgccaa ggggatcgtc atcaggatga
     2581 acaggaacgc cgcgaccacg aagctcgagt agttgaaacg gtaggcggag tagatctgcg
     2641 ccgcccgcgc cgcttccacg acgccgagga cggcgagcaa cgccgtgtcc ttctggaggc
     2701 tcacgaagtc gttgaggagc ggcgggatga tccggcggac ggcctgtggc agtacgacga
     2761 agcgcagcga ctgcatccgg gagaggccca gggatcgggc ggccgcgacc tggctgggat
     2821 gcaccgactc gatcccggcc cggtacacct ccgcgacgta cgccgagtag gagaggacca
     2881 gtgccgcggt cgcgtacacg acctccgatt gggtcgacag accgcggatg ccgagcgccg
     2941 gcaggccgaa cccgaacagg tagatcacca ggagcagggg catgccccgg aacaggtccg
     3001 tgtagacgat cgcgatcagg cggaacggga agaacaccgg tccctggagg ctccggatca
     3061 cggcgatcac gagggcgagg atcaggatca ggacctcggc gatcaggaag atccggatgt
     3121 tgagcaggaa cgcctgtccg atcccgggca ggccctcctc cggcacgccg aagaacgact
     3181 ccttcatcgc ctcgaggttg aagaagctct cgcggacgga atcgaacccg ggtgccgcaa
     3241 cgaggatcac cgtcaccacc gcgaagaaca cgatcgtgct ggctgtggag atcaggaagc
     3301 cccggttccc gagcaggcgg gacctccggt cgtgccccgt cagggcctcc ggcggctcgg
     3361 tcacagtgag ccccgggtgc cgactactgg atcacgggga cggccaggta gtcggccagc
     3421 cattcgctct ggaggtcctc cagggtgccg tcggccgtca tctcggcgag cgccaggttc
     3481 acgcactcca ccagaggact cccctcctcg aagaggatcc cgaggtactc gcccgcattc
     3541 gggaattggc ccaccagggc gccgttcggc gtgttgatcg tcgcctcgaa gtacgcggtg
     3601 ggcagatcca ggacgagccc gtcgacgctc ccgttccgga gcgcggcctt cgcgtcgctc
     3661 gtggagtcga acacccgggg atctaccgtg ggctggatca ccgtgttgat gaacgagagg
     3721 ctcgtcgtcc cgatctgggc cccgaagacg gagtccttga tctcctccat cgtcgtcgcg
     3781 ttctcgatcg gcgagccctt cagcaccatc agcgcctgcg tgaggtcgta gtagccgtcg
     3841 ctgaagtcga cggcctgatc ccgctcctcg gtgacggaga tgttgttgat gtcgaagtcg
     3901 aagtccttcg gcccgggagc gtaggaactg ttgaacggga cgttgaccca gttgacctgt
     3961 gagtccgcga agcccatccg ctccgccacc gcataggtca acgcggcctc g
//
LOCUS       DASUVE010000108         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_619407, whole genome shotgun sequence.
ACCESSION   DASUVE010000108 DASUVE010000000
VERSION     DASUVE010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619407"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VF036_02190"
     CDS             <1..710
                     /locus_tag="VF036_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX6264071.1"
                     /translation="SEHAALAMALRGERREDLKRVVLTGSGGPFRGWTATELARASMK
                     EALQHPVWSMGPKITIDSATLMNKGLEVIEAHYLFGLEYSQIHVLIHPEALVHAIAEF
                     RDGSMRAEMATADMRLPIQLALAWPERLPTGIASLPLTESPLTFEPVDHEAFPAVDLA
                     YRVGALGLTFPAVMNAANEVAVMAFLEGKIPLPRIVEIVQTIVDEHEPPSVVSVVHIE
                     RADIWARRRTAEIIEAR"
     gene            711..1835
                     /locus_tag="VF036_02195"
     CDS             711..1835
                     /locus_tag="VF036_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX6264072.1"
                     /translation="MGGPLAIVGFLALILLVVIPIHEFGHLLVARRFGFKVQEYFVGF
                     GTKLWSTKRGETEYGIKAIPAGGYVKIAGMNPYETVPAHDVARAYGSKPIRQRALVIL
                     AGPLSHVLVGGLLYFTLFVTYGDTNTELVVVGEVAPTLDGSASPALEAGLQVGDVVTR
                     VGDLEAPTPDSLGRYVTRYAEDHPGEPLAYVIERDGRSLTLEIVPRLVTEDEVTRGRI
                     GFTLGAEDLSIPGSVVESAEWVGRAAWNSVTTIPKVFTVGVGRTFSVLFTDEPRRDDD
                     VTSLVGVSRQIGGAGERGDWALFLNFAAYVTIFIGVVNLIPLPPLDGGHLMLLAWEKV
                     TGRQADYRRLVPVSAAVIVFLSVFAIATVFLDITEPLPLI"
BASE COUNT          314 a          682 c          714 g          363 t
ORIGIN      
        1 actccgagca cgcggcgctc gcgatggcgc tccgcggtga gcgccgggaa gacctcaagc
       61 gagtggtgct cacgggctcg ggcggcccgt tccgcgggtg gaccgctacc gagctggcga
      121 gggcatcgat gaaggaagcc ctccagcacc ccgtgtggtc gatggggccc aagatcacga
      181 tcgactccgc cacgctcatg aacaagggcc tcgaggtgat cgaagcgcat tacctcttcg
      241 gtctggagta ttcgcagatc cacgtgctca tccatcccga ggccctggtt cacgcgatcg
      301 cagagttccg ggacggatcg atgcgagccg agatggcaac ggctgacatg cggctgccga
      361 tccagctcgc cctggcctgg ccggagcggc tgccgacggg catcgcgtcg cttccgctca
      421 cggagtcgcc gctcacgttc gagcccgtcg atcacgaggc cttcccggcc gtggacctcg
      481 cctatcgggt cggcgcactg ggactcacgt tcccggcggt gatgaacgcc gcgaacgagg
      541 tggccgtgat ggccttcctc gaggggaaga tcccgctgcc tcgcatcgtg gagatcgttc
      601 agaccatcgt cgacgagcac gaaccaccct cggtcgtctc ggtcgtgcac atcgagcgcg
      661 cggacatctg ggcccgtcgc cgcacggcgg agatcatcga ggctcgctag gtgggcggcc
      721 cgctcgcgat cgtcgggttc ctcgccctga tcctgctcgt cgtgatcccg atccacgagt
      781 tcggccacct cttggtggcg agacgcttcg gattcaaggt ccaggagtac ttcgtcggct
      841 tcgggacgaa gctctggtcg acgaagcgtg gcgagacgga gtacggcatc aaggcgatcc
      901 ctgccggcgg ctacgtgaag atcgccggga tgaatccgta cgaaaccgtt cccgcccatg
      961 acgtcgcgcg cgcgtacggg tcgaagccga tccggcagcg cgcgctggtg atcctggccg
     1021 gcccgttgtc gcatgtgctc gtcggcgggc tcctgtactt caccttgttc gtcacgtacg
     1081 gggatacgaa caccgagctc gtggtggtgg gcgaggtcgc cccgacgctc gacggaagtg
     1141 cctcgccagc tctcgaagcg gggctccagg tcggcgatgt cgtgacccgc gtcggcgatc
     1201 tggaggctcc gacgcccgat tccctcggcc gctacgtcac ccggtacgcg gaggaccatc
     1261 ccggcgagcc gctcgcgtac gtgatcgaac gcgacggccg ttccctcacc ctcgagatcg
     1321 tcccccgtct cgtcacggag gacgaggtca cgaggggacg gatcgggttc acgctcggcg
     1381 ccgaggacct gtcgatcccc gggtcggtgg tggagtcggc ggagtgggtg gggagagccg
     1441 cgtggaacag cgtgacgacc atcccgaagg tcttcacggt gggcgtcggt cgcacgttct
     1501 cggtgctgtt caccgacgaa cctcgacgcg atgacgacgt cacgagcctg gtgggcgtca
     1561 gtcggcagat cggcggcgcc ggcgagcgtg gggactgggc attgttcctg aacttcgccg
     1621 cgtacgtcac gatcttcatc ggcgtggtga acctgatccc gctcccaccg ctggacggcg
     1681 gtcacctgat gctcctggcg tgggagaaag tgacgggccg gcaagccgat taccggaggc
     1741 tcgtccctgt gtcggcggcg gtcatcgtct tcctctcggt cttcgcgatc gcgacggtct
     1801 tcctcgacat cacggagccg ctgccgctca tctaggccgc gaacgctcac gtgtgaggat
     1861 cgagcgggcc tcaccacgat ttctccacgc cgcggaggcg ctgtgggcgg ggtggggtgg
     1921 atacgatgac gtcgtggctg ctcagaccgt gctcgtcgtc gaggacgagg acgcgatcgc
     1981 cgaagctgtt cgggcccgtc tccggagcga gggatatcac gtcgtcgtcg cctccgacgg
     2041 tcccgaggcc cttcgtcgac acgccgagac ccg
//
LOCUS       DASUVE010000109         2371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1373104, whole genome shotgun sequence.
ACCESSION   DASUVE010000109 DASUVE010000000
VERSION     DASUVE010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373104"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2015
                     /locus_tag="VF036_02200"
     CDS             <1..2015
                     /locus_tag="VF036_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX6264073.1"
                     /translation="DRRFYQHHGIDGRAILRASYVNLRDGSIEEGGSTITQQLVKNLY
                     TGGERTIARKLSEASLAWQLEDRLSKEEILTRYLNTVYFGQGAYGVQAAARTFFGVDA
                     IDLNLARSALLAGLITSPGHFDPYEYPSRANGRRRVVLRLMHEFGMITEEQRRHAGRA
                     PIHLHPVTTFGRYHYPYFIDYLKEWFLTNPAFGDTREDRYKLLFTGGLQIHTTLEPAV
                     QSAAQRAVDSVLSYPDDPSAAVTVMDPRNGFVLAMVGGDQRDYWRDRHAGRVNLATAM
                     GGTGRQSGSSFKPFALVAALEAGMSPAETFPAPSTLQLALETGEIWNVTNAEGAGYGT
                     MTLRSATIRSVNTVYAQLIDRLGPTAVVEVAERMGLRCCRRVSNPGGPLDPYLSAVLG
                     TNEVNTLEMATAYGTLATGGVRSRPVPVSRVTDARGTVLWSANTTGDRVLDPQIASVA
                     NEILNEAVLYGTGTAANIGRPQIGKTGTAMDHNDAWFVGAVPQMVAAVWVGFPQGQIR
                     MEPPRTRITVFGGTWPAQIWRLLMLEATAGLPEMTFPNPEVGFVSVSVDASQTPNCLP
                     NPFTLPQNIETLQVIEGTEPTEMCTSPSSLQEVLVPSTVGSSESIAIEALTQAGFYID
                     VKTAASTQPKGTVISQIPSAGTEAFQTSTVTITVSSTVPEGSAASP"
     gene            complement(1969..>2371)
                     /locus_tag="VF036_02205"
     CDS             complement(1969..>2371)
                     /locus_tag="VF036_02205"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX6264074.1"
                     /translation="TYGMHHCMNVVTGGTAEGSAVLLRAAEPLEGEEEMVVRRGRERP
                     VDLCSGPGRLTQALAVDRRDDGLDLVAGQGLWLEASSRAEAIAVGKRVGVHETSRSWR
                     YWLDGSPFVSRGRPGPPTAKRRIPRARSMTP"
BASE COUNT          391 a          845 c          773 g          362 t
ORIGIN      
        1 aggaccggcg cttctaccaa caccatggga tcgacggtcg agcgatcctc cgcgcgtcct
       61 acgtgaacct ccgagacggg agcatcgagg agggcggctc gacgatcacg cagcagctgg
      121 tcaagaacct ctacaccggg ggcgaacgca cgatcgcccg caagctgagc gaagcatcgc
      181 tcgcctggca gctcgaggat cgcctgtcca aggaggagat cctcacgcgg tacctgaaca
      241 cggtgtactt cgggcagggt gcctacggtg tccaggctgc ggcccggacg ttcttcggcg
      301 tcgacgccat cgacctcaac ctggcccgct ccgccctgct cgccggactg atcacgagcc
      361 ccgggcactt cgatccgtac gagtacccga gccgggcgaa cggacgccga cgcgtggtgc
      421 tccgcctgat gcacgagttc gggatgatca cggaggagca acgtcgtcac gccggcagag
      481 cgccgatcca cctccacccg gtgacgacct tcggccgcta ccactacccg tacttcatcg
      541 actacctgaa ggagtggttc ctgacgaacc ccgccttcgg cgatacgcgg gaggaccggt
      601 acaagctcct gttcaccggc ggcctccaga tccatacgac cctggagccg gccgtccagt
      661 ccgccgccca gcgcgcggtc gattcggtac tctcgtatcc ggacgacccc tccgccgccg
      721 tgaccgtgat ggatccgcgc aacggcttcg tgctggcgat ggtcggcggc gaccaacgcg
      781 actactggcg ggatcgtcac gcgggacgcg tgaacctggc gaccgcgatg ggcgggacgg
      841 ggcgacagtc cggctcctcg ttcaaaccgt tcgcgctggt ggcggccctc gaggccggca
      901 tgtctccagc ggagacgttc cccgcaccct cgacgctcca gttggccctc gagaccggcg
      961 agatctggaa cgtcacgaac gccgagggtg cgggatacgg aacgatgacg ctccgctcgg
     1021 cgacgatccg gtcggtgaac accgtctacg cccagctgat cgaccgactc ggtcccaccg
     1081 ccgtcgtgga ggtggccgag cggatggggc tccgctgctg ccgtcgcgtg agcaaccccg
     1141 gcggtccgtt ggatccctac ctctccgcgg tcctgggaac gaacgaggtc aacacgctcg
     1201 agatggcgac cgcgtacggc accctggcga cgggcggcgt ccggtcccgg cccgtcccag
     1261 tgtcccgtgt caccgacgct cgcggcaccg tgctgtggtc cgccaacacg accggcgatc
     1321 gcgtcctcga tccgcagatc gcgtcggtgg cgaacgagat cctgaacgag gccgttctct
     1381 acggcacggg gaccgcggcg aacatcggca ggccgcagat cggcaagacg ggcaccgcga
     1441 tggaccacaa cgacgcctgg ttcgtgggcg ccgtcccgca gatggtcgca gcggtgtggg
     1501 tcgggttccc tcagggacag atccggatgg aaccgccgag gacgaggatc accgtcttcg
     1561 gaggcacgtg gccagcgcag atctggcggc tgctgatgtt ggaggcgacc gccggactgc
     1621 cggagatgac gttcccgaac cccgaggtgg ggttcgtctc cgtctccgtg gatgcctcgc
     1681 agacgcccaa ctgcctgccg aacccgttca cgctcccgca gaacatcgag accctgcagg
     1741 tcatcgaggg aacggagccc acggagatgt gcacgtcacc gtcgtcgctc caggaggtgc
     1801 tcgttccgtc cacggtcggt tcctcggagt cgatcgcgat cgaggcgctg acccaggccg
     1861 gcttctacat cgatgtgaag acggcggcct cgacgcagcc gaagggcacg gtgatctccc
     1921 agatcccctc ggcgggcacc gaggccttcc agaccagcac cgtgacgatc acggtgtcat
     1981 cgaccgtgcc cgagggatcc gccgcttcgc cgtaggcggt ccgggtcgtc ctcgagacac
     2041 gaacggactc ccgtcgagcc aatagcgcca gctccgactc gtctcgtgca caccgacgcg
     2101 cttccccacg gcgatcgcct ccgctcgcga ggatgcctcg agccacaggc cctggcccgc
     2161 gaccaggtcg agcccgtcgt cgcgtcgatc gaccgcgagc gcctgggtga gccgaccggg
     2221 tcccgagcac aggtcgacgg gtcgttctcg ccctcgccga acgaccatct cctcctcgcc
     2281 ctcgaggggc tcggcggcac gcaggagcac cgcgcttccc tccgcggtcc ctccggtcac
     2341 gacgttcatg cagtgatgca tcccgtaggt g
//
LOCUS       DASUVE010000110         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_54668, whole genome shotgun sequence.
ACCESSION   DASUVE010000110 DASUVE010000000
VERSION     DASUVE010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54668"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VF036_02210"
     CDS             complement(<1..292)
                     /locus_tag="VF036_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX6264075.1"
                     /translation="MGQKVHPYGFRLGVIYPWKSNWYANRRDYIEALHEDVWIRKHIR
                     SRLSRAGISSIDIERKGDQIWVYIRTARPGIVIGRKGAEVDRLRKDVERYTKK"
     gene            complement(292..636)
                     /gene="rplV"
                     /locus_tag="VF036_02215"
     CDS             complement(292..636)
                     /gene="rplV"
                     /locus_tag="VF036_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX6264076.1"
                     /translation="MEAKATVKYVRTSPRKMRRTVDLIRGQHVGEAKRILRLSTLGAS
                     RDVEKLLDSAIANAEQRPGVIAENLLVSKAWVDEGPTLRRFRPRAYGRATRVRKRTSH
                     VTVVVETMGDEE"
     gene            complement(637..918)
                     /gene="rpsS"
                     /locus_tag="VF036_02220"
     CDS             complement(637..918)
                     /gene="rpsS"
                     /locus_tag="VF036_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX6264077.1"
                     /translation="MARSVKKGPFVDEHLMVKIAGMNERSEKKVVKTWSRRSTIVPDM
                     IGHTIAVHDGRKHVPVFISESMVGHKLGEFAPTRTFRTHSRDERRTGPR"
     gene            complement(918..1355)
                     /gene="rplB"
                     /locus_tag="VF036_02225"
                     /pseudo
     CDS             complement(918..1355)
                     /gene="rplB"
                     /locus_tag="VF036_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440389.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
     gene            1359..1856
                     /gene="rplB"
                     /locus_tag="VF036_02230"
                     /pseudo
     CDS             1359..1856
                     /gene="rplB"
                     /locus_tag="VF036_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
     gene            1856..2137
                     /gene="rpsS"
                     /locus_tag="VF036_02235"
     CDS             1856..2137
                     /gene="rpsS"
                     /locus_tag="VF036_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX6264078.1"
                     /translation="MPRSLKKGPFVDEHLAKKVDDMNKKGDKRVIKTWSRRSTIIPDM
                     LGHTIAVHDGRKHVPVFISEAMVGHKLGEFAPTRTFRSHARDERRTGVR"
     gene            2138..2485
                     /gene="rplV"
                     /locus_tag="VF036_02240"
     CDS             2138..2485
                     /gene="rplV"
                     /locus_tag="VF036_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020109929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX6264079.1"
                     /translation="MAEARATAKYLRTSPRKMRRAIEMIRGQHVEEARRILRFSPLGA
                     THDVGKLLNSAIANAEQQPGVIAENLVVDRAWVDEGPTLRRYRPRAYGRATRVRKRTA
                     HVTVVVKTMGDEG"
     gene            2485..>2844
                     /gene="rpsC"
                     /locus_tag="VF036_02245"
     CDS             2485..>2844
                     /gene="rpsC"
                     /locus_tag="VF036_02245"
                     /inference="COORDINATES: protein motif:HMM:NF019275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX6264080.1"
                     /translation="MGQKVHPYGFRLGVIYPWKSNWYADRDYAQHLHEDIWIRKHIRS
                     RLSRAGISSIDIERKGDQIWVFIRTARPGIVIGRKGAEVDRLRKDIERFTKKRVDVKV
                     EDMNQAASESRPETDATL"
BASE COUNT          452 a          994 c          951 g          447 t
ORIGIN      
        1 gcttcttcgt gtagcgctcg acgtccttgc gcaagcggtc cacctcggcg cccttgcggc
       61 cgatgacgat gccggggcgg gcagtccgga tatagaccca gatctgatcg cccttgcgtt
      121 cgatatcgat cgaggagatc ccggcgcgcg agagccgcga gcggatgtgc ttgcggatcc
      181 acacgtcctc gtggagcgcc tcgatgtagt cgcgccggtt cgcgtaccag ttcgatttcc
      241 aggggtagat gacccccagc cggaacccgt atgggtggac cttctgtccc actactcctc
      301 gtctcccatc gtctccacca cgaccgtcac gtgcgacgtg cgcttccgga cccgggtggc
      361 ccggccgtag gcccggggac ggaaccgacg gagcgtcggc ccctcgtcca cccatgcctt
      421 cgacaccaac aggttctccg cgatcacgcc aggacgctgc tcggcgttgg cgatcgcgga
      481 atcgagcaac ttctccacgt cccgcgatgc ccccagcgtc gacagccgga ggatgcgctt
      541 cgcctcaccc acgtgctggc cgcggatgag atccaccgtc cgccgcatct tgcgcggcga
      601 cgtgcggacg tacttgaccg tggctttcgc ttccatctat cgaggacccg tccttcgctc
      661 atccctcgag tgcgtccgga acgtgcgcgt cggagcgaac tcgccgagct tgtgcccgac
      721 catcgactcc gagatgaaca cggggacgtg tttccggcca tcgtggaccg cgatcgtgtg
      781 tccgatcatg tccgggacga tcgtggaacg gcgcgaccag gtcttcacga ccttcttctc
      841 gctccgctcg ttcatccccg cgatcttcac catgaggtgc tcgtccacga acggcccctt
      901 cttcacggac ctcgccatca gccacgcccc ttcccgcgac gccggatgat ctgcgcgttc
      961 gacgccttct tcttcttccg cgtacggcct tccttcttgc cccacggcga cgtcggatgg
     1021 cgtccaccgg aggctttccc ttcgccgccg ccgagcgggt ggtccaccgg gttcatggcg
     1081 acgccccgca ccgagggccg cttgcccttc cagcggttcc gccccgcctt gccgatcgcg
     1141 atcagctcgt gctcggcgtt cccgaccacc ccgacggtgc ccttgcagat ggacgggacc
     1201 gtccgcatct cgcccgaggg aaggcgcagc gtggccagct tcccctcctt cgccatcagc
     1261 tggacgctcg tccccgccga ccgcgctatc cgggctccgg cgccgggctt cagctcgacc
     1321 gcgtgcacga cggtgccgac cgggatgttc cgcagcggca gggcgaccgg ctgatgagcg
     1381 gtcccgacgc cgagatccgt cccggcaacg cgctcccgct ccggaacatc cctgtcggca
     1441 ccgtggtcca cgcgatcgag ctgaagcccg gcgcgggcgc gaagatggcc cgctcggcgg
     1501 gtgcgagcgt gcagctgatg gccaaggagg gcgcgaaggc gaccctgcgc ctgccgtcgg
     1561 gcgagatgcg gatggtgccc gccagctgca aggcgaccgt cggctcggtc ggcaaccccg
     1621 agcacgagct gatctcgatc ggcaaggccg gccgggcccg ctggaagcgc aagcgcccgt
     1681 cggtgcgcgg cgtcgcgatg aacccggtcg accacccgtt gggcggcggc gagggcaagt
     1741 cctcgggcgg ccgtcacccc acctccccgt gggggcagaa ggaaggtcgg acgcgcaaga
     1801 agaacaaggc gtcgaacaag gatatcgtcc ggcgtcgcgg gaaggggcgt ggctgatgcc
     1861 tcggtcgctg aagaagggtc cgttcgtcga cgagcatctc gcgaagaagg tcgacgacat
     1921 gaacaagaag ggcgacaagc gcgtgatcaa gacctggtcg cggcgctcca cgatcatccc
     1981 cgacatgctc gggcacacga tcgcggtcca cgacggtcgc aagcacgtgc cggtgttcat
     2041 ctccgaggcg atggtcggcc acaagctcgg cgagttcgct ccgacgagga cgttccgctc
     2101 gcacgcgcgg gacgagcgca ggacgggggt ccgctgagtg gccgaagcgc gcgcgaccgc
     2161 gaagtacctg cgcacgtcgc ctcgcaagat gcgccgcgcg atcgagatga tccgtggtca
     2221 gcacgtcgag gaggcgcggc ggatcctgcg gttctcgccg ctcggggcca cgcacgacgt
     2281 gggcaagctg ttgaactccg cgatcgccaa cgccgagcag cagcccggcg tgatcgccga
     2341 gaacctggtc gtcgaccggg cgtgggtcga cgaggggccg acgctccggc ggtaccggcc
     2401 gcgcgcgtac gggcgggcga cgcgggtgcg caagcgcacc gcgcacgtga cggtcgtggt
     2461 gaagacgatg ggagacgagg gctagtgggt cagaaggtcc atccgtacgg gttccggttg
     2521 ggggtcatct acccctggaa gtccaactgg tacgccgatc gcgactacgc acagcacctc
     2581 cacgaggaca tctggatccg caagcacatc cgttcgcggc tgtcccgcgc cggcatcagc
     2641 tcgatcgaca tcgagcgcaa gggcgaccag atctgggtgt tcatccgcac ggcccggccc
     2701 ggcatcgtga tcggccgcaa gggcgccgag gtcgaccgtc tgcgcaagga catcgagcgc
     2761 ttcacgaaga agcgggtcga cgtgaaggtc gaggacatga accaggccgc gagtgagtcg
     2821 cgcccggaga ccgacgccac gctg
//
LOCUS       DASUVE010000111         3114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1185031, whole genome shotgun sequence.
ACCESSION   DASUVE010000111 DASUVE010000000
VERSION     DASUVE010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185031"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1264
                     /locus_tag="VF036_02250"
     CDS             44..1264
                     /locus_tag="VF036_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264081.1"
                     /translation="MPDLEELFHSLERTPAPDLWEDIAEREPRRTLGPSGGRRVVVVV
                     VALLIGASSVGLAVWALRPTDDRSRPAADPMTTIAFTSGVGGYHLAAVTLDGAVTHLT
                     HPSGGAYDLGPTWSPSGDAIAFLRYTTGPGEDGGDYELVVADAGGRLVTHFDLTAVSF
                     SWSPDGSRIAISSFEEGTDHDIVVAARDGTGVRVIVDSPRSDTSPAWSPEGDLIAFTS
                     RPVLDRDPGDEHIYVVRSDGTDLTKLTGPGPHGSPAWSPNGARIAYVGARDGESEIFV
                     MQADGSGRLALTDAPTNDVTDPVWSPDGSRIAFGVYSGTDWDVYVVDVDGTDQVPVAD
                     GPKDEVGPAWAPDGGLIAYSAAESAESCRCDNAARFDIFLVEPNGDGRRRLTIDASEL
                     GGGLAWRPPARGTD"
     gene            1374..2144
                     /locus_tag="VF036_02255"
     CDS             1374..2144
                     /locus_tag="VF036_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX6264082.1"
                     /translation="MNVVVLTKWVPEPEGTPTLGENGLLVREGADGSLDPGDEYGLEA
                     ALQLVEAAGDGEITLLTMGPEIAQAALQRGLAMGAHQGVRITDESLRGADSLVTARVL
                     AAAIKRKPFDLVVGGVESTDGYTGIVPAAIAELLGLPSLTFARELHLSDDTLTIERQT
                     AAGYDVVVAPLPLVITVTAGATEPRYPSLKGVMAAKSKPVETLTVADLGLSSDDVTAT
                     QTVANVDDAPPKQGGETVEFDETTAARIADLLAEAKVI"
     gene            2146..3108
                     /locus_tag="VF036_02260"
     CDS             2146..3108
                     /locus_tag="VF036_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX6264083.1"
                     /translation="MKIWVWADVTQDGPAPSVLELLTKARELGEDVAAVALGPGATAS
                     AGALGEHGATKLFASDDEVFANHLSQPAVHALAGLVEEHSPDLILFPASYDARDVAGR
                     LHARIGSTLMANATDVLASDEARTEILGGTKVVDVELKGPAPRLVIVRPQSFASEPSG
                     GTAEVVEVKVDVPDELTSAERTERHEEAASGPRLESAKVVVAGGRGLGGPDAFGQLET
                     LAAAIGNAAVGASRAAVDAGWVPYSFQIGQTGKTVKPEVYICAGISGALQHLVGMKAA
                     KRIVAITKDPDAPILKIADLGVVGDLFQVVPALTEEIEKRRTGS"
BASE COUNT          482 a         1077 c         1105 g          450 t
ORIGIN      
        1 atgcgcggac ggagacggct ccgtgagctg ctggagggga acgatgcctg atctcgaaga
       61 actcttccac tcgctcgagc gcacgccggc accggatctg tgggaagaca tcgctgagcg
      121 ggaacctcgt cggacgctcg gtccgtccgg cggccggcgc gtcgtggtgg tcgtcgtggc
      181 gctcctcatc ggcgcatcgt cggtcggtct cgccgtttgg gcgcttcggc cgacggacga
      241 ccggtcccgc ccagcggcgg atccgatgac cacgatcgcg ttcacgagcg gggtgggcgg
      301 ctatcacctc gccgcggtga cgctcgacgg cgccgtcacc catctgacgc accccagcgg
      361 tggggcatac gacctcggac cgacctggtc tccgagtgga gacgcgatcg cgttcctccg
      421 atacacgacg ggccctgggg aggacggtgg tgactacgaa ctggtcgtcg ccgacgcggg
      481 cggtcgtctc gttacccact tcgatctgac cgccgtcagc ttctcgtggt cgcccgacgg
      541 ttcgcgcatc gcgatctcga gcttcgagga gggcaccgac cacgacatcg tcgtcgcagc
      601 tcgggacgga accggcgtcc gagtcatcgt cgactcgccg cgctcggaca cgagcccggc
      661 gtggtctccg gagggagatc tgatcgcatt caccagcaga ccggtcctcg atcgagatcc
      721 gggcgacgaa catatctacg tcgtccgctc cgacggcacg gatctgacca agctcacggg
      781 ccccgggccg cacgggtctc ccgcctggtc tccgaacgga gccaggatcg cgtacgtcgg
      841 cgcgcgggat ggcgagtccg agatcttcgt gatgcaagcc gatggctccg gacgcctcgc
      901 cctgacggac gcccccacga acgacgtcac cgatcccgtg tggtcgccgg acggatcacg
      961 gatcgcgttc ggggtgtact cgggcacgga ctgggacgtg tacgtggtcg acgtcgacgg
     1021 gacggatcag gtgccggtgg ctgatgggcc caaggacgag gtcggcccgg cctgggcacc
     1081 cgacggcggg ttgatcgcgt attcggcggc cgagtccgcc gagtcgtgcc ggtgcgacaa
     1141 cgctgcacgc ttcgacatct tcctggtgga gccgaacggc gacggccgca gacgcctcac
     1201 gatcgatgcg agcgagctcg ggggtggcct cgcgtggagg ccgcccgccc gcggcaccga
     1261 ttgagcccag gttctcgcgc ccggttcggc cgaacgccgg accccgcctc gcgcctcgtg
     1321 ccgggcaacg cgccttccga gtacggtgct tccgtgagcg caatgagaca gccgtgaacg
     1381 ttgtcgtcct gacgaagtgg gtgcccgagc ccgagggcac gccaacgctc ggcgagaacg
     1441 gactcctcgt ccgcgaaggc gccgacgggt cgctcgatcc cggagacgag tacggcctcg
     1501 aggccgccct gcaactggtg gaagctgcgg gggacggcga gatcaccctc ctcacgatgg
     1561 gccctgagat cgcgcaagcg gcccttcagc gcggcctcgc gatgggcgcg caccagggcg
     1621 tgcggatcac cgacgaatcc ctccgcggcg ccgactcgct ggtcacggcc cgcgtgctcg
     1681 cggcagcgat caagcgaaag cccttcgatc tcgtggtggg cggcgtcgag tcgacggacg
     1741 ggtacacggg catcgtcccc gcggcgatcg ccgagctcct cgggctcccc agcctcacgt
     1801 tcgctcgaga gctgcacctc tcggacgaca ccctgacgat cgagcgacag accgcggcgg
     1861 gctacgacgt cgtggtagcg ccgctgcccc tggtcatcac cgtgaccgcg ggggcgaccg
     1921 aacctcggta cccgtcgctg aagggcgtga tggcagcgaa gtcgaaaccg gtcgagacgc
     1981 tgaccgtcgc ggatctcggg ttgtcgagcg atgacgtcac cgcgacgcag acggtggcca
     2041 acgttgatga cgctccgccg aagcagggag gcgagacggt cgagttcgac gagaccaccg
     2101 ccgcccggat cgcagacctc ctcgccgagg cgaaggtgat ctgacatgaa gatctgggtc
     2161 tgggccgacg tcacgcagga tgggccggcg ccgtccgtgc tcgagctcct cacgaaggcg
     2221 cgcgagctcg gcgaggacgt tgcagctgtg gccctcggtc ccggcgcaac ggcgtccgcc
     2281 ggcgccctcg gcgagcacgg cgccacgaag ctcttcgcct ccgacgacga ggtcttcgcg
     2341 aaccacctct cgcagccggc tgtccacgcg ctcgccggcc tcgtcgagga gcactccccg
     2401 gacctgatcc tgttcccggc ctcctacgac gcccgcgacg tcgcagggcg cctgcacgcc
     2461 cgcatcggct cgaccctgat ggcgaacgcc accgacgtgc tcgcctcgga cgaagcccgc
     2521 acggagatcc tcggcggcac gaaggtcgtc gacgtcgagc tgaaggggcc ggccccgagg
     2581 ctcgtgatcg tccggccaca gtccttcgcg tcggagccgt ccggcggcac cgccgaggtg
     2641 gtggaggtga aggtggacgt cccggacgag ctcacgtcgg ccgaacggac cgaacggcac
     2701 gaggaggccg cgagcgggcc gcgcctcgag agcgcgaagg tcgtcgtcgc cggcgggcga
     2761 gggctcggcg gtcccgatgc cttcgggcaa ctcgagaccc tcgcggccgc gatcgggaac
     2821 gccgccgtcg gtgcctcgcg cgcggcggtc gacgccggat gggtgccgta cagcttccag
     2881 atagggcaga ccggcaagac cgtgaagccc gaggtctaca tctgcgccgg gatcagtggc
     2941 gcgcttcagc acctggtggg catgaaggct gccaagcgga tcgtggcgat cacgaaggac
     3001 cccgacgcgc cgatcctgaa gatcgcggac ctgggcgtgg tgggtgacct gttccaggtc
     3061 gtcccggccc tcaccgagga gatcgagaag cgacggacgg gctcgtagcc gccg
//
LOCUS       DASUVE010000112         7092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_435717, whole genome shotgun sequence.
ACCESSION   DASUVE010000112 DASUVE010000000
VERSION     DASUVE010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435717"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VF036_02265"
     CDS             complement(<1..268)
                     /locus_tag="VF036_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264084.1"
                     /translation="MIAVGIWLLSVGLADLVAGLSGEPKDGARTAGAIVTGVVAPAIL
                     CEIADADVGTLVMLTLLTGATTAVWIVIRRDAPRMSAVRALAALL"
     gene            complement(265..927)
                     /locus_tag="VF036_02270"
     CDS             complement(265..927)
                     /locus_tag="VF036_02270"
                     /inference="COORDINATES: protein motif:HMM:NF027589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEX6264085.1"
                     /translation="MGSVGAERLVAVLGDVVASRRHPDRERRLTAVGSALDMANGRVG
                     AIQPLTPTIGDEFQGLYGDLVSALDATLVVRLALLGEVDVRFGIGSGELLGYEASRAP
                     FEQDGPAWWAAREAVDMVRDLEQRRERPRGLRTRWIDEGADGSPPVGAVNAFLHCRDE
                     LVTAMDPRDADIMLGLLVHEPLASIAERLDVSVSAVSQRAIRGGLYAIREAHEELREA
                     VA"
     gene            complement(1023..1481)
                     /locus_tag="VF036_02275"
     CDS             complement(1023..1481)
                     /locus_tag="VF036_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020199788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX6264086.1"
                     /translation="MEYIQAVKDDEGEGCVLCRIRDGEEPERVLARSEIGYVVLNKYP
                     FNPGHVMVVPNRHDGSFEALGDDELLDLQRLIRRSVSALRKEMAPHGFNVGANIGRIA
                     GAGMPEHLHWHVVPRWSGDTNFMPVVGQTRVMPELLEETAARLTPRFMDG"
     gene            complement(1515..3452)
                     /gene="thrS"
                     /locus_tag="VF036_02280"
     CDS             complement(1515..3452)
                     /gene="thrS"
                     /locus_tag="VF036_02280"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX6264087.1"
                     /translation="MALVPRLSLPDGTHVELPEGEPVGAVLAPDALAARVDGTLRDLS
                     FVPAGDASVEPVAPGSDDALHILRHSTAHVLAQAVCRLQPGARYAIGPPIQDGFYYDF
                     DLPTAIGEGDLPAIEAEMRAIVEDDQPFLREEVSREDAIARLEGQPFKREIIESIGSA
                     EGEVATGDVVSLYRNDGWVDLCEGPHVPSTARLGAFTLTSTAGAYWRGLETNPMLTRI
                     YGTAWATREDLEAHLSRLEQAEKRDHRRLGRELDLFSSPDELGPGLWIWHPRGGIFRK
                     QLEDWVRDLHVERGYDLIVTPHIARSVLWETSGHLEKYRENMYPPMEADTGDYYVKPM
                     NCPFHVLVYKSQVRSYRDLPMRLSELGTIYRHEKAGTLHGLLRIRGGTQDDSHIICTP
                     EQLIDEILAVFDLTLEIHRTFGFTDPVVELSTLPTAQSIVEAETAERATEALTLALEK
                     SGLEYRVAEGEGAFYGPKVDFHFRDALGRLWQLTTVQCDFALPERFDMEYTGDDNQRH
                     RPVMIHRAILGTLERFSAVLIEHYGGAFPLWLAPEQLRLVPVADRHVPNCMELAATAR
                     ASGLRVDVDETKETVGKKIRAAQLMKSPYVVVVGDRDLDAGTFTVRDRSGEEHQGVPF
                     DRIVSKLAEEARTRALEQSAF"
     gene            complement(3486..6032)
                     /locus_tag="VF036_02285"
     CDS             complement(3486..6032)
                     /locus_tag="VF036_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6264088.1"
                     /translation="MWRAALKSVLARKLRLVLTALAIVLGVGFVSGTLVLTDTALSAF
                     DDLFGDIFAKTDVVVQAENAFQDIAAGGGGGGGQERNPIPEEVLGDVQALPGVAAADG
                     SVGGFAQVIDPQTDEVISSGGAPTIGSSWDPDVTPFELTGEPPQAENEVVIDADTVDE
                     AGLEIGDDVRIVTPLGVYPYRLTGVISLPSATTIGGATVASFELETAQRLFDREGTYD
                     QIYIVAGEGASAASVAAQVQEVRPSGFQAITATDAAAEQTDQVREGLGFLRTALLVFA
                     FVALFVGAFVIFNTFNIVVTQRTREMGLLRALGASRGQVFTSVIIESLVIGLLGSALG
                     LAAGIGLAVLLKQGLELIGLELPPTPLAIRPTTIIAALVLGTVVTVVASVFPARRASR
                     VAPIEALREGTVAPTASLRRRAIVGGLLTTAGVGAIAAGLFGDVPQPAAIVGLGAQFT
                     FIGIAVLSPFVARPLATAIGGPARRLGMTGRLGQENAKRNPRRTASTAAALMIGLGLV
                     VFVSVFMTTLKASASDTLDETLRADFILSSSGFQPFSPGIADALSQRDEFAAVSPFRQ
                     AGVKIGGGLTFISGVDETTVDDVVSVPMEAGTTAALIEPGTILIYKGPAQARDLGIGD
                     ELPVAFARTGTKQLRIVGIYTDNRLLGDYTVSLEEYQANVAEQLDYLVFVRRADGVSL
                     EQARAALEEVAGEFPNVEINDQAQFKEEQDRLIDQVFGIVLVLLVLSVIISAFGIANT
                     LALSIYERVRELGLLRAVGMQRTQIGWMIVIEAVIVSLLGAVLGIAIGLLFGWAMQQA
                     LADLGVSRLAVPWGLLVVFAVIAGLLGLFASIAPAIRASRIKVLEAISYE"
     gene            complement(6062..6802)
                     /locus_tag="VF036_02290"
     CDS             complement(6062..6802)
                     /locus_tag="VF036_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003936171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6264089.1"
                     /translation="MLTETRTAAARAVDLRKVYGEGTAAVEALRGVSVEFAAGEYTAI
                     MGASGSGKSTLLHCMAGLDKPTSGTVWIGDQDLTHLKDHDLTEIRRDRVGFVFQAFNL
                     VPTLTAAENITLPTAIAGRDVDRARFDEVVDTIGLRDRLTHLPSELSGGQQQRVAAAR
                     ALVSRPEIVFADEPTGNLDSRASQELLTFLKAAVTERGQTIVVVTHDPVGASYANRIV
                     FLADGQVVDEMRDPTAERVLDRMKLLEA"
     gene            complement(6881..>7092)
                     /locus_tag="VF036_02295"
     CDS             complement(6881..>7092)
                     /locus_tag="VF036_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6264090.1"
                     /translation="RSTRVLDRAHDHRHRSLRACRRVCDQGPGFDPAEIDWRGHRGGL
                     FLLDTLAYDWGIERNAAGTEVWFEI"
BASE COUNT         1160 a         2520 c         2337 g         1075 t
ORIGIN      
        1 acagcagcgc ggcgagtgca cggaccgcgc tcatgcgggg cgcgtcccgg cggatgacga
       61 tccacacggc cgtggtggcg ccggtcagga gggtcagcat gacgagcgtg ccgacatccg
      121 cgtcggcgat ctcgcacagg atcgccggcg ctacgacgcc ggtcacgatc gctccggccg
      181 tcctcgcacc gtccttcggc tcgcccgaga gtccggcgac gaggtccgcc agacccaccg
      241 acagcaacca gataccgacc gcgatcacgc gacggcctcc cgcagttcct cgtgcgcctc
      301 tcggatcgcg tacagaccac cgcggatcgc gcgctgggag acggccgaga cgctcacgtc
      361 caagcgctcc gcgatcgagg ccagcggctc gtggacgagc aatccgagca tgatgtcggc
      421 gtctcgcgga tccatcgccg tgaccagctc gtcccggcag tgcaggaacg cattgacggc
      481 tccgaccggt ggcgacccgt cggccccctc gtcgatccaa cgggtccgca gcccgcgcgg
      541 ccgttcccgc cgctgctcca gatcacgaac catgtcgacc gcctcccgcg ccgcccacca
      601 agccggaccg tcttgctcga acggggcccg cgacgcctcg taccccagca gctcgccgga
      661 cccgatcccg aaccggacat cgacttcacc gaggagggcg agccgcacga caagcgtcgc
      721 atcgagcgcc gacacgaggt ctccgtacag gccctggaac tcatcgccga tcgtcggcgt
      781 gagcggctgt atcgccccga ccctgccgtt cgccatgtcc aacgcgcttc cgaccgccgt
      841 caaccgtcgc tcgcgatccg gatgacggcg cgaagcgacg acgtcgccga gcaccgcgac
      901 gagccgttcg gctccaaccg atcccatggt gtgaagtgta tgtcttcagc tactgaaagt
      961 aaagatagac gcttcatatc gtctgagtga agatatgagc tgcagagatg cgataccggg
     1021 actcaaccat ccatgaaccg cggggtcaat cgagccgcgg tctcctccag cagttccggc
     1081 atcacccgcg tctgccccac gaccggcatg aagttcgtgt ctccgctcca tcgagggacc
     1141 acgtgccaat ggagatgttc cggcatcccc gccccggcga tgcgaccgat gttcgcgccg
     1201 acgttgaagc cgtggggggc catctccttg cggagcgccg agacggacct ccggatgagg
     1261 cgttgcagat cgagcagctc gtcgtcgccg agcgcttcga agctgccatc gtgacggttc
     1321 ggcacgacca tcacgtgacc cgggttgaag gggtacttgt tgaggacgac atagccgatc
     1381 tccgaccgcg cgaggacgcg ctcgggctcc tccccgtcgc ggatccgaca gagcacgcac
     1441 ccttcgccct cgtcgtcctt gacggcctgg atgtactcca tgcgccatgg actccacagg
     1501 tgccccataa cggtctagaa ggcgctctgt tcgagcgcac gcgtccgggc ttcctcggcg
     1561 agcttcgaca cgatccgatc gaacggcacg ccctgatgct cctcccccga tcggtcccgg
     1621 acggtgaacg tccccgcatc cagatcccga tcgcccacga ccaccacgta cggcgacttc
     1681 atcagctggg ccgcgcggat cttcttgccc acggtttcct tcgtctcgtc gacgtccacc
     1741 cggaggcctg atgctcgcgc cgtggcggcg agctccatgc agttgggcac gtgccggtcg
     1801 gccaccggga ccaggcggag ctgctccggc gcgagccaca gcgggaacgc gccgccgtag
     1861 tgctcgatca gaaccgcgct gaaccgctcc agcgtcccga ggatcgcccg gtggatcatc
     1921 accggacggt gccgctggtt gtcgtcgccc gtgtactcca tgtcgaagcg ctcggggagg
     1981 gcgaagtcgc actggacggt cgtgagctgc cacaagcgcc cgagcgcgtc gcggaagtgg
     2041 aagtccacct tcggtccgta gaacgcgccc tcgccctcgg cgacgcggta ctcgagcccg
     2101 ctcttctcga gcgccagcgt cagtgcctcg gtggcgcgtt cggccgtctc ggcctccacg
     2161 atcgactgag cggtcggcag cgtcgagagc tcgaccacgg ggtcggtgaa gccgaaggtc
     2221 cggtggatct cgagcgtgag gtcgaagacc gcgaggatct cgtcgatgag ctgctcgggc
     2281 gtgcagatga tgtgcgagtc gtcctgggtc cccccgcgga tccggagcag cccgtggagc
     2341 gtgccggcct tctcgtggcg atagatcgtg ccgagctccg acagtcgcat cggaaggtcg
     2401 cggtagctcc gcacctgtga cttgtagacg agcacgtgga acgggcagtt catcggcttg
     2461 acgtagtagt cgccggtgtc cgcctccatg gggggataca tgttctcccg gtacttctcg
     2521 agatggccgc tcgtctccca cagcacggac cgcgcgatgt gcggcgtgac gatgaggtcg
     2581 tacccgcgct cgacgtggag gtcccggacc cagtcctcga gctgcttccg gaagatgcca
     2641 ccgcggggat gccagatcca caggcccgga ccgagctcgt cggggctcga gaacagatcc
     2701 agctcgcgac cgaggcgtcg gtgatcgcgc ttctcggctt gctccagccg ggacaggtgc
     2761 gcctcgaggt cctcccgggt cgcccaggcc gtcccgtaga tccgggtgag catcgggttg
     2821 gtctcgagcc ctcgccagta ggcgccggcg gtcgaggtca gcgtgaacgc gccgaggcgg
     2881 gcggtcgagg ggacgtgggg gccttcgcac aggtccaccc acccgtcgtt gcggtacagc
     2941 gacacgacgt cgcccgtcgc cacctcgcct tcggccgaac cgatcgactc gatgatctca
     3001 cgcttgaacg gctggccctc gagtcgggcg atcgcgtcct cgcgcgagac ctcttcgcgg
     3061 aggaacggct gatcgtcctc cacgatcgcg cgcatctctg cctcgatcgc gggtaggtcg
     3121 ccttcgccga tcgcggtggg cagatcgaag tcgtagtaga agccgtcctg gatcggcggg
     3181 ccgatcgcgt acctcgctcc cggttgcagc cggcacaccg cctgtgccaa gacgtgggcg
     3241 gtggagtgcc ggaggatgtg gagcgcgtca tccgagcccg gcgcaaccgg ttccaccgac
     3301 gcgtcgccgg ccggcacgaa cgagagatcc cgcagggtgc cgtccacccg cgccgcgagc
     3361 gcgtccggag ccagcacggc gccgaccggc tccccctcgg ggagctcgac gtgggtaccg
     3421 tccggcaggc tcagtcgagg cacgagcgcc atgatatcgg cgagcctccg cgcctccgac
     3481 ggacgctact cgtacgagat cgcttccagg accttgatcc gcgacgcccg gatcgccggt
     3541 gcgatcgacg cgaagagccc gagcaggccg gcgataaccg cgaagacgac caggaggccc
     3601 catgggaccg cgagtcgcga cacgccgagg tctgcgagcg cttgctgcat cgcccagccg
     3661 aagaggagcc cgatcgcgat cccgagcacg gcgcccagca gggacacgat caccgcctcg
     3721 atcacgatca tccagccgat ctgcgtccgc tgcatgccga cggcgcggag gaggccgagc
     3781 tcgcggaccc gctcgtagat cgacagcgcc agggtgttcg cgatcccgaa ggcggagatg
     3841 atgaccgaca ggacgaggag caccaggacg atcccgaaga cctgatcgat caagcgatcc
     3901 tgctcctcct tgaactgcgc ttgatcgttg atctcgacgt tggggaactc acccgcgacc
     3961 tcttcgaggg cggctctcgc ctgctcgagg gagacgccgt ccgcccgtcg gacgaacacc
     4021 aggtagtcca actgctccgc gacgttggcc tggtactcct ccagcgagac ggtgtagtcg
     4081 ccgagcaatc ggttgtccgt gtagatgccg acgatcctca gctgcttcgt tcccgtccga
     4141 gcgaacgcca ccgggagctc gtctccgatc cccaggtcac gagcctgcgc gggacccttg
     4201 tagatcagga tcgtccccgg ctcgatcagc gccgccgttg tcccggcctc catcggcacg
     4261 ctcaccacgt cgtcgacggt tgtctcgtcc acaccggaga tgaaggtcag ccccccaccg
     4321 atcttcacac cggcctgccg gaagggcgac acggcggcga actcgtcgcg ctgcgacagc
     4381 gcgtcggcga tccccgggct gaagggctgg aacccggagg agctgaggat gaagtccgcg
     4441 cgcagggtct cgtcgagcgt gtcggacgcc gacgccttca gcgtcgtcat gaagacggac
     4501 acgaacacga ccagaccgag gccgatcatc agtgccgcgg ccgtggacgc ggtccggcgt
     4561 gggttgcgct tggcgttctc ttgcccgagg cgcccggtca tcccgaggcg ccgcgcggga
     4621 cctccgatcg cggtcgcgag cggcctcgcc acgaacgggg acagcaccgc gatcccgatg
     4681 aaggtgaact gggccccgag cccgacgatc gcggcgggct gcggcacgtc cccgaacaac
     4741 cccgcggcga tcgccccgac accggccgtc gtgagcagtc cacccacgat cgctcgacgg
     4801 cggagcgacg cggtcggagc gacggtcccc tcgcgcagcg cctcgatcgg tgcgacacgg
     4861 gaggcgcgac gcgccgggaa caccgacgcc acgacggtca cgacggtccc gaggacgagc
     4921 gcggcgatga tggtggtcgg tcggatcgcg agcggggtcg gtggcagctc cagcccgatc
     4981 agctcgaggc cctgcttgag caggaccgcg agcccgatcc ccgcggcgag cccgagggcg
     5041 gagcccagga gcccgatcac cagcgactcg atgatcacgg aggtgaacac ctgcccgcgg
     5101 ctggccccca gcgcccggag gagtcccatc tcacgggtcc gctgcgtcac cacgatgttg
     5161 aacgtgttga agatcacgaa ggccccgacg aacaacgcga cgaaggcgaa gacgagcagc
     5221 gccgtgcgga ggaaccccaa gccctcgcga acctgatccg tctgctccgc tgcggcatcc
     5281 gtcgccgtga tcgcctggaa gccggaggga cgcacctcct ggacctgcgc ggcgaccgac
     5341 gccgccgacg cgccctcccc tgcgacgatg tagatctgat cgtaggttcc ctcgcggtcg
     5401 aagaggcgtt gcgcggtctc gagctcgaac gacgcgactg tggcgccgcc gatcgtcgtg
     5461 gcggacggca gggagatcac gccggtgagt cggtacggat agacgccgag cggtgtgacg
     5521 atccggacgt cgtcaccgat ctccagaccc gcctcatcga cggtgtccgc gtcgatcacg
     5581 acctcgttct cggcctgcgg cggctccccc gtgagctcga acggcgtgac gtccggatcc
     5641 cacgagcttc cgatcgtcgg tgcgccgccg ctcgagatca cctcgtcggt ctgggggtcg
     5701 atcacctgcg cgaatccgcc cacgctgccg tccgccgcgg ccacacccgg cagcgcctgg
     5761 acatctccca ggacctcctc cgggatcggg ttccgctcct ggcctcctcc tcccccaccg
     5821 ccggcggcga tgtcctggaa cgcgttctcc gcctgcacga cgacgtcggt cttcgcgaag
     5881 atgtcgccga agagatcgtc gaatgcggac aacgcggtgt cggtgagcac gagcgttccc
     5941 gagacgaacc cgaccccgag cacgatggcc aacgccgtca ggacgagccg gagcttcctc
     6001 gcgagcacgc tcttcagggc ggctcgccac atctcagtgc ttcctcaccg tcgaacgcac
     6061 ctcacgcctc cagcaacttc atccggtcga ggacgcgctc cgcggtcggg tcgcgcatct
     6121 cgtcgacgac ctgaccgtcg gcgaggaaca cgatccggtt cgcgtagctc gcaccgacag
     6181 gatcgtgcgt gaccaccacg atcgtctgtc cgcgctcggt cacggcggcc ttgaggaacg
     6241 tgagcagctc ctgggacgca cgggagtcga ggttcccggt cggctcgtcc gcgaagacga
     6301 tctccgggcg cgagacgagg gctcgcgccg ccgcgacgcg ttgctgctgc ccgccggaga
     6361 gctcggaggg gaggtgcgtc agtcggtcgc gcagcccgat cgtgtcgacg acctcgtcga
     6421 agcgcgcccg gtcgacgtcc cgaccggcga tcgcggtagg aagcgtgatg ttctccgccg
     6481 ccgtgagcgt cggcaccagg ttgaacgcct ggaagacgaa gcccacgcga tcgcgccgga
     6541 tctccgtgag gtcgtgatcc ttgaggtgcg tgaggtcctg atcgccgatc cagacggtgc
     6601 ccgacgtcgg cttgtcgagc cccgccatgc aatgcaacag ggtggacttg ccggaaccgg
     6661 aggcgcccat gatcgcggtg tactccccgg cggcgaactc tacggagacc cctcggagcg
     6721 cttcgacggc ggccgtcccc tcgccgtaga ccttccggag atcgaccgcg cgtgcggctg
     6781 ccgtccgggt ttccgtgagc atcctgactc cccctcgaga cgcgtagccg gggagcttac
     6841 cggtacggga tgaacacttg tggtccgcgc gaacagggac tcagatctcg aaccacacct
     6901 cggtgccggc ggcgttccgc tcgatccccc agtcgtacgc cagcgtgtcg aggaggaaca
     6961 gaccaccccg gtggcccctc cagtcgatct ccgccgggtc gaagcccggg ccctgatcgc
     7021 agacacggcg acacgcacga agggaccggt gtcggtgatc gtgagcccga tccagcaccc
     7081 gagtactccg cc
//
LOCUS       DASUVE010000113         6844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1376512, whole genome shotgun sequence.
ACCESSION   DASUVE010000113 DASUVE010000000
VERSION     DASUVE010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376512"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VF036_02300"
     CDS             complement(<1..777)
                     /locus_tag="VF036_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264091.1"
                     /translation="MVLYALAALVVLPEGDPSLLMRLVSAAALVTLSALAAAVFRWGG
                     AWARGAAMLLIGFTMMAVAGGVVAKRLVDQVSLVDVLGVAASMSGVILVIVGWRLSLA
                     GIARRWARVAIAVLSTLLVAQFLLLPAGVALDVTNRPRPTGSSRTPADVDLRLQDVRI
                     RSADGTLLSAWWVAPRNGAAIVLLPGSGSTRHAVLEHAALVARHGYGALLLDWRGHGH
                     SGGRSLEFGWGAEADVRAAVSFVLSRAGVTRGVGVLGLSLG"
     gene            complement(891..1490)
                     /locus_tag="VF036_02305"
     CDS             complement(891..1490)
                     /locus_tag="VF036_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264092.1"
                     /translation="MTTMARTERPSVRRPLPLPPATVGRGGIPGWMGGLVVLLGFGLA
                     VWWGTATYVGMVDDLAELRRIQIPSARVVVLDEGTQVLSVESDRLAPFSDLRFAVVGP
                     DGAAVPVDRYEGDLAYDVPDRPERVGRAVATFETVSAGRYTVRVDGRAAPGAVLAIGE
                     DASRAALPEILGAMALMAASLVGGAIVLARRIARWADGR"
     gene            1638..3476
                     /locus_tag="VF036_02310"
     CDS             1638..3476
                     /locus_tag="VF036_02310"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HEX6264093.1"
                     /translation="MQVESFLVRFSPFDVLEPAALERVCRATEIEFFPRGATVLQQGG
                     PPSRFLYVVRTGGVELLDDGVVIDLLEEGEMFGHPSLVAGLPPTFSVRAFEDTICYLI
                     ERQVAEEVIGTRSGIEFLARALARRSRQAALASETERIARPPAPLSSILRREPIGCDP
                     TQSVRDAAATMAREAISSILVRADDLLGIVTDRDLRTKIVAEGIDPAVPVSRVMTTPV
                     ITVPEEAMADEALRMMLQHGIHHLPVVGSDGRVVGVVTDTDLIGLERSVPFTFLRALD
                     RAETTDEVFARMSELHTVVIPLVETEADPVDIGHLIASAIDATTRRLIHVAIAELGDP
                     PGPWAWLALGSEARHEQSLVTDQDHALVFVAPDVSPEEADSYFERLARRVTDGLAEAG
                     IPRCRGGVMAESAAWRADREAWIRTFREQLALSDVGGTAFSNIALDYRRVAGALDVEA
                     SIDQLVRQAAAEGWLVRKLASSALDLRPPTGFFREFLVGWDGAHAGTLDVKHGGITPI
                     TNVARTFAVASGVASNRTIDRLRAAASLGALDPDLAVGLEEAFRLLWRIRLRHQVRQL
                     RAGEPADDLADPRLLGPLTRRSLREAFKLIIAGQRVLSSTYGLRPR"
     gene            3473..4093
                     /locus_tag="VF036_02315"
     CDS             3473..4093
                     /locus_tag="VF036_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX6264094.1"
                     /translation="MIRLPERPRGFGGGSWRQAAYASLDFETTGLDLEGDDIISFGVV
                     PIEGGRILPGRSTYREVRPDRPLKPPSIAVHGLRPVDLEEASALWAVVDDLRDALAGR
                     YVLAWAAEIEAAFLARTFGRRPAWWRRRIIDVLRLALLVEESRSGRHADFSLTAVAHR
                     LGVPVEHPHHALDDALTTAEVFLVLQPELERRGITTPRRLLRAGRG"
     gene            complement(4127..4921)
                     /locus_tag="VF036_02320"
     CDS             complement(4127..4921)
                     /locus_tag="VF036_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6264095.1"
                     /translation="MIDLVRKELHDQRRALFGWGIGLSAMVLMYSSFWPSVRDNADQF
                     ASYLDNLPDALRSVIGELSLGTPVGYLQSELFSFLGPALILVFAIGAGARSLAGEEEQ
                     GTLDLLVVTPIPRGTIVLQKFAAMALTALGLGLVLWVSVVVVGRLFGLDVAVTDIAAA
                     TLHLLLLGVAFGAIAMFLGAWQGRRALAIGVTSAIAVGTFILNAFAATVDGFAWAARL
                     SPFFYYGDSLPLRNGLHIGHAGVLIALATLALIATVATFDRRDLRT"
     gene            complement(4918..5820)
                     /locus_tag="VF036_02325"
     CDS             complement(4918..5820)
                     /locus_tag="VF036_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6264096.1"
                     /translation="MTDGAAIALQGLTKRYGTRRGIEDVSLTVPPGEVFGFLGPNGAG
                     KTTTIRTMLDLLRPSAGRATLLGLDSHDDAVEIHRSIGYLPGDFVAYRRMTGRRYLEF
                     FAEVRDVPFGRVESLSRRFACDLEPQIDSLSHGNAQKLGLIQALMHRPRLLILDEPTQ
                     GLDPLIQRVFHELMREIAAEGRTVFLSSHVLPEVERMCDRVGIIGDGRLLGVEDVGDL
                     RAKAIRVLEFHFATPVPATVFADLTGVSDLEVHGESVRCSVRGPVDSVLKAAARYEVV
                     DLRSHEPGLEELFLEYYPTAEEAS"
     gene            complement(5817..6020)
                     /locus_tag="VF036_02330"
     CDS             complement(5817..6020)
                     /locus_tag="VF036_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264097.1"
                     /translation="MTGARPHPMSGHDAFRPGEIATSVETRGVAKAEAATITTFVLAL
                     VAAAFIAVAWFVSLRPVARGAER"
     gene            complement(6080..6649)
                     /locus_tag="VF036_02335"
     CDS             complement(6080..6649)
                     /locus_tag="VF036_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEX6264098.1"
                     /translation="MTRALVLFESMFGNTQQIAEAVKEGLSTSVPTDILEIGTAPGVI
                     PDGVGLIIVGGPTHAFGMSRPKTREDATRQAGGYVVSGGNGLREWITGLERAHGRNLA
                     AAAFDTRIAKPRVPGSAARGAEKRLRKLGFKIATSPESFYVTGTAGPLVDGETDRARR
                     WGEELGKGFMGDPDAAGTSEVVREPRVRG"
BASE COUNT         1102 a         2350 c         2329 g         1063 t
ORIGIN      
        1 tccgagcgac aggccgagga cacccacacc tcgcgtgaca cccgcccggc tcaggacgaa
       61 ggagacggcg gccctgacat cggcttccgc accccaaccg aactccaagc tccgccctcc
      121 gctgtggccg tgtccacgcc aatcgaggag gagcgccccg tacccgtgcc gggcgacgag
      181 agccgcgtgc tcgagtacgg cgtgtcgcgt cgaccccgat cccgggagca ggacgatcgc
      241 ggcaccgttc cgtggcgcga cccaccaggc cgagaggagc gtcccatctg cgcttcggat
      301 ccgtacgtcc tggaggcgga ggtcgacgtc cgcgggtgtc cggctcgatc ccgtcgggcg
      361 aggacggttc gtgacgtcga gagcgacacc cgccggcagg aggaggaact gcgccaccag
      421 cagtgtcgag aggaccgcga tcgcaacccg tgcccaccgt cgggcgatgc cggccaggct
      481 cagccgccaa ccgacgatca cgaggatgac ccccgacatg gaggcggcca cgcccagcac
      541 gtcgacgaga gacacctgat cgacgagacg cttggccacc acgcctcctg cgaccgccat
      601 catcgtgaag ccgatgagga gcatcgccgc tccccgcgcc cacgcgcccc cccaccggaa
      661 gaccgcggcg gcgagagcgc tgagggtgac gagagcggcc gcgctcacga ggcgcataag
      721 gagggatggg tcgccctccg ggagcacgac gagagcggcg agtgcgtaca ggaccaccgc
      781 tccggatgcg atgggggcga ggcgagacct ttcccgagcg ggcggtcgga tccggccctg
      841 cgaggggacg gcgctcaggc gagcggcggc tcgcgtttcg gccggggtgc tcatcgcccg
      901 tctgcccaac gagcgatcct ccgggcgagg acgatcgctc ctccgacgag ggaggcggcc
      961 atcagcgcca tcgcgccgag gatctccggg agcgcggctc gtgatgcatc ctcgccgatc
     1021 gccagaacgg caccgggcgc cgcccgaccg tccacccgga ccgtgtatcg gccagcggag
     1081 actgtttcga acgtcgccac ggcacgtcct actcgctcag gtcgatcggg gacgtcgtag
     1141 gccagatctc cttcgtagcg atccaccggc accgcggcgc cgtccggacc cacgacggcg
     1201 aatcgaagat cggagaacgg agccagccga tcgctctcca cggagaggac ctgggtcccc
     1261 tcgtcgagca cgaccacccg agcgctcggg atctggatcc gccgtagctc tgcgaggtcg
     1321 tcgaccatcc cgacataggt cgccgttccc caccagacgg cgagtccgaa gccgaggagc
     1381 acgacgagtc ctcccatcca cccggggatg cctccgcgac ccacggtcgc gggagggagc
     1441 gggagcgggc gacggacgga gggccgttcg gttcgtgcca tcgtggtcat cggagtgcct
     1501 ccttgcccgg aagtccggtc tcatccgagt ctgttcgctg tatgcggttc tggaggtgtc
     1561 gaaggtcccg tcgtccgggg gccggtcgcc cgcgggatcc tgggccggag gatcagcgtg
     1621 ggtgatactt ccgcaggatg caggtcgagt cgttcctcgt tcgcttctca cccttcgatg
     1681 tcctcgagcc ggccgcgctc gaacgtgtct gcagggccac cgagatcgaa ttcttccccc
     1741 gcggagcgac ggtcctgcag cagggagggc ccccctcgag gttcctctac gtggtgcgga
     1801 ccgggggcgt ggagctcctc gacgacggag tcgtgatcga cctcctggaa gagggcgaga
     1861 tgttcggaca tccctcgctc gtcgccggcc tcccgccgac cttcagcgtt cgagcgttcg
     1921 aggacacgat ctgctacctc atcgagcggc aggtcgccga agaggtcatc gggacccggt
     1981 cgggcatcga gttcctcgct cgagcactcg cccgccggag ccggcaagcg gcactcgcct
     2041 ccgagacgga acggatcgct cgccctcccg caccgctctc gtcgatcctg cgcagggagc
     2101 cgatcggatg cgacccgacg cagtccgtca gggatgcggc ggcgacgatg gcgcgcgaag
     2161 cgatctcgtc gatcctcgtc cgtgcggacg atctgctcgg catcgtgacg gatcgggatc
     2221 tccggaccaa gatcgtcgcc gaaggcatcg atcccgccgt gcccgtctcc cgggtcatga
     2281 ccacgcccgt gatcaccgtc ccagaggagg cgatggcgga cgaggccctc cggatgatgc
     2341 tgcaacacgg tatccaccac ctgccggtag tgggttcgga tggacgcgtc gtgggtgtcg
     2401 tgaccgacac cgatctgatc ggactcgagc ggagcgtccc gttcacgttc ctccgcgccc
     2461 tggatcgtgc ggagacgacc gacgaggtgt tcgcgcggat gtccgagctt cacaccgtcg
     2521 tgatcccgct ggtggagacg gaggctgacc ccgtggacat cgggcacctc atcgccagcg
     2581 cgatcgatgc caccacgcga cggttgatcc acgtggcgat cgcggagctc ggggatccgc
     2641 ctggcccatg ggcgtggctc gccctcggga gcgaggcccg tcacgagcaa tcgctcgtga
     2701 ccgaccagga tcacgcgttg gtcttcgtgg cacccgatgt gtctccggag gaggctgact
     2761 cgtacttcga acggctcgcg cgacgcgtga ccgacgggct cgccgaggcg gggatccccc
     2821 gatgtcgagg cggcgtcatg gccgagtccg cggcgtggcg agcggatcgg gaggcgtgga
     2881 tccgaacgtt ccgcgaacag ctggcgctct ccgacgtcgg tgggacggcg ttctcgaaca
     2941 tcgcactcga ctaccgccgg gtcgcgggcg cgctcgacgt ggaagcgtcc atcgaccagc
     3001 tcgtccggca ggccgcggcg gagggatggc tcgtccggaa gctcgcctcg tcggccctcg
     3061 atctccgacc cccaaccggc ttcttccgtg agttcctggt ggggtgggac ggtgcacatg
     3121 ccgggacgct cgatgtgaag catgggggga tcacccccat cacgaacgtc gcgaggacgt
     3181 tcgcggttgc gtcgggggtc gcatccaacc ggacgatcga ccgcctccgc gccgctgcat
     3241 cccttggggc gttagatccc gacctggccg ttgggctcga ggaggcgttc cgcctgctgt
     3301 ggcggatcag gctgcggcac caggtgcgtc agcttcgcgc gggagagcct gcggacgacc
     3361 tcgcggatcc ccgcctcctc ggccctctga cccgcaggag cctgcgggaa gctttcaaac
     3421 tgatcatcgc gggccagcgc gtgctctcgt ccacgtacgg gttgcgcccg cgatgatccg
     3481 cctcccggag cgccctcggg gtttcggagg cggatcgtgg cgacaggccg cgtacgcatc
     3541 gctcgatttc gagacgacgg gcctcgacct cgagggggac gacatcatct cgttcggcgt
     3601 ggtaccgatc gaagggggga ggatcctccc ggggaggtcg acctatcgcg aggtccggcc
     3661 ggatcgaccg ttgaagcctc catcgatcgc ggtgcacggt ctgcgcccgg tggacctgga
     3721 agaagcgtct gccctctggg cggtcgtgga cgatctgcgc gacgccctgg cgggacggta
     3781 cgtgctcgcg tgggccgcgg agatcgaagc ggcgttcctg gcgaggacgt tcgggcgtcg
     3841 gcccgcgtgg tggcgaaggc ggatcatcga cgttctccga ctggctctcc tcgtcgagga
     3901 gtctcgctca ggtcgccacg ccgacttctc gctcaccgcc gtcgcccatc ggttgggcgt
     3961 tccggtggag catccgcatc acgcgctcga cgatgcattg acgaccgcgg aggtcttcct
     4021 cgtcctgcag ccggagctgg agcggcgagg gatcacgaca cctcgacggc tacttcgtgc
     4081 cggtcggggg tgagggcgac atccccccgc cgcgagtcga gccaggtcaa gtcctcaggt
     4141 cacgtcggtc gaacgtggcc accgtcgcga tcagagcgag ggtggcgagg gcgatcagaa
     4201 ctccagcgtg accgatgtgg aggccgttgc ggagcggaag gctgtcccca tagtagaaga
     4261 aggggctcag gcgcgcggcc cacgcgaacc catcgacggt ggcagcgaac gcgttcagga
     4321 tgaacgttcc gacggcgatg gccgaggtga caccgatcgc gagggcgcgt cgaccctgcc
     4381 acgctccgag gaacatcgcg atcgcgccga acgccacccc gaggagcagc aggtgcaagg
     4441 tcgctgccgc gatgtcggtt accgcgacat cgagtccgaa gagccgaccg accaccacga
     4501 cggagaccca cagcaccagg ccgaggccga gcgccgtcag ggccatcgcg gcgaacttct
     4561 gcaggacgat cgtccctcgg gggatcggag tgacgacgag gaggtccaac gtcccctgct
     4621 cctcctcccc ggcgaggctc cgcgcccccg ctccgatcgc gaacacgagg atcaaggccg
     4681 gcccgaggaa cgagaacagc tcgctctgga gatagccaac cggtgtcccc aggctgagtt
     4741 cgccgatcac gcttcggagg gcatcgggga ggttgtccag gtaagacgcg aactgatcgg
     4801 cgttgtcacg cacgctgggc cagaaggagc tgtacatcag caccatcgcc gagaggccga
     4861 tcccccatcc gaaaagcgct ctccgctggt catggagctc cttccgcacg agatcgatca
     4921 cgacgcttcc tcggctgtgg ggtagtactc gaggaacagc tcctcgagac ccggttcgtg
     4981 gctccggagg tcgacgactt cgtaccgggc agccgccttg agcacgctgt cgacggggcc
     5041 acggaccgag cagcggacgg attccccgtg gacctccaga tcggagacgc ccgtgagatc
     5101 cgcgaacacg gtggccggca ccggcgttgc gaaatggaac tcgagaaccc ggatcgcctt
     5161 ggctcggagg tcgccgacat cctcgacgcc cagcaacctt ccgtctccga tgatccccac
     5221 ccggtcgcac atccgctcca cctcgggcag gacgtgggag gagaggaaca cggtcctgcc
     5281 ctccgccgcg atctcccgca tcagctcgtg gaacacacgc tggatcaggg gatcgaggcc
     5341 ctgggtcggt tcgtcgagga tcaggaggcg gggtcgatgc atcagggctt ggatcaagcc
     5401 gagcttctgc gcgttcccat gggacaacga gtcgatctgt ggttcgaggt cgcatgcgaa
     5461 cctccggctg aggctctcga cccgtccgaa cggcacgtcg cgcacctccg cgaagaactc
     5521 caggtagcgg cgcccggtca tccgtcgata cgcgacgaag tctccgggga gatagccgat
     5581 cgatcggtgg atctccaccg cgtcgtcgtg ggagtcgagg ccgaggagcg tggctcgtcc
     5641 tgcgctcggc cgaaggagat cgagcatcgt tctgatcgtc gtggtcttcc ccgcgccgtt
     5701 cggtccgagg aacccgaaca cctcgcccgg agggaccgtg aggctgacgt cctcgatgcc
     5761 acggcgcgtt ccatagcgct tcgtgagccc ttgaagtgcg atcgccgcgc cgtccgtcac
     5821 cgctcggctc ctcgggctac gggccgcagg gagacgaacc atgccaccgc gatgaacgcc
     5881 gcggcgacga gcgcgagaac gaacgtcgtg atcgtcgcgg cctccgcctt cgccacgcca
     5941 cgggtttcca ccgaggtcgc gatctccccg ggcctgaagg catcatgccc ggacatcggg
     6001 tgcgggcggg ctcccgtcat cgccacatca tgccgtctct tctcctcggc ttgtcgggag
     6061 gacatcgatg gcactcgagt cacccccgga cgcgaggctc tcggacgacc tccgaggtcc
     6121 cagccgcgtc gggatcgccc atgaacccct tgccgagctc ttcaccccat cgccgggcgc
     6181 ggtccgtctc cccgtcaacg agcggaccgg ccgtccccgt cacgtagaag ctctccggcg
     6241 aagtggcgat cttgaaaccg agcttccgga gacgcttctc ggcgccgcga gcggccgaac
     6301 caggcacgcg aggcttggcg atccgtgtgt cgaatgcggc agccgcaagg ttgcggccgt
     6361 gggctcgctc gaggccagtg atccactcac gcagcccgtt cccgccggac acgacatatc
     6421 cgccggcttg gcgggtcgcg tcctcacggg tcttgggccg gctcatgccg aacgcgtgtg
     6481 tgggacctcc cacgatgatc aggccgacgc cgtccgggat gactccgggt gcggttccga
     6541 tttcgaggat gtcggtcggc acggaagtgg agagtccctc cttcaccgcc tcggcgatct
     6601 gctgagtgtt gccgaacatg gactcgaaca gcaccagtgc gcgtgtcatg tcatccctcc
     6661 tgtgaaccgc tccgcggtgc acggtccttc gggaccgtcc agaccgtcga tcgacgcgaa
     6721 gaaggcgggg ccttcgggcc ccgccttgct cgccttcgga tctatcagta caggtacctc
     6781 cggtcctgca cgaatccgac ccgctgccag accttgccga ggccgtagta gcgtccggcg
     6841 ttcg
//
LOCUS       DASUVE010000114         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_246675, whole genome shotgun sequence.
ACCESSION   DASUVE010000114 DASUVE010000000
VERSION     DASUVE010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246675"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /gene="purH"
                     /locus_tag="VF036_02340"
     CDS             complement(<1..610)
                     /gene="purH"
                     /locus_tag="VF036_02340"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019159103.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX6264099.1"
                     /translation="MTDVREVRRALLAVYDKAGVVELARALVELGVSLVSSGGTAATL
                     RDAGVPVTPVEEVTGFPEMLDGRVKTLHPRIHGGLLADRRKPEHLEQLAAHGIEPFDL
                     VVVNLYPFRETVASGAGADDVIEKIDIGGPAMVRAAAKNFESVGVVVDPARYPTIVEE
                     LRARGGLSRDTRERLAATAFAHTAAYDAAVAGWFAERAAETDL"
     gene            complement(607..1227)
                     /gene="purN"
                     /locus_tag="VF036_02345"
     CDS             complement(607..1227)
                     /gene="purN"
                     /locus_tag="VF036_02345"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX6264100.1"
                     /translation="MRTSFAERIAVLVSGSGTNLQALLDHPVCGPRIALVLSDRPGVK
                     AIERAESHGVEWAVLDPKDVDGREAFDRAVADLLRDRRIDAMVTAGYMRLLGPAVLDA
                     YAGRWLNVHPSLLPAFPGMHGVADALAHGVKLTGVTVFLVDEGTDTGPIVLQEAIEVF
                     DDDDWDSLEARVHAVEHRLLPAATAALIEGRIRIEGRHVRIEGGTT"
     gene            1281..1952
                     /locus_tag="VF036_02350"
     CDS             1281..1952
                     /locus_tag="VF036_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HEX6264101.1"
                     /translation="MEADERIRATPEAPRPATEARERDETAASTEASGGVTHERCVHH
                     PGRAAVARCSACEEPICLSCAVPVRGRVIGPECLAAELGDPQLAVPPETDEAAPGSVA
                     AVAGAVVAVVATIGPWTRTGAGDRVLGAWVPSIRWSMIAALAAVALLVVALWFRARGT
                     RTTATLVIVVGAAVASGSVLAIAFPPTFQEASWGPWLGAVGGVVAAMGAIASISAERR
                     PRQGV"
BASE COUNT          315 a          792 c          775 g          313 t
ORIGIN      
        1 ggagatccgt ctctgccgcc cgctcggcga accaacccgc caccgcggcg tcgtaggccg
       61 cggtatgcgc gaacgcggtc gccgcgaggc gctcgcgcgt atcgcgggac agcccgcctc
      121 gtgcgcggag ctcctccacg atcgtcgggt agcgcgccgg atccaccacg acgcccaccg
      181 actcgaagtt cttggcggcg gcacgcacca tcgcgggccc gccgatgtcg atcttctcga
      241 tcacgtcgtc ggcacctgcc ccggaagcga ccgtctcccg gaaggggtag aggttgacga
      301 cgacgaggtc gaacggttcg atcccgtgcg cggcgagctg ttcgaggtgc tcgggtttcc
      361 ggcggtccgc cagcaacccc ccgtggatcc gcggatggag cgtcttgacc ctgccgtcga
      421 gcatctcggg gaagcccgtc acctcctcga cgggcgtgac cggcaccccg gcgtcgcgga
      481 gcgtggcggc cgtcccgccg gaggacacaa gcgagacgcc gagctcgacc aacgctcgag
      541 caagctccac cacgcccgcc ttgtcgtaga ccgcgagcag ggcccgccgc acctcccgga
      601 cgtccgtcac gtcgtccctc cctcgatcct cacgtgccgc ccctcgatcc ggatgcggcc
      661 ctcgatgagt gccgcggtcg ccgccggcag cagtcggtgc tccaccgcgt gaacgcgcgc
      721 ctcgagcgag tcccagtcgt cgtcgtcgaa gacctcgatc gcctcctgca ggacgatcgg
      781 accggtgtcc gtcccctcgt cgacgaggaa caccgtcacc cccgtcagct tcaccccgtg
      841 ggccagagcg tccgcgacgc cgtgcatccc cgggaacgcg ggaaggagcg aggggtggac
      901 gttcagccaa cggccggcgt aggcatcgag gaccgccggt cccaacaggc gcatgtaccc
      961 ggcggtcacc atcgcgtcga tcctccgatc gcggaggaga tcggccacgg cacggtcgaa
     1021 cgcctctcga ccatcgacgt ctttcgggtc gaggacggcc cactcgacgc cgtgggactc
     1081 cgcacgctcg atcgccttga ccccgggacg atccgacagg acgagggcga tccgggggcc
     1141 gcagacgggg tgatcgagga gggcttgcag gttcgtcccg gagccggaaa cgagcaccgc
     1201 gatccgctcg gcgaacgacg tcctcatcgg cgcggccacg ctatcagagg caccgaccgg
     1261 ccaggccgta cgctatcgcc gtggaagccg acgagcggat ccgcgcgacc ccggaggcgc
     1321 cccggcccgc caccgaggca cgcgagcgcg acgagacggc ggcgagcacc gaggcgtccg
     1381 gcggcgtcac acacgagcgg tgcgtccatc atccgggcag agcggccgtc gcccgctgca
     1441 gcgcgtgcga ggaaccgatc tgcctctcct gtgcggtccc ggtccgaggc cgtgtgatcg
     1501 gtcccgaatg cctggccgcg gagctcgggg acccgcagct ggccgtccct ccggaaaccg
     1561 acgaagcggc ccccgggtcg gtggcggccg tcgccggagc cgtcgtggcc gtcgtggcca
     1621 cgatcggtcc ctggacgagg acgggcgccg gcgatcgcgt gctcggtgca tgggtcccga
     1681 gcatccggtg gtcgatgatc gcggccctcg cagccgtcgc tcttctcgtg gtggcgttgt
     1741 ggttccgggc ccgcgggacc aggacgacgg cgacgctcgt gatcgtcgtg ggcgccgcgg
     1801 tggcatcggg atcggtgctc gcgatcgcgt tcccgcccac cttccaggag gcctcgtggg
     1861 gaccctggct cggcgccgtc ggcggtgtgg tcgcggcgat gggagcgatc gcgagcatct
     1921 cggcggaacg gcgtccccgg cagggtgttt gactccagtc tcgggcccct cttggatagc
     1981 gggagagatg cagccgcgcg agcgcgcggc ggtcgaaagg agcgatgcat ggaaggttcg
     2041 ggctcgtccg gcttcgacgt gtcgaagatg tcgaccgccg agaagctcct cgcgggaggc
     2101 agcatcctgc tcctgatcga cagcttcctc ccctggcagc aggtatgcgc gtcagacgtc
     2161 ctcggtgact tgggcgacct cgccggattc gacgc
//
LOCUS       DASUVE010000115         4574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_623739, whole genome shotgun sequence.
ACCESSION   DASUVE010000115 DASUVE010000000
VERSION     DASUVE010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623739"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VF036_02355"
     CDS             <1..662
                     /locus_tag="VF036_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX6264102.1"
                     /translation="GIERADSIAFDPHKWLYTPLSGGCVVVRDMELMRRAFDVDYVAY
                     VVKDEEHTDWGIDLGRNSPNFSRGFWALKVWVSLLAHGRTAYGRRISHDAALARYLGA
                     RVEESDEFELMTPVGLSITCFRYVPADLSSRGPRLDPGGPDDLGRDAYLDLLNNRLMT
                     EIQLDGRVYCSNAVLGERFCLRSCIVNYRTEAEDMDALLDVASELGAKLDVELRPEAL
                     R"
     gene            659..1372
                     /locus_tag="VF036_02360"
     CDS             659..1372
                     /locus_tag="VF036_02360"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX6264103.1"
                     /translation="MTWWSLLGSGEFQPWSEPVDRRLVEEADGDGRVLILPTASAPEG
                     KDVFERWGSLGLAHFSRLEIVAEVLELKTRDDTDDPRLIERLEGASAVYLSGGNPAYL
                     ASTLTESDFLDALVKQLDRGMAYAGCSAGVACLNEVTFDSDTTDVDQIFKPGLNLFPR
                     TLFAPHWDMVDTWMPGAAEFLVASAPEGSTFIGMDEDTAMVGDGAAWEVFGKSGIHVR
                     VGGDWTTYRDGERFDLALV"
     gene            1386..1997
                     /locus_tag="VF036_02365"
     CDS             1386..1997
                     /locus_tag="VF036_02365"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6264104.1"
                     /translation="MDEMYHDGNRELQDRFDTRRLADRIEQFLVHATFTERDRAFVDS
                     RDMFFLATADEDGKPNVSYKGGDPGFIRVIDEHTLAFPNYNGNGMYLSMGNLRKNPHV
                     GILFMDFEGRDRMRVNGEASVDFDDELRAFWPEAQFVVRVRAREIFPNCPRYVHRYQK
                     VAASVFVPREDCETPVPSWKRADWAVDVLPENDPALDPDATVM"
     gene            2022..2939
                     /locus_tag="VF036_02370"
     CDS             2022..2939
                     /locus_tag="VF036_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX6264105.1"
                     /translation="MNVTVAAVQATPEFLDRDATVQKVVRLTKEAAGLGARLIVFPET
                     FVPTYPDWVWRAAAWDGPFEALTARLREQSVEVPSPATEELGKAAKRAKAYLSVGVNE
                     LDGSTIYNAQLLFGPDGEIVRKHRKLMPTGGERLVWGLGDGSDLDPVETPFGRVGGLI
                     CWENMMPLARTAIYAKGVDIWTAPTWDNDENWIANLRHIAREGRVYVIGVCTLLRGSD
                     IPDDVPGRELWGGEDDWANPGWSAIVDPDGQLLAGPLVEEEGILTAEIDAQVARAQRY
                     RFDPVGHYSRPDVFTLLVNDAPKRSVTSE"
     gene            2949..3611
                     /gene="nth"
                     /locus_tag="VF036_02375"
     CDS             2949..3611
                     /gene="nth"
                     /locus_tag="VF036_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX6264106.1"
                     /translation="MPAKQQLTRDDPIEERAPEIIRRLSEAYPDAQVALHFTNPLECL
                     VATVLSAQSTDETVNKVTETLFQKYRTAEDYLRVPEDELKADIHATGFFNQKAISIRE
                     ACRRIVEEYGGEVPGTMEDLITLRGVARKTANIVLGNSFGIVEGIAVDTHVNRLANRL
                     GFSEQKNPDKIEQDLMRLVPKEQWFDFTYVLIDHGRALCTAKNPKCPECPVEPLCPAS
                     QA"
     gene            3608..4351
                     /locus_tag="VF036_02380"
     CDS             3608..4351
                     /locus_tag="VF036_02380"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX6264107.1"
                     /translation="MNVDRPAKPLVVAHRGASVEYPENTIEAFEAAIELGADAVEFDV
                     RMTADGHAVVMHDPDVSRTTDGTGLVSEMTLDEIRTLGVPTLEEALACLSGRAAADIE
                     VKNLPREPGFTPEREAAVEATLIALDAAAFSGQVIVSSFNPASIAHSRALRPDVPTGL
                     LTQYDVDAEEALARATSHGHPWVLPFVLKVLEAGDGVADRVHAGGALLGVWIADDPET
                     ARRLFEIGADAVATNDPRGIVPVRDETVP"
     gene            4352..>4574
                     /locus_tag="VF036_02385"
     CDS             4352..>4574
                     /locus_tag="VF036_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264108.1"
                     /translation="MFRRKRLSPDLDAALADLRAVVEHVEAAKLAMTGTVPSTRSAGT
                     PLAEGILELEQRLTAAQELMPTWRHADVEA"
BASE COUNT          739 a         1524 c         1620 g          691 t
ORIGIN      
        1 acgggatcga gcgggccgat tcgatcgcgt tcgatcctca caagtggctg tacacaccgc
       61 tcagcggcgg gtgcgtggtc gtccgggaca tggagttgat gcggcgggcg ttcgacgtcg
      121 actacgtcgc gtacgtggtc aaggacgagg agcacacgga ctggggcatc gacctcggac
      181 ggaactcgcc caacttcagc cggggcttct gggcgctgaa ggtatgggtg tcgctcctcg
      241 cgcacggccg gacggcctac gggcgccgga tctcccacga cgccgccctc gctcgctacc
      301 tcggcgcgcg ggtggaggag tcggacgagt tcgagctgat gacgccggtg gggctgtcga
      361 tcacgtgctt ccgctacgtg ccggcggacc tctcttcacg cggtccgcgc cttgaccccg
      421 ggggtccgga cgacctcggc cgagacgcgt acctcgacct gctgaacaac cggctgatga
      481 ccgagatcca gctggacggc cgcgtgtact gctcgaacgc cgtgctcggc gagcgattct
      541 gcctccggtc gtgcatcgtg aactaccgga cggaggcaga ggacatggat gcgctgctgg
      601 acgtggcgtc cgagctcggc gcgaagctcg acgtcgagct ccgtcccgag gccctgcgat
      661 gacctggtgg tcgctcctcg gctcggggga gttccaacct tggtcggagc cggtcgaccg
      721 ccggctggtc gaggaggcgg acggtgacgg tcgggtgctg atcctcccca ccgcgtccgc
      781 ccccgagggg aaggacgtgt tcgagcgctg gggatcgctc ggtctcgccc acttctcgcg
      841 actggagatc gtcgccgagg tcctggagct caagacgagg gacgacacgg acgatccgcg
      901 gctgatcgaa cggctggagg gcgcgtccgc cgtctatctc tccggcggga accccgcgta
      961 cctggcatcc acgctcaccg agagcgactt cctcgacgcg ctcgtgaagc agctcgaccg
     1021 cgggatggcg tacgcgggct gcagcgccgg cgtcgcatgc ctcaacgagg tcacgttcga
     1081 cagcgacacg accgacgtcg atcagatctt caagccggga ctgaacctgt tcccgcggac
     1141 gctcttcgca ccgcactggg acatggtgga cacgtggatg ccgggagcag cagagttcct
     1201 cgtcgcgtcc gcgccggagg gttccacctt catcgggatg gacgaggaca cggccatggt
     1261 cggcgacggc gcggcctggg aggtcttcgg caagagcggc atccacgtac gggtcggcgg
     1321 cgattggacg acgtatcgag acggcgagcg gttcgacctc gcgctcgtct gaggggtggg
     1381 cagagatgga cgagatgtac cacgacggca accgtgagct gcaggatcgc ttcgacaccc
     1441 gcaggctcgc cgatcggatc gagcagttcc tcgtccacgc cacgttcacc gagcgggatc
     1501 gcgcgttcgt cgattcgagg gacatgttct tcctcgccac cgccgacgag gacggcaagc
     1561 cgaacgtgag ctacaagggc ggtgaccccg ggttcatccg ggtgatcgac gagcacacgc
     1621 tcgcgttccc gaactacaac ggcaacggga tgtacctgtc gatggggaat ctgcggaaga
     1681 acccccatgt gggcatcctg ttcatggact tcgaggggcg cgaccggatg cgcgtcaacg
     1741 gcgaggcttc cgtggacttc gacgacgagc tccgcgcctt ctggccggag gcgcagttcg
     1801 tggtccgcgt gcgtgcgcgg gagatcttcc cgaactgccc gcgctacgta caccgctatc
     1861 agaaggtggc ggcctcggtc ttcgtccccc gtgaggactg cgagacgccc gtgccgtcct
     1921 ggaaacgtgc cgactgggcc gtcgacgtgc ttcccgagaa cgaccccgcc ctcgatccgg
     1981 atgcgaccgt gatgtgagat gaccgttgag gagtgctcgc catgaacgtg accgtcgccg
     2041 ccgtccaggc cacaccggag ttcctcgacc gcgacgccac ggtgcagaag gtggtgcgcc
     2101 tgacgaagga ggccgccggc ctgggagcgc gtctgatcgt gttcccggag acgttcgtcc
     2161 cgacctatcc ggattgggtg tggcgggcgg ccgcgtggga cggcccgttc gaggcgctca
     2221 cggcgcgctt gcgcgagcaa tcggtcgagg tgccgtcacc ggcgacggag gagctcggga
     2281 aggccgcgaa gcgcgcgaag gcctacctct cggtcggcgt gaacgagctt gacggcagca
     2341 cgatctacaa cgcgcagctg ctgttcggcc ccgacggcga gatcgtgcgg aagcaccgca
     2401 agctgatgcc gaccggcggc gagcgtctcg tctggggcct cggcgacggg tccgacctcg
     2461 atcccgtgga gacgccgttc gggcgcgtgg gcgggctgat ctgctgggag aacatgatgc
     2521 cgctcgcgcg gaccgcgatc tacgcgaagg gcgtggacat ctggacggcc cccacctggg
     2581 acaacgacga gaactggatc gcgaacctcc gccacatcgc acgcgaaggg cgcgtctacg
     2641 tgatcggcgt gtgcacgctg ctgcggggga gcgacatccc ggacgacgtt cccggccgcg
     2701 agctctgggg cggcgaggac gactgggcga acccaggatg gtcggcgatc gtcgaccccg
     2761 acggccagct cctcgcggga ccgctggtcg aggaggaggg gatcctcacc gcggagatcg
     2821 acgcgcaggt cgcccgggcg cagcggtacc gcttcgatcc cgtcggccac tacagccggc
     2881 ccgacgtgtt cacgctgctc gtgaacgatg cgccgaagcg gtcggtgacg tccgagtagc
     2941 atccgggcgt gcccgcgaag cagcagctga cccgcgacga cccgatcgag gagcgcgccc
     3001 ccgagatcat ccggcgcctc tccgaggcct accccgacgc ccaggtcgcc ctccatttca
     3061 cgaaccccct cgagtgcttg gtcgcgacgg tcctctccgc ccagtccacc gacgagaccg
     3121 tgaacaaggt caccgagacg ctgttccaga agtaccggac cgccgaggac tacctgcgcg
     3181 tgcccgagga cgagctcaag gccgacatcc acgcgacggg gttcttcaac cagaaggcga
     3241 tctcgatccg cgaggcgtgc cggcggatcg tcgaggagta cggcggcgag gtgcccggca
     3301 cgatggagga cctgatcacc ctccgaggcg tcgcgcggaa gacggcgaac atcgtcctcg
     3361 ggaactcgtt cgggatcgtc gaggggatcg cggtcgatac ccacgtgaac cggctcgcca
     3421 atcggttggg gttcagcgag cagaagaacc ccgacaagat cgagcaggat ctcatgcggt
     3481 tggtcccgaa agagcagtgg ttcgacttca cgtacgtgct gatcgaccac ggccgcgcgc
     3541 tctgcacggc gaagaacccg aagtgtcccg agtgcccggt cgagcccctg tgcccggcca
     3601 gccaggcgtg aacgtcgacc gcccggcgaa gccgctggtc gtggcgcacc gtggcgcctc
     3661 cgtcgagtat cccgagaaca ccatcgaggc gttcgaggcg gcgatcgagc tcggggctga
     3721 cgccgtcgag ttcgacgtgc ggatgacggc cgacgggcac gccgtcgtca tgcacgaccc
     3781 cgacgtgtcg aggacgaccg acgggaccgg gctcgtctcg gagatgacgc tggacgagat
     3841 ccggacgttg ggcgtgccga cgctggagga ggcgctcgcg tgcctgtccg gccgtgccgc
     3901 cgccgacatc gaggtcaaga acctgccgag ggagccgggc ttcaccccgg aacgcgaggc
     3961 ggcggtggag gccacgctca tcgcgctcga cgccgctgcg ttctcgggtc aggtgatcgt
     4021 ctcgagcttc aatccggcct cgatcgcgca cagccgcgcc ctgcgaccgg acgtcccgac
     4081 cgggttgctc acccagtacg acgtggatgc cgaggaagcc ctcgcacgag ccacatcgca
     4141 cggccatccg tgggtcctgc cgttcgtcct gaaggtcctg gaggcgggcg acggcgtcgc
     4201 cgaccgggtc cacgccggcg gcgcgctcct cggcgtgtgg atcgccgacg atcccgagac
     4261 cgcgcgccgc ctcttcgaga tcggagccga cgcggtcgcc acgaacgacc cgcgcgggat
     4321 cgttccggtc cgcgatgaga ccgtccccta ggtgttccgt cggaagcggc tctcgccgga
     4381 cctggacgcg gctctcgccg atctccgagc cgtggtcgag cacgtcgagg cggcgaagtt
     4441 ggcgatgacc ggcaccgtcc cgtcgacgcg gtcggcgggg acgccgctcg cggaggggat
     4501 cctcgagctg gagcagcggc tcacggccgc gcaggagttg atgcccacct ggcggcacgc
     4561 cgacgtcgaa gctg
//
LOCUS       DASUVE010000116         5633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1001365, whole genome shotgun sequence.
ACCESSION   DASUVE010000116 DASUVE010000000
VERSION     DASUVE010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001365"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VF036_02390"
     CDS             <1..768
                     /locus_tag="VF036_02390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6264109.1"
                     /translation="GDLAARLAEAEEVMRKARGDYERLEMELRALSPSGAATTGSAVW
                     TEIPDGYYWAFERRMRGAAGSIEDRLRGYEDLVVPHREAVGTTDDRRPRWVDLGCGQG
                     EFCALLGEWGWDAEGVDASPDAVEACRARGFDVTLADVLNYLETRPDDPVGGVSAIQL
                     IEHLPRRAWVHLFQEIHRALAPGGALLLETINGQNPDAIADYFVADVTHTWPGHPETL
                     RLMAEHAGFDEVQVRFLHEDHRGRAQDVAIWAVKASS"
     gene            765..1211
                     /locus_tag="VF036_02395"
     CDS             765..1211
                     /locus_tag="VF036_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264110.1"
                     /translation="MIDPRDGKEDAPGGSTPAEPAPTAPENQDDAVASLAREVYWAGE
                     GGSSGLPADARTLRERLDAFTYQMRADQRQAVPVGETFLGSDSGWKRGLKQFVWRLTR
                     FSTMRYDRLLAELAEMNGELARRLVATEEELSRLRRRLDHRDGDAR"
     gene            1208..2257
                     /locus_tag="VF036_02400"
     CDS             1208..2257
                     /locus_tag="VF036_02400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6264111.1"
                     /translation="MRVAVLHPQTAFTRGGAELHTEALVRALRESGHDAEEVTIAGKW
                     YPATELAHQMAVWRSFDISESNGLKVDAVIALKFPAYLAAHERKIVWLIHQHRSAYEL
                     WDHPDFADLSRQDEGPLIRRLVWNSDRLGLGEAKQVFTNSKNVAERLWTSLGLKGDVL
                     YHRSPATEALLARDPEELGHYLFYPSRLEILKRQSLAIDAMRHVTTPVKLVLVGRGPD
                     ERSLRERIAAHGLEDRVRLEVGVSDERLHELYRGALGVYFGPFDEDFGYVTTEGFAAA
                     RPVVTLTDSGGPLEFVRHEESGLVARPEPKAIAAAFDRLWSDRDAARAWGLEGHRLVR
                     ETVPEWPAVVARLLG"
     gene            2254..3624
                     /locus_tag="VF036_02405"
     CDS             2254..3624
                     /locus_tag="VF036_02405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6264112.1"
                     /translation="MKPTRRLAGRVARRVGLRRPAGSGRRTIARRTNGRLAFVGPMPP
                     ASTGIASYDQAVMDGLRRTGFLHRHRTDVIWPVRSSQDRRIAAYDLGIFQLGNNARFH
                     GQIYRLAIASPGLVVLHDLALDGLVLGLAAQGDPAAIATAREAAALRSRVTDPDIAMH
                     EPLRSVWCAAVARASRGIVVHSPFGKRYLESVGVRTPIFTVPHPVVEDETTMRRAEPR
                     AKELRAMLAAQGARTIVVAPGDVNEAKCLPALVEAIATLPGDVHAALVGRRIPGTDIT
                     AAIEAYGIGDRVTVHHDVSDDDFRAWLVAADIAVDLRFPHRGEVSGSLSMAMVAGTPT
                     IVSATGTYLDIPEELVVRVAPGPADPAELARRILELRDEPERRARVGSAARAHMAALR
                     DTEATARGYEEAIVATRAIVAHPTRPLRARWAASLVAVGIDDTLVEEGYGVSYVRALE
                     SFERTP"
     gene            3635..4627
                     /locus_tag="VF036_02410"
     CDS             3635..4627
                     /locus_tag="VF036_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6264113.1"
                     /translation="MRRRAIARLATVALTWAFAANRSLAIASTAAVVRTSESPHLRVG
                     PRLRELLAHREVLVNLIRKELKVKYTASVLGAVWSLLNPIVFLAVFSFVVEVFSNPVP
                     DFPVFLLSGLLAWNFLANALQAGSRSVIDNANLVKKMAFPREILPLSSIGVALFDFLL
                     QSAVLLLIIGVWWAAGPGPGFHLPELALYPLAIVVLLVFTASLTFWVAALNVRYRDVG
                     HLLNLALLVWFWATPIVYQGWLVQRKLETISFIGLDGWTLYLLNPVTVIVLGFQRALY
                     SIVRPLDGGDPILPIISLGRLAGLLLAVLAVSTVVLYLAWGSFFRRSGDFAEEL"
     gene            4624..>5633
                     /locus_tag="VF036_02415"
     CDS             4624..>5633
                     /locus_tag="VF036_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HEX6264114.1"
                     /translation="MTTAIRIDGLSKMFRLFRERPTSLKQRVLVSRSRSEEFWALRDV
                     SFDIAEGSSMGLIGHNGSGKTTLLKCIAGILRPTAGTIRYQGRLAALLELGAGFHPDL
                     TGRENVYLNASFLGLSRRDTDRIFDDVVAFAELEDFMDNQVKFYSSGMLVRLGFAVAV
                     HVDPEVLLIDEVLAVGDESFQRRCLDRIRRFQADGRTIVLVTHALDVVRDVCDEAVML
                     EHGLLHAIGTPDDVVRTMRHRMLSDDPTFVPEQGSREVEISGVAIESEGGLRDTVRPG
                     EGLTITVDVRADAPVPDAAVSFAIVSGSTNHPVLEGKTSELGVSLGPVDRKKRGRFRI
                     PAA"
BASE COUNT          790 a         1929 c         1998 g          916 t
ORIGIN      
        1 ggcgacctcg ccgcgcggct cgccgaagcg gaggaggtca tgcggaaggc gcgaggtgac
       61 tacgagcggc tcgagatgga gctccgtgcc ctctcaccct ccggagcggc gaccacgggg
      121 agcgccgtgt ggacggagat cccggacggc tactactggg ccttcgagcg acgcatgcgc
      181 ggggctgcgg gatcgatcga ggatcgcctc cgcggctacg aggacctggt cgttccgcac
      241 cgcgaggccg tcgggacgac cgacgaccgc cgtccccgtt gggtcgatct cggctgcggg
      301 cagggcgagt tctgcgcctt gctcggcgag tggggctggg acgcggaggg cgtcgacgcg
      361 tctccggacg ccgtcgaggc gtgccgggcg cgcgggttcg acgtgacgct cgccgacgtc
      421 ctcaactatc tcgagacgcg gccggacgac ccggtcggcg gcgtcagcgc gatccagctg
      481 atcgagcatc tcccgaggcg ggcctgggtt cacctcttcc aggagatcca ccgtgcgctc
      541 gcgcccggcg gggcgctcct cctcgagacg atcaacggcc agaatcccga cgcgatcgct
      601 gactacttcg tcgcagacgt cacccatacg tggccggggc acccggaaac gctgcggctg
      661 atggccgagc acgcaggctt cgacgaggtc caggtgcggt tcctccacga ggaccatcgc
      721 gggcgagcgc aagacgtggc gatctgggcg gtcaaggcgt cctcgtgatc gacccgaggg
      781 acgggaagga agacgcgccg ggcggctcga cgcccgccga gccggctccc accgcccccg
      841 agaaccagga cgacgcggtc gcgagcctcg cgcgagaggt ctactgggca ggggaagggg
      901 gctcctcggg gcttcccgcg gacgctcgca ctctccgcga acgactggat gcgttcacgt
      961 accagatgcg cgccgaccag cgacaggccg ttcccgtcgg cgagacgttc ctcggctcgg
     1021 attccggctg gaagcgcggg ctgaagcagt tcgtgtggcg cctcactcgg ttctcgacga
     1081 tgcgctacga ccggctcctc gcggagctcg ccgagatgaa cggcgagctc gcacggcgcc
     1141 tcgtggcgac cgaggaggag ctctcccgcc tgcgacgtcg gctggatcat cgcgacgggg
     1201 atgcccgatg agggtcgccg tcctgcatcc gcagacggcg ttcacgcgag gtggcgccga
     1261 gctccacacg gaggcgctgg tgcgcgccct ccgggagtcg ggacacgacg ccgaggaggt
     1321 gacgatcgcg ggcaagtggt atccggcgac cgagctcgcc catcagatgg ccgtgtggcg
     1381 gagcttcgac atctccgaat cgaacggcct gaaggtggac gccgtgatcg ccctgaagtt
     1441 ccccgcctac ctcgcggccc acgagcggaa gatcgtgtgg ctgatccatc agcaccgctc
     1501 ggcctacgag ctctgggacc atccggactt cgccgacctc tcccggcagg acgagggtcc
     1561 tttgatccgc cggctggtgt ggaactcgga ccggctcggg ctgggcgaag ccaagcaggt
     1621 cttcacgaat tcgaagaacg tcgcggagcg tctgtggaca tcgctggggc tgaagggcga
     1681 cgtgctgtac caccgatcgc cggcgacgga ggcactgctc gcgcgcgatc ccgaggagtt
     1741 gggccactac ctgttctatc ccagccggct ggagatcctg aagcggcagt cgctcgcgat
     1801 cgatgcgatg cggcacgtga cgacgccggt gaagctcgtc ctcgtggggc ggggccccga
     1861 cgagcgctcg ctccgggagc ggatcgccgc gcacggtctc gaggaccggg ttcggctcga
     1921 ggtgggcgtc tccgatgaac ggctgcacga gctctaccga ggggcgctgg gcgtctactt
     1981 cgggccgttc gacgaggact tcgggtacgt cacgaccgag gggttcgccg ccgcacggcc
     2041 ggtcgtcacg ctcacggaca gcggggggcc gctcgagttc gttcgtcacg aggagtcggg
     2101 gctcgtcgcg aggcccgagc cgaaggcgat cgccgcggcg ttcgaccggc tctggtccga
     2161 tcgagatgcg gcgcgtgcgt ggggcctcga ggggcaccgc ctcgtgcgcg agacggtgcc
     2221 ggagtggccc gcggtcgtcg cgcggctact cggatgaagc cgacccgcag gctcgcggga
     2281 cgggtggcgc gacgggtcgg gttgcggcgc ccggctgggt cgggccgcag gacgatcgcc
     2341 cgccggacga acggccggct ggcgttcgtg ggtccgatgc cgccggcatc caccgggatc
     2401 gcgtcgtacg accaggcggt gatggacgga ctccggcgca ccgggttcct gcatcggcat
     2461 cgaaccgacg tgatctggcc ggtgcggtcc tcgcaggacc gcaggatcgc cgcctacgac
     2521 ctcgggatct tccagttggg gaacaacgcg aggttccacg ggcagatcta ccggttggcg
     2581 atcgcgtcgc ctggtctggt cgttctgcac gacctcgccc tcgacgggct cgtcctcggc
     2641 ctcgcggcgc agggcgaccc ggcggcgatc gcgaccgcgc gcgaggccgc cgccctccgc
     2701 tcgcgggtca cggaccccga catcgcgatg cacgagccgt tgcgatcggt gtggtgcgcg
     2761 gccgtcgcca gagcgtcccg cggcatcgtg gtccactctc cgttcgggaa gcgctacctc
     2821 gagtccgtgg gtgtgcgcac tccgatcttc acggtgcccc atccggtcgt cgaggacgag
     2881 accacgatgc ggcgcgccga gccccgcgcg aaggagctgc gcgcgatgct cgcggcgcag
     2941 ggtgcgcgca cgatcgtcgt cgcccccggc gacgtgaacg aggcgaagtg cctccccgcg
     3001 ctcgtcgagg cgatcgccac cctgcccggc gacgtgcacg ccgcgctcgt gggccgtcgg
     3061 atcccgggga ccgacatcac cgcggcgatc gaggcctacg gcatcgggga tcgggtcacc
     3121 gtgcaccatg acgtctccga cgacgacttc cgggcctggc tcgtggccgc cgatatcgcg
     3181 gtggacctcc ggttcccgca tcgcggtgag gtgagcgggt cgctctcgat ggcgatggtg
     3241 gccggcaccc ccacgatcgt ttcggccacc ggcacgtacc tcgacatccc ggaggagctc
     3301 gtggtgcgcg tcgctccggg tccggccgat cccgcagagc tcgcgcgccg gatcctcgag
     3361 ctccgggacg aaccggagcg acgcgcgcgg gtcggctccg ccgcgcgcgc ccacatggct
     3421 gcgctccgcg acacggaggc gaccgcccga ggctacgagg aggcgatcgt cgcgacgcgc
     3481 gcgatcgtcg cccaccccac caggccgctg cgcgcccgat gggcggcgag cctggtcgcg
     3541 gtggggatcg acgacaccct cgtggaggag ggctacggcg tctcctatgt gcgggcgctc
     3601 gaaagcttcg aacgaacgcc atgatcgttc cgcggtgcgg cgcagggcca ttgctagact
     3661 cgcgaccgtc gccctgacct gggcgttcgc cgcgaacagg agcctcgcca tcgcctcgac
     3721 ggccgccgtc gtccgcacgt ccgagtcgcc ccacctccgc gtggggccgc ggctgcgcga
     3781 gctgctcgcg catcgcgagg tgctcgtcaa cctgatccgc aaggagctga aggtgaagta
     3841 cacggcgtcg gtactgggcg cggtgtggtc gctcctgaac ccgatcgtct tccttgcggt
     3901 cttctccttc gtcgtcgaag tcttcagcaa cccggtcccc gacttccccg tgttcctgct
     3961 gtcggggctc ctcgcctgga acttcctcgc gaacgcgctt caggcaggct cgcggtccgt
     4021 gatcgacaac gccaacctcg tgaagaagat ggccttcccg cgggagatcc ttccactctc
     4081 gtcgatcggt gtcgcgctgt tcgacttcct gctccagtcc gcggtcttgt tgctgatcat
     4141 cggggtgtgg tgggccgcgg ggccgggacc cggcttccac ctgccggagt tggccctgta
     4201 tccgctggcg atcgtggtgc ttctcgtgtt cactgcatcg ctcacgttct gggtcgcggc
     4261 gctcaacgtg cgctaccgcg acgttgggca cctcctgaac ctcgccctgc tcgtctggtt
     4321 ctgggcgacg ccgatcgtgt accaggggtg gctcgtccag cggaagctcg agacgatctc
     4381 cttcatcggt ctcgacggct ggaccctcta tctgctgaat ccggtcacgg tgatcgttct
     4441 cgggttccag cgcgcgctct actcgatcgt gcgaccactc gacggcgggg atccgatcct
     4501 cccgatcatc agcctcggaa ggttggcggg gctgctactc gcggtcctgg ccgtctcgac
     4561 cgtcgtgctc tacctggcgt ggggttcctt cttccgtcgc tcgggcgatt tcgcggagga
     4621 gctgtgacga ccgcgatccg gatcgacggc ctctccaaga tgttccggct cttccgcgaa
     4681 cgtccgacgt cgctcaagca gcgggtcctc gtctcgcgat cacgctcgga ggagttctgg
     4741 gcgctccgcg acgtgtcgtt cgacatcgcg gagggatcct cgatgggcct gatcggtcac
     4801 aacgggtcgg ggaagacgac gttgctcaag tgcatcgccg ggatcctccg tcccacagcc
     4861 ggcacgatcc gctaccaggg gcgcctggca gcgctcctgg agctcggcgc cggcttccat
     4921 cccgatctca ccggcaggga gaacgtgtac ttgaacgcat cgttcctcgg cttgtcacgg
     4981 cgagacaccg accggatctt cgacgacgtc gtggcgttcg ccgagctgga ggacttcatg
     5041 gacaaccagg tgaagttcta ctcctcgggg atgctggtgc ggctcggctt cgccgtcgcc
     5101 gtccacgtgg atccggaggt gctcctgatc gacgaggtgc tggcggtagg cgacgagtcc
     5161 ttccagcgac gctgcctcga tcgcatccgc aggttccagg cggacggccg cacgatcgtg
     5221 ctggtcacac acgcgctcga cgtcgtccgc gacgtctgcg acgaagcggt gatgctggag
     5281 cacggcttgc tgcacgcgat cgggacgccg gacgacgtcg tccgcacgat gcggcatcgg
     5341 atgctgagcg acgacccgac gttcgtcccg gaacagggga gccgcgaggt agagatctcc
     5401 ggcgtcgcga tcgagtccga gggaggcctc cgcgataccg tgcgtccggg cgaagggctg
     5461 acgatcacgg tcgatgtccg tgccgacgca cccgtccccg acgcggccgt ctcgttcgcg
     5521 atcgtctcgg gatcgaccaa ccaccccgtc ctcgagggga agacgtccga gctcggggtc
     5581 agtctcggcc cggtcgaccg caagaagcgg ggccggttcc ggatccctgc ggc
//
LOCUS       DASUVE010000117         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_812141, whole genome shotgun sequence.
ACCESSION   DASUVE010000117 DASUVE010000000
VERSION     DASUVE010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812141"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VF036_02420"
     CDS             complement(<1..985)
                     /locus_tag="VF036_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6264115.1"
                     /translation="MSEPGRTMPPPPLRRRPQLLTYPDSLGGNLQSLASLLDGTLDGL
                     FEGVHILPPFPSSGDRGFAPLTYSEIDPQFGSWADIERIARRHDVLLDVMVNHVSRAS
                     PEFRDFLRRGRRSPHADLFITLDKVWPDGEPPAEDVARIFLRKPNAPFSTITVEETGE
                     RERIWTSFGTADWSEQIDLDLTSTATRSLITDWLTSLASHGVRIVRLDAVGYVIKKPG
                     TTCFMVEPEIYDFLDWVTAVAGTVGLVVLPEVHDSYATHERLSAHGYWTYDFVLPALL
                     LSAFHSGSTESLRSHLARSPERQFTMLDCHDGIPVRPDLDGILEPDEMRALA"
     gene            complement(982..2193)
                     /locus_tag="VF036_02425"
     CDS             complement(982..2193)
                     /locus_tag="VF036_02425"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6264116.1"
                     /translation="MSTHAFDEIELEDGRRIDGFVEEGFGGVMDAFVANFAERQDLGA
                     GCAVYADGRPVVDVWGGIADRRSGTEWTHKTTAVVFSCSKGLLTICVYRLADEGKLDV
                     DAPIVRYWPEFGRSGKSGITIRDALTHRAGLPALDVDLTKDQVLAWDPVIRAIEAQPP
                     MYARTDGHFYHAMTYGWLVGEVIRRITGLTPGSFFRQSLGDRLGLRTWIGLPAEEQAR
                     VAWMEAPLPDEDSDAARENARVAKDNALVDRSLTMSGAFAFPAEDEYVTFNDPAIRAG
                     EIPGANGISTAESLARLYGACVSDIDGAPILSASSLADACRPRASGPQLSGLPDDGAR
                     WGTGFQLASPPNQPMLGRGSFGHAGAGGQLAFGDVDHRIGFAYLSNQMGGYGDVRARA
                     LSGAVKTALGA"
     gene            complement(2190..>2317)
                     /locus_tag="VF036_02430"
     CDS             complement(2190..>2317)
                     /locus_tag="VF036_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6264117.1"
                     /translation="YATDIPLLCAGTILILLPTLVLFIVFQRQVIAALLQGSVKG"
BASE COUNT          411 a          745 c          783 g          378 t
ORIGIN      
        1 tcgccagggc tcgcatctcg tcgggctcga ggatgccatc gagatctggc cgcacaggga
       61 taccgtcgtg gcagtcaagc atcgtgaact gacgttccgg cgatcgtgcc agatgcgacc
      121 ggagggattc ggtcgatccc gagtggaacg ccgagaggag cagggccggc agcacgaagt
      181 cgtaggtcca atacccgtgc gccgaaagcc gctcgtgggt cgcgtagctg tcgtgaacct
      241 ccggcagcac gacgagcccg accgtcccgg ccaccgcggt cacccagtcg aggaaatcgt
      301 agatttccgg ctcgaccatg aagcaggtgg tgccgggctt cttgatcacg tagccgaccg
      361 cgtccagacg gacgatccgg acaccgtgcg acgcgaggga cgtaagccag tctgtgatca
      421 acgaccgcgt tgcggtcgag gtcaggtcca ggtcgatctg ctccgaccag tcggctgtcc
      481 cgaacgacgt ccagatccgt tcccgctcgc cggtttcctc gaccgtgatg gtcgaaaagg
      541 gcgcgttcgg cttgcgcaga aagatccgcg cgacatcctc ggcgggcggt tcgccgtccg
      601 gccagacctt gtcgagcgtg atgaacaggt ccgcatgagg agaccgtcgt ccccgtcgga
      661 gaaagtcgcg aaactccggg ctcgcgcggg agacgtggtt gaccatcacg tcgagcagca
      721 catcgtggcg ccgggcaatc cgctcgatat cggcccacga cccgaactgc gggtcgatct
      781 cgctgtaggt cagtggcgcg aagccccggt cgccggacga tggaaacggc gggaggatgt
      841 ggacgccttc gaacaggcca tcgagcgtgc catcgaggag cgacgccagc gactggaggt
      901 ttccgcccag cgaatcggga tatgtgagca gctgcggacg ccgtctcagc gggggcggtg
      961 gcatcgtcct tccgggttcg ctcatgcgcc gagggcggtc ttcacggctc cgctgagcgc
     1021 ccgtgcccgc acgtcgccgt atccgcccat ctgattgctc aggtaggcga acccgattcg
     1081 atgatcgacg tcgccgaagg cgagctgacc accggctccg gcgtgcccga agctgccgcg
     1141 accgagcatt ggctggttcg gcggtgaggc aagttggaag ccagttcccc agcgagcgcc
     1201 gtcatcgggg agccctgaca gttgaggccc actcgcgcgg gggcgacagg catcggcgag
     1261 cgacgacgcg gataggatcg gcgcgccgtc gatgtcggag acacacgccc cgtacaggcg
     1321 agcgagcgac tcggcggtgc tgatcccgtt tgcacccggg atctcacctg cgcggatcgc
     1381 gggatcgttg aaggtcacgt actcatcctc ggcgggaaag gcgaacgctc cgctcatggt
     1441 gaggctccga tccacgaggg cgttgtcctt cgcgacgcgg gcgttctcac gggcggcatc
     1501 ggagtcctcg tccgggagcg gtgcctccat ccaggcgacc cgcgcctgtt cctcggccgg
     1561 gaggccgatc caggttcgca ggccaagtcg gtcaccgagc gattggcgga agaagctgcc
     1621 cggtgtcagg ccggtgatcc gccggatcac ctcgccgacg agccagccgt aggtcatcgc
     1681 gtggtagaaa tgcccgtctg tccgggcgta catgggcggc tgcgcctcga tggcacggat
     1741 gacgggatcc caggcgagga cctggtcttt cgtcaggtcg acgtcgaggg cgggaagccc
     1801 ggcacgatgg gtcaacgcgt cacggatcgt gatcccggac ttgccgctgc ggccaaactc
     1861 cggccagtac cggacgatgg gtgcgtcgac gtcgagcttc ccctcgtcgg cgaggcgata
     1921 gacgcagatt gtgagcagcc ccttcgaaca tgagaagacc acggccgtcg tcttgtgcgt
     1981 ccattccgtc cctgaccgcc gatcggcgat gccgccccac acgtccacga ccggccggcc
     2041 atcggcgtag acggcacagc cggcaccaag atcctgacgc tctgcgaagt tggccacgaa
     2101 cgcgtccatg acgcctccaa agccctcttc aacgaatcca tcgatccgcc gcccgtcttc
     2161 gagctcaatc tcgtcgaaag catgtgtgct catcccttga ccgatccttg caggagggca
     2221 gcgatgacct gcctttggaa gacgatgaag aggacgaggg tcggcaacag gatgaggatc
     2281 gtccccgcgc acagcagcgg gatgtcggtc gcgtaat
//
LOCUS       DASUVE010000118         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1001564, whole genome shotgun sequence.
ACCESSION   DASUVE010000118 DASUVE010000000
VERSION     DASUVE010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001564"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..865)
                     /locus_tag="VF036_02435"
     CDS             complement(86..865)
                     /locus_tag="VF036_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6264118.1"
                     /translation="MAVAVVSTVVFFGIIVVAVLNSSGWPLIKEAFFDGEIFAEAFPK
                     IVEKFWRNVFIFVVAEVFVLIVAMIVAVLRSLRGPVFFPLRTLAAAYADLFRAIPTIL
                     VIFLLGFGAPALQIRGVPNSDVFWAIVALVLVYSAYVAEVYRAGIESVHPSQVASARA
                     LGLSRRKTLRFVVLPQAVRRVIPPLLNDFIGLQKDSALVVVLGYQEGLRTAQIIQSEN
                     FDFTPYVALALVYIVITIPQARFVDWLVAKDQRKRQTGGTL"
     gene            complement(989..1921)
                     /locus_tag="VF036_02440"
     CDS             complement(989..1921)
                     /locus_tag="VF036_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6264119.1"
                     /translation="MRKVLGLIVTSGLLLAACAEDDPTVGATGGAGETGETGATGATA
                     AATAADCAAANEAAFLTAGALTVGTGNPAFPPWWEGGESDDSEFEFNDPAKGEGYEGA
                     VVAEVATRLGFTFPDQVTFVPVGFNRSFAPGAKPFDFVLQQISYLPERDEAVDFSESY
                     YDVNQALVSTQGSPIDGATTVAELADATLGAPIGTTSLAVIEEVIQPSAEPQVYDDLA
                     AAVQDLKSGAIEGIVVDLPTAFFITAVQIPKGVVVGQFQTEGSQEYFAMAFETGSPLV
                     ECVNLALQEIKDDGTLDAIHEEWLAGNTNAPFLT"
     gene            complement(2017..>2276)
                     /locus_tag="VF036_02445"
     CDS             complement(2017..>2276)
                     /locus_tag="VF036_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6264120.1"
                     /translation="PAIVNAIILPLLFLSGIFIPLGPTAPEWMKTIGDIFPVKHFAEA
                     MLASFHGGDLRFEWSDVVIVALWGVAGLLAAIRFFSWEPRR"
BASE COUNT          398 a          796 c          747 g          335 t
ORIGIN      
        1 tcaacctcga ggtcgatccc gcgcagcacg tggaggctgt cgaacgactt gtgcagcccc
       61 tcgacgcgca gcgcgggatc ctggctcaca gcgtccctcc cgtctgacgc ttgcgctggt
      121 ccttcgcgac gagccagtcc acgaagcgtg cctgcgggat cgtgatcacg atgtacacca
      181 gcgcgagcgc gacgtacggc gtgaagtcga agttctccga ctggatgatc tgcgccgtcc
      241 tcagcccctc ctgatagccg agcaccacga cgagcgccga gtccttctgc aggccgatga
      301 agtcgttcag gagcggtggt atcacgcgtc gtacggcctg cgggagcacg acgaaccgca
      361 gcgtcttccg acgggacagg cccaaagcgc gcgccgaggc cacctgcgac gggtgcacgg
      421 actcgatgcc ggctcggtac acctcggcca cgtacgccga gtacacgagc acgagcgcca
      481 cgatcgccca gaacacgtcg gagttcggca cccccctgat ctgaagggcc ggcgccccga
      541 agccgagcag gaagatcacg aggatcgtcg ggatcgcgcg gaacaggtcc gcgtacgcgg
      601 cggcgagggt acggagtggg aagaacaccg gtccccggag actccggagg acggccacga
      661 tcatcgccac gatcagcacg aagacctcgg cgacgacgaa gatgaagacg ttccgccaga
      721 acttctcgac gatcttcggg aacgcctccg cgaagatctc tccgtcgaag aacgcctcct
      781 tgatgagcgg ccacccggag gagttcagga ccgccacgac gatgatcccg aagaagacga
      841 cggtgctgac gacggcgacc gccacgccgc gcgcaccctc tccccggagt ggcgcgagga
      901 tcttcgaccc tcggtcccct tcggggagag gatcctccgg tcgaagccga taggcgtccg
      961 gtgccggccc gctcaccgcg ccgggacgct acgtcaggaa cggggcgttg gtgttgcccg
     1021 ccagccactc ctcgtggatc gcgtccagcg tcccgtcgtc cttgatctct tggagcgcca
     1081 ggttcacgca ctcgacgagc gggctccccg tctcgaaggc catcgcgaag tactcctgcg
     1141 agccctcggt ctggaactgg ccgacgacca cgcccttcgg gatctggacc gcggtgatga
     1201 agaacgcggt gggcaggtcg acgacgatcc cctcgatcgc tccgctcttc aggtcctgca
     1261 ccgcagccgc gaggtcgtcg tagacctgtg gttcggcgct cggctggata acttcctcga
     1321 tcacggccag gctcgtggtc ccgatcggag ccccgagcgt cgcgtccgcg agctcggcga
     1381 ccgtcgtggc accgtcgatc ggcgacccct gtgtggagac gagtgcctgg ttgacgtcgt
     1441 agtagctctc gctgaagtcc acggcctcgt cgcgttcggg cagataggag atctgctgca
     1501 agacgaagtc gaacggcttg gcccccgggg cgaacgacct gttgaacccg accggcacga
     1561 acgtcacctg gtccgggaac gtgaagccga gtcgcgtcgc cacctcggcc acgaccgcgc
     1621 cctcgtagcc ctcgcccttg gccgggtcgt tgaactcgaa ctccgagtcg tcgctctcgc
     1681 ccccctccca ccagggcggg aaggcggggt tcccggtgcc gacggtcagg gccccggcgg
     1741 tgaggaacgc ggcctcgttc gccgcggcgc agtccgccgc cgtcgccgca gcggtcgctc
     1801 ccgtcgcgcc ggtctccccg gtctcaccag cgccaccggt ggcgccgacg gtcgggtcgt
     1861 cctccgcgca ggccgcgagg agcagaccgg aggtgacgat gagtccgagc actttccgca
     1921 tgtgagggaa cccccctgac cgaggcggag cagcgtcgcc gaatccgcgg agcctagcac
     1981 acgccctctt ctacgaagac gcgagcgctg ccggtctcag cggcgcggct cccacgagaa
     2041 gaacctgatc gcggcgagca acccggccac tccccagagc gccacgatga cgacgtccga
     2101 ccactcgaac cgcaggtccc cgccgtggaa tgaggcgagc atcgcctccg cgaaatgctt
     2161 cacggggaag atgtccccga tcgtcttcat ccattcaggg gcagtcggac caagggggat
     2221 gaagatcccg gacaggaaca ggagcggcag gatgatcgcg ttcacgatcg cgggcg
//
LOCUS       DASUVE010000119         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1567062, whole genome shotgun sequence.
ACCESSION   DASUVE010000119 DASUVE010000000
VERSION     DASUVE010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567062"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VF036_02450"
     CDS             complement(<1..215)
                     /locus_tag="VF036_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264121.1"
                     /translation="MHTMRRVGLVLIGCVLASLTLLATLILWLGPALGIACGLGAATV
                     VLTGYITVIGPWQRRWGASDEDIRRPMP"
     gene            complement(456..1238)
                     /locus_tag="VF036_02455"
     CDS             complement(456..1238)
                     /locus_tag="VF036_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HEX6264122.1"
                     /translation="MRTEPVTSSGALLFARYAHPPNLLGYCGPTDHLSFFEQATEGVV
                     DRGLGELTQAFDGAWPYLELIASANGIRDPLDRAVVHAYWIGNPLLERVDMSALGDSI
                     EERFRRRVGRGWDHLSTTILAGAVPHHTFHVLLVSPWTGLMRAGHPDHPLHVMDRCRI
                     RWGRVIEVVGDAASVAVSTLRWDGRELGIDSTGTELATVKTDGRGFLPDLHPGDHVSL
                     HWNWVCDRLSPRDVRSLDRYTTRHLSLGNQGNRRSSDAADLW"
     gene            complement(1238..>2130)
                     /locus_tag="VF036_02460"
     CDS             complement(1238..>2130)
                     /locus_tag="VF036_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6264123.1"
                     /translation="LAFATALVSGVAVFANGYGVTAFGSGGGVYTTAKNLVAAIFLLV
                     LTGTATMARSDEGFTRPRTRAQWAGLAGIAVIGGSVPFLLFFEGLTRASSLQAAFLHK
                     TLLIWVALLAWPLLRERIGPIHVLAIGLLLAGQAALQGGVGDVVIGTGEVMILAATLL
                     WSVEVIIAKRLLGSLSSLTVGTSRMALGCVVLVTYLASTGQIDDLLAASATQWAWATL
                     TGGLLAAYVATWYLALARARAIDVTAVLVFGAVVTTLLTVGVQGAEPQAAALALLVLG
                     TVIAVVAARRRAMSEAARTG"
BASE COUNT          377 a          743 c          706 g          304 t
ORIGIN      
        1 ggcatcggac gtcggatgtc ctcgtcgctc gccccccatc ggcgctgcca agggccgatg
       61 acggtgatgt accccgtgag gacgacggtc gccgcgccaa ggccacaggc gatacccaga
      121 gccgggccaa gccagaggat cagtgtggcg agcagggtca agctcgcgag cacgcagccg
      181 atcaagacca ggccgaccct tcgcatcgtg tgcatgccga tcttcccccc gcgggtatcc
      241 tccgttcgaa ctcaccttct tcgttttgac atgtcgttcc aggggccgaa ggacccgtcg
      301 cgtacggacc gtccggcatc aggacacttc gttccagaca tacaggcgtc ctcgcgacgg
      361 tgccggccac gatcgagctc gaggaggcag cccctggtca gagcgctgca cggcctcgaa
      421 catggaagag gtccgaggtt ctgatcctct ggcgcctacc agagatccgc tgcgtcggag
      481 ctgcgcctgt tcccttggtt cccgagggag aggtgccggg tggtgtagcg atcgagggac
      541 cggacgtccc tcgggctcag ccgatcgcac acccagttcc aatggagcga cacgtggtcg
      601 ccagggtgca ggtcagggag gaaccctcgc ccatctgtct tcacggtagc gagctccgtg
      661 ccagtggagt cgatccccag ctcgcggccg tcccatcgga gcgtggatac ggcgaccgag
      721 gccgcgtcac ccaccacctc gatcacgcga ccccatcgga tccggcatcg atccatcacg
      781 tggagcggat ggtcgggatg gccggcacgc atcagtccgg tccatggaga gaccagcagg
      841 acgtggaagg tgtgatgcgg cacggccccc gccaggatcg tcgtcgataa gtggtcccag
      901 cctcgaccca cgcgccgtcg gaagcgctcc tcgatcgagt ccccgagcgc cgacatgtcg
      961 acccgctcga gcagcgggtt cccgatccag tacgcgtgaa ccacggcgcg atcgagcgga
     1021 tcgcggatcc cgttggcgga cgcgatcagc tcgaggtaag gccacgcgcc gtcgaacgct
     1081 tgtgtcagct ctccgagccc gcgatcgacg acgccttcgg tggcctgttc gaagaacgag
     1141 aggtggtcgg tgggcccgca gtagccgagc aggttcggtg gatgcgcgta ccgagcgaag
     1201 agcagcgccc cgctactggt caccggctcc gtccgcatca gcccgtccgc gcggcttccg
     1261 acatggctcg ccgcctcgcg gccaccaccg cgatcaccgt tcccaacacg agcagcgcca
     1321 gcgcggccgc ctgcggctcg gctccctgta cgccgacggt caggagcgtt gtcacgacgg
     1381 ctccgaacac gagaacggcc gtcacgtcga tcgcccgcgc cctcgcgagc gcgaggtacc
     1441 aggtcgcgac gtacgccgca agaaggccgc cggtcagtgt cgcccacgcc cattgcgtgg
     1501 ccgacgcggc gagcagatcg tcgatctgtc cggtcgatgc gagatacgtg accaggacga
     1561 cgcagccgag ggccatccgc gaggttccga cggtgagcga cgacaacgag ccgaggagtc
     1621 gcttggcgat gatcacctcg accgaccaca acagcgtcgc cgccaggatc atcacctcgc
     1681 cggtgccgat gacgacatcg ccgaccccgc cctggagcgc cgcttgaccc gcgaggagaa
     1741 ggccgatcgc caggacgtgg atcggtccga tccgttcacg gaggagcggc caggcgagga
     1801 gcgcgaccca gatcagcagc gtcttatgga ggaacgccgc ctgcagcgac gaggcacggg
     1861 tcaatccttc gaagaacagc aggaacggca cactgccgcc gatcaccgcg atgccggcca
     1921 ggcctgccca ctgcgcgcgg gtccgtggcc gcgtgaagcc ctcgtcggag cgggccatcg
     1981 tcgccgtccc ggtgagcacg agcaagaaga tcgcggccac gaggttcttc gccgtcgtgt
     2041 agacgccgcc tcccgagccg aacgccgtca cgccgtagcc gttcgcgaag accgcgacgc
     2101 cgctgacgag cgccgtggcg aacgcgagcg
//
LOCUS       DASUVE010000120         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1002428, whole genome shotgun sequence.
ACCESSION   DASUVE010000120 DASUVE010000000
VERSION     DASUVE010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002428"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VF036_02465"
     CDS             <1..977
                     /locus_tag="VF036_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264124.1"
                     /translation="GPQIQGQFPFANGISTLGFGWLGAYFVLSGSPVAATAISLYGAG
                     TLTRLQTFTMLSGSRLGASFIVLLTGFLYAARNRNDPNRNEPIGMGIQAMTMTALVYL
                     PGMVIGYGIIRMGWLDGLNLHASGQLEAALSVVWGPVVDTVERIVPDTLLFVVGLLVI
                     LASFKVLDRVLPQVSSDSTAKRATWMRRPWTMFALGCIVATLTLSVSVALTVLIPLAV
                     KGYVRRDDALPYIMGANITTLADTLVVAMLQRDPVAAQIVLAEAIGVTIVSLAILAFW
                     YQPVKRRVIMIDDYFVGSTKRLAIFVGILFVLPVSFLLSGLWIGPILR"
     gene            1005..2327
                     /locus_tag="VF036_02470"
     CDS             1005..2327
                     /locus_tag="VF036_02470"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6264125.1"
                     /translation="MDDPTITARPSDAGPRASVSLLRRNRDFRALFFASVISLGGDWF
                     LWVAINGLIYEATGKALYVGLAILAQESAFFLASPIGGALADRIDRRKLMIACDLARA
                     VVCVSFLLVGPETIWLAYVLLPVLATFAAPFDPAFSAATPNVVDPDDLPAANALNGSL
                     WGTMLAVGAGLGGLVSAAFGDDTAFIVDAVSFLVSAALLSSIRRGFSESRAETTEHPS
                     IVEATRETWRFARRDHRVLSLLAVKFGFGAAAGLLALIPVMALDADVFDSGNVGFGIL
                     MAARGVGALIGPFLGHRIAGPGHRRLFPAIGLSLAVFGLGYVAMGQAPSLAIAAVMIC
                     VAHLGGGSQWMLSTYGLQVLVPDHIRGRIFGFDYMLITLSLSVSALIASAVADQIGAP
                     DTVTILGGVALVWALVWFVLSRGVRRTGLDGSTEPEPDTGSEPIPAIS"
BASE COUNT          386 a          950 c          965 g          513 t
ORIGIN      
        1 tcggaccgca gatccagggt cagttcccgt tcgcgaacgg gatctcgacg ctggggttcg
       61 gctggctcgg cgcctacttc gtgctgtcgg gctcgccggt cgcggccacg gcgatcagcc
      121 tgtacggagc ggggacgctg acgcggctcc agaccttcac gatgctctcg gggtcgcgcc
      181 tcggcgcatc gttcatcgtg ttgctgaccg ggttcctcta cgccgcgcgg aaccggaacg
      241 accccaaccg gaacgagccg atcgggatgg ggatccaggc gatgacgatg acggctctcg
      301 tctatctgcc cgggatggtc atcggctacg gcatcatccg gatggggtgg ctcgacgggt
      361 tgaacctgca cgcgtcgggg cagctcgagg ccgcgctgtc cgtcgtgtgg ggacccgtcg
      421 tcgacacggt cgagcggatc gtgccggaca cgctcctctt cgtggtcggg ctcctcgtga
      481 tcctggcgtc gttcaaggtg ctcgaccggg tcctcccaca ggtcagcagc gactcgacgg
      541 cgaagcgagc aacgtggatg cgtcggccct ggacgatgtt cgcgctgggc tgcatcgtgg
      601 cgacgctgac cctctcggtc tccgtcgccc tgaccgtgct tatcccgctc gcggtgaagg
      661 gctacgtccg ccgtgacgac gcgctcccgt acatcatggg cgcgaacatc acgaccctcg
      721 cggacacgct ggtcgtggcg atgttgcagc gcgatcccgt cgcggcgcag atcgtgctcg
      781 cggaagcgat cggcgtcacg atcgtgtcgc tggcgatcct cgcgttctgg taccagccgg
      841 tgaagcggcg ggtgatcatg atcgacgact acttcgtggg ctcgacgaag cggctggcga
      901 tcttcgtcgg catcctcttc gttttgccgg tgtcgttcct cctctcgggg ctctggatcg
      961 ggccgatcct gcgataggtc gcacctgtac gctggcgagc cgccgtggac gacccgacga
     1021 tcaccgcacg cccttcggac gccggtccgc gcgcgagcgt gtcgctgctc cgccgcaata
     1081 gggacttccg cgcgctgttc ttcgcctcgg tgatctcact aggcggcgac tggttcctct
     1141 gggtggcgat caacgggctg atctacgagg ccaccggcaa ggcgctgtac gtgggcctgg
     1201 cgatcctcgc gcaggagtcc gcgttcttcc tcgcctcacc gatcggaggg gcgctggcgg
     1261 atcggatcga ccgacggaaa ctgatgatcg cctgcgatct cgcccgggcg gtcgtctgtg
     1321 tgtccttcct gctggtcggg cccgagacga tctggctcgc gtacgtgctc ctgcccgtgc
     1381 tggcgacgtt cgccgcgccg ttcgatccgg cgttctcggc cgcgacgccg aacgtcgtcg
     1441 atcccgacga cctccccgcc gcgaacgcgc tgaacgggtc cctgtggggg acgatgctcg
     1501 ccgtcggcgc gggactcgga ggcctcgtca gcgcggcgtt cggcgacgac acggcgttca
     1561 tcgtcgacgc cgtgtccttc ctggtctccg cggcgttgct gtcgtcgatc cgacgcgggt
     1621 tctccgagtc ccgggccgag acgacggagc atccgagcat cgtcgaggcc acccgggaga
     1681 cctggcgctt cgctcgccgt gaccaccgcg tcctgtcgct gctggccgtg aagttcgggt
     1741 tcggcgccgc cgccggactc ctcgcgctga tcccggtcat ggcgctcgac gccgacgtgt
     1801 tcgactccgg caacgtcgga ttcgggatcc tgatggcggc acgcggggtg ggagccctga
     1861 tcgggccctt cctcgggcac cggatcgcgg gccccggaca tcggcggctg ttccccgcga
     1921 tcgggctctc gctcgcggtg ttcggcctcg ggtacgtggc gatggggcag gcgccctccc
     1981 tggcgatcgc cgccgtgatg atctgcgtcg cgcacctcgg cgggggatcc caatggatgc
     2041 tttcgacgta cggcctccag gtgctggttc cggatcacat cagagggcgg atcttcggct
     2101 tcgactacat gctgatcacg ctgtcgctct cggtctccgc gctcatcgcg tcggcggtgg
     2161 ccgatcagat cggcgcgccg gacacggtca cgatcctcgg cggcgtcgcg ctcgtgtggg
     2221 ccctcgtgtg gttcgtgctc agccgaggcg tccgcaggac cgggctcgac ggctccaccg
     2281 agccggagcc cgacaccggg tccgaaccga tccccgcgat cagctgacca cgatcggggc
     2341 gggggggggg cgccgtgtgt gccaccatcg ccacctcgga gaggatgagc gacatgcgaa
     2401 ggaacatccg tttcgcgctt ccagtggccg tggccatctc gatcgttgga acgaccgtgc
     2461 acgcgggcac accggcgaag gtcatcgaca cgaccggtta tgaattcgga tccgcatctg
     2521 gtgagggctg gatcgcctgg tccaagggcg tcagctacgg aggggatcac gacctgtacg
     2581 tgaagcacac cggccagccc gccgacgtcg tcgaggcagg gaagtatcag caggcgggca
     2641 acatcgagat cgcgggacca acggcgacat cctggagttc cacatcgaac ccagggtcgg
     2701 agacgcggag atccgcttct acgacctcgc gaccgagctc gtcagtgctc ctcccgccgg
     2761 gatcaacacc gcgaaggacg acgacttccc gtcgatctcc gggaaccacc tgtt
//
LOCUS       DASUVE010000121         3350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1567849, whole genome shotgun sequence.
ACCESSION   DASUVE010000121 DASUVE010000000
VERSION     DASUVE010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567849"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VF036_02475"
     CDS             complement(<1..187)
                     /locus_tag="VF036_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6264126.1"
                     /translation="MEHVRCSRSGGVRGPMSTQNTLRGRDRECAALDRLLDAVRGGES
                     RALVLRGEPGVGKSALLQ"
     gene            410..1234
                     /locus_tag="VF036_02480"
     CDS             410..1234
                     /locus_tag="VF036_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6264127.1"
                     /translation="MATSASDATPNTIVLIHGFWVTPRSWEHWKRHYEAKGYTVLAPA
                     YPGFEVEVEALRADPTPIEELEVPEIIDMLENMIGKLDRPPIIMGHSAGGVFTQILLD
                     HGFGAAGVAINSAPTEGVKVIPLSQIRSGFPVLKNPANRHRAVGFTPEHWHYVFANTF
                     DEQESQALYDRYHIPASGHVFWGSALANIHPGKDDTYVDYHNDSRPPLLFIVGKQDHL
                     FPPKVGHSNVKRYKSTSVTDIVEFDGPHLLPAIPGWEAVADYALDWALKHAAAPAS"
     gene            1298..2143
                     /locus_tag="VF036_02485"
     CDS             1298..2143
                     /locus_tag="VF036_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6264128.1"
                     /translation="MDTGKKSTHVPRRIVATSAVLLLVVLVFSGGGHVAAGLQKSSGP
                     RPTIVLVHGDWADASSWNAVIAPLQDRGFTVVAPPNTLRGPSQDAPYLASYLQTIPGP
                     IVLAAHSYGGFVITNAATGNPNVKALVYIDSFAPDVNETALDLVGPTDSCVNDDGAFR
                     AVPIDGGLDLYLNWEADPPYAGFRECFANGVDEQTARLLAAGQRPASAAQFSDPSGVP
                     AWETIPSWALLGTRDHVIPPALQAEMYERADARITRVRAGHLTPITRPGAVVRVILSA
                     VEATT"
     gene            2162..2929
                     /locus_tag="VF036_02490"
     CDS             2162..2929
                     /locus_tag="VF036_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6264129.1"
                     /translation="MKVVVIGGSGLIGSKLVEELREAGQDPLSASPDSGVDTLTGEGL
                     AEALENARVVVDVSNAPMWDDAAVLEFFQTSTRNVRAAEVGAGVSHHVALSVVGTDRL
                     QESGYFRAKLAQEEAIKAGSIPYSIVRATQFFEFLGRIADSSSDGETVRLPPVLIQPE
                     AADDVAAALAVAAVGAPVNGTIELAGPERFRFDELIQRVLDANGDGRRVMADEGARYF
                     GAELEEGSLVPGDDAHIAPTRFEDWIERIMVEQTRPT"
     gene            2952..>3350
                     /locus_tag="VF036_02495"
     CDS             2952..>3350
                     /locus_tag="VF036_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6264130.1"
                     /translation="MSATVSPLPITDLELEQVRQANASEATPVVFIHGLWLLPSSWAN
                     WAEFFNRAGYAPLTPVWPDDPRTVEEARANPDVLAKKTLRQIADHTTEVIEALDRKPV
                     IMGHSTGGLLAEMLAGQGLATVTVAIDPGVF"
BASE COUNT          572 a         1108 c         1139 g          531 t
ORIGIN      
        1 actgcagcag cgccgacttg cccacgccag gttcgccgcg gagcacgagg gcgcggctct
       61 cacccccacg gaccgcgtcg aggagtcgat caagagccgc gcactctcga tcgcgccctc
      121 gcagggtgtt ctgcgtggac atcggtcccc ggaccccccc gctgcgggag caccgcacgt
      181 gctccatcgc ggccgacgtg ccatcctggg cgtccccgcc tggctggcat tgacggagtg
      241 aacaacgtac accctgcgcc gcagcgccgg gacgcacgcg cggtgactcg gccgatgggg
      301 actggcgacc tcggcaggcc tgttggcgga ttcgaaccct gcctcggtcg ggaatcctcg
      361 ccgtgaccga cttccgttgg gcaggtatgg ggaaggaagg agaaggggca tggcgacatc
      421 ggcatcagac gcgactccca acacgatcgt gctgatccac ggcttctggg ttacgcctcg
      481 cagctgggag cactggaagc ggcactacga ggccaagggc tacacggtct tggcgcccgc
      541 atacccgggg ttcgaggtcg aggtcgaggc gctccgcgcc gatccaacac cgatcgagga
      601 actcgaggtg ccggagatca tcgacatgct cgagaacatg atcggcaagc tcgaccgtcc
      661 tccgatcatc atgggccact cggctggtgg ggtgttcacg cagatcttgc tcgaccatgg
      721 attcggcgcg gcgggggtgg cgatcaactc ggcgccgacg gaaggcgtca aagtgatccc
      781 gctctcgcag atcaggtccg ggttcccggt cctgaagaac ccggcgaacc gccatcgagc
      841 ggtcggcttc acaccggagc attggcacta cgtcttcgcg aacacgttcg atgagcagga
      901 gtcacaggcg ctctacgacc ggtaccacat ccccgcgtcg gggcacgtct tctggggaag
      961 cgcactcgcg aacatccacc ctggcaagga cgacacatac gtcgactacc acaacgattc
     1021 gcggccgccg ctgctcttca tcgtcgggaa gcaggatcac ctgttccctc cgaaggtcgg
     1081 gcattcgaac gtgaagcgtt acaagtccac gtcggtcacc gacatcgtgg agttcgacgg
     1141 accgcacctg ttgcctgcga tcccgggttg ggaggctgtc gcggactacg ccctcgactg
     1201 ggcgttgaag cacgccgcgg cgccggcttc ctgaacgcga cgggcgtggc gcgcgccacg
     1261 ttggctccga catccgtcag gaacaaggga gggatccatg gacaccggca agaagtcgac
     1321 gcacgttccc cgccgcatcg tcgcgacgag cgccgtcctt ctcctcgtcg tcctcgtttt
     1381 cagcgggggt gggcacgtcg cagccgggct gcagaagagt tcaggcccgc gaccgacgat
     1441 cgtgctcgtg cacggtgact gggcggacgc ttcgtcgtgg aacgcggtga tcgcgccgct
     1501 gcaagatcgc ggattcaccg tggtggctcc gcccaacacg ctgcgcggac cgagccagga
     1561 cgccccgtac ctcgccagct acctccagac gattcccggt ccgatcgtgc tcgcggcgca
     1621 ctcgtacggc ggcttcgtga tcaccaacgc ggccacgggc aaccccaacg tgaaggcgct
     1681 cgtgtacatc gattccttcg cccccgacgt gaacgagacc gccctcgatc ttgtgggacc
     1741 cacggactcc tgcgtcaacg acgatggcgc tttccgcgcg gtgccgatcg acggcgggtt
     1801 ggatctgtac ctgaattggg aagcggatcc cccgtacgct gggttccgcg aatgcttcgc
     1861 caacggcgtc gatgagcaga ccgccaggct cctcgccgcc gggcaacgac ccgcgtcggc
     1921 cgcgcagttc tccgatccgt ccggcgtacc cgcgtgggag acgatccctt cgtgggcgct
     1981 cctcgggacc cgggaccacg tgatcccgcc ggccctccag gctgagatgt acgagcgtgc
     2041 cgatgctcgg atcaccaggg tgagggccgg gcatctcacc ccgatcacgc ggccgggggc
     2101 cgtcgtgcgg gtgatccttt cggcggtcga ggcgaccaca taactcggaa ggaggcgggc
     2161 gatgaaggtc gtcgtgatcg gtgggagcgg gctcatcggg tccaagctcg tcgaggagct
     2221 ccgggaggcg gggcaggacc cactctcggc gtcgcctgac tcgggcgtcg acacgctcac
     2281 cggcgagggt ctggccgaag cgctcgagaa cgcccgggtc gtggtcgacg tgtcgaacgc
     2341 gccgatgtgg gacgacgctg cggtgctgga gttcttccag acgtcgaccc ggaacgtccg
     2401 cgccgcagag gtgggtgctg gcgtcagcca tcacgtggcg ctgtcggtcg tcggaaccga
     2461 tcggctccag gagagcggat acttccgggc gaagctcgcc caggaggaag cgatcaaggc
     2521 ggggtcgatc ccgtactcga ttgttcgcgc gacgcagttc ttcgagttct tgggccgaat
     2581 cgccgattcg agcagcgacg gcgaaacggt gcgcctgccg ccggtcctga tccagcccga
     2641 ggcagccgac gacgtcgccg ctgcgttggc ggtggccgcg gtcggcgcgc cggtgaacgg
     2701 gacgatcgaa ctggccggtc ctgaacggtt ccgtttcgac gagctcatcc aacgcgtgct
     2761 ggatgcgaac ggtgacggac gccgggtgat ggccgacgag ggtgcccgct acttcggggc
     2821 agagctcgag gaaggctccc tcgtcccggg cgacgacgcg cacatcgctc cgacccgctt
     2881 cgaggattgg atcgaacgga tcatggtcga gcagacgaga cccacataga tcgacagaca
     2941 gaggaggcca gatgagcgcg accgtgtccc cgttgccgat cacggacctc gagctcgagc
     3001 aggtccggca ggcgaacgcg agcgaggcaa cgccggtggt cttcatccat ggcctctggt
     3061 tgctgccgag cagctgggcc aactgggccg agttcttcaa ccgggccggc tacgctccgc
     3121 tgacgccggt ctggccagac gatccgagaa ccgttgaaga ggctcgggcg aacccggacg
     3181 tcctcgccaa gaagaccttg aggcagatcg ccgaccacac cactgaggtc atcgaggcct
     3241 tggacaggaa gccggtgatc atggggcact ccaccggggg cctgctggcg gagatgctcg
     3301 cgggacaagg cctcgccacg gtaacggtgg cgatcgaccc cggcgtcttc
//
LOCUS       DASUVE010000122         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1002538, whole genome shotgun sequence.
ACCESSION   DASUVE010000122 DASUVE010000000
VERSION     DASUVE010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002538"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..837
                     /locus_tag="VF036_02500"
     CDS             124..837
                     /locus_tag="VF036_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264131.1"
                     /translation="MTQQPPTAAPVRRSPTSRAEDSARRRTIARRRIAVAVGGVAVLA
                     IAAILLSGGTVPLIDAGPDGPEGFSFQLGKVQPTTISRTPRSELNDVAREAGAGVKET
                     MDELYFRAFVDQGSWGDYEAAFELFEGRAATRAGSDAEVLTLGSTANDDFEALDEASG
                     TLTVTVLTDAKDAPVSAVAQVEFEATVEARGGGTSTQVVSVGSFFLRQVEGAWRIFAY
                     EVDREDAEAAATSPSGSPS"
     gene            849..1769
                     /locus_tag="VF036_02505"
     CDS             849..1769
                     /locus_tag="VF036_02505"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX6264132.1"
                     /translation="MIATIATITAGVSGLGVATLHATHAVGAPVMQIGAAHAEYGPTI
                     TGNDPIFILILGSDSRPGTALQDGLCDSIHILGIDPKTKRATLVGIPRDSYVPIASGG
                     TNKINVALSQGGPEGMVATVENLTGITFDYYLLTGFEGMTRIFDALGGLRIDVPYSFK
                     GYENTDFTEGRTTMTGAETLEYSRTRKSLSHGDFDRSMNQGRILLAAFSQFRAEFRKD
                     PAALFQWIAAGMRNVSTDLPLDELLTLAFTSWEIRPARLTNLVAVGGVGTAGGASIVS
                     LPSPHPVFEDVAADGFILPQDIPADAEPAG"
     gene            1817..2491
                     /locus_tag="VF036_02510"
     CDS             1817..2491
                     /locus_tag="VF036_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264133.1"
                     /translation="MAKEQPLSRSPRRIPLIGAGVAVLLVIGVLVFLTREDAPTEGAG
                     GEETTSDGDVASPTTPPFGFDDTSRKMVKTSSEPMGSRHRQASVAAATEAEDVLTDLY
                     TDGFLDPANWERGSYAGAFGGFARGARDQAETHPGPLTAGPGAGDRYERIEPKSGRIA
                     TRILLDRQGAPVLLVSVVRFSAVATGSDEVVLRSVGQYFFERVGRAWRIVSFDVTRND
                     GPRKAA"
     gene            2488..>3051
                     /locus_tag="VF036_02515"
     CDS             2488..>3051
                     /locus_tag="VF036_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX6264134.1"
                     /translation="MTRRAAVAVVVLLAWVAGSTVGSIAELPAASARTPGIVFGRAHG
                     GYTPDPTGRRTITILAIGSDARPGQNPLALRADSIHVIFLHPRKQRAVVVGIPRDSFV
                     SIPGLGTTKINAAMSSGGPARMVQTVEQNFGARIDYWAVTSFWGITRMFETIGGLQVR
                     VPFRMKDRFSESNFHPGFQRLRGRQVLA"
BASE COUNT          461 a         1057 c         1068 g          465 t
ORIGIN      
        1 cgaccagtct tcccggcacg ccatcaggtg gaaccgacgc atcctatccc acacccgatc
       61 ctcgacgcgc gcttccgatg gtgattggaa cacctccgac cggcgtgtac cctcggtcgc
      121 gccttgacac agcaaccccc caccgccgcc cccgtgcggc gatcccccac gagccgcgcg
      181 gaggattccg cgcgtcgccg gacgatcgcc cgccgtcgta tcgctgtcgc cgtcggtggc
      241 gtcgcggtcc tcgcgatcgc cgcgatcctg ctgtcgggcg gcacggtgcc gctgatcgac
      301 gccggaccgg acgggccgga ggggttcagc ttccagctcg gcaaggtcca gccgaccacc
      361 atcagccgaa cgccgcgatc ggagctgaac gacgtggccc gggaagccgg agcgggcgtc
      421 aaggagacga tggacgagct ctacttccgg gcgttcgtcg accagggctc gtggggcgac
      481 tacgaggcgg cgttcgagct cttcgaggga cgcgcggcca cccgcgcagg atcggatgcc
      541 gaggtgctca cgctcggatc caccgcgaac gacgacttcg aggccctcga cgaggcgagc
      601 ggaaccctca cggtgaccgt gctcaccgat gcgaaggacg ctcccgtcag cgcggtcgcc
      661 caggtcgagt tcgaggcgac ggtggaggcc cggggcggcg gcacgtcgac gcaggtcgtg
      721 agcgtcggtt cgttcttcct tcgtcaggtg gagggcgcgt ggcggatctt cgcctacgag
      781 gtggaccgcg aagacgccga ggcagccgcc acgagtccgt cggggtcgcc ctcgtgatgc
      841 ggcgacggtt gatcgcgacg atcgcgacga tcacggctgg cgtctcagga ctcggggtag
      901 cgacactgca cgcgacgcac gccgtgggtg ctcccgtgat gcagatcggc gccgcccacg
      961 ccgagtacgg gccgacgatc accgggaacg atccgatctt catcctgatc ctcggcagcg
     1021 actcccggcc gggtaccgcg ctgcaggacg ggctctgcga ctcgatccac atcctcggga
     1081 tcgacccgaa gacgaaacgg gccaccctgg tggggatccc gcgtgattcc tacgtgccga
     1141 tcgcgagcgg agggacgaac aagatcaacg tcgcgctgtc ccagggcggt cccgagggga
     1201 tggtcgcgac ggtcgagaac ctcaccggca tcacgttcga ctactacctg ctcaccggct
     1261 tcgaagggat gacgcggatc ttcgacgcgt tgggcgggct ccggatcgac gtcccgtact
     1321 ccttcaaggg ttacgagaac accgatttca ccgagggccg caccacgatg acgggtgccg
     1381 agacgctcga gtactcgcgc acgcgcaagt cgctctccca cggggacttc gacagatcca
     1441 tgaaccaagg gcggatcctg ctcgcggcgt tctcgcagtt ccgagcggag ttccggaagg
     1501 atcctgccgc cctgttccag tggatcgcgg cggggatgcg caacgtctcc accgacctcc
     1561 cgctggacga gctgctcacg ctggcgttca cgtcgtggga gatccgtccc gcccgcctca
     1621 cgaacctggt ggcggtgggg ggcgtaggca cggcgggcgg ggccagcatc gtgagcctcc
     1681 cgtcgcccca cccggtgttc gaggacgtcg ccgccgacgg gttcatcctg cctcaggaca
     1741 tcccggccga cgccgaaccc gcgggctagc cccggcgttg tcgagccttc gcgtccgtcc
     1801 gtagcctgga gtgacattgg cgaaggagca gcccctgtct cggtctcctc gccggatccc
     1861 cctgatcggt gccggagtcg cggtgctgct cgtgatcggc gtccttgtct tcctcacgcg
     1921 tgaggacgcg ccgacggagg gggccggcgg cgaagagacc acgagcgacg gcgacgtcgc
     1981 gtcgccgacg acacccccgt tcgggttcga cgacacctcg cgcaagatgg tcaagacctc
     2041 gtcggagccg atggggtccc gccaccgaca agcgagcgtc gccgcggcga ccgaggccga
     2101 ggacgtgctc acggatctgt acacggatgg gttcctcgat ccggccaact gggagcgggg
     2161 gagctacgcg ggcgcgttcg gaggattcgc gcggggcgct cgcgaccagg cggagacgca
     2221 ccccggcccg ctcacggcag gacccggcgc cggggatcgc tacgagcgga tcgagcccaa
     2281 gtcgggccgg atcgccacgc ggatcctgct ggaccgccaa ggggccccgg tattgctcgt
     2341 gagcgtcgtt cggttctccg cggtcgcgac gggctcggac gaggtggtcc tccggtcggt
     2401 cgggcagtac ttcttcgagc gcgtgggccg cgcgtggagg atcgtgtcgt tcgacgtgac
     2461 ccggaacgac ggcccgagga aggccgcttg acccggcggg cggcggtcgc ggtggtggtg
     2521 ctcctcgcct gggtcgcggg gtccacggtg ggatcgatcg ccgagctccc ggccgcgtcc
     2581 gcccgcacgc ccggcatcgt gttcggccgg gcgcacggcg gctatacgcc ggatcccacc
     2641 ggtcggcgaa cgatcacgat cctcgcgatc ggatcggacg cccggccggg ccagaacccg
     2701 ctcgccctgc gcgcggactc gatccacgtg atcttcctgc atccgcggaa gcagcgagcg
     2761 gtcgtcgtcg gcatccctcg cgactcgttc gtgtcgatcc ccggcctggg cacgaccaag
     2821 atcaacgccg cgatgtcgag tggcggaccg gcgcgcatgg tgcagaccgt cgagcagaac
     2881 ttcggagcgc gcatcgacta ctgggccgtc acgtcgttct ggggcatcac gcgcatgttc
     2941 gagacgatcg gcgggctcca ggtacgggta ccgttccgga tgaaggatcg cttctccgag
     3001 tcgaacttcc accccggatt ccagcgcctc cgtgggcggc aggtcctggc g
//
LOCUS       DASUVE010000123         4385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_814300, whole genome shotgun sequence.
ACCESSION   DASUVE010000123 DASUVE010000000
VERSION     DASUVE010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814300"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VF036_02520"
     CDS             complement(<1..182)
                     /locus_tag="VF036_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264135.1"
                     /translation="MSVAGDPLPLLFDVHPGREIVRQGEPYPDAWVIRRGALLMETID
                     LEGHRLVLDVLGPGDLV"
     gene            284..706
                     /locus_tag="VF036_02525"
     CDS             284..706
                     /locus_tag="VF036_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6264136.1"
                     /translation="MAKAKLDTVIAAVPLFDTLSKRHVKKVAGLTTTVEYDAGDTVIQ
                     EGEPGDSFFVTVAGQAKVVSGGKMVHRLIPGDHFGEISLLDGRPRSASVVAETPLSLL
                     RLPRASFLRLVRDDADLARALLASLARMVRRVDRSLAK"
     gene            958..1125
                     /locus_tag="VF036_02530"
     CDS             958..1125
                     /locus_tag="VF036_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264137.1"
                     /translation="MLQPGDLEWFRAVAQFRVPAETGLVARTVPGVTRGGYDPAANYR
                     RFSEQIERLES"
     gene            complement(1143..>4385)
                     /locus_tag="VF036_02535"
     CDS             complement(1143..>4385)
                     /locus_tag="VF036_02535"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX6264138.1"
                     /translation="FPQEILDVLGGRPPTDEQWRAISWPLEPYVLVAGAGSGKTSVMA
                     ARVVYLALVALGRMPADHAGVLPGNVLCLTFTNKATENLQQRVRRALAGLDLPDGEEP
                     EITNYHGFAAQLLDRHGVLIGIEPNQRVLTPAQRVELCGRVLDRMTFEVVEAQRLPGV
                     VDRILTLDDQAANHFRSPDEIVEFNEHRLEELKEHRSDRAYGSARERIELAKACAVFR
                     ELKRELGVIDFGDQIELALDIVERHPEVAGQYRDRFAAVLLDEYQDTNVAQARLMAGV
                     FGGGHPVTAVGDPDQNIYAWRGASLFNLLEFPRQFARADGEEATKLPLYTNFRSGARI
                     LEAADVLIGQLPAAQRPDPDKRLVPWEENGPGEVHVARISDEVKEAAWVADRIVALHD
                     AGAAWSDCAVLCRTSRLFLSLQQAFVEREVPAEILGLAGLLKMPEVVEVLAYARATLD
                     PLASVALGRILLGPRYRVGFKDLALVASLAKTKSYALREEDEADGDAAPFLFTEALEH
                     LDEVEGLSEEGRARLEECRQELTELRAEARRPVGEFLGEIIRRTGILTELDAHLDVAT
                     ATAARRNLAAFLDEVHAFSPVQGELTLRAFLEYVDAVENLDKQEWSPVQPSEEDSVKV
                     MTIHVAKGLEFDHVFVPGLAKGLLPNTTIQHNPAERGKSMDFELRGDASYLPRYDGNL
                     SAFKKDLKQQEEYEERRTAYVAMTRARRSLHVTGAHWYAENMRAKEAGPFLRELKDWI
                     METKHATWDPGADIDEEHNPILGYRERFVRDWPGPALPAERDPVFPEGWRHAAVAVAE
                     SGGDIQDSLLEPLDQDGRAAFQRLGADRRGLALHLREREEVEGRGGEMTPPRNVSVGG
                     VIDYARCPKRFYWTSVRPLPRFSGPAARIGTEVHRWIERRSSGQGQLLELDDAPDLTA
                     DELATEPGKVDRLRQAFLASRFAGVVPLFAERAFLLRLDGFAVSGRIDAVFGAAVEGP
                     WEVVDWKTGRRPPADDPLAGLQLDLYGLACVEVWGKRPDDLTLTYLYLAGGEEVSHPM
                     GDPDEVRARVGDTLRAIGEGAFDPTPGPWCHHCDFLSFCDAGRRRVAADA"
BASE COUNT          661 a         1553 c         1502 g          669 t
ORIGIN      
        1 acgaggtctc ccggccccaa cacgtcgagg accagccggt ggccctcgag gtcgatcgtc
       61 tccatcaaga gcgcgccccg acggatcacc cacgcgtcgg ggtagggctc gccctggcgg
      121 acgatctccc gcccgggatg cacatcgaac agcaggggga gtgggtcgcc ggccacgctc
      181 acacgcacca cccttcgcgc gcgtgaccct atcgacggtc gccgacacgc cgtcggagag
      241 aacgcgctag gcttttgcca gcgcggtccc cgaggaggtc acgttggcga aggcgaagct
      301 cgataccgtc atcgcggcgg tgccgttgtt cgacacgctg tccaagcggc acgtgaagaa
      361 ggtcgcaggg ctcacgacga ccgtcgagta cgacgccggt gacacggtga tccaggaagg
      421 tgagccggga gactcgttct tcgtcaccgt cgcggggcag gcgaaggtgg tctcgggcgg
      481 caagatggtc catcgattga tcccgggcga ccacttcggt gagatctcct tgctcgacgg
      541 acgtccccgg tcggcgtcgg tggtcgccga gacgccgttg tccctgctcc gactcccgcg
      601 tgcgtcgttc ctgcgattgg ttcgggacga tgccgacctc gcccgggcgc tgctcgcctc
      661 gctcgccagg atggtcaggc gggtggaccg ctcgctcgcg aagtgagtcc gctgcgcatc
      721 gagttccacc acccgacgga accatcgacg ccggccgcgc ctgcgccggg tgcccctccc
      781 ccgccgcccc cacctcctcc tccgcccgcc gtcgccgccg cgacctggct cgaggggcgc
      841 ccggtcgtcg aggcgggcga tccggcgatc ggagcggcga tcgagcgtgc gttccgtccg
      901 acccccgtcg ttgtggacga tccatcgctc cgctccgcgg gcagccgtgg tgagcagatg
      961 ctccaacccg gggatctgga gtggttccga gcggtggcac agttccgggt cccagcagag
     1021 accgggctcg tcgctcgaac cgtcccgggc gtgacgaggg ggggatacga ccccgccgcg
     1081 aactaccgtc ggttctcgga gcagatcgaa cgcctcgagt cctgacctgt cggctccgaa
     1141 cctcaggcgt cggccgccac ccggcgacgt ccggcgtcac agaaggacag gaagtcgcag
     1201 tgatggcacc acggacccgg cgtgggatcg aacgctccct ccccgatcgc acggagcgtg
     1261 tccccgaccc gggctcgcac ctcgtcggga tcgcccatcg gatgcgagac ctcctcgccg
     1321 ccggcgagat acagataggt gagcgtgaga tcgtccgggc gcttgcccca cacttcgacg
     1381 caggccagcc cgtagaggtc gagctgcagg ccggcgagcg ggtcgtcggc cgggggacgc
     1441 cgaccggtct tccagtcgac gacctcccac ggaccctcga cggcggctcc gaacaccgcg
     1501 tcgatccgac ccgagacggc gaacccatcc agccgcagga ggaaggcgcg ctccgcgaac
     1561 agcgggacca cgcccgcgaa ccggctggcg aggaacgcct gccgcaagcg gtccaccttg
     1621 cccggctcgg tcgcgagctc gtccgcggtg aggtcgggcg cgtcgtccag ctcgaggagc
     1681 tggccttgtc cggaggaccg gcgctcgatc caccggtgga cctcggtccc gatccgggcg
     1741 gccggtcccg agaaacgagg gagcgggcgc acggacgtcc agtagaagcg cttcgggcag
     1801 cgggcgtagt cgatcacacc gcctaccgat acgttccggg gcggcgtcat ctcgccgcca
     1861 cgaccctcga cctcctcgcg ctccctcagg tggagcgcca ggccgcgacg gtccgcaccg
     1921 agccgctgga acgccgctcg gccatcctgg tcgagcggct ccaagaggga atcctggatg
     1981 tcgcccccgc tctcggcgac ggccacggcc gcatgccgcc agccctcggg gaacacgggg
     2041 tcgcgttctg cgggcagcgc gggccccggc cagtcgcgga cgaaccgctc ccggtagccc
     2101 aggatcgggt tgtgctcctc gtcgatgtcg gcgccggggt cccatgtggc gtgcttcgtc
     2161 tccatgatcc agtccttcag ctccctgagg aacggtccgg cctccttcgc gcgcatgttc
     2221 tccgcgtacc agtgggctcc ggtcacgtgc agcgaccgac gcgcacgggt catcgcgacg
     2281 taggcggtcc gccgctcctc gtattcctct tgctgcttca ggtccttctt gaaggcggag
     2341 aggttgccgt cgtagcgcgg gaggtaggag gcgtcaccgc ggagctcgaa gtccatcgac
     2401 ttgccgcgtt cggccggatt gtgctggatc gtggtgttcg gcaggagccc tttcgcgagc
     2461 ccgggcacga agacgtgatc gaactcgagg cccttggcaa catggatcgt catgaccttg
     2521 acggagtcct cctcggaggg ttggacgggg gaccattcct gcttgtccag gttctcgacg
     2581 gcgtcgacgt actcgaggaa cgcgcgaagg gtgagctcac cctgcacggg ggagaacgcg
     2641 tgcacctcgt cgaggaacgc cgccaggttg cgccgggccg ccgtcgccgt cgccacgtcg
     2701 aggtgggcgt cgagctcggt gaggatgccc gtccgtcgga tgatctcgcc gaggaactca
     2761 ccgacgggcc tccgcgcctc cgcgcggagc tccgtcagct cctgccggca ctcctccagt
     2821 cgcgcccgtc cttcctcgga gagaccctcg acctcgtcga gatgctcgag tgcctcggtg
     2881 aacaggaacg gagcggcgtc cccgtcagcc tcgtcctcct cccgcagcgc gtagctcttc
     2941 gtcttggcga gcgatgcgac gagtgccagg tccttgaagc cgacccgata gcgtggtccc
     3001 aggaggatcc ggccgagcgc gacgctcgcc agggggtcga gcgtggcgcg cgcataggcg
     3061 agcacctcga cgacctccgg catcttgagc aaccccgcga gtccgaggat ctccgcgggc
     3121 acctcgcgct cgacgaacgc ttgctgcagg gagaggaaca gcctcgaggt gcggcagagc
     3181 acggcgcagt ccgaccatgc agcgcccgcg tcgtgcagcg cgacgatccg atccgccacc
     3241 cacgccgcct ccttcacctc gtcggagatg cgcgcgacgt ggacctcgcc gggcccgttc
     3301 tcctcccacg ggacgagccg cttgtccggg tcggggcgtt gcgcggcggg gagctggccg
     3361 atcagcacgt cggccgcctc gaggatccgc gcacccgagc ggaagttggt gtacaggggg
     3421 agcttcgtgg cctcctcgcc gtccgcccgg gcgaactgcc tcgggaactc gagcaggttg
     3481 aacagcgacg caccacgcca ggcatagatg ttctgatcgg ggtcaccgac ggccgtcacc
     3541 gggtgaccac ctccgaagac gcctgccatc agccgcgcct gggccacgtt cgtgtcctga
     3601 tactcgtcga ggagcaccgc ggcgaaccga tcgcgatact ggccggcgac ctcgggatgc
     3661 cgctcgacga tgtcgagggc gagctcgatc tgatcaccga agtcgatcac accgagctct
     3721 cgcttcagct cccggaagac cgcgcacgcc ttcgcgagct cgatccgctc gcgagccgat
     3781 ccgtaggcgc ggtcggatcg atgctccttc agctcctcca accgatgctc gttgaactcg
     3841 acgatctcgt ccggcgagcg gaagtggttc gctgcctgat cgtcgagcgt gaggatccga
     3901 tcgacgacgc cggggagacg ctgcgcctcg acgacctcga acgtcatgcg gtcgagcacg
     3961 cggccgcaga gctcgacccg ctgcgcgggc gtgagcacgc gctggttcgg ctcgatcccg
     4021 atcaggacgc cgtgccgatc gaggagctgg gccgcgaagc cgtgatagtt ggtgatctcg
     4081 ggttcctcgc cgtcggggag gtcgaggccg gccagggcac ggcggacgcg ttgctggagg
     4141 ttctccgtcg ccttgttcgt gaacgtcaga cagaggacgt tcccgggcag gacgccggcg
     4201 tgatcggcgg gcatccgacc gagcgccacg agcgccaggt acaccacgcg cgccgccatc
     4261 accgacgtct tccccgaacc ggcaccggcc acgagcacgt acggctcgag cggccacgag
     4321 atcgcgcgcc actgctcatc ggtcggaggt cgtccgccga ggacgtcgag gatctcctgc
     4381 gggaa
//
LOCUS       DASUVE010000124         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_814302, whole genome shotgun sequence.
ACCESSION   DASUVE010000124 DASUVE010000000
VERSION     DASUVE010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814302"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VF036_02540"
     CDS             complement(<1..1001)
                     /locus_tag="VF036_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX6264139.1"
                     /translation="MSTLAVTTVDLARIQFATTSLYHFLFVPLTLGLAPLVAVMQTQW
                     YRTGNDAWLRLTRFFGTLMLINFAIGVATGLVQEFQFGMNWSIYSEFVGDVFGAPLAI
                     EGLAAFFLESTFLGLWIFGWHKLGPRLHLATVWLFALGSWLSALFIIVANSWMQRPVG
                     STLRDGRAELTSVWELLTNRFAIWAFVHVILVGLTTAAVVVLGVSCWHFVRGRNVEIF
                     RPAAKLALIVLVPVSAVNLLVGSYLGEVVTEYQPMKIAATEALWDTEQPAAFSVFQIG
                     GFSEDDQTPSFDIEIPKLLSLLATGSLDGEVQGMNELQAENEQEYGPGNYVPPVKAT"
     gene            complement(1162..1344)
                     /locus_tag="VF036_02545"
     CDS             complement(1162..1344)
                     /locus_tag="VF036_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264140.1"
                     /translation="MQRFIVTIEGPGWTDVDEVELPRPPNDGDTIETKFGICVVTNTD
                     LQSSAQHDGKIVCRLP"
     gene            1430..>2085
                     /locus_tag="VF036_02550"
     CDS             1430..>2085
                     /locus_tag="VF036_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264141.1"
                     /translation="MPEGLSATEVGKEIGEHARHAGGHGRHDRANAIGEALLLSIVTI
                     VAAWSGYSAAKWGTESSLALAEASTTRTKANREFQESLTFRVGDATTFNAWFDAYLAE
                     DQEAVEVAERRFRPEFRVAFDAWLATDPFENPDAPPGPQAMPEYVPTGLTDSRRLDDE
                     ADGLFAKGHEAAETADNYVRTTLILASVLFLVGISTQFPLRAVRYGLIAVGGALLVFA
                     G"
BASE COUNT          360 a          693 c          709 g          323 t
ORIGIN      
        1 tacgtggcct tgaccggcgg gacgtagttc cccgggccat actcctgctc gttctccgct
       61 tggagctcgt tcatgccctg cacttctccg tcgagggagc ccgtggcgag gagcgagagg
      121 agcttcggga tctcgatgtc gaagctgggc gtctggtcgt cctcgctgaa cccgccgatc
      181 tggaagacgg agaaggcggc cggctgctcc gtgtcccaaa gcgcctcggt cgccgcgatc
      241 ttcatcggct ggtactcggt gacgacctcg ccgaggtagc tcccgacgag gaggttcacg
      301 gcggagaccg gcacgaggac gatcagggcg agcttcgcgg ccggcctgaa gatctcgacg
      361 ttacggcctc ggacaaagtg ccagcaggaa accccgagga cgacgacggc tgcggtcgtg
      421 agcccaacga ggatcacgtg aacgaaggcc cagatcgcga accggttcgt gagcagctcc
      481 cagacgctcg tgagctcggc gcggccgtcg cgcagcgtcg agcccacggg gcgttgcatc
      541 cacgagttgg cgacgatgat gaagagagcc gagagccagc ttccgagcgc gaagagccag
      601 accgtcgcca ggtggaggcg cggccccagc ttgtgccagc cgaagatcca gaggccgagg
      661 aacgtggact cgaggaagaa cgcggcgagg ccttcgatgg cgagcggagc gccgaagaca
      721 tcgccgacga actcggagta gatcgaccag ttcatcccga actggaactc ctgcacaagc
      781 cccgtcgcga cgccgatcgc gaagttgatc agcatcagcg tcccgaagaa gcgggtgagc
      841 cggagccagg cgtcgttccc cgtgcggtac cactgcgtct gcatgaccgc gacgagcggc
      901 gcgagcccca acgtcagcgg gacgaagagg aagtggtaga gcgacgtcgt cgcgaactgg
      961 atgcgcgcga ggtcgacggt ggtgaccgcg agcgtgctca cgctgccgtt aggtcatggt
     1021 cgaaggcgtg gcttgtcacc ctgccctttc ctggcgtcag aactccggca ggatcgtgcc
     1081 agatttcgcc acgaccatgc cgcgacctcg gtcgctggac cgtgagtcgg tccttgcctc
     1141 tcggaaccgc ggatgggcgg gctacgggag acggcaaacg atcttgccgt cgtgttgcgc
     1201 cgagctctgg aggtccgtat tcgtgacgac gcaaatcccg aacttggtct cgatcgtgtc
     1261 gccgtcgttg gggggcctcg ggagctccac ctcgtcgaca tccgtccacc ccgggccctc
     1321 gatggtgacg atgaagcgct gcaccgatgg cccatgatgt cagactcagg gatctgaatc
     1381 acacggaccc caggagcggt cgtcaccccc cgtcggccac aatcgctggg tgcccgaggg
     1441 gctctcggcg accgaggtcg gcaaggagat aggcgagcac gcccggcatg cgggcgggca
     1501 cggccggcac gaccgcgcga atgcgatcgg cgaggcgctc ctcctctcca tcgtcacgat
     1561 cgtcgccgcg tggtcgggct actcggcggc aaagtgggga acggagtcgt cgcttgcgct
     1621 cgccgaggcc tccacgaccc gaacgaaggc aaaccgtgag ttccaggagt cgctgacctt
     1681 ccgcgtcggc gacgcgacca ccttcaacgc ctggttcgac gcctacctcg cggaggacca
     1741 ggaggcggtc gaggtcgccg agcgccggtt ccggcccgag ttcagggtcg ccttcgacgc
     1801 gtggctcgcc accgacccct tcgagaatcc cgatgcgccg ccggggccgc aggcaatgcc
     1861 cgagtacgtt ccgaccggcc tgaccgactc gcgccggctc gacgacgagg ccgacggcct
     1921 cttcgcgaag gggcacgagg ccgccgaaac cgccgacaac tacgtccgca ccacgctcat
     1981 cctggcgagc gtcctcttcc tcgtcgggat cagcacccag ttcccgctgc gcgccgttcg
     2041 ctacggactg atcgccgttg gcggggcctt gctcgtcttc gccgg
//
LOCUS       DASUVE010000125         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1003773, whole genome shotgun sequence.
ACCESSION   DASUVE010000125 DASUVE010000000
VERSION     DASUVE010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003773"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VF036_02555"
     CDS             <1..382
                     /locus_tag="VF036_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6264142.1"
                     /translation="VIAGILIAVAAHLWREIRLNVHTRVEDATLHVHPRGVLYYASAP
                     SLEDSVGRLLHEHQAATRLVVHLDGLGRIDLTGALALRNLLIDAREAGLDVQVVDVPL
                     QAAKTVERTLVEVVPVTMLDEPED"
     gene            1093..1494
                     /locus_tag="VF036_02560"
     CDS             1093..1494
                     /locus_tag="VF036_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264143.1"
                     /translation="MPACEEVGAGSGATSGSPTEGSIVDGGSPPTTLFVTESTRSRAV
                     GHMATPARPIARSAPLDKPTFIARRRFGRREVARIAAHAIRPPTVTSRANVSASARMA
                     RSSMDMRSWSVGGTGGSEGGPTPDRLVLTPE"
     gene            1819..>2625
                     /locus_tag="VF036_02565"
     CDS             1819..>2625
                     /locus_tag="VF036_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX6264144.1"
                     /translation="MYGNLANADLDDDGLVSVADALNRHIAAAQVALLGVIAEIDRRG
                     AWRDSGARDLAHWLSIRYGVSWWKADRWIHAANALHGLPGLAAALEAGTIGIHKVVEL
                     SRFATPDTEDELVEWACESTCATIRRRADLDVKAAHEETMEFERNRSLRYWYEDGGRR
                     FGLEAHMPSAQGAIVAKALQRVVDQVPVMPAEHDAAYIDARRADALLALCSQHIAADA
                     DADRATVVVHASLEALAGRRNAETDHGAVVPPEALQRMPCDARVQVVAENQ"
BASE COUNT          392 a          880 c          987 g          366 t
ORIGIN      
        1 cgtgatcgcc ggcatcctga tcgccgtggc cgcacacctc tggcgcgaga tccggctgaa
       61 cgtgcacaca cgcgtggagg acgcgacgct ccacgtccac ccgcggggcg tcctctacta
      121 cgcatcggcg ccgagcctgg aggacagcgt ggggcggctg ctccacgagc accaggccgc
      181 gacccgcctg gtggtgcatc tggacgggct cggccggatc gacctcaccg gcgccctcgc
      241 gctccgcaac ctgttgatcg acgcgcggga ggcgggcctc gatgtccagg tcgtcgacgt
      301 gccgctgcag gccgcgaaga cggtcgagcg cacgctcgtc gaggtggtgc cggtgacgat
      361 gctcgacgag cccgaggact gacgcgcatg accaccgacg gccccttacc gcggtgtgct
      421 gtagttcgga tggggggtcg gccggactcc ccgtcgcccg cgtcccgatg aggcgctagc
      481 ctgtgaggcg acgcgcacct cccgatcgtg gcgacgagcc gcagcgccgc acgcccggcg
      541 gtccgcggtc cgggggggat cgcgtcgacg tggaggcccc cggccttccg gctaccgtgc
      601 cggcgccccg ccacggcagc acccccccgg gggtcaccgc gctactgctt gcttctcgcg
      661 gtggcacgcc ggtgtcgcgc cgcgccggag acgtggacgg agcagggcta ccggaccgag
      721 agcctgttcg tgaggaagcc gtggagtttc tccctcggcc gatcgccccc gtatgaccct
      781 cagacgggct ggggcaggat cggagggtcg atcggcggac cgacgggcgg gtcgatcagg
      841 gcggagtccg agggagacgg ggagggagat ggcgtcgggg agggggatgg gggcggcgac
      901 ggcgagggat gaggagaagg ggacggcgac ggagacggtg ggggagacgg cgacgaagtg
      961 gggctggggt ccggactggg gtccgtcccc gggcccggat cggcgctcgg gcctcctccg
     1021 gaaccgtcgt tgcccgaggt cgacgggtcg acggccccgt cgctcccggt cggtcccgcg
     1081 acacggctcg gcgtgcccgc ctgtgaggaa gtcggggcag gttcaggagc cacgagcggc
     1141 tctcccacgg aggggtcgat cgtcgacggc ggttcaccgc cgaccacgct gttcgtgacg
     1201 gagtccacgc gctcacgagc cgtgggccac atggcgaccc cggcgaggcc gatcgccagg
     1261 agcgccccgc tggacaagcc caccttcatc gcacgacgca gattcgggcg gcgcgaggtc
     1321 gcccggatcg cggcccacgc gatcaggccg ccgacggtca cgagcagggc gaacgtcagc
     1381 gcgtcggcga ggatggcgag gtcgtcgatg gacatgagat cgtggtcggt gggcggaacg
     1441 ggtggatcgg aaggtggccc aacgcccgac cggctcgtgc tcaccccaga gtaaccgctc
     1501 gacgggctac tcgaaacact cgcggatgac ggatggaccg cctccctccg gggcaccccg
     1561 gtgcgacaag tcacccggga tcggcccaac cgatggggga ctcacgcctc tggcgccgag
     1621 atcccttccc gcgcaagatg tgagcggaag gtctgggtcg gggcgatggt ggtgatcgcg
     1681 gcgctcgccg cgggacggtc ttcgcgtggc agcgcggcca acatgagggc agtccatccc
     1741 ctagcgatcg cgagaaccgg cgtccggatg tgacgtccgt cacggtgttc ggtaggcgcg
     1801 ggtcctaggc tcgaacctat gtacgggaac ctcgcgaacg ccgatctcga cgacgacggt
     1861 ctggtcagcg tcgccgatgc gctgaaccga cacatcgcgg ctgcgcaggt ggcactgctc
     1921 ggcgtgatcg ccgagatcga tcgtcgtggg gcatggcgcg actcgggagc acgggacctc
     1981 gcgcattggc tctccatccg ctacggcgtc tcgtggtgga aggccgaccg ttggatccat
     2041 gccgcgaacg cgctccatgg tttgcccggc ctcgcggccg ccctcgaggc cggcacgatc
     2101 ggcatccaca aggtggtcga gctgagtcgg ttcgccacgc ccgataccga ggacgagctc
     2161 gtggaatggg cgtgcgaatc cacgtgcgcc acgatccgtc gtcgcgccga cctggatgtc
     2221 aaggcggctc acgaggagac gatggagttc gagcgaaacc gctcgctgcg ctattggtac
     2281 gaggacgggg ggcgcaggtt cggtctcgag gcgcacatgc cgtccgccca gggagcgatc
     2341 gtcgcgaaag cgctgcagcg ggtggtcgat caggtccccg tcatgccggc ggagcacgat
     2401 gccgcgtaca tcgacgcccg ccgcgccgac gctctgctgg cattgtgttc gcagcacatc
     2461 gccgccgacg ctgatgccga ccgggcgacc gtcgtggtcc atgcatcgct cgaggcgctc
     2521 gcggggcgcc gcaacgccga gactgaccac ggcgcggtcg tgccgccgga ggcgctacag
     2581 cgcatgccct gcgatgcccg tgtccaggtg gtcgccgaga accag
//
LOCUS       DASUVE010000126         8417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1193152, whole genome shotgun sequence.
ACCESSION   DASUVE010000126 DASUVE010000000
VERSION     DASUVE010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8417
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193152"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VF036_02570"
     CDS             complement(<1..565)
                     /locus_tag="VF036_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX6264145.1"
                     /translation="MTDVMQDTLIPLAYLFAAACFVLALKWLSHPTTARRGVRTGEIG
                     MLVAVVATLVDLRIVEYRWIGVAIVAGVALGVPLGLLVPMTAVPQRTALSHAFGALAS
                     GLVGTAEYYLRRPEIDGTTMVAIGLEVVLGFLTVTGSLIAAGKLQGTLPDRPWVYPGQ
                     NIVNVSVFSVALILVGMLVIDPGREMLF"
     gene            complement(562..879)
                     /locus_tag="VF036_02575"
     CDS             complement(562..879)
                     /locus_tag="VF036_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX6264146.1"
                     /translation="MSQALEVGLFIFVIASFLGYTVISRVPGLLHTPLMSLTNALSAI
                     SIVGSLILAGARETGFATVLASIAVFAATTNIVSGFLITDRMLKMFRRREAPEEPKEG
                     MSS"
     gene            complement(863..2038)
                     /locus_tag="VF036_02580"
     CDS             complement(863..2038)
                     /locus_tag="VF036_02580"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX6264147.1"
                     /translation="MERLVVGVPTETFPGEARVALVPSVVRRLSDAGMDVCVEHDAGE
                     RAGFPDESYAAHGARIGTRADVFRASIIAQVRTSGMNPENGRDDIDRLHPGHVVVGMS
                     DALGSPEGVRQAAGRGIISFALDLMPRITRAQSMDVLSSQATVIGYKAVLLAANDLPK
                     MFPMLTTAAGTVPPAQVLVIGAGVAGLTAIATARRMGAVVQGYDVRPSAQEDIESLGA
                     RCVLLPLQPGDAEDPAGYAKELGAAFYRRQQELIAQVVAGVDVVITTALIPGKRAPVL
                     VTEEAVLGMPAGSVIVDCAAERGGNCELTDPDNDVVTENSVTILGPTNLASTVPHEAS
                     LMYAKNMVAFLLHVVREGELAIDLEDEIIAGTLVTREGEIVNPRVLGSLEVSSVPGA"
     gene            complement(2041..3078)
                     /locus_tag="VF036_02585"
     CDS             complement(2041..3078)
                     /locus_tag="VF036_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012629726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HEX6264148.1"
                     /translation="MSVDLRTTYLGLELVNPLVPSASTLSSRIDNLKRLQGAGASAIV
                     MQSLFEEQIEHEELQTHRVLENGAESFPEALSYIPEMEEYNTGPDEYLRHLAACKREL
                     EIPVIGSLNGASEGGWIRYAKLIQDAGADALELNVYFIAADPDESGSQVERRYLDLVS
                     AVRGSVSIPLAVKVGPYFSSMANMARQLEAAGANGLVLFNRFLQPDIDLDTLQVDPTL
                     HLSTSDELRLPLRWIAMLHGKVACSLAATSGVWTADDAVKLLLAGADVTMMASALFEH
                     GPEYLGVVLEGVRTWLDEHEYESVEQLKGSVSQANVADPLAFARSNYMQMLVNFTSPY
                     DWREIPGSVQS"
     gene            complement(3075..6701)
                     /gene="nifJ"
                     /locus_tag="VF036_02590"
     CDS             complement(3075..6701)
                     /gene="nifJ"
                     /locus_tag="VF036_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEX6264149.1"
                     /translation="MTEARSMIAVDGNEAAARIAHRLSEVIAIYPITPSTSMGELADA
                     WSAAGRTNLWGAVPQVTEMQSEGGAAGALHGALQGGALCTTFTASQGLLLMIPNMYKI
                     AGELTPAVIHVAARTLATHALSIFGDHSDVMAVRQTGWAMLASSDVQAAQDLAAIAHA
                     ATLECRVPFLHFFDGFRTSHEVNVITELDDDALRVLVDGHMVRAHRERALSPDHPVLR
                     GTAQNPDVFFQAREASNPYYAIIPHVVQQTMDRFAELTGRAYHLFDYHGAADAERVVV
                     LMGSGEGAAREAVDELIARGEKVGILNVRLFRPFSAEAFVGALPSTVRAIAVLDRTKE
                     PGAAGEPLYQDVVTVLAEQVAAGAVPTMPTVIGGRYGLASKEFDPAMAKAVFDELAMD
                     RPKDHFTVGIVDDVSHTSLEVDRSFTTEADDVVRAVFYGLGADGTVSANKSSVKIIGE
                     GTDLFAQGYFVYDSKKSGSVTTSHLRFGPRPIHSTYLIGSGQASFVACHQFGFLERLD
                     VLDVAAPGATLLLNSPFDPDAVWEHLPVETQQVIIEKELRLYVVDAQKVATAAGLGLR
                     VNTILQTCFFALANVLDQAEAVARIKAAIEKSYGKRGRAVVEQNFTAVDAALDSLHEV
                     PVPAGIDGDLHRSLPVPEDAPDFVQRVTALMIAGKGDLLPVSAMPVDGTFPTDTARWE
                     KRSIAQEIPIWDPEICIDCGKCALVCPHAAIRMKVYEPITADSAPETFQHKEWRDRDN
                     PGMWMTIQVTPDDCTGCGVCVDVCPAKSKEVVKHKAIDMAPKSEHVDAERANLDFFLD
                     IPPADRREVKVASIKGSQMLEPLFEFSGACAGCGETPYLKLLSQMFGDRILVANATGC
                     SSIYGGNLPTTPWSRDANGRGPAWSNSLFEDNAEFGLGMRLALDHQAAYARALLGDLG
                     GSIDEDLARAILDAPQDDEAHLADQRERVAQLKKRLEAREDDEARNLLAIADALVRKG
                     VWIVGGDGWAYDIGFGGLDHVLASGRDVNVLVLDTQVYSNTGGQASKATPRGAVAKFA
                     AGGKPIGRKDLGLFATGYGNVYVAQVALGGDNAQTVKAFAEAESWPGPALIIAYSTCI
                     AHGIDMSTSMSHQAEAVKSGFWPIWRYDPRLAVPGQRPMQLDSRAPSLPLKEFQMKEA
                     RFAMLARSDPEGYAELQVLAQEDAEERRRLYEQLAGVERHAPGRADDGGDERAPADTE
                     GEGR"
     gene            complement(7105..7488)
                     /locus_tag="VF036_02595"
     CDS             complement(7105..7488)
                     /locus_tag="VF036_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6264150.1"
                     /translation="MGRPIMHFEIASPNLERAAAFYEELFDWDVGEEQMDGYRLVGTT
                     DGSIGGGLLRTPEGVFPYVTIYVGVENLVETLERAEELGGKTIVDPMPIPGAGRFAMF
                     QDPDGVMLGLFEEKPESGSGTPEAR"
     gene            complement(7613..7813)
                     /locus_tag="VF036_02600"
     CDS             complement(7613..7813)
                     /locus_tag="VF036_02600"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HEX6264151.1"
                     /translation="MAIQGGGKLVAVGIADAGWDSVSTRAIAQYRPDGTLDPTFSGNG
                     KLWGANAGRAMTVPLLGPFTLA"
     gene            complement(7887..>8417)
                     /locus_tag="VF036_02605"
     CDS             complement(7887..>8417)
                     /locus_tag="VF036_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6264152.1"
                     /translation="LGRVSMHVLHHAPCSVVVTHRAPTGSVRVLVGADGSEGAGSSLG
                     SAAEVLDRDRCTIEVATVVSHPWMFAVTYPAGTFLGHAPDPQGFEKEKIEQGRLIARR
                     ASAEASAAGFKVMEEAVLVGRPGHQLLEEADDIGADLVVVGSRGHGAMSRTLLGSVSD
                     QVARHAPATFVGRLVG"
BASE COUNT         1354 a         2917 c         2768 g         1378 t
ORIGIN      
        1 ggaagagcat ctccctgccg gggtcgatca ccagcatccc gacgaggatg agagccacgc
       61 tgaagacgga cacgttgacg atgttctggc cggggtacac ccagggccgg tcgggaaggg
      121 tcccctggag cttgccggcc gcgatcaacg acccggtcac cgtcaagaag cccaggacga
      181 cctcgagccc gatggcgacc atcgtcgttc cgtcgatctc cggccgccgg aggtagtact
      241 cggccgtccc gacgaggccc gacgccagcg ccccgaaggc atgggataac gcggtcctct
      301 ggggaaccgc ggtcatcggg accagcaggc cgagcgggac ccccagcgcc actcccgcca
      361 cgatcgccac accgatccat cggtactcga cgatccggag atcgacgagc gtcgccacga
      421 cggcgacgag catgccgatc tcgccggttc gcacgccgcg cctcgcggtc gtgggatgcg
      481 agagccactt caacgcgagg acgaagcagg ccgccgcgaa caggtaggcg agtgggatga
      541 gggtgtcctg catgacgtcg gtcacgacga catcccctcc ttcggctctt cgggtgcctc
      601 acgcctcctg aacatcttca acatgcgatc cgtgatcagg aatccagaaa cgatgttggt
      661 cgtggcggcg aagaccgcga tcgaggcgag cacggtcgcg aaccccgtct ctcgtgcccc
      721 cgcgagtatc agggaaccga cgatcgagat cgcggacagc gcgttggtga gggacatcag
      781 gggggtgtgc agcaagccgg ggaccctcga gatgaccgta tacccgagga acgaggcgat
      841 cacgaagatg aagaggccga cctcaagcgc ctgggacact gctcacctcg agagatccga
      901 gcacgcgagg gttgacgatc tcgccttccc gggtcaccag cgtgcccgcg atgatctcgt
      961 cctcgagatc gatcgccagc tccccctcgc gcacgacgtg cagcaggaag gcgaccatgt
     1021 tcttcgcgta catgaggctg gcctcgtgcg gcaccgtgga cgcgaggttc gtcggaccca
     1081 agatggtcac cgagttctcc gtgacgacgt cgttgtccgg gtcggtgagc tcgcagttcc
     1141 cgccgcgctc cgcggcgcag tccacgatca cggaacctgc gggcatcccg aggaccgcct
     1201 cctccgtgac caggacgggt gccctcttcc cggggatcag cgcggtggtg atcaccacat
     1261 cgacgccggc gacgacctga gcgatcaact cctgctgccg ccggtagaag gcggcgccga
     1321 gctccttcgc gtagcccgcg ggatcttcgg cgtcacccgg ctgcaggggg aggagcacgc
     1381 accgcgcgcc gaggctctcg atgtcctcct gcgcgctcgg gcgcacatcg tagccctgga
     1441 ccacggcccc catccggcgg gcggtcgcga tcgccgtcag gccggcgacg cccgcgccga
     1501 tgaccagcac ctgcgccggc gggacggtcc ccgcggcagt ggtgagcatc gggaacatct
     1561 tcggcaagtc gttcgccgcc aggaggacgg ccttgtagcc gatcacggtt gcttgcgacg
     1621 agagcacatc catgctctgc gcgcgcgtga tccgcggcat gaggtccagc gcgaagctga
     1681 tgatcccgcg gccggcggcc tgccggacgc cttcggggga accgagtgcg tccgacatcc
     1741 cgaccaccac gtgaccgggg tggagccgat cgatgtcgtc gcggccgttc tccgggttca
     1801 tgccggaggt tcgcacctgc gcgatgatgg acgcacggaa cacgtccgcg cgcgtcccga
     1861 tgcgcgcacc atgtgccgcg taggactcat cggggaagcc tgcgcgttct cccgcgtcgt
     1921 gctccacgca gacatccatg ccggcgtcgg acagtcggcg caccacggag ggcacgagcg
     1981 ccacgcgagc ttcccccggg aacgtctcgg tgggcactcc gacgacgagc ctttccatgc
     2041 tcaggactgc acgctcccgg ggatctccct ccagtcgtac gggctcgtga agttgacgag
     2101 catctgcatg tagttcgagc gcgcgaacgc cagagggtcg gccacgttcg cttggctcac
     2161 gcttcccttc agctgctcga cgctctcgta ctcatgctcg tccagccacg tccgcacgcc
     2221 ctcgagcacc actcccaggt actcgggacc gtgttcgaag agagcgctcg ccatcatcgt
     2281 cacatcagcc ccggcgagca ggagcttcac cgcgtcgtcg gccgtccaca cgccactcgt
     2341 cgcggccaga gaacatgcga ccttcccgtg gagcatcgcg atccaccgca gggggagtcg
     2401 cagctcatcg ctcgtcgaga ggtggagggt cgggtcgacc tgcagcgtgt cgaggtcgat
     2461 gtccggctgg aggaagcggt tgaagagcac gagcccgttc gccccggctg cttcgagctg
     2521 ccgagccatg ttcgccatgg atgagaagta ggggccaacc ttcacggcca gagggatcga
     2581 gacgcttccg cggacggcgg agacgaggtc gaggtatcgc cgctcgacct ggctgccgct
     2641 ctcgtcggga tcggccgcga tgaagtacac gttcagctcg agcgcatcgg cgcccgcgtc
     2701 ttggatcagc ttcgcgtagc ggatccatcc accctcgctc gctccgttca gggagccgat
     2761 caccgggatc tcgagctcgc gtttgcacgc cgcgaggtgt cgcaggtact cgtcgggacc
     2821 ggtgttgtac tcctccatct ccgggatgta ggagagcgcc tccgggaagg actccgcgcc
     2881 gttctcgagg acgcggtgcg tctggagttc ctcgtgctcg atctgctcct cgaacaacga
     2941 ctgcatcacg atcgccgacg cacccgcgcc ctggagtcgt ttgaggttgt cgatgcgcga
     3001 cgacaaagtg gatgccgacg gaacgagcgg gttcaccagc tcgagtccga gatacgtggt
     3061 ccggagatcg acgctcatct gccttcccct tccgtgtctg cgggcgcccg ttcgtccccg
     3121 ccatcgtctg cgcgacccgg cgcgtggcgc tcgaccccgg cgagctgctc atacagccgc
     3181 cgacgctcct cggcgtcttc ctgcgcgagc acctggagct ccgcgtagcc ttccggatcg
     3241 gagcgggcaa gcatcgcgaa ccgagcctcc ttcatctgga actccttcag cggtagcgac
     3301 ggagccctgc tatcgagctg catgggtcgt tgccccggga cggcgagacg tgggtcgtac
     3361 cgccagatcg gccagaagcc gctcttgacc gcctcggcct ggtgcgacat cgaggtcgac
     3421 atgtcgatcc cgtgggcgat gcacgtcgag tacgcgatga tcagggccgg ccccggccac
     3481 gactcagcct cggcgaacgc cttcaccgtc tgcgcgttat ccccgccgag cgcgacctgc
     3541 gccacgtaga cgttcccgta gcccgtcgcg aacagcccga gatccttgcg gccgatcggc
     3601 ttgcctcccg ccgcgaactt cgcaacggcc ccgcgcggcg tcgccttgga ggcctgcccg
     3661 ccggtgttcg agtagacctg cgtgtcgagc acgagcacgt tgacgtccct gccgctcgcc
     3721 agcacgtgat cgagcccccc gaaaccgatg tcgtaggccc acccgtcgcc gccgacgatc
     3781 cagacgcctt tgcggacgag cgcatcggcg atcgcgagca ggttccgagc ctcgtcgtcc
     3841 tcgcgggcct cgagccgctt cttcagctgc gcgacgcgct cgcgctgatc ggcgaggtgt
     3901 gcctcgtcgt cctggggtgc atcgaggatc gcgcgggcga gatcttcgtc gatcgacccg
     3961 ccgagatcgc cgagcagggc gcgggcatac gccgcctggt ggtccagagc gagccgcatc
     4021 ccgagtccga actccgcgtt gtcctcgaac agggagttcg accacgcggg ccctcggccg
     4081 ttcgcgtccc gcgaccaagg cgtcgtcggg aggttgccgc cgtagatcga ggagcagccg
     4141 gtggcattcg cgacgaggat ccggtcgccg aacatctgtg acaggagctt caggtacggg
     4201 gtctcgccgc acccggcgca ggcaccgctg aactcgaaga gtggctcgag catctgcgag
     4261 cccttgatcg aggcgacctt cacctcgcgt cggtcggccg gagggatgtc gaggaagaag
     4321 tcgaggttgg cccgctccgc gtccacgtgc tcggacttcg gcgccatatc gatcgccttg
     4381 tgcttcacga cctccttcga cttggcgggg cagacgtcga cgcacacccc acatccggtg
     4441 cagtcatcgg gagtgacctg gatggtcatc cacatccccg ggttgtcccg gtcacgccac
     4501 tccttgtgtt ggaacgtctc cggcgccgag tcggcggtga tcggctcgta caccttcatc
     4561 cggatcgccg cgtgcggaca cacgagcgcg cacttcccgc aatcgatgca gatctccggg
     4621 tcccagatcg ggatctcctg cgcgatcgag cgcttctccc atctcgcggt gtcggtcggg
     4681 aacgtgccat cgaccggcat cgcggaaacc gggagcaggt cgcctttccc cgcgatcatc
     4741 aaggcggtca cgcgctggac gaagtcgggg gcgtcctctg ggaccggcag cgatcggtgc
     4801 aggtcaccgt cgatgcccgc cggcaccgga acctcgtgga ggctgtcgag cgcggcgtcc
     4861 accgcagtga agttctgctc cacgacggcg cggccgcgtt tgccatagct cttctcgatc
     4921 gcggccttga tgcgagcgac ggcctcggcc tgatcgagga cgttcgcgag ggcgaagaag
     4981 caggtttgca ggatcgtgtt cacgcgcagg ccgagtcccg cggcggtcgc gaccttctgg
     5041 gcgtcgacga cgtagaggcg gagctccttc tcgatgatca cctgctgggt ctcgaccggg
     5101 aggtgctccc agaccgcatc cgggtcgaac gggctattca agagcaacgt cgctcccggc
     5161 gccgccacat ccaggacgtc gaggcgctcg aggaagccga actgatggca ggcgacgaag
     5221 ctcgcctgac ccgacccgat caggtaggtc gagtggatcg ggcgcggtcc gaaccgcagg
     5281 tgtgaggtcg tcacggatcc cgacttcttc gagtcgtaga cgaagtagcc ctgggcgaac
     5341 aggtccgtcc cttcgccgat gatcttgacc gagctcttgt tggcgctcac cgttccgtcg
     5401 gcgccgaggc cgtagaagac ggctcgcacg acatcgtcgg cctccgtcgt gaaggaacgg
     5461 tcgacctcca gcgaggtgtg cgagacgtcg tcgacgatgc cgacggtgaa atgatccttg
     5521 ggccgatcca tggcgagctc gtcgaagacg gctttggcca tcgccgggtc gaactccttc
     5581 gatgcgaggc cgtaccgtcc accgatgacc gtcggcatcg tcgggacggc acctgcggcg
     5641 acctgctcgg cgagcaccgt cacgacgtcc tgatagaggg gctcgcccgc tgcgcccggc
     5701 tccttcgtcc tgtcgagcac ggcgatcgcc cgaacggtag acggcaacgc accgacgaat
     5761 gcctcggccg agaacggtcg gaagagccgg acgttcagga tgccgacctt ctctccgcgt
     5821 gcgatgagtt cgtcgacggc ctcgcgcgcg gcgccctcac ccgagcccat gagcaccacg
     5881 acgcgttccg cgtcggcggc tccgtgatag tcgaagagat ggtaggcgcg accggtgagc
     5941 tccgcgaatc ggtccatcgt ctgctgcacc acgtgcggga tgatcgcgta gtaggggttc
     6001 gaggcctcac gagcctggaa gaacacgtcc gggttctggg cggtgccccg taggaccgga
     6061 tggtcggggg agagggcgcg ctcgcgatgc gcccggacca tgtggccgtc gacgagcact
     6121 cgaagggcgt cgtcatcgag ctcggtgatc acgttcacct catgcgacgt ccggaagccg
     6181 tcgaagaagt ggaggaacgg cacgcgacac tcgagtgtcg ccgcgtgggc gatcgcggcg
     6241 agatcctgcg cggcctggac atcggacgag gcgagcatcg cccagccggt ctgccgcacg
     6301 gccatcacat cgctgtgatc gccgaagatc gaaagcgcat gggtcgccaa cgtccgggcg
     6361 gcgacgtgga tcaccgctgg ggtgagctcg ccggcgatct tgtacatgtt ggggatcatc
     6421 aacaggagac cctgcgaagc cgtgaacgtt gtgcataagg cgccgccctg cagcgcgccg
     6481 tgcaacgcac ccgcggcgcc accctcgctc tgcatctcgg tgacctgcgg gaccgcgccc
     6541 cacaggttcg tgcggccagc cgccgaccag gcatcggcga gctcgcccat cgacgtcgat
     6601 ggcgtgatcg ggtagatcgc gatcacttcc gacaaccggt gcgcgatcct cgccgctgcc
     6661 tcgttcccgt cgaccgcgat catgcttcgg gcttccgtca tccggacctc ctcgcagtct
     6721 gctcgcgacc aggcaggcgc cgctccctac acgcggtccg tcctggcggg gtcattcgac
     6781 cctctcgtgc gatcaggggt ttgccccgcc gacgggcggc ggtgtggcga cgagacatcg
     6841 actcgggatc ggcgagcagt gcgctcagca tgtggaccac tcgatccagc ccatcagacg
     6901 ggccatggta agccctcgca cgggatcccg gagacgccgg aggacgcctt cccggccacc
     6961 gcggcgtcgg cccgcgtcca cgcgatgcga ggcccgggac cgcggccggc ggccgcggtc
     7021 atcgatcagg acgatccgac cgagccagcc gaccagggag tggccgcacc taccgtcgtt
     7081 cgacccgcct ctcaccggac cgcgctatcg cgcttcaggt gttccggatc cagactcggg
     7141 cttctcctcg aacaatccaa gcatcacgcc gtccgggtcc tggaacatcg cgaatctgcc
     7201 tgctccgggg atcggcatcg gatcgacgat cgtcttgccc ccgagctctt cggctcgctc
     7261 cagcgtctcc acgaggttct cgaccccgac gtagatcgtc acatacggga agacaccttc
     7321 gggtgtgcga agcaagccac ctccgatgct gccgtcggtc gtcccgacga ggcggtaccc
     7381 gtccatctgc tcttccccga cgtcccagtc gaacagctct tcatagaagg ccgccgctct
     7441 ctccaggttc ggtgacgcga tctcgaagtg catgatcggc cgtcccatcg tgctcacccc
     7501 tctcgcgggt tctctctgtc ttcgaccatg ctggatcgcc ctgacggtcg tcggacgggt
     7561 cgaacggcct gtcttcccgg tgaccttcgg ctgacatctc gcgcgccagg tatcaagcca
     7621 aggtgaacgg acccagcagg ggcaccgtca tcgccctccc cgcgttggcg ccccacagct
     7681 tcccgttgcc gctgaacgtg ggatccagcg tgccatcggg tcggtactga gcgatcgccc
     7741 gcgtcgagac gctgtcccag ccggcgtctg cgataccgac cgcgaccagc ttcccaccgc
     7801 cttggatcgc cacgtcgagc gcgcgatctc agcgaacgga aggaccggtt tcggttgctc
     7861 gccgccgtcg cgggtgacgg agtcgactag ccgacgagtc gacccacgaa cgtcgccggg
     7921 gcatggcgag cgacctggtc gctcacggat ccgaggaggg tgcggctcat cgccccgtgc
     7981 cctcttgagc ccaccaccac caggtcggcg ccgatgtcat cggcttcctc caggagctga
     8041 tgcccgggcc tgccgaccag gaccgcttcc tccatcacct tgaagccggc ggcgctcgcc
     8101 tcggccgagg cgcgtcgagc gatgaggcgg ccctgctcga tcttctcctt ctcgaagccc
     8161 tgggggtcgg gggcgtggcc gaggaacgtg ccggcgggat aggtgacggc gaacatccag
     8221 ggatggctca ccaccgtcgc cacctcgatc gtgcaccgat cgcggtccag gacctccgcg
     8281 gcggatccga ggctcgagcc ggctccctcg gagccgtcgg cgcccacgag gacccgtacg
     8341 gaaccggtcg gcgcacggtg cgtcacgacg accgagcacg gcgcgtgatg caggacgtgc
     8401 atgctgaccc ggccgag
//
LOCUS       DASUVE010000127         2425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_251979, whole genome shotgun sequence.
ACCESSION   DASUVE010000127 DASUVE010000000
VERSION     DASUVE010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251979"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VF036_02610"
     CDS             complement(<1..449)
                     /locus_tag="VF036_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6264153.1"
                     /translation="MATDKAVTGDVDWASIATTGGRRTAHEFVKDSLRRAILRGDLSG
                     GSRLIQADLATMLNVSTTPVREALRDLATEGFITLDRHRGGVVRELNWSEMEEIRLIR
                     HQLEPLAVRLVVEKITEEELQEAERLRERMAKERDLGNWVELNTQFHL"
     gene            618..1838
                     /locus_tag="VF036_02615"
     CDS             618..1838
                     /locus_tag="VF036_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX6264154.1"
                     /translation="MSGYARGPFGVDWEDRIDFPRLRSDRIAAAQQALRNSDIDSLLL
                     WKDENVRYLTSLRAQLIAGKMSSLNGVLLTRDAGPVLLCSGGEIDKASMGMSWLTAAH
                     PIPIMEQRELVDGFVKATLAPLLNELGVAAGRMGVDQVNYSVVESMQTHLPDLQPTDG
                     DHLMQQVRLIKSDDEIAIIEEACAIGDAVTQRAIDSSRSGRRENEVAGDAMQTLFYMG
                     GEMAHVITPFVASGEHMSPPHRICTDKIIRNGDLCFIDIGAMWNGYFADIGRTMMVGK
                     PSGMQKRVYTAVYRGLMAGVDVMRPGRTNQDVADAIISEVREHGFGDNLFSLFIGHGI
                     GMGANEPPYIGETLPGSTVFDLKPGMVFAVEPLVWVPDVQGGGGVRIEDMVLVTEDAP
                     RILSRVEYDDRLLD"
     gene            1858..>2425
                     /locus_tag="VF036_02620"
     CDS             1858..>2425
                     /locus_tag="VF036_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6264155.1"
                     /translation="MPSDPEATTDDRRAAGPRAGLSDRFWSRPHDQAPLLRRPAVYLL
                     AAATVAVLGLNWPIMSAGVDLVPPLWLAAFRMGGAAIVVAAVMATRGVPRPRAADRPI
                     LWSVGLARLALVTALVFSALEYVPPGRSSVLVYTASLWTVPLAVVFLHERLTSLRVAG
                     LALGSAGVVLLIEPWTLDWTDGDLLIGLG"
BASE COUNT          369 a          835 c          812 g          409 t
ORIGIN      
        1 aggtggaact gtgtgttcag ctcgacccag ttgccgagat cgcgttcctt cgccatccgc
       61 tcccgcaagc gctcggcctc ctggagctcc tcctccgtga tcttctccac cacgaggcgg
      121 accgccagcg gctcgagctg atggcggatc agacggatct cctccatctc ggaccagttc
      181 agctcccgga cgacgccgcc acggtgccgg tccagggtga tgaacccctc cgtggccagg
      241 tcccgcagcg cctctcgcac cggggtcgtg ctgacgttca gcatggtcgc caggtccgcc
      301 tggatcagcc gggacccgcc actcaggtcg ccccgcagga tggcccgtcg gagcgagtcc
      361 ttcacgaatt cgtgggcggt gcgcctccca cccgtcgtgg cgatactggc ccagtccacg
      421 tcgccggtta cagccttgtc cgttgccatg cacctctccc gctcgcgacc agcgcctctg
      481 ggcgccccgt tcgcacttcc gacaggatgt atgatacata cccgcccgga cagacccggg
      541 agcccgggac ctcgttctcg tcgtgggagc gaggcagaga gccagccccg ggtcaagggc
      601 agacccggag gagagtgatg agcggctacg cgcgcggacc attcggggtc gactgggagg
      661 atcggatcga cttcccccgc ctccggagcg accggatcgc ggctgcgcaa caggccctcc
      721 ggaactcgga catcgactcg ctcctcctct ggaaggacga gaacgtccgc tacctgacgt
      781 cgctgcgagc gcagctgatc gcgggcaaga tgagttccct caacggcgtg ctcctcacgc
      841 gtgacgcggg tccggtgctc ctgtgctcgg gcggtgagat cgacaaggca tcgatgggga
      901 tgtcgtggct gactgcggcg cacccgatcc ccatcatgga acagcgcgag ctcgtggatg
      961 gattcgtgaa ggcgacgctc gcgccgctcc tgaacgagct cggcgtggcg gccggccgga
     1021 tgggcgtcga ccaggtgaac tactcggtcg tcgagtcgat gcagacccat ctgccggatc
     1081 tccaacccac ggacggcgac cacctgatgc agcaagtacg cctcatcaag agcgacgacg
     1141 agatcgcgat catcgaggag gcctgcgcga tcggcgacgc ggtcacgcaa cgcgcgatcg
     1201 actcgagccg ctcgggacgt cgcgagaacg aggtggccgg cgacgcgatg cagaccctct
     1261 tctacatggg cggggagatg gcgcacgtga tcactccctt cgtcgcgtcg ggcgagcaca
     1321 tgtcgccgcc gcatcggatc tgcacggaca agatcatccg caacggcgac ctctgcttca
     1381 tcgacatcgg cgcgatgtgg aacggttact tcgccgatat cggcaggacg atgatggtcg
     1441 ggaagccctc cgggatgcag aagcgtgtct acacggcggt ctaccgaggg ctgatggccg
     1501 gcgtggacgt gatgcgtccg ggtcgcacga accaggacgt ggcggacgcg atcatctcgg
     1561 aggtccggga gcacggcttc ggcgacaacc tgttcagctt gttcatcggt cacgggatcg
     1621 ggatgggcgc gaacgagccc ccctacatcg gcgagacctt gccgggatcc acggtcttcg
     1681 acctcaaacc tgggatggtc ttcgcggtgg agccgctggt gtgggtaccg gacgtgcagg
     1741 gcggcggcgg cgtacggatc gaggacatgg tcctcgtgac cgaggacgcg ccccgcatcc
     1801 tgtcgagggt cgagtacgac gaccgtttgc tcgactagcc gaccgtcacc gcaaccgatg
     1861 ccgagcgatc cggaggcgac gacggacgac cgacgtgccg ccgggccccg ggcgggcttg
     1921 tccgaccgct tctggtcacg cccccacgac caggcaccgc tgttgcgccg gccggccgtc
     1981 tacctgctcg ccgccgccac cgtcgcggtg ctcgggctca actggccgat catgtccgcg
     2041 ggcgtggacc tcgtcccgcc tctgtggctc gccgcgttcc ggatgggggg cgcggctatc
     2101 gttgtcgccg ctgtgatggc aacgcgaggc gtgccgcgtc cgcgcgcagc cgatcgaccc
     2161 atcctgtgga gcgtcggcct cgcgcgcctc gcgctcgtga cggcgctcgt gttcagcgcc
     2221 ctcgagtacg tccccccggg aaggtcgtcg gtcctcgtct ataccgcgag cctgtggacg
     2281 gtcccgctgg ccgtcgtgtt cctccacgaa cgcctcacgt cgctccgcgt cgccggtctc
     2341 gcgctcggct cggccggcgt cgtcctgttg atcgagccgt ggacgctcga ttggaccgat
     2401 ggcgatctcc tgatcggtct cggca
//
LOCUS       DASUVE010000128         4577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_441511, whole genome shotgun sequence.
ACCESSION   DASUVE010000128 DASUVE010000000
VERSION     DASUVE010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441511"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VF036_02625"
     CDS             <1..712
                     /locus_tag="VF036_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6264156.1"
                     /translation="LPPILEEAGLRPDALELVFTGADHGIQGEVEQDYQWGFSLADIV
                     KDDVLLAYELNGQPLPPQHGYPIRLLVPDWYGMCSVKWLRRITAVAEPFDGFQLSAYQ
                     RRQEPDGPGERVTRKAVRALMVPPGFSDFPARQRFVDAGKHELIGRAWSGWGHIDRVE
                     VSTDGGASWSDGVLGPKPASPSSWRSWSFAWNAQPGMHELLVRATDEAGNVQPMDPAW
                     NYGGFMNNVPQRVPVVVR"
     gene            complement(716..1861)
                     /locus_tag="VF036_02630"
     CDS             complement(716..1861)
                     /locus_tag="VF036_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6264157.1"
                     /translation="MDLSSWRSEFPVLEHKAYLISASLGPVSQRSRAYLEEYLDAWAS
                     KGAPDHVWFEEIFPRMASLKRSFSMLAGCDPDEVALTTNISIALSTIASCLDLSGDRR
                     TVILSELDFPTDGHVWLAWARKTGAEIRWLRSSDGLTIPVEEYDRAIDERTALVMVNR
                     VLYRSSAIVDAKAVCAIARDRGALSFVDDYHGLGIVPLDLHDLGCDLYTAGVLKWMLG
                     GPGLVFLYARRDLLPSLEPAITGWFAQREPFSFDAEHLDYHPTARRLEHGTPPAPVFF
                     IAQGGIDIISEVGPAAIRVRNGELSDHVIARADGAGLEVRTPRDRDARGGVVNVKVGP
                     EAEKICHQLLARDVCTDYRGDGLRISPHFFNTEEDVDRCFDELRRLV"
     gene            complement(1904..3895)
                     /locus_tag="VF036_02635"
     CDS             complement(1904..3895)
                     /locus_tag="VF036_02635"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX6264158.1"
                     /translation="MERDLRTSPLYQEVEEHFRKVYEPGFGRVTEPSDPRPSPDGRWV
                     AFTGSIWEKLEGTAKTRICLAAAPGGAEGGVAGTKELSSGPEHDAGPRWSPEGSTLTF
                     LSDRREKGRMQLFSLSAGGLGEAEALPEIDGTIEEHAWSPDGSRILVRTAGMHADGAG
                     ADGSGALESDRDLPDWVPAVDSWEDRQVWRRLWVVDVATGEVRLLSREGLNVWEADWC
                     GDDAVVAVASEDPGESAWYDSPLVLIDVATGADRVIATSDVQFGLPVGSLDGTRVAAV
                     EAPCSDRQLVSGRAVVIDVASDARRAIEIEDADVTWLGWRANGGLAYTMLRRGDTVFG
                     EIDAGTGERTETWSTSESVGSWAPYAAVVGEDGFAFVRQGFDRPAEVAIVQQGADGTV
                     ASFRHEGHDHARGLIGRAERLTWTASDGLEIDGFLLAPAGEGPHPLILYVHGGPVWSY
                     AEWFPRPYLAWLVARGFAILMPNPRGSTGRGRAFLEAVIGDMGGGDAHDDLAGVDAVI
                     ERGFADPDRIAVTGGSYGGYMSCWLPVVDQRFKAAVAMSPVTDFYSEHWNSNIGAWDA
                     WFLGGEPQDGTTQYQERSPVFLADRVRTPTFLTAGTEDRCTPPGQAIEFYRALCANGV
                     PAEVAIYPGEGHGVRKLPAFLDLVTRSTAWFERFMPARS"
     gene            3995..>4577
                     /locus_tag="VF036_02640"
     CDS             3995..>4577
                     /locus_tag="VF036_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX6264159.1"
                     /translation="MGSERLVIHGAREHNLKNVTLDLPRDQLIVFTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSSYARQFLGQMEKPDVDFIEGLSPAISIDQKSTSRNPRSTVGT
                     ITEVYDYLRVLFARIGHPHCFNCGRPIGRQTPDQIVDQVLQLPEGTRFQVLAPVIRGR
                     KGEYEKHLQELARKGFPRARIDGEVRDLSEPIRL"
BASE COUNT          725 a         1676 c         1513 g          663 t
ORIGIN      
        1 gctgcctccc atcctcgagg aggccggatt gcgacccgac gccctcgagc tcgtcttcac
       61 gggcgcggac cacgggatcc agggagaggt cgagcaggac taccagtggg gcttctcgct
      121 cgccgacatc gtgaaggacg acgtgctgct ggcctacgaa ctgaacgggc agccgcttcc
      181 gccgcagcac gggtacccga tccggctgct ggtgccggac tggtacggga tgtgcagcgt
      241 caagtggctc cgccggatca cggccgtcgc cgagcccttc gacggcttcc agctctccgc
      301 gtaccagcgc cgtcaggagc cggacggccc cggcgagcgc gtgacgagga aggccgtccg
      361 agcgctcatg gtgccgccgg gcttcagcga cttccccgcg aggcagcggt tcgtcgacgc
      421 ggggaagcac gagctgatcg gccgcgcctg gtccggatgg gggcacatcg accgcgtcga
      481 ggtgagcacg gacggaggcg cgtcctggtc ggatggcgtt ctcggcccga agcccgcatc
      541 gccatcttcg tggcgctcgt ggtccttcgc ctggaacgcg caaccgggca tgcacgagct
      601 cctcgtccgg gcaacggacg aggctggcaa cgtccagccg atggacccgg cgtggaacta
      661 cgggggcttc atgaacaacg ttccccaacg ggtccccgtc gtggttcgct gacggtcaga
      721 cgagccgccg gagctcgtcg aagcagcggt cgacgtcctc ctcggtgttg aagaagtgcg
      781 ggctgatccg cagaccgtcg ccgcggtagt ccgtgcagac gtcccgtgcc aggagctggt
      841 ggcagatctt ctccgcctcg ggcccgacct tcacgttcac gacgccgcct cgggcgtcgc
      901 ggtcccgcgg cgtgcgcacc tcgagacccg ctccgtccgc ccgggcgatg acgtgatcgg
      961 agagctcgcc gttccggacg cggatcgcgg ccggtcccac ctccgagatg atgtcgatcc
     1021 cgccctgcgc gatgaagaac acgggcgccg ggggcgtgcc gtgctccagg cggcgcgccg
     1081 tggggtgata atcgagatgc tccgcatcga aggagaacgg ctcccgttgc gcgaaccacc
     1141 ccgtgatggc gggctcgagc gaggggagca gatcgcgccg ggcatagagg aacacgagcc
     1201 ccggtccgcc gagcatccac ttgaggacac cggcggtgta gaggtcgcag ccgaggtcgt
     1261 ggagatccag tggaacgatc ccgaggccgt ggtagtcgtc cacgaacgag agcgccccgc
     1321 gatcgcgggc gatcgcgcag actgccttgg cgtcgacgat cgcgctcgac cggtagagga
     1381 cccgattcac catcacgagc gcggttcgct cgtcgatcgc gcggtcgtac tcctcgaccg
     1441 ggatcgtgag cccgtcggag ctgcggagcc atcggatctc ggcgcccgtc ttccgcgccc
     1501 atgcgagcca gacgtgccca tcggtgggga agtcgagctc cgacaggatc actgtcctcc
     1561 ggtcgccgga caggtcgagg caggaggcga tcgtcgacag cgcgatcgag atgttcgtgg
     1621 tcagcgcgac ctcatcggga tcgcaaccgg cgagcatcga gaacgagcgc ttcagcgacg
     1681 ccatccgggg gaagatctcc tcgaaccaga catgatcggg cgcccctttc gaggcccacg
     1741 cgtccaggta ctcctcgagg tacgccctcg accgctggga gacgggaccg agcgacgcgc
     1801 tgatcaggta ggccttgtgc tcgaggacgg ggaactcgct tcgccaggaa ctcaggtcca
     1861 cggggatccc ctcatccggt ccccgaccgc gcgtgcgcgg gactcacgac ctcgccggca
     1921 tgaagcgctc gaaccacgcc gtcgatcgtg tgacaaggtc gaggaacgcc gggagcttcc
     1981 gcacgccgtg tccctcgccc gggtagatcg cgacctcggc cggcacgccg ttcgcgcaga
     2041 gggcacggta gaactcgatc gcctggccgg gcggtgtgca ccgatcctcg gtacccgccg
     2101 tcaggaacgt cggggtcctc actcgatccg cgaggaagac gggactgcgc tcctggtact
     2161 gcgtcgtccc gtcctgcggc tcgccaccga ggaaccacgc atcccaggcg ccgatgttgc
     2221 tgttccagtg ctcgctgtag aagtcggtga cgggggacat cgcgaccgcc gccttgaacc
     2281 gctggtccac caccggaagc cagcacgaca tgtacccgcc gtagctcccg cccgtcaccg
     2341 cgatccgatc cggatcggcg aagccgcgct cgatcacggc gtcgacgccc gcgaggtcat
     2401 cgtgggcgtc gcccccgccc atgtcgccga tcacggcctc caggaacgcc cggccgcgcc
     2461 ccgtggaacc acgcgggttc ggcatcagga tcgcgaaccc gcgcgctacg agccaggcga
     2521 ggtacggcct cgggaaccac tccgcgtagg accagaccgg cccgccgtgc acgtacagga
     2581 tcagcggatg cggtccctcg cctgcagggg cgagcaggaa tccgtcgatc tcgagaccat
     2641 cggacgccgt ccaggtgagc cgctcggctc gcccgatgag accccgggcg tgatcgtggc
     2701 cctcgtgccg gaacgacgcg accgtgccgt cggcaccctg ctggacgatc gccacctcgg
     2761 cgggacgatc gaacccctgt cggacgaacg cgaacccgtc ctcgccgacg acggccgcgt
     2821 acggcgccca cgagcccacg ctctcgctcg tggaccacgt ctccgtgcgc tcgcccgtcc
     2881 cggcatcgat ctcaccgaac accgtgtcgc cccgccggag catcgtgtag gcgaggcccc
     2941 cgttggctcg ccaacccagc cacgtgacgt cggcgtcctc gatctcgatc gcgcggcgtg
     3001 catccgaggc cacatcgatc acgaccgccc gaccggacac gagctggcgg tcgctgcagg
     3061 gcgcctccac cgcggccacc cgcgtcccat cgagcgagcc gacggggagc ccgaactgca
     3121 cgtcgctggt cgcgatcact cggtccgcgc cggtcgccac gtcgatcagg acgagcgggg
     3181 agtcgtacca cgccgactcg ccgggatcct cggacgccac cgcgacgacg gcgtcgtcac
     3241 cgcaccagtc cgcctcccag acgttcagtc cttcccgcga cagcagccga acctcgccgg
     3301 tcgcgacgtc gaccacccag aggcgtctcc agacctgccg gtcctcccat gagtccaccg
     3361 ccgggaccca gtcgggcaga tcacgatccg actccagcgc gcccgacccg tcggcacccg
     3421 cgccgtcggc atgcatcccg gccgtccgga cgaggatccg ggacccgtcc ggcgaccacg
     3481 cgtgctcctc gatcgtgcca tcgatctccg gtagcgcctc ggcctcgccg aggccgccgg
     3541 cagacaggga gaacagctgc atccggccct tctcgcgccg atccgagagg aacgtgagcg
     3601 tgctcccctc cggcgaccac ctggggccgg catcgtgctc gggtcccgag gagagctctt
     3661 tcgtgccggc caccccgccc tcagcgccac ccggcgccgc cgccaggcag atccgggtct
     3721 tcgccgtccc ctcgagcttc tcccagatgg acccggtgaa cgccacccac cgcccgtcgg
     3781 gcgaggggcg cgggtccgat ggctcggtca ctcgaccgaa tcccggctcg tagaccttcc
     3841 ggaagtgctc ctcgacctct tggtacagcg gcgacgtgcg caggtccctc tccatgggca
     3901 caggataggc cgaggaagcg cccatgctcg agcggctccc ctcgagtccg gcgccgagaa
     3961 caggcgtgtc gcgggccgcc ggtacactcg acccgtgggc tccgagagac tcgtcatcca
     4021 cggcgcccgt gagcacaacc tcaagaacgt cacgctcgac ctgccgcgtg accagctgat
     4081 cgtgttcacg gggctgtcag gctccgggaa gtcgtccttg gccttcgaca cgatctacgc
     4141 cgagggccaa cggcgctacg tcgagtccct gtcgtcgtac gcccgacagt tcctcgggca
     4201 gatggagaag cccgacgtcg acttcatcga ggggctctcc ccggcgatct cgatcgacca
     4261 gaagtccacg tcacgcaacc cgcggtccac ggtcggtacg atcacggagg tctacgacta
     4321 cctccgcgtg ctcttcgcgc gcatcggaca tccccattgc ttcaactgcg gccgtccgat
     4381 cggccggcag acgcccgacc agatcgtgga tcaggtgctc cagctgcccg agggcacccg
     4441 cttccaggtc ctcgctccgg tgatccgtgg tcgcaagggc gagtacgaga agcacctcca
     4501 ggagctcgcg cgcaagggct tccctcgcgc gcggatcgac ggcgaggtcc gcgacctgag
     4561 cgagccgatc cggctgc
//
LOCUS       DASUVE010000129         3549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1382746, whole genome shotgun sequence.
ACCESSION   DASUVE010000129 DASUVE010000000
VERSION     DASUVE010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382746"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VF036_02645"
     CDS             <1..479
                     /locus_tag="VF036_02645"
                     /inference="COORDINATES: protein motif:HMM:NF016310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HEX6264160.1"
                     /translation="PTWDAVAGLGAREEVVTLLDLRTARDELSHVPDGAEIGAVSVGT
                     PHASESELRRLAELVAEAHLRVPFYVNTGRVQLAAIGEITGELADAGVTVVTDTCTYI
                     TPVIREVTAPVMTDSGKWAWYAPSNLGVDVALGSLEDCVRSAAAGVVIRDEAPWAG"
     gene            479..862
                     /locus_tag="VF036_02650"
     CDS             479..862
                     /locus_tag="VF036_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HEX6264161.1"
                     /translation="MDGRFLVEGHVDGTALVLAEPLSFWGGLDPVTGEIVEANHPQRG
                     EVVTGRVLVMPSGRGSSSSATVLAESIRLGTGPAGVVLGEADEIVAIGALVAEVLYGV
                     TTAVFVTSRDGYSAIRHGDHLAFVP"
     gene            complement(859..1977)
                     /locus_tag="VF036_02655"
     CDS             complement(859..1977)
                     /locus_tag="VF036_02655"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX6264162.1"
                     /translation="MTAQLLSAPRPRSVLEVVERLGSVQMDPTRTVARTEHLVLWSRL
                     GRRFRVEELERLLWRDRSLFEYRAFILPTSQFAVHRETMRRYPGPSNGRHAYVHRYLR
                     ENPRFRRYVLQRLRDEGPLPTRAFEDRSAVGWRTGGWNDDGRNTSMMLEVLWAKGEVM
                     IAGRVGQERVWDLARRSLPFDEPRPSAGEEARSLLDVQLRALGISKVNRFGWTFAGER
                     PPGWERALARLEREGTAVRVTVDGLPGERWAHREVLDRSYRGRTVLLSPFDRLIHDRS
                     RAEELFGFRFRLEIYVPKAKREYGYFVFPILHGDHVVGRLDPVHDRAANMLRVQGVFA
                     EDDAPPDAWPAIRKQLDELAAWVGADDVELPSLPRVWR"
     gene            complement(2028..2423)
                     /locus_tag="VF036_02660"
     CDS             complement(2028..2423)
                     /locus_tag="VF036_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6264163.1"
                     /translation="MIGWVGDIERMTLDNDTFRTVLFTGAHTQLTVMRLTPGEDIGKE
                     VHPDRDQFIRIEEGQARVEFGRDEDGVDESHDVSDDWAIVIPSGTWHNVINTGPGELK
                     LYSLYSPPEHPDGTVHRSKADAEAAEHAG"
     gene            complement(2516..2867)
                     /gene="ssrA"
                     /locus_tag="VF036_02665"
     tmRNA           complement(2516..2867)
                     /gene="ssrA"
                     /locus_tag="VF036_02665"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2925..>3549)
                     /locus_tag="VF036_02670"
     CDS             complement(2925..>3549)
                     /locus_tag="VF036_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264164.1"
                     /translation="VGERRRAPTDERTRGMRKRMTMVLAALGILMTGACSGSDGASNG
                     GAGGETVSAEAYATGVCGAIADWVDEIQGLNENLQANLDPTSIDALKDAMVTFLDDVI
                     ASTDTVIADVEDVGIPDVDDGDAAAQHLLTGLRDSRGVFEDARDRVEAMPTDDPAAFG
                     EELQTVGTDIQTSMSTIGEDLGRFESPVLDDVSKEIPECEAVAASAS"
BASE COUNT          515 a         1291 c         1174 g          569 t
ORIGIN      
        1 cgccgacgtg ggacgccgtg gcggggctcg gcgctcgcga ggaggtggtc accctcctcg
       61 acctccgtac cgcgcgcgac gagctctcgc acgtgccgga cggcgcggag atcggcgccg
      121 tgagcgtggg aacgccccac gcgtccgaat ccgagcttcg ccggctcgcg gagctcgtgg
      181 ccgaggctca cctgcgcgtc cccttctacg tgaacacggg ccgtgtgcag ctcgccgcga
      241 tcggcgagat caccggcgag ctcgccgacg ccggcgtcac ggtcgtgacc gatacgtgca
      301 cgtacatcac gccggtgatc cgcgaggtca ccgctcccgt catgaccgac tccggcaagt
      361 gggcgtggta cgcgccgagc aatctcggcg tcgacgtcgc cctcggtagc ctcgaggact
      421 gcgtgcggtc ggcggcggcc ggggtcgtga tccgcgacga agcgccctgg gccggctgat
      481 ggacggccga ttcctcgtcg agggacacgt cgacgggacc gcgctcgtcc tcgccgagcc
      541 gctgagcttc tggggtggac tcgatcccgt gacgggtgag atcgtcgagg cgaaccatcc
      601 ccaacggggt gaggtcgtca ccggtcgagt cctcgtgatg ccgtcgggac gcggctcgtc
      661 ttcgagcgcc acggtcctcg cggagtcgat ccggttggga acgggccccg ccggggtcgt
      721 cctcggggaa gccgacgaga tcgtcgcgat cggggcactg gtggcggagg tgctctacgg
      781 cgtcacgacc gcggtgttcg tgacgtcccg ggacggctac tcggcgatcc gccacggcga
      841 ccacctcgcg ttcgtgccct agcgccacac gcgagggagc gacggcagct ccacgtcgtc
      901 ggcgccgacc catgcggcga gctcgtcgag ttgcttccgg atcgccggcc acgcgtcggg
      961 cggcgcgtca tcctcagcga acacgccctg cacccgcagc atgttcgcgg cccgatcgtg
     1021 gacgggatcg aggcgtccga cgacgtgatc gccgtgcaag atcgggaaca cgaagtaccc
     1081 gtactcgcgc ttcgccttcg ggacgtagat ctccaggcgg aaccggaacc cgaagagctc
     1141 ctcggcgcgc gagcggtcgt ggatcaatcg gtcgaacggc gacagcagga ccgtccggcc
     1201 tcggtacgag cgatcgagca cctcgcgatg cgcccaccgc tcgcccggga gcccgtctac
     1261 cgtcacgcgg acggcagttc cctcccgctc gagccgggcg agtgcccgct cccatcccgg
     1321 gggccgttcg cccgcgaacg tccacccgaa ccggttgacc ttggagatgc cgagggcccg
     1381 cagctgcacg tcgaggagac tccgggcctc ctctcccgca ctcggccggg gttcgtcgaa
     1441 cgggaggctc cgcctcgcga gatcccagac gcgctcctgg ccgaccctgc ccgcgatcat
     1501 gacctctccc ttggcccaca ggacctcgag catcatcgag gtgttcctgc cgtcgtcgtt
     1561 ccacccgccg gtccgccaac cgaccgcgga gcgatcctcg aatgcccggg tgggcagggg
     1621 cccctcgtcg cgcagccgct gcaagacgta ccgtcggaac cgcgggttct cgcggaggta
     1681 ccggtggacg tacgcgtgcc gcccgttgct gggacccggg tagcgccgca tggtctcccg
     1741 atgcacggcg aactgcgagg tcgggaggat gaacgcccgg tactcgaaca gcgaccgatc
     1801 ccgccagagc agccgctcga gctcctcgac ccggaaccgt cggccgagcc gtgaccacag
     1861 gacgaggtgc tcggtccgcg ccaccgtccg cgtcggatcc atctgcacgc tgcccagccg
     1921 ctcgacgacc tcgaggaccg accgcggccg cggcgccgac agcaactgcg cggtcacggc
     1981 caggcgccgc gcccgctgga ccgatatcga acgaggttcg gccgagctca cccggcgtgc
     2041 tcggccgcct cggcgtccgc cttcgagcga tggacggtac cgtcgggatg ctccggcggt
     2101 gagtacagcg aatacagctt cagctcaccc gggcccgtgt tgatcacgtt gtgccaggtt
     2161 ccgctcggga tcacgatcgc ccagtcgtcg gagacgtcgt gcgactcatc gaccccgtcc
     2221 tcgtcccggc cgaactcgac gcgagcctgt ccctcctcga tccgaatgaa ctggtcgcgg
     2281 tccgggtgca cctctttgcc gatgtcctcg cccggggtga ggcgcatcac ggtgagctgc
     2341 gtgtgggcgc ccgtgaacag caccgtccgg aacgtgtcgt tgtcgagcgt cattcgttcg
     2401 atgtctccga cccaaccgat catcgagggc tcctcccaag tcgtgagagc cggagcgtaa
     2461 cgaacgaagg ccttccggaa agaaggcctt cgtcgcgtct gcagcacaac ggatatcgtg
     2521 gaggtggcgg gattcgaacc cgcgtcgcag ggcgaccgtc tgggacttct ccgggcgcag
     2581 ccggtgggta gatctcacct cggggctccc accggcggag gacccctcgg ccagcccggg
     2641 tgcgatgtcc ctcgggggcc cccgggcgga gccccctcgg tgagtcctgc ttacgatgcc
     2701 ctgtccgggc cgcaggccct cccggacgga cagctaccta gtgactaggc agcgagcgcg
     2761 tactcatcgt tggcgcttgt gttggttccg gcgtttttcg agactccgga gatctcggcc
     2821 cgcttctccc aggcgataca cgccctgctc gaagccagta cacccccata tgtagttgtg
     2881 ttccccagta tacgagcggc ggtggccacg ccccgaacga gcgcctatga cgccgatgcg
     2941 gccaccgctt cgcactccgg tatctctttc gacacgtcgt cgagcacggg tgactcgaac
     3001 cggccgagat cctcgccgat cgtggacatc gaggtctgga tatcggtccc gacggtctgg
     3061 agctcctcgc cgaacgccgc cgggtcgtcc gtcggcatcg cctcgacacg gtcgcgcgcg
     3121 tcctcgaaca cgcctcgaga gtcgcgcaac cccgtgagca ggtgctgggc cgcggcatcg
     3181 ccgtcgtcga cgtccggtat cccgacgtcc tccacgtcgg cgatcaccgt gtcggtcgag
     3241 gcgatcacgt cgtcgaggaa cgtgaccatc gcgtccttca gcgcatcgat gctcgtcgga
     3301 tcgaggttcg cttgcaggtt ttcgttcagg ccctggatct cgtcgaccca gtccgcgatc
     3361 gcgccgcaca cgccggtcgc gtaggcctcg gccgacacgg tctccccgcc cgctccgccg
     3421 ttcgacgctc cgtcggatcc cgagcacgcg cccgtcatga ggatccccag agctgccaac
     3481 accatcgtca tccgcttccg catcccacgc gtcctctcgt cggtcggcgc tcgccgccgc
     3541 tccccaacc
//
LOCUS       DASUVE010000130         4123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_629502, whole genome shotgun sequence.
ACCESSION   DASUVE010000130 DASUVE010000000
VERSION     DASUVE010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629502"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VF036_02675"
     CDS             complement(<1..987)
                     /locus_tag="VF036_02675"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX6264165.1"
                     /translation="MEESEERYRLPRTVVPSRYDLTLEPDLDAGTFRGSVDVSLTVLG
                     SVTEIVLNANELRLEEAALTRADGGRSVEVAKILTDDDAERATIELAEAAEPGDWTLR
                     IVFAGELNPRLTGFYRSTYTDGDGRTQVVGTTHFEATDARRAFPCWDEPDLKAVFAST
                     LVVKEGLTAVSNGPEAEREALGDGRMRIRFADTMKMSTYLAAWVVGPLEITEPADAGG
                     VPVRIVHVPGKGALTTFAVDSSVHSLNWFADYYGIPYPEANLDNIALPDFAQGAMENV
                     GCVTYREQVLLIDPARSTQEEQLGVAETVAHELAHMWFGDLVTMRWWNGIWLN"
     gene            complement(987..1913)
                     /locus_tag="VF036_02680"
     CDS             complement(987..1913)
                     /locus_tag="VF036_02680"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX6264166.1"
                     /translation="MPITQVEKIWMDGELVPWQDAQVHVLSHAVHYGSGVFEGIRAYE
                     TARGAAVWHLDEHLKRLFRSAKLYHTDIPYSREAITQATKDVIRANELNACYIRPLVL
                     RGYGEMGVNPLQAPVNVVIAVWPWGAYLGEDALEEGVRIKISSWRRNSQNSLPAAAKA
                     TGQYINSVLAKVESLKAGYDEAIMLNEAGFVTDGSGENVFVVRDGVLTTPPISAGCLD
                     GITRGSTITLARDLGYEVHEENLVRTDLYNADECFFTGTAAELTPIREVDDRVVGEGH
                     RGPVTKELQGAFFAATKGENEKYVHWLTYVND"
     gene            complement(1946..2728)
                     /locus_tag="VF036_02685"
     CDS             complement(1946..2728)
                     /locus_tag="VF036_02685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6264167.1"
                     /translation="MRFDRAVEYYDRTRGLSEEASREMTVLLSSELRGRGRTLEIGVG
                     TGLVALPLAAAGVPLVGLDLSAPMLAKLVEKAGGRAPFPLVVGDATTLPFDEHRFGAA
                     VVRHVLHLVPAWRTVVAELVRVVAPGGLVLVSSGQVPDPWHELTDRFMDRVGRPSFSH
                     ALDSWETGAIVHAFRAHGAETRQLPWITERVAQTVGEFLDQMAAGMHSWTWEVDEEER
                     REAVAELRLWAVERWGSLDPPGGRDVSIEWHAFDLHRRRRVS"
     gene            complement(2737..3888)
                     /locus_tag="VF036_02690"
     CDS             complement(2737..3888)
                     /locus_tag="VF036_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX6264168.1"
                     /translation="MSETFTFYIQPWYRKSPYFEATKRAGCASWGLYNHMLLPTLYDD
                     PVTEYWALLRDVTMWDVSVERCVEISGPDALPFTNLLTCRDLTSCEVGQCKYVLVTDR
                     DGGIVNDPVLLRIAEDRFWLALADSDALLYVKGVAALAGMDVRVIEADVSPMQIQGPR
                     SRDVVRDLVGDEVAALRYYRCAEAEIDGIPLVVSRTGWTGEVGYELYLCDTSRGDELW
                     RLVEEAGAPYAIRAIAPSEARRIEAGIFNYGSDMRTEDTPFHVTGLERLVEEQDAEFI
                     GKDALRRIAEEGVDRKLVGIDIQGAPMNDEGALNDFWPVHEPGGDRIGRVTAGAWSPR
                     LERNIGYAWVPATHIAVGTALALHAHDGTREATVATLPFVDPTKEIPKS"
BASE COUNT          647 a         1473 c         1349 g          654 t
ORIGIN      
        1 gttcagccag atcccgttcc accagcgcat cgtcacgagg tcgccgaacc acatgtgcgc
       61 gagctcgtgg gccacggtct ccgccacgcc gagctgctcc tcctgggtcg accgcgccgg
      121 gtcgatcaac agcacctgct cccggtacgt cacgcacccg acgttctcca tcgcgccctg
      181 cgcgaagtcc gggagcgcga tgttgtcgag attggcctcc gggtacggga tgccgtagta
      241 gtccgcgaac cagttcagcg agtggacgct cgaatcgacc gcgaaggtcg tgagcgctcc
      301 cttgccgggc acatggacga tccgcaccgg cacgccaccc gcgtcggcgg gctcggtgat
      361 ctcgagcgga ccgacgaccc aggcggccag gtacgtcgac atcttcatcg tgtcggcgaa
      421 gcggatccgc atccgtccgt cgccgagggc ctcccgttcc gcctccgggc cgttcgagac
      481 ggcggtcagc ccttccttca cgacgagcgt ggacgcgaag acggccttga ggtccggctc
      541 gtcccagcac gggaacgcac gccgcgcgtc cgtcgcctcg aagtgcgtgg tcccgaccac
      601 ctgcgtgcgg ccatccccgt cggtgtacgt gctccggtag aagccggtga ggcgcgggtt
      661 cagctcgccc gcgaacacga tccggagcgt ccagtccccc ggctccgcgg cctccgcgag
      721 ctcgatggtc gctcgctcgg cgtcgtcatc ggtgaggatc ttggcgacct ccaccgagcg
      781 gccgccgtcc gcccgggtga gcgcggcctc ctcgagccgc agctcgttcg cgttcaggac
      841 gatctcggtg accgatccga ggaccgtgag cgagacgtcg acgctcccgc ggaacgtccc
      901 ggcgtcgagg tcgggctcca gcgtcaggtc gtagcgcgag gggacgaccg tccgaggcag
      961 ccggtatcgc tcttcggact cctccatcag tcgttcacgt aggtcagcca gtggacgtac
     1021 ttctcgttct cgcccttggt ggctgcgaag aacgcgccct gcagctcctt ggtcaccgga
     1081 ccccggtggc cttcacccac gacgcggtcg tcaacctcac ggatcggcgt gagctccgcg
     1141 gccgtacccg tgaagaagca ttcatcggcg ttgtagagat ccgtgcggac gaggttctcc
     1201 tcgtggacct cgtagccgag atcgcgagcg agcgtgatcg tggagccccg cgtgatgccg
     1261 tccaggcacc cggccgagat cggcggagtg gtgagcacgc cgtcccgcac cacgaacacg
     1321 ttctcgccgg agccgtccgt cacgaagccg gcctcgttca gcatgatcgc ttcgtcgtag
     1381 ccggccttga ggctctccac cttggcgagg acggagttga tgtactgacc ggtggccttc
     1441 gccgcggcgg gcagcgagtt ctgtgagttg cgacgccagc tgctgatctt gatgcgcacg
     1501 ccctcctcga gggcgtcctc accgaggtag gcaccccacg gccacacggc gatcacgacg
     1561 ttcaccggag cctgcagtgg gttcacgccc atctcgccgt agccacggag cacgagcggc
     1621 cggatgtagc acgcgttcag ctcgttcgcg cggatcacat ccttggtggc ctgcgtgatc
     1681 gcctcgcgcg agtacgggat gtcggtgtgg tacagcttcg ccgagcggaa cagtcgcttc
     1741 aggtgctcgt cgaggtgcca gacggccgcc ccccgggccg tctcgtacgc tcggatcccc
     1801 tcgaacacgc cgctgccgta gtgcacggcg tgcgacagca cgtgcacctg agcgtcctgc
     1861 cacggcacca gctcgccgtc catccagatc ttctcgacct gcgtgatcgg cacctgagtc
     1921 ctccttgacg acgttcggcg gcctttcagg atacccggcg ccttcgatga aggtcgaacg
     1981 cgtgccactc gatcgagacg tcacggcctc cgggcggatc cagcgatccc cagcgctcca
     2041 cggcccagag gcggagctcg gccaccgcct cccgtcgctc ctcctcatcg acctcccacg
     2101 tccacgagtg catacccgcg gccatctgat cgaggaactc ccccaccgtc tgagcgaccc
     2161 gttcggtgat ccatgggagc tgccgagtct cggctccgtg cgcccggaac gcgtgcacga
     2221 tcgcgccggt ctcccacgaa tcgagcgcgt gggagaagga ggggcggccg acccggtcca
     2281 tgaaccggtc ggtcagctcg tgccacggat cggggacctg accgctcgag accagcacca
     2341 gcccgcccgg tgccaccacg cggacgagct ccgccacgac cgttcgccag gccggcacca
     2401 gatggaggac gtgacggacc actgccgcac cgaagcggtg ctcgtcgaac ggcagtgtcg
     2461 tggcgtcccc gacgacgagc gggaacggtg ctcgcccacc cgccttctcg acgagcttgg
     2521 cgagcatcgg tgcggacaga tcgagaccga cgagcggcac ccccgccgcc gcgagcggca
     2581 gcgcgacgag tccggtgccc acgccgatct cgagggttcg accgcgaccg cggagctcgc
     2641 tcgagaggag cacggtcatc tccctcgaag cctcctcgga caggccccgc gtgcggtcgt
     2701 agtactcgac cgcccgatcg aagcgcatcg acccgctcac gacttcggga tctccttcgt
     2761 cgggtcgacg aacggcagtg tcgccacggt cgcctcgcgc gtcccgtcgt gggcatgcag
     2821 cgccagagcc gtgccgacgg cgatgtgcgt cgccgggacc caggcgtagc cgatgttgcg
     2881 ctccagccga ggcgaccaag cgccggcggt gacccggccg atccggtccc cgcccggctc
     2941 gtggaccggc cagaagtcgt tcaaggcgcc ctcgtcgttc atcggcgcgc cttggatatc
     3001 gatcccgacg agcttccgat ccaccccctc ctcggcgatc cggcgcaacg cgtccttccc
     3061 gatgaattcg gcgtcctgct cctcgacgag ccgctcgagc ccggtgacgt ggaacggcgt
     3121 gtcctccgtg cgcatgtcgg acccgtagtt gaagatcccc gcctcgatcc gccgcgcctc
     3181 tgaaggagcg atcgcgcgga tcgcgtacgg cgcgccggcc tcctccacga ggcgccacag
     3241 ctcgtcgccg cgtgacgtgt cgcagaggta gagctcgtag cccacctctc ccgtccaccc
     3301 cgtccgggag acgacgagag ggataccgtc gatctctgct tcggcgcacc ggtagtaccg
     3361 gagcgcagcg acctcgtcgc cgacgagatc gcgcacgacg tccctcgagc gcgggccctg
     3421 gatctgcatc ggcgagacgt ccgcctcgat cacgcggaca tccatcccgg cgagggctgc
     3481 cacgcccttg acgtagagga gcgcatcgga gtccgcgagc gcgagccaga accgatcctc
     3541 ggcgatacgg agcaggaccg gatcgttcac gatgccgccg tcgcgatccg tgacgaggac
     3601 gtacttgcac tgccccacct cgcacgaggt gaggtcccgg cacgtgagga ggtttgtgaa
     3661 cgggagcgcg tccggccccg agatctcgac gcaccgctcg accgagacgt cccacatcgt
     3721 cacgtcgcga aggagcgccc agtattcggt caccgggtcg tcgtagagcg tcgggagcag
     3781 catgtggttg tacagccccc agctcgcaca cccggcacgt ttggtggctt cgaaatacgg
     3841 ggacttccgg taccagggct ggatgtagaa cgtgaacgtc tccgacatcg aggctcctcg
     3901 tgaagggtcc gccggagcct acgatgcgat ggacgtcaag gacgtggcgc gtcggtccga
     3961 tggggggatc ggaggttctg caccgtgaca cccatgagcg cgaagaggtc ggaggccgac
     4021 gcggcgaccg atcacgaggt cgaactgccc ttccgggttc tgatcccgag aggcgatccc
     4081 caaggtcgtg ccggagagcg cgatgccgag cgaacgcccg tga
//
LOCUS       DASUVE010000131         7731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_253417, whole genome shotgun sequence.
ACCESSION   DASUVE010000131 DASUVE010000000
VERSION     DASUVE010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253417"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VF036_02695"
     CDS             <1..707
                     /locus_tag="VF036_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6264169.1"
                     /translation="VLVTGGSVDRMSNAPLAHELASDFTVLNYDRRGRGPSGDTPPYA
                     IEREIEDIEAMLEAAGGSANLYGSSSGAALAFQATAAGLPVTKLALWEPPYIIDENAR
                     PPKDQVEQYETMVAEGRRGDAAEYFMTKVVGMPPEFAAQARNEPWWPNQEALAHTLAY
                     DARIMGDYSVPTDQAASVKVPTLVVAGGADMPFMRETAQALADALPDGQVRLLDGQGH
                     NVDPTVLAPALKGFFE"
     gene            718..1167
                     /locus_tag="VF036_02700"
     CDS             718..1167
                     /locus_tag="VF036_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX6264170.1"
                     /translation="MRFRTTLEQAGKTATGFEVPSTVVEGLGAGKRPAVRVTINGFTY
                     RNTIASMGGRFMVGVSAENRAGAKVAGGDVIDVDIELDTAPRVVTMPKDFAAALRKEP
                     KAKATFDGLSNSNKGWHISSIEGAKTDETRQRRIAKSVAALREGKPR"
     gene            1197..1973
                     /locus_tag="VF036_02705"
     CDS             1197..1973
                     /locus_tag="VF036_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264171.1"
                     /translation="MVEEETPEPVDRDEERARIADLLAVHAPPDWEERFEREQLLDAV
                     IDTTPLQMFVMAADIRESTLLMKEAVEFERFAKIMDKFVSAVRSGIGTPGGWFDKFTG
                     DGFLAYWIAPSTPKEEYQRRFVEAAGNLAHTAHELIELFHRRVLEDFRRNSRNLSDGV
                     GLAIGLDAGPGFLVEIAGELTLVGPPVVGAVRMVNAASRPQEIFANVYLGEHLHDEQD
                     GIYEALGMTATRESRPTKEYPKGQEVYALTFRDAEQPEGG"
     gene            complement(1983..2900)
                     /locus_tag="VF036_02710"
     CDS             complement(1983..2900)
                     /locus_tag="VF036_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HEX6264172.1"
                     /translation="MTGQELHGVVVAGLGRGAELLADELVLDRIEEVFGLRVVPGTLN
                     VRLDASLDRSLTTRYVAASDIDPGWEAATGQAGYHLAHVLVAGRYRGIAFQADEPGYP
                     EDLVEIMCEVHLRSTPGLEDGDPISATVTPGGEPSSGSLARWRAAVGSTLFFALAPGI
                     VAGLLPWMITRWQVADPPPWPLRAFGAALLVAAVLVLVSAFARFVTEGLGTPAPVAPT
                     QRLVIGGLYRYVRNPMYLAVLAAIAGQALILGRIELVAYGFVVAVAFVAFVRVYEEPT
                     LRATFGDAYETYRRAVPGWWPRLRPWTPD"
     gene            2977..3852
                     /locus_tag="VF036_02715"
     CDS             2977..3852
                     /locus_tag="VF036_02715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011594647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6264173.1"
                     /translation="MFTRAEFGISITPDWSKQEEILDLAHAADDGGLDLIGIQDHPYQ
                     WRFHDTWSLIAFLAGQTSRIGFFPDVANLPLRPPATLAKSAASLDVLTGGRIELGLGA
                     GSFWEAIEAAGGPRRTPAEAVEAIEEAIDVMRLVWSGERGVRFEGKHYRLAGFHTGPK
                     PAHDIGIWVGAGRRRILDLIGRKADGWVPSSPWAPPEALPGYLRRIDEAAATAGRDPA
                     SIRRVYNLMGAIQPERRERLVGPVSYWVEELASLVDIGMDAFIFWPGTGDPLGQVELF
                     ASEVAPSVRASTGGP"
     gene            3870..4220
                     /locus_tag="VF036_02720"
     CDS             3870..4220
                     /locus_tag="VF036_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264174.1"
                     /translation="MRFGRFAFGSIEVDGTTYEHDLVIDRGGIRKRKKGPSKALRARY
                     GHTPLSLGEDIPWDCERLVIGSGAAGSLPVVDDVAEEAERRGVELLVLPTSEAIVELE
                     RAPGGSNAILHVTC"
     gene            complement(4239..5192)
                     /locus_tag="VF036_02725"
     CDS             complement(4239..5192)
                     /locus_tag="VF036_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6264175.1"
                     /translation="MDDGSPRDEEEALDAYSRVVTSVAERLIPSVASLRVQQRARGAS
                     VDGAGSAVVITPDGFALTSAHVVGGTNRGSASFVDGREVAFDVVGRDQLSDLAVLRLQ
                     GDGFASAELGEAAELRVGQLVVAIGNPMGFAGSVTAGVVSALGRALPVQAGRVVENVI
                     QTDAALNPGNSGGALADGRARVVGINTAIAGFGLGLAVPIDDSTRRIMHALMSAGRFQ
                     RAYIGIAGGGRPLPPRAAAAVGREEGVEIMEVVDGSPAARAGIRSGDLIVELDGAPIT
                     DARDLQRLMVGEVIGRSMEARVWRDGSLRSMAIHPVELTAA"
     gene            5294..6571
                     /locus_tag="VF036_02730"
     CDS             5294..6571
                     /locus_tag="VF036_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX6264176.1"
                     /translation="MSAVSTERHPRLLPVAEEARVTPIELFFDLVFVFSLTQVTALMG
                     DDLSWHGVARGILVLALLWWCWVGYAWLGNVVQAEEGLGKATMFASMAAMFVMALSVP
                     EAFDDLDGGLDGPVVIAFAYLAVRVLHLAIFWMVAGTSEDPGLRGQLIRFAPSLVGST
                     AILLVASQLSGTAQTLTWVGALLVDYVGVILGGASGWRLRSASHFAERHGLIVIVALG
                     ESIVAIGVGVSDLPISWPIIAAAVLGLVVAACLWWAYFDVVSIVAERVLRRLEGEERT
                     RLARDAYSYLHLPMVAGIALVARGIEEVMHQVGHATDGELSRTLDIVPLASLFGGAAL
                     FLFGHVGFKYRTWGSVTVRRLVVALLLCALIPLAAELPSLGALGLLAAILVTMIASEA
                     IRYAEEREQIRHEAVGPEVHREHREADQGMAER"
     gene            complement(6581..6979)
                     /locus_tag="VF036_02735"
     CDS             complement(6581..6979)
                     /locus_tag="VF036_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6264177.1"
                     /translation="MHPDPVRLGDLPLFGDLSDDDLRIIASWFEVEAVSEGRRIAPEG
                     ASGYLFYVIDEGTADVTHEGTAVASLGPGDHFGEMAMLGDGRRKADVVASSPMIIFAM
                     FGTAFREMEAGYPALAARIRATAEERLAEL"
     gene            complement(7080..7517)
                     /locus_tag="VF036_02740"
     CDS             complement(7080..7517)
                     /locus_tag="VF036_02740"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6264178.1"
                     /translation="MKTDISTSSSVAIDASVDDVWRAITTPELIKEWFFGVDTETDWK
                     QGSPIVHTGEWQGKPYVDKGEIVRIEPPHILVHTHWSDLSGTPDGAEHYQEVTWEVSE
                     RSDGSELTISERNLPSDEAKDASDQAWSIVLDNLKQLLEGDHR"
     gene            complement(7554..>7731)
                     /locus_tag="VF036_02745"
     CDS             complement(7554..>7731)
                     /locus_tag="VF036_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006899691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6264179.1"
                     /translation="VREVRSNSFEMEWPPRSGRTREFPEVDRAGWFGPEEARRKILRG
                     QLSFIDRLEELLAS"
BASE COUNT         1215 a         2601 c         2715 g         1200 t
ORIGIN      
        1 tcgtcctggt caccggcgga tcggtcgatc gcatgtcgaa cgcgccgctc gcgcacgagc
       61 tcgcgtccga cttcacggtc ctgaactacg atcgccgcgg ccgcggtccc agcggggaca
      121 cgccgccgta tgccatcgag cgcgagatcg aagacatcga ggcgatgctc gaggccgccg
      181 gcggctcggc gaacctctac ggctcgtcgt caggcgctgc cctggcattc caggcgacgg
      241 cggccgggct cccggtcacg aagctcgcgc tctgggagcc gccgtacatc atcgacgaga
      301 acgccaggcc gccgaaggat caggtcgagc agtacgagac gatggtggcg gagggacgca
      361 ggggagacgc ggccgagtac ttcatgacca aggtcgtggg catgccgccc gagttcgccg
      421 cgcaggcgcg gaacgagccc tggtggccga accaggaggc gctggcccac acgctcgcgt
      481 acgacgcgag gatcatgggc gactactcgg tgccgaccga tcaggccgcg tccgtgaagg
      541 tgcccaccct cgtggtcgct ggaggagccg acatgccgtt catgcgggag accgcgcaag
      601 cgctcgcgga tgcgctcccg gacggccagg tcaggctcct cgacggacag ggccacaacg
      661 tcgaccccac ggtcttggcg cccgcactca agggattctt cgagtgaggg cgctcacgtg
      721 aggttccgca ccacgttgga acaggccggc aagaccgcga ccggcttcga ggtcccgtcc
      781 accgtcgtgg agggtctcgg cgccgggaaa cggccggcgg tgcgcgtcac gatcaacggc
      841 ttcacctacc ggaacacgat cgcctcgatg ggcgggcggt tcatggtcgg ggtgagcgcc
      901 gagaaccgag cgggcgccaa ggtcgccggc ggcgacgtga tcgacgtcga catcgagctc
      961 gacaccgcgc cgcgcgtggt cacgatgccg aaggacttcg ccgccgccct caggaaggaa
     1021 ccgaaggcga aggcgacctt cgacgggctg tccaacagca acaagggatg gcacatctcc
     1081 tcgatcgagg gcgcgaagac cgacgagacg cggcagcgcc ggatcgcgaa gtcggtggct
     1141 gcgctgcgcg agggcaagcc gcgctgatcg aggatccccg gctacgctcc ctcgacatgg
     1201 tcgaggaaga gacgccggag ccggtcgatc gcgacgagga acgagcgcgg atcgcggacc
     1261 tcctcgccgt ccacgcgccg ccggactggg aggagcgctt cgagcgcgag cagctcctcg
     1321 acgcggtcat cgacacgacc cccttgcaga tgttcgtgat ggccgcggac atccgagagt
     1381 cgacgctgct gatgaaggaa gccgtcgagt tcgagcggtt cgcgaagatc atggacaagt
     1441 tcgtctccgc ggtccggagc gggatcggga cgccgggcgg gtggttcgac aagttcaccg
     1501 gcgacggctt cctcgcgtac tggatcgctc cctcgacgcc gaaggaggaa taccagcggc
     1561 ggttcgtgga ggccgccggc aatctcgcac acaccgcgca cgagctgatc gagctcttcc
     1621 atcggcgcgt gctcgaggac ttccggcgga actctcggaa cctgtccgac ggcgtcggcc
     1681 tcgcgatcgg cctcgacgcc ggaccgggct tcctcgtcga gattgccggg gagctcacgt
     1741 tggtaggacc gcccgtcgtg ggcgccgtgc ggatggtgaa cgcggcgtcg cgaccgcagg
     1801 agatcttcgc gaacgtctac ctgggtgagc acctccacga cgagcaggac ggcatctacg
     1861 aggcgctcgg catgacggcc acgcgcgaga gtcgtccgac caaggagtac ccgaagggtc
     1921 aggaggtcta cgccctgacg ttccgcgacg ccgagcagcc cgagggcggg tagggaggaa
     1981 cgtcagtcgg gcgtccatgg acgcagacgg ggccaccatc cgggaacggc gcgacgatag
     2041 gtctcgtacg cgtcgccgaa cgtcgccctc agggtcggtt cttcgtacac ccggacgaac
     2101 gccacgaaag cgaccgcgac gacgaagcca tatgccacca gctcgatacg accgaggatc
     2161 agcgcctgcc ccgcgatcgc ggcgaggacg gcaaggtaca tcggattcct tacgtaccga
     2221 tacagtccgc cgatcacgag ccgctgcgtc ggcgccaccg gggcgggtgt cccgagccct
     2281 tccgtcacga atcgagcgaa cgcggaaacc agcacgagga ccgcggcgac gagcaacgcc
     2341 gcgccgaacg cccgcagcgg ccagggagga ggatcagcga cctgccatcg cgtgatcatc
     2401 caagggagca gccctgcaac gatgcccggt gcaagcgcga agaagagtgt tgaccccacg
     2461 gcggctctcc accgtgccag cgaacccgaa gagggttcac ctccgggcgt gacggtagcg
     2521 gagatcggat cgccgtcttc caggccgggg gtcgatcgga gatggacctc gcacatgatc
     2581 tcgacgaggt cctcggggta gccgggttcg tcggcctgga acgcgatccc tcgatagcgc
     2641 ccggcgacga ggacatgcgc gaggtgatag ccggcctgtc cagtcgcggc ttcccaaccg
     2701 gggtcgatgt ccgatgctgc cacgtagcga gtcgtaaggc ttcggtcgag tgaggcgtcg
     2761 agccgcacgt tcaacgttcc cggcaccacg cgaagtccga agacctcctc gatccggtcg
     2821 agtacgagct cgtcggcgag cagctcggca ccgcgtccca agcccgcgac cacgacgcca
     2881 tgcagttcct gaccggtcac gatggaagga gcctacggac ccggtggttg gttcgagaac
     2941 aggatgggac ggccgtcgag ccgaaggagc gagtcgatgt tcacgcgtgc ggagttcggg
     3001 atcagcatca ctccggactg gtcgaagcag gaggagatcc tcgacctggc ccacgcggcc
     3061 gatgacggcg gcctcgacct gatcgggatc caggaccacc cgtaccagtg gcggttccac
     3121 gacacgtggt cgctgatcgc gttcctcgcg gggcagacct cgcggatcgg cttcttcccc
     3181 gacgtggcca acctcccgct ccgaccgccc gcgaccctgg ccaagtccgc cgcgtcgctc
     3241 gacgttctca cgggtggacg gatcgagctc ggtctcggcg cgggcagctt ctgggaggcg
     3301 atcgaagccg cgggcgggcc gagacgcaca ccggccgagg ccgtcgaggc gatcgaggaa
     3361 gcgatcgacg tgatgcggtt ggtctggagc ggcgagcgcg gcgtgcggtt cgaagggaag
     3421 cactatcggc tcgcgggctt ccacaccggt ccgaagcctg cgcacgacat cgggatctgg
     3481 gtcggcgccg gtcgacggcg gatcctggac ctgatcggga ggaaggccga cgggtgggtg
     3541 ccgtcctcgc cgtgggcgcc acccgaggcc ctccccggct acctgcgccg gatcgacgag
     3601 gccgccgcca ccgccggtcg cgatccggcc tcgatccggc gcgtctacaa cctgatgggc
     3661 gcgatccagc cggagagaag ggagcgcctc gtcgggcccg tctcgtactg ggtcgaggag
     3721 ctggccagct tggtggacat cggcatggac gcgttcatct tctggccggg aaccggcgac
     3781 ccgctcggcc aggtggaact cttcgcctcc gaggttgcgc cgtcggtccg tgcctcgacc
     3841 ggcggcccgt gaacctacac tgggagtcga tgcggttcgg acggttcgcc ttcgggtcga
     3901 tcgaggtcga tggcacgacc tacgagcacg atctcgtgat cgatcgcggg ggcatcagga
     3961 agcgcaagaa gggaccgtcg aaagccctcc gggcccgata tgggcacacg ccgctctcct
     4021 tgggcgagga catcccctgg gattgtgagc gtctcgtgat cggcagcgga gccgcgggat
     4081 cgttgcccgt cgtcgacgac gttgcggagg aagcagagcg gcgaggcgtc gagctcctgg
     4141 tactccccac gtcagaggcg atcgtggagc tggagcgagc accgggcggc tccaacgcga
     4201 tcctgcacgt gacctgttga ggagcgggtc ccgatacgtc aggcggccgt cagctcgacc
     4261 gggtggatcg ccatgctgcg aagggagccg tctcgccaga cgcgcgcctc catcgaccgg
     4321 ccgatcacct cgccgaccat cagccgctgc aggtcgcggg catcggtgat cggcgcgccg
     4381 tcgagctcca cgatcaggtc gccggatcgg atgcccgcac gcgctgcggg gctgccgtcc
     4441 accacctcca tgatctcgac gccttcctcc cggccgaccg ccgcagccgc tcggggcgga
     4501 agtgggcgcc cgccacccgc gatgccgatg taggcgcgct ggaacctgcc ggcggacatg
     4561 agggcgtgca tgatccgtcg cgtcgaatcg tcgatgggga cggcgagacc caacccgaac
     4621 cccgcgatcg cggtgttgat cccgacgacc cgcgcgcgcc cgtccgcgag cgcccctccc
     4681 gagttgccgg ggttgagggc ggcgtccgtc tggatcacgt tctccacgac tcggcctgct
     4741 tgcaccggga gggcccgccc gagcgcgctg acgacgccgg cggtgacgga gcccgcgaac
     4801 cccatcgggt tgccgatcgc cacgaccagt tgcccgacac gaagctctgc ggcctcaccg
     4861 agctccgccg atgcgaatcc gtctccctgg agccggagca ccgcgaggtc cgagagctgg
     4921 tcgcgtccca cgacgtcgaa cgccacctcg cgaccatcga cgaaggaggc cgatccgcgg
     4981 ttcgtgccgc cgaccacgtg cgcggaggtg agggcgaacc cgtcgggcgt gatgacgacg
     5041 gcggacccgg cgccgtccac gctcgcccct cgcgctcgct gctgcacccg gaggctcgcg
     5101 accgaaggga tcaggcgttc ggcgaccgac gtcacgacgc gcgagtacgc gtcgagcgcc
     5161 tcctcctcgt cccgtggcga tccgtcgtcc accccatcct tgtaccgcag atcgtgccga
     5221 acgatggcac gctgtggacc tggtcggcga aggcgaagga tccggtggga ccggttgtcc
     5281 cgaccgtgag accatgagcg ccgtgagcac cgagaggcat ccgcgactgc ttccggtggc
     5341 cgaggaggct cgcgtcacgc cgatcgagct gttcttcgac ctcgtgttcg tgttctcgct
     5401 cacccaggtc acggcgttga tgggcgacga tctgagctgg cacggggtcg ctcgcgggat
     5461 cctcgtgctc gcgctgctct ggtggtgctg ggtgggctac gcgtggctcg gcaacgtggt
     5521 gcaggccgag gaagggctcg ggaaggcgac gatgttcgcc tcgatggccg cgatgttcgt
     5581 gatggccctc tcggtgccgg aggcgttcga cgacctcgac ggcggtctcg acggaccggt
     5641 ggtgatcgcg ttcgcgtatc tcgcggtccg ggtgctccac ctcgccatct tctggatggt
     5701 ggccgggaca tcggaggatc cgggtctgcg agggcagctc atccgcttcg cgccttccct
     5761 ggtcggcagt acggcgatcc tgctggtggc gtcgcagctc tccggcacgg cgcagaccct
     5821 cacgtgggtc ggggcgctgc tcgtcgacta cgtgggcgtg atcctggggg gcgcgtcggg
     5881 ttggcggctc cggtcggcgt cgcacttcgc cgagcggcac gggttgatcg tgatcgtcgc
     5941 gctgggggaa tcgatcgtgg cgatcggtgt gggcgtgtcg gacctcccga tctcgtggcc
     6001 gatcatcgcg gcggcggttc tgggcctcgt ggtcgcggcg tgcctgtggt gggcgtactt
     6061 cgacgtcgtc tcgatcgtcg cggagcgcgt gctgcgtcgg ctcgagggcg aggagcggac
     6121 ccgcctcgcc cgcgacgcgt acagctatct gcatctcccg atggtcgccg ggatcgcgct
     6181 cgtcgcgcgc ggcatcgagg aggtcatgca ccaggtcggc catgcgaccg acggggaact
     6241 ctcccggacc ctggacatcg tgccgctggc gtcgctcttc ggcggcgcgg ccctcttcct
     6301 cttcggtcac gtcggattca aataccggac gtgggggtcg gtgacggtcc gccgcctggt
     6361 cgtcgcgctg ctgctctgtg cgttgatccc gctcgcagcc gagctcccct ccttgggcgc
     6421 gctcgggctg ctcgcagcca tcctcgtgac gatgatcgcg tcggaggcga tccggtacgc
     6481 cgaggagcgg gagcagatcc ggcacgaggc cgtcggtccc gaggtgcatc gcgagcatcg
     6541 cgaagccgat caggggatgg cggagcggtg aacccgatga tcagagctcg gcgaggcgct
     6601 cctcggccgt cgctctgatg cgagccgcca gagccgggta gccggcctcc atctcgcgga
     6661 aggcggtgcc gaacatcgcg aagatgatca tcggagagct cgccacgacg tccgccttgc
     6721 gacgcccgtc ccccagcatc gccatctcac cgaagtgatc gccgggtccg agcgacgcca
     6781 cggcggtgcc ctcgtgcgtc acgtccgccg tgccctcgtc gatgacgtag aacaggtacc
     6841 cggaggcgcc ctcgggcgcg atccgcctgc cttcgctcac cgcctcgacc tcgaaccacg
     6901 aagcgatgat ccgaaggtcg tcatcggaca ggtcgccgaa gagcgggagg tcgccgagtc
     6961 ggacgggatc ggggtgcacg atgcgcgttc gctcctgaga gaggttcggc gatcgtagtc
     7021 aacgaccacg cgtctgtccc tcggtgccac gatctgttcg gcagccgttc accgatcggc
     7081 tacctgtggt ccccctcgag cagctgcttc aggttgtcga ggacgatcga ccacgcctgg
     7141 tccgaggcat ccttcgcttc gtcggacggc aggttgcgct cggagatcgt gagctcggag
     7201 ccgtcgctcc gctccgagac ctcccacgtg acctcctggt agtgctcggc gccgtcgggc
     7261 gtgccggaga ggtcgctcca gtgggtgtgc acgaggatgt gcggcggctc gatcctgacg
     7321 atctcgccct tgtccacgta cggcttccct tgccattcgc cggtgtgaac gatcggagag
     7381 ccctgcttcc agtcggtctc cgtgtccacg ccgaagaacc actccttgat cagctccggg
     7441 gtcgtgatcg cgcgccagac gtcgtccacc gacgcgtcga tcgccacgga gctggacgtg
     7501 ctgatgtctg tcttcatatc gttcccttac tcagctccgg cgggcgggaa acatcagctc
     7561 gcgagcagct cctcgaggcg gtcgatgaag ctcagctgcc cccgaaggat cttgcgacgg
     7621 gcttcttcgg gaccgaacca tcccgctcgg tcgacctcgg ggaactcccg ggtccggccg
     7681 gagcggggcg gccactccat ctcgaaggag ttgctacgca cctcgcggac g
//
LOCUS       DASUVE010000132         7246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_630295, whole genome shotgun sequence.
ACCESSION   DASUVE010000132 DASUVE010000000
VERSION     DASUVE010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630295"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1136)
                     /locus_tag="VF036_02750"
     CDS             complement(174..1136)
                     /locus_tag="VF036_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX6264180.1"
                     /translation="MAACQLEALACVTGRLAGEEITWADEVERCLGVRPSRTDTSVLR
                     ETHGRLDAALPGNGSLRERYNAWDANNVVPREKLVLALERLQQVLGPRAHAIAPMPAE
                     ESVSYELVSNVPWIAYNRYEGRHHSRIEVNTDLPVSVVLLASLAAHDAYPGHHTDRVT
                     KDAHLFRDFGRLETSVAISAPESVVSEGIATNALDEALGPEPFAVVSDALADIDLRFD
                     PVEAHEVHEAELALYAPAVNAAFMLHEDGASTDDVEAYLREWALESNERAAQTAAFLT
                     DPSSRAYVSAYPDGRRLCRDFADRAPWNFTRLLTEQLTTTDLLP"
     gene            complement(1345..1821)
                     /locus_tag="VF036_02755"
     CDS             complement(1345..1821)
                     /locus_tag="VF036_02755"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6264181.1"
                     /translation="MSQSGPDRELIAEEDRLWTELHDLVDSMPADKVEEPGYFAEGWS
                     AKDLVAHIGSWTAEAGVVLDRLRSGTYRPEEIDIDAMNRQFYSSMRDVAFHDVRAQAV
                     TARNRMLRAWRSLPEGSAEADRWIRKSGPEHYAEHIPRLREWVGEIGRDDPGPHRG"
     gene            complement(1839..2897)
                     /locus_tag="VF036_02760"
     CDS             complement(1839..2897)
                     /locus_tag="VF036_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEX6264182.1"
                     /translation="MSVSEDSPRDGRSSRLVDSAIYVDGERVASPASLPETLETLRRQ
                     PEGMAWIGLYRPEEREVQSLASEFQLHPLAVEDAINAHQRPKLERHDDTLFVVLRPAR
                     YLDDIEEVEFGEVHVFVGPRFVLTVRHSEAPEFGSVRDRLEGEPDVLRRGPEAVLYAI
                     LDKVVDGYYPVVAGVHHDIDEIEVEVFSGDPKVSRRIYELSREVADFLRATRPLIDVI
                     TALRGGFAKYHVDERLQDHLRDVEDHLIQIIERVDEFRVALRDMLTVNATLVAQRQNE
                     EMRNLAEAANSQNEEVKKISAWAAILFAPTLIGTIYGMNFVHVPELDWVLGYPFALLL
                     MAVTCIGLYALFKRRRWL"
     gene            complement(3036..3320)
                     /locus_tag="VF036_02765"
     CDS             complement(3036..3320)
                     /locus_tag="VF036_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264183.1"
                     /translation="MNEWTEEATDARLGIDRPFDTYICECSDPRCTDPISLTRQEYES
                     VRAVPVRFAIALDHENPEIDRVLFENGRFATVEKFFGAGARIARSSDPRR"
     gene            complement(3364..3771)
                     /locus_tag="VF036_02770"
     CDS             complement(3364..3771)
                     /locus_tag="VF036_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6264184.1"
                     /translation="MGTHQSFTARVESRNGVTIIAMGGELDMASVPTLREHLMQVEGE
                     GPGVILADLRDLNFIDSSGLGALLEANKRSELNGHRFFTVGASPAARRLFQITGTEHL
                     LDDKDAVRFLDRFTRGQTGGQDRTRTARVDADA"
     gene            3943..4545
                     /locus_tag="VF036_02775"
     CDS             3943..4545
                     /locus_tag="VF036_02775"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX6264185.1"
                     /translation="MEKETIRRLRVLVTDGPGVRLDEVTRTVAGLGHEVVARESSLLE
                     VAHITAAEHPDVALVIVHEGNTKGLQMIDRIVHESACPVIAVIDVEDREFVNEAAKRG
                     IFAYIARGHDPQEMQSAIDIVLRRFAEYHALEGAFGRRAITERAKGILMERHGIDEEA
                     AFNMLRDQARRTHRKLVDLAEAVVTGQPVLPSQPRGKSEP"
     gene            4828..5253
                     /locus_tag="VF036_02780"
     CDS             4828..5253
                     /locus_tag="VF036_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010469480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6264186.1"
                     /translation="MDVREIRQVMTTPPDAVRKTDSLQEAARLMKTKDIGDVLVEDGQ
                     GLLAGILTDRDIAIRATAEGSDPKTTKVENAYTRDITALAPTDSVHDAVRMMRAQDVR
                     RLPVVEGGKAVGIVSLGDISVETAPGSLLADISAASPNR"
     gene            5398..6666
                     /locus_tag="VF036_02785"
     CDS             5398..6666
                     /locus_tag="VF036_02785"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HEX6264187.1"
                     /translation="MWLRKLAVSVGSSLWFVPVMCVVAGAAISFGTIALDRASDYQAI
                     PQVLVGRPTSANAILSSIAVSMVSLAALVLTITMVVVQLAMGQFSPRIVQRILRDRAS
                     QLAIGLFVATFVHAVLTIREVVDRGDGTGQVPGIAVATSYVLVLASIAALVIYVHHIG
                     QSLRVSALIELVGQDTRKLVDRRYPDAGPPPPVDAEAPRVVAARTSGVVTMIGTHALV
                     AEAARADCVLELVPALGAFVPAGGPLFIVHGGSEDLDEDRLFSALSLKLEPTLDEDVA
                     YGMRLLVDIAERSLSESPFQDPTTAVQAIDRLHDILRQLARRPFSDGRVRDEGGEIRL
                     LVKTMTWENYVHLAFDEIRMAGAGSPQVSRRLVAALMDLRRVALPERIEALDEQAVLL
                     RAATRDALQDEQEARSALDPDREGLGAAAP"
     gene            complement(6677..>7246)
                     /locus_tag="VF036_02790"
     CDS             complement(6677..>7246)
                     /locus_tag="VF036_02790"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX6264188.1"
                     /translation="NKTLAELFDGRSQLLAYNIMFGPDFEAGACPGCTSLADAFDGQL
                     IHLNKRDVTFLCFSRAPIERLSAYKKRMGWQFPYVSTHGTDFPFDFGLALTEGQAQQI
                     PELKEMIDEPPGWLQEWSEQVAAELKDGLRENPSYIAFARENGTVYHTYTVSAPDPFV
                     GPYFEMLLERAPKPQPDEPRSWRKDEYPF"
BASE COUNT         1194 a         2406 c         2424 g         1222 t
ORIGIN      
        1 acctgcgccg aagcgatgtg gcggttcagc gcatcggcga cgccgatcaa tccgtcgtcg
       61 tccagatcgg ggctcgcgaa ggacccgtac atgcgttcga gcgtatgggg aagtcggtca
      121 ccgcgcagtg acctatcgca cagctggcga gatctctttc cggaagggcc ggctcaggga
      181 aggaggtcgg tcgtggtgag ctgctcggtc aggagccgag tgaagttcca gggcgcccga
      241 tcggcgaagt cccggcacag gcgccgcccg tccgggtagg ccgacacgta cgcgcgggac
      301 gacggatcgg tgaggaacgc cgcggtctgc gccgctcgct cgttcgattc gagtgcccac
      361 tcgcgcaggt acgcctccac gtcgtccgtg gacgcgccgt cctcgtggag catgaacgct
      421 gcgttcaccg cgggcgcgta cagggcgagc tccgcctcat ggacctcatg cgcctcgacc
      481 ggatcgaacc ggaggtcgat gtctgcgagg gcgtccgaga cgaccgcgaa gggctcagga
      541 ccgagcgcct catccagcgc gttcgtcgcg atcccctcag agacgacgga ctcgggggcg
      601 gagatcgcga cgctcgtctc cagacggccg aaatctcgga agaggtgggc atccttcgtc
      661 acccggtcgg tgtgatgacc gggatatgcg tcgtgggcgg cgagggacgc gagcaacacg
      721 acggagaccg gcagatcggt attgacctcg atccggctgt ggtgccgtcc ctcgtaccgg
      781 ttgtaggcga tccacggcac gttcgagacg agctcgtagc tcacggattc ctcggcaggc
      841 atcggcgcga tcgcgtgcgc tcggggaccg aggacctgct gcaggcgctc gagcgcgaga
      901 acgagcttct cgcgtggaac cacgttgttc gcatcccagg cgttgtatcg ctcgcggagc
      961 gagccgttcc ccggaagggc tgcgtccagc cgcccgtggg tctctcgtag cacgctcgtg
     1021 tcggttcgag aaggacggac accgaggcat cgttcgacct cgtccgccca cgtgatctcc
     1081 tcaccggcaa gccggccggt gacgcatgcc agggcttcga gctgacacgc agccaccgcc
     1141 ggcggccttc cgagaggtcg gcgtcgccga gaccgtcgag gaggagctgc gcctcgtcgc
     1201 gggggccttt cggatccact gcctcttcgg cctcgaccgt ccgctccatc tccgaagggc
     1261 cgatgaacgc gtccacgaac ccctcgagat gccggcccac gcgcaggcag agccatacgt
     1321 atcgttccgg gatctccacg gcggtcaccc gcgatgagga ccgggatcat cccggccaat
     1381 ctcccccacc cactcccgga gtcgcgggat gtgctccgca tagtgctcgg gacccgactt
     1441 ccggatccag cgatcggctt cggccgatcc ctccgggagt gaacgccacg cccgcagcat
     1501 ccggttgcgg gccgtgacgg cctgcgcccg cacatcgtgg aacgcgacgt cgcgcatcga
     1561 gctgtagaac tgccggttca tcgcgtcgat atcgatctcc tcaggccgat acgtgccgga
     1621 tcgaagccgg tccaggacga cgcctgcctc ggcggtccag ctcccgatgt gcgcgacgag
     1681 atccttcgcg gaccaaccct ccgcgaagta gccgggttcc tcgaccttgt ccgcgggcat
     1741 cgagtcgacg agatcgtgca gctccgtcca caaccgatct tcttcggcga tcagctcccg
     1801 gtccggaccc gactgcgaca cgcgggtcag tatgacgcct agagccaacg cctccgcttg
     1861 aacaaggcgt agaggccgat gcacgtcacg gccatgagca ggagcgcgaa cgggtacccg
     1921 aggacccagt cgagctcggg cacatgaacg aagttcatcc cgtagatggt cccgatgagg
     1981 gtgggcgcga acaggatcgc ggcccaggcg gagatcttct tgacctcctc gttctgagaa
     2041 ttcgcagctt ccgccagatt gcgcatctcc tcgttctgcc gttgggcgac gagcgtggcg
     2101 ttcaccgtga gcatgtcccg gagagccacc cggaactcgt cgacgcgctc gatgatctgg
     2161 atcaagtggt cctcgacatc gcggagatgg tcctgaagtc gctcatcgac gtggtacttg
     2221 gcgaatcctc cacggagcgc cgtgatgacg tcgatcaacg ggcgagtggc gcgcaggaag
     2281 tccgccactt cacgggacag ctcgtagatg cggcgggaca ccttcggatc gccgctgaag
     2341 acctcgacct cgatctcgtc gatgtcgtga tgcacaccgg cgacgaccgg gtagtagccg
     2401 tcgacgacct tatcgaggat cgcgtagagc acggcctcgg gccctcgacg caggacgtcc
     2461 ggctccccct ccagtcggtc gcgcaccgaa ccgaactccg gcgcctcgct gtgacgaacg
     2521 gtcaggacga agcgcggacc gacgaacacg tggacctcgc cgaactcgac ctcctcgatg
     2581 tcgtccaggt agcgggcggg ccgaagcacc acgaacaggg tgtcgtcatg acgctcgagc
     2641 ttcggccgct gatgagcgtt gatcgcgtcc tcgacggcga ggggatggag ctggaactcc
     2701 gaggccagcg actggacctc ccgctcctcc ggacggtaca ggccgatcca tgccatcccc
     2761 tcgggctgcc ggcgcagcgt ctcgagcgtc tccggcagcg aggcgggaga ggcgacccgt
     2821 tcgccgtcca cgtagatcgc gctgtcgacg agccgcgaac tccgaccgtc ccgaggcgag
     2881 tcctcggata ccgacaggcc gtcgtcgcgg tcggcctcgt gacttcggcc ccccgacctc
     2941 cggcgaacga gggagcggat cggcgaccgg cggtgctcgg ttgggctcac gctcagagcg
     3001 taacccggct atcggcgcgt tcggagaggg cccggctagc ggcgaggatc gctgctgcgg
     3061 gcgatcctcg ctccggctcc gaagaacttc tcgaccgtgg cgaagcgccc gttctcgaac
     3121 agcacgcgat cgatctcagg gttctcgtgg tccagggcga tcgcgaaccg caccggcacg
     3181 gcccgcacgg actcgtactc ctggcgcgtc agactgatcg ggtccgtgca tcgggggtcc
     3241 gaacactcac agatgtaggt gtcgaagggt cggtcgatgc cgagccgggc atcggtcgcc
     3301 tcctccgtcc actcgttcat ctcccggaag atggtctgat tggtcgcttc gctctctcgc
     3361 caatcaagca tcggcatcga cgcgcgcggt tcgggttcga tcctgcccgc ccgtctgccc
     3421 acgcgtgaag cggtcgagga atctgaccgc gtccttgtcg tcgagcaggt gctcggtgcc
     3481 ggtgatctgg aagaggcgcc gagcggccgg ggacgccccc accgtgaaga accgatgccc
     3541 gttgagctcg gatcgcttgt tcgcctcgag gagggcgccg agaccggacg agtcgatgaa
     3601 gttcaggtcg cggagatcgg cgaggatgac ccccggaccc tcaccctcca cctgcatcag
     3661 gtgttccctc agggtcggaa cgctggccat gtcgagctca ccgcccatcg cgatgatcgt
     3721 gacgccgttc cgagattcga ctcgagcggt gaaggactgg tgggtcccca tggccctctc
     3781 cttccagcgc cgacgcatca ggcgccggtc gctacgcgag gaccatgggt cgggggcctc
     3841 tacctgccgg accggattct tgggtccgag aaccacagtc tactcccccg gcgaggacgc
     3901 agataccccc tgctagcatc gtggcagtct ttcggcaggc cgatggagaa ggagacgatc
     3961 cgccgtctgc gtgtcctggt cacggatggg ccgggagtac gcctcgatga agtcacccgc
     4021 acggtggccg ggttgggcca tgaggtcgtc gcccgcgagt cttccctgct cgaggtcgcg
     4081 cacatcaccg cggcagaaca ccctgacgtg gccctggtga tcgtccacga ggggaacacg
     4141 aagggcctgc agatgatcga ccggatcgtc cacgagtcag cgtgtccggt gatcgcggtg
     4201 atcgatgtcg aagacagaga gttcgtgaac gaggccgcca agcgggggat cttcgcctac
     4261 atcgcccgag gacatgaccc gcaggagatg cagagcgcca tcgacatcgt gctccggcgc
     4321 ttcgccgagt accacgctct cgaaggtgca ttcggccggc gggcgatcac ggagcgggcg
     4381 aaggggatcc tgatggagcg ccacggcatc gacgaggaag ccgccttcaa catgctccgc
     4441 gatcaggctc gtcggaccca tcgcaagctc gtcgacctgg cggaggccgt cgtgactggc
     4501 caaccggtgc tcccctccca accccgcggc aagagcgaac cctgatccag acgtccacca
     4561 ccaggaagag ctcaaccatc tggtgctcga tccccaactc atgggccgtt gttccgttcg
     4621 cgtgcgtggc caggacgtgc cctgggtaac gacctgataa ccgaggatcg gggaggaagc
     4681 gatatgggat tcatcagcac ggttgcgagc ttcggcatcg gatacgcggc agggatggtg
     4741 acgggccgcc aaggtatgga acggctgtct ggtcgtgtgc ggcagtcatc ttcgtcgagt
     4801 catggctctg gggacgccac ttccacgatg gacgttcggg agatccgcca ggtgatgacc
     4861 acgccacccg atgcggtccg gaagaccgac agtctgcagg aggctgctcg cttgatgaag
     4921 acgaaggaca tcggcgacgt gctggtcgag gatgggcagg gcctgcttgc cggcatcctc
     4981 accgacaggg acatcgccat ccgggccacg gcagaaggat cggacccgaa gacgaccaag
     5041 gtggagaacg cctacacgcg agacatcacc gccctcgccc cgacggattc ggtgcacgac
     5101 gcagttcgga tgatgcgagc ccaggacgtc cgccgcttgc cggtggtgga gggtggaaag
     5161 gctgtcggga tcgtctcgct gggcgacatc tcggtggaga ccgcgcccgg ctcactgctc
     5221 gccgatatca gcgcggcatc cccgaaccgc tgagttcgac gcggagctcg gccccgggtc
     5281 gccgggcctc tgatcgacgg cggggtcgac caccacatcc tcaagatcag ccacgtccgg
     5341 accggtcaag cactacccga tgcgtgcgac gtgggctcgt tgggtaacgc tcggcgtatg
     5401 tggttgagaa agctcgcggt cagcgtggga tcgagcctct ggttcgtgcc ggtcatgtgt
     5461 gtggtcgcgg gtgcggcgat ctccttcggc acgatcgccc tcgaccgcgc ctccgactat
     5521 caggcgatcc cgcaggtgct ggtcggccgg ccgacctcgg cgaacgcgat cttgagctcg
     5581 atcgcggtct cgatggtgag cctcgcggcg ctcgtgctga cgatcacgat ggtggtcgtc
     5641 cagctggcga tggggcagtt ctcgccccgg atcgtccagc ggatccttcg tgacagggcc
     5701 agccagctcg cgatcggtct cttcgtcgcg accttcgtgc acgccgtcct gacgatccgc
     5761 gaggtcgtgg accggggcga tggcacgggg caggttccgg gcatcgccgt ggcgacgtcg
     5821 tacgtcctcg tgctggcgag catcgccgcc ctggtgatct acgtgcacca catcggccag
     5881 tcgctgcgcg tatcagccct gatcgagctc gtcggtcagg acacacgcaa gctcgtcgac
     5941 cgccggtatc cggacgctgg cccgccgcct cccgtcgatg cggaggctcc gcgcgtcgtc
     6001 gctgcgcgaa catcgggcgt cgtgacgatg atcgggacgc atgcactggt cgccgaggcc
     6061 gcgcgggccg actgcgtgct ggagctcgtc ccggctctcg gcgcgttcgt ccccgcgggc
     6121 ggacccctct tcatcgttca cggcggttcc gaggatctgg atgaggaccg gctcttctcg
     6181 gcgctgagcc tgaaactcga gccgaccctc gacgaggacg tcgcgtacgg gatgcggctt
     6241 ctggtcgaca tcgcggagcg ctcgctctct gagtcgccgt tccaggaccc cacgacggcc
     6301 gtgcaggcga tcgaccgcct gcacgacatc ctccggcaac tcgcgcgtcg gccgttctcc
     6361 gacggccgag ttcgtgacga gggcggcgag atccgactgc tcgtgaagac gatgacctgg
     6421 gagaactacg ttcatctcgc cttcgacgag atccggatgg ccggcgcggg ctcgccccag
     6481 gtctcgcgac gcctggttgc cgcgttgatg gatctccgtc gcgtggcgct tccggagcgc
     6541 atcgaagcgc tcgacgagca ggccgtcctc ctccgtgccg ccacgcgtga tgccttgcag
     6601 gacgagcagg aggcccgctc cgcactcgat ccggaccgcg aaggtctagg ggctgccgcg
     6661 ccctagccgc cgcgattcag aacgggtact cgtccttgcg ccagctgcgc ggttcatccg
     6721 gctgcggctt cggcgcccgc tcgagcagca tctcgaagta gggaccgacg aacggatccg
     6781 gcgccgacac cgtgtaggtg tggtacacgg tcccgttctc acgcgcgaac gcgatgtagc
     6841 tcgggttctc gcgcagaccg tccttgagct ccgcggcaac ctgctccgac cattcctgga
     6901 gccagcccgg cggctcgtcg atcatctcct tcaactcggg gatctgctgt gcctgccctt
     6961 cggtcaatgc aagcccgaag tcgaacggga agtcggtccc gtgcgtcgag acgtagggga
     7021 actgccagcc catcctcttc ttgtaggcgg agaggcgctc gatcggcgcc cgggagaagc
     7081 agaggaacgt cacgtcgcgt ttgttcaggt ggatcagctg gccgtcgaac gcgtccgcga
     7141 ggctcgtgca cccggggcat gcccctgcct cgaaatcagg cccgaacatg atgttgtagg
     7201 cgaggagctg tgaccggcca tcgaacagct ctgcgagggt cttatt
//
LOCUS       DASUVE010000133         4386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1384351, whole genome shotgun sequence.
ACCESSION   DASUVE010000133 DASUVE010000000
VERSION     DASUVE010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384351"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..674
                     /locus_tag="VF036_02795"
     CDS             132..674
                     /locus_tag="VF036_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264189.1"
                     /translation="MTDEFQFGEPAKHPARLSGPRPPRPTSSFPFKLVGAVACLLVAA
                     AAAFVFLSGADEAGKEIADAPSETLAQVDRAYDAAAQGTVGRAVVVAQTLYAEQGTFT
                     TDLPTLSAFDPGLGFTSGPSTGPETVSYAVNGTEFAAAVRSESGACWWVKIDASSRTS
                     YGSGATCTGTAALAASSPSW"
     gene            734..1270
                     /locus_tag="VF036_02800"
     CDS             734..1270
                     /locus_tag="VF036_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264190.1"
                     /translation="MDDSFTYRTTLPQGPRVEPWLVKGAVVLVVLALGIGLFARWVVA
                     SERASFARGHQRVLPSTSVGQLRGSDPVGTDAEAEEATRIALAAARVALAGSGSFLAA
                     EPARLSALQPGFTFVDGPSTTSTIVSVAADRRVWAAAVLGASGTCFWARAHRDGAVET
                     GTSSTCTGASVLAIRSSR"
     gene            1368..1844
                     /locus_tag="VF036_02805"
     CDS             1368..1844
                     /locus_tag="VF036_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6264191.1"
                     /translation="MTHVSVTVNGAMREADVEPRTLLVYFLRETLGLTGTNVGCDTSS
                     CGSCTVLLDGESVKSCTLLAAQADGRELTTIEGMADGAELHPLQESFRRNHGLQCGYC
                     TPGVIMAAASYLKENPSPSEGDVRLALEGNLCRCTGYQNIVKSILDAAGSMAGVEA"
     gene            1854..4226
                     /locus_tag="VF036_02810"
     CDS             1854..4226
                     /locus_tag="VF036_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6264192.1"
                     /translation="MATTETFIGQPVLRKEDPELLTGHASYIDNWTMPGMLWMALARP
                     PYVHATIDGIDTTEAASMPGVAGVYTAADFELGPLPFVWPITEEIKVPVHYPLASDKV
                     RFNGDAVAVVLAETREQALDAAEAVAVTATELPAVIDIEEAAKDEVIVHDDLGTNVVV
                     HWNHGGGGDQSVFDSAPVIVQERYVQPRLIPNAIEPRGCLAYGVPPVDEWTLVTATQI
                     PHIAKVTLAGTTGIDEHKLRVIAPDVGGGFGSKLNVYAEEALALALARRLRRPVKWIE
                     GRRENYVATTHGRGVLHDCMLAGTEDGSILGMKFVELADMGAYYQLLTPGIPELGGWV
                     YMGPYDPKAYWFEFKGVVTNTTPTDAYRGAGRPEATYVIERLVDSFARRIGKDPADVR
                     RMNLHPPHEDAQTSIMGLNIDSANYEPLLDKALGLVEYDKLRAEQRDRRERGDRTQLG
                     IGLSTYIEMCGLAPSNILGALRYAAGGWDGATIECLPSGHVIVKTGTSPHGQSHETAW
                     AQIVADGLGVSPDEITVLHGDTHSTPLGMDTYGSRSVAVGGVALHFAVEKIKAKARTI
                     AAHELEVAEDDLEWGDGAFRVKGAPDTAKTIPELAVSAWHAHSLPEDVEPHLSATAVY
                     DPPNFTWPAGAHVCVVEVDTETGATEIVRYVAVDDCGTIINPMIVEGQIHGGVAQGVA
                     EALYEEAMYDESGNLTTSTMTQYLVPSATEIPAMTTDHSQETPSSTNPLGVKGIGEAG
                     TIAAPPAVVNAVIDAVSHLGVTAIEKPASPERVWRAIQEAKATGSQGGAS"
     gene            4223..>4386
                     /locus_tag="VF036_02815"
     CDS             4223..>4386
                     /locus_tag="VF036_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX6264193.1"
                     /translation="MIPAAFDYERVDTVDAAIAALQADEGAKLLAGGHSLLPQMKLRF
                     AMPTKLIDIGR"
BASE COUNT          682 a         1494 c         1581 g          629 t
ORIGIN      
        1 gatctcgctc atggagcgga tggtagccgg gcgacggacg ttcgatctcc cgagcctgcc
       61 cgctcaccgt gagacgtcga cgagatcgtt ctcgcttatg gggacatcgg agccggccga
      121 taccgggaga tatgaccgac gagttccagt tcggcgagcc tgcgaagcat cccgcgcgcc
      181 tgagcgggcc tcggccgccg cgcccgacga gctcgttccc gttcaagctg gtgggggccg
      241 tcgcgtgcct gctcgtggcc gccgcggctg cgttcgtgtt cctgagcggt gccgacgagg
      301 ccgggaagga gatcgccgac gcaccgtccg agaccctcgc gcaggtcgat cgggcgtatg
      361 acgcggcggc gcaggggact gtgggccgtg cggtggtggt ggcgcagacc ctgtacgccg
      421 agcaggggac gttcaccacc gacctcccga cgctctcggc gttcgacccg ggtctcgggt
      481 tcacctccgg cccgtcgacg ggccccgaga cggtctccta cgcggtcaac gggacggagt
      541 tcgcggccgc ggtccgatcg gaatcgggtg cctgctggtg ggtgaagatc gacgcgagca
      601 gcaggaccag ctatgggagc ggtgcgacct gcacggggac agccgccctg gctgcctcgt
      661 ccccgtcctg gtgatccctt cggcctgagc ccggagccct caggcctccg gccccggcgc
      721 cgatacgagg gggatggacg actcgttcac ctaccggacg acgctgccgc aagggccgcg
      781 cgtcgaaccg tggctggtca agggcgcggt cgtgctcgtc gtcctcgcgc tcgggatcgg
      841 gctgttcgca cggtgggtcg tggcgagcga gcgtgcgagc ttcgcccgtg gacaccagcg
      901 ggtgttgccg tcgacgtccg tcgggcagct ccgcggctcg gatcccgtcg ggaccgacgc
      961 cgaggctgag gaggcgaccc ggatcgcgct cgctgccgct cgcgtggccc tcgcgggttc
     1021 cgggagcttc ctcgccgccg agcccgcgag actgagcgcc ctgcagcccg gcttcacgtt
     1081 cgtggatggc ccgtcgacga cctcgacgat cgtctctgtc gcggcggatc ggcgcgtgtg
     1141 ggcggcggcc gtgctcgggg cgagcggcac ctgcttctgg gcccgcgcac accgcgacgg
     1201 cgccgtggag acgggcacgt cgtccacgtg caccggagct tcggtcctgg cgatccggtc
     1261 gtcccggtag ggctcccgat tcgaccgctc cgggcgccgg gtctacactc cgggctgctt
     1321 gcccgtcccc cacctcgagg ccccagaacg ggcagaagga ggcccccgtg acccacgtct
     1381 cggtcaccgt caacggggcg atgcgagagg ccgatgtcga gcctcgcacg ctcctcgtgt
     1441 acttcctccg cgagaccctc gggctcacgg gcacgaacgt cggatgcgac acgtcgagct
     1501 gcggctcgtg caccgtgctc ctcgacgggg agtcggtgaa gtcgtgcacg ctgctcgccg
     1561 cgcaggcgga cggccgtgag ctcacgacga tcgaggggat ggccgacggc gccgagctcc
     1621 acccgctgca ggagtcgttc cgccggaacc acggtctcca gtgcggctac tgcacgccgg
     1681 gcgtgatcat ggccgcggcc agctacctga aggagaaccc gagcccctcc gagggtgatg
     1741 tgcggctcgc gctggagggc aacctctgtc gctgcaccgg ctaccagaac atcgtgaagt
     1801 cgatcctcga cgcggccggc tcgatggccg gcgtcgaggc ctagggggcg acgatggcca
     1861 cgacggagac cttcatcggg cagccggtcc tccgcaagga ggatcccgag ctcttgacgg
     1921 gacatgccag ctacatcgac aactggacga tgccggggat gctgtggatg gccctggccc
     1981 gcccgccgta cgtgcacgcc acgatcgacg ggatcgacac gaccgaggcc gcgtcgatgc
     2041 cgggtgtcgc gggcgtgtac acggcggcgg acttcgagtt gggtcccttg ccgttcgtct
     2101 ggccgatcac cgaggagatc aaggtcccgg tccactaccc gctcgcgtcc gacaaggtcc
     2161 ggttcaacgg ggacgcggtc gccgtcgtgc tcgcggagac ccgcgagcag gcgctcgatg
     2221 ccgccgaggc cgtcgcggtc acggcgacgg agctgcccgc cgtgatcgac atcgaggagg
     2281 cggccaagga cgaggtgatc gtgcacgacg acctcgggac gaacgtcgtc gtgcactgga
     2341 atcacggggg cggcggcgac caatcggtct tcgactcggc gccggtgatc gtgcaggaac
     2401 ggtacgtcca gccgcggctg atcccgaacg cgatcgagcc gcgtggctgc ctcgcgtacg
     2461 gcgtgccgcc cgtggacgag tggacgctcg tcacggcgac ccagatcccg cacatcgcca
     2521 aggtcacgct cgccgggacc accgggatcg acgagcacaa gctgcgggtc atcgctccgg
     2581 acgtcggcgg cgggttcgga tcgaagctga acgtgtacgc ggaggaggcc ctcgcgctgg
     2641 ccctggcccg gaggctccgc cggccggtga agtggatcga gggccggcgc gagaactacg
     2701 tggccacgac ccacggtcgc ggcgtcctcc acgactgcat gctcgccggc accgaggacg
     2761 gctcgatcct cgggatgaag ttcgtcgagc tcgcggacat gggcgcgtac taccagctcc
     2821 tcacccccgg gatcccggag ctcggcggct gggtctacat gggcccgtac gatccgaagg
     2881 cgtactggtt cgagttcaaa ggggtcgtca cgaacacgac gcccaccgat gcgtaccggg
     2941 gcgccggccg gccggaggcg acttacgtga tcgagcgcct cgtcgattcc ttcgctcggc
     3001 ggatcggcaa ggatccggcg gatgtccgac ggatgaacct ccatccgccg cacgaggatg
     3061 cgcagacctc gatcatggga ttgaacatcg actcggcgaa ctacgagccg ctgctcgaca
     3121 aggcgctcgg tctcgtggag tacgacaagc tccgcgccga gcaacgcgac cggcgcgagc
     3181 gtggggaccg gacccagctc gggatcggcc tgtccacgta catcgagatg tgcgggctcg
     3241 cgccctcgaa catcctgggg gcgctgcgat acgcggccgg cggctgggac ggcgccacga
     3301 tcgaatgcct gccctccggt cacgtgatcg tcaagaccgg cacctcaccc cacggccaga
     3361 gccacgagac ggcgtgggcc cagatcgtgg ccgacgggct cggcgtctcc ccggacgaga
     3421 tcacggtgct ccacggggac acccattcga ccccgctcgg catggacacg tacggatcgc
     3481 gttcggtcgc ggtcggtggc gtcgccctgc acttcgcggt ggagaagatc aaggcgaagg
     3541 cccggacgat cgccgcgcac gagctggagg tggccgagga cgacctcgaa tggggcgacg
     3601 gcgcgttccg cgtgaagggt gcccccgata cggcgaagac gatcccggag ctcgccgtct
     3661 cggcctggca cgcgcactcg ctgcccgagg acgttgagcc gcacctcagc gcgacggccg
     3721 tctacgaccc gccgaacttc acctggccgg caggggcgca cgtctgcgtc gtcgaggtcg
     3781 acacggagac cggggcgacg gagatcgtga ggtacgtggc cgtcgacgac tgcggcacga
     3841 tcatcaaccc gatgatcgtc gaggggcaga tccacggcgg cgtcgcccaa ggcgtggccg
     3901 aggcccttta cgaggaggcg atgtacgacg agtcggggaa cctcacgacg tcgacgatga
     3961 cccaatacct ggtcccgagc gccacggaga tcccggcgat gacgacggat cactcacagg
     4021 agacgccgtc gagcacgaac ccgctcggcg tgaaggggat cggcgaggcc ggcacgatcg
     4081 cggcgccgcc cgccgtcgtg aacgcggtga tcgacgcggt ctcgcacctg ggcgtcaccg
     4141 cgatcgagaa gccggcatcg cccgaacgcg tctggcgagc gatccaggag gcgaaagcca
     4201 cgggatcgca aggaggggcg tcatgatccc ggcggcgttc gactacgagc gggtcgacac
     4261 ggtcgatgcc gcgatcgcgg ccctgcaggc cgacgagggc gcgaagctgc tggccggggg
     4321 acactcgctc ctgccgcaga tgaagctccg gttcgcgatg ccgacgaagc tgatcgacat
     4381 cggacg
//
LOCUS       DASUVE010000134         3305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_818391, whole genome shotgun sequence.
ACCESSION   DASUVE010000134 DASUVE010000000
VERSION     DASUVE010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818391"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VF036_02820"
     CDS             complement(<1..755)
                     /locus_tag="VF036_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6264194.1"
                     /translation="MSAKEQLDAAASVLDVERIRRDFPILKRTINGHPLVYLDSAATS
                     QKPRQVIDAVSDFYANHNANAHRGIYTLGEESTEAFEGARAKLAGFFGAPDPRTIVFT
                     RGTTESLNLVAQGWGRKFLKEGDEILLTDMEHHSNIVPWQLTAQVTGAVLRYLPLTDD
                     GQLDLSELGSLLNERTKILSVTGMSNALGTITPLGQLIEAAHAVGAVVAVDAAQLVPH
                     NGVSVSELDCDFLAFSGHKMLGPTASGGLYAKA"
     gene            complement(752..1540)
                     /gene="sufC"
                     /locus_tag="VF036_02825"
     CDS             complement(752..1540)
                     /gene="sufC"
                     /locus_tag="VF036_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014644162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX6264195.1"
                     /translation="MSENNGRAMLEIRDLHAEVEGKEILKGIDLTVTQGETHALMGPN
                     GSGKSTLSNIIMGRPGYVLTGGDVIFKGEKITGLTTDERAKRGLFLALQYPTEVPGVS
                     VVNFLRTAYQAVKGEQMSALAFRKHMKEQMDRLGIEDAMVQRYVNQGFSGGEKKRNEV
                     LQLAVLQPEIAILDETDSGLDIDSLKLVAGGVAELVGPELAVLLITHYQRILNYIAAD
                     RVHVMMQGRIVRSGGPELASELEEKGYEGIREELGIEDGGEEKR"
     gene            complement(1546..1863)
                     /locus_tag="VF036_02830"
     CDS             complement(1546..1863)
                     /locus_tag="VF036_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX6264196.1"
                     /translation="MSRVKVCEVAEAPAPGEAKRFPVDGRLVALVNLGDEGLRAIDAI
                     CSHAHYFLDEGDVDADFETIECPKHGSSFDLNSGKPRSLPATTPVDVFPITTEDDDIY
                     IEV"
     gene            complement(1860..3191)
                     /gene="sufD"
                     /locus_tag="VF036_02835"
     CDS             complement(1860..3191)
                     /gene="sufD"
                     /locus_tag="VF036_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX6264197.1"
                     /translation="MTAVSTTTFDERALESLPKAPSFVAALRADALERFASMPVPSQE
                     TEEWRYTDLSTFELDFMPFADGGGPEDLNRHGILASAGIAGERAGLQIQRNSTVISTQ
                     RSATLTDRGVWFGGLDEAAETHPELVEPFLHQLVPAGRTKFTALHAAFRTGGTFLHVP
                     RDTAIQLPIQTFTSLDADGAAVFPHTLVVVEAGSEVTFIDRYASPDLTRAFSDAITEI
                     HVGDGAHVRYVSIQEWGSGVTHLGIQRATVGRDATLRTLNIGFGASLARAEAETILAE
                     PGGFSEMLGIFFADEDQHFDHRSIQDHVAPNCTSDLLYKGALRDHSRAVYSGWVHVRP
                     DAQKTNAMQTSRNIVLSEHAKADAIPNLEIEANDVRCGHAASVGPVDEDAIFYLTSRG
                     IPRAEAERLIVSGFFQEVLDRVKIDEVREGAERAIQDELDRAALGQGEPNR"
BASE COUNT          483 a         1109 c         1118 g          595 t
ORIGIN      
        1 tcggccttcg cgtacagacc gccactggct gtggggccga gcatcttgtg gccgctgaac
       61 gcgaggaagt cgcagtcgag ctcgctgacg gagaccccgt tgtgcggcac gagctgcgcg
      121 gcatccaccg cgacgacggc acccaccgca tgggcggctt cgatgagctg gccgagcgga
      181 gtgatcgtcc ccagggcgtt ggacataccc gtcacggaca ggatcttggt ccgctcgttc
      241 agcagcgatc ccagctccga gaggtcgagc tgcccgtcgt cggtcagcgg gagatagcgg
      301 aggaccgcgc cggtcacctg cgccgtgagc tgccagggca cgatgttcga gtggtgctcc
      361 atgtcggtga ggaggatctc gtcgccctcc ttcaggaact tgcgtcccca accctgggcc
      421 accaggttca gcgattcggt ggtcccgcga gtgaacacga tcgtgcgagg atccggagcg
      481 ccgaagaacc cggcgagctt ggcgcgcgcg ccctcgaagg cctccgtcga ctcctcaccg
      541 agcgtgtaga tcccgcggtg agcgttcgcg ttgtgattcg cgtagaagtc ggagacggcg
      601 tcgatcacct gacgaggctt ctgggacgtg gcggccgagt ccaggtagac gagcgggtgg
      661 ccgttgatcg ttcgcttcag gatcgggaag tcgcgccgga tccgctcgac atccagcacg
      721 ctcgccgcgg cgtcgagctg ttccttggcg ctcatcgctt ctcctctccc ccgtcctcga
      781 tcccgagctc ctcgcggatg ccctcgtacc ccttctcctc gagctcgctc gcgagctccg
      841 ggccgccgga cctcacgata cgaccctgca tcatcacgtg gacgcgatcg gcggcgatgt
      901 agttgaggat ccgctggtag tgggtgatca gcaggaccgc gagctcgggg cccacgagct
      961 ccgccactcc cccggccacc agcttcagcg aatcgatgtc gaggccggag tcggtctcgt
     1021 ccaagatcgc gatctccggc tggaggaccg cgagctgcag gacctcgttc cgcttcttct
     1081 cgcctcccga gaacccctgg ttcacgtagc gctgcaccat cgcgtcctcg atgccgagac
     1141 ggtccatctg ctccttcatg tgcttgcgga acgcgagcgc gctcatctgc tcgcccttca
     1201 ccgcctggta ggcggttcgg aggaagttca cgacgctcac gccgggaacc tccgtcgggt
     1261 actgcagcgc gaggaacaga ccacgcttgg cccgctcgtc ggtcgtgagg ccggtgatct
     1321 tctcaccctt gaagataacg tccccgccgg tgagcacgta ccccggccgg cccatgatga
     1381 tgttcgacag cgtcgatttc cccgagccgt ttggccccat cagggcgtgg gtctccccct
     1441 gcgtgacggt caggtcgatg cccttcagga tctccttgcc ctcgacctcc gcgtggagat
     1501 cgcggatctc gagcatcgcg cggccgttgt tctcgctcat ggtcttcaca cctcgatgta
     1561 gatgtcgtcg tcctcggtcg tgatcgggaa cacgtccacc ggcgtcgtcg ccggcaggct
     1621 cctcggcttc ccgctgttca gatcgaacga agacccgtgc ttcggacact cgatcgtctc
     1681 gaagtccgcg tcgacgtccc cctcgtcgag gaagtagtgg gcgtggctgc agatcgcatc
     1741 gatcgcccgg agaccttcgt ccccgaggtt cacgagcgcc acgaggcgcc cgtcgaccgg
     1801 gaagcgcttc gcctcgccgg gagcgggcgc ctcggcgacc tcacagacct tgacccggct
     1861 catcggttgg gttctccctg tccgagggcc gcccggtcga gctcgtcctg gatcgcccgc
     1921 tcggctccct cgcggacctc gtcgatcttc acgcggtcga ggacctcctg gaagaacccc
     1981 gagacgatca gtcgctccgc ctcggcacga gggatcccgc ggctcgtcag gtagaagatc
     2041 gcgtcctcgt ccacggggcc gacgctcgcc gcgtgtccgc accggacgtc gttggcctcg
     2101 atctccaggt tggggatcgc gtcggccttg gcgtgctcgg acaggacgat gttgcgcgac
     2161 gtctgcatcg cgttcgtctt ctgggcgtcg ggccggacgt ggacccagcc gctgtagacc
     2221 gcgcgggagt ggtcgcgcag cgcgcccttg tagagcaggt cgctcgtgca gttgggcgcg
     2281 acgtggtcct ggatcgagcg gtggtcgaag tgctggtcct catcggcgaa gaagatcccg
     2341 agcatctcgg agaagccccc tggctcggcc aggatcgtct cggcctcggc gcgcgcgagg
     2401 gaggccccga acccgatgtt cagggtgcgc agggtcgcat cgcgtcccac cgtggcccgc
     2461 tggatcccga ggtgggtcac gccgctcccc cactcctgga tcgagacgta gcgcacgtgg
     2521 gcgccgtcgc cgacatggat ctcggtgatc gcgtcgctga aggccctcgt gagatcgggg
     2581 ctcgcgtagc ggtcgatgaa ggtgacctcc gagccggctt cgacgacgac gagcgtgtgc
     2641 gggaagactg ccgcgccgtc ggcgtcgagg gaggtgaacg tctggatcgg gagctggatc
     2701 gccgtgtcgc gggggacgtg caggaacgtt ccccccgtcc ggaaggccgc gtggagcgcc
     2761 gtgaacttcg tccggccggc cggaacgagc tgatggagga agggctcgac gagctcggga
     2821 tgggtctcgg cggcctcgtc caggccaccg aaccacacac cacggtcggt gagcgtggcg
     2881 ctccgctggg tggagatcac ggtggagttc cgttggatct ggaggccggc tcgctcgccg
     2941 gcgatccctg cggatgcgag gatcccgtgc cggttcaggt cttcgggacc gcctccgtcg
     3001 gcgaacggca tgaagtcgag ttcgaaggtc gacaggtccg tgtagcgcca ctcctctgtc
     3061 tcctgggacg ggaccggcat ggaagcgaag cgctccaggg catctgctcg cagcgcggcc
     3121 acgaacgatg gcgccttcgg aagggactcg agggcgcgct cgtcgaaggt ggtcgtactc
     3181 acggcggtca taacggaatc ctacccgaag ggtaggattc gccccgagtc actcccggat
     3241 ctctggtcgg tcatcgctcc tccccgtgat cggatcggcc tcgcccgtgg cccgggaggc
     3301 gttgg
//
LOCUS       DASUVE010000135         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1007759, whole genome shotgun sequence.
ACCESSION   DASUVE010000135 DASUVE010000000
VERSION     DASUVE010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007759"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..>2213)
                     /gene="cofH"
                     /locus_tag="VF036_02840"
     CDS             complement(281..>2213)
                     /gene="cofH"
                     /locus_tag="VF036_02840"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018552156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HEX6264198.1"
                     /translation="LMLESSSERLTEPGRPHHGSPDKVPAVRLRTIEDAGRLAIPFTT
                     GILVGIGEDERERAESLFAIRDLHRRYHHVQEVIVQNFRAKPRTAMHAAPEPDDETFL
                     AAVATARIVLGPHVSVQAPPNLADPAQRVRLLAAGINDWGGVSPLTPDHVNPERPWPE
                     VAALAATTAEGGATLRERLTIYPSFAARSDRWVAARMRAPVAALAGPDHLAVQGMRPA
                     PVAWQDPELGFKPRSIDLTFAKADDAGLRADAEDVYGSFETAEDPVTDAWRRASVDPV
                     RLDGDVRSALAAASAGRPITDDHALALFRADGAALDALCRVADDLRADVVGPDVTYVV
                     NRNINFTNVCYVGCRFCAFAQREVDPESYTLTLAEVADRAEEAWDRGATEVCMQGGIH
                     PDLPATFYLDLLDAVRERVPGMHIHAFSPMEIMNGATRMGVSYAEFLAEAKRHGLGTI
                     PGTAAEILDDDVRWLLTKGKLPADTWETIVRTAHGLGIRSSSTIMFGHVDAPPHWVAH
                     IRRIASIQRDTGGFTEFVPLPFIHRNAPIYLAGHARPGPSREDSLRMHAVARIMLHGQ
                     IDHIQVSWVKLGIDACQTILQAGANDFGGTLMEETISRMAGAEWGIEMTPDRFDDAIR
                     AIGRIPAVRTTTYDTVVRV"
BASE COUNT          320 a          777 c          777 g          339 t
ORIGIN      
        1 ggggaagaag acctccgggt cgtagtcacg acacctcgct cgttcctgcc aagggatgta
       61 gtggtgcacc tctcgctccc cccgaaatca catccgtgtg attctgaggg aaagatgccc
      121 tcggtgacaa gccctggccc aaggaaatct cctgcctcat ccctcgtccg tcggcgtcaa
      181 tcgtggaagg ctgcggggac ggttgcaagc gggttcggca ggaaaaacgg tggtcaacct
      241 ggttgacgga aatggtcaac cagatcgacc gtcgccgcga tcagacgcgc acgacggtgt
      301 cgtaggtggt cgttcgcacg gccgggatgc gtccgatcgc gcggatcgca tcgtcgaacc
      361 ggtcgggcgt catctcgatc ccccactccg cgcccgccat cctgctgatc gtctcttcca
      421 tcagcgtccc gccgaaatcg ttcgcaccgg cctgcaggat cgtctgacac gcgtcgatgc
      481 cgagcttcac ccacgacacc tggatgtggt cgatctgccc gtgcagcatg atccgcgcga
      541 ccgcgtgcat gcgcagcgag tcctcgcgcg acggtcccgg ccgcgcgtga ccggcgaggt
      601 agatcggcgc gttgcgatgg atgaacggca gcggcacgaa ctccgtgaac ccacccgtgt
      661 cgcgctggat cgacgcgatc cggcggatgt gcgcgaccca atgcggcggc gcgtcgacgt
      721 ggccgaacat gatcgtgctc gagctgcgga tcccgagccc gtgcgcggtg cggacgatcg
      781 tctcccacgt gtcggccggg agcttgccct tggtcagcag ccagcggacg tcgtcgtcga
      841 ggatctcggc ggccgtgccg gggatcgtgc cgagcccgtg gcgcttcgcc tcggccagga
      901 actcggcgta ggacacgccc atccgtgtcg cgccgttcat gatctccatc gggctgaagg
      961 cgtggatgtg catcccgggg acccgctccc ggaccgcgtc gagcaggtcg aggtagaacg
     1021 tcgccggcag gtcggggtgg atcccgccct gcatgcacac ctcggtcgcg ccccggtccc
     1081 acgcctcctc ggccctgtcg gcgacctcgg ccagcgtgag cgtgtaggac tccggatcga
     1141 cctctcgctg cgcgaacgcg cagaaccggc accccacgta gcagacgttc gtgaagttga
     1201 tgttccggtt gaccacgtag gtcacgtcgg gaccgacgac gtccgcccgc aggtcgtcgg
     1261 ccacccggca cagggcgtcc agggccgcac cgtcggcgcg gaacagcgcg agcgcgtgat
     1321 cgtcggtgat cggccggccc gccgacgcgg cggcgagcgc cgaccgcacg tcgccgtcca
     1381 gacggaccgg gtcgacggat gcgcgtcgcc aggcgtcggt gaccggatcc tcggcggtct
     1441 cgaacgagcc gtacacgtcc tcggcgtcgg cgcgcagccc ggcgtcgtcc gccttcgcga
     1501 acgtcaggtc gatcgacctc ggcttgaagc cgagctccgg atcctgccac gcgaccggcg
     1561 ccggccgcat cccctggacc gcgagatggt cgggacctgc cagggcagcg accggcgcgc
     1621 gcatccgcgc ggcgacccag cgatcggacc tggcggcgaa cgacggatag atcgtgaggc
     1681 gctcgcggag cgtggccccg ccctcggccg tcgtcgccgc cagggcggcg acctcgggcc
     1741 acggacgctc ggggttgacg tggtcggggg tgagcggcga gacgccgccc cagtcgttga
     1801 tgcccgcggc gaggagccgc acccgttgcg cggggtcggc gaggttgggc ggcgcctgga
     1861 cgctcacgtg cggcccgagc acgatcctcg cggtcgcgac cgccgcgagg aaggtctcgt
     1921 cgtcgggctc gggcgccgcg tgcatcgcgg tccgcggctt cgcccggaag ttctggacga
     1981 tcacctcctg gacgtggtgg tagcgacggt gcaggtccct gatggcgaac aggctctcgg
     2041 cgcgctcgcg ttcgtcctca ccgatcccca ccaggatccc ggtggtgaac gggatcgcga
     2101 gccgtcccgc gtcctcgatc gtgcgcaacc gcaccgcggg caccttgtcc ggcgacccat
     2161 ggtgcggccg gccgggctcg gtcagccgct cggacgagct ctcgagcatc agg
//
LOCUS       DASUVE010000136         2616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1573544, whole genome shotgun sequence.
ACCESSION   DASUVE010000136 DASUVE010000000
VERSION     DASUVE010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573544"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..2461
                     /locus_tag="VF036_02845"
     CDS             239..2461
                     /locus_tag="VF036_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6264199.1"
                     /translation="MTTEIEDSAVDPPRTTDGFFEPPAVGSADRTRELELRYRGIIDR
                     LPAVLYVDAVDRNQPMIDVSPSVHDLLGIPREEFLSRPLAWTDTIHPDDLAAVMAEND
                     RSAVTGDPFRMEFRVVHPDGKILWVRDDAVLIHDESGAPLHWLGLMLDVTELIEAQAE
                     LHEAKTKYGALVEQIPAIVYVDAADDEMSTTYVSPQIQAILGYTAQEYIDDPQLWERM
                     LHPDDRENALATYLRGRESGGPFVFEYRLIAHDGRTVWFRDSAIVLTDEQGRPEHIQG
                     VMLDITERKIAEERIAFLAYHDKLTGLPNRTMFDELLGLALARAKRHELGVVVVTVDL
                     DDFKLVNDSLGHEAGDAILMQFAQRLSDATRETDLVARPGGDEFLLLLADLDRASSGH
                     GGHESVSVSAESVALRIQEALRTPFIVGDTELYVTASLGISAFPDDASDAPTLLKNAE
                     TAMFRSKRSGPGGYVLHAKDDADSIVRLSLSTRLRRAVEQEQWMLHYQPLIDLRTAEM
                     NGVEALIRWPEPNGGLVPPGDFIPLAEEMGLIEAIGAWVIDEVCRQGRMWREQGLDLD
                     ISFNLSPRQLWQADIVDLIVDPILGSGMDPSRVTVEITESTAMTNPDRTLGILHELHA
                     RGLQLAIDDFGTGYSSLARLRYMPVDILKIDRSFVRELDRDEQNASMVSAMIALASNL
                     GMTPLAEGIETEAEWRALTERGCELGQGYFFSRPVPAGEILAIHRRAELHIVEGGVAG
                     "
BASE COUNT          432 a          911 c          887 g          386 t
ORIGIN      
        1 ggtcgtcgaa ggcgaggact gggacgccct tcacgggtcg gcgacggcag cgaaccagct
       61 caccccgtgg accgacgcgg gcgaccgata ctcgatcctg ttccgacccc tgctcccgga
      121 cgagcggggc tgctgagccc gttccgtccg gaactgagcg gccggtcaag tcccctcctc
      181 accgagccga tggttcagtc aggagcaggc cgccctcccg cggcctctgt gtggacccat
      241 gacgactgag atcgaggact ccgccgtgga cccaccgagg accaccgacg ggttcttcga
      301 gccgcccgcc gtggggagcg ccgatcgcac cagggagctc gagctccgct atcgggggat
      361 catcgaccgc ctgccggccg tgctctacgt cgacgcggtc gaccggaacc agccgatgat
      421 cgacgtgagc ccgagtgtcc acgacctgct ggggatcccc cgcgaggagt tcctcagcag
      481 gccgctcgcg tggaccgaca cgatccaccc cgacgacctc gccgcggtca tggcggagaa
      541 cgaccgctcc gccgtcaccg gagacccgtt ccgtatggag ttccgcgtcg tacaccccga
      601 cgggaagatc ctgtgggtcc gcgacgacgc ggtcctgatc cacgacgagt ccggagcgcc
      661 gctccactgg ctcgggctga tgctcgacgt cacggagctg atcgaggcgc aagccgagct
      721 ccacgaggcc aagaccaagt acggcgccct ggtggagcag atcccggcga tcgtctacgt
      781 cgacgcggcc gacgacgaga tgtcgacgac gtacgtgagc ccccagatcc aggcgatcct
      841 cgggtacacc gcgcaggagt acatcgacga cccccagctg tgggagcgca tgctgcatcc
      901 cgacgatcgc gagaacgcgt tggccacgta cctgcgcggt cgggagtccg gaggcccatt
      961 cgtcttcgaa taccgactca tcgcgcacga cggcaggacc gtctggttcc gcgacagcgc
     1021 gatcgtgctc accgacgagc agggccgccc cgagcacatc cagggcgtga tgctcgacat
     1081 caccgagcgc aagatcgcgg aggagcggat cgcgttcctc gcgtaccacg acaagctcac
     1141 gggactcccg aaccgcacga tgttcgacga gctcctcggg ctggccctcg ccagggcgaa
     1201 gcgccacgag ctcggcgtcg tcgtcgtgac ggtcgacctc gacgacttca agctcgtgaa
     1261 cgactccctc gggcacgagg ccggagacgc gatcctgatg cagttcgcgc agcggctcag
     1321 cgacgcgaca cgcgagacgg acctcgtggc gcgaccgggt ggggacgagt tcctgctcct
     1381 gctcgccgac ctcgatcgtg catcctccgg ccacggcggc cacgagagcg tctcggtctc
     1441 cgcggagtcg gtggcgctgc ggatccagga ggcgctccgc acccctttca tcgtaggcga
     1501 taccgagctc tacgtgaccg cgagcctggg gatcagcgcg ttcccggacg acgcgagcga
     1561 cgcgccgacg ctgctcaaga acgcggagac ggcgatgttc cgatcgaagc gatccgggcc
     1621 gggcgggtac gtcctgcacg cgaaggacga cgccgactcg atcgtgcggc tgtcgctgtc
     1681 cacgcggctg cggcgggccg tggagcagga gcagtggatg ctgcactacc aaccgctgat
     1741 cgacctgcgc accgcggaga tgaacggcgt cgaggcgttg atccgctggc ccgaaccgaa
     1801 cgggggcctc gtgccgcccg gcgacttcat cccgctcgcg gaggagatgg gcctgatcga
     1861 ggcgatcggc gcctgggtga tcgacgaggt ctgccggcag ggtcgcatgt ggcgagagca
     1921 gggactggat ctcgacatct cgttcaacct gtcgccccgg cagctctggc aggccgacat
     1981 cgtggacctg atcgtcgatc cgatcctcgg atcggggatg gacccgagcc gtgtgacggt
     2041 cgagatcacc gagtccacgg ccatgacgaa tcccgaccgc acgctcggca tcctccacga
     2101 gctccacgcc cgggggctcc agctcgcgat cgacgacttc gggaccgggt actcctcgct
     2161 cgcccggctc cgctacatgc cggtcgacat cctgaagatc gaccgttcgt tcgtgcgtga
     2221 gctcgatcgc gacgagcaga acgcgagcat ggtctcggcc atgatcgcct tggcctccaa
     2281 cctcgggatg acgcctctcg ccgaggggat cgagacggag gcggagtggc gcgcgctcac
     2341 ggagcgcggc tgcgagctgg ggcagggcta cttcttctcc cggccggtgc cggccgggga
     2401 gatcctcgcg atccaccggc gcgccgagct ccacatcgtg gagggcggcg tggcggggtg
     2461 atcgtggacg cgtcgccggc gctcacgatc ggcgagacca tcttctcggc gaggtcgggc
     2521 tgccacagct gccgggggga gaggttgaat cccagttcga gctcgagccc ctcggcccgc
     2581 cacatcgcgt cctgacggca gatctcctcg accacc
//
LOCUS       DASUVE010000137         4522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_632299, whole genome shotgun sequence.
ACCESSION   DASUVE010000137 DASUVE010000000
VERSION     DASUVE010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632299"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..957)
                     /locus_tag="VF036_02850"
     CDS             complement(148..957)
                     /locus_tag="VF036_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX6264200.1"
                     /translation="MTFSIVAADPDAGDWGVAVASKFVSVGAVVPWARAGVGAVATQS
                     WANTSFGPEGLALMADGASAEDALGTLIDGDEGHADRQVGVVDATGRGFAAQGNILVG
                     EQVVRAMSGAFAETDGDLCDRLLAALLAGDAAGGDRRGRQSAALLVVREGGGYEGRND
                     RYIDLRVDDHLDAPRELARLFDVYDATVLIRNDPLLPAAPDLVADLQRRLAALGRYAG
                     EATGVYDDATRAALAGWAGEYNLEGRLRDDDQISNQLVEEIRDVTPELPPP"
     gene            complement(962..1969)
                     /locus_tag="VF036_02855"
     CDS             complement(962..1969)
                     /locus_tag="VF036_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX6264201.1"
                     /translation="MGRDLAIDLGSANTLVYRQGDGIVFDEPTVVALHGGTGDVVAMG
                     EEAWQMLGGDSGNVLAVRPLREGTMTEFDVTQRMLAVVLRRVGVTRFPKPRVLVCVPP
                     LSTNVERRAIERAIRYAGGRQVVPVEEPLAAAVGANLPVHEPLGNLIVDIGGGRTEMA
                     VISMGGVVSGRSASVGGFDLDAAIQEHIRITYGVSIGEKSAEELKVAIGSAFPSPSGR
                     AAKVIGRELASGNTVEVKVDEDEIRQAMAGAVKTIVDEARMTLAEAPPELTHDVLETG
                     MFLTGGGSLLRGLDMLLAQECEVPVHVVERPLETVVSGAGRMLEHLDDYRASFQLLRR
                     G"
     gene            complement(1970..2395)
                     /locus_tag="VF036_02860"
     CDS             complement(1970..2395)
                     /locus_tag="VF036_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX6264202.1"
                     /translation="MSELVLTRVSADPLSVEEALAFIADPGAGGTCVFIGTVRDRSDA
                     GEVTGLHYEAWDDLAAGRLGEIAHDMLDRWPLRRVAILHRTGDLAIGEASVVVACSAP
                     HRAEAFEACRHGIERLKEDVPIWKKEGLVSGDARWVMGS"
     gene            2447..3613
                     /locus_tag="VF036_02865"
     CDS             2447..3613
                     /locus_tag="VF036_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX6264203.1"
                     /translation="MSGFDFGSGDPEAFRDAPLFRELQRVMSASSGPVNWELARQVGV
                     ANAVEAGPDPEPTEGDHRMLEEAVRVAELQVGRFTGLAAPADVAQVLAVRRAGWVNAN
                     AENLKALLEPAAGKLTDAIQRSAGDQLPPEMAQFSAFLRQLSPLLLGTQVGQVLGTLA
                     QRVLGQYDIAVPRSGPGQLLFVVPNLAAFERDWSLDPREFRTFVALHEVTHRFEFAQP
                     WARDRFQALLDDYLSTLKLDVEGMQARLSSVDPADPEALRELAGSDEGLFWADLDDEQ
                     RLKLGRIQAFMAAAEGYGDHVMHALGAELLGSSARIEEAMKRYREGEPGDPVFERLLG
                     VDMKREQYRKGRAFCEVVVEQSDEAILARMWESADAMPSLPELDEPRLWLARTV"
     gene            3657..4451
                     /locus_tag="VF036_02870"
     CDS             3657..4451
                     /locus_tag="VF036_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264204.1"
                     /translation="MSIGELISAAVVDERVWDAYQETAPDGVDLVGQPGPALPFVVFR
                     AWKVPSGFVSEEVRLVGPSGRTLYRWGPEVRRMKGAMDLTTEVDRIADAVVDETGTYV
                     ASFILEGEVLGEIEFPVYVQAAAQKLPKETEEGLRKSDVIWVGTRVNGHKRMAPVWFA
                     YKEGKVYVLSSRDADLQEQQVPGVPEAQELVVVTRRKGRDTSLEEFTAAQRLLEGPEW
                     ENAAKMLVDRRRSRVGPPQESLARWRGKVDIVELTPNVGTLETTDS"
BASE COUNT          679 a         1597 c         1578 g          668 t
ORIGIN      
        1 cgcgcgcgat cgactcacgt ggcacgatcg gatcatcggg accctcggcc tcgtggtcgc
       61 ggtgaccgtc gcggcggcca cgctcgcgtt cgtcgtctac ttcgtcgcct cgacggccct
      121 atcgttgctg gagaaggccg cgggccccta ggggggaggc agctccgggg tcacgtcccg
      181 gatctcctcg accagctggt tcgagatctg gtcgtcgtcg cggagccggc cctccaggtt
      241 gtactcgccg gcccaccccg cgagagcggc gcgcgtcgcg tcgtcgtaga ccccggtcgc
      301 ctcacccgcg tacctcccca gcgcggccag gcgccgctgc agatccgcga cgaggtcggg
      361 ggcggccggc aggagggggt cgttgcggat caggacggtc gcgtcgtaga cgtcgaagag
      421 ccgagcgagc tcgcggggcg cgtcgagatg gtcgtccacc cggaggtcga tgtagcgatc
      481 gttgcggcct tcgtagccac ctccctcgcg gaccacgagg agcgcggcgg actgtcggcc
      541 gcgcctgtcc ccgccggccg catcacccgc gagcagcgcc gcgaggagcc ggtcgcacag
      601 gtcgccgtcc gtctccgcga acgccccaga catcgctctc accacctgct cgccgaccag
      661 gatgttcccc tgcgccgcga acccgcggcc ggtcgcatcc accacaccga cctgccgatc
      721 cgcatggccc tcgtcgccgt cgatgagcgt gcccagggca tcctccgccg atgcgccgtc
      781 cgccatcagc gccagaccct ccggtccgaa cgatgtgttc gcccacgact gcgtcgcgac
      841 cgcgcccacg ccggccctcg cccacgggac gaccgcgccc accgagacga acttcgaggc
      901 caccgcaacg ccccagtccc cggcatccgg atccgcggcg acgatcgaga acgtcatcgg
      961 ctcatcctcg gcggagcagc tggaaggagg cccggtagtc gtcgagatgc tccagcatcc
     1021 gcccggcccc gctgacgacg gtctcgagcg ggcgctcgac cacatggacc ggcacctcgc
     1081 attcctgggc gaggagcatg tccaatccgc ggaggagact gccgccgccg gtgaggaaca
     1141 tccccgtctc caagacgtcg tgggtgagct ccggcggcgc ctccgcgagg gtcatccgcg
     1201 cctcgtcgac gatcgtcttc accgcccctg ccatcgcctg ccggatctcg tcctcgtcca
     1261 ccttgacctc cacggtgttc ccggacgcga gctcgcgtcc gatcaccttc gccgcccgac
     1321 ccgagggcga ggggaacgcc gacccgatcg ccaccttcag ctcctccgcg gacttctccc
     1381 cgatcgagac accgtaggtg atccggatgt gctcctggat cgccgcgtcg aggtcgaagc
     1441 cgccgacgct cgccgagcgc ccggacacga cgccccccat cgagatgacg gccatctcgg
     1501 tccgtccgcc gccgatgtcg acgatcaggt tcccgagggg ctcatgcacc gggaggttgg
     1561 cgcccaccgc cgccgccaac ggctcctcga cggggaccac ctgtcgcccg ccggcgtagc
     1621 ggatcgcccg ctcgatcgcg cgacgctcga cgttcgtcga cagcggtggg acgcagacga
     1681 ggacgcgcgg tttcgggaac cgcgtgacgc cgacacgccg gaggacgacc gccagcatgc
     1741 gttgtgtcac atcgaattcg gtcatcgtgc cttcgcggag gggacggacc gccaacacgt
     1801 tgcccgagtc gccgccgagc atctgccagg cttcctcccc catcgcgacc acgtccccgg
     1861 tcccgccgtg caatgccacg acggtgggtt cgtcgaacac gatgccgtct ccctgccgat
     1921 acacgagggt gttggcactg ccgagatcga tcgcgaggtc gcggcccaac tagctcccca
     1981 tgacccaccg cgcgtcgccg gagacgagcc cttccttctt ccagatgggg acgtcctcct
     2041 tcaggcgctc gatgccgtgc cgacacgcct cgaaggcctc cgcgcgatgc ggggcggagc
     2101 acgcgacgac gaccgaggcc tccccgatgg ccaggtcccc ggtgcgatgc aggatcgcga
     2161 cccgcctgag cggccatcga tccagcatgt cgtgtgcgat ctctccgaga cgcccggccg
     2221 cgagatcgtc ccacgcctcg tagtgcagtc cggtcacctc gcccgcgtcg gaccggtctc
     2281 ggaccgtgcc gatgaagacg cacgtaccgc cggcacccgg atcggcgatg aacgccaggg
     2341 cttcctcgac ggagaggggg tcggcggaga cgcgggtcag cacaagctcg gacacggcgg
     2401 ccatcgtatc cggagggcca cgtacgatgg ccagggtgat gaacagatga gcggcttcga
     2461 cttcggctcc ggcgaccccg aggcgttccg ggacgcaccc ctcttccgag agctccaacg
     2521 cgtcatgtcc gcgtcgagcg ggccggtcaa ctgggagctc gcgcgccagg tgggtgtcgc
     2581 gaacgccgtg gaggctggac ccgacccgga acccaccgag ggcgaccacc ggatgctcga
     2641 ggaggccgtg cgcgtcgcgg agctgcaggt cggccggttc accgggttgg cggcgccggc
     2701 ggacgtcgcc caggtgctgg cggttcgacg agcgggttgg gtcaacgcga acgccgagaa
     2761 tctgaaggcc ctgctcgagc cggccgcggg gaagctgacc gacgcgatcc aacggagcgc
     2821 cggggaccag ctccccccgg agatggcgca gttctcggcg ttcctccgcc agctctcacc
     2881 gctcctgctc ggaacgcagg tgggccaggt gctggggacg ctcgcgcagc gggtcctcgg
     2941 ccaatacgac atcgccgtgc cgcggtccgg ccccggtcaa ctcttgttcg tcgtgccgaa
     3001 cctcgcggcg ttcgagcggg attggtcgct cgatcctcgc gagttccgca cgttcgtcgc
     3061 gctgcacgag gtgacccatc ggttcgagtt cgcgcagccg tgggcgcgcg accggttcca
     3121 ggcgctcctc gacgattacc tctcgacact gaaactggac gtcgagggca tgcaggcgcg
     3181 actcagctcg gtcgatccgg ccgaccccga ggccctccgc gagctcgcgg ggtcggacga
     3241 gggcttgttc tgggcggacc tggacgacga acagcggctg aagctgggcc ggatccaggc
     3301 cttcatggcg gcggctgagg ggtacggcga ccacgtgatg cacgccctcg gcgccgagct
     3361 gttggggagc tcggcgcgga tcgaggaagc catgaagcgg taccgcgagg gcgagcccgg
     3421 cgatccggtg ttcgagcggc tgctcggcgt ggacatgaag cgtgagcagt accggaaggg
     3481 acgagccttc tgcgaggtcg tggtcgagca gtcagacgag gccatcctcg cgcggatgtg
     3541 ggagtcggcc gacgcgatgc cgtcgctgcc ggaattggac gagccccggc tctggctcgc
     3601 ccgtaccgtc tagcgactcc tggtcgcccg gggccggtcg gctatcatcg ccggcgatgt
     3661 ccatcggaga gctgatctcg gcggcggtcg tcgacgagcg ggtctgggac gcctatcagg
     3721 agacggcccc ggacggcgtg gacctcgtcg gtcagccggg cccggcactc ccgttcgtcg
     3781 tgttccgggc ctggaaggtc ccctccggct tcgtctccga ggaggtcagg ctcgtcgggc
     3841 cgtcgggccg gacgctctac cggtgggggc cggaggttcg ccggatgaag ggggcgatgg
     3901 acctcaccac cgaggtcgac cggatcgccg atgcggtggt ggacgagacc ggaacctacg
     3961 tggcgtcgtt catcctcgaa ggcgaggtcc tcggggagat cgagttcccg gtctacgtgc
     4021 aggcggcggc gcagaagctc cccaaggaga ccgaggaggg actccggaag tccgacgtga
     4081 tctgggtcgg cactcgggtg aacggccaca agcggatggc gccggtgtgg ttcgcgtaca
     4141 aggaggggaa ggtgtacgtg ctgtcgagcc gagacgccga cctccaggag caacaggtac
     4201 ccggcgtccc ggaggcgcag gagctggtgg tggtgacccg acggaaagga cgtgacacgt
     4261 cgctcgagga gttcaccgcc gcgcagcggc tgctggaggg acccgagtgg gagaacgcgg
     4321 cgaagatgct cgtggatcgc cgccggagcc gggtcggtcc accccaggag tcactcgccc
     4381 gctggcgcgg gaaggtcgac atcgtcgagc tcacgccgaa cgtcggcacg ctcgagacca
     4441 ccgattccta gcggttcatc gctcggtcca tccccccttc gctcgggtgg gctcgtcgat
     4501 gagaggtgag gagatgttca ga
//
LOCUS       DASUVE010000138         4124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_256050, whole genome shotgun sequence.
ACCESSION   DASUVE010000138 DASUVE010000000
VERSION     DASUVE010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256050"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VF036_02875"
     CDS             <1..643
                     /locus_tag="VF036_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264205.1"
                     /translation="EPFGPVDPGAQQILLDGSGVAYYEPAGEGFGRRLAIHADGADVF
                     LETNLPRDRLLEIASSLRLVGRPLPPERRDRGPAGIGVERIDVTEGLTAAGLPASLAR
                     SLPRGYVVASAERSTVGSTPEGVTLHLRQIDTDAAGEPLTLHVEHDTGMPRPWSSEQG
                     SITFAGLEGRWTPSRSQLEWVDRGVYHSLQGAMDLRSLIEIAGAIAAVRTGAG"
     gene            640..1851
                     /locus_tag="VF036_02880"
     CDS             640..1851
                     /locus_tag="VF036_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX6264206.1"
                     /translation="MRRSLRYGAGGLTGVALGIVLTLAIQAASLPSTPEAPTGPSLLL
                     TRPAPPETFLAWVPRGLPDTFAARVARQPEIGRLTVVAEDNVWLARSWSAAGELVDRP
                     VRPYRIPLDVAAVDPETFAPFLPPADQATLAAIEDGEGILGSTSASVRRLGPGGLLEL
                     GRGKLIRIAAVWPDELVGASELLVSKETGRRIGVTRERYVLVRPAAGGSITSGSLRRA
                     IQPLLPRDLGVFRRVQVRSPGETPYFRAGDAVLPPVLLKAFFGEFAARPAPGQPGSLE
                     VDPEWIRTHLVTTGIPLLGRVTCHTGIVPQLRGAMRELRDAGAAGVIRSFHGCFVPRF
                     ISRDPTALLSHHSWGVAFDVNLVGNYYGVKPHQPPRLRRTLARWGFIWGGTFVVPDGN
                     HFEYRRLPKRG"
     gene            complement(1858..3492)
                     /locus_tag="VF036_02885"
     CDS             complement(1858..3492)
                     /locus_tag="VF036_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6264207.1"
                     /translation="MTVSERIVAQLAAAGVRRAYTVPGESFLPLLDACDRHPDVALIS
                     TRHESGAAFMAEADAKVTGRPALAMATRGVGAANLSIGVHTASQDSTPMVVILGQVES
                     AFLGREAFQEVDLPSFYREITKWAVTAPSSERLPELVGRAIHVATSGRPGPVMIAVPA
                     DLFDGDAGPEQSHLPGVVDRDRPTPSERTSERIADVLRDAVAPVMIVGGGGRDARDAL
                     VTTAERFGLGVYASFRRQDVFPNDHPNYCGPLVLSTPPETLRALEDADVVFVVGCRLS
                     EITTQGYRLPSADQRVIQIDVEPNSVGAVVPVELGLVADAREALTAILDHAPDTPADR
                     DWTAAHGAFLRASTLPDATDGPLHPAAVIAALAQVFPPSVVLVNDAGNFSIFAHRYWR
                     FSHPRTQIGPTSGAMGYGVPGGVGAKLADPSSEVVVLAGDGGFLMTGQELETAVRHDV
                     PFTTVVFRNGLYGTIALHQARAYGRTVGVDIGDVDIAAVARGYGAEAWSVSRAPELIA
                     ALTAARACGRPAVVDCRVDPDVLSPTDRLSALVGNG"
     gene            complement(3574..>4124)
                     /locus_tag="VF036_02890"
     CDS             complement(3574..>4124)
                     /locus_tag="VF036_02890"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264208.1"
                     /translation="ANRGPHPSVDLPTTTKEARMVSGKIKVLAVAALVPLVMGACSED
                     VEPTDGTSPPTESSEPSEEPAEATVAVEDSDFGQIVVDAAEGRTLYVFLADTGSDSTC
                     YDECEDNWPPLTVEGDPAAGEGIDASLLGTTEREDGSVQVTLDGHPLYYFAADETPDD
                     INGQAVSDVWYVVSPEGEAIKE"
BASE COUNT          560 a         1501 c         1455 g          608 t
ORIGIN      
        1 cgagccgttc ggacccgtgg acccgggcgc gcagcagatc ctcctcgacg gcagcggggt
       61 cgcctactac gagcccgcgg gagaggggtt cgggcgtcgg ctcgcgatcc atgccgacgg
      121 tgccgacgtc ttcctcgaga cgaacctccc tcgggaccgg ttgctcgaga tcgcgtcgtc
      181 cctccggctc gtcggccgtc cgcttcctcc cgagcggcgc gatcgagggc cggccggcat
      241 cggcgtcgaa cggatcgacg tgaccgaggg gctcaccgcc gcgggcctgc ccgcatcctt
      301 ggcgcggagc ctcccgcgcg gctacgtcgt cgcgagcgcc gagcgctcca ccgtcgggtc
      361 cacgccggaa ggcgtcacgc tccacctccg ccagatcgat accgacgcgg cgggcgaacc
      421 attgaccttg catgtcgagc acgacaccgg gatgccacgt ccctggtcgt cggaacaagg
      481 atcgatcacg ttcgcgggtc tcgaaggccg atggacgccg agccgctccc agctcgaatg
      541 ggtcgaccgc ggcgtctatc actccctcca gggagcgatg gatctccgat cgttgatcga
      601 gatcgccggc gcgatcgccg cggtgaggac gggagccggg tgagacgctc gctccgatac
      661 ggcgccggcg gcctcacggg cgtcgcactc ggtatcgtgc tcaccctcgc gatccaggcg
      721 gcgtcgctcc catccacccc cgaagcgccg acggggccgt cgctgctcct cacgcgacca
      781 gcgccgccgg agacgttcct cgcgtgggtg ccgcgtggcc ttcccgacac cttcgccgcg
      841 cgggtggctc ggcaaccgga gatcgggagg ctcaccgtcg tcgccgagga caacgtgtgg
      901 ctcgcccgct cgtggagcgc ggcgggtgag ctcgtcgacc gccccgtgcg tccgtaccgg
      961 atcccgctcg acgtcgccgc cgtcgacccg gagacgttcg cgccgttcct ccccccggcg
     1021 gaccaggcga cgctcgcggc gatcgaggac ggcgagggga tcctcggatc gacctctgcc
     1081 tccgtccgac ggctgggacc gggcgggctg ctggagctcg ggcgagggaa actcatccgg
     1141 atcgccgcgg tctggccgga cgagctggtc ggtgcttcgg agctcctcgt gtcgaaggag
     1201 acggggcggc ggatcggcgt cacacgcgag cggtacgtgt tggtccgtcc ggccgcgggc
     1261 ggttcgatca catccggttc gctccggcgc gccatccagc cgctccttcc tcgggacctc
     1321 ggcgtcttcc gccgcgtcca ggtgcgttcg ccgggagaga ccccgtactt ccgcgcgggc
     1381 gatgcggtgc tcccccccgt cctgctgaag gcgttcttcg gtgagttcgc ggcgcgaccg
     1441 gcgccggggc agcccggctc cctcgaggtc gacccggagt ggatcaggac gcacctcgtc
     1501 acgaccggga tcccgctcct cggacgggtg acgtgccaca cggggatcgt cccgcagctc
     1561 cgcggcgcga tgcgggagct ccgcgatgcc ggcgccgcag gcgtgatccg gtccttccac
     1621 ggctgtttcg tcccgcggtt catcagtcgc gaccccacgg ccctgctctc ccaccattcc
     1681 tggggggtcg cgttcgacgt gaacctggtc ggcaactact acggcgtgaa gccccaccag
     1741 ccgccccggc tccggcggac gctcgctcgg tggggattca tctggggcgg gacgttcgtg
     1801 gtccccgacg ggaatcactt cgagtaccgg cggctgccga agaggggcta gcccggatca
     1861 gccgttcccg acgagcgcgc tgaggcgatc ggtgggcgag aggacgtcgg gatcgacccg
     1921 gcagtcgacc acggccggcc ggccgcacgc ccgagccgcc gtgagggcgg cgatgagctc
     1981 gggggccctc gagacggacc aggcctcggc accgtatccc cgagccaccg cggcgatgtc
     2041 cacgtcgccg atgtcgactc ccaccgtgcg cccgtacgcg cgcgcctggt gcaacgcgat
     2101 cgtgccgtag aggccgttgc ggaacaccac ggtggtgaag ggcacgtcgt ggcggaccgc
     2161 cgtttccagc tcctgccccg tcatcaggaa tccaccgtcg cctgccagga cgacgacctc
     2221 gctcgacgga tccgcgagct tcgcgccgac gccgcccggg acgccgtacc ccatcgcgcc
     2281 gctcgtgggt ccgatctgcg tgcgtggatg cgagaaccgc cagtagcggt gcgcgaagat
     2341 cgagaagttc ccggcgtcgt tcacgaggac gaccgagggc gggaacacct gcgcgagggc
     2401 cgcgatcacg gccgccgggt ggagcggacc gtcggtcgcg tcggggaggg tgctcgccct
     2461 gaggaacgcg ccgtgcgcgg cggtccagtc gcgatcggcc ggcgtatccg gagcgtggtc
     2521 gaggatcgcc gtcaacgcct cgcgcgcgtc cgcgaccaga ccgagctcca cgggtaccac
     2581 cgcgccgacg ctgttcggct ccacgtcgat ctggatcacc cgctgatcgg cactcggcag
     2641 ccggtagccc tgggtcgtga tctcgctcag ccggcagccg acgacgaaca cgacgtcggc
     2701 gtcctccagg gcccgcagcg tctccggcgg cgtggagagg acgagcgggc cgcagtagtt
     2761 cggatggtcg ttggggaaga cgtcctggcg ccggaagctc gcgtagacgc ccaggccgaa
     2821 ccgctccgcg gtggtgacga gcgcatcccg cgcgtccctc cccccgcctc ccacgatcat
     2881 cacgggcgcc accgcgtccc ggaggacgtc cgcgatgcgc tcgctcgtgc gttcgctcgg
     2941 cgtgggcctg tcccggtcga ccacaccggg gaggtggctc tgctccgggc ccgcgtcgcc
     3001 gtcgaagagg tccgctggga cggcgatcat caccggaccc ggccgcccac tcgtcgcgac
     3061 gtggatcgcc cggccaacca actccgggag ccgttcgctg gacggggccg tgacagccca
     3121 cttcgtgatc tcccggtaga acgacgggag gtcgacctcc tggaacgcct cccggccgag
     3181 gaacgccgac tccacctgcc cgaggatcac gaccatcggt gtcgagtcct gggaggccgt
     3241 gtgcacgccg atcgagaggt tcgccgcgcc gacgcccctc gtcgccatcg caagtgccgg
     3301 ccgtccggtg accttcgcgt cggcctcggc catgaacgcg gcgccggact cgtgccgcgt
     3361 ggagatcagc gcgacgtccg ggtgacggtc gcaggcatcg agcagcggga ggaagctctc
     3421 gcccggaacc gtgtaggcgc gccggacgcc ggcggcggcg agctgcgcga cgatccgctc
     3481 cgataccgtc acgcgtccca ccgcctccca ggtcgaagac ggggaggata cgccgcagcc
     3541 gggaccgaga cgggctcgtg aggacctgtg gagctactcc ttgatggcct caccctccgg
     3601 ggacaccacg taccagacgt cgctcacggc ctgcccgttg atgtcgtccg gcgtctcgtc
     3661 ggcagcgaag tagtacagcg gatggccgtc gagggtgacc tggacgctac cgtcctcgcg
     3721 ctccgtcgtg ccgaggagcg aggcgtcgat cccttcgccg gcggcgggat caccctccac
     3781 ggtgagcggc ggccagttgt cctcgcactc gtcgtagcac gtgctgtcgc tgcccgtgtc
     3841 ggcgaggaac acgtagagcg tgcgtccctc ggcagcatcg accacgatct ggccgaagtc
     3901 ggaatcctcg acggcgaccg tcgcctcggc gggctcctcg gacggctccg acgactccgt
     3961 cggcggggat gttccgtcgg tgggctcgac gtcctccgag cacgcgccca tcacgagcgg
     4021 gacgagcgcg gcgaccgcga gcaccttgat cttgccagac accatccgag cctccttcgt
     4081 cgtcgtcggg aggtctacgg atgggtgggg gccccggttc gctg
//
LOCUS       DASUVE010000139         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_820566, whole genome shotgun sequence.
ACCESSION   DASUVE010000139 DASUVE010000000
VERSION     DASUVE010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820566"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..374)
                     /locus_tag="VF036_02895"
     CDS             complement(60..374)
                     /locus_tag="VF036_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX6264209.1"
                     /translation="MIAHITTLAVLAQDAGGRGDDIDIASILGYVVVGAIVGVLARVL
                     VPGREDMGVIATIVLGIIGAVIGGWVAGAIFEDTAGVDWIASIVAAIGLVLLARAVRT
                     RA"
     gene            497..733
                     /locus_tag="VF036_02900"
     CDS             497..733
                     /locus_tag="VF036_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264210.1"
                     /translation="MHERRIEHWRLICRACGDAAAVTNERRFCACGRSSARPTPDGGV
                     LVAGPGLAVVGRTEAGPWEIARGDRVTRPRGVVA"
     gene            complement(737..1501)
                     /locus_tag="VF036_02905"
     CDS             complement(737..1501)
                     /locus_tag="VF036_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX6264211.1"
                     /translation="MTDDAGRSQAHDPAEDEALELFRQLPDPAARERLTERYLPLAEH
                     LAGRFVGRGEARDDLVQVAGLGLVNAIDRFDPDRGVRFTTFASITILGELRRHFRDKG
                     WSVRVPRGLQEAALELNRTLGRMWQELGRSPTIAELAGGSGLPEETIIEAMDAVQAYS
                     TSSLDAPVGEDEGASMGDLIGDEDPSLAVSDEWMSIEPALKDLPERERRILYLRFFED
                     MTQTEIAEVVGISQMHVSRLIAAALAKVREASLGQR"
     gene            complement(1491..1895)
                     /locus_tag="VF036_02910"
     CDS             complement(1491..1895)
                     /locus_tag="VF036_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264212.1"
                     /translation="MTGTPAISVSGPAVPETLRVFRNVAASEGARDDISFDRLEELRV
                     AVDEAATLVLLAEGARTLTLDLRSDDGGLQVSVRSDGTAEDWPGDRHRSWGGKVIAAL
                     ASDVRMTVDDGCPEVGFSWLASEGARVDRRDG"
     gene            2097..2387
                     /locus_tag="VF036_02915"
     CDS             2097..2387
                     /locus_tag="VF036_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264213.1"
                     /translation="MRNPGNGITDTAGPLKARAPVRGMEPVETAPRGRRCADPGCPTT
                     LSVYNRGHHCWQHEPRRPFTLQAPRGEPIQTSDDEILASISGAQVRAHRASA"
     gene            complement(2487..2690)
                     /locus_tag="VF036_02920"
     CDS             complement(2487..2690)
                     /locus_tag="VF036_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002493030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX6264214.1"
                     /translation="MGEKTDKAKGAVKETVGEMTDDERLEREGKIDKATGAVKGAGDD
                     AKDTIEDGVDALRRKARDLNDDR"
     gene            complement(2721..>2813)
                     /locus_tag="VF036_02925"
     CDS             complement(2721..>2813)
                     /locus_tag="VF036_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019062196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HEX6264215.1"
                     /translation="RDQLYAEAKRRNIRGRSRMTKAQLQRAVDR"
BASE COUNT          401 a         1066 c          940 g          406 t
ORIGIN      
        1 cgggcggagg tcgccgtcct gggactgctc gagggtacgg gtgacccgag gccgtagcct
       61 caggcgcggg tccgcaccgc ccgtgcgagc agcaccagcc cgatggcggc cacgatcgac
      121 gcgatccagt cgaccccggc cgtgtcctcg aagatcgcgc cggcgaccca tccgccgatg
      181 accgcgccga tgatgcccag cacgatcgtc gcgatcacgc ccatgtcctc acgcccgggc
      241 acgaggaccc gagccaggac gccgacgatg gcgccgacga ccacgtagcc gaggatcgaa
      301 gcgatgtcga tgtcgtcacc ccgtccgccc gcgtcctgcg cgagtacggc caaggtcgtg
      361 atgtgtgcga tcacagcgtg ctccttccgt ctgggaaacc gatgcatcct ggttcccgta
      421 tccgaccgat gctcaaaccc cccgccgtgc gcacgtcacc cgtttgtcgg cccgggtgcg
      481 cgggtacgcc gccgccatgc acgaacgtcg tatcgagcac tggcgcctga tctgccgcgc
      541 gtgcggcgac gccgccgcgg tcacgaacga gcgtcggttc tgtgcctgcg ggcgttcgag
      601 cgcgcgaccg acgcctgacg ggggcgtcct ggtggccggt cccggtctcg cggtcgtcgg
      661 tcggaccgag gccggcccgt gggagatcgc gcggggcgac cgggtgacgc gcccacgcgg
      721 ggtcgtggcc tgagggtcag cgttgaccga gggaggcctc gcgcaccttc gcgagcgccg
      781 cggcgatcag acgcgagacg tgcatctgcg agatcccgac gacctcggcg atctcggtct
      841 gcgtcatgtc ctcgaagaac cgcaggtaca ggatcctgcg ttcccgctcc ggcaggtcct
      901 tcagggccgg ctcgatcgac atccactcat cggagacggc gagcgacgga tcctcatcgc
      961 cgatcaggtc gcccatcgaa gctccctcgt cctctcccac gggcgcgtcg agcgacgagg
     1021 tcgagtaggc ctgcaccgcg tccatcgcct cgatgatcgt ctcctcgggc agtccgctgc
     1081 cccccgcgag ctccgcgatc gtgggtgagc gccccagctc ctgccacatg cggccgaggg
     1141 tccggttgag ctccagggcc gcctcctgca gacctcgcgg aacccgcacg gaccacccct
     1201 tatcccggaa gtgccgccgc agctcgccga ggatcgtgat cgaggcgaag gtggtgaacc
     1261 tgacaccgcg gtccgggtcg aagcggtcga tcgcgttcac caggccgaga ccggcgacct
     1321 ggaccaggtc gtccctcgcc tcgccccgac cgacgaagcg ccctgccaga tgctcggcga
     1381 gcgggaggta ccgctcggtc agccgttcgc gcgccgcggg gtccggcagc tgacggaaca
     1441 gctcgagcgc ttcgtcctcg gcgggatcgt gcgcctggga acgcccggcg tcatccgtca
     1501 cgtcggtcca cgcgggcgcc ctcgctcgcg agccaggaga accccacctc cggacagccg
     1561 tcgtccacgg tcatccggac gtcggacgcc agggccgcga tcaccttgcc accccacgag
     1621 cggtggcggt cgccgggcca gtcctcggcg gtcccgtccg agcggacgct gacctgcaga
     1681 ccgccgtcgt cggaccgcag gtccagcgtg agcgtgcgcg ccccctcggc cagcagcacc
     1741 agggtggccg cttcgtcgac cgccacacgg agctcctcga gacgatcgaa ggagatgtcg
     1801 tcgcgggccc cttcgctcgc tgccacgttg cggaacacgc ggagggtctc cgggaccgcc
     1861 ggtcccgaca ccgagatcgc cggcgtcccg gtcacctccg cgtcccctcc tgggcgagct
     1921 ccatccgcca cacgatagcc cgaggatcgg cgggcgatcg ccgggagtgg aaggcgccga
     1981 acacgccgcg agctacccgc tcaaccgctt gcgcaaacgg atcggacgca ccgcgtttag
     2041 ccgtgcccgg ctgtgggcaa cggacctcac gagccacccc cgagagagga gcgatcgtgc
     2101 gcaaccccgg caacggcatc accgacacgg cgggacccct caaggcacgc gcacccgtcc
     2161 gcgggatgga gcccgtcgag acggcgccac ggggacgccg gtgcgccgat cccggctgtc
     2221 ccaccacgct gtccgtgtac aaccgcgggc accactgttg gcagcacgag ccgcgccgac
     2281 cgttcaccct gcaggcccct cgcggcgaac cgatccagac cagcgacgac gagatcctcg
     2341 cgagcatctc cggcgcgcag gtgcgggcgc accgagcgag cgcgtaagac cggcctcgcg
     2401 gccaccggca cggacggggc ccgggtcaga tgacccgggc cccggaacgt gcggtgcggg
     2461 acggcgtcgg tcccgtcagg cgcgcctcac cgatcgtcgt tcaggtcgcg ggccttgcgc
     2521 cggagcgcgt cgaccccgtc ctcgatcgtg tccttggcgt cgtcaccggc gcccttcacg
     2581 gcaccggtcg ccttgtcgat cttgccctcg cgctcgagtc gctcatcgtc ggtcatctcg
     2641 ccgacggtct ccttcacagc gcccttggcc ttatcggtct tctcacccat cgtgactcct
     2701 ctcctgccgc ctcggcggcc tcatcggtcc acggctcgtt gcagctgtgc cttggtcatc
     2761 cgggaacggc cgcggatgtt cctgcgcttc gcctcggcgt agagctggtc ccg
//
LOCUS       DASUVE010000140         3964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1010333, whole genome shotgun sequence.
ACCESSION   DASUVE010000140 DASUVE010000000
VERSION     DASUVE010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010333"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VF036_02930"
     CDS             <1..339
                     /locus_tag="VF036_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264216.1"
                     /translation="AGRDLAWPSITTDGNGKLWINYARGGATECLAAYTSVVQPGEQA
                     AASILIKPGSGRYEFSPGEVERWGDYTAVARDPVTPTRVATYGAYAFNDGGGAVTQLW
                     RQVIASVEDT"
     gene            379..2088
                     /locus_tag="VF036_02935"
     CDS             379..2088
                     /locus_tag="VF036_02935"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX6264217.1"
                     /translation="MRVSTLFARTLRDDPAEAEVASHRLLLRAAFIGKTAAGIYTTMP
                     LGLRTTAKIERVVREEMDAAGSQELRMPIVLPAEPWRATGRYHAYGDLMFRLEDRHGR
                     EFVLGPTQEEVVAELAQHELPSYRDLPSNVYQVEWKYRDEFRPRYGLLRGREFLMKDA
                     YSLDRDEVGMGESYQAMYDAYVKVFDRLGLDYVIVEADPGQIGGGVNHEFMALADVGE
                     DLFVRCENGDYLADTEAATPRPPDAATDEELPPLTEVETPGAVTIELMSEQMGLPAEA
                     TMKCILFDVAGRSVAVLVPGDREVNEEKLARVHFPARVRPFEDDDFAERGFAKGFVGP
                     QGLGSDVTIFADPAIRGRGHWATGANRPDVHVTGATLDRDFRVDRWQDLVQFHEGDRC
                     PVDGGRLEIARSIVVGHIYQLGTYYSAPLQATFQDEDGSQKPFVMGSYGIGITRIMSA
                     AVEQNHDDAGIRWPKLLAPFEVAIIMANADAPAVTEHAERIYHELTDRGIDVVLDDRE
                     ERAGVKFADADLVGYPVQVVVGARGIEAGTVDLKLRSTGHRSQVLLADAAEAAADLLT
                     TAP"
     gene            2226..2681
                     /gene="rimP"
                     /locus_tag="VF036_02940"
     CDS             2226..2681
                     /gene="rimP"
                     /locus_tag="VF036_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX6264218.1"
                     /translation="MDAEALVRPVIEGEGLDLVDVTFGRESGRRILRVTVDRDGGVDL
                     DTISQISQKVSRRLDLEGFEPGPYALEVSTPGIERPLRRPQDFRRAVGERVQVKTEGG
                     VLVGELRAAEDDAIRIAAAEGERLVSLEEVAAAKTVVDWDEELKKGGDR"
     gene            2678..3943
                     /gene="nusA"
                     /locus_tag="VF036_02945"
     CDS             2678..3943
                     /gene="nusA"
                     /locus_tag="VF036_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX6264219.1"
                     /translation="MNAEMIAALRELEREKGIAFETILGGLQEAMASAYKAHWKQEHP
                     EADDEFVGFRAVIDPETGDLRMWQQELEEVDSPPAEGSDEPGVEMKVVSEAEVEVTDD
                     FKGRIGAQTAKQVIFQKLRDAEREMTYEEFAGREGDVVTGIVQQQERRYTLLDLGKVE
                     ALLPQGEQVPSEPLRHGERLKAYITEVRRGTKGPQIVVSRTHPGLLKSLFALEVPEIN
                     EGIVEIMAVAREPGHRSKIAVASNEPAVDPVGACVGPKGSRVRMVVNELRGEKIDVVP
                     WSDESATFVANALQPAKVREVTVNPETQTALVIVPDYQLSLAIGREGQNARLAARLTG
                     WRIDIKSESQAAEPTPAPAPVSAEEPAAEEPPAPESGAAASETGPAEAPEDAQAATVE
                     EVATGAGAEPTPEESEPVEAVPAAPAEAE"
BASE COUNT          662 a         1275 c         1470 g          557 t
ORIGIN      
        1 gccgggcgcg atctggcatg gccctcgatc accaccgacg gcaacgggaa actctggatc
       61 aactacgccc gcgggggtgc gacggaatgt ctcgccgcct acaccagcgt cgtgcagccg
      121 ggggagcagg ccgcggcatc gatcctcatc aagcccggtt ccggccgcta tgagttctcg
      181 ccaggtgagg tcgagcggtg gggcgactac acggccgtcg cgcgggatcc cgtgaccccc
      241 acccgggtgg cgacgtacgg ggcctacgcc ttcaacgacg gaggcggggc ggtaacccag
      301 ctctggcggc aggtgatcgc ctcggtcgag gacacgtaag cggcgggagc cggcaggtcc
      361 gccgctatcc ttgccgccat gcgggtttcc acgctgttcg cacgcaccct ccgggacgac
      421 ccggcggagg cggaggtcgc ctcccatcgc ctcctgctcc gcgccgcgtt catcggcaag
      481 accgcggccg ggatctacac gacgatgccg ctcggccttc gcaccacggc gaagatcgag
      541 cgggtcgtcc gcgaggagat ggatgccgcc gggtcgcagg agctccggat gccgatcgtc
      601 ctgccggcgg aaccctggcg ggcgacgggt cgttatcacg cctacggcga cctgatgttc
      661 cggctcgagg accggcacgg tcgcgagttc gtgctcggcc ctacccagga ggaggtcgtg
      721 gccgaactcg cacagcacga gctcccgtcg taccgggacc tgccctcgaa cgtgtaccag
      781 gtcgaatgga agtatcggga cgagttccgc ccgcggtacg ggctcctccg cggtcgcgag
      841 ttcctgatga aggacgcgta ctcgctggac cgggacgagg tcgggatggg cgagtcgtac
      901 caggcgatgt acgacgccta cgtcaaggtc ttcgatcggc tcggcctcga ctacgtgatc
      961 gtggaagccg accccggtca gatcggcggc ggcgtgaacc acgagttcat ggcgctcgca
     1021 gacgtgggcg aggatctctt cgtccgctgc gagaacggcg actacctcgc ggacacggag
     1081 gcggcgaccc cgcgccctcc cgacgcggcg accgacgagg agctaccgcc ccttaccgag
     1141 gtcgaaacgc ccggcgctgt gaccatcgag ctgatgtcgg agcagatggg cctgccggca
     1201 gaggcaacga tgaagtgcat cctgttcgat gtggcgggtc gctcggtcgc ggtgctcgtg
     1261 cccggcgacc gagaggtcaa cgaggagaag ctcgcccgcg tgcacttccc cgcgcgcgtc
     1321 cggccgttcg aggacgacga cttcgccgag cgggggttcg cgaaggggtt cgtcggtccg
     1381 cagggcctcg gctccgatgt cacgatcttc gcggatccgg cgatccgcgg acgcgggcac
     1441 tgggcgacgg gcgcgaatcg cccggatgtg cacgtgaccg gcgcgacgct cgaccgggac
     1501 ttccgcgtcg accgatggca ggacctcgtg cagttccacg agggcgatcg ctgtccggtc
     1561 gacggcggac ggctcgagat cgcgcgctcg atcgtggtcg ggcacatcta ccagctcggg
     1621 acgtactact cggcaccgct gcaggcgacc ttccaggacg aagacggttc gcagaaaccg
     1681 ttcgtgatgg ggtcctatgg gatcgggatc acgcggatca tgtcggcggc cgtcgagcag
     1741 aaccacgatg acgccgggat ccgttggccc aagctcctgg ctccgttcga ggtcgcgatc
     1801 atcatggcga acgccgacgc cccggcggtc acggagcacg ccgagcggat ctaccacgag
     1861 ctcacggatc gcgggatcga tgtggtgctc gacgaccggg aagagcgggc gggggtcaag
     1921 ttcgccgacg cggacctcgt cggctacccc gtgcaggtgg ttgtcggcgc gcgcggcatc
     1981 gaggccggga cggtcgacct gaagctgcga tccaccggtc atcggtcgca agtgctactg
     2041 gcggacgcgg ccgaggccgc cgccgacctg ctcaccacgg ctccctgagg gttcgcgctc
     2101 ctccgatctg aggaggcctg tctatactgg ctggtcgagg accaactgca cggccggaag
     2161 tgggctcccc gcccactttc tttgtggagg ggcgaggccg ctcgaataga gggagaggag
     2221 ggaacgtgga cgcggaggcg ctggtcagac ccgtgatcga gggcgaaggc ctcgacctcg
     2281 tcgacgtgac cttcggccgt gagagcggcc gccggatcct ccgcgtgacg gtggatcgcg
     2341 acggtggtgt cgacctggac acgatctcgc agatctctca gaaggtgtcg cgccggctcg
     2401 atctcgaggg tttcgaaccg ggtccgtacg cgctggaggt atcgacgccc ggcatcgagc
     2461 gacccctccg acgcccgcag gacttccggc gagccgtcgg tgagcgtgtc caggtgaaga
     2521 ccgagggtgg cgtgctggtc ggcgagctcc gcgcggcgga ggacgacgcg atccggatcg
     2581 cggcagccga gggtgagcgg ctcgtgtccc tcgaggaggt cgccgcggcc aagacggtcg
     2641 tcgactggga cgaagagctg aagaaagggg gcgaccgatg aacgcggaga tgatcgccgc
     2701 gttgcgggag ctcgagcgcg agaagggcat cgccttcgag acgatcctgg ggggtctcca
     2761 ggaggcgatg gcgtcggcct acaaggcaca ctggaagcag gagcatcccg aggccgacga
     2821 cgagttcgtc gggttccgcg ccgtgatcga tcccgagacg ggcgatctcc gcatgtggca
     2881 gcaggagctc gaagaggtgg actccccccc cgcggagggg agcgacgagc cgggggtcga
     2941 gatgaaggtg gtctccgagg cggaggtgga ggtcacggac gacttcaaag gacggatcgg
     3001 cgcccagacc gcgaagcagg tcatcttcca gaagctccgc gacgccgagc gagagatgac
     3061 ctacgaggaa ttcgccggcc gcgagggcga cgtcgtcacg gggatcgtcc agcagcagga
     3121 gcgtcgatac acgctgctgg atctcgggaa ggtggaggcg ctgctgccgc agggcgagca
     3181 ggtcccgtcg gagcccctgc ggcacgggga gcggctcaag gcgtacatca cggaggtccg
     3241 tcgggggacg aagggcccac agatcgtcgt ctcacggacg caccccggac tgttgaagtc
     3301 gctgttcgca ctcgaggtgc ccgagatcaa cgaggggatc gtcgagatca tggccgtggc
     3361 tcgcgagccg ggccatcgct cgaagatcgc ggtggcctcc aacgagcccg cggtcgatcc
     3421 cgtcggcgcg tgcgtcgggc cgaagggctc acgcgtgcgc atggtcgtga acgaactccg
     3481 cggcgagaag atcgacgtgg tcccgtggtc cgacgagtcg gcgacgttcg tcgcgaacgc
     3541 attgcagccg gcgaaggtca gggaggtgac cgtgaacccc gagacccaga cagcgctcgt
     3601 gatcgtgccc gactaccagc tctcgctcgc gatcggccgg gagggccaga acgcccggct
     3661 cgccgctcga ctgacggggt ggcggatcga catcaagtcc gagagccagg cggccgagcc
     3721 cacccccgcg ccggccccgg tctcggccga ggaacctgct gcggaggaac cacctgcgcc
     3781 ggagagcggg gcggcggcat ccgagacggg accggcggaa gctcccgagg atgcgcaggc
     3841 agcgacggtg gaggaggtgg ccacgggcgc cggcgccgag ccgacacccg aggagtcgga
     3901 gcccgtcgaa gcggtcccgg ccgcgcccgc ggaggcggaa tgaggaacca tggcgcgcga
     3961 gccg
//
LOCUS       DASUVE010000141         3189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_634382, whole genome shotgun sequence.
ACCESSION   DASUVE010000141 DASUVE010000000
VERSION     DASUVE010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634382"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..385
                     /gene="ndhC"
                     /locus_tag="VF036_02950"
                     /pseudo
     CDS             59..385
                     /gene="ndhC"
                     /locus_tag="VF036_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
     gene            459..953
                     /locus_tag="VF036_02955"
                     /pseudo
     CDS             459..953
                     /locus_tag="VF036_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            974..1474
                     /locus_tag="VF036_02960"
     CDS             974..1474
                     /locus_tag="VF036_02960"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX6264220.1"
                     /translation="MKAAALADRIRGAFDDVLLARDEVTLLVGREALVDTLVRLRDQP
                     DLSFGFLSSLTATDHPGRDPRFWVAYELRSIEHAHRMRVKVGLAGDDPRLPSITALFP
                     TANWHEREVWDLFGLVFDGHPDLDRILLPDDWEGHPLRKDEELGGVGTQYHGAFIPPV
                     DERTMP"
     gene            1474..2793
                     /gene="nuoD"
                     /locus_tag="VF036_02965"
     CDS             1474..2793
                     /gene="nuoD"
                     /locus_tag="VF036_02965"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012832321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEX6264221.1"
                     /translation="MAIGHDSGVGEEMEQARDELRSRPEIRDHVTGEVRQETMIINMG
                     PQHPSTHGVLRLLLELDGETVIRCKPIVGYLHTGIEKNTEYRTWVQGVAYVTRADYLS
                     PFFNELAYCLSVERLLGIEAPPRAQVLRVLLCELNRIASHLVWVATGGMELGAVSVML
                     YGFRERELILDIFEAVTGLRMNHSFIRPGGVIMDLPPDGARRIATFLDTMPARIDDYE
                     RLLNDNPIWLERNVGVGILSAEDALAFGVTGPLLRAAGMSVDLRKDEPYSGYETYDFD
                     VPVRTEADSYARYLVRIAEMRESLRIVEQALQRLQEPGPVMVEDPKVGWPVKLSVGPD
                     GIGNDEAYLHHIMEESMEALIHHFKMVTQGVRVPEGEVYTTVESPRGELGYYVVSANE
                     YRPYRVKIRDPSFVNLQAVPTMVEGGLIADAIAVIASLDPVMGGVDR"
     gene            2793..>3189
                     /locus_tag="VF036_02970"
     CDS             2793..>3189
                     /locus_tag="VF036_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX6264222.1"
                     /translation="MAVFEGPRLEEAKEILRRYPEGRERSAIMPLLYLAQSVEGHISR
                     DGLREVAELLGRTTAEVEAVATFYTMYRLRPTGTHVVSVCTNLACMLRGGREVLDAAH
                     EAAGMRHGDERSDDGMFTVHEEECLGVCDF"
BASE COUNT          479 a         1051 c         1121 g          538 t
ORIGIN      
        1 gcacggctcc ctcgtctccg tggtcgcggc gggactgagc gactacctgc cgatcctcat
       61 gttggcggtg ttggcgatct tcttcgcggt ggcgtcgctc ggagcgtcgg cggtgctccg
      121 cccccgtcgg ccgaaccggc cgaagctgtc cgcctacgag agcgggaacg atccggtccg
      181 gctgccgggt ggagaacgtt tcagcgtcaa gttctacgtc gtggcgatgc tgttcatcat
      241 cttcgacatc gagacgatct tcctgttccc gtgggccgtc acgttccgcc agctcggtct
      301 attcgggttg atcgagatgg cgatcttcat cgcgctcgtg ttcgtcgcgt acgtgtacat
      361 ctggaagaag ggcggtctcg actggtccgc gacgcccccg gagctgctgg acgatgaggt
      421 cggcgatgaa ccggtggcgg cgggggaggt gtcgctcgat gcccgttgag cgcgtcgagg
      481 ggcacgtcac cggtcgaacc cccgaggagg tcgaggacga gatccgcaag ggcgtcctgc
      541 tgaccacgct ggatcgcgcc gtcagctggg cgcgcacgca gtcgatgtgg ccggcgacgt
      601 tcggactcgc ctgctgtgcg atcgagatga tgtccgccgg cgccgggcgc tacgacctct
      661 cgcggtgggg tatggagctg ttccgggcct cgccccgcca agccgacctg atgatcgtgg
      721 cggggcgggt ctcccagaag atggcgcccg tcctgcgccg cgtctacgac cagatgcccg
      781 agccgaagtg ggtcgtctcg atgggagtct gcgcctccgc cggcggcatg ttcacgaact
      841 acgcgatcgt gcagggtgtc gacacgatcg tgcccgtcga catctacgtc ccgggctgcc
      901 cgcccaagcc ggagatgctc atgttcggca tcttgaagct gcaggagaag gtccggacga
      961 cggggacgat ccgatgaagg cggcggcgct cgccgaccgg atccgggggg cgttcgacga
     1021 cgtgctgctc gcgcgcgacg aggtcacgct gctggtcggt cgggaggcgt tggtggacac
     1081 gctcgttcgc ctccgggatc aacccgacct gtcgttcggg ttcctgtcga gcctcaccgc
     1141 cacggaccac ccgggcaggg acccccgctt ctgggtcgcc tacgagctcc ggtcgatcga
     1201 gcatgcccac cggatgcgcg tgaaggtggg tctcgcggga gacgatccgc gcctcccgtc
     1261 gatcacggcg ctcttcccga cggcgaactg gcacgaacgc gaggtctggg acctgttcgg
     1321 tctcgtgttc gacgggcatc cggacctcga tcgcatcctg ctccccgacg actgggaggg
     1381 gcacccgctg cgtaaggacg aggagctcgg cggcgtcggc acgcagtacc acggtgcgtt
     1441 catcccgccc gtcgacgaac ggacgatgcc ctgatggcga tcggccacga ctcgggggtc
     1501 ggtgaggaga tggagcaggc tcgcgacgag ctccggagcc gtccggagat ccgtgaccac
     1561 gtcaccggcg aggtccgcca ggagacgatg atcatcaaca tggggccgca gcacccctcg
     1621 acccacgggg tgctccggct cctgctcgag ctggacggcg agacggtgat ccgatgcaag
     1681 ccgatcgtgg ggtacctgca caccggcatc gagaagaaca ccgagtaccg cacctgggtg
     1741 cagggcgtcg cgtacgtgac ccgcgccgac tacctctccc cgttcttcaa cgagctcgcg
     1801 tactgcctgt cggtggagcg gctcctcggg atcgaggcac cgccccgcgc gcaggtcctc
     1861 cgcgtgctgc tctgcgagct gaaccggatc gcttcgcacc tggtgtgggt ggcgaccggc
     1921 gggatggagc tcggcgccgt ctccgtgatg ctctacgggt tccgtgagcg cgagctgatc
     1981 ctcgacatct tcgaggctgt caccgggctc cgcatgaatc attcgttcat ccggcctggc
     2041 ggcgtgatca tggacctgcc gcccgacggc gcccgcagga tcgcgacgtt cctcgacacg
     2101 atgcccgcgc ggatcgatga ctacgagcgg ctcttgaacg acaacccgat ctggctggag
     2161 cgcaacgtcg gcgtgggcat cctgtccgcc gaggacgcgc tcgcgttcgg cgtgaccggg
     2221 ccgctccttc gcgcggccgg gatgtcggtg gacctccgca aggacgagcc gtacagcggc
     2281 tacgagacct acgacttcga cgtccccgtg cggaccgagg cggattcgta cgcgcggtac
     2341 ctggtccgga tcgcggagat gcgggagtca ctgcggatcg tcgagcaagc gctccagcgg
     2401 ctccaggagc ccgggcccgt gatggtcgag gaccccaagg tcggctggcc ggtgaagctc
     2461 tcggtgggac ccgacggcat cggcaacgac gaggcgtacc tccaccacat catggaggag
     2521 tcgatggagg ccctgatcca tcacttcaag atggtcaccc agggcgtccg cgtccccgag
     2581 ggcgaggtct acacgaccgt cgagtccccc cggggagagc tcggctacta cgtcgtctcc
     2641 gccaacgagt accggccgta ccgcgtgaag atccgcgacc cgtcgttcgt gaacctccag
     2701 gccgtgccca cgatggtgga gggcggcctg atcgccgacg cgatcgcggt gatcgcctcc
     2761 ctcgaccctg tgatgggagg cgtcgatcgc tgatggccgt cttcgagggt ccacggttgg
     2821 aggaggcgaa ggagatcctc cgccgctacc ccgaggggcg ggagcgctcc gcgatcatgc
     2881 cgctgctcta cctcgcgcaa tccgtcgagg ggcacatctc tcgcgacggc ctccgggagg
     2941 tcgcggagct cctcggccgc acgacggcgg aggtggaggc cgtcgccacg ttctacacga
     3001 tgtatcgcct tcgtccgacc gggacgcacg tcgtgagcgt gtgcacgaac ctggcctgca
     3061 tgctccgcgg cggtcgggag gtcctcgacg ccgcgcacga ggcggccggg atgcgacacg
     3121 gtgacgaacg gtcggatgac gggatgttca ccgtgcacga ggaggagtgc ctgggcgtgt
     3181 gcgacttcg
//
LOCUS       DASUVE010000142         4170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_69375, whole genome shotgun sequence.
ACCESSION   DASUVE010000142 DASUVE010000000
VERSION     DASUVE010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69375"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VF036_02975"
     CDS             <1..421
                     /locus_tag="VF036_02975"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6264223.1"
                     /translation="EFYEIREALESVAVAHAIPNLRKDVARDLGALIDRMRRTEDARK
                     WLKLNDQFHLRLYEGAARPRLSGLIDSLRDASAPYIYMFVASRPPSQRANEQHQEILD
                     ACVRGDAAGAKRAIREHLRSAARDLAAFLAAQENTTP"
     gene            597..1655
                     /locus_tag="VF036_02980"
     CDS             597..1655
                     /locus_tag="VF036_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX6264224.1"
                     /translation="MRTRSLVRLVAATCALALIAAACGEDGGTGGDGERSIVRFAFSP
                     DPVIDWMNDKGIIPEYEEQYNVRLVTTSTWDEFTFFAGGHGDIVSMATYETPLLEQET
                     GVDTVTFGSYNNLRVPVFVRSDSDYETFEDLKGTTVAVPSAVSSTIVWGMFVKDWYGL
                     DFCIERPDGSDCADYEVIEGDHFANMELLIRGDIEACVCIPEAGFPYWRSGEIRALYE
                     PTPSPWELYADMYAPGHKGVNGNNFVARKDWFDSHPNEVAFFLALWERGIQEWLENKE
                     EIIRTYPQHFAVEDEADVDFAVEFMEEHDFFTDTVYMDEAWVEDETGVYDLMKETGWM
                     DPDAEIPEFQVVEPSEPE"
     gene            1747..2586
                     /locus_tag="VF036_02985"
     CDS             1747..2586
                     /locus_tag="VF036_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6264225.1"
                     /translation="MATQDVEAAAPPVRIDTEPDGPTPERPRRQRYLLWRIAGYILFI
                     GAWAFVSTLTEPYILPSPVAVLRKMGEVLARDDFWINVSATLRNLAIGFTLAFVIGTA
                     IGIAMGRSAYWDAFFRDYVMLTLTTPGLVFALVCVIIFGLVEIGPIVAIVLTSFAHIT
                     VNVVEGVRAIPRDLMDMATAYGVDRRTRIRNIVLPAVAPFLFTAVRYGFAIAWKITAL
                     TELFGQTDGVGIQIRVEFLFFDIAGVLAWAFFLVGFALVMERLVLQRLERRFFRWRPQ
                     AFA"
     gene            2595..3458
                     /locus_tag="VF036_02990"
     CDS             2595..3458
                     /locus_tag="VF036_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6264226.1"
                     /translation="MGGTMEQTVGARKGTVPARRSWFERTFLTPRAARITAPVLFLVA
                     WQLIAPLIPSVILPMPAEVVEAMWEQLRDGTLVEAFSVSLLRLAIGFALALAVGSIVG
                     LAMGLSPRIEAMLHDFVVVGLTFPYLIWGLLVAMWFGYGSIGPVLVVFIAGLPYVILN
                     MSEGVQDVSKELRDMAAAYEVPRDRVMRHLILPSLSPFFFASLRYGLANGWKGLILAE
                     VFAATSGAGWHITSMREFGDFAGVFGLAIYFSLFSIVVERLVFGRLSRRVFRWRPTLA
                     QEMSEPPELTI"
     gene            3484..>4170
                     /locus_tag="VF036_02995"
     CDS             3484..>4170
                     /locus_tag="VF036_02995"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6264227.1"
                     /translation="MATVTVTDLRKVFLGEGRDVVALDGVGFDASGHTFVSIVGPSGC
                     GKSTLLNILAGVETPTSGRADITENGTRARVGYVFQAPRLLPWRSVMDNMLFVQPEQS
                     EEVRDRAAHYLDMVGLSEHLKAWPSQLSGGMQQRVGIARAFSIEPDALLMDEPFSHLD
                     AITARGLRRELHSMWKETKKTVLFVTHDVGEAVELSDRIVILAKGGKLRQDLTVDLPY
                     PRDPADDKVAL"
BASE COUNT          666 a         1339 c         1438 g          727 t
ORIGIN      
        1 cgagttctac gagatccgcg aggcactgga gtccgtcgcg gtcgcacatg cgatcccgaa
       61 cctgcgcaag gatgtggctc gcgacctcgg agcactgatc gatcggatgc ggaggaccga
      121 ggacgccagg aagtggctca agctgaacga tcagttccat ctgcgtctct acgaaggtgc
      181 cgcgcggcct cgcctctcgg gcctgatcga cagcctgcgc gacgcctccg ctccgtacat
      241 ctacatgttc gtggcctcga ggcccccgtc gcagcgggcc aacgaacaac accaggagat
      301 cctggatgcg tgcgtccgcg gcgatgcggc gggcgcgaag cgggccatcc gcgaacacct
      361 gcgaagcgcc gcccgcgacc tggccgcgtt cctcgccgcc caggaaaaca ccacccccta
      421 gcgcgaccac acgggaccgc ctgaccgcct tgacggccgg tgactagact gcgcattata
      481 tatagtacgc tggaaaggtg ggggacgcgg aacggcgacc cggccccatg ggatccccac
      541 cgcggcctcg atcctggccg tgcgagtccc gatgggtccc gacgagggag gggcggatgc
      601 ggacgaggag tctggtacga ctcgtggcgg cgacatgcgc gctcgcgctc atcgcggcgg
      661 cgtgcggtga ggatgggggg acgggaggcg acggcgagcg atcgatcgtc cgcttcgcgt
      721 tctctccgga tccggtgatc gactggatga acgacaaggg gatcatcccg gagtacgagg
      781 agcagtacaa cgttcgtctc gtgacgacgt ccacgtggga cgagttcacg ttcttcgccg
      841 gcggacacgg cgacatcgtg tcgatggcga cgtacgagac gccgctcctc gaacaggaga
      901 ccggcgtcga cacggtcacg ttcgggtcct acaacaacct gcgcgtcccg gtcttcgtcc
      961 gatccgacag cgactacgag acgttcgagg atctgaaggg aacgacggtc gccgtcccga
     1021 gcgccgtctc ctcgacgatc gtctggggga tgttcgtcaa ggactggtac gggctcgact
     1081 tctgcatcga gcgaccggac gggagcgact gcgcggacta cgaggtgatc gagggcgacc
     1141 acttcgcgaa catggagctg ctgatccgcg gcgacatcga ggcgtgcgtt tgcatcccgg
     1201 aggccggctt cccgtactgg cggtccggcg agatccgggc gctctacgag ccgacgccat
     1261 ccccgtggga gctctacgcg gacatgtacg cgcccggaca caagggcgtg aacgggaaca
     1321 acttcgtggc ccggaaggac tggttcgaca gccatccgaa cgaggttgcg tttttcctcg
     1381 ccctgtggga gcgcgggatc caggagtggc ttgagaacaa ggaggagatc atccgcacgt
     1441 acccgcagca cttcgcggtg gaggacgaag cggacgtcga cttcgctgtc gagttcatgg
     1501 aggagcacga cttcttcacg gacaccgtgt acatggacga ggcatgggtg gaggacgaga
     1561 cgggcgtcta cgacctgatg aaggaaacgg gctggatgga tcccgacgcg gagattcccg
     1621 agttccaggt cgtggagccg agcgagccgg agtgaggtgg tagcggaacc agcccacacg
     1681 tgagctgagc gatctggtcg gcgggccgcg agcccgccgg cccgatcgga ttggagttcg
     1741 acgcaggtgg cgacgcagga cgtggaggcg gccgcacctc cggtgcggat cgatacggaa
     1801 ccggatggac ccacgccgga acgcccgcgc cggcagcggt atctcctctg gaggatcgcg
     1861 ggctacatcc tgttcatcgg cgcgtgggcg ttcgtctcga cgctgaccga gccgtacatc
     1921 ctgccctcgc cggtggcggt tctccgcaag atgggcgagg tgctcgcccg cgacgacttc
     1981 tggatcaacg tctccgcgac cctccgaaac ctcgcgatcg ggttcacgct cgcgttcgtg
     2041 atcggcaccg cgatcggcat cgcgatgggc cggagcgcgt actgggacgc cttcttccgc
     2101 gactacgtga tgctcacgct gacgacgccc ggcctggtgt tcgcgctcgt gtgcgtgatc
     2161 atcttcggct tggtcgagat cggtcccatc gtcgcgatcg tcctcacctc gttcgcgcac
     2221 atcacggtga acgtcgtcga aggcgttcgc gcgatccctc gcgatctgat ggacatggcg
     2281 acggcctacg gcgtcgatcg caggacgcgg atccgcaaca tcgtgctgcc cgcggtcgca
     2341 ccgttcctgt tcacggcggt ccgctacggg ttcgcgatcg cgtggaagat caccgcgctg
     2401 acggagctct tcggtcagac agacggggtc gggatccaga tccgcgtcga gttcctgttc
     2461 ttcgacatcg ccggcgtgct ggcgtgggcg ttcttcctcg tcggcttcgc gctcgtgatg
     2521 gaacggctcg tgctccagcg gctcgagcgc cggttcttcc gctggcgccc gcaggcgttc
     2581 gcgtaggagg gcgcatggga gggacgatgg agcagaccgt cggcgccagg aaagggaccg
     2641 tccccgcgag acgttcgtgg ttcgagcgga cgttcctcac gccacgcgct gcgcggatca
     2701 ccgcaccggt cctgttcctc gtcgcgtggc agctgatcgc tcccctcatc ccttccgtga
     2761 tcctccccat gcccgcggag gtcgtcgagg cgatgtggga gcagctccgg gacggcacct
     2821 tggtggaggc gttctcggtg agtctcctcc gcctcgcgat cgggttcgcg ctggcgctcg
     2881 cggttggctc gatcgtcggc ctggcgatgg ggctctcccc acgtatcgag gcgatgctgc
     2941 acgacttcgt ggtcgtcggt ctcacgttcc cctacctgat ctgggggctg cttgtcgcga
     3001 tgtggttcgg ctacggatcg atcggccccg tgctcgtggt cttcatcgcc gggctgccct
     3061 acgtgatcct gaacatgtcc gaaggagtgc aagacgtgtc gaaggagctc cgcgacatgg
     3121 cggctgcata cgaggtccct cgcgaccggg tgatgcggca cctgatcctg ccgtcgctca
     3181 gcccgttctt cttcgcctcg ctccgctacg ggctggcgaa tggctggaag ggcctgatcc
     3241 tcgccgaggt gttcgccgcg acgtccggcg cggggtggca catcaccagc atgcgcgagt
     3301 tcggcgactt cgccggcgtc ttcggattgg cgatctactt ctcgctgttc tcgatcgtgg
     3361 ttgagcggct cgtcttcgga cgcctgtcac ggcgcgtgtt ccgctggcga cccacgctgg
     3421 cgcaggagat gagcgaaccc ccggagctga ccatatgaca ccgaacgaca ggaggcctga
     3481 gccgtggcga ccgtcaccgt gaccgatctg cggaaagtat tcctcggcga gggccgggac
     3541 gtcgtcgccc tcgacggggt ggggttcgac gcatccggcc acacgttcgt gagcatcgtc
     3601 gggccgtcgg ggtgtgggaa gtcgaccctc ctgaacatcc tcgcgggcgt cgagacgccg
     3661 acctcaggcc gtgccgatat cacggagaat ggcacgcggg ctcgcgtggg gtacgtcttc
     3721 caggccccgc gactgctccc ctggcgctcg gtgatggaca acatgctgtt cgtgcagccg
     3781 gagcaatccg aggaggtccg cgatcgcgcc gcgcactacc tcgacatggt cggactgagc
     3841 gagcacctca aggcgtggcc gtcccagctg tcgggtggca tgcagcagcg ggtgggcatc
     3901 gcccgggcgt tctcgatcga gcccgacgcg ctgctgatgg acgagccatt cagccacctc
     3961 gacgcgatca ccgcccgtgg gctgcggcgg gagctgcact cgatgtggaa ggagacgaag
     4021 aagaccgtgc tgttcgtgac ccacgacgtg ggcgaggcgg tggagctctc cgaccgcatc
     4081 gtgatcctgg cgaagggcgg caagctgcgt caggacctga ccgtcgacct gccttacccg
     4141 cgcgacccgg cggacgacaa ggtcgcccta
//
LOCUS       DASUVE010000143         3371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1388208, whole genome shotgun sequence.
ACCESSION   DASUVE010000143 DASUVE010000000
VERSION     DASUVE010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388208"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VF036_03000"
     CDS             complement(<1..525)
                     /locus_tag="VF036_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX6264228.1"
                     /translation="MPRPGFVLEVDERTPPLLVHEGEGYRMQKFPLGTRVVYPPDPLP
                     GIRDVNAAIRHALLHPLGDSEPLPELLKTGMKLTIAVDDISIPLPPMTLPDIRQRILE
                     QVIELAARKGVEDVQIVVATALHRRMTADEIRRMVGERVFRSFWPKDLFNFDGEDKDA
                     LTEVGTTDEHEVVEI"
     gene            complement(557..1765)
                     /locus_tag="VF036_03005"
     CDS             complement(557..1765)
                     /locus_tag="VF036_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6264229.1"
                     /translation="MALALEYYRSAPKYLGARTVGSKVPGLVTAGLAPLRLVTQKDPE
                     PVREGWARVKPHLSGICGSDLSTISGRSSFYFSPLVSLPFVPGHEVVGELLDDCGDLK
                     AGERVVISSVLGCAARGVDPPCEYCAAGHVGRCDRVTVGHLKPGLQTGYCSETGGGWS
                     RLMLAHRSQLWPVPDGMGDETAVLIEPLACAIHAAFRARIPAGGTVHVVGAGTVGILT
                     LIAIKAFTKADHVIVSAKHQRQRAAASMAGADDVVRPEHAVKAVRRTDHAVKLTPERG
                     MEFLLGGVDVAIECTGSSSALDLALRTTKAGGRIVVSGIPGGGADLTPLWFRELELAG
                     AYTSGVEDLDDGVTRPTFEMAIELASNLPILEQLVGATYPLDRWRDALDHAMAAGTLG
                     TFKVAFAPQT"
     gene            complement(1771..>3371)
                     /locus_tag="VF036_03010"
     CDS             complement(1771..>3371)
                     /locus_tag="VF036_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX6264230.1"
                     /translation="DWVSTRLTEYGRMRARSLGWPDVYTFTKAMGERAVEELALAHEA
                     PLSIVRPSIIESAYTHPFPGWIDGFKMADPIIRAYGLGQIPEFPGIPEGIVDLIPVDY
                     VVNAILAVAANPPPEGEAAHYNVSSGSRNPLRFYELYEWVREYFEAHPLPERGRGEHK
                     VPTWTFPGNVRVESLLRRAERLTDVAEKVVTHLPKSKAMREAVRRVDRDKARVDFVKR
                     YSDLYGMYTETEVVYTDERVVALWASLSDEDRERFPFDAAVIDWRYYLQDVHSPAVTK
                     SLRELSKRDRERPVVRVQKREEPVLAVFDMEGTIITSNVVESYVWTRMADLPADEWPA
                     ELASVFGRIPGYLQTDRRDRGDFLRTFFRRYEGASVEGLERLVDRHVGEFMLQKASPG
                     AIRRIREHRAAGHRTLLLTAAANAFLRPLQPLFDVVIGADLQVDAEGRYTGFMSTPPL
                     VGEARAAWLRRYAAGEGVDLKTSYAYADSYSDLPLLRAVGNPVAVSPDSHLYRYARRR
                     RWPIEEWAMSKGMPRVRFPKPARR"
BASE COUNT          516 a         1207 c         1140 g          508 t
ORIGIN      
        1 gatctcgacg acctcgtgct cgtccgtggt accgacctcc gtcagcgcgt ccttgtcctc
       61 gccgtcgaag ttgaagaggt ccttcggcca gaacgaccgg aacacccgct ctcccaccat
      121 gcgccggatc tcgtcggcgg tcatccggcg atggagggcg gtcgccacga cgatctggac
      181 gtcctcgacg cccttgcgtg ccgcgagctc gatcacctgc tcgaggatcc gctgccggat
      241 atcgggaagg gtcatcggcg ggagcgggat cgagatgtcg tccaccgcga tcgtgagctt
      301 catccccgtc ttgagaagct cggggagcgg ctccgagtcc ccgagcggat gcagcagcgc
      361 gtgccgaatc gcggcgttca cgtccctgat gcccggaagc gggtcggggg ggtacaccac
      421 gcgcgtcccc aaggggaact tctgcatgcg gtagccctcc ccttcgtgga ccaggagcgg
      481 tggcgtccgc tcgtccacct cgagcacgaa tccgggtctg ggcatcggtc gtcgtctcct
      541 gtcgtcgttg tcttccctac gtctgcggcg cgaacgccac cttgaacgtg ccgagggtgc
      601 cggccgccat cgcatggtcg agcgcgtccc gccaccgatc caacggatac gtggcgccga
      661 cgagctgctc caggatcggc aggttcgagg cgagctcgat cgccatctcg aacgtcggtc
      721 gggtgacccc atcgtcgagg tcttcgacgc cggacgtgta ggccccggcg agctccagct
      781 cgcggaacca caggggcgtg aggtcggcgc cgccgccggg gatgccggag acgacgatgc
      841 gaccacccgc cttcgtggtg cggagcgcga gatcgagcgc gctgctcgat cccgtgcact
      901 cgatcgccac gtcgacgccg ccgagcagga actccatgcc gcgctccggc gtgagcttca
      961 ccgcgtgatc cgtgcgacgc acggccttca cggcgtgctc cggccggacg acgtcgtcgg
     1021 cgccggccat cgatgcggcg gcacgctgac gttggtgctt cgccgacacg atcacgtggt
     1081 ccgccttcgt gaacgccttg atcgcgatca acgtcaggat cccgaccgtg ccggcgccga
     1141 cgacgtggac cgtcccgccc gccgggatcc tcgcgcggaa cgccgcgtgg atcgcgcacg
     1201 cgagcggctc gatcagcacc gcggtctcgt cccccatccc gtcgggcacc ggccagagct
     1261 gtgatcggtg ggcgagcatc agacggctcc accccccacc ggtctccgag cagtacccgg
     1321 tctggagccc cggcttcaga tggccgacgg tgacgcggtc gcagcggccc acatggcctg
     1381 ccgcgcaata ctcacacggt gggtccactc cccgagccgc gcacccgagg acgctcgaga
     1441 tcacgacgcg ctcgccggcc ttcaggtcgc cgcagtcgtc caggagctca ccgacgacct
     1501 cgtgtcccgg gacgaacggg agggacacga gcggcgagaa gtagaacgag gagcgcccgg
     1561 agatcgtgga gaggtcgctg ccgcagatgc cggacagatg cggcttcacg cgcgcccatc
     1621 cttcgcggac cggctcggga tccttctggg tcacgagccg gagcggcgcg agtccggccg
     1681 tcacgaggcc gggcaccttc gagccgaccg tgcgcgcgcc gaggtacttc ggtgcggagc
     1741 ggtagtactc gagcgccaat gccaccgtcc ctacctcctc gccggcttcg ggaatcggac
     1801 gcgtggcatg cccttcgaca tcgcccactc ctcgatcggc cacctgcgcc ggcgcgcgta
     1861 ccggtacagg tgcgagtcgg gcgacacggc gaccgggttc cccaccgcgc ggagcagggg
     1921 caggtcggag taggagtcgg cgtacgcgta ggaggtcttc aggtcgacac cctcaccggc
     1981 cgcgtaccgg cggagccagg ccgcccgggc ctcgccgacc agcggaggcg tggacatgaa
     2041 ccccgtgtag cgaccctcgg cgtccacctg gagatcggcc ccgatcacca catcgaacag
     2101 cggctgcagg ggccggagga acgcgttcgc ggcggcggtg agcaggagcg tccggtgccc
     2161 ggcggctcgg tgctcccgga tccggcggat cgcaccggga ctcgccttct ggagcatgaa
     2221 ctccccaacg tgccggtcga cgagccgctc gagaccctcg accgacgcgc cctcgtaccg
     2281 ccggaagaac gtccgcagga agtcgccgcg gtcgcgccgg tcggtctgca ggtacccggg
     2341 gatccgcccg aacaccgacg cgagctcggc cggccactcg tcggcgggca ggtcggccat
     2401 ccgcgtccac acgtaggact cgacgacgtt cgaggtgatg atcgtcccct ccatgtcgaa
     2461 cacggctagg accggctcct cgcgcttctg cacccggacg accggccgct cgcggtcgcg
     2521 cttcgacagc tcgcgcaggc tcttcgtgac cgccggcgag tgcacgtcct ggaggtagta
     2581 gcgccagtcg atcacagccg cgtcgaacgg gaaccgctcg cggtcctcgt cgctcagcga
     2641 cgcccacagg gcgacgacgc gctcgtccgt gtacacgacc tcggtctccg tgtacatgcc
     2701 gtacaggtcg gagtagcgct tcacgaagtc gacgcgcgcc ttgtcgcggt cgacccggcg
     2761 gacggcctcg cgcatcgcct tggacttcgg gaggtgcgtc acgaccttct cggcgacatc
     2821 ggtcaagcgc tctgcccgac gcagcaacga ctccaccctc acgttccccg ggaacgtcca
     2881 ggtcggaacc ttgtgctccc cccgtccgcg ctccggcaac ggatgcgcct cgaagtactc
     2941 ccggacccat tcgtagagct cgtagaaccg cagcgggttc cgggagccgg aggagacgtt
     3001 gtagtgggcg gcctcgccct cgggcggcgg gttggccgcg accgcgagga tcgcgttcac
     3061 cacgtagtcc accgggatca ggtcgacgat gccttcgggg atccccggga actccgggat
     3121 ctgtccgagc ccgtacgcgc ggatgatcgg atcggccatc ttgaacccgt cgatccaccc
     3181 agggaacggg tgcgtgtacg ccgactcgat gatcgaggga cgcacgatcg acagcggcgc
     3241 ctcgtgcgcg agcgcgagct cctcgaccgc acgctcaccc atcgccttcg tgaacgtgta
     3301 gacgtcgggc caaccgaggg agcgagcgcg catgcggccg tactcggtga gtcgggtgct
     3361 gacccagtcc t
//
LOCUS       DASUVE010000144         4390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1012046, whole genome shotgun sequence.
ACCESSION   DASUVE010000144 DASUVE010000000
VERSION     DASUVE010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012046"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VF036_03015"
     CDS             <1..1159
                     /locus_tag="VF036_03015"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6264231.1"
                     /translation="GYDGGLHLIGAEDEPPYERPPLSKELLRGEATLEDALVRPRSWY
                     AEHDVDARFGTRAAQIDVHRGEVICTGGDRIAYDRLLVATGSRNRSLEVPGAGLPGVF
                     DLRTYGDAVGIREAATSGARVVCVGMGFIGAEVAASLRSVGCDVTVVEIFETTLYRIL
                     GPEIGRVLEAIHRDHGVTMHFNDTVERFEGDGRLERVVTEGGKRIDADVAVVGVGTEA
                     AVEIMAGTGLDQGGGIPVGPTLETSVPGVFAIGDVARHDHPVFGPIRVEHYDNAIKMG
                     EHAAKAMLGSADVFDDPHWFWSDQYDSKVEMAGFAPTWDRMIVRGSLEDRSFCAFLLD
                     DGGVLRSTVSLDRKRDVRRSFGLIKAQLVPDPALLADPDVDLRKLVEEEGE"
     gene            1160..1357
                     /locus_tag="VF036_03020"
     CDS             1160..1357
                     /locus_tag="VF036_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264232.1"
                     /translation="MFGSPVVTHPVCNLDLHQRIRDARRDAVARSIRGRLRHRRQARV
                     VDVDGEVFRPTGEDVGQAFRF"
     gene            complement(1383..2099)
                     /locus_tag="VF036_03025"
     CDS             complement(1383..2099)
                     /locus_tag="VF036_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6264233.1"
                     /translation="MPEPALVEGIDVVRRYPGAQGVIAVRGASVTVRPRDFVALMGPS
                     GSGKTTFLGVLAGLDPPDDGEVKWKGRSMSSMSGDERRELRQHGIGVVFQSFGLLPAL
                     SAVENVELPLRVNGVDLTEARERAEGWLQRLGLETRMHNRTYELSAGQQQRVAVARAL
                     IPEPEIVMADEPIAEVDTENADIILEALWDVIRRDGAVLAATHNPEALRYANRVVLFR
                     DGVVEQEGTPQEVAGHLTVD"
     gene            complement(2092..2961)
                     /locus_tag="VF036_03030"
     CDS             complement(2092..2961)
                     /locus_tag="VF036_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017250986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6264234.1"
                     /translation="MTENVVSAERYRPEGADALVRLRHAYKVYRVGDMGVVALAGVDL
                     DVMPGEFLAVVGPSGAGKSTILNLVGGLDRASAGIVEVEGRDLGLLEDQELTEYRAER
                     IGFVWQGTARNLVPYLSVRENIRLPATVRRTQVAKKHDVDGLLELVGLHDRAKHTPGM
                     LSGGEQQRVAIAAALSNTPSILLADEPTAELDSESAARVLDAFRAVNREFHVTIIMVT
                     HDLVAARRADRRIRVRDGRVLHEGVPVDPVSEDGRVRLPDEAIAAIGGVDLEAEVSAD
                     EVRLRRRSETGHA"
     gene            complement(2958..>4390)
                     /locus_tag="VF036_03035"
     CDS             complement(2958..>4390)
                     /locus_tag="VF036_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6264235.1"
                     /translation="NHEDAAHAQVGADWRIDVTPPDDILSVIERMPPLTTAVVRTEPR
                     LESGTFNLPPIALGSDPERHAAAGWWREDFSETPLPEIMRRLQTPPFGMTLPAPTSTL
                     TLEIESSEDVRNLQIAATGVNADGFVHTAPLQPVEPGLTRYELRLDAAVRLFSITIHR
                     TSLDSPDEVTFEFRSIEADGEAISLDGWEPLTWRGSAGSVEPEGAGVRYEMESGASDV
                     IGGIVPGSDPLPVLVSPGIALADGPIFPATLGGQELELDPVAGALQFPSMIPNAPFIV
                     LSGPALLERAAAVPEPGLVLNEVWAEGDRDPIPLLRADGLISGAVARTAPIEGFLAQL
                     PQSLALGMNFVAAAGGAGLVVVGVAAGLYFAQRRRDYEFAALRAMGTAPGQIVRTLAL
                     EQVLLLGFAVVAGLGLGYLLLRLMMPYVGPSLSVAYPPPVFLMDWTSLGIALAAILGA
                     TAIALALSSRALLRASVTGVLRGEAE"
BASE COUNT          661 a         1561 c         1536 g          632 t
ORIGIN      
        1 aggctacgac ggcggcctcc acctgatcgg ggccgaggac gagccgccct acgagcgtcc
       61 gccactgtcg aaggagctcc tgcggggcga ggcgaccctc gaggacgcgc tcgtgcgccc
      121 caggtcctgg tacgcggagc acgacgtcga cgcccggttc gggacgcgag cagcacagat
      181 cgacgtccac cggggcgagg tgatctgcac tggaggcgac cgcatcgcct acgaccggct
      241 cctggtcgcg acgggctccc ggaaccgcag cctcgaggtg ccgggcgcgg gtctccccgg
      301 tgtcttcgat ctccgcacct acggcgacgc cgtggggatc cgggaggcgg ccacctcggg
      361 cgctcgagtc gtgtgtgtcg gcatggggtt catcggcgcc gaggtcgctg cgtccctgcg
      421 atcggtgggg tgcgacgtaa cggtcgtcga gatcttcgag accaccctgt atcggatcct
      481 cgggcccgag atcggtcgcg tgctcgaggc gatccatcgc gatcacgggg tgacgatgca
      541 cttcaacgac acggtcgagc ggttcgaggg agacggaagg ctcgaacgcg tcgttaccga
      601 gggcgggaag cggatcgacg ccgacgtcgc ggtggtgggc gtcgggacag aggccgccgt
      661 ggagatcatg gccgggaccg gcctcgatca aggcggcggg atcccggtgg ggcccacgct
      721 cgagacgagt gtgccgggcg tgttcgcgat cggcgacgtc gcccgccacg accatccggt
      781 cttcggcccg atccgcgtcg agcactacga caacgcgatc aagatggggg agcatgccgc
      841 gaaggcgatg ctcggctcgg ccgacgtctt cgacgaccct cactggttct ggtccgacca
      901 gtacgactcc aaggtcgaga tggcgggctt cgccccgacg tgggaccgta tgatcgtccg
      961 cggttccctg gaagaccgtt ccttctgcgc cttcctgttg gatgatggag gggtgctgcg
     1021 ttccaccgtg agcctcgatc ggaagcgcga cgtaaggagg tccttcggat tgatcaaggc
     1081 gcagctcgtc ccggatccgg cgttgctcgc cgaccccgac gtggacctgc ggaagctcgt
     1141 cgaagaggaa ggggagtagg tgttcggctc gcccgtcgtc acgcaccccg tgtgcaacct
     1201 cgacctccat cagcggatcc gcgacgcacg gcgcgatgcc gtggcccggt cgatccgagg
     1261 caggctgcgg catcgccgac aggctcgcgt ggtcgacgtc gacggcgagg tcttccgtcc
     1321 aacgggcgag gacgtcggcc aagccttccg gttctgacgc gcgcgggaga ggaacccccg
     1381 agctagtcga ccgtgaggtg tcctgcgacc tcctggggcg tgccttcctg ctccacgaca
     1441 ccgtcgcgga agagcaccac gcggttcgcg tagcggaggg cttcggggtt gtgcgtcgcg
     1501 gccagcaccg cgccgtcgcg ccggatcacg tcccagagcg cctcgaggat gatgtccgcg
     1561 ttctccgtgt cgacctcggc gatcggctcg tcggccatca cgatctcggg ctccgggatc
     1621 agcgcgcggg ccaccgccac gcgctgctgc tgcccggccg acagctcgta cgtgcggttg
     1681 tgcatccgcg tctcgaggcc gagtcgctgg agccatccct ccgcgcgctc tcgcgcctcc
     1741 gtcagatcca caccgttcac gcggagcggc agctccacgt tctcgaccgc ggagagcgcc
     1801 ggcagcaacc cgaagctctg gaacacgacg ccgatgccgt gctgtcggag ctcgcggcgc
     1861 tcgtcgcccg acatcgacga catcgaccgg cccttccact tcacctcgcc gtcatcgggg
     1921 ggatcgaggc ccgcgagcac tcccaggaac gtcgtcttgc ccgagccgct cggtcccatc
     1981 agcgccacga agtcccgcgg ccggaccgtg actgaggcac ctcgcaccgc gatgaccccc
     2041 tgcgcgcccg ggtaccggcg aacgacatcg atcccctcga cgagcgcggg ctcaggcatg
     2101 gccggtctca ctccttcgcc gcagccgcac ctcgtcggcg gagacctcgg cttcgaggtc
     2161 gaccccgccg atcgcggcga tcgcctcgtc gggcagccgc acacgaccgt cctcggacac
     2221 ggggtcgacc ggcacccctt cgtgcaggac gcgcccgtcg cgcacccgga tccggcggtc
     2281 ggcgcgccgg gcggccacga ggtcgtgggt gaccatgatg atcgtgacgt ggaactcgcg
     2341 gttcacggcg cggaacgcgt cgaggacccg agccgcggac tcggaatcca gttccgccgt
     2401 cggctcgtcg gccagcagga tcgagggggt gttcgacagc gcggccgcga tcgccacccg
     2461 ttgctgctcg ccgccggaga gcatgcccgg ggtgtgcttc gcccggtcgt ggagcccgac
     2521 cagctcgagc aatccgtcga cgtcgtgctt cttcgccacc tgcgtccggc gcacggtggc
     2581 gggcaaccgg atgttctccc gcaccgagag gtacggcacc aggttgcgcg ccgtgccctg
     2641 ccacacgaag ccgatccgtt cggcgcggta ctcggtgagc tcctgatcct cgaggagccc
     2701 cagatcgcgc ccttccacct cgacgatccc ggccgacgcc cggtccagcc cgcccaccag
     2761 gttcaggatc gtggacttcc cagcgccgct cggaccgacg acggcgagga actcgcccgg
     2821 catcacgtcg aggtcgacgc ccgcgagcgc cacgaccccc atgtcgccca cgcggtagac
     2881 cttgtacgcg tggcggagac ggacgagcgc gtccgcacct tccggccggt agcgctccgc
     2941 actgacgacg ttctccgtca ctcggcttcc cctcggagta cgccggtcac cgacgcccgg
     3001 aggagggcgc gtgaggacag cgccagcgcg atcgcggtcg cgccgaggat cgcggcgagc
     3061 gcgatcccga gcgacgtcca gtccatcagg aacacaggcg ggggatacgc gaccgagaga
     3121 ctcggcccga cgtacggcat catcaggcgg agcaacaggt agcccaggcc gagtccggcg
     3181 acgaccgcga accccaggag cagcacctgc tcgagggcga gcgtccggac gatctgcccg
     3241 ggggcggtcc ccatcgcccg gagcgcggcg aactcgtagt cacggcggcg ctgtgcgaag
     3301 tacagcccgg ccgcgacccc caccaccacg agtccggcgc ctcccgccgc cgccacgaag
     3361 ttcatcccca gggcgaggct ctgtgggagc tgcgcgagga acccctcgat cggcgccgtc
     3421 cgggccaccg ctccggagat cagaccgtcc gcgcggagca gcgggatcgg gtcgcgatcc
     3481 ccctcggccc atacctcgtt cagaacgagg cccggctccg ggaccgcggc agctcgttcg
     3541 aggagggccg ggccggacag cacgatgaac ggggcgttcg ggatcatcga ggggaactgc
     3601 agcgctccgg ccacggggtc cagctccaat tcctgtccgc cgagggtcgc ggggaagatc
     3661 ggaccgtcgg ccaaggcgat gccgggggag accaagaccg gcaggggatc ggagccaggg
     3721 acgatgcccc cgatcacgtc ggaggcgccc gattccatct cgtagcgaac cccggcgccc
     3781 tcgggttcga cggatcccgc gctcccgcgc cacgtgagcg gctcccatcc gtccagggag
     3841 atcgcctctc cgtcggcctc gatcgaccgg aactcgaacg tcacctcgtc cggcgaatcc
     3901 agcgacgtcc gatggatcgt gatcgagaac agacggacgg cggcatccag gcgcagctcg
     3961 tagcgggtga gcccgggctc caccggttgg agcggagcgg tgtgcacgaa accgtccgcg
     4021 ttcaccccgg tcgccgcgat ctggaggttc cggacgtcct cgctcgactc gatctccagc
     4081 gtgagcgtgg aggtcggcgc cggcaaggtc atgccgaacg gaggcgtctg gagccggcgc
     4141 atgatctccg gcaggggcgt ctcggagaag tcctcccgcc accagcccgc cgccgcatgc
     4201 cgctcggggt cgctcccgag cgcgatcggg gggaggttga acgtgccgct ctcgaggcgt
     4261 ggctccgtgc gcacgacggc ggtggtgagc gggggcatcc tctcgatcac ggacaggatg
     4321 tcgtcggggg gcgtcacgtc gatccgccaa tccgcgccca cctgggcgtg cgcggcgtcc
     4381 tcgtggttgc
//
LOCUS       DASUVE010000145         4412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1577802, whole genome shotgun sequence.
ACCESSION   DASUVE010000145 DASUVE010000000
VERSION     DASUVE010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577802"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VF036_03040"
     CDS             <1..522
                     /locus_tag="VF036_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6264236.1"
                     /translation="YTLGILPFMLPPIISALGMQVFWLSTGHVGKIENVIIAHAIFFS
                     TLPLVTISLAMETMDQALPEAARTLGADQRTTFRTITLPLLIPSMVTGYAAAFVLSLN
                     EYIISFLVAGFTVETLPVKIVNGLRYGFTPTIAAVAVVFIVIAATTFTLFAIFGNLMR
                     FLGADPAILEKNR"
     gene            complement(519..1496)
                     /gene="speB"
                     /locus_tag="VF036_03045"
     CDS             complement(519..1496)
                     /gene="speB"
                     /locus_tag="VF036_03045"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX6264237.1"
                     /translation="MADDPGAGRPQEERSPVGPIDPLVVPRFAGPATFARLPRREDVD
                     AGDVAILGIPFDSGVTYRPGARFGPQAVRSASRLLRSYHPALDVKPFAAQQVVDAGDV
                     ACNPFDIAEAITQIEAGAEEALDSASALLSIGGDHTVALPLLRVMHRRHGPIALVHFD
                     AHLDTWDTYFGAAYTHGTPFRRAWEEGLLLEDKATHIGIRGPLFSPDDLVDDARFGFE
                     IVTAMDVLDEGRAAVADRVRSRLADAPVYVSIDIDALDPAFAPGTGTPEAGGLTSREL
                     LGILRGFAGIRLVGADIVEVAPPYDHAEMTAVAASHAAYELLALFSSKA"
     gene            complement(1580..2017)
                     /locus_tag="VF036_03050"
     CDS             complement(1580..2017)
                     /locus_tag="VF036_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264238.1"
                     /translation="MQIVRKVMAAVALLLVGGLVIGATMAFAGDSPSPSASVASTASA
                     SSASETPSASETPAIGDDGTADQGPGDVPVGTGDLTDGGLVSGDDDGTLDQGPGDFSF
                     GDDDGDDRDDDADDEQGDDDNSGPSENSGPGSVDDDSGHGSDD"
     gene            complement(2120..2464)
                     /locus_tag="VF036_03055"
     CDS             complement(2120..2464)
                     /locus_tag="VF036_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264239.1"
                     /translation="MKRVVAMIVLGIGGLAAAIALTVGALALAGDVGTVVQPTLSGSG
                     SASRSPSASAEEEGRSETPSPSADDHGGGSDDPAGSDDSGGNSGPGSDNSGSGSGNSG
                     SGSDDSGSHDDD"
     gene            complement(2489..3892)
                     /locus_tag="VF036_03060"
     CDS             complement(2489..3892)
                     /locus_tag="VF036_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6264240.1"
                     /translation="MKVRTRLAASFAYVLLVVIVALTVPLGIVLRDRARSELEALTLA
                     NAQTIAAVLTRDQLEDDLAERRALVRNAARYAGDVGGRVVVLDDAGVAIADTAEEDLG
                     EDFATPGRPEVASALASRPLAEVRRSEEEEGDIAVAAAPIIDEGELVGVVRISRDVAT
                     VQANVGRATAAIVAVALGGLIAGLVLVLAIARSLARPLSRLASTARRLGDGDLSARAG
                     SLEGGDEVHELAGSFDEMASRMERTVRAQREFVANASHQLRTPLTGIKLRLEAGIAES
                     DDEEVRTQLRAADKEVDRLSQIIERLLTMASQIEEGTVPHADLGDAVERAVHRWRERA
                     VRGSAELSNEIDGAELAVVADPADVDQVLDVLIDNALTHAAGPIRVIASAGPGRAIVS
                     VVDRGPGIPEDEVARVTERFFRGRAAATGGSGLGLAIARELAERWGGGLDLRSEDFST
                     RVDVWFPVPPDGALVGP"
     gene            complement(3920..>4412)
                     /locus_tag="VF036_03065"
     CDS             complement(3920..>4412)
                     /locus_tag="VF036_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6264241.1"
                     /translation="DVRAHSDVPIVMLTARGEEVDRIVGLELGADDYVVKPFSAGELV
                     ARIRAIQRRGRAAGSRRAPITIGDLVLDPASRSVTKDGAPIELAAKEFDLLRMLMSRA
                     GEVVGREEIMDEVWDPHWFGPTKTLDVHISWLRKKIEDEPSAPRYITTVRGVGFRFAA
                     PSP"
BASE COUNT          631 a         1607 c         1520 g          654 t
ORIGIN      
        1 tacacgctgg ggatccttcc gttcatgctg ccgccgatca tcagcgcgct cgggatgcag
       61 gtgttctggc tctcgacggg tcacgtcggc aagatcgaga acgtgatcat cgcgcacgcg
      121 atcttcttct cgacgcttcc gctcgtcacg atctcgctcg cgatggagac gatggaccag
      181 gcgctcccgg aggccgcacg gacgctcggc gcggatcagc ggacgacgtt ccggacgatc
      241 accctcccgc tgctgatccc gtcgatggtc accgggtacg cggcggcctt cgtcctcagc
      301 ctgaacgagt acatcatctc gttcctcgtc gccggattca cggtcgagac gctcccggtg
      361 aagatcgtga acgggctccg ctacgggttc acgccgacga tcgcggcggt cgcggtggtc
      421 ttcatcgtga tcgccgccac gacgttcacc ctgttcgcga tcttcggcaa cctgatgcgg
      481 ttcctcggcg ccgacccggc gatcctggag aagaaccgct aggccttcga gctgaacagt
      541 gcgagcagct cgtaggcggc gtgtgaggcg gccaccgccg tcatctccgc gtgatcgtac
      601 ggcggggcga cctcgacgat gtccgcgccc acgagccgga tgcccgcgaa gccgcggagg
      661 atcccgagga gctcgcggct cgtgagacca ccggcttccg gtgttcctgt tcccggcgcg
      721 aaggcgggat cgagcgcgtc gatgtcgatc gagacgtaga ccggcgcatc ggcgagccgg
      781 ctgcgaaccc tgtccgcgac cgccgcgcgg ccctcgtcca ggacgtccat cgcggtcacg
      841 atctcgaacc cgaaccgggc gtcgtccacg aggtcgtccg gtgagaagag cggcccccgg
      901 atcccgatat gggtggcctt gtcctcgagc aggaggccct cctcccaggc gcggcggaac
      961 ggtgttccgt gcgtgtacgc ggccccgaag taggtgtccc aggtatccag gtgcgcgtcg
     1021 aagtgcacga gcgcgatggg gccgtgacgg cggtgcatga cccgcaggag cgggagtgcg
     1081 accgtgtgat cgccgccgat cgacagcagc gccgaggcgg agtccaacgc ctcctcggcg
     1141 cccgcctcga tctgcgtgat cgcctcggcg atatcgaagg gattgcacgc gacgtcgccg
     1201 gcgtcgacga cctgctgcgc ggcgaacggc ttcacgtcca gggcgggatg gtaggaccgg
     1261 agcaaccggg acgcgctccg cacggcctgc ggaccgaaac gggcccccgg gcggtaggtc
     1321 acgccgctgt cgaaggggat cccgaggatg gccacgtcgc cggcgtcgac gtcctcgcgt
     1381 cgaggcaggc gagcgaacgt cgcggggccg gcgaatcggg ggacgacgag cgggtcgatc
     1441 gggccgaccg gagagcgctc ctcctgcggt cgcccggcgc ccggatcatc ggccatcgcg
     1501 agcagatgct agcggccgac gcggaagggc ggcggcgccg tcggcgccgt cgccctcggg
     1561 cgtcgaggac atccgtccct cagtcgtcgc tcccgtgccc ggagtcgtcg tcgacgctcc
     1621 cgggaccgct gttctcgctc ggaccggagt tgtcgtcgtc tccctgctcg tcgtcggcat
     1681 cgtcgtcacg gtcatcgccg tcgtcgtcac cgaacgagaa gtcgccgggg ccctggtcga
     1741 gcgtcccgtc gtcgtctccg gagaccagcc ctccgtccgt gagatctccg gtcccgaccg
     1801 ggacgtcgcc ggggccctgg tccgccgtgc cgtcgtctcc gatcgccggg gtctcgctgg
     1861 ccgacggtgt ctcgctggcc gacgaggcgc tcgccgtcga cgcgaccgag gcgctcggac
     1921 tcggcgagtc tcccgcgaag gccatggtgg ctccgatcac gagtccgccc accagcagca
     1981 acgccaccgc cgccatcacc ttccgcacga tctgcatgtc ttcctcctct gtcgtcgatc
     2041 tcgtcgagat cgcctacgac caaggtaggg agcggcgggt taccggctgc ccagaggcag
     2101 gtaaaggcgg cggtaagcct cagtcgtcgt cgtgcgaacc ggagtcgtcc gagccggagc
     2161 cggagttgcc ggagcccgac ccggagttgt ccgagcccgg cccggagttg ccgcccgagt
     2221 cgtcgctgcc ggcggggtca tcggaccctc cgccgtggtc gtcggccgac ggggacggcg
     2281 tctcggagcg gccttcttcc tcggccgacg cgcttgggga tcgggaagcg gagccggatc
     2341 ccgagagggt cggctgcacc accgtgccca cgtccccggc cagtgccagc gcgccgacgg
     2401 tcagcgcgat cgccgcggcg aggccgccga tgccgagcac gatcatggcg acgacgcgtt
     2461 tcatgcctcc accgtagcgg cccggtcgtt aaggaccgac taaggcgccg tcgggaggca
     2521 cggggaacca cacgtccaca cgggtgctga agtcctcgga gcgcaggtcg aggccaccgc
     2581 cccagcgttc ggcgagctca cgggcgatcg ccaaccccaa ccccgacccg cccgtggctg
     2641 cggcgcgacc gcggaagaag cgctccgtca cccgcgcgac ctcgtcctcg gggatccccg
     2701 gtccacggtc cacgacggag acgatcgctc gtcctggtcc ggcggaggcg atgacccgga
     2761 tcgggccggc ggcgtgcgtg agcgcgttgt cgatcaggac gtcgagcacc tggtccacat
     2821 cggcgggatc ggcgaccacc gcgagctcgg cgccgtcgat ctcgttcgac agctcggcgg
     2881 acccccgcac cgcccgctca cgccatcgat gtaccgcccg ctcgacggcg tcccccaggt
     2941 cggcgtgggg aaccgtcccc tcctcgatct ggctcgccat cgtcagcagc cgttcgatga
     3001 tctgcgagag gcggtcgacc tccttgtcgg cggcccggag ctgggtccga acctcctcgt
     3061 cgtcggactc cgcgatcccg gcctcgaggc gcagtttgat ccccgtgagc ggcgtgcgca
     3121 gctgatggga cgcgttcgcg acgaactccc gctgcgcgcg gaccgtccgt tccatccggg
     3181 aggccatctc gtcgaacgat cccgcgagct cgtggacctc gtcccctccc tccagggacc
     3241 cggcgcgggc cgagagatcg ccgtccccga gacgccgagc cgtcgaagcc agccgggaca
     3301 gcggccgcgc gagcgaccgc gcgatcgcga ggaccagcac gagccccgcg atgagcccac
     3361 cgagcgccac cgccacgatc gccgcggtgg ctctcccgac gttcgcctgc acggtcgcca
     3421 cgtcccgtga gatccgcacg acgccgacca actcgccttc gtcgatgatc ggggcggccg
     3481 cgaccgcgat gtccccttcc tcctcctcgc tccgccggac ctccgcgagc ggccgcgagg
     3541 cgagggcgct ggccacctcc ggtcgccccg gcgtggcgaa gtcctcgccg agatcctcct
     3601 cggcggtgtc ggcgatcgcg acgccggcgt cgtcgagcac cacgacgcgg ccgcctacgt
     3661 ctcccgcgta ccgcgctgcg ttccgaacga gcgcccggcg ctcggcgaga tcgtcctcga
     3721 gctggtcgcg cgtgaggacg gcggcgatcg tctgggcgtt cgcgagggtc agcgcctcga
     3781 gctcggaacg ggcgcgatcg cggagcacga tgccgagcgg gacggtcagc gccacgatca
     3841 cgacgagcag gacataggcg aacgaggcgg ccagccgtgt ccggaccttc atctgcgtgc
     3901 gcccacctgc atcgcccggt cagggagagg gcgcggcgaa ccggaacccg acgccgcgga
     3961 ccgtggtgat gtaccggggc gccgacggct cgtcctcgat cttcttccgc agccacgaga
     4021 tatgcacgtc gagggtcttc gtgggtccga accagtgcgg atcccagacc tcgtccatga
     4081 tctcctcccg cccgaccacc tccccggccc gagacatcag catcctcagg aggtcgaatt
     4141 ccttcgcggc gagctcgatc ggagcgccgt ccttcgtgac cgagcgagat gccgggtcca
     4201 ggacgaggtc gccgatcgtg atcggcgccc gccgcgaccc cgcggcgcgg ccgcggcgct
     4261 ggatcgcgcg gatccgagcc acgagctctc ctgccgagaa cggcttgacg acgtagtcgt
     4321 cggcgccgag ctcgaggccg acgatccggt cgacctcctc accccgggcg gtgagcatca
     4381 cgatcggcac gtccgagtga gctcgaacat cg
//
LOCUS       DASUVE010000146         5463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_823476, whole genome shotgun sequence.
ACCESSION   DASUVE010000146 DASUVE010000000
VERSION     DASUVE010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823476"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VF036_03070"
     CDS             complement(<1..323)
                     /locus_tag="VF036_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6264242.1"
                     /translation="MSDAVRAVVVAAHPTVRRVVEVACREAGVLVVDASGTAGEATAA
                     CRSHGPELLVLDLDLPDADGFRVLDDLGDLRPPSILVLAERADGDQVLRALRLGAGGY
                     LTKAGG"
     gene            complement(341..1006)
                     /locus_tag="VF036_03075"
     CDS             complement(341..1006)
                     /locus_tag="VF036_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6264243.1"
                     /translation="MDTTIRILVVAESMGLAQDLLIALRRRSGFDVLGPLSDESAVIE
                     SLAEARPDLVVVDLDRSDGRGLLILAAIRDEANVPAMVATRDTSAGHVELALVAGACG
                     VLPVDRQPASLASSFRRAVAGELVLPVDDLSASVGRLREARTRRGRHAALATLTQRER
                     EVIAGFARGVSISGIAVELGISRSTVQTHVKNILRKLGVHSTIEAVGVAWRAGLAVDT
                     RSA"
     gene            1079..3085
                     /locus_tag="VF036_03080"
     CDS             1079..3085
                     /locus_tag="VF036_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX6264244.1"
                     /translation="MNRLAGETSPYLLQHAGNPVDWSPWDEEALRRARIEDRPIFLSI
                     GYAACHWCHVMERESFEDEETAAFLNANFVPIKVDREERPDLDAIYMDAVQAMTGQGG
                     WPLSAFLTPEGQPFFAGTYFPKEAIHGTPSFRQVLEAIAEAWRDRRDDVERQGGQVVE
                     AISRTVSGVGSEEPLTDEISASALAELRAAFDERWGGFGVAPKFPQPMTLEFVLRMAV
                     RGKPDALEMLTTTLDRIASGGIHDHVGGGFARYSTDAAWHVPHFEKMLYDNAQLAQLY
                     VRAWLLTRDDRYRRVATDTLDYLLREMHHPEGGFYSSQDADSEGVEGKFFTWTWEELV
                     DLIGADVADVLGATPAGNWEGTNVLWLPGGSEPRGLDLSAPRRALFEAREARVRPGTD
                     DKVLTAWNAMAIAALAEAGRAFGEPRFIEAAEGCARFILKNLRRTDGRLLRSWREGVP
                     GRAGFCDDHALLASACLTLFSTTGSVDWFREAVVLLDALVDLFLDTERGGFFQTGSDD
                     EQLVLRPKELYDNAVPSGNSVAADVLQRVALLSGNLDRERVGVSAIRLLRDVLGRAPT
                     AFGHALCALDLYLGPSREVAIVGDLGDERTRSLVNEVHRARFLPNAIVAVGGPRDEEA
                     TEAVALLRDRPQVNGLPTAYVCERFTCRLPVTTPEDLAEQLMET"
     gene            3189..4001
                     /locus_tag="VF036_03085"
     CDS             3189..4001
                     /locus_tag="VF036_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264245.1"
                     /translation="MSVARSLLHRHRNADGGFGPHPGQPSELEPTALAAMALADQDAA
                     AWLAAAQQPDGAFGFDVGSYVNDSATGLCALAVGPGSEREKALDYLENAQAQRVESSA
                     AVPIDPSAVGWGWVGGTASWVEPTARALWALRVARRSSPRIADAVALLRDRASVGGGW
                     NYGNREVLGEELPPYSQTTAAALIGLRRLDPDLEIKGLDSLRRLWRAEAAGGLSLAMA
                     MAAFRIHEAPSDAERVGAALERLIVETELLGDGIALGWAALAASDKLPGIDG"
     gene            3998..5014
                     /locus_tag="VF036_03090"
     CDS             3998..5014
                     /locus_tag="VF036_03090"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX6264246.1"
                     /translation="MRHRHIDRRTFLRGAVAGGAIATAGGLGLRALLRDPPPWDEAAF
                     PPPGDARVAVLAATDYEGPLESTVLEGLRAIDADVEGANVLVKPNLVEFDPTTAINTE
                     PRLIAATVLALRRLGAASVRVGEAPGHRRDVQDVVTRSGLADALAEVEAPFVDLNLAG
                     IQRVSLRSRYTDLGALWLPIPVVEADVVVSMPKMKTHHWAGVTLSLKNLFGTLPGRVY
                     GWPKNILHWAGIERSILDIAGAVRPSYAIIDGIVGMEGNGPISGSPVGSGVLVFSDDP
                     VAADSVGASLMGFDPEQIAYLAEASRFLGQADPARIEQRGEDPGHLTRAFSPPPLGDG
                     ITAG"
     gene            complement(5147..>5463)
                     /locus_tag="VF036_03095"
     CDS             complement(5147..>5463)
                     /locus_tag="VF036_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264247.1"
                     /translation="GLPIVPRLFAAIAIAFFPIFVANLIFAERFRDTEDPTAAFGANL
                     LGAMVGGTLEYLSLLTGFRALLFLVAALYGTAYLLARRTAAGRSSTATSPRQERSPIL
                     DR"
BASE COUNT          875 a         1854 c         1882 g          852 t
ORIGIN      
        1 cccccggcct tggtgaggta tccgcctgca cccagccgca gggctcgcag gacctgatcg
       61 ccgtccgctc tctctgccaa caccaagatc gacggtgggc ggaggtcacc gagatcgtcc
      121 aggacgcgga agccgtcggc atcgggcaga tccaggtcca gcaccaggag ctcaggtcca
      181 tgtgagcgac atgccgcggt tgcctcgccc gccgtgccac tcgcgtcgac gacgagcaca
      241 cccgcctcgc gacacgcgac ctccacgaca cgccgaacgg tgggatgggc ggcgaccacc
      301 acagcacgca ccgcatcgct catgccgaga ggctagcccc tcacgcggaa cgcgtgtcga
      361 ccgcgagacc ggcgcgccac gccacgccga cggcctcgat cgtcgagtgc accccgagct
      421 tgcgaaggat gttcttgacg tgtgtctgca ccgtcgaccg actgatcccc agctcgacag
      481 cgatcccgct gatcgacacg ccccgcgcga agccggcgat gacttcgcgt tcccgctgcg
      541 tgagcgttgc cagcgcggcg tggcggccgc gccgggtgcg ggcctcgcgc agtcgcccga
      601 cggacgccga cagatcatcc acaggaagga cgagctcgcc tgcgaccgct cgacggaacg
      661 agctcgccag actcgccggc tgccgatcga ccgggaggac gccgcacgct cccgcaacca
      721 gggcgagctc cacgtggccg gcggacgtgt ctcgggtcgc gaccatcgca gggacgttcg
      781 cttcgtccct gatcgccgcg aggatcagga gacccctgcc gtcagagcga tccaggtcca
      841 ccaccacgag gtcgggtcgt gcctcagcga gggattcgat gaccgccgat tcatcggaga
      901 gcggaccgag cacgtcgaag cccgagcgtc tgcggagggc gatcaggaga tcctgagcga
      961 gccccatgct ctcggcgacc acgaggatcc tgatggtggt gtccatgact ctgcccggaa
     1021 catcggcatc tccgaggccg aagatgagct gttgtgtgcg ggctgggaca ctcgtctcat
     1081 gaaccgtctg gcaggcgaaa cgagtccgta cctgctccag catgcgggga atcccgtgga
     1141 ctggtcgccc tgggacgaag aggcacttcg gcgcgcacgg atcgaggatc ggccgatctt
     1201 cctgtcgatc ggctacgccg cgtgccactg gtgccacgtc atggagcgcg agtcgttcga
     1261 ggacgaagag acagcggcct tcctgaacgc gaatttcgtc ccgatcaagg ttgaccggga
     1321 ggagcggccc gatctcgacg cgatctacat ggacgccgtc caggcgatga ccggccaggg
     1381 cggatggccg ctctcggcat tcctcacccc cgaaggccag ccgttcttcg ccggcaccta
     1441 cttcccgaag gaagcgatcc acggcacacc gtcgttccgg caggtcctcg aggcgatcgc
     1501 ggaggcgtgg agggaccgcc gcgacgacgt cgagcggcaa ggcggccagg tcgtggaggc
     1561 gatctccagg accgtatcgg gcgtcggttc agaggaaccg ctcaccgacg agatcagtgc
     1621 aagcgcgttg gccgagctgc gggccgcttt cgacgaacga tggggcggct tcggcgtggc
     1681 cccgaagttc ccgcagccga tgacgctgga gttcgtcctt cggatggcgg tccgcgggaa
     1741 gcccgacgcc ctggagatgc tgacgacgac cttggatcgg atcgcctccg gcgggatcca
     1801 cgaccacgtc ggaggcgggt tcgcacggta ctccaccgac gcggcctggc acgtccccca
     1861 cttcgagaag atgctctacg acaacgcgca gctcgcacag ctctacgtcc gagcgtggtt
     1921 gttgacccgc gacgaccggt atcgccgggt cgcgaccgac acactcgact acctcctccg
     1981 cgagatgcac caccccgagg gcggcttcta ctcgagtcag gatgcggact ccgaaggtgt
     2041 cgagggcaag ttcttcacgt ggacctggga ggagctcgtc gatctcatcg gcgccgacgt
     2101 tgccgatgtc ctcggcgcca cgccggcagg caactgggag ggcacgaacg tgctgtggct
     2161 cccgggcggt tcggagccga ggggcctcga cctctccgcg ccgcgacgcg cactcttcga
     2221 ggcgagagag gcgcgggtcc ggccggggac ggacgacaag gtgctcaccg cgtggaacgc
     2281 gatggcgatc gcggccctcg ccgaagcggg ccgagcgttc ggggagcccc ggttcatcga
     2341 ggcagccgaa ggctgcgcgc gcttcatcct gaagaacctg cgccggaccg acgggcgact
     2401 cctgcgctca tggcgggaag gggttcccgg acgggccggc ttctgcgatg accatgcgct
     2461 gctggcctcg gcctgcctga cgctcttctc gaccaccggg tcggtcgact ggttccgcga
     2521 agcggtcgtg ctcctcgacg cgttggtgga tctgttcctc gacacggagc gtggtggctt
     2581 cttccagacg ggatcggatg acgagcagct cgtactccga ccgaaggagc tgtacgacaa
     2641 cgccgtgccg tccgggaact cggtggccgc cgacgtcctc cagcgagtcg ccctcctctc
     2701 cggcaacctc gatcgggagc gggtcggcgt ctcggcgatc cgcttgctcc gcgacgtgtt
     2761 gggaagggca ccgacggcgt tcgggcacgc cctgtgtgcg ctcgacctct acctcggccc
     2821 atcccgagag gtggcgatcg tcggagacct gggggacgaa cgcacccggt cgctcgtgaa
     2881 cgaggtgcac cgcgcccgct tcctcccgaa cgcgatcgtc gccgtgggcg gacctcggga
     2941 cgaggaggca accgaggcgg tggcgctcct tcgcgaccga ccgcaggtga acggcctccc
     3001 gaccgcgtac gtgtgtgaac ggttcacctg ccgcctcccg gtcacgacgc ccgaagacct
     3061 cgcagagcag ctgatggaga catgagagcc gtccatggga tcaggtgcgc cggctctgtg
     3121 aacgtcgtga tcgaccggac aacctcgctg tgtttcaccg gagaagagaa ggagccgatg
     3181 atctgatcat gtcggttgcg aggagtcttc tccaccgcca tcgcaacgcg gacggtggct
     3241 tcggccccca ccccggccag ccgagcgaac tggagcccac cgcgctcgcc gcgatggccc
     3301 tggcggacca ggacgcggcg gcgtggttgg ccgccgcgca gcaaccggat ggggccttcg
     3361 ggttcgacgt aggttcctat gtgaacgact cggcgaccgg attatgcgcg ctcgcggtcg
     3421 gtcctggctc cgagcgcgag aaggctctcg actacctcga gaacgcacag gcccagcggg
     3481 tcgagtcctc ggcagcggtc ccgatcgatc catccgcggt cggctgggga tgggtcgggg
     3541 ggacggcatc atgggtcgag cccacggccc gggcgctctg ggcgctcagg gttgcgagac
     3601 gctcctcacc tcggatcgcg gatgccgtgg ccctactccg ggaccgggca tccgttggag
     3661 gcggatggaa ctacgggaac cgggaggtcc tcggtgagga gcttccgccc tactcccaga
     3721 cgacggcggc agccctgatc ggcctgcggc gtctcgaccc cgatctcgag atcaagggtc
     3781 tcgattcgct ccggcggctc tggcgcgccg aggcggccgg cggcttgtcg ctcgcgatgg
     3841 cgatggcggc cttccggatc catgaggccc catccgacgc cgagagggtg ggcgctgcgc
     3901 tcgaacgcct gatcgtggag acggaacttc tcggtgacgg gatcgcactt ggctgggctg
     3961 cgctggcggc gagcgacaag ctccccggga tcgacggatg agacaccgcc acatcgatcg
     4021 ccggacgttc ctccgcggag cggtcgccgg aggggcgatc gcgaccgccg gtggacttgg
     4081 gctccgagct ctgctccgag acccacctcc ttgggacgag gcagccttcc cccctcccgg
     4141 cgacgcacgg gtggccgtgc tcgccgccac cgattacgag ggaccgttgg agtcgacggt
     4201 tctcgaaggc ctccgcgcga tcgatgccga tgtcgagggc gcgaacgtgc tggtgaagcc
     4261 caacctggtc gaattcgacc ccacgacggc gatcaacacc gagccgagat tgatcgccgc
     4321 caccgtcctc gccctccgtc ggctcggcgc tgcgtcggtg cgcgtcggcg aagcccccgg
     4381 ccaccgccgg gacgtccagg acgtcgtcac ccggtcggga ctcgcggatg ccctcgccga
     4441 ggtcgaagcg ccgttcgtcg atctgaacct cgcgggcatc cagcgggtgt cgctccgctc
     4501 gcggtacacg gatcttggcg ctctatggct gccgataccg gtggtcgagg cggacgtcgt
     4561 cgtctccatg ccgaagatga agacgcacca ttgggcgggt gtgacattgt ctctcaagaa
     4621 cctcttcggg actttgcccg ggcgggtcta cggctggccg aagaacatct tgcactgggc
     4681 ggggatcgag cggtccatcc tcgacatcgc cggcgccgtg cgaccgtcct acgcgatcat
     4741 cgacgggatc gtcggcatgg agggcaatgg cccgatctcg ggttcgccgg tcggatcggg
     4801 cgtcctcgtg ttctcggacg acccggtggc cgcggattcg gtcggggcat ccctcatggg
     4861 tttcgatccc gagcagatcg cctatctcgc cgaggcgagc agattcctcg gtcaggcaga
     4921 tccggctcgg atcgaacagc gtggcgagga cccgggacac ttgacacgag cgttctcgcc
     4981 cccaccgctc ggcgacggca tcaccgcagg ctagtcgcgg acgtagggcc gccgttcacg
     5041 tcgcccgcga gccgatgcag tccggtgtgt tccctcgccg gttggtcgcg gtcgtcgaga
     5101 ctccctagcg gtcgcgctcc ctgttggctc gccccctggg accgtgctat ctgtcgagga
     5161 tcgggctgcg ctcctgcctg ggagaagtgg ccgtgctcga cctcccggcg gccgtccttc
     5221 gagccaggag ataggccgtt ccgtagagcg cagcaacgag gaagaggagc gctcggaagc
     5281 cggtgagaag tgacagatac tccagggtcc cgcccaccat ggcgcccaga aggttcgcac
     5341 cgaaggcagc ggtcgggtcc tccgtgtccc ggaacctctc cgcgaagatc aggttcgcga
     5401 cgaagatggg gaagaacgcg atcgcgatcg ccgcgaacag acgagggacg atcggcaggc
     5461 cga
//
LOCUS       DASUVE010000147         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1579937, whole genome shotgun sequence.
ACCESSION   DASUVE010000147 DASUVE010000000
VERSION     DASUVE010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579937"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VF036_03100"
     CDS             <1..195
                     /locus_tag="VF036_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264248.1"
                     /translation="EVVDRLPLQGAISPAPFVEAVRSAGWSRIRLARLRDVEWSIERR
                     APWPLGWLRRRPRYAIVADS"
     gene            301..603
                     /locus_tag="VF036_03105"
     CDS             301..603
                     /locus_tag="VF036_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264249.1"
                     /translation="MDETWVTLGVMCMGLGGVVAGLVGVVLPEGRRLGALFALIAGAG
                     VGLVVIGLGALLDERNEPSELTFFLASLFGLLTVCGASWTVWRRAAGSRLSTGELA"
     gene            complement(600..1298)
                     /gene="lipB"
                     /locus_tag="VF036_03110"
     CDS             complement(600..1298)
                     /gene="lipB"
                     /locus_tag="VF036_03110"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX6264250.1"
                     /translation="MRREALLVRPEGPVPYGLANDAMHRLAERRLAGEIPDVLIVLEH
                     PPVFTGGRRARPDELLWSPDELAARGAEVHRIDRGGAFTFHGPGQLVGYPILDLGPKP
                     DVAAHLRRMEEAVIRTGEDLGLALRRREDVQTGVWHGDDKVCAIGVRLLRARVTVHGF
                     AVNCDPDLSWFDGIVACGLPGHGVTSLSRLAERNVTVAEVAPLLERHLADVFDIELVP
                     APDGIATALRPVAV"
     gene            complement(1309..>2003)
                     /locus_tag="VF036_03115"
     CDS             complement(1309..>2003)
                     /locus_tag="VF036_03115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX6264251.1"
                     /translation="EVTPVSHIRKLIGQHMVASLQTSARAWTMVEVNVDHLVKLRERA
                     KESFKAKHGVNLTYLPFVIRATCDALMTHPEVNSSLRGDEIVRHRAVHMGIAVSYDEG
                     LIVPVIEGADSMNTVGLARAIADLAGRARAHQLKPDEIQGSTFTITNPGPYGSIASVP
                     IINQPNTGILSFDAIQKRAVVIDDTIAIRSMVNISMSWDHRTIDGEIATRFLARVKQN
                     LESWDFAEDVTV"
BASE COUNT          279 a          651 c          730 g          343 t
ORIGIN      
        1 gaagtcgtcg atcggttgcc gttgcagggc gcgatctccc cggctccgtt cgtggaggcg
       61 gtccggagcg ccggctggtc caggatccgc ctggctcggc tgcgtgacgt cgagtggtcg
      121 atcgagcgac gggcaccatg gccgctcggg tggctccgcc gacgtccgcg ctacgcgatc
      181 gtcgccgact cgtaaggacc cctcatcggt gcggcgtatc gggtcccgat ctcatggcgt
      241 tccggtcgca tctcggtgac gacgctggcc tcggatgagc cggcgatcca tcatcggcgc
      301 atggatgaga catgggtcac gctcggagtg atgtgcatgg ggctcggcgg tgtcgtcgcg
      361 gggctcgtcg gagtggtgct ccccgagggc cggcggctcg gtgcgctgtt cgcgctgatc
      421 gccggcgccg gtgtggggtt ggtggtcatc gggctgggtg cgctgctgga cgagaggaac
      481 gaaccgtcgg agctgacgtt cttcctcgcc tcgctcttcg gcctcctcac ggtgtgtgga
      541 gcgtcgtgga cggtgtggcg gagggccgcg ggctcccggc tttccacggg agagctcgcc
      601 tagaccgcga cgggacggag cgcggtcgcg atcccgtcgg gtgccgggac gagctcgatg
      661 tcgaagacgt cggcgagatg acgctcgagc agcggtgcga cctccgcgac cgtcacgttg
      721 cgctccgcta gccggctgag cgaggtcacg ccgtgccccg gcagcccgca cgcgacgatc
      781 ccatcgaacc aggagagatc gggatcgcag ttcaccgcga agccgtggac ggtgacccgg
      841 gcgcggagca gccggacgcc gatcgcgcag accttgtcgt cgccgtgcca cacgccggtc
      901 tggacgtcct cgcgccgccg gagggccagg cccaggtcct ctcccgtgcg gatcacggct
      961 tcctccatcc gacgcaggtg cgccgccacg tccggcttcg ggccgaggtc gagaataggg
     1021 tagccgacga gctgtccggg tccgtggaac gtgaaagcgc cgccgcggtc gatccgatgg
     1081 acctccgcgc cgcgcgcggc cagctcgtcc ggcgaccaca ggagctcgtc gggccgagct
     1141 cggcgcccgc ccgtgaagac cggaggatgt tccagcacga taaggacgtc agggatctcg
     1201 ccggcgagac ggcgttccgc gagccgatgc atcgcgtcgt tcgcgaggcc gtacggaacc
     1261 ggcccctcgg gccggacgag tagggcttcc cgcctcatcg tgtggccttc agacggtgac
     1321 gtcctcggcg aagtcccagc tctcgaggtt ctgcttcacg cgggcgagga accgggtggc
     1381 gatctcgccg tcgatcgtcc ggtggtccca cgacatcgag atgttcacca tcgaacggat
     1441 cgcgatcgtg tcgtcgatca cgacggctcg cttctggatc gcgtcgaagg acaagatgcc
     1501 cgtgttgggc tggttgatga tcgggacgct cgcgatcgac ccgtagggac cggggttggt
     1561 gatcgtgaag gtcgagccct ggatctcgtc cggcttgagc tgatgggcgc gtgcccgacc
     1621 ggcgaggtcg gcgatcgccc gggcgaggcc cacggtgttc atcgagtccg ctccctcgat
     1681 caccgggacg atgagtccct cgtcgtagga gacggcgatc cccatgtgga ccgcgcgatg
     1741 ccgcacgatc tcgtcgcccc ggagcgagga gttcacttcc gggtgcgtca tcagggcgtc
     1801 gcacgtcgcg cggatcacga aggggaggta cgtcaggttg acgccgtgct tcgccttgaa
     1861 ggactccttc gcccgttcgc ggagcttcac caggtggtcg acgttcacct cgaccatcgt
     1921 ccacgcacgc gccgaggtct ggagggaggc caccatgtgc tggccgatca gcttccggat
     1981 gtgcgagacg ggggtgacct cct
//
LOCUS       DASUVE010000148         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1204715, whole genome shotgun sequence.
ACCESSION   DASUVE010000148 DASUVE010000000
VERSION     DASUVE010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204715"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VF036_03120"
     CDS             <1..600
                     /locus_tag="VF036_03120"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6264252.1"
                     /translation="PEAVPRFASQLEREAVRLSRIVADLLDLSRLESGSALDEIVSLG
                     AAAREEGKRLEESAEQAGVTLQIRTESEQPIRGSQRDVALLVRNLIDNAIRHSHEGAR
                     VTVRVDSEGQEVTLSVSDTGIGIPSRDVPRIFERFYRVDRARSRETGGTGLGLAIVKH
                     VVENHGGSVDVESELGRGTTFRVRLPGAPPDETDGPLPT"
     gene            complement(597..2141)
                     /locus_tag="VF036_03125"
     CDS             complement(597..2141)
                     /locus_tag="VF036_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX6264253.1"
                     /translation="MEARRRPRQDDQPFAVVDVGSNSARMIVFRLRPGEHLDVLEDAR
                     APLRLGRELRDDDSLGSDSIDRTIETLRDFKAIADGAGADRMIAVATAAVREASDGER
                     LVEQARALGIPLEVIDGDLEARFGFLGAVHDLPVVDGAAMDVGGGSMELTTFHDRRLQ
                     RSWTFPFGSLRVSDRFLASDPPTEREMKDLRRSAAGTFEEAGVPALDPGGDLVGIGGT
                     VRNLAKIDLRRTDHPLPLLHGYELRESNLGRIIEDLSARTMKRRARLRGLNPDRADSI
                     VGGALVVLAVMRHLGAEHVVVSSRGLREGVALGDVSVIPTPRQVRTISAATLAARFAT
                     WDVVVADRRSSLAAQLIHALDPDAAPLLAEMLEHAATLLDMGKAIDHYERFEHAAMIV
                     TTADLAGFTHEALGILSAILRHAGGDTSLGPYARLIPAGDRPAVRRAAVALALAEELN
                     RRIPPREPAPLRCTWGDRFEVEAPVPASWKPRSVTERFRAVFGARLSIVPAPDTRPLH
                     PVMPPD"
     gene            2235..>2335
                     /locus_tag="VF036_03130"
     CDS             2235..>2335
                     /locus_tag="VF036_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011604000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEX6264254.1"
                     /translation="MTTLFLVRHGLTAVTGTRLYGRTKGIHLDERGRR"
BASE COUNT          367 a          798 c          810 g          360 t
ORIGIN      
        1 cccgaggcag ttcctcgctt cgcatcgcag ctcgaacgcg aggcggtccg tctctcccgc
       61 atcgtggccg accttctcga tctctcgaga ctggaatccg ggagcgccct cgacgagatc
      121 gtctccctcg gggccgccgc ccgggaggaa gggaagcggc tggaggagtc ggcggaacag
      181 gcgggcgtga cattgcagat ccggaccgag agtgaacagc cgatccgcgg atcccagagg
      241 gatgtcgcgc tgcttgtccg caatctcatc gacaacgcga tccgccacag ccatgaaggg
      301 gccagggtca cggtccgtgt cgactcggag gggcaggagg tcaccctgag cgtcagcgac
      361 accgggatcg ggatcccgtc gcgagacgtc ccccggatct tcgagcgctt ctatcgggtc
      421 gaccgggctc ggtcgcgaga gacaggcggc acgggactcg ggctcgcgat cgtgaagcac
      481 gtggtcgaga accatggagg cagcgttgat gtcgagagcg agctcggtcg cgggacgacg
      541 ttccgtgtca ggctccccgg cgcgccgccg gatgagacgg acggaccgct tccgacctag
      601 tcgggtggca tgaccgggtg gagcggccgg gtgtccggcg ctggcacgat cgagagtctc
      661 gctccgaaga ccgcccggaa tcgttccgtg acgctcctgg gcttccagga tgccggaacc
      721 ggggcctcga cctcgaaccg gtccccccac gtgcatcgga gcggggccgg ttctctggga
      781 gggatgcgtc gattgagctc ctccgcgagg gcgagtgcga ccgccgctcg ccggaccgcg
      841 ggtcggtcac cagcggggat caatcgagcg tacggtccga ggctcgtgtc acctcccgcg
      901 tgacggagga tcgcggagag gatgccgagc gcttcatgcg tgaatccggc gagatccgcc
      961 gtcgtcacga tcatcgcggc gtgctcgaac cgctcgtagt ggtcgatcgc cttgcccatg
     1021 tcgagcagcg tcgccgcatg ctccagcatc tccgcgagga gcggcgcggc atcgggatcg
     1081 agggcgtgga tcagctgcgc cgcgagcgat gaccgacggt cggcgacgac gacgtcccac
     1141 gtcgcgaagc gcgccgcgag tgtggccgcc gagatcgtgc gcacctgtcg gggtgtcggg
     1201 atgacggaca cgtccccgag tgcaacgcct tcccggagcc ctcggctcga taccaccacg
     1261 tgctccgcgc cgagatgtcg catcacggcg aggacgacga gggctccccc cacgatcgag
     1321 tcggctcggt ctgggttgag tcctcggagc cgagcgcggc gcttcatcgt gcgggcggag
     1381 aggtcctcga tgatgcgccc gagattgctc tcacggagct catatccgtg cagcagcggc
     1441 agcggatgat cggtgcggcg gaggtcgatc ttcgccaggt tccgcaccgt gcctccgatc
     1501 ccgaccagat cgccgccggg atcgagtgcg gggacgcctg cttcctcgaa cgtccccgct
     1561 gccgatcggc gcaggtcctt catctcgcgc tcggtcgggg ggtccgacgc caggaaccgg
     1621 tcgctcacgc gaagtgaccc gaacgggaac gtccaggatc gttggagcct ccggtcgtgg
     1681 aaggtcgtca gctccatgct cccgccgccg acgtccatcg cggcgccgtc tacaaccgga
     1741 aggtcgtgca cagcgccgag gaagccgaac ctggcctcga ggtcgccgtc gatcacctcc
     1801 aacggaatcc ccagcgcccg agcctgctcg acgagccgct cgccgtcact cgcctctcgg
     1861 accgctgcgg tcgcaaccgc gatcatgcgg tccgcgcccg ctccatcagc gatcgccttg
     1921 aaatctcgca gagtctcgat cgttcggtcg atcgagtcag acccgaggct gtcgtcgtcg
     1981 cggagttccc gaccgaggcg gagcggcgcg cgcgcgtctt cgagcacatc gaggtgctcc
     2041 cccgggcgca gccggaagac gatcatccgc gccgagttcg atccaacgtc gacgaccgcg
     2101 aacggctgat cgtcttgtcg aggccgcctc ctcgcttcca cgggccgatg ctcccacacg
     2161 ggggcgggcg catcgcatcc gggaggcggt gacccgcggg atggacatgc gacccacgta
     2221 tgctcctcga cgcgatgacg accctcttcc tggtccgtca cggcctcacg gccgtgacgg
     2281 gaacgcgcct gtacggccgt acgaagggga tccacctgga cgagcgcggc cgccg
//
LOCUS       DASUVE010000149         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1016345, whole genome shotgun sequence.
ACCESSION   DASUVE010000149 DASUVE010000000
VERSION     DASUVE010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016345"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..506
                     /locus_tag="VF036_03135"
     CDS             12..506
                     /locus_tag="VF036_03135"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX6264255.1"
                     /translation="MLSLRRNPGLRSVPGSADSGQVRNERGFTLIELMTVILIIGVLM
                     LLALPTFLGARQRASDRATLADIRTAFAAERTYYVDTLTYTTDPVEMTQIEAALTYVA
                     GDTPAATHVAYLHHHPLTNTLFISAKSSIGTCWYLREIDGGGPQFASDPACGVADVQV
                     YSTL"
     gene            553..1554
                     /gene="add"
                     /locus_tag="VF036_03140"
     CDS             553..1554
                     /gene="add"
                     /locus_tag="VF036_03140"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX6264256.1"
                     /translation="MRDLTLLPKAELHVHLEGAMRVETVRELAERVGGAVPSGLVDGR
                     WGFAGFDDFISQYTAMCALLGTTEDFRRIAYEFCEDEARTGVRYAEVVFSPSNHAPRL
                     GDDWFGPIEAVLDGLEAGRRDFGVTAMLAPDLVRDAELEAAERSLEVALRFAGRGVVA
                     LNAAGSERSRIAPFGPLFRRAKNGGLRSVPHAGEWAGPHNVWETLEHYLPDRIGHGVR
                     AIEDPALVEHLAATGIPLEISPVSNVATGVYGSLAEHPFPALRDAGVTVTLNSDDPPM
                     FGAWLTDVYRSARDTWSFDDVDLAEIARTGVRASFADDDVKAEILAGIDAWLTEPTA"
     gene            complement(1562..2440)
                     /locus_tag="VF036_03145"
     CDS             complement(1562..2440)
                     /locus_tag="VF036_03145"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6264257.1"
                     /translation="MSKRSRDRQLAKLAARRQAERAAALRRRNLIVGILAGVVALVVV
                     VAGLSVLLGDDTETAAPTPTPTSPSGSPSPSVEPGTKTGTVTPTAATGSGEVACGAVA
                     PAKAGKPKPQFAGPPPMEIERKATYTATMQTSCGAIVIELDPKRAPQTVNSFVFLAEA
                     GYFDGQYFHRLDTSIDVIQGGDPSGDGTGGPGYTIPDELTGDESYTPGTLAMANAGAN
                     SGGSQFFLITGPAGTNLDGNPAFTIFGKVVEGLDVAQRIQKLPIVDSDSGDISGQRPA
                     EAVYIESVTIEKSGGG"
     gene            complement(2453..>3289)
                     /locus_tag="VF036_03150"
     CDS             complement(2453..>3289)
                     /locus_tag="VF036_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEX6264258.1"
                     /translation="RDMVITRLGKGVDERGTLEPAAIERAAEAVARYRRRARALHAEQ
                     VRIGATSAVRDASNREDFFSRVRDVTGIEPEVIDGEREAALSFLGGTRGLDPAVGPFL
                     LIDIGGGSTEFVFGREPGAADHAISTQMGSVRLTERVHPSDPLTGDDLRHVETLVVEQ
                     LDEVERIVPIGEGRTLVAVAGTATTLQACALGLERYDPDLIHRSMLTLAAAERTLREL
                     AAMTNEERAAIPVMPVGRGDVIVAGASILVHAMRRFGFEHALVSETDILDGLALELLG
                     VR"
BASE COUNT          483 a         1182 c         1110 g          514 t
ORIGIN      
        1 ggctgcgtcc gatgctatcg ctccgtagga acccggggct caggtcggtc cccgggagcg
       61 ccgatagtgg gcaagtgagg aacgaacgcg gcttcacgct gatagagctg atgacggtga
      121 tcttgatcat cggcgtcctg atgctgctcg cgctcccgac gttcctgggt gcccggcagc
      181 gggcctccga ccgggcgacg ctcgcggaca tccgcacggc gttcgccgcc gaacgcacct
      241 attacgtgga caccctcacc tacacgaccg accccgtaga gatgacccag atcgaggcgg
      301 cgctcaccta cgtcgccggt gacacgcccg cggccaccca cgtggcctac cttcaccacc
      361 accccctcac gaacaccctc ttcatctccg cgaagagctc catcgggacg tgctggtacc
      421 tgcgagagat cgacgggggc ggccctcagt tcgcctccga tcctgcgtgc ggcgtcgccg
      481 acgtgcaggt gtattccacc ctctgagatc gctatctcgc gcgaccgcgt tctccccgct
      541 tagcatccgc ggatgcgcga cctcaccctc ctccccaagg cggagctcca tgtgcacctc
      601 gagggggcga tgcgcgtcga gaccgtccgc gagctcgcgg agcgagtggg cggggcggtc
      661 ccgtccgggc tcgtggacgg ccggtggggg ttcgcgggct tcgacgactt catctcgcag
      721 tacacggcga tgtgcgcgct gcttggaacc accgaagact tccgacggat cgcctacgag
      781 ttctgcgagg acgaggcccg caccggcgtc cggtacgccg aggtcgtgtt ctccccgagc
      841 aaccacgctc cccgactcgg cgacgactgg ttcggaccca tcgaggccgt gctggacgga
      901 ctcgaggcgg gtcggcgcga cttcggtgtg acggcgatgc tcgcgcccga tctcgtgcgc
      961 gatgcggagc tcgaggcggc ggaacggtcg ctggaggtcg cactgaggtt cgcgggtcga
     1021 ggtgtggtgg cgctcaacgc cgcggggagc gagcggagca ggatcgctcc attcggcccc
     1081 ctgttccggc gtgccaagaa cggcgggctg cgatccgtgc cgcacgccgg tgagtgggcg
     1141 ggaccgcaca acgtgtggga aacgctcgag cactacctcc ccgaccggat cggacacggc
     1201 gtgcgggcga tcgaggaccc cgcgctcgtc gagcacctcg ccgccaccgg catccccctc
     1261 gagatctcgc cggtctccaa cgtcgcgacc ggcgtctacg gctccctcgc ggagcatccg
     1321 ttcccggcgc tccgcgacgc cggagtcacc gtcaccctga attccgacga cccaccgatg
     1381 ttcggtgcgt ggctcaccga cgtctatcgg agcgcacggg acacctggag tttcgacgac
     1441 gtcgacctcg ccgagatcgc ccgcaccggc gtgcgcgcat ccttcgcgga cgacgatgtc
     1501 aaggcggaga tcctcgcggg gatcgacgcg tggctgacgg aacccacggc ctgacggccc
     1561 ctcagccgcc gccggacttc tcgatcgtca ccgactcgat gtagacggcc tccgcgggcc
     1621 gctgcccgga gatgtcgccg gaatcggagt ccacgatcgg aagcttctgg atccgctgcg
     1681 cgacgtcgag gccttcgacc accttcccga agatcgtgaa tgccgggttc ccgtcgaggt
     1741 tggtgccggc cggaccggtg atgaggaaga actggctgcc gcccgagttc gcacccgcgt
     1801 tcgccatcgc gagggtgccg ggcgtgtagg actcgtcccc ggtcagctcg tcggggatcg
     1861 tgtagccggg ccctccggtg ccgtctcccg atggatcgcc accctggatc acgtcgatcg
     1921 aggtgtcgag acgatggaag tactgcccgt cgaagtaccc cgcctcggcg aggaacacga
     1981 aggagttgac ggtctgaggg gcgcgcttcg gatcgagctc gatcacgatc gcgccgcacg
     2041 aggtctgcat cgtggcggtg taggtcgcct tccgctcgat ctccatcggc ggcggacccg
     2101 cgaactgggg cttcggcttc ccggccttgg ccggtgcgac cgccccgcac gcgacctccc
     2161 ccgacccggt ggccgccgtg ggcgtgacgg tccccgtctt cgtcccgggc tccaccgatg
     2221 gagacggact tcccgaggga ctcgttggcg tcggggtggg agcggccgtc tccgtgtcat
     2281 cgcccaacag gacgctgagt cccgccacca ccacgaccag cgccacgacc ccggccagga
     2341 tcccgacgat caggttgcgt cggcggagag ctgccgcgcg ctcagcctgt cgtcgagcgg
     2401 cgagcttcgc gagctgacga tccctactcc gcttcgacat cggcggtcga ggttaccgga
     2461 cgcccaacag ctcgagggcg aggccgtcga ggatgtcggt ctcgctgacc agggcgtgct
     2521 cgaagccgaa ccgccgcatc gcgtgcacga ggatcgaggc ccccgcgacg atcacgtcgc
     2581 cgcgacccac cggcatgacc gggatcgcgg cgcgctcctc gttcgtcatc gcggcgagct
     2641 cccggagcgt gcgctcggcg gcggcgagcg tgagcatcga gcgatggatc aagtcgggat
     2701 cgtaccgctc gagcccgagc gcacacgctt ggagcgtcgt cgcggtcccc gcgaccgcca
     2761 cgagcgtgcg gccctccccg atgggcacga tgcgctcgac ctcgtcgagt tgctcgacga
     2821 cgagcgtctc gacgtgccgg aggtcgtcgc cggtgagcgg atcggacggg tgcacccgtt
     2881 cggtgagccg cacgctgccc atctgcgtcg agatcgcgtg atccgcggcg ccgggctccc
     2941 tcccgaagac gaattccgtg gaaccgccac cgatgtcgat caggaggaac ggaccgacgg
     3001 caggatcgag gccccgggtc cctcccagga acgagagcgc cgcctctcgt tcaccgtcga
     3061 tgacctcggg ctcgatcccc gtcacgtcgc gcacgcgcga gaagaagtct tcgcggttgc
     3121 tcgcgtcgcg gacggccgac gtggccccga tccggacctg ctcggcgtgg agcgccctcg
     3181 cccggcgacg gtaccgcgcg accgcttccg ccgcacgctc gatcgccgcc ggctcgagcg
     3241 ttcctcgctc gtcgacgccc ttccccagcc gcgtgatgac catgtcgcg
//
LOCUS       DASUVE010000150        10112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_640900, whole genome shotgun sequence.
ACCESSION   DASUVE010000150 DASUVE010000000
VERSION     DASUVE010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10112
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640900"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VF036_03155"
     CDS             <1..678
                     /locus_tag="VF036_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264259.1"
                     /translation="RVRAARTADASPLVLLDLAVPRDVEPEAGALPGVTLFDVDALRG
                     ADLVLSATGATGVVVHAEQVRAARTADASPLVLLDLAVPRDVEPDAGALPAVTLFDVG
                     ALRDRGSAEEDEAVERARGIVAEEVRRFVVRRRGDELSPVIRAIRRRGDAVLESEVAR
                     HASRLADLTPDEIAAVEALARGIVAKLLHDPIVALKERSEAGSERTHAALLVDLLSLD
                     PSLLDEE"
     gene            675..1580
                     /gene="hemC"
                     /locus_tag="VF036_03160"
     CDS             675..1580
                     /gene="hemC"
                     /locus_tag="VF036_03160"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX6264260.1"
                     /translation="MTTLRIATRRSALALAQAEEVAALLSANAFRSEIVPMSTWGDEG
                     VASTESPSGMKGLWVDQIVEALRTGAVDVAVHSAKDLPAEDEDDIVIAAVPKRADPLD
                     VLIGREATLPPGVVVGTSSVRRRAQLLAGFPGMGVTEIRGNVDTRLRKLADREVDGLV
                     LAAAGLARLGIDPPNSRRLRVDEMVPAPGQGSLAVQVRAEDRTTRAVVSVLDHLPSRR
                     SLEAERALVQLLGGGCDLPLGALAAAKGDRIRMVALVATPDGAKVVRSASEGTDPSIV
                     ASALAQRLRADGADRILAEVQGTPE"
     gene            1577..2335
                     /locus_tag="VF036_03165"
     CDS             1577..2335
                     /locus_tag="VF036_03165"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX6264261.1"
                     /translation="MKRTLAGRTIVVTRPAEQAEPLVRALERRGARVLIAPTIRIVPG
                     RSAALTSALKELATGRFDWITLTSRATVHVLRDRLASPRGVRAKVAVIGDGTAEEFRR
                     WTRRDPDLQPSTFTTSALARAFPRGAGRVLCARADIAPAGLETALAAKGWETERVDAY
                     RTVFATSLPREARDAFRRGEVDAVTFTSASTVLGFANSLGAAKGSPKVVCIGPVTAAA
                     ARDAGLRTAAVARPHTVEGLVAALERALVSTGPR"
     gene            complement(2332..3423)
                     /locus_tag="VF036_03170"
     CDS             complement(2332..3423)
                     /locus_tag="VF036_03170"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX6264262.1"
                     /translation="MEPFVYPLTDEQDERAERLLRESVCISLHDHCGITPVDMTQNDE
                     YIREGREWYGYEGLAASGLDAVFENFLDGTATITSKGGWKWTDVIHDLGMHLADVAHQ
                     STVFVGGTVDDIVEAHRSGRIAMVPCMEGAAMIENELDRLHVLFGLGVRMCGVAYSES
                     NQLGGGVKDPGDGGLTRFGLDAVRRMNRLGMAIDVSHTGDVTAMQTCEVSERPVFLSH
                     SGARALFSERKLKEDVLLKAVAENGGVIGIEGSPHTTISPAHPRHSLEGVMDHFEYCI
                     DVMGMDHVGFGPDTFFGDHVGLHVLYAGNLDTGEVGEYEPVAYVEGMENPSEFPNVIR
                     WLVGHDYTDEQIAKVVGGNALRALREVWV"
     gene            3577..4557
                     /gene="hemB"
                     /locus_tag="VF036_03175"
     CDS             3577..4557
                     /gene="hemB"
                     /locus_tag="VF036_03175"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX6264263.1"
                     /translation="MSFPDSRPRRMRRTPALRSLIRETDVRPGDLIAPLFVKEGLTEP
                     APITSMPGQSQHTIESLRKEAVEIAERGVLAFILFAVPTRKDPEGSEAWNPEGIGQQA
                     IAALRSEFGDEAVVIGDLCLDEYTDHGHCGVLNDLGEVDNDATVELYQRIALAQAEAG
                     AHLVGPSGMMDGQVAAIRDAMDAAGHQDIGILAYAAKFASALYGPFREAAEGAPRFGD
                     RTGHQEDPANLQEALREIRTDIDEGADIVMVKPALPYLDVIRRAKDETRFPMAAFHVS
                     GEYAMVKAAAANGWLDERRVVLELLTSIRRAGADLILTYHAKDAADWLRG"
     gene            4570..5124
                     /locus_tag="VF036_03180"
     CDS             4570..5124
                     /locus_tag="VF036_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX6264264.1"
                     /translation="MPRYVALLRGINVGGKNPVPMPVLKACFEDGGFEDVGTYIQSGN
                     VVFTGPSSSQAALTTRIERTIRKAFAHYEASVVVKSRSQMRAVVDKAPKGFGRDPAKY
                     RYDVCFLKPSVTAKQVVASLPTKEGVDRIWAGTGVVYFSRLTSRATSSRLNRVASLPV
                     YQQMTIRNWNTTTKLRELLDAEIA"
     gene            complement(5137..6066)
                     /locus_tag="VF036_03185"
     CDS             complement(5137..6066)
                     /locus_tag="VF036_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX6264265.1"
                     /translation="MTVGSDDVRAAAERIAGRIRRTPVLELGELRGASVVAKLELLQH
                     TGSFKPRGAFNKLLSSEVPPASVIAASGGNFGLAVAYAARETDVLAEVFIPSTSPTTK
                     VERVRAHGANVTIVDGYWAEAQEAASARAAEIGALLMHPFDQPDVIAGQGTLGLEIEE
                     QVPDVDTILMGVGGGGPLAGIAAWCGDRVRVIGVEPEGCASMTAALAAGEPVMVEVGG
                     LGADSLGAARVGDHVFPIVRDRVDRMALVTEGQIREAQRALWEDARLIVEPGGAAAFA
                     ALRSGAFDVSPGERVGVVLSGGNCDPSTVTSLT"
     gene            6105..7379
                     /gene="hemL"
                     /locus_tag="VF036_03190"
     CDS             6105..7379
                     /gene="hemL"
                     /locus_tag="VF036_03190"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX6264266.1"
                     /translation="MDGSGELFERAKRSIPGGVNSPVRAFGAVGGTPRFAVRGEGASF
                     EDEDGRWYVDLCQSWGASIFGHARKEIVDASAEAASRGTSFGIPTEAEVLLAERIVSM
                     VPSIEQVRLVSSGTEAAMSAIRLARAATGKDALIKLEGCYHGHSDALLAHGAGSGLAT
                     FGIPSTPGVTAGATRDTLVAPFNDLDGVAALLDANEGAVAALIVEPVPANMGVVPPDD
                     GYLEGLRLLCDEHRALLIFDEVITGFRIARGGAQERFGVTPDLTVLGKVMGGGFPSAA
                     FGGRRDVMEQLAPAGHVYQAGTLSGNPVAVAAGLAALDLIDNENPYPGLETTAEDLQA
                     GLAAAFEANDIGATINRAGSLFSVFFSDGPIRNFTDAKAADHDRFARFFHHMLDEGVY
                     LPPSGYELWTLCSAFGEAEREAVLGAAGRFRD"
     gene            complement(7392..7835)
                     /locus_tag="VF036_03195"
     CDS             complement(7392..7835)
                     /locus_tag="VF036_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264267.1"
                     /translation="MRQWELIGITDTVDWSPPRRDPVPSDDDGDHFPAVLWTTDGSLR
                     LRGVSWVASQHLGIHPRFLDGRELLEAFGMEGESLPILEAHAAALTGKTVTFSLPGIE
                     GRMWCRVAPTHDAGEHIVGTFCIATEPASDGDELETVQDTTLTLV"
     gene            8031..8546
                     /gene="msrA"
                     /locus_tag="VF036_03200"
     CDS             8031..8546
                     /gene="msrA"
                     /locus_tag="VF036_03200"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008898308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX6264268.1"
                     /translation="MPSEIATFGAGCFWGVEHLFRRVPGVTGAISGYSGGFVENPSYQ
                     LVCTDTTGHAEVVQVTFDPEVVSYDQLLAVFWAMHDPTQVNRQGPDRGSQYRSIVLTH
                     SDEQRTAAEASRDRVQSRYERPIATEIQPFVAFYPAEDYHQRYYLNNGHEPYCHSIPP
                     GLLEDLGLVRT"
     gene            8581..9399
                     /locus_tag="VF036_03205"
     CDS             8581..9399
                     /locus_tag="VF036_03205"
                     /inference="COORDINATES: protein motif:HMM:NF016334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX6264269.1"
                     /translation="MTEASARPNDLRPFLDRFVAACAQDDRIVAAFLGGSRGRGEADV
                     YSDVDLCVIASNDAYEALIRDRAAFVGRLGTPLFLEDFGFSDLVFFILDDGTEGELFL
                     GSEERLDELEPGPTIRTLFDPGGILEGVVFAEERMDRTEQREVLRQILTWFWHELSHF
                     ITAMGRGELWWAAGQLESLRGHCVNLVRAEHGSFAGEEPYEKLDRTIDLPALSPIAET
                     FVPMERDTLLRAARELVRFYTEKAPGVAEANGATYPLELEKLMVKRLNDLGGSR"
     gene            complement(9396..10058)
                     /locus_tag="VF036_03210"
     CDS             complement(9396..10058)
                     /locus_tag="VF036_03210"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX6264270.1"
                     /translation="MSDPEIRIEENGPYEVKGPLPIARTRTVKTEFGEPVDYAPFEPL
                     EADETYRLCRCGKSGNKPFCDDSHLEEPRVDVTESADHGPRDDRTDVFQGPDGAIISD
                     DLSICTKAGYCKDRFTGVWQMLGDPAVRERMGRMVQLCPSGRLAWADSVDAPDHEPEY
                     EPEIGVFRNGPLFVRGGVKVVGADGEPYEVRNRQALCRCGYSQNKPFCDGSHKLVGFR
                     DG"
BASE COUNT         1531 a         3580 c         3455 g         1546 t
ORIGIN      
        1 cgggtccgtg cggctcggac agctgatgcg tccccgctcg tgctgctcga cctcgccgtg
       61 ccgcgcgacg tcgagcccga ggccggcgca ctcccgggcg tgacgctctt cgacgtcgac
      121 gcgttgcgag gggcggacct cgtcctctcg gccacgggag cgaccggcgt ggtggtccac
      181 gcggagcagg tccgtgcggc tcggacggct gatgcgtccc cgctcgtgct gctcgacctc
      241 gccgtgccgc gcgacgtcga gcccgatgcc ggcgcactcc cggccgtgac gctcttcgac
      301 gtcggcgcgc tgcgcgaccg tggttccgcg gaggaggacg aggcggtcga gcgagcgcgc
      361 gggatcgtcg ccgaggaggt gcgccggttc gtggtgcgtc gccgcggcga cgagctctcc
      421 ccggtgatcc gcgcgatccg tcgccgtggc gacgcggttc tggagagcga ggtcgcgcgg
      481 cacgcgagcc gcctcgccga cctcaccccc gacgagatcg cggcggtcga agccctcgcg
      541 cgagggatcg tcgcgaagct cctccacgac ccgatcgtgg cgctgaagga acggtccgaa
      601 gcgggctcgg agcgcacgca cgcggccctc ctcgtcgatc tcctcagcct cgacccttcc
      661 ctcctcgacg aggagtgacc accctccgga tcgcgacgcg gcgctccgcc ctggcgctgg
      721 cgcaggccga agaggtggcc gcgctgctgt ccgcgaacgc cttccgatcg gagatcgtcc
      781 cgatgtcgac gtggggcgac gagggggtgg catccaccga gtcgccgtcc gggatgaagg
      841 gtctctgggt cgaccagatc gtggaggcgc tccgcacggg tgcggtggac gtcgcggtcc
      901 attccgcgaa ggacctccct gccgaggacg aggacgacat cgtgatcgcc gccgtcccga
      961 agcgcgccga cccgctcgac gtcctgatcg gccgcgaggc caccctccca ccgggggtcg
     1021 tggtgggcac ctccagcgtc cggcgtcgcg cccagctgct cgccggtttc cccggcatgg
     1081 gagtcacgga gatccgcggc aacgtcgaca cccggctccg gaagctcgcc gaccgtgagg
     1141 tggacggact cgtgctcgcg gcggcggggc tcgcgcgcct cgggatcgac ccgccgaact
     1201 cccggcgtct ccgtgtggat gagatggtgc cggcaccggg ccagggaagc ctcgccgtgc
     1261 aggtgcgcgc cgaggatcgg accacgaggg ccgtcgtctc cgtgctcgat cacctgccct
     1321 cgcgccgctc gttggaggcg gaacgggccc tcgtgcagct cctcggcgga ggctgcgacc
     1381 tcccactggg ggcgctcgcc gcggcgaaag gggaccggat ccgaatggtg gcgctcgtcg
     1441 cgacgcccga cggcgcgaag gtggtgaggt cggcgtccga gggcaccgat ccgagcatcg
     1501 tcgcgtcggc cctcgcacag cgcctgcggg ccgacggcgc cgaccggatc ctcgcggagg
     1561 tgcaggggac gccggagtga aacgcaccct cgccgggcgg acgatcgtcg tcacgcgccc
     1621 ggccgagcag gcggagccgc tggtacgagc gctcgagcgg cgcggagcac gcgtcctgat
     1681 cgcaccgacg atccggatcg ttcccgggcg ctccgcagcg ctcacttcgg cattgaagga
     1741 gctcgctacc ggccggttcg actggatcac gctcacgagc cgggcgaccg tccacgtgct
     1801 ccgcgatcgg ctcgcgtcgc cgcgcggcgt ccgcgcgaag gtcgcggtga tcggcgacgg
     1861 caccgcagag gaattccgtc gctggacacg gcgcgatccc gatctgcagc cgtcgacgtt
     1921 cacgacctct gcgctcgcgc gcgcctttcc acgaggggcg ggacgcgtgc tctgcgcacg
     1981 cgccgatatc gccccggcgg gattggagac ggcgctggca gcgaagggtt gggagaccga
     2041 acgggtcgac gcctaccgga cggtcttcgc cacgtccctc ccgcgcgagg cgcgcgacgc
     2101 gttccgccgc ggggaggtcg acgccgtgac gttcacgagc gcctccacgg tcctaggctt
     2161 cgccaactcg ctcggcgccg cgaagggatc cccgaaggtg gtctgcatcg gtccggtcac
     2221 cgcggcggca gctcgcgacg ccgggctccg cacggcagcc gtcgcacggc cccacacggt
     2281 cgagggcctc gtcgcggccc tcgagcgagc gctcgtgtcg accgggccgc gttagaccca
     2341 gacctcgcgc agcgccctca gcgcgttgcc cccgacgacc ttcgcgatct gctcgtcggt
     2401 gtagtcgtga ccgacgagcc accggatcac gttcgggaac tccgagggat tctccatccc
     2461 ctcgacgtag gcgaccggct cgtactcgcc gacctccccg gtgtcgaggt tgcccgcgta
     2521 caggacgtgg agccccacgt ggtcgccgaa gaacgtgtcg ggaccgaacc cgacatgatc
     2581 catccccatc acgtcgatgc aatactcgaa gtggtccatc accccttcca gcgaatgccg
     2641 cgggtgcgcc ggcgagatcg tcgtgtgcgg agatccctcg atcccgatca cgccgccgtt
     2701 ctccgcgacc gccttcagca gaacgtcctc cttcagcttg cgttcggaga acagtgcccg
     2761 cgcgcccgaa tgcgacagga agaccggtcg ctcgctcacc tcgcaggtct gcatcgcggt
     2821 gacgtcgccc gtgtgcgaga cgtcgatcgc catgccgagc cggttcatcc tccgcacggc
     2881 gtcgaggccg aatcgggtga ggccgccgtc gccgggatcc ttcacaccgc cgccgagctg
     2941 gttcgactcg ctgtaggcga caccgcacat ccggacgccg aggccgaaga gcacgtggag
     3001 acggtcgagc tcgttctcga tcatcgcggc gccctccatg cagggcacca tcgcgatccg
     3061 gccgctccgg tgcgcctcca cgatgtcgtc gacggtgccg ccgacgaaca ccgtcgattg
     3121 gtgcgcgacg tctgccaggt gcatcccgag gtcgtggatc acgtcggtcc acttccatcc
     3181 gcccttcgac gtgatcgtgg ccgtcccgtc gaggaagttc tcgaagaccg cgtccagtcc
     3241 ggaggcggcc agtccctcgt agccgtacca ctccctaccc tcgcggatgt actcgtcgtt
     3301 ctgcgtcatg tcgacgggcg tgatgccgca atggtcgtgc agggagatgc acacgctctc
     3361 gcggagcagc cgttccgcgc gttcgtcctg ctcgtcggtg agcgggtaca cgaacggctc
     3421 cacgcgaccg agctgcgggg aaagctcgaa ctccttgtag tcgacacccg gccaggtacg
     3481 agaacgacac gtagccgtcc catcgcttct tcggctccat cctcccctcc cctcgctcgt
     3541 cggcctcgtg atctcggccg cggtacgctc tggcccgtga gcttccctga ttcccgaccg
     3601 cggcgcatgc gccggacccc cgcgctccgt tcgctgatcc gcgagaccga cgtgcggccg
     3661 ggcgacctga tcgcgcccct cttcgtcaag gaagggctca ccgagcctgc accgatcacg
     3721 tcgatgccgg gccagtcgca gcacacgatc gagtcgctcc gcaaggaagc cgtcgagatc
     3781 gcggagcgcg gtgtgctcgc gttcatcctc ttcgcggtac ccacgcggaa ggatcccgag
     3841 gggtcggagg cctggaaccc cgaggggatc ggtcagcagg cgatcgcggc gctccggtct
     3901 gagttcggcg acgaggccgt ggtgatcggc gacctctgcc tcgacgagta caccgaccac
     3961 gggcattgcg gcgtcctcaa cgacctcggt gaggtggaca acgacgcgac ggtggagctc
     4021 taccagcgga tcgcgctcgc gcaggccgag gcgggcgccc acctcgtcgg ccccagcggg
     4081 atgatggacg gccaggtcgc cgcgatccgt gacgcgatgg acgcggccgg tcatcaggac
     4141 atcggcatcc tcgcctacgc ggcgaagttc gcttcggcgc tgtacggccc gttccgcgag
     4201 gcggccgagg gcgcgccccg gttcggggac cgcaccggcc accaggagga tcccgcgaac
     4261 ctgcaggagg ctctccggga gatccgcacg gacatcgacg agggcgccga catcgtgatg
     4321 gtgaagccgg cgctgccgta cctcgatgtg atccggcgcg cgaaggacga gacgaggttc
     4381 ccgatggccg cgtttcacgt gagcggtgaa tacgcgatgg tgaaggcggc ggccgcgaac
     4441 ggctggctcg acgagcggcg cgtggtgctc gagctgctga cgtcgatccg tcgggccgga
     4501 gcggatctga tcctcaccta ccatgcgaag gacgccgcgg actggctccg gggctgagcc
     4561 cgagcgtcga tgcctcgcta cgtcgcgctc ctccgcggca tcaacgtcgg cgggaagaac
     4621 ccggttccga tgccggtgtt gaaggcatgc ttcgaggacg ggggcttcga agatgtcggg
     4681 acctacatcc agagtggcaa cgtcgtgttc accggtccgt cctccagcca ggccgccctg
     4741 accacgagga tcgagcgaac gatccggaag gcgttcgctc actacgaggc gagcgtcgtc
     4801 gtgaagagcc ggagccagat gcgcgcggtc gtcgacaagg cgccgaaggg cttcggccgg
     4861 gatccggcga agtaccgcta cgacgtgtgc ttcctgaagc cgtccgtcac ggcgaagcag
     4921 gtggtggcca gcctcccgac gaaggagggt gtggaccgca tctgggcggg aacgggcgtc
     4981 gtctacttct cgaggctcac gagccgcgcg acgtcgagcc gcctgaatcg agtggcgtcg
     5041 ctacccgtct accagcagat gacgatacgg aactggaaca cgaccacgaa gctgcgcgag
     5101 cttctcgacg cggagatcgc ctgaggtccc gctcggctac gtcagagacg tgacggtcga
     5161 gggatcgcag ttcccaccgg agagcacgac gcctacgcgc tcgccgggcg agacgtcgaa
     5221 ggcacccgat cgcagggccg cgaacgctgc cgccccgccg ggctcgacga tcaggcgggc
     5281 gtcctcccaa agggcccgct gcgcctcacg gatctgacct tccgtcacca gggccatccg
     5341 gtcgacccga tcgcgcacga tcgggaagac gtggtcacct acccgtgcgg cgccgagcga
     5401 atccgcaccg aggccgccga cctccaccat gaccggttcg ccggccgcga gcgccgccgt
     5461 catcgacgcg cagccttcgg gctcgacgcc gatcacccga acccggtcgc cgcaccacgc
     5521 agcgatcccc gcgagcggtc caccgccgcc gacgcccatc aggatcgtgt cgacatcggg
     5581 cacctgctcc tcgatctcga gcccaagcgt cccctgaccg gcgatgacgt cgggctgatc
     5641 gaacgggtgc atcaggagcg cgccgatctc ggctgcacga gcgctcgccg cctcctgcgc
     5701 ctcggcccag tagccgtcga cgatcgtcac gttcgcgccg tgggcgcgga cgcgctccac
     5761 cttcgtcgtc ggcgaggtcg acgggatgaa gacctcggca aggacgtcgg tctcgcgggc
     5821 agcataggcc acggcgaggc cgaagttccc accggaggcg gcgatcacgc tggccggcgg
     5881 tacctcactc gacagcagct tgttgaacgc tccgcgcggc ttgaacgacc ccgtgtgctg
     5941 caacagctcg agctttgcca cgaccgatgc accgcgcaac tcaccgagct cgagcacggg
     6001 cgttcgacgg atccgaccgg cgatccgttc ggcggcggcg cggacatcat cgctcccgac
     6061 ggtcatgggg tcgcatccaa ccacagccat agactctccg gccgatggac ggatccggcg
     6121 agctgttcga acgcgcgaag cgatcgatcc ccggcggggt gaattcgccg gtccgggcgt
     6181 tcggcgccgt cggtggcacg ccacggttcg ccgtccgagg cgagggtgcc tcattcgagg
     6241 acgaggacgg ccgctggtac gtcgatctct gccagtcgtg gggcgcctcg atcttcggcc
     6301 acgcgcggaa ggagatcgtg gacgcctccg cggaggcggc ttcccgaggg acctcgttcg
     6361 gcatccccac cgaagcggag gtcctcctcg ccgagcggat cgtgtcgatg gtgccttcga
     6421 tcgaacaggt tcgactcgtg tcctccggca ccgaggcggc gatgtcggcg atccgactgg
     6481 cgcgcgccgc caccgggaag gacgcgctga tcaagctcga gggctgctac cacggtcatt
     6541 ccgacgccct gctcgcgcac ggcgcgggta gcggtctcgc gacgttcggg atcccgagca
     6601 cgcccggcgt caccgccggg gccacgcgcg acaccctggt cgcgcccttc aacgacctcg
     6661 atggggtcgc ggcgctcctc gatgcgaacg aaggggccgt cgccgcgctc atcgtcgagc
     6721 cggttcccgc gaacatgggc gtggttccac ccgacgacgg gtacctcgag gggctccgtc
     6781 tgctctgtga cgagcaccgt gcgctcctga tcttcgacga ggtgatcacc gggttccgga
     6841 tcgcccgggg cggcgcgcag gagcgcttcg gcgtcacccc cgacctcacc gtcctcggca
     6901 aggtgatggg gggcgggttc ccgagcgccg cgttcggcgg ccgacgcgat gtgatggagc
     6961 agctcgcgcc ggcgggccac gtgtaccagg cggggacgct gagcgggaac ccggtcgcgg
     7021 tggcggccgg cctggcggcg ctcgatctca tcgacaacga gaacccctac ccgggactcg
     7081 agaccaccgc ggaggatctc caggccggcc tcgccgcggc cttcgaggcc aacgacatcg
     7141 gcgccacgat caaccgagca gggtccctgt tcagcgtctt cttctcggac ggacccatca
     7201 ggaacttcac tgacgcgaag gccgcggacc acgaccggtt cgcgcgcttc ttccaccaca
     7261 tgctggacga gggcgtgtac ctcccgccat cgggctacga gctgtggacg ctctgctcgg
     7321 cgttcggcga ggcggaacga gaagcggtgc tcggggcggc cggccgcttc cgcgactgac
     7381 cgccccgacg ctcacaccag cgtgagagtg gtgtcctgca ccgtctccag ctcgtcgccg
     7441 tcggacgccg gctcggtggc gatgcagaac gtccccacga tgtgctcgcc cgcgtcgtgc
     7501 gtcggcgcga cacggcacca catgcgcccc tcgatccccg ggaggctgaa cgtgaccgtt
     7561 ttccccgtca gcgcggccgc gtgggcctcg aggatcggga ggctctcccc ttccatcccg
     7621 aacgcttcca ggagctcacg gccgtcgagg aagcgagggt ggatccccag gtgctgggaa
     7681 gctacccacg agaccccacg gaggcggagc gagccgtccg tcgtccacag gaccgccggg
     7741 aagtgatctc cgtcgtcgtc cgaagggacc ggatcacggc gaggtgggga ccagtccacc
     7801 gtgtccgtga tcccgatgag ctcccactgt cgcatgtgcc tctcctcctc ggtgcggctc
     7861 cccagcacta cccgatatcg ggtagcaacc ccttccggca tatcggcgaa gaggaccatt
     7921 tcggccttcc ccgaggggac tagggcttcc ggaggagggg cggtccctgc aacggatgca
     7981 cccaccgctg gcactatgga ggcgagagtc atcgacgaga gaggaacgac atgccaagcg
     8041 agatcgcgac cttcggcgcc ggctgcttct ggggcgtcga gcacctgttc cgaagggtcc
     8101 ccggagtcac cggagccatc tccggctaca gcgggggctt cgtcgagaac ccttcgtacc
     8161 aactcgtctg cacggacacg acgggccacg cggaggtcgt ccaagtgacg ttcgatcccg
     8221 aggtcgtctc ctatgaccag ctcctcgcgg tcttctgggc gatgcacgat ccgacccagg
     8281 tgaaccggca ggggcccgac cgcggctcgc agtaccggag catcgtcctc acccactcgg
     8341 acgagcagcg gacggcggcg gaagcgtccc gggatcgggt ccagtctcgg tacgaacgcc
     8401 cgatcgcgac ggagatccag ccgttcgtgg ccttctatcc ggcggaggac taccaccagc
     8461 gctactacct gaacaacggc cacgagccct attgccactc gatcccgccc ggcctgctcg
     8521 aggacctggg gctcgtccgc acctagcctc ggcgaggcga accctgccaa gctccagggc
     8581 gtgaccgagg catcggcgcg accgaacgat ctccggccgt tcctcgaccg gttcgtcgct
     8641 gcatgcgcgc aggacgaccg gatcgtggcc gcgttcctcg gcggctctcg tggtcgcggt
     8701 gaggcggacg tgtactcgga tgtcgacctc tgcgtgatcg cgagcaacga cgcgtacgag
     8761 gcgctcatcc gcgatcgtgc ggcgttcgtc ggacgcctcg ggacgccgct cttcctcgag
     8821 gacttcgggt tctcggacct cgtgttcttc atcctggacg acggaaccga aggcgagctc
     8881 ttcctcggga gcgaggagcg actggacgag ctcgagccgg gtccgacgat ccggacgctg
     8941 ttcgacccgg gcgggatcct cgaaggcgtc gtgttcgccg aggagcggat ggatcgcaca
     9001 gagcagcggg aagtgctccg gcagatcctc acgtggttct ggcacgagct ctcgcacttc
     9061 atcaccgcga tgggacgggg cgagctctgg tgggccgccg gacaactcga gtcgctccga
     9121 ggtcactgcg tgaacctcgt cagggccgag cacgggtcgt tcgccggcga ggagccgtac
     9181 gagaagctcg accgcacgat cgatctcccc gcgctgtcgc cgatcgccga gacgttcgtg
     9241 ccgatggagc gggacacgct gctgcgagcc gcacgggagc tcgttcgctt ctacacggag
     9301 aaggccccgg gcgtcgcgga ggcgaacggg gcgacgtatc cactcgagct cgagaagctg
     9361 atggtcaagc gacttaacga ccttggcggc tcgcgctagc cgtcgcggaa gccgacgagc
     9421 ttgtgggagc cgtcgcagaa cggcttgttc tgtgagtacc cgcaccggca gagggcctgc
     9481 cggttgcgga cctcgtaggg ctcgccgtcc gcacccacca ccttgacgcc cccgcgcacg
     9541 aacaacggcc cgttcctgaa gacaccgatc tcgggctcgt actcgggctc gtgatcgggc
     9601 gcgtcgaccg agtcggccca cgcgagccgt cccgatggac agagctggac catccggccc
     9661 atgcgttcgc ggacggccgg atcccccagc atctgccaca cgccggtgaa gcggtctttg
     9721 cagtagcccg ccttcgtgca gatcgagaga tcgtcgctga tgatcgctcc gtccggccct
     9781 tggaagacgt cagtcctgtc gtcacgagga ccgtgatccg cgctctccgt gacgtccaca
     9841 cggggttcct cgaggtggct gtcgtcgcag aacggcttgt tcccgctctt gccacagcga
     9901 cagagtcggt aggtctcgtc cgcttccaag ggctcgaagg gtgcgtagtc gaccggttcg
     9961 ccgaactcgg tcttgaccgt cctcgtccgc gcgatcggca gcggaccttt cacctcgtag
    10021 gggccgttct cctcgatccg gatctccggg tccgacatgg gcggcatcct cccacgtcgt
    10081 accatccccg tgaacccagg gagccgccca gg
//
LOCUS       DASUVE010000151         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_453910, whole genome shotgun sequence.
ACCESSION   DASUVE010000151 DASUVE010000000
VERSION     DASUVE010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453910"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VF036_03215"
     CDS             complement(<1..198)
                     /locus_tag="VF036_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6264271.1"
                     /translation="MATDLTVILDDRAGQLAHMGEALGDAGVVIEGISATTSDGVGIV
                     HVLVDNAMVAQNALILADIKVE"
     gene            complement(249..632)
                     /locus_tag="VF036_03220"
     CDS             complement(249..632)
                     /locus_tag="VF036_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6264272.1"
                     /translation="MATDLAVSVADQPGGLADVGEALGNAGINIEGLCGVGLGDRGVI
                     HILVEDGAAARAALEGAGLNVESEAEAIVSAIPGDVRTPGTLGKMARAVAEAGVNMRA
                     VYLATDNRAVAVTDDNAKALAALGM"
     gene            777..1160
                     /locus_tag="VF036_03225"
     CDS             777..1160
                     /locus_tag="VF036_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6264273.1"
                     /translation="MYCPRCGVRAPEESRFCASCGAPLPAGDARSRGPKITRLYLGIQ
                     TSDGDPPDPVLRVSRYLEDHEFSAPEGTTAVPGDHARISIWNIDRPVAAISLTGSDAE
                     ELGRFLSDPEPSVWPRDEAEQDLST"
     gene            complement(1219..2256)
                     /locus_tag="VF036_03230"
     CDS             complement(1219..2256)
                     /locus_tag="VF036_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264274.1"
                     /translation="MVPTGAGLADGTPLPDGCAGAVVASQTVAFVADGRAWALDPDTA
                     ELSCLFPVEDAGPFSWGPQGDRVLLGGFEVRGLGGDAPDLPAIHTAVGAFDWGHPIGL
                     AVAFADGKGNPRKRFIDDGRLIRLDALPPGSYLEVAYHPSGLALSFVVETDEGQEIWL
                     STNEGEDPERLIFSKRGTTFTSLAFSPNGETLWWTAQHAEGYPELHFMHLDTRSGFGT
                     AWRGTEGTVTDGLRLAPAGPRSAVTEGAACEEHEALILDRGETTVALPDEARPTEALG
                     WIDRSTLLVSAGGCGETIDLHAVDARGEEDPTLLALEVEIAAPRTQVIDPPEEVPAPD
                     PEVEPPPDGVG"
     gene            complement(2427..>2593)
                     /locus_tag="VF036_03235"
     CDS             complement(2427..>2593)
                     /locus_tag="VF036_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEX6264275.1"
                     /translation="HDENVARWRSLHVLMVEREIGAKRGTGGSSGVAYLRSTLDKRFF
                     PELWGLRGDL"
BASE COUNT          370 a          967 c          852 g          404 t
ORIGIN      
        1 ctcgaccttg atgtcggcga ggatcagggc gttctgcgcg accatcgcgt tgtcgacgag
       61 cacgtgcacg atcccgacgc cgtcgctcgt cgtcgcgctg atcccctcga tcacgacgcc
      121 agcgtcaccc agcgcctccc ccatgtgcgc gagctggcct gcgcgatcgt ccagaatgac
      181 ggtgaggtcc gtcgccatcc gctcaaccct cccttcgtcc gaacgtttcc ggtcgccctg
      241 ttggagcact acatcccgag agccgcgagc gccttcgcgt tgtcgtccgt tacggcgacg
      301 gcgcggttgt ccgtcgcgag atagacggcc cgcatgttga cccccgcctc ggcgaccgcg
      361 cgcgccatct tccccaacgt ccccggcgtc ctcacgtccc ccgggatcgc gctcacgatc
      421 gcctcggcct cgctctcgac gttgagaccg gcaccttcga gcgcggcgcg agccgcggcc
      481 ccgtcctcga ccaggatatg gatcacccct cgatcgccga ggccgacgcc gcacagccct
      541 tcgatgttga tcccggcgtt gccgagcgcc tcacccacgt cggccaaccc accgggttga
      601 tccgccacgc tgaccgctag atccgtcgcc atcgctggcc ccctctcgcc tttcctcctc
      661 ttgctcgcct tctcttgctc gccctctttg ggggtgtacg cctgcggaca ggcagacgtc
      721 aagccccatg cgccttagag attgccatcg tcggccacca gggcgaggat cggtgcatgt
      781 actgcccgcg atgcggtgtc cgggcgcccg aggagtcccg gttctgtgcc tcgtgcgggg
      841 cgccgctccc ggccggcgac gcgcgatccc gtggcccgaa gatcacgagg ctctacctcg
      901 ggatccagac cagcgacggt gacccgcccg atcccgtcct gcgggtcagc aggtacctgg
      961 aggatcacga gttctccgcg cccgagggaa cgaccgccgt tcccggagac cacgctcgca
     1021 tctcgatctg gaacatcgat cgcccggtcg cggcgatcag tctcaccggg tccgacgccg
     1081 aggagctcgg acggttcttg agcgacccgg agccgtcggt gtggcctcgc gacgaggccg
     1141 agcaggatct ctcgacctga acgcgggtgt ccctgaacct cccatccggc gaccgaccgg
     1201 tcgcccgcga cggcgttatc agccgacgcc gtcggggggc ggctccacct ccgggtcggg
     1261 ggcgggcact tcctccggcg gatcgatcac ctgggttcgc ggggcggcga tctcgacctc
     1321 gagggcgagc agggtgggat cctcctcacc tcgagcgtcc accgcgtgga ggtcgatcgt
     1381 ctcgccgcac ccgccggccg agacgagtaa cgtcgatcgg tcgatccatc ccagcgcctc
     1441 cgtgggccgt gcctcatcgg ggagcgcgac cgtcgtctcc cctcggtcga ggatgagcgc
     1501 ctcgtgctcc tcgcaggccg caccctccgt caccgccgac ctcggtccgg ccggggcgag
     1561 cctcagcccg tcggtgaccg tcccctccgt cccccgccac gccgtgccga atccggatcg
     1621 ggtgtcgagg tgcatgaagt ggagctcggg atagccttcc gcgtgctggg ccgtccacca
     1681 gagggtctct ccgttcggtg agaaggcgag cgaggtgaac gtcgttcccc gcttggagaa
     1741 gatcagccgt tccggatcct cgccctcgtt cgtcgacagc cagatctcct ggccctcgtc
     1801 ggtttccacc acgaacgaga gcgccagacc gctggggtga tacgcgacct cgaggtagct
     1861 cccgggcggc agcgcatcca gcctgatcag gcgcccgtca tcgatgaacc gcttgcgcgg
     1921 gttgcccttg ccgtcggcga aggccacggc cagaccgatc gggtgtcccc agtcgaaggc
     1981 gcccacggcc gtatggatcg cggggaggtc gggcgcgtcg cctcccaacc cacggacctc
     2041 gaacccaccg agcaggaccc gatcgccctg cggtccccac gagaagggac cggcgtcctc
     2101 cacggggaat aggcaggaca gctcggccgt gtcgggatcg agggcccacg cgcgcccgtc
     2161 cgcgacgaac gcgacggtct gggacgcgac gaccgcgcca gcgcatccat cggggagcgg
     2221 cgtgccgtcg gcgagccccg cgccggtggg gaccacggac ggagagtccg atggcgtcgg
     2281 gggaaccgac gggggcggcg agccgtcgtc ggctggcggc tccgtgcagg cgccggccac
     2341 cagcaccgat atgagggcga accggatcca tcgcatcccg ctcccgacgg tacacccggg
     2401 cagtggcacg ggcgagtggc gcccggttag aggtcgcctc gcagtcccca gagctcgggg
     2461 aagaagcgct tgtcgagcgt cgaccggagg taggcaaccc ctgacgaacc gcccgtgccg
     2521 cgcttcgcgc cgatctcacg ctcgaccatc agcacgtgca gcgagcgcca ccgagcgacg
     2581 ttctcgtcgt ggt
//
LOCUS       DASUVE010000152         2583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1583150, whole genome shotgun sequence.
ACCESSION   DASUVE010000152 DASUVE010000000
VERSION     DASUVE010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583150"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..512)
                     /gene="rplI"
                     /locus_tag="VF036_03240"
     CDS             complement(63..512)
                     /gene="rplI"
                     /locus_tag="VF036_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX6264276.1"
                     /translation="MKVILQKTVERLGDPGDVADVADGYARNFLIPRGLAVRAEKGAV
                     RHAESLKRAHQSRTKAQKGEVEAIATRIIQTPVVVTARAGEEGRLFGSVTAADIAEAL
                     SAETGVDVDRRDIHLDEPIRSVGTHEVTVHLHAEVDPVITIDVRAQE"
     gene            complement(523..807)
                     /gene="rpsR"
                     /locus_tag="VF036_03245"
     CDS             complement(523..807)
                     /gene="rpsR"
                     /locus_tag="VF036_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX6264277.1"
                     /translation="MAGRGGPKERGPKRRREKDDKGWQKRGKRKYCQFCKDKVDRVDY
                     KDVMTLRKFVSDRGKIRARRVSGNCVQHQRDVASAVKNAREMALLPYSSR"
     gene            complement(797..1258)
                     /gene="ssb"
                     /locus_tag="VF036_03250"
     CDS             complement(797..1258)
                     /gene="ssb"
                     /locus_tag="VF036_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX6264278.1"
                     /translation="MATDNQVTLVGNLTDDPELRYTPNGAAVCKFRIAVNRRFQDANG
                     QWKDGDTSYFTINAWRTMAENAAESLTRGTRVIVAGRLNYRTWENQDGDKRSAIEVEA
                     DEVAPSLKWATASVTRQARSGGASSSGDWGEKVAAPVGGGTEQEESPLDGG"
     gene            complement(1275..1628)
                     /gene="rpsF"
                     /locus_tag="VF036_03255"
     CDS             complement(1275..1628)
                     /gene="rpsF"
                     /locus_tag="VF036_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX6264279.1"
                     /translation="MREYESMLILPAAADEAVVGTATDRIATVVARHSGAVRNIERWG
                     RRRLAYEIDDQSEGYYVVVRFSAEPAAQTELERTLNLADEILRYKVIVLAPTREQKKK
                     QMASSPASAGGDETP"
     gene            1792..2574
                     /gene="panB"
                     /locus_tag="VF036_03260"
     CDS             1792..2574
                     /gene="panB"
                     /locus_tag="VF036_03260"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX6264280.1"
                     /translation="MTTIHDLKAWKADGQRFTMLTAYDFPTAQILDRAGVPLLLVGDS
                     VGNNVLGYEDTLPVTMEEMLHHTRAVARGAERAMVIGDLPFLSYQVSIEDGVRNAGRL
                     VKEGGAHAVKLEGPHLELVHRLVDIGIPVMAHIGLTPQSVHAMGGYRVQGRGEAAHRV
                     ADEAEQLEKAGAFSIVLEGMPAELAGEITRSLEIPTIGIGAGPSCDAQVLVIHDLLGI
                     NEDVPKLAKKYADVRGVMTDAVRRFVSDVETGVFPDPDHSYS"
BASE COUNT          378 a          902 c          844 g          459 t
ORIGIN      
        1 cgctccgaac gctcgttcgg agaggcgtgt cgtgcggccc gccgcggcca cacgacgggc
       61 gcctactcct gagctcgaac gtcgatcgtg atgacggggt cgacctcggc gtgcagatgg
      121 acggtgacct cgtgcgtccc cacggagcgg atcggctcgt cgagatggat gtcgcgtcgg
      181 tcgacgtcaa caccggtctc ggccgagagc gcctccgcga tgtcggcggc cgtgaccgat
      241 ccgaacagcc tgccctcctc gcccgcccgc gccgtgacca cgacaggggt ctggatgatg
      301 cgagtggcga tcgcctcgac ctcgcccttc tgcgccttcg tccgcgactg atgggcgcgc
      361 ttgaggctct ccgcgtgacg aacggcccct ttctcggctc gcacggcgag gccgcgtggg
      421 atcaggaagt tccgcgcgta tccgtcggcg acgtccgcga cgtcaccggg atcgccgagg
      481 cgctcgaccg tcttctgcag gatgactttc atcgcggcgg tcctatcggc tcgagtacgg
      541 cagcaacgcc atctcgcgag cgttcttcac cgcggacgcc acgtcgcgct ggtgctgcac
      601 gcagttcccc gagacgcgcc gtgcgcggat cttgcctcgg tccgacacga acttgcggag
      661 cgtcatcaca tccttgtagt cgacgcgatc gaccttgtcc ttgcagaact ggcagtactt
      721 ccgcttgccg cgcttctgcc agcctttgtc gtccttctcg cgacgccgct tgggaccgcg
      781 ctccttcggt cctcccctac ccgccatcga ggggagactc ctcttgttcg gtcccgccgc
      841 cgaccggtgc ggcgaccttc tcgccccagt cgccggagct cgacgccccg ccggagcgag
      901 cctggcgtgt cacggatgcc gtcgcccact tcaggctcgg ggcgacctcg tcggcctcga
      961 cctcgatcgc cgagcgcttg tcgccgtcct ggttctccca ggtccggtag ttgagccggc
     1021 ccgcgacgat cactcgcgtg ccgcgcgtga gggactccgc cgcgttctcc gccatcgtcc
     1081 gccatgcgtt gatcgtgaag tacgacgtgt cgccgtcctt ccactggccg ttcgcgtctt
     1141 ggaaccgacg gttgacggcg atccggaact tgcacaccgc agccccgttc ggggtgtagc
     1201 gcagctctgg gtcgtcggtc aggttgccga ccagggtcac ttgattgtcc gtcgccatgg
     1261 ttcgttcact ccccttaggg tgtctcgtct ccgccagcgg aggcggggga agatgccatc
     1321 tgcttcttct tctgctcacg tgttggggcc aggacgatca ccttgtaccg caggatctcg
     1381 tccgcgaggt tcagcgtccg ctcgagctca gtctgggctg caggctccgc cgagaacctg
     1441 accacgacgt agtagccctc gctctggtcg tcgatctcgt aggccaggcg acgccggccc
     1501 cagcgctcga tgttgcgcac cgctccggaa tgcctcgcga cgaccgttgc gatccggtcg
     1561 gtcgcggtac ccaccacggc ttcgtcggcc gcggcgggca ggatgagcat gctctcgtac
     1621 tccctcaaac cccacctcct ctggtctgga cggcccccct cgaaggggga gcaggaagtc
     1681 ctgagggtcg tccagcagtg tacgggacct gcgctgacgg tagcaccgag gtacgacatc
     1741 gggtgggcta gaatcgctcg actgccgtgc acttcagagg aggcgcgagc gatgaccacg
     1801 atccacgacc tcaaggcgtg gaaggcggac gggcagaggt tcacgatgct caccgcctac
     1861 gacttcccca ccgcccagat cctcgatcga gccggggtcc cgctgctgtt ggtgggcgac
     1921 tccgtgggga acaacgtcct cggctacgag gacaccctcc cggtcacgat ggaggagatg
     1981 ctccaccaca cccgggccgt cgcgaggggt gccgagcggg cgatggtgat cggagacctg
     2041 cccttcctct cctaccaggt ctcgatcgag gatggggtgc gcaacgccgg ccgcctcgtg
     2101 aaggagggcg gcgcgcacgc cgtgaagctc gaggggccgc acctcgagct cgtccatcga
     2161 ctggtcgaca tcggcatccc ggtcatggcg catatcggcc tcacgccgca gtcggtccat
     2221 gccatgggcg ggtatcgggt gcagggccgg ggcgaagcgg cgcaccgcgt cgcggatgag
     2281 gccgagcagc tcgagaaggc aggcgcgttc tcgatcgtcc tggaaggcat gccggccgag
     2341 ctcgccggcg agatcacccg atcgctggag atccccacga tcgggatcgg cgcaggtccg
     2401 tcctgcgacg cacaggtgct cgtgatccac gacctcctgg ggatcaacga ggacgtcccg
     2461 aagctcgcga agaagtacgc ggacgtccga ggcgtgatga ccgacgccgt caggaggttc
     2521 gtaagcgacg tcgagaccgg cgtcttcccg gacccggacc actcctactc ctagacgcct
     2581 ccc
//
LOCUS       DASUVE010000153         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1395407, whole genome shotgun sequence.
ACCESSION   DASUVE010000153 DASUVE010000000
VERSION     DASUVE010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395407"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VF036_03265"
     CDS             complement(<1..550)
                     /locus_tag="VF036_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020512344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEX6264281.1"
                     /translation="MAFHGWKVEALEFFEGLEADNSKTYWQGHKDVYDTIVRAPMEEL
                     LAELEPEFGEGRIFRPYRDVRFSRDKSPYKTAIGATLGQGYVHVDARGLAVGSGMWEM
                     APDQLERYRRAVADDGSGEELDRIVTAARAEGLDVHGHDVLKTAPKGYPKDHPRIELL
                     RCKGLIAWQEWPAGAWLGTKRAK"
     gene            complement(584..1555)
                     /locus_tag="VF036_03270"
     CDS             complement(584..1555)
                     /locus_tag="VF036_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6264282.1"
                     /translation="MRRSSLSLRLPAAVIALAAATGLLAPGAGATDHDPVIVAVGDLA
                     CQSLTAGQGMGACRSGDVADLIREIDPDRFLALGDLQYSNGKLSEFLRVWDVQFGDLR
                     GITMPAPGNHEYGTPDAAGYFAYFGAVANPPLGYYSFDLGDWHIVSLNSDICGDEPGC
                     GPGTPQGDWLRADLEAHAEATCTLAFMHHPRYDWRPFQKWIEDDATTNFGGSETRPLV
                     PLWEAFVDEGVDVVLVGHNHLYQRWAPQDAHGEAVDGGTVQFTVGTGGRQLYPFGRPP
                     RPENLEATQNKAFGVLQMTLHEDSYDYRWVSVPGERTFRDGGTVACT"
     gene            complement(1634..1942)
                     /locus_tag="VF036_03275"
     CDS             complement(1634..1942)
                     /locus_tag="VF036_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264283.1"
                     /translation="MDDCQLCVAEQVTAWLHEDDDCWIAECLVCRTPMIVWRVHGLPE
                     AELESDLLIRLERIATDRYGPDGYWIDPERRRIPEHWHAHARPTGGFFDPTSDLYGAF
                     "
     gene            complement(1935..2399)
                     /locus_tag="VF036_03280"
     CDS             complement(1935..2399)
                     /locus_tag="VF036_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX6264284.1"
                     /translation="MRQLIGTLITLTLLGACTDDTSGPSESGPASTMTFGGTDATLAV
                     DLADDPQERRKGLMGVEELPADQGMAFVWPGPVDSTFWMKDTLVPLSVAFVDESGYVI
                     DVLDMQPCETDPCPSYGIDEPFVLAVEANLGWFDEHGVGAGDPAELRGTLGG"
BASE COUNT          369 a          858 c          819 g          407 t
ORIGIN      
        1 ccttcgctcg cttcgttcca agccaggcgc cggccggcca ctcttgccac gcgatcaggc
       61 ccttgcaccg caggagctcg attcgtggat gatccttcgg ataccccttc ggggcggtct
      121 tcaggacgtc gtgcccgtgg acgtcgaggc cctcggcgcg cgctgccgtc acgatccgat
      181 ccagctcctc acccgatccg tcgtcggcga ccgcccggcg gtagcgctca agctggtcgg
      241 gcgccatctc ccacatcccg ctcccgacgg cgagaccgcg cgcgtccacg tggacgtagc
      301 cctgcccgag cgtcgcgccg atcgcggtct tgtagggcga cttgtcgcgg ctgaagcgga
      361 cgtcgcgata cgggcggaag atccggcctt cgccgaactc gggctccagc tcggcgagca
      421 gctcctccat cggtgcgcgc acgatcgtgt cgtacacgtc cttgtgtccc tgccagtacg
      481 tcttcgagtt gtcggcctcc agaccctcga agaactcgag cgcctccacc ttccatccgt
      541 ggaacgccat cggatccgac ctcctccacc ccgctcgccc ggatcaggtg caggctaccg
      601 tccctccgtc ccggaacgtc cgctcgccgg gcacggagac ccatcggtag tcgtacgagt
      661 cctcgtgcag ggtcatctgc aacaccccga acgccttgtt ctgggtcgcc tcgaggttct
      721 cgggcctcgg agggcgcccg aacgggtaga gctgccgacc gcccgttccc accgtgaact
      781 ggaccgttcc gccgtcgacg gcttcgccat gggcgtcctg tggagcccac cgctggtaga
      841 gatggttgtg cccgacgagc acgacgtcga cgccttcgtc cacgaatgct tcccagagcg
      901 ggacgagcgg tctggtttcc gagccgccga agttggtcgt ggcgtcgtcc tcgatccact
      961 tctggaaggg ccgccagtcg tagcgagggt gatgcatgaa cgcgagcgtg caggtggcct
     1021 ccgcgtgcgc ctcgaggtcc gcccggagcc agtcgccctg gggtgtgccg gggccgcagc
     1081 cgggctcgtc gccgcagatg tcggagttca gcgacacgat gtgccagtcg ccgaggtcga
     1141 aggagtagta gccgagcgga gggttggcca ccgcgccgaa gtacgcgaag tagccggcgg
     1201 cgtcgggcgt cccgtactcg tggttccccg gggccggcat ggtgatcccc cggaggtcgc
     1261 cgaactggac gtcccagacg cggaggaact ccgagagctt gccgttgctg tactggaggt
     1321 cgccgagcgc gaggaaccgg tcggggtcga tctcgcggat caggtccgcg acgtctcccg
     1381 atcggcaggc acccatccct tgccccgccg tcagcgattg gcacgcgagg tcgccgaccg
     1441 cgacgatcac gggatcgtga tccgtcgcac cggctcccgg tgcgagcaga ccggtcgccg
     1501 cggccagcgc gatcacggct gcggggaggc ggagcgaaag agacgagcgg cgcatctgat
     1561 ctccttccgg gcgggggcgc gtattcaagc atgcaaggac ggcgaaggga atggctccgg
     1621 ggtaccagcg gggtcagaac gcgccgtaga ggtcgctcgt ggggtcgaag aacccacctg
     1681 tcgggcgggc gtgtgcgtgc cagtgctccg ggatccggcg tcgctcgggg tcgatccagt
     1741 agccgtccgg cccgtaccgg tcggtggcga tgcgctcgag ccggatcaac aggtccgact
     1801 cgagctcggc ctcaggcaac ccgtgcacgc gccacacgat catcggcgtt cggcacacga
     1861 ggcactccgc gatccagcaa tcgtcgtcct cgtggagcca ggcggtgacc tgttccgcga
     1921 cgcagagctg gcagtcatcc accgagggtc ccccggagct ccgctggatc gccggcaccg
     1981 acgccgtgct cgtcgaacca gcctaggttc gcttcgaccg cgaggacgaa cggttcgtcg
     2041 atcccgtacg aggggcacgg gtccgtctcg cacggctgca tgtccaggac gtcgatcacg
     2101 tacccggact cgtcgacgaa ggcgaccgag agaggcacga gcgtgtcctt catccagaac
     2161 gtgctgtcga cgggccctgg ccacacgaat gccatcccct gatcggccgg gagctcctcg
     2221 acgcccatca atcccttccg gcgttcctgc ggatcgtctg cgaggtccac cgcgagcgtc
     2281 gcgtccgttc ctccgaacgt catcgtcgac gccgggccgg actccgaggg tcctgatgtg
     2341 tcgtcggtgc acgcccccaa gagggtcagg gtgatcaggg tcccgatgag ctgccgcacc
     2401 acttcatcgt gccacggtcg gccgccaacc cattgttatc atcgggcact gcc
//
LOCUS       DASUVE010000154         5419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1584460, whole genome shotgun sequence.
ACCESSION   DASUVE010000154 DASUVE010000000
VERSION     DASUVE010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584460"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1734
                     /locus_tag="VF036_03285"
     CDS             <1..1734
                     /locus_tag="VF036_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEX6264285.1"
                     /translation="PRPYGVTIIGGWGGFALPSAPVAALTAVLIGIAYSLVVPGRPRW
                     FAKLAIAFFIGLFVAARMYLGVDGPPGVIYGLILGVAFPLTAFRWFTPNEVFPVVYRK
                     GSTAHLDVTGPRGEAIREAVQEQLGLTVLEIKPVGLEGSGGSTPLRLKVAGDPDTYLF
                     AKLYAKSHVRADRWYKLSRIVLYGALEDEASFQSVRRFVEYEDYTLRLMTDEGIPVPT
                     PQGIVEISPEREYMIVMEFFEGAREIGEVDVDEGIVDEGLSLIRKLWDAGLAHRDIKP
                     ANLMVRDGKVLLIDVFFAQVKPSPWRQAVDLGNMMLVLAVRSDVDLVYRRALTFFTAD
                     EIAEAFAATRGIASPTQLRAFMKRDPRDLVGQFRALAPERRPISIQRWSIRRVLLALG
                     MLIAFAIAVSSSINAFLPAQNLEIARAPECGVSRASILSAQSVPTAATIPCLDALPSG
                     WTYGGGDIHSGEASFWLNSDLGGMRAVTVTLRSACDVSSAHEVPSDEAGTRRFEAPSS
                     LAPQLLSVRSYVFPGGCATYRFAFAEGASTAMVFDVDEAVSFVPRADLVAHLRETEGL
                     ALCGRGAPCPP"
     gene            1731..2438
                     /locus_tag="VF036_03290"
     CDS             1731..2438
                     /locus_tag="VF036_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6264286.1"
                     /translation="MSGHAVGTRDPGDDLEHPLLGMPRRDTAVTAVLAATTVAFFALM
                     ATDAGRRLIQPNDDAFLRWIVSLRIGWLTVVAHVFNLIGLTYVMAPIRLGIAGWLAIR
                     RRWWHFAAFVSAILVSEVAIGTLKALYDRPRALGSLVETSNASFPSGHAVAASVTAVA
                     AVIALVPEGPRRYRWGAAAVGFSFLMGLSRTYLGAHWLSDAIAGVLLGTTIALGTALI
                     VHRIREGRTSGEVTPLG"
     gene            2623..3561
                     /locus_tag="VF036_03295"
     CDS             2623..3561
                     /locus_tag="VF036_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6264287.1"
                     /translation="MTATLVCSGLRKSFGDRVAVDGVEFSIAAGETYGLLGPNGAGKT
                     TTISMVCGLLTRDAGEILLEGRPFDTTSIDAKASIGLVPQEIAIYPDLSANENLAFFG
                     RLYGLHDPELSDRVAEVLETVGLQDRANDRTSEFSGGMKRRLNIGLGLLHRPQLLVLD
                     EPTVGVDPQSRNAILDSVEQLGAGGLSVLYTTHYMEEAERLCDRVGILDEGRLLAEGT
                     RRELVSSIGAHDRVRMIAPGISEPVLVGLRGIPGVVEVSARDAQVDLLVDDARSALGP
                     IVQTVTSDGGTVSSVEVQEPDLEAVFLSLTGKALRD"
     gene            3566..4735
                     /locus_tag="VF036_03300"
     CDS             3566..4735
                     /locus_tag="VF036_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6264288.1"
                     /translation="MGTSQGRASRTIAEKDLRQRMRDRTALLVGVLLPFALASILALT
                     LGGAAEGDLDLDIGLVDLDRGAVADRFEETLRAADVGTVHPLGDRDVAERRAEEGALD
                     AAFVLPVDLSARSTSGGGGSIEVIGSPDSDLGPLVATSIARSFAARLDGIAIAVATAS
                     ETPEDGPSIADAAARLPPAATLDISVTADEGVTPSTFAAIGMAVFFLFFTVEFGIRSV
                     IEEREQGTLARLLAAPIRPASVLIGKGLASFVVGVLSTSLLVVATTLLLDASWGPLIG
                     VALLVLTGVGTAVAVTSFVATFATTSTQAGNYASIVAVVGGLLGGTFFPISQAPGLLA
                     SARFLSPQGWLMEGFTELSTGGGLVGALPALAGTLVIGVAFGTLAWVRARRMLSP"
     gene            4732..>5419
                     /locus_tag="VF036_03305"
     CDS             4732..>5419
                     /locus_tag="VF036_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6264289.1"
                     /translation="MTSARKALAIAAVNVRRLTKDRTGLFFVVIFPFLLIFVLGATFG
                     SGFTPRVGVVAAEAPLAGEIVQRLRSVDGIEVRTFVSADALGEAVLLGDVQSGFVIPR
                     SYDADIRDGRPVEIPWVSLPTGAGGDLSLIVASVIDQQSSLVNAAHLAQERGAADSFD
                     EALAKARAASEAIPIVAVRTSTAGNPEGSGLSTAAAQELTLFVFVTSLSAASMLVESR
                     RLGVSRRMIAS"
BASE COUNT          791 a         1801 c         1848 g          979 t
ORIGIN      
        1 cctcgtccat atggcgtgac gatcatcggt ggatggggcg ggttcgcgtt gccgtcggcg
       61 cccgtcgccg cgctcactgc ggtcctgatc ggcatcgcct attccctggt cgttcccggg
      121 cgcccccggt ggttcgcgaa gctcgcgatc gccttcttca tcggcctctt cgtggccgcg
      181 aggatgtatc tcggggtcga cggaccgccg ggcgtgatct acggcctgat cctcggcgtg
      241 gcgttcccgc tgacggcgtt ccgctggttc accccgaacg aggtcttccc cgtcgtctat
      301 cggaagggca gcactgcgca cctcgacgtg accggtccac gtggagaggc gatccgggag
      361 gcggtgcagg agcagctcgg gctcacggtg ctcgagatca agccggtcgg gctggaggga
      421 tcgggaggtt ccacaccgct tcgcctcaag gtcgccggcg atccggacac ctacctgttt
      481 gcgaagctct acgcgaagag ccatgtacgc gcggatcgct ggtacaagct gtcgcggatc
      541 gtcctctacg gcgcactcga agacgaggcc tcgttccagt ccgtccggcg tttcgtcgag
      601 tacgaggact acacgcttcg acttatgacg gatgaaggca tccccgttcc gacaccgcag
      661 gggatcgtcg agatctcgcc cgaacgcgaa tacatgatcg tcatggagtt cttcgagggt
      721 gctcgcgaga tcggcgaggt cgacgtcgac gaggggatcg tggatgaggg gctgtcgctg
      781 atccgcaagc tgtgggacgc gggtctggcg catcgcgata tcaagcccgc gaacctgatg
      841 gtgagggacg ggaaggtgct cctgatcgat gtcttcttcg cgcaggtgaa gccgtcgccc
      901 tggcggcagg cggtcgacct cggcaacatg atgctcgtcc tcgcggtccg ctcggacgtg
      961 gacctggtgt accggcgcgc actcacgttc ttcacggccg acgagatcgc cgaggcgttc
     1021 gctgccaccc gtgggatcgc cagtcccacg cagctccggg ccttcatgaa gcgggacccc
     1081 cgcgacctcg tggggcagtt ccgggcgctc gcgcccgagc gccggccgat ctcgatccaa
     1141 cgatggagca ttcgacgggt cctcctcgcg ctcgggatgc tcatcgcgtt cgcgatcgcg
     1201 gtctcttcca gcatcaacgc cttccttcca gcacagaacc tggagatcgc gagggcgcct
     1261 gagtgcgggg tgagccgtgc gtccatcctc tcggcacagt ccgttccgac tgcggctacg
     1321 atcccctgtc tcgacgctct tccctccggg tggacctatg gaggcggcga catccacagc
     1381 ggcgaggcga gcttctggct gaactccgat ctggggggga tgcgagcggt cacggtcacg
     1441 cttaggtccg cgtgcgacgt gtcgagcgcc cacgaggtcc cttccgatga agccggcacg
     1501 cgcaggttcg aggctccgtc gagcctcgcg ccgcagctcc tcagcgttcg ctcctacgtg
     1561 ttcccggggg gatgcgccac gtatcggttc gccttcgctg aaggtgcctc cacggcgatg
     1621 gtgttcgacg tggacgaggc cgtgtcgttc gtccccagag ccgacctggt ggcgcacctc
     1681 cgagagacgg agggtctcgc cctctgcggc cggggagcgc cgtgtccgcc gtgagcggcc
     1741 acgcggtcgg aacaagggat ccaggggacg acctggagca cccgctcctc gggatgccgc
     1801 gacgcgacac ggcggtcacg gcggttctcg cggcaacgac cgtggccttc ttcgctctca
     1861 tggccaccga tgccgggcgc agactgatcc aaccgaacga cgacgcgttc cttcgatgga
     1921 tcgtgtcgct ccggatcgga tggctgacgg tggtcgctca tgtcttcaac ctgatcgggc
     1981 tgacatacgt gatggcaccg atacggctcg ggatcgccgg atggttggcg atccggcgga
     2041 ggtggtggca cttcgccgcg ttcgtctccg cgatcctcgt ctcggaggtc gcgatcggga
     2101 cgctcaaggc gctgtacgac cggccgcggg cgctcggatc gctcgtcgag acgagcaacg
     2161 cctcgttccc ctcagggcac gccgtggccg cctccgtcac cgccgtggca gcggtgatcg
     2221 cgctggtccc cgaagggccg cgccgctatc ggtggggggc ggcggccgtc gggttctcgt
     2281 tcctgatggg cctctctcgg acctacctgg gagcgcactg gctctccgac gcgatcgcgg
     2341 gtgtgctgct cggtaccacg atcgcgctgg ggaccgccct gatcgtccac cggatccgcg
     2401 aaggtcgaac gtcaggtgaa gtcacgccgc tgggatagca cccttcccac cacgatcaac
     2461 ggcgcggtcc acgtcaggaa gagcgcggca cccacgcccg gattcgaggg gcgggccttc
     2521 catggtgacc gcgtagcgtt gcgcgccggc ggtgaacggc aaagtacgtc gcgaggttgg
     2581 gcaggaacga cccccaaagc ttcgtccgct aggctgcggc gaatgaccgc gacgctggtg
     2641 tgcagcgggc tccgcaagtc gttcggtgac cgagttgcgg tcgacggggt ggagttctcc
     2701 atcgccgccg gcgagaccta tggattgcta gggcccaacg gcgccggcaa gaccacgacc
     2761 atctcgatgg tgtgcggctt gctgacacga gacgccgggg agatcttgct cgaaggccgg
     2821 cctttcgata cgacgagcat cgatgcgaag gcctccatcg gcctcgtccc ccaggagatc
     2881 gcgatctatc cggatctgtc ggcgaacgag aacctcgcgt tcttcggccg actgtacggg
     2941 cttcacgatc ccgagctcag cgatcgggtc gcagaggttc tcgagacggt cgggcttcag
     3001 gaccgggcca acgatcgaac atcggagttc tcgggtggga tgaagcgacg gctgaacatc
     3061 gggctgggac tcctccatcg cccgcagctc ctcgtcttgg acgagccgac cgtcggcgtg
     3121 gacccgcaga gtcgcaatgc catcctcgac tccgtcgagc agctcggcgc gggtggcctg
     3181 tcggtcctgt acacgacgca ttacatggag gaggcggaac gcctctgcga ccgtgtgggg
     3241 atcctcgacg aggggcgact gctcgccgag ggcacccggc gagagctcgt ctcgagcatc
     3301 ggggcgcacg atcgcgtccg gatgatcgct ccggggatct ccgagccagt cctcgtcggc
     3361 ctgcgcggca ttcccggcgt ggtcgaggtt tctgcacgag atgcccaggt cgaccttttg
     3421 gtcgatgacg ccaggagtgc actcgggccg atcgttcaga ccgtgaccag cgatggcggg
     3481 accgtgtcct ccgtggaggt tcaggagccc gatctcgagg cggtgttcct gtcgctcacg
     3541 ggcaaggcgc ttcgcgacta gcccgatggg cacatcccaa ggccgagcat ctcggacgat
     3601 cgcggagaag gatcttcggc agcggatgcg agaccggacc gccctgttgg tgggcgtgct
     3661 cctcccgttc gcgctggcct cgatcctcgc cctcacgctc ggcggcgccg ccgaaggcga
     3721 cctcgatctc gacatcgggc tcgttgatct cgaccgaggg gccgtcgcag accggttcga
     3781 ggagacgctc cgagcagcgg acgtcggaac cgtgcaccct ctcggcgatc gagatgtcgc
     3841 agaacgacga gccgaggaag gcgccctcga cgccgcgttc gtccttccgg ttgatctctc
     3901 ggctcggtcc acatccggag gcggagggtc gatcgaagtg atcggctcgc ccgactccga
     3961 tctcgggccc ctggtcgcca cctcgatcgc ccgctccttc gccgcgcgac tggacggcat
     4021 cgcgatcgcc gttgcgacgg caagcgagac acccgaggac gggccgtcca tcgccgatgc
     4081 ggcggcgcgg ttgccgccag ctgccactct ggacatcagc gtgaccgccg acgaaggcgt
     4141 cactccgtcg accttcgcgg cgatcggcat ggcggtgttc ttcctcttct tcacggtcga
     4201 gttcggcatc cgcagcgtga tcgaagagcg tgagcagggc acactcgcgc gactgctcgc
     4261 cgcccccatc cgtcctgcct ctgtgctgat cggcaagggt ctcgccagct tcgtggtggg
     4321 cgttctctcg accagcctgc tcgtggtggc caccacactg ttgctcgatg cgtcgtgggg
     4381 tccgttgatc ggcgtcgcgc tgctggtcct gacgggcgtg gggacggccg tggcggtcac
     4441 cagcttcgtc gcgaccttcg ccacgacatc gacccaagcc ggcaactatg cgtcgatcgt
     4501 tgcggtcgtc ggcgggctcc tcggaggaac cttctttccg atctcacagg cacctggact
     4561 cctcgcgtcc gctcgattcc tctccccgca ggggtggctg atggaaggat tcaccgagct
     4621 ctcgaccggc ggcggactcg tcggtgcact ccctgccctg gccggcacgc tcgtgatcgg
     4681 agtggcgttc ggaaccctcg cgtgggttcg agcacgaagg atgctgagcc catgacctcc
     4741 gcccgcaagg cgctcgcgat cgcggcggtc aacgttcggc ggctgacgaa ggaccgcacc
     4801 ggcctgttct tcgtggtgat cttcccgttc ctgctgatct tcgtcctcgg agccaccttc
     4861 ggctcgggat tcacaccgcg cgtgggcgtc gtggcggcgg aggcgcccct cgctggcgag
     4921 atcgttcagc gtctgcgctc ggtggacggg atcgaggtcc ggacgttcgt gagcgcagat
     4981 gccttgggtg aggcggtcct gctcggcgat gtccaaagcg gcttcgtgat cccccgcagc
     5041 tacgacgccg acatccgtga cggacgaccg gtcgagatcc cgtgggtgtc tctgcccaca
     5101 ggtgcgggcg gtgacctgtc gctcatcgtc gcgtcggtga tcgatcagca gagctcgctc
     5161 gtgaacgcgg cccacctggc gcaggagcgc ggggcagcgg actcgttcga cgaggcgctg
     5221 gcgaaagcgc gcgcggcgag cgaagcgatc ccgatagtcg cggtccgtac atcgacggca
     5281 gggaacccgg agggcagcgg actgagcacg gcagccgccc aggaactgac gctcttcgtg
     5341 ttcgtgacct ccctgtcggc tgcgtcgatg ctcgtcgaat cgcgccgact cggcgtctcc
     5401 cgtcggatga tcgcctccc
//
LOCUS       DASUVE010000155         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_266395, whole genome shotgun sequence.
ACCESSION   DASUVE010000155 DASUVE010000000
VERSION     DASUVE010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266395"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1616
                     /locus_tag="VF036_03310"
     CDS             <1..1616
                     /locus_tag="VF036_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264290.1"
                     /translation="ASRAADAARGAVGHPSMGIAGLLPIAGENIDATAAVADASQATA
                     EAGTEMVRVARTLGWTDIRVPASTSVGNVDIDALEDAIPDMEAVTRQLDVALRALEDE
                     GSDGLLGPVASGYQDAVDSLTLRLDLAIRFRDSLRLAAAMFGGEHRYLVCVPTLGIPR
                     PAGGTPATVGVLIARDGSLALESIAPASSELIEAETSMDWRRTARAFLEAAEASGLTD
                     LDGVIQIDAVALEDIVWAIGDVGSDGSSEALSDRTTIRALEIEAFLGTAPPRSARLHA
                     DRVSAILEAFLDRRPGVESFALATAAGARDGHLSIYLPGKAERRIIHSLGLDGGARLT
                     DDGVLPVAASWTAVGNSHVGAFVDTTVRQSIRILGDGSAAVAAEILFENEAGTGPPSV
                     LLGRPVGGIPVGTFAADVTLFVPATAEDITAETSRPSPIEIGSDMGLTSVTGSILVRG
                     GESATLTVTYDVPDAVTTVDGLEQLTLRVLPQPTLSGVRFQLGIILPEGSNVVSTSRE
                     LERRTDGATFSGVRGGSVDLELRFAAGEP"
     gene            complement(1613..2029)
                     /locus_tag="VF036_03315"
     CDS             complement(1613..2029)
                     /locus_tag="VF036_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6264291.1"
                     /translation="MRKSDGKHFGQRLRESRVAAGLSQSELEDISGIPKARLSRYENG
                     HVEPSIQTLARLSRALNVSEASLLGDQRAILEDFFNVLYDRGVRITSSEQGARIAHAI
                     ADLLEAVKTDPAVAEHGQMTRLPDAVEHPVAAPMLG"
     gene            complement(2140..>2515)
                     /gene="rplL"
                     /locus_tag="VF036_03320"
     CDS             complement(2140..>2515)
                     /gene="rplL"
                     /locus_tag="VF036_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX6264292.1"
                     /translation="EDLLEQFKEMTLLELSEFIKAFEETFDVTAAAPAPMMVAAPGGA
                     AAGDGGAEEEQSEFDVILTAAGDKKIQVIKEVRALTSLGLKEAKDLVDGAPKPVLERV
                     NKEAADKAKEQLEAAGGSVEVK"
BASE COUNT          357 a          873 c          864 g          421 t
ORIGIN      
        1 ccgccagccg ggcggcggat gccgcccggg gggcggtggg gcacccctcg atgggcatcg
       61 ccggtctcct gcccatcgcg ggcgaaaaca tcgacgcgac ggcggccgtc gccgacgcct
      121 cccaggcgac ggcagaggcc gggacagaga tggtccgggt cgctcggacg ctcgggtgga
      181 ccgacatcag ggtccccgcc tccacctccg tggggaacgt ggacatcgac gccttggagg
      241 acgcgatccc cgacatggag gcggtaacgc gacagctcga cgtcgctctc cgggcgctgg
      301 aggacgaggg gagcgacgga ctgctcgggc ccgtcgcctc cggctatcag gacgccgtcg
      361 actcgctcac cctccggctc gacctcgcga tccgattcag ggactccttg cggctcgcgg
      421 ccgcgatgtt cgggggggag catcggtacc tcgtctgcgt cccgacgctg ggcatcccac
      481 ggccggccgg cggcacgccc gcgacggtcg gcgtgctgat cgcacgcgac ggctccctcg
      541 cactcgagtc gatcgcgccc gcatcgagcg agttgatcga ggcggagacc tcgatggact
      601 ggaggaggac ggcgcgagcc tttctcgagg cggccgaagc gtccggtctg accgatctcg
      661 acggggtcat ccagatcgat gcggtggcac tcgaggacat cgtgtgggcc atcggggacg
      721 tgggatcgga tggttcgtcc gaggccctgt ccgaccggac caccatcaga gctcttgaga
      781 tcgaggcctt cctcgggacc gcaccgccca ggtcggcccg gctgcacgcg gatcgggtct
      841 cggcgatcct cgaggcgttc ctcgatcgtc ggccaggcgt ggaatcgttc gccctggcga
      901 cggccgccgg cgcccgcgac gggcacctgt cgatctacct ccctgggaag gccgagcgtc
      961 gcatcatcca cagcctcggc ctcgacggcg gcgccaggct gaccgatgac ggcgtgctcc
     1021 ccgttgccgc ctcctggacc gcggtcggga actcccacgt cggtgcgttc gtggacacga
     1081 ccgtccgtca gtcgatccgg atcctgggcg acgggtccgc tgcggtcgcc gcggagatcc
     1141 tgttcgagaa cgaggcggga acgggtccgc cgtcggtact cctcgggcgc cccgtgggcg
     1201 ggataccggt gggcacgttc gcagcggatg tcaccctctt cgtgccggcg acggcggagg
     1261 acatcaccgc ggagacgagt cggccgagcc cgatcgagat cggctcggac atgggactca
     1321 cctcggtgac cggttcgatc ctcgttcgcg gtggcgagtc ggcgacgctc accgtcacgt
     1381 acgacgtccc tgacgcggtc acgacggtcg acggactgga gcagctgacg ctcagggtcc
     1441 tccctcagcc gacgctgtcc ggcgtgcggt tccagctcgg gatcatcctc ccggaggggt
     1501 cgaacgtcgt ctccacctca cgggagctcg agaggaggac ggacggggcc acgttctccg
     1561 gcgtccgcgg aggctccgta gacctcgagc tgcgattcgc tgccggcgag ccctagccga
     1621 gcatcggggc ggcgacgggg tgctcgaccg cgtccggcaa ccgcgtcatc tgtccgtgtt
     1681 ccgcgacggc cggatcggtc ttcacggctt cgagcaggtc agcgatcgcg tgggcgatcc
     1741 tcgcgccctg ctcgctggag gtgatgcgca cgccgcggtc gtagaggacg ttgaagaagt
     1801 cttcgaggat cgcccgctga tcgccgagca gtgaggcctc ggacacgttc agggcacgcg
     1861 acaaccgggc gagggtctgg atggaaggct cgacatgacc gttctcgtag cgtgagagtc
     1921 gcgccttggg gatgccggag atgtcctcga gctccgattg gctcagcccc gctgccaccc
     1981 gtgactctcg gagtcgctga ccgaaatgct tgccatcgct cttccgcatg ccctgtctcc
     2041 ccctcatgtg agaccgttcc gcacgctagc acaagggttg acgggagggg gaacccgggg
     2101 tcccgccgca gcgtcacccc aggttgctcg tcgggttctc tacttgacct cgacgctccc
     2161 gcccgcggcc tcgagctgct ccttcgcctt gtcggcggcc tctttgttga cacgctcgag
     2221 gaccggcttg ggcgctccgt cgacgagatc cttggcctcc ttgagaccca ggctcgtgag
     2281 agcccggacc tccttgatca cctggatctt cttgtcccct gcggcggtga ggatcacgtc
     2341 gaactccgat tgctcctctt ccgcgccacc gtcaccggcg gctgcaccgc ccggcgcggc
     2401 caccatcatc ggcgcaggtg cagcggccgt gacgtcgaac gtctcttcga acgccttgat
     2461 gaactccgac agctcgagca gcgtcatctc tttgaactgc tcgaggagat cctcc
//
LOCUS       DASUVE010000156         4093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1397235, whole genome shotgun sequence.
ACCESSION   DASUVE010000156 DASUVE010000000
VERSION     DASUVE010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397235"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..533
                     /locus_tag="VF036_03325"
     CDS             162..533
                     /locus_tag="VF036_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6264293.1"
                     /translation="MELGAFSVSLAVRDLGASRTFYESLGFQVVMGDASENWLILRNG
                     DTTIGLFHGMFERNVLTFNPGWDRDARPLTVFTDVRELQREIRERGVAIEQPADESST
                     GPASFIVVDPEGNPILFDQHV"
     gene            complement(649..1047)
                     /locus_tag="VF036_03330"
     CDS             complement(649..1047)
                     /locus_tag="VF036_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6264294.1"
                     /translation="MFEKIVLAVDGSEHSRKAAEVTAGLGRDGAEVLVVHVHEVGVVA
                     SIETPSDAQSLVDGVVRELNDAGLKATGSAVTARAGSTAPALLDAAKAFDADVIVMGT
                     RGLSDFAGLLLGSIAHKVIHHAECPVLVVR"
     gene            1309..3774
                     /gene="gltA"
                     /locus_tag="VF036_03335"
     CDS             1309..3774
                     /gene="gltA"
                     /locus_tag="VF036_03335"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019190155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HEX6264295.1"
                     /translation="MDGRNGRFGRSAGGPSRGEPGYPIVARARYGPTTFLLEVLAPEV
                     ARACEPGQFVMVRIDETGERIPLTISDFDRDRGTLTMVVQAVGKTTFEMLDLAEGDRL
                     LDVVGPLGRASKVERRSKVVLVGGGLGVAPIFPLLRRHVDLGSGTISIVGFRSADRAF
                     WTERFQALSDELFVATDDGSLGAQGTVADVLADVVGDRDDVEEVFAIGPLPMMRACAE
                     LTRPRGIRTVVSLNSIMVDGTGMCGSCRVTVGGQMRFACVDGPDFDGHEVDFDELALR
                     LGRFRYEERECLHRYEEERARLQELGIGGPDPARLPARTRAFPMAGLPVPEPVPRALR
                     PTGPKKVREVSPTRQEMPVLDPVSRVQTFDEVALGYDLEAALVEADRCIRCTKPKCIE
                     GCPVRIDIPGFIAELAAKDLEASYRILKASNTLPAVCGRVCPQEIQCEARCVVGKKRD
                     PVAVGRLERFVADFAAGRGWDESPSRPASGKRVAIVGSGPAGLACAGDLARAGVDVTV
                     YEALHVAGGVMKYGIPEFRLPNDTIDIEIDELSELGVRFELDAIVGQLFTLPKLLGEM
                     GYDAVFLGTGAGTPRFLGIPGESLNGVVSANEFLTRVNLMQGFRQPLYDTPVGMGKRV
                     AVVGAGNTAMDASRVALRMGADRVILVYRRSERESPARAEELHHAIEEGVECRWLTNP
                     TRIRGDDDGWVTGIEVVRMELGEPDGSGRPSPRPLPGTEQVLDVDTVIEALGTAPNPT
                     MARTAQGLDTDDRGYLRVDPETQMTSMPGVFAGGDIVTGSATVILAMGAGRRAAAGIL
                     AHLGLDGRKDEHPVGIGATTSRR"
     gene            3780..>4093
                     /locus_tag="VF036_03340"
     CDS             3780..>4093
                     /locus_tag="VF036_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6264296.1"
                     /translation="MDVNMVYRDEVFSITETDTLEEAARRMHGNEVGSLVVLDGDRCV
                     GIITERDLTRAIAEGENTHRARVSGYMSIRPITVTPDTGIREAAAEMLETEIRHLPVG
                     IGG"
BASE COUNT          633 a         1352 c         1480 g          628 t
ORIGIN      
        1 ccccggacgt tcctcccgag gagccggagc ttactaggca tcactcgggc gtgtgcccgc
       61 accccatcga ggatggtgta tcaagggtgg gaggtgccga agcctggtac gggtttcccg
      121 gcttcgtcgc ccagcccacc cggcgtgcga ggagtaggga gatggaactc ggtgcgttct
      181 cggtcagttt ggcggtcagg gacctcggcg cgtcgcggac gttctacgag tcgctcggct
      241 tccaggtcgt catgggcgac gcgtccgaga actggttgat cctccggaac ggcgacacca
      301 cgatcggcct cttccatggc atgttcgagc ggaacgtcct gacgttcaac cccggctggg
      361 atcgcgacgc acggccgctc acggtcttca cggacgtccg tgagctccag cgcgagatcc
      421 gggagcgagg cgtggcgatc gagcagccgg cggatgagtc atcgaccgga cccgcaagct
      481 tcatcgtcgt ggacccggaa gggaacccga tcctcttcga tcagcacgtc tgacgccgct
      541 gcgacctcct gtggggctcc gcggtccgat catctgggcc cggtccgcac gccgcaggac
      601 ggagagttcc gggcatgagc cgcgcccgga tgtgcatggc cagccgcgtc agcgcaccac
      661 gaggaccggg cactcggcat gatggatcac cttgtgggcg atgctgccga gcagaaggcc
      721 ggcgaagtcg gagagcccac gggtccccat cacgatgacg tcggcatcga acgccttggc
      781 cgcatcgagg agcgcgggag cggtcgatcc cgcccgggcc gtcaccgccg aaccggtggc
      841 cttcagcccg gcgtcgttca actcgcgaac gacgccatcc acgaggctct gcgcgtcgct
      901 cggcgtttcg atcgacgcca ccacgcccac ttcgtgcacg tgaacgacca gcacctcggc
      961 accgtctcga ccgagtcctg cggtgacctc ggctgccttc ctcgagtgct cggagccgtc
     1021 gaccgccaag acgatcttct cgaacatcac ggcacctccc ttccgcgggg gagcctactc
     1081 cagcctgctg ccgggagcga ctcgaaccga accccgggcg tcgtcacggt agggccgcgt
     1141 tgagcggccg tcgccggccg gtatccgctt cctcggaggt ggtgatcgtg tgcgccgtcg
     1201 agaagcccgc gccgcggtct ggccggtgcc ggtcggtcca cccgggacgg gcccttcggc
     1261 cctatccgac gctcgcccat ccccacaccc tgggtgcgga ggcacggtat ggatggacgg
     1321 aacggtcggt tcggacgatc ggcgggcggc ccctccaggg gcgagccggg ctatccgatc
     1381 gtcgctcggg cgcggtacgg gccgacgacc ttcctgctcg aggtcctcgc gccggaggtg
     1441 gcgagggcat gcgagccggg tcagttcgtc atggtccgga tcgacgagac cggtgagcgg
     1501 atccccctga cgatctccga cttcgatcgg gatcgcggga cgctcaccat ggtggttcag
     1561 gcggtcggga agaccacgtt cgagatgctg gacctggcgg agggggaccg actgctggac
     1621 gtcgtcggac cactcggccg cgcgtcgaag gtcgagcggc ggtccaaggt ggtcctggtg
     1681 ggcggcggcc tcggggtggc gccgatcttc cccctccttc gccgccacgt cgatctcggc
     1741 tcggggacga tctcgatcgt gggattccga tcggccgatc gcgcgttctg gacggagcgc
     1801 ttccaggccc tgtccgacga gctgttcgtc gccacggacg acggttccct cggtgcgcag
     1861 ggcacggtcg ccgacgtcct cgcggacgtc gtgggcgacc gcgacgacgt tgaggaagtc
     1921 ttcgcgatcg gtcccctccc gatgatgcga gcatgcgccg agctcacgcg gcctcgcggg
     1981 atccgcacgg tcgtgagcct gaactcgatc atggtcgacg gcaccgggat gtgcggcagc
     2041 tgccgggtga cggtgggcgg ccagatgcga ttcgcgtgcg tcgatggccc cgacttcgac
     2101 ggacacgagg tcgacttcga cgagctcgcc cttcgcttgg gccggttccg gtacgaggag
     2161 cgcgagtgcc tccaccgcta cgaggaggag cgggcgcggt tgcaggagct cgggatcggt
     2221 ggccccgatc cggcccggct cccggcacgg acgcgcgcat tcccgatggc ggggctcccc
     2281 gtaccggaac cggtacccag ggctctccgc ccaaccggtc cgaagaaggt gcgcgaggtc
     2341 tcgccgacac gccaggagat gcccgtcctc gatcccgtgt cgcgcgtgca gacgttcgac
     2401 gaggtggccc tggggtacga cctcgaggcc gcgctcgtcg aggccgatcg atgcatccgc
     2461 tgtacgaagc cgaagtgcat cgagggatgt ccggtgcgga tcgacatccc cgggttcatc
     2521 gccgagctcg cggccaagga cctcgaggcc tcctaccgga tcctgaaggc gtcgaacacc
     2581 ctaccggcgg tctgtgggcg ggtctgtccg caggagatcc agtgcgaggc acgatgcgtg
     2641 gtcggcaaga agcgcgaccc agtggcggtc ggtcgcctcg agcggttcgt ggccgacttc
     2701 gccgccggcc gcggctggga cgagtcgccg tcgcggcccg ccagcggcaa gcgcgtcgcg
     2761 atcgtgggat cggggccggc gggcctcgcg tgcgcgggtg acctcgctcg ggccggcgtc
     2821 gacgtgacgg tctacgaggc gctccacgtc gcaggcggcg tgatgaagta cgggatcccc
     2881 gagttccggc tcccgaacga cacgatcgac atcgagatcg acgagctgtc tgaactcggc
     2941 gtccggttcg agctcgacgc gatcgtgggg cagctgttca cgctccccaa gctgcttggc
     3001 gagatgggat acgacgccgt cttcctcggg accggcgccg ggaccccccg tttcctcggg
     3061 atcccgggag agtcgctgaa cggcgtcgtg tcggcgaacg agttcctgac acgcgtgaac
     3121 ctgatgcagg gcttccggca gccgctgtat gacacgccgg tcggcatggg caagcgcgtc
     3181 gcggtggtag gcgccggcaa caccgcgatg gacgcgagcc gggtcgcgct ccggatggga
     3241 gcggatcggg tgatcctcgt ctaccgacgc tcggagagag aatcgccggc gcgggccgag
     3301 gagctccacc acgcgatcga ggagggtgtg gagtgtcgct ggctgacgaa ccccacacgg
     3361 atccgcggtg acgacgacgg ctgggtcacc gggatcgagg tggtccggat ggagctgggc
     3421 gagcccgacg gatccggccg gccgagtccg cgaccgctgc ccggtaccga acaggtcctg
     3481 gacgtcgaca cggtgatcga ggcgctcggc accgcgccga acccgacgat ggcgcgcacg
     3541 gcgcagggtc tcgacacgga cgatcgaggc tacctgcggg tcgacccaga gacgcagatg
     3601 acctcgatgc cgggggtctt cgcgggggga gatatcgtga ccggctcggc cacggtgatc
     3661 ctcgcgatgg gcgcggggcg gcgcgcggca gccgggatcc tcgctcacct cggcctcgac
     3721 gggaggaagg acgaacatcc ggttgggatc ggagcaacga cgagcaggag gtgaccacga
     3781 tggacgtcaa catggtctat cgagatgagg tgttctcgat caccgagacg gacacgctcg
     3841 aggaggcggc gcgacggatg cacggcaacg aggtcggatc gctcgtggtg ctggacggcg
     3901 accgatgcgt tgggatcatc accgagcgag acctcaccag ggcgatcgcc gagggcgaga
     3961 acactcaccg cgcgagggtc tccggctaca tgtcgatccg accgatcacg gtgaccccgg
     4021 acacggggat ccgtgaggcg gccgctgaga tgctcgaaac ggagatacgc cacctaccgg
     4081 tcggcatcgg cgg
//
LOCUS       DASUVE010000157         2827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_78359, whole genome shotgun sequence.
ACCESSION   DASUVE010000157 DASUVE010000000
VERSION     DASUVE010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78359"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VF036_03345"
     CDS             <1..691
                     /locus_tag="VF036_03345"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX6264297.1"
                     /translation="EELLGLLRDRMDTGRIVGTGDPQGWNLRASETAPESSARPLVKR
                     RKTEQAHICLGTNGLPRSDPDRFPFLIVNGALGTGMSSRLFQEIREKRGLVYSVYSYH
                     SQYTEAGLFSCYAGTTPARAAEVIGLLRRELEDVADGGLTAEEFDRAKGHVKGSMVLS
                     LEDPGSRMSRIGKSEISHGEILTVNQSLKRVEDVTLEDARRVAKRVLAQPMTLTVLGP
                     FAQRDLAGVMS"
     gene            688..1458
                     /gene="dapB"
                     /locus_tag="VF036_03350"
     CDS             688..1458
                     /gene="dapB"
                     /locus_tag="VF036_03350"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007533340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX6264298.1"
                     /translation="MIRVGVLGATGKVGSEVCRAVHADADMALVAGISRSKAGEKAAD
                     ALGLEGSDVVLAENLDAFVDSSVEVAVDFTGGGFAAEHVAWAIEHGVHIVVGSTGFEV
                     DDGWREAPIGVFIAPNFAIGAVLMQRFAREAARHLAAVEIVELHHDGKADAPSGTAAA
                     TADAVADARPEAWSGPSSESVPGVRGGERAGIRIHSIRLPGLVAHQEVIFGGQGQTLT
                     IRHDSTDRTSFVPGVLMAIRWVPSHPGLNLGLDAILDN"
     gene            1499..2713
                     /locus_tag="VF036_03355"
     CDS             1499..2713
                     /locus_tag="VF036_03355"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6264299.1"
                     /translation="MDIEIRPITYEERPEWVHAAETAFSSVAKEDEVEAWLPVIEPDR
                     SFAAIDGGRIVGTSGAITFRMMVPGGAMIPTAGITAVGVHPTHRRRGINTGMMRLLLD
                     QAAERGEPLAALFASEGAIYGRFGYGLAGMFGEFEAESARMAFVRGYQPSGHVELVSK
                     DDALPVIDEVYEASMRPGGVERNQVLRDHSFATVGEDKDRPWMYAIHRDEAGQADAYA
                     VYWMKHEWPRSVPSGKITVGECVGATPSGNADIWRYLFDIDLVATVEAWNRPADEPLL
                     QLVREPRRLRFSINDGLWIRLLDVVAALEARRYRTDGRIVFEIADLFRPENDGRYELA
                     VEGGTGRCARIDDDADLAGTINMLGATYLGGASFRQLWWASQVDERTAGSVERADAMF
                     ASTPAPWCVVDF"
BASE COUNT          438 a          934 c         1021 g          434 t
ORIGIN      
        1 cgaggagctg ctcggcctgc tccgcgatcg gatggacacc ggacggatcg tcgggaccgg
       61 tgacccgcag ggatggaacc tccgggcgag cgagaccgcg cccgagtcct ccgccaggcc
      121 gctggtgaag cggcggaaga ccgagcaggc gcacatctgc ctcggcacga acggactccc
      181 gcggagcgat cccgaccggt tcccgttcct gatcgttaac ggggcgctgg gcaccgggat
      241 gtcctcccgg ctgttccagg agatccggga gaaacgcggc ctcgtctact cggtctacag
      301 ctaccactcg cagtacacgg aagccggtct gttctcctgc tacgcgggca ccacgccggc
      361 ccgggccgcc gaggtgatcg gcctccttcg gcgggagctc gaggacgtcg cggacggcgg
      421 tctcacggcc gaggagttcg accgagcgaa ggggcacgtg aagggctcga tggtcctctc
      481 gctggaagac cccgggagca ggatgtccag gatcgggaag tccgagatct cccacggcga
      541 gatcctcacg gtgaaccagt ccctgaaacg ggtggaggac gtgacgctgg aggacgcgcg
      601 gcgcgtggcc aagcgggtgc tggcgcagcc gatgacgctg accgtcctcg ggccattcgc
      661 gcagcgcgac cttgcggggg tcatgtcgtg atccgcgtcg gcgtgctcgg cgccacgggg
      721 aaggtgggat ccgaggtgtg tcgcgccgtg cacgccgacg cggacatggc gctggttgcc
      781 gggatctccc gctcgaaggc gggcgagaag gcagccgacg cgctcgggtt ggagggctcc
      841 gacgtcgtct tggccgagaa cctcgacgcg ttcgtggact cgagcgtcga ggtggcggtg
      901 gacttcaccg gcggaggctt cgccgcggag cacgtggcgt gggccatcga gcacggcgtg
      961 cacatcgtgg tcggctccac cggcttcgaa gtcgacgacg gctggcgcga ggcaccgatc
     1021 ggcgtgttca tcgcgccgaa cttcgcgatc ggcgcggtgc tgatgcaacg gttcgcgcgg
     1081 gaggcagccc gtcacctggc cgccgtcgag atcgtcgagc tccatcacga cggcaaggcg
     1141 gacgcgccgt cgggcaccgc tgccgcgacg gccgacgccg tcgccgacgc gcgcccggag
     1201 gcatggtctg gcccgtcgtc ggaatcggtg ccaggtgttc gcggcggcga gcgggcgggc
     1261 atccggatcc actcgatccg gctgcccggt ctcgtcgcgc accaggaagt gatcttcggc
     1321 ggtcaggggc agaccctcac gatccggcac gattcgacgg atcggacctc gttcgtcccc
     1381 ggcgtgctga tggcgatccg atgggtgccc agccacccgg ggttgaactt ggggctcgac
     1441 gcgatcctcg acaactgatc gcgggacgaa ctgctcgcgc ccgctaggct cgggcgtcat
     1501 ggacatcgag atccgtccaa tcacctacga ggaacggccc gagtgggtcc atgccgccga
     1561 gaccgccttc agctccgtgg cgaaggagga cgaggtcgag gcgtggctcc ccgtcatcga
     1621 gcccgatcgc tcgttcgccg cgatcgacgg cggccggatc gtcggcacgt cgggtgccat
     1681 caccttccgc atgatggtcc ccggtggcgc gatgatcccg acggccggga tcacggcggt
     1741 gggcgtccat cccacccatc gtcgccgggg gatcaacacc gggatgatgc ggctcctcct
     1801 cgatcaagca gcggaacgag gcgagccgct cgccgccctt ttcgcctcgg agggcgcgat
     1861 ctacggacgc ttcggctacg gcctcgccgg gatgttcggc gagttcgagg cggagtccgc
     1921 ccggatggca ttcgtgcgcg ggtaccagcc gagcggccac gtcgagctgg tatcgaagga
     1981 cgacgcgttg cccgtgatcg acgaggtgta cgaagcctcg atgcgccccg gtggggtgga
     2041 acgcaatcaa gtcctccgcg atcactcgtt cgcgacggtg ggggaggaca aggatcggcc
     2101 gtggatgtac gccatccacc gcgacgaggc agggcaggca gatgcgtacg ccgtgtactg
     2161 gatgaagcac gaatggcccc gttccgttcc gtccgggaag atcaccgtag gggaatgcgt
     2221 gggggccact ccgtccggca acgccgacat ctggcggtac ctgttcgaca tcgacctcgt
     2281 ggccacggtg gaggcatgga accgtcccgc cgacgagccg ctcctccagc tcgtccgtga
     2341 gccacgacgc ctgcggttct cgatcaacga cggcctgtgg atccggctcc tggacgtcgt
     2401 ggcggccctc gaggctcgcc gctaccggac cgacggccgg atcgtcttcg agatcgcgga
     2461 tctgttccgg cccgagaacg acggccggta cgagctcgcc gtcgaaggcg ggaccggtcg
     2521 ctgcgctcgt atcgatgacg acgccgacct ggcggggacg atcaacatgc tgggagccac
     2581 ctacctgggc ggggccagct tccgccagct ctggtgggcg agccaggtcg acgagcggac
     2641 ggccggctct gtcgagcggg ccgacgcgat gttcgcgtcc acgccggctc cctggtgcgt
     2701 cgtcgacttc tgatcccgac tctctcgcca gagactctct cgcgtttgag cgcaacggcg
     2761 ggatagggtc cgcccaccta ccccggagga ggagcgatga ctcgaggatc aggtggccac
     2821 ggcacga
//
LOCUS       DASUVE010000158         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1021594, whole genome shotgun sequence.
ACCESSION   DASUVE010000158 DASUVE010000000
VERSION     DASUVE010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021594"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VF036_03360"
     CDS             complement(<1..969)
                     /locus_tag="VF036_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX6264300.1"
                     /translation="MKAIVQDRYGSADVLEFRDIEEPLVGENDVLVRVDAAGCGPDVW
                     HLMTGLPYFVRLMPGFYKMRAGVRGRDLAGTVEAVGASVTGFRPGDEVMGIGEGSFAE
                     LVIARPDRLVPKPAGLSFEDAAAAPISGLTALQAIRDVGKVQPGQRVLVIGAGGGVGT
                     LTVQVAKAFGAQVTGVGSTSKLDLIRQVGADEVIDYTREDFTEGTRRWDVIVDTAGRR
                     PLRVLRRALAPKGTLAIVGGEGGGRWTGGFLGTMLRAPLLSLFSGQKFRPVVSKENLE
                     DLQSLAELIADGKVTPVVGKTYPLIEAPDAIRYLQEGHAAGKVVVTV"
     gene            1109..1798
                     /locus_tag="VF036_03365"
     CDS             1109..1798
                     /locus_tag="VF036_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEX6264301.1"
                     /translation="MATKIDQEAKPRSPLNKERILRAAVALADADGVDSLSMRKIAEE
                     LDVVPMALYRHVANKDEMLDGMVDLVVAEIDPPETGLDWKGAMRRRILSARRMLQRHP
                     WAARVLESRTDPTPAVIGYIDEMIGLFLAGGFTIELTHHALHAMGSRMFGFTQELFDD
                     TPDLDPEAEAAILEAMSQAYPNIGRMIAIAVGSHDDASRVGDRGCDDQFEFEFALDLM
                     LDGLERLRDVG"
     gene            complement(1820..>2502)
                     /locus_tag="VF036_03370"
     CDS             complement(1820..>2502)
                     /locus_tag="VF036_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6264302.1"
                     /translation="RLRTIGTDNLVAAARTAGARRFVTQGFAGWPYASAGSALRTEED
                     PFESSPPEEMRSTVDALRHLETAVLGVEDLEGIVLRYGGFYGPGTSLGDGGEMVEGIR
                     KRQLPIVGDGAGVTSFLHVADAAAATVAALERGMSGVYNVVDNEPAPSSLWIPELAKA
                     VGAKPPRHVPVWLAKPLIGEAGVWLMTKAPGASNAKAKVELGWTPRYASWRIGFREGL
                     GRETAMCA"
BASE COUNT          402 a          919 c          808 g          373 t
ORIGIN      
        1 gacggtgacg accaccttgc cggcggcgtg cccttcctgg aggtaccgga tcgcatccgg
       61 tgcctcgatc agcggatagg tcttcccgac gacgggcgtg accttcccgt ccgcgatgag
      121 ctcggccagc gactggagat cctcgaggtt ctccttcgag acgacggggc ggaacttctg
      181 accggagaac agagacagca atggcgcccg gagcatcgtg cccaggaagc ctccggtcca
      241 gcggccgccg ccttctcccc cgacgatcgc gagggtgccc ttcggcgcga gggctcgccg
      301 aaggacgcgg agcggacgcc ggcccgccgt atcgacgatg acgtcccagc gcctcgtccc
      361 ctcggtgaag tcctcgcgcg tgtagtcgat cacctcgtcg gcacccacct gccggatcag
      421 atccagcttc gaggtgctgc cgacgccggt aacctgggcc ccgaacgcct tcgcgacctg
      481 cacggtgagc gtgccgacgc ctccaccggc gccgatgacc aacacccgct gccccggctg
      541 caccttcccg acgtcgcgga tcgcctgcag ggcggtgagg ccggagatcg gggcggccgc
      601 ggcatcctcg aaggagagcc cggcgggctt cgggaccaac ctgtccggtc gagcgatcac
      661 gagctccgcg aaggagccct cgccgatacc catcacttcg tcgccgggcc ggaatccggt
      721 gaccgacgcg ccgaccgcct cgacggtccc cgcgaggtcc cggccgcgga caccggccct
      781 catcttgtag aagccgggca tcaatcgcac gaagtacggc aagcccgtca tcaggtgcca
      841 cacgtccggg ccgcagccgg ccgcgtccac gcgaaccagc acgtcgttct cgccgaccaa
      901 cggctcctcg atgtctcgga actccaatac gtccgctgac ccgtaccgat cttgcacgat
      961 cgctttcatc cagatcctct cccctgctgt ccatgctggc gtaccttctg tgtacgccgt
     1021 acacttcgat gggtggagag tacgtgtacg gcgtacactt gtcaagccgg acgccccgga
     1081 cggggctcca cgagagaaga tgggcgcgat ggccacgaag atcgaccagg aagcgaagcc
     1141 ccggtccccc ctgaacaagg agcggatcct ccgggccgcc gtggcgctcg ccgacgcgga
     1201 cggggtcgac tcgctgagca tgcgcaagat cgccgaggaa ctcgacgtcg tcccgatggc
     1261 cctctaccgc cacgtggcca acaaggacga gatgctcgac gggatggtcg atctcgtcgt
     1321 cgccgagatc gacccgcccg agacgggcct cgactggaag ggggcgatgc gccggcggat
     1381 cctctccgcc cgccggatgc tccagcggca tccctgggcg gctcgcgtgc tcgagtcgcg
     1441 gaccgacccc acgcccgccg tgatcggcta catcgacgag atgatcgggc tgttcctggc
     1501 cggcggcttc acgatcgagc tcacccacca cgccctgcac gcgatgggca gccggatgtt
     1561 cgggttcacg caggagcttt tcgacgacac gcccgacctc gaccccgagg ccgaggcggc
     1621 gatcctggag gcgatgagcc aggcctatcc gaacatcggc cggatgatcg cgatcgcggt
     1681 cggctcgcac gacgacgcgt cgcgggtcgg cgaccggggc tgcgacgacc agttcgagtt
     1741 cgaattcgcg ctcgacctga tgctcgatgg cctcgagcgc ctcagggacg ttggctgacc
     1801 ccggacaccg acctgaccgt caggcgcaca tcgccgtctc ccgtccgaga ccttcgcgga
     1861 acccgatgcg ccacgaggcg taccggggcg tccaccccag ctccaccttc gccttcgcgt
     1921 tcgaagcacc gggcgccttc gtcatcaacc acacgccggc ctcgccgatc agcggcttcg
     1981 ccaaccagac gggcacgtgg cggggcggct tcgcaccgac cgccttggcc agctcgggga
     2041 tccacagaga cgacggggcc ggctcgttgt ccacgacgtt gtagacgccg gacatccctc
     2101 gctcgagggc tgcgacggtc gctgccgccg cgtccgcgac gtggaggaac gatgtcacac
     2161 cggccccatc gccaacgatc gggagctgcc gcttccggat cccttcgacc atctccccgc
     2221 cgtcgccgag cgacgttccc ggtccgtaga agccgccgta gcggagcacg atgccttcga
     2281 gatcctcgac acccagcacc gcggtctcca ggtgtcggag cgcatccacg gtcgaacgca
     2341 tctcttccgg cggggaggac tcgaacggat cctcctcggt ccggagggcg gagcccgcgc
     2401 tcgcgtaggg ccatcccgcg aagccctgcg tgacgaaccg tcgggcgccg gccgttcgtg
     2461 cagccgcgac gaggttgtcc gttccgatcg tccggaggcg gt
//
LOCUS       DASUVE010000159         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1399576, whole genome shotgun sequence.
ACCESSION   DASUVE010000159 DASUVE010000000
VERSION     DASUVE010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399576"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /gene="speB"
                     /locus_tag="VF036_03375"
     CDS             <1..1029
                     /gene="speB"
                     /locus_tag="VF036_03375"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX6264303.1"
                     /translation="DRSFRVPESTLYASEDAEFDEFGTNELPQYSDFRGSFSKVPWAL
                     DEEAIRDAGADIAIVGAPYDEGTSARPGARFGPMAIRTAHVTAGSPWAWSLQTEVSPF
                     DVLTVVDAGDAPIVPGRPERSLRVIHEKVRRVARAGAIPLVLGGDHSITYPSAAAVAR
                     VHDPARVGIVHFDAHADTAIDQWGSLIGHGQPMRRLIEEGWIAGPNFVQVGLRGYWPD
                     RETFEWMRGQGLRWHTQVEMEERGVEAVIDEAIAQALDGPDRVYLSVDIDVVDPGMAP
                     GTGTPESGGMLGRELLRAVRRIVGAVDLAGMDVVEVSPPYDHAEITAMLAVNVVMEAI
                     SALAARRG"
     gene            1058..1279
                     /locus_tag="VF036_03380"
     CDS             1058..1279
                     /locus_tag="VF036_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018849837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX6264304.1"
                     /translation="MCRSIKRLREPHGQGHATDEEIREAARQFVRKVSGFGKPTARHA
                     EAFEAAVDEVTDSSRRLLETIDATLRSTA"
     gene            1276..>2116
                     /locus_tag="VF036_03385"
     CDS             1276..>2116
                     /locus_tag="VF036_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6264305.1"
                     /translation="MTGSPLDPSPEQMRAMGRAAVDYVIDFIHGLSDAPARNVDGALE
                     AALKLKGSPPEEGGSFDEVFAQFRDAAARAYETCGPGYLPYVPGGGLYASALGQFLAM
                     SVNRFPNLWEVAPGLVQIEQNVIRWLCDLFGYPDEARGILTTGGSIANLSAVVTARHV
                     KLGEDFGDGTYYVSEQAHASVPKAAAIAGMPRRAMRLVPVDAELRMDAEALKSMVAED
                     RVAGHRPFLVVPAAGTTNTGAIDPMDAVADVAEAEDLWMHVDAAYGGFFHLTDRGRER
                     FRGI"
BASE COUNT          314 a          709 c          781 g          312 t
ORIGIN      
        1 gatcgttcgt tccgggtccc ggagagcacc ctctacgcga gcgaggacgc cgagttcgac
       61 gagttcggca cgaacgaact gccgcagtac tcggacttcc gcggatcctt ctccaaggtc
      121 ccgtgggctc tcgacgagga agcgatcagg gatgccggcg cagacatcgc gatcgtcggc
      181 gctccgtacg acgaggggac ctccgcgcgg ccgggggcgc ggttcggacc gatggcgatc
      241 cgaaccgccc acgtgacggc gggaagcccg tgggcgtggt cgctccagac ggaggtatcc
      301 cccttcgacg tcctcacggt ggtcgatgcg ggcgacgcgc cgatcgtccc ggggcgaccc
      361 gagcgcagcc tgcgcgtgat ccacgagaag gttcgtcgcg tcgcccgcgc cggtgcgatc
      421 ccgctcgtcc tgggcggcga ccactcgatc acgtatccgt ccgccgccgc ggtcgcccgg
      481 gtgcacgacc cggcgcgcgt cgggatcgtg catttcgacg cgcacgccga cacggcgatc
      541 gaccaatggg gatcgctgat cggccacggg cagccgatgc gtcgactgat cgaggagggg
      601 tggatcgccg ggccgaactt cgtccaggtc gggctgcgcg gctactggcc tgatcgggag
      661 acgttcgagt ggatgcgggg tcaggggctg cgctggcaca cgcaggtcga gatggaggag
      721 cggggcgtcg aggccgtgat cgacgaggcg atcgcgcagg cgctggacgg ccccgaccgg
      781 gtctatctct cggtcgacat cgacgtcgtc gacccgggga tggctcccgg caccggaacg
      841 cccgaatccg gcgggatgct cgggcgggag ctgctccgcg cggtccgacg gatcgtgggt
      901 gcagtggacc tcgccgggat ggacgtcgtg gaggtctccc ctccgtacga tcacgcggag
      961 atcacggcga tgctcgcggt caacgtggtc atggaggcga tcagcgccct cgcggcccga
     1021 cggggctgaa cgcgcgtcgc gcggtaccct cccgctcatg tgccgaagca tcaagcgact
     1081 gcgggaacca cacggacagg ggcatgccac ggatgaggag atccgggagg cggcgcgtca
     1141 gttcgttcgc aaggtgagcg ggttcgggaa gcccacggcg cgacacgccg aggcgttcga
     1201 ggcggccgtc gacgaggtca cggattcgtc ccggcgactc ctcgagacga tcgacgccac
     1261 cctgcggagc accgcgtgac cggctccccc ctcgatccgt cgcccgagca gatgcgggcg
     1321 atggggcggg cggcggtcga ctacgtgatc gacttcatcc acggcttgtc cgacgccccg
     1381 gcccggaacg tcgacggtgc gctcgaggcc gccctgaagc tgaagggctc gccgcctgag
     1441 gaaggcggct cgttcgacga ggtcttcgcc cagttccgcg acgcggctgc gcgggcctac
     1501 gagacctgtg gcccgggcta tctgccctac gtccccggtg gcggtctgta cgcgtcggcg
     1561 ctcgggcagt tcctcgcgat gtccgtgaac cgcttcccga acctctggga ggtcgcgccg
     1621 gggctggtcc agatcgagca gaacgtgatc cggtggctct gcgacctctt cggctacccg
     1681 gacgaggcgc gcggcatcct cacgaccggc ggctcgatcg cgaacctctc ggcggtcgtc
     1741 accgcacgtc acgtgaagct cggggaagac ttcggcgacg gcacgtacta cgtgagcgag
     1801 caggcgcacg cgtccgtgcc gaaggccgcc gcgatcgccg ggatgccgcg tcgcgcgatg
     1861 cgactcgtgc ccgtcgatgc ggagcttcgg atggacgccg aggcgctcaa gtcgatggtg
     1921 gccgaggacc gcgtcgccgg acaccgaccg ttcctcgtcg tgccggccgc cggtaccacg
     1981 aacaccggag cgatcgaccc gatggacgcg gtggccgacg tggccgaggc ggaggatctg
     2041 tggatgcacg tcgatgccgc gtacggcggc ttcttccatc tcacggaccg gggacgagag
     2101 cggttccgcg ggatcg
//
LOCUS       DASUVE010000160         6011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_646127, whole genome shotgun sequence.
ACCESSION   DASUVE010000160 DASUVE010000000
VERSION     DASUVE010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646127"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VF036_03390"
     CDS             <1..408
                     /locus_tag="VF036_03390"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX6264306.1"
                     /translation="IRLIRHQLEPLAVRLVVEKITEEELQEAERLRERMAKERDLGNW
                     VELNTQFHLVFHDSTGVSRLASILKGFEEASTVYVAQAQRWHPEIRRRADDEHQALIE
                     AFRSRDADRAAAVMQGHAAMPIEMTRPEERGTG"
     gene            454..1446
                     /locus_tag="VF036_03395"
     CDS             454..1446
                     /locus_tag="VF036_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6264307.1"
                     /translation="MDTARLGIVGLGWFGGVLAKSARASGVADVVSCYARTEETRAAF
                     AEEHGCRAAGDLGELLDDPEVDGVIVATPHSTHADIAVAAAEAGKHMFVEKPLTLTVA
                     DAKRVGDAASRAGVTVQVGHNRRRQPANRKIKAMIDAGELGTVLQLEGMHSAAGGHKP
                     DLPAWRKDPAECPFGGMTALGVHTVDTFHYFMGPGKRVTALSTRFRGFNDLDDATTVL
                     IEYESGPIASINTTYFAPPVVSLSVFGSDAAAWNEEDGTRLYTQVRSDPARTEHEVET
                     IDTMHDEIVEFARCIGGEAEPETGMPEAIEVAAVLEAIGRSVASGSVVELADLR"
     gene            complement(1443..2342)
                     /locus_tag="VF036_03400"
     CDS             complement(1443..2342)
                     /locus_tag="VF036_03400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6264308.1"
                     /translation="MSDTSGRRDHLRGLALCAIGVVVISPDALIVRSVEANAWTTVAW
                     RGIFTVVGTVALLRLLRPADGHRSWRPTGPHVFAGALFAAASIAFVSALDRTDAASVL
                     VIIAAGPLIASVLGRVFLRQRAAARTWLTGIAVVAGLSLILGGSIGQGDLDGDLLALA
                     GATCFAGYLTVSRAARPADMTPAIAIGGVGAAIAGGIAGADVSIDASDMLLLALLGVV
                     ILPISLTLITRATRHVPAPEVNLVALLETLLGPLWVWLVIGERPSAEVLAGGVLVVGA
                     VTIHSALALRAERDEDTPAGVIV"
     gene            2447..3517
                     /locus_tag="VF036_03405"
     CDS             2447..3517
                     /locus_tag="VF036_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264309.1"
                     /translation="MAGRRVLRGIAVLAAATLFAVSCGDDGGGGGGGGGGGEESNIIR
                     FTFAPDPAWDWMKDQGILEAMEQESGFRIIQLATWDEWATFAGGHADVVSTGTYETPL
                     LEEQGIETVTFGQFNMNKDVLVTAHPEYQTASDFPAGCKIATESVTGNTIIWGSLINA
                     IDGREVAEDSDDIGIVTADYQVIPNLLREDEACAGILDPTQVIPDMASGDLTVMYDGK
                     SASQLYGEEIVPGHEGVLSNAFVSRAEWFDANPEAAAFFQEVWDCAMVQWAENRDEII
                     DTYPQHFAVETEAEAQFMKDYFENTFDWFVESPYLTEEWIEGERPVFDLVQDAEVIPA
                     DAEFPRHEVMEDPEGEPCPEGF"
     gene            3573..4427
                     /locus_tag="VF036_03410"
     CDS             3573..4427
                     /locus_tag="VF036_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6264310.1"
                     /translation="MAATVETEKTLEELEQEAIDALASSGRRTRAKVLYSLLGAASFI
                     AVWAYWSTVLEPFILPSPLAVAQRMWELLVSGVVFTNFLSSFLKTMAGWAMALIIGIP
                     VGMLMGRYRYARAFFHDFVYLFANVPLLVYAVIAIVVFGISPWGPAFVVALEAFTGIA
                     INVAAGVESVDRGLLAMSRSFRRSSRRTLRSIVVPSVVPFLFASGRVSFANSWKLAAL
                     AETFGGYLGVGYQLEKSFQIYSVVDLLAWMFFFVLFVILVERLLIAPAERRVFRWRDP
                     AGAREQVS"
     gene            4424..5272
                     /locus_tag="VF036_03415"
     CDS             4424..5272
                     /locus_tag="VF036_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6264311.1"
                     /translation="MSDVATVDGLGSAEIPEFLLAPPPRSRWWWLTSDRAARYTARVA
                     FLLVWQWAGSTFEDIPTPLGTVEALIEEFQRGEVFPNLAISLVRAVIGLSIVLVLGVS
                     IGIAMARWWRVRYFFTDLILVGITLPAFIWALLVVMWWGFSNIGPITVCVLSATPQLV
                     VNTFQGATATPGRLLAMSDAYRVGTRRQFRWLMIPSMMEYIAAGFRSAVLAGWGAVLL
                     VEWFGNDRGVGFRAHYWYDARSFEGMMAWGLVMMITILAFDRVVMDRVVRRFRGWRTG
                     GQQWSR"
     gene            5316..>6011
                     /locus_tag="VF036_03420"
     CDS             5316..>6011
                     /locus_tag="VF036_03420"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6264312.1"
                     /translation="MARLVARDVKKVFPPTRAGQDPVFALRNVSFEAEGHTFVTLVGP
                     SGCGKSTFLNMVSGIETPSAGTLEVIGRSEGDARIGYVFQDPRLLPWKTVLDNMVYVL
                     DEGSSEERTERGRKYLDLVNLGAKAEMFPGQLSGGMQQRVGIARALSIEPDLLLMDEP
                     FSHLDAITARDLRVELQRLWLDTGSTILFVTHDVLEAVLLSSRVLMMSSQGVIFRDLT
                     IDLPFPRRQTDREV"
BASE COUNT          969 a         1945 c         2128 g          969 t
ORIGIN      
        1 atccgtctga tccgccatca actcgagccg ctggcggttc gcctcgtggt cgagaagatc
       61 acggaggagg agctccagga agccgagcgc ttgcgggagc ggatggcgaa ggaacgcgat
      121 ctcggcaact gggtcgagct gaacacacag ttccacctgg tcttccatga ttcgacgggg
      181 gtgtcgcgcc tcgcctcgat cctgaagggg ttcgaggagg cgtccacggt ctacgtcgca
      241 caggcgcagc ggtggcatcc cgagatccgg cggcgtgcgg acgacgagca ccaggctctg
      301 atcgaggcgt tccggagtcg tgacgccgat cgcgccgcgg ccgtgatgca ggggcacgcc
      361 gcgatgccga tcgagatgac ccggcccgag gaacgcggaa cgggctgagc gggacagcac
      421 atccgggttc gaacatcgaa gggagcgacg gacatggaca cggctcggct cggcatcgtc
      481 ggactcggat ggttcggagg tgtccttgcg aagagcgctc gggcgtccgg cgtcgcggac
      541 gtcgtctctt gctacgcgag aaccgaggag acgagagcgg ccttcgccga ggagcacgga
      601 tgccgcgcgg cgggcgacct cggcgagctg ctcgacgatc ccgaggtcga cggggtgatc
      661 gtggctaccc cgcattcgac gcacgcggac atcgcggtgg ccgcggccga ggcaggcaag
      721 cacatgttcg tggagaagcc cctcacgctg acggtcgccg atgcgaagcg ggtgggagac
      781 gcagcctcgc gagcgggggt gacggtccag gtcggacaca atcggcgccg ccagccggcc
      841 aatcgcaaga tcaaggcgat gatcgacgcc ggcgagctcg gcaccgtcct tcagctcgag
      901 ggcatgcact cggccgccgg tggtcacaag cccgatctcc cggcgtggcg gaaagatccc
      961 gccgagtgcc ccttcggcgg tatgacggcg ctcggagtcc acacggtgga caccttccac
     1021 tacttcatgg ggcccgggaa gagggtgacg gcgctcagca cccggttccg cgggttcaac
     1081 gacctcgacg acgcgacgac cgttctgatc gagtacgagt cgggtccgat cgcgtcgatc
     1141 aacacgacgt acttcgctcc gccggtcgtc tcgctctccg tgttcggcag cgacgccgcg
     1201 gcgtggaacg aggaggacgg cacgcgtctg tacacacagg tccggagcga ccccgcgcgc
     1261 accgagcatg aggtcgaaac catcgacacg atgcacgacg agatcgtcga gttcgcacgc
     1321 tgcatcggtg gcgaggccga gccggagacc ggcatgcccg aggcgatcga ggtcgccgcg
     1381 gtcctggagg cgatcggaag gagcgttgcg tccgggagcg tcgtcgagct ggccgatctc
     1441 cgctagacga tcacgccggc cggcgtgtcc tcgtcccgtt cggcgcgcag ggcaagagcg
     1501 gagtggatcg tcacggcgcc gaccaccagg actccgccgg ccagcacctc agccgaggga
     1561 cgctcgccga tcacgagcca gacccagagc ggcccgagga gcgtctcgag cagcgcgacg
     1621 aggttcacct ccggcgcagg gacgtgacgg gtcgcgcggg tgatcagcgt gagcgagatc
     1681 ggaaggatca cgaccccgag gagcgcgaga aggagcatgt cgctcgcatc gatggagacg
     1741 tcagcgccgg cgatcccccc cgcgatcgcc gctcccaccc cgcctatcgc gatcgccggc
     1801 gtcatgtcgg cgggccgcgc cgctcgggag accgtcaggt agccggcgaa gcaagtggcg
     1861 ccggccagcg cgagcaggtc gccgtcgagg tcgccctgcc cgatactccc gccgaggatc
     1921 agtgagaggc ctgccaccac cgcgatcccc gtcagccacg tcctcgcggc cgcccgctgg
     1981 cggaggaaga cacgtcccag gaccgacgcg atcaacggac ccgcggcgat gatcaccaac
     2041 acgctcgccg catccgtccg atcgagcgcc gagacgaacg cgatgctcgc cgccgcgaag
     2101 agcgcaccgg cgaacacgtg gggcccggtg ggtcgccatg agcggtgacc atcagccgga
     2161 cgcaggaggc ggagcagcgc gacggtgccg acgacggtga agatccctcg ccacgccacc
     2221 gtggtccacg cgttcgcttc caccgaccgg acgatcaggg cgtcggggct gatcacgacg
     2281 acgccgatcg cacacagcgc gaggccgcgg aggtgatctc gacgaccact cgtgtcgctc
     2341 atggatccca cctggccgtc ggaccccttc tgcctggaca acttatcgca tattctatag
     2401 agtgcgtcac ctgatcgggc ccgatagcac ggggaggggg ctccacatgg ctggacgcag
     2461 ggtgcttcgc ggtatcgcgg tccttgctgc cgcaacgctg ttcgcggtgt cgtgcggcga
     2521 cgacggtggc ggcggaggtg gcggcggtgg cggaggcgaa gagagcaaca tcatccggtt
     2581 cacgttcgcg ccggacccgg cctgggactg gatgaaggac caggggatcc tcgaggcgat
     2641 ggagcaggag tccggattcc ggatcatcca gctcgccacg tgggacgagt gggcgacgtt
     2701 cgccgggggt catgccgacg tcgtctcgac gggcacgtac gagactccgc tcctcgagga
     2761 gcagggcatc gagaccgtca cgttcggcca gttcaatatg aacaaggacg tgctcgtgac
     2821 ggcgcacccc gagtaccaga cggcatccga cttcccggcc ggctgcaaga tcgcgacgga
     2881 gtccgtgacc gggaacacga tcatctgggg gtcgctgatc aacgcgatcg acggccgcga
     2941 ggtcgcggag gattccgatg acatcgggat cgtcacggcc gactaccagg tgatcccgaa
     3001 cctgctccgc gaggacgagg cgtgcgccgg gatcctcgac ccgacccagg tgatcccgga
     3061 catggcatcg ggggatctca cggtgatgta cgacgggaag tccgcgtcgc agctctacgg
     3121 cgaggagatc gtgcctggtc acgagggtgt gctcagcaac gcgttcgtgt cccgagccga
     3181 atggttcgac gcgaatcccg aagcggcggc cttcttccag gaggtgtggg actgcgcgat
     3241 ggtccagtgg gccgagaacc gcgacgagat catcgatacg tacccccagc acttcgcggt
     3301 cgagaccgag gccgaggcgc agttcatgaa ggactacttc gagaacacct tcgactggtt
     3361 cgtcgagagc ccctacctga ccgaggagtg gatcgagggc gagcgcccgg tcttcgacct
     3421 cgtccaggac gctgaagtca ttccggcgga cgcggagttc cctcggcacg aagtgatgga
     3481 ggatccggaa ggcgagccct gtccggaggg cttctagcga gcgatccggt gccggcgtcg
     3541 cgtcagtgac gccggcaccg ccctctcccc tcatggccgc gaccgtcgag accgagaaga
     3601 ccctcgagga gctcgagcag gaggcgatcg atgcgctcgc ctcgagcggc cggcgcacgc
     3661 gcgcgaaggt cctctactcc ctgctcggcg cggccagctt catcgccgtc tgggcgtact
     3721 ggtcaacggt cctcgagccg ttcatcctgc cgtcgccgct cgcggtggcg cagcggatgt
     3781 gggagctcct cgtctcgggc gtcgtcttca cgaacttcct gagcagcttc ctgaagacga
     3841 tggccggctg ggcgatggcc ctcatcatcg ggatcccggt ggggatgttg atgggccgct
     3901 accgctacgc ccgcgcgttc ttccacgact tcgtctacct gttcgcgaac gtgccgctcc
     3961 tcgtgtacgc ggtgatcgcg atcgtcgtgt tcgggatcag cccgtggggg ccggcgttcg
     4021 tggtggcgct tgaggcgttc accggcatcg cgatcaacgt cgcggccggc gtggaatcgg
     4081 tggatcgcgg gctcctcgcg atgagccggt cgttccggcg gagcagccgc aggacgctcc
     4141 ggagcatcgt ggtcccctcg gtggtgccgt tcctcttcgc gagcggccgc gtctcgttcg
     4201 cgaactcgtg gaagctggcc gccctggcgg agacgttcgg cggctacctg ggggtcgggt
     4261 accagctcga gaagtcgttc cagatctact cggtcgtcga cctgctcgcg tggatgttct
     4321 tcttcgtcct gttcgtgatc ctggtcgaac ggctcctgat cgcgccggcg gagcgacggg
     4381 tgttccgctg gcgcgacccc gcgggagcca gggagcaggt gtcgtgagcg acgtggcgac
     4441 cgtcgacggc ctcggatcgg ccgagatccc ggagttcctg ctcgctccac cgccgcggtc
     4501 tcggtggtgg tggctcacct cggatcgcgc cgcgcggtac acggcgcgcg tggcgttcct
     4561 gctcgtctgg caatgggccg ggtcgacgtt cgaggacatc ccgacgcccc tcggcaccgt
     4621 cgaggccctg atcgaggagt tccaacgggg cgaggtcttc cccaacctgg cgatcagcct
     4681 ggtccgggcc gtgatcggac tctcgatcgt cctcgtcctc ggggtgtcga tcgggatcgc
     4741 gatggcccgg tggtggcgcg tccgctactt cttcaccgat ctgatcctcg tgggcatcac
     4801 gctgccggcc ttcatctggg cgctcctggt cgtgatgtgg tgggggttca gcaatatcgg
     4861 gccgatcacc gtctgtgtgc tgtcggcgac gccccagctg gtcgtcaaca cgttccaggg
     4921 cgcgaccgca acccccggga ggctgctcgc gatgtctgac gcctaccggg taggcactcg
     4981 gcggcagttc cgatggctca tgatcccgtc gatgatggag tacatcgccg ccggcttccg
     5041 ctcggccgtc ctggcgggat ggggagccgt cctgttggtg gagtggttcg gcaacgacag
     5101 aggggtcggt ttccgcgccc actactggta cgacgcccgc agcttcgagg gcatgatggc
     5161 ctggggtctc gtgatgatga tcacgatcct cgccttcgat cgcgtcgtga tggaccgggt
     5221 cgtccgtcgc ttccgtggct ggcggaccgg tggtcagcag tggtctcgat gaccgttcgc
     5281 cggacggtac ggccgccggc cggaggtgat ccgacgtggc aagactggta gcgcgagatg
     5341 tgaagaaggt gttcccgccg acgcgggcgg gccaggatcc ggtgttcgcg ctccgcaacg
     5401 tgagcttcga ggcggaggga cacacgttcg tgacgctcgt cgggccgtcc ggatgcggga
     5461 agagcacgtt cctgaacatg gtctcgggga tcgagacacc gagcgcgggg accctcgagg
     5521 tgatcggtcg gagcgagggt gacgcccgga tcgggtacgt cttccaggat ccgcgattgc
     5581 tcccctggaa gaccgtgctc gacaacatgg tgtacgtcct cgacgagggg tcgtccgagg
     5641 agcgcaccga acgcggccgg aagtacctcg acctcgtgaa cctcggcgcg aaggccgaga
     5701 tgtttccggg acagctctcc ggcgggatgc agcagcgggt cggtatcgcg cgagcgctct
     5761 cgatcgaacc ggacctgctc ctgatggacg agccgttcag ccacctcgac gcgatcaccg
     5821 cgcgcgacct ccgcgtggag ctgcagcgcc tctggctgga cacgggctcc acgatcctgt
     5881 tcgtcacgca cgacgtgctg gaagccgtcc tgctctccag ccgggtgctg atgatgagca
     5941 gccagggggt gatcttccgc gatctcacga tcgacttgcc gttcccgcgc aggcagacgg
     6001 accgggaagt c
//
LOCUS       DASUVE010000161         2958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_459157, whole genome shotgun sequence.
ACCESSION   DASUVE010000161 DASUVE010000000
VERSION     DASUVE010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459157"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..814
                     /locus_tag="VF036_03425"
     CDS             443..814
                     /locus_tag="VF036_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264313.1"
                     /translation="MKSTGDPLAFATPEQWRAWLEANHASAEEAWVLISKKHGGAPLV
                     SLVEATEEALCFGWIDSAMRPIDTERFALRYTPRRQASKWSPTNRARVERLIDEGRMT
                     EAGLAAIEEAKRNGRWDDEVD"
     gene            915..1283
                     /locus_tag="VF036_03430"
     CDS             915..1283
                     /locus_tag="VF036_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6264314.1"
                     /translation="MNFNSVLIGSENPQPLAEYYTKLFGEPTWNDGGYVGWMIGSGAV
                     TIGPHDQVKGKNAEPGRVIWNIETADVKSEFDRFVEAGATVVQEPYSPGEEMDGQIAT
                     FADPDGNYFQLMSPMSPEGS"
     gene            1307..2848
                     /locus_tag="VF036_03435"
     CDS             1307..2848
                     /locus_tag="VF036_03435"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX6264315.1"
                     /translation="MLLGEVAAASADVAATSSRLEKVARLSSVLAALEPVEVPIAVAY
                     LSGVLPQGSIGVGWASLKDLPAATPEPATLALLDVDAALSRIGALAGAGSQAARRTAL
                     HALFARATDDEQDFLRGLLMGEIRQGALEGAMVEAIARAANVPRTEVRRAAMLTGDLG
                     ATATAAIDGGSAALAGFRLSLLTPLQPMLAQSADDVPSALARISPAAVEWKLDGARIQ
                     VHRQGDEVRAFTRNLADVTDRVPEIVAVIRSQPLEAAVFDAEVIALRPDRRPHAFGLT
                     MSRFGARSRKDLTEFQTDYPLSAFVFDVLHLDGEDLIDRPAVERFAALDERLPDELRV
                     PRIVTADVEEADRFLDDAIARGHEGVMVKALDAPYEAGRRGAGWIKVKRAHTLDLVVL
                     AAEWGHGRRRGRLSNLHLGARDPETGGFVMLGKTFKGMTDEMLAWQTERFQEIETGRE
                     GITVHVRPELVVEVAFDGLQPSPRYPGGLALRFARVKGYRPDKRADDADTIETVRAIH
                     ERSAS"
     gene            2860..>2958
                     /locus_tag="VF036_03440"
     CDS             2860..>2958
                     /locus_tag="VF036_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6264316.1"
                     /translation="MPKLSAGLLAYRRTDAGVEILLVHPGGPFWAKK"
BASE COUNT          461 a          994 c         1095 g          408 t
ORIGIN      
        1 cgttcacgtg ggtgtcgagg tcgagcaggc cgtcttcgat cgcttggagc acggccgtgg
       61 cgacgacggt cttggagacg ctcgcgagca tgaacagcgt gtcgggcgtg acgcgccgtc
      121 ccgctgcgat gttcgccatc ccccacgcgt gcgaaaccag acgacctccc ctccggggac
      181 gacggccgca ctgagtccgg ggagccgccc ggcggccatc agctcgcgga tcccgcggtc
      241 gaggtcggcc ggcaccgcgc cgcgcgcccg caaccgcggt gcgagcacga ccgccgcccc
      301 gacggctccg ccgatcagca cgtcgcgtcg aagcacgcac ggtgagcgga acggggggtc
      361 cgtcgccatg cgccgatggt gacatgacct catcgagccc ctcgaggggt cgtcttgaga
      421 gggcgtgcct aggatggcgg acgtgaagag caccggcgat ccgctcgcgt tcgccactcc
      481 tgagcagtgg cgcgcgtggc tcgaggccaa tcacgcctcc gccgaggagg cgtgggtgct
      541 gatctccaag aagcacggcg gggcgccgct ggtctcgctc gtggaggcga ccgaggaggc
      601 gttgtgcttc ggatggatcg acagcgcgat gcgtccgatc gacacggagc gattcgcctt
      661 gcgctacacc ccgcgacgac aggcgagcaa gtggtcgccg accaaccgcg cccgggtgga
      721 gcggttgatc gatgaggggc ggatgaccga ggccggcctc gctgcgatcg aggaagcgaa
      781 gcggaacggg cgctgggacg acgaggtgga ctgaagacgg ccctgaagcg tcgaacggga
      841 cgcgatcccc tcgatgcgtg tcccatgcgc cgtccgtctg gtagtcaaga ggcgagggaa
      901 gggagggatg cgtcatgaac ttcaacagcg tcctgatcgg gtcggagaac ccgcagccgt
      961 tggccgagta ctacacgaag ctcttcggcg agccgacctg gaacgacggc ggctatgtcg
     1021 gctggatgat cgggtcgggt gccgtcacga tcggccctca cgatcaggtg aaggggaaga
     1081 acgccgagcc gggcagggtg atctggaaca tcgagaccgc cgacgtgaag tccgagttcg
     1141 accggttcgt ggaggcggga gccaccgtgg tccaagagcc ctacagcccg ggggaggaga
     1201 tggacgggca gatcgccacg ttcgccgatc ctgacgggaa ctacttccag ctcatgagcc
     1261 cgatgagccc agagggctcg tagcggccac aggctcgaag gcacggatgc tcctgggtga
     1321 ggtcgcggcg gcgtcggcag acgtcgccgc gacctcgtcc cggctcgaga aggtcgcccg
     1381 gctctcgtcg gtcctcgcgg ccctcgagcc cgtcgaggtc ccgatcgcgg tcgcgtacct
     1441 ctccggcgtg ctcccgcagg ggtcgatcgg cgtgggatgg gcatccctga aggacctgcc
     1501 ggcggcgacc cccgaaccgg ccacgttggc attgctcgac gtcgacgccg cgctctcccg
     1561 gatcggtgcg ctggcaggcg ccggctccca ggccgcccgg cgcaccgcgc tccacgctct
     1621 gttcgcgcgg gccacggacg acgagcagga cttcctccga ggactcctga tgggcgagat
     1681 ccggcagggg gccctcgaag gggcgatggt cgaggccatc gcgcgagccg cgaacgtccc
     1741 gcgaacggag gtccggcgcg ccgcgatgct gaccggagac ctgggcgcga cggcgacggc
     1801 ggccatcgac ggagggagcg ccgccctcgc aggcttccgg ctctcgctcc tcaccccgct
     1861 gcagccgatg ctcgcgcagt ccgccgatga cgtcccctcg gccctcgcgc ggatctcgcc
     1921 ggcggcggtc gagtggaagc tcgacggcgc ccggatccag gtgcaccggc agggggacga
     1981 ggtccgcgcg ttcacacgga acctcgccga cgtgaccgat cgcgtgccgg agatcgtggc
     2041 ggtgatccgg agccagcccc tcgaggccgc ggtcttcgac gctgaggtga tcgcgctgcg
     2101 acccgaccga cggccacatg ccttcggcct gacgatgagc cggttcgggg cccggagccg
     2161 gaaggacctc acggagttcc agacggacta cccgctgtcc gcattcgtct tcgacgtgtt
     2221 gcacctcgac ggcgaggacc tgatcgaccg ccccgccgtc gagcggttcg ctgccctgga
     2281 cgaacggctg cccgatgagc tccgcgttcc tcggatcgtc acggcggacg tcgaggaggc
     2341 cgaccgcttc ctggacgacg cgatcgcgcg tgggcacgag ggcgtgatgg tgaaggcgct
     2401 cgacgctccc tacgaggcgg gtcgccgggg cgccggctgg atcaaggtga aacgcgcgca
     2461 caccctcgac ctcgtggtcc tcgcggccga gtggggacac ggacggcgac ggggccggct
     2521 gagcaacctt cacctcgggg cgcgggatcc cgagaccggt gggttcgtga tgctcgggaa
     2581 gacgttcaag gggatgacgg acgagatgct cgcgtggcag acggagcggt tccaggagat
     2641 cgagaccggt cgcgaaggga tcaccgtgca cgtccggccc gagctcgtcg tcgaggtcgc
     2701 gttcgacggc ctgcagccga gcccgcgcta ccccggcggc ctggcgctgc gcttcgcccg
     2761 cgtcaagggc taccggcccg acaagcgggc cgacgacgcc gacacgatcg agacggtccg
     2821 ggcgatccac gagcggtcgg cgagctgagg aggcgcgccg tgccgaagct gagcgccggg
     2881 ctcctcgcgt accggcgcac cgatgcgggg gtggagatcc tcctcgtcca cccgggcggt
     2941 ccgttctggg ctaagaag
//
LOCUS       DASUVE010000162         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_834027, whole genome shotgun sequence.
ACCESSION   DASUVE010000162 DASUVE010000000
VERSION     DASUVE010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834027"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..2318)
                     /locus_tag="VF036_03445"
     CDS             complement(3..2318)
                     /locus_tag="VF036_03445"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX6264317.1"
                     /translation="MVDKGARRTKRSPRVDSIVERLAGKRVFMTGATGFLGQVVLERL
                     LADFPETTVVLLVRSQTGASSVERVHYLTRKPSFDRLRESLGSDDALLRLLDERIEVI
                     DGDFSRGRPDLPGAIDVVIHSAATVSFDPPIDEGFQTNLLGAMNLYGAVLDGGSRPAL
                     VHVSTAYVAGVQKGVIPERTLDHRVDWRLEADLAMSARRDVEAASRKPELLERFLEDA
                     SQEHSRAGPTTVADDAEQRRKEWMTKRLVEYGRQRARSLGWPDVYTFTKAMGERAVEE
                     LAAAEQLPLSIVRPSIIESALLHPAPGWIDGFKMADPIIRAYGLGQIPEFPGIPEGIV
                     DIIPVDFVVNAILAVAANPPTPGEPAHYHVSSGDRNPLQFHQLYALVREYFEAHPLPE
                     RGRGEHKVPEWTFPGNVTVEKMLRRAERLTDVAEQVVTHLPKSKGMRDAVRRVDRDKA
                     RVDFVKRYSDLYGAYTEAEVIYTDDVAMQLFQSLATEDRARFPFDAAMIDWKHYLQDV
                     HSPAVTQSLRELARKDRARPAVKIREREEPVLAVFDMEGTIISSNVVESYVWARMADL
                     EPDGWPGELVSVFGRIPRYLAIDRRDRGDFLRTFFRRYEGASVEGLERLVDRHVGEFM
                     LQKASPGAIRRIREHRAAGHRTLLLTAAANAFLRPLQPLFDVVIGADLQVDAEGRYTG
                     FMSTPPLVGEARAAWLRRYAAGEAVDLKASYAYADSYSDLPLLRAVGNPVAVSPDSHL
                     YRYARRRRWPIEEWAMSKGMPRVRFPKPARR"
BASE COUNT          403 a          920 c          872 g          398 t
ORIGIN      
        1 ccctacctcc tcgccggctt cgggaatcgg acgcgtggca tgcccttcga catcgcccac
       61 tcctcgatcg gccacctccg tcgacgcgcg taccggtaca ggtgcgagtc gggtgacacg
      121 gcgacggggt tccccaccgc gcggagcagg ggcagatcgg agtaggagtc ggcgtatgcg
      181 taggaggcct tcaggtcgac ggcctcaccg gccgcgtagc ggcggagcca ggccgcccgc
      241 gcctcgccga caagtggcgg ggtggacatg aacccggtgt agcgcccttc ggcgtccacc
      301 tggaggtcgg ccccgatcac gacatcgaac aatggctgca gagggcggag gaacgcgttc
      361 gcggcggcgg tgagcaggag cgtccggtgc ccggcggccc ggtgctcccg gatccggcgg
      421 atcgccccgg ggctcgcctt ctggagcatg aactcgccga cgtgccggtc gacgagccgc
      481 tcgaggccct cgaccgacgc accctcgtac cgccggaaga acgtgcgcag gaaatcgccg
      541 cgatcacggc ggtcgatcgc gaggtaccgc gggatcctgc cgaagacgct caccagctcg
      601 cccggccatc cgtccggttc gaggtcggcc atcctcgccc acacgtacga ctccacgacg
      661 ttcgacgaga tgatcgtccc ctccatgtcg aagacagcga gcaccggctc ttcccgctcc
      721 cggatcttga ccgcgggccg agcgcggtcc ttccgtgcga gctcgcggag cgactgggtc
      781 acggccggcg aatggacgtc ctggaggtag tgcttccagt cgatcatcgc ggcgtcgaac
      841 gggaagcggg cccgatcctc cgtcgcgagg ctctggaaga gctgcatcgc gacgtcgtcc
      901 gtgtagatca cctcggcctc cgtgtacgcg ccgtagaggt ccgagtaccg cttcacgaag
      961 tcgacgcggg ccttgtcccg atccacgcgc cggacggcgt cccgcatccc cttcgacttg
     1021 gggagatggg tgacgacctg ctccgcgacg tcggtcagtc gctccgcccg gcggagcatc
     1081 ttctccacgg tcacgttccc cgggaacgtc cactccggga ccttgtgctc gccccggcca
     1141 cgttcgggga gcggatgcgc ctcgaagtac tcccggacga gcgcgtagag ctgatggaac
     1201 tggagcgggt tccgatcgcc cgaggacacg tggtagtgcg ccggctcccc cggcgtcgga
     1261 gggttcgcgg ccaccgcgag gatcgcgttc accacgaagt cgaccgggat gatgtcgacg
     1321 atcccctccg ggatgccggg gaactcgggg atctgtccga gcccgtacgc ccggatgatc
     1381 ggatccgcca tcttgaagcc gtcgatccac cccggcgccg ggtgcaggag cgcggactcg
     1441 atgatcgacg gccgcacgat cgacaacgga agctgctcgg cggcggcgag ctcctccacc
     1501 gcgcgctcgc ccatcgcctt cgtgaacgtg tacacgtccg gccatccgag ggaccgcgcg
     1561 cgctgccgcc cgtactcgac gagccgcttg gtcatccact ccttgcgccg ctgctcggcg
     1621 tcgtccgcga cggtcgttgg ccccgcgcgc gagtgctcct gggacgcatc ctcgaggaag
     1681 cgctcgagga gctcgggctt ccgcgaggcg gcctccacgt cacggcgagc cgacatcgcc
     1741 aggtcggctt cgagccgcca gtccacgcga tggtccagcg tgcgctcggg gatcaccccc
     1801 ttctgcaccc cggccacgta cgccgtcgag acgtggacga gcgcgggtcg cgatcctccg
     1861 tcgagcacgg cgccgtagag gttcatcgcg cccagcaggt tcgtctggaa gccctcgtcg
     1921 atgggcgggt cgaacgagac cgtcgcggcg gagtggatca cgacgtcgat ggctcccggc
     1981 aggtccggac gcccccggga gaagtccccg tcgatcacct cgatccgctc gtccaggagg
     2041 cggaggagcg cgtcgtccga gcccagggac tcgcggagcc tgtcgaacga gggcttccgg
     2101 gtcaggtagt ggacgcgctc cacggacgag gcgcccgtct gggacctgac caggagcacc
     2161 accgtcgtct cggggaagtc ggcgagcagt cgttcgagca cgacctgccc caggaacccc
     2221 gtcgcgccgg tcatgaagac ccgcttgccg gccaggcgtt cgacgatcga gtccactcgc
     2281 ggcgacctct tcgtcctccg agcgcctttg tctaccatcc ggcagattat cgctctctac
     2341 cgttcggtag acaacccctc tgctcaagga ggcgctcccg caggtcgaaa ggcaggagag
     2401 gtcggacggc ctgcaggaga gagcaccgtg tcccttcgtt cgaagatgct cgcgatgtcg
     2461 acgatccctc tgatcgtcct cgtcttcgcc gtgacctatg cggtgtccgc ccagcgaacc
     2521 gcggcgagaa cgaacgaaga cgtggaccgc accaccggtg tccgacgcga gctcgccgag
     2581 atccagcacg acc
//
LOCUS       DASUVE010000163         2590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_834656, whole genome shotgun sequence.
ACCESSION   DASUVE010000163 DASUVE010000000
VERSION     DASUVE010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834656"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VF036_03450"
     CDS             <1..498
                     /locus_tag="VF036_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6264318.1"
                     /translation="VLAVHSGTGFSGYVASDRVPGSIAAMVYVDTAPGIPPLDADFEG
                     DEKPLVWEEVSAEENLDGLSEDQLETVRHRAVPEPGGVLRDTVELTNDARLDLPSTIV
                     CTGYTSEQYKEAMGEGYAWLAGLQEVRNLIWVDLPTSHWPMWSRPKELAEIIGDVAKA
                     HASGG"
     gene            545..1300
                     /locus_tag="VF036_03455"
     CDS             545..1300
                     /locus_tag="VF036_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6264319.1"
                     /translation="MRDRSAAQYADFVLPAIGRGDQVLDVGCGPGSITLGIAEAAAHV
                     TGIDVDDAEFADGRAYAADHGIGNVEFREGTIYDLGVPEGSTDVCTLFAMLETLDDPL
                     AGLAEVRRVLKPGGVIGASSIEYGGLILHGADVLPLRRFYELRLALWEAQGDVHPYRG
                     RELRGLFLAAGFTQVEASTTYFSYGTEERVRTFGLGRAADCRDEWFVDGLTERGIADR
                     DEIDALEQTWIRWAEEPDSFAAFAWGRATARKP"
     gene            1371..2078
                     /gene="folE"
                     /locus_tag="VF036_03460"
     CDS             1371..2078
                     /gene="folE"
                     /locus_tag="VF036_03460"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEX6264320.1"
                     /translation="MRTDRETPDGQGSTARPADVAERWAMSDPEETTSWERVRPREVT
                     DEQWERFEGAVAEMFTAFGMDLDTEGTHNTPRRFVRAMYEATTGYEGDPKLLTAFPTE
                     CRGGPDCSISQVIEGPIPFFALCEHHAFPFYGVAHVGYVAHEQIIGISKMTRLVRVYA
                     RRFTVQERLGQQIADHLNDVMDPHGVAVHLQAAHLCTQMRGVREERSRTSTSFWRGNY
                     ERDPEMRREFLTVAQRE"
     gene            2075..>2590
                     /locus_tag="VF036_03465"
     CDS             2075..>2590
                     /locus_tag="VF036_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6264321.1"
                     /translation="MTALDVRLESVFETGGAPDVPLPDEVTSAFGGSFWLPERVVYAN
                     FVASIDGVAAIGGVEMSSAAISGGASADRFMMALLRAVADAVVVGAGTVREHGGPWTA
                     EKAYPPGAESFARLRAGISASESPTLVVVTASGELPGEHPALADAIVVTTSTGARSLA
                     QARVGEVIDVGD"
BASE COUNT          407 a          860 c          948 g          375 t
ORIGIN      
        1 gtgctcgccg tgcacagcgg gacaggcttc tcgggctacg tcgcgagcga tcgggtcccc
       61 gggtcgatcg cggcgatggt ctacgtcgac accgccccgg gcatcccgcc cctcgatgcc
      121 gacttcgagg gcgacgagaa gccgctggtg tgggaggagg tgtccgccga ggagaacctc
      181 gacgggctca gcgaggacca gctcgagacg gtccggcatc gggcggtccc ggagccgggc
      241 ggcgtcctcc gtgacaccgt cgagctgacg aacgacgcgc ggctcgacct cccgagcacg
      301 atcgtctgca ccgggtacac atccgagcag tacaaggagg cgatgggcga ggggtacgcc
      361 tggctcgccg gcctccagga ggtgcggaac ctcatctggg tcgacctgcc gacgagccac
      421 tggccgatgt ggtcgcgtcc caaggagctc gccgagatca tcggcgacgt cgcgaaagcg
      481 cacgcttccg gcggctgacc cacgcgaccg atcagagagc acggtcgtgg ggttcgagga
      541 tctgatgagg gacaggagcg ccgcgcagta cgcggacttc gtgctccccg cgatcggtcg
      601 aggtgatcag gtcctcgacg tggggtgcgg gccgggttcg atcacgctgg gcatcgcgga
      661 ggccgccgcg cacgtgaccg ggatcgacgt cgacgacgcg gagttcgcgg acgggcgcgc
      721 gtacgccgcc gatcacggga tcggcaacgt cgagttccgg gaaggcacca tctacgacct
      781 gggtgtcccg gaggggtcga ccgacgtctg cacgctcttc gcgatgctgg agacgctcga
      841 tgacccgctc gccggcctcg cggaggtccg tcgcgtgctg aagcccggcg gcgtgatcgg
      901 tgcctcgtcg atcgagtacg gagggctgat cctccacggt gcggacgtcc tcccgttgcg
      961 ccgattctac gaactccggc tcgcgctgtg ggaggcacag ggcgacgtcc acccctatcg
     1021 cggtcgggag ctccgcggtc tgttcctcgc ggcgggcttc acgcaggtcg aggcatccac
     1081 cacgtacttc agctacggga ccgaggaacg tgttcggacg ttcgggctcg gtcgcgccgc
     1141 cgattgccgg gacgagtggt tcgtggacgg actgaccgaa cgagggatcg ctgaccggga
     1201 cgagatcgac gcgttggagc agacctggat cagatgggcc gaggaaccag actcgttcgc
     1261 cgcgttcgcc tggggaagag ccaccgcacg gaagccctga gccaaacgcc cggcgggacc
     1321 gtacggtggg tggaggaacc cgaaccgacg gaacgaggcg gaggccgagg atgagaaccg
     1381 atcgagagac cccggacggg cagggctcga ccgcgagacc cgcggacgtc gcggagcgct
     1441 gggcgatgag cgacccggag gagaccacgt cgtgggagcg cgtgcgacct cgggaggtca
     1501 ccgacgagca gtgggagcgc ttcgagggcg cggtcgcgga gatgttcacc gccttcggca
     1561 tggacctcga caccgagggg acccacaaca cgccgcgccg gttcgtccga gcgatgtacg
     1621 aggcgaccac cggctacgag ggcgacccga agctcctgac cgccttcccc accgagtgtc
     1681 gcggcggacc cgactgctcg atcagtcagg tgatcgaggg accgatcccc ttcttcgcgc
     1741 tctgcgagca ccacgccttc ccgttctacg gcgtggcgca cgtgggatac gtggcccacg
     1801 agcagatcat cgggatctcg aagatgacga ggttggtccg ggtgtacgcg cgccgcttca
     1861 cggtgcagga acgcctcggc cagcagatcg ccgaccacct gaacgacgtg atggatccac
     1921 acggcgtcgc cgtacatctc caggccgcgc acctctgcac ccagatgcgg ggcgtccgcg
     1981 aggagcgttc gcggacctcc acctcgttct ggcgcggcaa ctacgagcgg gatcccgaga
     2041 tgcgccgcga gttcctcacc gtggcgcagc gcgagtgacc gcgctcgacg tccgtctgga
     2101 gtccgtcttc gagaccggcg gggctcccga cgtccctctc cccgacgaag tgacatcggc
     2161 cttcgggggc tcgttctggc tcccggagcg agtcgtctac gcgaacttcg ttgcgtcgat
     2221 cgacggcgtc gccgcgatcg gtggcgtcga gatgtcttcg gccgcgatca gcggcggcgc
     2281 ctcggccgac cgattcatga tggcgctcct gcgcgccgtg gcggacgccg tcgtggtcgg
     2341 cgcggggacg gttcgggagc acggcggtcc ctggaccgcc gagaaggcgt acccgccggg
     2401 cgccgagagc ttcgcccggc tgcgagccgg gatctcggcg agcgagtcgc cgacgctcgt
     2461 cgtcgtgacc gcgagcgggg aactcccggg ggagcatccg gccctggcgg acgcgatcgt
     2521 cgtcacgacg tcgaccggcg ctcgatccct cgcgcaggcc cgggtcggcg aggtgatcga
     2581 cgtcggggac
//
LOCUS       DASUVE010000164         3825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_835986, whole genome shotgun sequence.
ACCESSION   DASUVE010000164 DASUVE010000000
VERSION     DASUVE010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835986"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VF036_03470"
     CDS             complement(<1..958)
                     /locus_tag="VF036_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX6264322.1"
                     /translation="MALGSRDLSRVWLATSILGLATGGVLTWMGQPDAANVAWAITTA
                     VALVSLLRDVVTGLLRRQPGVDVIALLAMGGSLALEEYLAGAVIALMLASGEALEAFA
                     DRRAHRELSALLDRAPRTVSRYEGNDLVERPVGEVLRGDRLLVKPGAVIPVDGIASTH
                     AVLDEAALTGESRPVERAAGDPIRSGAVNAGGAFDLLATTTAEGSTYAGIVRLVREAE
                     RQKAPFVRLADRYAAIFVPITLVVAGVAWIAGDATRALSVLVVATPCPLLLAVPISIV
                     SGISRAAHRGIIVKGGGVLETLARAKVLVLDKTGTLTSGVPEV"
     gene            complement(961..1197)
                     /locus_tag="VF036_03475"
     CDS             complement(961..1197)
                     /locus_tag="VF036_03475"
                     /inference="COORDINATES: protein motif:HMM:NF020348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HEX6264323.1"
                     /translation="MDLTLAHPIGVAEVAEGTVEGTTVSLSSTAIIRTATGSPVTEIA
                     RRYHREGERLSYELDMAMDGVARTFHVRATLGRA"
     gene            complement(1511..1912)
                     /locus_tag="VF036_03480"
     CDS             complement(1511..1912)
                     /locus_tag="VF036_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264324.1"
                     /translation="MSEYQEEVLINLLDLQAELRGDESEQADADGQGAPEASAEPAAQ
                     EETLAVTDGGLTVSVLSPGDEAAAHDRLVALSDRVATLESRLAGVTERIERIEPAADA
                     HATGSPETDGDAASGSFLDLQKIVADRLDSR"
     gene            2028..2834
                     /locus_tag="VF036_03485"
     CDS             2028..2834
                     /locus_tag="VF036_03485"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX6264325.1"
                     /translation="MRTLVRLSVTPVKALRLSHPREADLTTGGIPTDRRFYLIDDAGA
                     LFDASDMGGLLRIVPDYDPSTERLRCTFPDGPVVEGTVDGLGATVTTDFFGRPVEGRL
                     VTDGFSEAFSSALGRSVRLVRVKRNGDGQDVHPLTIVSSASVRAIGTHGGRPDLDPRR
                     FRIQLEIDGCDPYEEDSWDGGLVRVGEATIRVRGQIPRCIVTTLGPDTGDKDFTTLNL
                     IARTRPRIEGRRGLPFGMYAEVVDPGHVRVGDPVEPVSARPTEATSVAGR"
     gene            complement(2791..2928)
                     /locus_tag="VF036_03490"
     CDS             complement(2791..2928)
                     /locus_tag="VF036_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264326.1"
                     /translation="MDVIVDILKFGLIFGVLGTAPIVTWLALSWLFTDRRRTSPPSDE
                     R"
     gene            3019..3468
                     /locus_tag="VF036_03495"
     CDS             3019..3468
                     /locus_tag="VF036_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264327.1"
                     /translation="MKPWVRWAILAAAVVVLVILFLLLRPDDGSDGATPTPSSPTVTE
                     TASPSDGVSPTPSESQAPERTIIEVTYRNGAVQGPTSFTVSQGERVRLLVHADVSDEV
                     HFHGYDLSADVTPQAAARIDFVANAAGRYELELEGAGEPLFELEIVP"
     gene            3498..>3825
                     /locus_tag="VF036_03500"
     CDS             3498..>3825
                     /locus_tag="VF036_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264328.1"
                     /translation="MATFLATAAPAFAHGPLAGRRDLGVPLGLVVFGAAAALIISFIA
                     LSALWTEPRFENRAQPRAKPSWLQSVLTNRLLEWVIRIAMLVVFLVVAMASARRAGPT
                     ETIGPIV"
BASE COUNT          542 a         1382 c         1329 g          572 t
ORIGIN      
        1 tgacctccgg gacgccggag gtgagcgtcc ccgtcttgtc caacacgagg accttcgccc
       61 gcgcgagcgt ctcgagcacg ccacctccct tcacgatgat cccccgatgc gcggcgcggg
      121 agatcccgga cacgatcgag atcggcaccg ccagcaggag cgggcacggc gtcgccacca
      181 cgaggaccga cagggctcgg gtcgcgtcgc ccgcgatcca cgccacgccg gcgacgacga
      241 gcgtgatcgg cacgaagatc gcggcgtacc gatcggcgag ccgcacgaac ggtgccttct
      301 ggcgctcggc ctcccggacg agtcgcacga tcccggcgta cgtgctcccc tcagccgtcg
      361 tcgtcgccag gaggtcgaac gcgccgccgg cgttcacggc accggaccgg atcggatccc
      421 cggcggcgcg ttcgaccggg cgcgactccc ccgtgagggc agcctcgtcc aggacggcgt
      481 gcgtcgaggc gatcccgtcg accgggatca cggcgcccgg cttcaccagc aagcggtcgc
      541 cgcgcagcac ctccccgacc ggacgctcga cgagatcgtt cccctcgtac cgcgacaccg
      601 ttcgtggggc gcgatcgagc agggccgaga gctcgcggtg cgcccgtcgg tccgcgaagg
      661 cctcgagcgc ctcgccggag gcgagcatca aggcgatcac cgcgccggcg aggtactcct
      721 cgagggcgag cgagcctccc atcgcgagca gcgcgatcac atcgacgccc ggctggcgtc
      781 gcagcagtcc ggtcacgacg tcccggagca gcgacacgag ggcgacggcg gtcgtgatgg
      841 cccacgcgac gttcgcggcg tcgggttggc ccatccacgt gaggacgccg ccggtcgcga
      901 ggcccagtat cgacgtcgcg agccaaacgc gagagaggtc ccgggagccg agcgccaccg
      961 tcaggctctc ccgagggtgg cacggacatg gaaggtgcgt gcgacgccgt ccatcgccat
     1021 gtccaactcg taggagagcc gctcgccttc ccggtggtac cgccgggcga tctcggtcac
     1081 cggtgaaccg gtggcggtgc ggatgatcgc ggtggacgac aggctcacgg tcgtcccctc
     1141 gacggtcccc tcggcgacct cggcgacccc gatcggatgc gcgagcgtga ggtccacgca
     1201 ccgtcgccca gcgggcgaag cgtacctcgc tcgaagtgca gcggcgctcc gtccggcgtc
     1261 cagctcgact cggtcaccag gaggaacgcg tcgccgacgt gctcgaacga gagctcctcc
     1321 gtatagcgga acggtgccac tccggggtag gtgccttccc cgtcaccgcg ccacgtcccg
     1381 agcaggaacg cgagcggttc gagcgcgacg ggaagctgga tagccatgcg cccgaagcta
     1441 tcacggggtc ccgtgccgac ctgcgtggaa ccgtggcgtg gaaccgtgcg gggtccggcg
     1501 gggccggatc tcagcgcgag tcgaggcgat ccgcgacgat cttctgcagg tcgaggaacg
     1561 accccgaggc cgcgtcgccg tcggtctcgg gcgagccggt ggcgtgcgcg tcagcagcgg
     1621 gctcgatccg ttcgatgcgc tccgtcacgc ccgcgaggcg ggactcgagc gtggcgacac
     1681 ggtcgctcag ggcgacgagc cgatcgtgcg cggccgcctc gtcgccgggg ctgaggaccg
     1741 agaccgtgag acccccatcc gtgacggcca gggtctcctc ctgagcggcg ggctcggccg
     1801 atgcctcggg agcgccttgc ccgtcggcgt cggcctgctc cgactcgtcg ccgcggagct
     1861 ccgcctggag gtcgaggagg ttgatgagga cctcttcctg gtattcgctc atccatctag
     1921 cctttcggtc tccggccgca gcgatctctc ttcgtccccc gtacgggtga tgctatgtca
     1981 caccgtgctc gccgaccaac ccgtctttcg gctacggttc tccgacggtg cgaacccttg
     2041 tccgtctgtc cgtgaccccg gtgaaagccc tacgcctttc gcatcctcgt gaggcggacc
     2101 tcaccacggg tgggatcccc acggaccgcc ggttctacct gatcgacgat gcgggcgccc
     2161 tgttcgacgc ctccgacatg gggggcctcc tccgcatcgt gcccgactac gacccctcca
     2221 ccgagcggct ccgctgcacg ttccccgacg ggcccgtggt ggaagggacg gtcgacgggc
     2281 tcggcgcgac cgtcaccacc gacttcttcg ggcgtcccgt tgagggtcgc ctcgtcaccg
     2341 atgggttctc cgaggcgttc tcgtccgccc tcggccgatc cgtccggctc gtccgcgtga
     2401 agcggaacgg cgacggacag gacgtgcatc ccctgacgat cgtgtcctcg gcgagcgtcc
     2461 gcgccatcgg gacgcacggc ggccgtccgg acctcgatcc gcgtcgtttc cggatccagc
     2521 tcgagatcga tggatgcgac ccgtacgagg aggactcttg ggacggcggt ctggtgcgcg
     2581 tcggcgaggc gacgatccgc gttcgcgggc agatcccgcg ttgcatcgtg accacgctcg
     2641 gccccgacac cggggacaag gacttcacga cgctgaacct gatcgcgcgc acccggccgc
     2701 ggatcgaggg gcggcggggg ttgccgttcg ggatgtacgc cgaggtggtc gacccaggcc
     2761 acgtgcgggt gggcgatccg gtggagccgg tcagcgctcg tccgacggag gcgacgtccg
     2821 tcgccggtcg gtgaacagcc acgagagcgc gagccacgtg acgatcggcg ccgtgccgag
     2881 gaccccgaag atgagcccga acttcaggat gtccacgatg acgtccaacc cgatgcctcc
     2941 gctcgtcggt cgcggacagc ctacctgagg ccgagcgcgc gaggctctct tcggatcgac
     3001 ctgcgatagc ctcgggcgat gaagccgtgg gtgcggtggg cgatcctcgc ggcagcggtc
     3061 gtggtgctgg tgatcctgtt cctcctcctc cgccccgacg acgggagcga cggcgccaca
     3121 cccacgccct cgagcccgac cgtcaccgag accgcctccc cgagcgacgg cgtgtctccc
     3181 acgccgtccg agagccaggc cccggagcgg acgatcatcg aggtgacgta tcggaacgga
     3241 gcggtgcagg gaccgacgtc cttcacggtg agccagggcg agcgggtccg gctcctcgtg
     3301 cacgccgacg tctcggacga ggtccacttc cacggctacg acctgtcggc cgacgtgacc
     3361 ccgcaggcgg cggcccggat cgacttcgtc gcgaacgccg ccggcaggta cgagctggag
     3421 ctggagggcg ccggcgagcc gctgttcgag ctggagatcg ttccctgatc aggcgaggga
     3481 cgatcctcgc cgcgctcgtg gcgacgttcc tcgccaccgc cgcccctgcg ttcgcccacg
     3541 ggccgctggc cggtcggcgc gatctcgggg tcccgctcgg gcttgtcgtc ttcggtgccg
     3601 cagccgcgct gatcatctcg ttcatcgcgc tctcggcgct gtggaccgag ccccggttcg
     3661 agaaccgcgc ccaacctcgt gccaagccct catggctcca gtctgtactc accaaccggt
     3721 tgctcgagtg ggtgatccgc atcgcgatgc tcgtggtctt cctcgtcgtg gcgatggcgt
     3781 cggcccggcg cgcgggcccc accgagacga tcggtccgat cgtcg
//
LOCUS       DASUVE010000165         6566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_461994, whole genome shotgun sequence.
ACCESSION   DASUVE010000165 DASUVE010000000
VERSION     DASUVE010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461994"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VF036_03505"
     CDS             complement(<1..882)
                     /locus_tag="VF036_03505"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX6264329.1"
                     /translation="MSRRLAIVWIAIVLLLGVLIALVPRAESAESTLALRRFLAASGR
                     QVSEGGPPPEAGGTYVILHDLRTPDEARSLLAWAASGGRLVVADPRSTLVTLSGASVG
                     DPVGLVGARSLEPACLAEEALGVRSIVVRASDRSLRGGDRFVSCFGGHLLVRSHDRGT
                     LVLLGGFTALTNEHLRTADNALLALRLAGSEGPVVFGPPVPAEVAAPATGVWGALPER
                     AKVAILAIAVAALAFAALRSRRLGRPPAEEPIAPIPASELVRATSRLYRRARSLGHAA
                     GMLREATRARVGRRLGVP"
     gene            complement(879..1766)
                     /locus_tag="VF036_03510"
     CDS             complement(879..1766)
                     /locus_tag="VF036_03510"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX6264330.1"
                     /translation="MGLLLAVPAGAQPRRATSPEELLDRLEAAAELADEGAADPSPDR
                     MRHVEEAMGLPAVVRIEGVDMAVPADPVMASMTGSSAGDFRRAADRLEALQGSLERAT
                     SAAPRDPDQIESALTNAYRGVLQVDPGIIERIRRAIGELIQGLLARLFSFQGAGTIVA
                     WAVLVGLGILAVWLIRRLRLVPETSMEVAGGGTGTPRVDWIARAEDAVRAGDLHLAVH
                     AFYRGLLGALSGRGLLIDGPGLTAGECRSTVRAVRPELFDAVADATGAFEQIAYGGAV
                     PEPGDVDTLRRAVALARSR"
     gene            complement(1799..2569)
                     /locus_tag="VF036_03515"
     CDS             complement(1799..2569)
                     /locus_tag="VF036_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264331.1"
                     /translation="MGVGELIDAAIKLYRSRWKTLMAIVAIALVPITFLQVFLTRQIG
                     SPFTEPTLTRAELESTVLTSIVLGAIQFLVVQPFLIAAVAKASADVYLGRPVLVGTTF
                     RFAVSRIHSILWISILSGLAILLGFVLLIVPGVILLVRFYFGTTVLVVEGQKGSKALG
                     RSFRLAQGHFWKILGTILLAAILASVVEGVLSIPGSAAFAAIGTAGWPFYAIGLSLAA
                     ILTTPFTTLISVLLYFDLRIRKEAFDLEIMAQEMSSQQ"
     gene            complement(2793..2878)
                     /locus_tag="VF036_03520"
     tRNA            complement(2793..2878)
                     /locus_tag="VF036_03520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2842..2844),aa:Leu,seq:taa)
     gene            2951..3382
                     /locus_tag="VF036_03525"
     CDS             2951..3382
                     /locus_tag="VF036_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX6264332.1"
                     /translation="MRNERGFTLIELMTVILIIGILVLLALPTFMGARQRASDRAALA
                     DIRTAFAAERTYYTDTLTYTTDPVEMDAIESALTYVDGDTPVATHVAYLHHHPLTNTL
                     FISAMSSLGTCWYLREIDGGGPQFASDPACGVADVQVYSTL"
     gene            3487..4434
                     /gene="add"
                     /locus_tag="VF036_03530"
     CDS             3487..4434
                     /gene="add"
                     /locus_tag="VF036_03530"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX6264333.1"
                     /translation="MRVETVRELAERVGAAVPSGLVDGRWGFAGSDDFISQYTAMCAL
                     LGTTEDFRRITYEFCEDEARTGVRYAEVVFSPSNHASRLGDDWFGPIEAVLDGLEAGR
                     RDFGVTAMLAPDLVRDAGIELAERSLEVALKFAGRGVVALNAAGSERSGIAPFGPLFR
                     RAKDGGLRSVPHAGEWAGPRNVWETLDHYLPDRIGHGVRAVDDPALVEHLAAAGIPLE
                     ISPVSNVATGVYSSLAEHPFRVLRDAGVTVTLNSDDPSMFGAWLTDVYRSVRDTWSFD
                     DADLAEIARTGVRASFADDDLKAELLAGVDAWLTEPPAA"
     gene            complement(4448..5329)
                     /locus_tag="VF036_03535"
     CDS             complement(4448..5329)
                     /locus_tag="VF036_03535"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6264334.1"
                     /translation="MSKRSRDRQLAKLAARRQAERTAALRRRNLIVGILAGVVALVVV
                     VVGLGVLLGDDPETAAPTPTPASPSGSPSPSVEPGTKTGTVTPTPATESGEVACGADE
                     PAKAGKPKPQFAGPPPMDIDRKATYTATMQTSCGAIVIELDPKRAPQTVNSFVFLAEE
                     VYFDGQYFHRLDTSIDVIQGGDPSGDGTGGPGYAIPDELTGDESYTPGTLAMANAGAN
                     SGGSQFFLITGPAGTNLDGNPAFTIFGKVIEGLDVAQRIQKLPIVDPDSGDISGQRPA
                     EAVYIESVTIKKSGGGG"
     gene            complement(5342..>6566)
                     /locus_tag="VF036_03540"
     CDS             complement(5342..>6566)
                     /locus_tag="VF036_03540"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEX6264335.1"
                     /translation="FWLTCPASVKSVSRLESDGWIARLADDLELEERIEEAHAAYASE
                     RGRMATGAERWGGVGGTGHGLKCLHAHYAYHLAGGDDAVGQWTAEQVEPVHGPEQGGR
                     VAAIDQGTNSTRLLVLQPGDGEPIEISRDMVITRLGKGVDEKGTLDPAAIERATEAIA
                     RFRRRARALHAEEFRIGATSAVRDALDREVFLSRVRDVTGIEPEVIEGEREAALSFLG
                     GTRGLDPADGPLLLIDIGGGSTEFVFGRKPGVVEDSISTQMGSVRLTERVQPSDPPTG
                     DDLQRFEELVLAQLDDVGDTVPVAEGRTLVAVAGTATTLQACALGLERYDPDLIHRSM
                     LTLPAAERTLGELATMTNEERAAIPVMPKGRGDVIVAGASILVHAMRRFGFEQALVSE
                     TDILDGLAFELLGVR"
BASE COUNT         1007 a         2337 c         2285 g          937 t
ORIGIN      
        1 ggggacgccg agtcggcgac ccactcgagc ccgcgtcgcc tcacgcaaca tgcccgcggc
       61 gtggccgagg ctccgagcac gccggtagag acgcgaggtg gcccgcacca gctcgctggc
      121 ggggatcggc gcgatcggtt cctcggcggg aggccgtccg agccgacgcg atcgcagcgc
      181 cgcgaacgcg agggccgcga cggcgatcgc gaggatcgcc accttggccc gctcgggcag
      241 cgcgccccac acgccggtcg ccggtgccgc cacctcggcg gggaccggtg ggccgaacac
      301 caccggaccc tccgatccgg caaggcgcag agccagaagc gcattgtccg ccgtgcggag
      361 gtgctcgttc gtgagcgccg tgaacccccc gagcaggacg agcgtgcccc gatcgtgcga
      421 tcggacgagg aggtgcccgc cgaagcacga cacgaaccgg tcgcccccgc ggagcgaccg
      481 atccgatgcc cgtaccacga tcgaccgcac ccccagcgcc tcctccgcga ggcacgcggg
      541 ttccagcgac cgtgcgccga ccaggccgac cggatcgccc acggaggctc ccgagagcgt
      601 cacgagggtc gaccgaggat cggcgacgac caatcgaccg ccggacgcgg cccaggcgag
      661 gagtgaccgg gcttcgtccg gcgtgcggag gtcgtggagg atcacgtacg tcccgcccgc
      721 ctcaggaggc gggcctcctt ccgagacctg cctccccgac gcggcgagga atcgccgcaa
      781 ggcaagcgtg gactccgcgg actccgcgcg cggtacaagg gcgatgagca cgcccaggag
      841 gaggacgatc gcgatccaga cgatcgccag ccgccggctc atctggacct cgccagcgcg
      901 accgctcgac ggagcgtgtc cacgtcgccc ggctcgggca cggcgccccc gtaggcgatc
      961 tgctcgaagg ctccggtcgc gtcggcgacg gcgtcgaaca actcgggacg gacggcgcgc
     1021 acggtggagc gacattcacc ggcggtcaga ccggggccgt cgatgagcag cccccggccc
     1081 gacaacgcac ccaggagccc tcggtagaag gcatggacgg cgaggtggag atcacccgct
     1141 cggaccgcat cctcggcgcg cgcgatccag tccacgcgcg gtgtgcccgt tccgccgcct
     1201 gccacctcca tcgacgtctc ggggacgagc cggagcctgc ggatgagcca aacggcgagg
     1261 atcccgaggc cgacgagaac ggcccaggcg acgatcgtgc cggccccctg gaacgagaag
     1321 aggcgggcga gcagaccctg gatcagctcc ccgatcgcgc gccggatccg ctcgatgatc
     1381 ccgggatcca cctggaggac accgcgatag gcgttggtga gcgccgactc gatctggtcg
     1441 gggtccctcg gagccgccga ggtcgcccgt tcgagggatc cctgcagcgc ttcgagccgg
     1501 tctgccgccc gacggaagtc gccggcgctc gagcctgtca tgctcgccat caccggatcg
     1561 gccgggaccg ccatgtccac tccctcgatc cggacgacgg cggggagacc catcgcctcc
     1621 tccacgtggc gcatccgatc cggtgacggg tcggcggccc cctcatccgc gagttccgcg
     1681 gcagcctcca accgatcgag gagctcctcg ggcgaggtcg ccctccgcgg ctgcgcgccc
     1741 gccggcaccg cgagcaggag gcccatcgca aggacgacgc acaggggcgc gatccgcctc
     1801 actgttgcga ggacatctcc tgggccatga tctcgaggtc gaacgcttcc ttccggatgc
     1861 gcaggtcgaa gtacaggagc acggagatca gtgtcgtgaa cggcgtggtg aggatcgcgg
     1921 cgagcgagag gccgatcgcg tagaagggcc atccggccgt gccgatcgcc gcgaacgccg
     1981 cgctcccggg gatcgacaga accccctcga cgacgctcgc gaggatcgct gcgagcagga
     2041 tcgtgccgag gatcttccag aagtgcccct gcgcgaggcg gaaggaccga ccgagcgcct
     2101 tcgacccttt ctgtccctcg acgacgagca cggtcgttcc gaagtagaag cgcaccagga
     2161 ggatgacgcc cgggacgatc aggagcacga acccgaggag gatggcgagt cccgacagga
     2221 tcgagatcca caggatcgag tggatccgcg agacggcgaa gcggaaggtc gttcccacga
     2281 gcaccggtct accgagatag acgtccgcgg acgccttcgc cacggcggcg atgaggaacg
     2341 gctggaccac gaggaactgg atcgcgccga ggacgatcga ggtgagcacc gtgctttcga
     2401 gctccgcccg ggtgagggtc ggctcggtga acgggctgcc gatctgccgc gtcaggaaga
     2461 cctggaggaa cgtgatggga acgagggcga tcgcgacgat cgccatcagg gtcttccatc
     2521 gcgagcggta gagcttgatg gccgcgtcga tgagctctcc gacacccatg ggttgcaggc
     2581 tgaacgggcg gtcccccggt gtccctccga gcggaggtgg cggcggggga aggggacgag
     2641 gcggacccca ggatgccgcg acggggggct gctggacggg tggctcgggg aggtttggcg
     2701 gcggctgacc gtccatgcac cggcatccta cgacggcctc gtccggcggc ggcgttccgg
     2761 tgccttcggc gggccgaagg accgccgttc cgtgcccggg gtgggattcg aacccacacg
     2821 ccccttccgg ggcaagcgct tttaaggcgc cgtcgtctac cgattccgac acccgggctc
     2881 ggtccgatgc tatcgctccg taggaaaccc ggggctcagg tcgctccccc ggagcgccga
     2941 tagttggcag gtgaggaacg aacgcggctt cacgctgatc gagctgatga cggtgatctt
     3001 gatcatcggc atcctggtgc tgctcgcgct cccgacgttc atgggtgccc ggcagcgggc
     3061 ctccgaccgg gcggcgctcg cggacatccg cacagcgttc gctgctgagc gcacctatta
     3121 cacggacacg ctcacctaca cgaccgaccc cgtggagatg gacgcgatcg agtcggcgct
     3181 cacgtacgtc gacggtgaca cgcctgtcgc cacccacgtg gcctacctcc accaccaccc
     3241 cctcacgaac accctcttca tctccgcgat gagctcgctc gggacctgct ggtacctgcg
     3301 agagatcgac ggcggcggcc cccagttcgc atccgatccc gcgtgcggcg tcgccgacgt
     3361 gcaggtgtat tccaccctct gagagcgcgt cgtcgcgcga ccgcgtccct ccccgctagc
     3421 atccgcggat gcgcgacctc gccctcctgc cgaaggcgga gctccacgtt cacctcgagg
     3481 ggcgcgatgc gcgtcgagac cgtccgcgag ctcgcggagc gagtgggcgc ggcggtcccg
     3541 tccggactcg tggacggccg gtgggggttc gcgggatccg acgacttcat ctcgcagtac
     3601 acggcgatgt gcgctctgct gggaacgacc gaggacttcc gacggatcac ctacgagttc
     3661 tgcgaggacg aggcccgcac cggcgtccgg tacgccgagg tcgtgttctc gccgagcaac
     3721 cacgcctccc gactcggcga cgactggttc gggccgatcg aggccgtgct ggacggactc
     3781 gaggcgggcc gacgcgactt cggtgtgacg gccatgctcg cgcccgatct cgtgcgcgat
     3841 gcgggcatcg agctggccga acggtcgctg gaggtcgcgt tgaagttcgc gggtcgaggc
     3901 gtggtggcgc tcaacgccgc ggggagcgag cggagcggga tcgctccgtt cgggcccctg
     3961 ttccggcgtg cgaaggacgg cgggctgcga tccgtgccgc acgccggtga gtgggcgggt
     4021 ccgcgcaacg tgtgggagac gctcgaccac tacctccccg accggatcgg acacggcgtg
     4081 cgcgccgtcg acgacccggc gctcgtcgag cacctggcgg ccgccggcat cccgctcgag
     4141 atctcgccgg tctcgaacgt cgcgaccggg gtctacagct ccctcgcgga gcacccgttc
     4201 cgcgtgctcc gggacgccgg agtgaccgtc accctcaact ccgatgaccc gtccatgttc
     4261 ggtgcgtggc tcaccgacgt ctatcggagc gtacgtgaca cctggagctt cgacgacgcc
     4321 gacctcgcgg agatcgctcg caccggggtg cgcgcatcgt tcgccgacga cgacctcaag
     4381 gcggagctcc tcgcgggggt cgacgcgtgg ctgacggaac cgcccgcggc ctgacggcgc
     4441 ctcagtttca gccgccgccg ccggacttct tgatcgtcac cgactcgatg tagacggctt
     4501 ccgcaggccg ctgccccgag atgtcgccgg aatcggggtc cacgatcgga agcttctgga
     4561 tccgctgagc gacgtcgagg ccctcgatca ccttcccgaa gatcgtgaag gccgggttcc
     4621 cgtcgaggtt ggtgccggcc ggaccggtga tgaggaagaa ctggctcccg cccgagttcg
     4681 cacccgcgtt cgccatcgcg agggtgccgg gcgtgtagga ctcgtccccc gtcagctcgt
     4741 cggggatcgc gtagccgggc cctccggtcc cgtcccccga cggatcgcca ccctggatca
     4801 cgtcgatcga tgtgtcgaga cgatggaagt actgcccgtc gaagtacacc tcctcggcga
     4861 ggaacacgaa ggagttgacg gtctgagggg cgcgcttcgg atcgagctcg atcacgatcg
     4921 cgccgcacga ggtctgcatc gtggcggtgt acgtcgcctt ccgatcgatg tccatcggcg
     4981 gcggaccggc gaactgcggc ttcggcttgc ccgccttggc cggttcgtcc gccccgcacg
     5041 cgacctcccc cgactcggtg gccggcgtgg gggtgacggt tcccgtcttc gtcccgggct
     5101 ccaccgaggg agacggactt cccgaggggc tcgccggcgt cggggtggga gcggccgtct
     5161 ccgggtcgtc accgagcagg acaccgagtc ccacgaccac cacgaccagg gccacgactc
     5221 cggccaggat cccgacgatc aggttgcggc ggcggagagc cgccgttcgc tcggcctgcc
     5281 gtctggcggc gagcttcgcg agctgacgat ccctgctcct cttcgacatc ggcggtcgag
     5341 gttaccggac gcccaagagc tcgaaggcga ggccgtcgag gatgtcggtc tcgctgacca
     5401 gggcttgctc gaaaccgaac cgccgcatcg cgtggacgag gatcgaggcc cccgcgacga
     5461 tcacgtcgcc acgacccttc ggcatgaccg gtatcgcggc gcgctcctcg ttcgtcatcg
     5521 tggcgagctc cccgagcgtg cgctccgcgg cggggagcgt gagcatcgag cgatggatca
     5581 ggtcggggtc gtagcgctcg agcccgagcg cgcacgcttg gagcgtcgtc gccgtgcccg
     5641 cgaccgccac gagcgtgcgg ccttccgcga cgggcaccgt atccccgacg tcgtcgagct
     5701 gcgcgaggac gagctcctcg aagcgctgca agtcgtcgcc ggtcggcgga tcggacggct
     5761 gcacccgttc ggtgagtcgc acgctcccca tctgcgtgga gatcgaatcc tccacgacac
     5821 cgggcttccg tccgaagacg aactccgtcg atccgccccc gatgtcgatc aggaggaggg
     5881 gaccgtcggc gggatcgagg cctcgggtcc ctccgaggaa cgagagcgcc gcctctcgtt
     5941 caccttcgat gacctcgggc tcgattcccg tcacgtcccg cacacgcgaa aggaagacct
     6001 cgcgatcgag cgcgtcgcgg acggccgacg tggccccgat ccggaactcc tccgcgtgca
     6061 gtgccctcgc acggcgccgg aaccgcgcga tcgcttccgt cgcccgctcg atcgccgccg
     6121 gatcgagcgt tcccttctcg tcgacgccct tccccagccg cgtgatgacc atgtcgcgag
     6181 agatctcgat cggttcgccg tcgccaggct gcagcacgag gagccgcgtg gagttggtcc
     6241 cctggtcgat cgccgcgacc ctcccccctt gctcgggccc gtgcacgggt tccacctgct
     6301 ccgccgtcca ctggcccacg gcgtcgtctc cgccggcgag gtggtaggcg tagtgcgcgt
     6361 ggaggcactt gagaccgtga ccggtcccac cgaccccgcc ccaccgctcg gcgccggtcg
     6421 ccatccggcc ccgctctgac gcgtaggcgg cgtgcgcctc ctcgatccgc tcctcgagct
     6481 cgaggtcgtc ggcgagacgc gcgatccagc cgtccgactc gagacgcgac acggacttca
     6541 ccgaggcggg gcaggtcaac cagaag
//
LOCUS       DASUVE010000166         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_272953, whole genome shotgun sequence.
ACCESSION   DASUVE010000166 DASUVE010000000
VERSION     DASUVE010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272953"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..95)
                     /locus_tag="VF036_03545"
     CDS             complement(<1..95)
                     /locus_tag="VF036_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX6264336.1"
                     /translation="MKVQNAVIPVAGLGTRFLPATKATPKEMLPVV"
     gene            167..538
                     /locus_tag="VF036_03550"
     CDS             167..538
                     /locus_tag="VF036_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020487542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX6264337.1"
                     /translation="MPTYEYTCRDCGHTFDIVQSMWDESLTMCPECGGSLRKVFAPPA
                     ISFKGSGFYATDHGKKSKASAESRSDGEKGEKKPGESTDGAKKTEGAASSDRSKDTSR
                     KGSSGGSEKSSTPAKKEPAPS"
     gene            535..1332
                     /locus_tag="VF036_03555"
     CDS             535..1332
                     /locus_tag="VF036_03555"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX6264338.1"
                     /translation="MTETRAEIGVIGGSGFYAFLEDTTTIEVETPFGAPSDPITIGTV
                     GDRMVAFLPRHGTDHRFPPHLVPYRANLWALKDLGVTRVFGPCAAGSLRKEIPPRTLV
                     VSDQAIDFTKSRSNTFYDGPQTTHVSFADPYCPTLRETLVKTAASQGIEHRDGGTMVV
                     IEGPRFSTVAESRMYAQLGADVIGMTQFPEATLARELELCYGNVALVTDYDVGVDDIP
                     AVTHEEVLKVFGENIDTLRDLLFAAIPAVPAERTCPCATALAGTGNG"
     gene            1329..1520
                     /locus_tag="VF036_03560"
     CDS             1329..1520
                     /locus_tag="VF036_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264339.1"
                     /translation="MTARRTLVTVEVRFVTDEDPEQLADRMREAVATIVGKQALEEFR
                     VKTLPLEPPNEKGGLRPVE"
     gene            1566..>2102
                     /gene="cpaB"
                     /locus_tag="VF036_03565"
     CDS             1566..>2102
                     /gene="cpaB"
                     /locus_tag="VF036_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX6264340.1"
                     /translation="MLFRRKLPRSSFILLSASVASALAAALVMRAYAHRLDLARPDGG
                     PPIAVVAAVAHVPRGSVLTETVLEVVTLPSRFAPPGAIRDPARATGRIVVADLAAGEI
                     VTRLRLAGSRSGPTASLVEAGMRAVEIPVASAVGVRPGDLVDVIATFGGGGAHTEVAG
                     EAIEVLAVDRAGGGASFGG"
BASE COUNT          323 a          733 c          749 g          297 t
ORIGIN      
        1 acgaccggca gcatctcctt gggggtcgcc ttcgtcgccg gcaggaaccg ggtgccgaga
       61 cccgccacgg ggatcacggc gttctgcacc ttcacgcgcg caacgctact ccggtcgcgg
      121 aaccgatgtc agccgcgaac aaggccccgg gtagacttgc ttcgggatgc ccacctacga
      181 gtacacgtgc cgggactgcg gccacacgtt cgacatcgtg cagtcgatgt gggacgagtc
      241 gctgacgatg tgccccgagt gcggcgggag cctccggaag gtgttcgcgc cgccggcgat
      301 ctcgttcaag ggctccggct tctacgccac ggaccacggg aagaagtcga aggcctcggc
      361 cgagtcacga tccgacggcg agaagggcga gaagaagccg ggagagagca cagacggcgc
      421 gaagaagacc gagggcgcgg cgtcgagcga ccggtcgaag gacacgagca ggaagggctc
      481 ctccggcgga tccgagaagt cgtccacgcc cgcgaagaag gagccggcgc cctcgtgacc
      541 gagacccggg cggagatcgg ggtcatcgga ggatcgggct tctacgcctt cctcgaggac
      601 acgacgacga tcgaggtgga gacaccgttc ggcgcgccgt ccgatccgat cacgatcggg
      661 acggtcggcg accgcatggt cgcgttcctc ccccgacacg ggacggacca tcggttcccg
      721 ccgcacctgg tcccgtaccg ggcgaacctg tgggcgctga aggacctcgg agtcacccgg
      781 gtgttcggac cgtgcgcggc agggtcgctc cgtaaggaga tccctccgcg cacgctcgtc
      841 gtgagcgacc aggccatcga cttcacgaag tcgcgctcga acacgttcta cgacggcccg
      901 cagaccacgc acgtctcctt cgccgacccg tactgcccta cgctccgtga gacgctggtg
      961 aagacggccg cctcccaagg catcgagcat cgcgacgggg ggacgatggt cgtgatcgag
     1021 ggcccgcggt tctcgacggt ggcggagtcg cggatgtacg cgcagctcgg cgccgacgtg
     1081 atcgggatga cgcagttccc ggaggcgacg ctcgcgaggg agctcgagct ctgctacggc
     1141 aacgtggcgc tcgtcaccga ctacgacgtc ggcgtggacg acatcccggc ggtcacccac
     1201 gaggaggtcc tgaaggtgtt cggcgagaac atcgacacgc tccgagacct cttgttcgcc
     1261 gcgatccccg cggtgccggc cgagcggacg tgcccgtgcg caaccgccct ggcgggcacc
     1321 ggcaacggat gacggcccgc cgaaccctcg tcaccgtcga ggtccggttc gtgaccgacg
     1381 aggatcccga gcaactcgcc gatcggatgc gggaggcggt cgcgaccatc gtcgggaagc
     1441 aggcgctcga ggagttccgc gtcaagacgc ttccgctcga gcccccgaac gagaagggcg
     1501 gtctgcgccc cgtcgagtga cagaggtcac tgtgtcgcgc ccggggcccg tctacggtcg
     1561 cgcccatgct cttccgccgg aagctgccgc gatcgtcctt catcctgctc tccgcttccg
     1621 tcgcgagcgc gctcgcggcg gcgctcgtga tgcgggccta cgcgcaccgg ctcgacctgg
     1681 cgcgcccgga cggcggtccc ccgatcgcgg tggtcgcggc cgtcgcacac gtcccgcgcg
     1741 gctcggtcct caccgagacc gtcctcgagg tcgtaacgct gccgtcccgc ttcgcgccgc
     1801 ccggcgcgat ccgcgacccg gctcgcgcca cgggacggat cgtggtcgcg gacctcgcgg
     1861 cgggcgagat cgtcacccgg ctccgactgg ccggttcgcg ctcgggtccg accgcctccc
     1921 tcgtcgaggc cggcatgcgg gcggtcgaga taccggtcgc gtcggcggtc ggtgtgcgac
     1981 ccggtgacct ggtcgacgtg atcgccacct tcgggggagg cggcgcccac acggaggtgg
     2041 cgggcgaagc gatcgaggtg ctcgccgtcg accgggcggg cggtggcgcc tccttcggag
     2101 gc
//
LOCUS       DASUVE010000167         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_273261, whole genome shotgun sequence.
ACCESSION   DASUVE010000167 DASUVE010000000
VERSION     DASUVE010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273261"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VF036_03570"
     CDS             <1..505
                     /locus_tag="VF036_03570"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX6264341.1"
                     /translation="DYPLHLGVTEAGPMPMGGVKSAVGIGTLLAEGIGDTIRVSLTDD
                     PVEEVKVGTGILQSLGLRKRGLDLVACPSCGRAEVDVLGLTKQVHEALEREGLKVPIR
                     VAVMGCVVNGPGEAREADLGIAAGKGQGFLVVKGEVVDKIPETDFVTRLVAEAKKIAA
                     TTPVDSG"
     gene            547..1305
                     /locus_tag="VF036_03575"
     CDS             547..1305
                     /locus_tag="VF036_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264342.1"
                     /translation="MKRILGRSIAAAALVLSAVPSMPAGAADVPGTTCSVFPPDNAWN
                     MDIRKLPRHPKSGTWKRATDAGSTWLHPDFGPPSYGIPFDVVPPSHPKVSVDFHYDDE
                     SDPGPYPFGGDVQIEGGSDRHAIMIDEGTCLLYELFGVHRNGGHPTAGSGAIFDLASN
                     ALRPAGWTSADAAGLPIFPGLVRYDEVFGPDPGIHHALRFTVSCTRRAYVWPARHQAG
                     VADRDCPPMERGSCSRVATTSPATPRRRRSCCER"
     gene            1302..1505
                     /locus_tag="VF036_03580"
     CDS             1302..1505
                     /locus_tag="VF036_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264343.1"
                     /translation="MKRYGMIVADNGSDWYFQGAVDPRWTFGFVDQLKRVPAGAFVAV
                     DVSDCKAAPNSAAFAYGPGCPAP"
     gene            complement(1506..2072)
                     /locus_tag="VF036_03585"
     CDS             complement(1506..2072)
                     /locus_tag="VF036_03585"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX6264344.1"
                     /translation="MILVVDHYDSFTYNLVQLIEGLGHPTEIVKSDEVPAADLVAREP
                     EAVVLSPGPGHPRDAGSFPDLLRLLPERIPVLGVCLGHQALGIASGGTVDRTTPFHGT
                     ASLVHHKGQGILDGVPSPFEAGRYHSLVVLRDDLPADLELTAWTEDGLVMATEHRQLP
                     RFGVQFHPESILTPEGPRIVENFLSLAS"
BASE COUNT          335 a          749 c          768 g          346 t
ORIGIN      
        1 cgactacccg ctgcacctcg gcgtcaccga ggccggcccg atgccgatgg gcggtgtcaa
       61 gagcgccgtc gggatcggga cgctcctcgc cgaggggatc ggcgacacga tccgcgtctc
      121 gctgaccgac gatccggtgg aggaggtcaa ggtcggcacg gggatcctgc agtcgcttgg
      181 cctgcggaag cgcggcctcg atctcgtcgc gtgcccctcc tgcggacggg cggaggtgga
      241 tgtgctgggg ctcacgaagc aggtgcacga ggccttggag cgcgaggggc tgaaggtgcc
      301 gatccgcgtc gcggtgatgg ggtgcgtcgt gaacggcccc ggcgaggcgc gcgaggcgga
      361 cctcgggatc gccgcgggca aaggccaagg gttcctcgtc gtcaaaggcg aggtcgtcga
      421 caagatcccc gagaccgact tcgtcacgcg cttggtggcg gaggcgaaga agatcgccgc
      481 caccacaccc gtcgactccg gctgagctcg gctcaacgcg acgtcgcagc gaccgatacc
      541 cgtgagatga agcgcatcct cggacgatcg atcgccgccg cggcgctcgt cctctcggcg
      601 gtgccgtcga tgccggccgg cgcggccgac gtcccgggca ccacgtgctc ggtcttccct
      661 ccggacaacg cgtggaacat ggacatccgg aagctgcccc gccatcccaa gagcggcacc
      721 tggaagcgtg cgacggacgc cggctcgacc tggctgcatc ccgacttcgg tcctccctcg
      781 tacgggatcc ccttcgacgt cgtgccgccg tcgcatccga aggtctcggt cgacttccac
      841 tacgacgacg agagcgatcc gggcccctat ccgttcggcg gcgacgtgca gatcgagggt
      901 ggctccgacc gccacgcgat catgatcgac gagggcacgt gcctgctcta cgagctgttc
      961 ggcgttcacc gcaacggcgg gcatcccacc gcgggcagcg gtgcgatctt cgacctcgcg
     1021 tcgaacgcgc tccggccggc gggatggacg agcgccgacg ccgccggcct cccgatcttc
     1081 cccggcctgg tccgctacga cgaggtgttc ggacctgacc ccgggatcca tcacgcgctg
     1141 cggttcaccg tgtcctgcac ccgtcgcgcc tacgtctggc ccgctcggca ccaggccggt
     1201 gtcgcggacc gggactgccc gccgatggag cgcggttcct gctcaagggt ggctacgaca
     1261 tcaccggcta ctcctcgaag gcgcagatcg tgctgcgagc gatgaagcgc tacgggatga
     1321 tcgtcgccga caacgggagc gactggtact tccagggagc cgtcgatccg cggtggacgt
     1381 tcgggttcgt cgatcagctc aagcgcgtgc ccgccggcgc cttcgtcgct gtggacgtgt
     1441 cggactgcaa ggcggctccc aactccgcgg cgttcgccta cggccccgga tgcccggctc
     1501 cgtagtcagc tcgccagact caggaagttc tccacgatcc tcggtccctc cggcgtgagg
     1561 atcgactcgg ggtggaactg gacgccgaac cggggcagtt ggcgatgctc ggttgccatc
     1621 acgagcccgt cctcggtcca ggccgtgagc tcgaggtcgg cgggcaggtc gtcccggagc
     1681 acgacgaggg agtggtaccg gcccgcttcg aacgggctcg ggacaccgtc gaggatcccc
     1741 tgtcccttgt gatggacgag ggacgccgtc ccgtggaacg gcgtggtgcg atccacggtc
     1801 ccaccgctcg cgatgcccag cgcctggtgc ccgaggcaca cgccgaggac ggggatccgc
     1861 tcaggaagca gtcggaggag atccggaaag gagcccgcgt cgcgtgggtg accggggccg
     1921 ggggagagca cgacggcttc cggttcgcgg gccacgaggt cggcggccgg cacttcgtcc
     1981 gacttcacga tctcggtcgg atgcccgagt ccctcgatca actggacgag gttgtaggtg
     2041 aagctgtcgt agtgatcgac gacgaggatc atgacgagat cggcacctct tcgtccgatg
     2101 gcttccgggc gatcacccag tggaacgtcg ccggtccctc tccttcctcg agctcggtca
     2161 cggtgtcggc gaactcgata gagaacccga cctcctcg
//
LOCUS       DASUVE010000168         2698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_650051, whole genome shotgun sequence.
ACCESSION   DASUVE010000168 DASUVE010000000
VERSION     DASUVE010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650051"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /gene="pilO"
                     /locus_tag="VF036_03590"
     CDS             <1..628
                     /gene="pilO"
                     /locus_tag="VF036_03590"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX6264345.1"
                     /translation="WSRSAGSRQVGRPMQGRRAIIVVGAGVAILALILIFFLVLPKMG
                     QVTEAEKKLEETTAQQQVLQARLNALEQARDEAPLNEATILRIDQQVPPTADLSGVIL
                     LLRNAASVAGVQVISLTPAPPTTATSGGFSTISVSASGECSYFAMVKYLHEIETLPRA
                     ATVEAVNLSPTEGSTLSFTATITFYTSDLSAGPGSEPGPTETVAVPGG"
     gene            632..1210
                     /locus_tag="VF036_03595"
     CDS             632..1210
                     /locus_tag="VF036_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264346.1"
                     /translation="MALSERDRRTVTIGGVVVGVLLAGFVLFSVLGGGEEAFPPIPDT
                     PLPTSSPSPPPDGGPPIQSFTGRDPFSVPPGLSPSPSVTVPGPSGGPSGGPSGSPSGS
                     PSVGPSGTPSPTAPGNDSSQTVGGSTVVLLDIFERDGATRAQVEVDGTVFDVGIGDQF
                     SGGRFELRSVAGNCATFLFGDESFTLCITPQK"
     gene            1328..2482
                     /gene="aroC"
                     /locus_tag="VF036_03600"
     CDS             1328..2482
                     /gene="aroC"
                     /locus_tag="VF036_03600"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX6264347.1"
                     /translation="MLRLLTAGESHGKALVAVLEGMPAAVPVAPKQIAAELARRRHGH
                     GRGGRQKLETDAPEILSGIRHGTTLGSPIAITIPNAEWERKYRDLMSVEGEGDPADRL
                     TRPRPGHADLAGAMKYGFDDVRNVLERASARETAARVAIGAVCKAFLAELDIRIVSHV
                     VRIGKVAVRAASRWPAPEDLEAVDASPVRCFDGTATEKMVAEIDRIRKDRDTVGGEFE
                     VIAYGCPPGLGSHVHYDRKLDARLALGLMSIQSVKGVGVGDGFASAARYGSAAHDEIV
                     RRDGKIARATARAGGIEGGMSTGQPIRLRAAMKPFSSVPKPLATIDLATGEDAVAIKQ
                     RTDVCAVPAGGVVGEAVVAFELANAVLEKFGGDSLPEIRRNLDGFVAGLP"
     gene            2500..>2698
                     /locus_tag="VF036_03605"
     CDS             2500..>2698
                     /locus_tag="VF036_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008382765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX6264348.1"
                     /translation="MPGAGKSTVGRELAGQLGVPFVDLDGEIEREAGAAVTDIFRDEG
                     EAAFRALEAAALVKASMQDPSV"
BASE COUNT          393 a          900 c          996 g          409 t
ORIGIN      
        1 gtggtcacgg agcgcgggca gcaggcaggt gggacggccg atgcagggta ggcgcgcgat
       61 catcgtcgtt ggggctgggg tggcgatcct cgccctgatc ctcatcttct tcctcgtcct
      121 cccgaagatg ggccaggtca cggaggcgga gaaaaagctc gaggaaacga ccgcgcagca
      181 gcaggtgttg caggctcggt tgaacgccct cgagcaggcc cgcgacgaag cgcccttgaa
      241 cgaggcgacg atccttcgga tcgatcagca ggtgccgccc acggccgatc tttcgggggt
      301 gatccttctc ctccggaacg ccgcctcggt ggccggtgtg caggtgatca gcctcacgcc
      361 ggcgccaccc acgacggcga cgagcggcgg attctcgacc atctcggttt cggcgagcgg
      421 cgagtgttcg tacttcgcga tggtgaagta cctgcacgag atcgagacgc tgcctcgcgc
      481 ggcgaccgtc gaagcggtca acctgtcgcc gaccgagggg tccacgctct cgttcacggc
      541 gacgatcacg ttctacacga gcgacctcag cgccggaccc ggttcggagc ccggtccgac
      601 agagaccgtc gcggtcccgg gaggatgaac gatggcgcta tctgaacggg atcgtcggac
      661 cgtcacgatc ggtggcgtcg tggtcggcgt actcctcgcg gggttcgtcc tcttctcggt
      721 cctcggtggc ggcgaggagg cgttcccgcc gatcccggac accccgctgc ccacgagctc
      781 cccgagtccg cctcccgacg gaggtcctcc gatccagagc ttcaccgggc gggatccctt
      841 ctccgtcccg cccggtctgt cgccctcgcc gtccgtcacg gttccggggc cgtcgggtgg
      901 cccgtcgggg ggcccgagcg ggagcccgag cgggagccca tccgtcgggc cgtcggggac
      961 gccgtccccc acggcaccgg gcaacgactc gagtcagacc gtcggagggt cgaccgttgt
     1021 cctcctcgac atcttcgagc gggacggcgc gacccgagcc caggtcgagg tggacgggac
     1081 cgtcttcgac gtcggaatcg gggatcagtt ctcggggggc aggttcgagc tgcgatcggt
     1141 cgcagggaac tgtgccacgt tcctgttcgg cgacgagtcg ttcacgctct gcatcacgcc
     1201 ccagaaatag ctccggcgac ccgcccacgg ggggagcggt cgccggcgcg ggggcggcgc
     1261 tcgcgccgcc cccgcatttc ctttttcccg tttctgggcc ggccataggg tccgatacga
     1321 tgctcccgtg ctcaggctgc tcaccgccgg tgagtcccac ggcaaggcgc tcgtcgccgt
     1381 cctcgaaggg atgccggcgg ccgtgccggt ggccccgaag cagatcgcgg cagagctcgc
     1441 gcggcgccgg cacgggcatg gtcgtggcgg ccggcagaag ctcgagaccg atgcgcccga
     1501 gatcttgagc ggcatccgcc acggcaccac gctcggctca ccgatcgcga tcacgatccc
     1561 gaacgcggag tgggagcgga agtatcggga cctgatgtcc gtggagggcg agggggatcc
     1621 ggcggatcgg ctgacccgac cccgcccggg acacgccgac ctcgcgggtg cgatgaagta
     1681 cggcttcgac gacgtgcgca acgtcctgga gcgggcgagc gcccgcgaga cggcagcgcg
     1741 cgtggcgatc ggggccgtct gcaaggcctt cctggccgag ctcgacatcc ggatcgtgtc
     1801 ccatgtcgtt cggatcggga aggtcgcggt gcgtgcggcg agccgctggc ccgccccgga
     1861 ggacctcgag gcggtggacg catcgccggt ccggtgcttc gacggaaccg cgaccgagaa
     1921 gatggtggcc gagatcgatc ggatccggaa ggatcgggac acggtcgggg gcgagttcga
     1981 ggtgatcgcc tacggatgcc cgcccgggct cggctcgcat gtccactacg accggaagct
     2041 cgacgcccgt ctcgctctcg ggttgatgag catccagtcg gtgaagggcg tcggggtcgg
     2101 agacgggttc gcctccgccg cacgctacgg ctcggcggcc cacgacgaga tcgtccggcg
     2161 cgatgggaag atcgccaggg cgaccgctcg tgcaggcggg atcgaagggg ggatgagcac
     2221 cgggcagccg atccggctac gagcggcgat gaagcccttc tccagcgtgc cgaagcccct
     2281 ggcgacgatc gatctcgcga cgggcgagga cgccgtcgcg atcaagcagc ggaccgacgt
     2341 ctgcgccgtt cccgcgggtg gcgtggtcgg cgaggccgtg gtcgcgttcg agctcgccaa
     2401 cgccgtcctg gagaagttcg gcggcgactc gctgcccgag atccgccgca acctcgacgg
     2461 cttcgtggcg gggctgccgt gatcgtctac ctggtgggca tgcccggagc cgggaagtcc
     2521 acggtcggca gggagctcgc cgggcagctc ggcgtgccgt tcgtcgacct cgatggcgag
     2581 atcgagcggg aagccggcgc ggcggtcacc gacatcttcc gggacgaggg cgaggcggcg
     2641 ttccgggcgc tggaggccgc ggcgctcgtg aaggcatcga tgcaggatcc gtccgtgg
//
LOCUS       DASUVE010000169         3162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1027514, whole genome shotgun sequence.
ACCESSION   DASUVE010000169 DASUVE010000000
VERSION     DASUVE010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027514"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VF036_03610"
     CDS             <1..532
                     /locus_tag="VF036_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6264349.1"
                     /translation="GFDFVLQQISYLPERAKSVDFSEMYYDVHQALVSVRGSPIADAT
                     TLEQLKDATLGAPIGTTSLSFIEDTIRPTTRPAVYDDLGAAVDDLKNGQVDGIVADLP
                     TASFITTAQVPEGVVVGRLPSVGSQEYLAMAFEKGSPIVECVNLALQEMRDDGSLDAI
                     RRTWLEGAADAPLIRD"
     gene            705..860
                     /locus_tag="VF036_03615"
     CDS             705..860
                     /locus_tag="VF036_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264350.1"
                     /translation="MLIRLFRGVIQDGAHDRLLRGLHDHVLPRLEAHPGVTSTTLALA
                     MEGSSDE"
     gene            864..1391
                     /locus_tag="VF036_03620"
     CDS             864..1391
                     /locus_tag="VF036_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX6264351.1"
                     /translation="MESHWRDVDDLIRFAGDDWRTPCVEPAEEDLLVSVSAHHYVTEG
                     PVVPSTAKLMTTQGSMRLGDVEIDGAGLRLAWNGSAVHLPPREMAAMLALARDVGASV
                     ASAELARRIWPGSAMVTPYDVRRVVHRLRLLLRSSGTPIHIRNVHGVGYGLDLEWPDE
                     PVRDTSRTSPGPHRV"
     gene            1443..1781
                     /locus_tag="VF036_03625"
     CDS             1443..1781
                     /locus_tag="VF036_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264352.1"
                     /translation="MAGTHEDAALVVELAKLGAMNGLGEAVGKIFADDFDPDAAELSD
                     PSVRIVLGWNETVATLVKNDLLSRDLVYDWLWVAGTWDRVGPAALRAREAAGVAVLYE
                     NFEALAAGQR"
     gene            complement(1807..2340)
                     /locus_tag="VF036_03630"
     CDS             complement(1807..2340)
                     /locus_tag="VF036_03630"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6264353.1"
                     /translation="MSNTTSGLDTFYEGWANYQGLVLDALRDLAAEQLGLRTAPHQWA
                     VWQLAGHVAGSRAYWFHDVLGEGDPATRDLFRVASTTVPDLPLEDAGWEDDEDHPRSA
                     AEIVEAFDRTWTMIEDCLRRWTTDDLAVEFTRERRSGTETFTRQWVIWHLVEHDLHHG
                     GEISQILGSHSAPALDL"
     gene            complement(2433..2975)
                     /locus_tag="VF036_03635"
     CDS             complement(2433..2975)
                     /locus_tag="VF036_03635"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6264354.1"
                     /translation="MTALFADLVGFTAFSEGKSASDVIEMLNRYWEGVVPVVVEAEGG
                     VIERFAGDAVMAVFNALDDQPDHALRGARAALAMRAFTQATATEHPDWPRFRIGVNTG
                     TAVIGTVGATDQRSFAAIGDTTNVAARVQAIARPGQVLLADATYQRMRDRVRVEPLGP
                     TSLKGRTENIDVYELLEVVS"
BASE COUNT          505 a         1062 c         1092 g          503 t
ORIGIN      
        1 gggcttcgac ttcgtgctcc agcagatctc ctacctcccg gagcgcgcga agagcgtgga
       61 cttcagcgag atgtactacg acgttcacca ggcgctcgtg tcggtccggg gctcaccgat
      121 cgccgacgcc acgacgctcg agcagctgaa ggacgccaca ctcggcgcac cgatcggtac
      181 cacgagcctc agcttcatcg aggacacgat ccgaccgacc acgcggccgg ccgtctacga
      241 tgatctcggc gccgcagtgg acgacctgaa gaacggacag gtcgatggca tcgtggcgga
      301 cctgccgacc gcatccttca tcactaccgc tcaggttccg gagggcgtgg tcgtcggccg
      361 tctcccgagc gtcggctcgc aggagtacct cgcgatggcg ttcgagaagg gaagcccgat
      421 cgtggagtgc gtgaacctcg cgctccagga gatgagggac gatggctcgc tcgacgcgat
      481 ccgccggacg tggctcgagg gcgcggccga cgcgccgctg atccgggact gacctcgggc
      541 gatcgaccgg cgccctcctc agccccgctc caaggctgcc aaggcgggtc gcggtccgtc
      601 gtcggcccaa gcgttccgaa cggttcggct tcgacgggat ccgccgacca cccggggaac
      661 cggggcgggg aacacctgtc ggtgccttcc gatagcgtcc ctccgtgctg atcagactgt
      721 tccggggggt gatccaggac ggggcgcacg accgactcct ccgcgggctc cacgaccacg
      781 tccttccgcg actcgaggca catccggggg tgacgtccac cacgctcgcc ctcgcgatgg
      841 agggctcgtc ggacgagtag ctggtggagt cccattggcg cgatgtcgac gatctgatcc
      901 ggttcgccgg cgacgattgg aggactccct gcgtggagcc cgccgaggag gacctcctcg
      961 tctccgtctc cgctcatcac tacgtgacgg agggacccgt tgttccgtcg acggcgaagc
     1021 tcatgacgac gcagggatcg atgcgcctgg gcgacgtgga gatcgacggt gccggcctcc
     1081 ggctcgcctg gaacggatcg gccgtgcatc ttccgccacg ggagatggcc gcgatgctcg
     1141 cgctcgcacg agatgtgggc gcctcggtcg cctccgccga gctcgcgcgg cgtatctggc
     1201 ccgggagcgc gatggtcacc ccgtacgacg ttcgccgggt cgtccaccgg ctgcgcctac
     1261 tcctccggtc gagcggcacg cccatccaca tccgcaacgt gcatggtgtc ggatacgggc
     1321 tggacctcga gtggcccgac gagcccgtcc gggacacatc caggacatct cccggaccac
     1381 accgggtgta gaaaaccggt gagcgaccgc gtgggcggcc gcctggacgc gaggaggaag
     1441 cgatggccgg gacccatgaa gacgccgcgc tcgtggtcga gctcgccaag ttgggagcga
     1501 tgaacgggct gggagaggcg gtggggaaga tattcgccga tgacttcgat cccgacgcag
     1561 ctgagctcag tgacccgtcg gttcgcatcg tcctcggctg gaacgagacg gtggcgacgc
     1621 tggtgaagaa cgacctgctg agccgggacc tcgtctacga ctggctgtgg gtggcgggca
     1681 cctgggaccg ggtcggcccg gccgccctcc gggcccgcga ggcggcaggt gttgccgttc
     1741 tctacgagaa cttcgaggcg ctggcggcag ggcagcgatg aaccgaggga tagcgagacc
     1801 ggagcctcag agatcgagtg cgggggcgct gtggcttccc aggatctgtg agatctcacc
     1861 gccgtggtgg aggtcgtgct cgacgaggtg ccagatcacc cactgacggg tgaacgtttc
     1921 cgtgcccgag cgacgctcgc gggtgaactc gacggcgagg tcgtcagtcg tccatcgccg
     1981 gaggcagtcc tcgatcatgg tccaggtccg atcgaatgcc tcgacgatct cggccgcgct
     2041 gcgagggtga tcctcgtcgt cctcccaccc ggcgtcctcc aggggcaggt cgggaacggt
     2101 ggtgctcgcg acgcggaaca ggtcgcgcgt ggcgggatca ccctcgccga ggacgtcgtg
     2161 gaaccagtaa gcgcgggatc ccgccacgtg accggcgagc tgccagacgg cccattggtg
     2221 tggagcggtc cgcagcccca actgctcggc ggcgagatca cggagcgcgt cgaggacgag
     2281 cccctggtag ttcgcccaac cctcgtagaa ggtgtccagt ccggacgtcg tgttcgacat
     2341 gcggcgcatg gtaggcgccc cgctccctca ggtcacctgt gggcaggaat gcaattcagc
     2401 cgtcgggatc gggccggcct ccttcgtcct ggtcaggaaa cgacctcgag cagttcgtag
     2461 acgtcgatgt tctccgtcct gcccttcaac gaggtgggac cgagcggttc gacgcgcacc
     2521 cgatcgcgca tccgctggta cgtggcatcc gccaggagca cctgcccagg gcgggcgatc
     2581 gcctggactc gcgccgcaac gttcgtcgtg tcaccgatgg cggcgaagct ccgttgatcg
     2641 gtcgcgccga cggtcccgat cacggccgtg ccggtgttca cgccgatccg gaaccgaggc
     2701 caatcgggat gctccgtcgc ggtcgcctgc gtgaacgctc gcatcgcgag cgcggctctc
     2761 gctccccgga gggcgtgatc aggctgatcg tcgagcgcgt tgaacacggc catgacggcg
     2821 tcccccgcga accgctcgat cacgccgcct tcagcctcga cgaccaccgg gacgacaccc
     2881 tcccagtatc gattgagcat ctcgatcacg tccgatgcgg acttcccttc actgaatgcg
     2941 gtgaatccga cgagatccgc gaacagcgcg gtcacgtccg cttcccggcc tccgagatcg
     3001 ccgaactcgg gttcttcgtg taagcgtgcc gcgagctacg gcccgacgta ggagccgaag
     3061 aggttgtcga cgcggacgag ctcgtgggcg gcgcgctcga gagaggcagc ctcctcggcg
     3121 ctcatgccct cggcgaggaa gccgtccacg acgggcgcga gc
//
LOCUS       DASUVE010000170         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1028457, whole genome shotgun sequence.
ACCESSION   DASUVE010000170 DASUVE010000000
VERSION     DASUVE010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028457"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VF036_03640"
     CDS             <1..481
                     /locus_tag="VF036_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX6264355.1"
                     /translation="TVIPWDGPPFSRTAEAEQRLRTCASTSDDWSTHAPAAGWEWWHH
                     EPQPREGPMSDRNRTEKLLERERTRLNDIRSSLTEVAEDVERDPTGDPSAGGHVADAG
                     SEMFERSRDLSIISEIDAQLIDIDHAEARLSNGTYGTCEACGTAIGPERRAVRPATR"
     gene            610..810
                     /locus_tag="VF036_03645"
     CDS             610..810
                     /locus_tag="VF036_03645"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX6264356.1"
                     /translation="MRGPIRLVDERGDEVPVRRRVNAICRCGRSATQPFCDGTHKIVP
                     FSPRAVPVASAGGPEGESPLAG"
     gene            complement(743..1720)
                     /locus_tag="VF036_03650"
     CDS             complement(743..1720)
                     /locus_tag="VF036_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6264357.1"
                     /translation="MSQDLSYILPYRSRPGDGNEEELDRYVRWLSRRVQLIVVDGSDP
                     DRVRAHREQWGSIATLAVPDADVRCLNGKVRGVLTGLRRARHEHVIIADDDVRYDDEA
                     LLRMQELLRNADLVRPQNFFDPIPWHARWDTSRMLLNRALGNDHPGTLGVRRSFLTSI
                     GGYDGDVLFENLELVRTVLAAGGRVVDAPEMYVRRLPPTTARFLEQRPRQAYDDLAQP
                     ARLAFFLVLGPALWTVARRRPGGLLAAAVASVLVAQVGRLRHGGNRVFDRVSPFFAPF
                     WMVERACLVWLAVFRRFSRGGCTYAGGVIRRAATPPRVLRRRLRGRLAG"
     gene            complement(1717..>2576)
                     /locus_tag="VF036_03655"
     CDS             complement(1717..>2576)
                     /locus_tag="VF036_03655"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6264358.1"
                     /translation="LGLAYELSYRGLPGVPDDLEWDPEVLGARRNLEEAFEAALRHTI
                     GREAVDPRDVPGRLGEIATPPDADSLSAFIEREATLDQVREFVMHRSAYQLKEADPHS
                     WVIPRLDGAAKTAVMEIQYDEYGSGDPAWMHSELFRTTMIAAGLDGSYGAYVDRLPGT
                     TLATVNLITLFGVHRRWRGALVGHLAAFEMTSSLPNRAYAKGFRRLGFDDDAVRFFDE
                     HVEADSVHEVLAGDLAAGLAVEEPELASDVVFGAMAMALLEGRFADGLLRAWRAGDST
                     LLPAGVRSW"
BASE COUNT          437 a          898 c          893 g          348 t
ORIGIN      
        1 aacggtcatc ccttgggatg gaccgccctt cagccgaacg gccgaggcgg aacagcggct
       61 gcggacctgt gcttcgacga gcgatgactg gtcgacgcat gcaccggccg ccggatggga
      121 atggtggcac cacgagcccc aaccacggga gggacccatg tccgaccgga accgcaccga
      181 gaagttgctc gaacgggagc gcacccgcct caacgacatc cggtcaagcc tgacggaggt
      241 cgcggaggac gtggagcgcg atcccaccgg cgacccctcc gccggcggcc acgtggccga
      301 cgccggatcg gagatgttcg aacgttcccg ggatctctcg atcatcagcg agatcgacgc
      361 acagctgatc gacatcgacc acgcagaggc ccgcctctcg aacggcacct acgggacgtg
      421 cgaggcgtgc gggacggcga tcgggcccga gaggcgggcc gtgcgcccgg ccacccggta
      481 gtgcatcgag gatcagatcg gcgtggagcg cgagatgcag atccagggca tgtcgagtcg
      541 cgagcgcagc tcgtgagccg ccgatccgag gtgggcgtcg tccgcgtcac cgcggacggt
      601 ccgctgctcg tgcgagggcc gatccgcctc gtcgacgagc gcggagacga ggtcccggtc
      661 cggcgccgcg tgaacgcgat ctgtcgatgc ggccgctcgg ccacgcagcc gttctgcgac
      721 ggcacccaca agatcgtgcc gttcagcccg cgagccgtcc ccgtagcctc cgccggagga
      781 cccgaggggg agtcgccgct cgccggatga cgccgcccgc atacgtgcaa cctccccgcg
      841 agaacctccg gaagaccgcc agccagacca ggcacgcgcg ctccaccatc cagaaggggg
      901 cgaagaacgg agagactcgg tcgaagaccc tgttgccgcc gtgccggagc cggcccacct
      961 gcgccacgag gaccgatgcg acggccgccg cgaggagacc cccaggtcga cgccgcgcca
     1021 ccgtccaaag cgccggcccg agcaccagga agaacgccag ccgggcgggt tgcgccaggt
     1081 cgtcgtacgc ctggcgagga cgttgttcca ggaatcgagc ggtggtgggc ggcaatctgc
     1141 gcacgtacat ctcgggcgcg tcgacgaccc ttcctcccgc cgcgagcacc gtcctgacca
     1201 gctcgaggtt ctcgaagagc acgtcgccgt cgtagccacc gatggacgtg aggaaggagc
     1261 gccgcacgcc cagggtcccg gggtggtcgt tcccgagcgc ccggttgagc agcatccgcg
     1321 acgtgtccca gcgggcgtgc cacgggatcg ggtcgaagaa gttctgtggc cggacgaggt
     1381 cggcgttccg aaggagctcc tgcatccgga gcagagcctc gtcgtcgtag cgaacgtcgt
     1441 cgtcggcgat gatcacgtgc tcgtgccggg ctcggcgtag cccggtgagg acgcctcgga
     1501 ccttgccgtt caggcatctg acatccgcat cgggcacggc aagggtggcg atcgagcccc
     1561 actgctcccg gtgcgcacgg acacggtcgg ggtccgagcc gtcgacgacg atcagctgaa
     1621 ccctccggga cagccatcgg acgtagcgat cgagctcctc ttcgttcccg tccccgggac
     1681 ggctccggta ggggaggatg tacgagagat cctggctcac cacgaccgca cgccagccgg
     1741 gaggagcgtc gagtcgccgg cccgccacgc tcggagcagt ccgtctgcga agcgaccttc
     1801 gagcagcgcc atcgccatcg ccccgaagac gacgtcgctc gcgagctcgg gctcctccac
     1861 agcgagaccg gcggcgagat cgccggcgag gacctcgtgg acggagtcgg cctcgacgtg
     1921 ctcgtcgaag aaccgcaccg cgtcatcgtc gaacccgagc cgtcggaacc ctttcgcgta
     1981 ggcgcggttc ggcaggctcg acgtcatctc gaacgccgcg aggtggccga cgagggctcc
     2041 tcgccatcgg cggtgtaccc cgaacagcgt gatcaggttc acggtcgcga gggtcgtccc
     2101 cgggagccgg tcgacgtagg ccccgtagga gccatcgaga cccgccgcga tcatcgtggt
     2161 gcggaagagc tcggaatgca tccatgcggg atccccgctg ccgtactcgt cgtactggat
     2221 ctccatgacg gccgtcttcg cggctccgtc cagacgcggg atcacccagg agtgcggatc
     2281 cgcctccttg agctggtagg ccgagcgatg catcacgaac tcccgtacct ggtcgagcgt
     2341 cgcctcccgc tcgatgaacg ccgagagcga gtcggcatcc ggtggcgtgg cgatctcccc
     2401 gagccggccg gggacgtcgc gcggatccac ggcctccctg ccgatcgtgt gacgcaacgc
     2461 ggcctcgaac gcctcctcga ggttgcgacg ggcgccgagg acctcaggat cccattcgag
     2521 gtcatcaggc acgccgggca agcctcgata ggaaagctcg taggcgaggc cgagcg
//
LOCUS       DASUVE010000171         5113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_86935, whole genome shotgun sequence.
ACCESSION   DASUVE010000171 DASUVE010000000
VERSION     DASUVE010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86935"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VF036_03660"
     CDS             complement(<1..248)
                     /locus_tag="VF036_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002833327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX6264359.1"
                     /translation="MQVSQTEQKVKDGKGLAIREYPLARTRNIGIMAHIDAGKTTTTE
                     RILYYTGKAYKIGEVHEGTAQMDWMVQERERGITITSAA"
     gene            complement(331..801)
                     /gene="rpsG"
                     /locus_tag="VF036_03665"
     CDS             complement(331..801)
                     /gene="rpsG"
                     /locus_tag="VF036_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX6264360.1"
                     /translation="MPRKGPAVHRGITPDPVYQNPLVTQLVNKVLLHGKKTVAEHIVY
                     EALEQISQKTANDPVITLKRAVDNVRPMLEVKSRRVGGASYQVPVEVKPTRGTTLALR
                     WLVNFSRARRENSMAERLVAEIMDASNGQGASVKRREDMHKMAEANRAFAHYRW"
     gene            complement(803..1177)
                     /gene="rpsL"
                     /locus_tag="VF036_03670"
     CDS             complement(803..1177)
                     /gene="rpsL"
                     /locus_tag="VF036_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX6264361.1"
                     /translation="MPTIQQLVRKGRSRPKKRTKSPALKGSSQRRGVCTRVYTMTPKK
                     PNSALRKVARVRLTSGIEVTVYIPGVGHNLQEHSIVLVRGGRVRDLPGVRYKIIRGTL
                     DTAGVRDRRKARSRYGAKKVGE"
     gene            complement(1491..>5113)
                     /locus_tag="VF036_03675"
     CDS             complement(1491..>5113)
                     /locus_tag="VF036_03675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX6264362.1"
                     /translation="VTHIWYFKGVPSRLGYLLDIAPKDLERIIYFAAYVVTRIDDERR
                     HKDLPEIEEEIEGEKREIEAERDDQVKRRLSELEDRLKELEAEGAKGAQLSAAKREAQ
                     RDVERIHEAAARDTQHLDDVVSAFKSLKVKQLVADDAVYRSLRERFADYFDGGMGAEA
                     IKRLLQDYDVVTEGEALRETIENGKGTKRLKAIKRLKVASNFVEGRSSPMGMVLDAIP
                     VIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLQAPEIIVNNEKRMLQE
                     AVDALFDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGP
                     ELRLHQCGLPKQMALELFKPFVMKRLVDQNLAQNIKSAKRMVERARPQVWDVLEEVIR
                     EHPVMLNRAPTLHRLGIQAFEPVLVEGKAIKIHPLVCAAFNADFDGDQMAVHVPLSAE
                     AQAEARILMLSTNNLLSPAHGRPIATPSQDMVLGLYYLTLTQNEDLKDEEIPTFGSTA
                     EALLALDRGHPLHDPVRVRLTGQALPEELLPEGYDAKADGWKGRTPVIRTTIGRIVFN
                     TAFPDDFEFRNEPVLKPDVARIVDEVVHRYDRAQVEIVLDNLKNLGFDYATKAGVTIG
                     VEDVTTPPEKQRILDEHEKRAQKVEQQYRKGIITDDERRQELIEIWTQATDEVKDAME
                     AQFTKTNPIFMMANSGARGNIMQIRQLAGMRGLVANPKGEIIPRPIKSNFREGLSVLE
                     YFISTHGARKGLADTALRTADSGYLTRRLVDVAQEVIVREEDCGTDRSIGAVVNVTRP
                     DGTFVKGAHTDTALFGRVLAEDVKVGGKKIAARGAELSDELAQAVIDSGVETVQVRSV
                     TTCEAEIGVCQACYGRSLASGRLVDIGEAVGIIAAQSIGEPGTQLTMRTFHTGGIAGE
                     DITHGLPRVVELFEARNPKGMAQITELSGEVAIEDDDEKHVRRIRVTDETGDMVEYQV
                     SFRARLTVSNGDMVEVGQQLTEGSVNPHEKLRVEGVLALQMHLVDEVQQVYRSQGVTI
                     HDKHIELIARQMLRKVHIIEPGDSDLLPGDLVNRRVFEETNQEVVEAGGVPASARPVL
                     LGITKASLATDSWLSAAPFQETTRVLTDAAIQSKADPLLGLKENVIIGKLIPAGTGMS
                     RYRNVAVKIKPDAIPEYWLTRQRELAELAELAEGGEPALVGLTRQQAESMLGGVARSD
                     EE"
BASE COUNT          714 a         1753 c         1695 g          951 t
ORIGIN      
        1 gccgccgacg tgatcgtgat cccgcgctcg cgctcctgga ccatccagtc catctgcgcg
       61 gtgccttcgt gcacctcgcc gatcttgtag gccttcccgg tgtagtagag gatccgctcg
      121 gtcgtggtgg tcttcccggc gtcgatgtgc gccatgatgc cgatgttgcg cgtgcgagcg
      181 agggggtact cgcggatcgc gagccccttg ccgtccttca ccttctgttc ggtctgcgac
      241 acctgcatcc tctctgcggg gaccgccatc aggaggcccc ctccacacgg gaaaccggcc
      301 ccgtggggcc ggtcgggtgg tgctcgtcga tcaccatcgg taatgcgcga acgctcggtt
      361 ggcctcggcc atcttgtgca tgtcctcgcg ccgcttgacg gaggctccct gcccgttgga
      421 ggcatccatg atctccgcca cgaggcgttc ggccatcgag ttctcgcgcc gcgcgcggga
      481 gaagttcacg agccaccgca gcgcgagcgt cgtgccgcgg gtgggcttca cctccacggg
      541 gacctgatac gaggcgccgc cgacccgtcg cgacttgacc tccagcatcg ggcgaacgtt
      601 gtcaaccgca cgcttcaggg tgatcacggg gtcgttcgcg gtcttctgac tgatctgctc
      661 gagcgcctcg tagacgatgt gctcggcgac ggtcttcttg ccgtgcaaca gcaccttgtt
      721 gaccagctgg gtcacgaggg ggttctggta cacgggatcg ggcgtgatgc cccggtgtac
      781 ggcaggtccc ttgcgcggca cgttactcgc cgaccttctt ggcgccgtag cgcgaacggg
      841 ccttgcgtcg gtcgcggacc cctgcggtgt cgagggtgcc gcggatgatc ttgtagcgca
      901 cgccgggcag atcgcggacg cgaccgccgc ggacgagcac gatcgagtgc tcctggaggt
      961 tgtgtcccac gccggggatg tagacagtga cctcgatccc ggaggtgagc cggacccgcg
     1021 cgaccttccg cagggcggag ttcggcttct tgggagtcat cgtgtacaca cgcgtgcaga
     1081 cgcctcgccg ctgggacgag cccttcagcg cagggctctt cgtccgcttc ttcgggcggg
     1141 atcggccctt ccgtacgagt tgttggatgg taggcatggc tctctcgtct gcactcttcc
     1201 tttttcatcc accggggctt ggaagcctcg ggccgaaccc ttggggcgca gcgcgaacgc
     1261 caccacccgc ggggttgtcg ctccgaccgg agccggacgc ccgacctgct cgccgacccg
     1321 ggcgcgaccg ccaaagctac tggtcgtgag cacgacgtgt caaccgcacc ggatgccacc
     1381 cggcgtgagg gccggccaga gcggtttgca gccgtcatcg tacctgccga gcccgcgcgc
     1441 cgacacggcc gaccgggctc ccgggagatt cggggacccc gttcccccac ctactcctcg
     1501 tcggatcgag cgaccccgcc cagcatgctc tcggcctgct ggcgggtgag ccccaccaga
     1561 gccggttctc ccccttcggc cagctcggcc agctccgcca gctcccgctg acgcgtgagc
     1621 cagtactcgg ggatcgcatc gggcttgatc ttcacggcca cgttgcggta gcggctcatc
     1681 cccgtgcccg ccgggatcaa cttcccgatg atcacgttct ccttgaggcc cagcaacgga
     1741 tccgccttgc tctggatcgc cgcatccgtg agcacccggg tcgtctcctg gaacggggcg
     1801 gccgagagcc acgaatccgt cgcgagcgag gctttcgtga tgccgagcag gacgggccgg
     1861 gccgatgcag ggaccccgcc ggcctcgacc acttcctggt tggtctcctc gaacacgcgc
     1921 cggttcacga gatcgcccgg caggagatcg gagtccccgg gctcgatgat gtgcaccttc
     1981 cggagcatct gccgcgcgat cagctcgatg tgcttgtcgt ggatcgtcac gccctgagag
     2041 cggtagacct gctgcacctc gtccacgagg tgcatctgca gggccagcac gccttccacg
     2101 cggagcttct cgtgcgggtt caccgaaccc tcagtgagct gctgacccac ctccaccatg
     2161 tcgccgttcg acaccgtgag ccgagcgcgg aacgagacct ggtactcgac catgtcgccg
     2221 gtctcgtccg tgacccggat gcgccggacg tgcttctcgt cgtcgtcctc gatcgccacc
     2281 tcgcccgaga gctcggtgat ctgcgccatc cccttggggt tgcgcgcctc gaacagctcg
     2341 accacgcggg gcaggccgtg cgtgatgtcc tcgccggcga tcccgccggt gtggaaggtt
     2401 cgcatcgtga gctgggtgcc gggctcgccg atcgactggg cggcgatgat gccgaccgcc
     2461 tcgccgatgt cgacgaggcg cccgctcgcg agcgagcggc cgtagcacgc ctgacacacg
     2521 ccgatctccg cctcacaggt ggtgaccgag cggacctgga cagtctccac gccggagtcg
     2581 atgaccgctt gggcgagctc gtcggagagc tcggctccac gggcggcgat cttcttgccg
     2641 ccgaccttca cgtcctccgc gaggacgcgc ccgaacaacg cggtgtcggt gtgggcgccc
     2701 ttcacgaagg tcccgtcggg acgcgtgacg ttcaccaccg caccgatcga tcgatcggtg
     2761 ccgcagtcct cctcgcggac gatgacctcc tgggcgacgt cgacgagtcg ccgtgtgagg
     2821 tagcccgagt cagcggtccg cagggcagtg tcagcgagac ccttccgggc gccgtgggtg
     2881 gagatgaagt attcgaggac cgacagaccc tcgcggaagt tggacttgat cgggcgcggg
     2941 atgatctcgc ccttcgggtt cgcgacgaga ccgcgcatcc cggccagctg ccggatctgc
     3001 atgatgttcc cgcgcgcgcc cgagttggcc atcatgaaga tcgggttcgt cttcgtgaac
     3061 tgcgcctcca tcgcgtcctt gacctcgtcg gtggcctgcg tccagatctc gatgagctcc
     3121 tggcgacgct cgtcgtccgt gatgatgccc ttccggtact gctgctcgac cttctgggca
     3181 cgcttctcgt gctcgtcgag gatcctctgc ttctccggcg gcgtcgtgac gtcttcgacg
     3241 ccgatcgtga cgcccgcctt cgtggcgtag tcgaacccga ggttcttcag gttgtcgagg
     3301 acgatctcga cctgcgcccg gtcgtagcgg tggaccacct cgtcgacgat ccgcgccaca
     3361 tccggcttca agaccggctc gttgcggaac tcgaagtcgt ccgggaacgc cgtgttgaag
     3421 acgatccggc cgatggtcgt ccggatgacc ggcgtgcggc ccttccatcc gtcggccttg
     3481 gcgtcgtagc cctccgggag gagctcctcg gggagcgcct ggcccgtcaa gcgcacgcgc
     3541 accggatcgt gcagcgggtg accgcggtcg agggccagca acgcctcggc ggtcgagccg
     3601 aacgtcggga tctcctcgtc cttcaggtcc tcgttctgcg tgagggtcag gtagtagagc
     3661 ccgagcacca tgtcctgcga cggagtggcg atcgggcggc cgtgcgccgg cgacaggagg
     3721 ttgttcgtcg agagcatcag gatccgggct tcggcctgcg cctcggcgga cagcgggacg
     3781 tgcacggcca tctggtctcc gtcgaagtcg gcgttgaacg ccgcgcagac gaggggatgg
     3841 atcttgatcg ccttgccctc gaccagcacg ggctcgaacg cctggatccc cagacggtgc
     3901 agcgtgggcg cccggttcag catcacgggg tgctcccgga tgacctcctc gagcacgtcc
     3961 cacacctgcg gacgggcacg ctccaccatg cgcttggcgc tcttgatgtt ctgggcgagg
     4021 ttctggtcga cgagccgctt catcacgaac ggcttgaaga gctcgagcgc catctgcttc
     4081 ggcagcccgc actggtgcag gcggagctcc ggcccgacca cgatgaccga gcggcccgag
     4141 tagtcgacgc gcttgcccag caggttctgg cggaagcggc cctgcttgcc cttcagcatg
     4201 tcggagagcg acttcagcgg ccggttcccc ggtcccgtga cgggacgacc ccgacggccg
     4261 ttgtcgaaca gggcgtcgac ggcctcctgg agcatccgct tctcgttgtt cacgatgatc
     4321 tccggtgcct gcaggtcgag cagccgcttc agccggttgt tccggttgat gacgcgccgg
     4381 tacaggtcgt tgagatccga ggtcgcgaac cgaccgccgt ccagttgcac catcggacgg
     4441 aggtccggtg ggatcaccgg gatggcgtcg agcaccatgc ccatcgggct cgagcgcccc
     4501 tcgacgaagt tggacgcgac cttcagacgc ttgatcgcct tcagtcgctt cgtgcccttg
     4561 ccgttctcga tcgtctcccg aagggcctct ccctcggtga cgacgtcgta gtcctggagc
     4621 agccgcttga tggcctcggc gcccatgcca ccgtcgaagt agtccgcgaa gcgctcgcgg
     4681 agcgagcggt agacggcgtc gtcggcgacg agctgcttga ccttgaggct cttgaacgca
     4741 gacaccacgt cgtcgaggtg ctgggtatcg cgggcggcgg cctcgtggat gcgctccacg
     4801 tctcgctgcg cctcccgctt ggcggcggag agctgtgcgc ccttggcacc ctcggcctcg
     4861 agctccttca gacggtcctc gagctccgag agcctgcgct tgacctggtc gtcccgctcg
     4921 gcttcgatct cgcgcttctc gccctcgatc tcctcttcga tctcggggag gtccttgtgg
     4981 cgccgctcgt cgtcgatccg ggtcaccacg taggcggcga agtagatgat ccgctcgagg
     5041 tccttggggg cgatgtcgag cagatagccc agacgggagg gaacgccctt gaagtaccag
     5101 atgtgcgtga cgg
//
LOCUS       DASUVE010000172         5148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_652253, whole genome shotgun sequence.
ACCESSION   DASUVE010000172 DASUVE010000000
VERSION     DASUVE010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652253"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1243)
                     /gene="alr"
                     /locus_tag="VF036_03680"
     CDS             complement(146..1243)
                     /gene="alr"
                     /locus_tag="VF036_03680"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX6264363.1"
                     /translation="MSEGYRPTWVEVDLEAIRHNARVLKPETAELMAVVKANGYGHGD
                     VEVARAAIEAGATWCGVALVEEGLRLRSAGIETPVLVLSELPAGSEAVALAHRLTPTL
                     YSDGGLERLASATRGSVPVHVKVDTGMHRVGVWPPEDAPAFARRVQEAGFEIEGLFTH
                     FARSEEDEATTKEQLDRFLDAAETARTARVRARVLHAANSGATILHPESHLDLVRPGI
                     ALYGIEPAPGIGAHLGLRPALTWRSRVSAVKRLTVGEAVSYGHRYRLERDAWLATVPV
                     GYADGYPRQLTNLGSVLIGGRRHGIAGTVTMDQLLVDCADEEVQVGEEVVLIGNQGDE
                     TIGAEELGQLIGTIGYEIVSRIGDRVPRRYR"
     gene            complement(1240..2772)
                     /locus_tag="VF036_03685"
     CDS             complement(1240..2772)
                     /locus_tag="VF036_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX6264364.1"
                     /translation="MKPVLTPEEAAALDRDTQSRGIAAEALMERAGRAVARATRELAG
                     GTYGRRAVVVCGKGNNGGDGLVATRHLARNGVRTTVVLLGDPDELRDPAASNARRLAA
                     VPGIRARTFDEHALRRELVRADIVVDAIFGTGFRGMPEDDWATAIASLNEADAPVVAV
                     DIPSGVDGATGAVEGEAVRADLTVALGAPKLGTVLMPGAEHAGILRVVDIGFPDDLLR
                     ARAWLTEPDDVAGWLPKRDIDTHKRASGVLVVVAGSRAMTGAARLIAMAGGRVGAGLV
                     VVAAPEGCLSQIQAGLTEPTFLPLPETADGTVAAEAAAPVLERLEAADALAIGPGLTT
                     DQATVAFVRDIVRRSPVPLVLDADGLNAFTADGAALGDRASEAVLTPHVGEFGRLTGV
                     KARDLDADRPTHVRALANQTGAVTLLKGSRTVIANSDGRLFVNVTGSPVLATAGTGDV
                     LTGMIGGLLARGVAPTESAAAAAYLHGLAGLFAGRDLGEGAVAGDVVERVPEAVAHVE
                     AE"
     gene            complement(2769..3140)
                     /gene="acpS"
                     /locus_tag="VF036_03690"
     CDS             complement(2769..3140)
                     /gene="acpS"
                     /locus_tag="VF036_03690"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX6264365.1"
                     /translation="MDIVGLGVDLCEIARMERAIARHPTFRDRVFTPEEIAYCDSKAR
                     PAESYAGRFSAREATIKALGGYRGRRWQDISVTRHPSGAPAIRLDGNAKRRADALGVT
                     QVLITFTHERTNAVAFAIAVR"
     gene            complement(3150..3725)
                     /locus_tag="VF036_03695"
     CDS             complement(3150..3725)
                     /locus_tag="VF036_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264366.1"
                     /translation="MTRAMHRPARLGHAIVLILTVAAGLLVASPARACSCAEMDLGSR
                     LPEADGAFIGTYVDRDPLSDGRSAWTFDVERVIKGPFGPTAIVRTSGDGASCGIELFD
                     DPRAGLLLDRANDGVWESSLCQQVPPNQLLSFAQNSHPPVSTIAPIGTAWSIESKVAL
                     VGALVVVIALGALVWFARRRPAATTGGSHVA"
     gene            complement(3735..>5148)
                     /gene="glmS"
                     /locus_tag="VF036_03700"
     CDS             complement(3735..>5148)
                     /gene="glmS"
                     /locus_tag="VF036_03700"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX6264367.1"
                     /translation="DCVRTACAPVVRELEGAYSLVVMSSEEPGLLVGVKVASPLVVGV
                     GAGESILASDIPAVLGRTTTVIPVDEGRIVEVRADGATFSDFDGAVVHPESIEVDWDT
                     ARAEKGGFDTFMLKEIHEQPSAIRDTLVGRVDESRRLALDDLKISDDVLREVNKVFVV
                     ACGTAYHSGLVAKYAIEHWTRLPVEIDIASEFRYRDPVLGPDTLTLAVSQSGETIDTL
                     EAARHARHQGSPLLAVTNTVGSSLAREADGVIYMHAGPEIGVAATKTFATQMVSQYIL
                     AMYLAQVRGSMFPEEIAEVLTRMAELPVQVERAIALDAQVQELARRFQDARDWLFIGR
                     HTGFPAALEGALKLKEISYVHAEGYPAGELKHGPIALVEDGVPVVAVATTCHVYPKML
                     SNIQEVRARGAEVIAVATEGDEQIRGVAQHVLEVPETPELLSPVVVTVPLQLLAYHVA
                     RLRGSDVDQPRNLAKSVTVE"
BASE COUNT          765 a         1904 c         1719 g          760 t
ORIGIN      
        1 tcctcggggg gcggacccca cgcgcggtcg ccgccggccg aacgccgttg tcgccagagc
       61 gctcgtcccg cgaggccggc ggcgaccgcc cccgcggcga cagccgcacc gacggccacc
      121 ctcggcgcgt tcgacatggc cttcctcatc ggtaccgcct cgggacgcgg tccccgatcc
      181 ggctgacgat ctcgtacccg atcgtcccga tgagttggcc gagctcctcc gccccgatcg
      241 tctcgtcccc ctggttcccg atcagcacga cctcctcgcc gacctggacc tcctcgtctg
      301 cgcagtccac gagcagctgg tccatcgtga ccgtcccggc gatcccgtgg cgccgacccc
      361 cgatcagcac cgacccgagg ttcgtcaact gccgagggta gccgtccgcg tagccgaccg
      421 gcacggtggc gagccaggca tcgcgttcga gccggtaccg gtgcccgtag gagacggcct
      481 cgccgacagt gagccgcttc acggcgctca cccgactccg ccaggtgagc gccggtcgca
      541 gaccgagatg cgcgccgatc cccggcgccg gttcgatccc gtacagggcg attccgggcc
      601 gcacgaggtc caggtgggac tccggatgca ggatcgtcgc cccggagttc gctgcgtgga
      661 ggacccgggc acgcacgcgg gcggtccgag cggtctcggc ggcgtcgagg aatcgatcga
      721 gctgttcctt ggtcgtggcc tcgtcctcct ccgaccgagc gaagtgcgtg aagagaccct
      781 cgatctcgaa gccggcctcc tgcacgcgtc gggcgaacgc cggcgcgtcc tccggtggcc
      841 agacccctac gcggtgcatc ccggtatcga ccttgacgtg taccggcacc gatcccctcg
      901 tcgcggacgc gagacgctcg agccctccgt ccgaatacaa cgtcggcgtg agccggtgcg
      961 cgagcgcgac ggcttccgaa ccggccggga gctccgagag cacgaggacc ggcgtctcga
     1021 tccccgcgga acggagccga agcccttcct cgacgagcgc caccccgcac cacgtggcgc
     1081 cggcctcgat cgcggcgcgg gccacctcca cgtcgccgtg cccgtagccg ttcgccttca
     1141 ccacggccat cagctcggcg gtctcgggtt tgaggacgcg ggcgttgtgt cgtatcgcct
     1201 cgagatccac ctcgacccag gtgggtcgat agccttcgct cactcggcct ccacgtgtgc
     1261 gaccgcctca ggcacacgct ccacgacatc ccccgcgacc gctccttcgc cgaggtccct
     1321 gcccgcgaac agcccggcca ggccgtggag gtaggcggcc gcggcggccg actcggtcgg
     1381 cgccacgccg cgcgcgagga gtccgccgat catcccggtc aggacgtcac ccgtgccggc
     1441 ggtcgcgagc acgggagacc cggtcacgtt cacgaacaac cggccgtcgg aattcgcgat
     1501 cacggtgcga ctccctttaa ggagtgtcac cgcacccgtt tggttcgcca gcgcccggac
     1561 gtgcgtgggg cggtcggcgt cgaggtcccg agccttcacc ccagtcaacc ggccgaactc
     1621 gccgacgtgc ggcgtgagga cggcctcgga cgcacggtcc ccgagcgcgg ccccgtcggc
     1681 cgtgaacgcg ttcagaccgt cggcgtcgag gacgagaggg accggcgagc ggcgcacgat
     1741 gtcgcggacg aaggccaccg tcgcctggtc ggtcgtgagg cccggcccga tcgccagcgc
     1801 gtcggcggcc tccagccgtt ccagcacggg tgcggccgcc tccgcagcca cggtgccgtc
     1861 cgccgtctcg ggaagcggta ggaacgtggg ctcggtcagt cccgcctgga tctgcgacag
     1921 gcagccctca ggggcggcta cgacgaccag ccccgctccg accctgcccc cggccatcgc
     1981 gatcaatcgc gccgctccgg tcatcgcgcg cgaccccgcg accaccacga gcacccccga
     2041 agcgcgcttg tgcgtgtcga tgtcacgttt gggcagccac cctgccacgt cgtccggctc
     2101 cgtgagccag gcgcgagccc ggagaaggtc gtcggggaag ccgatgtcga caacccgcag
     2161 gatgcctgca tgctccgcgc cgggcatcag gacggtgccc agcttgggtg cgccgagcgc
     2221 aacggtcaga tccgcacgga cggcctctcc ctccaccgct cccgtcgccc catcgacgcc
     2281 ggacgggata tcgaccgcca ccaccggtgc gtcggcctcg ttcaggctcg cgatcgccgt
     2341 cgcccagtcg tcctccggca tgccgcggaa ccccgtcccg aagatcgcat ccaccacgat
     2401 gtccgcgcgg acgagctcgc gccggagggc gtgttcgtcg aacgtccgtg cgcggatgcc
     2461 cgggaccgcg gcgaggcgcc gagcgttcga ggccgcagga tcgcggagct cgtcgggatc
     2521 cccgagcagc acgacggtcg tccgtacacc gttgcgagcg aggtgacgcg tcgcgacgag
     2581 tccgtcgccc ccgttgttgc ccttcccgca gacgaccacc gcccggcgcc cgtacgtgcc
     2641 accggcgagc tcgcgtgtgg ctcgcgccac cgcacggccc gcccgctcca tcagcgcctc
     2701 ggccgcgatc ccgcgcgact gggtgtcgcg gtcaagggcg gcggcctcct ccggcgtcag
     2761 gaccggcttc atcggaccgc gatcgcgaac gccaccgcgt tcgttcgctc gtgcgtgaac
     2821 gtgatcagca cctgggtgac gccgagggcg tccgcccgac gcttggcgtt gccgtcgagc
     2881 cggatcgcgg gcgcgcctga cgggtgccgg gtcacgctga tgtcctgcca gcggcggccc
     2941 cggtagccgc cgagcgcctt gatcgtggct tcgcgggcgg agaaccgccc tgcgtaggac
     3001 tctgcggggc gggccttcga gtcgcagtac gcgatctcct cgggcgtgaa gacgcgatca
     3061 cggaacgtcg ggtgtcgagc gatcgctcgc tccatccgcg cgatctcgca cagatcgacc
     3121 cccagcccga cgatgtccac ccgagaaggc tacgccacgt ggctcccacc ggtagttgcg
     3181 gcggggcgcc gtcgagcgaa ccacacgagt gcgcccagcg cgatgaccac caccagggcg
     3241 cctaccaggg ccaccttcga ctcgatgctc cacgctgttc ctatcggagc gatcgtggag
     3301 accggcggat ggctgttctg cgcgaaggac aggagctggt tcggcggcac ttgctgacac
     3361 aagctcgact cccatacgcc atcgttcgct cgatcgagga gcaatccggc gcgagggtcg
     3421 tcgaagagtt cgatcccgca cgaggcccca tccccgctgg tacggacgat cgcggtgggg
     3481 ccgaacggtc ctttgatgac acgttcgacg tcgaacgtcc aggccgaacg accatcgctc
     3541 agaggatcac gatccacata tgtcccgatg aaggctccgt cagcctctgg gagccgggaa
     3601 ccgaggtcca tctcggcgca cgaacaggcg cgtgccggtg atgcgacgag gaggccggcc
     3661 gcgacggtca ggatgagaac gatcgcgtga ccgagtcgag ccggacggtg catcgcccgg
     3721 gtcatgccgc gacgctactc gaccgtgacg ctcttcgcga ggttccgtgg ctggtcgacg
     3781 tcggatccgc gcaagcgggc gacgtggtag gccaacagct gcagcggcac cgtcaccacg
     3841 accggcgaaa gcagctccgg tgtctccgga acctcgagta cgtgctgggc cacaccgcgg
     3901 atctgctcgt ccccctccgt cgcgacggcg atcacctcgg cgccacgcgc gcggacctcc
     3961 tggatgttcg agagcatctt cgggtagacg tggcacgtgg tcgcgaccgc caccacgggc
     4021 accccgtcct cgaccagtgc gatcgggccg tgcttcagct cgccggccgg atagccctcc
     4081 gcgtgcacgt agctgatctc cttcagcttc agagcccctt cgagggccgc ggggaagccg
     4141 gtgtgccgac cgatgaacag ccagtctcgc gcgtcttgga accgccgagc gagctcctgg
     4201 acctgcgcgt ccagcgcgat cgcccgttcg acctgcaccg ggagctccgc catccgtgtc
     4261 agtacctctg cgatctcttc ggggaacatc gagccgcgga cctgcgcgag gtacatcgcg
     4321 aggatgtact gcgacaccat ctgggtcgcg aacgtcttcg tcgcggcgac accgatctcc
     4381 gggcccgcat gcatgtagat cacgccgtcg gcctcccggg cgagcgacga gcccacggtg
     4441 ttggtgacgg ccaggagagg cgatccctgg tgtcgcgcat gccgggcggc ctcgagcgtg
     4501 tcgatggtct cccccgactg cgagaccgcg agggtgagcg tgtcggggcc gaggaccggg
     4561 tcgcgataac ggaactcgct ggcgatgtcg atctccacgg gcagccgcgt ccagtgctcg
     4621 atcgcgtact tcgccacgag gcccgagtga taggccgtcc cgcacgcgac cacgaagacc
     4681 ttgttcacct cccggaggac gtcgtcggag atcttcaggt cgtcgagcgc gaggcggcgc
     4741 gactcgtcga cccgccccac cagcgtgtcc cggatcgcgc tcggttgctc gtggatctcc
     4801 ttgagcatga aggtgtcgaa gccgcccttc tccgcgcgag cggtgtccca gtcgacctcg
     4861 atggactccg gatgcacgac tgcgccgtcg aagtccgaga acgtcgcccc gtctgctcgc
     4921 acctcgacga tccgtccctc gtccacgggg atcaccgtgg tcgtgcgtcc gagcacggcc
     4981 gggatgtcgg aggcgaggat gctttcgccg gcgccgacac cgacgaccaa tggggaagcg
     5041 accttcacgc cgacgaggag accgggctcc tccgacgaca tgacgacgag cgagtacgcc
     5101 ccttccagct cgcggaccac cggcgcacaa gctgttcgta cgcagtca
//
LOCUS       DASUVE010000173         3506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1593939, whole genome shotgun sequence.
ACCESSION   DASUVE010000173 DASUVE010000000
VERSION     DASUVE010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593939"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VF036_03705"
     CDS             complement(<1..696)
                     /locus_tag="VF036_03705"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX6264368.1"
                     /translation="MSPAPRYLLAFAVPLVVVLVVTPVAGRLARRLGILDHPSEQKFH
                     HDPTPYLGGAAVAAGLVAIGFLTTGTSAQVFVLLLGGLVLGVWGLLDDWVTVRPPVKL
                     LVEASAGVALWFSDVRAGLFGNEVLDLALTVLWVVAVTNALNLLDNMDGLTAGVSTVA
                     ALAFFTIAAARGDHLVASFALAVAGASLGFLFHNLPPATIFLGDAGSLMLGFLLAALG
                     LKLDLVGENGFVRS"
     gene            complement(696..1679)
                     /locus_tag="VF036_03710"
     CDS             complement(696..1679)
                     /locus_tag="VF036_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6264369.1"
                     /translation="MEYLITGGAGFIGSHLADALLARGDSVLALDDLSTGAEANVRHL
                     FDHPGFELREGSILDHDLVSKLGNNVDVIVHLAAAVGVKLIVEHPLDSLLTNIRGTEI
                     VLDAAAELGCKVLITSTSEIYGKNASGPLKEDDDRILGSPFKARWSYSTSKAVDEILA
                     RGYWRDRGTPTVVVRLFNTVGPRQTGAYGMVVPRFVHQALRGEPITVYGDGEQRRSFC
                     HVSDVVTALLQLLDHPGAIGDVFNVGARNEVSINELARLILAATDSSSPVVHISYDVA
                     YEEGFEDMERRIPDTGKLEALTGWRATRSLEDVIEDVIEHERVGAAPKRAR"
     gene            complement(1791..2195)
                     /locus_tag="VF036_03715"
     CDS             complement(1791..2195)
                     /locus_tag="VF036_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264370.1"
                     /translation="MRRSTILRASALALVALLAVEGSAFAAFVEPTSLFIHRRPSGSV
                     QQGTEITIRGRLSSDERSCRRHKVIRLVKIGEGVVDLDTTNRFGRYVFERRARRTQMF
                     RTRFDGTASGVHPDIEVCLASSSRRITVRVRP"
     gene            2242..>3506
                     /locus_tag="VF036_03720"
     CDS             2242..>3506
                     /locus_tag="VF036_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HEX6264371.1"
                     /translation="MRTQLARRSYRIWQWILVLTVVVVVLTVGDALWVGVRAAGDLRD
                     ARIGLRDGRDALLAGDIGVARARFEAAADAAASASGALDEPAADVASWLPVIGDDVDA
                     VRALARAASSSADAGGALSGAAEAVGWDGESVPGYEPGAVDLDAAEAARVDLERSPQL
                     LGEAHATLAEVDPSGLVGPLREWFVGARAELTRSARLARRAAAVATLLPRFLGGEEGR
                     SYLLMAMNLSDPRGSGGYPGSYGLIRANEGRIRVTDFAPTGTLGSVKPVDPPRPDARR
                     YARFGALTHFISTTYPPDWPTSAELFLRMWEASGRSPVDGAIGVDAVFLSQLLAAAED
                     PVSVPVWPEEITADNAVRILGRETFETTRTEESNRLQAAIGGALWEAVLSRPLDPEAL
                     VSAMSRSVVERHLQVYVRDPEDQAVLHELG"
BASE COUNT          566 a         1206 c         1208 g          526 t
ORIGIN      
        1 ggatcgcacg aagccgttct cgccgaccag atcgagcttc aggccgagag ccgccaggag
       61 gaacccgagc atcaacgagc ccgcgtcccc caagaagatc gtcgccggcg gaaggttgtg
      121 gaacaggaat cccaggctcg ccccagcgac cgcgagcgcg aacgacgcca ccaggtgatc
      181 gccccgcgcc gccgcgatcg tgaagaaagc gagggcggcc accgtgctca cgccagccgt
      241 gagcccgtcc atgttgtcca gcaggttcag cgcgttcgtg accgcgacca cccacaggac
      301 ggtgagcgcg aggtcgagca cctcgttgcc gaacaggccc gctcggacgt ccgagaacca
      361 cagcgccacc cccgccgacg cctccacgag gagcttcacg gggggacgga cggtcaccca
      421 atcgtccagc agtccccata cgccgaggac cagtccgccg aggaggagca cgaagacctg
      481 agcggacgtg ccggtcgtca ggaagccgat ggccacgagc ccggccgcga cggctgcgcc
      541 accgaggtac ggggttggat cgtgatggaa cttctgctcc gaagggtggt ccaggatgcc
      601 gagccgccgg gcgagtcgcc cggcgaccgg ggtcacgacg agaacgacga cgagcgggac
      661 ggcgaaggcg agcaggtagc ggggcgccgg actcatcacc tggctctctt cggcgccgct
      721 ccgactcgct cgtgttcgat cacgtcctcg atcacgtcct cgagcgacct cgtggcacgc
      781 cacccggtca gcgcctcgag ctttcccgta tccgggatcc gccgctccat gtcctcgaac
      841 ccctcctcgt atgcgacgtc gtaggagatg tgcacgaccg gggaggacga atcggtggcg
      901 gccaggatca gccgggcgag ctcgttgatc gacacctcgt tccgcgcccc gacgttgaac
      961 acgtcaccga tggctccggg atggtcgagc agctggagga gcgcggtcac cacgtcggag
     1021 acgtggcaga acgaccggcg ctgctccccg tcgccgtaga ccgtgatggg ctcaccccgg
     1081 agggcctggt gcacgaagcg ggggacgacc atcccgtagg cgcccgtctg gcgagggcca
     1141 accgtgttga acagccggac cacgacggtc ggcgtccctc gatccctcca gtagccgcgg
     1201 gcgaggatct cgtcgaccgc tttggaggtg ctgtaggacc accgagcctt gaacggggat
     1261 ccgaggatcc ggtcgtcgtc ttctttgagg gggccgctcg cgttcttccc gtagatctcg
     1321 gaggtcgacg tgatgaggac cttgcagcca agctcggcgg ccgcgtcgag gacgatctcg
     1381 gtgccccgga tgttcgtgag gagggaatcg agaggatgct ccacgatgag cttgaccccg
     1441 acggcggccg ccaggtggac gatcacgtcc acgttgttcc ccagcttcga gacgaggtcg
     1501 tgatccaaga tcgacccctc gcgcagctcg aagcccgggt ggtcgaacag atgccggacg
     1561 ttcgcctccg cgccggttga aaggtcgtcg agcgcgagga cggagtcacc acgggcgagg
     1621 agcgcatcgg ccagatgtga cccgatgaag ccggcgccac cggtgatcag atattccatc
     1681 gcgtcgccct ccacgcctca cgccggtccc ccgaccggga tgcgctcatg ctagttccgt
     1741 tgcagggccg tgcaacaacg ctctctcggc ttcccggggg gctcgtctgt ctagggccgg
     1801 acgcggacgg tgatccttcg ggagctcgag gccaggcaca cctcgatgtc cgggtgcacg
     1861 ccactcgcgg tcccatcgaa cctcgtccgg aacatctgcg tgcggcgggc acgccgttcg
     1921 aacacgtatc ggccgaagcg gttcgtggta tcgaggtcga ccacgccttc gccgatcttc
     1981 acgagcctga tcaccttgtg cctcctgcag gaacgttcgt ccgaagacag acgaccgcgg
     2041 atcgtgatct ccgtcccctg ttggacgctc cccgagggac ggcgatggat gaacagagac
     2101 gtcggttcta cgaaggcggc gaacgcgcta ccctcgacgg cgaggagtgc gacgagggcg
     2161 agagcggatg cgcggaggat cgtgcttcgt ctcatgctgc accccatccc ggagactatg
     2221 agcacagact aggcccattc catgaggacg caactcgctc gacggtccta ccgcatctgg
     2281 cagtggatcc tcgtcctcac tgtcgtggtc gtggtgctga ccgtcggcga tgcgctctgg
     2341 gtcggcgttc gtgctgccgg cgacctccgc gacgcgcgga tcggtctgcg ggacggccgt
     2401 gacgcactct tggccggaga catcggggtg gcccgcgcga gattcgaggc ggccgccgac
     2461 gccgctgcct cggccagcgg agcgctcgac gaacccgcgg cggacgtggc gagctggctg
     2521 ccggtgatcg gcgacgacgt cgatgccgtc cgggcgctcg cgcgcgccgc atccagctcc
     2581 gccgacgcgg gcggcgccct gagcggcgct gcggaagccg tcggctggga tggggagagt
     2641 gtcccgggct atgaacccgg cgccgtcgac ctcgacgccg ccgaagccgc gcgggtggat
     2701 ctcgagcgtt cgccccagct cttaggcgag gcacacgcaa cgctcgcgga ggtcgatccc
     2761 agcggtctcg tcggcccgct gcgagagtgg ttcgtggggg ctcgcgccga gctcacgcgg
     2821 agcgctcgcc tcgcccgtcg agcggcagcg gtcgcgacgc tcctgccgag gttcctcggc
     2881 ggcgaggagg ggcggagcta cctcctgatg gcgatgaacc tctcggatcc tcgcggctcc
     2941 ggcggctacc cgggcagcta cgggctcatc cgtgccaacg agggcaggat ccgggtgacc
     3001 gacttcgccc cgacggggac gctcggcagc gtcaaacccg tcgacccgcc gaggcccgac
     3061 gcgcgacgat acgcccgctt cggggcactc acgcacttca tcagcacgac ctaccctccg
     3121 gactggccga cctcggccga gctcttcctc cggatgtggg aggcgagcgg acgctcgccg
     3181 gtggacggtg cgatcggcgt cgacgccgtg ttcctctcgc agctgctcgc ggcggcggag
     3241 gaccccgtct cggtccccgt gtggccggag gagatcaccg ccgacaacgc cgtccgcatc
     3301 ctcggccgcg agacgttcga gacgacgcga acggaggagt cgaaccgtct ccaagccgcg
     3361 atcggcggcg cactgtggga ggccgtcctg tcccgtcccc ttgaccccga ggcgctcgtc
     3421 tcggcgatga gccgatcggt cgtcgagcgg catctccagg tctacgtgcg ggatcctgaa
     3481 gaccaagccg ttcttcacga gctcgg
//
LOCUS       DASUVE010000174         2976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1029637, whole genome shotgun sequence.
ACCESSION   DASUVE010000174 DASUVE010000000
VERSION     DASUVE010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029637"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /gene="ilvC"
                     /locus_tag="VF036_03725"
     CDS             <1..670
                     /gene="ilvC"
                     /locus_tag="VF036_03725"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX6264372.1"
                     /translation="TVTPPPDVDVTMIAPKGPGHLVRRTYEEGIGTPALVAVHQDATG
                     KARQRALAYGHGIGAARAGIIETTFKEETETDLFGEQTVLCGGLSELIRSGFDTLVAA
                     GYQPEIAYFECLHEVKLIVDLIYEGGLSWMRHSISDTAEWGDYVSGARVIDDGTRETM
                     RQLLGEIQDGSFAKRWIAEADGGFPEFLAKRREARASQLEDVGRQLRTMMPWLQSEDK
                     VPPS"
     gene            634..2196
                     /locus_tag="VF036_03730"
     CDS             634..2196
                     /locus_tag="VF036_03730"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX6264373.1"
                     /translation="MAAERGQGAAVVSDPDVVRFFDTTLRDGEQAPGIALSKDEKVEI
                     AEQLARLQVDILEAGFAISSDGEFDAVREIATSVKGPVIASLARVVPRDIERAAEALK
                     GADRFRIHVFISTSQIHMEDMLRMSHRQVLDGIAEGVKLAAGYTDDVEFSAQDATRTE
                     LDFLLECLDVAVKAGATTINVPDTVGYATPAEFGELVRIVRDRTPDHVTISTHCHNDL
                     GLAVANSLAGVENGARQVEVAVNGIGERAGNCSLEEMAMILRTRREALGVRHGLHLKE
                     IVRTSRLVSALTGYSVQPNKAVVGASAFAHESGIHQHGVLANRATYEIMDPIEIGLEG
                     NQIVLGKHSGRHAFADALDKVGISIDDDHMHRAFARFKELADRKIQITDQDLAAIVSE
                     EVGSGKEDLLVLESLASGGGTHLAPTATVRLRRGDEVIEESAMGDGMIDAACGAIQRA
                     AGVEATLITFNVSSVTGGSDALGDVVVQLEIDGRRVTGRGVATDVVEASARAYLAAIN
                     RAASVAASEVTS"
     gene            2193..>2976
                     /locus_tag="VF036_03735"
     CDS             2193..>2976
                     /locus_tag="VF036_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX6264374.1"
                     /translation="MTAAVATEVRPERVGAGMRPYRIGVIRGDGTGPEVVAEALKVLD
                     AVRGDVAVELVEFDIGADRYLRTGETLPDEELATMRGLDAILLGAVGDPRVPPGVLER
                     DLLLRIRFELDQYVNLRPVIRFPGVQTLVPSHTEDDVNMVVIRENSEGLYSGVGGFHR
                     KGTRHEVAVQSSVNTRHGVERIVRYAFEHAMLPGRRRHVTLVHKTNVLTFAGDLYQRV
                     VDELAAAFPDVETEYVHVDAACIYFLDSPSRFDVIVTDNMFGD"
BASE COUNT          471 a          989 c         1077 g          439 t
ORIGIN      
        1 gacggtcacc cccccgcccg acgtcgacgt cacgatgatc gcgccgaaag ggcccggcca
       61 cctcgtgcgg cgcacctacg aagagggcat cgggactccc gcgctcgtcg cggtccacca
      121 ggatgccacc gggaaggccc gccagcgcgc gctcgcctac ggccacggga tcggcgccgc
      181 ccgcgcgggc atcatcgaga cgacgttcaa ggaagagacc gagaccgacc tgttcggcga
      241 gcagacggtg ctgtgcggcg ggttgtccga gctgatccgc tccggcttcg acacgctcgt
      301 ggcggccggc tatcagccgg agatcgctta cttcgagtgc ctccacgagg tgaagctgat
      361 cgtcgacctg atctacgagg ggggtctctc ctggatgcgc cactccatct cggacaccgc
      421 tgagtggggt gactacgtct ccggcgcgcg cgtgatcgac gacggcacgc gcgagacgat
      481 gcggcagctc ctcggagaga tccaggacgg atccttcgcg aagcgctgga tcgccgaggc
      541 cgacggcggc ttcccggagt tcctggccaa gcggcgcgag gcgcgagcgt cccagctcga
      601 ggacgtcggt cggcagctgc ggacgatgat gccgtggctg cagagcgagg acaaggtgcc
      661 gccgtcgtga gcgaccccga cgtagtccgc ttcttcgaca ccaccctgcg cgacggggag
      721 caggcccccg gcatcgcgct gtccaaggac gagaaggtcg agatcgcgga gcagctcgcc
      781 cggctgcagg tcgacatcct ggaggcgggc ttcgcgatct cctccgacgg tgagttcgat
      841 gccgtgcgcg agatcgcgac gagcgtgaag ggccccgtca tcgcgtcgct cgctcgcgtg
      901 gtcccccgcg acatcgagcg cgccgccgag gcgctgaagg gcgccgaccg gttccggatc
      961 cacgtcttca tctcgacgtc gcagatccac atggaagaca tgctccggat gtcccatcgg
     1021 caggtcctcg acgggatcgc cgaaggggtg aagctcgcgg cgggctacac cgacgacgtg
     1081 gagttctcag cccaggacgc cacacgaacg gagctggact tcctcctcga gtgcctcgac
     1141 gtcgcggtga aggccggggc gacgaccatc aacgtccccg acacggtcgg gtacgccacg
     1201 cccgcggagt tcggcgagct cgtccggatc gtgcgggatc ggaccccgga tcacgtgacc
     1261 atctccaccc actgccacaa cgacctgggg ctcgcggtcg cgaattcgct ggccggcgtg
     1321 gagaacggtg cgcgccaggt ggaggtcgcg gtgaacggga tcggcgagcg cgcggggaac
     1381 tgctcgctcg aggagatggc gatgatcctg cgcacgcgcc gcgaggcgct cggcgtgcgc
     1441 cacgggttgc acctgaagga gatcgtgcgg acgtcgcggc tcgtctcggc gctcaccggg
     1501 tactcggtgc agccgaacaa ggcggtggtg ggggcgagcg cgttcgcgca cgagagcggc
     1561 atccaccagc acggcgtgct cgccaaccgc gccacgtacg agatcatgga tccgatcgag
     1621 atcgggctcg agggcaatca gatcgtcctg gggaagcact ccgggcgcca cgcgttcgcc
     1681 gacgcgctcg acaaggtggg gatctcgatc gacgacgacc acatgcaccg cgcgttcgcg
     1741 cgcttcaagg agctcgccga ccgcaagatc cagatcaccg accaggatct cgccgcgatc
     1801 gtgtccgagg aggtcggcag cgggaaggag gatcttctcg tcctcgagtc cctggcctcg
     1861 ggaggcggca cgcacctggc gccgacggcg accgtgaggc tccgccgcgg tgacgaggtg
     1921 atcgaggaat ccgcgatggg cgacgggatg atcgacgccg cctgcggcgc gatccagcga
     1981 gcggcgggcg tggaggcgac gctgatcacg ttcaacgtgt cgtcggtcac gggtggctcg
     2041 gacgcattgg gagatgtcgt cgtccagctg gagatcgacg ggcggcgggt tacggggcgc
     2101 ggtgtcgcca ccgatgtcgt ggaggcgagc gcgagggctt acctcgccgc gatcaaccgc
     2161 gcggcctcgg tcgcggcgag cgaggtcacg tcgtgacggc tgcggtcgcc acggaggttc
     2221 ggcccgagcg cgtcggcgcc ggcatgcgcc cctaccggat cggcgtgatc cgtggggacg
     2281 ggaccggccc ggaggtcgtc gccgaggccc tcaaggtcct cgacgccgtg cggggggacg
     2341 tcgccgtcga gctcgtggag ttcgacatcg gcgcggaccg ctacctccgg accggcgaga
     2401 ccctgcccga tgaggaactc gcgacgatgc gcggtctcga cgcgatcctc ctgggtgcgg
     2461 tgggcgaccc gcgcgttccg cccggggtgc tcgagcgcga cctgttgctg cggatccgct
     2521 tcgagctcga ccagtacgtg aacctgcgtc ccgtgatccg gttccccggc gtgcagacgc
     2581 tcgtgccgtc ccacaccgag gacgacgtga acatggtcgt gatccgggag aactccgagg
     2641 gcctctacag cggcgtgggc gggttccatc ggaaggggac gcgtcacgag gtggccgtgc
     2701 aatcctcggt caacacccgc catggcgtgg agcggatcgt gcggtacgcg ttcgagcacg
     2761 cgatgctgcc gggccggcgg cgtcacgtga cgctcgtcca caagacgaac gtgctgacgt
     2821 tcgcgggcga cctgtaccag cgggtggtcg atgagctcgc cgcggcgttc ccggacgtcg
     2881 agaccgagta cgtgcacgtg gacgccgcct gcatctactt cctggactcg ccctcgcgct
     2941 tcgacgtgat cgtgaccgac aacatgttcg gtgaca
//
LOCUS       DASUVE010000175         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_276680, whole genome shotgun sequence.
ACCESSION   DASUVE010000175 DASUVE010000000
VERSION     DASUVE010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276680"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VF036_03740"
     CDS             complement(<1..912)
                     /locus_tag="VF036_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264375.1"
                     /translation="MASEPTPSGRRRPRTRGRRVALGALAGLAAFAALLVLDGIWAGK
                     SLVRGLTAARSELSIAIESIVTGDPGSAGPHFDAASRAADGARRAVEHPSMGIAGLLP
                     IAGDNIDAAAAVADASRATAEAGTEMVRVARTLGWTDIRIPASTAAGDVDIDAVEDAI
                     PDMEAVTRRLDVALRALEEAGSDGLLGPVESGYRDAVDSLTLRLDLAIRFRDSLRLAA
                     AMFGGEHRYLVYVPTLGIPRPGGGAPATVGVLIARDGSLALESITPAPSELIEAEISM
                     DWRRTARALLEAAEASGLTDLDGVIQID"
     gene            1163..>2222
                     /locus_tag="VF036_03745"
     CDS             1163..>2222
                     /locus_tag="VF036_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019852806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264376.1"
                     /translation="MPDQLVPVRNRVSFAKLDEVLQLPDLVAIQKDSFDRLLNEGLKE
                     VLEEVSPIEDFTEQFQLYFGKHEFRAMKFSEEECRDKDITFSAPLFVEATFVNKVTGE
                     IKEQEVFLGDFPMMTDRGTFIINGTERVVVSQLVRSPGVYFSKEPDKTTDKDIFIAKI
                     IPSRGAWLEFDVDKKDTVGVRIDRKRRQHITVFLKALGFSAAEILKLFDNAVSIQNTL
                     EKDHFETQEEALEDIYRKLRPGEPPTAESAKTLLENLFFNPKRYDVARVGRHKVDKKL
                     GAAEGELLKQLRSHHKGLGELDNPDRKAWEQIRYRVFSSMQKEEAAGQGAPKYKAVLT
                     FEDILKTVGYLVHLHAGEA"
BASE COUNT          385 a          768 c          723 g          346 t
ORIGIN      
        1 atcgatctgg atgaccccgt cgagatcggt cagaccggac gcttcggccg cctcgagaag
       61 ggctcgcgcc gtcctcctcc agtccatcga gatctccgct tcgatcaact cgctcggtgc
      121 gggcgtgatc gactcgagtg cgagggagcc gtcacgtgcg atcaggacgc cgaccgtcgc
      181 gggcgccccg cctccgggcc gtgggatgcc cagcgtgggg acgtagacga ggtaccgatg
      241 ctcccctccg aacatcgcgg ccgcgagccg caacgagtcc ctgaatcgga tcgcgaggtc
      301 gagccggagg gtgagcgagt cgacggcgtc ccgatagccg gactcgacgg ggccgagcag
      361 tccgtcgctc cccgcctcct ccagcgcccg gagagcgacg tcgagccgtc gcgtcaccgc
      421 ctccatgtcg gggatcgcat cctccacggc gtcgatgtcg acgtcccccg cggcggtaga
      481 agcagggatc ctgatgtcgg tccacccgag cgtgcgcgca acccggacca tctcggtccc
      541 ggcctcggcc gtcgcccggg aggcgtcggc gacggctgcc gcggcgtcga tgttgtcgcc
      601 cgcgatgggc aggagcccgg cgatgcccat cgaggggtgc tccaccgccc tccgggcgcc
      661 atccgccgcc cggctggcgg cgtcgaagtg tggccccgcc gaccccgggt ctcccgtgac
      721 gatcgactcg atcgcgatcg agagctccga ccgtgcggcc gtgagcccac ggacgaggct
      781 ctttcccgcc cagatgccgt ccaggacgag gagggcggcg aacgcggcga ggcccgccag
      841 ggcgccgagg gcaacccgtc tcccgcgggt ccttggtcgc cggcggccgg acggggtggg
      901 ctccgaggcc atcgccgccg accctaccgt cggtacgcac ccgacaccat caagtcctca
      961 gccggcgggg gtcggaaggc gtccagggaa ggccgttcgg cggcttgacc ctcggcctcg
     1021 aggaggcgta ccctcggggc agccgccgtg tccagggcag gcaccgttga cggtgcctgc
     1081 tcctcctcgt agactgctcg tttgccgtgc cgtccaccgc gctctgccgt gcgctctcga
     1141 cgtctcccgg gaggggttct gcttgcctga ccagctcgtg cctgttcgga atcgtgtcag
     1201 tttcgccaag ctcgacgagg tcctccagct tcccgacctc gttgccatcc agaaggactc
     1261 attcgatcgg ctcctgaacg agggcctcaa ggaggttctc gaggaagtct cacctatcga
     1321 agacttcaca gagcagttcc agctctattt cggtaagcac gaattccgag cgatgaagtt
     1381 ctcggaagag gaatgtcgcg acaaggacat cacgttctcc gctcctctgt tcgtcgaagc
     1441 aaccttcgtc aacaaagtca ccggtgagat caaggagcag gaggtcttcc tgggcgactt
     1501 cccgatgatg accgaccgcg gcaccttcat catcaacggc accgagcgcg tcgtggtctc
     1561 tcagctggtg cgctcgccgg gcgtctactt cagcaaggag cccgacaaga ccaccgacaa
     1621 ggacatcttc atcgcgaaga tcatcccgtc gcggggagcc tggctcgagt tcgacgtgga
     1681 caagaaggac accgtcggcg tacggatcga tcgaaagcgg cggcagcaca tcaccgtctt
     1741 cttgaaggcc ctcggcttca gcgcggccga gatcctgaag ctcttcgaca acgcggtctc
     1801 catccagaac accctcgaga aggaccactt cgagacccag gaggaggccc tcgaggacat
     1861 ctaccggaag ctgcgacccg gcgagccgcc cacggccgag agcgccaaga ccctcctcga
     1921 gaacctgttc ttcaacccca agcgctacga cgtcgcgcgg gtgggacgcc acaaggtgga
     1981 caagaagctg ggtgccgctg agggcgaact gctgaagcag ctgcgctccc accacaaggg
     2041 cctgggcgag ctcgacaacc cggatcgcaa ggcatgggag cagatccgct atcgggtctt
     2101 ctcctcgatg cagaaggagg aggcggccgg acagggggcg cccaagtaca aggcggtcct
     2161 cacgttcgag gacatcctca agacggtcgg ctacctcgtg cacctgcacg ccggcgaggc
     2221 cg
//
LOCUS       DASUVE010000176         4437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_276847, whole genome shotgun sequence.
ACCESSION   DASUVE010000176 DASUVE010000000
VERSION     DASUVE010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276847"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..1686
                     /gene="dnaA"
                     /locus_tag="VF036_03750"
     CDS             265..1686
                     /gene="dnaA"
                     /locus_tag="VF036_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX6264377.1"
                     /translation="MAVALPDQEARRAWSAVLDRARTELPETTFLMWFTDVRPIALRD
                     QVLSLAVPSQFVRERLQHNHLGLIEETAEEAFGRPIKIEMDVDEGIRHEEGPLVDITT
                     TPPAAYAAIGAPATPPTMGKPLGAPGLPFPNYTFDAFVPGPSSRFAHAAAMAVAEAPL
                     STAYNPLFIYGGTGLGKTHLLVAIGHHIWRLMPDTRIKYVTSEQFVTEFIKAVRERQG
                     DAFRHKYREVDILLVDDIQFLAKREETQTEFFHTFNHLHGAGRQIVIASDRPPHELSG
                     LEERLVSRFRWGLCVDVQPPDLETRIAILQLKAERENIPAVVPEVIEFVASKFDQSVR
                     ELEGALVRVVAWSELTRQPIDIELAERALQDLIPQTEHEIPPPLILEETATYFQLSTG
                     DLVSKSRSRPLTQARHIAMYLMRECTGLSLVKIGEIFGGRDHTTALHGIKKVEDEMRS
                     RDDTFRQVQDLTRIIRGRVRGLV"
     gene            1911..3011
                     /gene="dnaN"
                     /locus_tag="VF036_03755"
     CDS             1911..3011
                     /gene="dnaN"
                     /locus_tag="VF036_03755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX6264378.1"
                     /translation="MKFRCDRDDLSEALQTVQRGVSSRPGIPALTGVLLEAADDGTLM
                     LTTTDLEVSARLSIAVQVQEAGAALVPARLLADTVKSLSEAPVDFETDQSQAHIRCAA
                     YEGALRLLPVDDFPGLQEPSGTAISVEPAAFAEAVAQVARAASKDEARPVLTGVLLEI
                     SREGVVLVATDSYRLAVRDLVATADGEAKAIVPERALTEAGRAAVDQKAGVEASVNAS
                     QISFRVGSLTLTSRLIEGEFPNYRQLLPETVESRLTISRQQLLEAVRRVGLLARDTTP
                     VRLEFNALGVKLSSSSPDLGQAVETVEARYEGEDLVAAFNPQYLVDGLTAATGESVRI
                     DVRDGLKPGVIRGEGDELTYLVMPVRLPAAVG"
     gene            3036..4124
                     /gene="recF"
                     /locus_tag="VF036_03760"
     CDS             3036..4124
                     /gene="recF"
                     /locus_tag="VF036_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEX6264379.1"
                     /translation="MRLEWLELRDFRNHRRTAVDRVADGLTVVVGPNGEGKTNLLEGM
                     FFLYALRSPRSSTNAPLVREGTDVAYARGEFTGIDGKALVEVEIPRKGASRVKLNKSA
                     LRRKRELRRQVRSVLFGPFDLPVVIGDPSKRRDLMDDAIVALWPLKEGLSAAYERALR
                     QRNRLLKEWDGHRGGPSGLDAWDEELVRTGVPVIEARGDAVARLAIGASVEFEALSGY
                     ELLVTYAPNVTGASEEAFRELLDARRSDELLRRTSLVGPHRDDLTLAVRDLGARAFGS
                     HGETWAAALCLRLGLAAAVAAETGEPPLLLVDDPFSALDPDRRDRIARGLADRGGQVV
                     ISVADEADVPPASSAVWDVAGGAVAVRG"
     gene            4124..>4437
                     /locus_tag="VF036_03765"
     CDS             4124..>4437
                     /locus_tag="VF036_03765"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX6264380.1"
                     /translation="MPSAKGFPRRKDGPSEEVVELGEVIDNLLSEQVFARGLPVAQLA
                     ASWSSIVGERLASETAPAALEAGVLTVTATSGPWGAQARFLHEEIRRRADEALGGDQI
                     TG"
BASE COUNT          707 a         1527 c         1507 g          696 t
ORIGIN      
        1 cttggccatc ctcggattcc ttcgcggaag gaaggccggc aacgccgccg gccgaagggg
       61 aagtatacgg aaacatccct gggtggaggg gttgacaaca gaaagtgcgt caagacgctt
      121 ccggccactc gcttggggaa aaaccccggt gctatagtcc ggcccgcact tccccggggt
      181 gaggggcccc cggcatcccc cgaccccgcg gcctccccgg attcgggtta tccaccggtg
      241 tggaaaggct ctgtggaatc tcagatggcc gtcgctttgc ccgatcagga agcaagaagg
      301 gcctggtcgg ccgtgctgga tcgtgctcgc acggagctgc cggagaccac gttcctgatg
      361 tggttcaccg atgtccggcc gatcgcgctc cgcgatcagg tgctctccct cgcggtcccg
      421 agtcagttcg tccgggaacg ccttcagcac aatcatcttg ggctcatcga agagaccgct
      481 gaggaggcgt tcggccgccc catcaagatc gagatggacg tggacgaagg catccgccac
      541 gaggagggcc cgttggtgga catcaccacc acgcccccgg ccgcctacgc cgcgatcggt
      601 gcaccggcca cgccgccgac gatgggcaag cccctcggcg ctcccggtct cccgttcccc
      661 aactacacgt tcgacgcgtt cgtccccggc ccatcgagcc ggttcgcgca cgccgccgcc
      721 atggcggtcg cggaggctcc gctgtcgacc gcgtacaacc ccttgttcat ctacgggggc
      781 accggactcg gtaagacgca cctcctggtc gcgatcgggc atcacatctg gcgattgatg
      841 cccgacacgc ggatcaagta cgtgacctcg gagcaattcg tcacggagtt catcaaggcc
      901 gtccgcgaac ggcagggtga cgcgttccgg cacaagtacc gagaggtcga catcctgctc
      961 gtcgacgaca tccagttcct cgccaagcgc gaggaaacgc agaccgagtt cttccacacg
     1021 ttcaaccacc tccacggcgc cggccgccag atcgtgatcg cgtcggaccg gccgccgcac
     1081 gagctgtccg gcctcgagga acggctggtg agtcggttcc ggtggggact gtgcgtggac
     1141 gtgcagccgc cggacctcga gacgcgcatc gcgatcctcc agctgaaggc cgagcgggag
     1201 aacatccccg ccgtcgtgcc cgaggtgatc gagttcgtcg cgtcgaagtt cgaccagtcc
     1261 gtccgagagc tcgaaggcgc cctcgtgcgc gtggtcgcgt ggtccgagct cacccgccag
     1321 ccgatcgata tcgagctcgc ggagcgagcc ttgcaggacc tgatcccaca gaccgagcac
     1381 gagatcccac cgccgctcat cctggaagag accgcgacct acttccagct ctcgaccggg
     1441 gacctcgtgt cgaagtcgcg ctcgcgaccg ctcacgcagg cgagacacat cgccatgtac
     1501 ctgatgcgcg aatgcacggg cctatctctg gtcaagatcg gcgagatctt cggcggacgc
     1561 gaccacacca ccgccctcca cggcatcaag aaggtcgagg acgagatgcg gtcccgcgac
     1621 gacacgttcc gtcaggtcca ggacctcacg cggatcatcc gcggccgtgt tcgggggttg
     1681 gtgtgatgtc ggtggacaac ttcgcggaga tggccgttct cgaacccgga acgaccgatc
     1741 caggaagagc gccaaggacc ctccaccggt cgtccccatg tcggacagcc gcgctacgct
     1801 gcgcgaacac catatttctc cacttctcca ccgtccctac taccgctctt cctgtatatg
     1861 aacctaagaa gcaacagcct gggaacaggc cacgaccggg aggttgatgg atgaagttcc
     1921 gatgcgaccg cgacgacctg tccgaggcgt tgcagaccgt tcagcgcggc gtgtcctcgc
     1981 gccccgggat cccggcactc accggcgtgc tgctcgaggc cgcggacgac ggcaccctga
     2041 tgctcacgac gacggacctc gaggtctcgg cacggctctc gatcgccgtc caggtccagg
     2101 aggccggcgc ggccttggtg ccggctcgcc tgctggcgga caccgtgaag tcgctctccg
     2161 aagccccggt cgacttcgag acggaccagt cccaggcgca catccgctgc gcggcatacg
     2221 agggcgctct ccgactgctc cccgtcgacg acttcccggg tctccaggag ccgtcgggga
     2281 ccgcgatctc ggtcgagccg gccgcgttcg ccgaggccgt cgctcaggtg gcccgagcgg
     2341 cctcgaagga cgaggccagg ccggtgctca cgggtgtgtt gctcgagatc agccgggagg
     2401 gagtggtcct cgtcgccaca gactcgtacc ggctcgccgt ccgtgacctc gtcgccaccg
     2461 ccgacggtga ggcgaaggcg atcgtccccg agcgcgcgct gaccgaggcc ggtcgtgcgg
     2521 ccgtcgacca gaaggcggga gtggaagcgt cggtcaacgc ctcgcagatc tcgttccgcg
     2581 tcggctccct cacgctgacg tctcgcctga tcgaagggga gttcccgaac taccgccagt
     2641 tgctgccgga gaccgtggag agccgattga cgatctcgcg tcaacaactc ctcgaggccg
     2701 tccgtcgggt ggggctcctc gctcgtgaca cgacccccgt gcgcctggag ttcaacgcgc
     2761 tcggcgtgaa gctgtcgtcg tccagcccgg acctcggtca agccgtcgag acggtcgagg
     2821 cccggtacga gggggaggac ctcgtcgcgg cgttcaaccc gcagtacctc gtcgacggct
     2881 tgaccgcagc caccggcgag agcgtgcgga tcgacgtgcg cgacgggctc aagcccggcg
     2941 tgatccgcgg cgagggtgac gagctgacct acctcgtgat gccggtccgg ctgcccgccg
     3001 cggtcggctg aaccgccggc cggtcgcgaa cccggatgcg cctggagtgg ctcgagctcc
     3061 gggatttccg gaaccaccgc cgcactgccg tcgatcgcgt cgcggacggt ctgacggtgg
     3121 tcgtgggacc gaacggcgag gggaagacca acctgctgga gggcatgttc ttcctgtacg
     3181 cgctccgctc gccccgctcc tccacgaacg ccccgctcgt ccgcgaggga accgacgtgg
     3241 cgtacgcgcg aggtgagttc accggcatcg acggcaaggc cctggtcgag gtggagatcc
     3301 cccggaaggg tgcgagccgg gtgaagttga acaagagcgc gctccgacgc aagcgcgagc
     3361 tccgccgtca ggtgcgctcg gtcctcttcg gtcccttcga cctgcccgtg gtgatcggtg
     3421 acccctcgaa gcggcgagac ctgatggacg acgcgatcgt ggcgctgtgg ccgttgaagg
     3481 aagggctgtc cgccgcgtac gagcgcgcgc tccgtcagcg caaccggttg ctcaaggaat
     3541 gggacgggca tcgcggtggc ccatccgggc tcgacgcgtg ggacgaagag ctcgtgcgga
     3601 cgggcgtgcc ggtgatcgag gcacgcggag atgccgtcgc acgcctcgcc atcggtgcct
     3661 ccgtcgagtt cgaggcactt tcgggctacg agctcctcgt gacgtacgcc ccgaacgtga
     3721 cgggcgcctc ggaggaggcg ttccgggagc tgctggacgc acggcggtcc gacgagctgc
     3781 tccgcaggac gtccctcgtc gggccgcatc gcgacgacct caccctcgct gtgcgcgatc
     3841 tcggagcccg tgcgttcggt tcgcacggtg agacgtgggc cgctgccctg tgcctgcgcc
     3901 tcggcctcgc ggcggccgtc gccgccgaga cgggcgaacc gccgctcctg ctcgtggacg
     3961 atccgttcag cgccctcgac cccgatcgcc gagaccggat cgcgcggggg ctggcggatc
     4021 gcggcggcca ggtcgtgatc agcgtggcgg acgaggccga tgtaccgcct gcgtcgtccg
     4081 cggtgtggga cgtggctggg ggagccgtgg cggtgcgagg ctgatgccga gcgcgaaggg
     4141 gttcccgcga cggaaggacg gaccgagcga ggaggtcgtc gagctcggtg aggtgatcga
     4201 caacctcctg tccgaacagg tcttcgcccg aggcttgccg gtggctcagc tcgccgcttc
     4261 gtggtcgtcg atcgtcggcg aacggctggc gtcggagacc gctccggcgg ccctcgaagc
     4321 gggcgtcctc accgtcacgg cgacctccgg gccatggggc gctcaggccc gattcctcca
     4381 cgaagagatc cgccgtcggg cggacgaagc gctcggcggc gatcagatca ccgggat
//
LOCUS       DASUVE010000177         3484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_276944, whole genome shotgun sequence.
ACCESSION   DASUVE010000177 DASUVE010000000
VERSION     DASUVE010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276944"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..958
                     /locus_tag="VF036_03770"
     CDS             266..958
                     /locus_tag="VF036_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX6264381.1"
                     /translation="MLDRDVVRSNVDEVRSRIAEAADRSGRDPADVTLVAATKTVKVE
                     RIRWAVDAGVRAIGENYVSELRRKRQEIAGVAWHFIGTLQSGTASQVADHADVVESVA
                     GERAARRLAGRAARSGRTLDVLIEVDLTEGRAGVRPDDLPAFADLVASLGGVRLIGLM
                     TVPPIPRTPEDSRPSFARLRELREATQERHPDVLESSMGMSLDYGVAVEEGATIVRIG
                     TALFGSRATRPR"
     gene            999..1466
                     /gene="sepF"
                     /locus_tag="VF036_03775"
     CDS             999..1466
                     /gene="sepF"
                     /locus_tag="VF036_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010473429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEX6264382.1"
                     /translation="MTGVWKKTLNYLGLVEDDEEFVEELPDVEPSPVRRMRPHPVREV
                     SSEAEGVVRTIAAPRPAGGSSVIHKSEPKRFNEAREVADRFKEGTAVIMNLQSTEDST
                     ARRLVDFASGLVYGLDGKIELVANRVYLLTPADVEVSAEERERIAGGGFYNQF"
     gene            1537..1794
                     /locus_tag="VF036_03780"
     CDS             1537..1794
                     /locus_tag="VF036_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX6264383.1"
                     /translation="MTEIICLALLVAMIVLFIRVIVDWVVLAGWRPPHSGPIRAALDV
                     TVDVTEPPLRLLRRIVPPTGGFDLSDLVAFVSLTVLRTVVC"
     gene            1810..2577
                     /locus_tag="VF036_03785"
     CDS             1810..2577
                     /locus_tag="VF036_03785"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX6264384.1"
                     /translation="MKKKDAATESGSEWSGPTGNRVSPLDVQQKEFHVTRVGVGYRMR
                     EVDEFLDQVTDTLSALIAENEQLRASSGGASATGAPDARARETTANPADRAAVDAFLQ
                     REKAFLQNLGGLVQAHAEELRSMIRATRDARPQVVMTREEPAPEQEPAPERESVPERE
                     LVVEQEPVAESVAEAEPVTEEPVTEGEEPPPTVRVEEAPSSDPTSSDEEEDDEPAATT
                     GVIAEEPIRLEDPEPARSRRSEEKEGDSLRELFWGEE"
     gene            complement(2600..>3484)
                     /locus_tag="VF036_03790"
     CDS             complement(2600..>3484)
                     /locus_tag="VF036_03790"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HEX6264385.1"
                     /translation="VTTATLLAPFTPFVAEEVWSNIAADRAGRPDSVHLADYPEVHEA
                     VIDPGLDEAMVLARRVVELGRRIRTETRVRTRQPLARAVVHLPVGAQDFEQLLGVVAE
                     ELNVKRVDLAGSAESFGRWRAKPDFQVLGPRLGARVRAAASALAEDDGSLAARLAAGE
                     QVEISIEEGSPIVIGPGDVALSQDVRAGYGVATEAGITVALELDVTRELRREGVAREL
                     VRMIQDARREAGLEVTDRIDLAVETSDEPAEALAAHRDEIAEETLASELRTEGIDGFR
                     QASELDGVQVVVTLKRTT"
BASE COUNT          545 a         1176 c         1251 g          512 t
ORIGIN      
        1 cgaggaggag gaaccgctcc cgtcgatcct ggccgaggac gacgaggacc gggtcttcac
       61 gaacggcgag cagtccccac cggtcagttt cgacgcgcag gaggatctgg acatcccgga
      121 cttcctgaag agctgaacgt cgaccccgcg cggcccctcg tccggggcgg ggaggtccga
      181 gtgaaggagg cgcccttccc ccgcctcctc tcggaggatc tctccgcccc gttcgtgtcc
      241 cggggccgga cgctcggagg cctcgatgct cgatcgtgac gtggttcgca gcaacgtcga
      301 cgaggtgcga tcgcggatcg cggaggccgc ggatcgctcg gggcgggatc ccgcggacgt
      361 cacgctcgtc gccgcgacga agaccgtcaa ggtcgagcgg atccgttggg ccgtcgacgc
      421 cggcgtccgc gcgatcgggg agaactacgt gtcggaactc cggcggaaac gccaggagat
      481 cgcgggggtc gcctggcact tcatcgggac cctccagtcg ggcaccgcat cgcaggtcgc
      541 cgatcacgcc gacgtcgtgg aatcggtcgc gggagagcgc gccgcgagac gtctcgccgg
      601 tcgcgccgcg cgctccggtc ggacgctcga cgtgttgatc gaggtggacc tcacggaggg
      661 ccgggcgggc gtgcgacccg acgacctgcc cgccttcgcg gatctcgtcg cgtcgctcgg
      721 gggcgtgcgc ttgatcggcc tcatgaccgt tcccccgatc ccgcgtaccc ccgaagactc
      781 ccgtccttcc ttcgcacggc tccgcgagct gcgtgaggcg acccaggaac gacatccgga
      841 tgtgctggaa tcgtcgatgg ggatgtcgtt ggattacggg gtcgcagtgg aagaaggcgc
      901 tacgattgtg cgcatcggga cggcgctgtt cgggtcccga gcgaccaggc cccgatgaac
      961 gaaccgggga acgggccggg tgacgaggag gaaccgacat gacgggcgtg tggaagaaga
     1021 cgctgaacta tctcggtctg gtcgaggacg acgaggagtt cgtcgaggag ctgccagatg
     1081 tggagccttc gccggtgcgt cgcatgcgcc ctcatcccgt ccgggaggtg tcctccgaag
     1141 cggaaggcgt ggtgcgcacg atcgcggcac cccgtcctgc cggcggatcg tccgtgatcc
     1201 acaagtcgga gcccaagcgg ttcaacgagg ctcgtgaggt cgccgaccgg ttcaaggaag
     1261 gcaccgccgt catcatgaac ctgcagagca ccgaggattc gaccgcacgg aggctcgtgg
     1321 atttcgcgtc gggcctcgtc tacggcctcg acggcaagat cgagctcgtg gcgaaccgcg
     1381 tgtacctgct cacgccggcc gatgtcgagg tctcggcgga ggagcgcgag cgcatcgcgg
     1441 gtgggggctt ctacaaccag ttctgaagcg cacgcgagac cgtgacctgc agcgccgctg
     1501 ctagcatcgc gcctcgatga cgagcggtgg tgcctcttga cggagatcat ctgcctcgcc
     1561 ctcctcgtcg cgatgatcgt cctgttcatc cgggtgatcg tggactgggt ggtgctggca
     1621 ggatggcgcc caccgcactc ggggccgatc cgggccgcgc tcgacgtcac cgtcgacgtg
     1681 acggagccgc cgctccggct gctccgacgc atcgtaccgc cgaccggtgg gttcgatctc
     1741 tccgacctgg ttgcgttcgt ctcgctcacc gtgttgcgta cagtggtctg ctgacgatga
     1801 gagggtgaga tgaagaagaa agacgcggcg accgagagcg gatcggagtg gagcgggccg
     1861 accggcaacc gcgtgagccc gctcgatgtc cagcagaagg agttccacgt cacgcgggtg
     1921 ggtgtcggct atcggatgcg tgaggtcgac gagttcctcg atcaggtgac cgacaccctg
     1981 tcggcgctga tcgcggagaa cgagcagctg cgagcgtcga gcgggggcgc ctccgccacg
     2041 ggcgcgccgg atgcccgcgc acgagagacg acggcgaacc ccgccgatcg ggcggcggtg
     2101 gatgccttcc tccaacggga gaaggcgttc ctccagaacc tcggcggact cgtgcaggcg
     2161 cacgcggagg agctgcggtc catgatccgc gcgacccgcg acgcgcgccc gcaggtggtc
     2221 atgacccgag aggagcccgc gcccgagcag gagcccgcgc ctgagcggga gtccgtgcct
     2281 gagcgggagc tggtggtcga gcaggagccc gtcgccgagt ccgtcgcgga agccgagccg
     2341 gtgacggaag agccggtgac ggaaggcgag gaaccccctc ctacggtgcg cgtcgaggag
     2401 gcaccctcgt ccgatccgac ctcgtccgac gaggaagaag acgatgagcc cgctgcgacc
     2461 accggggtga tcgccgagga accgatcagg ctcgaggatc ccgagccggc acgatcacgt
     2521 cggtcggagg agaaggaagg ggattcgctc cgggagctct tctggggcga ggagtagccc
     2581 ggccgggacg aggcctcggt cacgtcgttc gcttgagggt caccacgacc tggacgccgt
     2641 cgagctccga ggcctggcgg aagccgtcga tgccctccgt gcgcagctcg gacgcgagcg
     2701 tctcctcggc gatctcgtcg cgatgtgcgg ccagcgcctc ggcgggctcg tctgaggtct
     2761 ccaccgccag gtcgatacgg tcggtcacct cgagcccggc ttccctccgc gcgtcctgga
     2821 tcatgcgcac cagctcgcgc gccaccccct cgcgccggag ctcgcgcgtc acgtcgagct
     2881 cgagggcgac cgtgatgcct gcctcggttg cgacgccgta tcccgcgcgc acgtcctggc
     2941 tcagggcgac atcgccggga ccgatcacga tcggcgaccc ctcctcgatg gagatctcca
     3001 cctgctcgcc ggccgccagc cgagccgcga gcgagccgtc gtcctcggcc aaggcggacg
     3061 ccgcagcgcg cacgcgggca ccgaggcgtg ggccgagcac ctggaagtcg ggtttcgctc
     3121 gccaccgacc gaacgactcg gccgaccccg cgaggtcgac gcgcttcacg ttcagctcct
     3181 ctgcgacgac gcccagcagc tgctcgaaat cctgcgcgcc gacaggcagg tggaccaccg
     3241 cccgcgcgag gggctgccgg gttcgcaccc gcgtctcggt ccggatgcgc cggccgagtt
     3301 cgaccacacg ccgtgccaac accatcgcct cgtcgagtcc gggatcgatg accgcctcgt
     3361 gcacctcggg gtagtcggcg agatggaccg agtccgggcg accggcccgg tcggccgcga
     3421 tgttcgacca gacctcctcg gccacgaacg gcgtgaaggg ggccaggagc gtcgcggtcg
     3481 tcac
//
LOCUS       DASUVE010000178         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_654574, whole genome shotgun sequence.
ACCESSION   DASUVE010000178 DASUVE010000000
VERSION     DASUVE010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654574"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VF036_03795"
     CDS             <1..1277
                     /locus_tag="VF036_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6264386.1"
                     /translation="PYHHLTVDAHLTGSLRAMGRLLAGEGADDDPVQAEAVEQVSDRD
                     PLLLGALLHDIGKNGEGGHVPVGARVVVSILDRMGIELPARDLVRFLVERHLILPDTA
                     TRRDLSDENLVLDVAAVVGSPDRLAALYLLAKADAEATGPAAWTSWRRTLIHELVAKV
                     EHVLERGEMGTAIAEQLADRIELIRGILDDEPGEEVDRFLRRMPRAYLLVADPGRVAR
                     HYATIAPAVGAKDVRAVHFDGTRPGTYEVLVVARDRAGLLSWIAGSLALAGMSILTAH
                     VFTTDDGVAADLFEVEGVWEPEVPERRWREFRGMLRRAVDGSISLERRVDEKRRWYPA
                     PRVPTPVTVTVDNEASDFSTVIEVGAPDRLGLLYDITRTFAELGIDVRLAKVATYEGR
                     VIDSFYVRDSLGPKLSDDLDTLEKALRHRLES"
     gene            complement(1337..>2041)
                     /locus_tag="VF036_03800"
     CDS             complement(1337..>2041)
                     /locus_tag="VF036_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6264387.1"
                     /translation="FSFDGWKHGVFYPEGLKNREMLTYYSQQLSSVEINYTFRRFPTA
                     KSLTTWREQAAEGFTFTLKANQRITHFKRLADTDEDVRAFLELANLLGDKLGCVLFQC
                     PPSLHYDRALIEAFVGYLPPGGRFAMEFRHPSWIEAKDLLLSQGVAWCVAETDEKDPG
                     PDDLSWEPIGYLRLRKTEYPDEELRAWADRIAPALDAGADVFCYFKHEEDGAGPKMAK
                     RLREALEDRAQDRASA"
BASE COUNT          310 a          690 c          716 g          325 t
ORIGIN      
        1 atccgtacca ccacctcacg gtggacgccc atctcacggg gtcgctccgc gcgatgggcc
       61 ggctcctcgc gggcgagggg gcggacgacg atcctgtcca ggccgaggcc gtcgagcaag
      121 tctccgatcg cgatccgctg ctcctcgggg cgctcctgca cgacatcggc aagaacgggg
      181 agggtgggca cgtgcccgtc ggcgcgcggg tcgtcgtgtc gatcctcgac cggatgggta
      241 tcgagctccc agcccgcgac ctcgtccgtt tcctggtcga acggcacctg atcctgccgg
      301 acaccgcgac ccgccgggat ctgtccgacg agaacctcgt gctcgacgtc gcagcggtgg
      361 tgggttctcc cgatcgcctc gccgcgctct acctgctggc caaggcagat gccgaggcga
      421 ccggtcccgc cgcgtggacg tcatggagac ggacgctcat ccacgagctc gtggcgaagg
      481 tcgagcacgt cctggagcgc ggcgagatgg ggaccgcgat cgcggaacag ctcgccgaca
      541 ggatcgagct gatccgcggc atcctcgacg acgagccggg ggaggaggtc gatcgcttcc
      601 tccggcggat gccgcgcgcc tacctcctgg tcgccgatcc gggccgggtc gctcggcact
      661 acgcgacgat cgcgccggcc gtcggcgcca aggacgtccg agccgttcac ttcgatggga
      721 cgcgacccgg cacgtacgag gtgctcgtcg tcgccaggga tcgcgcgggg ctcctgtcgt
      781 ggatcgcggg gtccctcgcc ctcgcgggca tgtcgatcct caccgcccac gtgttcacga
      841 ccgatgacgg ggtcgccgcc gacctcttcg aggtggaggg ggtgtgggag cccgaggtgc
      901 ccgagcgccg ctggcgcgag ttccgcggga tgctccgtcg ggcggtcgac ggctcgatct
      961 cgctcgaacg gagggtggac gagaagcgcc gctggtaccc ggcaccaagg gtgccgacac
     1021 ccgtcactgt cacggtcgac aacgaggcct ccgacttctc gaccgtgatc gaggtcggcg
     1081 cccccgaccg gctcggtctc ctgtacgaca tcacgaggac cttcgccgag ctcggcatcg
     1141 acgtgcgcct ggcgaaggtg gcgacctacg aaggaagggt catcgactcc ttctacgtcc
     1201 gggactcgct cggtcccaag ctctcggacg atctcgacac gctcgagaag gcgttgcgcc
     1261 atcggctgga atcgtgactc cgttcgacgg agttccggcg aggaccccgg acggggtcgc
     1321 tcgaacgagg gaagggtcag gcagacgcgc ggtcctgagc cctgtcctcc aacgcctcgc
     1381 gcagccgctt cgccatcttc gggcccgcgc cgtcctcctc gtgcttgaag tagcagaaga
     1441 cgtcggcgcc cgcgtcgagc gcgggggcga tccgatcggc ccacgcacgg agctcctcgt
     1501 ccgggtactc ggtcttgcgc aggcgcaggt agccgatcgg ctcccaggag aggtcgtcgg
     1561 gccctgggtc cttctcgtcg gtctcggcga cgcaccacgc caccccttgg gacagcaaca
     1621 ggtccttcgc ctcgatccac gagggatggc ggaattccat cgcgaaccgt ccgcctggag
     1681 ggaggtagcc gacgaaggcc tcgatcaggg cacggtcgta gtgcagcgag ggcggacatt
     1741 ggaacaggac gcagccgagc ttgtcgccga ggaggttcgc gagctcgagg aaggcgcgga
     1801 cgtcctcgtc cgtgtcggcg aggcgcttga agtgggtgat gcgctggttc gccttcagcg
     1861 tgaacgtgaa cccctccgcc gcctgctcgc gccacgtcgt gagcgacttc gccgtgggga
     1921 accggcggaa ggtgtagttg atctcgacgg acgacagctg ctgggagtag taggtcagca
     1981 tctcgcggtt cttgagcccc tccggataga agacgccgtg cttccagccg tcgaacgaga
     2041 a
//
LOCUS       DASUVE010000179         5968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1410561, whole genome shotgun sequence.
ACCESSION   DASUVE010000179 DASUVE010000000
VERSION     DASUVE010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410561"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VF036_03805"
     CDS             complement(<1..191)
                     /locus_tag="VF036_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264388.1"
                     /translation="MTITVVGGEPSGLASMVADLIEQNLARDPARMALLRRSVAVLDA
                     PDADVTVFLRIERDGVRVG"
     gene            complement(188..1228)
                     /locus_tag="VF036_03810"
     CDS             complement(188..1228)
                     /locus_tag="VF036_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6264389.1"
                     /translation="MRGVVFDGVGAVRVADLPDPDLEAPTDAIVRVTRSAICGSDLHL
                     LHGKAPMEPGEPLGHEAVGVIEAVGDGVETRRVGDRVAVAFNVACGHCWFCGIGQSSL
                     CDEGKIFGYGIFGDALPGAQAERLRVPDADLNLLEIPDGVGDEAAVFVGDVLTTGFYG
                     ASLTSAGPDDVVAVLGCGPVGFCTIQGLRTHGGPTIYALDRDPSRLALAKIAGAIPVH
                     IDERNPVTALAEATDGRGADVAIDAVGHPSAFEGAIDTVRRGGTVVVLGVYSSETTEL
                     QLGAYWSRALTLRFAGLTPVVSWWGRALEGLERGAVDPTPLISHRLPLEAAAEGYALF
                     DSREATKVVLEP"
     gene            complement(1228..1647)
                     /locus_tag="VF036_03815"
     CDS             complement(1228..1647)
                     /locus_tag="VF036_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX6264390.1"
                     /translation="MPEFPTEEWFQAYIRAVDASEEYAEYAATWEGDAVIHVEAEPDK
                     GVATDVRGLLDLWHGGCRGGGIVDEARAASAEFVIRAPYSRWKDVIRGDLEPVKGLVQ
                     GKLRVRGDLPKILRYTKGTQELAYIAGQVDTTFPDEP"
     gene            complement(1649..2422)
                     /locus_tag="VF036_03820"
     CDS             complement(1649..2422)
                     /locus_tag="VF036_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX6264391.1"
                     /translation="MNAPLPPPPDELLVEHAPPRATWRWWEAVIVTLLGFVLGTLVSV
                     PVFLALGGASGQRMNGPGAAAGAVTYLVLVISLVVWLQVAHRGWWRSIGWPARGKRLH
                     EAASGVGWGFASQIGVTVVAYFVVSALAAISGRDVEVPRQVDPSLTGWAVVALVVYAV
                     VMAPLAEELVFRGLIFHSVADRRGFWPGAFASAIPFGLIHVVPGGALDVSVLVFMMMI
                     TGLVWAWIHWRHHNLLVNIATHAAFNAVGVVFALQLGGF"
     gene            complement(2419..3945)
                     /locus_tag="VF036_03825"
     CDS             complement(2419..3945)
                     /locus_tag="VF036_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264392.1"
                     /translation="MNGRTGSEDAMADVLEDENGEPISPPGLYNARVGDLGIGPSVDG
                     LVAEGLAVADGPTLVVIVSRSPLITGRNEGDELGAVVAAWVDPDEMFDAAGQLIVTDE
                     EPKALTSDSTRRAGVVADVDPAATVAEWLDLPYDAGAPIERTDEPAPLDLYERYLQQR
                     RLAVPIAAASWGVMLLFGLAGLLALAFRRSASPRTLAVAGALVGSLPWLALGLLLVGH
                     VPSLTYATVVPSLLLIVVVGVAFTRWVEARRGIFVSVAACGAVILVILGIEAALGWPA
                     AATPLAGGGQLDGGRFFGMPNVEIGIVLGSAMFLAHRLRVSSGVLLLAACALVAGSPW
                     TGSNFGAAITLFAAAGMWPAIQRRRPWWMIAVITGVTTAVGTAAVALMHRHLTGRSTH
                     VTAFLEETDGVMGAIERQLDRLGVGLDLIAGNPLAAIPVVGTLVLLLIVLRPWPAIAR
                     SFEGHDAWRDAVLVILLGSVIAYLAEDTGAAAVGFAFGFALSGLLGVSLAAARGKMSP
                     "
     gene            complement(4116..5384)
                     /gene="murA"
                     /locus_tag="VF036_03830"
     CDS             complement(4116..5384)
                     /gene="murA"
                     /locus_tag="VF036_03830"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX6264393.1"
                     /translation="MDRLFVTGGARLTGSVRISGAKNSALKLMAASLLAPGRSALRNV
                     PRIQDCFTMGEVLEQLGARVSWEDGGVAVDATHLSSVEAPYELVRRMRASIAVLGPLL
                     ARTGEARVAMPGGDNIGSRPIDLHLDGLRRMGARIDAEHGFLVATADGLTAAQITLDY
                     PSVGATENLLMAAVIADGVSVIDNAAREPEIADLAAFLAAMGAGIHGAGSATIEIEGV
                     GALEPAEHTVICDRIEAGTWAAAAVATRGDLVLDGARPEHLELFLEKLGEGGAEIRRT
                     DDGLRVRQPERARAADFVTLPYPGIATDFQPILLAVLSTANGTSIATENVFEGRFTYV
                     DELRRMGADIRTEGHHAVIRGVERLSAAPVRAMDIRAGAAMAIAALTADGVTEIAAIE
                     HVDRGYEDFEAKLIGLGAEVRRDPELVRLP"
     gene            complement(5434..5835)
                     /gene="atpC"
                     /locus_tag="VF036_03835"
     CDS             complement(5434..5835)
                     /gene="atpC"
                     /locus_tag="VF036_03835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX6264394.1"
                     /translation="MALEVHVVTPEREVWSGQATEVIAHGVDGDVGILAGHAPLLVHL
                     AIGPLQIQEEDGTWLKAVVDGGFLHVSTEEGASRVDVLATNAQLEAEIDVDAVRRRLE
                     GLRSSAEGEDDEQVKTEIARAEARLTLIGSD"
BASE COUNT          972 a         2197 c         2010 g          789 t
ORIGIN      
        1 tcgccgaccc ggacaccgtc gcgttcgatc cggaggaaga ccgtcacgtc ggcgtcgggc
       61 gcatcgagca ccgcgaccga cctgcgcagg agcgccatcc gtgcgggatc gcgcgcgagg
      121 ttctgctcga tcaggtcggc gaccatcgac gcgaggccgc tcggctcgcc gccgaccacc
      181 gtgatcgtca cggctcgagg accaccttcg tggcttcccg actgtcgaac agggcgtagc
      241 cctcggcggc tgcctcgagc gggagccgat ggctgatcag cggcgtcggg tccaccgcgc
      301 cacgctccag tccctccagc gcccggcccc accacgacac cacaggcgtc agccccgcga
      361 accggagcgt gagcgctcgc gaccagtacg ccccgagctg gagctccgtc gtctccgacg
      421 agtagacgcc caggacgacc accgtgccgc cgcgccggac ggtgtcgatc gcgccctcga
      481 acgcactcgg atggccgacc gcgtcgatcg cgacgtcggc ccctcgcccg tcggtcgcct
      541 ccgcgagcgc ggtcacgggg ttccgctcgt cgatgtgcac cgggatcgcg cccgcgatct
      601 tcgccagcgc cagccgcgac ggatctcggt cgagcgcgta gatcgtcggg ccgccatggg
      661 ttcgaagacc ctggatcgtg cagaagccca ccgggccgca gccgagcacc gcgacgacgt
      721 cgtccgggcc ggcgctcgtc agggaagccc cgtaaaaccc ggtcgtcagc acgtctccga
      781 cgaagacggc ggcctcgtcc cccaccccgt ccgggatctc cagcaggttc aggtcggcgt
      841 cggggacccg gagacgctcg gcctgggctc cggggagggc atcgccgaag atcccgtagc
      901 cgaagatctt gccctcatcg cacagcgagg actgcccgat cccacagaac cagcaatggc
      961 cgcaggcgac gttgaacgcg acggcgacgc gatccccgac ccgccgggtc tccaccccgt
     1021 cgcctaccgc ctcgatcacg ccgacggcct cgtgtcccag cggctccccc ggttccatcg
     1081 gcgccttccc gtggagcagg tgaaggtcgc tcccacagat cgccgagcgg gtgacacgga
     1141 cgatcgcgtc ggtcggcgcc tcgagatccg gatcgggcaa gtcggcgacg cggacggcgc
     1201 ccaccccgtc gaacacgacc ccccgcatca gggttcgtcg gggaaggtcg tgtccacctg
     1261 accggcgatg taggccagtt cctgtgtgcc cttcgtgtag cgcaggatct tcgggagatc
     1321 gccgcggaca cggagcttcc cctggacgag ccccttcacg ggttcgaggt ccccgcggat
     1381 cacgtccttc caccgcgagt acggcgcgcg gatcacgaac tcggccgagg ccgccctcgc
     1441 ctcgtcgacg atgccgccgc cgcgacaccc gccgtgccag aggtcgagga gcccgcgcac
     1501 atcggtcgcg acacccttgt ccggctctgc ctcgacatgg atcaccgcgt cgccctccca
     1561 ggtcgccgcg tattcggcgt actcctccga cgcatcgacc gctcggatgt aggcctggaa
     1621 ccactcctcg gtcgggaact cgggcatctc agaacccccc cagctgcaac gcgaagacga
     1681 ccccgaccgc gttgaacgcg gcatgcgtcg cgatattcac gaggaggttg tgatggcgcc
     1741 agtgtatcca ggcccagacc agaccggtga tcatcatcat gaacaccagc acgctcacat
     1801 cgagcgcccc gcccgggacc acgtggatga gcccgaacgg gatggcggag gcgaacgcgc
     1861 ccggccagaa cccgcgacgg tcggcgacgg agtggaagat cagaccccgg aagaccagct
     1921 cctcggcgag gggtgccatc acgacggcat agacgaccag ggcgaccacc gcccatcccg
     1981 tgagcgaagg atcgacctgc cgagggacct cgacgtcgcg tccggagatc gcggcgagtg
     2041 ccgagacgac gaagtacgcc accaccgtga cgccgatctg ggaggcgaac ccccatccga
     2101 cgcccgacgc cgcctcatgg aggcgctttc cgcgcgccgg ccacccgatc gagcgccacc
     2161 agccccggtg cgccacctgg agccagacga cgagggagat gacgagcacg aggtacgtca
     2221 ccgcccccgc ggccgcgccg ggcccgttca tccgttgccc cgaggcaccg ccgagcgcga
     2281 ggaacacggg gacggagacg agcgtcccaa ggacgaatcc caggagcgtc acgatgacgg
     2341 cctcccacca ccgccacgtg gctctcggcg gggcatgctc gacgagcagc tcgtcgggag
     2401 gcggtgggag cggtgcgttc atggcgacat cttcccgcga gcggcggcca gagacacccc
     2461 gaggagcccc gacagcgcga acccgaacgc gaaaccgacg gccgccgcgc cggtgtcctc
     2521 cgcgagatag gcgatcaccg aacccagcag gatcaccagc acggcgtcgc gccacgcgtc
     2581 gtgtccttcg aagctccggg cgatcgcggg ccacgggcga agtacgatga ggagcaggac
     2641 cagcgtgccc accaccggga tcgctgcgag cgggttgccg gcgatcagat cgagccccac
     2701 gcccagccgg tcgagctgtc gctcgatggc gcccatgaca ccgtcggtct cctcgaggaa
     2761 cgccgtgacg tgggtggacc gaccggtcag atgccggtgc atcaacgcga ccgcggccgt
     2821 cccgacggcc gtggtcactc ccgtgatcac ggcgatcatc caccatggcc ggcggcgttg
     2881 gatcgccggc cacatgccgg cggccgcgaa gagtgtgatc gcggcgccga agttggatcc
     2941 ggtccacggc gagccagcga cgagcgcgca tgccgcgaga aggagcacgc cgctgctcac
     3001 gcgaaggcga tgcgcgagga acatcgcgga tcccagcacg atcccgatct cgacgttcgg
     3061 catcccgaag aagcgccccc catcgagctg tccaccgccc gccagaggag tagccgctgc
     3121 gggccatccg agcgccgcct cgatcccgag gatcaccagg atcacggcac cgcaggcggc
     3181 gacggaaacg aagatccccc ggcgcgcctc cacccacctg gtgaacgcga cgccgaccac
     3241 gacgatcagg agcagggacg gcaccacggt cgcgtaggtg agggagggga catggcccac
     3301 cagcaacagg ccgagcgcga gccaaggcag cgagccgacg agcgccccgg cgacagcgag
     3361 cgtgcgggga gacgcactcc gtcggaacgc cagagcgagg agccccgcga gaccgaagag
     3421 caacatcacg ccccagctcg cggcggcgat cggcacggcg agccggcgct gctggaggta
     3481 gcgctcgtag aggtccaggg gcgctggttc gtcggtgcgt tcgatcggcg cgccggcgtc
     3541 gtacgggaga tccagccact cggcgacggt cgccgcgggg tcaacgtcgg cgacgacgcc
     3601 agcccgccgg gtagagtcgg aggtcagggc cttcggctcc tcgtccgtca cgatcagctg
     3661 acctgccgca tcgaacatct cgtccggatc cacccatgca gcgacgacgg ccccgagctc
     3721 gtcgccttcg ttccttccgg tgatcagcgg agagcgggac acgatcacga cgagtgtcgg
     3781 gccatccgcg acggcgagtc cctccgccac gagtccgtcc acggaaggac cgatcccgag
     3841 gtcaccgacg cgagcgttgt acaggcccgg aggcgagatc ggttcgccgt tctcgtcctc
     3901 gagcacgtcg gccatcgcgt cctccgagcc tgtgcgaccg ttcatgaggg cggatccgcc
     3961 caaagcagcc aacgttctga tctccgagac gttgaggaga tcccggaggg acgcgtccgc
     4021 cgtctcgatg aggatcgcgc tccggcgccc tacttgtgcg tgcgccggag ctgacaggag
     4081 cgccagaccg gcgcacaccg cgacgagcgc tcgcctcacg ggaggcggac gagctcgggg
     4141 tcgcgccgga cctcggcgcc cagaccgatg agcttcgcct cgaagtcctc gtagccccga
     4201 tcgacgtgct cgatagcggc gatctccgtc accccgtcgg cggtcagtgc ggcgatcgcc
     4261 atcgcggcgc cggcgcggat gtccatggct cgcacgggtg cggcggagag gcgctcgacg
     4321 ccccggatca ccgcgtggtg cccctcggtg cggatgtcgg ctcccatgcg ccggagctcg
     4381 tcgacgtacg tgaaccgccc ctcgaacacg ttctcggtcg cgatgctcgt cccgttcgcc
     4441 gtggagagca cggccagcag gatcggctgg aaatcggtcg cgatccccgg gtacgggagc
     4501 gtcacgaagt cggcggcccg cgcccgttcc ggctgccgga cgcgcagccc gtcgtcggtc
     4561 ctgcggatct cggcgccacc ctcgccgagc ttctcgagga agagctccag gtgctcgggg
     4621 cgcgccccgt cgagcacgag atccccgcgg gtcgccaccg cggccgcggc ccaggttccc
     4681 gcctcgatcc gatcgcagat gaccgtgtgc tcggccggct cgagcgcgcc caccccttcg
     4741 atctcgatcg tcgcggaccc ggcgccgtgg atcccggccc ccatcgccgc gaggaacgcg
     4801 gccaggtccg cgatctcggg ctcccgcgcg gcgttgtcga tcacgctgac gccgtcggcg
     4861 atgaccgccg ccatcagcag gttctcggtg gcccccacgc tcggatagtc gagggtgatc
     4921 tgagcggccg tgagtccgtc ggcggtcgcc acgaggaacc cgtgctccgc gtcgatccgg
     4981 gcgcccatcc ggcgcagtcc atcgagatgg agatcgatcg gacgtgaacc gatgttgtcg
     5041 ccgcccggca tcgctacgcg cgcttcaccg gtccgggcga gcagcgggcc gagcacggcg
     5101 atcgaggccc gcatgcgccg gaccagctcg tacggcgcct ccacggatga gaggtgggtc
     5161 gcatccaccg cgacgcctcc gtcctcccac gagacccggg cgccgagttg ctcgaggacc
     5221 tcgcccatcg tgaagcagtc ctggatgcga ggaacgttgc ggagcgcgga acgtccgggt
     5281 gcgagcagcg acgccgccat cagcttcagg gccgagttct tggcgcccga gatgcggacg
     5341 gaaccagtga ggcgagcgcc ccccgtcacg aagagacggt ccatctgcgc atcgtaccgg
     5401 ggcccgcctg cgagacggcc gcgacggcga cgatcagtcg gagccgatca gggtgagcct
     5461 ggcttcggcc cgcgcgatct cggtcttgac ctgctcgtcg tcctcacctt cggcggagga
     5521 gcgcagccct tcgagacgcc tcctgacggc gtcgacgtcg atctcggcct cgagctgggc
     5581 gttcgttgcg agcacgtcca cccgggatgc gccctcctcc gtggacacgt gcaggaaacc
     5641 gccgtccacc accgccttga gccaggtgcc gtcctcctcc tggatctgga gcggtccgat
     5701 cgccaagtgg acgaggagcg gagcatgtcc ggccaagatg ccgacgtcgc cgtcgacccc
     5761 gtgggcgatc acctccgtgg cctgccccga ccacacctcg cgctcgggcg tgacgacgtg
     5821 gacctcgagc gccactacac agcctcgagg gtcttcgcct gctcgacggc ctcctcgatc
     5881 ccgccgacga ggaagaacgc ctgctcgggg aggtggtcgt actcgccttc gcagagcgcc
     5941 ttgaacgacg cgatcgtctc gtcgatcg
//
LOCUS       DASUVE010000180         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_470354, whole genome shotgun sequence.
ACCESSION   DASUVE010000180 DASUVE010000000
VERSION     DASUVE010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470354"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VF036_03840"
     CDS             <1..250
                     /locus_tag="VF036_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEX6264395.1"
                     /translation="DLSAYNILIWDEDVTVIDFPQAVDPKLNRHAQAFLQRDVRRLGE
                     FFEHHGVRHPWERIADDLWNGWLFADLVPEDLRGELLL"
     gene            288..851
                     /locus_tag="VF036_03845"
     CDS             288..851
                     /locus_tag="VF036_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6264396.1"
                     /translation="MRLSGTARSRIHAGMQTEGDVVRLQAEGQSWASFDAFFEAEHER
                     LFKALYFVTGNLHDAEELMQDAFLTMWERWGDLGRIDDVTGYLFRVALNGSRMRWRRA
                     EMAKRKLALVGRPVDPFADAEVRVDVQAMLQRLTPRQRAALLLTELMGYPSDEAASIM
                     GVRASTVRALATQGRQAMRATRGDDDA"
     gene            844..1977
                     /locus_tag="VF036_03850"
     CDS             844..1977
                     /locus_tag="VF036_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264397.1"
                     /translation="MPDLRDAFRIATDHAVPEPGALERQLRHQRRRSARRKAGAFVAV
                     AAVLALIAGVILATRPGTGDDDDRFADPIAPSFPLFTLDIGSGEQAPFSELGILLEHF
                     ELSPDGTTLMASQSVKWNAGERRLWTADADGSHISLLMDRDATIGPWFPDETRIAFVG
                     LPLPDDDGVRTLENADTRQVIVLDRSTGQTTVLTDEETDPSDPTVTPDGSTVVYAVSE
                     RLDAGAAVGAPDGDEPGAYISDVPPTSWLRAVDVDTRQTRTLATAPRLHLWEPDVSPS
                     GELTWITGRFDYLGFADRSSLHSMRLGGGGERTIISSQTDDSVVWLDTPMWSPDGSLL
                     AYYRDNPEGNSAIWLYDPTTGEHREVEHGWLETWLDGDTLLIS"
BASE COUNT          327 a          737 c          714 g          303 t
ORIGIN      
        1 cgacctgtcc gcctacaaca tcctgatctg ggacgaggac gtcacggtga tcgacttccc
       61 gcaggccgtg gaccccaagc tgaaccggca cgcgcaggcc ttcctccagc gcgatgtccg
      121 acggctcggt gagttcttcg agcatcacgg cgtccgccac ccctgggagc ggatcgccga
      181 cgacctgtgg aacggctggc tgttcgccga cctcgtcccc gaggatctgc gcggggagct
      241 gctcctgtag cgcagcgcat ccggcccgca ggtgcgagga tcctgccatg cgcctcagcg
      301 gaacggcccg ttcacgcatc catgccggga tgcagaccga gggcgatgtc gtgcggctgc
      361 aggccgaggg gcagtcctgg gccagcttcg atgccttctt cgaggccgag cacgagcgcc
      421 tgttcaaggc cctgtacttc gtgaccggca acctccacga cgccgaggag ctgatgcagg
      481 acgccttcct cacgatgtgg gagcgctggg gtgatctggg gaggatcgac gacgtgacgg
      541 gctacctgtt ccgggtggcg ttgaacgggt ccaggatgcg ctggcggcgg gccgagatgg
      601 cgaagcgcaa gctcgcgctc gtcggccggc ccgtggatcc gttcgccgac gccgaggtcc
      661 gcgtcgacgt gcaggcgatg ctgcaacggt tgacgccgcg ccagcgcgca gccctgctgc
      721 tcaccgagct gatgggctat ccgtccgacg aggcggcgtc gatcatgggc gtccgggcat
      781 cgacggtccg ggcgctcgcc acgcagggcc gccaggccat gcgcgccacc cgaggagacg
      841 acgatgcctg acctgcgtga cgccttccgc atcgcgaccg accatgccgt gcccgagccc
      901 ggcgcgctcg aacgtcagct ccggcaccag cggcgtcgat cggcacgacg gaaggcgggc
      961 gcgttcgtcg cggtcgcggc cgtcctcgcg ctgatcgccg gcgtgatcct ggcgacgcgc
     1021 ccgggtaccg gagacgacga cgacaggttc gcggacccca tcgcccccag cttcccgttg
     1081 ttcacgctcg acatcggaag cggcgagcaa gccccattct ccgaactcgg catcctgttg
     1141 gagcacttcg aactgtcccc cgacgggacg acgctcatgg catcgcagag cgtcaagtgg
     1201 aacgccgggg aacgtcggct gtggacggcg gacgccgacg ggtcgcacat ctccctgctg
     1261 atggaccggg acgccacgat aggaccgtgg ttccccgacg agacgcgtat cgccttcgtc
     1321 gggctgcccc tccccgacga cgacggcgtg cgcaccctcg agaatgccga cacccgacag
     1381 gtcatcgtcc tcgaccggtc gaccggacag acgaccgtcc tcaccgacga ggagaccgat
     1441 ccctccgatc cgaccgtgac acccgacggg tcgacggtgg tgtacgcggt gagcgagcgg
     1501 ctggacgcgg gtgcggccgt cggcgctccc gacggtgacg agcccggtgc ctacatctcc
     1561 gacgtgccac cgacgagctg gctccgggcc gtcgacgtcg acacgcggca gacgcggacg
     1621 ctcgcgaccg caccgcggct ccacctctgg gagccggacg tgagcccctc cggcgagctc
     1681 acctggatca cggggcggtt cgactacctc gggttcgccg accggtcctc gttgcactcc
     1741 atgcggctcg gcgggggcgg ggagcggacc atcatctcgt cgcagaccga cgacagcgtc
     1801 gtgtggctcg acacgccgat gtggtcaccc gacggatcgc tcctcgccta ctaccgcgac
     1861 aaccccgagg ggaactcggc gatctggctc tacgacccga cgaccggaga gcatcgagag
     1921 gtggaacacg gatggctcga gacgtggctc gacggggaca ccctcctgat cagctgaccg
     1981 ggtctgaggg cggggccgct gcggagccgg gccccgccct cacactccag acctgggcac
     2041 cccggagcct ctgcagcgca tcgcgggaag gatcatgctg t
//
LOCUS       DASUVE010000181         4243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_92446, whole genome shotgun sequence.
ACCESSION   DASUVE010000181 DASUVE010000000
VERSION     DASUVE010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92446"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..2552
                     /locus_tag="VF036_03855"
     CDS             252..2552
                     /locus_tag="VF036_03855"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020517890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX6264398.1"
                     /translation="MATSKSKQIGSKDLLLESGTFAKLADGAAVVRYGDTMVLATCVS
                     SAPREGIDFFPLTIDVEERMYARGKIPGSFFRREGRPSETAILTARLIDRPLRPSFRE
                     GYRDEVQVVVTVLSVDMENQYDIPAMNAASLAVGIAGLPFDGPVGSVRLGLIGADWVV
                     NPSFQELDEATFDIVVAGSKNEQGGIDILMIEGEAPDETWPLLAAGEGATAPTEEVVA
                     QGLERAKEVITEIIGLQEEFLAEVGVKPSDFEPHPLYQQDLWDALESFGKDKVLTALV
                     SDRKEREANMSAVKDEAKAHLAQTLGEDVFAARASEFSPAWKSLEKKVMRARVISDGI
                     RLDGRGPKDIRPLAAHVGLLPRAHGSSLFERGDTQVLNVTSLGMLRMNQMIDTLDPED
                     SKRYIHHYNFPPYSTGETGRVGSPRRREIGHGALAERALIPVVPSEEEFPYTLRLVSD
                     VLSSNGSTSMASVCASTLSLMDAGVPIKAPVGGIAMGMIAEGDTFVTLTDILGAEDAL
                     GDMDFKVAGTREFVTAIQLDMKVSGMPADVLAGALQQAREARFTILDVMEAAIPSPRD
                     AVNPIAPRIITITIPVDKIGEVIGPKGKRINEIIAQTGADIDIQDDGTVFIGSHEGEG
                     ADEAARMIDEIANPRPVLQGETYDGTVVKTTTFGAFVNLVPGRDGLVHISKLGKGKRL
                     SSVEEAVREGDTLTVLVEDIDAQGKISLKPTGPEWEAPEGEGSSGGERREPREPREPR
                     EPRNGADRGPRRTGRRFRDADKGSSA"
     gene            2577..3851
                     /locus_tag="VF036_03860"
     CDS             2577..3851
                     /locus_tag="VF036_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015624861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX6264399.1"
                     /translation="MGIERTEFSSGLRVVTERMPGVRSVSLGFWVLAGSRDEPPAISG
                     SSHFLEHLLFKGTKTRSAREIAEDFDAVGGDVNAFTSKEYTCYYARILDGDLEMAVDH
                     LSDMFQGSVLRSADLDAERQVILEEINMHEDSPEEVVHDLFTEALWPNHPLGRPVLGA
                     AETIRAATRDKVHRFYRKHYVPGGLVVAAAGNLRHQELLDMLRRRMDTGRIVRSGDPQ
                     GWNLRASETAPESSAKPLVKRRKTEQAHICLGTNGLARSDPDRFPFLIVNGALGTGMS
                     SRLFQEIREKRGLVYSVYSYHSQYTEAGLFSCYAGTTPARAQEVIGLLRRELDDVAEG
                     GLTAEEFDRAKGHVKGSMVLSLEDPGSRMSRIGKSEIAHGEILTVNQSLKRVEDVTLE
                     DARRVAKRVLSQPMTLTVLGPFAQRDLAGVMS"
     gene            3848..>4243
                     /locus_tag="VF036_03865"
     CDS             3848..>4243
                     /locus_tag="VF036_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX6264400.1"
                     /translation="MIRVGVIGATGKVGAEVCRAVHADADLELVAGISRSKAGEPVST
                     ALGLGGSDVVLAENLDAFDAARVEVAVDFTSGSFAPEHVAWAIEHGVHIVVGTTGFDV
                     DEAWREAPVGVFVAPNFAIGAALMQRFARE"
BASE COUNT          709 a         1428 c         1495 g          611 t
ORIGIN      
        1 cgccatcatc cgcagcagcg cgccgggggc gcaagaagag gagtgatgcc cacgacccac
       61 tgacccgacc ggaggcggag gccccacaca ggtcggttct cagtcgcgga cccccgggac
      121 cgcgcgcagc gcccaggatc cgcgcgcgcg ccgaactcga aaccggaggc ccaccactga
      181 caaccggccc agccgcctcc cgcagctgcg gcgccccgag gtgcccgccc acaacaggag
      241 gtacgaggcc tatggccacc agcaagtcga agcagatcgg gagcaaggat ctgctcctcg
      301 agtccggtac gttcgcgaag ctcgccgacg gcgcggccgt cgttcgctac ggcgacacga
      361 tggtgctcgc cacgtgtgtg agctccgcac cccgagaggg gatcgatttc ttcccgctca
      421 cgatcgacgt cgaagagcgg atgtacgccc gggggaagat ccccggcagc ttcttccgtc
      481 gagagggccg tccgtcggag acggcgatcc tcaccgcgcg gctgatcgac cgtccgctac
      541 ggccctcgtt ccgcgagggc taccgcgacg aggtgcaggt cgtcgtcacg gtcctctcgg
      601 tcgacatgga gaaccagtac gacatcccgg cgatgaacgc cgcgtcgctc gcggtcggga
      661 tcgcgggcct gccgttcgac ggtcccgtcg ggtccgtccg tctcgggctg atcggcgcgg
      721 actgggtcgt gaacccctcg ttccaggagc tcgacgaggc cacgttcgac atcgtggtcg
      781 caggctcgaa gaacgagcag gggggcatcg acatcctgat gatcgaggga gaggcgcccg
      841 acgagacctg gccgctcctc gccgccggcg aaggcgccac cgcgcccacc gaggaggtcg
      901 tcgcgcaagg cctcgagcgg gcgaaggagg tcatcaccga gatcatcggt ctccaggagg
      961 agttcctggc cgaggtcggc gtgaagcctt cggacttcga gccgcacccg ctctatcagc
     1021 aggacctctg ggacgcgctc gagtcgttcg ggaaggacaa ggtgctcacc gccctcgtct
     1081 ccgatcggaa ggagcgcgag gcgaacatgt ccgccgtcaa ggacgaggcg aaggcccacc
     1141 tcgcccagac gctgggagag gacgtcttcg ccgctcgcgc gagcgagttc tcgccggcgt
     1201 ggaagtcgct cgagaagaag gtcatgcggg cccgggtgat ctcggacggc atccggctcg
     1261 acggtcgcgg cccgaaggac atccggccgc tcgcggccca cgtgggactc ctgccccgcg
     1321 cacacgggtc gtcgctgttc gagcgcggtg acacgcaggt gctcaacgtc acgtcgctcg
     1381 ggatgctccg gatgaaccag atgatcgaca ccctcgatcc cgaggactcc aagcggtaca
     1441 tccaccacta caacttcccg ccgtactcca ccggggagac gggccgcgtg ggctcgcctc
     1501 gccgccggga gatcggtcac ggagcgctcg ccgagcgggc gctgatcccc gtggtcccct
     1561 cagaggagga gttcccgtac acgctgcgac tggtctccga cgtgctttcg tcgaacgggt
     1621 cgacatcgat ggcaagcgtg tgcgcctcga cgctctcgct gatggatgcg ggcgtgccga
     1681 tcaaggcccc cgtcggcggg atcgcgatgg ggatgatcgc cgaaggcgac acgttcgtga
     1741 cgctgacgga catcctcggc gcggaggatg cgctgggcga catggacttc aaggtcgccg
     1801 gcacgcggga gttcgtcacc gcgatccagc tcgacatgaa ggtcagcggg atgcccgccg
     1861 acgtgctcgc gggtgcgttg cagcaggccc gcgaggctcg cttcacgatc ctcgacgtga
     1921 tggaggcggc gatcccctct ccgcgcgacg ccgtgaaccc gatcgcgccg cggatcatca
     1981 cgatcacgat cccggtcgac aagatcggcg aggtgatcgg tcccaagggc aagcggatca
     2041 acgagatcat cgcccagacc ggcgccgaca tcgacatcca ggacgacggc accgtgttca
     2101 tcggttccca cgaaggtgag ggggcggacg aggcggcccg gatgatcgac gagatcgcga
     2161 acccgcgtcc cgtccttcag ggcgagacct acgacggcac cgtcgtgaag acgacgacgt
     2221 tcggggcgtt cgtgaacctc gtgcccggca gagacggtct cgtgcacatc tcgaagctgg
     2281 gcaaggggaa gcgcctcagc tccgtggagg aggcggtccg ggagggcgac acgctcacgg
     2341 tcctcgtaga ggacatcgac gcccagggca agatcagcct caagccgacc ggtcctgagt
     2401 gggaggcgcc ggagggcgag ggttcctcgg gcggtgagcg ccgagagccg cgagagcccc
     2461 gcgagccgcg cgagccccgc aacggagccg accgtgggcc gcgtcgcacc ggacgccggt
     2521 tccgcgacgc cgacaagggg tcctcggcct aaggccgaac gatccggagg cggacagtgg
     2581 ggatcgagcg aaccgagttc tcctcggggc tgcgagtcgt cacggagcgg atgcccggcg
     2641 tccgctccgt gtcgctcggc ttctgggtgc tcgccggatc ccgcgacgag cctccggcga
     2701 tcagcggctc gagccacttc ctcgagcacc tgctcttcaa gggaacgaag acgaggtccg
     2761 cccgcgagat cgcggaggac ttcgatgccg tcggcggcga cgtcaacgcg ttcacgtcga
     2821 aggagtacac ctgctactac gcacgcatcc tcgacggcga cctggagatg gcggtcgatc
     2881 acctgtccga catgttccag ggctcggtgc tccggtcggc cgatctcgat gcggagcgtc
     2941 aggtgatcct cgaggagatc aacatgcacg aggactcgcc cgaggaggtc gtccacgacc
     3001 tcttcaccga agccctctgg ccgaaccatc cgctggggcg gcccgtcctc ggcgccgccg
     3061 agacgatccg cgcggcgacg cgggacaagg tccaccggtt ctaccgcaag cactacgtgc
     3121 cgggaggact cgtcgtggcg gccgcgggga acctccgtca ccaggagctg ctcgacatgc
     3181 tccgccgtcg catggacacg ggacggatcg tgcggtcggg ggacccgcag ggatggaacc
     3241 tccgcgccag cgagaccgcc cccgagtcat ccgcgaagcc gttggtgaag cggcggaaga
     3301 ccgagcaagc gcacatctgt ctgggcacga acgggctcgc gcggagcgac cccgaccggt
     3361 tccccttcct gatcgtgaac ggcgcgctgg gaaccgggat gtcctcgcgc ctgttccagg
     3421 agatccgtga gaagcgcggc ctcgtctatt ccgtgtacag ctatcactcg cagtacaccg
     3481 aggcggggct gttctcctgc tacgcgggga ccaccccggc ccgcgcgcag gaggtgatcg
     3541 gcctgctccg tcgcgagctg gacgacgtcg cggaaggcgg tctcacggcc gaggagttcg
     3601 atcgcgcgaa gggacacgtg aaagggtcga tggtgctctc gctcgaggat cccgggagcc
     3661 ggatgtcgag gatcggcaag tccgagatcg cgcacggcga gatcctcacg gtgaaccagt
     3721 cgctgaaacg cgtcgaggac gtgacgctcg aggacgcacg acgggtggcc aagcgggtgc
     3781 tgtcgcaacc gatgaccctc accgtcctcg ggccgttcgc gcagcgcgat ctcgcggggg
     3841 tgatgtcgtg atccgcgtcg gcgtgatcgg tgccaccggc aaggtgggcg ccgaggtgtg
     3901 tcgcgccgtg cacgccgacg cggacctcga gctcgtcgcc gggatctccc gctcgaaggc
     3961 gggcgagccg gtctccacgg ccctcgggct ggggggctcc gacgtcgtcc tggcggagaa
     4021 cctcgacgcg ttcgacgcgg cgagggtcga ggtcgcggtc gacttcacga gtggcagctt
     4081 cgcgcccgag cacgtcgcct gggcgatcga gcacggcgtg cacatcgtcg tcgggaccac
     4141 gggcttcgac gtggacgagg cgtggcgcga agcgccggtc ggtgtcttcg tcgctccgaa
     4201 cttcgcgatc ggcgccgcct tgatgcagcg gttcgctcgc gag
//
LOCUS       DASUVE010000182         3774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_658072, whole genome shotgun sequence.
ACCESSION   DASUVE010000182 DASUVE010000000
VERSION     DASUVE010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658072"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VF036_03870"
     CDS             complement(<1..122)
                     /locus_tag="VF036_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017565964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6264401.1"
                     /translation="MRIESVVFDCHDAAPLARFWADALGWSVAPYDEEELERLAS"
     gene            complement(173..805)
                     /locus_tag="VF036_03875"
     CDS             complement(173..805)
                     /locus_tag="VF036_03875"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEX6264402.1"
                     /translation="MGTRDDAIGSWLDELGSASPTPGGGAAAGVAAAMGAALIAMVGH
                     LTVGKEGFEDLDARMRTLVETAGDEGKAFLALADEDAAAFESVIAAFRLPKGTDEEKA
                     SRTMRIQEAYAGAAAVPLQLATRCVRLMDLAEDATAMGNPHASSDGYSAGLLLFAAAL
                     AAMANVRINAAGLKDEATGQGLVDECDALRARADALLQQLEEAFLLRLSA"
     gene            complement(836..1732)
                     /gene="ftcD"
                     /locus_tag="VF036_03880"
     CDS             complement(836..1732)
                     /gene="ftcD"
                     /locus_tag="VF036_03880"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEX6264403.1"
                     /translation="MDAIVGVVPNFSEGRRGDVIDAIVDALDVDGARVVFAEADADHH
                     RLDTTVLGSAEGVARAAVSGAAVAIDRIDLFEHHGGHPRMGAVDVVPFMPVRGVEMEE
                     CVDLARSVGRELAESFSLPVYLYGEAAVVPHRRSLADVRKGEFEGLRAAVAAGDRLPD
                     YGPHELGRAGATAVGARKPLVAFNTYLDGTDEGAAKEIAREVRESGGGLPAVRAIGFA
                     VPERGCVTVSMNLVDFEVTGVGAAFDAVVGAAAARGLEVLDSEIVGLTPVAALPEGTS
                     EHVRLAGFSEETQILERLVETG"
     gene            complement(1761..2861)
                     /locus_tag="VF036_03885"
     CDS             complement(1761..2861)
                     /locus_tag="VF036_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEX6264404.1"
                     /translation="MDETSRKTAFHEIMAKTAAGFMEEAGWFWVTGFGDPEAEYEAVR
                     NGVGVWDVSPLNKWDFRGPDAVEAAQRVHSNDIMGMQTGQVRYGAFLDEAGLLVDDGT
                     VFKHADDHLWVCTNGLDHEAYFADATKGLDVAVDYIAPDLPHLQVQGPRSRDALQPIT
                     DADLGSIGYFRFLPDLVAVGGVPVWLSRTGFSGELGYELFTKPEHAEDLWTAIEGIGA
                     TPYGVDVVEPIRIEAGMIVTDYDYEAHQRTPYDLGLDRLVALDGEGSFMGKEALREVA
                     GAPPNRFKTLRLEGQELPEYGATVTKDGAEVGVLTSPASSPTFGPIGLAILRSNVAAD
                     GTAVDVALGDGTIGATVDVLAVVDPEKRRPRG"
     gene            complement(2874..3470)
                     /locus_tag="VF036_03890"
     CDS             complement(2874..3470)
                     /locus_tag="VF036_03890"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX6264405.1"
                     /translation="MNSGALKRAKRQIRRAVLEERDALSPGVRAERGERIARRVVELP
                     EVRDVRAVMLFSSFGSEVPTAPLIERLRQRGIVVALPRIEAGELVPVAYTPGDPVRTA
                     SFGALEPIGPNGLEPASLDVVGVPGVAFDRFGGRVGYGGGYYDRFLRGLPAFRVGIAF
                     GLQVVEGPLPAGRFDLSVQAIVTEEETIRPDLNSRSIS"
     gene            complement(3467..>3774)
                     /locus_tag="VF036_03895"
     CDS             complement(3467..>3774)
                     /locus_tag="VF036_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264406.1"
                     /translation="GWGMDADRFAVAVHELRRVADGRTVTPTWGGIALVGRDAADLER
                     LRVERSAKGLSMDVWQGDGPAFRAFAERLREMDCSWIVVLPVGGADRIEVVAEALGR"
BASE COUNT          584 a         1432 c         1225 g          533 t
ORIGIN      
        1 gaggcgaggc gttcgagctc ctcctcgtcg tacggggcga cggaccatcc cagcgcatcc
       61 gcccagaacc gggcgagcgg cgcggcatcg tggcaatcga agacgaccga ttcgatcctc
      121 accgtcctcg aagcctaggc cacgcagcag gtgggcgctc gggtcgcagc cgtcaggcgg
      181 acagacgcag gaggaatgct tcctcgagct gctggaggag ggcgtcggcg cgcgcgcgca
      241 acgcgtcaca ctcgtcgacg agcccttgac cggtcgcctc gtccttgaga ccggcggcgt
      301 tgatccggac gttcgccatc gcggcgaggg cggccgcgaa caggaggagt cccgccgagt
      361 agccgtcgga ggacgcatgg ggattcccca tcgcggtcgc gtcctccgcc aggtccatca
      421 agcggacgca acgcgtggcg agctggagcg ggaccgcggc ggcgcctgcg tacgcctcct
      481 ggatccgcat cgtccgcgag gccttctcct catccgtccc cttggggagt cggaaggccg
      541 ctatcaccga ctcgaacgcg gcggcgtcct cgtcggcgag cgccaggaag gccttcccct
      601 catccccggc cgtctccacc agggtgcgca tgcgcgcgtc cagatcctcg aacccctcct
      661 tgccgacggt gagatgtccc accatcgcga tcagggccgc ccccatcgcg gcggcgaccc
      721 cggcggcggc accgcctccg ggggtcggcg atgccgagcc gagctcgtcc agccacgatc
      781 cgatcgcgtc gtcgcgcgtg cccataggtc tcctctcttc ccacgagacc ggccctcagc
      841 cggtttcgac gagccgttcc aggatctgcg tctcctccga gaagccggcg agccgtacgt
      901 gttccgacgt cccctccggc agggccgcca ccggcgtgag tccgacgatc tcggaatcca
      961 gcacctccag gccgcgcgca gccgccgcac ccacgacggc atcgaacgcc gccccgacgc
     1021 ccgtcacctc gaagtccacg aggttcatgg acacggtgac gcaccctcgt tccggcaccg
     1081 cgaagccgat cgcccgaacc gcggggaggc cgcctccgga ctcgcggacc tcgcgggcga
     1141 tctccttcgc cgccccctca tcagtgccat ctaggtaggt gttgaacgcg accaacggtt
     1201 tccgtgcccc cacggctgtc gcgcccgcgc gcccgagctc gtgcggaccg tagtcgggca
     1261 gccgatcgcc cgccgccacg gccgcgcgga gcccctcgaa ctcgcccttc cgcacatcgg
     1321 cgagcgagcg gcgatggggc acgaccgcgg cctcgccgta gaggtacacg gggagggaga
     1381 acgactcggc cagctcccgc ccgaccgagc gagcgagatc gacgcattcc tccatctcga
     1441 ccccgcgaac gggcatgaac ggcacgacgt cgacggcgcc catccgtggg tggccgccgt
     1501 ggtgttcgaa caggtcgatc cgatcgatcg ccacggcggc tcccgagacg gcggcccgcg
     1561 cgacgccctc ggccgacccg aggacggtcg tgtcgaggcg atgatgatcc gcgtcggcct
     1621 cggcgaacac gacgcgtgcg ccgtccacgt cgagcgcgtc gacgatcgca tcgatcacgt
     1681 cgccccgccg tccctcgctg aaattcggca ccacgccgac gatcgcatcc atgccggcga
     1741 gcctacggga gcggcgggga tcagcccctg ggccggcgct tctcgggatc gacgacagcg
     1801 agcacgtcga ccgtcgcgcc gatcgtgccg tcaccgagtg cgacgtcgac cgccgtcccg
     1861 tccgccgcga cgttggaccg gaggatcgcg agaccgatcg gaccgaacgt gggactgctc
     1921 gccggcgagg tcagcacgcc gacctccgca ccgtccttcg tgacggtcgc accgtactcg
     1981 gggagctcct gcccctcgag ccggagcgtc ttgaaccggt tcggtggcgc tcccgcgacc
     2041 tcccgcagcg cctccttccc catgaaggaa ccctcgccgt ccagggcgac gagccggtcc
     2101 aacccgaggt cgtacggcgt ccgctgatgg gcctcgtagt cataatcggt cacgatcatc
     2161 ccggcctcga tccggatcgg ttcgacgacg tccaccccgt agggggtcgc gccgatgccc
     2221 tcgatcgccg tccacaggtc ctcggcatgc tcgggcttcg tgaacagctc gtagccgagc
     2281 tcacccgaga agccggtgcg cgagagccag acggggacgc cgccgacggc cacgagatct
     2341 ggcaggaacc ggaagtaccc gatgctcccc aggtccgcgt ccgtgatcgg ctgcagggcg
     2401 tcccgcgagc ggggaccctg gacctggagg tgtggcaggt cgggggcgat gtagtcgacg
     2461 gcgacgtcga gccccttcgt ggcatcggcg aagtacgcct cgtggtccaa cccgttcgtg
     2521 cagacccaga gatggtcgtc cgcgtgcttg aacaccgtgc cgtcgtcgac cagcaggccg
     2581 gcctcgtcca ggaacgcccc gtagcgcacc tgtccggtct gcatccccat gatgtcgttg
     2641 gagtggaccc gctgcgcggc ctcgacggcg tcgggacctc ggaagtccca cttgttgagc
     2701 ggggagacgt cccagacgcc caccccgttc cgaacggctt cgtattcggc ttccgggtcg
     2761 ccgaagcccg tcacccagaa ccagccggcc tcttccatga acccggctgc cgtcttcgcc
     2821 atgatctcgt ggaacgccgt cttcctgctc gtctcatcca tgcgcccctc ttatcacgag
     2881 attgaacggc tgttcaagtc cggtcgaatc gtctcctcct ccgtcacgat ggcctggacg
     2941 gagagatcga acctcccggc cgggagcggg ccctcgacca cctgcaggcc gaacgcgatg
     3001 ccgacccgga acgcggggag gcctcggagg aaccggtcgt agtagccgcc cccgtacccg
     3061 acccggcccc cgaaccgatc gaacgcgacg ccgggaaccc ccaccacgtc gagcgatgcc
     3121 ggctcgagac cgttcggacc gatcggctcg agggcgccga aggacgccgt gcgcacggga
     3181 tcgccgggcg tgtacgcgac cggtacgagc tcccccgcct cgatccgcgg aagcgccacg
     3241 acgatccctc gctggcggag ccgttcgatc aacggcgccg tcggaacctc ggatccgaac
     3301 gacgagaaca gcatcaccgc tcgaacgtcg cggacctcgg gaagctccac gacgcgccgg
     3361 gcgatccgct ccccccgctc ggcgcgcacg cccggcgaca gggcgtcgcg ctcctcgagc
     3421 acggctcgcc ggatctgccg tttcgcgcgc ttcaacgccc cgctgttcat cgcccgagag
     3481 cctcggccac gacctcgatc cgatcggcgc cgccgacggg aagcacgacg atccacgagc
     3541 agtccatctc ccgcagccgc tcggcgaacg cacggaaggc cgggccatca ccttgccaga
     3601 catccatgga caggcctttg gcgctgcgct cgacgcggag gcgttcgagg tcggcggcat
     3661 ccctgccgac gagggcgatc ccaccccacg tcggagtcac cgttcggccg tccgccacgc
     3721 ggcggagctc gtgcacggcc acggcgaatc gatcggcgtc catcccccag ccgt
//
LOCUS       DASUVE010000183         3221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_93129, whole genome shotgun sequence.
ACCESSION   DASUVE010000183 DASUVE010000000
VERSION     DASUVE010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93129"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..234
                     /locus_tag="VF036_03900"
     CDS             49..234
                     /locus_tag="VF036_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264407.1"
                     /translation="MALRSPNEIPGRPSGVLVPTYSDNEHPGTKAERRRLAVEWRRRH
                     PQWWKRLLRLKSTPPAD"
     gene            complement(214..735)
                     /locus_tag="VF036_03905"
     CDS             complement(214..735)
                     /locus_tag="VF036_03905"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HEX6264408.1"
                     /translation="MEPAFSKEGDRFTVRLSRGVRGLLVELCQQSRALLDAEDPSSDP
                     AVSRLFPPAYPDDPLQNLEFETSLGAAPRSGKLEALDTVERTANASTLTEDELLAWIG
                     VVNDLRLVLGTRIEITEEATDEDFPADDPRHETYRVYQFLGFLLHELLEGIGAPDVGE
                     PPNEGEGSVGGGS"
     gene            complement(735..1040)
                     /gene="clpS"
                     /locus_tag="VF036_03910"
     CDS             complement(735..1040)
                     /gene="clpS"
                     /locus_tag="VF036_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX6264409.1"
                     /translation="MWLRTTTTRPGRVQRPHVDEVNEPDLPWIVIVWNDPINLMGYVT
                     WVFMRLFGYPREKAERLMWDVHTKGRAVVSSGPREKAESDVARLHAHGLWATLQRDA"
     gene            complement(1040..1801)
                     /locus_tag="VF036_03915"
     CDS             complement(1040..1801)
                     /locus_tag="VF036_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6264410.1"
                     /translation="MVAGASSGIGEAAALAFAEHGARVVLAARRTERLDAIVERIERK
                     GGKALAATCDVTEPEQLTTLRVVTEEVYGPADVLVNSAGMRGGGEFTKLDYDRIEDHV
                     RLNILGVLFGTRAFLPSMLARGKGHVVNVGSLAGRFATPGNPIYGATKHAVVAFSEAM
                     NYETEGRNVHVTSVNPGFVDTAGFPQGDLPDWVVLKMRTVTDAIVKVVRDDIAPEYSV
                     PRWLSPFQLFRVVTPPLYRWGIRRVRRARRSLPSR"
     gene            1855..2688
                     /locus_tag="VF036_03920"
     CDS             1855..2688
                     /locus_tag="VF036_03920"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEX6264411.1"
                     /translation="MDVIGAHAGRLPVLDERWEPRPYPREELERALVAGGVAGPAGHP
                     HHNVFGNIRKLVEHDPDKLFGLSSMPEPFGFDDIVRIVGEASGVPIDALATEGSPDID
                     AASVVDACERVGERLAGAARRRERVLFATGHPGDLDPLYGAIAELAAARGAEIVRPAD
                     GEAWFDDHAGKEWTIAYHGAVGMVTDGERPRHTHWPEAMRRMLADERPDLVMADHGLA
                     GAAIEAGVETLSIADVNDPALIVAKAQGRTEIVVVLDDHVPPENYWPCFQAVAGRFDP
                     S"
     gene            complement(2685..2987)
                     /locus_tag="VF036_03925"
     CDS             complement(2685..2987)
                     /locus_tag="VF036_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264412.1"
                     /translation="MTDARRALLRKLEHASAFPMVALQAIRELRTELDAIEAEALLRA
                     RELGASLEDIAEALEITRQGVSYRLKALADAGHDDGPADDEIVDVREPDPRGARSD"
BASE COUNT          498 a         1106 c         1124 g          493 t
ORIGIN      
        1 aagcgggcgg ttgtgccggg ttcggaggac gcctagcgtg aacgggcgat ggccctccgc
       61 tctccgaacg agatccctgg ccgcccctcc ggcgtgctcg tcccgaccta cagcgacaac
      121 gagcatccgg ggacgaaggc ggagcggcgg cgcctcgcgg tcgagtggcg acggcggcac
      181 ccgcagtggt ggaagcggct gctccgcctg aagtcaactc cccccgccga ctgagccctc
      241 tccctcgttg ggcggctcgc ccacgtcggg cgcgccgatc ccctcgagca gctcgtggag
      301 caggaagccg aggaactggt acacgcggta ggtctcgtgc cgaggatcgt ccgcggggaa
      361 gtcctcgtcc gtcgcttcct cggtgatctc gatccgcgtg ccgagcacca gtcgcagatc
      421 gttcaccacg ccgatccacg cgaggagctc gtcctcggtc agcgtcgacg cgttcgcggt
      481 gcgctccacg gtgtcgaggg cctccagctt ccccgaccgc ggagcggcgc cgaggctcgt
      541 ctcgaactcg aggttctgca gcgggtcgtc cgggtacgcg ggcgggaaga gccgggacac
      601 cgccgggtca ctcgacgggt cctccgcgtc gagcagcgcg cgggactgtt ggcagagctc
      661 gacgaggagg ccgcgcacac cccggctgag gcgcacggtg aacctgtcgc cctccttcga
      721 gaacgcgggc tccatcacgc gtcccgctgc agcgtcgccc agagcccgtg cgcatggagg
      781 cgggcgacgt cggactccgc cttctcccgg ggaccggagg agacgacggc gcgtcccttg
      841 gtgtgcacgt cccacatcag gcgctcggcc ttctcgcgcg gatacccgaa gaggcgcatg
      901 aacacccacg tcacgtagcc cataaggttg atggggtcgt tccagacgat cacgatccac
      961 ggcaggtcgg gctcgttgac ctcgtcgacg tgcgggcgct ggacgcgacc cggtctggtc
     1021 gtggtcgtcc ggagccacat catcggctcg gcagggatcg acgggcccga cgcaccctcc
     1081 ggatccccca tcggtagagc ggtggcgtga cgacgcggaa gagctggaac gggctcagcc
     1141 atcgagggac ggagtactcg ggcgcgatgt cgtcgcggac caccttcacg atcgcgtccg
     1201 tcacggtccg catcttgagc accacccagt ccggaaggtc gccctgcggg aatcccgccg
     1261 tgtcgacgaa ccccgggttg accgacgtca cgtgcacgtt gcgtccctcg gtctcgtagt
     1321 tcatcgcctc ggagaacgcg acgacggcgt gcttcgtcgc accatagatc gggttgccgg
     1381 gcgtcgcgaa gcgtcctgcc aacgacccca cgttgacgac gtggcccttg ccccgagcga
     1441 gcatgctcgg gaggaacgcc cgcgtcccga acagcacacc gaggatgttc agtcggacgt
     1501 ggtcctcgat ccggtcgtag tcgagcttcg tgaactcgcc tccgccgcgc atccccgcac
     1561 tgttgacgag gacgtcggcc ggtccgtaca cctcctccgt caccacacgg agcgtcgtga
     1621 gctgttccgg ctcggtgacg tcgcaggtgg cggcaagcgc cttcccgccc ttccgctcga
     1681 tccgctcgac gatcgcatcc agccgctcgg ttcggcgcgc tgcgaggacc acgcgagcac
     1741 cgtgttccgc gaacgcgagc gccgccgcct cgccgatccc cgacgacgcc ccggcgacca
     1801 cgaccacagc tccctcgatg cgcaggacga accccctcgt acgatggtgg tgccttggac
     1861 gttatcggag cccacgccgg tcgtctgccg gtgctcgacg agcgctggga gccgcggccg
     1921 tatccacggg aggagttgga gcgcgccctc gtggccggcg gggtggcggg accggcgggg
     1981 catccgcatc acaacgtgtt cgggaacatc cggaagctcg tcgagcacga tcccgacaag
     2041 ctcttcggtc tgtcgagcat gcccgaaccc ttcggcttcg acgacatcgt gcggatcgtg
     2101 ggcgaggcct cgggcgtgcc gatcgacgcg ttggcgaccg agggatcgcc cgacatcgac
     2161 gcggcttccg tggtggacgc gtgcgagcgg gtgggggagc gcctggcggg cgccgcgcgc
     2221 cgtcgcgagc gggtgctgtt cgccaccggg caccccggcg acctcgatcc gttgtacggc
     2281 gccatcgccg agctcgcggc ggcccgcggc gccgagatcg tccgaccggc cgacggcgag
     2341 gcctggttcg acgatcacgc cggcaaggag tggacgatcg cctaccacgg cgcggtcggc
     2401 atggtcacgg acggggagcg gccgaggcac acccactggc cggaggcgat gcgtcggatg
     2461 ctggccgacg aacgaccgga tctcgtgatg gccgatcatg gactcgcggg agccgcgatc
     2521 gaagccgggg tcgagacgct ctcgatcgcc gacgtgaacg atcccgcgct gatcgtggcc
     2581 aaggcgcaag gccggaccga gatcgtggtc gtcctcgacg atcacgttcc gccggagaac
     2641 tactggccgt gcttccaggc ggtggccggg cgcttcgatc cgagctagtc gctgcgcgcc
     2701 ccgcgcggat ccggttccct gacgtcgacg atctcgtcgt cggccgggcc atcgtcgtgg
     2761 ccggcgtcgg cgagcgcctt caatcggtag gagacgccct gtcgcgtgat ctccagcgct
     2821 tcggcgatgt cctcgagtga ggcaccgagc tcgcgagcac gcaacaacgc ctcggcttcg
     2881 atggcatcga gctcggtgcg gagttcgcgg atcgcctgca gggcgaccat agggaacgcg
     2941 ctcgcgtgct cgagcttgcg cagcagtgca cgtcgggcgt ccgtcatgcg gcggagcata
     3001 cgagagaggt atgcaagaag caaggaaaga agaccgcagg agagcttgca tggagagctg
     3061 cggtggatca tccctggtcg cctcgaatga gcacggaatc gccgcgcggt gaggaaagag
     3121 gacttgatgt cggccgttcg ggggtattga cggggtcccc catctcacct acgctcctcc
     3181 atccacgcaa ctgcctacag caggcgcagt ggatgggaca c
//
LOCUS       DASUVE010000184         6054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_658950, whole genome shotgun sequence.
ACCESSION   DASUVE010000184 DASUVE010000000
VERSION     DASUVE010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658950"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1423..1797
                     /locus_tag="VF036_03930"
     CDS             1423..1797
                     /locus_tag="VF036_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HEX6264413.1"
                     /translation="MSETTVFDAAGGMPFFEQLVRLFYEGVASDPVLRPLYPDDLAPS
                     TRHLTLFLAQYWGGPTTYDEERGHPRLRMRHAPFAVGPAERDRWLVHMRAAVATMAPP
                     EPIERALVDYFEMAAEAMRNRD"
     gene            complement(1802..3496)
                     /locus_tag="VF036_03935"
     CDS             complement(1802..3496)
                     /locus_tag="VF036_03935"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX6264414.1"
                     /translation="MPERYSRSEHATRHGPTAGDRIRLGDTDLWIRIERDLTDPADQA
                     LWGYAKNWRSGMVQHDRATTESELDTIVASAVVLDPLLGVLKADIGIKDGRIAGIGRA
                     GNPAITDGVDLTIGPNTWPIPCHGLIATPGAVDSHVHLLSPRLVPVALSAGVTTLITA
                     GFEEPPWRMLRTLEAFELLPVNIGLQPSARTGVPGQLEVAIEAGAVGLKIHEDWGAYP
                     EVIDEVLRVADAHDVQVCLHTDGLNESCELAHTVEAIAGRAIHAYHVEGSGGGHIPDL
                     IGIVREPNVYCSSTTPTIPYGAATAAEQQDMKLIVHEGNPALPDDVVAARELVHPATM
                     EAEGPLHDLGAISIVNSDSQGMGRIGETVRRTFQLAHAMKAAAMQTVGGNGRDDNDLD
                     DNDRVLRYLAKVTEEPARVHGISDEVGSLFPGRIADLVLWEASHFGVKPVMVFKGGVV
                     AWTAIGEGNASVHGAEPTRYGPDWGGTGLAPARVSTTFVSASAMKAGIARTLGTRRRL
                     VAVRGTRAIRRDGLARNRTVPPIEVSAADGTVTLDGRVLRTDPVTEVRLSRRYLLG"
     gene            complement(3489..4082)
                     /locus_tag="VF036_03940"
     CDS             complement(3489..4082)
                     /locus_tag="VF036_03940"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016824089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEX6264415.1"
                     /translation="MQLTPTEEERLRVFTAAQLARSMLAKGLRLNAPEATALVCDEMH
                     VAGRGGGSFDEVVAAGRDAVTPDQVMEGIAAIVKEIRVEVLLEEGTRLFVIREPFGPA
                     GSDGPGAIRFADGDVELATGRDRIHLTVTNRGEHPIRVSSHFPFWRSNEHLTFDREAA
                     DGFRLDLPAGDSLRWAPGETHEVDLVRYGGTGAPPGA"
     gene            4128..5501
                     /gene="chrA"
                     /locus_tag="VF036_03945"
     CDS             4128..5501
                     /gene="chrA"
                     /locus_tag="VF036_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX6264416.1"
                     /translation="MARPSDGYPAAHGPTAPTFREASRFWIWLGFVNFGGPAGQIALM
                     HQELVERRRWIDEGRYLHALSYCMLLPGPEAMQLATYVGWLLHRIRGGMVAGVSFVLP
                     SFFLLLGLSWVYVTHGDLRWIAGAFAGLAAAVVGVIAHALVRLAGATLANALMVTVAI
                     VAFLAIFVIDVPFPIVIAVAAGIGIVGGIVRPSLFEVGVGRRLEEQTAVAVSDATNAP
                     LPTWRRNAVVLVVGLAAWLLPIAAVVWWRGSADTVSEMGWFFSKAAVVTFGGAYAVLS
                     YVDQAAVGTYGWLQPGQMTVGLGLAESTPGPLIMVLEFVGFVGAYQHPGDLPPIVAGL
                     LGATVTVWATFAPCFLWVFLGGPYVERLRGNARLASALQTVTAAVVGVIANLAVSFAI
                     VTLSREVRTVSILGGEIPVPVWSEIDPFAVAVATFAFVVLRFFHWHVVPVILTSALAG
                     LVVKGLL"
     gene            complement(5485..>6054)
                     /locus_tag="VF036_03950"
     CDS             complement(5485..>6054)
                     /locus_tag="VF036_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEX6264417.1"
                     /translation="RDPVLIPTAAGGEVEPYTPVRASGTFDTEREVLVYGRSLDGEPG
                     HLVVTPLVFQDGTAVLVIRGWVPFDHQTVPVTDAAAPAGELTIHGFLVPDEGGGSTAP
                     DESGVIGRLDVEGIGSTLPYPVLAPAIRQTEQAPPQPSELPIPLPQEELSEGPHLSYA
                     IQWFCFAAIAVVGAVILVRRERRSPRAAP"
BASE COUNT          782 a         2087 c         2216 g          969 t
ORIGIN      
        1 gttccggaag cacgtctcca acgcggcgag cgccgccggt cggtcgtgcg gcaggtcggc
       61 gagggcgcgg agatactgca gcgtcagcgc ggaaggcatc cgaccgagta tcgcacggtc
      121 ctcgcgaacc cttccgccgt accctttcgt accgtgaggg tacgtcgcgc gctcggactg
      181 atcggagtgg cggcgctgac cgtcgcctgt acgcaagggg gatcggcgca gccgccgggg
      241 acgcccggcc cgagccgctc gcccgccgtc acccccgacg tgggacagat ccgggcagag
      301 cgcctgctgg tgctgatgga cgacggcaac gtggtcgtcg tccgcgcgga cggcgaggat
      361 ccccaacgcc tgacggagtt cgactcggac gcggtgaccg tcgaggtgcg acatccggtc
      421 tggtctcccg acgggcggtc ggtcgcgtgg gccgagctcg agatcgggga ccagggcgca
      481 cgcccgcgca tcgtcagcac cgacccggac gggtcacggc gcaccgagtt ccccgtggac
      541 accggggcgt tcttcctgca atgggacccg acgtcgtccc gcatcgccta cctcgggaac
      601 ttccagggca gcgtggggat gggtgtcgcg ggacgcgccc cgatcgggga ccccgtcgcg
      661 acgacgctgg gggtcggacg acccttctac ctgtcctggg caccgggcgg cctggagctg
      721 ctcgtgcacg tcggcgacgc gacgctcggg cgattggatc tccaagggga gctgcgtgac
      781 ctcggcgatg cgccgggcat cttccaggct ccggtctggc tcgccgacgg gcggatgttc
      841 tacgccaccg ctgagcggga tcgccaaacg ctcgtggtcc gcgacgggga ccgactccgt
      901 gagctggtcg agttcgaggg cgcgatcgag ttcgtggtgg atccccgcgg ggagcggatc
      961 gcgtaccgcg tggacgacgt cgccggaccg ggtggggtgg aggtgatcga tacgacgacg
     1021 gcgagttcgc aggtcgtctc ggcctcgccg acgttcgcgt tccagtggag ccccgacggg
     1081 gagcggctcc tgctcttgac cccggacgag gacggcgaga ccggcgccca ccgatggctg
     1141 gtgtgggacg gcgacgaggc gcggccggtc ggcccggcgt tcgtgcccag cccgtccttc
     1201 ctccgggact acgtcccgtt ctacggacag ttcgcgcaag cgatgacgcc atggtccccg
     1261 gacggcggtg cgttcgcctt cgcggggcgg atcggcgatc ggaccggcgt ctgggtgcag
     1321 gatctccgcg ccgtcgaccc cacgttcgtg ctggaaggcg gctcggtggt ggcgtggtcg
     1381 ccgacgccga cgtgccgccc gtccgtagac tgcgcgtccg tgttgagtga gacgaccgtg
     1441 ttcgatgccg ccgggggcat gccgttcttc gagcaactcg tgaggctgtt ctacgaaggc
     1501 gtcgcgagcg atcccgtgct gcggcccctg tatccggacg atctcgcgcc gtccacccga
     1561 cacctgacgc tcttcctcgc gcagtactgg ggcgggccca cgacgtacga cgaggagcgc
     1621 ggccaccccc ggttgcggat gcgacacgcc ccgttcgcgg tgggacccgc ggagcgcgac
     1681 cggtggctgg tgcacatgcg ggcggccgtc gcgacgatgg cgccgccgga gccgatcgag
     1741 cgagcgcttg tggactactt cgagatggcg gccgaggcga tgcggaaccg ggactgatcg
     1801 ctcagccgag caggtagcgc cgcgacaacc gcacctcggt cacgggatcg gtccgcagca
     1861 cccgcccgtc gagcgtcacc gttccgtcgg ctgccgagac ctcgatcggg gggacggtcc
     1921 ggttccgcgc gaggccgtcg cggcggatcg cgcgggtccc gcggacggcc acgagccgtc
     1981 gccgcgtgcc cagcgtccgg gcgatccccg ccttcatcgc ggaggccgag acgaacgtgg
     2041 tggagacccg cgcgggcgcc agtcccgtgc caccccaatc gggtccgtac cgggtgggct
     2101 ccgcgccgtg cacgctcgcg ttgccctcgc cgatcgcggt ccacgcgacc acccctcctt
     2161 tgaacaccat cacgggcttg accccgaagt gggacgcctc ccacaggacg aggtcggcga
     2221 tccgtccggg gaagagcgag ccgacctcgt cagagatgcc gtgcacgcgg gccggctcct
     2281 cggtgacctt cgccagatag cgcaggaccc ggtcgttgtc atcgaggtcg ttgtcgtcgc
     2341 ggccgttccc gcccacggtc tgcatcgccg ccgctttcat cgcgtgcgcg agctggaacg
     2401 tccgccggac ggtctcgccg atccgaccca tcccctgcga gtccgagttc acgatcgaga
     2461 tcgcgccgag gtcgtgcagc ggtccctcgg cctccatcgt cgcgggatgg acgagctcgc
     2521 gagccgccac gacgtcgtcg gggagggcgg ggttgccctc gtgcacgatg agcttcatgt
     2581 cctgttgctc ggccgcggtc gcggcgccgt aggggatcgt cggtgtggtc gacgagcagt
     2641 agacgttcgg ctcgcggacg atcccgatca ggtccgggat atggccgccc ccggagcctt
     2701 cgacgtggta cgcgtggatc gcacggccgg cgatcgcctc caccgtgtgc gcgagctcgc
     2761 acgactcgtt gagcccgtcc gtgtgcaggc agacctggac gtcgtgcgcg tccgcgaccc
     2821 ggagcacctc gtcgatcacc tccgggtagg cgccccagtc ctcgtggatc ttgagtccga
     2881 cggcgcccgc ctcgatcgcc acctcgagct gcccagggac cccggtccgg gctgacggct
     2941 ggagcccgat gttcacgggg aggagctcga aggcctccag ggtgcggagc atccgccacg
     3001 ggggttcctc gaaccccgcg gtgatgagtg tggtgacacc ggccgagagc gcaaccggca
     3061 cgagccgggg cgagaggagg tgcacgtgcg agtcgacggc gcccggcgtg gcgatcagac
     3121 cgtggcacgg gatcggccac gtgttcggcc cgatcgtgag atcgacgccg tcggtgatgg
     3181 ccgggttgcc cgccctcccg atcccggcga tccggccgtc cttgatgccg atgtccgcct
     3241 tgaggacgcc gaggagcggg tccaacacga ccgcgctcgc gacgatcgtg tcgagctcgc
     3301 tctcggtcgt cgcgcggtcg tgttggacca tccccgagcg ccagttcttc gcgtagcccc
     3361 agagcgcctg gtcggccggg tccgtgaggt cacgctcgat ccggatccag agatcggtgt
     3421 cgcccaggcg gatgcgatcg ccggcggtcg gaccgtggcg cgtcgcgtgc tcggaacgcg
     3481 aataccgctc aggcacccgg cggagccccc gtccctccgt aacggacgag gtccacctcg
     3541 tgcgtctcgc ccggcgccca ccgcagggag tcgcccgcgg gcaggtcgag ccggaatccg
     3601 tcggccgcct cgcggtcgaa cgtgaggtgc tcgttcgaac gccagaacgg gaagtgcgag
     3661 gacacacgga tcgggtgctc gccgcggttc gtcacggtca gatggatccg gtcacgaccc
     3721 gtggcgagct cgacgtcgcc gtcggcgaat cggatcgcgc cgggtccgtc ggaccccgcg
     3781 ggaccgaacg gctcccggat cacgaacagc cgagtgccct cctcgagcag gacctctacg
     3841 cggatctctt tcacgatcgc ggcgatgccc tccatcacct ggtcgggcgt gacggcgtcc
     3901 cggccggccg cgacgacctc gtcgaacgag cccccgccgc ggcccgccac gtgcatctcg
     3961 tcgcacacga gcgcggtcgc ctcgggcgcg ttcagccgca ggcccttcgc gagcatcgag
     4021 cgggccagct gggcggcggt gaagacccgc agacgctcct cctcggtggg ggtcagttgc
     4081 atcggctccc ctcttctatg ccgctggcga gcgtaccctc ggtcgcgatg gcacgtccct
     4141 cggacgggta cccggcagca cacggcccca cggcccctac gttccgcgag gcgtctcgct
     4201 tctggatctg gctcggtttc gtgaacttcg gcggtcccgc ggggcagatc gccctgatgc
     4261 atcaggagct cgtcgagcgc cggaggtgga tcgacgaggg tcggtacctt cacgcgctca
     4321 gctactgcat gctgcttccg ggtccggagg cgatgcagct cgccacgtac gttggctggc
     4381 tgctccaccg catccggggt ggcatggtcg cgggggtctc gttcgtcctg ccgagcttct
     4441 tcctgctcct gggcctgtcg tgggtgtacg tgacgcacgg ggatctccgg tggatcgcgg
     4501 gggcgttcgc cgggctcgcc gcggctgtcg tcggtgtcat cgcgcacgcg ctcgtccgcc
     4561 tcgcgggcgc gacgcttgcc aacgcgctga tggtcacggt cgcgatcgtg gcgttcctgg
     4621 cgatcttcgt catcgacgtt cccttcccga tcgtgatcgc cgtcgcggcg gggatcggca
     4681 tcgtgggcgg gatcgttcgc ccctcgctgt tcgaggtcgg agtcggacgg aggctcgagg
     4741 agcagacggc ggtggccgtc agcgacgcta cgaacgcgcc gctgccgaca tggcgacgca
     4801 acgccgtggt cctggtggtc ggcctggcgg cgtggctcct cccgatcgcg gcggtcgtgt
     4861 ggtggcgggg gagcgcggac acggtctcag agatgggctg gttcttctcc aaggccgcgg
     4921 tcgtcacgtt cggcggcgcg tacgccgtgc tctcctacgt cgaccaggcg gccgtcggga
     4981 cgtacggatg gctccagccc ggccagatga cggtcggcct cggtctggcg gagtcgaccc
     5041 cagggccgct gatcatggtg ttggagttcg tgggcttcgt gggcgcgtac cagcatccgg
     5101 gcgatctccc gccgatcgtc gccgggctgc tgggcgcgac cgtgacggtg tgggcgacgt
     5161 tcgcgccgtg cttcctgtgg gtgttcctcg gaggcccgta cgtcgagcgg ctgcgaggca
     5221 acgctcggct cgcgtcggcg ctccagaccg tcaccgcggc ggtcgtcggg gtgatcgcga
     5281 acctggccgt gtcgttcgcg atcgtgacgt tgtcccggga ggtgcggacg gtatcgatcc
     5341 tcggcgggga gatccccgtc ccggtctggt cggagatcga cccgttcgcg gtggcggtcg
     5401 cgacgttcgc gttcgtggtc ttgcggttct tccactggca cgtcgtcccg gtgatcctga
     5461 cgagcgccct cgccggcctc gtggtcaagg ggctgctcta ggagagcgcc gctcgcgccg
     5521 caccaggatg acagctccga cgacggcgat ggcggcgaag cagaaccact ggatcgcgta
     5581 cgacaggtgc ggcccctcgg acagctcctc ctgcgggagc ggtatcggaa gctccgaggg
     5641 ctgcggcgga gcttgctctg tctgccggat cgcgggagcg agcactgggt agggaagcgt
     5701 cgagccgatg ccctcgacgt cgagccggcc gatcacgccg ctctcgtccg gagcagtgga
     5761 gccgccgccc tcgtcgggca cgaggaaccc gtggatcgtg agctccccgg cgggcgccgc
     5821 cgcgtcggtc acgggcacgg tctgatgatc gaaggggacc catccccgga tcaccagcac
     5881 cgcggtcccg tcttggaaca cgagcggcgt cacgacgagg tggcccggct cgccgtcgag
     5941 gctgcgcccg tagacgagca cctcacgctc ggtgtcgaag gtcccggacg cgcggaccgg
     6001 ggtgtacggc tcgacttcgc cgccggcggc tgtcgggatg agcaccgggt cgcg
//
LOCUS       DASUVE010000185        12431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_659352, whole genome shotgun sequence.
ACCESSION   DASUVE010000185 DASUVE010000000
VERSION     DASUVE010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12431
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659352"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VF036_03955"
     CDS             <1..261
                     /locus_tag="VF036_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264418.1"
                     /translation="FTIFLSILGIVALQVLRLPKWDRNFWLTSLFVWAIGASTLTWEH
                     RKTTWLFLTFAVVAAVIGARRSAAPDHVIDLTDHDVVKVGLV"
     gene            258..1145
                     /locus_tag="VF036_03960"
     CDS             258..1145
                     /locus_tag="VF036_03960"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX6264419.1"
                     /translation="MTLPTFLGIGVPRAGTTWLHQLLSAHPDVYLPSRRKEVRFFDRH
                     HDEGRGWYEGFFCPPDEAGRYSAIGEISPQYLYCDACPERISTLLPEAKLLVMLRHPV
                     DRAYSQFGFVIQRRDFRGSFEEFVATRPRALEMGFYSRYLERYLDHYERQRILAVLFE
                     EAVSEGSGVRGELAAFLGVDEDRFPDATERVNPSTVPRFRTISSAAVKVGRRMRRHNL
                     EWLVDVAGRMGLRRALTSGRRVPPLDPQLRRELSGQYETEFETLERSWGLDLARWKAP
                     RAAAPLRENTAGVPRGGAS"
     gene            1142..2338
                     /locus_tag="VF036_03965"
     CDS             1142..2338
                     /locus_tag="VF036_03965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6264420.1"
                     /translation="MNRPLNVLIVSQPVSYGVAICVRDLTQAAVAAGHRVTVVSPDSA
                     HGPLAGWIEAAGARHHPLNMARAPAPRDLSDLLVLRRLSRGRDVVHLHSSKAGALGRV
                     AAMSLGRRHRPAVVFTAHYWSWLVGGRWARLYRLIEGVLARRCDAIVAVSDGEAEEGR
                     AVLGARVPLRSIPNGVDRDRFSPIGDRADRTEDVPLIVCVGRLSEQKGQDVAIRALAL
                     LPDRTARLRLVGDESGHGERTRLDALAASLGVSDRIEWRGGVDATAPEYRAADVVVAP
                     SRWDGLSLALLEGMACGAAIVASEVNGSECLGEAGRIVPPDDPESLAEVVAGLLGSPA
                     ERARLGQAARERSRSFDLATTMGRNLDLWSGLAVRAAGGRDAAAAQAVADGGEGAARW
                     TNTSTS"
     gene            2314..3288
                     /locus_tag="VF036_03970"
     CDS             2314..3288
                     /locus_tag="VF036_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX6264421.1"
                     /translation="MDQHLDVLTTERPAMDAPIPYVCMPGSPYTGSTLLGMLLGNHPE
                     CASIGAATGLTARVDVSTYLCSCGARFTDCGFWGRIARRTVELGYPVTVFEKDFWNTH
                     VRVSRRRWLNGLLVSSLGNDMLTWSRDAVLHRAGPVARRIAEARSASWSLARAVLEQT
                     GKGTFVDTARDHQRPRYLADMPRLDVKVIHLVRDPRGNVASIMKHTKVGVEKAARQWR
                     HYNVEADRVRRRFPRDSWMLLYYEELCAEPQASLDRVARFIGVQPAPITQLLENSERH
                     VIGNSMRLKVLDEIREDLSWQPTLSAVDLRTIARIAGRTSRRLGYDWP"
     gene            3285..4139
                     /locus_tag="VF036_03975"
     CDS             3285..4139
                     /locus_tag="VF036_03975"
                     /inference="COORDINATES: protein motif:HMM:NF018669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264422.1"
                     /translation="MSATRPLLVFVHINKTAGTTLRYILRSSYGSRHCDVEPWHGAWV
                     DPPFSTEDLRRVRKLYPRLASIAGHRISGHVDLAEEGTDFRYLTFLREPIALCASRFQ
                     YQLDYRKKQDLVFEDWIEKAWARDAQTQRIGGTTDPSDAIRVIERKSMFVGLTERFDE
                     SIVLLRAQRAPDLNIAYAPVNVAKSSDIAKGLLADPRTRQMIVEANAADLELYEHVMR
                     EIYPAQQRAYGASLSNTVATFREGVQQRYDRRKLALYGLKQRAMLKPTLRLYRGRRTG
                     GVVRALIG"
     gene            complement(4144..5013)
                     /locus_tag="VF036_03980"
     CDS             complement(4144..5013)
                     /locus_tag="VF036_03980"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX6264423.1"
                     /translation="MIIGAAKAGTTALYWYLADHPEVFMSPVKETNYFAFGLDDGGRP
                     LYGDPELHRFPIRSFAEYEALFAGSDGALAVGEASPIYLECPQSAARIHEVVPEARIV
                     CFLREPVDRAYSDYLMYLRARGRRLDPDRDLTPDAAWARPDSHWMQISRYHEALSRYY
                     ERFPREQIDVFLFDDLKTDAAGCVRAVFAALGVDPGFTPDLETPHNIGGLPSSRTLER
                     VLTSRSVRKVVEPWIPRRVSDAVRRVRTKNLKKAPPLPDQLRAELLRHFQDDIGRTAA
                     LIGRNLDHWLPPV"
     gene            complement(5038..6519)
                     /locus_tag="VF036_03985"
     CDS             complement(5038..6519)
                     /locus_tag="VF036_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264424.1"
                     /translation="MIVELIGATGAGKSTLAHELLRRGLESGQVRMATDLVTDRPGRR
                     WVENPHAVNLLADAAALPPFLRSFDDHRDFVRFAFERLRHHAPSTFAKANYLRNVVRK
                     VGVHELTRRADPAVTFLNDEGTVLIAYQLFVYSDAAPTPSEIERFARLVPLPDLVVHV
                     KAPTDLLVERAKSRPDVRRELAGLDGERIGSWVGRAVSVFDALAESNAMRDRVVTIEL
                     EDASPEGLDRAVEQLRDAVETRSRALDASTRSALDGRSGASRRATVIAFVGSEATGKS
                     TVLDEVERWLSKSRRVRRVHAGKPPSTPLTSIPHALLPALRSLFPEQRTLRVEERYEG
                     GDATGTTYPLLFGIRSVMLAYERRALIRRAVRDGAGTVVLSDRYPSDASGAPDGPQLT
                     HLPMPKSAFSLRRILASIEGRLYRGIPTPDLVFQLTAPLEVTLARNAAREKKEPEEYV
                     RFRHELSSRLRFDGASVYPIDTDRDLDQVVREVQVLIDARREV"
     gene            complement(6516..7616)
                     /locus_tag="VF036_03990"
     CDS             complement(6516..7616)
                     /locus_tag="VF036_03990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6264425.1"
                     /translation="MLHLIIMLGETNGQYNEHCLPLMGERDISIVTYFVPKLTPPPAI
                     TLFAGDGTVRGFFRALKAALRAKYDIIHAHAPQTGTLFVLATLAWRRFSRVRSSLVYT
                     VQDSFHDYKLRNQLMMIISLAAFRRIVFCSRSAYESLPGALDRLVRGRSRVVPNGADL
                     DRVDRALATRAPAPSGGGFTVISVGRLERVKDPLTLVNAFARAVGDDAGSRAILVGAG
                     ELSTTIEARARELRLPDRVELTGLVPRDDVFVACADADVFVSTSHGEGLPVAVLEAMG
                     AGCPVILSDIPPHRELADGADFIPFADPGDVDGFADAIRRFHAMTPIERREVGRRCRE
                     HMAARFTLPIMRAGTEAVYREVVPLVGSGAAG"
     gene            complement(7646..8371)
                     /locus_tag="VF036_03995"
     CDS             complement(7646..8371)
                     /locus_tag="VF036_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264426.1"
                     /translation="MTRIVEIAVGRPGPTHRADRNIEILGVAGAGKSTLATRLAAEPG
                     FEIAPFIHARRPGDLLLIIRTIPRLLPILWMGLTRAPRISWPESKLLVYVTRWAPVLR
                     RRRPQTDTALLLDQGPLYAIVRLMAEAKPFTEHRSFTTWKREMLDRWSTELSDVIWLD
                     APDAVLWSRINQRDQDHRQKGGQADAGHRFISRYRRAFESIVPIVEDKGVVRTLRFDT
                     STSTVEQIVEGVRASRRRGGEPA"
     gene            8646..10547
                     /locus_tag="VF036_04000"
     CDS             8646..10547
                     /locus_tag="VF036_04000"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX6264427.1"
                     /translation="MTSILKHAARGGRISVVLAVSLVVIGVALPAFANLPDTSTPSLE
                     LDRTITTQPFVGTSTTMRDGEGSAYVPQDGSLWLTEDHDNFLFETNPLTGALKRVINN
                     TAFQNAPQFGGGPVAGPNRTDDFESMAYDEANDWLFVFSGPCCDASHLPTVFRLVRVG
                     GIFQVESYQPLAATANHTASAWSPADQKLYVGQGSSIRTYDYVTNTTGTAFSVTGLSG
                     ILGFSFSSVGDTGGSDLFVVTNAERLRRVDWATKTLVPGWTFDLTPFDVLDSRAVELI
                     GDQFYVLDGADARPPGPLRFAVFVFDVLGPQPVAPVASFTAAPMSGNAPLSVDFDDTS
                     TNNPTSWAWDFETDGIVDSTAQNPTHVYQTAATYTVTLTATNGAGSDSETAQITVTQA
                     VAPVASFTATPSTGNAPLSVSFTDTSSGNPTVWAWDFESNGSVDSNAQNPTHIYTTPA
                     TYTATLTVTNGAGSDSETAQIVVNPPPQPTNLVGNPGFDVDTAGWSTAGSGTLVTLTR
                     VTPGRDGTGGAARLFNGASGTRKCVLNDQPNWVTTTTAGRYTASIWVRGDVAGALIKI
                     NIREMNGSTVVKSRVGNFTLTTAWQQITVAHTILSPGSTLDLQVFLPKAQAPPGTCFY
                     ADDASIVRS"
     gene            10728..12116
                     /locus_tag="VF036_04005"
     CDS             10728..12116
                     /locus_tag="VF036_04005"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX6264428.1"
                     /translation="MALHEPLEIAFAPTPPSLRSRPPLSSLLPVMDALTLIGVSAILG
                     FQLVSIAYGVATFLILNGDSSRKYRINPRLGDEAGWLLGRVSVALLALLSVGWVAQLF
                     HVDLLGSEIRELTKVGLAVAGAVFLGRGAAYALGRKAKERGLASERTLIVGDGELAMG
                     LAETFLEHPEYGLRPIGFLGRPLVRHVALPQLGEVRNVEQVIGDFEVRRVLVAFGEMR
                     DQELVTILRASERVPAEIHVVPRFFELSGVPQGAPVDDVAGIPLFHLRRPALRPGARF
                     QKRAFDMVVASMLFLLSLPVFAVIALAVRLSSEGPVLFRQKRVGLNGNLFEIIKFRTM
                     YVNDDSDTEWITDRDRDRVTPIGHVLRAASLDELPQLINVIRGEMSLVGPRPERPHYT
                     GRFSETVSRYDDRHRVVGGITGWAQINGRSRGLDSVPERARLDNYYIENWSLWRDLVI
                     VARTVAVLFRGR"
     gene            complement(12118..>12431)
                     /locus_tag="VF036_04010"
     CDS             complement(12118..>12431)
                     /locus_tag="VF036_04010"
                     /inference="COORDINATES: protein motif:HMM:NF023385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HEX6264429.1"
                     /translation="PVGSVRVPWAGGAYFRLIPFPVFRRGVARRLRSTSWFTFYLHPW
                     ELDAEEQRLPQMARSLRLRAYTGRGRMRRDLRRLIEEFGSTRIDEALMARGHQPPGGT
                     A"
BASE COUNT         1952 a         4181 c         4298 g         2000 t
ORIGIN      
        1 ttcacgatct tcctgtcgat cctcgggatc gtcgccctcc aggtgctgcg gctcccgaag
       61 tgggaccgaa acttctggct gacctcgctg ttcgtatggg cgatcggcgc gtccaccctc
      121 acgtgggagc accggaagac cacgtggctc ttcctcacgt tcgccgtggt cgcggcggtc
      181 atcggcgctc gacgctcggc ggcgccggac cacgtcatcg atctcaccga ccacgacgtg
      241 gtgaaggtcg gactcgtatg accctgccga ccttcctcgg gatcggcgtg ccccgcgccg
      301 ggacgacgtg gctgcatcag ctcctctcgg cgcaccccga cgtgtacctg ccctcgcgcc
      361 gcaaggaggt gcgcttcttc gatcgccacc acgacgaagg acgggggtgg tacgaggggt
      421 tcttctgccc tcccgacgag gccggccggt actcggcgat cggtgagatc tctccccagt
      481 acctgtactg cgatgcatgc ccggaacgga tctccacgct gcttcccgag gcgaagctcc
      541 tcgtgatgct ccggcacccg gtggatcggg cgtactcgca gttcggcttc gtgatccagc
      601 ggcgggactt ccgcggctcc ttcgaggagt tcgtcgccac gaggccccgg gcattggaga
      661 tgggcttcta cagccggtac ctcgagcgct accttgatca ctacgagagg cagcggatcc
      721 tcgcggtcct cttcgaagaa gccgtatccg aggggagcgg ggttcggggg gaactggccg
      781 cgttcctggg cgtggacgag gaccgcttcc ccgatgccac cgagcgggtc aacccgagca
      841 cggttcctcg cttccggacg atctcgagcg ccgccgtcaa ggtcggtcgg cgcatgcgga
      901 ggcacaacct tgagtggctg gtggatgtgg ccggccgcat gggcctgagg cgcgcattga
      961 ccagcggtcg tcgcgtgccg ccgctggatc cgcagctgcg gcgggagctg agcggtcagt
     1021 acgagacgga gttcgagact ctcgagcgct cgtgggggtt ggatctggcg aggtggaagg
     1081 ccccccgagc cgccgcccct cttcgggaga acaccgccgg ggtcccgcgc gggggggcat
     1141 cgtgaatcgc ccgctgaacg tcctgatcgt ctcgcagccc gtgagctacg gggtcgcgat
     1201 ctgcgtccgc gatctgacac aggcggccgt cgcagccggc caccgggtca ccgtggtgag
     1261 ccccgattcc gcgcacggtc cgctcgccgg ctggatcgag gccgccgggg cccgacacca
     1321 cccgctgaac atggctcggg cacccgcccc cagggacctc tccgacctcc tggtcctccg
     1381 gaggctgagc cgcggccgag acgtggtcca tctccattca tccaaggcag gtgcgctcgg
     1441 acgcgtcgcg gcgatgtccc tcggacgacg ccaccggccg gcggtcgtct tcaccgccca
     1501 ctactggtcc tggctcgtgg gcggtcgctg ggctcgcctc tatcgattga tcgaaggggt
     1561 gctcgctcgc cggtgcgatg cgatcgtcgc ggtctcggac ggcgaggcgg aggaaggtcg
     1621 cgccgtgttg ggcgctcgcg tgccgttgcg atcgatcccc aacggggtcg atcgggatcg
     1681 gttctctccg atcggcgacc gagccgaccg gaccgaagac gtgccgctca tcgtctgcgt
     1741 gggaaggctg agcgaacaga agggccagga cgtggcgatc cgcgcgctcg ccctcctgcc
     1801 cgatcgaacc gcacggctgc ggctcgtcgg cgacgagagc gggcacggag agcgcacgcg
     1861 gctcgacgcc ctcgcggcct cgctcggcgt ctccgaccgg atcgaatggc ggggtggcgt
     1921 cgatgcgacg gcgccggagt accgcgccgc cgacgtggtc gtcgccccat cgcggtggga
     1981 cggtctctcc ctcgccctcc tcgaggggat ggcgtgcggg gcggcgatcg tcgcgagcga
     2041 ggtgaacggg agcgagtgcc tcggagaggc cggccggatc gtgccgccgg acgatccgga
     2101 gtccctcgct gaggtcgtgg ccggtctcct cggaagccct gcggagagag cgcggctggg
     2161 ccaggccgcc cgagagcgca gccgctcctt cgatctcgcc acgacgatgg gacgaaacct
     2221 cgacctgtgg tcgggcctcg cggttcgtgc cgccggtggc cgggatgccg cggccgccca
     2281 ggcggtcgcc gacggcgggg aaggagctgc tcgatggacc aacacctcga cgtcttgacg
     2341 accgaacgac cggcgatgga cgccccgatc ccctacgtgt gcatgccggg cagcccgtac
     2401 acgggttcga cgctcctcgg gatgctcttg gggaaccatc cggaatgcgc gtcgatcggc
     2461 gcggccaccg ggctcaccgc gcgggtcgat gtctcgacgt acctctgctc gtgtggcgcg
     2521 agattcaccg attgcgggtt ctggggccgt atcgcccgga gaaccgtgga actcgggtat
     2581 ccggtgacgg tgttcgagaa ggacttctgg aacacgcatg ttcgggtctc gcgacggcga
     2641 tggttgaacg ggctcctcgt gagctccctc ggcaacgaca tgctgacctg gtctcgggac
     2701 gccgtgctgc atagggccgg gccggtggcg cggcggatcg ccgaggctcg atccgcttcg
     2761 tggagcctgg cacgcgccgt cctggagcag acggggaagg gaacgttcgt ggacacggct
     2821 cgtgatcacc agcgtcctcg ctatctggcc gatatgccgc gcctcgacgt gaaggtcatc
     2881 cacctcgtgc gggacccgcg gggcaacgtg gcgagcatca tgaagcacac caaggtcggc
     2941 gtcgagaagg cagcccgtca atggcggcac tacaacgtcg aagcggatcg ggtgaggcga
     3001 cgcttccccc gcgactcgtg gatgctcctg tactacgagg agctctgcgc ggaaccacag
     3061 gcgtcgctcg accgcgtggc ccggttcatc ggcgtgcagc cggcgccgat cacgcagctg
     3121 ctcgagaact cggaacgaca tgtgatcggt aactcgatgc gcctcaaggt gctggacgag
     3181 atcagggaag atctgtcgtg gcaacccacc ctgagcgcgg tcgatctccg gacgatcgcg
     3241 cggatcgcgg gacggaccag ccggcggctc gggtacgact ggccatgagc gcgacgcgac
     3301 cgctgctcgt gttcgtgcac atcaacaaga ccgcaggcac gacgcttcgg tacatcctcc
     3361 gcagctccta cgggtcacga cactgcgacg tcgagccgtg gcacggtgca tgggtcgatc
     3421 ctccgttctc cacggaggat ctccgccgcg tgcgcaagct ctacccgcgc ctcgcgagca
     3481 tcgcgggaca tcggatcagc ggccacgtcg atctggcgga ggaggggacc gacttccggt
     3541 acctgacgtt cctccgcgag ccgatcgcgc tgtgtgcttc tcggttccag taccagctgg
     3601 actaccggaa gaagcaggac ctcgtgttcg aggattggat cgagaaggca tgggcgcgag
     3661 acgcgcagac gcagcggatc ggcgggacca ccgacccttc ggatgcgatc cgcgtgatcg
     3721 aacggaagtc gatgttcgtg ggcctcacgg agcggttcga tgaatcgatc gtgctcctgc
     3781 gagcccaacg cgcccccgat ctgaacatcg cctatgcacc ggtcaacgtg gccaagagct
     3841 ccgacatcgc gaagggactg ctggccgacc cgaggacgcg ccagatgatc gttgaagcga
     3901 acgcggccga tctcgagctc tatgagcacg tgatgcgcga gatctacccg gcccagcagc
     3961 gggcgtacgg ggcctcgctg tcgaacacgg ttgcgacctt ccgagaggga gtgcagcagc
     4021 gatacgaccg ccggaagctc gcgctctacg gcctgaagca gcgtgcgatg ctgaagccca
     4081 cgctccgcct ctaccgaggg aggaggacgg gcggggtcgt tcgagcgctg atcgggtgaa
     4141 cggtcagacc ggagggagcc agtgatccag gttcctgccg atcagcgccg ccgtccggcc
     4201 gatgtcatcc tggaagtgcc gaagcaactc cgctcgtagc tgatcgggga gcggaggcgc
     4261 cttcttcagg ttcttcgttc ggacccgtct gacggcatcc gacaccctcc gtgggatcca
     4321 gggctcgacg accttccgga cggatctgct cgtgaggacg cgttcgaggg ttcggctcga
     4381 cgggagccca ccgatgttgt gcggcgtctc gaggtccggg gtgaacccgg gatcgacgcc
     4441 gagcgcggcg aagacggcgc ggacgcaccc ggcggcgtcc gtcttgagat cgtcgaagag
     4501 gaacacgtcg atctgctccc gcgggaaccg ttcgtagtac cgggagagcg cttcgtggta
     4561 cctgctgatt tgcatccagt gggagtcagg acgcgcccag gcggcatcgg gcgtcaggtc
     4621 ccggtcgggg tccaatctcc gaccccgcgc gcggaggtac atcaggtagt ccgagtacgc
     4681 gcgatcgacg ggctcgcgta ggaagcagac gatccgggcc tcgggcacca cctcgtggat
     4741 ccgggcggcg gactgcgggc actccaggta gatcggtgat gcctcgccca cggcgagtgc
     4801 cccgtccgat ccggcgaaga gtgcttcgta ctcggcgaag gatcggatcg ggaaccggtg
     4861 gagctccgga tcgccgtaca ggggacggcc gccgtcgtcg agtccgaacg cgaagtagtt
     4921 cgtctccttc accgggctca tgaagacctc ggggtgatcg gccaggtacc agtacagcgc
     4981 ggtcgtgcct gctttcgcgg caccgatgat cacgaaggtg ggaagcatcg cggtcgctca
     5041 gacctcccgg cgagcgtcga tcagcacctg gacctcccgg accacctgat cgaggtcgcg
     5101 atccgtgtcg atggggtaca cggacgctcc gtcgaaccgg agcctcgagc tgagctcgtg
     5161 acggaagcgt acgtactcct ccggctcctt cttctccctc gccgcgttcc gggccagcgt
     5221 cacctcgagt ggggccgtca gctggaagac cagatccggc gtggggatac cgcgatacag
     5281 acgcccctcg atcgacgcga ggatccgtcg caacgagaag gcgctcttcg gcatcggcag
     5341 atgggtgagc tgcgggccgt cgggcgcgcc gctcgcgtcc gacgggtatc ggtcgctcag
     5401 cacgaccgtc cccgctccgt cgcgtaccgc ccgcctgatg agggcgcgcc gctcgtacgc
     5461 gagcatcacg gatcggatcc cgaagaggag cgggtacgtc gtgccggtcg catcgccccc
     5521 ttcgtagcgt tcctcgacgc gcagcgtccg ttgttccggg aacagcgagc ggagcgcggg
     5581 cagcagcgcg tgcgggatgg aggtcagggg cgtgctcggt ggcttgccgg catggacgcg
     5641 gcgaacgcgc cggctcttcg acagccatcg ctccacctcg tcgaggacgg tcgacttgcc
     5701 cgtcgcctcc gatccgacga acgcgatcac ggtcgccctg cgggatgcgc cggaccggcc
     5761 gtcgagggcc gagcgggtcg aggcgtccag cgcgcgcgac cgcgtctcca ccgcgtcgcg
     5821 tagctgctcg acggctcgat cgagaccctc gggggaggca tcttcgagct cgatggtcac
     5881 cacacggtcg cgcatcgcgt tggactcggc cagcgcatcg aagaccgaga ccgccctccc
     5941 gacccatgat ccgatccgct ccccatcgag ccccgcgagc tccctccgaa cgtccggacg
     6001 gctcttcgct cgctccacga gcaggtcggt cggcgccttc acgtggacga cgaggtcggg
     6061 aagcgggacg aggcgggcga agcgctcgat ctcggacggc gtgggtgctg cgtcgctgta
     6121 gacgaacagt tggtacgcga tcaggaccgt cccctcatcg ttcaggaacg tgacggcggg
     6181 atcggctcgc cgtgtcagct cgtgcacgcc gaccttccga acgacgttcc ggagatagtt
     6241 cgccttggcg aaggtcgacg gtgcatggtg gcggagccgt tcgaacgcga accggacgaa
     6301 gtcccggtga tcgtcgaacg acctgaggaa cgggggcagc gcggcagcgt cggccagcag
     6361 gttgaccgcg tgggggttct ccacccatcg gcggccgggc ctgtcggtca ccaggtccgt
     6421 cgccatccgg acctggccgc tctcgagccc ccggcgcagc agctcgtggg ccagcgtcga
     6481 cttgccggcg ccggtggcgc cgatgagctc gacgatcatc ccgccgcccc gctcccgacc
     6541 agcggcacga cctctcggta gaccgcctcg gtgccggccc gcatgatcgg caacgtgaag
     6601 cgcgccgcca tgtgctccct gcagcggcgc ccgacctccc ggcgctcgat cggcgtcatc
     6661 gcatggaagc gacggatcgc gtctgcgaat ccgtcgacgt ctccgggatc ggcgaacggg
     6721 atgaagtccg caccgtccgc gagctcacgg tgcggcggga tgtccgagag gatcaccggg
     6781 cagcccgcgc ccatcgcctc caggacggcc accgggagcc cttcaccgtg tgaggtcgag
     6841 acgaagacgt cggcatcggc gcaggcgacg aagacgtcgt ctcgtggaac gaggcccgtg
     6901 agctcgaccc ggtcgggcag gcgcagctcg cgggccctcg cctcgatcgt ggtcgaaagc
     6961 tcgcccgcac cgacgaggat cgctcgactc cccgcgtcat cgccgaccgc gcgtgcgaat
     7021 gcgttcacca acgtgagcgg gtccttgacc cgctcgaggc ggcccaccga gatgacggtg
     7081 aacccgcctc cgctcggcgc gggagctcgt gtggcgagcg cacggtccac gcgatcgagg
     7141 tcggctccgt ttgggaccac ccgcgaccgt ccgcgtacga ggcggtcgag ggcgcccggg
     7201 aggctctcgt acgccgaccg gctgcagaac acgatccgcc ggaaggccgc cagggagatg
     7261 atcatcatca gctggttccg gagcttgtag tcgtggaagg agtcctgcac ggtgtagacg
     7321 agcgaggaac gcacgcgcga gaagcggcgc caggcgagcg tggccagcac gaagagggtc
     7381 ccggtctgcg gcgcatgcgc atggatgatg tcgtacttcg cgcgcagcgc cgccttcaga
     7441 gcccggaaga acccgcgcac ggtgccgtcg cccgcgaaga gggtgatcgc cggagggggc
     7501 gtcagcttcg ggacgaagta ggtgacgatc gagatgtccc gttcgcccat gagcgggagg
     7561 caatgctcgt tgtactgccc gttcgtctcg ccgagcatga tgatcaggtg cagcacacgg
     7621 agcttccggg ggggcgcgct atccgtcagg ctggttcccc tcccctccgt cgcgacgctc
     7681 ggacaccctc gacgatctgc tcgaccgtgg aggtgctcgt gtcgaaccgc agcgtgcgga
     7741 cgacaccctt gtcctccacg atcggcacga tgctctcgaa cgcccggcgg tagcgagaga
     7801 tgaatcggtg tccggcgtcc gcctgtccgc ccttctgccg gtgatcctgg tcgcgctggt
     7861 tgatccgact ccagaggacg gcgtcgggtg cgtccagcca gatgacgtcg ctcagctcgg
     7921 tcgaccatcg atcgagcatc tcgcgcttcc aggtcgtgaa ggagcgatgt tcggtgaacg
     7981 gtttcgcttc cgccatcagg cggacgatcg cgtacagggg cccctgatcg agcaggaggg
     8041 cggtgtcggt ctgcggccgc ctgcgacgca gcacgggagc ccatcgggtg acgtacacga
     8101 gcagcttgga ctccggccaa ctgatccgcg gtgcgcgcgt gagacccatc cacaggatcg
     8161 gcagcagccg agggatcgtg cggatgatca ggagcaggtc gcctggccgg cgcgcgtgga
     8221 tgaagggggc gatctcgaac cctggttctg cggcgagccg cgtggcaagc gtggacttgc
     8281 ccgcgcccgc gacgcccagg atctcgatgt tccgatccgc tctgtgggtc ggcccgggcc
     8341 ggccgaccgc tatctcaacg atccgcgtca agggccctcc gaccggatga gtggtccctc
     8401 aagggtagag ggagcacgcg gagacaacca tctgatgatc agcgtagccc gggtacgcga
     8461 tccgtcggat tccccagacg agccggaatg ttgcgcacgg ttgctcgggt ggcggaggca
     8521 tcggggagga cacatcacgt aggcgatctg cgtcgaacgc atgatgtcgt cgcgcctccc
     8581 cgccgaccag actgttgggg tcgcggggcg cgatagatgc acgacggcca gggtgggact
     8641 tctttgtgac gagcatcctc aaacacgctg ctcggggggg caggatatct gtcgtcctgg
     8701 cggtctccct cgtcgtcatc ggggtcgcgc tgccggcctt cgcgaacctc cccgatacct
     8761 ccacgccttc cctcgagctc gatcggacga tcacgaccca gccgttcgtg gggacctcga
     8821 ccacgatgcg ggacggggaa ggcagcgcgt acgtcccgca ggacggatcg ctctggctca
     8881 ccgaggacca cgacaacttc ctcttcgaga cgaacccgct caccggtgcg ctgaagcggg
     8941 tcatcaacaa caccgcgttc cagaacgcgc cgcaattcgg cgggggcccc gtggccggtc
     9001 ccaaccggac ggacgacttc gagtcgatgg cctacgacga agccaacgac tggctcttcg
     9061 tcttctcggg tccgtgctgt gacgcgtcgc acctgcccac cgtgttccgg ctcgtccgtg
     9121 tcggtgggat cttccaggtc gagtcgtacc aaccgctcgc ggcgacggcg aaccacacgg
     9181 catccgcgtg gagtccggcc gaccagaagc tctacgtcgg ccagggatcg tcgatccgta
     9241 cctacgacta cgtgacgaac accaccggga cggcgttctc ggtgaccggt ctcagcggga
     9301 tcctgggctt ctccttctcc tcggtcgggg acacgggtgg cagcgatctg ttcgtggtca
     9361 cgaacgccga acggctccgc cgcgtggact gggcgacgaa gaccctcgtt cccggctgga
     9421 cgttcgatct gacgccgttc gacgtgctcg acagccgcgc ggtggagctg atcggcgatc
     9481 agttctacgt gctggacggc gccgacgctc gcccgcccgg tccgctccgg ttcgcagtct
     9541 tcgtcttcga cgtgctcggg ccccagcccg tggctccggt ggcgtccttc accgcggcgc
     9601 cgatgagcgg caacgctcct ctcagcgtgg acttcgacga cacgtcgacg aacaacccga
     9661 ccagctgggc gtgggacttc gagaccgacg gcatcgtcga ctcgacggcg cagaacccga
     9721 cgcacgtgta ccagacggct gccacctata cggtcaccct gaccgccacg aacggggccg
     9781 gttcggacag cgagacggca cagatcaccg tcacccaggc ggtcgcgccg gtggcgtcct
     9841 tcaccgcgac gccgtcgacg gggaacgcgc ccctctccgt gagtttcacg gacacgtcat
     9901 cggggaatcc gacagtatgg gcatgggact tcgagtcgaa cggcagcgtc gattcgaacg
     9961 cgcagaaccc gacccacatc tacacgacgc cggccacgta cacggcgacc ctgaccgtca
    10021 cgaacggtgc cggttcggac agcgagacgg cgcagatcgt cgtcaacccg ccgccgcagc
    10081 caacgaacct ggtgggcaac cccgggttcg atgtcgacac ggcgggctgg agtacggcgg
    10141 ggtccgggac gctggtcacg ctcacccgag tgacccccgg ccgcgacggc accggaggag
    10201 cggcccgcct gttcaacggt gcctccggca cgcggaagtg tgtgctgaac gatcagccga
    10261 actgggtcac gacgaccacg gccggcagat acacggcgag catctgggtt cgcggcgacg
    10321 tcgccggcgc cctgatcaag atcaacatca gggagatgaa cggttcgacc gtcgtgaagt
    10381 ccagggtggg gaacttcacc ctgacgaccg cgtggcagca gatcacggtc gcgcacacga
    10441 tcctctcgcc cggctcgacc ctcgatctcc aggtcttcct tccgaaggcg caggcccccc
    10501 cgggaacatg cttctacgcc gacgacgcat cgatcgtcag gtcctgatcc gcgcctcgcg
    10561 gcgggcctcg ggggactctg ttcgactcgg tacgtttcct acgtcgaagc gcgcatgcgc
    10621 cgagcccgtg agccccggca gactctccat ccaggaccag tcagagggag cgacatcgat
    10681 ggacaccccg gagtcctcga tcggctcgga tggaggggtc tgaaccgatg gccctccatg
    10741 agcctctcga gatcgcgttc gccccgacac cgccttcgct ccgctcccgg ccgccgttgt
    10801 cgagcctgct ccccgtgatg gacgccctga cgctgatcgg ggtgtcggcg atcctggggt
    10861 tccagctcgt ctcgatcgcc tacggggtgg ccacgttcct gatcttgaac ggcgattcct
    10921 cccgcaagta ccggatcaac ccgcggctgg gcgacgaagc gggctggctg ctcggccggg
    10981 tgtcggtcgc cctcctcgcc ctcctctcgg tcggatgggt cgcccagctc ttccacgtcg
    11041 acctgctggg gagcgagatc cgtgagctca cgaaggtcgg gctggccgtg gccggggcgg
    11101 tcttcctggg acgaggggcg gcctacgcat tgggtcggaa agcgaaggag cgaggcctgg
    11161 cgtcggagcg caccctgatc gtcggcgacg gggagctcgc catggggctg gccgagacgt
    11221 tcctggagca tcccgaatac ggcctgcggc cgatcggctt cctcgggcgg cccctcgtcc
    11281 gccacgtggc gttgccacag cttggggagg tccgcaacgt cgagcaggtg atcggcgact
    11341 tcgaggtgcg gcgcgtgctg gtggcgttcg gcgagatgcg tgatcaggag ctcgtgacga
    11401 tcctccgggc gagcgagcgg gtgccggcgg agatccacgt ggtcccgcgc ttcttcgagc
    11461 tgagcggcgt tccgcagggg gctcccgtgg acgacgtcgc cggcatcccc ctgttccacc
    11521 tgcggcgccc cgccctccgt ccgggcgctc gtttccagaa gcgagccttc gacatggtgg
    11581 tcgcatccat gctgttcctc ctgagcctgc cggtgttcgc ggtcatcgcg ctggcggttc
    11641 gcctgtcgag cgagggtccg gtcctgttcc gtcagaagcg ggtgggcctg aacggaaacc
    11701 tcttcgagat catcaagttc aggacgatgt acgtgaacga cgactcggac accgagtgga
    11761 tcacggacag ggatcgcgat cgtgtcaccc ccatcgggca tgtccttcgg gcggcgagcc
    11821 tggacgagct cccgcagctc atcaacgtga tccgagggga gatgtccttg gtgggtcccc
    11881 ggccggagcg accccactac accggccgat tctccgagac cgtctcccgc tacgacgatc
    11941 ggcatcgcgt cgtcggcggc atcaccgggt gggcgcagat caacggtcgg agcagagggc
    12001 tcgactcggt tccggagcgc gcacgcctcg acaactacta catcgagaac tggtccctct
    12061 ggcgggatct cgtgatcgtg gcccggacgg tcgcggtcct gttccggggc cgctagctca
    12121 ggccgtgccg cccggcggct ggtggccgcg ggccatcagc gcctcgtcga tccgggtgga
    12181 gccgaactcc tcgatgagcc gtcgcaggtc gcggcgcatc ctgccacggc cggtgtaggc
    12241 gcgcagacgg agcgatcgcg ccatctgtgg gagacgctgc tcctccgcat ccagctccca
    12301 ggggtggagg tagaaggtga accaggacgt ggagcgcaac ctcctggcga cgccgcggcg
    12361 gaacaccggg aacgggatca atcggaaata cgcgcccccg gcccacggca cccgtacgga
    12421 cccgaccgga a
//
LOCUS       DASUVE010000186         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_659397, whole genome shotgun sequence.
ACCESSION   DASUVE010000186 DASUVE010000000
VERSION     DASUVE010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659397"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..518
                     /locus_tag="VF036_04015"
     CDS             138..518
                     /locus_tag="VF036_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6264430.1"
                     /translation="MNPVLEVEVASRDDTTRIILRGGLDMATADDLTATVVPWLDDRP
                     ELRSIVIDVTDLTFMDSSGIKAIYDLTTPPRDLAVTLVSPTRAVRRVLDITGLVPADA
                     DTSDEVLITHLDGAGVVPTRLGWA"
     gene            567..932
                     /locus_tag="VF036_04020"
     CDS             567..932
                     /locus_tag="VF036_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6264431.1"
                     /translation="MREGLGTFDVIRSTDGSSDWCLVGELDLATAGDLNTVVLPWLEE
                     DENTGPIAFDVSELTFIDSTGLRTLYALAGPPHNRKLTLREPTDAVWQVLAILGFVSA
                     DGRRPAEITIRHDGSWEPI"
     gene            1104..1406
                     /locus_tag="VF036_04025"
     CDS             1104..1406
                     /locus_tag="VF036_04025"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6264432.1"
                     /translation="MSTIFSVTRISPSEFHLHGELDIGTAAIFEEEVTAVMPESGPVT
                     LDLSGLTFLDSSGTHAIHDAAAALDRGSVRLVGAHGAARRVIEISGFADAPGIEIA"
     gene            complement(1465..1776)
                     /locus_tag="VF036_04030"
     CDS             complement(1465..1776)
                     /locus_tag="VF036_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264433.1"
                     /translation="MSTRLRSSLTVAVLVLVVVAMGAPMAFAGEVTGSGKKQDQNQGR
                     SWCSFSGLNDRIEGEGPVEPRAQSFGIEVKHGHANPHDANPGFFCNPNRTPLPPNPNR
                     R"
     gene            1965..2372
                     /locus_tag="VF036_04035"
     CDS             1965..2372
                     /locus_tag="VF036_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEX6264434.1"
                     /translation="MGFGDAIRTCLSKYADFHGRAGRPEFWWFFLATWLIGTVIYLLA
                     GAADSAFLWLIGGVISLALLLPYLAVLVRRLHDTGRSGWWYFISLIPLVGFIILVVFL
                     ATEGQPGQNEYGPPPGGAAMGEATPPPPPPSPA"
BASE COUNT          381 a          901 c          846 g          412 t
ORIGIN      
        1 acctgatcgg gtcggacccc ggcccctcgg cccgacgacc tcggaccccg acccctggcc
       61 cgagcagcct cggaccccaa ccacctggtc accgctcgag tggcgtgcgt cgccgctccg
      121 acaggttggg ggtagctatg aacccggtcc tggaagttga agtcgcatct cgtgacgaca
      181 cgacgaggat catcctccgg ggggggctcg acatggcgac cgcggatgat ctcaccgcga
      241 ccgtcgtgcc ctggttggac gaccgcccgg agcttcggtc gatcgtcatc gacgtgaccg
      301 acctcacgtt catggacagc tccggcatca aggccatcta cgatctcacg accccaccgc
      361 gggacctcgc ggtcaccctc gtcagcccga cgcgggccgt ccgacgggtg ttggacatca
      421 cgggtctcgt gcccgcggac gccgacacct cggacgaggt gctcatcaca cacctcgacg
      481 gcgccggtgt cgtcccgacg cgtctggggt gggcgtgatc ttcgcgctgc gtgggtacgc
      541 cttccctgcg gaggagagga ccggcgatga gggaggggtt ggggacgttc gatgtgatca
      601 ggtcgaccga tggatcctcg gactggtgcc tcgtcgggga actcgacctc gcgaccgcag
      661 gtgatctgaa caccgtcgtc ctgccctggc tcgaggagga tgagaacacc ggcccgatcg
      721 cgttcgacgt gtcggagctg accttcatcg atagcacggg cctgcggacc ctctacgcgc
      781 tcgcgggtcc tccgcacaac cgcaagctca ccctgcggga accgacggac gccgtatggc
      841 aggtgctcgc gatcctcggg ttcgtctcgg cggatggccg ccggcccgcc gagatcacca
      901 tccgacacga cggcagctgg gagcccatct gagctgccgc atcgctcgac aacccatgca
      961 ctccggagag atcgtgcgcg agagcgcttc cgcaggttga ccttccggag ctcagatgcg
     1021 acggtgggtg tccacgtcgc ctggaccgtc cgcgcacgcc tgaccggtgg ccctcccgac
     1081 cctctggaag agaggccgcc cccgtgagca cgatcttctc ggtgacacgc atctccccga
     1141 gcgagttcca cctgcacggc gagctcgaca tcgggaccgc cgcgatcttc gaggaagagg
     1201 tcaccgccgt gatgccggag tcggggccgg tcacgctgga tctgtccgga ctgaccttcc
     1261 tcgacagctc cggcacccac gcgatccacg acgccgccgc cgcgctggac cgcgggagcg
     1321 tgcggctcgt cggggcacac ggagccgcac gccgggtgat cgagatctcc gggttcgccg
     1381 acgcccccgg catcgagatc gcctagcagc cccggcgcgt ggtgaccccc gctgagcggg
     1441 ggccaccacg cagcacgccg agcgctacct gcggttcggg ttcggcggca acggggtccg
     1501 gttcgggttg cagaagaacc ccgggttcgc gtcgtgcggg ttcgcgtggc cgtgcttgac
     1561 ctcgatcccg aacgactgcg cccgaggctc caccggtccc tcgccctcga tccggtcgtt
     1621 cagccctgag aacgagcacc acgatcggcc ctggttctgg tcctgcttct tgccgctgcc
     1681 ggtgacctca ccggcgaacg ccatcggcgc acccatggcg accaccacga gtacgaggac
     1741 ggcgacggtc agagatgacc gaagtcgtgt ggacatgctt cccctcccac tgcccggggg
     1801 gatccgacac cgtgacggat cggcagacag tcagcccgtt tgtaccggga cagcgtccga
     1861 gaggtcaagg ggggcaacgt cggccggatc tcccgcatgg aggtcgttgc tcaggggaca
     1921 ccgctcgcct aacgtccggc gtgtccaccc gaggagggaa ccagatgggg ttcggcgacg
     1981 ccatacggac gtgcctgtcg aagtacgcgg acttccatgg acgcgcggga cgtcccgagt
     2041 tctggtggtt cttcctggcc acctggctca tcgggacggt gatctacctg ctcgcgggtg
     2101 cggccgactc ggccttcctc tggctgatcg gcggggtcat ctccctcgcg ctgctcctgc
     2161 cctacctcgc cgtgctcgtg cggcgtctcc acgacacggg ccggtcggga tggtggtact
     2221 tcatctcgct catcccattg gtcgggttca tcatcctggt cgtgttcctg gcgaccgagg
     2281 gccagccggg tcagaacgag tacggtccgc cgccgggtgg cgcggcgatg ggtgaggcga
     2341 ccccgccccc gccgccaccc tcccccgcgt aggcgggctc agagcttcgg atagacgtac
     2401 ccgtacccct tcacgcgcag ggccttggtc gacgtcgagt acatccgccg tcgcgggatg
     2461 tcgcaacgcg cgccgcaccg accgcacgtg aaggcgacct gccagcgcgc cgggatcgcc
     2521 tggtgatagc cgcagccggg
//
LOCUS       DASUVE010000187         4751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1413473, whole genome shotgun sequence.
ACCESSION   DASUVE010000187 DASUVE010000000
VERSION     DASUVE010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413473"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2342
                     /locus_tag="VF036_04040"
     CDS             <1..2342
                     /locus_tag="VF036_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6264435.1"
                     /translation="TGPTWTFTTITGADPVLVGVGDIAKCDSLGDEATGDIVEGISGT
                     VFTAGDNVYQDGTASEFANCYDPSWGSFKGRTRPTPGNHDWNTGNLNGYNGYYGSNAT
                     DAGGKSYYGYDVGDDWHVTVLDTECAQPAVGGCGVDSPQEIWLRGDLAAHTDDNVIVI
                     WHKPRFSSGSTNLTSLQTFYEVIYAAGVDILLVGHDHVYERFAPSDPAGEIDTTYGVR
                     QFTVGMGGADHHPFGTIRSTSQVRNNDTFGVMKFTLHANSYDWKFLPVDGATFTDSGT
                     APTHDAPNGSPVIDSAAIDQAAPTTGQTLTVSVTSHDPDADPVTYAYAWQKDTGTGFQ
                     PIAGETGSTLDLSTAGNGSRGDRIRVVVTPNDGSVDGAPVTTSSVTIANTAPVATVSL
                     DDPTPSTNAVLTATATGSDADADAVSFTYVWRVDGAIVKTTAGTSSLTDTLDLSQAGN
                     GDTGQTVSVTVTPNDGTLAGTSVGDSAVVQAALPATPSGVVALVSPTSIELDWANNPP
                     TDLAGYNVYRSGAAAGTYVKLNNVLLTTSAFSDTAAPVGSPSFYRITAVNTSAQESTP
                     ASTSGTRKIGFRGAATASQKGAVGLTISAPTGIQPGDVLIAAVNVRGAPTVTSPGTGW
                     TLIRDTGNGNTFHELTYWKVVAVGDPTSYRWTFSSSQVAAGAILGYRGVDVNAPIEIH
                     DASTATNSKQITAPAVTSTYQGGLVLGLFGLAQQAAITPPLAMFERAEIVAAGKSKIV
                     CEVADSILQAAGLTGTRTALANKAGANIGQVIVLRPAIG"
     gene            complement(2391..3272)
                     /locus_tag="VF036_04045"
     CDS             complement(2391..3272)
                     /locus_tag="VF036_04045"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6264436.1"
                     /translation="MLLTSRAEYQHEGSITAAFGGLYMERTLERIDRRQADSLAAMVT
                     SIRSGEPLAPVVEGFLAEGMSAEEAASLERAAHELVRVDNLFGSYVGPQLAARLHEEP
                     EFGELGGREADVTALFADLVGFTAFSEGKSASDVIEMLNRYWEGVVPVVVEAEGGVIE
                     RFAGDAVMAVFNALDDQPDHALRGARAALAMQAFTQVTATEHPDWPRFRIGVNTGTAV
                     IGTVGATDQRSFAAIGDTTNVAARVQALARPGQVLLADATYQRMRDRVRVEPLGPTTL
                     KGRTETIDVYELLGVVS"
     gene            3536..3898
                     /locus_tag="VF036_04050"
     CDS             3536..3898
                     /locus_tag="VF036_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264437.1"
                     /translation="MRDAEAQATKTAMRAHRSHRIVERESVPGPRSGCIAEAAANPAA
                     RNSPIGVAITNPLFGSFTMASGVASACNARNPALARSAIDTRNSRASSRRVAASLIAI
                     PSTVSMKATLRTSQKWEG"
     gene            complement(4123..4296)
                     /locus_tag="VF036_04055"
     CDS             complement(4123..4296)
                     /locus_tag="VF036_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264438.1"
                     /translation="MLFLVGAIALALVALGTSWGTIGTVVILITATAWLTLAFRPVEA
                     GALVTNLTRRRFE"
     gene            4522..4592
                     /locus_tag="VF036_04060"
     tRNA            4522..4592
                     /locus_tag="VF036_04060"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4554..4556,aa:Cys,seq:gca)
     gene            4617..4688
                     /locus_tag="VF036_04065"
     tRNA            4617..4688
                     /locus_tag="VF036_04065"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4649..4651,aa:Val,seq:gac)
BASE COUNT          856 a         1611 c         1575 g          709 t
ORIGIN      
        1 ccacgggccc cacgtggaca ttcacgacga tcaccggtgc cgaccccgtt ctcgtcggcg
       61 tgggtgacat cgcgaagtgc gactcgctcg gggacgaagc aacgggggac atcgtcgaag
      121 gcatcagtgg aacggtgttc acggcgggag acaacgtcta ccaggacgga accgcatcgg
      181 agttcgcgaa ctgctatgac ccgtcctggg gatcgttcaa gggccggacc cgtccaacgc
      241 ccggcaacca cgactggaac accggcaacc tgaacgggta caacggctac tacggctcga
      301 acgcgacgga cgctggcggc aagagctact acggctacga cgtgggcgat gactggcacg
      361 tgaccgtcct ggacaccgag tgcgcacagc ctgcagtcgg cgggtgcggc gtcgattcgc
      421 cccaggagat ctggctacgc ggagatctcg cggcccatac ggacgacaac gtgatcgtga
      481 tctggcacaa gccacggttc agctcaggat cgaccaatct cacctccttg cagacgttct
      541 acgaggtcat ctatgcggca ggagtggaca tcctcctcgt ggggcacgat cacgtctacg
      601 agcgattcgc tccgagtgat ccggccggcg agatcgatac gacgtatggc gttcgacagt
      661 tcacggtcgg tatggggggt gcggaccacc acccgttcgg gacgatccgg tccactagcc
      721 aggttcgcaa caacgacacc ttcggcgtga tgaagttcac gctgcacgcg aacagttacg
      781 actggaagtt ccttcctgtg gacggtgcga cgttcacgga ttcagggacc gctccaaccc
      841 acgacgcgcc gaacggctct ccggtgatcg actcggccgc gatcgaccag gccgcgccca
      901 ccaccggtca gacactcacg gtgagcgtca cgtcgcacga ccccgacgcc gatccggtca
      961 cctacgccta cgcgtggcag aaggacaccg gcaccggctt ccagccgatc gccggtgaga
     1021 ccggctccac cctcgatctg tccacggccg gcaacggtag ccgcggcgac cggatccgtg
     1081 tggtggtcac accgaacgac ggctccgtgg acggcgcgcc cgtcacgacg agctcggtga
     1141 cgatcgcgaa caccgcaccg gtggcgaccg tctcactcga tgatccgacc ccttcgacga
     1201 acgcggtcct caccgcgacg gcgaccggct cggacgccga tgccgacgcc gtgagcttca
     1261 cctacgtgtg gagggtcgac ggcgcgatcg tgaagaccac cgcggggacc agcagcctca
     1321 ccgacaccct ggacctctcg caggcgggca acggtgacac cggccagacg gtgagcgtca
     1381 ccgtcacacc gaacgacggc acgctcgcgg gcacctccgt cggggactcc gccgtcgtcc
     1441 aggcggcgct gcctgccacg ccgtcgggag tcgtcgccct cgtctcgccg acgtccatcg
     1501 agctcgactg ggcgaacaat cctccgacgg acctggcggg gtacaacgtc taccggagcg
     1561 gagctgccgc tggaacatac gtgaagctga acaacgtgct actcaccacg agcgcgttct
     1621 cagatacggc cgcgccggtc ggctccccga gcttctaccg gatcacagcg gtgaacacga
     1681 gtgcgcaaga atcgacgcct gcctccacct cggggacgag gaagatcggc ttcaggggcg
     1741 cggcgacggc gtcgcagaag ggtgccgtag ggctgacgat cagcgcaccg acagggatcc
     1801 agccgggtga cgtgctgatc gcggcggtca acgtgcgcgg tgcgccgacc gtgacatccc
     1861 cagggacggg ctggacgctg atccgcgaca ccgggaacgg caacaccttc cacgagctca
     1921 cgtactggaa ggtcgtggca gtgggagacc cgacctccta tcggtggacg ttctcctcat
     1981 cgcaggtcgc tgcaggcgcg atcctcggct acagaggggt cgacgtgaac gcaccgatag
     2041 agatccatga cgcctcgacc gctacgaact cgaagcagat caccgctccg gccgttacga
     2101 gcacgtatca agggggcctc gtgctgggac tcttcgggct ggctcagcag gcggcgatca
     2161 cgcctcccct ggcgatgttc gagagagccg agatcgtggc tgccgggaag agcaagatcg
     2221 tctgcgaggt cgcggattcg atcctgcagg cggcgggcct gaccggtacc cggaccgcgc
     2281 tggcgaacaa ggccggcgcg aacatcggac aggtcatcgt gttgcggcca gcgatcggct
     2341 gatctcggag gtgggggagg ccggcctatc gggccggtct cctgcgtctg tcaggaaacc
     2401 accccgagca gttcgtagac gtcgatggtc tccgtcctgc ccttcaacgt cgtgggaccg
     2461 agcggttcga cacgcacgcg atcgcgcatc cgctggtagg tggcgtccgc gaggagcacc
     2521 tgtcccgggc gggcgagcgc ctgcacccgc gccgcgacgt tcgtcgtgtc accgatggcg
     2581 gcgaagctcc gctgatccgt cgcgccgacg gtcccgatca cggccgtgcc ggtgttcaca
     2641 ccgatccgga agcgaggcca gtccggatgc tccgtcgcgg tcacctgcgt gaacgcctgc
     2701 atcgcgagcg cagccctggc tccccggagg gcgtggtccg gctggtcatc gagcgcgttg
     2761 aacaccgcca tcacggcgtc cccggcgaac cgctcgatca cgccgccttc ggcctccacg
     2821 accaccggga cgaccccctc ccagtatcga ttgagcatct cgatcacgtc cgatgcggac
     2881 ttcccttcac tgaacgcggt gaacccgacg agatccgcga acagcgcggt gacgtcggct
     2941 tcccggcccc cgagctcgcc gaactcgggc tcctcgtgca aacgtgccgc gagctgcggg
     3001 ccgacgtagg agccgaagag gttgtcgacg cggacgagct cgtgggcggc gcgctcgaga
     3061 gacgcagcct cctcggcgct catcccctcg gcgaggaagc cctccacgac gggcgcgagt
     3121 ggctcccccg accggatcga tgtcaccatc gcggcgagtg aatcggcctg ccgacgatcg
     3181 atgcgttcga gcgtccgctc catgtagaga cccccgaagg ccgccgtgat cgatccctcg
     3241 tgctggtact cggcccggga ggtcagcagc acgatgagga acggcgaccg ccatcagcac
     3301 gtgccattcc caccaggatg cgtgccaact gcgagcgacg atgatcgtga ggacggcctc
     3361 ggcaaggagg atgaacgcag tggcaacggc gagcgggagc ggtcgtcgcc tgcgggcgta
     3421 gacggccagg tagcgtgccg ccgcgaacac gtacagcccg atcgcgaccg gcgcgaggat
     3481 ccgaaggagt gtgggagcct cgctgggggc aggcccgcga agcaacgaga caccgatgag
     3541 ggacgcggaa gcccaggcca ccaagaccgc gatgagggcc caccggagcc atcgcatcgt
     3601 ggagcgtgag agcgtccccg gtccgcgctc cggttgcatc gcggaggcgg ccgcgaaccc
     3661 ggccgcgagg aacagcccga tcggggtcgc gatcacgaac ccgttgttcg gctccttcac
     3721 gatggcctcg ggcgtcgcga gcgcgtgcaa cgcgaggaat ccggcactcg cgaggagcgc
     3781 catcgatacg aggaacagcc gagcatcgtc ccggcgggtg gcagcctcgc tcatcgcgat
     3841 cccgagcacc gtgtcgatga aggcgacgct gaggacgagc cagaaatggg aggggtgatg
     3901 ctcccagtgc acgtccagat cggggaccgc gaggagcagt gcgagtcccg cgagcggcaa
     3961 cgagagcgcc gccgcgagca cgatcgcctt gctccgggag ctctccaccc ctgcatcctc
     4021 cccgcgagcc gatcggacct caactcgatg cggagaaatc agcgatgtcc ggacgcgacg
     4081 aaggccggcc cttcggaccg gccccgtgcg gatccctgct ggttactcga agcgccggcg
     4141 tgtcaggttc gtgaccaacg cccccgcctc gaccgggcgg aacgcaagcg tcagccatgc
     4201 cgtggcggtg atcaagatca cgacggtgcc gatcgttccc cagctcgtgc cgagggcgac
     4261 gagcgccagt gcgatggctc cgaccaagaa cagcatcgtc ctttcctttc tcgattccgc
     4321 gtcgtaacgc ggaggttgcc ctaccttgct cttcgaggct accggcggag ggatcctgtg
     4381 aggccggtgc cgcgtgaaca ctgtgtgaac accctccagg cggtcggatg ttcccgtcgg
     4441 gccgatctcc ggcgtttctg taccctgcac ccttcgaggg gccagcctgt cgcgagccgc
     4501 ggctagactg acccctcgtc cggcggggtg gccgagtggt taggcagagg cctgcaaagc
     4561 ctcgcacacc ggttcgattc cggtccccgc ctcgaggccc gctacgatga gggctcgggc
     4621 gcttagctca gggggagagc gcttccttga cacggaagag gtcagaggtt caaatcctct
     4681 agcgcccacc gataaacatc cagctcagag cacctatcgc cctgtccggg gttcgtgcta
     4741 gattcctcgt g
//
LOCUS       DASUVE010000188         5962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_94527, whole genome shotgun sequence.
ACCESSION   DASUVE010000188 DASUVE010000000
VERSION     DASUVE010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94527"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="VF036_04070"
     CDS             complement(<1..157)
                     /locus_tag="VF036_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020025844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX6264439.1"
                     /translation="MCGIAGIIHRDGSADVGTEMTRMLQAMKHRGPDSAGYALFGPPS
                     ELVVIRFK"
     gene            complement(180..1577)
                     /locus_tag="VF036_04075"
     CDS             complement(180..1577)
                     /locus_tag="VF036_04075"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017299852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX6264440.1"
                     /translation="MATDIEQFVDAPGRDAAIKEVRRAIDAEGVTYMYYQFVSVTGRI
                     MGKGIPAKHWETIARKGFQLVYGSTANLFVDRHGDYIGYGPEARELVGIPDVDTFCIL
                     PWDRRTARVFCTLFRGREEEVDGGAFLTSDCRGNLKRIHDEFTAETGLHLRAGTEPEM
                     MWLKTNPDGTPSVDGKTKPYCYHIDQFSELQPIIHKVMEYGEAMGLDMIQGDHEDAPG
                     QLELNFMFDKAEATADRLTTYRQICRQVGREMNAFACFMPKPFMGVSANGCHHNISLW
                     QGDENMFMPDGDDPRLPGKIGLHAIGGVLEHVRALTCITSPTVNSYRRFADAGFWAPI
                     FADWGFQNRTTALRISAPGRYEYRSVDSAVNPYLSFAALLMAMQDGLARNLDPGPPEE
                     RNIYEAIAQGKDVARIPVTLGEALDALEEDEIVKSALPGDMYRVFMHYKRDEWERFIG
                     TVSDWDIAEYLDVLP"
     gene            1739..3409
                     /locus_tag="VF036_04080"
     CDS             1739..3409
                     /locus_tag="VF036_04080"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX6264441.1"
                     /translation="MKSGLEIAQEAVLRPIIDVAADAGILEDELEQYGRSKAKVSLSI
                     LDRLADRPDGKLVITTAITPTKAGEGKTTTSISLTQGMGRIGKDVMLCLREPSMGPVF
                     GIKGGGNGGGYAQVVPMEEINLHFNGDFHAVQAAHNLLAAALDASIYHGNPLGIDPVS
                     VTWPRTLDVNDRELRYSVVGLGGKAHGVPRENQFVIVAASEIMAVLALSSDLADLRTR
                     LGRIVVASTYDGTPVTADELRVAGAMAVIMKEAIKPNLVQTLEGQPTLIHAGPFGNIA
                     HANNSIIEDRIALKLADYVVTEAGFASDLGFQKFCDIVCRFGGFAPAAGVLVTTVRAT
                     KSHGGMPFDQLGTEDLDALRRGIDNLEAHIGIVREYGLPCVVSINAFPTDTEKEIETI
                     RELAGGAGAEKVVVNRAFAEGGAGATELAEAVVAACERPSTFHQLTPDGAPIKEQIEA
                     IATKIYGADGVDYQAQAEKDVARMEQLGFGAMPVCMAKTHLSLSHDPQLLNRPKGYRL
                     PVRGVVPSAGAGFVVVLCGDMQRMPGLGRTPAFMQVDLDPDGRIVGLF"
     gene            complement(3472..3834)
                     /locus_tag="VF036_04085"
     CDS             complement(3472..3834)
                     /locus_tag="VF036_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6264442.1"
                     /translation="MPDERVSAILKTADVARTIEWYREAGFELRGVHPETGEPTWCEL
                     SRDGVVLQFLGGETPWPDPPGFTGTLYFYPRSVEALAKEIEGRIEPAWGPEVREWGSR
                     ELGLRDPSGYFLTFTEDA"
     gene            3943..4728
                     /locus_tag="VF036_04090"
     CDS             3943..4728
                     /locus_tag="VF036_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264443.1"
                     /translation="MSFTATASAPSRTRGDVMAARLRTAAIVAIGSGIAGALVGGLGS
                     RLAMRLVALAAPEVRGAVTENGAIVGEITLAGTVVLVVFAGVASTALGAGVFVVVKPW
                     LPERTVPRGIVFGGFLLATMGSSVVDAGNADFVLLGDRLLNVAIFSALFLAFGVVASA
                     SFTALEARVPEAAAFSPRMWAVSAVCALPLIPGVIGMAVGFGPKLGVPLLAASAAMLA
                     VPSVERRGHQGRTRPIRIGATAGMLVVVGLAGADYIDNVITIL"
     gene            complement(4735..5166)
                     /locus_tag="VF036_04095"
     CDS             complement(4735..5166)
                     /locus_tag="VF036_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016337385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6264444.1"
                     /translation="MRIESVVFDCHDAAPLARFWADALGWSVAPYDEEELERLASKGI
                     YDPEDDPSVMVQPPAGTDLPVLFFTEVPEEKATKNRVHLDLGADGPLEGEVQRLEELG
                     AHVRNWAEDDGSTWCVMLDPEGNEFCVVTASDEDDDEEEED"
     gene            complement(5214..5846)
                     /locus_tag="VF036_04100"
     CDS             complement(5214..5846)
                     /locus_tag="VF036_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEX6264445.1"
                     /translation="MGTRDGMIGSWLDELGSASPTPGGGAAAGVAASMGAALIAMVGH
                     LTVGKEGFEDLDARMRTLVETAEDEGKAFLALADEDAAAFESVMAAFRLPKDTDEEKA
                     SRTMRIQEAYAGAAAVPLELATRCVRLMDLAEDATAMGNPHASSDGYSAGFLLFAAAL
                     AAMANVRINAAGLKDEATGQGLVDECDALRARADALLQQLEEAFLLRLSA"
BASE COUNT          901 a         2069 c         2000 g          992 t
ORIGIN      
        1 gtttgaatcg gatcaccacg agctccgagg gcggtccgaa cagcgcgtag ccggcggagt
       61 ccggaccacg gtgcttcatc gcctggagca tccgggtcat ctcggtcccc acgtccgcgg
      121 agccgtcccg gtggatgatg cctgcgatcc cgcacatgtg aagaagagag ctcctttcgt
      181 cagggaagca cgtcgaggta ctcggcgata tcccagtcgc tgaccgttcc gatgaatcgc
      241 tcccactcgt cgcgcttgta gtgcatgaac acccggtaca tgtccccggg cagcgcggac
      301 ttcacgatct cgtcctcttc gagtgcatcc agggcctcgc cgagggtcac cgggatcctc
      361 gcgacgtcct tcccctgggc gatcgcctcg tagatgttcc gctcctcagg agggcccggg
      421 tctaggttgc gggccagtcc gtcctgcatc gccatcagga gcgccgcgaa ggacaagtac
      481 gggttcacgg ccgaatccac ggaccggtac tcgtaccgcc cgggcgcgga gatccgcagc
      541 gcagtcgtcc ggttctggaa cccccagtcg gcgaagatcg gcgcccagaa ccccgcgtcg
      601 gcgaatcgtc ggtaggagtt caccgtcggt gaggtgatgc aggtgagcgc ccggacatgc
      661 tcgagcactc caccgatcgc gtgcagaccg atcttcccgg ggagccgggg gtcgtcgccg
      721 tcgggcatga acatgttctc gtcgccctgc cacagcgaga tgttgtggtg gcaaccgttg
      781 gcggagacgc ccatgaacgg tttgggcatg aagcaggcga acgcgttcat ctcccgcccg
      841 acctgacggc agatctgacg gtaggtcgtc aggcggtcgg ccgtggcctc tgccttgtcg
      901 aacatgaagt tcagctcgag ctgtccgggg gcgtcctcgt gatcgccctg gatcatgtcg
      961 aggcccatcg cttccccgta ctccatcacc ttgtggatga tcggttggag ctccgagaac
     1021 tgatcgatgt ggtagcagta cggtttggtc ttgccatcca ccgacggcgt gccgtccggg
     1081 ttcgtcttga gccacatcat ctcgggctcg gtgcccgccc gcaggtgcag tcccgtctcg
     1141 gccgtgaact cgtcgtggat ccgcttcagg ttccctcggc aatccgacgt gaggaacgcg
     1201 ccgccatcca cctcttcctc tcgcccgcgg aacagcgtgc agaagacgcg ggccgtcctc
     1261 cgatcccacg gcaggatgca gaaggtgtcg acgtcgggga tccccaccag ctcccgagcc
     1321 tcgggcccat agccgatgta gtcgccgtgc cgatcgacga acaggttcgc ggtcgaaccg
     1381 tagacgagct ggaaaccctt ccgagcgatc gtttcccaat gcttggcggg gatccccttg
     1441 cccatgatgc ggccggtgac cgagacgaac tggtagtaca tgtacgtcac gccttcggcg
     1501 tcgatcgccc ggcggacctc cttgatcgcg gcgtcccggc ccggtgcgtc cacgaactgc
     1561 tcgatgtcgg tggccaactg acgcctccct tcggcggccc taccacgggc cagcttggcg
     1621 catcctacgt cgagtccccc ggcctgcgcc atgtgccggg ccacggtccg cggctcgcag
     1681 gtgaggggta tcctcacgca gccccggagc agccggcgga accgagggag gcgaggccgt
     1741 gaagagcggg ctggagatcg cgcaggaggc cgtccttcgg cccatcatcg acgtggcggc
     1801 ggacgccggg atcctcgagg acgagctcga gcagtacggc cgatcgaagg cgaaggtctc
     1861 gctctcgatc ctcgatcgcc tggccgaccg gcccgacggc aagctcgtca tcacgaccgc
     1921 gatcacaccg acgaaggccg gcgaaggcaa gacaaccacg tcgatctcgc tcacccaggg
     1981 catgggtcgg atcggcaagg acgtgatgct ctgcctccgt gagccgtcga tgggccccgt
     2041 cttcgggatc aaggggggcg gcaacggagg cggctacgct caggtcgtcc cgatggagga
     2101 gatcaacctc cacttcaacg gcgacttcca cgccgtgcag gccgcgcaca acctgctcgc
     2161 ggccgccctc gacgcctcga tctaccacgg gaacccactg ggtatcgatc ccgtctcggt
     2221 gacgtggcca cgaacgctgg acgtgaacga tcgcgaactt cgctattcgg tcgtcggcct
     2281 gggcgggaag gcacacggcg tcccgcgcga gaaccagttc gtgatcgtgg cggcctcgga
     2341 gatcatggcc gtgctcgcgc tgtcgagcga tctcgccgac ctgcggacga ggctcggtcg
     2401 gatcgtggtg gcctccacct acgacgggac accggtcacg gccgacgagc tgcgtgtggc
     2461 cggggcgatg gcggtgatca tgaaggaggc gatcaagccg aacctcgtcc agacgctcga
     2521 ggggcaaccc acgctgatcc acgcggggcc cttcgggaac atcgcacacg ccaacaactc
     2581 gatcatcgag gatcgcatcg ccctgaagct ggccgactac gtcgtgaccg aggcgggctt
     2641 cgcctcggac ctggggttcc agaagttctg cgacatcgtg tgccggttcg gcggcttcgc
     2701 cccggccgcc ggtgtcctgg tcacgaccgt ccgcgccacg aagtcccacg gcggcatgcc
     2761 gttcgaccag ctcggcaccg aagacctcga cgcgcttcgc cgtgggatcg acaatctcga
     2821 ggcgcacatc ggcatcgtcc gggagtacgg gctcccgtgc gtggtctcga tcaacgcctt
     2881 cccgaccgac accgagaagg agatcgagac gatccgggag ctcgcgggcg gagccggcgc
     2941 ggagaaggtg gtggtgaacc gggcgttcgc cgagggcggc gccggcgcta cggagctcgc
     3001 ggaggccgtc gtcgcggcct gcgagcgacc gagcacgttc caccagctca cgcccgacgg
     3061 tgcaccgatc aaggagcaga tcgaggcgat cgcgacgaag atctacggcg ccgatggggt
     3121 cgactaccag gcccaggccg agaaggacgt cgcccggatg gaacagctcg gtttcggagc
     3181 gatgcccgtc tgcatggcca agacccacct ctcgctcagc cacgaccccc agctcctgaa
     3241 tcgaccgaag gggtaccggc tgcccgtccg cggcgtggtg ccgagcgccg gcgcgggctt
     3301 cgtcgtcgtg ctctgcggcg atatgcaacg gatgccgggc ctgggccgga ccccggcgtt
     3361 catgcaggtc gacctcgatc ccgacggtcg gatcgtcggc ctcttctagg cgactccggc
     3421 gcgttccggc tcggtcttcg ggctgggggg cgccgctcct tcgaggaacg gtcaggcatc
     3481 ctcggtgaac gtcaggaagt atccgctggg atcgcgcagc ccgagttccc gggatcccca
     3541 ctcacggacc tcgggtcccc aggcgggctc gatccggccc tcgatctcct tcgcgagggc
     3601 ctccacgctt cgaggataga agtacagcgt gcccgtgaag cccggtggat cgggccaggg
     3661 tgtctcaccg cccaggaact gcaggaccac accgtcgcgc gagagctcgc accaggtggg
     3721 ctcccccgtc tccgggtgga ccccgcggag ttcgaacccc gcctcgcggt accactcgat
     3781 cgtgcgtgcg acgtctgcgg tcttgaggat cgcggatact cgctcgtcgg gcacgcaggg
     3841 atgatacggc gaggtcgaga ccgcctgcgg gtggctgcat cccgcgggat ccgggtgtcc
     3901 gccacctggc tccgcccgga gcggcgcgcg acgatcctgc ccatgagttt caccgcaacc
     3961 gcctccgctc cctctcgaac ccgcggtgac gtgatggccg cacgcctgcg aacggcggcg
     4021 atcgtggcga tcggctcggg catcgcggga gcgctcgtcg gaggcctcgg gagccgtttg
     4081 gcgatgcggc tggtggcact ggcagctccg gaggttcgtg gggcggtcac cgagaacggc
     4141 gcgatcgtcg gcgagatcac gctggcgggg accgtcgtgc tggtggtctt cgcgggggtc
     4201 gcgtccacgg cgctcggagc gggtgtcttc gtcgtcgtga agccgtggct ccccgagcgg
     4261 accgtcccgc gcgggatcgt cttcggcggg ttcctgctcg cgacgatggg ctcctcggtg
     4321 gtcgacgccg gcaacgccga cttcgtgctc ctcggggatc gcctcctgaa cgtggcgatc
     4381 ttctccgccc tgttcctggc gttcggcgtg gtcgcctcgg cgtcgttcac ggccctggag
     4441 gcgcgggtgc cggaagccgc cgcgttctcg cctcggatgt gggcggtctc cgcggtctgc
     4501 gccctcccgc tgatccccgg cgtgatcggc atggcggtcg ggttcggacc gaagctcggc
     4561 gtcccgctcc tggccgcatc ggcggcgatg ctcgccgttc cctccgtcga gcgccgcggg
     4621 catcaggggc ggacgcggcc gatccgcatc ggcgcgacgg cgggcatgct cgtcgtcgtc
     4681 gggctcgccg gtgccgacta catcgacaac gtgatcacga tcctctaggt cccgtcagtc
     4741 ctcctcctcc tcgtcgtcgt cctcgtcgga cgcggtgacc acgcagaatt cgttgccctc
     4801 gggatcgagc atgacgcacc aggtgctccc gtcgtcctcg gcccagttcc gcacgtgggc
     4861 ccccagctcc tccaggcgct gtacctcgcc ctcgagcggc ccgtcggccc ccaggtccag
     4921 gtggacccga ttcttcgtgg ccttctcctc cggaacctcg gtgaagaaca gcacgggcag
     4981 atcggtgccc gcgggaggtt gcaccatcac ggacgggtca tcttcgggat cgtagatccc
     5041 cttcgacgcg aggcgttcga gctcctcctc gtcgtacggg gcgacggacc accccagcgc
     5101 atccgcccag aaccgggcga gcggcgcggc gtcgtggcaa tcgaagacga ccgattcgat
     5161 cctcaccgtc ttcgaagcct aggccaagcc gatggcgggc gctcgggtcg gagtcaggcg
     5221 gacagacgca ggaggaacgc ttcctcgagc tgctggagga gggcgtccgc gcgagctcgc
     5281 aacgcatcac actcgtcgac gagcccctga ccagtcgcct cgtccttgag tccggcggcg
     5341 ttgatccgga cgttcgccat ggcggcgagt gcggccgcga acaggaggaa acccgcggag
     5401 tagccatcgg aggacgcatg gggattcccc atcgcggtcg cgtcctctgc gaggtccatc
     5461 aaccggacgc agcgggtggc cagctcgagc gggaccgcgg cggcgcccgc gtacgcctcc
     5521 tggatccgca tggtccggga ggccttctcc tcatccgtgt ccttggggag ccggaaggcc
     5581 gccatcaccg actcgaacgc ggctgcgtcc tcgtcggcga gcgcgaggaa ggccttcccc
     5641 tcgtcctcgg ccgtctccac caaggtgcgc atacgcgcat ccagatcctc gaatccctcc
     5701 ttgccgacgg tgaggtgtcc caccatcgcg atcagagccg cccccatcga agcggcgacc
     5761 ccggcggcgg caccgccccc cggggtcggc gatgcagagc cgagctcgtc cagccacgat
     5821 ccgatcatgc cgtcgcgcgt ccccatcgag gtctcctctc ttccccaaga tcggcggtca
     5881 gccggtctcg acgagccgtt ccaggatctg cgtctccgcc gagaagccgg cgagtcgtac
     5941 gtgttccgac gttccctccg gc
//
LOCUS       DASUVE010000189         3111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1413827, whole genome shotgun sequence.
ACCESSION   DASUVE010000189 DASUVE010000000
VERSION     DASUVE010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413827"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /gene="rbfA"
                     /locus_tag="VF036_04105"
     CDS             complement(<1..343)
                     /gene="rbfA"
                     /locus_tag="VF036_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX6264446.1"
                     /translation="MTQGARTARVAEEFREVLAEEIQKLKDPRIGFVTVTGVQVTADL
                     RRAWVSYTVLGDDKQQRSTAAGLRSATPHLRQALGRQVRLRNLPELEFQVDQTLAQHQ
                     QIEEIIERLHRE"
     gene            complement(367..660)
                     /locus_tag="VF036_04110"
     CDS             complement(367..660)
                     /locus_tag="VF036_04110"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX6264447.1"
                     /translation="MLVAALRLDLRIPACGSLKEKRHVIKTLANGIRSTFQVAVAETG
                     YQDMWQRAELGVAAVGSEGHHLKKVMHGVERFVERWAEVDIIDIDLTLHSPED"
     gene            complement(660..2813)
                     /gene="infB"
                     /locus_tag="VF036_04115"
     CDS             complement(660..2813)
                     /gene="infB"
                     /locus_tag="VF036_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX6264448.1"
                     /translation="MRIHELAKELGVTSKELLETLEKMGVAGKSASSSVPEDLVPRLR
                     ASGGKVTKPAKPRQVMEPPPKPRPKAKPKPKATPKVVAVPDAERARTEEHEGAPEPTA
                     AAADVPATPAPLAAVPDGAAPAAPPAPPAGPILQVVRGSTPQIFAEKTNRSPADIVKI
                     LFMAGEMVTATTSLSDEALQTLGSELGYHVEIVGMEDELQAEELGDEVDESELVPRSP
                     VVTVMGHVDHGKTKLLDAIRTTDVVSGEFGGITQHIGAYQAHVNGREITFIDTPGHEA
                     FTAMRARGAQITDIAVLVVAADDGVMPQTVEALDHAKAAGVPVVVAVNKIDKEEADPN
                     RVRTQMVERGLVPSEWGGDTEFVDVSAKTSANLEGLLETVLIVADLAELKGDPTGRAR
                     GTVLEAHLDKGRGPVATVLVQKGTLEVGDSLVCGITHCKVRAMVDENGQPVDVAGPAK
                     PAQVLGWNGVPNVGDEFREVDDEREARHIASEREARVRSAEVVNQRPASLTELLRRQE
                     RQEIPELNLVVKADVQGSLGALTDAFLKLPQDEVRVNIVHSGAGAITEHDVDLAKTAA
                     AIVVGFNVRPNANARELAEKEGVDIRLYRVIYDAIDDVKAALSGMLKPEERESIVGEA
                     EVRQTFRVPRLGVIAGCYMTRGTIRRNSQVRLIRDGVVVYEGRVGSLRRFKDDVAEVR
                     EGFECGIGLENFQDVKEGDVIEAFEVEEVARQISS"
     gene            complement(2810..3079)
                     /locus_tag="VF036_04120"
     CDS             complement(2810..3079)
                     /locus_tag="VF036_04120"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEX6264449.1"
                     /translation="MAREPERSCVGCRKRAPKADLLRIVRSETGAQVDPIGSASGRGA
                     YVHRDPACADAAVRRGAVASALRSGLTQGELARLRNEIEEASQAT"
BASE COUNT          418 a         1139 c         1016 g          538 t
ORIGIN      
        1 cctcgcgatg cagacgctcg atgatctcct cgatctgctg atgctgcgcg agcgtctggt
       61 ccacttggaa ctccagttcc gggaggttcc ggagccggac ctgccgaccg agcgcctgcc
      121 ggaggtgcgg cgtcgccgac cggagccccg ccgccgtcga tcgctgctgc ttgtcgtccc
      181 cgaggacggt gtaggagacc cacgcccgcc gcaggtcggc cgtcacctgg acaccggtga
      241 ccgtgacgaa gccgatccgg gggtccttga gcttctggat ctcttcggcg aggacctcgc
      301 ggaactcctc ggccacccga gcggtccggg ctccctgcgt catctcagca tcccgccttc
      361 tctggctcag tcctcgggcg aatggagcgt caggtcgatg tcgatgatgt cgacctcggc
      421 ccaccgctcc acgaagcgct cgacgccgtg catcaccttc ttgaggtggt ggccctccga
      481 cccgacggcc gccacgccca gctccgcacg ctgccacatg tcctggtatc ccgtctcggc
      541 caccgccacc tggaaggtgg accggatccc gttcgcgagg gtcttgatca cgtgacgctt
      601 ctccttcaac gatccgcacg ccgggatccg gagatcgagc cggagggcgg cgaccagcat
      661 caggaggaga tctggcgggc gacctcctcg acctcgaagg cctcgatcac gtcgccctcc
      721 ttcacgtcct ggaaattctc gagcccgatg ccgcactcga atccctcgcg cacctcggcg
      781 acgtcgtcct tgaaccggcg gagcgatccc acgcggccct cgtagacgac gacgccgtcg
      841 cggatcaggc ggacctgcga gttccggcgg atcgtccctc gggtcatgta gcacccggcg
      901 atcactccga gccgcggcac gcggaacgtc tggcgaacct ccgcctcgcc gacgatcgac
      961 tcgcgttcct ccggcttgag catgcccgag agcgcggcct tcacgtcgtc gatcgcgtcg
     1021 tagatcaccc ggtagagccg gatatccacg ccttccttct cggcgagctc gcgcgcgttc
     1081 gcgttgggcc ggacgttgaa ccccacgacg atcgcggcgg ccgtcttggc gaggtcgaca
     1141 tcgtgctcgg tgatcgcgcc ggcgccggag tgcacgatgt tcacgcggac ctcgtcctgc
     1201 ggcagcttca ggaacgcgtc cgtcagcgct ccgagcgagc cctggacgtc ggccttcacc
     1261 acgaggttca actcggggat ctcctggcgc tcctggcgcc ggaggagctc cgtgagcgac
     1321 gcgggccgct ggttgaccac ctcggccgat cgaacgcgtg cttcgcgttc ggaagcgatg
     1381 tgacgcgcct cgcgttcgtc gtcgacctcc cggaactcgt ccccgacgtt ggggacgccg
     1441 ttccagccga gcacctgcgc gggcttcgcg ggcccggcca cgtcgacggg ctgcccgttc
     1501 tcgtcgacca tcgcccggac cttgcagtgc gtgatgccgc ataccaggga gtccccgacc
     1561 tcgagggttc ccttctgcac gaggacggtg gctacgggcc cgcgaccctt gtcgaggtgc
     1621 gcttccagga cggtcccccg cgcgcgaccg gtgggatcac ccttgagctc cgcgaggtcg
     1681 gcgacgatca ggacggtctc gagcaagccc tcgaggttcg cgctcgtctt ggccgagacg
     1741 tcgacgaact cggtgtcgcc tccccactcc gagggcacga gaccccgctc gaccatctgg
     1801 gtccgcacgc ggttcggatc cgcctcctcc ttgtcgatct tgttgaccgc aaccaccacg
     1861 ggcacaccgg ccgccttcgc gtggtcgagc gcctcgacgg tctgtggcat cacgccgtcg
     1921 tcggcggcga cgacgaggac cgcgatgtcg gtgatctggg cgccgcgggc acgcatcgcg
     1981 gtgaacgctt cgtggccggg agtgtcgatg aacgtgatct cccggccgtt gacgtgcgcc
     2041 tggtacgcgc cgatgtgctg ggtgatccct ccgaactcgc cggagacgac gtcggtcgta
     2101 cggatcgcgt cgaggagctt cgtcttcccg tggtcgacgt gccccatcac ggtcaccacc
     2161 ggtgagcggg gcacgagctc ggactcgtcg acctcgtccc cgagctcctc agcctggagc
     2221 tcgtcctcca tcccgacgat ctcgacgtga taaccgagct cggaacccag ggtctggagg
     2281 gcttcgtcgg acagcgacgt cgtcgcggtg accatctccc ccgccatgaa caggatcttc
     2341 acgatgtccg ccggcgatcg gttcgtcttc tccgcgaaga tctgcggcgt cgaaccgcga
     2401 acgacctgca agatcggccc ggcggggggg gccgggggtg cggcgggagc cgctccatcg
     2461 ggtacggcgg ccaatggggc tggcgtcgcc ggcacatcgg cggccgcagc cgtcggctcc
     2521 ggggcgccct cgtgctcctc ggtccgagcc cgctctgcgt cggggacggc gacgaccttc
     2581 ggggtcgctt tcggcttcgg cttcgccttc ggacgaggct tcggcggcgg ctccatgacc
     2641 tggcgcggct tcgctggctt cgtgaccttg ccacccgagg cacggaggcg aggcacgagg
     2701 tcttcgggca cgctggagga ggcggacttc cccgccacac ccatcttctc gagcgtctcc
     2761 agcagttcct tgctcgtgac gccgagctcc ttcgcgagct cgtggatcct catgtcgctt
     2821 gtgacgcctc ctcgatctcg ttcctaagcc tagcgagttc cccttgggtc aaccccgagc
     2881 gcagcgcgct cgccaccgct ccgcgccgga ccgcggcgtc ggcgcacgcc gggtctcgat
     2941 ggacgtacgc cccgcgaccg gacgccgacc cgatcgggtc cacctgcgcc cccgtctccg
     3001 atcggacgat gcggagcaga tcagccttgg gggcgcgctt ccggcacccg acgcacgacc
     3061 gctccggctc gcgcgccatg gttcctcatt ccgcctccgc gggcgcggcc g
//
LOCUS       DASUVE010000190         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_95025, whole genome shotgun sequence.
ACCESSION   DASUVE010000190 DASUVE010000000
VERSION     DASUVE010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95025"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VF036_04125"
     CDS             <1..1164
                     /locus_tag="VF036_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX6264450.1"
                     /translation="SCTFLGLVGMEDPIRPEVPAAIARCIDAGIRVVMITGDHPNTAR
                     SVARAVGIPGDRVVEGAELPESDEDLVELVADERLGILARVEPEQKLRVARALQAGGQ
                     VVAMTGDGVNDAPALRQADIGVAMGITGTDVAREAADLVLLDDNFAHIVEAVEEGRAA
                     FDNTRNFLTYHLTDNVAELAPFVIWALSAGSVPLLLSVLQVLALDIGTDLLPALALGA
                     EAPAKDAMRRPPRGTDEHLLDRHVLLRAFGWLGPVEALVSLSVALATAWLLFGWRPGG
                     PLPSAGTELAMLSGAVFASIVLMQMANAFECRSPSASAFERHRPMNRLLIAAVIAEAA
                     ALGLFLFVPALATILHQEAPPLPGWAIVCLAPILFVAAEETRKWIRSRRPADR"
     gene            complement(1262..2218)
                     /locus_tag="VF036_04130"
                     /pseudo
     CDS             complement(1262..2218)
                     /locus_tag="VF036_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
BASE COUNT          338 a          828 c          758 g          294 t
ORIGIN      
        1 tcgtgcacgt tcctcggact cgtgggcatg gaggatccga tacgtccgga ggtccccgcc
       61 gccatcgcgc gatgcatcga cgcggggatc cgcgtcgtga tgatcacggg cgaccatccg
      121 aacaccgcgc gatcggtcgc cagggccgtc ggcatccccg gcgatcgggt cgtcgagggc
      181 gccgagcttc cggagtccga cgaggacctc gtcgagctcg tggcggacga acggctcggc
      241 atcctcgcgc gcgtcgaacc cgagcagaag ctgcgggtcg cacgggcctt gcaggccggc
      301 ggccaggtgg tggcgatgac cggggacggg gtcaacgacg cgcccgcgct ccgccaggct
      361 gacatcgggg tcgcgatggg catcaccggt acggacgtgg cgcgggaggc cgccgatctc
      421 gtgttgctgg acgacaactt cgcgcacatc gtcgaagcgg tcgaggaggg gcgagccgcg
      481 ttcgacaaca cgcgcaactt cctcacctat cacctcaccg acaacgtcgc tgagctggct
      541 cccttcgtga tctgggcgct ctccgccggc tccgtccccc tcctcctgtc ggtgctgcag
      601 gtcctcgcgc tggacatcgg taccgacctc ctgcccgcct tggccctcgg cgcggaggcg
      661 ccggcgaagg acgcgatgcg gcgccccccg cgcgggaccg acgagcacct gctcgatcgc
      721 cacgtcctgc tgcgtgcgtt cggatggctg ggcccggtcg aagccctggt ctcgctgagc
      781 gtggcgctgg cgacggcatg gctcctcttc ggatggaggc cgggcggtcc gctcccctcg
      841 gccggcaccg aactcgcgat gctgagcggg gcggtcttcg cctcgatcgt cttgatgcag
      901 atggcgaacg cgttcgagtg ccggagtccg tccgcgtccg cgttcgaacg acatcgaccg
      961 atgaaccgcc tgctcatcgc ggccgtcatc gcggaagccg cggcgctggg gctcttcctc
     1021 ttcgtgccgg ccctcgccac catcctgcac caggaggccc ctcccctgcc cggctgggcg
     1081 atcgtctgcc tggccccgat cctcttcgtc gctgcggagg agacgcggaa gtggatccgc
     1141 tcccgacgac ctgcggatcg ctgaacgccg gtgctgcggc ctccacgcca cgccggcacc
     1201 gttcgaccgc gtcatccagg tccgcgacac gttccgtgtg gcccgaccag acccccgccc
     1261 gttcgagcag gcgctcgacg ccgggatcga ggcccgcgac gacgacctcg acgcccgacg
     1321 ccgccagctc gcccagggtg gcgccgagcg cgagggcacc ggtcgcatcg atcgccgaca
     1381 cggacgtcgc gtccaggacg aacgcacctc ccgaccgagc accggcacgc cggaggcggt
     1441 ccatgacacg ggctgcgttc aggtacacga cgggtccgga cgggcgagcg acgatgacat
     1501 cggcccggac ctcctcctcg acggcggccc ggagcagcgg gccgatactc acccgtccac
     1561 gcacgaccac gaccatgagg gcgaggccga tccccagcac gacgccctgg agcaggccga
     1621 tggagaccgc cacgagcgcg gtcacgatgc cgaccaacca ctcgagccca tcgagccgac
     1681 gcagctggcg gaacccccgc acgtccacga gtcgggcgac cgcaccgatc acgatggccg
     1741 ccaacgccgt cagggggagc gatgcgagcg ggtcggtgaa gaacatgagg atcgacgcca
     1801 tcagcgccgc cgagatgatc gcgcccacct gggagcggac gcccaccatc tcgacgatcg
     1861 cggtcctcgc ggcactcgcg ctgatcgtga tcccacccga gaacccggag gcggcgttcg
     1921 ccacgcccag acctcgcaga tcggtgctgg cgtcgacctc gtacccaccg cgatccgcga
     1981 acgcacggga cgacacgatc gtgtcggcgg aggcgagggc ggcgatcgcg atcgccgccg
     2041 ggatgagcgc cacggcatcg ccgagcccga cgtcgggcac accgatcctc ggcatcccgg
     2101 cggtgatcgg acccaccacc tcgacgtccg acaggccgtc gacgacggcg accgcgacgg
     2161 tcgcgacgag gatcaccgcg acggcggcga tcgtggcgtg ccggcgcctg gccgacac
//
LOCUS       DASUVE010000191         2770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_284392, whole genome shotgun sequence.
ACCESSION   DASUVE010000191 DASUVE010000000
VERSION     DASUVE010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284392"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1959)
                     /gene="ligD"
                     /locus_tag="VF036_04135"
     CDS             complement(<1..1959)
                     /gene="ligD"
                     /locus_tag="VF036_04135"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX6264451.1"
                     /translation="MPPRRRITSTGPAFTLDLPRQLDARKDGDAWWVEVDGRDLRLSN
                     LNKVFWPDEGYTKGDLLAYYWSIADLIGPHLEGRPLTMKRMPDGAEGPHFYEKTAPSH
                     TPDWVHRCQVHSDDSKRGRIDYLMVDDTATLLYVANLGCIEMHPLHSRCEDVAHPDYL
                     FFDLDPFEPYTYEDVLAVARHIKVVLDQLGLTAYPKTSGATGLQIYLPVKRGTYTYGQ
                     VRAFVGACGRMITKVDPDHVTMAWKIADRTGKVFIDHNMNRQGANIAAAYSLRPEPRA
                     PVSTPLAWDEVFEGGFEPQDFRIDNVWDRFAERGDLFEGVRTEAMDLSTAFDALDVRA
                     EDDGPGFSSALPRTAAPSVVRKTSEEIVEESRDANLYEYVRRRDFGEAGTTEPAPGEV
                     AGTGNSFVIHKHRATRLHYDVRLERDGGLPSWAVPRGLPTSKGDKRLAVETEVHPLEY
                     GAFEGTIPEGHYGAGEVRIFDDGWYEPVEWTDSKVSFRLHGRRYPGLEFHFVKTRTDW
                     LAFLASAQDAPRIATPPRFQPMLAEGGGEPFDDPRWRFEPKLDGIRALAEMETGGTVF
                     RTRRGRDVTFQYPDIHMIHELVDQVNAVLDGEIVAVEDGGRTSFELLQQRMNLQNERE
                     IKRVAKQIPVSFVAFDLLWLDGHDVTGLA"
     gene            2054..>2770
                     /locus_tag="VF036_04140"
     CDS             2054..>2770
                     /locus_tag="VF036_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX6264452.1"
                     /translation="MGDTATTNRQDEERRWRPRPLLSFALRAVSVLVPAAVGAASAYA
                     FVSALPIPRGLAVVPWALGLVATSTVATAIAELVGKRFLPLAVLFRLSLVFPDRAPSR
                     FAMARGAGNIRQLERRIRDARERGVDPEPVRAASEILALVAALSAHDRKTRGHSERVR
                     AFTDLLAGELKLPEADRDRLRWAALLHDIGKIHVPARILNKAGRPDAREWERLQAHPA
                     MGARIAEPLMTWLGPWGRAIE"
BASE COUNT          412 a          939 c          958 g          461 t
ORIGIN      
        1 ggccaggccg gtgacgtcgt gtccgtccaa ccagagcagg tcgaaggcga cgaaggagac
       61 cgggatctgc ttcgccaccc gcttgatctc ccgttcgttc tggaggttca tccgctgctg
      121 gaggagttcg aaggacgtcc ggcctccgtc ctccaccgcc acgatctctc cgtcgagcac
      181 ggcgttcacc tgatccacga gctcgtggat catgtggatg tcggggtact ggaacgtgac
      241 gtcacgaccc cggcgggttc ggaacaccgt gccgcccgtc tccatctcgg cgagggcgcg
      301 gatgccgtcg agcttcggct cgaagcgcca tcgcggatcg tcgaatggct cgcctcctcc
      361 ctcggcgagc atcggctgga accgtggcgg tgtcgcgatc cgtggagcgt cctgggcgga
      421 tgcaaggaac gcgagccaat ccgtccgcgt cttgacgaag tggaactcca accccggata
      481 gcgacggccg tggagacgga agctcacctt cgaatcggtc cactcgacgg gttcgtacca
      541 cccgtcgtcg aagatccgaa cctcgccggc gccgtagtgc ccctcgggga tcgtcccttc
      601 gaacgccccg tactcgagcg ggtgcacctc ggtctcgacg gcgagtcgct tgtcgccctt
      661 ggaggtcggc aggccgcggg ggaccgccca cgaggggagg ccgccgtcgc gttcgagccg
      721 gacgtcgtag tggagccggg tcgcgcgatg cttgtggatc acgaaggagt tgccggtccc
      781 ggccacctcg ccgggtgcgg gctcggtggt gccggcctcg ccgaagtcgc gccgccgcac
      841 gtactcgtac aggttcgcgt ccctcgactc ctccacgatc tcctcggagg tcttccggac
      901 gacggaagga gccgccgtcc tcgggagggc cgacgagaac ccgggaccgt cgtcctcggc
      961 tcgcacgtcg agcgcgtcga atgccgtcga cagatccatc gcctccgtgc ggacgccctc
     1021 gaagagatct ccgcgctcgg cgaaccggtc ccagacgttg tcgatccgga agtcctgggg
     1081 ctcgaagccc ccctcgaaca cctcgtccca cgcgagcggg gtcgagacgg gtgcgcgggg
     1141 ctcggggcgc aacgagtacg cggccgcgat gttggcgccc tgtcggttca tgttgtggtc
     1201 gatgaagacc ttccccgtgc ggtccgcgat cttccacgcc atcgtgacgt ggtcggggtc
     1261 gaccttcgtg atcatccgac cgcaggcgcc cacgaacgcc cgcacctgcc cgtaggtgta
     1321 ggtccctcgc ttcacgggca ggtagatctg gagcccggtg gctcccgagg tcttcggata
     1381 cgcggttagc cccagctgat cgaggacgac cttgatgtga cgagcgacgg cgaggacgtc
     1441 ttcgtaggtg tacggctcga acgggtcgag gtcgaagaac agatagtcgg gatgcgcgac
     1501 gtcctcgcag cgggagtgca acggatgcat ctcgatgcag ccgaggttcg cgacgtagag
     1561 cagcgtcgcg gtgtcgtcga ccatcaggta gtcgatccgg ccgcgcttcg agtcgtccga
     1621 gtggacctga cagcgatgca cccagtccgg tgtgtgcgag ggagccgtct tctcgtagaa
     1681 gtgtggcccc tccgcgccgt cgggcatccg cttcatcgtc aggggccgtc cttcgaggtg
     1741 cggtccgatc aggtccgcga tgctccagta gtaggcgagg aggtcgccct tcgtgtaccc
     1801 ctcgtccggc cagaagacct tgttcaggtt cgagaggcgg aggtcccgtc cgtcgacctc
     1861 gacccaccac gcgtcgccgt ccttgcgtgc atccagttgc ctgggcaggt ccagtgtgaa
     1921 ggcaggaccg gtcgacgtaa tccggcggcg tggcggcatc gccttcgatg ctacgtcgga
     1981 gggcgatatc ccccatccgg ggggtaaacc gtgtcggtgc ctcgaccgat acctcaatcc
     2041 tcggaggatc gcgatgggcg acacggccac gacgaaccga caggacgagg aacggcgctg
     2101 gcggccccgg ccactgctca gcttcgcgct gcgagccgtc tccgtgctcg ttcccgccgc
     2161 ggtcggggcc gccagcgcgt acgcgttcgt gagcgccctg ccgattccgc gggggctcgc
     2221 ggtggtcccg tgggccctcg gactcgtcgc gacgtcgacg gtcgccacgg cgatcgcgga
     2281 gctggtgggc aagcgcttcc tgccgctcgc ggtgctcttc cgactctccc tcgtcttccc
     2341 cgaccgcgcg ccgtcgcgct tcgcgatggc acgcggcgcg ggcaacatcc gccagctcga
     2401 acgccgtatc cgtgatgcgc gcgagcgcgg cgtggatccg gagcccgtca gagcggcgag
     2461 cgagatcctc gcgctcgtgg ccgcgctctc ggcacacgat cggaagacca gagggcacag
     2521 cgagcgcgtc cgagcgttca ccgacctcct cgcgggagag ctcaagcttc cggaggcgga
     2581 tcgggaccga ctccgatggg cggcgctcct ccacgacatc ggcaagatcc acgtgccggc
     2641 ccggatcctg aacaaggcgg gccgcccgga cgcgagggag tgggagcgcc tccaggcgca
     2701 tcccgcgatg ggcgcgcgca tcgccgagcc tctcatgacg tggctgggac cgtggggccg
     2761 agcgatcgag
//
LOCUS       DASUVE010000192         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1414898, whole genome shotgun sequence.
ACCESSION   DASUVE010000192 DASUVE010000000
VERSION     DASUVE010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414898"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VF036_04145"
     CDS             complement(<1..1071)
                     /locus_tag="VF036_04145"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX6264453.1"
                     /translation="MQRLGVLAKTYAIDMVIVALVVVGMLDTVLRWNTADPNPSGTFW
                     FALPAMAVLGIVILAHRRFPFAGPAAYWVLAAGISFVDPLLLPSAESFFAVGLVTAFL
                     LGSLRETRKAGIGLAIIVASTAVLVAMIPGHTTAEVFFIPIDFAVCWFAGLMLRGTAD
                     RAQAAEARAIVAERDREADALRAVVEERTRIARELHDIVGHSVSVMTVQTSGVRRLLH
                     PEQEQEREALLAVERTGREALAEMRRVVGTLRDPGGGPTLAPQPSLSRVDALVAHARE
                     TGLPVDLEIEGEPVSLPAGLDLTAYRLVQEGLTNAINHAAAAHAEVRVRYDDGHVEIE
                     VSDDGRGKSSGAPSNGGGHGLVG"
     gene            1263..1946
                     /locus_tag="VF036_04150"
     CDS             1263..1946
                     /locus_tag="VF036_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264454.1"
                     /translation="MNDRLGTWARRAVWALPVYGVLLAVGTITQQPDYRTDFAAYADY
                     ITTSTFLATHVIASIAGAAVGLIGVTALCALTEHVSPRRARWGFVSSAFGQIGLASLF
                     GVAAFAQPAIGRAFLDGERAVAETINADVYGSPLFATAALSLLAFVAGGIFLGGAARR
                     VGTWPRSAGVTFTTSITVFAVGVFVEVPFLQPVAAIGLTVASVAIARAAGVPDREGTV
                     AFADEVTVR"
     gene            1943..>2269
                     /locus_tag="VF036_04155"
     CDS             1943..>2269
                     /locus_tag="VF036_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264455.1"
                     /translation="MTAAGSVEKGVMIMKNARVFTAARIVALVVIGVLVVGLVSVGLG
                     GEESAVSVPAGAKAGDLLLHDCTYPTEVGDLPADCGTLVVAENASDPGSRLIALPIVR
                     VRARSDD"
BASE COUNT          315 a          780 c          811 g          363 t
ORIGIN      
        1 gccgaccagt ccgtgaccgc cgccgttcga tggcgcgccg ctgctctttc cccgcccgtc
       61 gtccgacacc tcgatctcga cgtggccgtc gtcgtagcgg acacgcacct cggcgtgcgc
      121 cgcggccgcg tgattgatcg cgttcgtcaa tccttcttgc acgaggcggt aggcggtcag
      181 gtcgaggccg gctgggagcg acaccggttc tccctcgatc tcgaggtcca ctgggaggcc
      241 ggtctcacga gcgtgggcga cgagggcgtc gactcggctc aggctcggtt gcggcgccag
      301 cgtcggcccg ccgcccggat cccggagcgt tcccaccacc cgtcgcatct ccgcgagcgc
      361 ctcgcgtccg gtccgttcga ccgcgagcag cgcctcgcgc tcctgctcct gctcgggatg
      421 gagcagccga cgcacgccgg acgtctggac cgtcatcacg ctgacgctgt ggccgacgat
      481 gtcatgcagc tcgcgtgcga tccgcgtgcg ttcctcgacg acggcacgca gcgcgtcggc
      541 ctcccggtcg cgctcggcga cgatggcccg ggcctcggcg gcctgggccc ggtcggccgt
      601 gccgcggagc atgaggcccg cgaaccaaca gaccgcgaaa tcgatcggga tgaagaagac
      661 ctcggccgtc gtgtgtccgg ggatcatggc aaccagaacc gcagtgctcg ccacgatgat
      721 cgcgaggccg atgccggcct tgcgagtctc gcggaggctg ccgaggagga acgccgtcac
      781 cagacccacg gcgaagaagc tctcggcgga cgggagcagc aacggatcga cgaacgagat
      841 gccggccgcg aggacccagt aggcggcggg acccgcgaac gggaaccgcc gatgcgccag
      901 gatcacgatc cctaggaccg ccatcgccgg caacgcgaac cagaacgtgc cggacgggtt
      961 cgggtccgcc gtgttccatc tgaggacggt gtcgagcatc ccgacgacga ccagggccac
     1021 gatcaccatg tcgatcgcgt acgtcttcgc gagcaccccg agccgctgca tgcgctgacc
     1081 gtactccgat gcgcttcgcc gcgcgtcctc ctcgaggacg accgtcgcgt cgaccttctg
     1141 gacgaccccc gtctccgcct tccgacgagc ggaatcggtc ctggggccga cgactgcgtg
     1201 gacctcgctc cctaccgtcc gacccggcgg gatcggtggg atcccggagg gaggtggatg
     1261 cgatgaacga tcgactcggg acctgggcac ggagggcggt ctgggcgctc ccggtgtacg
     1321 gcgtgctgct ggcggtgggc acgatcaccc agcaacccga ctatcggacc gacttcgccg
     1381 cgtacgcgga ctacatcacg acgtcgacgt tcctcgcgac ccacgtgatc gcgagcatcg
     1441 ccggcgcggc tgtcggcctc atcggggtga ccgcgctgtg cgcgttgacg gagcacgtat
     1501 cgccgaggcg cgcgaggtgg gggttcgtct cctcggcgtt cggacagatc gggctggcgt
     1561 cgctcttcgg cgtggcggcg ttcgcccagc cggcgatcgg tcgagccttc ctcgacggcg
     1621 agcgcgccgt cgcggagacg atcaacgccg acgtctatgg cagccctctg ttcgcgaccg
     1681 cggccctgag cctgctggcg ttcgtcgctg gcggcatctt cctgggaggg gcggcgcgtc
     1741 gcgtggggac gtggccccgc tcggcggggg tgacgttcac cacctcgatc accgtgttcg
     1801 cggtcggtgt cttcgtggag gtgccgttcc tccaaccggt ggcggcgatc ggactcacgg
     1861 tggcgagcgt cgcgatcgcc cgggcggccg gcgtcccgga tcgcgaagga acggtggcgt
     1921 tcgcggacga ggtgacggtc cgatgaccgc cgccggatcc gtcgagaagg gagtcatgat
     1981 catgaagaac gctcgagtct tcaccgcggc gcgcatcgtc gcgctggtgg tcatcggtgt
     2041 gctcgttgtc ggtctcgtct ctgtgggtct cgggggcgag gagtccgcgg tctcggttcc
     2101 cgccggagcg aaggccggag acctgctcct gcacgactgc acgtacccga cggaggttgg
     2161 ggacctgccg gccgactgcg gcacgttggt cgtggcggag aacgcttcgg atccggggtc
     2221 acgactgatc gcgttgccga tcgtccgcgt ccgagcccgg tcggacgat
//
LOCUS       DASUVE010000193         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_661409, whole genome shotgun sequence.
ACCESSION   DASUVE010000193 DASUVE010000000
VERSION     DASUVE010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661409"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1717
                     /locus_tag="VF036_04160"
     CDS             <1..1717
                     /locus_tag="VF036_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEX6264456.1"
                     /translation="FRVEGWIDTWATWRSDLEKRRDAGQDVAVELKEGATLVSGAVRR
                     ARAAGADADADRLREAASVMRRKDTAVGTTLALSDDFHALMSSHPDRRRGAAASQELE
                     MVAARERARHGAWYELFPRSASPDRGRHGTLRDVAALVPDIAELGFDVLYLPPIHPIG
                     RTSRKGPNNTAAAGPDDPGSPWAIGSEAGGHTAIHPELGTFDDLEHLLAELKRHEMEL
                     ALDLAFQCSPDHPWVTQHPEWFRHRADGTIRFAENPPKTYEDIYPIDFETKDREGLWR
                     ALLDVVLFWVDRDVSIFRVDNPHTKPFGFWEWLLAEVKRDHPDVLFLSEAFTRPRVME
                     HLAKIGFDQSYTYFAWRRTKDELTRYLIELTQAPVRDFLRPNLWPNTPDILTDQLQEG
                     GPPAFRLRFLLAATLGANYGIYGPAFEHMEGEPREPGSEEYLHSEKYEIRPRTPAAGA
                     LRELIARVNLIRREHPALQHDRTLRFHPVDGDQLLAYSKTLPDGKDPVVVIVNLDPVH
                     PRSGWVDLRMPELEMAWDASFTARDLLGGETFSWTGARNFVELHPGHQPGHVLAIEPD
                     EGRAP"
     gene            1852..>2153
                     /locus_tag="VF036_04165"
     CDS             1852..>2153
                     /locus_tag="VF036_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011840806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6264457.1"
                     /translation="MIYELHVRAYQDSNADGVGDLAGLVQRLDHIRDLGATAVWLLPF
                     YPSPLKDDGYDISAYKAIHPAYGTMRDFRTFLKEAHRRDLRVITEVVMNHTSDEHP"
BASE COUNT          346 a          778 c          717 g          312 t
ORIGIN      
        1 cttccgcgtc gaggggtgga tcgacacctg ggcgacgtgg cggtcggacc tcgagaagcg
       61 ccgagacgcc ggccaggacg tcgccgtcga gctcaaggag ggagcgacgc tcgtgagcgg
      121 ggcagtgcgg cgcgcccgcg ctgccggagc cgacgccgat gcggatcgac tcagggaggc
      181 cgcgtcggtc atgcgccgca aggacacggc cgtgggaacg acgctcgccc tgtccgacga
      241 cttccatgcg ttgatgtcct cgcatcccga tcgacgccgc ggggcggccg cctcgcagga
      301 gctcgagatg gtcgccgccc gcgaacgggc acgccacggc gcctggtacg agctcttccc
      361 gcgctcggcg tctcccgatc gcggccggca cggcacgctc cgcgacgtcg ccgcgctcgt
      421 gccggacatc gccgagctcg ggttcgacgt gctgtacctg ccgcccatcc atccgatcgg
      481 gcgcaccagc cggaaaggtc cgaacaacac cgcggcggcc ggacccgacg atccggggag
      541 tccgtgggcg atcggatcgg aagcgggtgg gcacaccgcg atccatccgg agctcggcac
      601 gttcgacgat ctcgagcacc tgctcgcgga gctgaagcgc cacgagatgg agctcgccct
      661 cgacctcgcg ttccagtgct cgcccgacca cccgtgggtc acgcagcatc ccgaatggtt
      721 ccgccaccgg gccgacggca cgatccgctt cgcggagaac ccgccgaaga cgtacgagga
      781 catctacccg atcgacttcg agacgaagga tcgggagggg ttgtggcgcg cgctcctgga
      841 tgtcgtgctg ttctgggtcg atcgcgacgt ctcgatcttc cgcgtggata atccgcacac
      901 gaagcccttc gggttctggg agtggctgct cgccgaggtg aaacgggatc accccgacgt
      961 cctgttccta tcggaggcgt tcacgcgtcc gcgggtgatg gagcatctgg ccaagatcgg
     1021 cttcgatcag tcgtacacgt acttcgcgtg gcgccggacg aaggacgagc tgacccggta
     1081 cctgatcgag ctcacgcagg cacccgtccg cgacttcctg cggccgaacc tgtggccgaa
     1141 cacgccggac atcctcacgg atcagttgca ggagggtgga cccccggcgt tccggctgcg
     1201 attcctcctg gcggcgacgc tcggcgcgaa ctacgggatc tacggacccg cgttcgagca
     1261 catggagggc gagcccaggg agcccggctc cgaggagtac ctccactccg agaagtacga
     1321 gatccgtccc cgcacccccg ccgccggtgc cttgcgcgag ctgatcgccc gcgtcaacct
     1381 gatccgccgc gagcatccgg ccctgcagca cgaccgcacg ctccggttcc acccggtgga
     1441 cggcgatcag ctgctcgcct acagcaagac gctccccgac gggaaggacc ccgtggtcgt
     1501 catcgtgaac ctcgatcccg tgcacccgcg atccggctgg gtcgacctgc ggatgccgga
     1561 gctcgagatg gcgtgggacg cgtccttcac cgcgcgtgac ctcctcgggg gggaaacgtt
     1621 ctcgtggacc ggtgcacgga acttcgtcga gctccatccc gggcatcagc cggggcacgt
     1681 cctcgcgatc gaacccgacg agggccgggc accgtgacgc ctcgcgtgcc aacgaccgcg
     1741 gagccgtcgg cctccgagac ccccgcgtcc accacggcga ccgatcccac gaccgaaccc
     1801 gccacggggc tcgcgtccct cggcgcgcct tctcgctggt accaggacgc cgtgatctac
     1861 gagctccacg tgcgcgccta ccaggacagt aacgccgacg gtgtgggcga cctggcgggg
     1921 ctcgtgcaac gcctcgacca catccgggac ctgggcgcga ccgccgtgtg gctcctgccc
     1981 ttctacccgt cgccgctcaa ggacgacggc tacgacatct ccgcgtacaa ggcgatccac
     2041 cccgcctacg gcacgatgcg cgacttccgg acgttcctga aggaggcgca ccgacgcgac
     2101 ctgcgggtga tcacggaggt cgtgatgaac cacacgtcgg acgagcaccc gtg
//
LOCUS       DASUVE010000194         6453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_848198, whole genome shotgun sequence.
ACCESSION   DASUVE010000194 DASUVE010000000
VERSION     DASUVE010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848198"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VF036_04170"
     CDS             complement(<1..195)
                     /locus_tag="VF036_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX6264458.1"
                     /translation="MLKLYTGLQTWWADLKASVTEEHGATAVEYALMVALIAVVIILA
                     VTFIGTSASTKFSEVGSAVNG"
     gene            515..1843
                     /locus_tag="VF036_04175"
     CDS             515..1843
                     /locus_tag="VF036_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264459.1"
                     /translation="MRAIDLAYHVKSGALALQTGDVIRTDPFTFTHGGLSWLNQQWGA
                     QVIFAAAHRFLGWMGVAMTYAAAIGAGFGFLYSSCRRAGARSRTAALLTLSGLLVATG
                     PPAPRPQALAVPLFTGTWLLLARRGRWTWLVPVLAMIWANVHGSFVLAPVLIIFALAD
                     DLIERRPANRTVLVLLATVAATFVNPFGLSVWSYAVEISSNETIRNWVAEWRPPDPLS
                     LSGGPFWLSGVVVVAIAVWKRAEVRPLDVAVLAFFFALGVPAIRGTLWWALAAPPVVS
                     RWFRSTEPVIDGPEERRPDTVRAIAAVCILALLPIAAILRSGVDPTTGTSQRLALDAP
                     EVLVDATRGALPVGSRLFVYQPFASWFEYSLSAYPVMVDSRIELFSNDVWHDYDRAIA
                     AAKGWEEILDRHRIEGVVLPPDAVLRDALSMATGWRRVVDGPAGSVFVRA"
     gene            complement(1844..2488)
                     /locus_tag="VF036_04180"
     CDS             complement(1844..2488)
                     /locus_tag="VF036_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6264460.1"
                     /translation="MVEPFAVVRAGLIATIDASPGLKVVASAASAEEAMGVIERVGRP
                     GLVILVSMGLAGEQDAFWLIRTLRERFPGHVILGMGANADPNAVSRTLFTGADGFVDK
                     NVEVEQFLSAIRHAGEEDVVIAGPAADSVGTIAEGIERRRSLAFRLTRREREVLVVAA
                     EGLTAREIADRLGVRERTVTTHLARIYGKLGVGNRLAALRIAARSGLVSLRQDE"
     gene            complement(2548..3201)
                     /locus_tag="VF036_04185"
     CDS             complement(2548..3201)
                     /locus_tag="VF036_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6264461.1"
                     /translation="MGDPVRAVVVAAHRTVRNLIEVACQEAGVTVVARAETAASAAEA
                     CRSSMPELLVLDLELPDADGFRVLDDLADVRPRSVLVLANNADGDRVLQALRVGARGY
                     VMKADGLRDLASTIRRVVAGERVMTPELEKDAIGALGRLARRARESADLEADLTQRQR
                     QVLELLGDGRTMGQIASRLGISPRTVETHVTSLYRKLGVRTRVQAISRAATLGLVEL"
     gene            complement(3218..3880)
                     /locus_tag="VF036_04190"
     CDS             complement(3218..3880)
                     /locus_tag="VF036_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6264462.1"
                     /translation="MDTIRILVVAESIGLTQDLVIAFRRRPGFMILGPVSDETAALAL
                     FGEVQPDIVLVQLDRLDGRGVAIISEIRSRTPVRVMAATRDPAAPEMELALAAGACGV
                     LPTDRTPPTLVSAFRRAVAGELVLPVDDLPPLVGLLQEARNRRNRQALLTTLTERERE
                     VMAAMAQGATTAGIALELGISPATVNTHVKNILGKLGVHSKVEAVGVAWRGGLGLGAR
                     TA"
     gene            3952..5961
                     /locus_tag="VF036_04195"
     CDS             3952..5961
                     /locus_tag="VF036_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX6264463.1"
                     /translation="MNRLAGETSPYLLQHAENPVDWFPWGDEALAVAESEERPIFLSI
                     GYSACHWCHVMERESFEDTGIAALLNANFVSIKVDREERPDLDSIYMDAVQAMTGQGG
                     WPLSAFLAPDGRPFFAGTYFPKEATHGLPSFRQVLEAIVAAWRDRREDVERQGNQVVE
                     AISRTLSVTASQEPLTDEISMNALAELRTDFDERWGGFGGAPKFPQPMTLEFVLRMAV
                     RGEPDALEMLTTTLDRIASGGIYDQVGGGFARYSTDAAWHIPHFEKMLYDNAQLAQLY
                     VRAWLFTREDRYRRVATDTLDYLLREMRHFEGGFFSSQDADSEGLEGKFFAWTWEELV
                     SLIGEDVARSFGATPAGNWEGTNVLWLPDGSEPSGLDLSAVRRTLFEARDARVRPGTD
                     DKVLTAWNAMAITALAEAGRAFEEPRFIDAAEGCGRFLLKNLRRADGRLLRSWREGIP
                     GRAGFCDDHALLASACLTLFSTTGGIHWFREAVVLLDALVDLFLDAERGGFFQTGSDD
                     DQLVLRPKELYDNAVPSGNSVAADVLQRVALLSGDPDREQVGVAAIQLVRDALGKAPT
                     AFGHALCALDLSLGPSREVAIIGDPDDIRTRALTDEVHRNRFLPNAVVAIGDPRHREA
                     MEAVALLRDRPQVEDAPTAYVCQRFACRLPVTRPEELAAQLEQKL"
     gene            complement(5990..>6453)
                     /locus_tag="VF036_04200"
     CDS             complement(5990..>6453)
                     /locus_tag="VF036_04200"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX6264464.1"
                     /translation="ARINDEAIQAAMSKIRDVVWNLEGKRVALLGLSFKPATDDVRFS
                     PSLILARSLLGEGTHVVGYDPVAGSAALVEVPELELAADPYEAATGAHCVVVCTAWDE
                     LRELDLRRLGSVMAAKNLVDGRNLLDAAAVNEAGFAYRAMGRPGSFGASGP"
BASE COUNT         1046 a         2200 c         2109 g         1098 t
ORIGIN      
        1 gccgttgacg gccgatccga cctcgctgaa cttcgtcgaa gcagacgtgc cgatgaaggt
       61 caccgccagg atgatcacca ccgcgatcag agcgaccatc aacgcgtact caaccgcggt
      121 cgcgccgtgc tcctccgtga cgctggcctt gaggtcggcc caccacgtct gaagacccgt
      181 gtagagctta agcatccgat acacctcctt cctccgagat cccgtcctcg gggctttcgc
      241 cccattggcg agctgctact ccgccagcga gaacgataga agagccccat cccgccgagg
      301 aatcccgtaa tccacgcggg ttcgctacgt ggttctacgt acgaggcggc tacgccggac
      361 ccgtgagggg agaccgtagg attcaccggt ggataccccc gcggtggctg agtctcgcga
      421 ccagcagaca caagccccgg cgcagcgaag ctcgcagcct ccctggtcgc tccttgcctg
      481 ggtacttccc gcttcggccg ccctcctgat caggatgaga gccatcgatc tcgcgtatca
      541 cgtgaagtcg ggggcgctgg ccctccagac gggcgacgtg atccggacgg atccgttcac
      601 gttcacgcac gggggtctct cgtggctgaa tcagcagtgg ggagcacagg tgatcttcgc
      661 cgcggcgcat cggttcctcg gctggatggg agtcgcgatg acctatgcgg ccgcgatcgg
      721 ggccggtttc ggcttcctct actcgagctg ccggcgggcc ggcgcacggt cacgaacggc
      781 agccctgctc acattgtccg ggctcctcgt cgccaccggc ccacccgccc cgcgaccgca
      841 agcactcgcc gtcccgctct tcacggggac gtggctcctc ttggcgaggc gtggcaggtg
      901 gacgtggttg gtcccggtct tggcgatgat ctgggcgaac gtgcatggaa gcttcgtgct
      961 cgcacccgtc ctgatcatct tcgctctcgc tgacgatctc atcgaacgaa gacccgccaa
     1021 ccggaccgtc ctcgtattgc tggcaaccgt cgcggcgacg ttcgtcaacc cattcggtct
     1081 cagcgtgtgg tcatacgccg tcgagatctc cagcaacgag acgatccgga actgggtggc
     1141 cgagtggcgt cccccggatc cactgtcgct gagtgggggt cccttctggc tgtccggcgt
     1201 cgtggtcgtc gccatcgcgg tgtggaagcg cgccgaggtc cgcccgcttg atgtagccgt
     1261 attggccttc ttcttcgctc tgggcgtgcc ggcgatccga gggaccctat ggtgggcgct
     1321 cgcggcgccc ccggtcgtct ctcgctggtt ccgctcgacg gaaccggtca tcgacggacc
     1381 cgaggagcgg cgaccagaca cggtcagagc catcgctgcg gtgtgtatcc tcgcgctcct
     1441 cccgatcgcg gcgatcctcc gctccggggt cgatccgacg accgggacct cgcagcgact
     1501 cgctctcgat gcccccgagg tcctggtgga tgcgacccgc ggggccctgc ccgtcgggtc
     1561 tcgcctcttc gtctaccaac cgttcgcctc ctggttcgag tactccctct cggcgtatcc
     1621 ggtgatggtg gattcgagga tcgagctctt ctcgaacgat gtgtggcacg actatgaccg
     1681 ggcgatcgca gcggcgaagg gctgggagga gatcctcgac cgtcaccgga tcgaaggggt
     1741 cgtcttgcca cctgatgccg tcttgcgaga tgcgctgtcg atggccaccg gctggaggcg
     1801 agtggtcgac ggcccagccg gctcggtgtt cgttcgagcc tgatcactcg tcctgacgca
     1861 acgacacgag tcccgagcgg gcagcgatcc ggagagcggc gagacgattc ccgactccga
     1921 gcttcccgta gatcctcgcg aggtgagtgg tcaccgtccg ctcgcgaaca cccaatcgat
     1981 ccgcgatctc ccgcgccgtc agtccctccg ctgcgaccac gagcacctca cgctcgcgtc
     2041 gcgtgaggcg gaaggccagg cttctccgcc gttcgatccc ctccgcgatc gtgccgacgg
     2101 agtcggccgc gggaccggcg atcaccacgt cctcctcccc agcgtggcgg atcgccgaaa
     2161 ggaattgctc cacctcgacg ttcttatcga cgaagccgtc ggcaccggtg aagagcgtcc
     2221 tggagaccgc gttcgggtcg gcgttcgccc ccatccccag gatcacatgc ccagggaatc
     2281 gctcccgcag ggttcgtatc aaccagaatg cgtcctgttc gccagcgaga cccatcgaca
     2341 cgaggatcac gagtccgggg cgcccgacac gttcgatgac ccccatcgcc tcctcggcgc
     2401 tcgccgcgga agccaccacc ttgagcccag ggctcgcatc gatcgttgcg atgagaccgg
     2461 cccgcacgac agcgaacggc tcgaccacca cgacatcgtg tggaggtctg tcttcgtcat
     2521 cacgcgctgg catcggccca ggtgaggcta caattcaacc aacccgagcg tggcggcgcg
     2581 agagatcgcc tgcacgcggg ttcgcacacc gagcttgcga tacaggctgg tcacgtgggt
     2641 ctcgacggtg cgtggagaga tcccgagcct ggaagcgatc tgccccatcg tcctgccgtc
     2701 ccccaagagc tcgagcactt gccgttgtcg ctgcgtgaga tccgcctcga gatccgcgct
     2761 ctccctggct cgccgagcga gacgcccgag cgcccctatc gcgtccttct ccagctcggg
     2821 ggtcatcacg cgttcgcccg cgaccacccg ccggatggtg gaagcgagat cgcggagccc
     2881 gtccgccttc atcacgtatc cgcgtgcgcc gacccggagc gcctgcagga cgcgatcccc
     2941 gtcggcgttg ttcgcgagca ccagcacgga ccgtggtcgg acatcggcga ggtcatcaag
     3001 gactcggaag ccgtctgcat cggggagctc gagatccaac acgaggagct ccggcatgct
     3061 cgagcggcat gcctcggccg ccgaagcagc tgtctctgcc ctggcgacga cggtcacacc
     3121 cgcctcctgg caagcgacct cgatcaggtt tcgaaccgtc cgatgagcgg cgaccacgac
     3181 cgctcgcacg ggatcgccca tggcaacagg ctagccctca agcggtgcgc gcgcctagtc
     3241 cgagaccccc gcgccatgcc acgccgaccg cctcgacctt cgagtgcact ccgagcttcc
     3301 caaggatgtt cttgacgtgg gtgttcaccg tcgcggggct gatcccgagc tcgagggcga
     3361 tcccggctgt cgtagcccct tgggccatcg ctgccatgac ctcgcgttcc ctctcggtga
     3421 gcgtcgtgag gagagcctgg cgattccgtc ggttgcgagc ctcttggagg aggcccacca
     3481 acggcggcag atcgtcaacc ggaaggacga gctccccggc gacggcgcgc cggaacgcgc
     3541 tcaccagggt gggcggcgtc cgatcggtgg ggaggacgcc acacgctcca gcagccagcg
     3601 cgagctccat ctccggcgct gccggatccc gagttgccgc catgacgcga accggtgtcc
     3661 tgctcctgat ctccgagatg atcgccactc cgcgcccgtc gagccggtcg agctggacga
     3721 gcacgatgtc gggttgcact tcgccgaaga gtgcgagcgc cgccgtctcg tcggacacgg
     3781 gaccgaggat catgaatccc gggcgcctgc gaaaggcgat caccagatcc tgtgtgagac
     3841 cgatgctctc ggcgacgacg aggatacgga tcgtgtccat ggcgtaccag gaacatcggc
     3901 atctcgggac ctcgagatga gccctcccgc gaaggttggg agactgacgt gatgaaccgt
     3961 ttggcgggag agaccagccc gtatctgctc caacacgcgg agaatcccgt ggattggttc
     4021 ccgtgggggg acgaggcgct cgccgtcgcg gaatcggagg agcgtccgat cttcctgtcg
     4081 atcggctact ccgcgtgcca ctggtgccac gtcatggagc gcgagtcgtt cgaggacacg
     4141 gggatcgctg ccctcctgaa cgcgaacttc gtctcgatca aggtggatcg ggaggaacga
     4201 cccgatctcg actcgatcta catggatgcc gtccaggcga tgaccgggca gggcggatgg
     4261 ccgctctcgg cattcctcgc gcctgacggt cgaccgttct tcgcgggcac ctacttcccc
     4321 aaggaagcca cccacggtct gccgtcgttc cgacaggtcc tcgaggcgat cgtcgcggca
     4381 tggcgggacc ggcgcgagga tgtcgaacgg cagggaaacc aggtcgtgga ggcgatctcg
     4441 aggaccttat cggtcaccgc ttctcaggag ccgctcaccg acgagatcag catgaacgcg
     4501 ttggccgagc tgcggaccga cttcgacgag cgatggggag gcttcggcgg tgcgccgaag
     4561 ttcccgcagc cgatgacgtt ggagttcgtc cttcggatgg ccgtacgcgg ggaaccggac
     4621 gcgctcgaga tgctgacgac gaccttggac cggatcgcct caggcgggat ctacgaccag
     4681 gtcggaggtg ggttcgcacg ctactccacg gacgcggcct ggcacattcc gcacttcgag
     4741 aagatgctct acgacaacgc gcagctcgcg cagctctacg tccgggcatg gttgttcacg
     4801 cgcgaggacc ggtaccgacg agtcgcgacc gacaccctcg actacctcct ccgcgagatg
     4861 cgtcacttcg aaggcggctt cttctccagt caggacgcag attccgaggg tctcgagggc
     4921 aagttcttcg catggacgtg ggaagagctc gtcagcctca tcggcgagga tgtcgcgcgc
     4981 agcttcggag ccacgccggc gggcaactgg gagggcacga acgtgctgtg gctgcccgac
     5041 ggttcggagc cgagtggcct cgacctctcc gcggtgcgac gcacgctctt cgaggcgcga
     5101 gatgcccggg tccgaccggg aacggacgac aaggtgctca ccgcgtggaa cgcgatggcg
     5161 atcacggccc tcgccgaggc cggccgagcg ttcgaggaac cccggttcat cgacgcggct
     5221 gagggctgcg ggcgcttcct cctgaagaac ctccggcggg ccgacgggag actcctgcga
     5281 tcctggaggg aggggatccc cggacgggcc ggcttctgtg atgaccatgc gctgctggcc
     5341 tccgcctgcc tgacgctgtt ctcgaccacg ggagggatcc actggttccg cgaagcggtc
     5401 gtgctcctgg acgcgttggt ggacctgttc ctcgacgcgg agcgcggtgg cttcttccag
     5461 acgggatcgg atgacgatca gctcgtcctc cgaccgaagg agctgtacga caacgccgtg
     5521 ccgtccggga actcggtggc cgccgacgtc ctccagcgag tcgcgctcct ctccggtgac
     5581 cccgaccggg agcaggtcgg cgtcgcggcg atccagttgg tccgcgacgc gttggggaag
     5641 gcgccgacgg cgttcgggca cgctctctgt gcgctcgacc tctctctcgg accctcgcgc
     5701 gaggtggcga tcatcgggga ccccgacgac atacgcacgc gtgcgctgac ggacgaagtc
     5761 catcgcaatc gcttccttcc caatgcggtc gtggcgatcg gggacccccg acaccgggag
     5821 gccatggaag cggtggcgct cctccgcgac cgcccccagg tcgaggacgc acccaccgcc
     5881 tacgtgtgtc aacggttcgc gtgtcgtttg cccgtcacgc ggcccgagga actcgcggcg
     5941 caactggagc agaagctgtg acggagcgaa gatcggtcca ctgagcgagc tacggtccgg
     6001 atgcgccgaa ggatcccgga cgtcccatcg ctcgataggc gaatccggcc tcgttcacgg
     6061 ccgccgcgtc cagcaggttc cgcccgtcaa cgagattctt cgctgccatc acgctgccga
     6121 ggcggcggag atcgagttcg cggagctcgt cccatgccgt gcagacgacg acacaatggg
     6181 cacccgtcgc cgcctcgtac ggatcggcgg ccagctccaa ctcggggacc tcgacgagtg
     6241 cagccgagcc cgcgacggga tcgtagccga cgacgtgggt tccctcaccg agcaatgacc
     6301 gtgccaggat gagcgacggg gagaaccgga catcgtccgt ggcgggcttg aaggagaggc
     6361 cgagcagcgc gacacgtttg ccctccagat tccatacgac gtcgcgtatc ttcgacatcg
     6421 cggcttggat ggcctcgtca ttgatgcggg cga
//
LOCUS       DASUVE010000195         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1038138, whole genome shotgun sequence.
ACCESSION   DASUVE010000195 DASUVE010000000
VERSION     DASUVE010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038138"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VF036_04205"
     CDS             <1..269
                     /locus_tag="VF036_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264465.1"
                     /translation="RLAVTEAASLLLEEGDATSLQMSIGRDGDTMEVTLTSDGRSDPW
                     PTDRVTASWPWLVMKGLTDAVRAERAEGAGPSIGFTKHRERVDR"
     gene            266..1012
                     /locus_tag="VF036_04210"
     CDS             266..1012
                     /locus_tag="VF036_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX6264466.1"
                     /translation="MSVRTGQTLSDEEIMELFRRLPEPSARDALAAEFLPLAEYFARR
                     FSGRGEPVDDLTQTASLGLLNAIDRYDPERGVPFSTYAAATIVGELKRHFRDRGWALR
                     VPRNIQETAIVVNRTVSSMWQDLGRAPTVAEIARAADIGEDDVLQALDALQAYTTDSL
                     DAPVGDASTTTAESIGSVDPSFQVSDDWLSIAPALRDLPERERKILYLRFFEGRTQTE
                     IAGELGISQMHVSRLVSQSLEKLRKRAEEV"
     gene            complement(1073..1567)
                     /locus_tag="VF036_04215"
     CDS             complement(1073..1567)
                     /locus_tag="VF036_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6264467.1"
                     /translation="MMLVRHAMAMSPVTASPDMNAGDAAGLMAANDIGVVPITDGDRL
                     VGIVTDRDLVLRVLAERKDPKAVRLGEIATTKGIETTSPDTQLCDARALMAERRIRRL
                     PVVKDDRLVGMLSLGDVAIGSASMRGVGETLASISTSRATEDTNAGPDIGTPARVKEA
                     REAS"
     gene            1682..2206
                     /locus_tag="VF036_04220"
     CDS             1682..2206
                     /locus_tag="VF036_04220"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEX6264468.1"
                     /translation="MTEEGKNQRAGSQADRRAAATTDPDESPKERVDRELIELFNGLR
                     VILPGVQVLLAFLLTVPFSSGFQDATSTDKIVYFTTVLLTVGATGALTMPAAYHRIRF
                     RQGDKEQMLRLSNTFAIVGLALLTLAMTTGVFLITNVLYGVIAAIAVSLVVFLMLGLS
                     WFAIPVFRRVENGG"
     gene            complement(2251..2589)
                     /locus_tag="VF036_04225"
     CDS             complement(2251..2589)
                     /locus_tag="VF036_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264469.1"
                     /translation="MSGSQADEERFEPRLKRRLSTGLVVGSLLGIAIGIAVGIFAFDG
                     RAGAVAACAIAGLIAGLLYGTIVGSFAGLESPDPGQEPSETSHPLSEPAVDEEREPDG
                     SGTRGADRPG"
BASE COUNT          409 a          928 c          905 g          434 t
ORIGIN      
        1 tccggctcgc ggtgaccgag gcggcgtcgc tcctgctcga ggagggcgac gcgacgtcgc
       61 tccagatgtc gatcggtcgc gacggcgaca cgatggaggt caccctcacg tccgacgggc
      121 ggtcggaccc gtggccgacc gatcgcgtca cggcgtcgtg gccgtggctc gtgatgaaag
      181 gactcaccga cgcggttcga gccgaacggg ccgagggcgc cggtccctcg atcggtttca
      241 ccaagcatcg tgagcgtgtg gatcggtgag cgtgcgtacg ggccagacat tatccgacga
      301 ggagatcatg gagctcttcc gtcgattgcc cgagccgtcc gcccgggatg cgctcgccgc
      361 ggagttcctc ccgctcgccg agtacttcgc tcgacgcttc tcgggcaggg gggagcccgt
      421 cgacgatctc acccagaccg cctctttggg cctgttgaac gcgatcgacc ggtacgatcc
      481 cgagcggggc gtgccgttct cgacctacgc ggcggccacg atcgtgggtg agctgaagcg
      541 gcacttccgc gatcgcggat gggcgctccg tgtccctcgg aacatccagg agaccgcgat
      601 cgtcgtcaat cgcaccgtgt cgtcgatgtg gcaggacctg ggtcgagctc cgaccgtcgc
      661 ggagatcgca cgggcggcgg acatcggcga agacgacgtc ttgcaagccc tcgatgcact
      721 gcaggcgtac acgacggact cgctcgatgc tccggtcggc gacgcgtcca ccaccaccgc
      781 cgagtcgatc gggagcgtgg acccgagctt ccaggtctct gatgactggc tgagcatcgc
      841 ccccgcgctc cgcgatctcc cggagcggga gcggaagatc ctctaccttc ggttcttcga
      901 ggggaggacc caaacggaga tcgccggaga gctcgggatc tcccagatgc acgtctcccg
      961 gctcgtctcc caatcgctgg agaagctccg gaagcgcgcc gaagaggtgt gacgcgggag
     1021 ccggacggag ggggcccgcg accgactccc gccccacgat cgagcggaac ggttacgacg
     1081 cctcccgcgc ctccttgacc cgcgcgggcg tgccgatgtc gggtccggcg ttcgtgtcct
     1141 cggtggccct cgacgtcgag atcgacgcga gcgtctctcc cacaccccgc atcgatgccg
     1201 atccgatcgc cacgtcaccc agggacagca tgccgacgag ccggtcgtcc ttgacgaccg
     1261 gtaaccgtct gatccgccgt tcggccatca gcgccctggc atcgcagagc tgcgtgtccg
     1321 gcgacgtggt ctcgatccct ttcgtcgtcg cgatctcccc caagcgcacg gccttcgggt
     1381 ctttccgctc cgcgagcacg cggagcacca gatcgcggtc cgtcacgatc ccgacgagcc
     1441 gatcgccgtc ggtgatgggg acgaccccga tgtcgttcgc ggccatgagg ccggcggcgt
     1501 cgcccgcgtt catgtcgggt gacgcggtca cgggagacat cgccatcgcg tgtctgacga
     1561 gcatcatggg gaacacgtcc tctccgttgc tcggttccgc cttcctccgt gccctcccag
     1621 cgttcccgcc gacgccatcg accaaacgcg gcaccttccg tgggtggggt accgtgaccc
     1681 ggtgaccgag gaggggaaga accaacgagc gggctcccaa gcggatcgtc gagcggcggc
     1741 caccacggac cccgacgagt cacccaagga gcgcgtcgat cgcgagctga tcgagctgtt
     1801 caacggcctc cgtgtcatcc tgcccggcgt gcaggttctg ctcgccttcc tgctcaccgt
     1861 tccgttctcg agcgggttcc aggacgcgac ctcgaccgac aagatcgtgt acttcaccac
     1921 ggtcctgttg acggtgggcg cgacgggggc gctcacgatg cccgctgcct accaccggat
     1981 ccgattccgg caaggggaca aggagcagat gctgcgtctg tcgaacacgt tcgcgatcgt
     2041 cggcctcgcg ctgctcacgc tcgcgatgac gaccggggtc ttcctcatca cgaacgtgct
     2101 ctacggtgtc atcgccgcga tcgcggtgag cctcgtcgtc ttcctgatgc tgggcttgtc
     2161 ctggttcgcg atcccggtgt tccggcgcgt cgagaacggg ggctgagacc gaccgaggtc
     2221 gcggcaccgg gatccagcga gtggggcccg tcagccaggc cgatccgcgc cgcgcgttcc
     2281 gctgccgtcc ggctcgcgct cctcgtccac ggcgggttcc gacagggggt gcgacgtctc
     2341 ggagggctcc tgccccggat ccggcgattc caagccggcg aacgaaccca cgatcgtgcc
     2401 gtagagcagt cctgcgatga ggccggcgat ggcgcaggcg gcgacggcac cggctcgccc
     2461 gtcgaacgcg aagatcccca cggcgatccc gatcgcgatc cccaggaggg agccgacgac
     2521 gagacctgtc gacagcctgc gcttcaggcg aggctcgaag cgttcctcgt cggcctgcga
     2581 tccgctcatg agaagggagt gtcgccgggc ccgcccgcac ggaggagcgg gcgggcccgg
     2641 acgacgcgag aagagtgaac ctcggggaag gtgtcg
//
LOCUS       DASUVE010000196         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_285536, whole genome shotgun sequence.
ACCESSION   DASUVE010000196 DASUVE010000000
VERSION     DASUVE010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285536"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..>2050)
                     /gene="acnA"
                     /locus_tag="VF036_04230"
     CDS             complement(81..>2050)
                     /gene="acnA"
                     /locus_tag="VF036_04230"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX6264470.1"
                     /translation="TATDLVLTLTQMLRAHGVVGRFVEFFGVGLSSLAIADRATLSNM
                     CPEYGATSAYFPVDDRTLEYLRFTGRGALIDRVERYTKAQGLFRTDADAPPTFTETLD
                     LDLSAVEPSMAGPRRPQDRVALPDVWDSFVDAFQRHLEPEPKPTELGRFVAEGGTPAA
                     DGEQRGWTEDVTPNGDDVRHGSVVIAAITSCTNTSNPSVMVGAGLLAKRAVEAGLASK
                     PWVKTSLAPGSRVVTDYLDRAGLTPYLEKLGFDLVGFGCTTCIGNSGPLPDDVAARID
                     EKNLNVVAVLSGNRNFEGRIHPQVRASYLASPPLVVAYALAGRIDFDPTTEPLGEGND
                     GPVFLRDLWPSPADVADVIAEAVSEDQFVQQYAKIWDGDERWAALPTPTGPAYDWDPD
                     STYVREPPFFLKASADPVGVGEVEGSRIEGARILVAVGDSITTDHISPAGAIRPDSPA
                     GRYLLEHEVPQRDFNSYGARRGNHEVMMRGTFANVRLRNALAPETEGPWTTHLPSGEV
                     TSVYEAAVRYAEEGTPLGVLAGKEYGSGSSRDWAAKGPALLGVRFVLAESFERIHRQN
                     LVGMGILPLQYKEGGSAAAHGLSGREIYSIQGLEIGIAPGQDATVEVVREDGSSASFP
                     VTVRIDQAAEVAIFGHGGILPMVLREALGAG"
BASE COUNT          305 a          742 c          697 g          306 t
ORIGIN      
        1 acgaccgatc ctgtgtacac ggcggagctg gccgcccagt aggtcgcctc acgggggttc
       61 gtcgcgcggt ctcgaggtcc ctagccggcc ccgagcgcct cgcggagaac catcggcagg
      121 atgccgccgt gaccgaagat cgccacctcc gccgcctggt cgatccggac cgtcaccggg
      181 aacgaagccg acgacccgtc ctcgcgcacg acctccaccg tagcgtcctg tccgggcgcg
      241 atcccgatct ccaggccctg gatcgagtag atctcccggc cggagagacc gtgggccgcg
      301 gcggatcccc cctccttgta ctggagcggg aggatgccca tgccgacgag gttctgtcga
      361 tggatccgct cgaaggactc ggccaggacg aaccggacgc cgaggagcgc cggtcccttc
      421 gccgcccagt cgcgtgacga cccgctcccg tactccttgc ccgcgaggac gcccagcggc
      481 gtcccctcct ccgcgtagcg gacggcggcc tcgtagacgc tggtcacctc gccgctcggg
      541 agatgggtcg tccacggccc ctcggtctcc ggcgcgagcg cgttccgcag acgcacgttc
      601 gcgaacgttc cccgcatcat cacctcgtgg ttcccgcgcc tcgccccgta cgagttgaag
      661 tcccgctgcg gaacctcgtg ctcgaggagg taccgaccgg ccggtgagtc gggccggatc
      721 gcgcccgcgg gcgagatgtg atcggtggtg atggagtcgc ccacggcgac gaggatccgg
      781 gcgccctcga tgcgcgaacc ctcgacctcc ccgaccccca ccggatccgc ggacgccttc
      841 aggaagaacg gcggctcgcg cacgtacgtc gagtcggggt cccagtcgta ggccggcccg
      901 gtcggcgtcg ggagcgcggc ccatcgctcg tcgccgtccc agatcttggc gtactgctgg
      961 acgaactggt cctccgagac cgcttcggcg atcacgtccg ccacgtctgc cgggctcggc
     1021 cagaggtccc ggaggaagac cggaccatcg ttcccctcgc ccaggggttc cgtcgtcgga
     1081 tcgaagtcga tcctgccggc gagggcgtag gccacgacca ggggcggcga cgccaggtaa
     1141 ctcgcgcgaa cctgcgggtg gatccgccct tcgaagttgc ggttcccgga caggaccgcg
     1201 acgacgttca ggttcttctc gtcgatccgc gccgcgacgt cgtccgggag cggcccggag
     1261 ttcccgatgc acgtcgtgca gccgaacccc acgagatcga agccgagctt ctcgaggtac
     1321 ggggtcagac ccgcgcggtc caggtagtcg gtcacgaccc gcgaccccgg tgcgagcgac
     1381 gtcttcaccc acggcttcga cgcgagaccg gcctcgaccg cgcgcttggc gagcaagccg
     1441 gcgccgacca tcaccgaggg gttggacgtg ttcgtgcacg acgtgatcgc ggcgatcacg
     1501 acggagccgt gtcggacgtc gtcgccgttc ggcgtcacgt cctcggtcca ccctcgttgc
     1561 tccccatccg cggccggggt ccctccctcg gcgacgaatc gaccgagctc ggtcggcttc
     1621 ggttcgggct cgagatggcg ctggaaggcg tcgacgaacg agtcccagac gtccgggagg
     1681 gccactcgat cctgcggcct gcgcggaccc gccatcgagg gctcgaccgc cgacagatcg
     1741 aggtccaacg tctccgtgaa ggtcggcggt gcatcggcat cggtccggaa cagcccctgc
     1801 gccttcgtgt atcgctcgac gcgatcgatc agcgctcccc gtcccgtgaa ccggaggtac
     1861 tcgagcgtcc ggtcgtcgac cgggaagtag gccgaggtgg ccccgtactc gggacacatg
     1921 ttcgacagcg tcgctcgatc ggcgatggcg agggaggaga gaccgacccc gaagaactcg
     1981 acgaaccgcc ccaccacgcc gtgggcgcgg agcatctggg tcagcgtcag gacgaggtcg
     2041 gtcgcggtcg
//
LOCUS       DASUVE010000197         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_285668, whole genome shotgun sequence.
ACCESSION   DASUVE010000197 DASUVE010000000
VERSION     DASUVE010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285668"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..98)
                     /locus_tag="VF036_04235"
     tRNA            complement(22..98)
                     /locus_tag="VF036_04235"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(62..64),aa:His,seq:gtg)
     gene            202..1581
                     /locus_tag="VF036_04240"
     CDS             202..1581
                     /locus_tag="VF036_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX6264471.1"
                     /translation="MAVKETIDFDLDLIDRLIADEEATLEPKHRASIEYRSVAERTVA
                     GGVASSWQASPPHAIFIDRGKGSHIWDLDGNEYVDYHLGYGAMVIGHAHPRVVEAIEK
                     RARLGTHFAQPTAQLHEIGENVAERFGLPLWRFCNSGTEATLEAVRLMRANTGRDLIV
                     KIEGTYHGHHDSLMFSVAPDPALIGPREHPATVPQAMGIPQTFADLVRVVPFNDLAEA
                     ERAFVENDGRVAGMIVEPAMMNCGVVLPQPGYLQGLLDLCHRHGAYLAFDEVKTGGTI
                     AWGGAVEAFGVRPDIVTLAKAFGGGVPCGAIGATKELFGPVLRGEHDIAGTFNGNPLT
                     MAAARATLLEVLTPDAYARLDSIDRALKDGIRPIIDRYRLPAYVTGIGAKGSVTYSAR
                     EVREYRDTIGIDERITYLAWLMQQNRGVFKSPWAKMETWTLSIVHSDEDARRYVENFE
                     AFAAAVSGA"
     gene            complement(1570..>2130)
                     /locus_tag="VF036_04245"
     CDS             complement(1570..>2130)
                     /locus_tag="VF036_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264472.1"
                     /translation="TTDTLSDPLPDPVDEVLDTTTDTVEGVSGSASGIVDPVSDATSG
                     TVDSVAGSTTDVASGVTDAVGTPDSGLSDPTLPAGASSAGSSVSDRTGSASSTRAMEF
                     GPSGGAALLEAREGADPAAEVGSSQGAQDSLTGAVADILRKLLAFTGWGALSWIAVAL
                     GLSIVGVAAVYHGRRRPRLSGSASRS"
BASE COUNT          348 a          724 c          745 g          313 t
ORIGIN      
        1 ggcccgcggc cacctcacgt ctggggtgac cgaggggact tgaaccccca gcctccagga
       61 ccacaacctg gcgctctaac cagttgagct acggccacca cgtgcgagcc ccaagcgtac
      121 cggaagcgac ctcccgggtt gccccgggac cgctggtcgg gcatcattga acggccgttc
      181 aattcccgga aggtgggtcc gatggccgtc aaggaaacga tcgacttcga cctcgatctg
      241 atcgatcgcc tcatcgccga cgaggaggcg accctcgagc cgaagcatcg cgcctcgatc
      301 gagtaccgct cggtcgccga acggaccgtg gccggaggcg tcgcatcctc ctggcaggcg
      361 tcccccccgc acgcgatctt catcgaccgg ggcaagggga gccacatctg ggatctcgac
      421 ggcaatgagt acgtcgacta ccacctcggg tacggcgcga tggtgatcgg gcacgcccac
      481 ccgagggtcg tggaggcgat cgagaagcgg gcgcgcctcg gcacccactt cgcgcagccc
      541 accgcccagc tccacgagat cggggagaac gtcgccgagc gcttcgggct ccccctgtgg
      601 cggttctgca actccgggac cgaggcgacg ctcgaggccg ttcgcctgat gcgggcgaac
      661 accggacgcg acctgatcgt gaagatcgag gggacgtatc acggccatca cgactcgctg
      721 atgttctcgg tggcgccgga cccggcgttg atcggtcctc gggagcatcc ggccacggtg
      781 ccgcaggcga tggggatccc gcagacgttc gcggatctcg tccgcgtggt gccgttcaac
      841 gacctcgcgg aagctgagcg cgcgttcgtc gagaacgacg ggcgcgtcgc cgggatgatc
      901 gtcgagcccg cgatgatgaa ctgcggagtc gtgctgccgc agccgggcta cctccagggc
      961 ctgctcgacc tctgtcaccg ccacggcgcc tacctcgcgt tcgacgaggt gaagaccggc
     1021 ggcacgatcg cgtggggagg cgccgtcgag gcgttcggcg tccgtcccga catcgtgacg
     1081 ctcgcgaagg cgttcggcgg aggcgtcccg tgcggcgcga tcggcgcgac gaaggagctc
     1141 ttcggtcccg tcctgcgcgg tgagcacgac atcgcgggca cgttcaacgg caacccgctg
     1201 acgatggccg ccgcgcgggc gacgctgctc gaggtcctca cgccggacgc gtacgcgcgg
     1261 ctggattcga tcgaccgcgc gctgaaggac gggatccgcc cgatcatcga ccgctaccgg
     1321 ctgcccgcgt acgtcacggg gatcggcgcg aagggatcgg tcacctactc ggcgcgcgag
     1381 gtgcgtgagt accgcgacac gatcgggatc gacgagcgga tcacctacct cgcgtggctg
     1441 atgcagcaga accgaggggt gttcaagtcg ccgtgggcca agatggagac gtggacgctc
     1501 tcgatcgtgc attcggacga ggacgcccgc cgctacgtgg agaacttcga ggcgttcgcg
     1561 gcggctgtgt caggagcgtg aggccgagcc gctcaatcgg ggtcgacgtc tcccgtggta
     1621 cacggcggcc actcccacga tcgagagtcc gagtgccacg gcgatccagg agagcgcgcc
     1681 ccagccggtg aaggccagaa gcttccggag gatgtcggcg accgctcccg tcaacgagtc
     1741 ctgggcaccc tggctggagc cgacctcggc ggccggatcc gccccttccc gtgcctccag
     1801 cagggccgct ccgccggagg gcccgaactc catcgccctg gtcgacgacg ccgagcctgt
     1861 ccgatcgctg acagaagacc cggctgagga cgctcccgcc ggcagggtcg gatcgctcaa
     1921 gcctgagtcg ggcgtcccga cggcgtccgt gactccgctg gcgacatccg tggtcgagcc
     1981 ggcgaccgaa tccacggttc cggatgtagc gtcggagacg gggtccacga tcccggaggc
     2041 ggagccagag acgccttcga ccgtgtcggt cgtcgtgtca aggacctcgt ccacggggtc
     2101 gggcagaggg tcagaaaggg tatcggtcgt
//
LOCUS       DASUVE010000198         4858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1038981, whole genome shotgun sequence.
ACCESSION   DASUVE010000198 DASUVE010000000
VERSION     DASUVE010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038981"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..578)
                     /locus_tag="VF036_04250"
     CDS             complement(12..578)
                     /locus_tag="VF036_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015795587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX6264473.1"
                     /translation="MAEATKEIGGLPLPPPGKYELDLAHTAVEFVARHILTKVRGRFT
                     DFSGWIDVAENPEESSAHVEIKTASIQTNTEQRDEHLKSDDFLNVEQWPVMTFTSTGV
                     RHTGGATFELDGELTIRDVTRPVTLAGEYLGTETSPYGKTVLAATARTTLKREDWNVN
                     WNMVLETGGFLVSKEIGLEIEVEALKVG"
     gene            716..1039
                     /locus_tag="VF036_04255"
     CDS             716..1039
                     /locus_tag="VF036_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6264474.1"
                     /translation="MDTERAVCESFQQAAQLVGKRLVPQIAWVLLGGPRRYSVLRAAI
                     PEISDTVLSERLRELEAAGILTRAVTPSTPVLIEYALTERGRELAKVIDGLAQWADRW
                     AVVGA"
     gene            complement(1052..2380)
                     /locus_tag="VF036_04260"
     CDS             complement(1052..2380)
                     /locus_tag="VF036_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6264475.1"
                     /translation="MSEVQPLSAAEIVEKSKRYTLYDWQAQSKASPIAVDRAEGVYFW
                     DVDDTRYLDFNSQLMGVNIGHGDKRVADAIARQAEKLPYISPFMAYETRARLGEKLAA
                     LWPGDLEKSFFTLGGAEANENAIRIAKAFTGRQKILARYRSYHGATYATINLTGDPRR
                     WANENPPMPGIVHVLDPYRGTARGTEDAATALATLEETIELEGPQTIAAFILETVTGT
                     NGILIPPDGYLEGVRDLCDRYGILFIADEIMCGFGRTGEWFAVNHYDLVPDLMTAAKG
                     LTSSYLPLGAVAIKPEIAAHFEENVFYGGLTYNSHPLSCAAAIAAIQVLEEDDLVGNA
                     KRLDPVMRRHHEELMAKHSSVGRVRNIGLFGIIELVKNRDTMEPLSPYTVVNETLQAV
                     NKGLLQRGLFTMIRFNGIMTNPPLCITEEQLDDGFAIIDEVLDIADAATG"
     gene            complement(2441..3670)
                     /locus_tag="VF036_04265"
     CDS             complement(2441..3670)
                     /locus_tag="VF036_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6264476.1"
                     /translation="MPSAEPLVVLAGHLLDVTTGEVLSDRALVLDRDRIRDVVEAGAA
                     PRGRTIDLSGRTVLPGMIDCHAHLIGELDDGQGYGYLVTRSSTQEALTGVTNARATLL
                     GGFTTVRDVGTFHAFVDLALRDAIEEGRLAGPRMMCAGAYVTCPGGGGDITGLPPDVL
                     VPSELRMGVSTGPDQVRANVRRIFEAGADFIKVIATGAVLTSGTNPGEPELTEEEIRA
                     AVEEAEARGTHVAAHAHGAEGAKRAIRAGVRSIEHGCLIDDETIDLMLERGTYLVCDM
                     FDGDWMLQEGPRLGYSDEVMRKTEMTNDAQRENFSKAVKAGVRIAFGTDSGIYPHGMN
                     ARNLAFHVRFGQTPMEAIRSATLDAAELLGWDDRVGSLRPGLFADLIAVDGDPLDDVT
                     VLEDVPFVMKGGQVVKG"
     gene            3764..>4858
                     /locus_tag="VF036_04270"
     CDS             3764..>4858
                     /locus_tag="VF036_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264477.1"
                     /translation="MVRRKHHASIVAGSALLILASLFGAVSLPAAAVEPSWFDLPASD
                     SFPQGLAAGPDGKTWVASRAAAEIARVAPDGSSSSVPLESGVDPYDIARGSDGAMWFT
                     EHNGNRIGRLTIDGELSEFYLRDLSTPTGITLGPDGAIWFAQRGVGAIGRITPDGEFT
                     EWRTITPRAAPLSVTTGSDGAIWFTLTAANRIGRIALDGTMTEFPLPANASGPQWITS
                     GPDGALWFTARASNAIVRMTIGGAVTTYPVPTPAAGVNGITVGADGAIWFTESTADAV
                     GRIGLDGAIVEIPLGEGATPTGITTGADGAIWFSAQGTNRVGRLEPSGPADRAAPTVT
                     IVSPARGSVLTDGEGMVADYFCTDEAGGSGV"
BASE COUNT          710 a         1690 c         1628 g          830 t
ORIGIN      
        1 ctcagccggc ctcagccgac cttgagcgcc tcgacctcga tctcgaggcc gatctccttg
       61 ctgacgagga agccgccggt ctcgaggacc atgttccagt tcacgttcca gtcctcgcgc
      121 ttcagcgtgg tccgagccgt cgctgcgagc acggtcttcc cgtacgggct cgtctccgtg
      181 cccaggtact cacccgcgag cgtcacggga cgggtcacgt cgcggatcgt cagttcaccg
      241 tccagctcga acgtggctcc gcccgtgtgt cgcacgccgg tgctggtgaa cgtcatcacg
      301 ggccactgct cgacgttcag gaagtcgtcg ctcttcaggt gctcgtcgcg ctgctccgtg
      361 ttcgtctgga tgctcgcggt cttgatctcg acatgggcgg aggactcctc ggggttctcc
      421 gcgacatcga tccagcccga gaagtccgtg aaccggcctc gcaccttggt gaggatgtgt
      481 cgggcgacga actccaccgc cgtgtgggcg aggtcgagct cgtacttgcc cggaggtggc
      541 aacgggaggc caccgatctc cttggtcgct tctgccatgg cagagactcc ttcctctcgg
      601 acgacgcagc ctcttggggc ccgtcggatg tcctcctcgg tcctgtaccc ggaaccgccg
      661 atcggtactc agtattcctc agtatctacg aagacctgag cgataggata gggcgatgga
      721 tacggaacga gccgtctgcg agagcttcca gcaggccgcc cagctcgtcg gcaagcgttt
      781 ggtgccccag atcgcgtggg tgctcctggg cgggcctcga cggtactccg tcctgcgggc
      841 cgcgatcccg gagatctcgg acacggtgct ctccgagcgc ctgcgggagc tggaagcggc
      901 ggggatcctc acccgtgcgg tgaccccatc cacaccggtg ctgatcgagt acgccctgac
      961 ggaacggggg cgcgagctcg cgaaggtgat cgacggcctg gcgcagtggg ccgaccgctg
     1021 ggcggtcgtc ggcgcctgac ttcgctgtcg ttcagccggt cgcagcgtcg gcgatgtcga
     1081 ggacctcgtc gatgatcgcg aagccgtcgt ccagttgctc ctccgtgatg cagagcggcg
     1141 ggttcgtcat gatcccgttg aagcggatca tcgtgaacag tcctcgctga aggagcccct
     1201 tgttcacggc ctgcaacgtc tcgttcacga cggtgtacgg cgagagcggc tccatcgtat
     1261 cccggttctt gacgagctcg atgatcccga agagaccgat gttgcggacg cgacccacgc
     1321 tggagtgttt ggccatcagc tcctcgtgat gcctgcgcat caccgggtcc agccgcttcg
     1381 cgttcccgac gaggtcgtcc tcctcgagga cctggatcgc cgcgatcgcc gccgcgcacg
     1441 agagcggatg ggagttgtac gtgagtcctc cgtagaagac gttctcctcg aagtgggcgg
     1501 cgatctcggg cttgatcgcg acggccccga gcggcaggta ggacgaggtc aggcccttgg
     1561 cggcggtcat caggtctggc acgaggtcgt agtggttcac ggcgaaccac tcgccggtcc
     1621 gtccgaaccc gcacatgatc tcgtccgcga tgaacaggat gccgtaccga tcgcagaggt
     1681 cccggacgcc ttcgaggtag ccgtcgggcg ggatcaggat cccgttcgtc cccgtgaccg
     1741 tctcgaggat gaacgccgcg atcgtctggg gtccctcgag ctcgatcgtc tcctccaatg
     1801 tcgcgagggc cgtcgccgca tcctcggtgc cgcgtgcggt gccccggtac ggatcgagga
     1861 cgtgcacgat cccgggcatc ggtgggttct cgttcgccca gcggcgtgga tcgccggtca
     1921 ggttgatcgt ggcgtaggtg gcgccgtggt acgagcgata cctcgccagg atcttctgcc
     1981 gtccggtgaa cgccttcgcg atccggatcg cgttctcgtt cgcctccgcg ccgccgagcg
     2041 tgaagaagga cttctcgagg tcgcccggcc agagcgcagc gagcttctct cccagccgtg
     2101 ctcgggtctc gtacgccatg aacggcgaga tgtaggggag tttctccgcc tgccgcgcga
     2161 tcgcgtcggc gacgcgcttg tccccgtgcc cgatgttcac gcccatcagc tgcgagttga
     2221 agtcgagata ccgcgtgtcg tcgacgtccc agaagtacac gccctcggct cgatccaccg
     2281 cgatcggcga agccttcgac tgggcttgcc agtcgtagag ggtgtagcgc ttcgacttct
     2341 cgacgatctc ggcggcggac agcggctgga cttcgctcat cggctcgacc ccctcgtgcg
     2401 gacgcgcgtc atcatcgcag acaccggctg ccgggtccgg tcagcccttg acgacctgac
     2461 cgcccttcat cacgaaggga acgtcctcga gcaccgtcac gtcatcgagg ggatcgccgt
     2521 cgaccgcgat caggtcggcg aacaggcccg gtcgcaagga ccccacgcgg tcgtcccaac
     2581 ccaggagctc ggccgcgtcg agggtcgcgc tccggatcgc ttccatcggc gtctgaccga
     2641 accgcacgtg gaacgcgagg ttcctcgcgt tcatcccgtg cggatagatc ccggagtccg
     2701 tcccgaaggc gatcctgaca cccgccttca cggccttgga gaagttctcg cgctgcgcgt
     2761 cgttcgtcat ctcggtcttc cgcatcacct cgtccgagta gccgaggcgt ggtccctcct
     2821 ggagcatcca gtcgccgtcg aacatgtcgc agacgaggta cgtccctcgc tcgagcatca
     2881 ggtcgatcgt ctcgtcgtcg atgagacagc cgtgctcgat cgagcggaca ccggcgcgga
     2941 tcgcccgctt cgcgccctcg gcaccgtgcg cgtgggcggc gacatgcgtc ccccgggcct
     3001 cggcctcctc caccgccgcg cggatctcct cctcggtcaa ttcgggctcg ccggggttcg
     3061 tcccggacgt gagcacggcg cccgtcgcga tcaccttgat gaagtcggcg cccgcctcga
     3121 agatccgcct gacgttggcg cgcacctgat cggggccggt ggacacaccc atccggagct
     3181 cgctcggcac cagcacgtcg ggagggaggc cggtgatgtc cccgcccccg cctgggcagg
     3241 tcacatacgc tccggcgcac atcatccgag gcccggcgag cctgccctcc tcgatcgcat
     3301 cgcggagcgc gaggtccacg aacgcgtgga acgtccccac gtcgcggacg gtggtgaagc
     3361 caccgagcag ggtggcgcgg gcgttcgtga cgcccgtgag ggcctcctgc gtggaggagc
     3421 gcgtcacgag gtagccgtac ccctggccgt cgtccagctc cccgatcaga tgggcgtgac
     3481 aatcgatcat gccgggcagg accgtccgtc cggacagatc gatcgtccga ccccgtggtg
     3541 ccgcacccgc ctcgaccacg tcgcggattc gatctcggtc gagcacgaga gcccgatcgc
     3601 tgagcacctc gccggtcgtc acgtcgagca gatggcctgc gagcaccacg agcggttcgg
     3661 ccgatggcat cggcggagca tactctcggc ctcccccagc cgggggaggc ctcgcgacct
     3721 tcgccgggtg cgcggatcga tccgagatgc cgatacccga gacatggtac ggcgcaagca
     3781 ccatgcttcg atcgtggcgg ggtcagcgct gctgatcctc gcctcgctgt tcggcgccgt
     3841 cagcctgccc gccgcggccg tcgaaccctc ctggttcgac ctgccggcgt cggactcctt
     3901 cccgcaggga ctcgccgcgg gaccggacgg gaagacgtgg gtggccagcc gcgcggccgc
     3961 cgagatcgcg cgggtcgctc ccgacggatc gtcgtcgtcg gtccccctcg agtccggcgt
     4021 cgatccctac gacatcgctc gtggatccga cggggcgatg tggttcaccg agcacaacgg
     4081 gaaccggatc gggcggctca cgatcgacgg ggagctctcc gagttctacc tccgcgacct
     4141 gtcgacgccg accggcatca cgctcggccc ggacggcgcg atctggttcg cgcagcgagg
     4201 cgtcggcgcg atcggccgca tcactcccga cggagagttc accgagtggc gaacgatcac
     4261 ccctcgggcc gcgccgctca gcgtgacgac gggatccgac ggggcgatct ggttcacgct
     4321 cacggccgcg aaccggatcg ggcggatcgc gctcgacggc acgatgacgg agttcccgct
     4381 ccccgcgaac gcgagcggcc cgcagtggat caccagcggt ccggacggcg cgctctggtt
     4441 cacggctcgc gcctccaacg cgatcgtgcg gatgacgatc ggcggcgccg tgacgacgta
     4501 cccggtcccc acgccggcgg caggggtgaa cgggatcacg gtcggggccg acggggcgat
     4561 ctggttcacc gagagcaccg ccgacgccgt cggccggatc ggcctcgacg gtgcgatcgt
     4621 cgagatccct ctcggcgagg gcgccacccc gaccggcatc acgaccgggg ccgacggcgc
     4681 gatctggttc agcgcccagg gcacgaaccg cgtggggcgg ctcgagccgt ccggccccgc
     4741 cgacagagcc gctccgaccg tcacgatcgt gtcgccggca cgcggttcgg tgctgaccga
     4801 cggcgagggc atggtcgccg actacttctg caccgatgaa gccgggggct cgggcgtg
//
LOCUS       DASUVE010000199         8781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1227822, whole genome shotgun sequence.
ACCESSION   DASUVE010000199 DASUVE010000000
VERSION     DASUVE010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227822"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VF036_04275"
     CDS             complement(<1..1011)
                     /locus_tag="VF036_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264478.1"
                     /translation="MSATVASDPATRIARWPWVVLGLGAVFASVGLVLVSLNGRALTG
                     EVTNFVAFMGMGVVGALSLSRAPRNVIGLLMLWVAVSVGFAFAMSELSAYLYVRGDLE
                     AAIWVGWPGTTVWALGLFPLLVLLPLLFPDGRLHSRRWRPVLRAGLGVSGIAFFIIGF
                     AAPTQVITIAGRDVEMRNPLAIPGLEQLGALGWVLWPALLALFVLSVVSMVVRFRRSR
                     GVERQQLKWFAFAATILVVYFLASAVLEALEISEGSVGQLISGLAFAGIPVAVGVAIL
                     QYRLWDLDVVVKKTVVAGSLVLLAIAVYAGAVALIGLLAIDQDRPAVLFAIALGLGIA
                     FRP"
     gene            complement(1008..2282)
                     /locus_tag="VF036_04280"
     CDS             complement(1008..2282)
                     /locus_tag="VF036_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6264479.1"
                     /translation="MVLGEDNVLVREGVRALLNSYDDIEVVGVAEDAPTLLATAGDHD
                     PDVVVTDIKMPPSFQLEGIDCAHAIRERHPDTGVVVLSAHDDEEYAIALLGKGHSGLA
                     YLLKDRIAQGDELARAIREVRQGGSVVDPSIAERLSGRAESEEEDRIVLDMMAQGLGY
                     EAMAAALGTTQEAVDGRVTALFRRMAGDDGAGTGLLGEFKRLHAAVVEQSATATSLKS
                     YVPSQFVDALARGGDAASQRELEVTVLFSDIRGFSTIAERLTARDIAEIVGRHLGAMA
                     EVISEHGGTIDKFQGDAVMAVFGAPEPTPDHAERALRCALAMQDRQRELNSMGWGTDA
                     VDGLHVGIGLNTGVVMAGAVGGGGRLEYTVIGDAVNVAQRLQSEAGGGEVVAAASTVS
                     AAPTVAAESIGPRVVKGREEPVEVFLIVGGPA"
     gene            2345..3010
                     /locus_tag="VF036_04285"
     CDS             2345..3010
                     /locus_tag="VF036_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6264480.1"
                     /translation="MPLRVVFAEDNYLVREGTAALLATAEEVDLVGTAVSLDELLSAV
                     DEHAPDAVLTDIRMPPSGTDEGIRGAKQIRSTHPEIGVVVLSQFAEEEYAYDLLKDGA
                     AGLGYLLKERVADVEELVRALTEVARGGSVLDPKIVEALVAAKEHLAHSPLARLTDRE
                     REVLGHMAQGQNNAAIAKSLFLTERAVEKHINSVFHKLDLTEEPDVHRRVMAVLAFLR
                     DQS"
     gene            complement(3040..3447)
                     /locus_tag="VF036_04290"
     CDS             complement(3040..3447)
                     /locus_tag="VF036_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6264481.1"
                     /translation="MATILVGTDTSAAADLAVEDAARLARDRGAELLVLYVKPEGADR
                     SVVDPDRAADPSAHLRALASRFPGVATTARIEDGDAAARICDVAEEVRADTIVLGNRG
                     MHGSRWRVRESVPNLVLRHAPCSVYIVDTRKAQ"
     gene            3625..5667
                     /locus_tag="VF036_04295"
     CDS             3625..5667
                     /locus_tag="VF036_04295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6264482.1"
                     /translation="MSEARPASRLPWILGALTVVTLVASIPFLVLGLDTPLPETAFGF
                     RGWGLVLGSALLVAGVLIATRVPANPIGWILVASGLGTAVQEFGQQYAHYGLLDSPGA
                     VTGADFAAWITEWIWIPYMAAVALLIPMLYPTGRLPSARWRVLLLLGLIAAGIGTVCF
                     ALAPGDLDGFPGVPNPVELDGLPWIKTLGDTVMFVFVGGVLAAIVSMAVRFRRSEGDE
                     RQQLKWLLLAISLLASSFIIGVPYWTLGGNGGSLDVVENVLVAALGAIPVAIGVAILK
                     YRLYDIDVVIKKTVVYAILAAFITAIYLAIVVGIGTLVGSGGNVFLSAAAAAAVAVAF
                     QPARRRAQRVADRLVYGERATPYEVLSGFSERLAETYSVDDVLPRMARVLAEGVGAAT
                     VTIVLGTESSRNVVARWPLDHPPTRSEGRSFEVRHQGQPLGAIEIATAPGEDLDPTRE
                     KLVADVAAQAGLVLRNAALIEDLRASRQRLVTAQDQERRRIERNIHDGAQQQLVALAV
                     KLKLADSLVGTDEARAHQMLADLQTDAGQTLEDLRDLARGIYPPLLADKGLGAALESQ
                     ARKSLVPVSVETDGIGRYPQEIESAVYFSCLEALQNVSKYAEATEATVRLAQSNGLLT
                     FEVHDDGAGFDPSSASRGSGLQGIVDRLAALGGEVEIRSAPGDGTTLGGCVPVEVG"
     gene            5703..7757
                     /locus_tag="VF036_04300"
     CDS             5703..7757
                     /locus_tag="VF036_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6264483.1"
                     /translation="MTLVMLTVWPFLGAASGQLDDELIIYLLAPVAILAYGTVGALIT
                     SRHPENPIGPILLGVGLAFAVSVTAGDYVTLAARSQELLPLTRWVAWLGRVAFAFWLA
                     PLPLLFLLYPDGRLASRRWRPVLWVLLAALGVNLILFALTPGVLAVGFGERNARIANP
                     VGLPIEWLTVVEDATELAGLVAFACAGLAIVSLVLRFRRSTGAERQQMRWLGYMGAVL
                     IAISLLALVNALVRSAIGLAVEDENDLIGITAWVIFFSVLLLGIPAACAIAILRYRLY
                     EIDVVIKKTIVFGILVVLIMVAALGLVLVASGPLSDVVADDALAAGLVGGVLGLLVWP
                     LFRIARRVADRLVYRGRSSSYEILTEFSGRMSVAYGTEDVLPRLASVLGEGTGATRVV
                     VWLRVGRDLRPEAVWPLGTDAPSSPPDDATPVAHQGEVLGALSVELPANDPMNPKKAK
                     IVRDLAAQAGLVLRNVALIEDLRAASQRLVAAQDEERRRIERNIHDGAQQDLVALAVK
                     HRLAASLVGKDEDKLRSMLEQLQSDTNDALENLRDLARGIYPPLLADRGLVAALDAQT
                     RKSSVPTRLEAAEIGRYPQEAEAAVYFSCLEALQNVAKYAGATSATVRLEQSNGSLTF
                     AVSDDGVGFDPAAAERGSGLQGIADRLAALGGTMDVRSTPGHGTTVAGRLPVATAAVD
                     VS"
     gene            7754..>8781
                     /locus_tag="VF036_04305"
     CDS             7754..>8781
                     /locus_tag="VF036_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264484.1"
                     /translation="MSARAARIVAIAIVVLSLISVIASIPLTLASRARIEPGHIVVVG
                     DRASARTQETLEQVRAERDAGDPLTETTGGFTFWTGVIILALFGWLSVGALIVSRQPR
                     NWAGWLFLIIGTPLPLLAITQQLVIYGTKVEPGSVPLLHLWALFGEYGIYPVALLPLL
                     FLLYPDGHPPSPRWRWVSAGLVGGTAFAVLGFLFRPGPFNAYAADGILYVNPLGIDAL
                     AEVGTTMITIGAVVALASAFATVIAVLLRFRRSRGEERQQMRWLAYVASLAGAFFALQ
                     WVLGLTAELFSRDADAPVFEVFFVLTAFTIVFGVPAAYLVAIFRHGLWDLDVVVKKTI
                     QYAVLAVA"
BASE COUNT         1244 a         3122 c         3015 g         1400 t
ORIGIN      
        1 cggccggaac gcgatcccga gccccagcgc gatcgcgaac agcaccgccg gacggtcctg
       61 gtcgatcgcc aacagcccga tcaacgccac ggcacccgcg tacaccgcga tcgcgagcag
      121 gacgagcgag cccgccacga cggtcttctt cacgacgacg tcgaggtccc acagccggta
      181 ctgcaggatc gcgacgccga ccgccaccgg gatccctgcg aacgcgagac ccgagatcag
      241 ctggccgacg ctgccctcgg agatctcgag cgcctcgagc acggcgctcg cgaggaagta
      301 cacgaccaag atcgtggcgg cgaacgcgaa ccacttgagc tgctgccgct ccaccccccg
      361 cgagcggcgg aagcggacga ccatcgacac cacggagagg acgaacagcg ccaggagggc
      421 gggccagagg acccacccga gcgcgccgag ctgctcgagc cccgggatcg ccagcgggtt
      481 ccgcatctcg acgtcgcgcc ccgcgatcgt gatgacctgc gtcggcgcgg cgaagccgat
      541 gatgaagaac gcgatcccgc tcacaccgag gcccgcccgg agcaccggcc gccatctgcg
      601 cgagtgcaat cgtccgtccg ggaagagcag cggcagcagg acgaggaggg ggaacagccc
      661 caacgcccag acggtggtac ccggccaccc cacccagatc gctgcctcca gatcgccgcg
      721 tacgtacagg tatgcgctca gctccgacat cgcgaacgcg aaccccacgc tgaccgcgac
      781 ccagagcatc agcagcccga tcacgttgcg aggggctcgg ctgaggctca gcgcgcccac
      841 gacccccatc cccatgaacg cgacgaagtt cgtgacttcc ccggtcagcg ctcgtccgtt
      901 cagggagacg aggaccaggc cgacggaggc gaacacggcc ccgaggccga gcacgaccca
      961 cggccagcga gcgatccgcg tcgcggggtc gctcgccacc gtcgcgctca cgccggacca
     1021 cccacgatca ggaagacctc caccggctcc tcgcgcccct tgaccacgcg cgggccgatc
     1081 gattcggcgg cgacggtcgg cgctgccgag acggtcgagg ccgcggccac gacctcgccg
     1141 cccccggctt cagactgcag ccgctgcgcg acgttcacgg cgtcaccgat caccgtgtac
     1201 tcgagccggc cgccgccgcc gacggccccc gccatcacga cgcccgtgtt cagaccgatg
     1261 cccacgtgga gtccgtcgac agcgtcggtc ccccacccca tcgagttcag ctcccgctga
     1321 cggtcctgca tcgccagcgc gcagcggagc gcccgctccg cgtgatccgg cgtgggctcc
     1381 ggcgccccga agaccgccat cacggcgtca ccctggaact tgtcgatcgt gccgccgtgc
     1441 tcggagatca cctcggccat cgcgccgaga tgacgcccca cgatctccgc gatgtcgcgc
     1501 gccgtgaggc gctcggcgat cgtggagaac ccgcggatgt cgctgaacag gacggtcacc
     1561 tcgagctcgc gctggctcgc ggcgtcgccg ccgcgcgcca gcgcgtccac gaactgcgaa
     1621 ggcacgtacg acttgagcga cgtcgccgtg gcggactgct ccaccaccgc cgcgtggagc
     1681 ctcttgaact ccccgagcag gccggtgccg gccccgtcgt cgccggccat ccgccggaac
     1741 agcgccgtca cgcgtccgtc gaccgcctcc tgggtggtgc cgagcgcggc cgccatcgcc
     1801 tcgtacccga gcccttgcgc catcatgtcg aggacgatcc ggtcctcctc ctctgattcc
     1861 gcgcgtcccg acaggcgttc ggcgatcgac gggtcgacca cgctcccgcc ctggcggacc
     1921 tcccggatcg cacgggcgag ctcgtcccct tgcgcgatcc ggtccttcaa gaggtaggcg
     1981 agtccactgt gccccttgcc gagcagcgcg atcgcgtact cctcgtcgtc gtgcgccgag
     2041 aggacgacga cgccggtgtc ggggtggcgc tcccggatcg cgtgtgcgca gtcgatgccc
     2101 tcgagctgga aggacggcgg catcttgatg tccgtgacga cgacgtccgg gtcgtgatca
     2161 cctgccgtcg cgaggagcgt gggcgcatcc tccgcgaccc cgaccacctc gatgtcgtcg
     2221 taggagttca ggagcgcccg cacaccttcg cgcacgagca cgttgtcctc gccgagtacc
     2281 acccggatca tcacgtaacc gccccctgaa cactccgcga gttcctcgcg tgtaccatcc
     2341 gcgcatgccg ctgcgggtgg tcttcgcgga ggataactac ctcgtgcggg agggcaccgc
     2401 cgcgctcctc gcgacggctg aggaggtcga tctcgtggga accgccgtga gcctcgacga
     2461 gctgctgtcc gccgtcgacg agcacgcccc cgacgccgtc ctcacggaca tccgcatgcc
     2521 gccgagcggc accgacgaag ggatccgcgg cgcgaagcag atccgttcca cccaccccga
     2581 gatcggggtc gtggtgctgt cgcagttcgc ggaggaggag tacgcctacg acctgctgaa
     2641 ggacggcgcg gccggactgg gctacctgct gaaggaacgc gtggccgacg tcgaggagct
     2701 cgtgcgggcg ctcacggagg tcgcgcgggg cgggagcgtg ctcgatccga agatcgtgga
     2761 ggcgctcgtc gcggcgaagg agcacctcgc gcactccccg ctcgcgcggt tgaccgaccg
     2821 ggagcgcgag gtgctcggcc acatggcaca ggggcagaac aacgcggcca tcgcgaagtc
     2881 gctgttcctc accgagcgcg ccgtcgagaa gcacatcaac tccgtgttcc acaagttgga
     2941 tctgaccgag gagcccgacg tgcatcgccg cgtgatggcg gtgctcgcgt tcctgcgcga
     3001 tcagagctag cgccggctcg cgtcgcgtcc ggcccatcct cattgcgcct tgcgggtgtc
     3061 gacgatgtag acgctgcacg gggcatgccg gagcacgagg ttcgggacgc tctcgcgcac
     3121 acgccaccgc gagccgtgca tcccccggtt cccgaggacg atggtgtccg ccctgacctc
     3181 ctccgcgacg tcacagatgc gggcggccgc gtcgccatcc tcgatccgcg ccgtcgtcgc
     3241 cacgccgggg aaccgggacg cgagagcccg gaggtgcgcg gacggatccg cggctcgatc
     3301 cggatccacg accgaccggt cggctccctc cggcttcacg tacaggacca ggagctcggc
     3361 gccgcggtcg cgcgcgagtc gcgccgcgtc ctcgaccgcg aggtcggcgg ccgccgaggt
     3421 gtcggttcca acgaggatcg tcgccatctg aggtcctcct tcgttcgtcg ccccgacgct
     3481 acgccgcggc cccggggtcc gacgacggcg ctcgcatgcc gacttcgccc gggtgccagc
     3541 accccctgac gccgggtgtt ctcgcatcgg aaaccgcagg tcgacgccct tggagccggc
     3601 cgctgccgat gggagcatcc gggcatgagc gaagcgcggc ccgcctcgcg gctcccctgg
     3661 atcctcggcg cgctcacggt ggtgaccctg gtggcgtcga tcccgttcct cgttctgggg
     3721 ctcgacacgc cgctgcccga gacggcgttc gggttccgag ggtggggact ggtcctcggg
     3781 tcggcgttgc tcgtggccgg cgtcctgatc gcgacgcgtg tccccgcgaa cccgatcggc
     3841 tggatcttgg tcgcgtccgg cctcgggacc gcggtacagg agttcggtca gcagtacgcg
     3901 cattacgggc tgctcgactc ccccggcgcc gtcaccggcg cggacttcgc tgcgtggatc
     3961 accgaatgga tctggatccc gtacatggcg gcggtcgcgc tgctgatccc gatgctgtat
     4021 cccacgggcc ggctcccgtc cgcgcgctgg cgggtcctgc tcctcctcgg gctgatcgcg
     4081 gcggggatcg gcaccgtctg cttcgcgctc gcacccggcg acctcgacgg cttccccggc
     4141 gtaccgaacc ccgtggagct cgacggcttg ccgtggatca agacgctcgg ggacaccgtg
     4201 atgttcgtgt tcgtgggagg tgtcttggcg gcgatcgtgt cgatggcggt ccggttccgc
     4261 cggtccgagg gcgacgaacg acagcagctg aagtggctcc tgctcgcgat ctcgctgctg
     4321 gcctcgagct tcatcatcgg cgtcccgtac tggaccctcg gagggaacgg ggggtcgctg
     4381 gacgtcgtgg agaacgtgct cgtggccgcg ctcggggcga tcccggtagc gatcggtgtc
     4441 gcgatcctca agtaccggct ctacgacatc gacgtcgtga tcaagaagac cgtggtctac
     4501 gcgatcctcg cggccttcat cactgcgatc tacctggcga tcgtcgtcgg catcggcacg
     4561 ctcgtcggga gcggcgggaa cgtgttcctc tccgccgcgg cggctgccgc ggtcgcggtg
     4621 gcgttccagc ccgcccggcg gcgtgcccaa cgggtcgccg accgtctggt ctacggggaa
     4681 cgggcgacgc cgtacgaggt gctctccggc ttctccgagc gcctggccga gacgtattcc
     4741 gtcgacgacg tccttccgcg gatggcgcgc gtgctcgccg aaggcgtcgg ggccgcaacc
     4801 gtgacgatcg tcctgggaac ggagagctcg cggaacgtcg tggcgcgatg gccgctcgat
     4861 cacccgccca cgcgatccga gggacggtcc ttcgaggtgc ggcaccaagg acagccgctc
     4921 ggtgcgatcg agatcgcgac cgcgccgggg gaggacctcg accccacacg ggagaagctc
     4981 gtcgccgacg ttgcggccca ggcgggactc gtgctccgca acgcggcgtt gatcgaagat
     5041 ctgcgggctt cgcgacaacg cctcgtcacg gcgcaggatc aggagcgtcg aaggatcgaa
     5101 cggaacatcc acgacggcgc gcagcaacag ctggtcgcgc tcgccgtcaa gctgaagctc
     5161 gcggactcgc tcgtcggcac ggacgaggcc cgggctcacc agatgctcgc ggatctccag
     5221 acggatgccg gtcagacgct ggaggacctg cgcgacctcg cacgggggat ctacccgccg
     5281 ctcctcgccg acaaggggct cggcgcggcg ctggagagtc aggcgcgcaa gtcgctcgtt
     5341 ccggtctccg tcgaaaccga cgggatcggc cgctatccgc aagagatcga atccgccgtg
     5401 tacttctcgt gtctggaggc gttgcagaac gtgtcgaagt acgccgaggc gacggaggcg
     5461 accgtgcggc tcgcgcagtc gaacggcctg ctcacgttcg aggtccatga cgacggcgca
     5521 ggtttcgacc cgtcgagcgc ctcgcgcggc tccgggctcc aggggatcgt cgaccgtctc
     5581 gccgcgctcg gcggcgaggt cgagatccga tcggcgcccg gcgacggcac gaccctcggg
     5641 gggtgcgtcc cggtggaggt cgggtgatga tggctcggcg cttggcttgg ggcgcatggg
     5701 gcatgaccct ggtcatgctc acggtctggc cgttcctcgg ggcagccagc gggcaactcg
     5761 acgacgagct gatcatctac ctcttggcgc cggtcgcgat cctcgcgtat gggaccgtcg
     5821 gcgccctgat cacctcgcgg caccccgaga acccgatcgg accgatcctg ctcggcgtcg
     5881 gtctcgcctt cgcggtctcc gtcacggcgg gcgactacgt caccctggcc gcgcgcagcc
     5941 aggagctcct cccgctgacc cgttgggtcg cctggctggg ccgggtcgcg ttcgcgttct
     6001 ggctggcgcc gctcccgctc ctgttcctgc tctatccgga tggacggctc gcgagtcgtc
     6061 gctggcgtcc ggtgctctgg gtcctcctcg cggccctcgg cgtcaacctg atcctcttcg
     6121 cgctgactcc cggggtgctc gcggtgggct tcggcgagcg caacgcgagg atcgcgaatc
     6181 ccgtcggcct gccgatcgag tggctcactg tggtcgagga cgcgacggag ctcgccggtc
     6241 tcgtcgcctt cgcgtgtgcg ggtctggcta tcgtctcgct cgtcctccga ttccgcagat
     6301 cgaccggtgc ggagcgccag cagatgcggt ggctcgggta catgggagcg gtgctcatcg
     6361 cgatctccct cctcgcgctg gtcaacgcgc tggtcaggag cgcgatcggt ctagccgtgg
     6421 aggacgagaa cgacctcatc ggcatcacgg cgtgggtgat cttcttctcc gtgctgctct
     6481 tgggtatccc tgccgcctgt gcgatcgcga tcctccggta tcgcctctac gagatcgacg
     6541 tcgtgatcaa gaagacgatc gtgttcggga tcctggtggt gctgatcatg gtcgccgcgc
     6601 tcggtctggt cctcgtggcg agcggtccgc tgagtgacgt ggtcgcggac gacgcgctgg
     6661 cggcgggtct cgtcggcggc gtcctcgggt tgctcgtgtg gccgctgttc aggatcgctc
     6721 gacgcgtcgc cgaccgactc gtctaccgag gtcggtcgag ctcctacgag atcctcaccg
     6781 agttctccgg gcgcatgtcc gtcgcctacg gcaccgagga cgtccttccc cgtctggcct
     6841 ccgtcctcgg cgaagggacg ggtgccacgc gcgtcgtcgt ttggctgcgc gtcggtcggg
     6901 acctccgacc cgaggccgtc tggccgttgg gtacggacgc accgagcagc ccgcccgacg
     6961 atgcgactcc ggttgcccac caaggggagg tgctcggagc gctctcggtc gagttgcccg
     7021 cgaacgatcc gatgaacccc aagaaagcga agatcgtccg tgaccttgct gcccaggcgg
     7081 gtctcgtgct tcggaacgtc gccctgatcg aggacctgcg cgcggcgagc cagcgactgg
     7141 tggctgctca ggacgaggag cgtcgacgca tcgagcggaa catccacgat ggcgcccagc
     7201 aggacctggt ggcactggcc gtgaagcacc gcctcgccgc gtccctggtc ggcaaggacg
     7261 aggacaagct ccgctcgatg ctcgagcagc tccagtcgga caccaacgat gcgctggaga
     7321 acctccggga tctcgcacga ggcatctacc cgccgctgct cgccgaccgg ggtctcgtcg
     7381 cggcgctcga cgcgcagact cggaaatcct cggtccccac tcgtctggag gcggccgaga
     7441 tcggtcgcta cccgcaggaa gccgaggcgg ccgtgtactt ctcctgtctc gaggcgctcc
     7501 agaacgtcgc gaagtacgcg ggggcgacga gcgccaccgt tcggctcgaa cagtccaacg
     7561 gcagcttgac cttcgcggtc tcggacgatg gcgtgggctt cgacccagcg gcggccgagc
     7621 gcggatcggg gctccagggg atcgccgacc ggctcgccgc gctcggcggc accatggacg
     7681 ttcgctcgac gcccggtcac ggaacgaccg tggccggacg cctccccgtg gcgacggcgg
     7741 cggtggacgt gtcgtgagcg ctcgggcggc tcggatcgtc gcgattgcga tcgtcgtcct
     7801 cagcctcatc tcggttatcg cgtcgatccc gctgacactc gcgtcgcgcg ctcggatcga
     7861 gcccgggcac atcgtcgtcg tcggcgacag ggcgtccgcg cggacgcagg agaccctcga
     7921 gcaggtccgg gcggagcgcg acgcgggcga ccccctgacc gagacgacgg ggggattcac
     7981 gttctggaca ggggtgatca tcctcgcgct gttcggctgg ctcagcgtcg gagcgctgat
     8041 cgtgtcgcgc cagccgcgga actgggcggg atggctcttc ctgatcatcg gaactccgct
     8101 gccgctgctg gcgatcaccc aacagttggt gatctacggg acgaaggtcg agcctggctc
     8161 ggttccgctg ctccatctct gggcgctctt cggcgagtac gggatctatc ccgtcgcgct
     8221 gctgccgctc ctgttcctcc tgtacccgga cgggcacccc cccagccccc ggtggcgatg
     8281 ggtgagcgcc gggctggtcg gcggcacggc gttcgcggtc ctggggttcc tgttccgtcc
     8341 cgggccgttc aacgcgtacg cagcggatgg gatcctgtac gtgaacccgc tcgggatcga
     8401 cgccctggcg gaggtcggga cgacgatgat cacgatcggc gcggtggtcg cgctcgcgtc
     8461 agcgttcgcg accgtgatcg cggtcctcct gcggttccgg cggtcgcggg gcgaggagcg
     8521 acagcagatg cgttggctcg cgtacgtcgc ctcgctcgcc ggcgccttct tcgccctgca
     8581 gtgggtgctt ggcctcacgg ccgaactgtt cagccgggac gcggatgcgc cggtcttcga
     8641 ggtgttcttc gtcctgaccg cgttcacgat cgtgttcggc gttccggccg cctacctcgt
     8701 ggcgatcttc cgtcacggac tctgggatct ggacgtcgtc gtgaagaaga cgatccagta
     8761 cgcggtgctc gctgttgcgt t
//
LOCUS       DASUVE010000200         4004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_475979, whole genome shotgun sequence.
ACCESSION   DASUVE010000200 DASUVE010000000
VERSION     DASUVE010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475979"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..985
                     /locus_tag="VF036_04310"
     CDS             59..985
                     /locus_tag="VF036_04310"
                     /inference="COORDINATES: protein motif:HMM:NF044544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264485.1"
                     /translation="MKAPPVTLLWGEDDFLVREAALALLGDTKATAVDAAAWQGGELQ
                     GLATPSLFGEPRALLVNDARSLRKETLDEIGRYLTEPDPDATLVLACVVADRGKVPAA
                     LQKLVEPLGEIRKVDIARRDLEPWIVGRAKEHGLDLAIPGARALVETLGPEPGQLAAA
                     LGQLQDAFPGQRVGPREIAGQFRGLGEQKTWDLCDRAFGKDLPGAIRSLRSIEEGGDE
                     AIMVLGGIAARLRDLLKVRSLPDRMPPAQVAREAGLRFDWQARRYQQQARNFSMEQLV
                     ELHDRVTEADRALKSGSTGDVVMPTLVTAIAG"
     gene            complement(982..1596)
                     /locus_tag="VF036_04315"
     CDS             complement(982..1596)
                     /locus_tag="VF036_04315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6264486.1"
                     /translation="MAGYPLDRSIESYYERRSAEYDDWYLGSGLFAERDRPGWNEDVR
                     AVIEAVSSLSAARTVDVACGTGFLTQHLRGRVVGVDASAGMLSASRARHAHPLVRADG
                     FGLPFGDASFDRVFTGHFYGHLVPEQRGRFLVESRRVAPELVVVDAKIHPDVRTEQMQ
                     ERVLNDGSSHRVFKRYFTAGQLADELGGGQVLFDGRWFVVVASP"
     gene            complement(1636..1905)
                     /gene="rpsT"
                     /locus_tag="VF036_04320"
     CDS             complement(1636..1905)
                     /gene="rpsT"
                     /locus_tag="VF036_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX6264487.1"
                     /translation="MANIKSQVKRNKQNEKRAERNKAVRTALKTSTKKIRTAVDTGDA
                     EEAQARQRETARSLDKAVAKGIVHKRTAARRKSRLAKAVSSVGAE"
     gene            complement(1930..3648)
                     /locus_tag="VF036_04325"
     CDS             complement(1930..3648)
                     /locus_tag="VF036_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX6264488.1"
                     /translation="MRYVALACDYDGTIAEDGKVPDRVVEAIQRVRESGRHALLVTGR
                     ELEDLRGAFDRLDVFDRVVAENGALLLDPETRDVRQLAEPPPEAFVERLRERGVEPLS
                     VGQVIVATWEPHQEAVLEAIHDLGLELQVIFNKGAVMVLPPLVNKASGLRAALTDLSL
                     SPHNVVGVGDAENDHAFLDLCELSVAVENALPSVKERSDHVTEADHGEGVVQLVDTLL
                     DDDLENLDERVRRHDLAIGTRDDDEPVTVRPYRTNVLITGPSGSGKSTLTTALLEQLK
                     EHGYQFLLVDPEGDYDALEDVVVLGSPDRVPTVEEVLDVLAEPGSSLAANLLGVPLGD
                     RPAYLESLLPRLQELRAKTGRPHWMVVDEAHHLLPSGLQTAGTSMPTDVSSMMLVTVH
                     ADAMTPEALRPVNVVIVPAKGARESLDVFAETVEMDSPETEDLDPGKDEAVAWFVPDE
                     PLVFRPLEPTQDLKRHRRKYAEGDLEEDTFVFRGPEGRLSLRAQNLQLFSQIAEGVDD
                     ETWVWHLERGDYERWFREAIGDDTLADLAKESADAETPDESRNLILEAIEERYTLPAE
                     STGKRP"
     gene            3705..>4004
                     /locus_tag="VF036_04330"
     CDS             3705..>4004
                     /locus_tag="VF036_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX6264489.1"
                     /translation="MDVSHIRNFCIVAHIDHGKSTLADRLLELTHAVADRDMRAQYLD
                     RMDLERERGITIKAQAVRLTHGGYELNLIDTPGHVDFTYEVSRSLEACEGAILLVD"
BASE COUNT          626 a         1392 c         1353 g          633 t
ORIGIN      
        1 gcacgatcac ggtcgtcttc gcaggtccga acccgacggt agagttcgag cgatggggat
       61 gaaggcgcct cccgtgacgc tcttgtgggg agaggacgac ttcctggttc gcgaggcggc
      121 gctcgcgctc ctcggcgaca ccaaggcgac ggcggtcgac gccgccgcgt ggcagggcgg
      181 tgagctccag ggtcttgcga cgccgtcgct cttcggcgaa ccgcgggcgc tcctcgtgaa
      241 cgacgcccga tcgctccgga aggagaccct cgacgagatc gggcgttacc tcaccgagcc
      301 ggatccggac gccacgctcg ttctcgcgtg cgtcgtcgcc gatcgcggga aggtcccggc
      361 ggccctccag aagctggtgg aaccgctcgg cgagatccgg aaggtggaca tcgcccgcag
      421 ggacctcgag ccgtggatcg tcggccgggc caaggagcac ggcctcgacc tcgcgatccc
      481 gggggcccgc gcgctcgtcg agacgctcgg gcccgagccc ggtcagctcg cggcggcgct
      541 cggccagctc caggacgcgt tccccgggca gcgcgtcggc ccgcgggaga tcgcggggca
      601 attccgcggg ctcggcgagc agaagacgtg ggacctctgc gaccgggcgt tcgggaagga
      661 cctccccggc gcgatccgct cccttcggtc gatcgaggag ggtggcgacg aggcgatcat
      721 ggtcctcggc gggatcgccg cgcgcctgcg cgacctcctc aaggtgcgat cgctcccgga
      781 ccgcatgccg ccggcgcagg tggcgaggga ggccgggctt cgtttcgact ggcaggcgcg
      841 ccgctaccag cagcaggcga ggaacttctc gatggagcag ctcgtggagt tacacgaccg
      901 cgtcaccgag gccgaccgcg cgctgaagtc cggctccacc ggcgacgtcg tgatgcccac
      961 gctcgtcacc gcgatcgccg gctagggcga cgccaccacc acgaaccagc gcccatcgaa
     1021 cagcacctgg cctccaccga gctcgtcggc gagctgtccg gccgtgaagt accgcttgaa
     1081 cacgcgatgc gacgacccgt cgttcaggac gcgctcctgc atctgctcgg tccgaacgtc
     1141 gggatggatc ttcgcgtcga cgacgacgag ctccggcgcc acccgccggc tctcgaccag
     1201 gaaccgccca cgttgctccg ggacgaggtg cccgtagaag tgacccgtga agacgcgatc
     1261 gaaggaggca tctccgaacg gcagcccgaa cccgtcggca cgaacgagcg gatgtgcatg
     1321 tctggcgcgc gacgccgaca gcatcccggc gctcgcatcg acccccacaa cccggccgcg
     1381 gagatgctgc gtgaggaagc ccgtcccgca cgccacgtca accgtgcggg ccgccgacaa
     1441 ggacgacacg gcctcgatca ccgcgcggac gtcctcgttc cacccggggc gatctcgttc
     1501 cgcgaagagc ccggatccca ggtaccagtc gtcgtactcg gccgagcggc gctcgtagta
     1561 cgactcgatc gagcggtcga ggggataccc agccatcgac cgacagtgta gggacggggc
     1621 gactgccggg aggaactact cggcgccgac ggagctgacg gccttcgcga ggcggctctt
     1681 gcgccgggcg gcggtgcgct tgtggacgat gcccttcgcc acggccttgt cgagggaccg
     1741 cgcggtctcc cgttgccggg cctgggcctc ctcggcatcg cctgtgtcga cggccgtgcg
     1801 gatcttcttc gtcgacgtct tgagggcggt gcggacggcc ttgttgcgct cggcgcgctt
     1861 ctcgttctgc ttgttccgct tgacctgcga cttgatgttg gccatgagcg ggggagtcta
     1921 ccagcgggtt cagggccgct tgcccgtgga ctccgcagga agcgtgtacc gctcctcgat
     1981 cgcctcgagg atcaggttac ggctctcatc cggcgtctct gcatcggccg actccttcgc
     2041 cagatcggca agcgtgtcgt cgccgatggc ctcccggaac caccgctcgt agtccccgcg
     2101 ctcgagatgc cagacccacg tctcgtcgtc gacgccctcg gcgatctgcg agaagagttg
     2161 gaggttctgg gcccgaaggc tgagtcttcc ctcgggacca cggaagacga acgtgtcctc
     2221 ctcgagatct ccctcggcgt acttcctccg gtggcgcttg aggtcctggg tcggctcgag
     2281 cggtcggaac accaagggct cgtccggcac gaaccaggcc accgcctcgt ccttgcccgg
     2341 gtcgagatct tcggtctccg ggctgtccat ctccacggtt tccgcgaaca catcgagcga
     2401 ctcgcgggca cccttcgccg gcacgatcac cacattgaca ggccgcagcg cctccggagt
     2461 catcgcgtcc gcgtgcaccg tgacgagcat catcgagctg acatcggtcg gcatgctcgt
     2521 tccggcggtc tgcaacccgg acgggagcag atggtgcgcc tcatcgacca ccatccagtg
     2581 gggccgtcca gtcttggcgc ggagctcctg gagtcggggg aggaggctct ccaggtacgc
     2641 aggacgatcg ccgagtggta cgccgaggag gttcgcggcg aggctggatc ccggttccgc
     2701 gagcacatcg aggacctcct ccaccgtcgg cacacgatcg ggggacccga ggacgacgac
     2761 gtcctcgagg gcgtcgtagt cgccttcggg atccacgagg aggaactgat acccgtgctc
     2821 cttgagctgc tcgaggagcg ccgtcgtgag ggtcgacttg ccgctgcccg acgggccggt
     2881 gatcaggacg ttcgtgcggt agggacggac cgtcaccggc tcgtcgtcgt cccgtgtgcc
     2941 gatcgccagg tcgtgtcgcc gaacccgctc gtcgaggttc tcgaggtcgt cgtcgagcag
     3001 ggtatccacc agctggacca cgccctcgcc gtgatcggcc tcggtcacgt ggtccgaacg
     3061 ctccttgacg gaaggcagcg cgttctcgac cgcgacagaa agctcacaga gatcgaggaa
     3121 cgcgtggtcg ttctcggcgt cgccaacgcc gacgacgttg tgcggcgaca ggctgagatc
     3181 ggtgagcgcg gcgcggagac cgctcgcctt gttcacgagc ggtggcagca ccatcacggc
     3241 gcccttgttg aagatcacct ggagttccag cccgaggtcg tggatcgcct cgaggacggc
     3301 ctcctgatgt ggctcccacg ttgcgacgat cacctggcct acggacagcg gctcgacgcc
     3361 tcgctcccgg agccgctcga cgaacgcctc gggaggcggc tccgccagct gccgaacgtc
     3421 gcgcgtctcg gggtcgagga ggagtgcccc gttctcggca acgacccggt cgaacacgtc
     3481 gagacgatcg aaggctccgc gcaggtcctc cagctcccga ccagtgacga ggagagcgtg
     3541 acgtccgctc tcccggacgc gctggatcgc ttcgacgacc cgatcgggga ccttcccgtc
     3601 ctccgcgatc gtcccgtcgt agtcacaggc gagggccacg tagcgcatcg ccgcactgca
     3661 tacccatccg gggaccgcag cacgcagccg taccatgaac gttcgtggac gtctcccaca
     3721 tccggaattt ctgcatcgtg gcgcacatcg accacggaaa gtcgacgctg gccgatcgtc
     3781 tgctggagct cacccatgcc gtcgccgacc gcgacatgcg agcgcagtac ctcgaccgga
     3841 tggacctgga acgggaacgg ggtatcacga tcaaggcgca ggccgtccgg ctcacccacg
     3901 gcggctacga gctgaacctg atcgacaccc ccgggcacgt ggacttcacc tacgaggtct
     3961 cacgttcgct cgaagcctgc gagggggcga tcctcctggt cgac
//
LOCUS       DASUVE010000201         4420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_476127, whole genome shotgun sequence.
ACCESSION   DASUVE010000201 DASUVE010000000
VERSION     DASUVE010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476127"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1812)
                     /locus_tag="VF036_04335"
     CDS             complement(<1..1812)
                     /locus_tag="VF036_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018805281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX6264490.1"
                     /translation="MQALPLLPLTTGVVLPGMVVTLTIESDEARAAVGAAAEGDDALM
                     MTVPRVDGRYAQVGVVAKIEDVGRLRSGVEAVVIRGLRRATVGLGVPGTGEATWVQIG
                     PEPQEPVSERARELARDYRGAVEAITEARGVPAVADVLKGVTDPGQLADMSGYSPDLS
                     FEQKVEVLETLDVEARLKKVLRWAKETLAEVQVRERIRDEVAGSLEERQREMLLREQM
                     AAIRKELGEDGSEDVLEEYRTKIADAGMPEDVRTQADRELGRLERTSEQSPEYGWIRT
                     YLDWLLDVPWSSKTEDNLDIESAREVLDADHEGLRDVKDRILEYLAVRKLRSERGLAE
                     TAGRGSGAILTLVGPPGVGKTSLGESVARALGRNFVRVSLGGVHDESEIRGHRRTYVG
                     ALPGRIVRALKDAGTKNPVMMLDEVDKVGADWRGDPSSALLEVLDPAQNHTFRDNYLD
                     VDLDLSEVLFIATANVAETIAGPLLDRMEVIRIDGYTEDEKVAIARRHLLPRQVERNG
                     LRPEEVEVTDDALRGVVGDFTREAGVRNLERELGKIMRKVATKLTAGEGASPARVDAT
                     DLREYLGRQKFFFEAADRTAVPGVATGLAVTGTGGDVL"
     gene            1979..2989
                     /locus_tag="VF036_04340"
     CDS             1979..2989
                     /locus_tag="VF036_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6264491.1"
                     /translation="MANAIITTNSLTKSFGDVRAVKGVDLSVEEGTVFGLLGPNGAGK
                     TTAIRILTTLLEPDEGTAIVAGFDVVRDAALLRPVIGLAGQFAAVDENLTGLENLEMV
                     GRLYHLPAVEARKRAQEVLERFELSEAAHRTAKTYSGGMRRRLDVGASLVGRPRVLFM
                     DEPTTGLDPRSRLVLWELIRDLQKGGTTLLLCTQYLEEADNLCDRIAVIDLGTVIAEG
                     TSEELKDKIGGEVLELHVAAEHLSRAADTLRGLGAGEISADQGTRLIRVPVGSEGPTV
                     LLESVRKLDDAAIPIEDIALHRPTLDDVFLSLTGHAAEMELEEEAESPEPVKKRRKRR
                     ER"
     gene            2986..3822
                     /locus_tag="VF036_04345"
     CDS             2986..3822
                     /locus_tag="VF036_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6264492.1"
                     /translation="MSAVAVRAPGIGTQAVTSVQDSLVVARRNLMQIVRIPAVLVFEL
                     VQPVMFVLLFVYVFAGNIQNVPPEIEAIGGYVMFLMPGIIIQNAIFGATTTAIGIAED
                     LKAGFVDRFRSLPMARSAMLAGRATSDLIKNFVLLLVVIGIGYLVGFSFENGFLGAIA
                     MIALTMAVSFTFSWVMAWIALSVKKLEAVQAAAFTGIFPLVFMSSAIVPIAGMAPWVQ
                     VVARNNPITHWANLARYFAFGEYSTMGGTPEQLMLWSALWIVGIVAVFIPLSIRLYNK
                     LT"
     gene            3880..>4420
                     /locus_tag="VF036_04350"
     CDS             3880..>4420
                     /locus_tag="VF036_04350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6264493.1"
                     /translation="MPLLGFKGENAREKVIVLLTMCFALAMAMLDNTVVNIALPTITR
                     KLNATVSDLQWIIDGYVLAFASLLLTGGILGDRYGRKKMFLTGLAIFTLASLGCGLSA
                     SGSQLIAFRALQGVGAALLMPGTLSILTVTFPPEERSRAIGIWAGVSGLALALGPTLG
                     GYIVEHSGWETVFFINVPIG"
BASE COUNT          660 a         1542 c         1466 g          752 t
ORIGIN      
        1 cagcacgtcc ccgccggtcc cggtaacggc caatccggtg gccacgccgg ggacggcggt
       61 tcgatccgcc gcctcgaaga agaacttctg ccggccgagg tactcgcgga gatccgtcgc
      121 gtccacgcga gccggcgatg cgccctcgcc ggcggtcagc ttggtcgcga ccttccgcat
      181 gatcttgccg agctctcgct cgaggttgcg gacaccggcc tcgcgcgtga agtcgccgac
      241 cacccctcgc agggcgtcgt cggtcacctc gacctcctcg gggcggaggc cgttgcgctc
      301 gacctgccgc ggcagcaggt gccgccgcgc gatcgcgacc ttctcgtcct cggtgtaccc
      361 gtcgatccgg atgacctcca tccggtcgag cagcgggccc gcgatcgtct ccgcgacgtt
      421 cgcggtcgcg atgaacagca cctcggacag atcgaggtcg acgtccaggt agttgtcgcg
      481 gaacgtgtgg ttctgggcgg gatcgaggac ctcgagcaat gcgctcgagg ggtcgcctcg
      541 ccagtcggcg ccgaccttgt cgacctcgtc cagcatcatc acggggttct tcgtgcccgc
      601 gtccttcagc gcgcgcacga tccgtcccgg cagcgcgccg acgtaggtgc gacggtggcc
      661 acggatctcg gactcgtcgt gaacgccgcc gagcgagacc cggacgaagt tgcgtccgag
      721 ggcgcgcgcg acggactccc cgagcgacgt cttgccgacg cccggagggc cgaccagcgt
      781 gaggatcgcg ccggagccgc gaccggcggt ctcggcgagc ccccgctcgc tccggagctt
      841 gcggaccgcg aggtactcga ggatgcgatc cttcacgtcg cggagcccct cgtggtccgc
      901 gtccaacacc tcgcgcgccg actcgatatc caggttgtcc tcggtcttcg acgaccaggg
      961 gacgtcgagc agccagtcga ggtaggtgcg gatccatccg tattccggcg actgctcgga
     1021 cgtccgctcg agccgcccga gctcgcggtc cgcctgcgtg cgcacgtcct ccggcatccc
     1081 ggcgtccgcg atcttggtcc gatactcctc gaggacgtct tcggaaccgt cctcgccgag
     1141 ctccttgcgg atcgccgcca tctgctcgcg gagcaacatc tcgcgctgcc gctcctcgag
     1201 gctgccggcc acctcgtcgc ggatccgctc gcgcacctgg acctcggcga gcgtctcctt
     1261 cgcccagcga aggaccttct tcagccgcgc ctcgacgtcg agggtctcga ggacctcgac
     1321 cttctgctcg aacgagaggt cgggggagta cccggacatg tcagccagct ggccgggatc
     1381 cgtcacgccc ttcaggacgt cggccacggc cggcacgccg cgcgcctcgg tgatcgcctc
     1441 caccgctccg cggtagtcgc gggcgagctc ccgcgctcgc tcgctaacgg gctcctgggg
     1501 ctccggtccg atctggaccc aggtggcctc gccggtccct gggaccccca ggcccaccgt
     1561 cgcacgccgg aggccacgga tcaccacggc ttcgacgccg ctccggagcc ggccgacgtc
     1621 ctcgatcttc gccacgacgc cgacctgggc gtaccgcccg tccacgcgcg gcacggtcat
     1681 catcagcgca tcgtccccct cggcggccgc gcccaccgcc gcccgagcct cgtccgactc
     1741 gatcgtgagg gtcacgacca tgccggggag caccacgccg gttgtcaggg gcagcaacgg
     1801 cagggcctgg atgtcgagct cgttcatctc ggtgatcctt tctcgctctg tccgcttgaa
     1861 cggacccgcg ctcccatcct attccagtcc ggtaatgatg attacgtgat tgcaccggct
     1921 accagcccgt tacccagcgg acatgtcggt gtcgtcgagc gtcgatatcc tcgcgcccat
     1981 ggcgaatgcg atcatcacca ccaacagcct gaccaagtcc ttcggcgacg ttcgggcggt
     2041 caaaggggtg gatctgtcgg tcgaggaggg cacggtgttc gggctgctcg ggccgaacgg
     2101 tgccggcaag accaccgcca tccggatcct cacaacactc ctcgagcccg acgaaggcac
     2161 ggccatcgtg gctgggttcg atgtggtgcg ggacgcggca ctcctgcggc cggtgatcgg
     2221 tctcgcgggc cagttcgcag ccgtggacga gaacctgacc ggcctcgaga acctcgagat
     2281 ggtcggacgg ctgtaccacc tcccggcggt cgaggctcgc aagcgcgccc aggaggtgct
     2341 cgagcggttc gaactctccg aggccgccca tcgcaccgcc aagacgtact cgggagggat
     2401 gcgccggcgg ctcgacgtcg gcgcgagcct cgtcggtcgc ccgagggtgc tgttcatgga
     2461 tgagccgacc acggggctgg acccgcggag tcgactggtc ctctgggagc tgatccgcga
     2521 cctccagaaa ggggggacca ccctgctgct ctgcacccag tatctggagg aagccgacaa
     2581 cctgtgcgat cggatcgcgg tgatcgacct cgggaccgtg atcgccgagg ggacgtcgga
     2641 ggagctgaag gacaagatcg gcggcgaggt cctcgagctg cacgtcgcgg cagagcacct
     2701 ttcccgtgcg gccgacacgc tccgaggact cggcgcgggt gagatctcgg cggaccaagg
     2761 aacgcgcctg atccgggtgc cggtcggaag cgaaggcccc accgtgctgc tcgagagcgt
     2821 ccgcaagctc gatgacgcgg cgatcccgat cgaggacatc gccctgcacc gtccgacgct
     2881 cgacgacgtg ttcctctccc tgacgggcca cgcggcggag atggaactcg aggaggaggc
     2941 cgaatcgccg gaacccgtga agaagcgcag gaagaggagg gagcgatgag cgccgtcgcg
     3001 gtccgtgccc ccgggatcgg cacgcaggcg gtgacgtccg tccaggattc gctcgtcgtc
     3061 gcgcgccgga acctgatgca gatcgtccgg atcccggcgg tgctcgtctt cgagctcgtc
     3121 cagccggtga tgttcgtcct gctcttcgtg tacgtcttcg ccggcaacat ccagaacgtg
     3181 ccgcccgaga tcgaggcgat cggcggctac gtgatgttcc ttatgccggg gatcatcatc
     3241 cagaacgcga tcttcggggc gacgaccacc gcgatcggga tcgccgagga tctgaaggcc
     3301 ggcttcgtcg accgcttccg ttcgctcccg atggcgcgct cggcgatgct cgccggccgg
     3361 gcgacgtcgg acctgatcaa gaacttcgtc ctgctcctcg tggtgatcgg gatcgggtac
     3421 ctggtgggat tctcgttcga gaacggcttc ctcggcgcga tcgcgatgat cgcgctgacg
     3481 atggcggtga gcttcacgtt ctcgtgggtg atggcctgga tcgccttgtc cgtgaagaag
     3541 ctggaggcgg tgcaggcggc cgcgttcacg ggcatcttcc cgctggtgtt catgagctcc
     3601 gcgatcgtgc cgatcgcggg gatggccccc tgggtccagg tggtcgcgcg gaacaacccg
     3661 atcacccact gggcgaacct cgcccgctac ttcgcgttcg gcgagtactc gacgatgggg
     3721 ggtacgcccg agcaattgat gctgtggtca gctctctgga tcgtcgggat cgtcgcggtg
     3781 ttcatcccgc tctcgatccg gctgtacaac aagctgacct agggaacaga cctcacgcat
     3841 ctcgcgttga ggcctgggtc gaccagagag gatcgaagcg tgccgttgct gggcttcaag
     3901 ggcgagaacg cgagagagaa ggtcatcgtc ctcctgacga tgtgcttcgc cctcgcgatg
     3961 gcgatgctcg acaacaccgt cgtcaacatc gccctcccga cgatcacccg caagctgaac
     4021 gcaaccgtct cggacctcca gtggatcatc gacggctacg tcctggcgtt cgcgtccctc
     4081 ctcctcaccg gcggcatcct cggcgaccgg tacggccgca agaagatgtt cctcaccggc
     4141 ttggcgatct tcacgctcgc ctccctgggc tgcggactct ccgcgtccgg aagtcagctg
     4201 atcgcgttcc gggccctcca aggcgtcggc gccgcgctgc tgatgccggg cacgctgtcg
     4261 atcctcacgg tcacgttccc gccggaagag cgctcgcgag cgatcggtat ctgggcgggt
     4321 gtgagtggtc tcgcgctcgc gctcggaccc acgctcggcg gctacatcgt cgagcactcc
     4381 ggatgggaga ccgtgttctt catcaacgtg ccgatcggcg
//
LOCUS       DASUVE010000202         2966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1039874, whole genome shotgun sequence.
ACCESSION   DASUVE010000202 DASUVE010000000
VERSION     DASUVE010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039874"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VF036_04355"
     CDS             complement(<1..210)
                     /locus_tag="VF036_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264494.1"
                     /translation="MRSALADVVWAPPPPAPDASDIGLIVFDRISQDVLDLVRGSSRG
                     GVRILAVGTTPEAVDDMGPWSLLDAG"
     gene            complement(450..947)
                     /locus_tag="VF036_04360"
     CDS             complement(450..947)
                     /locus_tag="VF036_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX6264495.1"
                     /translation="MDAAGVTVVVEIPGGSRNKYEMDHATGSIYLDRMLFTATRYPAD
                     YGFIEGTLGEDGDPLDALVLVGEPTFPGCRILARPVGVFGMKDEKGQDDKVLCVPLRD
                     PASSHLLDLDDLRPSLLAEIEHFFAVYKDLEDKKVETQGFQDLGAALVIIDEARQKAG
                     TTTHQ"
     gene            1028..1414
                     /locus_tag="VF036_04365"
     CDS             1028..1414
                     /locus_tag="VF036_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6264496.1"
                     /translation="MDVAEWVEAYRKAWETADTDLLLTLFTEDASYRSNPFEGPHVGH
                     DGIRAYWDGVTSQQRDARVSMGRPFVDGDRVAVEWWTKMVSEGEEVTLPGCLLLRFDA
                     EGRCSDLREYWNFAPGLREPFPGWGD"
     gene            complement(1411..2289)
                     /locus_tag="VF036_04370"
     CDS             complement(1411..2289)
                     /locus_tag="VF036_04370"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6264497.1"
                     /translation="MTPRVAVLGAGNMAGAMVGTLRRAGSDVTVWNRTRERAETVAET
                     HGARRASTTAEAVAGADVVLSSLADDEAVFATYLGETGAASAFGPEHVVLEMSTIAPD
                     TARRVGEAVAATGAAFLDAPVSGSVPTVEKGELTIMVGGDAAALERARPVLDALAAKV
                     FHVGALGAGATVKLAVNALVHAIDVGLSEALVLAEKAGVDRSAAYDVFAAGAAAAPFV
                     LYKRRAFEDPDNAQLTFTLDLMAKDLDLILSLAGTVGAPMEQAERNRELVERAIADGF
                     SGRDLSAIAEYFRRSG"
     gene            complement(2286..>2966)
                     /locus_tag="VF036_04375"
     CDS             complement(2286..>2966)
                     /locus_tag="VF036_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6264498.1"
                     /translation="LVVGGTHGLGRDVARHYAEAGRRVVVTGRDTERAALAAKELGGS
                     VDGLAFDLSQPDSIAPAVADLGSIDRLVLAALDRDLNTVKDYDIEAAIRLVTLKLVGY
                     TEMVHAVLPRLTPEASILLFGGLAKLRPYPGSTTITTINHGVDGLVRTMAAELAPIRV
                     NALHPGIVADSPFWAPKTEALEATKAKTPTGRLVVMRDVTDAAVFLLENPSMHGTELR
                     IDGGWSLT"
BASE COUNT          449 a         1002 c         1028 g          487 t
ORIGIN      
        1 tcccgcgtcg aggagcgacc agggacccat gtcgtcgacg gcctcggggg tcgtcccgac
       61 ggcgaggatg cggaccccgc ctcggctgga tccccgtacc agatcgagaa cgtcttggga
      121 gatcctgtcg aacacgatca atccgatgtc ggatgcatcg ggggccggtg gtggcggagc
      181 ccacaccacg tcggccaggg cggatcgcac gctggatgcc gttgctgagg cctcactggt
      241 cacgaagcgg atccagctcg tcatcggtcg cgaagcacgc cccctcggtg ggttgccgat
      301 gtacgaaggg tcgcgcagcg atcggcgacg gacaagtcgc cgcacctgcc taggcggata
      361 ggccgacgct ccgtggccgt tcgtcacgag gcgtccccct atcgggggat agtcatcggg
      421 tcaccgacct gagtgcttcc cgacactcct cactgatgcg tcgtcgttcc ggctttctgc
      481 cgcgcctcgt cgatgatcac gagcgcggct ccgagatcct ggaagccctg cgtctccacc
      541 ttcttgtctt ccaggtcctt gtagacggcg aagaagtgtt cgatctcggc gagcagggag
      601 gggcggaggt cgtcgaggtc caggagatga gacgacgccg ggtcgcgaag ggggacgcac
      661 aggaccttgt catcctgccc cttctcgtcc ttcatcccga agacccccac cggtcgcgcg
      721 aggatccggc atccagggaa cgtgggctct ccgacgagga cgagggcatc gagaggatcg
      781 ccatcctctc ccaacgtccc ctcgatgaat ccgtagtcgg ccgggtatcg ggtcgccgtg
      841 aagagcatcc ggtcgaggta gatggatccc gtcgcgtggt ccatctcgta cttgttccgg
      901 gatccacccg ggatctccac gacgaccgtc acgcctgctg cgtccatgtt cgaaccatag
      961 tcggctccct ctgatccggt cgcgaccggc gccggtcatg cgacccacca cccgataccc
     1021 tcgggcgatg gacgtcgcgg agtgggtgga ggcataccgg aaggcgtggg aaacggctga
     1081 cacggacctt ctcctcaccc tcttcaccga ggacgccagc taccgctcga acccgttcga
     1141 ggggccgcat gtcggacacg acgggatccg cgcgtactgg gacggtgtga cgagccagca
     1201 gcgcgacgcc cgcgtctcga tgggcaggcc gttcgtggac ggtgaccgcg tcgcggtgga
     1261 gtggtggacg aagatggtct ccgaaggtga agaggtgacg ctcccgggat gcctcctgct
     1321 ccggttcgat gctgaagggc gatgcagcga cctgcgggaa tactggaact tcgccccggg
     1381 actccgcgag ccgttccctg gttgggggga ctagccggac cggcggaagt actcggcgat
     1441 cgcgctgagg tcacggccgg agaatccgtc ggcgatcgcc cgctcgacga gttcgcggtt
     1501 gcgctcggcc tgttccatgg gagcgcccac cgtgccggcg agcgacagga tcaggtccag
     1561 gtccttggcc atgaggtcca gtgtgaacgt gagttgcgcg ttgtccggat cttcgaacgc
     1621 acggcgtttg tacagcacga acggcgctgc ggcagctccg gccgcgaaga cgtcgtaagc
     1681 ggcgctccgg tcgacgccgg ccttctccgc cagcaccaga gcttcggaca gacctacgtc
     1741 gatcgcgtgg acgagggcgt tgaccgcgag cttcaccgtg gcgccggcgc cgagcgcccc
     1801 gacatggaag accttggcgg cgagtgcgtc gagaacgggt ctggctcgct cgagcgccgc
     1861 ggcgtccccg ccgaccatga tcgtgagctc gcccttctca accgtgggca cgctaccgga
     1921 gacgggcgcg tcgaggaacg cggctccggt tgccgccacg gcttcgccga cgcgtcgggc
     1981 ggtatccggc gcgatcgtgc tcatctcgag cacgacatgc tcgggaccga acgcgctcgc
     2041 ggctcctgtc tcgccgaggt acgtcgcgaa caccgcctcg tcgtcggcga ggctgctgag
     2101 cacgacgtcg gcgccagcga cggcctccgc cgtggtcgat gccctccgcg ctccgtgggt
     2161 ctccgcaacg gtctcggccc gctcgcgcgt ccggttccat acggtgacgt cggatcccgc
     2221 ccgacggagc gttccgacca tcgctccggc catgttcccg gcgcctagca cggcgacgcg
     2281 gggcgtcatg tgagggacca gcctccgtcg atccggagct ccgttccgtg catcgagggg
     2341 ttctcgagga ggaacacggc cgcgtcggtc acgtcgcgca tgacgacgag gcggccggtc
     2401 ggcgtcttcg ccttcgtggc ctcgagggcc tcggtcttcg gtgcccagaa cggactgtcg
     2461 gccacgatcc ccgggtgcag ggcgttcacg cggatcggcg cgagttccgc ggccatcgtg
     2521 cggacgagcc cgtcgacgcc gtggttgatc gtcgtgatcg tcgtggagcc ggggtagggc
     2581 cggagcttcg cgaggcctcc gaagaggagg atcgaggcct cgggcgtgag ccgcgggagg
     2641 acggcgtgga ccatctcggt gtagccgacg agcttcagcg tcacgagccg gatcgcggcc
     2701 tcgatgtcgt agtccttcac ggtgttcagg tcacggtcga gtgccgcgag caccagccga
     2761 tctatcgatc cgaggtccgc cacggccggc gcgatcgagt ccggctgcga gagatcgaag
     2821 gcgagcccgt cgaccgagcc gcccagctcc ttcgccgcga gggcggcgcg ctcggtgtcc
     2881 cgccccgtca ccacgacgcg gcgtcccgcc tccgcgtagt gacgcgcgac atcccgcccg
     2941 agaccgtgcg tccccccgac gaccag
//
LOCUS       DASUVE010000203         4587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_850619, whole genome shotgun sequence.
ACCESSION   DASUVE010000203 DASUVE010000000
VERSION     DASUVE010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850619"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VF036_04380"
     CDS             complement(<1..301)
                     /locus_tag="VF036_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264499.1"
                     /translation="MASDADLWTCPRCGARFVTRNLWHGCGDYSVEGFFEGTPARLRE
                     LYDAWVSLVGEFGPFEQVPTKSRIAFMVRVRFAGVARLRTDGLVCSFWLKRRVDSP"
     gene            complement(311..1147)
                     /locus_tag="VF036_04385"
     CDS             complement(311..1147)
                     /locus_tag="VF036_04385"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX6264500.1"
                     /translation="MSLYRLEEQIPTLTAPVLLVAFDGWIDAAGAATACANHVADGGR
                     VVASFDVDALHDYRARRPVLDVVDGVLARMQWPEIVIRHVAFDGRDLLVLVGPEPDYK
                     WKQLGDDLLELALRLGVVEWISLGAIPAAVPHTRPVPVLATASTDASLRDGEQQGPGG
                     VLRVPAAALSAVEMTVAAAGIPAVGYYAQVPHYIGAAAYAPATVALLQHVERRLGVTI
                     PVRALGEEALSQRERLDAAIAEDADTREYLARLEAMASEERIPSGDDLATEIERYLKD
                     QS"
     gene            1245..2207
                     /locus_tag="VF036_04390"
     CDS             1245..2207
                     /locus_tag="VF036_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX6264501.1"
                     /translation="MRFFAVAAGAILFVAQAGCAGDVYGEFPSPPTPVFTATPSAEAD
                     NGILAEIPVDGSPCFLAEAGDRVWVTAFDGNELAEIDPETNEVVSTQRMPGGPCGLLD
                     VDGTLWIETPNAGAIVRFDPVRGEVIDRLRVPGGVFGLTSTPSGIWGVAGQGEEVVQI
                     DPASGEIVGRVGVDGPLGGLVFAREQVWVAAAGTLVRIDPRTQTVVDQIELESFEPEG
                     LAVDGDILWVSSSFEESVLRFDLRTMKVRDRVPVDDALFGGIVLGDSYWVSGNNTLYR
                     LDVETGEVEDELELVGFGPIPAAGDLWTVDLIANAVFRLDERAP"
     gene            2277..2708
                     /locus_tag="VF036_04395"
     CDS             2277..2708
                     /locus_tag="VF036_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264502.1"
                     /translation="MSAPDQNVYRVHLADPGGRISALDVPTEHFDSMERGISLGTTTI
                     VPWHRVIRYTREVVQAVDDAFLSRAEIRAWLDDGSDEGERIVVRADRFDAGAWTADFL
                     VERTLDIEGGVVHLTKIHVPWNRVLEYERLPVRVETPSRPD"
     gene            complement(2718..3107)
                     /locus_tag="VF036_04400"
     CDS             complement(2718..3107)
                     /locus_tag="VF036_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6264503.1"
                     /translation="MSEDRYERGRKRFLEVHDEKALAAVEGLGDLGRAIVEVAYGDIY
                     TRPGLALRDREIAAVAALVATGRSSQIPQHLRASLKAGLSPEELREIILQTATIAGFP
                     PAMNAMSTLKSVLREVGANEAGEASSE"
     gene            complement(3177..4376)
                     /locus_tag="VF036_04405"
     CDS             complement(3177..4376)
                     /locus_tag="VF036_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX6264504.1"
                     /translation="MRFAPTDVGFIADPYRDYDELRRSAPVVYDEATDHWLISRYADV
                     DALLRDRRFGRTYLHTSTHEEMGRPAPPDWHAPFWDLINAGILDMEPPDHTRVRRLVA
                     KAFTPRFVESLRPRVHAIFEDLLEGVAGAGEFDLLPTLAEPLPVIVIAEMLGVPETDH
                     HLLRPWSGDIVKMYELNPSQPAQHDAVRASLEFSGYLRELARERRTAPGDDLISGLVH
                     VIDEGDTLTEDELVGTCVLLLNAGHEATVNATLLGWWTLFRHPDRLRQLRADPSLLPT
                     AIEELLRFQTPLPMFERWVLEPFKLHGVEIPRGAELGLLFDSANRDPEVFEDADELRL
                     DRKPNPHLTFGAGIHFCLGAPLGRMELQISFGTMLERFPSLELVEEPTFRPNYIIRGL
                     DGLRVHA"
BASE COUNT          726 a         1579 c         1590 g          692 t
ORIGIN      
        1 ggggcgagtc gacccgccgc ttcagccaga acgagcacac gagcccgtcg gtccggagcc
       61 gcgcgacccc cgcgaaccga acccggacca tgaacgcgat ccgcgacttc gtcgggacct
      121 gctcgaacgg gccgaactcg cccaccagcg acacccaggc gtcatagagc tcgcggagac
      181 gagccggcgt gccctcgaag aacccctcga ccgagtagtc gccgcagccg tgccacaggt
      241 tgcgggtcac gaagcgggcg ccgcaccgcg gacaggtcca cagatcggcg tcgctcgcca
      301 cggccgcccg ctaggactga tccttcaggt accgctcgat ctccgtcgcg aggtcgtcgc
      361 ccgaggggat ccgctcctcg ctggccatcg cctccagtcg ggcgaggtac tccctggtgt
      421 cggcgtcctc cgcgatcgcc gcatccaggc gttcccgctg cgagagcgcc tcctcaccga
      481 gcgccctcac cgggatcgtg acgccgagac gccgctcgac atgctgcagg agggccaccg
      541 tggcgggcgc gtacgcggcc gccccgatgt agtgggggac ctgcgcgtag tagccgacgg
      601 cggggatccc ggccgccgcc accgtcatct ccaccgcgga caatgccgcc gcgggcaccc
      661 gcaggacgcc gcccggtccc tgttgctcac cgtcgcggag cgacgcgtcg gtggacgccg
      721 tcgccagcac gggaacgggg cgagtgtggg ggacggcagc cgggatcgca cccaggctga
      781 tccactcgac gaccccgagc cgcaacgcca gctcgagcag gtcgtccccg agctgcttcc
      841 acttgtagtc gggctccggg cccacgagga cgaggagatc tcggccgtcg aaggccacgt
      901 gccgtatcac gatctcgggc cactgcatcc tcgccagcac gccgtccacc acatccagga
      961 ccggccgcct cgctcgatag tcgtgcagcg catcgacgtc gaaggaggcg acgacccggc
     1021 ccccgtccgc cacgtggttc gcacacgcgg tggcggctcc cgccgcgtcg atccagccgt
     1081 cgaacgccac gagcaggacc ggcgctgtga gcgtggggat ctgctcctcg aggcgataga
     1141 ggctcaccct ccgaggatag ccccaggagc cggaagagcc gttccccgcc tcggtgacgt
     1201 atgagtgtga cgtggatggg gttcgaaccg gacgacgagg gtccttgcgg ttctttgccg
     1261 tcgcggcggg cgcgatcctg tttgtcgcgc aagcgggatg cgccggagac gtctacggcg
     1321 agttcccctc gccgcccacg cccgtgttca cggctacacc gagcgccgaa gccgacaatg
     1381 gcatcctcgc cgagatcccc gtggacggga gcccctgctt cctcgccgag gccggtgatc
     1441 gggtctgggt caccgcgttc gacgggaacg agctggccga gatcgatccg gagacgaacg
     1501 aggtggtctc gacgcaacgg atgccgggag gaccgtgcgg cttgctcgac gtggacggca
     1561 cgctgtggat cgagacgccg aacgcggggg cgatcgtgcg attcgacccc gtgcgtggcg
     1621 aggtgatcga ccgtctccga gttcccggag gggtgttcgg cctcacgtcg acgccgtcgg
     1681 gcatctgggg cgtcgccggc cagggcgagg aggtcgtcca gatcgatccc gcgtcgggcg
     1741 agatcgtcgg gagggtgggg gtggacggac cgctcggagg tctcgtgttc gctcgcgaac
     1801 aggtctgggt cgccgcggcc gggacgctcg tgcggatcga tccccgcacc cagaccgtcg
     1861 tcgaccagat cgagctcgag tccttcgagc cggagggact ggcggtggac ggcgacatcc
     1921 tctgggtctc cagctccttc gaggagagcg tgctgcggtt cgacctccgg acgatgaagg
     1981 tgcgagaccg cgtgccggtc gacgacgcgc tgttcggcgg catcgtcctc ggcgactcct
     2041 actgggtctc ggggaacaac accctctacc ggctcgacgt cgagaccggc gaggtcgagg
     2101 acgagctgga gctcgtcggc ttcgggccca tcccggccgc cggagacctg tggaccgtgg
     2161 atctcatcgc caacgcggtc ttccgcctcg acgaacgcgc gccgtgaggg cgagccattc
     2221 gaactagccc ttcgacggat gccgccttca tccggtcggg gatacgcttt cgagagatga
     2281 gcgcgcccga ccagaacgtc taccgcgtcc acctggccga tccggggggc cgtatcagcg
     2341 cgctcgacgt cccgaccgag cacttcgact cgatggagcg tgggatctcg ctcgggacga
     2401 ccacgatcgt cccgtggcat cgggtgatcc ggtacacccg ggaggtcgtc caggccgtcg
     2461 acgacgcctt cctcagccgt gcggagatcc gcgcctggct cgacgacggc tcggacgaag
     2521 gcgagcggat cgtggtgcgg gccgaccgtt tcgacgccgg cgcgtggacg gcggacttcc
     2581 tcgtggagcg aacgctcgac atcgagggcg gcgtcgtgca cctgacgaag atccacgtcc
     2641 cgtggaaccg ggtgctggaa tacgaacggc tcccggtacg ggtcgagacc ccgagccgac
     2701 cggactgatc cccgttttca ctcgctcgac gcttctccgg cctcgttcgc gccgacctct
     2761 ctcaggacgc tcttcagcgt ggacatcgcg ttcatcgccg gcgggaatcc cgcgatcgtc
     2821 gccgtctgca ggatgatctc gcgcagctcc tcgggtgaga ggcccgcctt gagggaggcc
     2881 cggaggtgtt gggggatctg ggaggagcgc ccggtcgcca cgagcgccgc gaccgccgcg
     2941 atctcgcggt cacgcaacgc gaggcccggc cgcgtgtaga tgtcgccgta cgcgacctcc
     3001 acgatcgccc gaccgaggtc gccgagacct tcgaccgcgg ccagcgcctt ctcgtcgtgc
     3061 acctcgagga atcgctttcg ccctcgctcg tacctgtcct cgctcacggc attcctctct
     3121 tcgacgccgg cgatcccgct ggcgccgatg atccccccca cgggcgatgc tcggcgttat
     3181 gcatggaccc ggaggccatc gaggcctcgg atgatgtagt tcggtcggaa ggtcggctcc
     3241 tccacgagct ccaggctcgg gaaacgctcc agcatcgtcc cgaacgagat ctgcagctcc
     3301 atcctgccta ggggagcgcc gagacagaag tggatccccg ctccgaacgt caggtgcggg
     3361 ttcggcttgc ggtccagccg cagctcatcg gcgtcctcga acacctcggg gtctcggttg
     3421 gcagagtcga acagcagccc gagctctgcc cctctcggga tctcgacccc gtggagcttg
     3481 aacggttcga gcacccagcg ctcgaacatc ggcaacggcg tctggaaccg cagcagctcc
     3541 tctatcgccg tcgggagcaa cgagggatcg gcccggagct gccgcagccg atccgggtgc
     3601 cggaacaggg tccaccagcc gaggagggtg gcgttcaccg tcgcctcgtg accggcgttc
     3661 aagagcagga cgcacgtacc gacgagctcg tcctcggtca gcgtatcgcc ctcgtcgatc
     3721 acgtgcacga gaccggagat caggtcatcg ccgggcgcgg tccggcgttc ccgcgccagc
     3781 tcccggaggt acccggagaa ttcgaggctc gcgcggacgg cgtcgtgctg ggccggctgg
     3841 gacgggttca gctcgtacat cttcacgatg tcgccggacc acggtcgaag gaggtggtgg
     3901 tcggtctcgg ggaccccgag catctccgcg atcacgatga ccggcagcgg ttccgccagc
     3961 gtgggcagga ggtcgaactc tcccgcgccg gccactcctt cgagcaggtc ctcgaagatc
     4021 gcatggacgc gggggcggag ggactcgacg aacctcggtg tgaacgcctt cgcgacgagc
     4081 ctgcgcacgc gtgtgtgatc gggaggctcc atgtcgagga tccccgcgtt gatcaggtcc
     4141 cagaacggcg cgtgccagtc gggcggtgcc ggccggccca tctcctcgtg ggtcgacgtg
     4201 tggagatacg tccggccgaa ccgccgatcc cgcagcaggg catcgacgtc cgcgtagcgc
     4261 gagatcagcc agtgatcggt cgcctcgtcg tagacgacgg gcgcggatcg acggagctcg
     4321 tcgtagtcgc ggtaggggtc cgcgatgaac cccacatcgg tcggcgcgaa ccgcatcgcc
     4381 cggacgatac ctgggatgag cggaggggaa ggctcggggg tgttccgatc gacgcagccg
     4441 ggtatcgatc cgatcagaac ccgctaccgg aggtgaggtc atggcgagca ggaagcaggt
     4501 caacgccgcg aagcgcaaca tcaagagcgc gcagaaggcg gcgcggaaga agcggacgat
     4561 ctcgaagctc ccgaagtccg tgcgaca
//
LOCUS       DASUVE010000204         4273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1040286, whole genome shotgun sequence.
ACCESSION   DASUVE010000204 DASUVE010000000
VERSION     DASUVE010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040286"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /gene="ftsZ"
                     /locus_tag="VF036_04410"
     CDS             complement(<1..1129)
                     /gene="ftsZ"
                     /locus_tag="VF036_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX6264505.1"
                     /translation="MDAPQNYLAVIKVVGIGGGGVNAVNRMIEAGLRGVEFTAVNTDA
                     QTLLMSDAEVKLDIGRETTRGLGAGSDPDVGRRAAEEHADEIEEIIKGADMVFITAGE
                     GGGTGTGGAPTVAEVARNLGALTIGVVTRPFGFEGRKRATQADLGITELKKAVDTLIV
                     VPNDRLLQVADANMPMIDAFRMADQVLYQGVDGITSLITTPGLINLDFADVKSIMAGA
                     GSALMGIGLGSGQDRATEAAKAAISSPLLESSIDGAKGVLLSIAGPTDLTLHEVNAAA
                     DAITRVAHPDANIIFGAVVDDALGEEVRVTVVAAGFDKVTAVSDSRFESRLSRLLEEP
                     GEQTEEEEPLPSILAEDDEDRVFTNGEQSPPVSFDAQEDLDI"
     gene            complement(1371..2054)
                     /locus_tag="VF036_04415"
     CDS             complement(1371..2054)
                     /locus_tag="VF036_04415"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX6264506.1"
                     /translation="MGLALVVAATIAVAATYTPLFAAGDIRLDAPDGMTRADVLAVAR
                     VTDRSNVFHLDAGAVERRLERDPRILRATVTTSLPDRIAIEVVPRTAVGLLGAGGELV
                     GADGVVIGPAESTPDLPELMTPDGRPVEANDLVAAAATAGALGPALRGQVEAVIVAPD
                     GTVRVRLAAGFPATFGDATELDAKAVSLAALVGWMREEGVTIVSADVTVPGSPTAQLE
                     RGSHAVPVP"
     gene            complement(2089..3027)
                     /gene="murB"
                     /locus_tag="VF036_04420"
     CDS             complement(2089..3027)
                     /gene="murB"
                     /locus_tag="VF036_04420"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX6264507.1"
                     /translation="MTVPDLAEAERILRARLGPGVRVGFPIAPLTTFRIGGPAALFVE
                     AQDDGALMAVREAVDAADVPLAVIGKGSNMLVSDDGFPGIVVRLGRGFRWAGRDGDRL
                     SAGGAMPLPAMAGVAMRHRLSGLEFGVAIPASLGGAVKMNAGAHGRSMSDVVDRVEVF
                     PLTGSGPRRSLASSEAGFAYRRSRLPDASIVVGATVRLRPGAEDEIRAHMEEARGWRR
                     RTQPLAEPNCGSVFKNPDGDHAARLVESAGAKGLRVGGARVSDKHANFIVADPGATAS
                     DVWDLIQQVRSRVERHAGIRLETEVELMGAVGDADD"
     gene            complement(3014..>4273)
                     /gene="murC"
                     /locus_tag="VF036_04425"
     CDS             complement(3014..>4273)
                     /gene="murC"
                     /locus_tag="VF036_04425"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX6264508.1"
                     /translation="DGLRELSALGARVRVGHDAASLGRPDAVVISSAIAEDNPELIAA
                     RATGLPVWARQQAIAAVAEAHRSIAVAGTTGKTTTTSMIATVLEREGLEPTYLIGGDL
                     NESGSGAKAGTGDLFLFEADESDGSFLLTDPAIGVVTNIELDHVDFYPGGAEELRMAF
                     AEFCARSGQVVVCGDDTGARDAVSLAGVAAIRYGTAADADVRLTIATTGPGGASGSLL
                     VAGVEVPLSLRIDGAHNLLDAAAAVTVSELVGVAPSDAARALASFAGVHRRFEERGRV
                     RGAAFFDDYAHVPTELSVTLGVARARRPGRLVAVFQPHRYSRTQALWRELGAALTEAD
                     LVVVTDVYGANQEPIPGVTGALVVDGVRAAAPDREVVYAPHREDAIAWLAREVRDGDL
                     VMTLGCGDIWMVADAALARIREADDRA"
BASE COUNT          622 a         1583 c         1452 g          616 t
ORIGIN      
        1 ggatgtccag atcctcctgc gcgtcgaaac tgaccggtgg ggactgctcg ccgttcgtga
       61 agacccggtc ctcgtcgtcc tcggccagga tcgacgggag cggttcctcc tcctcggtct
      121 gctcaccggg ctcctcgagc agccgcgaga ggcggctctc gaagcgacta tcggagacgg
      181 ccgtcacctt gtcgaagccc gcggccacga cggtgacgcg gacctcctcg ccgagggcgt
      241 catcgacgac cgctccgaag atgatgttgg cgtccgggtg ggccacccgc gtgatcgcgt
      301 cggccgccgc gttgacctcg tgcagggtca ggtcggtcgg accggcgatc gagaggagca
      361 cacccttcgc accgtcgatc gaggactcga ggagcggcga gctgatcgcc gccttcgccg
      421 cttccgtggc acggtcctgg cccgatccga ggccgatccc catcaacgcc gatcccgccc
      481 cggccatgat cgacttgacg tcggcgaagt cgaggttgat gagcccgggc gtcgtgatca
      541 gcgacgtgat gccgtcgaca ccctgataca ggacttggtc cgccatccgg aacgcgtcga
      601 tcatcggcat gttggcgtcg gcgacctgca gcagccgatc gttgggcacg acgatgagtg
      661 tgtcgacggc cttcttcagc tccgtgatcc cgaggtcggc ctgggtcgcg cgcttgcggc
      721 cctcgaaccc gaacggccgg gtcacgacgc cgatcgtgag ggcgcccagg ttccgcgcga
      781 cctccgcgac ggtcggcgcg ccaccggtcc cggtcccgcc gccctcgccg gcggtgatga
      841 agaccatgtc ggcacccttg atgatctcct cgatctcgtc ggcgtgctcc tccgcggcac
      901 gccgcccgac atcggggtcg gaccccgcac cgagcccccg cgtggtctcc cggccgatgt
      961 cgagcttcac ctcggcatcc gacatcagca gggtctgtgc gtccgtgttg acggcggtga
     1021 actccacgcc ccggaggcct gcctcgatca tccggttcac cgcgttcacg ccgcccccgc
     1081 cgatcccgac gaccttgatc acggcgaggt agttctgcgg agcgtccatc atccgaagcc
     1141 ctcccgggcc ccggcccgcg tactcgggat gctcgttctc gtgtgctggc tcggtgttgc
     1201 gtctgctgca gtggtgccgt gctcagctgc aaaccctaag gctccacttg aacgttatta
     1261 ccatatctag ctcatgtgga ggtagcgacc ctacttggaa atcgcaggtc cgtcaagaag
     1321 tgtggagggc cggcacggga cctcgtctgc gctttctcgt gacaatccga tcagggaaca
     1381 gggaccgcgt gcgagccgcg ctcgagctgt gcggtggggc tccccggaac cgtcacgtcc
     1441 gccgagacga tcgtgacacc ctcctcccgc atccatccca ccaaggccgc cagggagacc
     1501 gccttggcgt cgagctccgt cgcatcgccg aacgtcgcgg ggaaaccggc cgcgagccgg
     1561 acccgaacgg tgccgtcggg cgcgacgatc acggcttcga cctgcccgcg caatgcaggg
     1621 ccgagtgcgc cggccgtcgc cgcagccgcg acgagatcgt tcgcctcgac gggcctcccg
     1681 tcgggggtca tgagctccgg gaggtcgggc gtcgactcgg cggggccgat caccacgccg
     1741 tcggcgccca ccagctcccc acccgcaccg aggagaccca ccgcggtccg agggacgacc
     1801 tcgatcgcga tgcggtccgg cagcgacgtg gtcaccgtcg cgcggaggat cctcggatcg
     1861 cgctcgagcc gccgctcgac cgcaccggcg tccagatgga acacgttcga gcggtccgtc
     1921 acgcgggcga cggcgaggac gtcggctcgc gtcatgccgt cgggagcgtc gagccggatg
     1981 tctcccgcgg cgaacagcgg cgtgtacgtg gcggcgaccg cgatcgtggc agcgacgacg
     2041 agagcgagtc ccacgacggc ggatcgacgg gccgcgggtc catgcgggtc aatcgtccgc
     2101 atcgccgacc gcccccatca gttccacctc ggtttccaat cggatccccg cgtggcgctc
     2161 cacccgggag cgaacctgct ggatcagatc ccacacgtcc gacgccgtcg caccggggtc
     2221 cgcgacgatg aagttcgcgt gcttgtcgga gacgcgcgcc ccgccgaccc ggagcccttt
     2281 cgctcccgcg gactcgacca gccgcgcggc gtggtcgccg tccgggttct tgaagacgga
     2341 cccgcagttc ggctcggcga gcggctgcgt ccgtcggcgc catcccctgg cctcctccat
     2401 gtgcgcccgg atctcgtcct ccgctccggg ccggaggcgg acggtggcac ccacgacgat
     2461 cgaggcgtcg gggagccggg atcgccggta cgcgaagccc gcttcggagg aagcgaggga
     2521 tcgtcgagga cccgagccgg tgagcgggaa gacctccacc cggtcgacga cgtccgacat
     2581 cgagcgcccg tgcgctccgg cgttcatctt caccgcgccg ccgaggctcg cggggatcgc
     2641 gacgccgaat tcgaggcccg agagccgatg ccgcatcgcg acgccggcca tcgcgggcag
     2701 cggcatcgct cccccggcgc tcagccggtc cccgtcgcgg cccgcccatc ggaacccgcg
     2761 acccaggcgc accacgatgc ccgggaaccc atcatcggag acgagcatgt tggagccctt
     2821 cccgatcacg gcgagaggga cgtccgcggc gtcgaccgcc tcacgcaccg ccatgagcgc
     2881 cccgtcgtcc tgtgcctcga cgaagagggc cgcgggaccg ccgatccgga acgtggtcag
     2941 tggggcgatc gggaacccca cccgcacacc cgggcccagt cgcgcgcgga ggatccgctc
     3001 ggcctccgcg aggtcaggca cggtcatcgg cctcacggat cctcgcgagc gcggcgtcgg
     3061 ccaccatcca gatgtcaccg catccgaggg tcatcacgag atcaccgtcg cggacctctc
     3121 tggcgagcca tgcgatcgca tcctctcggt ggggggcgta caccacctcg cggtcggggg
     3181 ccgcggcgcg cacgccgtcg accacgagcg cccccgtcac cccggggatc ggttcctggt
     3241 tcgcgccgta gacgtcggtc acgaccacga ggtccgcctc cgtgagcgcc gcgccgagct
     3301 cgcgccagag cgcctgggtc cgtgagtagc ggtgcggctg gaacaccgcg acgaggcggc
     3361 ccggccgccg ggcgcgcgcc acccccagcg tcaccgacag ctccgtcggg acgtgggcat
     3421 agtcgtcgaa gaacgcggcc cctcggaccc tcccccgttc ctcgaagcgg cgatgcacgc
     3481 cggcgaagga tgcgagcgcc cgagcggcgt cggagggagc gacccccacg agctccgaga
     3541 ccgtcacggc ggccgccgcg tccaggaggt tgtgggcccc gtcgatccgc agggacagcg
     3601 gcacctcgac acccgccacg aggagggagc cgctcgctcc cccagggccc gtcgtcgcga
     3661 tcgtcagccg cacgtccgca tccgcggcgg tgccgtagcg gatcgcggcg acgccggcga
     3721 gcgagacggc gtcgcgggca cccgtgtcgt ccccacagac gacgacctgt ccggagcgag
     3781 cgcagaattc tgcgaatgcc atgcgcagct cctccgcacc gccgggatag aagtccacgt
     3841 ggtcgagctc gatgttcgtg acgacaccga tcgcggggtc ggtcaggagg aaggagccgt
     3901 cgctctcgtc ggcctcgaag aggaacagat cgccggtgcc ggccttcgcg ccgctcccgc
     3961 tctcgttcag gtcgccgccg atcaggtacg tgggctccag gccttcccgc tcgagcacgg
     4021 tggcgatcat cgacgtcgtc gtcgtcttgc cggtcgtgcc cgcgaccgcg atcgatcgat
     4081 gcgcctcggc gacggcggcg atcgcctgct gacgggccca caccgggaga ccggtcgcgc
     4141 gagccgcgat gagctccgga ttgtcctcgg cgatcgcgct cgagatcacc acggcatccg
     4201 gacgtccgag gctcgcggcg tcatgcccca cgcggacgcg ggcgcccagg gccgagagct
     4261 ctcgcagccc gtc
//
LOCUS       DASUVE010000205         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1229035, whole genome shotgun sequence.
ACCESSION   DASUVE010000205 DASUVE010000000
VERSION     DASUVE010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229035"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="VF036_04430"
     CDS             complement(<1..118)
                     /locus_tag="VF036_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6264509.1"
                     /translation="MTTDVLDRTFAALADPTRRAILSRLAEGEASVTELAAPF"
     gene            complement(128..616)
                     /locus_tag="VF036_04435"
     CDS             complement(128..616)
                     /locus_tag="VF036_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6264510.1"
                     /translation="MAQQANRLEVHTPGEDRIVMTRTFDAPRDLVFEAHTSCEHMSRW
                     WGPRRYEVTECDIDFREGGAWRIVHSGPEGERHVFFGEYREIVRPETISWTFGFEGMP
                     GDPGVETVTFEEHDGVTTLTAIAFFDTIEERDAVLGSGMLEGATETWERLEEYLGTMA
                     AA"
     gene            839..1066
                     /locus_tag="VF036_04440"
     CDS             839..1066
                     /locus_tag="VF036_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264511.1"
                     /translation="MRFSVRRGSGPDTAPAAPVPFEPTLDLARFERLIETAVRLRDAG
                     IAYEDVERRAMADEVSWNRALEEIDRERSDA"
     gene            1059..1469
                     /locus_tag="VF036_04445"
     CDS             1059..1469
                     /locus_tag="VF036_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX6264512.1"
                     /translation="MRRPRRREGGAAAVEFALIAPVLFIVLMGILEFGVAFLQVQSIR
                     TGVREGGRAAAVGAPIATVRQKTVDASSGSIPGDQTGNVSVSRQCSPQQIGEDVVVAY
                     DTSQLPGGGIMVRIPFIPTIEMRPVLSATFRCEA"
     gene            1472..>2365
                     /locus_tag="VF036_04450"
     CDS             1472..>2365
                     /locus_tag="VF036_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX6264513.1"
                     /translation="MREREEGAVVVIVAICMVALMAAVAITVDVGGLYLRRRELVNGS
                     DSGALSAARTCARGGADVRFATPELASDTAVRANGAITATEVAGTDIVEITQCGTQWG
                     HVTVRYTSQQALHFAPVLGFEYEKPVTTQATASWGLGSNNPVPIVLSSLVTVNCPIPP
                     SGWPAPGETCAFWYDNDRLNGGNFAFLSLNPHGWDVPIDSNCSQSQAGGTSTLTGWID
                     GSIPASVSLNWTQPTYVCTDSGIRGVGGKNGPNSQLWGALHDLIGQTRDFPINWEGPG
                     EPITGAPSQGMVFQQGNIDKYD"
BASE COUNT          353 a          850 c          810 g          352 t
ORIGIN      
        1 cgaacggcgc cgcgagctcg gtgaccgagg cctcgccctc ggccaggcgg gacaggatcg
       61 cccgcctggt cgggtcggcg agggccgcga aggtccggtc gagcacgtcg gtcgtcatct
      121 gcgcctccta ggcggccgcc atcgtcccga ggtactcctc gagccgctcc caggtctcgg
      181 tcgcgccctc gagcatgccc gagccgagga ccgcgtcgcg ctcctcgatc gtgtcgaaga
      241 aggcgatcgc cgtgagggtg gtcacgccgt cgtgctcctc gaaggtgacg gtctccacgc
      301 ccgggtcacc cggcatcccc tcgaacccga acgtccacga gatcgtctcg ggacgcacga
      361 tctcgcggta ctcgccgaag aacacgtggc gctcgccctc ggggcccgag tgcacgatcc
      421 gccatgcccc gccctcgcgg aagtcgatgt cgcactccgt cacctcgtac cgacgcggcc
      481 cccaccacct cgacatgtgc tcgcacgagg tgtgcgcctc gaacacgagg tcgcgcgggg
      541 cgtcgaacgt gcgcgtcatc acgatccggt cctcgcccgg ggtgtgcacc tcgagccggt
      601 tcgcctgctg tgccatgcca ccgctcctct ctcccgtgga ccgattaacc gtccggttat
      661 ctaacgtatc ggtaaactac gctcactgac ccgccgctgt caagcgccac ccggagccgc
      721 tgcggcacgg acttcgttca cggatccttc acggtgcatt aggcctttcg cccgaagcta
      781 ggtccgccgc gtagtgcttg gatcctcccc tgaacgaccg acgaggagaa ctccccccat
      841 gcgcttctcc gtccgcaggg gctccggccc cgacaccgcg ccggcggccc cggtgccctt
      901 cgaacccacg ttggacctcg cgcgcttcga gcgcctgatc gagacggcgg tgcgcctgcg
      961 cgacgcgggc atcgcctacg aggacgtcga acgccgtgcc atggccgacg aggtcagctg
     1021 gaaccgcgcc ctcgaggaga tcgaccggga gcggagcgat gcgtaggccg cgccgccgcg
     1081 agggcggggc cgcggcggtg gagttcgcgc tgatcgcgcc ggtgctgttc atcgtcctga
     1141 tgggcatcct cgagttcggg gtcgcgttcc tgcaggtcca gtcgatccgc accggcgtgc
     1201 gcgagggcgg ccgggcggcc gcggtcggcg ccccgatcgc gacggtgcgg cagaagacgg
     1261 tcgacgcgtc gtcgggctcg atcccgggcg accagaccgg caacgtgtcg gtgagccggc
     1321 agtgcagccc tcagcagatc ggcgaggacg tcgtggtcgc ctacgacacc tcccagctcc
     1381 ccggcggcgg gatcatggtg cgcatcccct tcatccccac gatcgagatg cgcccggtcc
     1441 tgtcggccac gttccgatgc gaggcgtgag gatgcgggag cgcgaggaag gcgccgtcgt
     1501 cgtcatcgtc gcgatctgca tggtcgccct gatggccgcg gtcgcgatca ccgtggatgt
     1561 cggcgggctc tacctgcgcc ggcgcgagct cgtgaacggg tcggactcgg gcgcgctctc
     1621 ggcggcccgg acctgcgcgc gcggcggcgc cgacgtccgg ttcgcgaccc cggagctggc
     1681 ctcggacacg gcggtccggg ccaacggcgc gatcaccgcg accgaggtcg ccggcaccga
     1741 catcgtcgag atcacgcagt gcggcacgca gtggggccac gtgacggtcc gctacacgag
     1801 ccagcaggcg ctgcacttcg cgccggtcct ggggttcgag tacgagaagc cggtcacgac
     1861 gcaggcgacc gccagctggg gtctcgggag caacaaccct gtgccgatcg tgctgagcag
     1921 cctggtcacg gtgaactgtc ccatcccccc gagcggatgg cccgcccccg gtgagacgtg
     1981 cgccttctgg tacgacaacg accggctgaa cggcggcaac ttcgccttcc tcagcctgaa
     2041 cccgcacgga tgggacgtgc ccatcgattc gaactgcagc cagtcgcagg ccggcgggac
     2101 gtcgaccctc acgggatgga tcgacggatc gatccccgcg agcgtgtcgc tgaactggac
     2161 ccagccgacg tacgtctgca ccgactcggg catccgcggt gtcggtggga agaacgggcc
     2221 gaactcgcag ctctggggcg cgctccacga cctgatcggg cagacacgcg acttcccgat
     2281 caactgggag ggaccggggg agccgatcac cggtgcgccg tcgcagggga tggtcttcca
     2341 gcaggggaac atcgacaaat acgac
//
LOCUS       DASUVE010000206         5789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_477204, whole genome shotgun sequence.
ACCESSION   DASUVE010000206 DASUVE010000000
VERSION     DASUVE010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477204"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VF036_04455"
     CDS             <1..206
                     /locus_tag="VF036_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6264514.1"
                     /translation="GHTVNYAARVASSARGGEIVVSSLVHDLLVQTGEFAFADARRVE
                     LKGIDGTEVVYPLAVASSEPSAD"
     gene            complement(270..488)
                     /locus_tag="VF036_04460"
     CDS             complement(270..488)
                     /locus_tag="VF036_04460"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6264515.1"
                     /translation="MDHGFAPGDPTRDAIMRVFRDASAAWTLDELARRFGISIGDAIR
                     VMSDLEAIDFVRRIDDEYVRGFGAANAS"
     gene            678..1757
                     /locus_tag="VF036_04465"
     CDS             678..1757
                     /locus_tag="VF036_04465"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6264516.1"
                     /translation="MEEILVYGASWCPDCRRAKKFLADQRVPYSWHDIEGDPGGVRVV
                     RDRNEGKNIIPTIVFPDGSHLSEPSNEELAEKLGLQRQAMQHVYDLIVVGGGPTGLTT
                     SIYAARENLETLIIDSKGLGGQAGVTERLDNYPGFPEGIGGGELADRFVKQAERYGVE
                     MLQAISVTSISADSGMTADGDELDVETANGDHYHARAVLLATGSSYRRTGADGEDDLI
                     GAGVHFCATCDGPFYKGSDELMVIGGGNSSLEEGIFLTQVAERVTIVERGDRLRGSRI
                     LQAKVLDHPRIKVELQHGVKEFRKEDGSGKLGSVVVEDLVSGTLEEQHPAGVFVFIGL
                     DPTPASCKGASTWTSAGSSRPIRRS"
     gene            1754..1897
                     /locus_tag="VF036_04470"
     CDS             1754..1897
                     /locus_tag="VF036_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264517.1"
                     /translation="MSSMPGVFAAGDVRDGSTKQLASAVGEGAAAAIQIRGYLDRLDR
                     RSM"
     gene            1912..2109
                     /locus_tag="VF036_04475"
     CDS             1912..2109
                     /locus_tag="VF036_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264518.1"
                     /translation="MSMQPNEIFELIVKADEKLKYATADKTGTRTQQARDLLTQARDA
                     ARAIGNEQLVQQAELRLSDLA"
     gene            complement(2106..2348)
                     /locus_tag="VF036_04480"
     CDS             complement(2106..2348)
                     /locus_tag="VF036_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX6264519.1"
                     /translation="MGRDLTCKELVELVTGYLDGSLPGRRRRRFEGHLAACDGCTRFL
                     RQTEATIRVTGTLTAEQVTEEQRAALLTAFRGWSTS"
     gene            complement(2348..2995)
                     /locus_tag="VF036_04485"
     CDS             complement(2348..2995)
                     /locus_tag="VF036_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6264520.1"
                     /translation="MTTPLPPGDDLALVARLRAGDESAFMTLVEHLQPSMLRVARMYV
                     STAAVAEEVVQETWLGVLRGLDRFEGRSSLRTWIFRILTNVAKTRGSREGRSVPFASL
                     TGDDLDGPAVDPDRFDTRETSSRGAWSTLPDDWTGIPEDRLLGRETLAVIGRAIDGLP
                     PMQAEVIRLRDVLGWTSEEVRNALDLTETNQRVLLHRARAKVRSALEAHLSSETT"
     gene            complement(3037..4104)
                     /locus_tag="VF036_04490"
     CDS             complement(3037..4104)
                     /locus_tag="VF036_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX6264521.1"
                     /translation="MTGTRTRITRLLVAVVFAVPVLLSGLSIASLAAPSAEEVEAAKA
                     KLAGLQHEFEVLAEQYNDAKYRLALIERRLAEAREQRDIAEQQAQRAESRLATRAVQA
                     YVGTGSQVDGLLGAESIAEFSDRLEFMGAVAEGDAEIARTASNARQEADWASDRFAAT
                     LSERQRQVATIENQLDRLDSMIAEQASLYEQLNQDRNDFLAAQRAALEQARQEAAQDA
                     PSADTTGDTGGYVPPVNGSAAEIAVGAAKSVIGTQYVWGSADPSVGFDCSGLTSWAWG
                     QAGVYIPHSSASQYSSLPQVPLGSVQPGDLIFYYSPISHVALYIGGGQIVHATHPGAD
                     GAVHIDSMYGYDPPVGAARPG"
     gene            complement(4254..4327)
                     /locus_tag="VF036_04495"
     tRNA            complement(4254..4327)
                     /locus_tag="VF036_04495"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4291..4293),aa:Pro,seq:cgg)
     gene            5234..5695
                     /locus_tag="VF036_04500"
     CDS             5234..5695
                     /locus_tag="VF036_04500"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX6264522.1"
                     /translation="MSDPTLKERLAAEITGALRAGEKVRLGALRMLSAAVTNREKELR
                     HDLSDDEFRDVAAKEMKKRAESIEAFEGAGREALAEKERAEREAIASYAPEPMSDGDL
                     DALVDEAFAATGATSEKEFGAVMGFLMGKAKGRVDGKVVQEKVRARLGGTP"
BASE COUNT          927 a         1932 c         2044 g          886 t
ORIGIN      
        1 tcggtcatac ggtcaactac gcggcccggg tcgcgtcatc ggcgcgagga ggcgagatcg
       61 tggtctcgtc actcgttcac gacttgctcg ttcagaccgg ggagttcgca ttcgccgatg
      121 ctcgccgcgt ggagctgaag gggatcgacg gcacggaggt cgtgtatccg ctcgccgtcg
      181 cctcgtctga gccctccgca gactgagccc agcccaagcc aactgagccc atccggccga
      241 catcaggcgc tcgcgacggc cgcgaccgcc taagaggcgt tcgcggcccc gaacccgcgc
      301 acgtattcgt cgtcgatccg gcgcacgaag tcgatcgcct cgaggtccga catcactcgg
      361 atcgcgtcgc cgatcgatat gccgaaacgt cgtgccaact cgtcgagcgt ccaggccgcg
      421 gaggcgtccc ggaagactcg catgatcgcg tcgcgcgtgg ggtcgccggg tgcgaagcca
      481 tggtccatgg tcgtcctcct cggtcgcagt gtctctcgga cgctcctgga gggccaaggg
      541 ttccttggaa ccttccggac gcgttccccg atcgttcacc ggtcccgtgg gccggatcct
      601 ccgtgacatc ggcgtaacgg acacaggcgg gcgcgatacg gtccgagcgg cggtcgccga
      661 ccgacgggga gtgagggatg gaggagatcc tcgtgtacgg cgcgagctgg tgccccgatt
      721 gccgccgcgc caagaagttc ctggccgacc agcgggtgcc gtattcctgg catgacatcg
      781 agggggaccc gggcggtgtc cgggtcgtcc gggatcgcaa cgagggcaag aacatcatcc
      841 cgacgatcgt gttcccggac ggttcgcatc tgtcggagcc ctcgaacgag gagcttgcgg
      901 agaagctcgg tctccagcgg caggcgatgc agcacgtgta cgacctgatc gtcgtgggcg
      961 gcggccccac cggtctgacc acatcgatct acgccgctcg cgagaacctc gagacgctga
     1021 tcatcgactc caagggtctc ggagggcagg cgggcgtgac ggagcggctc gacaactacc
     1081 cggggttccc ggaggggatc ggcggcggcg agctcgccga ccggttcgtg aagcaagccg
     1141 agcgctacgg tgtggagatg ctgcaggcga tctccgtcac gagcatctcc gccgactcgg
     1201 ggatgaccgc cgatggggac gagctcgacg tggagacggc gaacggcgac cactaccacg
     1261 cacgcgcggt cctcctcgcc accggatcct cctaccggcg gacgggcgcc gacggggagg
     1321 acgatctgat cggtgcgggc gtccacttct gcgcgacgtg cgacgggccg ttctacaagg
     1381 gctccgacga gctgatggtg atcggcggcg gcaattcgag tctcgaggaa gggatcttcc
     1441 tgacccaggt cgccgagcgg gtcacgatcg tggagcgagg cgaccggttg cgcgggtcca
     1501 ggatcctgca ggcgaaggtc ctcgatcacc cgcgcataaa ggtcgagctg cagcatggcg
     1561 tgaaggagtt tcggaaggag gacggttccg ggaagctcgg gtcggtcgtg gtcgaggatc
     1621 tcgtctcggg cacgctggag gagcagcacc ccgccggcgt gttcgtattc atcgggctcg
     1681 atccgacacc ggcttcgtgc aagggagcat cgacctggac gagcgcgggt tcgtcgcgac
     1741 cgatccgacg ttcatgagct cgatgccggg tgtgttcgcc gcgggcgacg tgcgggacgg
     1801 ctccacgaaa cagctcgcct cggcggtggg tgagggcgcg gcggcggcga tccagatccg
     1861 cggctacctc gaccgtctcg atcgccgctc gatgtgaggg atgatgtcga gatgtccatg
     1921 cagccgaacg agatcttcga actgatcgtc aaggcggacg agaagctcaa gtacgccacc
     1981 gcggacaaga cgggaacgag gacgcagcag gcgagggatc tgctcacgca ggcccgtgac
     2041 gccgcgcgtg cgatcgggaa cgagcagctg gtgcagcagg cggagttgcg actctcggat
     2101 ctggcttagc tggtcgacca gccgcggaat gcagtcagga gggctgccct ctgttcctcg
     2161 gtcacctgct ccgcggtgag ggtaccggtc acgcggatcg ttgcttccgt ctggcggagg
     2221 aatcgtgtgc acccgtcgca cgcggccagg tgcccctcga accgccgccg ccgccttccc
     2281 ggcaggctgc cgtcgaggta gccggtcacc agctccacca gctccttgca ggtcaggtcg
     2341 cgtcccatca cgtcgtctca ctcgagaggt gggcctccag cgcgctccgc accttcgcgc
     2401 gcgcgcgatg gaggagcacc cgctgattgg tctcggtcag gtcgagcgcg ttacggacct
     2461 cctccgacgt ccaacccagg acgtctcgca gccggatcac ctcggcctgc atcggaggga
     2521 gcccgtcgat cgccctgccg atgacggcga gggtctcgcg tccgaggagc cgatcctccg
     2581 ggatcccggt ccagtcgtcg ggcagcgtcg accaggcccc tcgcgacgag gtctcgcggg
     2641 tgtcgaagcg atcgggatcc acggccggcc cgtccaggtc gtcgccggtg agcgaggcga
     2701 acggcacgct ccggccctcg cgcgagccgc gggtcttcgc gacgttcgta aggatccgga
     2761 agatccaggt acggagtgac gaccgcccct cgaagcggtc gaggccgcga aggacgccga
     2821 gccacgtctc ctggaccacc tcctcggcga cggccgccgt cgagacgtac atccgagcga
     2881 cgcggagcat cgacggttgc aggtgctcga ccaaggtcat gaacgccgac tcgtcgccgg
     2941 cgcggagccg tgccacgagt gcgagatcgt ccccgggagg gagcggtgtg gtcacggacg
     3001 cggagcctac cgaggagccg cagtggaacc cgaggcttag cccggacggg ctgcgccgac
     3061 cggcgggtcg tacccgtaca tcgagtcgat gtggaccgcg ccgtccgccc cggggtgagt
     3121 ggcgtgcacg atctggccgc cgccgatgta gagggcaacg tggctgatcg gggagtagta
     3181 gaagatcagg tcgccgggct ggacgctccc gagcggcact tgggggagcg acgagtactg
     3241 cgaggccgac gagtgcggga tgtagacgcc cgcctgcccc cacgcccacg aggtgagccc
     3301 ggagcagtcg aagcccacgc tcgggtctgc cgatccccag acgtactggg taccgatcac
     3361 gctcttcgcg gctcccaccg cgatctcggc ggccgagccg ttgacgggcg gaacgtatcc
     3421 gccggtgtcg cccgtggtgt cggccgacgg tgcgtcctgt gcagcctcct gccgagcctg
     3481 ctcgagcgca gcacgctgcg ctgcgaggaa gtcgttgcga tcctggttga gctgctcgta
     3541 gagggatgcc tgctccgcga tcatcgagtc gagccgatcg agctggttct cgatcgtggc
     3601 gacctgtcgc tgtcgctcgc tgagggtcgc ggcgaaccga tcggacgccc aatcggcttc
     3661 ctgccgagca ttcgacgccg tgcgggcgat ctccgcgtcg ccttcggcga cggcacccat
     3721 gaactccaac cggtccgaga actctgcgat gctctcggcg ccgaggagcc cgtcgacctg
     3781 ggaaccggtc cccacgtagg cttgcaccgc tcgcgtcgcc aggcgggact ccgcacgctg
     3841 cgcctgctgc tccgcgatgt cgcgctgctc gcgcgcctcc gcgagcctgc gctcgatcag
     3901 cgcgagacgg tacttcgcgt cgttgtactg ctcggccagg acctcgaact cgtgctgcag
     3961 acctgcgagc ttcgctttcg ctgcctcgac ctcttcggcg gacggcgccg cgagggaggc
     4021 gatgctgagc ccggagagga gaacagggac cgcgaagacc acggccacga ggagccgtgt
     4081 gatccgcgtt cgcgtcccgg tcatttgctc ctaggtccga gtagtacgaa agctccgtcg
     4141 ccggagccct catccgaggc taccggtgcc cccaggggtc gtcaaacccc tgttgcgcga
     4201 tagcgaacat gcgtggggcg gaccgtttcc ggcccgcccc cgtgtatcgg ctgtcgggac
     4261 ggcgggactt gaacccgcga cctcagcgtc ccgaacgctg cgcgctacca agctgcgcca
     4321 cgtcccgagc ctctgattct agcggttcgt caccgggtgc cgacgctcct ggatgaaggt
     4381 ccaggtacgt ggcctccggc cggcaccaga accggaacgg cgcgtacttg ctcgttccga
     4441 gtccccggga cgtgtggagc accgcggacc ccatccggac cagccccgac gcgaacgcgc
     4501 gcggcatcga acagttggtc accagcgcgc cgatccaggg gagtcggacc tggccgccgt
     4561 gggtgtgccc ggccacgatc aagccgtatc cgagcgcggc cgcctccgga gccgtgtcgg
     4621 gagagtgcat cacggcgagg ccgaagtgat cgtcctcgct ccgcggagcc acacgtaggt
     4681 cgtggcgccc gatgtgggcg tcgtcgagac cgaggacctc ggtctcgagc ccgaggagcg
     4741 agatcgtgcg tcgcgagtcc gtgaggtctt cccacccgtc agcgacgagg gctcggatca
     4801 gatcgggcgc gcggccggga gccgcgcgcc gacgaggccg cccgggacgg aagtaggcca
     4861 ggtagttgag cggtcggggc acgaagtagt cgttcgatcc gaggacgaac cacgacgcga
     4921 tgcgaccgcg caccgcccgc acgctctcga ccgtcgtgcc gatcgcttcc ggctcgccca
     4981 ggaggtcccc cgtcacgacc gtgacgtccg ccgatggcag ccctgccaag aagcgcgtct
     5041 tccgagggtc gtgacgaacg aagtggagat cggacagatg gagcacgacg agtggctgcg
     5101 cggccgccgg taggatgtcc aggcggtgcc ggatcacccg gtatcggaac cgttcgatga
     5161 agatcccgta gagcacgcag ccgacgccga gcgcggcgac cgtcacgacc acggggaaca
     5221 cggggggagt gtaatgagcg acccgacgct gaaggagcgt ctcgccgcgg agatcaccgg
     5281 tgctctccgc gcgggcgaga aggtccgtct cggcgcgctc cgcatgctct cggcggccgt
     5341 caccaaccgg gagaaagagc tccggcacga cctctccgac gacgagttcc gcgacgtcgc
     5401 cgcgaaggag atgaagaagc gagccgaatc gatcgaggcg ttcgagggcg ccgggcgaga
     5461 ggcgctcgcc gagaaggagc gagccgagcg ggaggcgatc gcgtcctacg cgccggagcc
     5521 gatgtccgac ggcgatctgg atgcgttggt agacgaggcg ttcgcggcca ccggagccac
     5581 gtcggagaag gagttcggcg ccgtgatggg cttcctcatg ggcaaggcga agggccgcgt
     5641 ggacgggaag gtcgtgcagg agaaggtgcg cgccaggctg ggcgggacgc cctagcctcc
     5701 cccgccgttc cctccaccgt tcccgttccc tccaccgttc ccgttccctc caccgttccc
     5761 gttccctccg ccgttgccct cgtcgccca
//
LOCUS       DASUVE010000207         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_287821, whole genome shotgun sequence.
ACCESSION   DASUVE010000207 DASUVE010000000
VERSION     DASUVE010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287821"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VF036_04505"
     CDS             <1..517
                     /locus_tag="VF036_04505"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6264523.1"
                     /translation="RPLGYVTQTSAVSSAPPQVVYETISDLRNHLDWSGERASSQTFK
                     LLSIEAPDGPAAAGTAFTSSGAADNGTFHDRSEVTVATAPSAFVIETDAHLDRKRGKP
                     WDAHFIHRYDVAPEGEGSRITYTETIERVNYVPYWLQPGVRSIFKVYVNRADRKQLGN
                     LARLAEERAAR"
     gene            complement(519..1037)
                     /locus_tag="VF036_04510"
     CDS             complement(519..1037)
                     /locus_tag="VF036_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264524.1"
                     /translation="MPHSVGDLAEVLDNLGVFSMHPEEVLALCPSGFRRVPITSGDEF
                     YLSLQGFTAYQPAYAHTHPDSEEWVVVLEGSGKALLADNPVPLEGSLVIGRATAHPHG
                     FVSGDGPMTLLSIQLPRPSEPATTWDQPGETTDPIDCHSGGTCRRCYRCGGHSHNHRA
                     SVFVCENCTLEF"
     gene            1110..1529
                     /locus_tag="VF036_04515"
     CDS             1110..1529
                     /locus_tag="VF036_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6264525.1"
                     /translation="MTERTIIVDHVLFVVEDFPASRRLYTAALAPLGMDELYLEPNCV
                     SYGREDLDDFSICEGSPVTTAAHVAFDAADRSQVDAFFEAALAAGATTRGEPGVWIQY
                     SNRYYGAFVNDLHGNNVEAVFHSPVPITDAPERGGAP"
     gene            1682..2023
                     /locus_tag="VF036_04520"
     CDS             1682..2023
                     /locus_tag="VF036_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX6264526.1"
                     /translation="MRTDLWNYPEGTTTPLDIQGFKVRATDGEIGKIDEATYDVGSAF
                     IVVDTGPWILGRKVLIPAGMAERIDLDRREVEVRLTKDQIKNAPELDEVTDYRSDTYR
                     EQVGTYYSTYS"
BASE COUNT          397 a          751 c          764 g          346 t
ORIGIN      
        1 tcgtccactc ggctacgtga cgcagacgtc ggcggtgtcg tccgcgccgc cgcaggtcgt
       61 ctacgagacg atctcggacc tccggaacca tctcgattgg tccggcgagc gcgccagcag
      121 ccagacgttc aagctcctgt cgatcgaggc gcccgacggg ccggcagccg cggggaccgc
      181 cttcacctcg tcgggcgcgg ccgacaacgg cacgttccac gatagatccg aggtgacggt
      241 cgcgaccgct ccgagcgcgt tcgtgatcga gaccgatgcc cacctcgatc ggaagcgtgg
      301 gaagccctgg gacgcgcact tcatccatcg ctacgacgtg gcgcccgagg gcgagggctc
      361 ccggatcacg tacacggaga cgatcgaacg cgtgaactac gttccctact ggctccagcc
      421 gggtgtgcga tccatcttca aggtctacgt gaaccgtgcg gaccgcaagc agttggggaa
      481 cctggcgcgg ctcgccgagg aacgggcggc ccggtagctc agaactccag ggtgcagttc
      541 tcgcagacga agacgctcgc ccgatggttg tggctgtggc cgccgcaccg atagcagcgc
      601 cggcacgtgc cccccgagtg gcaatcgatc gggtcggtcg tctcccccgg ttggtcccac
      661 gtggtcgcgg gctccgacgg gcgggggagt tggatcgaca ggagcgtcat cgggccgtcc
      721 ccgctgacga agccgtgggg gtgcgcggtc gcccgaccga tcacgaggct cccctcgagc
      781 gggaccgggt tgtcggcgag cagcgccttc cccgagccct cgagcaccac gacccactcc
      841 tccgagtccg ggtgggtgtg cgcataggcc ggctggtacg cggtgaaccc ctggagggag
      901 aggtagaact cgtcgcccga ggtgatcggg acccgccgga acccggaggg acagagcgcg
      961 aggacctcct cgggatgcat cgagaagacg ccgaggttgt ccaggacctc cgcgaggtcg
     1021 ccgaccgagt ggggcatggg tagcatcctc tcgcatcgga ggccgccggc caaccggagg
     1081 cgagtcgacg gccgaggaag gaggtttgca tgaccgagcg aacgatcatc gtggaccacg
     1141 tcctgttcgt cgtcgaagac ttcccggcga gccggcgcct ctacacggcg gcgctcgccc
     1201 cactggggat ggatgagctg tacctggagc ccaactgcgt ctcctacggc cgggaggacc
     1261 tcgacgactt ctcgatctgc gaaggctctc cggtcacgac cgcggcgcac gtggcgttcg
     1321 atgcagcgga tcgctcgcag gtggacgcgt tcttcgaggc cgcgctcgcc gcgggcgcga
     1381 ccacccgcgg cgaacccggc gtctggatcc agtactcgaa ccggtactac ggggcgttcg
     1441 tgaacgacct ccacggcaac aatgttgaag ccgtgttcca cagtccggtg ccgatcacgg
     1501 acgccccgga acgcgggggc gctccctagg cgagtcggcg agcaagaaca cctgcggtgc
     1561 agcgatctca gtcgatcctc gggtatctcg cccgttctcg tttcactgcc gcacgtcccg
     1621 ggtagggaca agatgaagtc cccaacaggc acgacatcga cacctgtgaa ggaggtgccc
     1681 aatgaggact gatctctgga actacccgga aggaacgacc acacccctgg acatccaagg
     1741 gttcaaggtt cgggcaacgg acggtgagat cggcaagatc gacgaggcca cgtacgacgt
     1801 tggttcggcg ttcatcgtcg tcgacaccgg tccgtggatc ctcggccgga aggtactgat
     1861 cccggccggg atggccgagc ggatcgacct cgatcgacgt gaggttgagg ttcggctcac
     1921 gaaggaccag atcaagaacg cgcccgagct cgacgaggtg accgactacc ggtcggacac
     1981 gtatcgcgag caggtcggga cctactacag cacctacagc taggtccgac gttccgtgga
     2041 gcgaggcgag ggcatccgcc ctcgcctcgt tccgtctagt gggagcctcg caccgccgac
     2101 cgctaggtca gctcctcctt cgacgccgcg aacgcctcga cggcgaacga caggtcctcg
     2161 cgcgtgtgcg ccgccgagac ctgcgcgcgg atccgggcct tccctttggg gaccaccggg
     2221 taggagaacc cgatcacgta gacgcccctc tcgagcat
//
LOCUS       DASUVE010000208         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_288051, whole genome shotgun sequence.
ACCESSION   DASUVE010000208 DASUVE010000000
VERSION     DASUVE010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288051"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VF036_04525"
     CDS             complement(<1..906)
                     /locus_tag="VF036_04525"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6264527.1"
                     /translation="MDLLAVELGIDPVDLRRRNLIGPDEFPFTTVTGVTYDTGDYART
                     LDEALRLVAYDDLRAEQDSRRARGDEVCLGIGVAMYVEITAFGGREFAAVEIGPDARV
                     TVAVGTTPAGHGHETSFAQLASTSLGVPFEDVTVVHSDTGLVARGEGTWGSRSLQIGG
                     SSVYEQAEAVVEAGRRMAAAMLEVDEDDVERIEGGFAVAGAPDRSVGWSEVVASDDGA
                     IRAEGRFRQRGSTFPFGAHVAVVEVDTATGAVRLLRHVAVDDCGRILNPVLVRGQQHG
                     GLGQGIAQALFEEVRYDESGNPVTST"
     gene            complement(846..2039)
                     /locus_tag="VF036_04530"
     CDS             complement(846..2039)
                     /locus_tag="VF036_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6264528.1"
                     /translation="MATAPPSMFGVPALRSEDPRFLRGEGRYLDNVEIPGALHAVFVR
                     SSMPHARVDGLDVTGCLASPGVVGAYTARDLELAPLPPSGTVEGAADGELEGLFSREP
                     IAHDAVRFVGEILAVVVAESVGEAIDAAELAAVDYEPLATVVDPEAAIADGAPLLWPE
                     FGTNVAHSFEATGPDDPLGGADVVVRGRFVNQRVAPAPLETNGIAVVPEGDGSFTVWV
                     STQIPFDVRDDLAETLGVGRDRVRAITPDVGGAFGAKLQVYAEYHAVAAIAARLGRPV
                     RWQETRSESMVSLTHGRAQIQTVELGATRDGTLVGLRAELLADMGAYPIGAFMPGTTQ
                     EMLSGVYRIPRIACSGRSVVTNTTRSLRTEARADPRRRRSSNARWTCSQSSSGSIPST
                     CDGGT"
     gene            2074..>2309
                     /locus_tag="VF036_04535"
     CDS             2074..>2309
                     /locus_tag="VF036_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264529.1"
                     /translation="MRRMGILAASVALSLAISIAGLRGHGTRPALAGGFRAVRVVSGL
                     ASPVGFTFTPGGRLVYLERHTGWLRFRNLQTDVDV"
BASE COUNT          338 a          878 c          770 g          323 t
ORIGIN      
        1 cgtggacgtc accgggttgc cgctctcgtc gtaccgcacc tcctcgaaca acgcctgcgc
       61 gatcccctgc ccgagcccgc cgtgctgctg ccctcggacg agcaccggat tgaggatgcg
      121 accgcagtcg tcgacggcga cgtggcggag caggcgcacg gccccggtcg cggtgtccac
      181 ctccacgacc gccacgtgcg cgccgaacgg gaacgtcgat ccgcgctgcc ggaagcgccc
      241 ctccgcgcgg atcgctccgt catcgctcgc gacgacctcg ctccagccga cgctccgatc
      301 gggggcgccg gcgacggcga atccgccctc gatccgttcc acgtcgtcct cgtccacctc
      361 gagcatcgcg gccgccatcc gacgaccggc ctccacgacg gcttcggcct gctcgtagac
      421 ggacgagccc ccgatctgca aggaacgcga cccccaggtc ccttccccgc gcgcgacgag
      481 ccccgtatcg gagtgcacga ccgtgacgtc ctcgaacgga accccgagcg acgttgatgc
      541 gagttgggcg aacgaggtct cgtggccgtg accggccggt gtggtcccga cggcgaccgt
      601 gacgcgcgca tcgggaccga tctccaccgc cgcgaactcc ctgccgccga acgcggtgat
      661 ctcgacgtac atcgccacgc cgataccgag gcagacctcg tcgccccgcg cccgccgcga
      721 atcctgttcc gcgcggaggt cgtcgtacgc gaccagtcgg agtgcctcgt cgagcgtcct
      781 cgcgtagtca ccggtgtcgt acgtgacgcc agtgaccgtc gtgaacggga actcgtcggg
      841 tccgatcagg ttcctccgtc gcaggtcgac gggatcgatc ccgagctcga ctgcgagcag
      901 gtccatcgcg cgttcgacga gcgccgtcgc ctcgggtcgg cccgcgcctc ggtacggagc
      961 gaccgggtcg tgttcgtcac cacgctcctg ccgctgcacg cgatccgagg gatccggtag
     1021 acgccgctca acatctcctg cgtcgttccc ggcatgaacg caccgatcgg gtaggccccc
     1081 atgtcggcca ggagctcagc gcggagtccg acgagcgttc cgtctcgcgt ggcgccgagc
     1141 tccacggtct ggatctgggc gcggccgtgc gtgagcgaaa ccatcgactc cgaacgcgtc
     1201 tcctgccacc ggacgggccg ccccaggcgc gcggcgatcg ctgccacggc gtggtactcc
     1261 gcgtacacct ggagcttggc gccgaacgcg ccgcccacat cgggcgtgat cgcccgcacg
     1321 cgatcacgcc cgacccccag ggtctcggcg agatcgtcgc gcacgtcgaa ggggatctgc
     1381 gtcgacaccc acaccgtgaa cgagccgtcc ccctcgggca ccaccgcgat cccgttcgtc
     1441 tccagcggcg ccggcgccac ccgctggttc acgaaccggc cgcgaaccac gacatcggcg
     1501 ccgccgagcg gatcgtcggg accggtggct tcgaacgaat gcgcgacgtt ggtgccgaac
     1561 tccggccaga gcaacggcgc accgtccgcg atcgccgcct ccgggtccac cacggtcgcg
     1621 agcggctcgt agtccacggc ggcgagctcc gcggcgtcga tcgcctcgcc gacgctctcc
     1681 gcgaccacga cggcgaggat ctccccgacg aagcgcacgg cgtcgtgcgc gatgggttcc
     1741 cggctgaaca gcccttcgag ctcgccgtcc gcggccccct cgacggtgcc ggagggcggc
     1801 aagggagcca gctccagatc ccgtgcggtg taggcaccca cgacccccgg ggacgccaga
     1861 cagccggtca cgtcgaggcc atcgacccgc gcgtgcggca tgctggaacg gacgaagacg
     1921 gcgtgcaggg cgccggggat ctcgacgttg tcgaggtatc ggccctcacc tcggaggaac
     1981 cggggatcct ccgatcggag agccggcacg ccgaacatgg agggaggcgc cgtcgccacg
     2041 gcgccgatac tggcatcgga aggagcgctc gacatgcgga ggatggggat cctggcagct
     2101 tcggtggctc tgtccctggc catctcgatc gccggcctca ggggccacgg gacccggccc
     2161 gccctcgcgg gcggcttcag ggcggtccgc gtcgtatccg ggctggcgag ccccgtcggc
     2221 ttcacgttca cgcccggagg ccgcctcgtc tacctcgagc ggcacacggg atggctccgc
     2281 ttccgcaatc tccagaccga cgtggacgt
//
LOCUS       DASUVE010000209         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1041209, whole genome shotgun sequence.
ACCESSION   DASUVE010000209 DASUVE010000000
VERSION     DASUVE010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041209"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1959)
                     /locus_tag="VF036_04540"
     CDS             complement(<1..1959)
                     /locus_tag="VF036_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX6264530.1"
                     /translation="MIGLTIVSLLVVPFVPAGAAPRSHNPPAVGILTDSDGDHVDEAL
                     ERRLRRSEGTTRHAVVVATDGSLSLARAHRVAGSFSVGRRLEIVGGFSAKLSAGQIRR
                     LASTPGVLRIDHDAVIRATMDAARSDYGVEAARADLGLTGAGITVCILDTGADPDHEQ
                     LDSKNIVWHDFVGTAASPVDDHGHGTHVASIAVGDGVGGPNAARLGGVAPAAGLWVGK
                     VLNAQGSGSESGIIDAIDWCVDSPEVDVISMSLGSELPSDGSDVLGIAANNAVASGKI
                     VVAAAGNAGDAPDSMASPGAASDVIAVGAASSWSAAPGAVNHANGIYIAPFSARGGPT
                     FAADPKPDVIAPGVNVRAAAANTGSGYTTLSGTSMAAPFAAGSIALALEAWAGSSPTP
                     AAVQNAVEASAEDYGPVGKDPDWGAGLIDVLALTSQAAGVSGATVFPTHQHLSGTVPD
                     HGEWTHEFELGPDDLGVPIAVSIVLDGSCEDFLGFGCLFGWAPDIDAELVDPTGELLD
                     VSVCAAIESGDPSPGPCAIGRQETLHAMPSIAGTYLIRLFPFGGSPNDGLGGAFDLDL
                     STGPVGEDTTPPPPPPPPPPPPSIHVGDLDDVSITLSSTRWRARATIRVHDGTHAAES
                     GVLVRGRFGPNGSTLSCTTGAAGACTLKR"
BASE COUNT          312 a          704 c          713 g          287 t
ORIGIN      
        1 ccgcttcaac gtgcaggctc ccgcagcccc ggtcgtgcac gaaagggtcg acccgttcgg
       61 cccgaaccgc cctcgcacga ggacacccga ctccgcggcg tgcgttccgt cgtgcacgcg
      121 gatggtcgcg cgggcacgcc atcgtgtgga cgacagggtg atcgacacgt catcgagatc
      181 cccgacatgg atcgagggtg gcggtggggg tggcggtggc ggtgggggcg gggtggtgtc
      241 ctcgccaacc gggcccgtcg acaggtcgag gtcgaacgct ccaccgagtc cgtcgttcgg
      301 tgagccgccg aacgggaaca ggcggatcag gtacgttccc gcgatcgatg gcatcgcgtg
      361 cagcgtctcc tgacgcccga tcgcgcatgg tcccggagac ggatcccccg actcgatcgc
      421 cgcgcacacg ctgacgtcga gcagctcgcc cgtggggtcc acgagctccg cgtcgatgtc
      481 gggagcccat ccgaagaggc acccgaaccc gaggaagtcc tcacacgatc cgtcgagcac
      541 gatcgagacg gcgatcggca caccgaggtc gtccgggccg agctcgaact cgtgcgtcca
      601 ctcaccgtgg tccgggaccg tgccggacag atgctggtgg gtcgggaaga ccgtggctcc
      661 cgagacccct gccgcctggg aagtgagcgc caggacgtcg atcagaccgg caccccagtc
      721 gggatccttg cccaccggcc cgtagtcctc ggccgatgcc tcgaccgcgt tctggaccgc
      781 ggcgggcgtc ggtgacgatc cggcccacgc ttcgagcgcc aatgcgatgg agcctgcggc
      841 gaagggcgcg gccatcgagg tcccgctgag ggtcgtatag ccggatccgg tgttcgctgc
      901 tgcggctctc acgttcacgc cgggagcgat cacgtcgggc ttcggatccg cagcgaacgt
      961 cggaccgccg cgggcggaga agggagcgat gtagatgccg ttcgcatggt tgaccgcgcc
     1021 gggcgccgcc gaccaggacg aggccgcgcc cacggcgatc acgtccgagg cggcgccggg
     1081 cgacgccatg gaatccggtg catcgcccgc gttccccgcc gcggccacca cgatctttcc
     1141 gctcgccacc gcgttgttcg cggcgatgcc gagaacgtcg gagccgtccg acggcagctc
     1201 cgacccgaga ctcatcgaga tcacatcgac ctccggtgag tcgacgcacc agtcgatcgc
     1261 gtcgatgatc ccggattcgc tcccggaccc ctgggcgttg aggaccttgc cgacccagag
     1321 tccggccgcg ggagcgaccc caccgagcct ggccgcgttg gggccgccga cgccgtcacc
     1381 gacggcgatc gaggcgacgt gggttccgtg cccgtgatcg tcgaccgggc tcgcggcggt
     1441 ccccacgaag tcgtgccaga cgatgttctt cgagtcgagt tgctcgtggt cgggatcggc
     1501 gcccgtgtcg aggatgcaga ccgtgatgcc cgcccccgtc agtccgagat ccgcccgggc
     1561 cgcctcgacg ccgtagtcgg atctcgccgc atccatcgtg gccctgatga ccgcgtcgtg
     1621 gtcgatccga aggacgccgg gcgtggacgc cagccgacgg atctgtccgg cggagagctt
     1681 ggccgagaat ccaccgacga tctccaacct gcgaccgacg gagaacgagc ccgcgacccg
     1741 atgggcacgg gcgaggctga gcgacccatc cgtggccacg accacggcat ggcgggtcgt
     1801 cccctcggac ctgcggaggc gtcgctcgag cgcttcgtcc acatggtcgc cgtccgagtc
     1861 ggtgaggatg ccgacggccg gagggttgtg cgaacgtggg gcggctcccg ccggcacgaa
     1921 gggcacgacc agcagcgaca cgatggtgag accgatcacg acacgtcgcg cgtgcagggg
     1981 aaccctcctt cccgggaaca cgctgagcct gcgggg
//
LOCUS       DASUVE010000210         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_852094, whole genome shotgun sequence.
ACCESSION   DASUVE010000210 DASUVE010000000
VERSION     DASUVE010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852094"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VF036_04545"
     CDS             complement(<1..918)
                     /locus_tag="VF036_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264531.1"
                     /translation="MDATDPRGEVVRHDEGLRDIHRRISSQSRLDEGGAATATTQPRE
                     WRRGRAFAVLSGWGVTAVGGTDARSWLHDLVTADVEGLPDRASRRCLLLTPTGRIRAD
                     FHIAAVDGSYLLLQAPDQPDRVDVILRPYVLSSDVEVTDLGDRSEVVAVLDGSAVEDD
                     AGTVVLSPSVLGPGCDIVVPSGDASARLRDRLRADGLTEADAGDLEARRIRRGEPRMG
                     VDFGADALPAEAGLEDTIDFTKGCFLGQESVAKVRNLGHPPRVLLHVRSRATIARGAR
                     VVAGGTVVGEVTSAVEDADGVAAIVQVRWD"
     gene            856..1989
                     /locus_tag="VF036_04550"
     CDS             856..1989
                     /locus_tag="VF036_04550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6264532.1"
                     /translation="MDIPKTLVVTNDFPPRVGGIQRTLESLCRELPPDRISVIAPSCE
                     GSERFDASVPFTVVRERRRFVWPSPGVARRLHAEVERSGAEVVLFGDAFPLALLGSQL
                     KDRGTPSVVLAHGFDYWLSTMPVAHAVMKRMTTPSSRVAGCSEFISRRVRTAVPKHVP
                     VSVLHPGADVERFRPDLPTADIRERHGLGDRPLIVCVSRLVPRKGQDVLIRAMRSIQR
                     RVPEAGLLIVGSGPFETTLRSLAEHAPSRSVTFVGEVSEEDLPRYYAVGDVFAMPCRT
                     RLAGLEVEGWGNVFIEAAACGRPVVVGDSGGSREALVHGATGLLVDGAEVGGVADAVA
                     TLLEDPAYARRLGKAGRARVEREHTWARVAERLAGWLREASAA"
     gene            2098..>2416
                     /locus_tag="VF036_04555"
     CDS             2098..>2416
                     /locus_tag="VF036_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264533.1"
                     /translation="MSADAEWCGQCFSSLRASTPEPAAAAVPVVARPREGPDDETQGE
                     HEAPAKLTWPCPTCGSQNDLTLDLCSVCGTSFGALLREERATPTVSPRDAFLWSLTFP
                     GVGH"
BASE COUNT          329 a          876 c          861 g          350 t
ORIGIN      
        1 atcccaccgg acctggacga tcgcggcgac cccgtccgcg tcctccacgg cgctcgtcac
       61 ctcgccgacg acggttccac cggcgacgac ccgggcgccc cgggcgatcg tagccctgga
      121 ccggacgtgg aggaggacgc gcggcgggtg tccgaggttg cggaccttcg ccacggactc
      181 ctgtccgagg aaacatccct tcgtgaagtc gatcgtgtcc tccagccccg cctccgcggg
      241 gagggcgtcc gccccgaaat cgacgcccat ccgcggctcc ccgcggcgga tccgccgggc
      301 ctcgaggtct ccggcgtccg cctcggtcag cccatctgct cggagacggt cccggagccg
      361 tgcggaggca tcccccgagg gcacgacgat gtcgcacccc gggccgagga cggacgggga
      421 caggacgacg gtcccggcgt cgtcctcgac ggcggagccg tcgaggacgg ccaccacctc
      481 cgagcggtca ccgagatcgg tcacctccac gtccgacgag agcacgtacg gtcgcaggat
      541 cacatccacc cggtcgggct ggtccggagc ctggaggagg aggtaggaac cgtccaccgc
      601 ggcgatgtgg aaatccgcgc ggatccgccc ggtgggggtg aggaggagac atcgtcggga
      661 cgctcggtcg ggcagcccct cgacgtccgc cgtgactagg tcgtgcagcc acgaccgggc
      721 atcggtgccc cccacggcgg tgacgcccca cccggagagc accgcgaagg cccgacctcg
      781 tcgccactcc ctcggttgcg tcgttgccgt cgcggcacct ccctcatcga gcctgctctg
      841 ggaggatatc cgccggtgga tatcccgaag accctcgtcg tgacgaacga cttccccccg
      901 agggtcggtg gcatccagcg aacgctggaa tcgctctgcc gggagctgcc ccccgaccgg
      961 atctcggtca tcgcgccgtc ctgcgaggga tcggagcgct tcgacgcgtc ggttcccttc
     1021 accgtggtcc gcgagcgccg gcgattcgtc tggccgtcgc ccggcgtggc ccgcaggctg
     1081 cacgccgagg tcgagcgatc gggcgcggag gtggtgctgt tcggcgacgc gttcccgctc
     1141 gccttgttgg ggtcgcagct caaggatcgg ggcacgccct ccgtcgtcct cgcgcacggt
     1201 ttcgactact ggctctcgac gatgccggtc gcccatgccg tcatgaagcg gatgacgacg
     1261 ccctcgtcgc gggtggccgg gtgcagcgag ttcatctccc gccgggtacg gaccgccgtg
     1321 ccgaagcacg tgccggtctc ggtgctccat ccgggcgccg atgtcgagcg gttccgcccg
     1381 gatctgccca ccgccgacat cagggaacgg cacgggctgg gcgaccgccc gctgatcgtc
     1441 tgcgtgagcc ggctcgtccc gcggaagggg caggacgtgt tgatccgagc gatgcgctcg
     1501 atccaacgac gggtgccgga ggcggggctg ctgatcgtcg gctccgggcc cttcgagacg
     1561 accctccggt cgctcgcgga gcacgcgcct tcgcgctcgg tcacgttcgt gggcgaggtg
     1621 tccgaagagg acctgccacg ctactacgcc gtcggagacg tcttcgcgat gccgtgccgg
     1681 acccgcctcg ccggactcga ggtggagggc tgggggaacg tcttcatcga ggcggccgcg
     1741 tgtggtcgtc cggtggtcgt cggcgactcc ggcgggtccc gcgaagccct cgtccacgga
     1801 gcgaccgggc tgctcgtcga cggggcggag gtgggcgggg tggcggacgc cgtcgcgacg
     1861 ctgttggagg accccgcgta cgcccgacgg ctcgggaagg cgggccgggc ccgtgtcgaa
     1921 cgcgagcaca cctgggcccg ggtcgcggaa cgcctcgcgg gatggctccg cgaagcgagc
     1981 gccgcctgac gcgaccgatc gtcctcccgc ggcgaaggag cacctcccaa ccgggcgcgg
     2041 taccgacggt acccttgccc cgtgcaggat caccgctgtc cgaattgcaa cgcgctggtg
     2101 agcgcggatg ccgaatggtg cggacaatgc ttctcctcgc tccgagcctc gaccccggag
     2161 ccggctgcgg ccgccgtgcc cgtggtcgca cgcccacggg aaggaccgga cgacgagacg
     2221 caaggggagc acgaggcacc ggcgaagctc acgtggccgt gcccgacgtg cgggtcgcag
     2281 aacgacctca ccctcgacct ctgcagcgtc tgcggtacct ccttcggtgc acttctgcgg
     2341 gaggaacggg cgaccccgac cgtctcccct cgcgacgcct tcctgtggtc gctcacgttc
     2401 cccggcgtcg gacacg
//
LOCUS       DASUVE010000211         3766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1042082, whole genome shotgun sequence.
ACCESSION   DASUVE010000211 DASUVE010000000
VERSION     DASUVE010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042082"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..788
                     /locus_tag="VF036_04560"
     CDS             306..788
                     /locus_tag="VF036_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006672866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEX6264534.1"
                     /translation="MTRSNAADVRKKAEHADARLRPFLARIGIPMLRISVGVVFLWFG
                     LLKIFDVSPVSGLVAKTIYWFDPDLVVPALGAVEVFVGLCLLAGRLMRLALPLLVLQM
                     AGTFLVLVLLPEIAFRDGNPLLLTVEGEFVIKNLVLFSAALVIGSRLAPLGEGGTGSA
                     "
     gene            817..2199
                     /locus_tag="VF036_04565"
     CDS             817..2199
                     /locus_tag="VF036_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX6264535.1"
                     /translation="MDTARTVDIDLEWVEELTKRETEALDRRHARSLSYREEAKRFLP
                     GGVSSSWQSWPPHPIYVRRGEGAHVWDIDGNEYVDLHNGYGVMVMGHAHPKVVEAVQR
                     RVAEGTHFAQPTEEALPVAENLAERYRLPQWRFGNSGTEATLDACRISRALTGRSKIL
                     KIEGTYHGHHDSLMVSVFPSEEQAGSRDRPVAVPQTKGLPKEFVDLVVNVPFNDLVAA
                     KAAFAEHAGEFACMIVEPAMTNCGLVLPDPGYLAGLKELCRADGALFVFDEVKTGFTL
                     AWGGAVEAFGVVPDLVCLAKALGAGLPCGAIGGTDEAMGMVISGELDQQGTFNGNPLT
                     MAAAKANLLEVMTPEAYGRFEELNAILVRSLEACTKHGLPANVKLLGAKGSVEFRDQP
                     IHEYRDLWDIDDRVPQLAWLWQLNRGVFKSPGSKWESWTTSIVHSDADVQRYVDNLEE
                     FAATVTNPAA"
     gene            complement(2218..>3766)
                     /locus_tag="VF036_04570"
     CDS             complement(2218..>3766)
                     /locus_tag="VF036_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX6264536.1"
                     /translation="TGGTNGGWTLLSSPVNGTDGFGSYFFCQFQCGYDMFVESPPGQP
                     DTVWIGGAMNYDELPVFGGNGRSNGRAVMRSTDAGVSFTDMTNDAQNPPLGMHPDQHA
                     IVFASDPDIAIVGSDGGVIRTDGDYVDDSDECASRPIEGPDLVDCENWLSAVPSLLTT
                     MNDGLATIQFQSLSANPDDPLGDLMGGTQDNGTWLFSGGPEWLETIDGDGGQSGYGAE
                     NSDIRVHTYFDATPDVSFDAGEPGSWNWIGDPLQKSKEQRSFYVPLITDPVVGGAMFT
                     GMESIWRTQNNGGPQAFLEENCNEYTGTFQHVCGDWKPIGSGTSGNLTSTFYGRDKLG
                     HYVVATERSGGDTGTLWAGTRVGRVFVSKNANAPRQQVSYRRIDTPAQPERFVSGIAI
                     DPTNPNHALVSFSGYEAYTPGQPGHVFEVTFHPGTGNATWKDISANIGDQPVTDVAFD
                     DQTGDVYVSTDFNVLRLKDGGSVWVEAATGLPPVATYGLTIDTSDAGRVLYAATHGRG
                     AWSVALP"
BASE COUNT          581 a         1273 c         1269 g          643 t
ORIGIN      
        1 gaacctcgcg gagcgccgct ccctgcggga ggacgtgcgc gatctcgtcc gccgtccaac
       61 gggggcagca ggaggcaaca cgaagagttc atccgacatg tccattcttg ggtctatcca
      121 cccccttcct acgccaaacc tgagctcgag gtcgggcaca tcttgagcct cgaagggcaa
      181 gcattccata gatgaggtag ggaggaccac ccactcagcc ctcgcctgag ggtttcgtca
      241 ttgaggatcg atcgatgaca ccgacgcaca ggcccgacaa ccgcgagggc ggctaccatc
      301 tttgggtgac ccgatcgaac gccgccgacg ttcggaagaa ggccgagcac gccgacgccc
      361 ggctccggcc gttcctggcg cggatcggca tccccatgct ccggatctcc gtcggcgtgg
      421 tgttcctctg gttcgggctg ctcaagatct tcgacgtctc gccggtgtcc ggcctcgtcg
      481 cgaagacgat ctattggttc gatcccgacc tggtcgtccc ggccctcggc gccgtggagg
      541 tcttcgtcgg gctctgtctc ctggcgggca ggttgatgcg gctcgccttg ccgctcctcg
      601 tgctccagat ggccggcacg ttcctcgtgc tcgtcctgct cccggagatc gcgttccgcg
      661 acgggaaccc tctcttgctc accgtcgagg gcgagttcgt catcaagaac ctcgtcctgt
      721 tctcggcagc gctcgtgatc gggtcccggc tcgcgccctt gggtgaaggc gggaccggca
      781 gcgcctgagc cggcggacgg cgtaatctcc tcggcgatgg ataccgctcg gaccgtcgac
      841 atcgacctgg agtgggtcga ggagctgacg aagcgcgaga ccgaggcgct cgaccgaaga
      901 cacgcccgct cgctctcgta ccgggaggag gccaagcgct tcctgcccgg gggcgtctcc
      961 tcctcgtggc agtcgtggcc accgcatccg atctacgtgc gacgtggcga gggcgcgcac
     1021 gtgtgggaca tcgacgggaa cgagtacgtc gacctccaca acggctacgg cgtgatggtg
     1081 atggggcacg cccatccgaa ggtggtggaa gcggtgcagc gccgggtcgc cgaggggacg
     1141 cacttcgccc agccgacgga agaggcgctc ccggtcgcgg agaacctggc ggagcgctac
     1201 cggttgccgc agtggcgctt cggaaactcc ggcaccgagg cgacgctcga cgcgtgccgg
     1261 atctcccgcg ccctgaccgg ccgctcgaag atcctgaaga tcgagggcac gtaccacggc
     1321 caccacgact cgctgatggt cagcgtgttc ccgtcggaag aacaggcggg ctcacgcgac
     1381 cgtcccgtcg cggttccgca gacgaagggg ctgcccaagg agttcgtcga cctcgtcgtg
     1441 aacgtcccgt tcaacgacct cgtggcggcg aaggcagcgt tcgccgagca tgccggcgag
     1501 ttcgcgtgca tgatcgtcga gccggcgatg acgaactgcg ggctcgtcct gcccgatccc
     1561 ggctacctcg ccggcctgaa ggagctctgc cgggcggacg gcgcgctgtt cgtgttcgac
     1621 gaggtgaaga ccggcttcac gctcgcgtgg ggcggtgccg tcgaggcgtt cggcgtcgtg
     1681 ccggacctcg tctgtctggc gaaggcgctc ggcgcgggcc tcccatgtgg cgcgatcggg
     1741 gggacagacg aagcgatggg catggtgatc agcggcgagc tcgaccagca gggcacgttc
     1801 aacggcaacc cgctcacgat ggccgcggcg aaggcgaacc tgctggaggt gatgacgccg
     1861 gaggcgtacg ggcgtttcga ggagctgaac gcgatcctcg tccgcagcct ggaggcgtgc
     1921 acgaagcatg gcctcccggc caacgtgaag ctgttgggcg ccaaggggtc cgtggagttc
     1981 cgcgaccagc cgatccacga gtaccgcgac ctgtgggaca tcgatgaccg cgtgccgcag
     2041 ctcgcgtggc tgtggcagtt gaaccgaggc gtgttcaagt cacccgggtc gaagtgggag
     2101 agctggacca cctcgatcgt gcactccgac gccgacgtcc agcgctacgt ggacaacctc
     2161 gaggagttcg cggccacggt gacgaacccg gccgcctgac ctgccgcggt cgccgcttta
     2221 cggcagcgcc accgaccagg cccctcggcc gtgcgtggcc gcgtagagca cccggcccgc
     2281 gtccgacgtg tcgatcgtga ggccgtacgt ggccaccggc ggaagccccg tagccgcctc
     2341 gacccacacg ctgccgccgt ccttgagccg aaggacgttg aagtctgtcg acacgtagac
     2401 gtcgcccgtc tggtcgtcga acgcaacgtc ggtgaccggc tggtcgccga tgttggcact
     2461 gatgtccttc caggtcgcgt tcccggtgcc cgggtggaac gtcacctcga acacgtggcc
     2521 gggctggccg ggcgtgtacg cctcgtatcc ggagaacgag acgagtgcgt ggttcgggtt
     2581 ggtcggatcg atcgcgatcc cgctcacgaa ccgctccggc tgtgcgggcg tatcgatcct
     2641 ccggtagctc acctgctgtc ggggggcgtt cgcgttcttc gagacgaaca cgcgaccgac
     2701 ccgggtcccc gcccagagcg tcccggtgtc gccgccggag cgctcggtgg caacgacgta
     2761 gtgcccgagc ttgtcgcgtc cgtagaacgt cgacgtcagg ttgccgctcg tgcccgaacc
     2821 gatcggcttc cagtccccgc agacgtgctg gaaggtgccc gtgtactcgt tgcagttctc
     2881 ctcgaggaag gcctgcgggc caccgttgtt ctgagttcgc cagatgctct ccatcccggt
     2941 gaacatcgcc ccaccaacga ccggatcggt gatcaggggc acgtagaacg agcgctgctc
     3001 cttgctcttc tggagcggat caccgatcca gttccagctc ccgggctccc cggcgtcgaa
     3061 gctcacgtcg ggcgtcgcgt cgaagtacgt gtggacgcgg atgtcgctgt tttccgcgcc
     3121 gtagccggac tgaccgccgt cgccgtcgat cgtctccagc cactccggcc cccccgagaa
     3181 gagccacgtc ccgttgtcct gcgtcccgcc catcagatcg cccagcgggt cgtcggggtt
     3241 cgccgacagg ctctggaact ggatcgtcgc gaggccgtcg ttcatcgtgg tgagcaagct
     3301 cggcaccgcg ctcagccagt tctcgcagtc cacgaggtcc ggcccttcga tcggcctcga
     3361 tgcacactcg tcggagtcgt cgacgtaatc cccgtcggtg cggatcacac caccgtcgga
     3421 ccccacgatc gcgatgtcgg gatcggaggc gaacacgatc gcgtgctggt cggggtgcat
     3481 gccgagcggg gggttctgcg cgtcgttcgt catgtcggtg aacgagacgc ccgcatcggt
     3541 ggagcgcatc acggcacgcc cgttcgagcg gccgtttccc ccgaagaccg gcagctcgtc
     3601 gtagttcatc gcgccgccga tccacaccgt gtcgggctgc cccggcggtg attccacgaa
     3661 catgtcgtac ccgcactgga attggcagaa gaagtacgaa ccgaacccat cggtgccgtt
     3721 gacggggctc gagagcagcg tccagccgcc gttcgtcccg cccgtc
//
LOCUS       DASUVE010000212         4841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_289260, whole genome shotgun sequence.
ACCESSION   DASUVE010000212 DASUVE010000000
VERSION     DASUVE010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289260"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..992
                     /locus_tag="VF036_04575"
     CDS             237..992
                     /locus_tag="VF036_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX6264537.1"
                     /translation="MISLDGIRRVPFGHFTIPDDGDQRAGEKVVVCGYLIDHPDGLVL
                     LDTGIAEGHEEVEELYHPVRRPLDEALATAGVTTGDIRIVVNCHLHLDHCGGNPRFPD
                     TPIFAQEIEFDAAHREEDYPIVGLYDFPEAQYELHGGEADVTTGVRVVPTPGHTPGHQ
                     SLVVETDAGRVVLAGQAVNGATDYGRAHYASELRRSGSEETVIVPEWINGSRSSTPSW
                     CCSPMTTPLGRRSCDPSQEIVMRPSSSMGKVGL"
     gene            complement(941..1372)
                     /locus_tag="VF036_04580"
     CDS             complement(941..1372)
                     /locus_tag="VF036_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018067853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6264538.1"
                     /translation="MSVPARINIVTLGVDSVARSKAFYEALGWELAGSVGDEICWFRT
                     SDSYLGLFGREDLARDAGLRSEPTAEFGGITLAINVGSEAEADAAFEAAVAAGARIMR
                     PAERTDWGGYSGYFADPDGHPWEIAYNPTFPIDEDGRITIS"
     gene            1442..1879
                     /locus_tag="VF036_04585"
     CDS             1442..1879
                     /locus_tag="VF036_04585"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX6264539.1"
                     /translation="MPAVRLNPMLRVMWKLHRFVLRVSGGRVGSRMGSMKVLLLETTG
                     HKTGESRTVGLSHLEFDGRYFVMASYAGEDRDPAWAKNLRAHPRATVSIGRRSTPVVA
                     RALEGAEREAMFDRFVEADAAYREYRERTTRDIPVFELRPEGA"
     gene            1885..2799
                     /locus_tag="VF036_04590"
     CDS             1885..2799
                     /locus_tag="VF036_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX6264540.1"
                     /translation="MPSITDIEGLTVGHWTDEVALTGCTVVLPPPGTIASCEVRGGAP
                     GTRGTDILQPGTIIEVAHAIVLTGGSAFGLASAGGVERYFEERGIGSEIGPARVPTVP
                     AAVIFDLGVGDPSRRPGADEGYAACIAASADVREGRVGVGTGATVAKLWGPERAVPGG
                     VGTWAVHDGELVVGALVVVNAVGEIVDEDGSILVGPRLEHGERREDLVEQLGSSGAAN
                     TTLGVVATNAVLSKEDVRRLASIGNDALDVAIRPAHTIYDGDTVFALATQQVHASFEQ
                     VAALVEPGVTTAIRRAVTRDDGSGAAGA"
     gene            complement(2779..3672)
                     /locus_tag="VF036_04595"
     CDS             complement(2779..3672)
                     /locus_tag="VF036_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX6264541.1"
                     /translation="MNELRTRAFRLPFQAPFAADTLLSFLGARAVPGIEETRDGTYVR
                     SVRQPSGAAVAAMSPRSDHVIVRVSSEGSPDLDDLRERARRAFDLDADAATIDTTLAA
                     DPTMIPLVTRIPGIRVPGTFDGFELVVRAIYGQQVSVAGARTSLGRLVSAAGTPLERP
                     SGGITHLFPTAERVAEVPLEVFGMPRARAETIRRVALLVADGELDLSGEAPHEETLRR
                     LGDVPGIGPWTLAYVSMRALRDPDAFMAGDLGVRKGFEALGLASMQPAMLARAERWRP
                     WRAYAVMHLWHQQRVSGAGRA"
     gene            3760..>4841
                     /locus_tag="VF036_04600"
     CDS             3760..>4841
                     /locus_tag="VF036_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264542.1"
                     /translation="MARVILALEAPEVTEEVMHVLDRSGRARVVATAGDDRQLAEAVR
                     QLEPDVVVAEPSLLSGARACGVVLAIDVRESVSSLRTAIRAGAAGYFLWPKERDDLLV
                     AAAAAVRRAQQTARRGAVVAVHGGRGGAGVTFVATHLAAAFARRGSAILIDADPIFGD
                     VAQALGAPTGEEAEPTHTFADAAALGEDLGPEQLRSALWRHESEVDVLLPPPPEEAIR
                     LGPEDLRLVTEVAAGVADVVVVHLPRALGAMTRAGAETSDSLVEVLTLDVVSFRATSR
                     VLEACAPLHLEGRVSFVINKAARSEITPGDVHRVFGEPARAIIPHDRAVRSAQERNRL
                     LPPKGLMARRFDRLADALSAPVVEEMA"
BASE COUNT          707 a         1654 c         1740 g          740 t
ORIGIN      
        1 tcgtccacga cgccatcggt cggttcacag gtggaggtcg gctcgtccgc cggatcgtcg
       61 aaggcgtcac cggcgagtgc gaatccggcg gagagcaccg cgatgaccac gcccacgatg
      121 gcgacgaagc gcaggcgctc ccctttgtgc atgccccagt atcgaccgag ttgggccgaa
      181 atcttgagcc ttcgttcagg tagaggtgga tcaatctccg cctagggtgc cgtgccgtga
      241 tctccctcga cggcatccgt cgtgtcccgt tcggtcattt cacgatcccc gacgacgggg
      301 atcagcgcgc cggcgagaag gtcgtggtct gcggctacct gatcgaccac ccggatgggc
      361 tcgtgctcct cgacaccggt atcgccgagg gacacgagga ggtggaggag ctctatcacc
      421 ccgtgcggcg tcccctggat gaggcgctcg ccacggccgg cgtgaccacc ggggacatcc
      481 ggatcgtcgt gaactgccac ctccaccttg atcactgcgg tgggaaccct cgcttcccgg
      541 acacgccgat cttcgcgcaa gagatcgagt tcgatgccgc gcaccgcgag gaggactacc
      601 cgatcgtggg cctctacgac ttccccgagg cccaatacga gctccacggc ggcgaggccg
      661 acgtcacgac gggggtccgc gtggtgccga cgccgggcca cacgccgggt catcagtcgc
      721 tcgtcgtgga gaccgacgcc ggccgggtgg tgctcgccgg ccaggcggtc aatggggcga
      781 ccgactacgg acgcgcccac tacgcgagcg agctccggcg gagcggctcg gaagagacgg
      841 tcatcgtgcc cgagtggatc aacggctcca ggagttcgac cccgtcctgg tgctgttcgc
      901 ccatgacaac gccacttggg cgccggtcgt gtgacccgtc tcaggagatc gtgatgcggc
      961 cgtcctcgtc gatggggaag gtggggttgt aggcgatctc ccacgggtgc ccgtccgggt
     1021 cggcgaagta gcccgagtag ccgccccagt ccgtgcgctc cgccggcctc atgatgcgcg
     1081 ctcctgcggc caccgccgct tcgaacgcgg catccgcttc cgcctcgctc cccacgttga
     1141 tcgcgagagt gatgccgccg aactccgccg tcggctcgga tcgcagaccc gcatctcggg
     1201 cgaggtcctc ccgtccgaac aggcccagat acgagtccga cgtccggaac cagcagatct
     1261 cgtcgccgac ggagcccgcg agctcccatc cgagcgcttc gtagaacgcc ttggatctcg
     1321 ccacgctgtc gacgccgagc gtcacgatgt tgatccgggc gggaacactc atgtgacctc
     1381 ctttctggca tcgacgctat cgcgcgtctg accggttgct ctccgtagag ttggcccctc
     1441 gatgcccgcc gtgcgactga acccgatgct gcgcgtgatg tggaagctcc atcgcttcgt
     1501 cctccgtgtc tcgggcggtc gcgtcgggtc gcggatgggt tcgatgaagg tgctcctgct
     1561 ggagaccacc ggccacaaga ccggcgagtc ccggacggtc ggcctctccc acctcgagtt
     1621 cgacggccga tacttcgtga tggcgtccta cgccggcgag gaccgcgatc cggcctgggc
     1681 gaagaacctc cgggcgcacc cgcgagcgac ggtgtcgatc ggccgtcgtt ccaccccggt
     1741 ggtcgcgcgg gcgctcgaag gcgccgagcg cgaagcgatg ttcgaccggt tcgtggaggc
     1801 ggacgccgcg taccgcgagt accgagagcg gacgacccgc gacatcccgg tgttcgagct
     1861 gcgcccggaa ggtgcttgag ccgggtgccg tcgatcaccg acatcgaggg cctcaccgtc
     1921 ggtcactgga ccgacgaggt tgcgctcacc ggttgcacgg tggtcttgcc tcctccggga
     1981 acgatcgcct cctgcgaggt tcgaggaggt gctcccggga cgcggggcac ggacatcctg
     2041 cagcctggca cgatcatcga ggtcgcgcac gcgatcgtcc tgaccggcgg gagcgcgttc
     2101 ggtctggcga gcgcgggcgg ggtggagcgc tacttcgaag agcgggggat cggaagcgag
     2161 ataggtccgg cgcgcgtccc gaccgtgccc gccgcggtga tcttcgacct cggtgtcggc
     2221 gatccgtctc gacgaccggg cgcggacgag ggctacgcgg cctgcatcgc cgcatccgcc
     2281 gacgtccgcg aggggcgggt cggggtcggc accggcgcaa cggtcgcgaa gctctgggga
     2341 cccgagcgag cggtgcccgg aggggtcggg acctgggcgg tgcatgacgg cgagctcgtc
     2401 gtcggcgcgc tcgtggtcgt gaacgccgtc ggtgagatcg tggacgagga cggatcgatc
     2461 ctcgtaggtc cgaggctgga gcacggcgag cgtcgcgagg acctggtgga gcagctcgga
     2521 tcgtcgggag cggccaacac gacgctcggc gtggttgcga cgaacgcggt gttgtcgaaa
     2581 gaggacgtcc gccggctggc gagtatcggg aacgacgccc tcgacgtcgc gatccggccc
     2641 gcccacacga tctacgacgg tgacaccgtg ttcgcgttgg ccacgcagca ggtccatgcg
     2701 agcttcgagc aggtcgccgc gttggtcgag cccggcgtca cgaccgcgat ccgcagggcc
     2761 gtcactcgcg acgatggttc aggcgcggcc ggcgcctgag acacgttgct ggtgccagag
     2821 gtgcatcacc gcgtaggcgc gccatgggcg ccagcgttcg gctcgcgcga gcatcgccgg
     2881 ctgcatcgac gccagaccca gggcctcgaa ccccttgcgg acaccgaggt cgcccgccat
     2941 gaacgcatcc gggtcgcgga gcgcgcgcat cgagacgtag gcgagggtcc acggtccgat
     3001 ccctggcacg tcaccgagtc tcctgagcgt ctcctcatgt ggtgcctcgc ccgagagatc
     3061 gagctctccg tccgcgacga gcagggcgac ccggcggatc gtctcggctc gcgcgcgggg
     3121 catcccgaac acctccagcg ggacctcggc cacgcgctct gccgtgggga agagatgggt
     3181 gatccctccg ctcggacgtt cgaggggcgt gccggcggcc gacacgagtc tgccgagcga
     3241 cgtcctcgca cccgccacgg acacctgctg tccgtagatc gcccgcacga cgagctcgaa
     3301 gccgtcgaac gtgcctggga cgcggatccc ggggatccgg gtcaccagcg ggatcatcgt
     3361 cggatctgcg gcgagcgtgg tgtcgatcgt ggcggcgtcg gcgtcgaggt cgaacgctcg
     3421 gcgagcgcgc tcgcggagat cgtcgagatc gggtgagccc tccgacgaga cgcggacgat
     3481 gacgtggtct gagcgaggcg acatcgcggc caccgcggcc ccgctcggct gccgcacgga
     3541 acgcacgtac gtaccatccc gcgtctcctc gatgccgggg acggcacgcg cgccgaggaa
     3601 cgacagcagc gtgtccgctg cgaacggcgc ctggaacggg agccggaacg ctcgtgtgcg
     3661 caactcgttc acgccggcag gtatagcgga tggcctgtcg gtgccaccgg ctaccgtcgc
     3721 acgcctccct ctcctcgaca ggaggagacg atggatcagg tggcgagggt gatcctggcg
     3781 ctcgaggcgc cggaggtaac cgaggaggtc atgcacgtcc tcgatcggag cgggcgtgcg
     3841 cgagtggtgg ccaccgcggg cgacgaccgc cagctcgcgg aggcggtgcg gcagctcgag
     3901 ccggacgtcg tcgtcgccga gcccagcctg ctctccggag cgagagcgtg cggcgtggtg
     3961 ctcgcgatcg acgtccgcga gtcggtgagc tcgctccgga cggcgatccg cgcgggtgcc
     4021 gccggctact tcctgtggcc gaaggagcgc gacgatctgc tcgtggccgc ggcggccgcc
     4081 gtccggcgag cgcagcagac ggccagacga ggcgcggtgg tcgcggtgca cggtggtcga
     4141 ggtggtgcgg gagtcacgtt cgtggccacg cacctcgccg cggcgttcgc ccgccgcggc
     4201 tccgcgatcc tgatcgacgc cgatcccatc ttcggcgacg tcgctcaggc gctgggtgcg
     4261 cccacggggg aggaagcgga accgacccac accttcgccg acgccgccgc cctcggcgag
     4321 gatctcggtc cggagcagct gaggagcgcg ctgtggcggc acgagagcga agtggacgtc
     4381 ctcctgcctc ctccgccgga ggaggcgatc cgcctcggtc cggaggatct gcgtctcgtc
     4441 accgaggtgg ctgcgggtgt cgcggacgtc gtggtcgtgc acctgccgcg cgcgctcggg
     4501 gcgatgacgc gcgccggtgc ggagacctcg gactcgctcg tcgaggtcct cacgctggac
     4561 gtcgtgtcgt tccgagcgac gtcccgcgtc ctggaggcgt gcgcgcccct gcacctcgag
     4621 ggacgcgtga gcttcgtgat caacaaggcg gctcgcagcg agatcacgcc gggagacgta
     4681 catcgtgtct tcggcgaacc cgcccgcgcg atcatcccgc acgaccgcgc cgtccgctcg
     4741 gcgcaggagc gcaaccggct gctgcctccg aaaggcctga tggctcgccg gttcgatcgc
     4801 ctcgcggatg ccctctctgc ccccgtggtc gaagagatgg c
//
LOCUS       DASUVE010000213         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_289325, whole genome shotgun sequence.
ACCESSION   DASUVE010000213 DASUVE010000000
VERSION     DASUVE010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289325"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VF036_04605"
     CDS             complement(<1..249)
                     /locus_tag="VF036_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX6264543.1"
                     /translation="MPTVSVDDVTINEGSGGTVSVVFHVTQNKRGKSAVRYATREGTA
                     KAPGDYVGKSGQIRFAGNKLTKNVSVTVVGDTLDEANET"
     gene            complement(494..1864)
                     /locus_tag="VF036_04610"
     CDS             complement(494..1864)
                     /locus_tag="VF036_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6264544.1"
                     /translation="MKTYRTPTGAVHALRGVTLGFPAGALTAVVGPSGSGKSSLLRVL
                     TGIDRATSGDVWVGPRNVARASARGLRGLRRDVVGYVFQRPSDNFLPNLTIGQHLRLA
                     SRGSAAPEDDGILDRLGIGHRIDHLPDELSGGEQQRAAFAQAVVAGSAVVVADEPTAE
                     LDSESGQLVMDGVRALVDAGVTFVVATHDGAVRELADEVIELEHGRVKGTHPDGPPVA
                     GRSPAARFYGAGRLSDEDFDTAVSVRELSKVFPHHGADVVHALEAVSFEARPGEIVGL
                     VGRSGSGKTTLLNVIAGWEQPTSGEVRWRERIDPRSPSWSTVAAVPQKLGLMEELTVE
                     ENVEYPARLARSLEERRDEIEDLIGVLGIGELRSRYPREASVGEQQRTAIARALAVPT
                     SVLLADEPTAHQDTASASRVFSALRKAADAGTAVVVATHNPEVVRHLDRVLTMADGHL
                     REAPRP"
     gene            complement(1893..>2313)
                     /locus_tag="VF036_04615"
     CDS             complement(1893..>2313)
                     /locus_tag="VF036_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6264545.1"
                     /translation="SIDTFSMLNILGLAAGVLVIGVLVVYLQARQRARTVSNVLSMRM
                     GMRDGQARAALVLELAALLLAAFILGAMTGMIAGRLIAPLLDPLQTIPPPPLFEPPIA
                     AVLWTLGGLAVVSLVGGWLVHRRAAAVDLGEVLRVAE"
BASE COUNT          372 a          830 c          794 g          317 t
ORIGIN      
        1 cgtctcgttc gcctcgtcga gggtatcgcc gaccaccgtc acggatacgt tcttggtcag
       61 tttgttgccg gcgaaacgga tctggccgga cttcccgacg taatcgcctg gcgccttcgc
      121 cgtcccttcc ctcgtcgcat accgcacggc ggacttgccc cgcttgttct gtgtgacatg
      181 gaagacgacg gagaccgtac ccccgctgcc ctcgttgatc gtcacgtcgt cgacggagac
      241 ggtcggcaag ggggcggcgc tcgcttccga gcccgacgag gacgtgtgaa cgaacccgac
      301 gaggaaggcg agggcgaccg ctccgaaggc ggatgcgcgg gatcgagatg agctgatcac
      361 gatcgagtgc tcctctcagc tgacggggat ggttcctctt gctgtaggta aatcggccgc
      421 cctccagcga ccttgagcag ttcgagtgat cccaccatct caacaccgga aatcgggtag
      481 ccgaggagag ggatcagggc cgaggagcct cccggaggtg cccgtcggcc atcgtgagga
      541 cgcgatcgag gtgccggacg acctccgggt tatgcgtggc gaccacgacc gccgtcccgg
      601 catccgccgc cttgcggagc gccgagaaca cgcggctcgc cgacgccgtg tcctgatgcg
      661 ccgtgggctc gtcggcgagc agcacgctcg tcgggacggc cagcgcccgg gcgatcgccg
      721 tccgctgctg ctcgcccacg gaggcctccc gcgggtatcg ggaccggagc tcaccgatcc
      781 cgagcacgcc gatcaagtcc tcgatctcgt cccggcgctc ctcgagcgac cgcgcgagcc
      841 gcgcggggta ctcgacgttc tcctcgacgg tgagctcctc catcaggccg agcttctggg
      901 ggacggccgc gaccgtcgac cacgacgggg accgcgggtc gatgcgctcg cgccaccgga
      961 cctcgcccga cgtcggctgc tcccacccgg cgatcacgtt caggagggtg gtcttcccgg
     1021 acccggaacg cccgaccaga cccacgatct cgccgggtct tgcctcgaag ctcacggcct
     1081 cgagcgcgtg cacgacgtcg gcgccgtgat gcgggaacac cttggagagc tcgcggaccg
     1141 agaccgccgt gtcgaagtcc tcgtccgaga ggcgccctgc cccgtagaag cgcgccgccg
     1201 gcgagcgacc agcgaccgga gggccgtccg ggtgggttcc cttcacgcgg ccgtgctcga
     1261 gctcgatcac ttcatcggcg agctcccgca cggcgccgtc gtgcgtggcg accacgaacg
     1321 tgacccccgc atccaccagc gcgcggacgc cgtccatcac gagctgtccc gactccgagt
     1381 cgagttcggc ggtcggctca tccgcgacga ccacggcgga gccggccacg acggcctgcg
     1441 cgaacgcagc ccgttgctgc tcaccacccg acagctcgtc ggggagatgg tcgatgcgat
     1501 ggccgatgcc caaccggtcg aggatgccgt cgtcctccgg agcggcggag cctcgggacg
     1561 cgagccgcag atgctgcccg atcgtgaggt tcggcaggaa gttgtccgag ggtcgttgga
     1621 acacgtagcc gacgacgtcg cggcggagcc cacggagtcc gcgtgccgac gcccgcgcca
     1681 cgttccgtgg acccacccag acgtcaccgg aggtcgcgcg atcgatcccg gtgaggacgc
     1741 ggaggagcga ggacttcccg ctccccgacg gaccgaccac cgccgtgagc gcgccggccg
     1801 ggaagccgag cgtcacgccg cggagcgcat gcaccgcgcc cgtgggtgtg cgatacgtct
     1861 tcacgagatc gacgcagcgg gccgggacgg gctcactcgg caacgcggag cacctccccc
     1921 aggtcgacgg cggccgcccg ccggtgcacg agccacccgc cgaccaggga cacgacggcc
     1981 aaacccccga gcgtccagag gaccgcagcg atcggcggct cgaacaaggg tggcggcggg
     2041 atcgtctgca gcgggtcgag gagcggtgcg atgaggcgac ccgcgatcat ccccgtcatc
     2101 gccccgagga tgaacgcggc gagcagcagc gcggcgagct cgagcacgag ggcggctcgg
     2161 gcctgcccgt cgcgcatccc catccgcatc gacaggacgt tcgagaccgt cctcgcacgc
     2221 tgacgcgcct gaaggtagac gacgagcacc ccgatcacca ggacacccgc ggcgagcccg
     2281 aggatgttca gcatcgagaa cgtgtcgatc gat
//
LOCUS       DASUVE010000214         3263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_289449, whole genome shotgun sequence.
ACCESSION   DASUVE010000214 DASUVE010000000
VERSION     DASUVE010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289449"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VF036_04620"
     CDS             <1..921
                     /locus_tag="VF036_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6264546.1"
                     /translation="RISRQPGFERFGSDPDEWWRDGETAARLLVRQMREMGGGFPWRL
                     LGDIHAMSEDNVGWAGARTHFDFPRGSVEMRFTCVLHLEHSEWRLVQWHSSLPVSNEE
                     YGFLLTTSVDEIAEAVSELRPDLSASSAADGTVTIAFTDIEDSLRLNALLGDRRWLDV
                     LHAHNDVIRRVTAEHNGTLVKSQGDGSMLAFASARRALTCAIAIDRAIAETFTDPGSP
                     IRVRIGMHVGEPVHEANDFFGHTVNYAARVASSARGGEIVVSSLVHDLLVQTGEFAFA
                     DARRVELKGIDGTQVVYPLAVASSEPASAD"
     gene            complement(941..1153)
                     /locus_tag="VF036_04625"
     CDS             complement(941..1153)
                     /locus_tag="VF036_04625"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6264547.1"
                     /translation="MDHGFAPGDPTRDAIMRVFRDASAAWTLDELARRFGMSIGDAIR
                     VMSDLEAIDFVRRIGDEYVPYRSAAV"
     gene            1345..2565
                     /locus_tag="VF036_04630"
     CDS             1345..2565
                     /locus_tag="VF036_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6264548.1"
                     /translation="MEEILVYGASWCPDCRRAKKFLADQRVPYSWHDIEGDPDGVRVV
                     RDRNDGKNIIPTIVFPDGSHLSEPSNEELAEKLGLQRQAMQHVYDLIVVGGGPTGLTT
                     SIYAARENLETLIVDSKGLGGQAGVTERLDNYPGFPEGIGGAELADRFVKQAERYGVE
                     MLQAISVTLISADSGMTADGDELDVETANGKHYHARAVLLATGSSYRRTGADGEDDLI
                     GAGVHFCATCDGPFYKGSDELMVIGAGNSGLEEGIFLTQFADRVTIVERGDRLRGSRI
                     LQAKVLDHPRMKVELQHGVKEFRKEDGSGKLGSVVVEDLVSGAREEQHPAGVFVFIGL
                     DPNTGFVQGRVDLDERGFVATDPTFMSSIPGVFAAGDVRDGSTKQLASAVGEGAAAAI
                     QVRGYLDLLDRRSM"
     gene            2580..2777
                     /locus_tag="VF036_04635"
     CDS             2580..2777
                     /locus_tag="VF036_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264549.1"
                     /translation="MPMQPTEIFELIVKADERLKYATADKTGTRTQQARDLLTQARDA
                     ARAIGNEQLVQQAELRLSDLA"
     gene            complement(2774..3016)
                     /locus_tag="VF036_04640"
     CDS             complement(2774..3016)
                     /locus_tag="VF036_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6264550.1"
                     /translation="MGRDLTCKELVELVTGYLDGSVRGRRRRRFEAHLAGCDGCTRYL
                     GQMEATIRLTGTLTEEQVTEEQRAVLLAAFRGWSTS"
     gene            complement(3016..>3263)
                     /locus_tag="VF036_04645"
     CDS             complement(3016..>3263)
                     /locus_tag="VF036_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX6264551.1"
                     /translation="WTGIPEDRLLGRETLAVIGRAIDGLPPMQAEVIRLRDALGWTSE
                     EVRNALDLTETNQRVLLHRARAKVRRALEAYLSSGTT"
BASE COUNT          530 a         1059 c         1158 g          516 t
ORIGIN      
        1 aggatctcgc gtcagccggg attcgaacgt ttcgggtccg accccgacga gtggtggcgg
       61 gacggcgaga cggccgctcg cctcctggtg cgccagatgc gcgagatggg tggtgggttc
      121 ccgtggaggc tcctggggga catccacgcg atgagcgagg acaacgtggg ctgggcggga
      181 gcgcgcaccc acttcgattt ccctcgggga tccgtcgaga tgcgtttcac gtgcgtgctc
      241 catctcgagc acagcgagtg gagactcgtg cagtggcata gttcgttgcc ggtgtcgaac
      301 gaggagtacg gcttcctcct cacgacatcg gtcgacgaga tcgcggaggc ggtcagcgag
      361 ctccggccgg acctctcggc ttcgtcggcg gcggacggca ccgtgacgat cgcgttcacc
      421 gatatcgagg actccctccg tctgaatgcg ttgctcggcg accggcgatg gctcgatgtg
      481 ctccacgccc acaatgacgt gatcagacgt gtcacggcgg agcacaacgg cacgctcgtg
      541 aagagccaag gagacggatc catgctggcg ttcgcatcgg cccggcgcgc cctgacatgc
      601 gcgatcgcga tcgaccgcgc gatcgccgag acgttcacgg atccgggctc accgatccgt
      661 gtgcgcatcg gcatgcacgt gggcgagccc gtccatgagg cgaacgactt cttcggtcat
      721 acggtcaact acgcggcccg ggtcgcgtca tcggcgcgag gaggcgagat cgtggtctcg
      781 tcactcgttc acgacttgct cgttcagacg ggggagttcg cattcgccga tgctcgccga
      841 gtggagctga aggggatcga cggcacgcag gtcgtgtatc cgctcgccgt cgcctcgtct
      901 gagcccgcct ccgcagactg agcccagcgc tggcggagac tcagacggcc gcggatcgat
      961 acgggacgta ttcgtcgccg atccggcgca cgaagtcgat cgcctccagg tccgacatca
     1021 cgcggatcgc gtcgccgatc gacatcccga aacggcgtgc cagctcgtcg agcgtccagg
     1081 ccgcggaggc gtcccggaag actcgcatga tcgcgtcgcg cgtagggtcg ccgggtgcga
     1141 agccatggtc catggtcgtc ctcctcggtc gcagtgtctc tcggacgttc ccggacggcc
     1201 aggagttccc cggaaccttc cggacccgtt ccccgatcgt tcacccgatg ccgcgggtcg
     1261 catcgcccgt ggcggcggcg taacggatgc aggcgggcgc gatacggtcc gagcgggagt
     1321 tgtcgaccga cggggagttg agggatggag gagatcctcg tgtacggcgc gagctggtgt
     1381 cccgattgcc gccgcgccaa gaagttcctg gccgaccagc gggtgccgta ctcctggcac
     1441 gacatcgagg gggacccgga cggtgtccgg gtggtccggg atcgcaacga cggcaagaac
     1501 atcatcccga cgatcgtgtt cccggacggg tcgcacctgt cggagccctc gaacgaggag
     1561 ctggcggaga agctcggtct ccagcggcag gcgatgcagc acgtgtacga cctgatcgtc
     1621 gtgggtggcg gacccaccgg tctgaccacg tcgatctacg cggctcgcga gaacctcgag
     1681 acgctgatcg tcgactccaa gggcctcggg gggcaggcgg gggtgacgga gcggctcgac
     1741 aactacccgg ggttcccgga ggggatcggc ggcgccgagc tcgccgaccg gttcgtgaag
     1801 caagcggagc gctacggcgt ggagatgctg caggcgatct ccgtcacgct gatctccgcc
     1861 gactcgggga tgaccgccga cggggacgag ctcgacgtgg agacggcgaa cggcaagcac
     1921 taccacgcac gggcggtcct cctcgccacc ggatcctcct accggcgaac gggcgccgac
     1981 ggggaggacg acctgatcgg cgcgggcgtc cacttctgcg cgacgtgcga cgggccgttc
     2041 tacaagggtt ccgacgagct gatggtgatc ggcgccggca attccggcct cgaggagggg
     2101 atcttcctga cccagttcgc cgaccgggtc acgatcgtgg agcgaggcga ccgattgcgg
     2161 gggtcgagga tcctgcaggc gaaggtcctc gatcacccac ggatgaaggt cgagctgcag
     2221 cacggcgtga aggagttccg gaaggaggac ggctccggga agctcgggtc ggtcgtcgtc
     2281 gaggatctcg tctcgggggc gcgggaagag cagcaccccg caggcgtgtt cgtgttcatc
     2341 gggctcgatc cgaacaccgg gttcgtgcag gggagggtcg acctggacga gcgcgggttc
     2401 gtcgccaccg atccgacgtt catgagctcg ataccgggag tgttcgccgc tggcgacgtg
     2461 cgggatggct cgacgaaaca gctcgcctcc gcggtgggcg agggcgcggc ggcggcgatc
     2521 caggtccgcg gttacctgga cctgctcgac cgccgctcga tgtgagggat gatgccctga
     2581 tgccgatgca gccgaccgag atcttcgagc tgatcgtcaa ggcggacgag aggctcaagt
     2641 acgccaccgc ggacaagacg gggacgagga cgcagcaggc gagggatctg ctcacgcagg
     2701 cccgtgacgc cgcgcgtgcg atcgggaacg agcagctggt ccagcaagcg gagctgcgac
     2761 tctcggatct ggcctagctc gtcgaccaac ctcggaacgc cgccagcagg acggcccgct
     2821 gttcctcggt cacctgctcc tccgtgaggg tgccggtcag ccggatcgtg gcttccatct
     2881 gaccgaggta tcgcgtgcac ccgtcgcacc cggccaggtg cgcctcgaac cggcgccgcc
     2941 gccggccccg cacgctgccg tcgaggtagc cggtcaccag ctccaccagc tccttgcagg
     3001 tcaggtcgcg tcccatcacg tcgtcccgct cgagaggtag gcctccaacg cgcgccgcac
     3061 cttcgcgcgc gcgcgatgca ggagcacccg ctgattggtc tccgtcaggt cgagcgcgtt
     3121 acgaacctcc tccgacgtcc aacccagggc gtcgcgcaat cggatcacct cggcctgcat
     3181 cggagggagc ccgtcgatcg ccctgccgat gacggcgagg gtctcgcgcc cgaggagtcg
     3241 atcctccggg atcccggtcc aat
//
LOCUS       DASUVE010000215         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1231611, whole genome shotgun sequence.
ACCESSION   DASUVE010000215 DASUVE010000000
VERSION     DASUVE010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231611"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..115
                     /locus_tag="VF036_04650"
     CDS             2..115
                     /locus_tag="VF036_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6264552.1"
                     /translation="MSPQTVQTHVRNILGKLRVHSKLEAVAFAVKHGAIAV"
     gene            complement(357..1538)
                     /locus_tag="VF036_04655"
     CDS             complement(357..1538)
                     /locus_tag="VF036_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEX6264553.1"
                     /translation="MARVAGEVVGTDELVVIDLPPGPGWVELVNRLWLEEVPFLPLDH
                     RLTEAERRAIVARARPHFVQDRGGETLLADAVPVAPGVAIVIPTSGTAGEPKLVELHR
                     VAVVVAVSRSAKRLGATPGAPWICPLTPAHIGGLLVLLRGAILGAPVTMHERFEPERL
                     VHDGDGAAFASVVPAMVRRLVASDLGLHGLTLVVGGDGIDDETATRARGRGATIVTTY
                     GLTETCGGFTYDGVPLDGMQVRMDAAGVIEVSGSTLMEGYRLDAAATGAAFTTDGWLR
                     TGDVGAVDDEGHLSIFGRADHVIRTGAEKVWPEEVERALSSHPKVRDVAVAGSPDPEW
                     GSHVRAFVVPARSEDPPSLEELRAHGTEQLARFKLPRELQLVEEIPRTSTGKVRRGAL
                     P"
     gene            1628..>2097
                     /locus_tag="VF036_04660"
     CDS             1628..>2097
                     /locus_tag="VF036_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6264554.1"
                     /translation="MDWAPGGARLKDPPESHPETTFTDIRYETAEGIAKITMARPEVR
                     NAFRPLTTRELIEAFDLARDDASVGVVILTGEGSLAFSSGGDQGVRGDDGYIDEHGVG
                     RLNVLDLQIQIRRLPKPVIAMVAGYAIGGGHVLHLCCDLTIAADNARFGQTGPRV"
BASE COUNT          326 a          780 c          707 g          284 t
ORIGIN      
        1 catcagcccc caaacggtgc agacgcacgt ccggaacatc ctcggcaagc tccgagtgca
       61 ctccaagctc gaggcggtcg ccttcgccgt caagcacggc gcgatcgccg tctagcccgc
      121 tcgaggtcgg aaactcctga ccggcagggc tcaagtcacg ttccgggcag gacgaagcca
      181 ctaccggaga gcgacccgcg ccctcgggcg cggagaggcc gtcaccccct cgatggcctc
      241 ggggacctcc ccgagcgaag gcccgagtcc acccctcccg gactcgggcc tttcatctgt
      301 ttggcgccct tgtcgccatg gcgctgatgc cgagggcgcc gaggccgagg gcgcgatcac
      361 gggagcgcgc cgcggcgcac tttcccggtc gatgtccgag ggatctcctc caccagctgc
      421 agctcgcggg ggagtttgaa ccgcgccagc tgctcggtgc cgtgggcgcg cagctcctcg
      481 agcgacggcg gatcctccga tcgagccggc acgacgaacg cccgcacgtg cgatccccat
      541 tcgggatcgg ggctgccggc gacggccacg tcgcgcacct tcgggtggga cgacagcgcg
      601 cgctcgacct cctcgggcca gaccttctcc gcgccggtcc ggatcacgtg gtccgccctc
      661 ccgaagatcg agaggtgccc ctcgtcgtcg accgcaccga cgtcgcccgt gcggagccat
      721 ccgtcggtgg tgaacgcagc gcccgtcgcg gccgcgtcca gtcggtatcc ctccatcaac
      781 gtggaccccg agacctcgat cacgccggcg gcgtccatgc ggacctgcat gccgtccagc
      841 ggcacgccgt cgtacgtgaa ccctccgcac gtctccgtca ggccgtacgt cgtgacgatg
      901 gtggcgccgc ggccgcgcgc tcgggttgcc gtctcgtcgt cgataccatc gccccccacg
      961 acgagcgtca ggccgtgcaa tccgaggtcc gacgccacga ggcggcggac catcgccggg
     1021 acgaccgacg cgaacgcggc gccgtcgccg tcgtggacga gccgctcggg ctcgaaccgc
     1081 tcgtgcatcg tcaccggagc accgaggatg gcgccgcgga ggagcaccag caggcccccg
     1141 atgtgcgcgg gggtgagcgg gcagatccac ggggcacccg gggtggcgcc cagccgcttc
     1201 gccgaccggc tgacggcgac gacgacggcc acgcggtgga gctccaccag cttcggctcc
     1261 cccgccgtcc ccgaggtcgg gatcacgatc gcgacacccg gcgcgaccgg cacggcgtcg
     1321 gccaggaggg tctcgccacc acggtcctgg acgaagtggg gacgagcgcg agccacgatc
     1381 gctcgccgct ccgcctcggt gagccggtga tcgagcggga ggaacggcac ctcctcgagc
     1441 cagagtcggt tgacgagctc gacccatccg ggaccgggcg gcaggtcgat cacgaccagc
     1501 tcgtctgttc ccacgacctc ccccgccact cgggccaccg acgcgtgggg taggctaccc
     1561 aaccgttcga gcgcgcgctc taggagtcaa cggtcatatg gacgagtcac gtgagatccg
     1621 acaggccttg gattgggccc cgggtggagc tcggctgaag gatccgccag agagccaccc
     1681 ggaaacgacc ttcaccgaca tccgatacga gacggccgag gggatcgcga agatcacgat
     1741 ggcgcggccc gaggtccgca acgcgttccg accactcacc acgagggagc tgatcgaggc
     1801 cttcgacctc gcccgggacg acgcttccgt cggggtggtg atcctgaccg gcgagggctc
     1861 cctggcgttc tcctccggtg gcgaccaagg cgtccgcggc gacgacggct acatcgacga
     1921 gcatggcgtc gggcgtctca acgtgctcga tctccagatc cagatccgtc gcctgcccaa
     1981 acccgtgatc gccatggtcg ccggctacgc gatcggaggg ggccacgtcc tccacctctg
     2041 ctgcgacctc acgatcgccg cggacaacgc ccgcttcggg cagacggggc cgcgcgt
//
LOCUS       DASUVE010000216         3283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_290498, whole genome shotgun sequence.
ACCESSION   DASUVE010000216 DASUVE010000000
VERSION     DASUVE010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290498"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VF036_04665"
     CDS             complement(<1..682)
                     /locus_tag="VF036_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264555.1"
                     /translation="MSSARRLWLVARREWNQRVRTLAFRISTLISILIVGALILVPDM
                     YGGGADAPTVGLVGESSPELPDLVRVSGDQLGLSVEIHEFREGSAGRAALRSDDVSVL
                     LVDQQQLVWKAETDDQLEAAVTSAVQVLQQREAIAELRLTPQEARRLLGAPDLPSTSL
                     EPFTQQRSEREELAWVGLVVLFTAIAFYGGFLLVGVVEEKSSRTVEVLLARMPARSLL
                     AGKIAGIGL"
     gene            complement(679..1506)
                     /locus_tag="VF036_04670"
     CDS             complement(679..1506)
                     /locus_tag="VF036_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6264556.1"
                     /translation="MAPGRIVGLLGPNGAGKTTMMRAIFGLVRPDRGEVLWKRKPVGL
                     QERARFGYMPEERGLYPKMKVGEELTYFAELSGMSGTAAKEAAARWLERLGLGDRADA
                     RLEALSHGNQQRVQLAAALAHDPELAVLDEPFSGLDPLGVESLGELLVQTAATGVAVV
                     FSSHQLDLVEDVCKDVVIIDRGKVVLAGVVEELKAASPRRSLEITVDGHPWVPVVPSS
                     TVTSKRDGRLRVLVDSSVDVAEVLASAHRVGEVTTFSFEPPSLMDLFLAAVSEGEGS"
     gene            1802..2134
                     /locus_tag="VF036_04675"
     CDS             1802..2134
                     /locus_tag="VF036_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264557.1"
                     /translation="MNKRSAVLIAAGLVLTLAIGGLAVSLGLTGPTPVNAAGPARPER
                     VVKVERRTVTIHQKAGSPSAATVMLSAPASDPAIDGDSDDGYDEDASEHEDESHEESE
                     EVESEDVG"
     gene            2131..2430
                     /locus_tag="VF036_04680"
     CDS             2131..2430
                     /locus_tag="VF036_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264558.1"
                     /translation="MTGAPRRVASKGALRLAAWSSAAAALVASWGVFGALPKPIAAAT
                     VLQTRPQKIVVVRQVLRRVIVQEAAPATPPTTVTFTPASGSSAPPAPTSTGGSAP"
     gene            2451..>3283
                     /locus_tag="VF036_04685"
     CDS             2451..>3283
                     /locus_tag="VF036_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX6264559.1"
                     /translation="MGTSVRVLTEGGSGVPVGPAVKTIARTFEREERRFSRFRGDSEL
                     SLVNAAEGRSVRISASFRSVIRLALDAAASTGGLFDPTVLGAMGAVGYDRTFDEVLAG
                     ARGTLHPTIPCGRWRDVVLEDDVLSLPLGVGLDLGGLVKGWTADQAATRAVDAGLPWV
                     LVNAGGDLRIAGDAPRIDIAVEDPDDPSSEVGRLSVREGGVATSSTRSRSWGDGLHHV
                     IDPRTGSPSATDLVQVTVTARTCAEAEILATAVLLRGSGAAGEASVSVTSGGRVLVTA
                     P"
BASE COUNT          496 a         1142 c         1117 g          528 t
ORIGIN      
        1 gcaggccgat gccggcgatc ttgccggcca gcaggctccg ggccggcatg cgcgcgagca
       61 ggacctcgac ggtgcggctg gacttctcct ccacgacgcc gacgagcagg aaccccccgt
      121 agaaggcgat ggccgtgaac aagacgacga gaccgaccca ggcaagctcc tcccgctccg
      181 agcgctgttg cgtgaagggc tcgaggcttg tcgacggcag gtccggtgcc ccgagcagcc
      241 gtcgtgcctc ctggggagtg aggcggagct cggcgatcgc ctcccgttgt tggaggacct
      301 ggaccgccga ggtcacggcg gcttcgagct ggtcgtcggt ctccgccttc cacaccagct
      361 gctgttgatc cacgagcaac accgacacgt cgtccgaccg gagagcagct cggcccgccg
      421 atccctccct gaactcgtgg atctccacgg agagacccag ctggtcaccg gagacgcgga
      481 cgaggtcggg gagctccggc gagctctcgc ccacgaggcc gacggtgggc gcgtctgcgc
      541 ctccgccgta catgtccgga accaggatca gagcccccac gatcaggatc gagatgagcg
      601 tcgagatccg gaacgcgagc gtgcgtaccc gctggttcca ctcgcggcgg gccaccagcc
      661 acagacgccg tgcgctgctc atgacccttc tccttccgac accgccgcga ggaacaggtc
      721 catgagcgag gggggctcga agctgaacgt cgtgacctcc ccgacccggt gcgcgctcgc
      781 caggacctcg gccacatcga cgctggaatc cacgaggacc cgcagccggc cgtcgcgctt
      841 gctcgtaacg gtgctgctcg gcacgaccgg cacccacgga tgcccgtcta cggtgatctc
      901 caatgagcga cggggagagg cggccttcag ctcctccacc accccggcca acacgacctt
      961 accgcggtcg atgatcacga cgtctttgca gacatcttcg accagatcca actggtggct
     1021 cgagaacacc acggccacgc cggtggcggc ggtttgcacg agcagctcgc ccagggactc
     1081 caccccgagc gggtccagcc cgctgaacgg ctcgtcgagc acggcgagct cgggatcgtg
     1141 cgccagggcc gccgccagct gcacccgctg ctggttgccg tgggagagcg cttcgagccg
     1201 cgcgtccgct ctatcgccga gacccagccg ttcgagccat cgagcggccg cttccttcgc
     1261 agccgtgccg ctcatccccg acagttcggc gaagtaggtg agctcctccc cgaccttcat
     1321 cttcggatag aggcctcgtt cctccggcat gtagccgaac cgagcccgtt cctgaaggcc
     1381 gaccggcttc ctcttccaca ggacctcgcc ccggtctggg cgcaccaacc cgaagatcgc
     1441 ccgcatcatg gtggtcttcc cggccccgtt ggggccgagc aaccccacga ttcgccccgg
     1501 tgccacgctg aaggatgcgc cgtccagagc gaccactctc cgtagcgctt cgtgaggtcg
     1561 tggatctcga gcatctgtcc tccgtcgcat cctttctatc gcgggccagg gtcgatcccg
     1621 gggggaggat gtcccagtgg atgcgggacc ttcggctacc tctgtccctg tcggatcggc
     1681 tcgtccgatc tggcgagatg acggcccctc ggatccttgg tgagccctta accctcggtt
     1741 ggaagctgct tgcatccgcc gttcgtagcg tggcggcacg ctcacgaagg agggggaagc
     1801 gatgaacaag cgatcggcag tcctcatcgc cgctggactc gtgctcacgc tggccatcgg
     1861 aggactcgcg gtgtcgctcg gcttgacggg tccgacgccg gtcaacgcgg ccgggcctgc
     1921 tcggcccgag cgagtggtga aggtggagcg gagaaccgtg accatccacc agaaagcggg
     1981 ttcgccctcc gcagcgaccg tcatgctctc ggcccccgcg tccgaccccg ccatcgacgg
     2041 cgactcggac gatggatacg acgaggacgc gagcgagcac gaggacgagt cccacgagga
     2101 atccgaggaa gtggagtcgg aggacgtcgg atgaccggtg caccccgccg cgtcgcgagc
     2161 aagggagccc tgcggctggc ggcatggtcg tcggccgcag ccgccctcgt cgcgtcctgg
     2221 ggagtgttcg gcgcgttgcc caagccgatc gctgctgcaa ccgtgttgca gacccggccg
     2281 cagaagatcg tggtcgtgag gcaggtcctg cgccgggtga tcgtgcagga ggccgccccg
     2341 gcgaccccgc ccaccaccgt gacgttcacg cccgcatccg gttcgtccgc tccacccgcc
     2401 cccacgtcca ccggggggtc agcgccgtga ggacgtggag cttccgcgcc atgggaacgt
     2461 cggtgcgcgt cctgaccgag ggtggctccg gcgttccggt cgggccggcg gtcaagacca
     2521 tcgctcgcac cttcgagcga gaggagcggc gcttctctcg tttccgaggc gacagcgagc
     2581 tttctctcgt caacgctgcg gaagggcggt cggttcggat ctcggcctcc ttccgttcgg
     2641 tgatccgtct tgccctcgac gccgcggcgt cgacaggagg actcttcgat ccgacggtgc
     2701 tcggtgccat gggagcggtc ggctacgacc gcacgttcga tgaggtgctg gccggagcgc
     2761 ggggaaccct gcatccgacc attccgtgtg ggcgctggcg cgacgtggtc ctcgaggacg
     2821 atgtcctttc actcccgctc ggcgtcggcc tggatctcgg aggcttggtg aaggggtgga
     2881 ccgcggacca agcggcgact cgggccgtcg atgcggggct tccgtgggtg ctcgtcaacg
     2941 cgggtggcga tctgaggatc gcgggcgacg cccctcggat cgacatcgcc gtcgaggatc
     3001 ctgacgatcc ctcgagcgag gtggggcgtc tgtcggtacg cgagggcggg gtcgcgacat
     3061 cgtcgacccg ctcgcggtcg tggggtgacg gtctccacca tgtgatcgac ccgaggaccg
     3121 gctcgccgtc agccaccgac ctggtccagg tgacggtcac cgcgcggacc tgtgcggagg
     3181 cggagatcct ggccacggct gttctgctgc ggggttccgg tgccgccgga gaagcatccg
     3241 tgagcgtcac gtccggaggg cgcgtgctcg tcacggctcc gat
//
LOCUS       DASUVE010000217         3107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1421891, whole genome shotgun sequence.
ACCESSION   DASUVE010000217 DASUVE010000000
VERSION     DASUVE010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421891"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..140)
                     /locus_tag="VF036_04690"
                     /pseudo
     CDS             complement(24..140)
                     /locus_tag="VF036_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234777.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            649..927
                     /locus_tag="VF036_04695"
     CDS             649..927
                     /locus_tag="VF036_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264560.1"
                     /translation="MDDDELLAQAEEALTEIKVAHGLSERHASVLAALRNRLKGTAGR
                     SLDEMLEAADDPERDRLEQELAKAENERRPSLDDLLAEDPKPRPDWPS"
     gene            1086..2051
                     /locus_tag="VF036_04700"
     CDS             1086..2051
                     /locus_tag="VF036_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX6264561.1"
                     /translation="MKAAVRTRYGPPENVRIADVPTPAPGHGEVLVRVHATTVNRTDC
                     GFRAASPWIVRFFAGLAKPRVTILGSEFAGEIASVGPGVSAFEAGDRVFGFSEDRFGA
                     HAEFLTMPADGAIVSMPADLTFEETAPSTEGSHYALSFIRKARIRGGRRVLVNGATGA
                     IGSAAVQILTDLGATVTAVCATEHVELVRGLGPDRVIDYRTEDFTKDAERYHVVFDAV
                     GKSSFGRCKRLLLPGGVYLSSDLGPLSQNPLLALVTPLFGGRKVLFPIPRPHGRREMI
                     YVKQLIESGAFRPLIDRRYPLDEIVDAYRYVETGQKVGNVVIEMP"
     gene            complement(2066..2620)
                     /locus_tag="VF036_04705"
     CDS             complement(2066..2620)
                     /locus_tag="VF036_04705"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6264562.1"
                     /translation="MTATPPAPGEPPLSHQIGDLAEVLSNLGVFRVMPEDVLALCPSG
                     FRRVPINQGSEHYLSLQGFAPDQIAYAHTHPDSEEWVVALAGSGKAFLSDVPVPLEPG
                     VVIGRAAANPHGFHSGNAPLHLLSLQIPRPSEATTTWDQPGETTEPAHCASGGTCRRC
                     PRCGGHSANVRARTFLCENCSFEF"
     gene            complement(2684..>3107)
                     /locus_tag="VF036_04710"
     CDS             complement(2684..>3107)
                     /locus_tag="VF036_04710"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6264563.1"
                     /translation="TAEGEIQIAAPPETVWAVLTELRAWPTWNSDVQSMAFDGPLAPG
                     STFRWKAGSTSLTSTLRVVEAPREVGWTGTTMGIHAIHVFYLEPSDGGTRARSAESFR
                     GLIPSVLKKYSRRVLQRGIESILASLKLEAERRAATRS"
BASE COUNT          480 a         1091 c         1059 g          477 t
ORIGIN      
        1 ctctctgcaa cgcccatgca gactcaggcc gctgtagctt gtgacgcgat cagcctgtcc
       61 gccgtggata gcgcctgggc tacgacctgg tccatgttca ggtactggta gcgcgcgagc
      121 cgtccgacga acgtgacgtc ccagtagtcc caccgtccct cgaggtcgtc gatgctgccc
      181 ccgatcgtgg ccccgtcctc gacctcggga gcctggaggc tgacgacgtc gcctcccacc
      241 cgcgagcccg agcgcaggat gacgtccccg ttgaacacga tgacgtcctc cccgaccgtc
      301 ccgatgatct cggtgcgtcc gttgaacacg acgagcgtct gctcgaccgt cccctcgatc
      361 gtggcttcgc cgttgaagat caccgcgtgc tggaccgtct cgccctcggg cacgacgagg
      421 cgcccggtga tcatgatctg gtcgtcggtg tcgggtccgc cgtcgtcctg agcggtggcc
      481 gcgggagcga gccccacgcc gatggcgacg agcgtcgaga acacgaccgt tgccactgtc
      541 cgcggcgagc gccccctcat cctccttact cttcaccctc cgttcgaacc acgcaactcg
      601 gacgcgcctg ggtcgcgagg cgctcgcgag gggtggcaga ctgtctccgt ggacgacgac
      661 gagctcctcg cgcaggcgga ggaagccctg accgagatca aggtcgccca cggcctgtcc
      721 gaacgtcatg cctcggtgct ggcggcgttg cgcaaccggc tgaagggcac cgccgggagg
      781 tcgctggacg agatgctcga ggctgcggac gaccccgagc gggaccggct cgagcaggaa
      841 ctggcgaagg ccgagaacga acgcaggccg tccctggacg acctcctcgc ggaagatccc
      901 aagccgaggc cggactggcc gtcctgatcg gcgggcacgt gacgagcaga tgaggatcgg
      961 ctcggaggcc tggtcaccga cctggatcgg cgcgctcctt cgggggcatc gcttcgaggg
     1021 tccccggtgg caccggaact ctcaccgaag ctcgggctct ggctccgagc cgtgagcgac
     1081 gatcgttgaa ggccgccgtt cgcacccggt acggcccacc ggagaacgtt cggatcgcgg
     1141 acgtccccac cccggccccc gggcacggcg aggtgctcgt gcgggtccac gcgacgaccg
     1201 tcaaccggac ggactgcggc ttccgcgccg ccagcccgtg gatcgtccgg ttcttcgcag
     1261 gcctcgcgaa gccgagggtg acgatcctcg ggagcgagtt cgccggggag atcgcgtcgg
     1321 tcggaccggg cgtctccgcc ttcgaggccg gagaccgcgt gttcggcttc agcgaggacc
     1381 ggttcggcgc ccacgccgag ttcctcacga tgccggcgga cggggcgatc gtgtcgatgc
     1441 cggccgacct gaccttcgag gagaccgcgc cgagcaccga gggctcccac tacgcgctct
     1501 cgttcatcag gaaggcgagg atccggggcg gccggcgcgt gctcgtgaac ggcgcgaccg
     1561 gggcgatcgg ctcggcagcg gtgcagatcc tcacggatct cggcgcgacc gtgacggccg
     1621 tctgcgccac cgagcacgtc gagctcgtgc gcgggctcgg tcccgaccgg gtcatcgact
     1681 atcggaccga ggacttcacg aaggacgccg agcgctatca cgtcgtcttc gacgccgtgg
     1741 gcaagagctc gttcggacga tgcaagcgac tgctgctgcc gggcggggtc tacctctcat
     1801 cggacctcgg gccgctctcg cagaacccgc tcctcgcgct cgtcacgccg ctgttcggag
     1861 gacggaaggt cctcttcccg atcccgcgcc cgcacggccg cagggagatg atctacgtga
     1921 agcagctgat cgagtcgggg gcgttccggc cactgatcga ccggcgatac cccctggacg
     1981 agatcgtcga tgcctaccgg tacgtcgaga ccgggcagaa ggtgggcaac gtcgtgatcg
     2041 agatgcccta ggaccgtcgc cccggtcaga actcgaagct gcagttctcg cagaggaacg
     2101 tccgggcgcg gacgttcgcg ctgtgacccc cacagcgggg acagcggcgg caggtccctc
     2161 cgctcgcgca gtgggccggc tcggtcgtct cgcccggctg atcccacgtc gtcgtcgcct
     2221 cggacggcct agggatttgc agcgagagca ggtggagcgg agcgttcccg ctgtggaagc
     2281 cgtgcgggtt cgccgccgcg cgtccgatca ccacgcccgg ctcgagcggc accggcacgt
     2341 ccgacaggaa cgccttgccg gaaccggcga gcgccacgac ccactcctcc gagtctggat
     2401 gcgtgtgcgc gtacgcgatc tggtccggcg cgaacccctg cagggacagg tagtgctcgc
     2461 tcccctggtt gatcgggacc cgccggaatc ccgacgggca aagggcgagg acgtcctccg
     2521 gcatcacccg gaacacgccg aggttggaca gcacctccgc gaggtcgccg atctgatgcg
     2581 acaagggtgg ctcgcccggc gccggtgggg tggcggtcat gacacggatc ttccgacccg
     2641 tcccgggtcg cccgcaaacc gccggtgccc gagatcgcga ccctcagctg cgtgtcgcag
     2701 cacgacgctc ggcttcgagc ttcaacgagg cgaggatgct ttcgatgccc cgctgaagaa
     2761 ccctgcggct gtacttcttg aggacggacg ggatgagacc tcggaacgac tccgcggatc
     2821 tcgctcgcgt gccgccatcc gatggctcca ggtagaacac atggatcgcg tggatcccca
     2881 tcgtcgtccc cgtccagccg acctcgcggg gagcctcgac cacccggagc gtcgatgtga
     2941 gggacgtgga acccgccttc cagcggaacg tcgaacctgg tgcaagcggg ccgtcgaacg
     3001 ccatcgactg gacgtccgag ttccacgtgg gccaggccct gagctccgtc aagaccgccc
     3061 agacggtctc cggtggcgcc gcgatctgga tctcaccctc cgcggtg
//
LOCUS       DASUVE010000218         5788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_856288, whole genome shotgun sequence.
ACCESSION   DASUVE010000218 DASUVE010000000
VERSION     DASUVE010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856288"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1440
                     /locus_tag="VF036_04715"
     CDS             <1..1440
                     /locus_tag="VF036_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX6264564.1"
                     /translation="LLIAFLLSRIEDEQHRGGPLRNDRPPEGAAPINTAFEGLVTFRG
                     NATRSYYGEGPVPLDPVIGWRAPSARLCSMSFVGIERSEWCGTGWTGQPNVIVSEDGS
                     IEVREGAYDGMYHFLDGLTGLPLRQPLATRDLAKGSATSDPDGFPLYYVGSRDGRFRI
                     VATDRHARGEPLVLWSIDGATSVERPLRNDDWDGAALVVDDVLFEGGENGWLYVIRLH
                     RSYDELGLVAVDPEVVATVPGFDDELLRALGDTEVSIENSVSLHEGILYFANSGGLVQ
                     GWDVSDVLAGGSDLERVFRFWTGDDTDATVVIDDAGDLYVASEYQRSGERSSEVGQLM
                     KLDPSRPSDPLVWSIDAREIGFEGAGGSWSTPALYGDLVYFTTAAGRVLAVERDTGDI
                     RWERQIGAPAIGSPVVVDGVLVQGDCAGDLWAWDVSEPEADPVLLWKLHFSDCIESTP
                     AVWRGWIYLGTRQGYLYGLTERGSVDTAP"
     gene            complement(1451..2404)
                     /locus_tag="VF036_04720"
     CDS             complement(1451..2404)
                     /locus_tag="VF036_04720"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX6264565.1"
                     /translation="MEQTPTMPPPPIRERCYRHPDVETGVHCTRCGRPICPDCMIPAP
                     VGHQCPECVAEARREYRRGPGRQIAVANVKSTSVTKLLLIAIAIGYIWELVIAGGPGS
                     LFEGPSGEALVDAGALVPFTGTNGAQLVGGLVGGEWWRLLSSIFLHAGVIHLALNAYV
                     LWIFGTAIERDIGRVSTLAVFLVTGVFAGATSFAFASRFSVAVGASGAIFGLVGAFVA
                     YNYLRRSHVMAQARLRAALSMLLINLLIGFSVPVIDWRAHVGGLVAGLVAGFAVDPSR
                     PAAIRRFAAAGGLVALLVVAAALVVYRSAQITADPSILFPD"
     gene            complement(2448..3419)
                     /locus_tag="VF036_04725"
     CDS             complement(2448..3419)
                     /locus_tag="VF036_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264566.1"
                     /translation="MTVRRISLATLALLLGAMSCTAEPTGGSDVDTTFAAQMASTWHY
                     TGAPQRVQVGIAASDTDGVRVVTQGTVDLAFAYLGTGSGAPEAGPTATGKYVPVPGTD
                     ASGEAPTIASGARGVYEAEGVTFDRAGTWRATLSPEIDGVARQLTVDFPVTELSDVHI
                     PAPGEPALHTDTLTMDSKGRKGAIDSMADGGGKIPDPELHRWSIADAIDEGRPALVLF
                     GTPAYCASQFCGPEVTELQRFATEHPDRAVYIHVEIWKDFNAQPQVMNEGAADWLLRT
                     MPDGSQNMTEPWLYLIGADGVIVDRWGSLFDPADVEAALEALPPMNA"
     gene            complement(3433..3948)
                     /locus_tag="VF036_04730"
     CDS             complement(3433..3948)
                     /locus_tag="VF036_04730"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX6264567.1"
                     /translation="MADEGWTLWRTTAETPLGPFTILLSDAGVVRTHFDEDGAPDDDL
                     DAVEDRRRTAPIRREIDAYFRGRLRAFETPADLSEMGEGFTRRVLETVRTIPYGELWT
                     YGDVAAAAGATRAGRAAGSALARSPIELFVPCHRVVRAGPSLGSYGRHDDRRRFLLLL
                     EGALSGSSSRP"
     gene            complement(3954..4697)
                     /locus_tag="VF036_04735"
     CDS             complement(3954..4697)
                     /locus_tag="VF036_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HEX6264568.1"
                     /translation="MSRDERALEAGLSDPAVPRVLGTGELEVLGLLPNASNHTFLARV
                     KSTEEELLAVYKPRRGEAPLWDFPEGTLCAREVAAYVVSRALGWPNVPATVLRDGPYG
                     VGSVQRFIPHDPEEHYFTLVERFPEDFRRVAAFDMVVNNADRKSGHCLLGEDGRIFLV
                     DHGVCFSDEPKLRTVIWDFIGDPVEEETRADVRRLAGEIRGGEVRDELANLLAPAELA
                     ALADRADTVAAAVRFPEPGPDRPFPWPPI"
     gene            complement(4661..5236)
                     /locus_tag="VF036_04740"
     CDS             complement(4661..5236)
                     /locus_tag="VF036_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HEX6264569.1"
                     /translation="MDLGPVDRITADAIGEPGARVFYIQARAGVELVTVIVEKQQVQL
                     LAASVLELLEDVPSAELVTELGAGSAGTPALEGSAMALEEPIDPRWRAGRLSIGFDQD
                     RDLFLLEVEEFRPDPEDLEEDDPRSVLGAEVGAKPESIRIWASPTQMLALSRHGAEVA
                     ARGRPTCQFCGNPIDAEGHACPAMNGHSKRD"
     gene            complement(5241..>5788)
                     /locus_tag="VF036_04745"
     CDS             complement(5241..>5788)
                     /locus_tag="VF036_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6264570.1"
                     /translation="FEGVRLTAIYSSPLERCIETLEPLATARRLEMRTSEALIEMDAG
                     NWTGRTLPSLRRTKLWGTVQRSPSRFQFPSGESFVDAEARLLDQIERIVARHPRGRVV
                     VGTHGDLVGMLVSHYAGAHLDQFQRVVVDPASVSVVHIGDGVPRILLVNDTGSLDRFT
                     AAPPKAANAAARDGRGRKLRG"
BASE COUNT          885 a         1960 c         2078 g          865 t
ORIGIN      
        1 ctgttgatcg cgttcctcct gtcccggatc gaggacgagc agcaccgcgg tggcccgctc
       61 aggaacgatc ggccgccgga gggcgcagcc cccatcaaca cggcgttcga gggcctggtc
      121 acgttccgag gcaacgcaac acgcagctac tacggcgagg gacccgtgcc gctggatccg
      181 gtgatcgggt ggcgcgctcc ctcggcccgg ctctgctcga tgtcgttcgt gggcatcgag
      241 cggagcgagt ggtgcgggac cggctggacg ggacagccga acgtcatcgt gagcgaagac
      301 ggctcgatcg aggtccgcga gggcgcctac gacggcatgt accacttcct ggacggtctg
      361 accggtctgc cactgcggca gccgctcgcg acccgcgacc tcgcgaaggg ctcggcgacg
      421 tcggacccgg acgggttccc gctctactac gtgggctccc gcgacggccg cttccggatc
      481 gtggcgacgg atcgtcacgc tcgcggcgag ccgctcgtgc tctggtcgat cgacggggcc
      541 acgagtgtcg agcggccgct ccgcaacgac gactgggacg gcgccgcgct ggtcgtggac
      601 gacgtcttgt tcgagggcgg ggagaacggg tggctctacg tgatccgttt gcaccgcagc
      661 tacgacgagc tcgggctggt cgcggtcgat cccgaggtcg tggcgaccgt cccggggttc
      721 gacgacgagc tcctccgagc actcggcgac accgaggtct cgatcgagaa ctcggtctcc
      781 ctccacgagg ggatcctgta cttcgcgaac tcgggcggcc tggtgcaggg gtgggacgtc
      841 tccgacgtgc tcgccggcgg ctccgatctc gagcgggtct tccggttctg gaccggggac
      901 gacaccgatg ccacggtcgt gatcgacgac gccggcgatc tctacgtggc cagcgaatat
      961 cagcgatccg gggaacggag cagcgaggta ggccagctga tgaagctcga cccgtcgcgt
     1021 ccgagcgacc ccctcgtgtg gtcgatcgat gcccgggaga tcggcttcga gggtgccggg
     1081 ggcagctggt cgacgcctgc cctctatggg gatctcgtgt acttcacgac ggccgccggt
     1141 cgcgtgctgg ccgtggagcg ggacaccggc gacatccgct gggagcgaca gatcggcgcc
     1201 ccggcgatcg gatcgccggt cgtcgtggac ggcgtgctgg tccagggcga ttgcgcgggg
     1261 gatctgtggg catgggacgt gtcggaaccc gaagcggacc cggtgctgct ctggaagctg
     1321 cacttcagcg actgcatcga atcgacgccg gcggtgtggc gcggctggat ctacctcggc
     1381 acgcggcagg gctacctcta cgggctcacg gagcggggga gcgtcgacac cgcaccttga
     1441 gaacgtcggg tcaatccggg aacaggatcg acgggtccgc cgtgatctgc gcgctccggt
     1501 agacgacgag cgcggctgcg accacgagca gcgccacgag gccgccggcg gcggcgaacc
     1561 gacggatcgc cgccggccgg gacggatcga ccgcgaagcc cgcgacgagg ccggcgacga
     1621 gtcctccgac gtgggctcgc cagtcgatca cggggaccga gaagccgatc aacaggttga
     1681 tgaggagcat cgagagggct gcccggagcc tcgcctgcgc catcacgtga ctgcggcgga
     1741 gatagttgta cgcgacgaat gctcccacca gcccgaagat cgcgcccgac gcgccgaccg
     1801 cgacactgaa cctggaggcg aacgcgaagg acgtcgcgcc cgcgaagacg cccgtgacga
     1861 ggaacaccgc gagcgtggag acacggccga tgtcccgctc gatcgccgtg ccgaagatcc
     1921 acaggacgta cgcgttcagc gccaggtgga tcacgcctgc gtggaggaag atcgagctga
     1981 ggagccgcca ccactcgcct cccaccagcc cgccgacgag ctgcgcgccg ttcgtcccgg
     2041 tgaagggcac gagggctcct gcatcgacca gtgcctcgcc ggagggtccc tcgaacaggg
     2101 agcctggtcc gcccgcgatg acgagctccc agatgtaccc gatcgcgatc gcgatcagca
     2161 ggagcttcgt gaccgacgtc gacttcacgt tcgccaccgc gatctggcga ccgggacccc
     2221 gccggtactc gcgtcgagcc tcggccacgc actcgggaca ctgatggccg accggggcgg
     2281 ggatcatgca gtcggggcag atgggtcggc cacaccgcgt gcaatgcacg ccggtctcaa
     2341 cgtcggggtg ccggtagcac cgttccctga tgggcggcgg gggcatcgtg ggtgtctgtt
     2401 ccacgggcca atcgtacgtg ggaggtgatg gaacgagggc tagagggcta ggcattcatc
     2461 ggcgggagcg cctcgagtgc cgcttccacg tctgcgggat cgaacagtga cccccaccga
     2521 tcgacgatca ccccgtcggc gccgatcagg tagagccatg gctcggtcat gttctgcgaa
     2581 ccgtcgggca tggtgcggag gagccagtcg gcggcgccct cgttcatgac ctggggttgt
     2641 gcgttgaagt ctttccagat ctcgacatgg atgtagacgg ctcgatcggg gtgctcggtg
     2701 gcgaaccgct gcagttccgt cacctcggga ccgcagaact ggctcgcgca gtacgcgggc
     2761 gtcccgaaca gcacgagcgc gggccgccct tcgtcgatcg cgtcggcgat cgaccatctg
     2821 tggagctcag ggtcggggat cttgccccct ccgtccgcca tcgagtcgat cgcgcccttc
     2881 cgccccttcg agtccatcgt gagcgtgtcg gtgtggaggg ctggctcgcc gggggcgggg
     2941 atgtggacgt cggagagctc cgtcaccggg aagtccaccg tcagctggcg ggcgaccccg
     3001 tcgatctcgg gcgacagcgt ggcgcgccag gtgccggccc gatcgaacgt gacaccctct
     3061 gcttcgtaga cgcctctggc tcccgatgcg atcgtcggcg cctcaccgga tgcgtccgtt
     3121 cccggcacgg gcacgtactt accggtggcg gtgggtcctg cttccggggc gccggatccg
     3181 gtcccgagat aggcgaacgc gaggtcgacc gtgccctgcg tgacgacgcg gacgccgtcc
     3241 gtatcgctcg ccgcgatgcc gacctggacc cgctgggggg cgccggtgta gtgccaggtg
     3301 ctggccatct gggcggcgaa ggtggtgtcc acgtcggacc cgcccgtcgg ctcggcggtg
     3361 caggacatgg ccccgaggag gagggccagg gtcgcgagcg agatgcgccg aacggtcatc
     3421 cgttccacct tctcacggcc gggaggaaga cccggagagg gctccctcga gcagcagcag
     3481 gaaccgtcgc cggtcatcgt gacgcccgta gctgccgagg gagggcccgg cccggacgac
     3541 gcggtggcac gggacgaaca gctcgatcgg ggagcgcgcg agcgcgcttc ccgccgcgcg
     3601 gccggcgcgg gtggcgcccg cggcggccgc cacgtcgccg taggtccaga gctcgccgta
     3661 cgggatcgtc cggacggtct ccagcactcg ccgggtgaag ccctctccca tctccgagag
     3721 atcggccggc gtctcgaacg ctcgcagccg gccgcggaag taggcgtcga tctcgcggcg
     3781 gatgggagcg gtccgccgcc ggtcctccac ggcgtcgagg tcgtcgtcgg gggcaccgtc
     3841 ctcgtcgaag tgcgtgcgca cgacgccggc atcactgagg aggatcgtga acggcccgag
     3901 cggggtctcg gcggtggtgc gccagagcgt ccagccctcg tccgccatcg gcgctagatc
     3961 gggggccacg ggaacgggcg atccggcccc ggctccggga accgaaccgc cgcggcgacc
     4021 gtatccgcgc gatccgctag tgccgcgagc tccgccggag cgaggaggtt ggcgagctcg
     4081 tcgcgcacct ctccaccgcg gatctcgccg gcgagacggc gcacgtcggc cctggtttcc
     4141 tcttcgaccg gatcgccgat gaagtcccag atcaccgtcc ggagtttcgg ctcgtcgctg
     4201 aagcacactc cgtgatccac caggaagatc cgtccgtcct ccccgagcag gcaatgtccc
     4261 gacttgcggt cggcgttgtt gacgaccatg tcgaacgccg cgactcggcg gaaatcctcc
     4321 gggaaccgct cgacgagggt gaagtagtgc tcctcgggat cgtgcgggat gaaccgctgc
     4381 accgacccca ccccgtacgg cccgtcacgg aggacggtgg cggggacgtt cggccagccc
     4441 agggcccgcg agacgacgta cgcggccacc tcacgggcac agagcgtccc ctccgggaag
     4501 tcccacagtg gcgcctcacc gcggcggggc ttgtagacgg cgaggagctc ctcctcggtg
     4561 ctcttgacgc gggcgaggaa cgtgtggttc gacgcgttgg gcagcagacc gagcacctcg
     4621 agctcgccgg tgcccaggac gcgagggacg gcggggtcgc tcagtcccgc ttcgagtgcc
     4681 cgttcatcgc gggacacgcg tggccctccg cgtcgatcgg gttgccgcag aactggcacg
     4741 tcggccggcc cctcgcggcg acctccgctc cgtgccgtga gagcgccagc atctgggtcg
     4801 gcgacgccca gatgcggatc gattccggct tcgctcccac ttccgctccg agcaccgagc
     4861 gcggatcgtc ctcttccagg tcctcgggat cgggccggaa ctcctcgacc tccaggagga
     4921 agaggtcccg atcctggtcg aagccgatcg acaggcggcc ggctcgccag cgcggatcga
     4981 tcggttcctc gagggccatc gccgaaccct cgagagccgg cgtcccggca gacccggccc
     5041 cgagctccgt cacgagctcg gcgctgggca cgtcctccaa cagctcgagc acggaggcgg
     5101 ccagcagctg cacctgctgc ttctccacga tcaccgtgac gagctcgacg cccgctcgtg
     5161 cctggatgta gaagacgcgg gctcccggct cgccgatggc gtcggcggtg atgcgatcga
     5221 ccggtcccag gtccatcgac ctatcctcgc agcttcctcc cacgaccgtc ccgagcggcg
     5281 gcgttcgccg ccttcggggg agccgctgtg aaccgatcca ggcttcccgt gtcgttcacc
     5341 agcaggatcc gcggaacgcc gtcgccgatg tgcacgacgg agacggatgc ggggtccacg
     5401 acgactcgtt ggaactggtc gagatgagcg cccgcgtagt gggacacgag catcccgacg
     5461 agatccccgt gggttccgac gacgacacgg ccgcgggggt gccgagccac gatccgctcg
     5521 atctggtcga ggaggcgtgc ctcggcgtcc acgaagctct cgccggaagg gaactgaaag
     5581 cgagaagggc tgcgctgcac cgtaccccag agcttggtgc gccgaaggga ggggagcgtg
     5641 cggcccgtcc agttgccggc gtccatctcg atcagcgctt cggaagtgcg catctcgagt
     5701 cggcgggccg tcgcgagtgg ctcaagggtt tcgatgcagc gttcgagggg gctcgagtag
     5761 atcgcggtga gccgaacccc ctcgaagc
//
LOCUS       DASUVE010000219         4198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_669703, whole genome shotgun sequence.
ACCESSION   DASUVE010000219 DASUVE010000000
VERSION     DASUVE010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669703"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VF036_04750"
     CDS             complement(<1..235)
                     /locus_tag="VF036_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018850153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264571.1"
                     /translation="MIEGSSKSPRAAPRSGTIARFVQLIAGLVLFGASLALLVRSELG
                     LDPWDVFHQGLSVATGLSIGVCTIVAGGVVLLLW"
     gene            complement(316..600)
                     /locus_tag="VF036_04755"
     CDS             complement(316..600)
                     /locus_tag="VF036_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264572.1"
                     /translation="MWYRLDLRVRADVDDESLHEVGILANTYAIVDEVMASRDPESGE
                     PRIIARIDAPSAEAALGALLTVIFQTSGHAGLAEEGSLNRVLIEREAPIR"
     gene            complement(663..1418)
                     /locus_tag="VF036_04760"
     CDS             complement(663..1418)
                     /locus_tag="VF036_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264573.1"
                     /translation="MEARRRWGPVPAALLVAAVALSACTPASPGRPEAPTGTPTAVPT
                     VATISELRAAIARWHRTSATITYRTERQRPGLPESVHQCLRAVVEERSGVAAALRYCD
                     PSGIVALVWDPPRRWRLDVEEAGTRTTAIVVGGHGLICLRVEPSCRSRPVHLILDDLP
                     FVALVTGVAETARIAGLSAEGPVTMTEDIAAGIPVRCFERRAGASAARWCFADDGALV
                     SLALETEGHAPTIIEATSVSDRVDDARFDPPGG"
     gene            1539..1916
                     /locus_tag="VF036_04765"
     CDS             1539..1916
                     /locus_tag="VF036_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264574.1"
                     /translation="MDERTTDTDDRTTATATEDMSEGSEDVLELPESGTEAGPLFADE
                     QSASYRSRWDEIQARFVDDPRGAVGEADELVSTVIGELESSFRSRRDSLEAGWQEGDE
                     TSTESLRLALQAYRSFFGRLLNT"
     gene            1996..3465
                     /locus_tag="VF036_04770"
     CDS             1996..3465
                     /locus_tag="VF036_04770"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX6264575.1"
                     /translation="MGRSVWQRSVTRDEQLVSLRHIRWSASRAARRVVLAVVVVSLAI
                     IGTVAPAGARSDRSVINRSLSQADASGDAVLTWSEHAGDAALASCLAPTGNPLLESRM
                     YAMTHVAIHDALNAIDRRYEPYAFFGHARRRTSTDAAVATAAHHVLVPVLGQFPSPPL
                     PQTCIDAGIASVEEDYEAALDAIPNGRQKRRGVRVGAASAAAILALRADDGSDTLLVD
                     TNYPQGDEPGEYRFTPGTPFAFAPGWGDVTTFVLRDSSQFRADPPYDVTSSAYTADYR
                     EVKRLGGDDITTPSQRTNEQTEIALFWVESSPLQWNRIARIVSADQGLDEWENARLFG
                     LLNMALADGYIGSFETTYLYNFWRPVTAIQLAATDGNPDTVADPNWTPLVTTPPIPDH
                     DSAHSVEGGAAAAVMRRFFGTDQISFETCSLTLPAGSTCNDASSVTRSFSSLSQAARE
                     NALARIYVGFHFRNAVNDGVEHGRKIGNRAANLFLLPVG"
     gene            3506..4198
                     /locus_tag="VF036_04775"
     CDS             3506..4198
                     /locus_tag="VF036_04775"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6264576.1"
                     /translation="MDRSDPSYKGQRGYNRFLLAIYDPWVLGFMTRAVWKVPVPPGVE
                     RYRRLMGRRHLDVGPGTGYFLEKAAPPAGTEITLLDPNPNVLRKTSRRLAAMHPVTVE
                     ADVMKPLPVEGPFDSAALNYVLHCLHGPQSNKAVAIRNIADVLTPDGVLFGGTVLGLE
                     ERHRPQARAFLRAANRLGDFDNLGDTAEGLRRILEESFETVEIEVVGSGANFIATGPR
                     SADSNRERHHGA"
BASE COUNT          714 a         1459 c         1431 g          594 t
ORIGIN      
        1 tccacagcag caggacgacg ccaccggcga cgatcgtgca cacgccgatc gagagaccgg
       61 tggcgaccga cagaccctga tggaagacgt cccacggatc gaggcccagc tcgctccgga
      121 ccaagagcgc gaggctggcg ccgaacagca ccaggccggc gatcagctgg acgaaccgtg
      181 cgatcgttcc gctccggggc gcggcacgcg gcgacttcga actcccctcg atcatcccgc
      241 tccatcatcg ccggtggaca tccacgcgcc ccacgagagg ccgctacacg ggagcacgga
      301 tccctgggat gcggactagc ggatcggtgc ttcgcgttcg atgagaaccc ggttcagaga
      361 gccctcttcc gccaacccgg catgcccgga ggtctggaag atcacggtga gcagggcgcc
      421 gagcgccgct tctgcgctcg gagcatcgat cctggcgatg atgcgaggct ccccgctctc
      481 ggggtcacgt gacgccatca cctcgtcgac gatcgcgtag gtgttcgcga ggatgcccac
      541 ctcgtgcagg gactcgtcgt cgacgtccgc gcggacacga agatcaagcc gataccacat
      601 cgccccgcgc accgtagcgc ggcacggcga tcccgtacag ccggtgagag gcagggtggg
      661 ggtcatccgc ctggcggatc gaagcgcgcg tcgtcgactc gatccgagac gctcgttgcc
      721 tcgatgatcg tcggggcatg gccctcggtc tcgagcgcga gcgagaccaa ggctccgtcg
      781 tcggcgaagc accagcgcgc ggccgacgca ccggctcgtc gttcgaagca tcggacgggg
      841 atgccggccg cgatgtcctc ggtcatcgtc accgggcctt cggccgagag gccggcgatc
      901 cgcgcggtct cggccacgcc ggtgaccagc gccacgaagg gaaggtcgtc gaggatcaga
      961 tggacggggc gtgatcggca ggacggttcc acacgaaggc agatgagccc gtggcccccg
     1021 acgacgatcg cggtggttct cgtgccggcc tcctcgacgt cgagccgcca gcggcgcgga
     1081 ggatcccata cgagcgcgac gatgccgctc ggatcgcagt agcgaagcgc cgccgcgacc
     1141 ccgctccgct cctcgaccac cgcgcgcaga cactgatgga ccgactcggg cagccctgga
     1201 cgctggcgct ccgtccgata cgtgatcgtc gcggacgtcc ggtgccacct cgcgatggcg
     1261 gcccgcagct ccgagatcgt cgcgaccgtc ggaacggcgg tcggtgtccc cgtcggcgct
     1321 tccggccggc ctggagacgc aggtgtgcac gcggacaacg cgaccgccgc gacaaggagc
     1381 gcggccggca cggggcccca tcggcgcctc gcttccatcg cggggcgagc gtagcccgca
     1441 cggatgcgcg tgggcagcgt ttgcccctca cctcgaacgg gcacatctgc agtagcgatc
     1501 ggatcgaggt cggacgacct cagcggaagg agcacaggat ggacgagcga acgaccgata
     1561 cggatgatcg gaccaccgcc acggcgacgg aggacatgtc cgaggggagc gaggacgtcc
     1621 tcgagctccc cgagagcggg acggaggcag ggcccctctt cgccgacgag cagagtgcct
     1681 cgtatcggag ccgctgggac gagatccagg cgcgattcgt ggacgatccc cggggtgccg
     1741 tcggggaggc cgacgagctc gtgagcaccg tgatcggcga gctcgagtcg tcgttccgct
     1801 cgcggaggga ctccctggaa gcgggctggc aagagggtga tgagacgtcg acggagagcc
     1861 ttcgcctcgc cctacaggcg tatcggtcct tcttcggccg cctgctcaac acgtagggaa
     1921 cctgatcgtc tcgatttgac tcggagcgaa acgaggttca ccctcggagc aacacttgta
     1981 cgagggggtg tccctatggg gcgcagcgtc tggcagcgaa gtgtgacccg tgacgagcag
     2041 ctcgtctccc tgcgccacat ccgatggagc gccagccgtg cggcgcggcg ggtcgttctc
     2101 gccgtcgtcg tggtgtcgct cgcgatcatc gggacggttg ctcccgcggg cgccaggtca
     2161 gatcggagcg tgatcaaccg atcactctcg caggctgacg catcgggaga cgcagtcctg
     2221 acatggagcg agcacgcggg cgacgcggcc ctcgcctcct gcctcgcccc caccggcaac
     2281 cccctgttgg aatcgcgcat gtacgcgatg acgcacgtgg ccatccacga cgccctgaac
     2341 gcgatcgatc ggcgctacga gccgtacgcc ttcttcggac acgcgaggcg ccgcacatcc
     2401 accgatgccg cggtcgccac ggcagcgcac cacgtcctcg tgccggtcct cggccagttc
     2461 ccgtccccac cgctcccgca gacgtgcatc gacgcgggga tcgccagcgt cgaggaggac
     2521 tacgaggccg cgctcgacgc gatcccgaac ggtcggcaga agcggagggg cgtgcgggtg
     2581 ggagccgcgt ccgccgccgc gatcctcgcg ctccgagccg acgacggttc ggacaccctc
     2641 ttggtggaca cgaactaccc acagggcgac gagcccggcg agtaccggtt cacgcccggc
     2701 accccgttcg cgttcgcgcc gggttggggg gacgtcacca cgttcgtgct gagagacagc
     2761 tcccagttcc gcgcggaccc gccctacgac gtgacctcga gcgcctacac cgcggactac
     2821 agagaggtga agcgcctcgg tggcgacgac atcaccacgc ccagccagcg cacgaacgag
     2881 cagacggaga tcgccctgtt ctgggtggag agctcgccgc tgcagtggaa ccggatcgcg
     2941 aggatcgtct ccgccgacca agggctcgac gagtgggaga acgcgcgact gttcggcctg
     3001 ctcaacatgg ccctcgcgga cggctacatc ggctcgttcg agacgacgta cctctacaac
     3061 ttctggcgac ccgtcacggc gatccagctc gcggccaccg acggcaaccc cgacaccgtc
     3121 gccgacccga actggacacc gttggtgacg acgcctccga tcccggacca cgactcggcg
     3181 cacagcgtcg agggaggcgc ggcggcagcg gtgatgcggc ggttcttcgg taccgaccag
     3241 atcagcttcg agacctgcag cctgacgctc ccggccggga gcacgtgcaa cgacgcgtcg
     3301 tccgtgacac gttcgttctc gagcttgtcc caagcggcac gggagaacgc gctcgctcga
     3361 atctacgtcg ggttccactt ccggaacgcg gtcaacgatg gggtcgaaca cggccggaag
     3421 atcggcaacc gtgcggcgaa cctgttcctg ctccccgtag gctaggcttc gcgcggctcg
     3481 cgcggggagg cggctcgagg aggagatgga tcgcagcgac ccttcgtaca agggccagcg
     3541 cggctacaac cgcttcctcc tcgcgatcta cgacccgtgg gtgctgggct tcatgacccg
     3601 cgcggtctgg aaggtcccgg tcccgcccgg cgtcgagcgc tatcgccggc tcatgggacg
     3661 acggcacctg gacgtcggcc ccggcacggg ctacttcctc gagaaggccg ccccgccggc
     3721 agggaccgag atcactctgc tcgatccgaa cccgaacgtg ctcaggaaga cgtcacgaag
     3781 gctcgcggcg atgcacccgg tcaccgtcga ggccgacgtg atgaagccac tgccggtcga
     3841 gggaccattc gactcggcgg cgctgaacta cgtgctccac tgcctccacg gaccccaatc
     3901 gaacaaggcc gtcgcgatcc ggaacatcgc cgacgtcctc acgcccgatg gcgtgctctt
     3961 cggcggcacc gtcctcggcc tcgaggaacg gcaccggccg caggctcgcg cattcctgcg
     4021 cgcggccaac cggctgggtg acttcgacaa cctcggggac acggccgagg ggctccgtcg
     4081 gatcctggag gagtcgttcg agacggtgga gatcgaggtg gtcgggtcgg gcgcgaactt
     4141 catcgccacg ggcccaaggt cagctgattc gaaccgggaa cggcatcacg gcgcgtga
//
LOCUS       DASUVE010000220         2746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_104176, whole genome shotgun sequence.
ACCESSION   DASUVE010000220 DASUVE010000000
VERSION     DASUVE010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104176"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2081)
                     /locus_tag="VF036_04780"
     CDS             complement(<1..2081)
                     /locus_tag="VF036_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX6264577.1"
                     /translation="MDVRSRLQALLAERILVLEGPKGTAIQTLELSEEDFRGDLLKDH
                     PRDIRGDNDVLGLTRPDIVESIHRDYLDAGAWILSTNTFNGSPVSQAEYGLEPLVYDM
                     NRAAAENARRALDGAEDRFVVGSIGPTNRTLSISPKVEDPAFRTITFDELTAAYAEQI
                     RGLVDGGVDVLQIETIFDTLNAKAAIAAAQDVAPDLPLWLSFTAVDQSGRNLSGQTAE
                     AFWLSIEHAEPMIVGVNCSLGAAQMRPYLEALSRIAPTYTACYPNAGLPNAMGLHDED
                     PEITSGYLGEFARAGLANVLGGCCGTTPEHIRQITEAVRDLPPRTVPEPDETFTSFSG
                     LEPFVVRPDTGFVMVGERQNITGSKKFRRLIESGDFQAAVDIAREQVQNGANLLDVNM
                     DTDLLDGPQAMTTYLNLLATEPEVARVPIMVDSSSWDVIEAGLKCVQGKGVVNSISLK
                     EGEEDFLDKARRVQRYGAAVVVMAFDEQGQADTVNRKVEISERAYRLLTQEAGYEPSD
                     IIFDPNILAIATGMEEHADYARSFIDATRIIKRRCPGVKISGGVSNLSFAFRGNDVVR
                     EAMHSAFLYHARQAGLDMAIVNAGQLPVYDDIPKDLLEHVEDLLFNRRPEATERMLTF
                     AQTVTGEAMRTEQDLTWREGSVEERLSHALVHGIDAFIEEDAEEARQGYERPLEVIEG
                     PLMDGMQIVGDL"
     gene            2229..2648
                     /locus_tag="VF036_04785"
     CDS             2229..2648
                     /locus_tag="VF036_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6264578.1"
                     /translation="MRRSVAALRAVPLFDGFSKRHLHRLAAEAEVVAFDADRPIVEEG
                     GAGEAMFVVLSGGARVTRGGRKVATLVPGDFFGELSALDGGPRTASIVSETPMELLRL
                     FRHTLRRMIEEEPSLAMGLLEGLARRLRQVQPGGPVT"
BASE COUNT          384 a          924 c          952 g          486 t
ORIGIN      
        1 aggtcgccca cgatctgcat cccgtccatc agtgggccct cgatcacctc gagcggccgc
       61 tcgtagccct gtcgcgcctc ttcggcgtcc tcctcgatga aggcgtcgat cccgtggacg
      121 agcgcgtggg agagccgctc ctcgaccgag ccttcccgcc acgtcaggtc ctgctcggtc
      181 cgcatcgcct cgcccgtgac cgtctgcgcg aacgtgagca tccgttcggt cgcttcgggc
      241 cggcggttga agagcaggtc ctccacgtgc tcgaggaggt ccttcgggat gtcgtcgtac
      301 acgggcagct gtcccgcgtt cacgatcgcc atgtcgagcc cggcctggcg tgcgtgatag
      361 aggaacgcgg agtgcatcgc ctcacggacc acgtcgttcc cgcggaacgc gaacgacagg
      421 ttcgagacgc cgccggagat cttcaccccg gggcatcgcc gcttgatgat gcgggtcgcg
      481 tcgatgaacg atctcgcgta gtccgcgtgc tcctccatcc cggtggcgat cgcgaggatg
      541 ttcgggtcga agatgatgtc cgacggttcg tagccggcct cctgggtgag cagtcggtac
      601 gcgcgctcgg agatctcgac cttccggttc acggtgtccg cctggccctg ctcgtcgaac
      661 gccatcacca cgacggccgc gccgtagcgc tgcacgcgtc gggccttgtc gaggaagtcc
      721 tcctcgccct ccttgaggct gatcgagttc acgacgccct tgccctggac gcacttcagg
      781 cccgcctcga tgacatccca gctggacgaa tccaccatga tcgggacccg ggcgacctcc
      841 ggctcggtgg cgagaaggtt caggtacgtg gtcatcgcct gcggaccatc caacaggtcg
      901 gtgtccatgt tgacgtcgag gaggttcgcg ccgttctgga cctgctcgcg tgcgatgtcg
      961 actgcggcct ggaagtcgcc gctctcgatc aggcgccgga acttcttcga gccggtgatg
     1021 ttctgccgtt ctcccaccat cacgaatccg gtgtcggggc gcacgacgaa gggctcgagg
     1081 ccgctgaacg acgtgaacgt ctcgtcgggc tcggggaccg tgcgcggtgg aaggtcgcga
     1141 accgcctcgg tgatctggcg gatgtgctcc ggggtcgttc cgcagcagcc tccgaggacg
     1201 ttggcgaggc ctgcacgcgc gaactctccg aggtagccgc tcgtgatctc cgggtcctcg
     1261 tcgtgcagtc ccatcgcgtt gggcagaccg gcgttggggt agcaggccgt gtaggtgggc
     1321 gcgatgcgtg agagcgcttc gaggtacggc cgcatctgcg cggcgccgag cgagcagttg
     1381 accccgacga tcatcggctc cgcgtgctcg atcgagagcc agaacgcctc ggcggtctgt
     1441 cccgagaggt tccgcccgct ctgatcgacg gccgtgaagg acagccagag cgggaggtcc
     1501 ggcgcgacgt cctgggcggc cgcgatcgcc gccttggcgt tcagggtgtc gaagatcgtc
     1561 tcgatctgga gcacgtcgac gccgccgtcg acgagtccgc ggatctgctc cgcgtaggcc
     1621 gctgtcagct catcgaacgt gatcgtgcgg aacgcgggat cctcgacctt gggagagatc
     1681 gagagcgtgc ggttcgtggg cccgatcgac ccgaccacga accgatcctc ggcaccgtcg
     1741 agcgcccgcc gggcgttctc ggccgcggcg cggttcatgt cgtagaccag gggctccagc
     1801 ccgtactcgg cctgcgagac ggggctcccg ttgaacgtgt tcgtgctgag gatccaggcc
     1861 ccagcgtcca gatagtcgcg gtggatcgac tcgacgatgt cgggcctggt gaggccgagg
     1921 acgtcgttgt cccccctgat gtcgcgcggg tggtccttca ggaggtcacc gcggaagtcc
     1981 tcctcggaca gctcgagcgt ctggatcgcc gtgcccttcg gaccctcgag cacgaggatc
     2041 cgctcggcca gcaacgcctg cagcctgctc cgaacgtcca cccgataacc ctaccgccga
     2101 gcacggatgc gactgcgagc ctcggtgcgt cggagtaccc tccgctcatg cgagtcgtgc
     2161 cgaccgggag atcggtgctg cggacgaggg aggcttcgac ctcctcccgc cggcgatcag
     2221 ccacccaggt gcgccgctcg gtggccgccc tccgcgcagt gccgttgttc gatgggttct
     2281 ccaagcggca tctccaccgt ctcgccgcgg aggccgaggt cgtcgcgttc gacgccgacc
     2341 ggccgatcgt ggaggaggga ggggcggggg aggcgatgtt cgtcgtcctg agcggggggg
     2401 cccgcgtcac ccgagggggt aggaaggtgg ccaccctggt ccccggcgac ttcttcggag
     2461 agctgtcggc gctggacggt ggcccgagga ccgcgtcgat cgtctccgag acgccgatgg
     2521 agctcctgcg gctgttccgg cacaccctgc gccggatgat cgaggaggag ccgtcgctcg
     2581 cgatggggct cctcgagggg ctcgcgaggc ggctccgtca ggtgcagccc ggcggaccgg
     2641 tcacctgatc aacccagcag ggtgtcgatc tccttcaggg tggcgtcgtc cagctcgatg
     2701 tctcccgccg acgcgttcgt ccgaacgtgc gccgggttgc gactcc
//
LOCUS       DASUVE010000221         3041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_104460, whole genome shotgun sequence.
ACCESSION   DASUVE010000221 DASUVE010000000
VERSION     DASUVE010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104460"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VF036_04790"
     CDS             <1..582
                     /locus_tag="VF036_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX6264579.1"
                     /translation="ERLATAIFPDLRVELLHGRMRPKDKDRVMDDFRTGRSDVLISTT
                     VIEVGVDVPNATVMLVENAERFGLAQLHQLRGRIGRGAHDSYCVLFDESDETNQDARA
                     RIEAMTRTTDGFELADEDLKLRGEGTLFDTKQSGMPDLKLARLAEDLDLVKRARTRAF
                     ELIEDDPELDDHPALLAELRERFESSIAWLFSA"
     gene            625..1221
                     /gene="rsmD"
                     /locus_tag="VF036_04795"
     CDS             625..1221
                     /gene="rsmD"
                     /locus_tag="VF036_04795"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX6264580.1"
                     /translation="MRVIAGTAKGTRLGRAPAGVRPVSDRVREGLFSSIGPWIEGSRV
                     LDLYAGTGAVGIEALSRGAEHATFVDSSPSAVAAVRDNLRRAGFEGRGAVRRSNVRTF
                     LERAGGPGFDLVFLDPPYEAGPAELDPVLAALDLSSLLRDGFTVVISRGSKSSNGVIP
                     LHWEAARRLSYGDSVVSLFRSRIPPEEAPGDMPGNLEV"
     gene            1221..1706
                     /gene="coaD"
                     /locus_tag="VF036_04800"
     CDS             1221..1706
                     /gene="coaD"
                     /locus_tag="VF036_04800"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX6264581.1"
                     /translation="MGLTALCPGTFDPVTNGHLDIIGRASQTFETVVVGALENPSKQP
                     MFTLEERVAMLKEACSGMSNVTVVSFRGLLVDLARSQEGDAAIVKGLRAVSDYEYEIQ
                     MAQMNHRLAGVETLFMPTSPKWSFLSSSLVKEVAQLGGNVAGLVPDHVRTALDDRLEE
                     R"
     gene            1706..2254
                     /locus_tag="VF036_04805"
     CDS             1706..2254
                     /locus_tag="VF036_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264582.1"
                     /translation="MDLAARLEQLDELVREAKSMPLSSSVLVNRDEILERIAEMKEGL
                     PEEIKRARWIVRDREELLTKARQDGEQIVEHAHEEQLRMARKEEIVARATTEADRVVT
                     EAEERARVMRTEAEDYVDAKLAQFENSIRKILEASQASTKSLNRTLDQVEVGRERLRG
                     PATVAEAEFRQEHDLFDEEEDA"
     gene            2251..2820
                     /locus_tag="VF036_04810"
     CDS             2251..2820
                     /locus_tag="VF036_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX6264583.1"
                     /translation="MIGAIDVRDLVGRPGVWRSEQVSGTIDDLETELAGLPADTPVTA
                     DLILESLAEGILASGRLRGVLSLRCARCLKGFEEELTVEVRELFAPFPHEDSDEYPLE
                     PEGFLDPDQMIRDLVGVELPFAPVCRPDCRGLCGRCGGDLNLDECTCTEPDIDPRWAG
                     LGELFEALHRSESDPGLAPDQSAERSPEQ"
     gene            2877..>3041
                     /gene="rpmF"
                     /locus_tag="VF036_04815"
     CDS             2877..>3041
                     /gene="rpmF"
                     /locus_tag="VF036_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004693428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX6264584.1"
                     /translation="MAVPKKKQSKTRGDKRAANWKAKPPAYSECPQCHQPKLPHRVCG
                     NCGYYAGRQAI"
BASE COUNT          519 a          996 c         1057 g          469 t
ORIGIN      
        1 gagcggctcg ccacggcgat cttccccgat ctccgggtcg agctgctgca cgggcggatg
       61 cggccgaagg acaaggaccg cgtgatggac gacttccgca ccggtcgctc cgacgtattg
      121 atctccacca ccgtgatcga ggtcggcgtc gacgtgccga acgcgaccgt gatgctcgtc
      181 gagaacgccg aacgattcgg tctcgcgcag ctccatcagc tccgagggcg gatcggccgc
      241 ggcgcgcacg actcgtactg cgtcctgttc gacgagtccg acgagacgaa ccaggatgcg
      301 cgcgcccgga tcgaagcgat gaccaggacg accgacgggt tcgagctcgc ggacgaagac
      361 ctcaagctcc gaggcgaggg cacattgttc gacacgaaac agtcgggcat gccggacctg
      421 aagctcgccc gtctcgccga ggatctcgac ctcgtgaaac gcgcccgaac ccgcgcgttc
      481 gagctgatcg aggacgaccc ggagctcgac gatcatcccg cgctcctcgc cgagttgcgc
      541 gagcggttcg aatcttcgat cgcctggctg ttcagcgcct gagggagaga ccgggtcccg
      601 ggccctcccc gtatcctcga tgtgatgcgc gtcatcgcgg gcaccgcgaa ggggacgcgc
      661 ctggggcggg cgccggcagg cgtccgaccc gtgtccgatc gtgtccgcga aggcctcttc
      721 tccagcatcg gtccgtggat cgaaggttcg cgcgtcctcg atctctatgc cggcacgggt
      781 gcggtgggca tcgaggcgct ctccagagga gcggagcacg cgacgttcgt cgacagctcg
      841 ccctcggccg tcgccgcggt ccgcgacaac cttcggcgag ccggcttcga gggtcgtggc
      901 gcggtccgtc ggtcgaacgt tcggacgttc ctcgagcggg ccggcgggcc gggtttcgac
      961 ctcgtcttcc tcgatccgcc gtacgaagcg ggccccgccg aattggatcc ggttctggcc
     1021 gccctggacc tgagttccct gctcagggac ggtttcaccg tggtcatcag ccgcggatcg
     1081 aagagttcca acggtgtgat tccgctacac tgggaagccg cgaggcggct ctcctacgga
     1141 gatagcgtcg tctcgttgtt ccggtcacgg atcccgccgg aagaagcccc aggggacatg
     1201 ccgggaaact tggaggtgtg atgggcctga ccgccctgtg tccggggacg ttcgaccccg
     1261 tgacgaacgg tcatctcgac atcatcggac gggcatcgca gaccttcgag acggtggtcg
     1321 tcggtgcgct ggagaacccc tccaagcagc cgatgttcac gctcgaggag cgcgtcgcga
     1381 tgctcaagga ggcgtgctcc ggcatgtcca acgtcaccgt ggtctcgttc cggggtctcc
     1441 tcgtggatct cgcgcgttcc caggagggcg atgccgcgat cgtcaaggga ctccgggccg
     1501 tctcggacta cgagtacgag atccagatgg cgcagatgaa tcaccgactc gccggggtgg
     1561 agacgctgtt catgcccacg agcccgaagt ggtccttcct ctcctcctcg ctcgtgaagg
     1621 aggtcgccca gctcggaggg aacgtcgcgg gactcgtccc ggaccacgtg cggacggcgc
     1681 tcgatgaccg gttggaggaa cgctgatgga cctcgcggcc cgactcgagc agctcgacga
     1741 gctcgtgcga gaggcgaagt cgatgccgct gtcgtcgtcc gtgctcgtca accgcgacga
     1801 gatcctcgag cggatcgccg agatgaagga agggctgccg gaagagatca aacgcgctcg
     1861 gtggatcgtg cgggaccgag aggagctgct cacgaaggcg cgccaggacg gcgagcagat
     1921 cgtcgaacac gcgcacgagg agcagctccg gatggcacgc aaggaggaga tcgtcgcccg
     1981 ggcgacgacc gaggcggatc gcgtggtcac ggaggctgag gagcgtgccc gcgtgatgcg
     2041 gaccgaggcc gaggactacg tcgacgccaa gctcgcgcag ttcgagaact cgatccgcaa
     2101 gatcctcgag gcttcgcagg catcgacgaa gtcgctgaac cggacgctcg atcaggtcga
     2161 ggtcgggcga gagcggctcc gcgggccggc gaccgtcgcg gaggcggagt tccggcagga
     2221 gcacgacctc ttcgacgagg aggaggacgc gtgatcggcg cgatcgacgt gcgcgacctc
     2281 gtgggacggc ccggcgtgtg gcggtccgag caggtctccg gcacgatcga cgatctcgag
     2341 acggagctcg ccggtctgcc cgcggatacc ccggtcacgg ccgacctcat cctcgaatcc
     2401 ctcgcggaag gcatcctcgc cagcggtcgg ctccgagggg tgctgtcgtt gcggtgcgcc
     2461 cgatgcctga aggggttcga agaggagctc accgtcgagg ttcgcgagct gttcgccccg
     2521 ttcccccacg aggactccga cgagtacccg ctggagccgg aaggcttcct cgaccccgac
     2581 cagatgatcc gcgacctcgt cggtgtcgag ctgccgttcg ccccggtgtg ccgtcccgac
     2641 tgccggggtc tgtgcggccg ctgcggcggc gacctgaacc tcgatgagtg cacctgcacg
     2701 gagcccgaca tcgatccccg ctgggcaggt ctcggcgagc tgttcgaagc cctgcatcgc
     2761 tcggagtcgg acccgggcct ggcccccgac cagagcgcgg agcggtctcc ggagcagtga
     2821 tcgacacgaa gccagagact ccacgacgag acccgaaccc ctgaagggat gaagagatgg
     2881 cagttcccaa gaagaagcag agcaagacgc ggggtgacaa acgcgccgcg aactggaagg
     2941 cgaaaccgcc cgcgtactcg gaatgcccgc agtgtcatca gcccaagctt ccccaccgcg
     3001 tgtgcgggaa ctgcgggtac tacgcgggcc ggcaggccat c
//
LOCUS       DASUVE010000222         2986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_670891, whole genome shotgun sequence.
ACCESSION   DASUVE010000222 DASUVE010000000
VERSION     DASUVE010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670891"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VF036_04820"
     CDS             <1..663
                     /locus_tag="VF036_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6264585.1"
                     /translation="SPTGLPVGLNWNLPYEESIALLRPGATLVLFTDGLVDRRDVDVQ
                     VGLERLRAVAADPGDGDLDEMCGALLERLVPPDASDDVAILVARLEPVRDRLSLTIPA
                     DPERLGAVRRNLGRWLSGHQVPKEDADDVILASSEACANSIEHAYGPGEGSVDIDAEV
                     HDGEVTIVVRDKGRWRAPSNGDRGRGLMFIEACTDSYTLTRGDAGTEVRMRRRVGRSR
                     TA"
     gene            660..1016
                     /locus_tag="VF036_04825"
     CDS             660..1016
                     /locus_tag="VF036_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6264586.1"
                     /translation="MNDLAAFTVERDDAVVVGALSGEVDMSNAALLERAITEAVPNSA
                     RGLVLDLSALSYIDSSGVRLVLSLAGSLRWRGQELVIAAPEGSSCRRVLTLAGIESSV
                     ALEATEEAALARFPAS"
     gene            complement(1063..1458)
                     /locus_tag="VF036_04830"
     CDS             complement(1063..1458)
                     /locus_tag="VF036_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6264587.1"
                     /translation="MSTSSRVVSSHATAFVEARRFVRDSVGPVPSRIVDDAILLTSEL
                     VTNAVRHAGLDDQDEIEVTVSVDPRILRITVRDRGPGFDPEAMVPRSDEGGWGLDLLR
                     RLSSRWSVDRDSAGTDVWFEIDLPDGSGS"
     gene            1497..2378
                     /locus_tag="VF036_04835"
     CDS             1497..2378
                     /locus_tag="VF036_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6264588.1"
                     /translation="MTAPTALYPSGEAPEHRGLSDRDGGTDGIRDEARDGVRLYLEDD
                     GGGGTPVLILGGFLDPIELVRRAPIARALAASTDEFRLIFVDHRGHGRSDKPHDADAY
                     AMPLRVADVVAVLDELQIRRAHLLGISWGGRLCFGIGQHAPERVRSLIAIGQHPYEID
                     RDGPLARVVGDALEASKQRGVEALVEAFEAIAGRYPDHVRSTYLACDAEAMRAAWEEA
                     VAEGPVGEDLGSWTVRCLICAAEDDADFFEDARRAASEIPDAEFVAIPGTDHLGVDTA
                     RVDPVLPAVLRTLRDPN"
     gene            complement(2393..>2986)
                     /locus_tag="VF036_04840"
     CDS             complement(2393..>2986)
                     /locus_tag="VF036_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase"
                     /protein_id="HEX6264589.1"
                     /translation="LPSSWIADFRRLYDFNEAGRADLAVPEAKFNRAMRIDTTLTNPL
                     ANLPGFPNGEDNLAFRNLTRANMVKLATGQQMAAFLKNKGVTLTKLTTPQIRNGLNGA
                     TLEGLNQAQRTALLADTPLWFYILREAELNQGRLKGVGARIVAETFHRAMEGSTISIV
                     REPGWRPTLGPDNTTFRMVDLLLFAFEGKRNLLAPVT"
BASE COUNT          450 a          989 c         1057 g          490 t
ORIGIN      
        1 agcccgaccg gtctgcccgt cggactcaat tggaacctgc cgtacgagga gtcgatcgcg
       61 ctcctgcggc cgggggcgac gctcgtgctc ttcacggacg ggctcgtcga ccggagggac
      121 gtcgacgtgc aggtgggact cgagcgtctc cgcgcggtgg cggcggatcc gggcgatggc
      181 gacctcgacg agatgtgcgg cgccctcctc gagcgcctcg tgccgcccga cgcctccgac
      241 gacgtggcga tcctcgtcgc ccggctggag cccgttcggg accggctctc gctcacgatc
      301 ccggccgacc cggagaggct cggcgccgtc cggcgcaacc tcggccgatg gctctccggg
      361 caccaggtgc cgaaggagga tgccgacgac gtgatcctcg caagctcgga ggcgtgcgcg
      421 aactcgatcg agcatgcgta cgggccgggg gagggatccg tggacatcga tgccgaggtg
      481 cacgacggcg aggtcacgat cgtggtccgc gacaaaggcc ggtggagggc gccgagcaac
      541 ggcgatcgcg ggcgcggact gatgttcatc gaggcgtgca cggattccta cacactcaca
      601 cgaggcgatg ccggaacgga ggttcggatg cggcggcggg tcggtcggtc ccggaccgca
      661 tgaacgacct ggcggcgttc acggtcgagc gcgacgacgc cgtcgtcgtc ggggcgcttt
      721 ccggggaggt cgacatgtcg aacgccgccc tgctggagcg ggcgatcacg gaggccgtgc
      781 cgaacagcgc gaggggcctc gtgctcgacc tgtcggccct ctcctacatc gacagttccg
      841 gcgtccggct cgtgctctcg ctcgccggga gcctcaggtg gcgaggacag gagctcgtga
      901 tcgcggcgcc cgagggatca tcgtgcagac gtgtgctcac cctcgcgggg atcgaaagtt
      961 ccgtcgcgct cgaagcgacc gaggaggccg cgctcgctcg attcccggcg agctgacggt
     1021 gcatatcgga ggtcgccgtc gcggtgggat cgaggccggg gctcaggacc ccgacccgtc
     1081 ggggagatcg atctcgaacc agacgtccgt gcccgcgctg tcccgatcca cactccaacg
     1141 agaggacagt cgccggagga gatcgagccc ccagccaccc tcatccgacc gggggaccat
     1201 cgcctccgga tcgaaacccg gaccccgatc ccgcaccgtg atccgcagga tccgtgggtc
     1261 gaccgacacg gtgacctcga tctcgtcctg atcatcgagg cccgcatgcc ggacggcgtt
     1321 cgtgaccagc tcgctggtga ggaggatcgc gtcgtcgacg atccggctcg ggaccggccc
     1381 cacgctgtcc cgaacgaatc ggcgggcctc cacgaaggct gtggcgtgcg acgatacgac
     1441 ccgggaactg gtggacactg gcctccttcc cgtgaggtcg gcggcatcgg tgcggggtga
     1501 ccgctcccac ggccctttac ccttccggcg aggcccccga acatcgtgga ctgagtgacc
     1561 gagatggtgg cacggacggc atccgcgacg aagcgcgtga cggagtccgc ctgtatctcg
     1621 aggatgacgg cggtgggggc acccctgttc tgatcctcgg tggattcctc gatccgatcg
     1681 agctcgttcg ccgggcccct atcgcgcggg cgctcgcggc atccaccgat gagttccggc
     1741 tcatcttcgt cgatcatcgg ggtcatgggc ggagcgacaa gccacacgac gccgatgcgt
     1801 atgcgatgcc acttcgcgtc gcggacgtcg tcgccgtgct cgacgagctg cagatccggc
     1861 gggcgcatct cctcggcatc tcgtggggcg gtcggctctg cttcgggatc ggccagcacg
     1921 cacccgagcg tgtccgttca ttgatcgcca tcggtcagca tccgtacgag atcgatcggg
     1981 acggccccct cgcgcgtgtg gtcggggacg cgttggaggc gtcgaagcag cgaggggtcg
     2041 aagccctcgt cgaggccttc gaggcgatcg cggggcgcta cccggatcac gtgcgatcga
     2101 cgtacctcgc ctgtgacgcg gaggcgatgc gagcggcatg ggaggaggcc gttgcggaag
     2161 ggcccgtggg cgaagacctg ggctcgtgga cggtgcgatg tctcatctgc gcggcggagg
     2221 acgacgcaga cttcttcgag gacgctcgac gcgcggcctc cgagatcccc gacgcggagt
     2281 tcgtcgcgat cccgggaacc gatcatctcg gcgtggacac cgctcgcgtc gatccggtcc
     2341 tccccgccgt gttgcgaacg ctccgcgatc cgaactgagg ccacgccgtg acctacgtca
     2401 cgggagcgag aaggttcctc ttcccctcga acgcgaacag cagcaggtcg accatgcgga
     2461 acgtcgtgtt gtcgggaccg agcgtcgggc gccagccggg ctcgcgaacg atcgagatcg
     2521 tgcttccctc catggctcgg tggaacgtct ctgccacgat gcgggcgccg actcctttca
     2581 accgcccctg gttcagctcc gcctcccgca ggatgtagaa ccacagcgga gtatccgcca
     2641 ggagcgccgt ccgctgtgcc tggttgagcc cttcgagcgt ggcgccgttc aggccgttcc
     2701 ggatctgcgg ggtcgtgagc ttggtcaggg tgacgccctt gttcttcagg aacgcggcca
     2761 tctgctgacc ggtggcgagc ttcaccatgt tcgctctggt gaggttgcgg aacgcgaggt
     2821 tgtcctcgcc gttggggaat ccggggaggt tcgccaacgg gttcgtcagc gtggtgtcga
     2881 tccgcatcgc tcggttgaac ttcgcctcgg gaacagcgag gtccgccctc ccggcttcgt
     2941 tgaagtcgta gagacgccgg aagtccgcga tccagctgct cggaag
//
LOCUS       DASUVE010000223         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_484054, whole genome shotgun sequence.
ACCESSION   DASUVE010000223 DASUVE010000000
VERSION     DASUVE010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484054"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /gene="alaS"
                     /locus_tag="VF036_04845"
     CDS             complement(<1..1576)
                     /gene="alaS"
                     /locus_tag="VF036_04845"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX6264590.1"
                     /translation="MEGDRIRELFLSFFEERGHRRVPSSSLIPPPESGLLLANAGMNQ
                     FIPIFLGQAQPEYPRAVTVQKVMRTNDIDNVGTDARHLTFFEMLGNFSFGDYFKADAI
                     AWAHQLVTEGYGIDHDRLWVTVYEEDDEAVQGWVDGVGLSPDRIVRRGMTDAEGELAN
                     YWHTHAAGPGGPCSEIFVDRGAEYGPEGGPDVDEERFMEIWNLVFIQDRIDQELRFVE
                     PLPAKNVDTGSSLERVAVVLQNVDSVFETDLLRPLLEVAESLSGRTHGRDGHVDTSLK
                     IIAEHGRATAFLIADGVQPANEGRGYILRRMLRRMVSHARALGIERAVMDPIIDVVVA
                     TFGDAYASLRDNEAFVRQVAGSEEERFAGTLRQGLVLFDDAKGRARDGRITGDDAFKL
                     SDTFGFPLQLTEELAAEAGLSVDVDRFAALLEEQRDRARRAAKKVPIGLDARATPPTT
                     FVGYETLESDATIVTMLDPEFRELPVAEEGQDVRIFLDRTPFYAEGGGQVGDRGLIRT
                     PSGVIRVDDTVPAGDRS"
     gene            complement(1621..1890)
                     /locus_tag="VF036_04850"
     CDS             complement(1621..1890)
                     /locus_tag="VF036_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264591.1"
                     /translation="MRRSFWVALGLGAGATGAVVASRWARRQAHKVKPTTLAREARGG
                     LMDLSKLVAESIEEGKRAMEERERELRAELDASKREPGPDEPTAA"
BASE COUNT          296 a          750 c          652 g          319 t
ORIGIN      
        1 tcgatcgatc gcccgcgggc acggtgtcgt cgaccctgat cacgcccgag ggcgtccgga
       61 tcaggccgcg atcgcccacc tggccacccc cttccgcgta gaacggggtg cggtcgagga
      121 agatccggac gtcttgaccc tcctcggcca cgggcagctc gcggaactcc ggatcgagca
      181 tcgtcacgat cgtggcgtcg gactccaggg tctcgtagcc cacgaacgtc gtgggaggag
      241 tcgccctggc gtcgaggccg atcggcacct tcttggcggc gcgccgcgcc cgatcgcgct
      301 gctcctcgag caacgccgcg aaccgatcga cgtccacgga caggcccgcc tccgccgcga
      361 gctcctccgt gagctgcaag gggaacccga acgtgtccga gagcttgaag gcgtcgtctc
      421 ccgtgatccg tccgtcacgg gctctcccct tcgcgtcgtc gaacaggacg agcccctgcc
      481 ggagcgtgcc cgcgaaccgt tcctcctcgg accccgccac ctgccgaacg aacgcctcgt
      541 tgtcccggag cgacgcgtac gcgtcgccga acgtcgccac cacgacgtcg atgatcggat
      601 ccatcacggc gcgctcgatc ccgagcgccc gggcgtgcga gaccatccgg cgcagcatcc
      661 gccgcaggat gtagccgcgt ccctcgttcg ccggctggac cccgtccgcg atcaggaacg
      721 cggtcgcccg gccatgttcg gcgatgatct tcagggacgt gtcgacgtgg ccgtcccggc
      781 cgtgcgtgcg acccgacagg gactccgcca cctcgagcaa cggacggagc aggtccgtct
      841 cgaagacgct gtccacgttc tgaagcacca ccgccacacg ctccagcgac gagcccgtgt
      901 cgacgttctt cgcggggaga ggctcgacga aacggagctc ctggtcgatc cgatcctgga
      961 tgaagacgag gttccagatc tccatgaacc gttcctcgtc cacgtccggg ccgccctccg
     1021 gtccgtactc ggccccgcga tcgacgaaga tctcgctgca cggaccgccc gggccggccg
     1081 cgtgggtgtg ccaatagttc gcgagctcgc cctccgcatc cgtcatcccc cggcggacga
     1141 tgcggtcggg ggagaggccc acgccgtcga cccagccctg caccgcctcg tcgtcctcct
     1201 cgtacaccgt cacccagagc cggtcgtggt cgatcccgta tccctcggtg acgagctggt
     1261 gcgcccaggc gatcgcgtcg gccttgaagt agtcgccgaa cgagaagttg ccgagcatct
     1321 cgaagaacgt gaggtgccgg gcatccgtac cgacgttgtc gatgtcgttc gtgcgcatca
     1381 ccttctggac ggtgacggcg cgcgggtact cgggctgggc ttgtcccagg aagatcggga
     1441 tgaactggtt catccccgcg ttcgcgagga gcagtccgga ctcgggaggt gggatcaggg
     1501 acgaactcgg cacgcgcctg tgccctcgct cctcgaagaa cgacaggaac agctcgcgga
     1561 tccggtcccc ctccacgggc ggagtctacc ggtgcgtctc cgcagccctc ggaccgggcc
     1621 tcaggccgcg gtcggctcgt ccggaccggg ttcgcgcttc gacgcgtcga gctccgcgcg
     1681 gagctcgcgc tcccgctcct ccatcgcccg cttgccctcc tcgatcgact ccgcgacgag
     1741 cttggaaaga tccatcagac cgcctcgggc ctcgcgcgcc agcgtcgtcg gcttgacctt
     1801 gtgcgcctga cgtcttgccc atcgcgacgc gacgacggcg ccggtcgcgc cggcgccgag
     1861 gcccagcgcg acccagaagc tgcggcgcat gtcagggcgc cggcgcgccc ggcgccgggg
     1921 atcccttcga ccgcttcttg ctgaacgaat cggccgcctt cttcacgccg gcgcccacgc
     1981 tcaggaactt cacgatcggg ctggaggcgg cgtgctc
//
LOCUS       DASUVE010000224         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_484401, whole genome shotgun sequence.
ACCESSION   DASUVE010000224 DASUVE010000000
VERSION     DASUVE010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484401"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VF036_04855"
     CDS             <1..786
                     /locus_tag="VF036_04855"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003625901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6264592.1"
                     /translation="MVVIYLGSLAILLVSSFWRLDVFTGEVVRDPSLVNFQTLLDNDV
                     YRTITLRTVGIALAVTLTDIVLAFPLAYYAARLATPRMRNALLIAVVLPLWANYLVRV
                     FAWRLILTPNGFLNWLVESVGLGSLQVGKSNWGIWITSVYLWLPFTLLPIYAAIERVP
                     DSLLEASGDLGGRGWFTFRRVVFPLIVPGIVAGSIFSFSLTLGDYIVPELVGNTQFIG
                     NVIYDNVGVAGNVPLAAAYALVPIAVMAVYLFLAKRVGAFEAL"
     gene            789..1604
                     /locus_tag="VF036_04860"
     CDS             789..1604
                     /locus_tag="VF036_04860"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009713225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6264593.1"
                     /translation="MESRASRILIGVGTLVTLVFLYVPLAIVVIYAFNDEIGQAWPIQ
                     DYTWKWFAIAFRTAAVREALLNSVQVALFATVVALILGSAAAFAVHRFRFFGRDAVSF
                     LLVLPIALPGIVTAIALNSAINTFGIPFGVLTIVIAHATFCVVVVYNNVLARLRRTPT
                     SLIEASMDLGADGWQTFRYVTFPAIRTAVLAGALLAFALSFDEIVVTNFTAGSTLTIP
                     KFIYNSIKLPRNRPVVNVVALVLVLLMLIPVYIAQRLAGSSSETAVGRVTAEP"
     gene            complement(1611..2081)
                     /locus_tag="VF036_04865"
     CDS             complement(1611..2081)
                     /locus_tag="VF036_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HEX6264594.1"
                     /translation="MNPDPAPQVPEAPARQAEASEESITEPAKLLRIASMVRELLEET
                     RQASLDEPGRIRLRQIHERAIGELSEVLSPDLKDELSTLAPPMQGVPTDAEIRVAQAQ
                     LVGWLEGLFHGIQAALFAQQAAARAQFEELRRRGLPAGQAPQEPGTDGNRGQYL"
BASE COUNT          289 a          754 c          734 g          429 t
ORIGIN      
        1 atggtcgtga tctacctcgg atcgctcgcg atcctgctcg tctcgtcgtt ctggcggctc
       61 gatgtcttca ccggcgaggt cgtgcgcgac ccgagcctcg tgaacttcca gacgctcctg
      121 gacaacgacg tctatcggac gatcaccctc cggaccgtgg ggatcgcgct ggccgtcacg
      181 ctgacggaca tcgtgctggc cttcccgctc gcgtactacg cggcacggct cgcgaccccg
      241 cggatgcgga acgccctgct gatcgcggtc gtcctgcccc tgtgggccaa ctatctggtc
      301 cgcgtcttcg cgtggaggct gatcctcacg ccgaacggct tcctgaactg gctcgtggag
      361 tcggtcggtc tcgggtccct ccaggtcggc aagtccaact gggggatctg gatcacgtcc
      421 gtctacctct ggctcccgtt cacgcttctg ccgatctacg cggcgatcga gcgcgtgccg
      481 gattccctcc tggaagcgtc gggcgacctc ggcggccggg ggtggttcac gttccgccgt
      541 gtcgtgttcc ccttgatcgt ccccgggatc gtggcgggtt cgatcttctc gttctccctc
      601 accctcggcg actacatcgt gcccgagttg gtcgggaaca cacagttcat cgggaacgtg
      661 atctatgaca acgtaggtgt tgccggcaac gttccactcg ccgccgcgta cgccctcgtg
      721 ccgatcgccg tcatggccgt ctatctgttc ctcgcgaagc gcgtgggcgc gttcgaggcc
      781 ctctgaggat ggagtcgcgc gcctctcgga tcctaatcgg tgtcggcacg ttggtcacgc
      841 tggtgttcct gtacgtgccg ctcgcgatcg tggtgatcta cgcgttcaac gacgagatcg
      901 ggcaggcatg gccgatccag gactacacgt ggaaatggtt cgcgatcgcg ttccggacgg
      961 ccgccgtcag ggaagcgctg ctcaactcgg tccaggtggc gttgttcgcg acggtggtcg
     1021 cgctgatcct tggaagcgcg gccgccttcg ccgtgcaccg gttccggttc ttcgggcggg
     1081 acgcggtctc gttcctcctc gtactcccga tcgcgctgcc ggggatcgtg acggcgatcg
     1141 cgctgaactc ggcgatcaac acgttcggga tcccgttcgg cgtgctcacg atcgtgatcg
     1201 cgcacgcaac gttctgcgtc gtcgtcgtgt acaacaacgt actcgcgagg ctccggcgga
     1261 cgccgacgtc gctgatcgag gcgtcgatgg atctcggcgc cgacggctgg cagacgttcc
     1321 gctacgtgac gttcccggcg atccggaccg cggtgctcgc gggagcgctg ctcgcgttcg
     1381 cgctgtcgtt cgacgagatc gtcgtcacga acttcacagc gggaagcacc ctgacgatcc
     1441 cgaagttcat ctacaacagc atcaaactac cgaggaaccg gccggtggtg aacgtcgttg
     1501 ctctggtcct cgttctcctg atgctgatcc ccgtctacat cgcgcagcga ctcgcgggct
     1561 cttcgtcgga gacagcggtg ggccgggtga cggccgagcc gtaggcccgg ctacaggtac
     1621 tgcccgcggt tgccgtccgt gccgggctcc tgcggcgcct ggccggcggg gagcccgcgc
     1681 cggcgcagct cctcgaactg cgcccgggcc gcggcctgct gcgcgaagag tgcggcctgg
     1741 atgccgtgga acaggccctc gagccatccg acgagttgcg cctgcgcgac ccggatctcg
     1801 gcgtcggtcg gcacgccctg catcggcggc gcgagcgtcg agagctcgtc cttcaggtcc
     1861 ggcgacagga cctcggacag ctcgccgatc gcgcgctcgt ggatctggcg aaggcggatc
     1921 cgtccgggtt cgtcgagcga cgcctgccgc gtctcctcga gcagctcccg gaccatgctc
     1981 gcgatccgga ggagcttcgc gggctcggtg atcgactcct cgctcgcctc ggcctgccgg
     2041 gcgggggcct cggggacctg gggcgcgggg tccgggttca ccccccgagc ctacaaggcc
     2101 ccgggtcagt cgcggacgag ctcaccgaac agcagcgcct gcgccagcgt ggcgcgttcg
     2161 agctcgtcga gatccacgct ctcgtcgatc gagtggtact ggctct
//
LOCUS       DASUVE010000225         5929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_295233, whole genome shotgun sequence.
ACCESSION   DASUVE010000225 DASUVE010000000
VERSION     DASUVE010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295233"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /gene="meaB"
                     /locus_tag="VF036_04870"
     CDS             <1..877
                     /gene="meaB"
                     /locus_tag="VF036_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX6264595.1"
                     /translation="EDDAPELAELSAGIYPETGRAATIGLTGAPGVGKSTLAGELVRI
                     TRAADLRAAVLAVDPTSPYTGGALLGDRVRMQDHAVDPGVFIRSMATRGHLGGMALAA
                     PEAVRILDAAGYDVIFVETVGVGQAEVDVAAATDTAVVVMAPGMGDAVQMAKAGILEA
                     ADVFVVNKADRDGAPDVVKELRQMLHMGAERDWDPPVLMATAAKAEGVDEAWAAIREH
                     RDGAEASGALAAKRRARLLREAESLAAERFRVKAAEALEQDPALGDDLAERRIDPYAA
                     AQELLRRAKGLDQLA"
     gene            complement(941..1402)
                     /locus_tag="VF036_04875"
                     /pseudo
     CDS             complement(941..1402)
                     /locus_tag="VF036_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
     gene            1880..3463
                     /locus_tag="VF036_04880"
     CDS             1880..3463
                     /locus_tag="VF036_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013288195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX6264596.1"
                     /translation="MSGEPVPAIVGPDDLEGWEPDEKLGEPGAFPFTRGAYPSMYRGR
                     LWTMRQFAGYGTPAETNARYRFLTDHGQTGLSVAFDMPTLMGLDSDDPRSEGEVGRCG
                     VATDSLDDFETLFDQIPLGDVTTSMTISGPAVVVFAFFLATAEEQGVDWSTLDGTLQT
                     DIFKEYIAQKEWVFPPRPHLRLIGDLMAFCAERVPKWHPISVSGYHIREAGATAAEEL
                     AFTLADGFAYVELGMQRRLDVEAFAPALSFFFNAHIDFFEEIAKLRAARRIWARWMRD
                     RYGATSPEAMRLRFHTQTAGVSLTAQQPMNNVVRTAIEALAAVLGGTQSLHTNALDEV
                     LALPTEDTAKLALRTQQVIAFESGVPDVIDPLGGSWFVEDATDRLEARAEEIFTEIER
                     MGEGAMLEGVLEGIEQGWFQRRIAESAFQEQKRYESGDLVKVGVTDYVDANENPIDTL
                     VIGEEAERTQLASVRRTRAARDAAAADDALIALKEATTTDANLMEPLIDCARARCTEG
                     EIVGALAAVFGTYRETPAF"
     gene            3511..4506
                     /locus_tag="VF036_04885"
     CDS             3511..4506
                     /locus_tag="VF036_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX6264597.1"
                     /translation="MDMRRGALAAALLLLLTGACGSGGPGSGAGVQVVTTVSPITNIV
                     QNIGGDRVSVTGIVPEGVNSHTFEPAPSDAAVMEDADIVFINGLHLEEPTRELAEANV
                     GDGVPIVQLADRTISEDEYIFDFSFPRSEGFPNPHLWTNPPYAKDFAEIVRDELSELD
                     PEGSSTFGENFEAFADRVDALDAAVRSATETVAEEDRKLLTYHDSFPYFAREYGWEVV
                     GAIQPSDFAEPTPQEIVGLIEQIRAESLPAIFGSEVFPSPVLEQIAAETGATYVDDLR
                     DDDLPGEAGDPDHSYLGLMVFDFTTIVRALGGDPAAFEGLDVTNLAPDAAEGTRY"
     gene            4506..5315
                     /locus_tag="VF036_04890"
     CDS             4506..5315
                     /locus_tag="VF036_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX6264598.1"
                     /translation="MDELVRFDRVTCRYGRDPVLVNVDLEIGAGEFIGVVGPSGSGKS
                     TLLKAIAGSVEPIAGQVRRDPELSIGYVPQVETVNWYFPVTVREAVLMAKIDGRRVPW
                     PSKQESSQAQEVLERLGLGGLSDRHIRELSGGQQQRVFIARAMLGRPRLLLLDEPTSG
                     VDVRTRHDVMHVIHDLHHEGLAIVLTTHDLNGIASHLPRLVCLNREVIATGTPRQVIT
                     SEVLERTYGAPLEVLSHGGMPVVLEQYPELVEEEEGARVLPLREDHHEHPA"
     gene            5299..>5929
                     /locus_tag="VF036_04895"
     CDS             5299..>5929
                     /locus_tag="VF036_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX6264599.1"
                     /translation="MSTLLEPFGYEFFQRALLVATITGALCGLIGVYVVLRGMSYIGH
                     GLSHAIFGGAVASYIVGVNVLLGAGLWGLVAALFINKVARRRSIGADAAIGAITSASF
                     AIGIILISTQRTFVVNFEALLWGNVLGVTGVDVLVVLAVAAGSILFVFLGYRQLLFST
                     FDPEVAEVSGVRTARVDIGLAVMLTATIAATMNVIGVTMIAAMLVIPPVI"
BASE COUNT          930 a         1998 c         2095 g          906 t
ORIGIN      
        1 cgaggacgac gcgccggagc tggccgagct gtcggcgggg atctacccgg agaccggtcg
       61 tgcggccacg atcggcctga ccggcgcgcc aggcgtgggc aagtccacgc tcgcggggga
      121 gctcgtccgg atcacccgtg cagccgatct ccgcgcggcc gtcctcgccg tggatcccac
      181 ctcgccgtac accggcggcg ccctcctcgg cgaccgcgtg cggatgcagg accacgcagt
      241 cgatcccggc gtgttcatcc gctcgatggc gacgaggggg cacctcgggg ggatggctct
      301 ggctgccccc gaggccgtgc ggatcctcga cgcggccggc tacgacgtga tcttcgtgga
      361 gacggtcggc gtcggacagg ccgaggtcga cgtcgcagcg gccacggaca cggcggtcgt
      421 ggtgatggcg ccggggatgg gggacgccgt ccagatggcg aaggccggca tcctcgaggc
      481 cgccgacgtc ttcgtcgtga acaaggcgga tcgcgacggc gcccccgacg tggtcaagga
      541 gctccgccag atgctgcaca tgggtgccga acgcgactgg gacccgccgg tgctgatggc
      601 caccgcggcg aaggccgaag gggtcgacga agcgtgggcg gcgatccgcg agcaccgtga
      661 cggcgccgag gcgtccggcg cgctcgccgc gaagcggcga gcccgcctcc tccgtgaggc
      721 ggagagcctc gccgccgagc ggttccgggt caaggcggcc gaagccctcg agcaggaccc
      781 tgcgctcggt gacgacctcg ccgagcgccg catcgacccg tacgccgccg cccaggagct
      841 cctccgccgc gccaaggggc tcgatcaact ggcgtgatcc gagacggaag cgaacccctt
      901 gcgttgaaac ctgacgccga gcatcggccg ccggtcggcc ctagtgtccg cggccgcccg
      961 agccggcccg gaagagggcg atccagtcgc cgaccgtgag gtcgatggca cgatcgtccg
     1021 gatcgaggtc ggcggcccgg atcactgacc ttcggagccc gagggagcgg tcgatcggca
     1081 caccagcctt ctcgaatcct cgtcgcacga gccgttcgaa cgaggtgcgc tcgatcggtg
     1141 cgatcagtgg ccgcgggcga cgctctccga cgagccaagc ggcgtcgacg gaaggtgccg
     1201 ggtggaacga ccgcgccggg atccgtcggc gaacgccgaa gcgccactcg gtgccccaga
     1261 gcacggagag cacacttcct ctggcggcgg ctcgtttgcg ggcggtctcg agctgcgtga
     1321 tcaggtcgag gcgttcgacc tgatcgagcc gagtgatccg ccggaggaga gcggtggtga
     1381 tatcgaacgg gatgttcccg acgactcgga gcgaggccca gggatctgcg tatcgagggc
     1441 gtcacctcgg tgcacgaaga cattgggcca cgaccgcgcg gagcgaagca gcgtcgagga
     1501 caacgcggga tcgatctcga tcgcgaggac gagcccggcg aacctcgcca gttcccgtgt
     1561 cagtcgaccg gtccccgctc cgacctcgac gacgaggtcc gcggatccga cctcgaacgc
     1621 ccggacgagt tcagccgcgg atcgcgggcc gaggaagtgc cggcccctcg tagggacgtc
     1681 gcggcgcgga cgcgaagaac gtgcgcccac ggagctcacc acacaaccca tcagaagcaa
     1741 ctcctgcgac cgaatctacg gtccgtgctt ccggtcgtcg tccatggcgg gagctgggct
     1801 tccggtcaca gccactatcc tccttagggt gagtcccgaa cggccccgcg ctccgtggag
     1861 cgagcgttcg caccgatcct tgtccggtga gcctgtcccg gcgatcgtcg ggccggacga
     1921 cctcgaaggg tgggagcctg acgagaagct cggcgaaccg ggcgccttcc ccttcacccg
     1981 gggcgcctac ccctcgatgt accggggcag gctctggacg atgcgtcagt tcgccgggta
     2041 cggcacgccg gcggagacga acgcccgcta ccgcttcctg accgaccacg gccagaccgg
     2101 actctcggtc gcgttcgaca tgccgacgct catggggctg gactccgacg atcctcggag
     2161 cgagggcgag gtcggccggt gtggcgtcgc cacggattcg ctcgacgact tcgagacgtt
     2221 gttcgaccag atcccgctcg gggatgtcac gacgtcgatg acgatcagcg gtccggcggt
     2281 cgtcgtgttc gcgttcttcc tcgcgaccgc cgaggagcaa ggcgtcgact ggtcgaccct
     2341 cgacggcacg ctccagaccg acatcttcaa ggagtacatc gcgcagaagg agtgggtgtt
     2401 cccgccgcgg ccgcacctgc ggctgatcgg cgatctgatg gcgttctgcg ccgagcgcgt
     2461 cccgaagtgg catccgatca gcgtttccgg ctaccacatc cgcgaggcgg gggcgacggc
     2521 cgccgaggag ctcgcgttca cgctggcgga cgggttcgcc tacgtcgagc tcgggatgca
     2581 gcggcgcctc gacgtcgaag ccttcgcgcc cgcgctctcg ttcttcttca acgcgcacat
     2641 cgatttcttc gaggagatcg cgaagctccg cgccgcccgc cggatctggg cgcgctggat
     2701 gcgcgatcgc tacggcgcga cgagccccga ggcgatgcgc ctgcggttcc acacgcagac
     2761 ggcgggcgtg tccctcaccg cgcagcaacc gatgaacaac gtcgtgcgca ccgcgatcga
     2821 ggcgctcgcc gcggtgctcg gcggcaccca gtcgttgcac acgaacgcgc tcgacgaggt
     2881 gctcgcgctc cccaccgagg acacggcgaa gctggcgctg cggacccagc aggtgatcgc
     2941 cttcgagtcg ggggtccccg acgtgatcga cccgctcggc ggttcgtggt tcgtggaaga
     3001 tgcgacggac cggctcgagg ctcgggccga ggagatcttc acggagatcg agcggatggg
     3061 tgagggcgcg atgctcgagg gcgtcctcga ggggatcgag caagggtggt tccagcgacg
     3121 gatcgccgag tccgcgttcc aggagcagaa acggtacgag tcgggagatc tcgtgaaggt
     3181 cggcgtgacg gactacgtgg acgcgaacga gaacccgatc gacacgctcg tgatcgggga
     3241 ggaggcggag cgcacccagc tcgcgagcgt tcgccggacc cgtgccgcgc gtgacgcggc
     3301 cgccgcggac gacgcgctga tcgccctgaa ggaggcaacc accaccgacg cgaacctgat
     3361 ggagccgctg atcgactgtg cccgcgcccg atgcaccgaa ggggagatcg tgggggctct
     3421 cgcggcggtc ttcggcacct accgggagac gcccgctttc tagcgcggcg ccagttgcaa
     3481 gctattgcaa gaacgaagta ggctgtctgc atggatatgc gacgaggtgc gctcgcggcc
     3541 gccctcctcc tgctcctcac cggcgcctgc gggtcgggtg gacctggttc gggggcgggc
     3601 gtccaggtcg tcacgaccgt ctcgccgatc acgaacatcg tgcagaacat cgggggcgac
     3661 cgcgtctccg tcaccgggat cgtgcccgaa ggggtcaaca gccacacgtt cgagcccgcc
     3721 ccctcggacg ccgcggtgat ggaggacgcc gacatcgtct tcatcaacgg cctgcacctg
     3781 gaggagccga cgcgcgagct cgcagaggcg aacgtcggcg acggggtccc catcgtgcag
     3841 ctcgcggacc gcacgatctc ggaggacgag tacatcttcg acttctcctt cccacgatcc
     3901 gaaggcttcc cgaaccccca cctctggacg aacccgccct acgcgaagga cttcgccgag
     3961 atcgtccgtg atgagctctc cgagctcgat cccgagggtt cgtccacctt cggagagaac
     4021 ttcgaggcgt tcgcggaccg ggtcgacgcc ttggatgccg ccgtccgctc ggccaccgag
     4081 acggtggcgg aagaggaccg caagctcctc acgtatcacg actcgttccc gtacttcgcc
     4141 cgcgagtacg ggtgggaggt cgtgggggcg atccaaccgt cggacttcgc cgagccgaca
     4201 ccgcaggaga tcgtgggact gatcgagcag atccgcgccg agagtcttcc cgcgatcttc
     4261 ggctccgagg tgttcccgag cccggtcctc gagcagatcg cggcggagac gggcgcgacc
     4321 tacgtcgacg acctgcgcga cgacgatctc ccgggggagg ccggcgatcc ggaccacagc
     4381 tacctcggat tgatggtgtt cgacttcacc acgatcgtcc gcgcgttggg cggggacccg
     4441 gcagcgttcg agggcctcga cgtcacgaac ctcgcgccgg acgcggccga agggacccgc
     4501 tactgatgga cgagctcgta cggttcgacc gtgtcacctg ccgctacggc cgtgacccgg
     4561 tgctcgtgaa cgtcgacctg gagatcgggg cgggcgagtt catcggcgtc gtcgggccct
     4621 ccgggagtgg gaagagcacg ctgttgaagg cgatcgccgg atcggtcgaa ccgatcgccg
     4681 ggcaggtccg acgcgatccc gagctgtcga tcggctacgt gccgcaggtc gaaacggtga
     4741 actggtactt cccggtcacc gttcgcgaag cggtcctcat ggcgaagatc gacgggcgcc
     4801 gtgtcccttg gccctcgaag caggagtcct cgcaggcgca ggaagtcctc gagcggctcg
     4861 gattgggagg gttgtccgac cgacacatcc gcgagctctc ggggggccag cagcagcggg
     4921 tcttcatcgc ccgcgcgatg ctcgggcgcc cgcgtctgct gctgctggac gagcccacga
     4981 gcggcgtcga cgtccgaacc cgccacgacg tcatgcacgt gatccacgat ctccatcacg
     5041 agggactcgc catcgtcctc accacccatg acctgaacgg catcgcgtcc cacctgcctc
     5101 ggctcgtctg cctgaaccgc gaggtgatcg ccacggggac gccgcgacag gtgatcacca
     5161 gcgaggtgct ggagaggacc tacggcgccc cgctcgaggt gctctcgcac ggcgggatgc
     5221 ccgtcgtgct cgagcagtac cccgagctcg tcgaggagga ggagggcgct cgcgtgctgc
     5281 cgctccggga ggaccatcat gagcaccctg cttgagccgt tcggctacga gttcttccaa
     5341 cgagcgctgc tcgtcgccac gatcaccggc gcgctctgcg gtctgatcgg cgtgtacgtg
     5401 gtgctccgag ggatgagcta catcggccac gggctctcgc acgcgatctt cggcggcgcg
     5461 gtggcgagct acatcgtcgg cgtgaacgtc ttgcttgggg cggggctctg ggggctggtc
     5521 gcggcgctct tcatcaacaa ggtcgcgcgc cggcgatcga tcggggccga cgcggcgatc
     5581 ggtgcgatca cctcggcgag cttcgcgatc gggatcatcc tgatctcgac ccaacggacg
     5641 ttcgtcgtga acttcgaggc cctcctgtgg gggaacgtgc tcggcgtcac cggggtcgat
     5701 gtgctcgtcg tgctggccgt cgcggcgggc tcgatcctgt tcgtgttcct cggctaccgg
     5761 cagctgctct tctcgacgtt cgatcccgag gtggcggaag tctcgggcgt gcggacggcg
     5821 cgtgtcgaca tcgggctggc ggtgatgctc acggcgacga tcgcggcgac gatgaacgtg
     5881 atcggtgtga cgatgatcgc cgcgatgctc gtgatcccgc ccgtgatcg
//
LOCUS       DASUVE010000226         3225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_484725, whole genome shotgun sequence.
ACCESSION   DASUVE010000226 DASUVE010000000
VERSION     DASUVE010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484725"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VF036_04900"
     CDS             complement(<1..673)
                     /locus_tag="VF036_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6264600.1"
                     /translation="MADLDAARRELEEALGPDEVLSDPLALRLYARDASMVEGAAGLV
                     VFPRSTDDVAACVRAAAMHDLAVVPRGSGTGLAGASTPIGDALVVVTSKMDRILEIRP
                     EDRLAWVEPGLFNLDLGVALRGTGFTYMPDPSSQQVSSIGGNVGTNAGGPHCLAYGVT
                     SAHVLALDVVLGDGTVTRLGSEGPEAAGYDLRGVVVGSEGTVGLVAAVCVRLTPEPPA
                     ITTMLL"
     gene            693..1169
                     /locus_tag="VF036_04905"
     CDS             693..1169
                     /locus_tag="VF036_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015032875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264601.1"
                     /translation="MATWAEFELAEPELAAFGKERLDGQVCFHATLRTDGSPRVHPVE
                     PWIAVGLLLVRFRGHSPKVDEVLRDGRYALHSPMDNPDGVGGEFMVRGWMEQIGDTHP
                     AVERFVHDASYPLAFYAMSVEEAVGTTYEGEGAEPVYRRWRSQATRPGEPSGAVKS"
     gene            complement(1124..1459)
                     /locus_tag="VF036_04910"
     CDS             complement(1124..1459)
                     /locus_tag="VF036_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006138536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6264602.1"
                     /translation="MADLSTVEGVHESLDDIQLVDCREQYEWDAGRIAGALHIPLNDV
                     MAGVGADVLDLAKPVAVICRSGNRSELASMMLQARGYDATNVEGGMEAWAAAGYDFTA
                     PDGSPGRVA"
     gene            complement(1494..2006)
                     /locus_tag="VF036_04915"
     CDS             complement(1494..2006)
                     /locus_tag="VF036_04915"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006370999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX6264603.1"
                     /translation="MPRIVLVEGDITEQRVDAIVNAANATLLGGGGVDGAIHGRGGPS
                     ILEACRRIRATEWPDGLPTGRAVATTGGDLPARWVIHTVGPVYAREEDPATLLSSCHT
                     ESLRVADELGAALVAFPAISTGVYGYPVDGAALVAVEAVRTAETSVREVRFVLFDGTA
                     HAAFERALTG"
     gene            2024..2962
                     /locus_tag="VF036_04920"
     CDS             2024..2962
                     /locus_tag="VF036_04920"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEX6264604.1"
                     /translation="MIVCLAANPSVDRLFEVERLVKGDIHRPSGFMQVAGGKGLNVAR
                     AARSLGADVCAVALLRGHTGRWLQESLAAEGVEGEFVWTHGESRSSLSVADRETAGLT
                     EFYEHGAVAPDAAWVELTHAAATRWERGCWLTISGSLPRGAPDEGYRDLIEEARRGGV
                     RVALDSEGERLRLALTAEPDIVKVNVAEAGELLGVPTARRDDSLAAATKIRNMAGGDG
                     HAGIVTRGAEGVLLAAPDGTLYEGVLYVRGRYPVGSGDAFLAGLITALERGSDWEEAL
                     RVALGAGAANAELAGAARIDPSRAAALARQADVHLL"
     gene            3030..>3225
                     /locus_tag="VF036_04925"
     CDS             3030..>3225
                     /locus_tag="VF036_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264605.1"
                     /translation="MLDSPDAFGSTYGEEASHSESAWRDWAAGRWRGGTAIALAGRDP
                     TGAVVGTATGAEFAAEPGVAH"
BASE COUNT          463 a         1110 c         1192 g          460 t
ORIGIN      
        1 ccagcagcat cgtcgtgatc gccggcggct ccggggtgag gcgcacacag acggccgcga
       61 cgagtccgac cgtcccctcg gagccgacca ccaccccacg gaggtcgtag ccggccgcct
      121 ccggtccctc ggagccgagg cgagtcacgg tcccgtcccc gagcaccacg tcgagcgcca
      181 ggacgtgcgc ggacgtgacg ccgtacgcca ggcagtgcgg gccacccgcg ttcgtcccca
      241 cgttcccccc gatcgacgac acctgttgcg aggacgggtc cggcatgtac gtgaatcccg
      301 tacctcggag ggcgacgcca agatcgaggt tgaacagacc gggctcgacc cacgccagtc
      361 ggtcctcggg cctgatctcg aggatgcggt ccatcttcga cgtgacgacc acgagggcgt
      421 cgccgatcgg tgtggacgcg cccgcgagcc cggtcccact ccctcgaggg accaccgcca
      481 ggtcgtgcat cgcggccgcg cggacgcacg cggcgacgtc gtcggtggaa cgagggaaga
      541 ccacgagacc ggccgcgccc tcgaccatcg acgcatcgcg tgcgtagagg cggagcgcca
      601 aggggtccga cagcacctcg tcgggaccga gcgcttcctc gagctcccgt cgcgccgcgt
      661 ccagatccgc cacgtcgtta gggtaggcgg ggatggcgac ctgggccgag ttcgagctcg
      721 ccgagccgga gctggccgcg ttcgggaagg aacggctcga cgggcaggtc tgcttccacg
      781 cgacgctccg caccgatgga tcgcctcggg ttcatccggt ggagccgtgg atcgccgtcg
      841 gtctcctgct cgttcggttc cgaggccaca gccccaaggt cgacgaggtc ctccgcgacg
      901 gtcgctacgc gctccactcg ccgatggaca acccggacgg cgtgggcggc gagttcatgg
      961 tgcggggctg gatggagcag atcggtgaca cgcatccggc cgtagagcgg ttcgtccacg
     1021 atgcgtcgta tccgctcgcg ttctacgcga tgtccgtgga ggaggccgtg gggaccacct
     1081 acgaggggga gggagccgag cccgtctacc ggcggtggcg ttctcaggcc acgcggccgg
     1141 gggagccgtc gggcgcggtg aagtcgtagc ccgcagcggc ccaggcctcc atcccaccct
     1201 cgacgttggt ggcgtcgtag ccccgcgcct gcagcatcat cgaggcgagc tcgctgcggt
     1261 tgccgctccg gcagatgacg gcgacgggct tcgcgaggtc gaggacgtcg gctccgaccc
     1321 cggccatcac gtcgttcagg gggatgtgga gggcgcccgc gatcctgccc gcgtcccact
     1381 cgtactgctc gcgacagtcc accagctgga tgtcgtcgag cgactcgtgg acaccctcga
     1441 ccgtgctgag atctgccatc ggaccgatgc tagcagtggg tccggcgaaa ggttcacccg
     1501 gtcaacgcac gttcgaacgc cgcatgcgcg gtcccgtcga acagcacgaa gcgcacctcc
     1561 cgcacggacg tctcggccgt tcgcaccgcc tccaccgcga cgagcgcagc cccgtcgacc
     1621 gggtagccgt agaccccggt cgagatcgcc gggaacgcga cgagggcggc cccgagctcg
     1681 tcggcgacgc ggagcgactc cgtgtggcag ctcgacaaga gcgttgcggg atcctcctcg
     1741 cgcgcgtaga ccggcccgac cgtgtggatc acccaccgag ccgggagatc gccgccggtc
     1801 gtcgcgaccg cacgaccggt tgggagaccg tccggccact cggtcgcgcg gatccgtcgg
     1861 catgcctcga ggatcgaggg gccgccgcgg ccatggatcg cgccgtcgac accgccgccg
     1921 ccgagcaagg tcgcgttcgc cgcgttcacg atggcgtcga cccgttgctc cgtgatatcg
     1981 ccctcgacca gaacgatgcg gggcatgcgt ctatcgtagg tcgatgatcg tctgtctcgc
     2041 cgcgaacccg tccgtcgatc ggctgttcga ggtcgaacga ttggtcaagg gcgatatcca
     2101 ccggccgtcc gggttcatgc aggtcgcggg cggcaagggg ttgaacgtcg cacgggcggc
     2161 gcggtcgctc ggcgccgacg tgtgtgcggt cgctctgctc cgaggacaca cgggtcggtg
     2221 gttgcaggaa tcgctcgccg ccgagggtgt ggaaggcgag ttcgtatgga cgcacgggga
     2281 gagccggtcc tcgctctcgg tcgccgaccg cgagaccgcc ggactgaccg agttctatga
     2341 gcacggcgcc gtcgcaccgg acgccgcgtg ggtcgagctc acgcacgccg ccgcgacgcg
     2401 gtgggaacgc ggttgctggc tcacgatctc gggctccttg ccgcggggcg cgcccgacga
     2461 gggctatcgg gacctgatcg aggaagcgcg ccgcggcggt gtccgcgtcg cgttggattc
     2521 cgaaggggag cgcctgcgcc tcgccctcac cgcggagccg gacatcgtga aggtgaacgt
     2581 cgcggaggcg ggagagctcc tcggcgtgcc gacggcgcgc cgggacgact ccctcgcagc
     2641 tgctaccaag atccgcaaca tggcgggtgg cgacggccac gccggcatcg tcacgcgagg
     2701 agcggagggc gtgttgctgg cggcgccgga cggcacgctc tacgaagggg tcctgtacgt
     2761 gcgcggccgc tatcccgtgg gaagcggcga cgcgttcctc gccggactga tcaccgcgct
     2821 cgagcggggg agcgattggg aggaggcgct ccgagtcgcg ctcggggcgg gagcggccaa
     2881 cgccgagctc gcgggcgctg cccggatcga tccgtcgcgc gccgcggccc tcgcgaggca
     2941 ggcggacgtc catctcctct agcccggtcc gggtcgagcc gttcgtcgag gacgactggg
     3001 tcgcgttccg tgagatccgg ctccggtcgt tgctcgacag tcccgacgcg ttcggctcga
     3061 cctacggcga ggaagcctcg cactcggagt ccgcgtggag ggactgggcg gcggggcgat
     3121 ggcggggtgg aacggcgatc gcgctggcgg gtcgggatcc gacaggggcg gtcgtgggga
     3181 cggcgacggg cgcggagttc gcggccgagc ccggtgtggc gcacc
//
LOCUS       DASUVE010000227         3118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_672198, whole genome shotgun sequence.
ACCESSION   DASUVE010000227 DASUVE010000000
VERSION     DASUVE010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672198"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1559
                     /gene="thrS"
                     /locus_tag="VF036_04930"
     CDS             <1..1559
                     /gene="thrS"
                     /locus_tag="VF036_04930"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX6264606.1"
                     /translation="QPFLREEVTRADALARREGQSFKREIIESIGSAGGEVATGDVVS
                     LYRNDGWVDLCEGPHVPSTGRLGAFTLTSIAGAYWRGLETNPMLTRIYGTAWATHGDL
                     EAHLSRLEEAEKRDHRRLGRELDLFSSPDELGPGLWIWHPRGGIFRKQLEDWVRDLHV
                     ERGYDLIVTPHIARSVLWETSGHLEKYRENMYPPMEADTGDYYVKPMNCPFHVLVYKS
                     QVRSYRDLPMRLSELGTIYRHEKAGTLHGLLRIRGGTQDDSHIICTPEQLIDEILAVF
                     DLTLEIHRTFGFTDPVVELSTLPIAQSIVDAETAERATEALTLALEKSGLEYRVAEGE
                     GAFYGPKVDFHFRDALGRLWQLTTVQCDFALPERFDMEYTGDDNQRHRPVMIHRAILG
                     TLERFSAVLIEHYGGAFPLWLAPEQLRLVPVADRHVPNCMELAVTARASGLRVHVDET
                     KETVGKKIRAAQLMKAPYVVVVGDRDLDAGTFTVRDRSGEEHQGVPFDGIVSTLAEEA
                     RSRSLDQSTF"
     gene            1560..2051
                     /locus_tag="VF036_04935"
     CDS             1560..2051
                     /locus_tag="VF036_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017595163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX6264607.1"
                     /translation="MDRLWSPWRMEYIQATKDEPPDAPCIFCRIGDGEESGRVLARED
                     LAYVVLNKYPYSPGHVLVIPSRHVGDLGDVVEDENLALQRLLQRSVKALREEFAPHGF
                     NIGMNLGRVAGAGVPDHLHWHVVPRWSADTSFMPVVGQTLVLPELVEETAKRLAPRFL
                     APE"
     gene            2126..2782
                     /locus_tag="VF036_04940"
     CDS             2126..2782
                     /locus_tag="VF036_04940"
                     /inference="COORDINATES: protein motif:HMM:NF027589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEX6264608.1"
                     /translation="MENLEHVVAVLGDVVGSRGHPDPERRRGSVADALALANDRLAPL
                     QPLTPTIGDEFQGLFADLVSALEATLIVRLALRGGVDVRFGIGSGPLTRFEESRAPFD
                     QDGPAWWSARAAVDHVRELSQRQESPRGLRTRWVDAQDERSFRVASVNAFLHCRDELV
                     GAMDARDAAAMLGLLADEPLTRIAETEGISPSALSQRAIRGGLYAIRRAHEGLREVLA
                     "
     gene            2779..>3118
                     /locus_tag="VF036_04945"
     CDS             2779..>3118
                     /locus_tag="VF036_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264609.1"
                     /translation="MIAVGIWLVSIGLSDLVAGLSGEPKAGTRTIGAIITGFAAPFVL
                     GPASGATTPTLLILTLLTGVTIAVWIVLRRDPSRMTALRALGALLFVLTAFVAVSLFG
                     AEPVSSFRTEP"
BASE COUNT          483 a         1021 c         1092 g          522 t
ORIGIN      
        1 atcagccgtt cctccgcgag gaggtcaccc gagcggacgc gctcgcccgg cgcgagggcc
       61 agtcgttcaa gcgggagatc atcgagtcga tcggttcggc cggaggcgag gtggcgaccg
      121 gcgacgtcgt gtcgctgtac cgcaacgacg ggtgggtgga cctgtgcgag ggcccccacg
      181 tcccctcgac cggccgcctc ggcgcgttca cgttgacctc gatcgccggc gcgtactggc
      241 gggggctcga gacaaacccg atgctcaccc ggatctacgg cacggcctgg gcgacacacg
      301 gggacctcga ggcgcacctg tcccggttgg aggaagccga gaagcgcgac caccggcgcc
      361 tgggccgcga gttggatctg ttctcgagcc ccgacgagct cggtccgggc ttgtggatct
      421 ggcatccccg cggtggcatc ttccggaagc agctcgagga ctgggtccgg gacctccacg
      481 tcgagcgcgg gtacgacctc atcgtcacgc cgcacatcgc ccggtccgtg ctgtgggaga
      541 cgagcggcca tctcgagaag taccgggaga acatgtatcc ccccatggag gcggacaccg
      601 gcgactacta cgtgaagccg atgaactgcc cgttccacgt gctcgtctac aagtcgcagg
      661 tgcggagcta ccgagacctt ccgatgcggc tgtcggagct cggcacgatc tatcgccacg
      721 agaaggccgg cacgcttcac gggctgctgc ggatccgtgg ggggacccag gacgactcgc
      781 acatcatctg cacccccgag cagctgatcg acgagatcct cgctgtcttc gacctgacgc
      841 tcgagatcca ccggacgttc ggcttcaccg accccgtggt cgagctctcg acgctgccga
      901 tcgctcagtc gatcgtggac gccgagacgg ccgaacgcgc caccgaggcg ctcacgctcg
      961 cactcgagaa gagcggtctc gagtaccgcg tggccgaggg cgagggcgcg ttctacggac
     1021 cgaaggtgga cttccacttc cgcgacgcgc tcgggcgcct gtggcagctc acgaccgtcc
     1081 agtgcgactt cgccctgccc gagcgcttcg atatggagta cacgggcgac gacaaccagc
     1141 ggcaccgacc agtgatgatc catcgtgcga tcctcgggac gttggagcgg ttcagcgccg
     1201 tcctgatcga gcactacggc ggcgcgttcc cgctctggct cgcgccggag cagctccggc
     1261 tggtcccggt ggcagaccgg cacgttccca actgcatgga gctcgccgtc acggcgcggg
     1321 cgtccggcct ccgggtacac gtcgacgaga cgaaggagac cgtgggcaag aagatccgcg
     1381 cggcccagct gatgaaggcg ccgtacgtgg tggtcgtggg cgatcgagat ctggatgcgg
     1441 gaacgttcac cgtccgggac cgatcggggg aggagcatca gggcgtgccg ttcgacggga
     1501 tcgtgtcgac gctcgccgag gaggcgcgca gccgatcgct cgatcagagc accttctaga
     1561 tggatcggtt atggagcccg tggcggatgg agtacatcca ggccacgaag gacgaaccgc
     1621 ccgacgctcc ctgcatcttc tgccggatcg gcgacggcga ggagtcgggg cgtgtcctgg
     1681 cccgggagga cctcgcctac gtcgtgctga acaagtaccc ctacagcccg ggccacgtcc
     1741 tggtgatccc gtcgcggcac gtcggagacc tcggggacgt cgttgaggac gagaatctgg
     1801 cgctccagcg gctcctccag cgttccgtga aggccttgag ggaggagttc gccccgcacg
     1861 gcttcaacat cgggatgaac ctggggcgcg tggccggcgc cggcgttccc gaccatctgc
     1921 actggcacgt cgtgcctcgg tggagcgcgg acacgagctt catgccggtc gttggccaga
     1981 cgctcgtgct tcctgagctg gtcgaggaga cggcgaagcg gctcgctccg cgattcttgg
     2041 ctccagaatg aagtccatgc ctgtaaaccc ccagaatgaa ggcaatacct ttacatgtct
     2101 actgtgaagt catatccttc atgtcgtgga gaacctggaa catgtcgtcg cggtgctggg
     2161 agacgtcgtc gggtcgcgag ggcaccccga ccctgaacga cgccgaggct ctgtcgccga
     2221 cgccctcgcg ctcgcgaacg accggctcgc gccgctgcag ccgctgaccc ctacgatcgg
     2281 cgacgagttc cagggactct tcgccgacct cgtgtcggcg ctggaagcga cgctgatcgt
     2341 ccggttggcc ttgcggggag gggtggacgt gcgtttcggg atcggatcgg gacctttgac
     2401 caggttcgag gagtcgcgcg cgcccttcga tcaggatgga cctgcgtggt ggtccgccag
     2461 ggcggcggtc gaccacgttc gtgagctgtc gcagcggcag gagtcgccga gagggctccg
     2521 gacccggtgg gtcgacgctc aggatgagcg gtcgttccga gtggcttccg tcaacgcgtt
     2581 cctgcattgt cgcgacgagc ttgtcggcgc gatggacgcc cgcgacgcgg cggcgatgct
     2641 cgggctgctc gcggacgagc cgttgacgag gatcgcggag acggaaggta tcagcccctc
     2701 cgcgctctcg cagcgggcga tccgaggcgg cctctacgcg atccgtcgtg cgcatgaggg
     2761 gctacgggag gtgctcgcgt gatcgccgtg gggatctggc tcgtatcgat cgggttgtcg
     2821 gatctcgtgg ccggcctctc cggcgagccg aaggccggta cgaggacgat cggcgcgatc
     2881 atcaccggct tcgctgctcc gttcgtcctc ggtcccgcga gcggcgcgac gaccccgacg
     2941 ctcctcatcc tcacgctcct gaccggcgtc acgatcgctg tgtggatcgt gctgcggcgg
     3001 gacccgtcgc ggatgacggc gctccgagcg ctcggcgcgc tgttgttcgt cctgacagcg
     3061 ttcgtggcgg tctcgctgtt cggggcggag ccggtctcca gcttccggac cgagccga
//
LOCUS       DASUVE010000228         3886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_295637, whole genome shotgun sequence.
ACCESSION   DASUVE010000228 DASUVE010000000
VERSION     DASUVE010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295637"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VF036_04950"
     CDS             complement(<1..859)
                     /locus_tag="VF036_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264610.1"
                     /translation="MSDVPGGPTPPTPPPPPPMMGGPQGSIPPKTVGDILSAAFNIYK
                     ANASQLILVVAVVVVPLSLIGALFSEVVFEPEQIRVTDAGGVVVLDSAGRGLGVLLLV
                     GAIGALIAVLISAILQAALLRAATQATIGDPVDVEASYRYGLKRLWSVILVSLLVALI
                     VAAGFVLLVIPGIIFLVFLSLSIPVLIVEGRRGTEALGRSWELVKGHFWHALGVIVVA
                     ALIVGIISGIIGGIGGDNWAVRWIFTAIAQILTAPFASLVSILLYLDLRARSETLFAE
                     TLRRELASGT"
     gene            993..1364
                     /locus_tag="VF036_04955"
     CDS             993..1364
                     /locus_tag="VF036_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014691291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6264611.1"
                     /translation="MSSLVLNVTFDCQDAGAVARFWEAVTGHPRELVQQPGNDHWVVS
                     PRDGSLPRLVFVTVPEEKSIKNRVHLDLLPNDSSQEDEVARMLDLGARVVDDRRRSDP
                     GGWVVMADPEGNEFCVEHPRS"
     gene            1421..2599
                     /locus_tag="VF036_04960"
     CDS             1421..2599
                     /locus_tag="VF036_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6264612.1"
                     /translation="MDLRLSAADLDLQQKARDFSDQVLVPLEDECEANDGLTPESHAA
                     AKQAVLDWEFHAINHTPENGGRGYDLFEQMLIEEQWGRATGALWDIPWRPSIPLAAGT
                     EEQKERFLRPACRGERRDAYAITEEGAGSDPTMVETSAKRDGDTWTITGEKWHVTSGD
                     VADFFLVHTHVEGDPAKATIFLVDKDAPGVRLVRTPKYMHTFVFEHPIFAFEAVRLGD
                     DRILGEVGRGFELTKDWFVEERLMIGARTTGAATRALELSLDFARDRRQFGRPIVDFE
                     AVEFMLADMAAELMAAKSMLYRVCWQAGRGGADRKELHAMAAAVKLVCSETAGRVADK
                     AVQIHGGRGYMREHPVERLWRELRVDRIWEGTSEIQRVVIGNELRKRGAGRYTGWPAE
                     "
     gene            complement(2600..2965)
                     /locus_tag="VF036_04965"
     CDS             complement(2600..2965)
                     /locus_tag="VF036_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264613.1"
                     /translation="MAGVFRSSGRRTNPLGAISVVTGLASALASAVLWIYRIQPDSRI
                     LGQYSAEVAAGGQLADQLVVLAAILGVMAVLSSIMTSSSGEGGGSYFMGLVLGLVGLS
                     YPLLDWLNVMSEPLRPSIL"
     gene            complement(2965..3615)
                     /locus_tag="VF036_04970"
     CDS             complement(2965..3615)
                     /locus_tag="VF036_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264614.1"
                     /translation="MTKRMIAATLLRFLCACDGEPELFPQPSSPTSASPFPSTPTLSS
                     PTPPALPSDPLPTSGEASETCVEGWVSPGADSALFDTPLRIIRRSVQLPSEPTVVEMR
                     YFTGPESPPSDKGYLLVVERWYVKLYTEDDLRFQGRFLVESRDFGDGVAAVAPYDTEG
                     FRSPDWSGFQWADTEPSPYPGLPGEWSGTRYDFVKGGDGLDIPGLPTEVRGCLDTA"
     gene            3671..>3886
                     /locus_tag="VF036_04975"
     CDS             3671..>3886
                     /locus_tag="VF036_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX6264615.1"
                     /translation="MDTGPYGETRDERHPMPLSRRRFLRTSAMAVAGGVLFSCTGGTR
                     TPKVDDTIPETDTRWPIKRVIYVMLENR"
BASE COUNT          711 a         1292 c         1326 g          557 t
ORIGIN      
        1 aggtgcccga agccagctcc cgccgcaggg tctctgcgaa cagcgtctcg ctccgcgcgc
       61 ggaggtccag gtagagaagg atcgagacga gcgacgcgaa cggagccgtc aggatctgtg
      121 cgatggcggt gaagatccat cgcacggccc agttgtcgcc gccgatcccc ccgatgatcc
      181 ctgagatgat cccgacgatc aacgctgcga ccacgatcac gccgagcgca tgccagaagt
      241 gtcccttcac cagctcccac gatcgtccca gcgcctcggt gccccgtcgg ccctcgacga
      301 tcagcaccgg gatcgacagc gataggaaga cgaggaagat gatccctggg atcaccaaca
      361 gcacgaagcc cgcggcgacg atcagggcca caagaagcga cacgaggatc acgctccaca
      421 accgcttgag cccgtatcgg tagctcgcct cgacatcgac cgggtcaccg atcgtcgcct
      481 gcgtggcggc tcgcaggagg gcagcctgca ggatggccga gatgagcacc gcgatcaaag
      541 cgccgatcgc cccaacgagg agcaggacac cgaggccacg cccagcggag tcgagcacga
      601 ccacgcctcc cgcgtcggtc acccggatct gctcgggctc gaacacgacc tcggagaaca
      661 gagcgccgat cagcgacaac gggacgacca cgacggcgac gacgaggatc agctgcgacg
      721 cgttcgcctt atagatgttg aaggctgcgg acaggatgtc gcccaccgtc ttggggggga
      781 tgctgccctg gggtccgccc atcatcggcg gcggtggagg aggagtcggc ggcgttggcc
      841 cgccagggac atcgctcatg catggtcccc tttccggtcg tgaagctgaa gcctagcctg
      901 tcgatgggct gtgagtgccg atcacgtcac gcgctccagc gcgacggagc actcttgtca
      961 cgaccatccg ctccttggca cgctcagatg tcatgagttc tctcgttctc aacgtcacgt
     1021 tcgactgcca agatgcgggc gccgtcgccc gcttctggga ggccgtcacc ggacatccca
     1081 gggagctggt gcagcaaccg ggaaacgatc actgggtggt gagccctcgc gatggaagct
     1141 taccgcggct ggtgttcgtc accgtgcccg aggagaagtc gatcaagaac cgggtgcacc
     1201 tggacctgct gccgaacgac tcgagccagg aagacgaggt cgcccgcatg ctcgacctgg
     1261 gcgcccgcgt cgtcgacgat cgtcgccgct ccgaccccgg gggctgggtc gtgatggcgg
     1321 atcccgaggg caacgagttc tgcgtcgagc atccacggag ctgaagctcg gcggctcggt
     1381 tcacccctcg ttggacgcgc cgcctcctac actccgccgg atggatcttc gactcagcgc
     1441 agcggatctc gacctccaac agaaggcgcg tgacttcagc gaccaggtcc tcgtcccgtt
     1501 ggaggacgag tgcgaggcca acgatggact cacgcccgag agccacgcgg ccgcgaagca
     1561 ggccgtcctc gactgggagt tccacgcgat caaccacacg cccgagaacg gtggccgcgg
     1621 atacgatctg ttcgagcaga tgctgatcga ggagcaatgg ggccgcgcta ccggagccct
     1681 ctgggacatc ccctggaggc cctcgatccc actcgcggcc ggcaccgagg agcagaagga
     1741 gcgcttcctc cggcccgcat gccgcggcga gcgacgggac gcctatgcga tcaccgagga
     1801 aggcgccggc tccgacccga cgatggtgga gacgtccgcc aagcgcgatg gcgatacgtg
     1861 gacgatcacg ggcgagaaat ggcacgtgac ctcgggtgac gtggcggact tcttcctcgt
     1921 ccacacccac gtcgaggggg atcccgcgaa ggcgacgatc ttcctcgtcg acaaggacgc
     1981 gcccggcgtg cgcctcgtcc gcacgccgaa gtacatgcac acgttcgtct tcgagcaccc
     2041 gatcttcgcg ttcgaggccg tgcggctggg cgacgaccgg atcctcggcg aggtcggccg
     2101 cggcttcgag ctcacgaagg actggttcgt cgaggaacgc ctgatgatcg gcgcgcgcac
     2161 gacgggtgcc gccacgcgcg cgctcgagct ttccctggac ttcgcgaggg accgccgtca
     2221 gttcggccgg ccgatcgtcg acttcgaggc ggtggagttc atgctcgccg acatggccgc
     2281 cgagttgatg gccgccaagt cgatgctcta ccgggtctgc tggcaggcgg gacggggcgg
     2341 cgccgatcgc aaagagctgc acgcgatggc ggccgccgtg aagctcgtct gcagcgagac
     2401 ggcgggtcga gtggcggaca aggccgtgca gatccacggg ggccgcggct acatgcgcga
     2461 gcatcccgtg gagcggctct ggcgtgagct ccgcgtcgac cggatctggg aggggacgtc
     2521 ggagatccag cgcgtggtga tcggcaacga gctccgcaag cggggcgccg gtcgctacac
     2581 gggctggccg gcggagtgac tacaggatcg atggacggag cggctccgac atgacgttga
     2641 gccagtccag cagcgggtag gagagcccga cgagcccgag gacgagcccc atgaagtagc
     2701 tgccgccgcc ctccccgctg ctcgacgtca tgatcgatga gaggacggcc atcacaccga
     2761 ggatggcggc caggacgacg agttgatcgg ccaactggcc gcccgcagcg acctccgcgg
     2821 agtattgccc gaggatcctg ctgtccggct ggatccggta gatccaaagg acggcgctcg
     2881 cgagagccga cgccaggccg gtcacgaccg agatcgcccc caaggggttg gtacgtctgc
     2941 ccgagcttct gaagaccccc gccatcaggc ggtatccaag cacccccgga cttcggtggg
     3001 caagcctggg atgtcgaggc cgtcgccacc cttcacgaag tcgtaccgcg tgccggacca
     3061 ttcacccggc agtcctgggt acggcgacgg ctcggtgtcc gcccactgga acccgctcca
     3121 atcgggcgag cggaaccctt cggtgtcgta gggcgccaca gcggcgacac cgtcgccgaa
     3181 gtcccgcgac tcgacgagga accgcccctg gaatcgaagg tcgtcctcgg tgtacagctt
     3241 cacgtaccag cgctcgacga ccaggagata ccccttgtca gagggcggcg actcggggcc
     3301 agtgaagtac cgcatctcca cgacggtcgg ctcgctgggg agctgcacgc tcctgcggat
     3361 gatgcgcagg ggcgtatcga acagcgccga atccgcgccg ggcgagaccc agccctccac
     3421 acaggtctcc gacgcttcgc cgctggtcgg aagcggatcc gacgggagcg ctggtggtgt
     3481 gggagacgag agtgtcggcg tcgacgggaa cggggatgcc gacgtcggcg aggacggctg
     3541 cgggaacagc tccggctcgc cgtcgcacgc acagaggaac cgcagcagcg tggcagcgat
     3601 catgcgtttc gtcatgagat gacccctcgg gaaccgaccg aggataacgg gcttgataga
     3661 aaggggggcc atggacaccg gaccgtatgg ggagacgcgc gacgagcgcc acccgatgcc
     3721 gctcagccgt cggcgcttcc tgcggacgag cgccatggcc gtcgccggcg gcgtcctctt
     3781 ctcgtgcacc ggcggcaccc ggaccccgaa ggtcgatgac acgatcccgg agacggacac
     3841 ccgatggccg atcaagcgcg tcatctacgt gatgctcgag aaccgg
//
LOCUS       DASUVE010000229         8357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1613655, whole genome shotgun sequence.
ACCESSION   DASUVE010000229 DASUVE010000000
VERSION     DASUVE010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8357
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613655"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VF036_04980"
     CDS             complement(<1..269)
                     /locus_tag="VF036_04980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6264616.1"
                     /translation="MERQVVALVAAHEESQRVASTVSALHALVDEVIVVDDGSTDATS
                     SAALLAGATVLRASQRLGKGRAIEEALGRLPGSAVWLLADADLAE"
     gene            complement(269..1210)
                     /locus_tag="VF036_04985"
     CDS             complement(269..1210)
                     /locus_tag="VF036_04985"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HEX6264617.1"
                     /translation="MISWRYHVVSIVAVILAFGLGILAGTSVVGDRFARDLQLNYDEA
                     IGERDAALDMVALQQRFADALGPTLRDGVLTGEEAVVVTVAGVDGPAQIAVDELTAAG
                     ADVLATLTIDRRLTDVPDEETVSAIEEILGVTGTDPDSLEARIADALAVRLAVGPDGD
                     TDLLGALLAAGLITADRDLDEDALLGIGGAGQLVVVAAGGRSLPDRITPQTLLVPFFQ
                     RLVQLGTPTSGVGPTDDLFGLVSAIREAPGIADCASVTVDDLDLEGIGGITMIMGIEQ
                     FLVDDDPAFRPGGDYGFGSDAMMVVPGADEPPPSCRA"
     gene            complement(1207..2379)
                     /gene="steA"
                     /locus_tag="VF036_04990"
     CDS             complement(1207..2379)
                     /gene="steA"
                     /locus_tag="VF036_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006549906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HEX6264618.1"
                     /translation="MLRRRSARTLAGGRARIDRRTKELVTRIEPGDVAVIDHEDLDRV
                     AAEALVRVGVAAVVNAAPSISGRYPNLGPMILEDAGVPLVDRVGTLVLQKIREGDPVR
                     LEHDRVFTGDRLVGVGIRQDRASIREAMESAREALDDRFEGFARNTLEYMREERDLLF
                     GGAGLPELSHSLAGRHVLVVVRGTSYREDLAALRSYIRDVHPVLVGVDGGADALLDAG
                     HVPDVVIGDMDSISDRALDLSQRPRRATTEIVLHAYPDGRAPGRARLERLGIPYVEVR
                     SAGTSEDVAFLLAHEQRAELIVAVGTHGNLREFLDKGRRGMSSTFLVRLRVGEILVDA
                     KGVARLYPSRARVWDVVILVGAVLITFGLMLFASRGLRLYVDQTLDDFVDLLQAIL"
     gene            complement(2463..4169)
                     /gene="recN"
                     /locus_tag="VF036_04995"
     CDS             complement(2463..4169)
                     /gene="recN"
                     /locus_tag="VF036_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX6264619.1"
                     /translation="MSGEAVSIPTVLRELHISGLGVIADLELELAPGLNVLTGETGAG
                     KTMVTVGLLLALGERGSAALVREDARAARVQARFDAIDGAGDWAEDGEVILARSIARD
                     GRSTARIGGQLATASALAELGAGLVEHHGQGQTQRLLAPAVQTGFLDRYAGDAHVVAL
                     GTYREAYERRTGVATELGALREAARDRERELDLLAYQVHEIETVGPRDGETEELTAEE
                     GRLAHVERLMEQASAAEHALTGEEGIADDAASLASLLENAADLDPSAREVADRARGIA
                     AEIAELARDVRSYGELLLPDPERLEAVRDRVAALKQLQRKYGPTDADVVSFLAESAEQ
                     LRTLAGADDRIAELERELEAASAAARDRAAIVTEGRTRAAPALADAVRAELEELGMPG
                     AVFEARLLPLDEPGPGGAERAELWFSASPGQPARPLARAASGGELSRVLLACRSVLAD
                     LDEVGTLVFDEVDAGIGGQAGLAVGRRLARLAAGRQVLVVTHLPQIACFADHHLRVSK
                     EDGTASVELIDGPSRAQELSRMLAGLAGSESAVTHAEELLAEAERLREGSASLGRAGA
                     VT"
     gene            complement(4150..5010)
                     /locus_tag="VF036_05000"
     CDS             complement(4150..5010)
                     /locus_tag="VF036_05000"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX6264620.1"
                     /translation="MSVVAFVVHEGRASAVETAESLRRDLQAESVDTRIARGPDDVRE
                     ERPDLVVSVGGDGTFLRAARVAAEADCPVLGVKVGRLGFLTEIEPGPDAMVLIRAALD
                     GRARVEERLAVMAEPADGNPFATQWAMNEIMVEKWTRHRLVRLAVHVGDEYVTTFSAD
                     GVIVATPTGSTAYSFSARGPIVSPGVPCLVVTPIAAHMVFDRSFVLGAGQVVVLEVVG
                     DEPGVLSADGREGIELPVGSRIRILAAPSPARLVSKDDAAGFLTRVRDKFDLPGDPPG
                     TRAPDVGGGR"
     gene            complement(5007..5804)
                     /locus_tag="VF036_05005"
     CDS             complement(5007..5804)
                     /locus_tag="VF036_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX6264621.1"
                     /translation="MGRRPLDLELVRRGLAPSRAEAQEAVRTGQVTVRGAPATKSTTL
                     VSADEALVVAGPGRPFVSRGGAKLAAALDAFAIDPSGRDCLDAGASTGGFTDCLLQRG
                     ARHVVAVDVGYGQLAWGLRTDPRVTVLDRTNVRELDREVLPYLASLVTADLAFTSLRT
                     VVRRLVDVADRGADLVLLVKPQFEAPRAEVGAGGVVTDPDVWRRAIGVVADAAAEAGA
                     PAAGVIPSPITGPAGNVEFLLRATIGGPRAHLGVDVAIGAARELTNA"
     gene            complement(5812..6192)
                     /locus_tag="VF036_05010"
     CDS             complement(5812..6192)
                     /locus_tag="VF036_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264622.1"
                     /translation="MVLDDVRKALESMIGLTPAKAQELAKRYLEPGAAKDQVSKTAGD
                     LLRVQQRLRDAVRKEVTSQMKSMGVATQDDLDALRKRVRDLERRAGMTASGRKKTAAR
                     KKTAAKRTSGSGTSSTTAASSASS"
     gene            complement(6259..7029)
                     /locus_tag="VF036_05015"
     CDS             complement(6259..7029)
                     /locus_tag="VF036_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX6264623.1"
                     /translation="MLADPYDAILLDLDGVLYRWPEPIPGAADTIATLRDVGKRVAFV
                     TNNASRTPEEVAERLASVGVRAEPREVVTSALATAAALCERGVRSAFVVGEGGLVVAL
                     QDEGIRVVDGPAEDVGVVVVGFDRCVTYSKLKDASVLVARGVPLIASNADASFPAPNG
                     EAWPGAGALVAAIETTTGAAAELIGKPEAPLLELALDAAGGGRPLVVGDRLDTDVAGA
                     VRLGWDSVLVLTGSTRSEDVGRSEWKPTFVVDSVADLV"
     gene            complement(7033..7410)
                     /locus_tag="VF036_05020"
     CDS             complement(7033..7410)
                     /locus_tag="VF036_05020"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6264624.1"
                     /translation="MTVHADNEQRSAYDLYARGRSMLAGGHPHQAVMLLSQAKLLEPE
                     KASIREALGRALYACGRASRARREFAKAVQLDPSDDYAHFALALACERTGQRSRARGH
                     AKLAVVLRPDVPDYVRALERLSA"
     gene            complement(7512..7838)
                     /locus_tag="VF036_05025"
     CDS             complement(7512..7838)
                     /locus_tag="VF036_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX6264625.1"
                     /translation="MGADIVTVNVVVLAGTVAADPVQRRMPSGDEVTELRLSVPEAGR
                     RLLPLPVAAWHATVGAGPVDGIAKGDAVLVHGQLVRRFYRSGAGARSLTEVVATGIKK
                     LEQPAA"
     gene            7908..>8357
                     /locus_tag="VF036_05030"
     CDS             7908..>8357
                     /locus_tag="VF036_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6264626.1"
                     /translation="MERPQPVVFPVADGITAIDTFMGGRARYTAAYLLEARETALVET
                     GPGTSVEPVTDALHSLGVAPDELAHIVLTHIHLDHAGGVGRLAERFPSAAVWVHQRGA
                     PHLVDPARLVASTVRVWGEAEMRELFGPTEPVASGRVRSLEDADTIAL"
BASE COUNT         1229 a         3171 c         2806 g         1151 t
ORIGIN      
        1 ctctcggcca gatcggcgtc ggcgagcagc cacacggccg accccgggag acgacccagc
       61 gcctcctcga tcgcacgacc cttcccgagg cgttgggacg cccgcaggac cgtggcaccg
      121 gcgaggagcg cggccgagct cgtcgcgtcg gtggagccgt cgtccaccac gatcacctcg
      181 tcgaccagag cgtgcagcgc ggagacggtg ctggcgacgc gctgcgattc ctcgtgcgcc
      241 gccacgagcg ccacgacctg acgttccatc aggctcgaca ggatggcggc ggttcgtcgg
      301 caccgggaac gaccatcatc gcgtcgctcc cgaacccgta gtccccgccg ggccggaacg
      361 cgggatcgtc gtccacgagg aactgctcga tccccatgat catcgtgatg ccgccgatcc
      421 cctccagatc gaggtcatcg accgtgaccg acgcgcaatc ggcgatcccg ggcgcctctc
      481 ggatcgccga gacgagcccg aagaggtcgt cggtcgggcc gacgcccgac gtcggggtcc
      541 cgagctggac gaggcgttgg aagaacggga cgaggagcgt ctggggagtg atgcgatccg
      601 gcagggagcg accccccgcg gcgaccacca cgagttgtcc ggcaccgccg atgccaagga
      661 gggcgtcctc gtcgagatct cgatcggcgg tgatgagccc ggcggcgagg agcgcgccca
      721 gcaggtccgt gtccccgtcc ggtcccacgg ccagccggac ggccagcgcg tccgcgatcc
      781 gcgcctcgag ggagtcgggg tccgtcccgg tgacgccgag gatctcctcg atcgccgaca
      841 ccgtctcctc gtccggcaca tcggtgagcc gccgatcgat cgtgagcgtg gcgaggacgt
      901 cggcgcccgc cgcggtgagc tcgtcgacgg cgatctgggc cgggccgtcg acccccgcta
      961 ccgtgaccac caccgcttcc tcgccggtga gcaccccgtc tcggagggtc ggccccagag
     1021 catcggcgaa tcgctgctgg agggcaacca tgtcgagagc cgcgtcacgc tccccgatcg
     1081 cttcgtcgta gttcagctgc aggtcgcgag cgaaccgatc gccgacgacc gatgttcccg
     1141 ccaggatccc gagcccgaac gccagtatca ccgccacgat cgagacgacg tggtagcgcc
     1201 aggagatcac aggatcgcct gcagcaggtc cacgaagtcg tcgagcgtct gatccacgta
     1261 gagacggaga ccgcgtgacg cgaagagcat cagaccgaac gtgatgagca cggccccgac
     1321 caagatcacc acgtcccaga cccgcgcacg gctcgggtac aggcgggcca cgcctttcgc
     1381 gtccaccagg atctctccca cgcgcaaccg gacgaggaac gtcgaggaca tccctcggcg
     1441 gcccttgtcc aggaactccc gcaggttccc gtgcgtgccc acggcaacga tgagctccgc
     1501 ccgctgctcg tgcgcgagga ggaacgcgac gtcctcgctc gtgcccgccg agcggacttc
     1561 gacgtacggg atcccgagcc gctccaggcg agctcgcccc ggcgcacggc cgtcggggta
     1621 cgcatggagc acgatctccg tggtcgcccg tcgcgggcgc tggctcagat cgagcgctcg
     1681 atccgagatg ctgtccatgt ctccgatcac gacgtccggc acatggcccg cgtcgagcaa
     1741 cgcatcggcg ccaccatcca cgcccacgag gacggggtgg acgtcccgga tgtaggagcg
     1801 cagcgcggcc agatcctccc ggtagctggt gccccggacc acgacgagga catgtcgccc
     1861 ggcgagcgag tggctgagct ccgggaggcc ggcgccaccg aacagcaggt cgcgctcctc
     1921 gcgcatgtat tccaaggtgt tccgcgcgaa cccctcgaac cggtcgtcga gggcttcccg
     1981 agccgactcc atcgcctctc ggatcgacgc ccgatcctgt ctgatcccga ccccgacgag
     2041 tcgatcccca gtgaagacgc ggtcgtgttc gaggcgcacg ggatctcctt cgcggatctt
     2101 ctggagcacc agcgtgccga cccgatcgac gagtggcacg ccggcgtcct cgaggatcat
     2161 cggaccgagg ttggggtagc ggccgctgat cgagggcgcg gcattgacca cggcggccac
     2221 accgacccgg acgagtgctt ccgcagcgac ccggtcgagg tcctcgtgat cgatcacggc
     2281 cacgtccccg ggctcgatcc gcgtcacgag ctccttcgtg cgacgatcga tcctcgccct
     2341 tccccccgcc agggtgcggg ctgagcgtcg tcgaagcagc ttggaagcca ctggtccttc
     2401 cccggccagg attcgtcccc ggccgtccct cggatgggtt cgagcgtagc cgagaccgag
     2461 gctcaggtga cggcgcccgc cctgccgagg gacgcggaac cctcgcggag acgctccgcc
     2521 tcggcgagga gctcctccgc gtgggtcact gcgctctcac tgcccgcgag accggcgagc
     2581 atccgcgaga gctcctgggc ccggctcggt ccgtcgatca gctcgaccga cgccgtgccg
     2641 tcttccttgc tgacgcgcag gtgatggtcc gcgaagcacg cgatctgcgg caggtgggtc
     2701 accaccagca cctggcgacc cgcggcgaga cgcgccaagc gacgaccgac ggcgagtccg
     2761 gcctgcccgc cgatcccggc gtcgacctca tcgaaaacga gcgtgccgac ctcgtcgaga
     2821 tcggcgagca cgctccggca ggccagcagc acgcgggaga gctcgccgcc cgacgcggcc
     2881 ctggcgagcg ggcgagcggg ctgacccggg gatgccgaga accacagctc ggctcgttcc
     2941 gccccgccgg gacccggctc gtcgagcgga aggagccggg cctcgaagac ggcgcccggc
     3001 atcccgagct cctcgagctc tgcacggacc gcgtcggcga gggcgggcgc ggcgcgggtg
     3061 cgcccctccg tcacgatggc cgcacggtcc cgggcggcgg cggatgcggc ctccagctcc
     3121 cgctccagct ccgcgatccg atcgtcggcg ccggcgaggg tccgcagctg ctccgcggac
     3181 tcggccagga acgagacgac atcggcgtcg gtggggccgt acttgcgctg cagctgcttg
     3241 agcgccgcga cgcgatcccg aacggcctcc agccgctcgg ggtcgggcag gaggagttcc
     3301 ccgtatgagc ggacgtcccg ggcgagctcc gcgatctcgg cggcgatccc ccgcgcgcga
     3361 tccgccacct cgcgtgccga ggggtcgaga tccgcagcgt tctccaacag tgacgcgagg
     3421 gatgcggcgt cgtcggcgat cccctcctcc ccggtgaggg cgtgctccgc cgcggacgct
     3481 tgttccatca agcgctcgac atgcgccaac cgcccttcct cggcggtgag ctcctcggtc
     3541 tcgccgtccc gcgggccgac ggtctcgatc tcgtgcacct gataggcgag gagatcgagc
     3601 tcccgctcac ggtcccgcgc cgcttcgcgc agcgcgccga gctcggtagc gacgcctgta
     3661 cgtcgctcgt aggcctcgcg gtaggtgccg agcgcgacca catgcgcatc tccggcgtac
     3721 cggtctagga agccggtctg gacggccggc gccagcaggc gctgcgtctg accctgcccg
     3781 tggtgctcga cgagtccggc gccgagctcg gcgagcgccg acgccgtcgc gagctgacct
     3841 ccgatccggg ccgtcgatct gccgtcccgg gcgatcgacc gcgccaggat cacctcaccg
     3901 tcctccgccc agtcgccggc accgtcgatc gcgtcgaacc gcgcctggac ccgggcagcc
     3961 cgcgcatcct cccggacgag tgcggccgag ccccgctcgc ccaaggccag cagcagcccc
     4021 accgtcacca tcgtcttgcc ggcccccgtc tccccggtga ggacgttcag tccgggcgcg
     4081 agctcgagct cgagatcggc gatcaccccg aggccgctga tgtggagctc gcgcagcacg
     4141 gtcgggatgc taacggcctc ccccgacatc gggtgccctc gttccgggcg gatcgcccgg
     4201 gaggtcgaac ttgtcccgga cccgggtgag gaagcccgca gcgtcgtcct tcgacacgag
     4261 acgtgcgggg ctcggcgcag caaggatccg gatcctcgat cccaccggga gctcgatccc
     4321 ctcccgtcca tcggcggaca ggacaccggg ttcgtccccc accacctcca ggaccacgac
     4381 ctgtccggcg ccgagcacga acgatcgatc gaacaccatg tgcgcggcga tcggcgtcac
     4441 caccaggcag gggacgccgg ggctgacgat cggcccgcgg gccgagaacg agtacgcggt
     4501 cgatcccgtg ggggtcgcga cgatcacccc gtcggccgag aacgtggtga cgtactcgtc
     4561 gcctacgtgc accgcgagcc gaacgagacg gtggcgcgtc cacttctcga ccatgatctc
     4621 gttcatcgcc cactgggtcg cgaacgggtt cccgtccgcg ggctcggcca tcaccgcgag
     4681 ccgctcctcc acccgggcgc gcccgtcgag ggcggcgcgg atcagcacca tcgcgtcggg
     4741 ccccggctcg atctccgtga ggaagccgag ccggcccacc ttgacgccca ggaccgggca
     4801 gtccgcctct gccgcgaccc gcgcggctcg caggaacgtc ccgtcccctc ccacggaaac
     4861 caccaggtcc ggtcgctcct cgcgcacgtc gtccggcccg cgggcgatcc gcgtgtccac
     4921 ggactccgcc tgcaggtcgc ggcggagcga ctcagcggtc tcgaccgccg aagcgcgacc
     4981 ctcgtggacg acgaaggcga cgacgctcac gcgttcgtca gctcccgggc agcgccgatg
     5041 gcgacatcca cgccgaggtg ggcccgcggg ccgccgatcg tggcgcgcag caggaactcg
     5101 acgttgccgg caggacccgt gatcggtgac gggatcaccc cggctgcggg ggccccggct
     5161 tccgccgccg catcggccac cacgccgatc gcgcgacgcc agacgtcagg atccgtgacc
     5221 acgccaccgg ctcccacctc cgcccgcggc gcctcgaact gcggcttgac caggaggacg
     5281 aggtccgccc cccgatccgc cacgtcgacc agccgccgca ccacggtgcg gagcgaggtg
     5341 aacgcgaggt ctgccgtcac gagcgaggcc aggtacggga gcacttcccg gtcgagctct
     5401 cgaacgttcg tccgatcgag caccgtcacc cgcggatccg tccgaagccc ccaagcgagc
     5461 tggccgtagc cgacgtcgac ggcgacgacg tgtcgcgcgc cgcgctggag caagcaatcc
     5521 gtgaagcctc ccgtcgatgc gcccgcatcg agacagtcac gccccgaggg gtcgatcgcg
     5581 aacgcgtcga gggccgcggc cagcttcgcc ccaccgcggg agacgaacgg ccgcccgggg
     5641 cccgcgacga cgagcgcctc gtcggcactt acgagtgtcg tgctcttggt ggccggtgcg
     5701 cctcgcaccg tcacctgtcc ggtccggacc gcctcctgtg cctccgctcg gctgggtgcc
     5761 aggccccgac gtacgagctc gaggtcgagg gggcgccgac ccacgcgtcc gtcaggacga
     5821 ggccgacgac gcggccgtcg tcgacgacgt cccggagccc gacgtccgct tcgcggccgt
     5881 cttcttccgg gcggccgtct tcttccgacc ggacgcggtc ataccggcgc gccgctcaag
     5941 atcgcgcacc cgcttgcgga gtgcatcgag atcgtcctgc gtcgccacgc ccatcgactt
     6001 catctgcgag gtgacttcct tccggaccgc gtcccgaagg cgctgttgca cgcggagcag
     6061 gtcgccggct gtcttcgaga cctggtcctt cgcggctcca ggctcgaggt accgcttcgc
     6121 gagctcctgt gccttggcag gcgtgaggcc gatcatcgat tcgagcgctt tcctgacgtc
     6181 gtcgagcacc atgagatctg tccttccgtt cgctgtcgtg gacgcaggat acccccgcgc
     6241 cacctgggcg aggaccgctc acaccaggtc ggccacgctg tccacgacga acgtgggctt
     6301 ccattcggat cgccccacgt cctcgcttcg cgtcgaaccc gtgagcacga gcaccgagtc
     6361 ccagccgagt cgtaccgcgc ccgcgacgtc ggtatccagg cgatcgccca cgacgagcgg
     6421 gcgtccccca cccgccgcgt cgagcgcgag ctccagcaga ggtgcctccg gcttgccgat
     6481 cagttccgcc gctgcgccgg tggtcgtctc gatcgccgcg accagggcac cggcaccggg
     6541 ccacgcctca ccgttcgggg ccgggaagga cgcatcggcg ttcgacgcga tcagcgggac
     6601 cccgcgcgcc acgagcaccg acgcatcctt cagcttcgag tacgtgacgc agcgatcgaa
     6661 ccccacgacg acgaccccga cgtcctcggc cggtccgtcc acgacccgga tcccctcgtc
     6721 ctgcagcgcg acgaccagcc ccccctcacc gaccacgaac gccgaccgca cgcctcgctc
     6781 gcacagcgcc gcggcggtgg cgagtgccga cgtcaccacc tccctcggct cggcgcgcac
     6841 gccgaccgac gcgagccgct ccgcgacctc ttcgggcgtc cggctggcgt tgttcgtgac
     6901 gaaagccacc cgcttcccca cgtcgcggag ggtcgcgatc gtgtcggcgg ctcccgggat
     6961 cggttcaggc catcggtaca gaacgccgtc cagatcgagc aggatcgcgt cgtacggatc
     7021 ggcgagcacg cgtcaggcag acagccgctc gagcgcccgg acgtagtcgg gcacgtcggg
     7081 ccgcaggacg accgccagct tcgcgtgccc tcgggctcgc gagcgctggc ccgtacgctc
     7141 gcacgccagg gcgagtgcga agtgggcgta gtcatcggac gggtcgagct ggaccgcttt
     7201 cgcgaactcg cgccgagcgc gtgacgctcg gccacacgcg tagagggcgc gtccgagcgc
     7261 ctcgcggatc gacgccttct ccggctccag cagcttcgcc tgtgacaaca gcatcacggc
     7321 ctggtggggg tggccgcctg cgagcatcga ccgaccgcgc gcgtacaggt cgtacgccga
     7381 gcgttgctcg ttgtccgcgt gcaccgtcac ccgtccttcg gccccgaccg gacgcggcgg
     7441 gagtccccgt cgtgtccgct cgacgttagc agcgggaagg cgcaacgtgg ccgggctgga
     7501 cgatcgcgtc gtcaggcggc gggctgctcg agcttcttga tcccggtggc caccacctcc
     7561 gtcaggctcc gcgctcctgc gccactccga tagaaccgtc gaacgagctg cccgtgcacg
     7621 agcacggcgt ctcccttggc gatcccgtcg acggggcccg ctccgaccgt ggcatgccac
     7681 gccgcgaccg ggagagggag gaggcgtcgt cccgcctccg gaacggacaa tcggagctcc
     7741 gtgacctcgt ctcccgacgg catccgcctc tgcaccggat ccgcggcgac cgtacccgcg
     7801 agcacgacga cgttcaccgt cacgatgtct gcccccactt cgtgcctcct tccgggggaa
     7861 cggcgacggt atctcccgcc accgacacgg cctgcgacac tggtcggatg gagcgtcccc
     7921 agccggtcgt gttcccggtc gccgacggga tcaccgcgat cgacaccttc atggggggcc
     7981 gggcgcgtta cacggccgcc tacctcctcg aggccaggga gacggcgctg gtcgagacag
     8041 gaccggggac gtcggtcgaa ccggtcaccg acgcgctcca ctcgctcggc gtggcccccg
     8101 acgagctcgc ccacatcgtg ctcacgcaca tccacctcga tcacgcgggc ggcgtcggcc
     8161 ggctcgcgga gcgcttcccg agcgccgcgg tctgggtgca ccagcgcggc gcaccccatc
     8221 tcgtcgaccc ggcgcgactg gtggcgagca ccgtccgcgt ctggggggag gccgagatgc
     8281 gcgagctctt cggaccgacc gagcccgtcg cgagcggccg cgttcgctct ctggaggacg
     8341 cggacacgat cgcactc
//
LOCUS       DASUVE010000230         2970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_673455, whole genome shotgun sequence.
ACCESSION   DASUVE010000230 DASUVE010000000
VERSION     DASUVE010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673455"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1686)
                     /locus_tag="VF036_05035"
     CDS             complement(<1..1686)
                     /locus_tag="VF036_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264627.1"
                     /translation="MADLEPGMLATAWTVVQGSDPVSFDVEILGVLPDGIAPGVDFIL
                     VHTSGPVIDESGGIAAGFSGSPVYIGDDLVGAIAYGFSAADQTFGGVTPAEDMVRLLE
                     YPSGTGALGTTSAASRPAFAHEVRVPSQLRRTAASAADTAVEAIPTVAEPLRIPVTVS
                     GLNTRGFRRFAKTMRLGGLSLIPYRSGAASLGALAADPLAAGGAVAGAVSVGDLTVAG
                     VGTATAVCDDLLLAFGHPFFWDGRAGAHPMAMYGADVLQVIPDPSSLVGGFKIANLTD
                     VHGIIDQDRLTGIRGIEGMEPASVPVTSVVVNPDVVSIREGETTVFRQATDTFLWLPD
                     IAAFHLLGNQDTVFDRLGDGTVSLRFVLEGIGPDGESFRVVRPNMHFSEFDVSFESIV
                     ELFAFLFEIQDNPFGPVSFTSVHAESTITQEHLTAEIVRVESASSLQPGFRERARLRV
                     SPGDTISLRAFLLPEGATSEEMVELLVPVPRSRFGGSLTVGGGGADDCLFCFIEGEDG
                     EEDAPATFQALLRNLRRTPRNNDLVASLRVERGRERESISGTDTVILGEEQVEI"
     gene            2062..>2970
                     /locus_tag="VF036_05040"
     CDS             2062..>2970
                     /locus_tag="VF036_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX6264628.1"
                     /translation="MSRRVIPGLESPDPVLESEVRARLDRVEEALHKALAEADSQLLS
                     ATANYLLAAGGKRFRPMLAILSGYFGDPADPRLIPASVSLELVHVATLYHDDVIDEAE
                     TRHGVPAANVRWDNTVAILTGDYLFARASEVGADLGPEVCRILAQTIAVLCDGQIREV
                     DASRRLDQTEANYLEVIRRKTGALIAASCRLGAMMSDAPPEHVDILEAFGESLGMAFQ
                     LSDDIMDITSSQLELGKEPGVDMKEGVYTLPVLHALRTSPDREELARILSAGADGGLL
                     DRALAIVSDGDSIEHARAAVREEVARA"
BASE COUNT          489 a         1010 c         1020 g          451 t
ORIGIN      
        1 gatctcgacc tgctcttctc caaggatcac ggtgtcggtc cccgagatcg actctcgttc
       61 gcgtcctctc tcgacgcgga gggatgcgac gaggtcgttg ttccggggcg tgcgccggag
      121 gttccgcaac agcgcctgga acgtggccgg cgcatcttcc tccccgtcct ctccctcgat
      181 gaagcagaag aggcagtcgt cggcgccgcc gccgcccacg gtcagggagc cgccgaaacg
      241 gctgcgtggc accgggacga ggagctcgac catctcttcg gaggtcgcac cctcggggag
      301 taggaacgcc cgcaggctga tcgtgtcacc ggggctcacc cggagacggg cacgctctcg
      361 gaagccgggc tggagcgagc tcgccgactc gaccctgacg atctcggcgg tgaggtgttc
      421 ctgggtgatc gtggactccg cgtgcacgct ggtgaacgac acgggcccga acgggttgtc
      481 ctggatctcg aagaggaacg cgaataactc gacgatgctc tcgaaggaca catcgaactc
      541 cgagaagtgc atgttcggtc ggacgactcg gaacgactcc ccgtccggcc cgatgccctc
      601 gaggacgaat cggaggctca cggtcccgtc gccgaggcga tcgaacaccg tgtcttggtt
      661 cccgaggagg tggaacgccg cgatgtccgg gagccagagg aaggtgtcgg tcgcctgccg
      721 gaacaccgtc gtctcgccct cccggatcga cacgacatct gggttgacga cgacactcgt
      781 gacagggacg gaggcgggct ccatcccttc gatgccacgg atccccgtga ggcgatcctg
      841 atcgatgatg ccgtgcacgt cggtcaggtt ggcgatcttg aacccgccga cgaggctgct
      901 gggatccggg atcacctgaa gcacgtcggc tccgtacatg gccatgggat gggcgcctgc
      961 gcgcccgtcc cagaagaacg gatgtccgaa cgcgagcaag aggtcgtcgc acaccgcggt
     1021 cgccgttccc acccccgcga ccgtgagatc tccgacgctg accgcgccgg cgaccgcgcc
     1081 accggcggcg aggggatccg cggcgagcgc gccgaggctc gcggctccgg aacggtacgg
     1141 gatgagcgag aggccgccga gccgcatcgt cttcgcgaat cgacggaacc cgcgggtgtt
     1201 caatcccgag acggtcaccg ggatccgaag tggttccgcg accgtcggga tcgcctctac
     1261 ggcagtgtcg gcggcgctgg ccgcggttcg ccggagctga gacggaacgc gcacctcgtg
     1321 cgcgaatgcg ggtcgtgagg ccgcgctcgt cgtcccgagc gctccggtgc cggacgggta
     1381 ctcgagcagc cggaccatgt cctcggctgg cgtgaccccg ccgaacgtct gatccgccgc
     1441 ggagaaccca taggcgatcg ctccgacgag atcatctccg atgtagacgg gcgagcccga
     1501 gaagccggcg gcgataccgc cactctcgtc gatcaccggt ccggatgtgt ggacgaggat
     1561 gaagtccacg ccaggggcga tcccatcggg gagaacgccg aggatctcga catcgaagga
     1621 gacggggtca gagccttgga ccaccgtcca tgccgtcgcc agcatgccgg gctcgagatc
     1681 agccacgggg aagacatcgg gcgtggagct gcacaacgcg gccgcggaac ccgcgggagc
     1741 cgctatcgcg cctcccagca ggaccgcgga cgacaccagc agggtcacga cgcgcgaacg
     1801 agctcgtctg gttgctccca tcggatccac cccctcggct tgttctggcc cgacggtacg
     1861 ccacttgaga cgaccccgct cggcctgagg ttccttgcca cgtgagagat ctctgtgacg
     1921 gatctcgatc tgcccgatcg gacggaccgt tccgggtcat cgcggtaggc tgctcgtgaa
     1981 tccattcacg agtggccgag cagcgaggga ggcacccgta cccgaacggt ccgagatggg
     2041 cgcgcgctgg acacgacgaa cgtgagccgg cgtgtgatcc ccggtctgga gtcgcccgac
     2101 cccgtgctgg agtcggaggt ccgggcgcgc ctcgaccgcg tggaggaggc gctccacaag
     2161 gcgctcgcgg aggccgactc ccagctgctg tccgccaccg cgaactacct cctcgcggcc
     2221 ggagggaagc gatttcgccc gatgctcgcg atcttgtcgg ggtacttcgg cgatccggcg
     2281 gaccctcgac tgatccccgc ctccgtgtct ctcgagctcg tccacgtcgc cacgctgtac
     2341 cacgacgacg tgatcgacga ggcggagacc cgccacggcg ttcctgccgc gaacgtccgc
     2401 tgggacaaca ccgtcgcgat cctcacgggg gactacctgt tcgctcgcgc gtccgaggtc
     2461 ggcgcggacc tcggcccgga ggtctgccgg atcctggcac agacgatcgc cgtgctctgc
     2521 gacggacaga tccgtgaggt cgatgcctct cgccggctcg atcagaccga ggccaactac
     2581 ctcgaggtga tccggcgcaa gacgggcgcg ctgatcgccg cctcgtgccg gctgggggcc
     2641 atgatgtcgg acgcgcctcc cgagcacgtg gacatcctcg aggcgttcgg ggaatcgctc
     2701 ggcatggcct tccagctctc ggacgacatc atggacatca cctccagcca gctcgagctc
     2761 ggcaaggagc cgggcgtcga catgaaggag ggggtctaca ccctgcccgt gctccacgcc
     2821 ctccggacgt cacccgatcg ggaggagctg gcgcggatcc tgagcgcggg tgcggacggc
     2881 gggctcctcg accgcgcgct cgcgatcgtc tcggacggag actcgatcga gcatgcgcgc
     2941 gcggccgtgc gcgaagaggt cgctcgcgcg
//
LOCUS       DASUVE010000231         4165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1049868, whole genome shotgun sequence.
ACCESSION   DASUVE010000231 DASUVE010000000
VERSION     DASUVE010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049868"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VF036_05045"
     CDS             <1..542
                     /locus_tag="VF036_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264629.1"
                     /translation="DRALVQPLFDVERGNVEAFCRALHLKPRRDPMNEDRRYLRAAIR
                     HEVMPVLERETGRGVTRSIARTGDLLRADRDELFAAAVRGLEGVVDGVRGKEVRLDVT
                     ELLQLPRPVASRVIRLAGYNILSTDSASPWSKEAIDAVLDLAEGRPGRRRDLPEGLKA
                     VREKGYVRVTRSSPDPRES"
     gene            572..1150
                     /gene="hpt"
                     /locus_tag="VF036_05050"
     CDS             572..1150
                     /gene="hpt"
                     /locus_tag="VF036_05050"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012946836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX6264630.1"
                     /translation="MAAVSTLTDYVQAYQPDIERVLISSEEIQGKLAEMGEQISADYD
                     GSSLLLVGVLKGAFVVMADLARYVRLPLEFDFMAVSSYGAATKTSGVVRILKDLDHDL
                     EGMDVLLVEDIVDSGLTLKYLLKNLAARKPASLEVAALLRKTGIQKVPLDIRYVGFDI
                     PPDFVVGYGLDFAERYRNLPYIATLKPEAYGG"
     gene            1188..1559
                     /locus_tag="VF036_05055"
     CDS             1188..1559
                     /locus_tag="VF036_05055"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX6264631.1"
                     /translation="MKRIALVLVVVLAACGDGTGDGDGASGIRGRALAGPQCPVEMAE
                     SPCPDLPWEGTVVVTATDSGETFTASTDADGRFELSLSPGTYEVTIDAASTPPTTEPQ
                     TVTVEDGSYTEIEVFVDTGIR"
     gene            1676..3595
                     /gene="ftsH"
                     /locus_tag="VF036_05060"
     CDS             1676..3595
                     /gene="ftsH"
                     /locus_tag="VF036_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX6264632.1"
                     /translation="MAQPEPPFRRLARTPTLWILGAVVVFFGVLSLVQRPETAEEIDL
                     SEFWTFLEDGRIRNAEILEGDQVVRGELTDGTEYVATFTADLADDIANELREADVPIH
                     ATSQKPSGLVSLLYYVLPILLLAGLLLWMMNRSQGGSGRVMQFGRSRHKTVGKDQPKT
                     TFKDVAGVDEAIEELEEVKEYLQHPSKFQAMGAKIPRGVLLFGPPGTGKTLLARAVAG
                     EAGVPFFSISGSDFVEMFVGVGAARVRDLFEQAKASAPAIVFVDEIDAVGRHRGAGLG
                     GGHDEREQTLNQLLVEMDGFDQRSTVILMAATNRPDILDPALLRPGRFDRQIVVDRPD
                     LEGRKAILSIHAKGKPLDPGVDLDILARRTPGFTGADLANVINEAALLAARRSKKTIT
                     MQEVEEAVDRVMAGPERKSRLMSEQERRLIAYHEGGHAMVAHVLPNTDEVHKITVIPR
                     GRALGYTLTLPEQDRFMMTREELRDELAMLMGGRVAEEIIAGDISTGAGNDIERATKI
                     ARQMVTEHGMSDAIGPRTLGQKQGEVFLGRDWASTPDYSEAVAFEIDQEISRLIDEAH
                     DVALEILTQHRERLDELAALLLQKETLDRDEVEAFFKAVEKRAPRAPEVRSAGLAVSR
                     TEMFPPPDAPGPLRR"
     gene            3609..>4165
                     /locus_tag="VF036_05065"
     CDS             3609..>4165
                     /locus_tag="VF036_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6264633.1"
                     /translation="MTDRWATFDCYGTLIDWDGGVRRALVTVWPEEAGDRLFQRYHDV
                     EPRLQAGRGITYRQVLRETLVAIAEEDELEVPPGQDDALADSVPSWRPFPEVRGALAE
                     LRERGWKLAILSNTDPDMLGASLKTIGVEVDLRITAAEAGSYKPAHGHWDTFFRTTSA
                     DRARHVHVAASLYHDVEPCAQLGLP"
BASE COUNT          684 a         1400 c         1439 g          642 t
ORIGIN      
        1 gggaccgcgc gctcgtgcaa cctctgttcg acgtcgagcg cgggaacgta gaagccttct
       61 gccgtgctct tcacctcaag ccccggcgtg atccgatgaa cgaggatcgc cgctacttgc
      121 gagccgcgat ccgacacgag gtcatgccgg tcttggagcg cgagacgggc cgaggtgtca
      181 cccggtcgat cgcgaggacg ggagacctcc tccgtgccga ccgcgacgag ctcttcgccg
      241 ccgcggtcag gggactcgag ggtgtcgtcg atggcgtccg ggggaaggag gttcggctcg
      301 atgtcacgga actcctccag ctgccccgac ccgtcgcctc gcgggtgatc cggcttgccg
      361 ggtacaacat cctttccacc gactccgcat cgccgtggtc gaaagaggcg atcgacgccg
      421 tgctcgatct cgccgagggc aggcccggac ggagacgaga tcttcccgag ggcttgaagg
      481 ccgtccgcga gaaggggtac gttcgcgtca ctcgttcttc cccggatcct cgcgagagtt
      541 gatgggttct tcgacgcgcg aagggggagc gatggcagcc gtctctacac tgaccgacta
      601 cgtgcaggca tatcagcccg acatcgaacg cgtcctgatc tcctcggagg agatccaggg
      661 caagctcgcc gagatgggcg agcagatctc ggcggactac gacggctcct cgttgttgct
      721 cgtgggcgtc ctgaaaggcg cgttcgtcgt gatggcggac ctcgcgcgct acgtccggct
      781 cccgctggag ttcgatttca tggcggtgtc ctcctacgga gcggccacga agacctcggg
      841 ggtggtgcgg atcctgaagg acctcgatca cgatctcgag gggatggatg tgctgctcgt
      901 cgaggacatc gtcgactccg ggctcaccct caagtacctg ctgaagaacc tcgcggcgcg
      961 gaagcccgcg tcgctcgagg tcgccgcgct cctgcggaag accggcatcc agaaggtccc
     1021 gctcgacatc cgctacgtcg ggttcgacat cccgcccgac ttcgtggtcg gctacgggct
     1081 cgacttcgcc gagcggtacc gcaacctccc ctacatcgcc accctgaagc cggaggcgta
     1141 cggcgggtag cggaaccatc cggtcgctcg cgtccgtcga agccggtgtg aagcggatcg
     1201 ccctcgtcct cgtcgtcgtg ctcgccgcgt gcggcgacgg caccggcgac ggggacggcg
     1261 cctcggggat ccgtggtcga gcgctcgccg gtccccaatg ccccgtggag atggcggagt
     1321 cgccgtgtcc ggatctcccg tgggaaggca ccgtggtcgt gaccgcgacg gattccggcg
     1381 agacgttcac cgcctccacc gatgccgacg gccgcttcga gctttcgctc tcgccgggaa
     1441 cctacgaggt gacgatcgac gcggcatcga cgccgccgac cacggagccg cagaccgtca
     1501 cggtcgagga cggctcgtac accgagatcg aggtgttcgt cgacaccggc atccgctagt
     1561 ccgacctggc gcagcaggca cgggtgctac ccttgcggac gggctcgggc gcgcaacatg
     1621 ccggggcccg ttgcatcccc gacacggctc tctgtggccc tgaaagaggc gatccgtggc
     1681 ccagcccgaa cctccgttcc gacgcctcgc tcgcacgccg accttgtgga tcctcggcgc
     1741 ggtcgtcgtg ttcttcggcg tgttgtccct cgtccagcgc cccgagacgg cggaggagat
     1801 cgacctctca gagttctgga ccttcctcga ggacgggcgg atccgcaacg cggagatcct
     1861 cgaaggcgat caagtggtcc gcggcgagct cacggacggc acggaatacg tcgccacctt
     1921 caccgcggac ctcgccgacg acatcgcgaa cgagctccgg gaagccgacg ttcccatcca
     1981 cgccacgtcg cagaagccca gcggtctcgt gagcctcctg tactacgtcc tgccgatcct
     2041 gctcctcgcc gggctcctgc tctggatgat gaatcgttcc caaggcggca gcgggcgcgt
     2101 gatgcagttc ggtcgcagcc ggcacaagac cgtcgggaag gatcagccga agaccacgtt
     2161 caaggacgtc gcgggcgtgg acgaggcgat cgaggagctg gaggaggtca aggagtacct
     2221 gcagcacccg tcgaagttcc aggcgatggg agcgaagatc cctcgaggcg tcctcctgtt
     2281 cggccccccc ggcaccggca agaccctcct cgcgcgggcg gtggcaggag aggcgggtgt
     2341 gccgttcttc tcgatcagtg ggtcggactt cgtcgagatg ttcgtcggcg tcggcgccgc
     2401 tcgggtccgt gacctgttcg agcaggcgaa ggcgagcgct ccggcgatcg tgttcgtcga
     2461 tgagatcgac gccgtcggcc gccatcgcgg cgcggggctc ggcgggggcc acgacgaacg
     2521 tgagcaaacc ctgaaccagc tgctcgtcga gatggacggt ttcgaccaac ggtcgacggt
     2581 gatcctgatg gcagccacga accggcccga catcctcgat ccggccctgc ttcggccggg
     2641 gcgcttcgat cgacagatcg tcgtcgatcg gcccgacctc gagggccgga aagcgatcct
     2701 ctccatccac gcgaaaggca agccgctgga tccgggggtc gacctcgaca tcctcgcgcg
     2761 acgcactccc ggcttcacgg gcgccgacct cgcgaacgtg atcaacgagg ccgcgctcct
     2821 cgcggcacga cggtccaaga agacgatcac gatgcaggag gtcgaggaag cggtcgaccg
     2881 ggtgatggcg gggccggagc gcaagagccg cttgatgagc gagcaggagc ggcggctgat
     2941 cgcctatcac gagggtggtc atgcgatggt cgcgcacgtc ctcccgaaca ccgacgaggt
     3001 ccacaagatc acggtgatcc cgcgcggccg cgccctcggg tacacgctca cgctccccga
     3061 gcaggacagg ttcatgatga cgcgcgagga gctccgggac gagctcgcga tgctgatggg
     3121 cgggcgcgtc gcggaggaga tcatcgccgg cgacatctcg accggcgcgg gcaacgacat
     3181 cgagcgcgcc acgaagatcg cccgccagat ggtgacggag cacgggatga gtgacgcgat
     3241 cggcccgagg acgctcggac agaagcaggg cgaggtcttc ctcggccgcg actgggcctc
     3301 cacgcccgac tactcggagg ccgtcgcgtt cgagatcgac caggagatca gccggctgat
     3361 cgacgaggca cacgacgttg cgctcgagat cttgacccag caccgggagc ggctcgacga
     3421 gctcgcggca ctgctgctgc agaaggagac gctcgatcgc gacgaggtgg aggcgttctt
     3481 caaagccgtc gagaagcgcg cgccccgcgc cccggaggtc cgcagcgccg gcctcgcggt
     3541 gtcgcggacc gagatgttcc cccctcccga cgcgccgggc ccgctccgcc ggtgaaggga
     3601 cgtcggcgat gacggaccgc tgggcgacgt tcgactgcta cggcacgctg atcgattggg
     3661 acgggggcgt gcgaagggcg ctggtcacgg tctggccgga ggaggccggc gatcggctgt
     3721 tccagcgcta ccacgacgtg gagccgcgcc tccaggccgg gcggggcatc acctaccggc
     3781 aggttctgcg cgagacgctc gtcgcgatcg ccgaggagga tgagctcgaa gtccctccgg
     3841 ggcaggacga cgcgctcgcc gactccgtgc cgtcgtggcg accgttcccc gaggtgcgag
     3901 gggcactcgc cgagctccgc gaacgtggat ggaagctcgc gatcctgtcg aacaccgacc
     3961 cggacatgct cggcgcctcg ctgaagacga tcggggtgga ggtggatctg cggatcaccg
     4021 cggcagaggc cggctcgtac aaacccgccc acggacattg ggacacgttc ttccgaacga
     4081 cctcggccga ccgcgcccgc cacgtgcacg tcgcggcatc gctgtatcac gacgtcgagc
     4141 cgtgtgcaca gctcgggctc ccgtg
//
LOCUS       DASUVE010000232         3566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_861963, whole genome shotgun sequence.
ACCESSION   DASUVE010000232 DASUVE010000000
VERSION     DASUVE010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861963"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VF036_05070"
     CDS             <1..833
                     /locus_tag="VF036_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6264634.1"
                     /translation="TTFAQIVAGELGIQPEDIKVMHGDTDNTPYGLGTYASRSTPTSG
                     AATAMVARRVREKATKLAAHLLEVAEEDVEYEAGTFSVKGAPEQVKTIQEVAFAAYTN
                     YPEDMEAGLEGVHYYNPPNLTFPFGTYAVSVEVDPDTGVWEVLRVVAVDDCGVRINPM
                     IVEGQITGGLTEGYATAAMQLITFDEVGNAIGTNFMDYLIPTAWETPRFELGETVTPS
                     PHHPIGAKGVGESATVGSPAAYVNAVIDALQPLGVKNIDMPLTSAKVWEAIQAARTDA
                     "
     gene            855..1829
                     /locus_tag="VF036_05075"
     CDS             855..1829
                     /locus_tag="VF036_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX6264635.1"
                     /translation="MKPDILAEAAAMSARDEPFVLATVVWRRSPSSGKAGATALITSD
                     GRVRGWLGGACAEPTIVREAQRALEEGSPRLLFLGPPEELAGHERDGVVAVPIACQSE
                     GAMEVHVEPVLPQPQLVAVGRSPAAIALTAMAAALGWRSIAVDDDGDAADHPGAGRVL
                     TSLDLASANVDDRSFVVVATQGHYDEAALEQALATPAAYIGLVASRTRSEAVLGYLRD
                     RGANDDELARVHAPAGLDLGHLSSEEIAAAILAEIVQRKAAGDLRFARSEVPTAAHTA
                     IDPVCGMTVDVAVARYRSVHGGRTYHFCSAGCQASFEADPAAFVSVAR"
     gene            1852..2457
                     /locus_tag="VF036_05080"
     CDS             1852..2457
                     /locus_tag="VF036_05080"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6264636.1"
                     /translation="MLIEDRFTVRTPVDRLWAFIQDVERIAPCMPGAELTEVVDERTW
                     KGKVYVKFGPVQMAFAGTVVMEERDDAAYRAKLSARGTEQRGKGVANAKVESWLEPAG
                     DDGTTVQIRSDLTITGAAAQLSRGLLPEVSKLLTKQFADCLEAKLNEQLDVARTVTEA
                     EAEPVRAATPKAVGGFGLGLRALWAAIARFVARLFGRRSGS"
     gene            2454..3023
                     /locus_tag="VF036_05085"
     CDS             2454..3023
                     /locus_tag="VF036_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX6264637.1"
                     /translation="MISGIVLAAGSARRFGSTKQIATFRGRPLAQHALDALASAGIDD
                     IVVVTGHDADRVEAALALPPQGRFVRNADHASGQSSSLAVGLHDLAADSEGAVVLLAD
                     QPGVTDAEVRALTEAFGLSRSRIVRIVYTDGPGPALLSREVYAEAGHLHGDIGARAIM
                     ASHPDWVEEVVVPFPAPVDIDRPKDLPGD"
     gene            complement(3024..3446)
                     /locus_tag="VF036_05090"
     CDS             complement(3024..3446)
                     /locus_tag="VF036_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6264638.1"
                     /translation="MRRELSDGYELDDERTRVDVDVVHRYLAEEAYWVPGRDRATIER
                     LVRESARVIGAYHGREQVGFARVVSDGSSMAWLGDVFVLEGHRGHGLGVELVREAVEH
                     PAHRDLAWFLNTRDAHSLYERFGFEQPNERTMVRLRRS"
BASE COUNT          566 a         1223 c         1243 g          534 t
ORIGIN      
        1 agacgacgtt cgcccagatc gtcgccggcg agctggggat ccagcccgag gacatcaagg
       61 tgatgcacgg cgacactgac aacacgccgt acgggctcgg cacgtacgcg tcccggtcca
      121 cgcctacctc cggcgcggcc acggcgatgg ttgctcggcg cgtccgggag aaggcgacga
      181 agctcgcggc gcatctgctc gaagtcgccg aggaggacgt cgaatacgaa gccggcacgt
      241 tctccgtcaa gggagcgccc gaacaggtca agacgatcca ggaggtggcg ttcgccgcct
      301 acacgaacta cccggaggac atggaggccg ggctcgaagg cgtccactac tacaatccgc
      361 cgaacctcac gttcccgttc ggcacgtacg ccgtctcggt cgaggtcgac cccgataccg
      421 gcgtctggga ggtgctcagg gtggtggccg tcgacgactg cggcgtccgg atcaacccga
      481 tgatcgtgga gggacagatc accggcggcc tcaccgaggg atacgccacc gcggcgatgc
      541 agctgatcac tttcgacgag gtcggcaacg cgatcgggac gaacttcatg gactacctga
      601 tcccgaccgc gtgggagaca cccaggttcg agctgggaga gaccgtgacg ccttcgccgc
      661 atcacccgat aggcgcgaag ggcgtcgggg agtcggcgac cgtcggctcc cccgccgcct
      721 atgtgaacgc ggtgatcgac gcgctccagc ctctcggcgt gaagaacatc gacatgccgc
      781 tcacgtccgc gaaggtctgg gaggcgatcc aggcagctcg gacggatgcg tagtcggggc
      841 gaggacagga cggcgtgaag cccgacatcc tcgccgaggc cgccgcgatg tccgcccgag
      901 acgagccgtt cgtgctggcc acggtggtct ggcggcgttc gccctcttcc gggaaagcgg
      961 gcgccacggc gctgatcacg agcgacggcc gggtccgcgg gtggctcggt ggcgcgtgcg
     1021 cggagcccac gatcgtccgg gaggctcagc gcgcactcga ggaaggctcg cctcggctgc
     1081 tgttcctcgg cccgcccgag gagctcgcgg ggcacgagcg tgacggcgtc gtggcggtcc
     1141 cgatcgcatg ccagagtgag ggggcgatgg aggtgcacgt cgaaccggtc cttccccagc
     1201 cgcagttggt cgcggtcggt cgttcgcccg cggcgatcgc gctcaccgcg atggcggccg
     1261 cgctcggatg gcgaagcatc gccgtcgacg acgacggcga tgccgcggat catcccggag
     1321 cggggcgcgt cctcacgtcg ctcgaccttg cctctgcgaa cgtcgacgat cgatcgttcg
     1381 tcgtcgttgc cacgcagggc cactacgacg aggcagcgct cgagcaggcg ctcgcgacac
     1441 cggccgccta catcggtctc gtcgcctccc gcacccgctc cgaagccgtc ctcggctacc
     1501 tccgcgaccg cggcgcgaac gacgatgagc tcgcacgcgt gcacgccccc gccggcttgg
     1561 acctgggcca tctctcgtcg gaggagatcg ccgcggcgat cctcgcagag atcgtgcagc
     1621 gcaaggccgc gggcgaccta cggttcgcgc gttccgaggt gccgaccgcc gcgcacacgg
     1681 cgatcgatcc cgtctgcggg atgaccgtcg atgtcgccgt cgcgcgctac cgcagcgtgc
     1741 atggaggccg cacctatcac ttctgctcgg cagggtgcca agcatcgttc gaagccgacc
     1801 cggcggcgtt cgtctccgtc gcgcgatgag cgacggagcg cgagggaccg gatgctgatc
     1861 gaggaccgtt tcacggtacg cacaccggtc gaccgcctgt gggcgttcat ccaggacgtc
     1921 gagcggatcg cgccgtgcat gccgggcgcg gagctgacgg aagtcgtcga cgagcggacc
     1981 tggaaaggga aggtctacgt gaagttcggt cccgtccaga tggcgttcgc cggaaccgtc
     2041 gtcatggagg agcgagacga cgccgcctac cgggcgaagc tctccgcgag gggcaccgag
     2101 cagcgaggca aaggggtcgc gaatgcgaag gtggagtcgt ggctcgagcc tgccggcgat
     2161 gacggcacca cagtgcaaat ccgctcggac ctcacgatca cgggagcggc cgcccagctc
     2221 tcccgcggcc tcctcccgga ggtctcgaag ctgctgacca agcagttcgc ggactgtctc
     2281 gaggcgaagc tgaacgagca gctggacgtg gcgcggacgg tgacggaggc cgaggccgaa
     2341 ccggtgcgtg cggcgacgcc gaaggccgtc ggggggttcg gcctcggcct ccgcgccctg
     2401 tgggcggcga tcgcgcggtt cgtcgctcgg ctcttcggtc ggcgatccgg ctcgtgatct
     2461 cggggatcgt gctggcggcg gggtcggcgc gacggttcgg atccaccaag cagatcgcga
     2521 ccttccgagg acgaccgctc gcccaacacg ctctggatgc gctcgcgtcg gccgggatcg
     2581 acgatatcgt tgtcgtcaca ggccacgatg ccgatcgcgt cgaagcagcg cttgcgcttc
     2641 cccctcaggg ccgcttcgta cggaacgccg atcatgcctc cggacaatcg tcgtcgctcg
     2701 ccgtcggtct gcatgatctg gccgctgaca gcgagggggc ggtcgtcctg ctcgcggatc
     2761 aaccgggtgt caccgatgcc gaggtgcgtg cgttgacgga ggcgttcggg ctctcgcgga
     2821 gccgcatcgt gcggatcgtt tacacggacg gtcccgggcc ggcgttgctg tcccgcgagg
     2881 tctacgccga ggcaggccac ctgcacggcg acatcggcgc tcgcgcgatc atggcctcgc
     2941 atccggattg ggtagaagag gtcgtggttc ccttcccggc tccggtcgac atcgaccgtc
     3001 cgaaggacct gccaggcgac tgatcagctg cggcggaggc gcaccatcgt tcgctcgttg
     3061 ggctgttcga acccgaacct ctcatagagg ctgtgggcat cgcgggtgtt gaggaaccag
     3121 gcgagatcgc gatgcgccgg atgctccacg gcctcgcgga cgagttccac gccgagcccg
     3181 tgccctcgat ggccctcgag gacgaagacg tcaccgagcc acgccatgct cgagccgtcc
     3241 gagaccacgc gtgcgaaccc gacctgctcc cgaccgtgat acgcgccgat caccctcgcc
     3301 gactcccgta cgagccgctc gatcgtcgcc cggtcgcgac cgggcaccca ataggcctcc
     3361 tctgccagat accggtgcac cacgtccacg tcgacgcggg tgcgttcgtc gtcgagctcg
     3421 taaccgtccg agagctcgcg acgcatcagc agttgctgcc gagccgccga tgctcgagcg
     3481 cgggaagcac ccgacgcacg cggcggaggt ccgcgacgtc cagatcggcg aaccagacac
     3541 cggtcccctc ctccggtgcc tccacc
//
LOCUS       DASUVE010000233         3159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_298282, whole genome shotgun sequence.
ACCESSION   DASUVE010000233 DASUVE010000000
VERSION     DASUVE010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298282"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1914
                     /locus_tag="VF036_05095"
     CDS             <1..1914
                     /locus_tag="VF036_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX6264639.1"
                     /translation="QELTQDERDRRERMREQMTGVTTYDPDGGLTFATFVLGGRVHVA
                     DLVGGGAHPLEHAAEGAFDVRPDPTGRRVAYVVDGALHVQDLAGAGPGASSRILAHDD
                     DPDVQWGIAEFAASEELDRHRGFWWSPDGERIAAARVDERRVRVWHIASPVDPDAPPR
                     AVRYPQAGSDNADVSLSILGLDGSRVDVDWDRDAFPYLVEVTWNDRCPLTLLVLSRDQ
                     KRWLVLEADPDSGKTQALIEHTAEHWQTIVAGVPDRLSDGRLVFVEENEDTRRITLDG
                     EPVTPAGLQVASVLDVGDDILFAAQDDPTELHVWRTRGDGAGSPERLTTEPGVHTAAR
                     AGDLMVVVSDLADRRMRRAVAFRSGSELAEIRSHVESPVIDAHPTFGVLGDREIRVAV
                     FTPGGAEPPSPLPVIVDPYGGPHFAKVLNAYRAMLEPQWLADQGFAVLVADGRGTPGR
                     GVAWEHAVYRDFAGPVLDDQVDALQAAAERFGFLDLDRVGIRGWSFGGYLAAMAVLRR
                     PDVFHAAVAGAPVTDQTLYDTAYTERYLGHPQEEPDTYRRNSLIEDAPNLSRPLLLIH
                     GLADDNVFVANTLRMSRALTEAGRPHSVVPLSGITHMTTEESVAENLLLLQVRFLREA
                     LGLVDEVDAVHPA"
     gene            1950..>3159
                     /locus_tag="VF036_05100"
     CDS             1950..>3159
                     /locus_tag="VF036_05100"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018639026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX6264640.1"
                     /translation="MPIVVQKYGGTSVDGVERLRVVADRVVEARDAGNDVIVVVSAMG
                     QTTDDLARMAKDIAPEPDPREMDMLLTAGERIAMALLGIAINARGCRAASYTGSQAGI
                     ITDTAHGQARIIEIRPKRILEALDAGNVVIIAGFQGLSSAYEITTLGRGGSDLTAVAM
                     AAAVGAEVCEIYTDVGGVMTADPRIVPEARLIERIGYDDMLELAAAGAKVLQSRSVEL
                     ARRSGLRLHVRSSFDDEAPGTWVQEVDDDMEDVLISGVALETDEAKVTFDGIPDTPGV
                     AAALFKAVASAGVSVDMIVQNVSHEGVTDLSFTAPIADVSSLESTIGGIAQQVEARNW
                     SVDDGVAKISLVGAGMKSQPGVAADMFDALATEGINIEMISTSPIRISCVVRKADAER
                     ALRAVHARFGL"
BASE COUNT          472 a         1096 c         1133 g          458 t
ORIGIN      
        1 caggagctca cccaggacga gcgggaccgg cgcgagcgga tgcgcgagca gatgacgggg
       61 gtcacgacct acgaccccga cggtgggctc accttcgcga cgttcgtgct cggaggccgg
      121 gtgcacgtcg cggacctcgt cgggggcggg gcgcacccgt tggagcacgc cgccgaggga
      181 gcgttcgacg tcaggcccga ccccacggga cgccgcgtgg cctatgtggt ggacggcgcg
      241 ctccacgtgc aggacctcgc tggggcaggg cccggagcgt cgagccggat cctcgcccac
      301 gacgacgatc cggacgttca gtgggggatc gcggagttcg ccgcatcgga ggagctggat
      361 cgccaccggg ggttctggtg gtcgccggac ggtgagcgga tcgcggcggc gcgcgtggac
      421 gaacggaggg tgcgcgtgtg gcacatcgcg tcaccggtgg atccggatgc gccgccccga
      481 gccgttcgct acccgcaagc cgggtccgac aacgcggacg tgtcgctctc gatcctcggg
      541 ctcgacggtt cccgcgtgga cgtcgactgg gaccgcgacg ccttcccgta cctcgtcgag
      601 gtgacgtgga acgatcgatg cccgctcacc ctgctggtcc tgtcccgcga tcagaaacgc
      661 tggctcgtgc tcgaggccga ccccgactcc gggaagacgc aggcgctcat cgagcacacc
      721 gcggagcact ggcagacgat cgttgccggt gtgcccgacc gcctgtcgga cggacggctc
      781 gtgttcgtgg aggagaacga ggacacccgt cggatcacgc tggacggtga gccggtcacg
      841 ccggcgggtc tccaggtcgc gtcggtcctc gacgtcggcg acgacatcct cttcgcggcg
      901 caggacgatc ccaccgagct gcacgtctgg cggaccaggg gcgacggggc cgggagtccg
      961 gagcgtctga ccaccgagcc gggtgtccac accgccgccc gcgccggcga cctcatggtg
     1021 gtcgtctccg acctcgccga tcggcggatg cgtcgagccg tcgcgttccg ctcaggctcc
     1081 gagctcgcgg agatccgctc gcacgtcgag tcgccggtga tcgatgcgca ccccacgttc
     1141 ggcgtcctcg gcgaccggga gatccgcgtg gccgtgttca cgccgggagg agcggagcct
     1201 ccatccccgc tcccggtgat cgtggatccg tacggggggc cgcacttcgc gaaggtcctc
     1261 aacgcctacc gggcgatgct cgagccccag tggctcgcgg atcagggctt cgcggtcctc
     1321 gtggccgacg ggcggggaac gcccggccgc ggggtcgcgt gggagcacgc cgtctaccgc
     1381 gacttcgccg ggccggtgct ggatgaccag gtggacgcgc tccaggcggc ggcggagcgc
     1441 ttcgggttcc tggatctcga ccgcgtgggg atccgcggct ggtcgttcgg cggctacctc
     1501 gcggcgatgg ccgtgctccg tcgccccgac gtgttccacg ccgccgtcgc cggggcgccg
     1561 gtcacggacc agaccctcta cgacaccgcc tacaccgagc ggtacctggg acatcctcag
     1621 gaggagccgg atacctatag aaggaattcg ctgatcgagg acgcgccgaa cctctcgagg
     1681 ccgttgctcc tgatccacgg cctggccgac gacaacgtct tcgtcgccaa cacgttgcgc
     1741 atgtcccgcg cgctcaccga agccgggcga ccccattcgg tcgtcccgct ctccggcatc
     1801 acgcacatga ccaccgagga gtccgtcgcg gagaacctgc tcctgctcca agtgcggttc
     1861 ctgcgcgagg cgctcgggct cgtggacgag gtcgacgcgg tccatccggc ctgaccggtc
     1921 gccctcaaac gcactgctac gctcgcccca tgcccatcgt cgtgcagaaa tacggcggga
     1981 cctcggtcga cggcgtcgaa cggctccgcg tcgtcgcaga ccgggtggtg gaggcgcgcg
     2041 acgccggcaa cgacgtgatc gtggtcgttt ccgcgatggg acagaccacc gacgacctcg
     2101 cgcgcatggc caaggacatc gcgccggagc ccgaccctcg tgagatggac atgctcctca
     2161 cggcgggcga gcgcatcgcg atggcgctcc tgggcatcgc gatcaacgcc cgggggtgcc
     2221 gcgccgccag ctacaccggc tcgcaggccg ggatcatcac cgacacggcg cacggtcagg
     2281 ccaggatcat cgagatccgg cccaagcgga tcctcgaagc cctcgatgcc ggcaacgtcg
     2341 tgatcatcgc cgggttccag gggctctcgt ccgcctacga gatcaccacc ctgggacgag
     2401 gtggatccga cctcaccgcc gtggccatgg cggcggccgt gggagccgag gtctgcgaga
     2461 tctacacgga cgtgggcggc gtgatgaccg cggatccgcg gatcgtgccc gaggcccggc
     2521 tgatcgagcg gatcggctac gacgacatgc tcgagctcgc ggcggcgggg gcgaaggtgc
     2581 tccagtcgcg ctccgtggag ctcgcgcgga ggagcggcct ccggctgcac gtgcggtcct
     2641 ccttcgacga cgaggcaccc ggcacctggg tgcaggaggt cgacgacgac atggaagacg
     2701 tcctgatctc gggggtcgcc ctcgagaccg acgaagcgaa ggtgacgttc gacgggatcc
     2761 ccgacacgcc gggcgtcgcc gccgcgctgt tcaaggcggt cgcgagcgcc ggcgtgagcg
     2821 tcgacatgat cgtgcagaac gtctcgcacg aaggcgtcac ggacctgtcg ttcaccgcgc
     2881 cgatcgccga cgtctccagc ctcgaatcca cgatcggcgg catcgcgcaa caggtggagg
     2941 cacggaactg gtcggtcgac gacggcgtcg cgaagatctc gctcgtgggg gccgggatga
     3001 agagtcagcc gggcgtcgcc gccgacatgt tcgatgcgct cgcgacggag gggatcaaca
     3061 tcgagatgat ctccacctcc ccgatccgca tctcgtgcgt cgtgcggaag gcggacgcgg
     3121 agcgcgcact ccgcgccgtg cacgcgcgct ttggcctgg
//
LOCUS       DASUVE010000234         3967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1616368, whole genome shotgun sequence.
ACCESSION   DASUVE010000234 DASUVE010000000
VERSION     DASUVE010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616368"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VF036_05105"
     CDS             <1..410
                     /locus_tag="VF036_05105"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX6264641.1"
                     /translation="LEPGEMDEFVADEHSLAIEVDGVVVGAVQYGEEEDPMYRNATID
                     VYLTASRHGEGLGSEAVLVLARYLIEERGHHRLTIDPAAGNRAAIRAYEKVGFRRVGG
                     MRSYERGPAGTFHDGLLMEMLADELVTADETPS"
     gene            complement(444..956)
                     /locus_tag="VF036_05110"
     CDS             complement(444..956)
                     /locus_tag="VF036_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6264642.1"
                     /translation="MSYPEARYPGDRGEVSATYRPANQAPELTYPNGNTGHYLATGAS
                     TDGLFGLYRWVMGPEPSGPGPHFHRSLAESFYVLTGTVRIYDGTRWIDSEPGDFVHVP
                     IGGIHGFRNESGEPASLLIHFAPGAPREGYFEGLAEFAVSGMPDDDELAVFYLEHDNV
                     WLDERGRGHL"
     gene            complement(987..2372)
                     /locus_tag="VF036_05115"
     CDS             complement(987..2372)
                     /locus_tag="VF036_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018742381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6264643.1"
                     /translation="MLSRNDPELALRLSKDVDGRVIAPGDPDYDDARAVFYGGIDRRP
                     AVIVRVASDTDVARVIAEARETGLELAIRSGGHSLVGHSVTEGGIVLDLSEMQALEID
                     AESRTAWAESGVTAGAYSEAAAEHGLATGFGDAPSVGIGGITLGGGVGFLVRKYGLTI
                     DALLAADVVTAAGEVLRVDEESHPDLFWAIRGGGGNFGVATRFKFRLFDVPSIVGGIL
                     ILPATLETIVGWALASESAPEELSTIATVMKAPPMPFLPEAVHGKVVVLGMIVHVGGG
                     ETGERAVAPFRELAEPLADMLKEMPYPGIYMEEEGDFHPIVSDRTMFLDRVDEDVAAT
                     ILDRISSSDAMMAAAQFRVLGGAMARVRADATAFAHRSSKIMVNVAALFENVEDRPKY
                     EGWVQDFADGIRQDDPGAYVNFLEDEGPERVRAAYPGATWDRLREIKRRYDPTNLFRL
                     NQNVPPADEDA"
     gene            2499..3134
                     /locus_tag="VF036_05120"
     CDS             2499..3134
                     /locus_tag="VF036_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6264644.1"
                     /translation="MIDVAVIEDPEAAEVSLDPVRSRLLAELAEPGSATSLAGTLGLP
                     RQNVNYHLKALERHGLVELVEERKKGNMTERVVRATALSYVISPAALDGVQPDPSRAP
                     DRLSARWLIAVAARAVRDVGQLVAGATKAQKRLATFAMDGEIRFASAADRSAFAEELA
                     ASVTSLVSKYHDENAPRGRDHRLIVAVHPRLKGDVPTETTDAPAASDGEGH"
     gene            3136..3888
                     /locus_tag="VF036_05125"
     CDS             3136..3888
                     /locus_tag="VF036_05125"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6264645.1"
                     /translation="MAHPFELTHEIEVPATPEEVWDAIATGPGVDAWFMGVNEIEPRE
                     GGTTLMKFRGSSEEATVTTWQPPTHFASRTQEGEDGAVHAFEYLVEGRDKGSTVVRWV
                     HSGFLGDNWEKEYEGLSEGDPMYFDKLRVYLTYFRGRKAIPVNVFGGAVVPDREEAWA
                     TFHRGLGLAGPPQLGDRVGLTPEGLPQLDGEVDWLSRDFLGVRTDDGIYRFMHISVFG
                     GPTGVGHHIFVDGLDQRETEQAWESWLTKLFS"
BASE COUNT          645 a         1358 c         1344 g          620 t
ORIGIN      
        1 atctcgagcc aggtgagatg gacgagttcg tcgccgacga gcactcgctc gcgatcgagg
       61 tcgacggcgt ggtcgtgggc gccgtccagt acggcgagga agaggacccg atgtatcgga
      121 acgcgaccat cgacgtctac ctcacggcgt cgcggcacgg tgaaggactc gggtcggagg
      181 ccgtcctcgt gctcgcccgc tacctgatcg aggagcgcgg tcaccatcgg ctgacgatcg
      241 atcccgcggc agggaaccgc gcggccatcc gcgcctacga gaaggtcggg ttccgacgtg
      301 tcggcgggat gcgctcatac gaacgagggc ccgccggaac cttccacgat ggcctgctga
      361 tggagatgct cgcggacgag ctcgtcacgg cggacgagac cccctcctga cggaggcctg
      421 tggtgcggag gtcttccgcg ctcctacagg tgtccccgtc ccctctcgtc cagccagacg
      481 ttgtcgtgtt cgaggtagaa caccgcgagc tcgtcgtcgt cgggcatccc ggagaccgcg
      541 aactcggcga gtccttcgaa gtagccctcg cgaggcgcgc cgggcgcgaa gtggataagc
      601 aacgaagccg gctcgccgga ttcgttgcgg aacccatgga tccctccgat cgggacgtgc
      661 acgaagtcgc ccggctcgct gtcgatccag cgggtcccgt cgtagatccg aaccgtgccg
      721 gtcagcacgt agaaggactc cgcgagcgac ctgtggaagt gcggccccgg accactcggc
      781 tctggaccca tgacccatcg gtacagcccg aacagaccgt cggtggacgc gcccgtcgcg
      841 aggtagtgac ccgtgttgcc gttggggtag gtgagctccg gagcctggtt cgctggacga
      901 tacgtcgcgc tcacctctcc gcgatcaccg gggtacctcg cttccgggta actcatcgag
      961 ccggctctcc tctcatccac ggagcctcat gcgtcctcgt ccgccggtgg gacgttctgg
     1021 ttcaggcgga acaggttcgt cgggtcgtat cgccgcttga tctcacgcag ccgatcccag
     1081 gtcgcgcccg ggtaggcggc gcggacgcgc tcggggccct cgtcctcgag gaagttcacg
     1141 tacgcgcccg ggtcgtcctg ccggatgcca tcggcgaagt cctggaccca tccctcgtac
     1201 ttcgggcggt cttcgacgtt ctcgaagaga gcggcgacgt tcaccatgat cttgctcgag
     1261 cggtgcgcga aagccgtcgc atccgcgcgc acacgcgcca tcgcgccgcc gagcacgcgg
     1321 aactgggccg cggccatcat cgcgtccgac gagctgatcc ggtcgaggat cgtggcggcg
     1381 acgtcctcgt ccacgcgatc gaggaacatc gtccggtccg acacgatcgg gtggaagtcc
     1441 ccttcctcct ccatgtagat cccggggtac ggcatctcct tcagcatgtc ggcgagtggc
     1501 tccgcgagtt cgcggaaggg cgccaccgcc cgctcgccgg tctctccgcc gccgacgtgc
     1561 acgatcatcc ccaagaccac gaccttgccg tggaccgcct cggggaggaa cggcatcggc
     1621 ggcgccttca tcacggtcgc gatcgtcgag agctcctcgg gagccgactc cgaggcgaga
     1681 gcccagccca cgatggtctc gagagtggcg ggcaggatca ggatccctcc cacgatcgac
     1741 ggcacatcga acaagcggaa cttgaaccgg gtcgccacac cgaagttgcc gccgccgccg
     1801 cggatcgccc agaacaggtc ggggtgcgac tcctcgtcca cccgcaggac ctcgccggcc
     1861 gcggtcacga cgtcggccgc caggagtgcg tcgatcgtga ggccgtactt ccgtaccagg
     1921 aagccgaccc ctccgcccaa cgtgatcccg ccgatgccca ccgaaggggc gtcaccgaac
     1981 ccggtcgcga ggccgtgctc ggcggccgcc tccgagtagg cgccggccgt gacaccggac
     2041 tccgcccacg cggtacggct ctcggcgtcg atctcgagcg cctgcatctc cgagaggtcg
     2101 aggacgatcc caccctcggt gacggagtgc ccgacgaggc tgtggccgcc gctccggatc
     2161 gccagttcga ggcccgtctc acgagcctcg gcgatcacgc gggcgacgtc agtgtcgctc
     2221 gcgacgcgga cgatcaccgc cggccgacga tcgatcccgc cgtagaacac cgcccgggca
     2281 tcgtcgtagt cgggatcgcc gggcgcgatc acgcgcccgt cgacgtcctt gctgagccgg
     2341 agcgcgagtt cggggtcgtt cctcgacagc atggatcctc ctcgtgttcg gggtcgccct
     2401 tcctgtctac aacccgcctc ggtgaacggc cacccggatg tccaccgaag atttctctga
     2461 tcttgacaaa aagaactgtc ggtggcaaca tggcactcgt gatcgatgtg gccgtgatcg
     2521 aagaccccga ggcagccgag gtttccctgg accccgtgcg gagccgcctg ctggccgagc
     2581 tggccgaacc cggctccgca acgagcttgg ccggcacgct cggtctccct cgccagaacg
     2641 tgaactacca cctcaaggcg ctcgagcggc acggcctcgt ggagctcgtc gaggaacgga
     2701 agaagggcaa tatgacggaa cgggtggtcc gtgcgacggc gctctcctac gtgatctctc
     2761 cggctgccct cgacggcgtc cagccggacc cgtcccgcgc cccggaccgg ctctccgcgc
     2821 ggtggttgat cgcggtggcg gcgagggccg tgcgggacgt gggacagctc gtggccggcg
     2881 cgacgaaggc tcagaagcgc ttggccacgt tcgcgatgga cggcgagatc cggttcgcct
     2941 cggcggccga ccggtcggcc ttcgccgagg aactggccgc ttcggtcact tcgctggtaa
     3001 gcaagtacca cgacgagaac gctccgcgcg gtcgcgacca ccgcctgatc gtggcggtcc
     3061 acccgagact caagggcgac gttccgaccg agacgacgga cgcgccggcg gcatccgacg
     3121 gagaggggca ctgagatggc acacccgttc gagctgaccc acgagatcga ggtcccggcg
     3181 acccccgagg aggtgtggga cgcgatcgcc accggccccg gcgtcgacgc ctggttcatg
     3241 ggtgtgaacg agatcgagcc tcgcgagggc gggacgaccc tcatgaagtt ccgcggctcc
     3301 tcggaggagg ccacggtgac gacatggcag ccgcccacac acttcgcgag ccgcacgcag
     3361 gaaggtgagg acggcgcggt ccatgcgttc gagtacctcg tcgaaggtcg ggacaagggc
     3421 agcaccgtcg tccggtgggt ccacagcggc ttcctcgggg acaactggga gaaggagtac
     3481 gagggcctct ccgagggcga cccgatgtac ttcgacaagt tgcgcgtcta cctcacgtac
     3541 ttccgcggac ggaaggcgat cccggtgaac gtgttcggcg gagcggtggt ccctgaccgc
     3601 gaagaggcgt gggcgacgtt ccatcgcggg ctcgggctcg cgggtccccc gcagctcggc
     3661 gatcgggtcg gactcacgcc cgaggggctc ccacagctcg acggcgaggt ggactggctc
     3721 tcccgggact tcctcggtgt ccgcaccgac gacgggatct accggttcat gcacatcagt
     3781 gtgttcggcg gtccgacggg cgtcggccat cacatcttcg tcgacggcct ggatcagcga
     3841 gagaccgagc aggcctggga gtcctggctc acgaagctgt tctcgtaagc tcgaaccgag
     3901 gaggggatat ccccatggaa acggtcacct cgagggacgg gaccgccatc gccttcgatc
     3961 ggatggg
//
LOCUS       DASUVE010000235         2055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1428767, whole genome shotgun sequence.
ACCESSION   DASUVE010000235 DASUVE010000000
VERSION     DASUVE010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428767"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VF036_05130"
     CDS             <1..427
                     /locus_tag="VF036_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264646.1"
                     /translation="RTMLPNPVEQLLAPLLPFVGGIIGAVEPWGGRIGFVIQLFLIPA
                     IVYAIWRRGDVLRRFALMVVPAWAHWIVLGLAIVRTRFHVVSWAMFVVGLRIAAEDAS
                     ARYPRAKLWIEIAWTACILLGLIDVSVEMVRHLQSWGAA"
     gene            complement(424..1458)
                     /locus_tag="VF036_05135"
     CDS             complement(424..1458)
                     /locus_tag="VF036_05135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6264647.1"
                     /translation="MGTQGTPRIAVIIPCHDEQAAIATVVHGVRAAVPDAEIYVYDNA
                     STDRTADVAAHAGANVRREPVKGKGNVIRRAFADVDADIYVLIDGDDTYATQDLPAMI
                     ALLQSGPYDHVLGVRSGDATAYRPGHALGNRAFNWLTGTVFGASVSDMLSGYRVLSRR
                     FVKSFPTLSRGFEIETELTVHAMGLRAPQVEVPVGFKDRADGTQSKLHTVRDGVRILS
                     LILTLIRHERPILYFGLIGAIFAVIGLVLGIPVVVEYFETGLVRRFPTAGLALGMILI
                     AFMSWMIGVILDGVLKARRELARLSYLRFSPPPFQPDGPTTVEAEQAGQAERVERAER
                     VERAERAEQP"
     gene            1565..2017
                     /locus_tag="VF036_05140"
     CDS             1565..2017
                     /locus_tag="VF036_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX6264648.1"
                     /translation="MSDESPRQPGVLRRGGAFLVIGALAFVLDAAVFNLLTFGITGEG
                     PMYDSPVWSKSIAIAVATVFTYVGNRYWTYRSRRIERRLSRYVLFVAVNAAAIGIQLG
                     CLAFSRYVLGLEGIVPDNIAGTFVGQALATGFRFFAYDRWVFRERQPV"
BASE COUNT          280 a          688 c          723 g          364 t
ORIGIN      
        1 tcgcaccatg ctgccgaacc cggtcgagca gctgcttgct cctctcctgc cgttcgtcgg
       61 cggcatcatc ggcgccgtcg agccgtgggg tggccggatc gggttcgtga tccagctgtt
      121 cctgatcccg gcgatcgtgt acgcgatctg gcgccgcggt gacgttctgc gtcggttcgc
      181 gttgatggtg gttccggcgt gggcgcactg gatcgtgctc ggcctggcca tcgtgcggac
      241 ccggttccac gtggtctcgt gggcgatgtt cgtcgtggga ctgcgcatcg ccgcggagga
      301 cgcgtcggcg cgctaccccc gagcgaagct ctggatcgag atcgcctgga ccgcgtgcat
      361 cctcctcggc ctgatcgatg tctcggtcga gatggtccgg catctccagt cgtggggtgc
      421 cgcctagggc tgttcggccc gttcggcccg ttcgacccgt tcggcccgtt cgacccgttc
      481 ggcctgtccg gcctgctcgg cctccacggt ggtcggcccg tcgggctgga acggcggcgg
      541 agagaaccgg aggtagctga gccgcgcgag ctcgcgccgg gccttgagca cgccgtcgag
      601 gatgacgccg atcatccacg acatgaacgc gatgaggatc atcccgagcg cgagccccgc
      661 ggtcgggaag cggcgcacga ggccggtctc gaagtactcg acgaccacgg ggattccgag
      721 cacgaggccg atgaccgcga agatcgctcc gatgagcccg aagtagagga tcggccgctc
      781 gtggcggatg agcgtgagga tcagggagag gatccggacg ccgtcgcgga ccgtgtgcag
      841 cttgctctgc gtgccgtcgg cccggtcctt gaagccgacc ggcacctcga cctgcggagc
      901 tcggaggccc atcgcgtgca cggtcagttc ggtctcgatc tcgaaccccc gggagagggt
      961 cgggaagctc ttcacgaagc gccgggacag cacccggtag ccgctcagca tgtcgctgac
     1021 cgaggcgccg aacacggtgc ccgtcaacca gttgaaggca cggttcccga gcgcgtggcc
     1081 aggccggtac gcggtggcgt cgcccgaccg cactccgagc acgtggtcgt agggaccgga
     1141 ctggagcagc gcgatcatcg ccgggagatc ctgcgtcgcg taggtgtcgt caccgtcgat
     1201 gagcacgtag atgtccgcat cgacatcggc gaaggcgcgc cggatcacgt tgcccttgcc
     1261 cttcaccggc tcgcgacgca cgttcgcccc ggcatgcgcg gcgacgtcgg ccgttcggtc
     1321 ggtgctggcg ttgtcgtaca cgtagatctc ggcgtcgggg accgcggcgc ggaccccgtg
     1381 cacgacggtg gcgatggccg cctgctcgtc gtggcacggg atgatgactg cgatccgggg
     1441 tgttccctgc gtgccgatgt ccgcctccga ttcccgggtg agaccgcgat cgggccgcgc
     1501 tcacggtccc attatcattg gacagatcat cgcgcgagca ccaccacccg gaagggggca
     1561 gcccgtgtcg gacgagagcc cccggcaacc cggagtgctc cggcgcggcg gcgcgttcct
     1621 cgtcatcggg gcgctcgcgt tcgtgttgga cgcggccgtc ttcaaccttc tcacgttcgg
     1681 aatcacgggc gaggggccca tgtacgactc cccggtctgg tcgaagtcga tcgcgatcgc
     1741 ggtcgcgacg gtcttcacct acgtcgggaa ccggtactgg acctaccggt cgcgccggat
     1801 cgagcgtcgt ctctcgcggt acgtcctctt cgtggcggtc aacgccgcgg cgatcgggat
     1861 ccaactcggg tgcctcgcgt tctcgcgata cgtcctcggg ctggagggca tcgtgcccga
     1921 caacatcgcc ggaacgttcg tcggccaggc actggcgact ggcttccggt tcttcgccta
     1981 cgaccgctgg gtcttcaggg aacgtcagcc ggtgtgagct cggtcgacgc ccgcgatgac
     2041 ctccccttcg cggtg
//
LOCUS       DASUVE010000236         5263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_676017, whole genome shotgun sequence.
ACCESSION   DASUVE010000236 DASUVE010000000
VERSION     DASUVE010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676017"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VF036_05145"
     CDS             complement(<1..208)
                     /locus_tag="VF036_05145"
                     /inference="COORDINATES: protein motif:HMM:NF045421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264649.1"
                     /translation="MCEVGSTKLGYQLRCIEDLHAMLKEQGDWIELGSADEQRTAKDG
                     TVEAWGRSADNPVGGWYGIKKSLRG"
     gene            418..963
                     /locus_tag="VF036_05150"
     CDS             418..963
                     /locus_tag="VF036_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264650.1"
                     /translation="MRRVPDAFLRNMAEVTATLIGLFLVGVFFYVETGFRRWDRSCDV
                     FEKYLRSGTRITLIVLSIALGMSLSLVVMDPAWTRILFVVLSLVLIAANVDSALHVRG
                     VRRATGSIALVINEIVTTAMTLALLALPWILGGLSPTRRDFAWSILLAFAAGLLSIGA
                     IVMSAFDVARPTEAGEGESPS"
     gene            996..1706
                     /locus_tag="VF036_05155"
     CDS             996..1706
                     /locus_tag="VF036_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017681577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6264651.1"
                     /translation="MTNPSIILVPGFWLGAWAWDEVAGLLRTEGQEVTALTLPGLESA
                     HVDRSSIGLSHHVDAITEAVKAAGDPVVLAVHSGTGFSGYVASDRVPGSITAMVYVDT
                     APGIPPLDADFDGDEKPLAWEEVSAEENLDGLSEEQLETFRQRAVPEPGGVLRDTLEL
                     TNEARLDLPSTIICTGYTSQQYKEAMGEGYAWLAGLQEVRNITWVDLPTSHWPMWSRP
                     KELAEIIGDVAKAHASGG"
     gene            1735..2508
                     /locus_tag="VF036_05160"
     CDS             1735..2508
                     /locus_tag="VF036_05160"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6264652.1"
                     /translation="MGFEDLMRDRSAAQYADFVLHAIGRDDRVLDVGCGPGSITLGLA
                     QTAGHVTGIDVDDAEFADGRAYAAGHGIDNVDFREGSIYDLGVAEGSIDVCTLFAMLE
                     TLDDPLAGLAEVLRVLKPGGVIGVSSIEYGGLILHGADVPPLRRFYELRLAIWKAQGD
                     VHPYRGRGLRGLLLAAGFAQVEASATYFSYGTEERVRTFGLGRAADCRDEWFVDGLTE
                     RGLADRNEIDALEQAWIRWADGPDSFAAFAWGRATARKA"
     gene            2577..3284
                     /gene="folE"
                     /locus_tag="VF036_05165"
     CDS             2577..3284
                     /gene="folE"
                     /locus_tag="VF036_05165"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEX6264653.1"
                     /translation="MRTDPETPDGQRSTARPADVGASWAMSDPEETTSWERVRPREVT
                     DEQWDRFEDAVAEMFAAFGMDLQTEGTHNTPRRFVRAMYEATTGYEGDPKLLTAFPTE
                     CRGGPDCSISQVIEGPIPFFALCEHHAFPFYGVAHVGYVAHEQIIGISKMTRLVRVYA
                     RRFTVQERLGQQIADHLNEVMDPHGVAVHLQAAHLCTQMRGVREERSRTSTSFWRGNY
                     ERDPEMRREFLTVAQRE"
     gene            3281..4090
                     /locus_tag="VF036_05170"
     CDS             3281..4090
                     /locus_tag="VF036_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6264654.1"
                     /translation="MTALDVHLEAVFDAGGAPDIPVPEEIVSAYGGSFRLPERVVYAN
                     FVSSIDGVAAIGGVEMSSAEISGGGAADRFVMALLRAVADAVVVGAGTVREHDGPWTA
                     EKAFPPGAEILSRLRAGISASESPTLVVVTASGELPAEHPALADAIVVTTSAGTRSLA
                     EGGARVGEVIDVGDADRVDGAVAIDRLRERGYQRILTEGGPRLMGAMLESSAVDQLFL
                     TISPKLIGGGPERPPLTDETDLLEREVGARLLSIRRSDDYLFLRYGLSSPD"
     gene            complement(4121..4597)
                     /locus_tag="VF036_05175"
     CDS             complement(4121..4597)
                     /locus_tag="VF036_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264655.1"
                     /translation="MSVLGALAGGLLGTFVLTTVLRTASELGLTRVDLPFLLGTAVTK
                     DRARAKAIGYVAHFGFGLLFSLAYAVFFLVVDASGWLLGALLGLAHGLFAGTALVNVL
                     LPVVHPRMGRPWTDARSSPLIESPGFLMLNYGAGTPVVSLIAHILYGAIVGGLAAW"
     gene            complement(4594..>5263)
                     /locus_tag="VF036_05180"
     CDS             complement(4594..>5263)
                     /locus_tag="VF036_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX6264656.1"
                     /translation="RLARIADLVCEIWRTPDSGIWEVRSEPRHYTQSKMMCWVALDRA
                     IALAASTRVPSDRVDRWVRERDAIEAFVEERCWSDRLHSYVRSDGSEDLDASVLHASI
                     LGYGEPAGERMLGTMAAIDRHLRNGPLVMRYVVDDGLPGEEGAFLACSFWLADAWARA
                     GRVDEAEQLMERLIAMANDVGLYAEEIDPADGSFLGNFPQALPHLALIGAATAIEEAR
                     RGRT"
BASE COUNT          800 a         1827 c         1827 g          809 t
ORIGIN      
        1 ggccccgcag actcttcttg atcccgtacc agccgccgac gggattgtcc gccgaccgcc
       61 cccacgcctc gacggtgccg tccttcgcgg tcctctgctc gtcggcgctc cccagctcga
      121 tccagtcgcc ctgctccttc agcatcgcgt ggaggtcctc gatgcaccgg agctggtagc
      181 cgagcttcgt cgaaccgacc tcgcacacca gggtcggcgg tcggcctcct cgtcgcgcca
      241 catccggtac tcggacgtgc ccggcggggt cttcaagccc atggatcctc gcgcgttccg
      301 cttcccgaca tcacgtgctc ctttcctgag cgccggacgt gcgttcgacc gcttcggagg
      361 ccacgtggcg agcctcccag accgaacgat gaccgatgga ggataggctt cggggccgtg
      421 cggcgggtgc cggacgcctt cctgcgcaac atggcggagg tcacggccac gctgatcggt
      481 ctgttcctcg tcggggtgtt cttctacgtc gagaccggct tccggcgatg ggaccgctcg
      541 tgcgacgtct tcgagaagta cctgcgctcg ggcacgcgca tcaccctgat cgtcctgtcg
      601 atcgcactcg gcatgtcgct gtcgctggtt gtgatggatc cggcatggac gcgcatcctc
      661 ttcgtggtgc tgagcctggt actgatcgcg gcgaacgtgg actccgccct ccacgtccga
      721 ggcgtcaggc gagccacggg gtccatcgcg ctggtgatca acgaaatcgt caccacggcg
      781 atgaccctgg cgttgctcgc cctgccgtgg atcctgggtg ggctttcccc cacgagacgg
      841 gacttcgcat ggtcgatcct cctcgcgttc gcggccgggc tgctcagcat cggcgcgatc
      901 gtgatgtccg cgttcgacgt ggcccgaccc accgaagcgg gcgagggcga gtctccaagc
      961 tgacggatgt cccgacccac gcctagggtc gggggatgac gaatccatcg atcatcctcg
     1021 ttcctgggtt ctggctcggt gcgtgggcgt gggacgaggt cgccggcctg ttgcggaccg
     1081 agggccaaga ggttaccgcg ctcacgttgc ccggcctcga gtccgcccat gtggatcgct
     1141 cctcgatcgg cctgtcccac cacgtcgacg cgatcaccga ggcggtcaag gcggcgggcg
     1201 atcccgtcgt gctcgccgtg cacagcggga ccggtttctc gggctacgtt gcgagcgatc
     1261 gggtccccgg gtcgatcacg gcgatggtct acgtcgacac cgccccgggc atcccgcccc
     1321 tcgatgccga cttcgacggc gatgagaagc cgctggcgtg ggaggaggtc tccgcggagg
     1381 agaacctcga cgggctcagc gaggagcagc tcgagacgtt ccgccagcgg gcggtcccgg
     1441 agccgggcgg cgtcctccgc gacaccctcg agctcaccaa cgaggcgcgg ctcgacctcc
     1501 cgagcacgat catatgcacg ggctacacgt cccagcagta caaggaggcg atgggcgagg
     1561 gctacgcctg gctcgccggc ctccaggagg tgcggaacat cacctgggtc gacctgccga
     1621 cgagccactg gccgatgtgg tcacgtccga aggagctcgc cgagatcatc ggcgacgtcg
     1681 cgaaggcaca cgcctccggc ggctgaccca cgcgactgac gggagcgcac ggtcgtgggg
     1741 ttcgaggacc tgatgcgaga tcgaagcgcc gcgcagtacg cggacttcgt gctccacgcg
     1801 atcggtcgcg atgatcgggt cctcgacgtg ggctgcgggc cgggttcgat caccctgggc
     1861 ctcgcgcaga ccgccgggca cgtgaccggg atcgacgtcg acgatgcaga gttcgcggat
     1921 gggcgcgcgt acgccgccgg ccacggcata gacaacgtcg acttccgcga agggagcatc
     1981 tacgacctgg gcgtcgcgga ggggtcgatc gatgtctgca cgctcttcgc gatgctggag
     2041 acgctcgatg acccgctcgc gggcctcgcc gaggtcctcc gcgtactgaa gcccggtggc
     2101 gtcatcggtg tgtcgtcgat cgagtacgga gggttgatcc tccacggcgc ggacgtgcct
     2161 ccgttgcgcc ggttctacga acttcgactc gcgatatgga aagcgcaggg ggacgtccac
     2221 ccctaccgcg gccgagggct ccgcggtctg ctccttgcgg cgggattcgc gcaggtcgag
     2281 gcatccgcca cgtacttcag ctacgggacc gaggaacgcg ttcggacgtt cgggctcggt
     2341 cgcgccgccg attgccggga cgagtggttc gtggacggac tgaccgaacg aggactcgcc
     2401 gaccggaacg agatcgatgc gttggagcag gcctggatcc ggtgggcaga tggacccgac
     2461 tcgttcgccg cgttcgcctg gggcagagcc acagcacgga aggcctgagc caaacgcccg
     2521 gcaggaccgt acggtgggtg gaggaagcaa ccgatgaagc gaggcggagg ccgaggatga
     2581 gaaccgatcc agagaccccg gacggtcagc gctccaccgc gagacccgcg gacgtcggag
     2641 cgagctgggc gatgagcgac ccggaggaga ccacgtcgtg ggagcgcgtg cgacctcggg
     2701 aggtcaccga cgagcagtgg gatcgcttcg aggacgccgt cgcggagatg ttcgccgcct
     2761 tcggcatgga cctccagacc gaggggacac acaacacgcc gcgccggttc gtccgagcga
     2821 tgtacgaggc gacgacgggc tacgagggcg atccgaagct cttgaccgcg ttccccaccg
     2881 agtgtcgcgg gggccccgac tgttcgatca gccaggtgat cgaggggccg atcccgttct
     2941 tcgccctctg cgagcaccat gcgttcccgt tctacggcgt ggcgcacgtg ggatacgtgg
     3001 cgcacgagca gatcatcggg atctcgaaga tgacgaggct ggtccgggtg tacgcccgcc
     3061 gcttcacggt gcaggagcgc ctgggtcagc agatcgccga ccacctgaac gaggtgatgg
     3121 atccgcacgg cgtcgcggtg catctgcagg ccgcgcacct ctgcacccag atgcggggcg
     3181 tccgcgagga gcggtcgcgg acctccacct cgttctggcg cggcaactac gagcgggatc
     3241 ccgagatgcg ccgcgagttc ctcaccgtgg cgcagcgcga gtgaccgcgc tcgacgtcca
     3301 cctggaggcc gtcttcgacg ccggcggcgc tcccgacatc ccggtccccg aggagatcgt
     3361 ctcggcgtac ggggggtcgt tccggctccc cgaacgggtg gtctacgcga acttcgtgtc
     3421 ctcgatcgac ggcgtcgccg cgatcggggg cgtcgagatg tcctcggccg agatcagcgg
     3481 cggcggcgcg gccgaccgat tcgtgatggc gctcctgcgc gccgtggccg acgccgtcgt
     3541 ggtcggcgcc gggacggtcc gggagcacga cggtccctgg accgccgaga aggcgttccc
     3601 gccgggcgcc gagatcctct cccggctgcg agcggggatc tcggcgagcg agtcgccgac
     3661 gctcgtcgtc gtgaccgcga gcggggagct ccccgccgag catcccgcgc tcgcggacgc
     3721 gatcgtcgtc acgacgtcgg ctggcactcg gtcgctcgcc gagggcgggg ctcgggtcgg
     3781 ggaagtgatc gacgtcgggg acgcggatcg cgtcgatggc gccgtcgcga tcgatcgtct
     3841 ccgcgagcgc ggctatcagc ggatcctcac ggaaggcggg ccccggttga tgggcgcgat
     3901 gctggagtcc tctgccgtcg accagctgtt ccttacgatc tcgccgaagc tgatcggcgg
     3961 cggcccggag cgacccccac tcacggatga gaccgacctc ctcgaacggg aggtgggagc
     4021 gcgcttgctc tcgatccgtc gctccgacga ctacctcttc ctccggtacg gcctctcgtc
     4081 gccggactga cggggttccg gtcggcggtc cccggaaacg tcaccaggcc gcgagcccgc
     4141 ccacgatcgc tccgtagagg atgtgcgcga tcaggctgac gaccggtgtc ccggcgccgt
     4201 agttcagcat caggaacccg ggcgactcga tcagcgggga cgatcgcgcg tcggtccacg
     4261 gcctccccat ccgaggatgc acgaccggca ggaggacgtt cacgagggcc gtccccgcga
     4321 agagcccgtg cgcgagaccg aggagcgccc ccaggagcca cccgctcgcg tcgacgacga
     4381 ggaagaacac ggcgtaggcc agcgagaaga gcaggccgaa cccgaagtgc gcgacgtagc
     4441 cgatcgcctt cgcccgcgcc cggtccttgg tgacggcggt gccgagcagg aacgggagat
     4501 ccacgcgcgt gagccccagc tcgctcgcgg tccgcaacac ggtcgtgagc acgaaggtcc
     4561 cgagcagacc gcccgcaagg gcaccgagga cgctcacgtc cgacctcgac gcgcttcctc
     4621 gatcgcggtg gcggccccga tcagcgccag gtgcggcagc gcctgcggga agttcccgag
     4681 gaacgacccg tcggccggat cgatctcctc ggcgtagagc ccgacgtcgt tcgccatcgc
     4741 gatcagccgc tccatcagct gctcggcctc gtcgacccgc cccgcccgtg cccaggcgtc
     4801 cgcgagccag aaggagcacg cgaggaacgc gccctcctcg cccggcaggc cgtcgtccac
     4861 cacgtaccgc atcacgagcg gaccgttccg gaggtgccgg tcgatcgccg ccatcgtgcc
     4921 gagcatccgt tcaccggcgg gctccccgta ccccaggatc gaggcgtgca gcacgctcgc
     4981 gtcgaggtcc tccgagccgt cggagcggac gtagctgtgc agtcggtccg accagcatcg
     5041 ctcctccacg aacgcctcga tcgcgtcccg ttcgcgcacc catcgatcga cgcgatcgga
     5101 cggcacccgg gtcgaagccg ccagcgcgat cgctcggtcg agcgccaccc agcacatcat
     5161 cttcgactgg gtgtaatgcc ggggctcgct ccgcacctcc cagatcccgg agtctggtgt
     5221 ccgccagatc tcgcacacga ggtccgcgat ccgggcgagc cgg
//
LOCUS       DASUVE010000237         3096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_862999, whole genome shotgun sequence.
ACCESSION   DASUVE010000237 DASUVE010000000
VERSION     DASUVE010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862999"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /gene="obgE"
                     /locus_tag="VF036_05185"
     CDS             complement(<1..899)
                     /gene="obgE"
                     /locus_tag="VF036_05185"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX6264657.1"
                     /translation="MTFVDEVDVEVRAGRGGDGSAAMRSEPYKPKGGPEGGDGGRGGD
                     VVFRATRNLHDLAWFADHPHLRAEPGTPGARARRDGGNGKDLVVAVPDGTVVFDEQAL
                     IADLVGEGAEAVVARGGRGGRGNTRFAGPKNRVPRTAEAGEEGEERRLHVELRTVADL
                     GLVGLPNAGKSTLLAKLTAARPKIADYPFTTLTPNLGVADAGDDRFVVADIPGLVEGA
                     HEGKGLGHRFLRHITRCRALVLVVDLSSPDAPADLETVRAELRAYDPELAERPSLVVG
                     TKVDLVEDPARAADAIHGLPVSAV"
     gene            950..1399
                     /locus_tag="VF036_05190"
     CDS             950..1399
                     /locus_tag="VF036_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264658.1"
                     /translation="MVEPVTVWMVHLGLRSGTKPPEVRGTLTLGEEGLEFVERKSGAD
                     VRFDYASIRRAKRLRGSPVLLLDWTTGEAERKTAFYFSQPPPLEPVPRSATIDPRGPL
                     GPFTRATPSKRQVARMNLGYLRSTSVTNRQVVKAWAEAVSERMRRAG"
     gene            complement(1396..1650)
                     /gene="rpmA"
                     /locus_tag="VF036_05195"
     CDS             complement(1396..1650)
                     /gene="rpmA"
                     /locus_tag="VF036_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX6264659.1"
                     /translation="MAHKKGGGSSRNGRDSTSKRLGVKAFGGEAVTAGAIIVRQRGTR
                     IHPGDGVGKGGDDTLFALRDGTIEFHESRGRKYASVLADA"
     gene            complement(1670..2026)
                     /gene="rplU"
                     /locus_tag="VF036_05200"
     CDS             complement(1670..2026)
                     /gene="rplU"
                     /locus_tag="VF036_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX6264660.1"
                     /translation="MYAVIKTGGKQHRVQVGDVIEIEKLVVDGETVEFHPLLLVDDDG
                     KTHVGTEAAKAVVIAKPLGEKKGEKVKVFKYRPKSGYARKAGHRQLLTMLEITEVKVP
                     AKRTTAAKKDAAAASE"
     gene            complement(2064..>3096)
                     /locus_tag="VF036_05205"
     CDS             complement(2064..>3096)
                     /locus_tag="VF036_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX6264661.1"
                     /translation="VNYSPEDLEGGRPPRIERVLKSGQAVMVQVTKDPMGGKGARLTA
                     QISLPGRFLVLAPDQDVSGISRRLSDEARKRLKAILNKVKPKAHGVIVRTAAEGASEE
                     EITDDLERLVALWDQIQRTAKKVKAPDVLYEEPELTVRVVRDLFTDEEFRGLVTDSER
                     VHDKVMEYVRDVAPDLESKVTLHRGPLSAFEEYRIVEQIHKALDRKVWLPSGGYLIIE
                     QTEAMTIVDVNTGKAVGKTNLEETVLTTNLEAAKEVARQLRLRDIGGIIVIDFIDLLL
                     EQNKRKVEDTMRETLAIDKTRSQVFEIGPLGIMQITRKRVSGGLVDSFSETCPQCEGR
                     GIIVNYDAS"
BASE COUNT          432 a         1112 c         1017 g          535 t
ORIGIN      
        1 acggccgaca ccggcaggcc gtggatcgcg tcggcggcgc gcgccggatc ctcgacgagg
       61 tccaccttcg tccccaccac gagcgacggg cgctcggcga gctccggatc gtacgcacgg
      121 agctccgcgc ggacggtctc caggtcggct ggcgcgtccg gggacgagag gtccacgacg
      181 aggacgagcg ctcggcatcg ggtgatatga cggaggaagc ggtgccccag ccccttgccc
      241 tcgtgggcgc cctcgaccaa cccggggatg tcggcgacga cgaaccgatc gtcgcccgca
      301 tccgcgacac ccaggttggg cgtgagcgtg gtgaagggat agtccgcgat cttcggcctg
      361 gcggcggtca gcttcgcgag cagcgtcgac ttccccgcgt tcgggagtcc cacgaggccg
      421 agatcggcca ccgtccgcag ctccacgtgc aagcggcgct cctcgccctc ctcgccggct
      481 tcggcggtcc tgggcacgcg gttcttggga cccgcgaagc gtgtgttgcc gcgcccaccg
      541 cgtcctcccc tcgcgaccac ggcctcggcg ccctcgccga ccaggtcggc gatcaaggcc
      601 tgctcgtcga agaccaccgt gccgtccggc acggcaacga cgagatcctt gccgttccca
      661 ccgtcgcggc gggcccgagc tccgggggtg cccggctcgg cgcgcaggtg cgggtggtcc
      721 gcgaaccacg cgaggtcgtg caggttgcgg gtcgcgcgga acaccacgtc gccgccacgg
      781 ccaccgtcgc cgccctccgg tccacccttc ggcttgtacg gctcgctgcg catcgcggcg
      841 gacccgtcac caccgcgacc ggcgcgcacc tccacgtcga cctcgtccac gaacgtcatg
      901 ccgaacagcc taggtggtcg accgcgtccc cagccgcgaa gattgccgcg tggtcgagcc
      961 cgtcaccgtc tggatggtcc atctcggtct ccgatccggg acgaaaccgc ccgaggtgcg
     1021 gggaacgctc accctcggcg aggaaggact ggagttcgtc gaacgcaaga gcggggcgga
     1081 tgttcgcttc gactacgcct cgatccgtcg cgcgaagcgc ctccgaggct ctcccgtgct
     1141 cctgctggac tggacgacgg gtgaggcgga gcggaaaacg gccttctatt tctcgcagcc
     1201 gcctccgctg gaaccggtcc ctcgctccgc gaccatcgac ccacgggggc cgctcgggcc
     1261 gttcacgcgg gcgacgccgt cgaagcgtca ggtggcgagg atgaacctcg ggtatctccg
     1321 ctcgacgagc gtcacgaaca ggcaggtcgt gaaggcgtgg gcggaggcgg tgtccgagcg
     1381 gatgcggcga gccggctagg cgtcggcgag cacggacgcg tacttgcggc ctcgcgactc
     1441 gtggaactcg atcgtgccgt ctcgaagggc gaacagcgtg tcgtccccgc ccttgccgac
     1501 gccgtcgccg gggtggatcc gcgtcccgcg ttgacgcacg atgatcgcgc cggcggtgac
     1561 cgcttcacca ccgaacgctt tgacccccag tcgcttcgag gtcgagtccc gaccgttccg
     1621 ggacgagcca ccacccttct tgtgtgccat gatccgacga ggctaccagc tactcggagg
     1681 ccgccgcggc atccttcttc gcggcggtcg tccgcttcgc cggcaccttc acctcggtga
     1741 tctcgagcat cgtgagcagc tgccgatgcc cggccttgcg ggcgtagccg gacttgggcc
     1801 ggtacttgaa gaccttgacc ttctcgccct tcttctcgcc caagggcttg gcgatcacga
     1861 ccgccttcgc ggcctcggtc ccgacgtgcg tcttgccgtc gtcgtccacg aggagcaacg
     1921 ggtggaactc caccgtctcc ccgtcgacga ccagcttctc gatctcgatc acgtcgccga
     1981 cctgcaccct gtgctgcttg ccgccggtct tgatcaccgc atacatggct cgtttcctct
     2041 ctggcgtcgt tcggccgtgg tcgtcagctc gcgtcgtagt tcacgatgat gccccgcccc
     2101 tcgcactggg ggcaggtctc cgagaaggag tccaccagac cgccggagac gcgtttgcgg
     2161 gtgatctgca tgatccccag tgggccgatc tcgaacacct gggacctcgt cttgtcgatc
     2221 gcgagggtct cgcgcatcgt gtcctcgacc ttccgcttgt tctgctccag gagcagatcg
     2281 atgaagtcga tcacgatgat gccgccgatg tcccggaggc ggagctggcg cgcgacctcc
     2341 ttcgccgctt ccaggttcgt cgtgaggacc gtctcctcga ggttcgtctt gccgacggcc
     2401 ttccccgtgt tgacgtcgac gatcgtcatg gcctcggtct gctcgatgat caggtagcca
     2461 cccgacggga gccagacctt cctgtccaac gccttgtgga tctgctccac gatgcggtac
     2521 tcctcgaacg cggacagggg accacggtgc aacgtgacct tcgactcgag gtcgggcgcc
     2581 acgtcgcgga cgtactccat caccttgtcg tggacgcgct cggagtcggt gaccagacct
     2641 cggaactcct cgtccgtgaa gaggtcccgg accacgcgga ccgtgagctc gggctcctcg
     2701 tacaggacgt cgggcgcctt gaccttcttg gcggtcctct ggatctggtc ccagagcgcg
     2761 accaggcgct cgaggtcgtc ggtgatctcc tcctcggagg ccccctcggc ggcggtccgg
     2821 acgatcacgc cgtgcgcctt cggcttcacc ttgttcagga tcgccttcag cctcttgcgc
     2881 gcctcgtccg agagccggcg ggagatgccc gagacgtcct ggtcgggtgc gaggacgagg
     2941 aaccggccgg gcagcgagat ctgcgcggtg aggcgcgcgc ccttcccacc catcggatcc
     3001 ttcgtgacct gcaccatgac ggcctgcccg gacttcagca cacgctcgat ccggggcggc
     3061 cggccgcctt cgagatcctc gggcgagtag ttgacc
//
LOCUS       DASUVE010000238         4321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_676294, whole genome shotgun sequence.
ACCESSION   DASUVE010000238 DASUVE010000000
VERSION     DASUVE010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676294"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VF036_05210"
     CDS             complement(<1..547)
                     /locus_tag="VF036_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX6264662.1"
                     /translation="MADEHPEIDFANPDSFVEGVPYGWFDHLRTEHPVFWHPERSPNQ
                     GFWAVTRYDDLTAIHMDWRTYSSELGAVSLEELDDEQLEIRKSMLETDPPRHTELRAI
                     CSKRFSARGVGKYEDWIRDVARDVLDRALPMGEFDFVPEISRDLPIRFLCSIFTVPQE
                     DAPQLISWGDQMIANQDPDLSR"
     gene            complement(594..1037)
                     /locus_tag="VF036_05215"
     CDS             complement(594..1037)
                     /locus_tag="VF036_05215"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6264663.1"
                     /translation="MVNLSIEIDAPPERVFRHLADVATHPGWANPKAAMTMEQTAGDG
                     PGPDSACRSSGVFVGKPVTADISVTVYEPPRRFAIRSDQHQEGKRDVWYVNDYTLTPA
                     DTGTRLSKRVTSNGNPAIFYLAYPAIRSDQMTSLRNLKRLAESGG"
     gene            complement(1147..2448)
                     /locus_tag="VF036_05220"
     CDS             complement(1147..2448)
                     /locus_tag="VF036_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX6264664.1"
                     /translation="MDLSGRRLRLLISDILGLERGKYLFGDVAEAGHAAFCIGLYPLT
                     TDKEILPVRRQHFDVGLPDVEAQMDRDTLRPGWEDDTVIGIADVTQHGVPLEVDPRYV
                     LKQAVRAWREMDLEPQIAFELEFYLCERVDGAWAPADLPSHRVYGTGMSVDPSGAVHD
                     MVTASLGSGFHVESWCSEFDSAAFEVNWRYRDAIAGADEAFLFRVLVREIAARHGRHA
                     TFLGRPFNDRGGCGMHVNLSFRRRDGSNAFHDPDDPDGLSDLARKSIAGLLAHHRGIS
                     AICAPTTNAYKRLKPDMLNGYLANWGFDDRTVGVRVPPARGEGSRIEHRMADAAANPY
                     LVGAALLHAARLGVTEELELGPAQEPGADPNTDVAVPNTLEEALEELRKDTKLAAEMG
                     EWLVESFVALKQAEWDRYVASGETSDTAVTSWELDYYLPFF"
     gene            complement(2512..3357)
                     /locus_tag="VF036_05225"
     CDS             complement(2512..3357)
                     /locus_tag="VF036_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6264665.1"
                     /translation="METRRILLDGVATTVTREGDELVAPDGRRIPMDEPTHLPPIQPT
                     KMVCVHLNYESRRAEFLAELGPAPTFFHKPTTSLNVHGGEVVRPARCRYLNYEGEIAI
                     VIGERCSHIAPEEADEYIAGYTVANDYGLHDFRDTDAGSMLRVKGSDTLCPLGPGLVT
                     DWDFHGKRIQTRVNGEVVQDGTTDEMIWDMRYLVADIARTITLLPNDVILSGTPAHSR
                     PVQPGDVVEVEVDGLGILRNFIVEGAVPVREDVGAQPSGSEEVISTAFGGDWEFRGVR
                     APRRG"
     gene            3378..4286
                     /locus_tag="VF036_05230"
     CDS             3378..4286
                     /locus_tag="VF036_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264666.1"
                     /translation="MRWVVGTAEGLVGSEGERWLADRAITALAWDGHGWLVLVETREV
                     LRVHGDEVETLGEIDDETGRCLLPTADGVLLGTAHARLGVLRDGAVTMIRSFDDSEGR
                     SEWYTPWGGPPDTRSLSASADGGLFANVHVGGILHAEVPDGVWSPTIDIDADVHQVLA
                     DPLDASHVVAATARGLAETHDTGATWHFAAEGLHAPYARAVALDGDRLFMSASTGPRG
                     GRAALYRREPGADVFERCADALPDWFPGNIDSHCLAAGGGTIAFGAEDGRVFVSGDGG
                     GSWREIPGGKARVTCVGVAASEGRGT"
BASE COUNT          658 a         1442 c         1521 g          700 t
ORIGIN      
        1 cgcgcgagag atccgggtcc tggttcgcga tcatctggtc gccccacgag atcagctgcg
       61 gcgcgtcctc ctgcggcacc gtgaagatcg agcagaggaa ccggatcggc aggtcgcggc
      121 tgatctcggg cacgaagtcg aactcgccca tcgggagtgc ccgatccagg acgtcgcgtg
      181 ccacgtcgcg gatccagtcc tcgtacttgc caaccccacg cgccgagaac cgcttcgaac
      241 agatcgcgcg gagctccgtg tgccggggag gatcggtctc gagcatcgac ttccggatct
      301 cgagctgttc gtcgtcgagc tcctcgaggg agacggcgcc gagttccgag gagtaggtcc
      361 gccagtccat gtggatcgcg gtgaggtcgt cgtaccgggt caccgcccag aagccctgat
      421 tgggcgaacg ctccggatgc cagaagaccg ggtgctcggt gcggaggtga tcgaaccatc
      481 cgtacgggac cccttcgacg aagctgtccg ggttcgcgaa gtcgatctcg ggatgctcgt
      541 ccgccatcgg gcgcatcata cgggcgctcc ggcggagccc ggccgggcgg aggtcacccg
      601 ccggattcgg cgagccgttt caggttgcgg agcgaagtca tctgatccga gcggatcgcg
      661 gggtacgcga gatagaagat cgccgggttg ccgttcgacg tgacccgctt cgagaggcga
      721 gtcccggtgt ccgccggcgt gagcgtgtag tcgttcacgt accagacgtc ccgcttgcct
      781 tcctggtgct ggtcggagcg gatcgcgaat cggcgcggcg gctcgtagac cgtaaccgag
      841 atgtcggcgg tcacgggctt gccgacgaag acgcccgagg agcggcacgc ggagtcgggc
      901 ccggggccat cgccggcggt ctgctccatc gtcatcgcgg ccttcgggtt cgcccacccc
      961 ggatgcgtgg cgacgtcggc aaggtgtcgg aacacgcgct ccggcggcgc gtcgatctcg
     1021 atcgagaggt tgaccactgg gttccctcct ccacctctgc ggtggaggat ttaagctacg
     1081 acttacatat tgtcaagggc gtgatcagga gaagggctca gacgaagtgc gtggaagagg
     1141 aagggcctag aagaagggaa ggtagtaatc gagctcccac gacgtgaccg cggtatcgga
     1201 ggtctcgccg gacgcgacgt accgatccca ctccgcctgc ttcagcgcca cgaacgactc
     1261 cacgagccac tcgcccatct ccgcggcgag cttcgtgtcc ttccggagct cctcgagcgc
     1321 ctcctcgagc gtgtttggca cggccacgtc cgtgttgggg tcggcaccgg gctcttgcgc
     1381 ggggccgagc tccagctcct cggtcacccc gagccgcgcg gcgtggagca gggccgcgcc
     1441 caccagatac gggttggccg cggcatcggc catgcggtgc tcgatcctcg acccctctcc
     1501 gcgggcgggc gggacacgga cgcccacggt ccgatcgtcg aatccccaat tcgccaggta
     1561 accgttcagc atgtccggct tcagccgctt gtaggcgttc gtggtgggcg cgcagatggc
     1621 ggagatgcct cggtgatggg cgaggaggcc ggcgatcgac ttcctggcca gatccgacag
     1681 gccgtcggga tcgtccgggt cgtggaacgc gttcgaaccg tcccgccgcc ggaacgagag
     1741 gttcacgtgc atcccgcagc cgccgcgatc gttgaacggc ctgccgagga acgtcgcatg
     1801 tcggccgtgt cgggcggcga tctcgcggac gagcacgcgg aacaggaacg cctcgtcggc
     1861 tcctgcgatc gcgtcgcgat agcgccagtt gacctcgaac gcggcgctgt cgaactcgct
     1921 gcaccagctc tccacgtgga agcccgaccc cagcgatgcg gtcaccatgt cgtgcacggc
     1981 gccgctcggg tcgacggaca tgcccgtgcc gtagacacga tgcgacggga gatcggcggg
     2041 cgcccacgcg ccgtccaccc gctcgcagag atagaactcg agctcgaacg cgatctgggg
     2101 ttcgagatcc atctcgcgcc aggcccgcac ggcctgcttc aacacgtagc gagggtcgac
     2161 ctcgaggggc acgccgtgct gcgtgacgtc ggcgatgccg atcacggtgt cgtcctccca
     2221 ccccggtcgg agggtgtccc gatccatctg cgcctcgacg tcggggaggc cgacgtcgaa
     2281 gtgctggcgg cgaaccggca ggatctcctt gtccgtcgtg agcggataca gcccgatgca
     2341 gaacgccgcg tgaccggctt cagcgacgtc gccgaacaga tacttcccgc gctcgaggcc
     2401 caagatgtcc gagatgagca accgcaggcg tctgccgctc agatccatgt cgcgcgcctc
     2461 ctcgtatggc aaccaggacc gtatccgatg acggcgcgat ccgccccagg ctcagccgcg
     2521 cctcggagcc ctgacccccc ggaactccca gtctccgccg aacgcggtcg agatcacctc
     2581 ttcggacccg ctcggctgag cgcccacgtc ctcgcggacc ggcacggcac cctcgacgat
     2641 gaagttgcgg aggatcccga ggccgtccac ctcgacctcg acgacgtcgc ccggttggac
     2701 ggggcgggag tgggcggggg tgcccgacag gatcacgtcg ttgggcagga gcgtgatcgt
     2761 gcgcgcgatg tcggcgacga ggtagcgcat gtcccagatc atctcgtcgg tggtgccgtc
     2821 ctgcaccacc tcgccgttca cccgcgtctg gatgcgcttg ccgtggaagt cccagtccgt
     2881 cacgaggccg ggaccgagcg ggcagagcgt gtcggagcct ttcacgcgga gcatcgagcc
     2941 ggcgtcggtg tctcggaagt cgtggaggcc gtagtcgttc gcgacggtgt agcccgcgat
     3001 gtactcgtct gcctcttcgg gagcgatgtg cgagcaccgc tcgcctatca cgatcgcgat
     3061 ctccccctcg tagttcaggt accggcatcg cgccgggcgc accacctcgc cgccgtgcac
     3121 gttcaacgac gtggtgggtt tgtggaagaa cgtgggtgcc ggacccagct cggcgaggaa
     3181 ctccgcccgc cggctctcgt agttgaggtg gacgcacacc atcttcgtgg gctggatcgg
     3241 agggaggtgg gtcggctcgt ccatcgggat ccggcggccg tcgggagcga cgagctcatc
     3301 accttctcgc gtgacggtgg tcgccacacc gtccaggagg atccgccgcg tctccatcca
     3361 acgatagcct agaggtcgtg cgctgggtcg ttggaaccgc ggaggggctc gtcggaagcg
     3421 agggcgagcg ctggctcgca gatcgagcca tcaccgcgtt ggcgtgggac ggccacgggt
     3481 ggctcgtgct cgtcgaaact cgcgaggtgc tgcgcgtcca cggagacgag gtcgagacac
     3541 tcggtgagat cgacgacgaa accggacgct gtctcctgcc gaccgccgac ggggtgctgc
     3601 tcggcacggc gcatgcgcgg ctcggcgtgc ttcgcgacgg cgccgtcacg atgatccgct
     3661 cgttcgatga ctcggagggg cggagcgagt ggtacacgcc gtggggcggc cctccggaca
     3721 cgcggtcgct ctccgcgtcg gccgacggcg gcctcttcgc caacgtccac gtcggcggga
     3781 tcctccatgc ggaggtcccc gacggcgtct ggtcaccgac gatcgacatc gacgccgacg
     3841 tgcatcaggt cctcgcggat cccctcgacg cttcccacgt cgtcgccgcg acggcgcgcg
     3901 ggctcgccga gacccacgat acgggcgcca cctggcactt cgccgcggag gggctccacg
     3961 ctccgtacgc gcgcgcggtc gctctcgatg gggaccgact gttcatgagc gcctccacgg
     4021 gtccccgtgg cggccgggcg gcgctctacc gtcgggagcc gggagcggat gtgttcgagc
     4081 ggtgtgcgga tgccctgccg gactggttcc cggggaacat cgacagtcac tgcctcgccg
     4141 cgggcggggg cacgatcgcg ttcggagccg aagacggccg ggtgttcgtg tccggcgacg
     4201 gcggagggtc ctggcgcgag atccctggcg ggaaggcacg cgtgacgtgt gtcggcgtgg
     4261 ccgcctcgga gggacgcggc acgtagactc cgcatccgtg gccgagctga aggggttcct
     4321 c
//
LOCUS       DASUVE010000239         3230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1431600, whole genome shotgun sequence.
ACCESSION   DASUVE010000239 DASUVE010000000
VERSION     DASUVE010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431600"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1771)
                     /locus_tag="VF036_05235"
     CDS             complement(<1..1771)
                     /locus_tag="VF036_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX6264667.1"
                     /translation="MATKTSPSRRSGGSGSRSRPSKKPAPRKSRSKSRKPSKTPVRTV
                     LSPWARDALGIGLIVLSLLAALSLWLEAGGVVGRGITWFIRGSFGIGAYAFPVLGAWW
                     GFVLLRDIGREDRVRMAIGFAVLVAGVLGLISLFAGNPSITEGWTGDESLSEAGGAIG
                     ALAAWPLAKVISPIGAAIVCAGLASLGLLIFTGTPFAAVKEKFDSFREERDERDPVAA
                     RAKDSDHAKPRRRWSLREAFGLTDDVIHLPEADDGQEAETETVASVPQDLAFPSEPAL
                     PATSGSRSRTVRTPFGPYQLPPPDLFRTAPPSNADSRAEKDTLAALERTLHTFGVDAQ
                     VVGAHRGPTVTMYEVAVASGTKVSKVVALSSDISYALASPDVRIQAPIPGKSAIGIEV
                     PNTHRDFVMVGDVLRSPAAKQATHPLEVALGKDVHGRARMVNLAEMPHLLIAGATGAG
                     KSSLVNSFITSLLARTTPEDVRLILVDPKRVELSHFGDVPHLLSPVIVHPKRAAEALQ
                     WIVREMEMRYELLATVAVRDIDSYEEGLRDGTLRIPPGMEEKFEHMPFIVVVIDELAD
                     LMMVAPRDVEDAICRIAQMARAVG"
     gene            complement(1821..>3230)
                     /locus_tag="VF036_05240"
     CDS             complement(1821..>3230)
                     /locus_tag="VF036_05240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX6264668.1"
                     /translation="DRRLEVYGTPFTLALLQGKLEEHEVEHLVDLRTVSPGELASTGP
                     FTMRFLRVTHSIPDGMAVVVDTPFGSILHTGDFKIDQTPLDGRATDLHGLAQEAGRGK
                     GVHLLLSDSTNAEEPGYSVSERTVGPVLLDIVENAGGLVVAACFSSHIHRVQQIVNAA
                     RANERVISFLGRSMLKSVDAARRLGHLHVDDKDVVDISEIDRLDPGRVVVICTGSQGE
                     PYSALSLMAQRQHKWVTIGPGETVVLSSSLIPGNEPAIHRVVDSLYRTGADVLHMPSD
                     PVHASGHAAQEELRLMLSLVRPRWFIPVHGERRHLQHHARLAHEVGIPPERTLVCEDG
                     DVVEVADEVRHVDRIQAGMTFVDGLGIGDVGGEVLRDRRKLSADGVVVVVIIVDSQTG
                     ELVGGPDLVNRGFVHEESSADILEEGRLRVIEAVKESADARVTDPSALQQNVRRVLKR
                     YFNEATQRKPVILPVIMEV"
BASE COUNT          534 a         1107 c         1104 g          485 t
ORIGIN      
        1 tcccgacggc acgcgccatc tgggcgatcc ggcagatcgc gtcctccacg tcgcgcggcg
       61 ccaccatcat caggtccgcg agctcgtcga tcacgacgac gatgaacggc atgtgctcga
      121 acttctcctc catgccgggg gggatccgga gcgtgccgtc gcggagcccc tcctcgtagg
      181 agtcgatgtc gcggacggcg accgtggcga ggagctcgta ccgcatctcc atctctcgga
      241 cgatccactg caaggcctcc gctgcccgct tcggatggac gatcaccggg gagagcaggt
      301 gcggcacatc gccgaagtga ctgagctcca cacgcttcgg gtcgaccagg atcagccgga
      361 cgtcctccgg cgtcgtgcgc gcgagcaacg aggtgatgaa ggagttcacg agcgaggact
      421 tcccggcgcc ggtggcgccg gcgatcagca gatgcggcat ctccgcgagg ttcaccatcc
      481 gcgcgcggcc atggacgtcc ttgccgaggg cgacctcgag gggatgggtc gcctgcttcg
      541 cggccggaga acgcaacaca tcgcccacca tcacgaagtc ccgatgggtg ttcggaacct
      601 cgatcccgat cgccgatttg cccgggatcg gcgcctggat ccggacgtcc ggggaggcga
      661 gcgcgtacga gatgtcgctc gagagggcga cgaccttgct caccttggtt cccgaagcca
      721 ccgcgacctc gtacatcgtg acggtgggac cgcggtgcgc ccccacgacc tgggcgtcga
      781 cgccgaacgt gtgcagtgtc ctctcgagtg cggccagcgt gtccttctcc gcgcgcgagt
      841 ccgcgttgga gggaggcgcc gtccggaaca ggtccggcgg gggaagctgg tacggcccga
      901 acggtgtcct gacggttcgc gaacgagagc cgctcgtcgc cggcagcgcc ggctcggagg
      961 ggaacgcaag gtcctgaggg accgacgcga ccgtctcggt ctcggcctct tgcccgtcgt
     1021 ccgcctcggg gaggtggatc acgtcgtcgg tgagcccgaa cgcctcgcgg agcgaccatc
     1081 ggcggcgcgg cttcgcgtga tccgagtcct tcgctctcgc ggccaccggg tcgcgttcgt
     1141 cgcgctcctc acggaacgag tcgaacttct ccttgacggc ggcgaacggc gtgccggtga
     1201 agatcagcag tccgaggctc gcgagcccgg cgcacacgat cgcggcaccg atcggagaga
     1261 tgaccttcgc gagcggccac gcggcgagcg ccccgatcgc cccaccggcc tcggacaggc
     1321 tctcatcgcc cgtccatccc tccgtgatgc tggggttgcc ggcgaacaag gagatgaggc
     1381 cgaggacgcc ggcgacgaga acggcgaacc cgatcgccat gcgcacgcgg tcctcgcggc
     1441 cgatgtctcg caggagcacg aacccccacc acgcaccgag caccgggaag gcgtaggcgc
     1501 cgatgccgaa actcccgcgg atgaaccagg tgataccacg accgacgaca ccaccagcct
     1561 cgagccacag ggacagggcg gcgaggagcg ataggacgat cagcccgata ccgagggcat
     1621 cccgcgccca tggcgacagg accgtcctga cgggggtctt ggacggcttc ctgctcttac
     1681 ttcggctctt ccgaggggca ggcttcttgg agggacgcga acgcgacccg ctgcccccgg
     1741 acctccggga gggggaagtc ttcgttgcca tgtgcggaca agcatgactg ctgaggcgga
     1801 cgcttcaagg acccggaggg tcagacctcc atgatcacgg gcaagatcac gggcttccgt
     1861 tgggtcgcct cgttgaagta ccgcttgagg acgcgacgga cgttctgctg caacgcggac
     1921 gggtcggtca cccgggcgtc cgccgactcc ttcaccgcct cgatcacgcg caggcggccc
     1981 tcctccaaga tgtcggctga ggactcctcg tgcacgaagc cgcggttcac gaggtcgggg
     2041 ccgcccacca gctcgccggt ctgcgaatcg acgatgatga ccaccacgac caccccgtcg
     2101 gccgagagct tccgccggtc ccggagcacc tccccaccca cgtcgccgat cccgagaccg
     2161 tccacgaacg tcatccccgc ctggatgcgg tcgacgtggc gcacctcgtc ggcgacctcc
     2221 acgacatcac cgtcctcgca gacgagcgtc cgctcgggcg ggatgccgac ctcatgggcc
     2281 aggcgcgcgt gatgctgcag gtgtcggcgc tcgccgtgga ccgggatgaa ccaccgcggc
     2341 cggaccagcg agagcatcag gcgtagctcc tcctgcgctg catgtccgga ggcgtggacc
     2401 ggatcggacg gcatgtggag cacgtcggcg cccgtccggt agagggagtc gacgacgcga
     2461 tggatcgcag gctcgttccc ggggatcaac gacgacgaca ggaccacggt ttcgccgggg
     2521 ccgatcgtga cccacttgtg ctgtcgttgc gccatcagcg agagggccga gtagggctcc
     2581 ccttgcgagc cggtgcagat caccaccaca cggccgggat ccagtcggtc gatctccgag
     2641 atgtcgacga catccttgtc gtcgacgtgg aggtgaccga gtcgccgcgc ggcatcgacg
     2701 gacttcagca tggagcgacc gaggaacgag atgacccgtt cgttcgcgcg cgccgcgttc
     2761 acgatctgct gcacgcgatg gatgtggctc gagaagcagg ccgcgaccac gaggccccct
     2821 gcgttctcga cgatgtcgag caggaccggc ccgacggtgc gctcgctgac ggagtaccca
     2881 ggctcctcgg cgttcgtgga gtcggaaagc agcagatgga cgcccttgcc gcgtcctgcc
     2941 tcctgggcca ggccgtggag gtccgtcgcc ctgccgtcga gcggagtttg gtcgatcttg
     3001 aagtcgcccg tgtggaggat cgagccgaag ggcgtgtcga ccacgaccgc catcccatcg
     3061 gggatcgagt gtgtgacgcg aaggaaccgc atcgtgaacg ggcccgtcga cgccagctcc
     3121 cccggcgaga cggtcctgag atcgacgagg tgctccacct cgtgctcctc gagcttgccc
     3181 tgcagcaacg cgagcgtgaa cggcgtgccg tagacctcga gacggcggtc
//
LOCUS       DASUVE010000240         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1432189, whole genome shotgun sequence.
ACCESSION   DASUVE010000240 DASUVE010000000
VERSION     DASUVE010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432189"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /gene="truB"
                     /locus_tag="VF036_05245"
     CDS             complement(<1..654)
                     /gene="truB"
                     /locus_tag="VF036_05245"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX6264669.1"
                     /translation="MTPVEGLLLVDKPAGITSHDVVHSVRRTLATKKVGHAGTLDPMA
                     TGLLLIGVGRATRLLRFLGDLAKTYEGTLRLGVETDTLDADGDVVRESPVDVTNEQVA
                     EAMRSLVGKSLQRPPAYSAVKVGGRKLYEAARAGEHLEAEPRAIRVDAFDPLSFDPPD
                     VTFRVICSGGTYVRVLAADVGAAVSTGAHLTALRRTAIGPYRAQDAVTPDAVREPLPI
                     "
     gene            complement(651..1646)
                     /locus_tag="VF036_05250"
     CDS             complement(651..1646)
                     /locus_tag="VF036_05250"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX6264670.1"
                     /translation="MPDGSEREGLRRAAEILSGADEVAIACHLNPDADALGSMLGLSN
                     FLRARGTRTVGSFANEPFELPRWIAFLPGADRLVPPTRFPAAPGVMVTCDCASFDRLG
                     VLGEAASRAGELIWIDHHRSNDGFGTVRLVDPDASSTCEMVFRLIETMSGEFDRDVAV
                     CLYAGLVTDTGRFQYQATTPETLRIAARLREEDFDHAGLVRAMYEDNDPGFLRLLGTA
                     LGRMTEVPEASVVWTYLTQADLADAGVAAGETDDVIDVLRTARDVDVAAVLKQQRDGR
                     FKVSVRSRGGHDLSAVASTFGGGGHRLAAGYTSEHGPEGTIERLVAALRQAPVPS"
     gene            complement(1639..1998)
                     /gene="rbfA"
                     /locus_tag="VF036_05255"
     CDS             complement(1639..1998)
                     /gene="rbfA"
                     /locus_tag="VF036_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX6264671.1"
                     /translation="MTQGARTARVAEEFREVLAEEIQKLKDPRIGFVTVTGVQVTADL
                     RRAWVSYTVLGDDKQQRSTAAGLRSATPHLRQALGRQVRLRNLPELEFQVDQTLAQHQ
                     HIEEIIERLHREGRSDA"
     gene            complement(2023..>2278)
                     /locus_tag="VF036_05260"
     CDS             complement(2023..>2278)
                     /locus_tag="VF036_05260"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX6264672.1"
                     /translation="ACGSLKEKRHVIRTLANGIRSTFQVAVAETGYQDMWQRAELGVA
                     AVGSEGHHLKKVMHGVERFVERWAEVDIIDIDLTLHSPED"
BASE COUNT          345 a          843 c          747 g          343 t
ORIGIN      
        1 gatcggcaag ggctcacgca ccgcgtccgg cgtgaccgcg tcctgtgccc ggtagggacc
       61 gatcgccgtg cgacggagcg ccgtcaggtg cgctcccgtc gacaccgccg cacccacgtc
      121 ggcggcgagc acccgcacgt aggttccccc ggagcagatc acgcggaacg tcacgtccgg
      181 tggatcgaac gacagggggt cgaaggcgtc gacccggatc gcgcgcggct cggcctcgag
      241 atgctcgccc gcccgcgcgg cctcgtagag cttccggccg cccaccttga ccgcgctgta
      301 cgcgggcggc cgctggaggg acttccccac gagcgagcgc atcgcctcgg cgacctgctc
      361 gttcgtcacg tcgaccggcg actcgcgcac gacgtcaccg tcggcatcca gcgtgtccgt
      421 ctcgaccccg agccggagcg tcccctcgta ggtcttcgcg agatcgccga ggaagcggag
      481 cagacgggtc gcgcgaccga ccccgatcag cagcagcccg gtcgccatgg gatcgagcgt
      541 ccccgcatgc cccaccttct tcgtggcgag ggttcggcgc acgctgtgga cgacgtcatg
      601 cgaggtgatc cccgccggct tgtcgacgag gagcaggccc tcaaccgggg tcacgatgga
      661 acgggcgctt ggcgtaaggc ggcaacgagc cgctcgatcg tcccctcggg cccgtgctcc
      721 gacgtgtagc cggccgcgag cctatgcccg ccgcccccga acgtcgaggc aacggcggaa
      781 aggtcgtgcc cgccgcggga gcgcacgctc accttgaatc gcccgtcccg ctgctgcttc
      841 agcacagcgg ccacgtccac gtcgcgtgcg gtgcggagca cgtcgatcac gtcgtcggtc
      901 tcccccgccg cgacgccggc gtcggcgaga tccgcctgcg tgaggtaggt ccagaccacg
      961 ctcgcctcag gtacttcggt catgcggccg agcgccgtcc cgagcaagcg gaggaagccc
     1021 gggtcgttgt cctcgtacat cgcgcggacg agcccggcgt ggtcgaagtc ctcctcgcgc
     1081 agccgagcgg cgatccggag cgtctccggg gtggtcgcct ggtactggaa tcgcccggtg
     1141 tccgtgacca aacccgcgta gagacagacc gcgacgtccc gatcgaactc gccgctcatc
     1201 gtctcgatca ggcggaagac catctcgcac gtggacgacg cgtcgggatc cacgaggcgg
     1261 accgtgccga aaccgtcgtt cgagcggtgg tgatcgatcc agatcagctc gccggctcgc
     1321 gaggccgcct ctccgaggac acccaaccgg tcgaacgaag cgcagtcgca cgtcaccatg
     1381 acgcccggag ccgccgggaa tctcgtcgga gggacgagtc gatccgcgcc gggaaggaag
     1441 gcgatccacc gcggcagctc gaacggctcg ttcgcgaacg agcccaccgt ccgcgtgccc
     1501 cgcgctcgca ggaagttcga gagcccgagc atcgacccga gcgcgtccgc gtccgggttc
     1561 aggtggcagg cgatcgccac ctcatcggct cccgacagga tctccgccgc ccggcggagc
     1621 ccttcgcgct cgctcccgtc aggcatcgga tcgtccctcg cgatgcagac gctcgatgat
     1681 ctcctcgatg tgctgatgct gcgcgagcgt ctggtccacc tggaactcca gttccgggag
     1741 gttgcgcagc cggacctgcc gaccgagcgc ctgacggagg tgcggcgtcg ccgaccggag
     1801 ccccgcagca gtcgatcgct gctgcttgtc gtctccgagg accgtgtagg agacccacgc
     1861 ccgccggagg tccgccgtca cctggacgcc ggtgaccgtg acgaagccga tccgagggtc
     1921 cttgagcttc tggatctcct cggcaaggac ctcgcggaac tcctcggcca cccgagcggt
     1981 ccgggctccc tgcgtcatgt ctgcatcccg ccttctacgg tctcagtcct ccggcgaatg
     2041 gagcgtgaga tcgatgtcga tgatgtcgac ctccgcccac cgctccacga agcgttcgac
     2101 gccgtgcatc accttcttga ggtggtggcc ctccgacccg acggccgcca cgcccagctc
     2161 cgcacgctgc cacatgtcct gatatcccgt ctcggccacc gccacctgga aggtggaccg
     2221 gatcccgttc gcgagggtcc tgatcacgtg acgcttctcc ttcaacgatc cgcacgcc
//
LOCUS       DASUVE010000241         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_679064, whole genome shotgun sequence.
ACCESSION   DASUVE010000241 DASUVE010000000
VERSION     DASUVE010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679064"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..>2491
                     /gene="carB"
                     /locus_tag="VF036_05265"
     CDS             62..>2491
                     /gene="carB"
                     /locus_tag="VF036_05265"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017576786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX6264673.1"
                     /translation="MSKRTDLSSILVIGSGPIVIGQACEFDYSGTQACRVLRREGYRV
                     TLVNSNPATIMTDPEFADRTYLEPLTPESVLAVIERERPDALLPTIGGQTGLNVSVAL
                     AESGNLDRMGVELIGASLETIHRAEDRSGFKEAMTKAGLDVPRAGIAHTLEEALQLGH
                     QLGFPVIVRPSFTLGGGGSGFAASHGELEGLARRSLEASPVSEVLVEESIAGWKEFEL
                     EVMRDHADNAVIVCSIENVDPMGVHTGDSVTVAPQQTLTDREYQAMRDDAIACLRAIG
                     VETGGSNVQFAVDPQTGRRVLIEMNPRVSRSSALASKATGFPIAKIAALLAVGYRLDE
                     IRNDITGATPASFEPTLDYVVVKIPRFAFEKFPEADARLTSTMKSVGEVMAIGGTFKE
                     AWGKAWRGLERSGADLGATYATAHDDVLGALRDGTEWRFHLIDRAFAAGHSVGEIAAA
                     SAIDPWFVDLFAQVYERAEGLAGAMGPSLGREILLDAKRIGLSDARIARETGSTERAI
                     RDRREQVGVVPVFKSVDTCGGEFPARTPYLYSTYDEESEVEPVSRPRVVILGAGPNRI
                     GQGIEFDYACVHAAFALEEAGFESVMVNSNPETVSTDYDTSSRLYFEPLSAEDVLAVC
                     RAERPAGVIAQLGGQTPLELARTLQDAGFPILGTSPDAIDLAEDRGRFAELLHEMDIP
                     APPHGEARTIDEARIVARRIGYPVVVRPSYVLGGRAMEIVYDDHELETFVRTAAEASR
                     EHPVLVDRFLEGAIEVDVDAVCDGVDVFIGAVMEHIEEAGVHSGDSSCQIPPATLSDE
                     ELDRIEDITRRL"
BASE COUNT          396 a          827 c          885 g          383 t
ORIGIN      
        1 atgcgcacga gggctcgagc agcgagcgtg cgagcgagag cccccgaagg gatggcccgt
       61 gatgtcgaag cggaccgacc tgtcctcgat cctcgtgatc ggttcagggc cgatcgtgat
      121 cgggcaggcg tgtgagttcg actattccgg gacgcaggca tgccgtgtcc tccggcgcga
      181 gggttaccgc gtcaccctcg tcaactcgaa ccccgcgacg atcatgacgg acccggagtt
      241 cgccgatcga acctacctcg agccgctcac gcccgagtcg gtgctcgccg tgatcgagcg
      301 ggagcggccc gatgccctgc tcccgacgat cggcggtcag accggcctga acgtgagcgt
      361 ggccctcgcc gagtcgggca acctcgaccg catgggcgtc gagctgatcg gcgccagcct
      421 cgagacgatc caccgcgccg aggaccgcag cggcttcaag gaggcgatga cgaaggcggg
      481 gctcgacgtc ccgcgggccg ggatcgcgca cacgctcgag gaagcgctgc agctcggcca
      541 ccagctcggc ttcccggtga tcgtgcgccc ctcgttcacg ctcggcgggg gagggagcgg
      601 gttcgcggcc tcgcatgggg agctcgaggg cctcgcacgc cgctcgctcg aagccagtcc
      661 ggtctccgag gtcctcgtgg aggagtcgat cgccgggtgg aaggagttcg agctcgaggt
      721 gatgcgcgac catgccgaca acgcggtgat cgtctgctcg atcgagaacg ttgatccgat
      781 gggcgtccac accggcgact ccgtcaccgt cgcgccgcag cagaccctga cggaccgcga
      841 gtaccaggcg atgcgcgatg acgcgatcgc gtgccttcgc gcgatcggcg tcgagaccgg
      901 cgggtcgaac gtgcagttcg cggtcgatcc gcagaccggg cgacgggtgc tgatcgagat
      961 gaatcctcgc gtgagccgat cgtcggcgct cgcatcgaag gccacgggat tcccgatcgc
     1021 gaagatcgcc gcactgctcg ccgtcggcta tcgactggac gagatccgca acgatatcac
     1081 gggcgccacg cccgcgtcat tcgagccgac gctcgactac gtcgtggtga agatcccgcg
     1141 gttcgccttc gagaagttcc cggaggccga tgcgcggctc acttccacga tgaagtccgt
     1201 gggcgaggtg atggcgatcg gaggaacctt caaggaggcg tgggggaagg cgtggcgcgg
     1261 gctcgagagg tccggcgccg acctcggagc gacgtacgcg acagcacatg acgacgtgct
     1321 cggcgccctc cgcgacggca ccgagtggcg attccacctg atcgatcggg cgttcgccgc
     1381 cgggcacagc gtgggcgaga tcgcggcggc ctcggcgatc gatccctggt tcgtcgatct
     1441 gttcgcccag gtgtacgagc gcgccgaggg actcgccgga gccatgggcc cgtccctggg
     1501 acgcgagata ctcctcgacg cgaagcggat cggcctctcc gacgcgcgga tcgcccggga
     1561 gacgggatcg accgagcgag ccatccgtga ccggcgcgag caggtgggtg tggtcccggt
     1621 cttcaagtcc gtggacacgt gcggcggcga gttcccggcc cggacgccct atctctactc
     1681 gacctacgac gaggaatcgg aggtggagcc cgtctcgcgc cctcgggtcg tgatcctcgg
     1741 tgcgggaccg aaccggatcg gccagggcat cgagttcgat tacgcctgcg tgcacgccgc
     1801 gttcgccttg gaggaggcgg gcttcgaatc ggtgatggtc aactccaatc cggagaccgt
     1861 gtctacggac tacgacacgt cgagccggct gtacttcgaa ccgctctcgg ccgaagacgt
     1921 gctggccgtc tgccgagccg agcgaccggc gggggtgatc gcgcagctcg ggggccagac
     1981 acccttggag ctcgcgagga ccctccagga cgcgggcttc ccgatcctcg ggacctcgcc
     2041 ggacgcgatc gatctcgccg aggatcgggg gcggttcgcg gagctcctcc acgagatgga
     2101 catcccggcg ccaccgcacg gcgaggcacg gacgatcgac gaggctcgca tcgtggcccg
     2161 ccggatcggc tatcccgtgg tcgtccgacc ctcgtatgtc ctcggcggtc gcgcgatgga
     2221 gatcgtctac gacgaccacg agctcgagac gttcgtccgt acggcagccg aggccagccg
     2281 tgagcacccg gtgctggtcg atcggttcct cgagggcgcg atcgaggtcg acgtcgacgc
     2341 cgtctgcgat ggggtggacg tcttcatcgg cgccgtgatg gagcacatcg aggaggcggg
     2401 cgtccattcg ggcgactcct cgtgccagat cccgccggcc acactctccg acgaggagct
     2461 cgatcggatc gaggacatca cccggcgtct c
//
LOCUS       DASUVE010000242         7326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_113215, whole genome shotgun sequence.
ACCESSION   DASUVE010000242 DASUVE010000000
VERSION     DASUVE010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113215"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VF036_05270"
     CDS             complement(<1..599)
                     /locus_tag="VF036_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264674.1"
                     /translation="MQSRVSHRPSVRGMVGLVVVLLAAACAPPRYEYVRNTKARTAFR
                     IPTEWTVFEESTVSGVDSAADTPDTIDWLVGIDANPKPNVANVYPSAFATDFPQGFAA
                     VLTLTGESRDVASLQVLRNLLIPIDQIRDDLGSDAVRVLEYDDALVRDGYRGLQMVVQ
                     VQEAALLGDGQPASGSGGAGFLSDTYVQMNQVMLVDDVT"
     gene            complement(673..1116)
                     /locus_tag="VF036_05275"
     CDS             complement(673..1116)
                     /locus_tag="VF036_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX6264675.1"
                     /translation="MPDERDLAILTALQAEGRATYADVGATVGLSASAVHERVRKLEH
                     QGVIRGYRAVVDPESVGLYVTALIAAIPLDPHQPDDLPARVREFPEVEDCYSVAGEAN
                     YVLKVRTKTTGDLEDLIRRLREKAAVTTNTTIALSIPFEGRPLST"
     gene            1237..1956
                     /locus_tag="VF036_05280"
     CDS             1237..1956
                     /locus_tag="VF036_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HEX6264676.1"
                     /translation="MRRRPTRPTGRGDTPGPRSIPISEPGGGTRVLTVLGSADDAAYR
                     ASVGPLVGRIERSLGPEVVAIRAKRDDAGGWTLGPWRAARAAWRARIRRAILEAPEAT
                     FAVADVRECYASISPETITALLGPEGAQAVALLHRFHERGVRGLPVGPEPSAVLANAA
                     LSRMDRAIRNAGVRHLRWVDDVILWGSAEEVRTAMVAMRTAGEAIGLELHRGKTRIFE
                     DLEEATGVLLGERDSSIIAAP"
     gene            1953..3452
                     /locus_tag="VF036_05285"
     CDS             1953..3452
                     /locus_tag="VF036_05285"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6264677.1"
                     /translation="MRTLYRASRVVTLSHPTFGEWILVDDRHVQRVGTGEPPEAELTI
                     DLPGTTIVPGFIDSHVHLTATGRSVANADVRESRSKQDFLALVRGRATEGVAVVHVEG
                     LDETRWDMHELPTAHDFDAITPTPVVATRADGHISVVSSSTLAAIDLADADGVEVDAE
                     RHPTGRLTKEANRRAVAWVEASYDTRKIEGFQLQAAALAASRGVTAVHEMSLEAADLE
                     VLLGHRSRLPVDVAPILASTSLPEAMERRLAAIGGDLPVDGSIGARTAALSHAYDDGA
                     DTGELYHDDDAMAEFFHAAHNAGLQVGVHAIGDRAIEQVLATWERVYLALDSRERRHF
                     RARRHRIEHFVLPSADQIERTAMLGLAVSVQPAFDRLWGQGGGMYEERLGPARAMAAH
                     PIRTMLERGIEVGVGSDAPVAPLDPMLAIAALEEHHEPTQRLTRLEGLKLHRVGSARL
                     GHQDGKKGALEPGWHADFAAYDADPLDSSTVEGLRPVLTVSLGREVFRA"
     gene            3765..4121
                     /locus_tag="VF036_05290"
     CDS             3765..4121
                     /locus_tag="VF036_05290"
                     /inference="COORDINATES: protein motif:HMM:NF025204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HEX6264678.1"
                     /translation="MAERTEEREDDDVGTDRELDELEPTEEDLNEAATPAAADVESIH
                     EVLEKQEARDDEDEADEEEPVVPISTTKADDRLEPIEARVVPIQATEFVCKNCYLVKH
                     RSQLADKRRMFCRDCA"
     gene            4220..5500
                     /gene="lnt"
                     /locus_tag="VF036_05295"
                     /pseudo
     CDS             4220..5500
                     /gene="lnt"
                     /locus_tag="VF036_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
     gene            5573..6370
                     /locus_tag="VF036_05300"
     CDS             5573..6370
                     /locus_tag="VF036_05300"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX6264679.1"
                     /translation="MVPRRRRRERPDPEPLAEGVRSLVILPTYDEAATVRDVLAGVLE
                     HPGVDALVVDDSSPDGTARIVRELMAGEPRLRLIERPTRSGLASAYQEGFRRALEERY
                     DLIVEMDSDLSHDPVQLGSLLAGARDRFHLVVGSRYVPGGSVTNWSRFRLALSRVGNT
                     YARLMLGVPVHDATSGYRVYRRELLSDLVAQPFHADGYGFQVELVMRSWLRGWAIGEV
                     PITFREREHGHSKLSRRIVVEALWLVTRWGIALRATRRPYRGGPAAA"
     gene            6687..>7326
                     /locus_tag="VF036_05305"
     CDS             6687..>7326
                     /locus_tag="VF036_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264680.1"
                     /translation="MDGRKHRRRGLLGPIVALALLVGQAGILTARAAPPLDSPGPPSA
                     DGVVPVIADTQSSNDDCGQLGFDHGVSIASNGQASSGAMTVTVSGYNGPTGFADWSAT
                     LPVHGVYVKGGPSGGDLFGYPAGDTGDHDLHTPQKPDGGYYSVSHLAICWNDVAPEPD
                     VSVVKVNDPGGVVQSGDSITYTLSVSNHGTGTAVDVHVADQLPVGVTFADATA"
BASE COUNT         1074 a         2605 c         2539 g         1108 t
ORIGIN      
        1 tgagtcacgt cgtcgaccag catcacctgg ttcatctgca cgtacgtatc ggagaggaat
       61 ccagcgccgc cggatccgga ggcgggctgc ccgtcgccga gcagcgcggc ctcctgcacc
      121 tggaccacca tctggagccc ccggtacccg tcccgtacca gggcgtcgtc gtattccagc
      181 acccggaccg cgtccgagcc caggtcgtcg cggatctgat cgatcgggat caggaggttc
      241 cgcagcacct gaaggctcgc cacgtcccgg gattccccgg tgagcgtgag gaccgccgcg
      301 aagccctgag ggaagtcggt cgcgaaggcc gacgggtaga cgttcgcgac gttcggcttc
      361 gggttggcgt cgatcccgac cagccagtcg atcgtgtccg gggtgtcggc ggccgagtcg
      421 acccccgaca ccgtggactc ctcgaacacg gtccactccg tggggatccg gaaggccgtc
      481 ctggccttcg tgttgcggac gtactcgtac cgaggcgggg cacaggcagc ggccagcagc
      541 accacgacga gtccgaccat gcctctgacc gaggggcgat gggaaaccct cgactgcatc
      601 tcatcatgat gccacgactc gcacgaggaa ccagaccgat cacgtatcgg cagcccgggg
      661 acggaacctg tatcaggtgc tcaacgggcg gccttcgaac gggatcgaaa gcgcgatcgt
      721 ggtgttcgtg gtcaccgcgg ctttctcgcg gagccgacgg atcagatcct ccaggtcacc
      781 cgtggtcttc gtccggacct tcagcacgta gttggcctcg cctgcgaccg agtagcagtc
      841 ctcgacctcc gggaactccc gcaccctcgc cggcaggtcg tcgggctgat gcggatcgag
      901 cgggatcgcg gcgatcagcg ccgtcacgta cagaccgacg gactccggat ccacgacggc
      961 ccggtatccg cggatcaccc cttggtgctc gagcttccgg acgcgctcgt gcacggcgct
     1021 cgccgagagc cccaccgtcg cgccgacgtc cgcgtacgtg gcccgaccct ccgcctgcag
     1081 ggccgtgagg atcgcgagat cccgctcatc cggcacggcg gcatggtccc acacggggga
     1141 ccgagaaatc cccgcgacgg ccgattgctc ccactcacgc tccggcacta cggtcggcgt
     1201 ctcctcctcc cgatctcccc ccgaggagcg gaagcgatgc gccggcggcc cacgcggccg
     1261 acgggcaggg gagacacccc cggcccgcga tcgatcccca tctccgaacc gggcggtgga
     1321 acccgcgtgc tgaccgtgct cggctccgcg gacgatgcag cgtatcgagc gtcggtcggc
     1381 ccgctcgtgg gccggatcga gcgttccctg gggcccgagg tcgtggccat ccgcgctaag
     1441 cgagacgatg ctggcggatg gacgctcggg ccctggagag cggcacgtgc cgcgtggcgc
     1501 gccaggatcc ggcgggcgat cctcgaggct ccggaggcga cgttcgcggt cgccgacgtg
     1561 cgcgaatgct acgcctcgat ctcgccggag acgatcaccg cgctcctggg tccggagggc
     1621 gcgcaggcgg ttgccctcct ccaccggttc cacgagcgag gcgtgcgcgg gctccccgtc
     1681 ggcccggagc cgtccgccgt cctggcgaac gcggccttgt ctcggatgga tcgcgcgatc
     1741 cggaacgccg gcgtccggca cctccggtgg gtcgatgacg tcatcctgtg ggggtccgcc
     1801 gaggaggtcc ggaccgcgat ggtcgcgatg cgcacggccg gggaggcgat cggcctcgag
     1861 ctccatcggg gcaagacgag gatcttcgag gatctcgaag aggcgaccgg ggtgctgctc
     1921 ggtgagcggg actcctctat catcgcggcg ccgtgaggac cctctaccgc gcgtcgcggg
     1981 tcgtgacgct ttcgcatccc acgttcgggg aatggatcct cgtggacgat cgccacgtcc
     2041 agcgcgtcgg caccggagag ccccccgagg ccgagctcac gatcgatctc cccgggacga
     2101 cgatcgtccc cggcttcatc gacagccacg tccacctgac cgcgacgggt cgttccgtcg
     2161 cgaacgccga cgtgcgggag tcccgctcca agcaggactt cctcgccctg gtgcgcgggc
     2221 gcgccaccga gggggtcgcc gtcgtgcacg tcgaggggct cgacgagacc cggtgggaca
     2281 tgcacgagct cccgaccgca cacgacttcg acgcgatcac gccgacgccc gtcgtggcga
     2341 cgagggcgga cgggcacatc tccgtcgtga gctcgtcgac cctcgcggcg atcgacctcg
     2401 ccgatgcaga cggcgtcgag gtcgacgccg agcgacatcc gaccggccgc ctcacgaagg
     2461 aggccaaccg acgggccgtc gcgtgggtgg aggcctcgta cgacacgagg aagatcgaag
     2521 ggttccaact gcaagccgcc gcgctcgccg catcacgggg cgtgacggcg gtgcacgaga
     2581 tgtccctcga ggcagcggac ctcgaggtgc tcctcggtca tcggtcccgg ctgccggtcg
     2641 acgtcgcacc gatcctcgcg tcgacgtcgc tccccgaggc catggaacgg cggctcgccg
     2701 cgatcggcgg ggacctcccg gtcgacggtt cgatcggcgc ccggaccgcg gcgctatccc
     2761 acgcctacga cgacggcgcg gacacgggcg agctgtacca cgacgacgac gcgatggcgg
     2821 agttcttcca cgcggcccac aacgcgggac tccaggtggg cgtgcacgcg atcggagacc
     2881 gcgcgatcga acaggtcctc gccacgtggg agcgtgtcta cctggcgctg gattcccgtg
     2941 aacgacggca cttccgcgcc cgtcgccacc ggatcgagca cttcgtcctc ccctcggcgg
     3001 atcagatcga gcggaccgcg atgctgggtc tcgccgtgtc cgtccaaccg gcgttcgatc
     3061 ggctctgggg ccagggaggc gggatgtacg aggagcgcct gggccccgcc cgggcgatgg
     3121 ccgcacaccc gatccggacg atgctcgagc gagggatcga ggtcggcgtc ggctccgacg
     3181 cacccgtcgc acccctcgac ccgatgctgg cgatcgccgc gctcgaggaa caccacgagc
     3241 cgacccagcg gctgacgcgg ctcgaggggc tcaagctcca ccgggtgggg agcgcccgcc
     3301 tcgggcacca ggacggcaag aagggggccc tcgagcccgg gtggcacgcg gacttcgccg
     3361 cgtacgacgc cgacccgctc gactcgtcca ccgtggaggg gctccgtccg gtcctgacgg
     3421 tctccctggg tcgcgaagtc ttccgcgcct gatcgctggg tggtcttgtc actgtttctc
     3481 ccggcgccgg gttgctctcc cgctcggccg tcggtaacgt cctgcctgcg tcgccgcccc
     3541 ggcggagacg cgcagcggag cccgagaggc tgagtagaga acgcggcgcc ccggcgtcgc
     3601 ggccccaaag gtctctcggg cttccgcgtt tccaggctcc gcactccggg aacatcctga
     3661 gcctgcgagg cgtccttccc ctgcgacgga gtcgcacgag gcacgcgggg gagcgagcgc
     3721 tagaatccgc ggccggatcg aggacccgaa cccggaggaa ccaggtggcc gagcgcacgg
     3781 aagagcgtga agacgacgac gtcggaacgg accgggagct cgacgagctc gagccgacgg
     3841 aggaggacct gaacgaagcc gccacaccgg cggctgccga cgtcgaatcg atccacgagg
     3901 tcctcgagaa gcaggaggcc cgtgacgacg aggacgaggc cgacgaggag gagcccgtcg
     3961 tcccgatctc gacgacgaag gcggacgatc gtctcgagcc gatcgaggct cgggtcgttc
     4021 ccatccaggc gaccgagttc gtatgcaaga actgctacct ggtgaaacat cgcagccagc
     4081 tcgcggacaa acggcggatg ttctgccgcg actgcgcctg atcgatccca ggctgccggt
     4141 cgcgctcacc ctctccctcg tcgggggcgt gctgtccgcg ctcgcgtttc cgcccgtggc
     4201 cgcctgaccg ctcgctttcg tcgcccccgc gttgctcctg atcgggctcc ggaacgccac
     4261 accggcacgc ggcttcgccc tcgggctcgt ctacggactc gtcggcttcg gactcacgct
     4321 ctcgtggctc cagctcttcg gcaccctcgc ctggggcgcc ttgacggtcc tcacggcggc
     4381 gtccgtggcg ttgttcggcg cctgctcccc gttggtgcgt cggtcgggcc acccgatcct
     4441 gaatgcgctc ggggctgcgg cgttgtggac gtcgatcgac tggctccggg gactctggcc
     4501 gctcggtggc ttcacgtggg gctccctcgg cgcctcgcag gtggccaacc cggtcacggt
     4561 tcgcctcgcc gtcctcgccg gggtgttcgg ggtcacgttc gcggtggcgt tcgcggccgc
     4621 cctcgtcgtc gaggccgccg gcgcggagag gacgccgacg cggctcgccg ccatcggcct
     4681 cgcggcgatc gcgacgctgg gaccggcggc gatccccttc gcggaggcca cggggcgcca
     4741 gatcgacgtg gccgcgatcc aggtcgactt cctcgaaggg gtcgcaggac ggacgcggga
     4801 ggaaggagac gtcgcggtca cgaggctcaa cctcgagctc catgcgacgc tgagtggcga
     4861 tcctcccgat ctggccgtct ggggagagag cgctctcgac ccgggctcgc tctcgatcct
     4921 cgacgaggtg cggagcacga tcgcggaggt cgggacgccg gtcctcagcg gggccacctc
     4981 ggcggatatc cgatcgggtg aggaaggcga cgccctgttc aaccaggcgg tcgtcttcga
     5041 cggcggggga gcggtggtcg acgtgtacag gaagacccac ctcgtcccgt acggcgagta
     5101 catcccctgg aagccagtcg tcgggtggat ctcggccctc gaacagatcg cctacgagct
     5161 cacgccgggg gagcgactcc acacgttgac ggcgccgggg ctccctcggt tcggagcgcc
     5221 gatctgcttc gagaacagct tcccggcgct cggtcgcgag ctcgtccgcc agggcgcgga
     5281 gttcctcgtg gtcctcacga acaacgcgtc ctacgaccgc acggccgcat ccgcccagca
     5341 tctgcagatg tcgcgcctcc gtgcggtcga ggacggcagg tggatcgtcc acgcggccgt
     5401 ctcggggatc accgcgttca tcgacccggc gggccgcgtc cacgacgaga ccgagctgtt
     5461 cgagccgggg ctgatacgga agacgatcag ggcctcgtcg gagcggacgt ggtcggtgcg
     5521 actgggggac tggctgccca tcctctcgtt gatgatcgtg gtgggtctcg gcatggtacc
     5581 ccggcgtcgg cgtcgcgagc ggcccgaccc cgagccgctc gccgagggcg ttcgttcgct
     5641 ggtgatcctg cccacctacg acgaggcggc gaccgtccgt gacgtcctgg ccggcgtcct
     5701 cgaacacccg ggggtggacg cgctggtcgt cgacgactcc tcaccagacg gcaccgcacg
     5761 gatcgtccgg gagctgatgg ccggggagcc ccggctccga ctcatcgaac gacccacgag
     5821 gtccgggctc gcgagcgcct accaggaggg cttccgcagg gcgctcgagg agcggtacga
     5881 cctgatcgtg gagatggact ccgacctctc ccacgatccc gtgcagctcg gttccctcct
     5941 ggccggcgct cgcgaccgct tccacctcgt ggtggggagc cggtacgtcc ccggaggctc
     6001 cgtgaccaac tggagccggt tccgcctggc gctctcgagg gtgggcaaca cgtatgcgcg
     6061 gctgatgctc ggcgtgccgg tgcacgatgc cacgagcggc taccgcgtct accgtcgcga
     6121 gctgctctcg gacctcgtcg cccagccgtt ccacgccgac ggttatggct tccaggtcga
     6181 gctggtgatg cgctcctggc tccggggctg ggcgatcggg gaggtgccga tcacgttccg
     6241 cgaacgggaa cacggtcatt cgaagctctc gcggcggatc gtcgtcgagg cactctggct
     6301 cgtcacccgc tggggcatcg ccctcagggc gaccaggagg ccgtaccgag gagggccggc
     6361 cgccgcctga ggatcctctg ccctcgcgct gacgctaccc cgataacgtt cattatgtaa
     6421 cttccctggg aacgaccgtt gagggctaac tccctcccca gccgatctgg cacccgagcg
     6481 atccgcccga cacatcgagg gctgacctcg ttcttgcttc ggccccaggt ggatgtgaag
     6541 ctccgtccgg catcgatccc gccgatcggt cggatggtct gcgccttccg gccgatgccc
     6601 acgtcccccg tcccccgtag ggtgtgggtc gaccggccga cagcgcgttc tgtgacccga
     6661 cgtggcgtga aagaccagga ggagagatgg acggacggaa gcaccggcgc cggggactcc
     6721 tgggaccgat cgtggcgttg gcgctcctcg tcggtcaagc ggggatcctg acggcgcgtg
     6781 cggcaccgcc cctggactcc cccgggccgc cttccgccga cggcgtcgtc cctgtcatcg
     6841 ccgacaccca gtcctcgaac gacgactgcg gccagctcgg cttcgaccac ggggtctcga
     6901 tcgcgagcaa cggccaggcg tccagcggcg ccatgacggt caccgtctcg ggctacaacg
     6961 gtccgacagg gttcgcggac tggtctgcga cccttccggt ccacggcgtc tacgtgaagg
     7021 gcggaccgag cggcggcgac ctcttcggct atccggccgg cgataccggc gatcacgacc
     7081 tccacacccc ccagaagccg gatggcgggt actactcggt gagccacctc gccatctgct
     7141 ggaacgacgt ggcgcccgaa cccgacgtgt cggtcgtgaa ggtgaacgat ccgggcggcg
     7201 tcgtccagag cggcgactcg atcacctaca ccctgtcggt gtccaaccac ggcaccggca
     7261 ccgcggtcga cgtgcacgtc gccgatcaac tgccagtggg ggtgacgttc gccgacgcca
     7321 ccgccg
//
LOCUS       DASUVE010000243         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_113483, whole genome shotgun sequence.
ACCESSION   DASUVE010000243 DASUVE010000000
VERSION     DASUVE010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113483"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2185
                     /locus_tag="VF036_05310"
     CDS             <1..>2185
                     /locus_tag="VF036_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX6264681.1"
                     /translation="VTSRRLPEGGSRIDRSKPVTFRFDGTEYEGFEGDTLASALLANG
                     VTGGFRSPILRRPRGIMTAGPEEPNAFVEVSEPWFDPIVAATMVELVDGLVAEPRAGI
                     GRLTAANTPRQRAEHRHAHVETLVVGGGPSGRAAAADAASGGERVMLVDERHRIDDPP
                     NDVTVLARTTALGVYDDGYVVAYERLPDRDRLWHVRAGRVILATGATERPIAFAGNDR
                     PGVMLTSAAATYVERFGVVLGDRVTVVTTNGEAVDSLEPLRAAGIDVQAVRTDAPPEA
                     DEHTSLLVSGGWNPNLGLWRGIGGSVRYDDERACFVPGQGPPWLETTGTARGDGLPAV
                     DPVWLVHGDRSASFVDLQRDQTVADIADALDAGLTSVEHVKRATYIGTAIDQGRTSGV
                     LAAAIVNQLLGAGPDAQGPTSARPPAVPVPYATITGPDRGDLLDPIRRTRIHPWHEAH
                     GAVFEDVGQWKRPRYFPRDAEGMDAAVARECLAVRNAAGVLDASTLGKIEVVGPDAAA
                     FLDRMYTNRMSTLAVGSIRYGFLLGLDGMVADDGVAMRLAEDRFFVTTTTGNAAMVLD
                     RFEEWLQTEWPELRVYCTSVTEQWADFAIAGPKAAEVLTALGTDIDLSAEAFPFMSFR
                     DGVVAGIPSRAARVSFTGELSYEILVDGRRALELWEAVLGAGDPIGITPYGTEAMHVL
                     RAEKGFVIVGQDTDGTVTPDDLGMGWIVRKDGSDFIGRRSLRRADT"
BASE COUNT          317 a          744 c          805 g          319 t
ORIGIN      
        1 gcgtgacgtc gcggcggctc cctgaaggag gctcgaggat cgaccggtcg aagccggtca
       61 cgttccgctt cgacggcact gagtacgagg ggttcgaagg cgacacgctg gcgagcgcgt
      121 tgttggccaa cggggtgacc ggtgggttcc ggtctccgat cctccggcgg cctcgcggca
      181 tcatgaccgc cgggccggag gaaccgaacg cgttcgtcga ggtcagcgag ccgtggttcg
      241 acccgatcgt cgcggcgacg atggtcgagc tcgtggacgg gttggtggcc gagccgcggg
      301 cggggatcgg tcgtcttacc gcggcgaaca ccccgaggca gcgcgcggaa caccggcacg
      361 cccacgtcga gacgctcgtc gtcgggggcg gtccgtccgg ccgtgcggct gcggccgacg
      421 ctgccagcgg cggtgagcgg gtgatgctcg tcgacgagcg acatcggatc gacgatccgc
      481 cgaacgacgt gaccgtcctc gcgcgcacga cagccctcgg tgtgtacgac gacggctacg
      541 tggttgcgta cgagcggctc cccgaccgcg accggctctg gcacgtgcgc gcgggccgcg
      601 tgatcctcgc gaccggcgcg accgagcgcc cgatcgcgtt cgcggggaac gaccgcccgg
      661 gcgtgatgct cacctccgcc gccgcgacct atgtggagcg gttcggcgtc gtcctcgggg
      721 atcgcgtgac ggtggtcacg accaacggag aagcggtcga ctccctcgaa ccactccgcg
      781 ccgccggaat cgacgtgcag gccgttcgga ccgatgcacc gcccgaagcg gacgagcaca
      841 ccagcctcct cgtctcggga ggctggaacc cgaacctcgg gctctggcga gggatcggcg
      901 gatcggtccg gtacgacgac gagcgcgcct gcttcgtccc cggtcagggg cctccctggc
      961 tcgagacgac ggggacggca cgcggcgacg ggctgccggc ggtcgatccc gtttggctcg
     1021 tccacggtga tcggtcggca tcgttcgtcg atctgcagcg ggaccagacc gtcgccgaca
     1081 tcgccgacgc cctcgacgct gggctcacat ccgtggagca cgtgaagcgc gcgacgtaca
     1141 tcgggacggc gatcgatcaa ggtcgcacga gtggcgtact cgctgccgcg atcgtgaatc
     1201 agctgctggg cgccggcccg gacgcgcagg ggccgaccag cgctcgtccg ccggccgtgc
     1261 cggtgcccta cgcgacgatc acggggccgg atcgcggcga cctgctcgac ccgatccgcc
     1321 ggacgcgcat ccatccgtgg cacgaggcgc acggggccgt cttcgaggac gtgggccaat
     1381 ggaagcgacc ccggtacttc ccgcgagatg cagagggcat ggacgccgcg gtcgcccgcg
     1441 aatgcctcgc ggtgcggaac gcggccggcg tgctcgatgc ctccaccctc gggaagatcg
     1501 aggtggtcgg ccccgacgcc gccgcgttcc tcgatcggat gtacacgaac cggatgagca
     1561 cgctcgccgt cggctcgatc cgctacgggt tcctgctcgg cctcgacggc atggttgccg
     1621 acgacggggt ggcgatgcgc ctcgcggagg accgcttctt cgtcacgacg acgaccggga
     1681 acgccgcgat ggtcctggat cgcttcgagg agtggctcca gaccgagtgg ccggagctgc
     1741 gcgtctactg cacgagtgtc acggagcagt gggccgactt cgcgatcgcg ggaccgaagg
     1801 cggctgaggt tctcaccgcc ttggggaccg acatcgacct gtcggccgag gccttcccct
     1861 tcatgtcgtt ccgggatggc gtggtcgcgg ggatcccctc gagggcggcc cgggtgagct
     1921 tcacgggaga gctctcctac gagatcctcg tcgacggccg ccgcgcgctc gaactgtggg
     1981 aggccgtgct cggcgccggg gatccgatcg gtatcacgcc gtacggcacc gaggcgatgc
     2041 acgtcctccg ggccgagaag gggttcgtga tcgtcggaca ggacaccgac ggcaccgtca
     2101 cgcccgacga cctggggatg ggatggatcg tccgcaagga cggctcggac ttcatcgggc
     2161 ggcgctcgct gcggcgcgca gacac
//
LOCUS       DASUVE010000244         6365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1057112, whole genome shotgun sequence.
ACCESSION   DASUVE010000244 DASUVE010000000
VERSION     DASUVE010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057112"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..145
                     /locus_tag="VF036_05315"
                     /pseudo
     CDS             1..145
                     /locus_tag="VF036_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009406268.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
     gene            complement(106..1341)
                     /gene="glmS"
                     /locus_tag="VF036_05320"
     CDS             complement(106..1341)
                     /gene="glmS"
                     /locus_tag="VF036_05320"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX6264682.1"
                     /translation="MCGIVGYVGPDEALPIVLEGLRRLEYRGYDSAGVAVLDGDLTVV
                     KRAGKLSELVTALDEDGHPAGIVAMGHTRWATHGAPTHRNAHPHLDCSETIAVIHNGI
                     IENFQQLRARLEKEGHTLTSETDTELVAHLIEERLRDGGSLVEAVRATVRELEGAYSL
                     VVMSSEEPGLLVGVTVASPLVVGVGSGESILASDIPAVLGRTTTVIPVDEGRIVEVRA
                     DGATFTDFDGSVVHPESIEVDWDSARAEKGGFDTFMLKEIHEQPSAIRDTLVGRVDEM
                     RRLALDDLRISDDVLREVNKVFVVACGTAYHSGLVAKYAIEHWTRLPVEIDIASEFRY
                     RDPVLGSDTLTLAVSQSGETIDTLEAARHARRQGSPLLAVTNTVGSSLAREADGVIYM
                     HAGPEIGVAATPISGPACM"
     gene            complement(1408..2739)
                     /gene="glmM"
                     /locus_tag="VF036_05325"
     CDS             complement(1408..2739)
                     /gene="glmM"
                     /locus_tag="VF036_05325"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX6264683.1"
                     /translation="MARLFGTDGVRGIAGSELTATLARGLGRAAVRVLADGVEHRPVF
                     VLGRDPRESGTWLEEALVGGIHEAGGDVLLAGVEPTPAIAFLTIDVGADAGVVISASH
                     NPPEYNGIKFFGPNGMKLPDVVEDRIEAGLSDTSSPAAHEGSIQDLAGGRERYLAHLE
                     AAAGARLNGMTIVVDCANGAASGLAPTLYERLGATVHPIHDAPDGRNINVGCGALHPE
                     VVGAAVVELGADAGVSHDGDADRALFVDATGTPIDGDQILAACAIAMKEAGALPSDAV
                     VTTVMANLGFHRAMRDAGITVSATKVGDRYVLEEMLEEGVMLGGEQSGHLIFRDHATT
                     GDGLLTAVRFLSLAVGSGRTVADLAGVMQRYPQVMENVEVRSLTELDGSDRVWEAVRA
                     AEEELADHGRVIVRASGTEPLVRVMVEAETEDVARRHADAIADEVRKHLGA"
     gene            2936..3268
                     /locus_tag="VF036_05330"
     CDS             2936..3268
                     /locus_tag="VF036_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016864789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6264684.1"
                     /translation="MKLIQDLDTCCTPLLSSPLSEDAAETLATALQALADPARLRLLS
                     LIAACPDSEACGCDLTAPIGLSQPTVSHHLKVLHEAGLLERERRGKWVYFRVLPEPLA
                     SLRSALSA"
     gene            complement(3211..3669)
                     /locus_tag="VF036_05335"
     CDS             complement(3211..3669)
                     /locus_tag="VF036_05335"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX6264685.1"
                     /translation="MKEPLPGVLFVCVHNAGRSQMAAALLERHAAGRIRVRSAGSEPA
                     DRLHPSVVEVMAEVGIDLARERPKLLRDEAVRDSDVVITMGCGDACPVFPGVRYEDWD
                     LEDPSRKPLEDVRRIRDDIDRRVRRLLEGLTAQAESAERSDASGSGSTRK"
     gene            3772..4044
                     /locus_tag="VF036_05340"
     CDS             3772..4044
                     /locus_tag="VF036_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX6264686.1"
                     /translation="MQLIRVFESLDPIRGMLVRGLLEAEGLEVLAKGEGLGPYRTGPV
                     ILFVTEDASDRARELIAAAEDGSLALAPDEQSSGSAEGLAVERSAD"
     gene            complement(4041..4436)
                     /gene="rpsI"
                     /locus_tag="VF036_05345"
     CDS             complement(4041..4436)
                     /gene="rpsI"
                     /locus_tag="VF036_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX6264687.1"
                     /translation="MATTPTTLTTGRRKAAIARVRLVPGTGEFRINDRSLDEYFPTRV
                     HRMVAQSPLRSVGRDKDLDVVATITGGGVNGQAGALRLGIARALLELEPDLRAQLKAE
                     GFLRRDAREKERRKYGLKKARKAPQYSKR"
     gene            complement(4799..5209)
                     /gene="rplM"
                     /locus_tag="VF036_05350"
                     /pseudo
     CDS             complement(4799..5209)
                     /gene="rplM"
                     /locus_tag="VF036_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130859.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
     gene            complement(5299..6045)
                     /gene="truA"
                     /locus_tag="VF036_05355"
     CDS             complement(5299..6045)
                     /gene="truA"
                     /locus_tag="VF036_05355"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX6264688.1"
                     /translation="MSVTVLTLAYDGTAFRGWAMQRDPSIRTIEGELVRHLERVLREP
                     VKLSVAGRTDAGVHARGQVASFRTETSVAPGRLQKALNSALAPEVVVVRARLASDGFD
                     ARFSATARGYVYRIDEAPLADPFTGRFAWHRPGRLALAPMRRAARDLEGRHDFRSFCR
                     HPGGDRSTMRHLRRLTVVRAGDEIAVRAVADAFLHQMVRSLVGTLVAVGAGKQDATAM
                     PSVLAARDRSAAPGGLAPPRGLTLERVSYR"
     gene            complement(6115..>6365)
                     /gene="rplQ"
                     /locus_tag="VF036_05360"
     CDS             complement(6115..>6365)
                     /gene="rplQ"
                     /locus_tag="VF036_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX6264689.1"
                     /translation="KAKQLRPFAEKLITFAKRGDLAARREVLRDIPDRDVVAKLFHEI
                     GPRFEDRHGGYTRILRLGPRKGDGAPMAMIELVEREEL"
BASE COUNT          909 a         2257 c         2163 g         1036 t
ORIGIN      
        1 cgacacgctc gaggccgctc gacacgcacg gcgccagggg tcgcccctcc ttgccgtcac
       61 gaacacggtc ggctcgtcgc tcgccaggga ggccgacggc gtgatctaca tgcacgccgg
      121 gccggagatc ggcgtcgcgg cgacaccgat ctccgggccc gcatgcatgt agatcacgcc
      181 gtcggcctcc cgggcgagcg acgagccgac ggtgttcgtg acggccagga ggggcgatcc
      241 ctggcgccgc gcatgccggg cggcctcgag cgtgtcgatc gtctcccccg actgtgagac
      301 cgcgagggtg agcgtgtcgg agccgaggac cgggtcccgg taccggaact cgctggcgat
      361 gtcgatctcc acgggcagcc gcgtccagtg ctcgatcgcg tacttcgcga cgaggcccga
      421 gtggtacgcg gtcccacacg ccaccacgaa gaccttgttc acctcacgga ggacgtcgtc
      481 ggagatcctc aggtcgtcga gcgcgaggcg gcgcatctcg tcgacccgcc ccaccagcgt
      541 gtcgcggatc gcgctcggtt gttcgtggat ctccttgagc atgaacgtgt cgaagccgcc
      601 cttctccgcc cgagcggagt cccagtcgac ctcgatggac tccggatgca cgacgctgcc
      661 gtcgaagtcc gtgaacgtcg cgccgtctgc tcgcacctcg acgatccgtc cctcatccac
      721 ggggatcacc gtggtcgtcc gtccgagcac cgccgggatg tccgaggcga ggatgctctc
      781 gccggaaccg accccgacga cgagtgggga cgcgaccgtc acgccgacga ggagaccggg
      841 ctcctccgac gacatgacga cgagcgagta cgccccttcc agctcgcgga ccgtcgcgcg
      901 gacggcctcc acgagcgatc cgccgtcccg gaggcgttcc tcgatgagat gggccacgag
      961 ctccgtgtcg gtctccgagg tgagggtgtg cccttccttc tcgagccgtg ctcggagctg
     1021 ctggaagttc tcgatgatcc cgttgtggat cacggcgatc gtctcggagc agtcgaggtg
     1081 cggatgggcg ttgcggtggg tcggcgctcc atgcgtcgcc cagcgcgtgt gacccatcgc
     1141 gacgatcccg gccggatgtc cgtcttcgtc gagcgccgtg acgagctccg agagcttgcc
     1201 cgcccgcttg acgacggtga ggtcgccgtc gagcacggcg acgcccgccg agtcgtaccc
     1261 gcggtactcc agacgtcgaa gtccctcgag gacgatcggg agggcctcgt cgggccccac
     1321 gtagccgacg atgccgcaca tggagcatcg agggtagcag ggcggtccgt ccgcgagaac
     1381 cggacgaggg cctccgagag acggcgctca ggcgccgagg tgcttcctca cctcgtctgc
     1441 gatcgcatcc gcgtggcggc gcgcgacgtc ctccgtctct gcctcgacca tcacccggac
     1501 gagcggttcg gtgccagacg cgcggacgat cacgcggcca tggtcggcga gctcctcctc
     1561 cgccgctcgg accgcctccc acacgcgatc ggagccgtcg agctcggtga ggctccgcac
     1621 ctcgacgttc tccataacct gcgggtaccg ctgcatcacg ccggcgagat cggcgaccgt
     1681 gcgaccgctg cccaccgcga gggagaggaa ccggaccgcg gtcaggagac cgtcgccggt
     1741 cgtcgcgtgg tcacggaaga tcaggtggcc ggactgctcc cctcccagca tcacgccttc
     1801 ctccagcatc tcctcgagga cgtaccggtc gcccaccttc gtggcggaga ccgtgatccc
     1861 ggcgtcccgc atcgctcgat ggaagccgag gttggccatc acggtcgtga ccacggcatc
     1921 ggagggaagg gcaccggctt ccttcatcgc gatcgcgcac gcggcgagga tctggtctcc
     1981 gtcgatcggc gttcccgttg cgtcgacgaa cagcgcgcgg tcggcgtctc cgtcatggga
     2041 gacgccggcg tccgccccca gctccacgac cgccgccccc acgacctccg ggtggagggc
     2101 cccacagccg acgttgatgt tgcgcccgtc gggagcgtcg tggatcgggt gcaccgtcgc
     2161 cccgagccgc tcgtagagtg tcggggccaa gccggacgcc gcgccgttcg cgcaatcgac
     2221 gacgatcgtc atcccgttga gccgtgctcc cgccgcggcc tcgaggtgtg ccaggtatcg
     2281 ctcgcggcca ccggcgaggt cctggatcga cccctcgtgg gcggccggcg acgaggtgtc
     2341 cgacagcccg gcctcgatcc ggtcctcgac gacatccggg agcttcatgc cgttcgggcc
     2401 gaagaacttg atgccgttgt actcgggagg gttgtgtgac gccgagatga cgacgccggc
     2461 gtcggcaccc acgtcgatcg tcaggaacgc gatggcagga gtgggctcca cgcctgccaa
     2521 gagcacgtct cccccggcct cgtggatccc cccgacgagg gcttcctcga gccacgtccc
     2581 cgactcccgt ggatcccttc cgagcacgaa cacgggccgg tgctcgacgc cgtcggccaa
     2641 cacccgaacg gccgcccggc cgagccctcg cgcgagcgtc gcggtgagct cggagccggc
     2701 gatccctcgg acaccgtcgg tcccgaagag gcgcgccatc gggcaggtga tgctactgcg
     2761 ggtggcgaaa cgcggggcct ccggccccgc gcgccgccac cctcaggtgc ctagcagcag
     2821 ccgggcgggc agcagtccgg atccatccgg gtcacctccc atcgactctg gccatcacat
     2881 cgatggcggt cgatatgttg ttactacgta tcgacatctg tcaatatgat tattcatgaa
     2941 gctgatccag gacctcgaca cctgctgcac gcctctgctg tcgtcacccc tgagcgagga
     3001 cgcggccgag acgctggcga cggccctcca ggcgctcgcg gacccggcca ggctgcgcct
     3061 cctgagcctg atcgccgcgt gccccgactc ggaggcatgc ggatgcgatc tgacggcgcc
     3121 gatcggcctg tcgcagccga cggtcagtca ccacctgaag gtcttgcacg aggccggtct
     3181 cctcgagcgg gagcgccggg ggaagtgggt ctacttccgg gtgctcccgg agccgctcgc
     3241 gtcgctccgc tcggcgctct ccgcctgagc cgtcagcccc tccagcagac gccggacccg
     3301 tctgtcgatg tcgtcgcgga tccgtcggac atcctcgagc ggcttcctcg aagggtcctc
     3361 gagatcccaa tcctcgtagc ggacaccggg gaagacgggg cacgcgtctc cacaccccat
     3421 cgtgatcacg acgtcggagt cgcgcacggc ctcgtctcgc aagagcttcg gccgctctcg
     3481 ggcaagatcg atcccgacct cggccatgac ctcgacgacg gagggatgca gacggtcggc
     3541 gggctcggag ccggccgatc ggacgcggat acgcccggcg gcatgccgct cgagaagggc
     3601 ggcggccatc tggctcctcc cggcgttgtg gacgcacacg aacaggacgc ccggcagagg
     3661 ctctttcatc cgagccagcg tacgcgctcg cgggtcaccg atggtgaacg gcgcgcgggt
     3721 catcggtgaa cgtccgtgga cgttcacgtc ggtccgggcg atgatggatc ggtgcagctc
     3781 atccgcgtgt tcgagtcgct cgacccgatc cgagggatgc tcgtgcgtgg gctcctcgag
     3841 gccgagggac tcgaggtact cgccaaggga gaggggctcg ggccctatcg gacggggcca
     3901 gtcatcctct tcgtgaccga ggacgcgtcc gatcgagcga gggagctcat cgcggccgcg
     3961 gaggatggat cgctcgcgct ggcacccgat gagcagtcct cgggctcggc ggaggggctc
     4021 gcggtcgagc gctccgccga ctagcgcttg gagtactgcg gagccttccg ggccttcttg
     4081 agcccgtact tacgacgctc cttctcccgg gcgtcgcggc gcaggaatcc ctcggccttg
     4141 agctgtgccc ggaggtccgg ctcgagctcg aggagcgcgc gggcgatgcc gaggcggagc
     4201 gcccccgcct gtccgttgac gccaccgccg gtgatcgtgg cgacgacgtc gaggtccttg
     4261 tcgcggccga cggaccggag cggtgactgc gccaccatcc ggtggacgcg ggttgggaag
     4321 tactcatcga gcgaacgatc gttgatgcgg aactcgcctg ttccgggcac cagccggaca
     4381 cgggcgatcg ccgccttgcg tcgtccggtg gtgagggtcg tcggggtggt cgccactacg
     4441 tctccttgtt cttcgccgtc gtcttcgcgg cggtgggctt cttcgccgtg gcggacttcc
     4501 tcgcggtcgg ctttttcgta gcggtcgact tcctcgccga ggtcttggcg gtggtcgact
     4561 tcgcggtggt cggcttcttc gccgtcgtct tgcgcgccgt ggttctcgtc cccgccgacg
     4621 tggccgcggc cttcggcgac gatgtcgtgg tggccgccgg cttcgtcgcc gccgacgatc
     4681 gtttcgcagg tgtctcggcg gcttcgccct tcggcgtggc cttcttctcg gtcgtgatcg
     4741 aggcgagcga cgtgggcagg ccctcccaga cgggtcgcat ccccatcgtc cactcctggg
     4801 gcttctgtgc ctgatggttg tgctccggcc cgggaaccac gtgaagcttc ttgatcatgg
     4861 cgcgaccgag ccggttcttc gggagcatcc cccggacggc cttctccacg acgaacgccg
     4921 gccgcttctc caggagatcc tcgtacaggg tgccggtgat gccaccggga tgcccggagt
     4981 gccgccaggc gaccttcttc gtctccttgc cgcccgtgag caccaccttc gcggcattga
     5041 tgatgatcag attgtcgccg acgtccatgt gcggagcgaa gatcggcttg tgcttgccgc
     5101 ggaggaccat cgcggcctcc gtggcaaccc ggccgagcac ctgctcgccg gcgtcgatga
     5161 cgtaccagcg gcgttcgatg tgctcaggct tgggagagta ggtcttcatc ggaaaagagt
     5221 cggcccgaga ggccgacggc ccatgctacc tgcgggaacg gatcgtcgtc aaagctcgct
     5281 cccaggcagt gctcgggctc atcgatagct gacccgctcc aaagtgaggc cgcggggggg
     5341 cgccagaccg cccggcgcgg cggatcgatc tcgcgcggcc agcacgctcg gcatcgcggt
     5401 agcgtcctgt ttgcccgctc ccaccgccac gagcgtcccg accagcgacc tgaccatctg
     5461 gtgcaggaag gcatcggcca cggcacggac ggcgatctca tcccctgcgc gcacgacggt
     5521 cagccgccgc agatgcctca tcgtcgatcg atcgcctccg ggatgccggc agaagctcct
     5581 gaagtcgtgc ctcccttcga gatccctggc cgcccgtcgc atcggagcga gcgcgagccg
     5641 gccggggcga tgccaggcga atcggcccgt gaacgggtcc gcgagagggg cctcgtcgat
     5701 ccggtacacg tatccccggg ccgtcgcgga gaagcgggca tcgaacccgt ccgatgcgag
     5761 ccgcgcccgg acgacgacca cctcgggtgc gagcgccgag ttcagcgcct tctggagccg
     5821 cccgggcgcg accgaggtct ccgtccggaa cgatgcgacc tgaccacgtg cgtgcacacc
     5881 cgcgtcggtt cgccccgcca ccgagagctt cacgggctcc ctgagcaccc gttcgaggtg
     5941 gcgcacgagc tcgccctcga tcgtcctgat cgacgggtcc cgctgcatcg cccagcctcg
     6001 gaaggcggtg ccgtcgtagg caagcgtgag caccgtgacc gacatcagga tcggtctccg
     6061 ttcatcggga ggcgaccacg caggcgcccg ccaacgggcc gcccgggcac agcgctagag
     6121 ctcctcgcgc tccacgagct cgatcatggc catcggggcg ccgtcgccct tgcgtgggcc
     6181 gagcctcagg atcctcgtgt agccaccgtg ccgatcctcg aaccgagggc cgatctcgtg
     6241 gaacagcttc gcgacgacgt cgcggtcggg gatgtcgcgg agcacctcgc ggcgcgccgc
     6301 gaggtcaccc cgcttcgcga acgtgatcag cttctccgcg aacggccgca gctgtttcgc
     6361 cttcg
//
LOCUS       DASUVE010000245         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_869015, whole genome shotgun sequence.
ACCESSION   DASUVE010000245 DASUVE010000000
VERSION     DASUVE010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869015"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="VF036_05365"
     CDS             <1..1533
                     /locus_tag="VF036_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6264690.1"
                     /translation="RHNETGEPWDLEYRLIASDGRVVWVHDRASLIRGLERRAAAWHG
                     VMIDITAEHDAKEMLLLHKEDLERRVAERTTELQEANELMALEIGERRRMEAELRDLE
                     ERYRRLVEHMPGIAYVWDVRPGAEARTFGYVSPRIEDILGFSPEEWHASARVHPHDQA
                     RVAEAVDRSVRTGEPFVMEYRYLAKDGSVVWVLDHASLITRSDAGDPAAFQGMMLDVT
                     SRMDAESKAAAAEDRYRTLTERGPVVAYSCELSYAEGAEPSVHVSYVSPQAAELVHYP
                     IEHWRHEPEVWFGMIHPDDRERVAQTTAHTWRTGEPWMVRYRMIRSDGTLIWLLDAGR
                     LLDRDAQGRPWTFQGILLDVTTDEVARAEVEATAGAQREALEGALAIPWSETIHPDTG
                     FERYTYIGSQVFDVLGYTPEELMDEPKHFPRMVHPDDRPRVDQSVQRSDETGVWDDTY
                     RILRRDGEIRWLHSFGRRTSAPGAIPELWHGIAIDVTAYRAEAGAEAGVVADDAVVDR
                     AP"
     gene            complement(1553..>2171)
                     /gene="serS"
                     /locus_tag="VF036_05370"
     CDS             complement(1553..>2171)
                     /gene="serS"
                     /locus_tag="VF036_05370"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005452690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX6264691.1"
                     /translation="DDELYLTGTSEVPLAAFHIGEILDEADLPIRYAGYSSCFRREAG
                     SYGKDLGGMFRVHQFDKVEMFAFCTPETSWDEHERILTIEEEILGNLEIPYRVVNVAA
                     GDLGGSAAKKYDIEGWLPAQQRYRELTSCSNTTDYQARRMQTRLRRAGGDVEVVHTLN
                     GTATAIGRTLIAILENHQRADGSVEIPAKLHPYLPERARVLAPRS"
BASE COUNT          349 a          734 c          755 g          333 t
ORIGIN      
        1 cgccacaacg agacgggtga gccgtgggac ctggagtacc ggttgatcgc gagcgacggg
       61 agggtcgtct gggtccacga ccgcgcgagc ttgatccgcg gtctcgagcg ccgcgcggcc
      121 gcgtggcacg gcgtgatgat cgacatcacc gcggagcacg atgcgaagga gatgctcctc
      181 ctccataagg aggacctgga gcggcgcgtc gcggagcgca ccaccgagct gcaggaagcc
      241 aacgagctga tggcgctcga gatcggagag cgccggcgga tggaggccga gctccgtgac
      301 ctcgaggaac ggtaccgccg gctggtcgag cacatgcccg gcatcgcgta cgtctgggac
      361 gtccgtcccg gcgccgaagc ccgaaccttc gggtacgtga gcccccggat cgaagacatc
      421 ctcgggttct cacccgagga gtggcacgcg tcggcacgcg tccacccgca cgatcaagcg
      481 cgcgtggccg aggcggtcga tcggagcgtg cggaccggcg agcccttcgt catggagtac
      541 cgctacctcg cgaaggacgg gagcgtcgtc tgggtcctgg accatgcttc gctgatcacc
      601 cgatccgacg ccggcgaccc ggctgccttc cagggcatga tgttggatgt cacgagtcgc
      661 atggacgccg aatccaaggc ggcggccgcg gaggatcgct accggaccct cacggaacgc
      721 ggcccggtcg tggcgtactc ctgcgagctc tcctacgccg agggggccga gccctccgtc
      781 catgtctcgt acgtcagccc gcaggccgcg gagctcgtcc actacccgat cgagcattgg
      841 cgccatgagc ccgaggtctg gttcgggatg atccatccgg acgaccgcga acgtgtcgcc
      901 cagacgacgg cgcatacgtg gcggaccggc gagccttgga tggtccgcta ccgcatgatc
      961 cgctccgacg gaaccctcat ctggctcctc gacgccgggc gcctcctcga tcgggatgcg
     1021 caggggagac cgtggacctt ccagggcatc ctcctcgacg tgaccaccga tgaagtggcg
     1081 agagctgagg tcgaggctac cgcgggagct cagcgcgagg cgctcgaggg ggcactcgcg
     1141 atcccgtggt ccgagacgat ccaccccgac acggggttcg agcggtacac gtacatcggg
     1201 tctcaggtct tcgacgtcct cggatacacg cccgaggagc tcatggacga gccgaagcac
     1261 ttcccccgga tggtgcaccc ggacgatcgt ccccgggtcg atcagtcggt tcagcgctcc
     1321 gatgaaacgg gcgtctggga cgacacgtat cggatcctgc gacgcgacgg cgagatccgg
     1381 tggctccact cgttcggacg gcgcacctcg gctcccggcg cgatccccga gctctggcac
     1441 ggcatcgcga tcgacgtcac cgcgtaccga gcggaggcgg gagcggaagc gggagtcgtg
     1501 gcggacgacg ccgtggtcga ccgagctccc tagcgctcct tcaggcccgg ggtcaggacc
     1561 gaggagccag cacgcgtgcg cgctccggca ggtacggatg cagtttcgcg gggatctcca
     1621 cgctgccatc ggcgcgttgg tggttctcca ggatcgcgat caacgtgcgg ccgatcgccg
     1681 tggcggtgcc gttcaaggtg tggacgacct cgacgtcccc gcccgcccga cggagccgcg
     1741 tctgcatccg gcgcgcctgg tagtcggtgg tgttcgagca cgaggtgagc tcgcggtagc
     1801 gctgctgcgc cggcagccag ccctcgatgt cgtacttctt ggccgcggac ccgccgaggt
     1861 cgcccgccgc gacgttcacc acccggtacg ggatctccag gttcccgagg atctcctcct
     1921 cgatcgtgag gatgcgctcg tgctcgtccc acgacgtctc gggcgtgcag aacgcgaaca
     1981 tctcgacctt gtcgaactgg tggacgcgga acatgcctcc caggtccttg ccgtacgaac
     2041 ccgcctcgcg ccggaagcag ctcgagtagc cggcgtagcg gatcgggagg tcggcctcgt
     2101 cgaggatctc gccgatgtgg aacgccgcca aaggcacctc cgacgtgccc gtgaggtaga
     2161 gctcgtcgtc g
//
LOCUS       DASUVE010000246         5560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1247549, whole genome shotgun sequence.
ACCESSION   DASUVE010000246 DASUVE010000000
VERSION     DASUVE010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247549"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VF036_05375"
     CDS             <1..418
                     /locus_tag="VF036_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264692.1"
                     /translation="VKAAGEADIDGIATTRYTGTIDLQKVLEGFADIVGEDADAETTE
                     QLEAAVEQFEALGIDGKIPFQIWIDDEGLPRRQRITMDLGDLVPGTGDARLEMTVDYS
                     AFGEPVGVELPRASEVTDVTKMMSDAGAGEKSGVYG"
     gene            complement(401..1048)
                     /locus_tag="VF036_05380"
     CDS             complement(401..1048)
                     /locus_tag="VF036_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX6264693.1"
                     /translation="MDAALLDLYDTVARTRWRELSDRISDELGVDVAALYRAYTVTRA
                     ERSIGAFNGPEGDMTAIVEAAGFESRPELIDSLVAMERRFTETGVELWEDSLPVVFEL
                     RQRGVRTALVSNCSHSTRGIVDRLGLVDAFDEVILSFEVGLRKPDPAIYREALRRVDV
                     QPDRAVFVDDQQEYCDGAASIGIQTYLIDRTGDATPDENGHRVIRDLRALLAVDA"
     gene            complement(1071..3107)
                     /locus_tag="VF036_05385"
     CDS             complement(1071..3107)
                     /locus_tag="VF036_05385"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX6264694.1"
                     /translation="MFEELNPEQEAAVRHGEGPLLVVAGAGSGKTMTLAARVASLIER
                     GTRPERILLLTFSRRGAREMLSRAERLTAKDDAGRVWGGTFHAVGNRLLRLHGRALGL
                     TPDFTVLDQADGADVMNLLRDELGYSSRERRFPRKDTLAAIYSRTVNAGERLVDVLNG
                     HYPWCLDEAEGIVDIFRAYTERKRRQHVLDYDDLLLFWKALATSPATGPQLAAMFDHV
                     LVDEYQDTNALQADILEGMRPSEGPRNVTVVGDDAQAIYGFRAATVKNILEFPERFPG
                     TTVIKLERNYRSTPPILAASNAVIALSPQRHEKTLWSSRESELRPVLRACLDEAEQSD
                     AVCRAVLGHREVGVPLKAQAVLFRAAHHSDHLEVELARRNIPFVKYGGLKFMEAAHVK
                     DTLACLRVLENPFDEVSWFRVLQLLQGMGPASARRLMDDLEVRRTDDADAASPLARFL
                     AEPLAVPKGTAQGIRELRSVLGDCLAAETLPPAAQIARLRTFLEPVIARKYDAAAART
                     RDLEQLELLASEAASRGQFLADLTLDPPSSTGDLAGPPHLDEDYLVLSTIHSAKGLEW
                     DVVHVIHTADGMIPSDMSTGDDDEIEEERRLLYVALTRARDALHVTYPQRYYRRPRGL
                     EDPHSYSQRSRFLPDEVLLHFAVVGPQVVSVADVAEPVTAAADVDAFLAGLWSD"
     gene            complement(3154..3540)
                     /locus_tag="VF036_05390"
     CDS             complement(3154..3540)
                     /locus_tag="VF036_05390"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX6264695.1"
                     /translation="MKEGAVAVGTIDEYAKILDDWRGEVVGELDALIRRAAPGATGSI
                     KWAQPVYEQDGPFAYVKAFTSAVNFGFWRGADLTDTEGRLEGTGDRMRHVKIRGPGDV
                     DAARFTAWVKEAVTLNAEKGDPTKAR"
     gene            3572..4240
                     /locus_tag="VF036_05395"
     CDS             3572..4240
                     /locus_tag="VF036_05395"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX6264696.1"
                     /translation="MSNPEIRIEENGPYEVEGPLPIARTRTVKTEFGEPVDYAPFEPL
                     DADETYRLCRCGKSGNKPFCDDSHLEEPRVDVTERADHGPRDDRTDVFQAQDGAIISD
                     DLSICTKAGYCKDRFTGVWQMLGDPDPAVRERMGRMVQLCPSGRLAWADSVDSPDREP
                     EYEPEIGVFRNGPLFVRGGVRVVGADGEPYEVRNRQTLCRCGHSQNKPFCDGSHKLVG
                     FRDG"
     gene            complement(4237..5055)
                     /locus_tag="VF036_05400"
     CDS             complement(4237..5055)
                     /locus_tag="VF036_05400"
                     /inference="COORDINATES: protein motif:HMM:NF016334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX6264697.1"
                     /translation="MSEASARPNDLQPFLDRFVDACEQDDRVVAAFLGGSHGRGEADA
                     YSDVDLCVIAKDDAYEALVRDRAGFVGRLGAPLFLEDFGIAGLVFFALDDGNEGELFL
                     GSEGRLEELEPGPTFRTLYDPGGILEGVEFAEERTDPAAQHEVLRRILAWFWHELSHF
                     IAAMGRGELWWAAGQLESLRGHCVNLVRIEHEAFAGEEPYEKLDRTIDPAALSPIAET
                     FVPIERGALLRAARVLVRFYAEKAPGVAEANGATYPLELEELMVKRLDDLADSG"
     gene            complement(5090..>5560)
                     /gene="msrA"
                     /locus_tag="VF036_05405"
     CDS             complement(5090..>5560)
                     /gene="msrA"
                     /locus_tag="VF036_05405"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX6264698.1"
                     /translation="VEHLFRRVPGVTEAISGYSGGFVENPSDQLVCTDTTGHAEVVQV
                     TFDPEVVSYDQLLEVFWAIHDPTQVNRQGPDRGSQYRSIVLTHSDEQRAAAEASRDRV
                     QPRYERPIATAIQPFAAFYPAEDYHQRYYLNNGHEPYCHAIPPDLLEDLGLVRT"
BASE COUNT          881 a         1859 c         1961 g          859 t
ORIGIN      
        1 cgtgaaggcc gccggcgagg cggacatcga cggcatcgcc accacgcggt acaccggcac
       61 gatcgacctc cagaaggtgc tcgagggctt cgccgacatc gtgggcgagg atgccgacgc
      121 ggagacgacg gaacagctcg aagccgcggt cgagcagttc gaggcgctcg gcatcgatgg
      181 gaagatcccg ttccagatct ggatcgacga cgagggcctg ccgcgccgcc agcggatcac
      241 gatggacctc ggtgacctcg tgccggggac gggggacgcg cggctggaga tgacggtcga
      301 ctactccgcg ttcggcgagc cggtgggggt ggaacttccg agagcgtcgg aggtcaccga
      361 cgtcacgaag atgatgtccg atgcgggggc cggcgagaag tcaggcgtct acggctagga
      421 gcgcccgcag gtccctgatc acccgatgtc cgttctcgtc gggtgtggcg tcgcccgtcc
      481 ggtcgatcag ataggtctgg atcccgatcg aagcggcgcc gtcgcagtac tcctgctggt
      541 cgtccacgaa gacggcgcga tccggctgga cgtccacccg tcgcaacgcc tcgcggtaga
      601 tcgcagggtc gggcttgcgg agcccgacct cgaacgacag gatgacttcg tcgaaggcat
      661 ccacgaggcc gagccggtcg acgatcccgc gcgtcgaatg cgagcagttg ctgacgagcg
      721 ccgtcctgac gccccgctgc cggagctcga agacgaccgg cagcgagtcc tcccagagct
      781 cgacgccggt ttccgtgaac cgccgctcca tcgcgacgag cgagtcgatc agctcgggcc
      841 tcgattcgaa cccggccgcc tcgacgatcg cggtcatgtc gccctccggc ccgttgaagg
      901 cgccgatgct ccgttccgcc ctcgtgacgg tgtaggcacg gtagagcgcc gcgacgtcga
      961 cgcctagctc gtcggagatc cgatcggaca gctcgcgcca gcgggtcctc gcgaccgtgt
     1021 cgtagagatc gagcagcgcg gcatccatga cccaaggata gaggtcgagc tcagtcgctc
     1081 cagaggccgg cgaggaacgc gtccacgtcg gctgccgcgg tcaccggctc ggcgacgtcg
     1141 gcgaccgaca cgacctgcgg accgacgacc gcgaagtgga ggaggacctc gtccggcagg
     1201 aaccgcgacc gctgggaata ggaatgcgga tcctcgaggc cccggggcct gcggtagtac
     1261 cgctgcgggt aggtgacgtg gagcgcgtcg cgcgcccggg tgagcgccac gtagaggagg
     1321 cgtcgctcct cctcgatctc gtcgtcgtcc cccgtcgaca tgtcggacgg gatcatgccg
     1381 tcggccgtgt ggatcacgtg caccacgtcc cactcgaggc ccttcgccga gtggatcgtg
     1441 gagaggacga ggtagtcctc gtcgagatgc ggcgggccgg cgaggtcgcc ggtggacgag
     1501 ggcggatcga gcgtcaggtc ggcgaggaac tgcccgcggg aagcagcttc cgatgccagg
     1561 agctcgagct gttcgaggtc gcgggtacgt gcggcggccg cgtcgtactt ccgggcgatc
     1621 accggctcga ggaacgtccg gagccgggcg atctgggcag ccggagggag ggtctctgcc
     1681 gccagacagt cgccgaggac gctccgaagc tctcggatcc cctgcgccgt acccttgggg
     1741 accgcgagcg gctccgccag gaaccgtgcg aggggcgacg cggcgtcggc atcgtccgtc
     1801 cgacgcacct cgagatcgtc catcagccgg cgagccgacg cggggcccat cccctggagg
     1861 agctggagga cccggaacca gctcacctcg tcgaaggggt tctcgaggac gcggaggcac
     1921 gcgagcgtgt ccttgacgtg agccgcctcc atgaacttca ggccgccgta cttcacgaac
     1981 gggatgttcc ggcgggccag ctcgacctcg aggtggtccg agtgatgggc ggcgcggaac
     2041 aggaccgcct gcgccttcaa ggggacgcct acctcccgat gcccgaggac ggcccgacac
     2101 acggcgtcgc tctgctcggc ttcgtcgaga cacgcgcgga gcacggggcg caactcgctt
     2161 tcgcgcgagg accagagcgt cttctcgtgt cgttgcgggc tgagggcgat caccgcgttg
     2221 gatgccgcga ggatcggggg ggtcgaccgg tagttccgct cgagcttgat cacggtcgtg
     2281 ccggggaagc gctcggggaa ctccaggatg ttcttcacgg tggcggcgcg gaacccatag
     2341 atcgcttggg cgtcgtctcc cacgaccgtg acgttccgcg ggccctcaga ggggcgcatg
     2401 ccctcgagga tgtcggcctg gagcgcgttc gtgtcctggt actcgtcgac gaggacatgg
     2461 tcgaacatcg cggcgagctg cggccctgtg gccggcgagg tcgcgagggc cttccagaag
     2521 agcaggaggt cgtcgtagtc gaggacatgc tgtcggcgct tgcgttccgt gtaggcgcgg
     2581 aagatgtcga cgatgccttc tgcttcgtcc aggcaccacg ggtagtgacc attcaggacg
     2641 tcgacgagcc gctcgccggc gttcacggtc cgcgagtaga tcgccgccag ggtgtccttg
     2701 cgcgggaatc gccgctcgcg ggacgagtag ccgagctcgt cgcggaggag gttcatcacg
     2761 tcggcgccgt ccgcctggtc gaggacggtg aaatccggcg tgagaccgag cgcccggccg
     2821 tgcagacgga ggagccgatt gcccaccgcg tggaacgtgc cgccccacac acggccggcg
     2881 tcgtccttcg cggtgagccg ctcggcgcgc gacagcatct ctctcgcgcc gcgacggctg
     2941 aacgtgagga ggaggatccg ttcgggcctc gtccctcgct cgatcagcga ggccacccgc
     3001 gccgccagcg tcatcgtctt gccgctgccg gccccagcca cgaccaggag cggtccctcg
     3061 ccgtgacgga cggcggcttc ctgctccggg ttcagttcct cgaacacgcg ttcgaaggat
     3121 agtggggagg agggaccgaa tcgaggaccc gcgctagcga gctttcgtgg gatcgccctt
     3181 ctcggcgttg agcgtgacgg cttccttgac ccaggcggtg aacctcgccg cgtcgacgtc
     3241 cccgggcccc cggatcttca cgtgacgcat ccggtcgccg gttccctcga ggcggccctc
     3301 ggtgtccgtg aggtcggcac ctcgccagaa cccgaagttg acggccgagg tgaacgcctt
     3361 gacgtaggcg aagggcccgt cctgctcgta gacgggctgc gcccacttga tcgagccggt
     3421 cgcccctgga gccgcgcgcc ggatcagagc gtcgagctcg ccaacgacct cgccgcgcca
     3481 gtcgtcgagg atcttcgcgt actcgtcgat cgtgccgacc gccacggctc cctccttcac
     3541 gcggatggta cgacgtggga ggatgccgcc catgtcgaac cccgagatcc ggatcgagga
     3601 gaatggcccc tacgaggtcg aaggcccgct gccgatcgcg cggacgagga ccgtcaagac
     3661 ggagttcggc gaaccggtcg actacgcacc cttcgagccc ttggacgcgg acgagaccta
     3721 ccgcctttgt cgctgtggca agagcgggaa caagccgttc tgcgacgaca gccacctcga
     3781 ggaaccccgc gtcgacgtca cggagcgcgc ggatcatggc ccgcgcgacg accggaccga
     3841 cgtgttccaa gctcaggacg gggcgatcat cagcgacgac ctgtcgatct gcacgaaggc
     3901 cggctactgc aaggaccggt tcacgggggt ctggcagatg ctcggcgacc cggatccggc
     3961 tgtccgcgaa cggatgggtc ggatggtcca gctctgtcct tcgggacgcc tggcgtgggc
     4021 ggactcggtg gactcgccgg accgtgagcc cgagtacgaa cccgagatcg gtgtgttccg
     4081 gaacgggccg ttgttcgtgc gcggtggggt gcgggtcgtc ggagcggacg gcgagcccta
     4141 cgaggtccgc aaccgccaga ccctctgccg atgcgggcac tcacagaaca agccgttctg
     4201 cgacggctcg cacaagctcg tcggcttccg cgacggctag cccgagtcgg caaggtcgtc
     4261 aaggcgcttg accatcagct cctcgagctc gagcggatat gtcgccccgt tcgcctccgc
     4321 gacgcccggg gccttctccg catagaagcg gacgaggacc cgcgccgctc gcagcagcgc
     4381 gccgcgctcg atcggcacga acgtctccgc gatcggtgac agcgcagctg gatcgatcgt
     4441 ccgatcgagt ttctcgtacg gctcctcgcc ggcgaacgcc tcgtgctcga tccggacgag
     4501 gttcacacaa tgaccgcgga gcgactcgag ttgcccggcg gcccaccaga gctcgccccg
     4561 ccccatggcg gcgatgaagt gcgagagctc gtgccagaac cacgcgagga tccgccggag
     4621 cacctcgtgt tgcgcggcag gatccgtccg ctcctcggcg aactccacgc cctcgaggat
     4681 cccgcccggg tcgtagagcg ttcggaacgt tggaccgggc tcgagttcct ccagccgacc
     4741 ctcgctcccc aagaagagct cgccctcgtt cccgtcgtcg agggcgaaga acacgaggcc
     4801 cgcgatcccg aagtcctcga ggaagagcgg cgccccgagg cgccccacga atccggctcg
     4861 atcgcggacg agcgcttcgt acgcgtcgtc cttggcgatc acgcagagat cgacgtccga
     4921 gtatgcgtcc gcctcgccac ggccatgaga gccgccgagg aacgcggcca cgacccggtc
     4981 gtcctgctcg catgcgtcga cgaacctgtc caggaacggc tgaaggtcgt tgggtcgggc
     5041 cgacgcctcg ctcacgccgt ggagcttggc agggttcgtc ccgccggcgc taggtgcgga
     5101 cgagccccag gtcctcgagc aggtccggcg ggatcgcgtg gcaatacggc tcgtggccgt
     5161 tgttcaggta gtagcgctgg tggtagtcct ccgccggata gaaggccgcg aacggctgga
     5221 tcgccgtcgc gatggggcgt tcgtaccgag gctggacccg atcccgggac gcttccgccg
     5281 ccgcccgctg ctcgtccgag tgggtgagga cgatgctccg gtactgcgat cctcggtcgg
     5341 gtccctgccg gttcacctgg gtcggatcgt ggatcgccca gaacacctcg aggagctggt
     5401 cgtaggagac gacctcggga tcgaacgtca cctggacgac ctccgcgtga cccgttgtgt
     5461 ccgtgcagac gagctggtcg gaggggttct cgacgaagcc cccgctgtag ccggagatgg
     5521 cttcggtgac tcccgggacc cttcggaaca ggtgctccac
//
LOCUS       DASUVE010000247         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_685617, whole genome shotgun sequence.
ACCESSION   DASUVE010000247 DASUVE010000000
VERSION     DASUVE010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685617"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VF036_05410"
     CDS             complement(<1..939)
                     /locus_tag="VF036_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX6264699.1"
                     /translation="MGGPLAIVGFLALILLVVIPIHELGHLLVARRFGFKIQEYFVGF
                     GSKLWSIKRGETEYGIKAIPAGGYVKIAGMNPYETVPAQDVARAYGAKPIWQRALVIL
                     AGPLSHVLVGGLLYLTLFLTYGDPNTELVVVGEVAPTLDGSPSPALVAGLQVGDVVTR
                     VGAIEGPTPDSLGRYVTRYAEDRPGEPLTYVIERDGRSLTLEIVPRLVTEDEVARGRI
                     GFTLGAEDLSIPGSVVESGEWVGRAAWNSVTTIPKVFTVGVGRTFSVLFTDEPRRDDD
                     VTSLVGVSRQIGGAGERGDWALFLNFAAYVTIFIGVV"
     gene            complement(940..2097)
                     /gene="dxr"
                     /locus_tag="VF036_05415"
     CDS             complement(940..2097)
                     /gene="dxr"
                     /locus_tag="VF036_05415"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX6264700.1"
                     /translation="MRSVTILGSTGSVGTQALDVVRRNPERFKVIGLSAAGTNQELLA
                     GQIREFLPPVVGIADEQASEEVRTHLGSIRGVDFVVGPDAAERLASEAEADIVLNALV
                     GSSGLGPTLAALQSGKTLALANKESLVVGGELVMDLIKGEPERLLPVDSEHAALAMAL
                     RGERREDLKRVVLTGSGGPFRGWTATELAKASMKEALQHPVWSMGPKITIDSATLMNK
                     GLEVIEAHYLFGLEYSQIHVLIHPEGLVHAIAEFRDGSMRAEMATADMRLPIQLALAW
                     PERLPTGIASLPLTESPLTFEPVDHEAFPAVDLAYRVGGLGLTFPAVMNAANEVAVMA
                     FLEGKIPLPRIVEIVQTIVDEHEPPSVVSVVHIERADIWARRRTAEIIEAR"
     gene            complement(2172..>2332)
                     /locus_tag="VF036_05420"
     CDS             complement(2172..>2332)
                     /locus_tag="VF036_05420"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX6264701.1"
                     /translation="TFGDLAESLIKRDVGVKDMGSVLPGHGGVLDRIDSLLFVAPAAL
                     LLLRVIAF"
BASE COUNT          405 a          817 c          768 g          342 t
ORIGIN      
        1 caccacgccg atgaagatcg tgacgtacgc cgcgaagttc aggaacaatg cccagtcccc
       61 acgctcgccg gcgccgccga tctgccggct gacgcccacc aggctcgtga cgtcgtcgtc
      121 gcgtcgcggc tcgtcggtga acagtaccga gaacgtgcga ccgacgccca cggtgaagac
      181 cttcgggatg gtcgtcacgc tgttccatgc cgctctcccc acccactccc ccgactccac
      241 caccgacccg gggatcgata ggtcctcggc gccgagcgtg aacccgatcc ttcccctcgc
      301 gacctcgtcc tccgtgacga gacgcgggac gatctccagg gtgagggaac ggccgtcgcg
      361 ctcgatcacg tacgtgagcg gctcgccggg acggtcctcc gcgtatcggg tgacgtagcg
      421 gccgagggaa tcgggcgtcg gaccctcgat ggcgccgacg cgggtcacga catcgccgac
      481 ctgcagcccc gctacgagcg cgggcgaggg gctcccgtcg agcgtcgggg cgacctcgcc
      541 aaccaccacg agctcggtgt tcggatcccc gtacgtgagg aacaaggtca ggtacaggag
      601 cccgccgacg agcacatgcg acaacggacc ggccaggatc accagggcgc gttgccagat
      661 cggcttcgcc ccgtacgccc gcgcgacatc ttgggccgga acggtctcgt acgggttcat
      721 cccggctatc ttcacgtagc cgccggccgg gatcgccttg atgccgtact ccgtctcgcc
      781 gcgcttgatc gaccagagct tcgacccgaa gccgacgaag tactcctgga tcttgaaccc
      841 gaaccgtctc gcgaccaaga ggtggccgag ctcgtggatc gggatcacga cgagcaggat
      901 gagcgcgagg aatccgacga tcgcgagcgg gccgcccacc tagcgagcct cgatgatctc
      961 cgccgtgcgg cgacgggccc agatgtccgc gcgctcgatg tgcacgaccg agacgaccga
     1021 gggtggttcg tgttcgtcga cgatggtctg aacgatctcc acgatgcgag gcagcgggat
     1081 cttcccctcg aggaaggcca tcaccgccac ctcgttcgcg gcgttcatca ccgcggggaa
     1141 cgtgagtccc agccccccca cccgataggc gaggtccacg gccgggaagg cctcgtgatc
     1201 gacgggctcg aacgtgagcg gcgactccgt gagagggagc gacgcgatgc ccgtcggcag
     1261 ccgctccggc cacgccagtg cgagctggat cggcagccgc atatccgccg tcgccatctc
     1321 ggctcgcatc gagccgtcgc ggaactcggc gatcgcgtgg acgaggcctt ccgggtggat
     1381 gagcacgtgg atctgcgaat actccaggcc gaagaggtag tgcgcctcga tcacctcgag
     1441 gcccttgttc atgagcgtgg cggaatcgat cgtgatcttg ggccccatcg accagacggg
     1501 gtgctggagg gcttccttca tcgaggcctt ggccagctcc gtggcggtcc acccgcggaa
     1561 cgggccgccc gagcccgtga gcacgacgcg cttgaggtct tcccggcgct caccgcggag
     1621 cgccatcgcg agcgccgcgt gctcggagtc gacgggcagg agccgctccg gctcaccctt
     1681 gatcaggtcc atgaccagct cgccgccgac gacgagcgat tccttgttgg cgagggccag
     1741 cgtcttcccg ctctggagcg cggcgagcgt aggacccagt cccgacgagc ccacgagcgc
     1801 gttcagcacg atgtccgctt cggcctccga tgccaggcgc tcggccgcgt cgggcccgac
     1861 gacgaagtcc acaccccgga tcgagccgag gtgcgtccgg acctcctcgg acgcctgctc
     1921 atcggcgatc ccgacgacgg gagggaggaa ctcgcggatc tggccggcga ggagctcctg
     1981 attcgtcccc gccgcggaca agccgatgac cttgaaccgc tcgggattgc gacggaccac
     2041 atccagcgct tgggtcccga ccgagcccgt cgatccgagg atggtgacgc tccgcatggg
     2101 aacagactac ccccgtgcgc aagggaacaa ggagcaagcg gacgagaacg tcacgatccc
     2161 cgaacgggtc gctagaaggc gatgacccgc agcaggagca aggcggccgg cgccacgaag
     2221 agcagcgaat cgatccggtc gaggacgcct ccgtgtccag ggagaacgct ccccatgtcc
     2281 ttcacaccca cgtcgcgttt gatcagtgac tccgcgaggt caccgaacgt cg
//
LOCUS       DASUVE010000248         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_686572, whole genome shotgun sequence.
ACCESSION   DASUVE010000248 DASUVE010000000
VERSION     DASUVE010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686572"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /gene="ugpC"
                     /locus_tag="VF036_05425"
     CDS             complement(<1..832)
                     /gene="ugpC"
                     /locus_tag="VF036_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX6264702.1"
                     /translation="MAKIVFEGVTKEFGEEVIAVDDLSLEIADGEFMVLVGPSGCGKS
                     TILRMLAGLEDVTAGEIWIDDVQVTDLSAKERDIAMVFQNYALYPHMTVEENIAFGLK
                     LRRVAKEERVRKAREVARVLAIEHLLERKPVALSGGQRQRVAMGRAMIREPVAFLMDE
                     PLSNLDAKLRVQMRLEIQKLHRELRTTSIFVTHDQVEAMTLADRMIVMNLGVVEQIGT
                     PIDVYDDPNSLFVAGFIGSPAMNFLPGKRVGGAVDVGGGVQVPLPAGINPSGDGVTVG
                     V"
     gene            complement(837..1874)
                     /locus_tag="VF036_05430"
     CDS             complement(837..1874)
                     /locus_tag="VF036_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018531521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX6264703.1"
                     /translation="MKPPALLAVDAGGSKIDAVLLRRDGNVLGVARRRGLDHEGTGTE
                     GFLERIADAVVAACRNAGVEADHQPVAELGMYCVAGADLPADDRRIARWFRGRGLTGN
                     DVVRNDTFAVLRAGTDRTWGVAVVCGYGTNCSGISPNGRTFRFPAFGELSGDWGGGHD
                     IGTAALWYSVRAEDGRGEATTLRTLVPQHFGLTRPRQLTEAMYFDRIAERRVVELTPV
                     VFRAAAEGDAVSRAIVDRQADEIVRMVAVAVRRLHMTRLDVDVVLGGGIFSSGDTDFL
                     DRIGRGLEGIAGRARVRVLDVPPVVGAALLGLDAIGARPDAQRRARTALTKARLGAHS
                     RREQATASEKG"
     gene            complement(1871..2503)
                     /locus_tag="VF036_05435"
     CDS             complement(1871..2503)
                     /locus_tag="VF036_05435"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6264704.1"
                     /translation="MSDTTPGRRRVALTFDAEHPDRPGASAGTVGRIVETLGAAHAPA
                     TFFLQGRWSEAEPAMARRIADDGHLVGNHSHYHARMTLLSDSGIRSDVEQAEAAVSAA
                     TGVATRPWFRCPFGDGFDDPRVVDLLRALGYRNVHWHVVLEDWEPWRTAEDIARDAIE
                     GAERHGDGAVVLLHTWPDRTADALPAILDGVRASGADLVRIDDLPEAGLP"
BASE COUNT          398 a          971 c          865 g          374 t
ORIGIN      
        1 gcacgcccac ggtgacgccg tcgcccgagg ggttgatccc cgcgggcagc ggcacctgca
       61 ccccgccgcc cacgtcgacg gcgccgccca cgcgcttgcc cggcaggaag ttcatcgcgg
      121 gggagccgat gaagcccgcc acgaagagcg agttgggatc gtcgtagacg tcgatggggg
      181 tgccgatctg ctcgaccacg cccaggttca tcacgatcat gcggtcggcg agggtcatcg
      241 cctcgacctg gtcgtgggtc acgaagatgc tcgtggtgcg cagctcccgg tgcagcttct
      301 ggatctcgag gcgcatctgc acgcggagct tcgcgtccag gttggagagg ggctcgtcca
      361 tcaggaaggc gacgggttcg cggatcatcg ccctccccat cgccactcgc tggcgctgac
      421 cgccggagag ggcgaccggc ttgcgttcga gcaggtgctc gatcgcgagc acccgggcca
      481 cctcgcgggc cttacgcacg cgttcctcct tggccacccg acggagcttc aacccgaacg
      541 cgatgttctc ctcgaccgtc atgtgcgggt agagcgcgta gttctggaac accatcgcga
      601 tgtcccgctc cttggccgaa aggtccgtga cctggacgtc gtcgatccag atctccccgg
      661 cggtcacgtc ctcgagcccg gcgagcatcc gcaggatcgt cgacttcccg cagccggacg
      721 gcccgacgag caccatgaac tcgccgtccg cgatctccag cgagagatcg tcgacggcga
      781 tcacttcctc gccgaactcc ttggtcacgc cctcgaagac gatcttcgcc atcagctcat
      841 cccttctcgc tggccgttgc ctgctcgcgt cgcgaatgtg cgccgaggcg cgccttggtc
      901 agtgcggtac gtgcccgccg ctgcgcatcc gggcgcgcgc cgatcgcgtc cagacccagc
      961 agcgcggccc cgacgaccgg gggcacatcg aggacgcgga cgcgagctcg gccggcgatc
     1021 ccctcgaggc ctcggccgat ccgatccagg aagtccgtgt cgcccgaaga gaagatgcct
     1081 ccaccgagca cgacatcgac atcgagccgc gtcatgtgga gtcgtcgcac ggcgaccgcg
     1141 accatccgca cgatctcgtc cgcctggcga tcgacgatcg cccgggagac agcgtcgccc
     1201 tcggccgcag cgcggaagac gaccggcgtc agctcgacca cacggcgctc ggcgatccgg
     1261 tcgaagtaca tcgcctccgt cagctgtcgc gggcgcgtga gcccgaagtg ctgagggacg
     1321 agggtgcgga gcgtcgtggc ctcgcctcgc ccgtcctccg ctcgcaccga gtaccagagg
     1381 gctgcggtcc cgatgtcgtg acccccgccc cagtcacccg acaactctcc gaaagccggg
     1441 aaccggaacg tcctcccgtt cggcgagatc cccgagcagt tggtgccgta gccacaaacg
     1501 acggccacgc cccacgtccg atccgtgccc gcacggagga ccgcgaacgt gtcgttgcga
     1561 acgacgtcgt tcccggtcag accgcgacca cggaaccacc gtgcgatccg gcggtcgtcc
     1621 gcgggcagat cagcacccgc gacgcagtac atccccagct cggcgaccgg ctggtgatcc
     1681 gcttcgaccc ccgcgttccg gcaggccgcg accaccgcgt cggcgatccg ttccaagaag
     1741 ccctcggtgc cggtcccctc gtggtcgaga ccacgccgcc gggcgacgcc cagcacgttc
     1801 ccatcgcgcc gcagcaggac ggcgtcgatc ttcgaaccgc cggcatccac cgcgagcagc
     1861 gcgggcggct tcacgggaga ccggcctcgg ggaggtcgtc gatccgaacc agatccgcac
     1921 cagatgcacg caccccatct aggatcgccg gcagcgcatc ggccgtccga tccggccacg
     1981 tgtggaggag gaccaccgcg ccgtcgccgt gacgctccgc gccttcgatc gcatcacgtg
     2041 cgatgtcctc ggccgtccgc cacggctccc aatcctccag gaccacgtgc cagtgcacgt
     2101 tccggtagcc gagcgcccgg agcaggtcga cgacgcgcgg gtcgtcgaac ccgtcgccga
     2161 acgggcatcg gaaccatggt cgcgtcgcca ccccggtcgc cgccgagacg gccgcctccg
     2221 cctgttccac gtccgagcgg atcccgctgt cggagagcag cgtcatccgc gcatggtagt
     2281 gagagtggtt gccgaccagg tgaccgtcgt cggcgatccg gcgggccatc gccggctccg
     2341 cctccgacca ccgcccctgc aggaagaacg tggccggcgc gtgggcggcc ccgagggtct
     2401 cgacgatgcg gcccaccgtc ccggcgctcg ccccggggcg atccgggtgc tcggcgtcga
     2461 acgtcagggc gacgcgacgg cggccaggtg tcgtatcact cacgcgtggt caccttggtc
     2521 gcgtggagga acgtgcccgg ctcgcgaccg cgggcgatcg cgagcgcgtg cgccagcagt
     2581 tgggtgggca cgatcgaggc ggcgggcg
//
LOCUS       DASUVE010000249         3421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_499613, whole genome shotgun sequence.
ACCESSION   DASUVE010000249 DASUVE010000000
VERSION     DASUVE010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499613"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VF036_05440"
     CDS             <1..649
                     /locus_tag="VF036_05440"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEX6264705.1"
                     /translation="HDRSRADRRYGVRLVYEPGEGIVLREVWRGRVFEARPTTVVRDD
                     PDQLILLLPGGARCGVPIGADGFELRLPDRPWRLEIRPRGDRSILSFAWPATPYSVLL
                     WTARDGRRVWYVNLQDPLTRTPIGFDTVDHALDVVIELDRSSWRWKDEEELAEAVTDG
                     LFTAQEAADFRAWGLRAVDRVMSKSPPFDHDWEGWRPDPEWAVPELQMGWDVDPG"
     gene            724..1974
                     /locus_tag="VF036_05445"
     CDS             724..1974
                     /locus_tag="VF036_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEX6264706.1"
                     /translation="MREPVFVFDLEAVRARFPALARVGDDGRPPILVDAPGGSQVPLD
                     VIEAVSRHYRRGMSNTHGAFATSEETDAVIGEARRAAADFSGADPDEIVFGPNATSLL
                     LHVSRSFAKTLEPGDEVVVTRLDHDANVRPWVLAAADAGATVRWVDVHEDDVTIDVAS
                     FDAALSPRTKLVAFTLASNAVGTIPAAADLVTRAKAVGAVVALDGVHLAQHRLPDLHG
                     LGADLLVCSPYKFFGPHLGVLAARRRILASWSPYKLIPSSDDVPERWETGTQNHEGLA
                     GLTAAVDYLAGLASVSGTGRRDRLAAAYEAITAHERMLADRFLVGLASLPSVRLWGIA
                     DRDRIAERTPTFAIRVGDEHPAETAKALGARGIFVWDGDYYAREIMVRLGLFDTGGAV
                     RIGFCHYHSPHEVDRVLDALRELG"
     gene            1984..>3421
                     /locus_tag="VF036_05450"
     CDS             1984..>3421
                     /locus_tag="VF036_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264707.1"
                     /translation="MRSPEADAPRDRSPVVALLLAALAIAFVAILLLLPYAGREEPGQ
                     SYDTPYYVWRTRAVASDGLGVLTTIPTGAVPERPGVPVLGATLGDVIGSDALTYTVIL
                     RAVAAVAIGLAAGAMALEALGEGRWAFAAFVAGLGASAAVVGTAVGSLDQLLVEVLIV
                     AAAAVVPLVVAGRRGLLGVGVLSAAAAATHWVFAALFLLLLAAVALARVPGSVAARRS
                     GAGWATTPSVRLLRIVLVASAAAVATLALLPTLPDHLPPAIGDRGNQLRLAAYELSLV
                     LPLAAFGFVLTFPRSEGSRRTTLVLLGLWAATVPVAIAVSALLPTPIKLFRVAPFALG
                     VPALVTLGLVTVADRAGSRFGRVGTVVGALVLAGGVVWASGAPVSSFAGSGAASIAER
                     MAQARTAGQYLDGIPRSDRPVIFLTQGNPRLLDRAVRSAVPPDLIPDSWVFVGTPDDL
                     AARGPVEDPDRPRLSALAQKWWAVAWPNP"
BASE COUNT          396 a         1231 c         1231 g          563 t
ORIGIN      
        1 gcacgatcgg agtcgagccg atcggagata cggtgtgcgc ttggtctacg agcccggcga
       61 ggggatcgtg ctccgggagg tgtggcgtgg ccgtgtcttc gaagcgcggc cgacgaccgt
      121 cgtccgggac gaccccgatc agttgatcct cctgctgccg ggcggcgctc gttgcggcgt
      181 tccgatcgga gccgacgggt tcgagctcag gctccccgac cgcccgtggc gtctcgagat
      241 ccggccacga ggcgaccgat cgatcctgtc cttcgcctgg ccggcgacgc cttattccgt
      301 cctgttgtgg accgcccgcg acggtcgccg ggtctggtac gtcaacctgc aggaccccct
      361 cactcgtact ccgatcgggt tcgacacggt cgatcacgcg ctggacgtgg tgatcgagct
      421 cgatcgttct tcgtggcgat ggaaggacga ggaggagctc gccgaggcgg tcaccgacgg
      481 gctcttcacc gcccaggagg cggcggactt ccgagcctgg ggtctcaggg cggtcgatcg
      541 ggtcatgtcg aagagtcccc cgttcgacca cgattgggag ggctggcgcc ccgacccgga
      601 gtgggccgtt ccggagctcc agatggggtg ggacgtcgat ccgggatgac cgaccgggca
      661 gcgccctagg ctgtaacgtg cctcggaccg accgagtcgg cctcggttgc cgagatcgac
      721 ccggtgcggg agcctgtgtt cgtgttcgac ctcgaggcag tccgcgcccg cttcccggcc
      781 ctcgcccgcg tcggcgacga cggccggccg ccgatcctcg tggacgcccc cggtgggtcc
      841 caggtgcccc tggacgtgat cgaggcggtg tctcgccact accgccgcgg gatgagcaac
      901 acgcacgggg cgttcgccac gagcgaggaa accgacgccg tcatcggaga ggctcgccga
      961 gcggccgcgg acttctcggg agcggatccc gatgagatcg tcttcggccc gaacgcgacg
     1021 agcctgctgc tccacgtctc cagatcgttc gcgaagaccc tggagccggg ggacgaggtc
     1081 gtcgtcaccc ggttggacca cgatgcgaac gtccgaccgt gggtgctcgc ggccgcggac
     1141 gccggcgcca ccgttcgatg ggtggacgtc cacgaggacg acgtcacgat cgacgtcgcg
     1201 tccttcgacg ccgcgctgtc gccgcggacg aagctcgtcg cgttcacgct cgcgtccaac
     1261 gcggtcggca cgatccccgc cgcggccgat ctggtcaccc gcgcgaaagc ggtgggcgcc
     1321 gtcgtcgcgc tcgacggggt ccatctcgcg cagcaccgtc tccccgacct ccacgggctc
     1381 ggcgccgacc tgctcgtgtg ctccccgtac aagttcttcg ggccgcacct cggtgtcctc
     1441 gcggcgcggc ggaggatcct cgcgtcctgg tcgccgtaca agctgatccc gtcgtcggac
     1501 gacgtccccg agcgatggga gaccgggacc cagaatcacg aagggctcgc cggcctcacc
     1561 gcggccgtgg actacctcgc ggggctcgcc tccgtctccg gcaccggtcg gcgggatcga
     1621 ctcgctgccg cctacgaggc gatcaccgca cacgagcgga tgctggcgga tcggttcctc
     1681 gtcggtctcg cgagcctccc ctcggttcgc ctgtggggca tcgccgaccg cgatcgcatc
     1741 gctgagcgga cccccacgtt cgcgatccgg gtgggcgacg agcatccggc cgagacggcg
     1801 aaggcgctcg gagcgcgcgg catcttcgtc tgggacggcg actactacgc ccgcgagatc
     1861 atggtccgcc tcggactgtt cgacacgggg ggcgccgtcc gcatcggctt ctgtcactac
     1921 cactcgcctc acgaggtgga tcgggtgctg gacgcgctgc gcgagctcgg ctagcgacgt
     1981 cgggtgcgat cgccggaggc cgatgcaccg agggatcgga gtccggtcgt cgcgctgctg
     2041 ctcgccgcgc tcgcgatcgc gttcgtggcc atcctgctcc tgctccccta cgcgggacgg
     2101 gaggagcccg ggcagtcgta cgacaccccg tactacgtgt ggcggacgag ggcggtcgcc
     2161 tcggacgggc tgggtgtcct gacgacgatc cccacggggg ctgtcccgga gcggccgggc
     2221 gtgccggtgc tcggagcgac gctcggcgac gtgatcggct cggacgccct cacgtacacc
     2281 gtgatcctgc gcgcggtcgc tgcggtcgcg atcggcctcg ccgcaggtgc gatggccctc
     2341 gaggctctcg gggaggggcg atgggcgttc gcggcgttcg tggccggcct cggggcgtcc
     2401 gccgcggtcg tgggcacggc ggtcgggagt ctcgaccaat tgttggtcga ggtcctcatc
     2461 gtcgccgccg cggcggtcgt cccgctggtg gtcgcggggc gtcgcggcct gctcggcgtg
     2521 ggcgtcctct ccgctgccgc ggcggcgacc cactgggtgt tcgcggccct cttcctcctc
     2581 ctcttggcgg cggtcgcgct cgcgcgcgtg cccggatccg tggccgcccg ccggtcgggg
     2641 gcgggatggg cgacgacgcc gtcggtccgt ctcctccgta tcgtgctcgt ggcctcggcc
     2701 gcagcggtcg ccaccctcgc cctcctaccg acgctccccg accacctgcc cccggcgatc
     2761 ggcgatcgag ggaaccagct ccgcctggcg gcgtacgagc tctccctcgt cctcccgctc
     2821 gcggcgttcg gcttcgtcct gacgttcccg cgatccgagg gttcgcgcag gacgaccctc
     2881 gtcctcctcg gcctgtgggc ggcgaccgtg ccggtcgcga tcgccgtctc ggcgcttctg
     2941 cccaccccga tcaagctctt ccgcgtggct ccgttcgcgc tcggagtgcc cgcgctggtc
     3001 accctcggcc tggtgacggt cgcggaccgc gctggctctc gcttcggccg ggtggggacg
     3061 gtcgtcggcg ccctggtgct cgccggagga gtcgtctggg cgagcggggc tccggtgtcc
     3121 tcgttcgcgg gatcgggggc ggcgtcgatc gcggagcgga tggcgcaagc gcggaccgcg
     3181 ggccagtacc tcgacgggat ccctcggtca gacagacccg tgatcttcct gacgcagggc
     3241 aacccacgac tcctcgaccg tgcggtgcga tcggccgttc cacccgacct gatccccgat
     3301 tcgtgggtct tcgtcgggac gcccgacgac ctcgccgcgc gcgggcccgt ggaggatccg
     3361 gaccgcccac ggctctctgc cctggcgcag aagtggtggg ccgtcgcgtg gccgaacccg
     3421 t
//
LOCUS       DASUVE010000250         2776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1440729, whole genome shotgun sequence.
ACCESSION   DASUVE010000250 DASUVE010000000
VERSION     DASUVE010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440729"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..896)
                     /locus_tag="VF036_05455"
     CDS             complement(18..896)
                     /locus_tag="VF036_05455"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6264708.1"
                     /translation="MNLAASKGGKGVLWVAFWLLVTFLYAPILILGIFSFNDRDIVSF
                     PWQGFTTRWYQGLFDNPQLLEALRTSAWIALVASVITTVLAIPAAIALQRRRFRGKAV
                     ASGFMLAPLVVPLVVLGTAMLLLFNYVGIPFGPITLIIAHVIIALPFAILTLTPRLER
                     IPVAVEEASRDLGAGAVTTFRRITFPLLAPAVVSAFLIVYVISFDEIVLASFLAGTTT
                     TFPLYVYSQLRLPRQLPQIPAIAVIVLLVSTILVVAAEVLRARSDRLLGEGQRRVDED
                     DAATAVSASLGAGGSL"
     gene            complement(893..1816)
                     /locus_tag="VF036_05460"
     CDS             complement(893..1816)
                     /locus_tag="VF036_05460"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6264709.1"
                     /translation="MATAEPTASRRRRRGGNSASTWAIVGVPLLWVTVFFVVPVIMIA
                     VYSVGGISGLSPNDIEHFTLTPWKDFMGSSTYMGGVNPLGSGLFWKSIRMSLVVSITA
                     LVLAYPVAYLLAMLAGRRKYVLLLVIIVPFLTSYLLRVLAWRVLLNPQGVINTFLEDT
                     LGVTDQPITWLFNSRFAIYLVLSYVWVPFVALPIFVTLEGIDEHLLEAGSDLGASRWR
                     IFWTVTFPLSIPGVIAGFIFVFIPTIGEYVTPQLVGGPSGFMFGSAIQSAFTQGLDWQ
                     FGSAMAMFLVFAVAVLLAIFGRWLNVRSVAA"
     gene            complement(1833..>2776)
                     /locus_tag="VF036_05465"
     CDS             complement(1833..>2776)
                     /locus_tag="VF036_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264710.1"
                     /translation="WIWKEYANKGYPEPEFSFLTNTEGVIAKTQGGFYWDITHPETGY
                     IQDYINMDVLQPWDTSLIPNFTDLSPQLEAYGAVNGQQYEIVTDWGYSGVIIREDLVP
                     DPNLNSYSYLFDDALKGHIGWFDTPWILQQAAMVQGVDAAESFAMTDEQLNMAKEYAI
                     SKAGNVYTIWVDFTAMWDDVRQGNIWASYSWPDTYVVLKEEVPVKYVRPKEGVLSWAE
                     GLVLRKDTEQYHHAHAFADAWASLATATRLITSWGYGHSNVNVDLDAIDPDVVAVFGL
                     DDPATNLAEPVSYFDRYQPDRDRYNRAWDEVKASIGA"
BASE COUNT          507 a          930 c          928 g          411 t
ORIGIN      
        1 gatcgcgggt ccgtcgttca caggctccct ccggctccga gcgacgcgct cacggcggtc
       61 gcggcgtcgt cctcgtccac gcgtcgctgc ccctcgccga gcagccgatc cgagcgggct
      121 cgcaggacct cggccgccac gacgaggatc gtcgagacca acagcacgat caccgcgatc
      181 gcggggatct ggggcagctg ccgcggcagc cgcagctgcg agtacacgta cagggggaag
      241 gtcgtcgtcg tcccggccag gaacgacgcc agcacgatct cgtcgaacga gatcacgtac
      301 acgatcagga acgccgacac cacggcgggc gcgagcagcg ggaacgtgat ccgccggaac
      361 gtcgtcaccg cgccggcgcc caggtcgcgg ctcgcctcct cgacggcgac ggggatccgt
      421 tcgagccgcg gcgtgagcgt caggatcgcg aacggcagcg cgatgatcac gtgcgcgatg
      481 atcagcgtga tcggcccgaa cgggatcccg acgtagttga agagcagcag catcgcggtg
      541 ccgagcacga cgagcggcac gacgagcggc gcgagcatga agcccgaggc gaccgccttg
      601 ccacggaagc ggcgccgctg cagcgcgatc gccgcgggga tcgcgagcac cgtcgtgatg
      661 acgctcgcga cgagcgcgat ccacgcgctc gtgcggagcg cctcgaggag ctgcgggttg
      721 tcgaacagcc cctggtacca gcgggtcgtg aagccttgcc atgggaacga gacgatgtcg
      781 cgatcgttga agctgaagat gcccaggatc aggatcggcg cgtagaggaa ggtcacgagc
      841 aaccagaacg ccacccacag gacgcccttg ccacccttgg acgccgccag gttcacgcgg
      901 ccacgctccg cacgttgagc cagcggccga agatcgcgag caggaccgcg accgcgaaca
      961 cgaggaacat cgccatcgcc gagccgaact gccagtccaa cccctgcgtg aacgcgctct
     1021 ggatcgcgct cccgaacatg aaccccgatg gtccgccgac gagctgaggc gtgacgtact
     1081 caccgatcgt cgggatgaac acgaagatga accccgcgat cacccccggg atcgacagcg
     1141 ggaacgtcac ggtccagaag atccgccacc gcgacgcgcc gaggtccgac cccgcctcga
     1201 ggaggtgctc gtcgatcccc tcgagcgtca cgaagatcgg gagcgccacg aacggcaccc
     1261 agacgtagct cagcacgagg tagatcgcga acctgctgtt gaacagccac gtgatcggct
     1321 gatccgtcac gccgagcgtg tcctccagga aggtgttgat cacgccttgc gggttcagca
     1381 ggacccgcca ggcgagcacc cggagcaggt agctcgtcag gaacgggacg atgatcacca
     1441 acaggagcac gtacttccgg cgtccggcga gcatcgcgag caggtacgcg accgggtacg
     1501 cgagcacgag cgcggtgatc gaaacgacca gcgacatccg gatcgacttc cagaacaacc
     1561 ccgaccccag ggggttgacg ccgcccatgt aggtggagga ccccatgaag tccttccagg
     1621 gcgtcagcgt gaagtgctcg atgtcgttgg gcgacaggcc ggagatgcca ccgacgctgt
     1681 agaccgcgat catgatcacc ggcacgacga agaacacggt gacccacagc agcggcacgc
     1741 cgacgatcgc ccacgtcgag gcgctgttgc cgccgcgacg ccggcgacgc gacgcggtcg
     1801 gttcggcggt ggccacgtcc gatccggtgg tgctaggcgc cgatcgacgc cttgacctcg
     1861 tcccacgccc ggttgtatcg gtcgcgatcg ggctggtacc ggtcgaagta gctcaccggc
     1921 tcggccaggt tcgtcgccgg atcgtccagc ccgaacaccg ccacgacatc ggggtcgatc
     1981 gcatcgaggt cgacgttgac gttcgagtgg ccgtagcccc acgacgtgat caggcgggtg
     2041 gcggtggcca ggctcgccca ggcgtcggcg aacgcgtgcg cgtggtggta ctgctcggtg
     2101 tccttgcgga gcaccaggcc ctcggcccag gagagcacgc cctccttggg ccgcacgtac
     2161 ttcaccggga cctcctcctt cagcacgacg taggtgtcgg gccatgagta gctcgcccag
     2221 atgttgccct gccgcacgtc gtcccacatc gccgtgaagt cgacccagat cgtgtacacg
     2281 ttgccggcct tcgagatcgc atactccttc gccatgttga gctgctcgtc ggtcatcgcg
     2341 aagctctccg cggcgtcaac gccctgcacc atcgcggcct gctgcaggat ccacggcgtg
     2401 tcgaaccagc cgatgtggcc cttcagcgcg tcgtcgaaca ggtaggagta ggagttcagg
     2461 ttcgggtcgg ggaccaggtc ctcgcggatg atgaccccgg agtagcccca atcggtcacg
     2521 atctcgtact gctggccgtt caccgcgccg taggcctcga gctgcgggct caggtcggtg
     2581 aagttcggga tcagcgaggt gtcccagggc tgcaggacgt ccatgttgat gtagtcctgg
     2641 atgtagcccg tctcggggtg ggtgatgtcc cagtagaagc cgccctgggt cttcgcgatc
     2701 acgccctcgg tgttcgtgag gaacgagaac tcgggctcgg ggtagccctt gttcgcgtac
     2761 tccttccaga tccacg
//
LOCUS       DASUVE010000251         4019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_688337, whole genome shotgun sequence.
ACCESSION   DASUVE010000251 DASUVE010000000
VERSION     DASUVE010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688337"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VF036_05470"
     CDS             <1..654
                     /locus_tag="VF036_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEX6264711.1"
                     /translation="WGLSTFYVVSLTSLLLGSAFAIQRFGHLEVADARRYLDLFFMGA
                     AFLLLETKSIVQFALLFGTTWLVNSLVFAGILLSVLLAIEIAERVKRLRSGLLYSALL
                     VSLGVAWVVRPEQLLGLSPVTRFVAATTITFAPIFFGNLIFAERFRSVGSSTTAFGIN
                     LLGAILGGVLEYSAVVLGYRGLILVVAAAYTAAYVLRPAEGAPQEPGVLETAGERTG"
     gene            784..1575
                     /locus_tag="VF036_05475"
     CDS             784..1575
                     /locus_tag="VF036_05475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6264712.1"
                     /translation="MTRAGSGDSLAAMPEEYTDATYGDRIADIYDVRYLEAFVEDTAD
                     AVSFLKTLAEEGPALELGIGTGRVAIPLAEAGVEVHGIDASEAMVAKLRSKPVGDSIQ
                     VTVGSFADFSVEARFPLVYVVFNTFFSLLTQDEQVSSFASVARHLTPGGAFVMQAFVP
                     DVARFDSHQQRVAVESVGIDEITLEMSSHDVFAQRTDSQYVLLRQGDVRLFPVRIRYA
                     YVSELDLMARLAGLRLRERWASWRREAFPSAEWSHISVWEPEGRS"
     gene            2099..2338
                     /locus_tag="VF036_05480"
     CDS             2099..2338
                     /locus_tag="VF036_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264713.1"
                     /translation="MALFGDVRMDLRSRPPTDGATLRVFHLVGDVNLRVSTGTRVVTG
                     GTTLFGDQRVEVGHGEGPEFEVRAWGLVGDVHVTE"
     gene            2600..3328
                     /locus_tag="VF036_05485"
     CDS             2600..3328
                     /locus_tag="VF036_05485"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX6264714.1"
                     /translation="MPTGDQDQPPHSRAEQIRKREFSTVRRGYHPDEVRAYLVSIADL
                     VAAMAEELRLSRESQQAAAAAATPAPDDPYEALSRRFATLIEKADQEATRTIEEARSQ
                     ATRELEDARKEAHRITVDAQAHAEVTRHEVADLMERAMTEADRILSRAAARRKSLVMQ
                     LEEMHGKLIAVAEDLAVPVDEAGLDDLDDDELAKRRDQRQTPRQLTGTAPDPRTEELW
                     GQKDVPIELPDLASLDLDIDERDE"
     gene            3347..>4019
                     /locus_tag="VF036_05490"
     CDS             3347..>4019
                     /locus_tag="VF036_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264715.1"
                     /translation="MASILVVCSGNLCRSPFAAAVLGRMLTERVGPSATEVSSAGTIA
                     HAGEPATREAIAVASEHGVDVSSHRARPLTREDLAGADLVLAVSTEHRDEVARLDPRA
                     ATKTFTVKELVRLLGDLPSPERGAGREWLTARIAEAQAHRRAGFDGNPDDEDIADPLG
                     HPLGTYRRRLSRPVRPEPAAGRDFPRVTGAHGGAARIPGLRHILTAHPSVDYPFVADV
                     APLVFD"
BASE COUNT          630 a         1324 c         1353 g          712 t
ORIGIN      
        1 tgggggctat cgacgttcta cgtcgtttcg ctgacctccc tgctcctcgg ttctgcgttc
       61 gcgatacaac gcttcggaca tttggaggtg gccgacgcgc gccgctacct ggacctcttc
      121 ttcatgggag cagcgttcct cctgctggaa acgaagagca tcgtgcaatt cgctctcctc
      181 ttcgggacga cgtggctcgt caattcactc gtcttcgccg gcatcctgtt gtccgtcctt
      241 ctcgctatcg agatcgcgga gagggtgaag cggctgcgtt ccgggctcct ctactcggcg
      301 ctgctcgtct ccctgggagt ggcatgggtc gtcagacccg agcaactgct cggtctctcc
      361 ccggtgacca gattcgtggc ggcgacgacc atcacgttcg ccccgatctt cttcgggaac
      421 ctcatcttcg cggagcggtt ccggtccgtc ggttcctcaa cgacggcctt cgggatcaac
      481 ctcttgggag cgatcctcgg cggcgtcctc gagtacagcg ccgtcgtcct cggatatcgg
      541 gggctgatcc tagttgtcgc tgccgcctac accgccgcct atgtgttacg cccagccgag
      601 ggcgctcctc aagagcccgg tgttcttgaa actgcaggtg aacgaaccgg ataagaccac
      661 agccgactgg gtcgtgcgta ccacgacgcg gttcaccgct tggtgcaacc cgagagcgtc
      721 gcgggttgaa gccttggtcg ggtcgcccat gtcgggtccg agggatctgt caaggatggc
      781 cggttgacgc gtgccggctc gggtgattcc ctcgcggcga tgcccgagga gtacacggac
      841 gccacgtacg gagaccggat cgccgacatc tacgacgtcc gctacctcga ggcgttcgtc
      901 gaggacaccg cggacgcggt ctcgttcctg aagacgctcg ccgaggaagg ccctgcgctc
      961 gagctcggca tcggcaccgg acgagtggcg atcccgctgg ccgaggccgg cgtcgaggtg
     1021 cacggtatcg atgcctccga ggcgatggtg gcgaagctgc ggtcgaagcc cgtcggcgat
     1081 agcatccagg tcacggtcgg cagcttcgcg gacttctccg tcgaggcccg cttccctctc
     1141 gtgtacgtcg tgttcaacac cttcttctcg ctcctgacgc aggacgagca ggtgtcgagc
     1201 ttcgcgtcgg ttgctcggca cctgaccccc ggcggggcct tcgtgatgca ggccttcgta
     1261 cccgatgtcg cgaggttcga cagccatcaa cagcgagtgg ccgtggagtc ggtaggcatc
     1321 gatgagatca cgctcgagat gtctagccac gatgtcttcg ctcagcggac cgacagccag
     1381 tacgtcctgc tccgccaggg ggacgtccgg ctcttcccgg tacggatccg ctacgcgtac
     1441 gtctcggagc tcgatctgat ggctcgtctg gccggcctgc ggctccgcga gcggtgggcc
     1501 agctggaggc gcgaggcgtt cccctcagcg gagtggtccc acatctccgt gtgggagccc
     1561 gagggccgat cctgaaccgc agggatcgcg cggctcctgc tcgctagact cggatcgatc
     1621 aatgagctgg gaggagacct cgatctcgcc ccccggccct cggccctcgt cctcgtcggg
     1681 ccggtaggga accgctctga agcaggtcag gtgagtcgga ctgacggttt cgggaacgga
     1741 tccacggcat ctcggaacgc gaggtcccgg tagttgcgga actcagccgc acgtggcctg
     1801 atctgcgcgc cgcccggcga cggagcgatt cgacctgtcc gaggccccac ttctctcttt
     1861 ttttttgacc tccccatccc gatcggacca gacttggcgt atgcaggccg acgtgagctt
     1921 ctccccgggc ccggccttgt gggtgatcct cggggtcatc gttctgtggc tccttgcacg
     1981 atggctctgg gtcaggcgct cgcggaaggc aggatcctcg gcgagacctc cagcgttctc
     2041 tctcttcggc gatgtgaccg agagcgtgct tcccaccgga tggtcggatc agcagatcgt
     2101 ggccctcttc ggtgacgtgc ggatggatct gcgcagtcga cctcctaccg acggagccac
     2161 gcttcgcgtc ttccatctgg taggcgacgt caacctgcgg gtctctactg ggacccgggt
     2221 tgtcaccggg ggcaccaccc tcttcggcga tcagcgagtg gaagtgggac acggcgaagg
     2281 tcccgagttc gaggtgcgcg cctggggcct cgtcggcgac gtgcacgtca ccgagtagag
     2341 acccgtggtt ggaggtgggt ccgagcgccg acgcgagacc cgtttgtctt cgacgtgaca
     2401 cttcgacggc cgacatgctt cgttcccacg cgagggctag tcgctggctt tcgcgaggat
     2461 gaaaggcgat ctgatcacgg tcgagtggtg tacgacgatg atctccgagg gcggggaggt
     2521 cacgcttcag gggccgaggg cgcactcgag tcatcacccg ccagggtggt acggtggcac
     2581 tcgaccccgg gaggtcacga tgccgacagg cgaccaggat cagccgccgc actcgcgcgc
     2641 ggagcagatc cgaaaacgtg agttctccac ggtccggcgg ggttaccatc ccgacgaggt
     2701 tcgcgcctac ctcgtctcga tcgcggacct ggtcgcagcg atggcggaag agctgcgtct
     2761 ttcgcgcgag agccagcagg cggccgccgc agcggcaacg cccgcgcccg acgatccgta
     2821 cgaagcgctg tcgaggcgtt tcgccacgct gatcgagaag gccgatcagg aagccacaag
     2881 gaccatcgaa gaggcgaggt cgcaagcgac ccgagagctc gaggatgctc ggaaggaggc
     2941 ccaccggatc acggtcgacg cgcaggcaca cgcggaggtg acccggcacg aggtcgctga
     3001 cctgatggag cgagcgatga cggaggcgga tcggatcctc tcccgagccg ccgcgcgccg
     3061 gaagagcctc gtcatgcagc tcgaggagat gcacgggaag ctcatcgcgg tggcggagga
     3121 tctcgccgtc ccggtcgatg aggccgggct cgacgacctg gatgacgacg agctggcgaa
     3181 gcgccgtgac cagcgccaga cgccccgcca gctgaccgga acggcccccg accctcgaac
     3241 cgaggaactc tggggccaga aggacgtgcc gatcgaactc ccggatctcg cgtccctcga
     3301 cctggacatc gacgaacggg acgagtaggc cgcctatcct gacgtcgtgg catcgatcct
     3361 cgtcgtgtgc tcgggcaacc tgtgtcggtc ccccttcgct gcagccgtcc tggggaggat
     3421 gttgaccgag cgggtcggcc catcggcgac cgaggtgtcc tccgccggaa cgatcgccca
     3481 cgccggggag ccggcgaccc gcgaggcgat cgccgtcgca tccgagcacg gggtcgacgt
     3541 ctcctcgcac agggcacgcc cgctcactcg tgaggatctt gccggggccg acctggtgct
     3601 cgcggtgtcg acggagcacc gggacgaggt cgcgcggttg gacccacggg cggccacgaa
     3661 gacgttcacc gtgaaggaac tggtgaggct gctcggggac ctgcccagcc cggagcgcgg
     3721 tgcgggacgg gagtggctca cggccaggat cgccgaagcg caggcgcatc gccgggcggg
     3781 gttcgacggc aacccggacg acgaggacat cgccgaccct ctgggccatc cgctcgggac
     3841 gtaccgtcgt cgcctatcac gacccgttcg tcccgagccc gcagctggcc gggacttccc
     3901 tcgagtcaca ggagctcacg gaggagctgc tcgcatccca ggactgcgtc atatcctcac
     3961 cgcgcacccg tcggtggact acccgttcgt cgccgatgtg gctccgctgg tgttcgacg
//
LOCUS       DASUVE010000252         4373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_876835, whole genome shotgun sequence.
ACCESSION   DASUVE010000252 DASUVE010000000
VERSION     DASUVE010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876835"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VF036_05495"
     CDS             <1..1081
                     /locus_tag="VF036_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6264716.1"
                     /translation="VGGGPMGTAAARSLGARGRAVILIERFTLGHARGSTAGTTRNFR
                     LTYHDPLYVRMARRSLERWRELEDEAGVELLRVVGGLDVGAATEASARALEHAGERLE
                     RPSDAEVAERWPVLRFPPRSSFVYQPDGAVLRSQEAVRTLALLARAHGAEVREETAAG
                     SIAASGDGVEVVTASSTIRAPVAIVAAGAWSGPLLGQAGIRLTLQPTLEQSTYFGADA
                     TDLPTVIDWDETPEQPPYIVPDPFEPGTIKAGAHLSGPVVDPDTRSFDADETRERQVA
                     DWVGRRLVSGARPLRTETCLYTRTPDEDFVIDRVGPVVVASPCSGHGFKFTPLIGEVL
                     ADLVIGDEPEIPLERFRADRPALRG"
     gene            complement(1089..1523)
                     /locus_tag="VF036_05500"
     CDS             complement(1089..1523)
                     /locus_tag="VF036_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6264717.1"
                     /translation="MTEPQLGRQLLAAHRALAAELVVELEERGWPELRASQASLVLNV
                     DRRSGTRLTELARRAGVTKQAMMVVVDELEVRGFVRRTPDPDDGRAKVVRLTARGRTL
                     GAECRRAVAAVEARTRRTLGGRRYEGLREALDLLLETEEETA"
     gene            1618..2136
                     /locus_tag="VF036_05505"
     CDS             1618..2136
                     /locus_tag="VF036_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6264718.1"
                     /translation="MEVREATPADYAEAGRVTADAYREYVRPGEEDWERYLLRIADVR
                     ARADQTTIMVAVEDDRILGSLTLELDGRVRDGEQEHRPLDPGEAHIRMLGVDPTARSR
                     GVARALMAASEARAHEAGKTLMTLHTTQRMGAAQAMYERLGYERLEDRVYPDGFVLLT
                     YRKPLEVSGSRA"
     gene            complement(2276..4234)
                     /locus_tag="VF036_05510"
     CDS             complement(2276..4234)
                     /locus_tag="VF036_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6264719.1"
                     /translation="MGRHGFFRVSPIDEDHRKVDRATVRRVVGTFRPYKGKVGLVGLM
                     IGVTSGLGVINPLMIVAVFDNALFGPNGTCEGQPCPNLPLLYFYVGVMIAVPIVTSII
                     GIAQTYLTNDVGLNVMQDLRNRLYQHLQAMPLRFFTTTRTGEIQSRLANDVGGVQSVV
                     TDTASTVLSNVVTIVSTIIAMLVISIPLTILSLLLMPLFLWLTVKVGKARREVATNTQ
                     RTLADMTTITEETLSVSGILLSKAFGRQRFESERFRTENERLTGFLLRQTMIGRSFFA
                     LVGTFFSITPALVYLVAGWVNSSAGANVIEAGMLVGFTTLQSRLFFPIGSMLQVSTEV
                     QSSLALFDRIFEYLDLPHEIHDAPDAVAIDDVAGHVRLRDVRFRYEDPPDGVPVSPYG
                     PPLADDDPVPREWTLDGVDLDIRPGQLAALVGPSGAGKTTVTYLVPRLYDVQEGSVEI
                     DGHDVRRITLESLGEAIGVVTQETYLFHDTVRRNLQYGKPDATDEELITAAKAANIHE
                     RIAELPEGYDTVVGERGYKLSGGEKQRLAIARVVLKDPRILILDEATSSLDTTSERLV
                     QSALVPLMKGRTTIAIAHRLSTILSADVIFVLDRGRIVEQGTHDELVERNGLYAQLYE
                     QQFRGGLVQAEFEDGVILASGEVVHTES"
BASE COUNT          682 a         1495 c         1515 g          681 t
ORIGIN      
        1 cgtgggaggc ggaccgatgg gcacggccgc ggcgcgctca ctcggcgcga gggggcgtgc
       61 cgtgatcctc atcgagcgat tcacgctcgg gcacgccagg gggagcaccg cggggacgac
      121 ccgcaacttc cgcctcacgt atcacgatcc tctctacgtc cggatggcga ggcgctcgtt
      181 ggagcgatgg cgggagctgg aggacgaggc cggcgtggag ctgttgcgcg tggtgggcgg
      241 gctcgacgta ggcgcggcga ccgaggcgtc ggcgcgagcc ctcgagcacg ccggcgagcg
      301 cctcgagcgc ccgtcggacg ccgaggtcgc ggagcgctgg ccggtgctcc gcttcccgcc
      361 gcgctcgtcc ttcgtgtacc agcccgacgg ggcggtcctc cgttcccagg aggcggttcg
      421 aaccctcgcc ctattggcga gagcgcacgg tgccgaggtt cgggaggaga cggcggcggg
      481 gtcgatcgcc gcgagcggtg acggcgtcga agtggtgacg gcgtcgagca cgatccgggc
      541 cccggtggcg atcgtggcgg ccggcgcgtg gtccggcccg ctcctggggc aagcggggat
      601 ccgactgacg ctgcaaccga ccctcgagca gtcgacgtac ttcggcgcgg acgccactga
      661 cctgcccacc gtgatcgatt gggacgagac gcccgagcag cccccgtaca tcgttcccga
      721 ccccttcgag ccgggcacga tcaaggcggg cgcacatctc tccgggccgg tcgtcgatcc
      781 ggacacgagg tcgttcgatg ccgacgagac acgcgagcgt caggtcgccg attgggtggg
      841 gcggcgactc gtgtccggtg ccagacccct ccgcaccgag acgtgcctct acacgaggac
      901 gcccgacgag gacttcgtca tcgatcgggt cggaccggtc gtggtggcat ccccgtgcag
      961 cggccacggc ttcaagttca ctcccctcat cggcgaggtg ctggcggatc tcgtcatcgg
     1021 tgacgagccc gagatccctc tggaacggtt ccgcgccgat cggccggccc tccggggctg
     1081 aggccgactc acgcggtctc ctcctcggtc tcgagcagga ggtcgagcgc ctcccgcaag
     1141 ccttcgtagc gccgcccgcc cagagtcctc ctcgtccgag cttcgaccgc cgcgacggcc
     1201 ctgcggcatt cggcgccgag cgtccggccc cgtgcggtca gccggaccac cttcgcccgg
     1261 ccgtcgtccg ggtccggtgt ccggcggacg aaccctcgca cctcgagctc gtcgacgacg
     1321 accatcatcg cttgcttcgt gaccccggcc cggcgggcga gctcggtcaa ccgggtgccg
     1381 gagcgccggt cgacgttcag taccaacgaa gcctgggacg ctcggagctc gggccatcct
     1441 ctctcctcga gctccaccac gagctctgcc gcgagcgccc ggtgcgccgc gaggagctgt
     1501 cgtcccagct ggggttccgt caccgggttc atcctgccat tcccttccgg gaagtcaatg
     1561 aacctgacgg tcatcgaggg caggtcgagc acggaacgct ccgctagcct tccggcgatg
     1621 gaggtccggg aagccacgcc cgcggactac gccgaggccg gacgggtcac cgcggacgcg
     1681 tatcgcgagt acgttcggcc gggcgaggag gactgggagc gctatctcct gcggatcgcc
     1741 gacgtccggg cccgggccga ccagacgacg atcatggtgg cggtcgagga cgatcgcatc
     1801 ctcgggtcac tgaccctcga gctcgacggg cgggtgcgcg acggcgagca ggaacatcga
     1861 ccgctcgacc cgggcgaggc gcacatccgg atgctcggcg tggacccgac ggcgaggtcc
     1921 cggggcgtgg cgcgggccct gatggcggca tcggaagcgc gtgcccacga ggccggaaag
     1981 accctcatga ccttgcacac gacccagcgg atgggagccg cgcaggcgat gtacgagcgc
     2041 ctcggctacg agcgactcga ggatcgcgtg tacccggatg ggttcgtcct gctcacctac
     2101 cgcaagccgc tcgaggtttc ggggagccgt gcctgaggga atggtcctgg catgcggcaa
     2161 cggatagcga cgttccctgc catctggccc tcgcggatct ggatcgcggt gctggcgatg
     2221 accgtcctgc tggtgtcctg ccctggcggc agcggcgggt actagcgcga aagcttcaac
     2281 tctcggtgtg cacgacctcg cccgaggcga ggatcacgcc gtcctcgaac tcggcctgca
     2341 cgagaccgcc gcggaactgc tgctcgtaca gctgcgcgta gagaccgttc cgttcgacca
     2401 gctcgtcgtg ggtcccctgc tcgacgatcc gcccacgatc gagcacgaag atcacgtccg
     2461 ccgacaagat cgtcgagagt cggtgggcga tcgcgatcgt cgttcggccc ttcatcaacg
     2521 gcacgagcgc ggactgcacg agtcgctcgg aggtcgtgtc cagcgacgag gtcgcctcat
     2581 cgaggatcag gatccgcgga tccttcaaga cgacgcgggc gatcgcgagc cgctgcttct
     2641 ctcctccgga caacttgtag ccgcgctcgc cgacgacggt gtcgtacccc tcagggagct
     2701 ccgcgatccg ctcgtggatg ttcgccgcct tcgcagcagt gatgagctcc tcgtcggtcg
     2761 cgtcgggctt cccgtactgg aggttccggc ggaccgtgtc gtggaacagg tacgtctcct
     2821 gggtgaccac gccgatcgct tcgccgagcg actcgagcgt gatcctccga acgtcgtggc
     2881 cgtcgatctc gaccgagcct tcttgcacgt cgtagagccg cggcacgagg taggtcaccg
     2941 tggtcttccc cgcccccgac ggtccgacca gcgcggccag ctgcccgggc cggatgtcga
     3001 ggtccactcc gtcgagcgtc cactcccgcg gcaccggatc gtcgtcggcg agcggcggcc
     3061 cgtaggggga cacggggacg ccgtcgggtg gatcctcgta gcggaatcgc acgtcccgca
     3121 gtcgcacgtg gccggccacg tcatcgatcg ccaccgcgtc cggcgcgtcg tggatctcgt
     3181 gcgggaggtc caggtactcg aagatccgat cgaacagggc cagcgaggac tgcacctcgg
     3241 tggacacctg gagcatcgag ccgatcggga agaagagccg ggattgcaac gtggtgaacc
     3301 cgacgagcat ccctgcctcg atcacgttcg ctcccgcgct cgagttcacc cagccggcca
     3361 cgaggtagac gagcgcgggc gtgatcgaga agaaggtccc gaccagggcg aagaacgacc
     3421 ggccgatcat cgtctggcgg agcaggaagc cggtcagccg ctcgttctcg gtccggaacc
     3481 gctcgctttc gaaccgctgt cgtccgaacg ccttcgacag gaggatcccg ctgacgctca
     3541 gggtctcctc ggtgatcgtc gtcatgtccg ccagcgtccg ctgcgtgttg gtcgcgacct
     3601 cccgtcgagc cttgccgacc ttcacggtga gccagaggaa cagcggcatc agcagcagcg
     3661 agaggatcgt gagcgggatc gagatcacca gcatcgcgat gatcgtgctg acgatcgtga
     3721 cgacgttgga caggaccgtg gacgcggtgt cggtgacgac ggactgcacg ccccccacgt
     3781 cgttcgccag ccgggactgg atctcgcccg tgcgcgtcgt cgtgaagaac cgcagcggca
     3841 tcgcctggag gtgttggtac agccggttcc ggaggtcctg catcacgttc aagccgacgt
     3901 cgttcgtgag gtaggtctgg gcgatcccga tgatgctggt cacgatcggg acggcgatca
     3961 tcacgccgac gtagaagtag agcagcggca ggttcgggca gggctggccc tcgcacgtgc
     4021 cattcggacc gaagagcgcg ttgtcgaaga ccgccacgat catcagcggg ttgatcacgc
     4081 cgagccccga cgtcacgccg atcatcaacc cgaccagccc gaccttgccc ttgtacggac
     4141 ggaaagtgcc gaccacgcga cggacggtcg cacggtcgac cttccggtga tcctcgtcga
     4201 tcggagagac gcggaagaag ccgtgacgac ccatggatcg agcatacggg gggctgcgag
     4261 ctacgcccgc caccccacgt tctgtcgcgc tactccgcga agcagggacc gaagatctcg
     4321 gcgttcgtca cgtcgtcgcc ggggtcggac cagtagtgat cgaccccggc ccc
//
LOCUS       DASUVE010000253         2472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_124016, whole genome shotgun sequence.
ACCESSION   DASUVE010000253 DASUVE010000000
VERSION     DASUVE010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124016"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VF036_05515"
     CDS             complement(<1..278)
                     /locus_tag="VF036_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6264720.1"
                     /translation="MKVAMLGPIAWRTPPRHYGPWERVTSLLTEGLVRRGVDVTLFAT
                     ADSVTTATLESVAPHGYAEDADMDGRVWEALHIAHAMDLSSGFDLVHN"
     gene            448..2427
                     /locus_tag="VF036_05520"
     CDS             448..2427
                     /locus_tag="VF036_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018348678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HEX6264721.1"
                     /translation="MPDWAQFEFPASVPGPKGRRAETPLSRWLLERTVQPAGPEVREA
                     HVEQHSWWKVMCLTGVDYFSTLSYLPSIAIIAAGVVSPIATLLIVALTLLGMLPMYRR
                     VATESPHGQGSVAMLERLLPFWQGKIFVLVLLGFVATSWIITITLSSADATVHIIENP
                     LAPDLLDGQAVAITIALLLILGGVFLLGFTEAVNVAIPLVAVFLGLNAVVTGTGLVAI
                     LEHPITITDWWDALTRGGGGFSGILLPAILAFPLLVLGLSGFETGVSMMPLVAASGTT
                     PEERLASRIRNTKKLLTTAALIMCVYLITSSLVTTLLIPPEAFEPGGAANGRALSYLS
                     HEFFGDVFGTVYDISSILILWFAGASAMAGLINIVPRYLPAYGMAPDWSRAVRPVVLV
                     YTTISIIITIAFQADVDAQAGAYATGILAMMASASVAVLISAIRRRHAAPIVGFAALT
                     VILGYALVDNIVEKPDGLAISAMFILGIVAVSIISRITRTTEIRADRIEFDDTARRFI
                     EDSLVYDGRLNIIANRPGVGDAAEYDQKERAQRRLNPVPGRADVLFLEIEVVDPSDFR
                     EVLRVSGVEVDGHRVLRTGSPAAPNAIAAILIAMRDATGVLPHAYFEWSEGNPLVHMV
                     RYILLGRGDTAPVVREIVREVVKDPAERPTIHVGG"
BASE COUNT          361 a          900 c          790 g          421 t
ORIGIN      
        1 tggttgtgca cgaggtcgaa gcccgacgag aggtccatcg cgtgggcgat gtggagtgcc
       61 tcccagaccc ggccgtccat gtcggcatcc tccgcatagc cgtggggcgc caccgactcg
      121 agcgtcgccg tcgtcaccga atcggccgtc gcgaagagcg tgacgtcgac gccccggcgc
      181 accagcccct ccgtgaggag gctcgtcacc cgctcccacg gaccgtagtg ccgcggtggc
      241 gtacgccacg cgatcggtcc gagcatcgcc accttcatcg ctcgggccgc tgtgttctcg
      301 tcacgttgcg accctacgcc ccatcctcgc ggcctcctcc agccgtgcga aacgggactc
      361 cacgtggctc cggcccgagg gaacggcccg gccgactcgg tggcaggatg tgatcgtcgc
      421 cgtcgggcgg cgaggggagg cgctgtcatg cccgactggg cgcagttcga gttccccgcc
      481 agcgtcccgg ggccgaaggg tcgccgcgcc gagacacccc tctcccgctg gctcctcgag
      541 cgcaccgtcc agcccgccgg gcctgaggtc cgggaagcgc atgtcgagca gcattcctgg
      601 tggaaggtga tgtgcctcac cggcgtcgac tacttctcca cgctgtcgta cttgccgtcc
      661 atcgcgatca tcgccgcggg tgtcgtctcc ccgatcgcga ccctgctgat cgtggcgctc
      721 accctgctcg ggatgctgcc gatgtatcga cgtgtcgcca cggaaagtcc tcacggtcag
      781 ggctcggtgg cgatgctcga gcggctgctc ccgttctggc agggcaagat cttcgtcctg
      841 gtgctgctcg gcttcgtcgc aacgtcgtgg atcatcacca tcacgctgtc gtcggccgac
      901 gcgacggtcc acatcatcga gaacccgctg gcacccgacc tgctcgacgg ccaggcggtc
      961 gcgatcacga tcgcgctcct gctgatcctc ggcggtgtgt tcctgctcgg attcacggag
     1021 gccgtgaatg tggcgatccc gctcgtcgcg gtcttcctcg gcctgaacgc cgtcgtcacc
     1081 ggcaccggac tcgtcgcgat cctcgagcac ccgatcacga tcaccgactg gtgggacgcc
     1141 ctcacgaggg gtggcggcgg attctcgggc atcctgcttc ccgccatcct cgccttcccg
     1201 ctcctggtgc tggggctgtc gggcttcgag acgggggtga gcatgatgcc cctcgtcgcg
     1261 gcatccggca cgacgcccga ggagcgcctc gcgtcgcgga tccggaacac gaagaagctc
     1321 ctgacgaccg cagcgctgat catgtgcgtc tacctcatca cgagcagcct cgtcaccacg
     1381 ttgctgatcc cgccggaggc gttcgagccc ggaggcgcag ccaacggccg agcgctctcc
     1441 tatctgtcgc acgagttctt cggtgacgtg ttcggcacgg tgtacgacat cagcagcatc
     1501 ctgatcctct ggttcgccgg ggcatccgcg atggccggcc tgatcaacat cgtgccgagg
     1561 tacctgccgg cgtacggcat ggcacccgac tggagtcgcg cggtccggcc cgtggtcctc
     1621 gtctacacca cgatcagcat catcatcacg atcgcgttcc aggcggacgt cgacgcccag
     1681 gccggcgcct acgccaccgg gatcctggcg atgatggcct cggcctccgt cgccgtgctg
     1741 atctccgcga tccgacgacg ccacgcggcg ccgatcgtcg gtttcgccgc cctgaccgtg
     1801 atcctcggct acgcgctcgt cgacaacatc gtcgagaagc ccgacggcct cgcgatctcg
     1861 gccatgttca tcctcgggat cgtcgccgtg tcgatcatct cccggatcac gcgcacgacg
     1921 gagatccgcg ccgaccgcat cgagttcgac gacactgcgc ggcgattcat cgaagactcg
     1981 ctcgtctacg acggtcggct gaacatcatc gcgaatcgcc ccggcgtcgg cgacgcggcc
     2041 gagtacgacc agaaggaacg cgcgcaacgc cgactcaacc cggtccccgg ccgagcggac
     2101 gtgctcttcc tcgagatcga ggtggtcgac ccctccgact tccgggaggt cctgcgcgtc
     2161 tccggcgtcg aggtcgacgg ccaccgcgtc ctgcgcaccg gcagcccggc ggcgccgaac
     2221 gcgatcgcgg cgatcctgat cgcgatgcgg gatgccacgg gagtgctccc gcacgcgtac
     2281 ttcgaatggt cggagggcaa cccgctcgtg cacatggtgc gctacatcct gctcggacgc
     2341 ggggacaccg cccccgtcgt ccgggagatc gtccgagaag tcgtgaagga tccggccgaa
     2401 cggcccacca tccacgtcgg cggctgagtc cacgcgggag cctcccgatc cgacatccga
     2461 tcggtggatg cc
//
LOCUS       DASUVE010000254         3383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1631326, whole genome shotgun sequence.
ACCESSION   DASUVE010000254 DASUVE010000000
VERSION     DASUVE010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631326"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1409
                     /locus_tag="VF036_05525"
     CDS             24..1409
                     /locus_tag="VF036_05525"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX6264722.1"
                     /translation="MPQRRAAETATEAARRLAASGVEAIALAIVINSGVTLVKTIPLR
                     RFEEAARFGIGLSPVFTVFQVDDQITSSPHIGGPAGDTRLMPDPAQTVGLAASPGWAL
                     APVDQLDQEGEPYPGCPRTFARTMLERLAGHGLELRGGFEIEFFLGHRGAPGPDEADP
                     TPAHTGPAYGAIALADVEPFATALIGAFEAQGTGLMQFHPEYSTGQLELSVPHGVGIA
                     AADTHLVVRHTIRAVARIHGYAASFAPVVFAGRVGNGNHLHLSLWDRKGKNLFQGGRG
                     PESMTRGAEAFTAGVLEALPALTAITAPSVPSYERLQPHRWSGPWACWGRENREAALR
                     FVTGMVGSRPDAANLEVKSMDPAANPYLALGAIVAAGIDGLDRELSLPEPVGDDPASI
                     PARRRRSMGIRRLPDSLSAAIRELEGSSMLREAMGDVLFDSFLATRRAERDVFEGQDP
                     EDVVRAHRWRY"
     gene            1409..2584
                     /locus_tag="VF036_05530"
     CDS             1409..2584
                     /locus_tag="VF036_05530"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6264723.1"
                     /translation="MPDVDLSGLDIVDAHSHPYRLDDLRSKGSTGFDTRMMFLGESFL
                     SSSQMKDELWPVADSFTDSTVFALAMRRWLADHLGCEPTRAAVTEAREAAIRQDAVAY
                     TKGLLDAAGVVAVLSDEGYPQPPISAAEFAETIGVTVHRVARLEPWILARRDGSFDDL
                     VAGVEADATEAASDPNCVAYKSIVAYRTGLDVGDPSASDAEAAFDRWRHDGWAETREH
                     AKPVRDFLIRRALAIAKTNDRPFHFHCGGGDPDINLRHADPISIFPLLVDVQDQPVVL
                     VHSGYPWVREAAYVASVLPNVYLELSELIPWGWGQVEWALEMLVGTVPAGKLLYGSDE
                     AGEPEAFWASGLLARSALRRVLGGFVDRDYVTADEAEDLGRLVLGDACRRLHGVRAD"
     gene            complement(2634..>3383)
                     /locus_tag="VF036_05535"
     CDS             complement(2634..>3383)
                     /locus_tag="VF036_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264724.1"
                     /translation="LWATWGNMVVIEAGGANGTTGGAETVGLSLGRWTGAAVALVMAW
                     VVLTVAVVYNFSFGRLLFVSGMEKRLPHQFGKVNRNQVPANAITLQSAISTILAVVIF
                     FVLGSGETDPYRYFYGLYAGVTIVWCISTALLFLDIFFAKRAEPERFERERRVPTGFL
                     FLCGVIGFVVNILAVLFIFVGSWYPVSDSNPTGWELADWNVFMVVFTLVSVVSGIVIW
                     FVSQSARRGKSDEELIAEGLADREIVEGSSA"
BASE COUNT          533 a         1220 c         1143 g          487 t
ORIGIN      
        1 ggtatggacg taagaggtac gtgatgccgc agcgacgcgc cgccgagacg gccacggagg
       61 ccgcgcgacg cctcgccgcc tccggcgtcg aggcgatcgc gctcgccatc gtgatcaact
      121 cgggcgtcac cctcgtcaag acgatcccgc tccgtcggtt cgaggaggcg gctcggttcg
      181 ggatcggcct ctcccccgtc ttcacggtct tccaggtcga cgatcagatc acgtcgtcgc
      241 cgcacatcgg tggaccagcg ggcgacacgc ggctgatgcc ggatccggcg cagacggtcg
      301 ggctcgccgc gagcccgggg tgggcgctcg cgcccgtgga ccagctcgac caggagggcg
      361 agccgtaccc gggctgtccg cgcacgttcg cgcggacgat gctcgagcgg ctcgccggcc
      421 acgggttgga gctccgcggg ggattcgaga tcgagttctt cctcgggcat cgcggcgcgc
      481 cgggaccgga cgaggccgat ccgacgccgg cccacacggg gcccgcctac ggagcgatcg
      541 cgctcgcgga cgtcgaaccg ttcgcgacgg cgctgatcgg cgccttcgag gcgcagggaa
      601 cgggcctcat gcaattccac cccgagtact cgaccgggca gctcgaactg tcggtgccgc
      661 acggcgtcgg gatcgccgcc gcggacaccc acctcgtcgt ccgccacacg atcagggcgg
      721 tggcgcgcat ccatggctac gccgcctcgt tcgcgccggt cgtcttcgcg gggcgcgtcg
      781 gcaacgggaa ccacctgcac ctgagcctgt gggaccggaa ggggaagaac ctgttccagg
      841 gagggcgcgg tcccgagagc atgacgcgcg gagcggaagc gttcacggcg ggggtgctcg
      901 aggcactccc cgcgctcacc gcgatcaccg caccgtcggt tccgagctac gaacggctcc
      961 aaccccaccg ctggagcggt ccgtgggcct gctgggggcg ggagaaccgc gaggcggcgc
     1021 tccggttcgt gaccgggatg gtcgggagcc gaccggatgc cgcgaacctc gaggtcaagt
     1081 ccatggatcc ggcggcgaac ccctacctcg ccctcggcgc gatcgtcgcc gcggggatcg
     1141 acggcctcga tcgcgagctc tcgctccccg agcccgtcgg cgacgatccg gcctcgatcc
     1201 cggctcgccg tcgccgctcg atggggatcc gccggctgcc cgactcgctc agcgccgcga
     1261 tccgcgagct cgaggggtcc tcgatgctcc gcgaagcgat gggcgatgtc ctgttcgact
     1321 cgttcctcgc gaccaggcgc gcggaacgcg acgtcttcga aggccaagat cccgaggacg
     1381 tcgtccgcgc tcaccgatgg aggtactgat gcccgacgtc gatctgtccg gactcgacat
     1441 cgtcgacgcg cactcgcacc cgtaccggct cgacgacctc cggagcaagg gctcgaccgg
     1501 gttcgacacg aggatgatgt tcctcggcga atccttcctc tcttcgtcac agatgaagga
     1561 cgagctgtgg ccggtggcgg actccttcac ggactccacg gtgttcgccc tcgcgatgcg
     1621 ccgatggctc gcggatcacc tcgggtgcga gccgacccgc gcggccgtca ccgaggcacg
     1681 ggaggcggcg atccggcagg acgccgtcgc gtacacgaag ggactgctcg acgccgccgg
     1741 cgtcgtcgcg gtcctgtcgg acgagggcta cccgcagccg ccgatctccg cggcggagtt
     1801 cgcggagacg atcggcgtga ccgtccaccg ggtcgccagg ctcgagccct ggatcctcgc
     1861 acggcgggac ggctcgttcg acgacctcgt cgccggcgtg gaggccgacg cgacggaggc
     1921 ggcatcggat ccgaactgcg tggcctacaa gtcgatcgtc gcgtaccgca ccggcctcga
     1981 cgtcggcgac ccctccgcgt cggacgccga ggcggccttc gaccgctggc gccacgacgg
     2041 gtgggccgag acccgcgaac acgcgaaacc cgtccgggac ttcctgatcc ggcgcgccct
     2101 cgcgatcgcg aagacgaacg accggccgtt ccacttccac tgcggcggcg gggaccccga
     2161 catcaacctc cgtcacgccg acccgatctc gatcttccct ctcctcgtcg acgtgcagga
     2221 tcagccggtc gtcctcgtgc actcggggta cccgtgggtc cgggaagccg cctacgtcgc
     2281 atcggtcctc ccgaacgtgt acctggaact ctccgagctg atcccgtggg gatggggcca
     2341 ggtggagtgg gccctggaga tgctcgtcgg gaccgtgccg gcgggcaagc tcctgtacgg
     2401 ctccgacgag gccggcgagc ccgaggcgtt ctgggcgtcg ggcctactcg ctcgatccgc
     2461 gctgcggcgg gtgctcggag gcttcgtcga tcgcgactac gtgaccgcgg acgaagcgga
     2521 agacctgggg agactcgtgc tcggcgacgc gtgccgccgc ctccacggcg tcagggccga
     2581 ctgaatggtc cggccgccgc cctcgcgcgg gcggcggccg acgtcccgaa gtcctaggcc
     2641 gaggaccctt cgacgatctc gcgatcggcg agtccctcgg cgatcagttc ctcgtccgac
     2701 ttgccgcgtc tcgccgactg cgacacgaac cagatcacga tgcccgagac caccgacacg
     2761 agcgtgaaca cgaccatgaa gacgttccaa tccgcgagct cccatcccgt cgggttcgaa
     2821 tccgacaccg ggtaccacga gcccacgaag atgaagagga ccgcgaggat gttgaccacg
     2881 aatccgatca caccgcagag gaacaggaac ccggtcggga cccgccgctc gcgctcgaac
     2941 cgctccggct ccgctcgctt cgcgaagaag atgtcgagga acagcaaggc ggtcgagata
     3001 caccacacga tcgtgacgcc ggcgtagagc ccgtagaagt atcggtaggg gtccgtttcg
     3061 cccgatccga gcacgaagaa gatcacgacc gcgaggatcg tcgagatcgc gctctggagc
     3121 gtgatcgcgt tcgccggcac ctggttccgg ttgaccttcc cgaactgatg cgggaggcgc
     3181 ttctccatcc ccgacacgaa cagcagtctc ccgaaggaga agttgtagac gacggcaacg
     3241 gtcaacacga cccacgccat caccagtgcc acggccgcgc ccgtccacct cccaagcgac
     3301 aggcccaccg tctccgcacc tcccgtggtc ccgttcgcgc ccccggcctc gatcacgacc
     3361 atgttccccc aggtggccca gag
//
LOCUS       DASUVE010000255         3001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_313406, whole genome shotgun sequence.
ACCESSION   DASUVE010000255 DASUVE010000000
VERSION     DASUVE010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313406"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /gene="tig"
                     /locus_tag="VF036_05540"
     CDS             complement(<1..1285)
                     /gene="tig"
                     /locus_tag="VF036_05540"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX6264725.1"
                     /translation="METTVETVEPHTVKLTIEVPPEEFRKDLDRTYRAIANEIKVPGF
                     RKGKVPKQIIDTQIGRDAIFEQFVNTSVPTYFRQAVTDEDLAPITDPDIDVQQLEPGR
                     PFLFTATVEVRPRLTFEEDDYTGISVTKPPIDVSEEEIDDWIERLRERFAELEPVGRP
                     VQQGDFVTVDLTVMKGSEKVEQASRADYLYTVGSGEVGEKLDVELVGTKPGAILKVAD
                     SLPERFGEELGGAPVDISVIVKDVKARRLPEVDDQFAKTASEFDTIPQLRDDLRERLT
                     EVKEREAIAALRDLVLQAMIEKVDVDRPDSLIEEETDHRIHHARDQAERMGIGLEKML
                     ELQGWDEARLREDSRQHAIRAIKSDLVLEGIARSASLDVTAEEIGAEIAVLAQAYGRE
                     PKAVAKDLERSGQVVTLAGDIIRSKALDLLVERADV"
     gene            1312..2139
                     /locus_tag="VF036_05545"
     CDS             1312..2139
                     /locus_tag="VF036_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX6264726.1"
                     /translation="MTLGSRRHRFDVGRAPGRDWYGRAVFEKELAFAHEIADRAAEIS
                     MSYFLGEFEVRQKPDLSPVTQADLEVETAVREMIAARFPTDAITGEEHGEGAGDRVWI
                     VDPIDGTRNFADGVPIWATLLALQVDGRSVLGMASAPALGERYEAVRGGGARWNGREL
                     HASERKLDDAFLAFSSVDDWLGGPREDAFVELVRDTRRSRGFGDFWGHMLVARGAVDL
                     MIEPSLRIWDWASIVVIVEEAGGVVTTFEGEEPSDGASILTSNAVVHDEVVRRLSGP"
     gene            2136..2726
                     /locus_tag="VF036_05550"
     CDS             2136..2726
                     /locus_tag="VF036_05550"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX6264727.1"
                     /translation="MKRCAWAPVDNASYLAYHDEEWGVPVHDDQRLFEMLTLEGAQAG
                     LSWATILNKREGYRRAFADFDPSIVARFSRRKVERLLADPSIVRNRMKVESTINNARR
                     VLSVQEEDGSLDAYLWSFVGGSPKVNRFRSLGDIPPETPESKAISKDLKRRGFRFVGP
                     TVCYAFMQACGLVMDHVASCFRYSELATLADKPAGR"
BASE COUNT          438 a         1014 c         1025 g          524 t
ORIGIN      
        1 tcacatcggc acgttcgacc aggaggtcga gggccttgct cctgatgata tccccggcca
       61 gcgttacgac ctgaccgctg cgctcgagat ccttggcgac ggctttcggc tcccggccgt
      121 aggcctgcgc gaggacggcg atctccgcgc cgatctcctc ggccgtgacg tccagggatg
      181 cgctgcgagc gatgccttcc aggacgaggt cgctcttgat cgctcggatc gcgtgctggc
      241 gggagtcctc gcgcagacgc gcctcgtccc atccctgcag ctcgagcatc ttctcgaggc
      301 cgatccccat ccgctccgcc tggtcgcgcg cgtggtggat ccggtggtcg gtctcctcct
      361 cgatcaggct gtcgggtcgg tcgacgtcga ccttctcgat catcgcctgc agcacgagat
      421 cgcggagcgc cgcgatggcc tcgcgctcct tcacctcggt gagccgctca cgcaggtcgt
      481 cgcgcagctg ggggatcgtg tcgaactccg aggccgtctt tgcgaactgg tcgtccacct
      541 cggggaggcg ccgggccttc acgtccttca cgatcacgct gatatcgacc ggcgcgccac
      601 ccagctcctc gccgaaacgc tccggcagcg agtcggccac tttcaggatc gcgccgggct
      661 tcgtgcccac gagctccacg tcgagcttct cccccacctc gccggagccc acggtataga
      721 ggtagtccgc gcgcgaggcc tgttcgacct tctcgctccc cttcatcacg gtgaggtcca
      781 cggtcacgaa gtcgccctgc tgaacgggcc tcccgaccgg ctcgagctcc gcgaaccgct
      841 cccgcaggcg ctcgatccag tcgtcgatct cttcttcgct cacgtcgatc ggcggcttcg
      901 tgacgctgat ccccgtgtag tcgtcctcct cgaaggtgag gcggggacgg acctccacgg
      961 tggcggtgaa caggaagggc cgacccggct ccagctgctg cacgtcgatg tcgggatcgg
     1021 tgatcggcgc gagatcctcg tcggtgacgg cctgtcggaa gtaggtgggg acggacgtgt
     1081 tcacgaactg ctcgaagatc gcgtcccggc cgatctgggt gtcgatgatc tgcttcggca
     1141 ccttgccctt ccggaacccg gggaccttga tctcgttggc gatcgctcgg taggtgcggt
     1201 cgaggtcctt gcggaactcc tcgggaggca cctcgatcgt gagcttcacg gtgtgcggtt
     1261 cgacggtttc gacggtcgtt tccatggagg acccaggcta ctcgacccgg ggtgacactc
     1321 ggctcgcggc ggcatcgctt cgacgtcgga agagcgcccg ggcgcgactg gtacggtcgg
     1381 gccgtgttcg agaaggagct cgccttcgcg cacgagatcg ccgatcgggc ggcggagatc
     1441 tcgatgtcgt atttcctcgg ggaattcgag gtcaggcaga agccggacct ttcgcccgtg
     1501 acccaggcgg acctggaggt ggagaccgcc gtccgagaga tgatcgcggc gcggttcccg
     1561 accgacgcga tcaccgggga ggagcacggg gagggcgccg gcgatcgcgt gtggatcgtc
     1621 gatccgatcg acggcaccag gaacttcgcg gacggggtcc cgatctgggc aacgctgctc
     1681 gccctccagg tggacggccg atcggtgctc ggcatggcga gcgcgcccgc gctgggcgaa
     1741 cgctacgagg ccgtccgcgg gggcggcgcc cggtggaacg gccgggagct ccacgcgagc
     1801 gagcggaagc tcgacgacgc gttcctcgcg ttctcgagcg tcgacgattg gctcggcggc
     1861 cctcgcgagg acgccttcgt cgaactcgtc cgcgacacgc ggcgcagccg cgggttcggt
     1921 gacttctggg ggcacatgct ggtggcgcgt ggcgccgtgg acctgatgat cgagccgtcc
     1981 ctacggatct gggactgggc ctcgatcgtc gtgatcgtcg aggaagccgg cggcgtcgtc
     2041 accacgttcg agggtgagga gccgtcggac ggcgcgagca tcctcacgtc caacgcggtc
     2101 gtgcacgacg aggtcgtccg acgcctctca ggcccgtgaa gcgctgcgcg tgggcgcccg
     2161 tggacaacgc ctcctacctc gcgtaccacg acgaggaatg gggcgtcccg gtccacgacg
     2221 accagcggct cttcgagatg ctgacgctcg agggcgccca ggccggcctg tcgtgggcaa
     2281 cgatcctgaa caagcgcgag ggctaccgtc gcgcgttcgc tgacttcgac ccgagcatcg
     2341 tggcgcgctt cagcaggcgg aaggtcgagc ggcttctcgc ggatccctca atcgtccgca
     2401 accgcatgaa ggtggagtcg acgatcaaca acgcccgacg ggtgctctcc gtccaggagg
     2461 aggacggttc gctcgacgcg tacctctggt ccttcgtcgg cggctcgccg aaggtgaacc
     2521 ggttccgcag cctcggggac atccccccgg agactccaga gtcgaaagcc atctcgaagg
     2581 atctgaagcg gcgtgggttc cgtttcgtcg ggccgaccgt gtgctacgcg ttcatgcagg
     2641 catgcggcct ggtcatggac cacgtagcga gttgcttccg ctattccgag ctcgcaactc
     2701 tcgcggacaa gcctgctggc cggtgaaggt cagcagctcg gagacccaag cctgggttct
     2761 cccggacatg cagccgcccg atcagcgcgg ccgggtctgc agcccaccgc tccaggatct
     2821 cgagcagctc gcgggcagcc ggctcgccga gggcggcgac cacccgctgg ccggcgtcgt
     2881 tcagctcacc aaagcctcga gtcggacgat gccgcccgcg tggaggatct ggtccatgat
     2941 ctccgtcatg cctgcggcgc ggaggttccg acacaccgtg atgccctcga ctgagcggct
     3001 g
//
LOCUS       DASUVE010000256         5315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1443791, whole genome shotgun sequence.
ACCESSION   DASUVE010000256 DASUVE010000000
VERSION     DASUVE010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443791"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VF036_05555"
     CDS             <1..814
                     /locus_tag="VF036_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX6264728.1"
                     /translation="TDIWLPYYRDLGIVLSAGMTPYEVFLGVFAKAGDPSSGGRQMPA
                     HWGSRRLGIISGSSPIATQVPHAAGIAYAARYRGEDAVVGCWFGDGATSEGDWHEGLN
                     FSGIHRLPVIFVCENNQYAISVPQSKQMAVKDVASRAEGYGFPGVVVDGNDVLACYGA
                     MKIAHERARSGEGPTLIECKTYRFMPHTSDDDDKSYRSRDEVEQARARDPLVQFASYL
                     REHGLLDDAAFDALHAEIKEEIDEAIRAAWDAPDPDPDTALRHVFQEREEEP"
     gene            811..1800
                     /locus_tag="VF036_05560"
     CDS             811..1800
                     /locus_tag="VF036_05560"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX6264729.1"
                     /translation="MTEKNVVTAVRDALHDEMANDERIVLLGEDVGARGGVFRISDGW
                     MEEFGEERVIDTPLAESGIIGIAIGMALHGLLPVAEIQFADFIHPAFDQVVSEAARLR
                     YRSNGDFGVPMVIRTPYGGGIHGALYHSQSIEAFYAHIPGIKVVVPATPADAAGLLRS
                     AIQDPDPVLFLEHKKTYRSVKGEVADGAYLTPIGIAEVKRDGNDLTCISYGYSLHLCL
                     DAAQRLAEDEGVSVEVVDVRTLAPLDVDTVVGSVRKTGKAMVVYEDNRTYGAGAEIAS
                     TIAEQALFDLDAPILRIGGPDIPAMPFAAPLEHYFMEPEPDHIYARMRELARA"
     gene            1828..2805
                     /gene="lipA"
                     /locus_tag="VF036_05565"
     CDS             1828..2805
                     /gene="lipA"
                     /locus_tag="VF036_05565"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX6264730.1"
                     /translation="MTSTAPGTDHNPNKRRLHLPIVGVTNGPGVEAIAGRKPPWLKVR
                     LTTGPNYEDLKGIMRERSLHTVCEEAMCPNIRECWEEREATFLILGSKCTRRCGFCDV
                     MTAKPNPVDEGEPVRIAEAVGEMGLRYVVLTGVARDDLEDGGARIWASAIRAVRAAVP
                     GIGVEVLPSDFKGGERDIATVLGAEPDVFAHNLETVRRLHDRIRPAFGYDRSLDVLRF
                     AKRYRAGQVTKSNLILGMGERPEEIPTAMTDLRDAGIDILTMGQYLQPTGYHLPVDRW
                     VHPDEFAEWKRIGEEMSIAHVEAGPLVRSSYHAGKQLRRAVEAGVSAPA"
     gene            complement(2791..3099)
                     /locus_tag="VF036_05570"
     CDS             complement(2791..3099)
                     /locus_tag="VF036_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264731.1"
                     /translation="MAHWPRTATGKAFYVALIAVVVPLTVWFLVTVPSYVLWALGLIA
                     IISVALVIWRRRLDAAREKAWVGEFSFGDVVGRMKARDALDRAREPGALTAASASSGG
                     "
     gene            complement(3509..4486)
                     /locus_tag="VF036_05575"
     CDS             complement(3509..4486)
                     /locus_tag="VF036_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2578c family radical SAM protein"
                     /protein_id="HEX6264732.1"
                     /translation="MDQLFEIARTTFDTPEFRGITFIESEAKSIINRVPGGALPFDWT
                     INAYRGCSHACVYCFARPTHTYLDMNAGRDFETKIVVKVNAPDVLRRELAAKRWKGEH
                     IAMGTNTDPYQRAEGRYKLMRGILEALVDYRNPFSILTKGTLILRDVDLLQRAAEVTT
                     VSAAFSIGTLDEAVWRETEPGTPHPKARIDAIRTLTEAGIPTGVLMAPILPGISDHPQ
                     QLRELVEAAIDAGATHVSPILLHLRPGVREEFLPWLEEVHPDLVPRYLEMYRQPYGPT
                     ALREGLGRRVSSMIAAAGGIRRSVEPARRFFRRDRERPRERANEQLRLL"
     gene            complement(5063..>5315)
                     /gene="rnpB"
                     /locus_tag="VF036_05580"
     ncRNA           complement(5063..>5315)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF036_05580"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          867 a         1801 c         1831 g          816 t
ORIGIN      
        1 caccgatatc tggctcccct actaccgaga cctcggcatc gtcctcagcg ccgggatgac
       61 accgtacgag gtcttcctcg gcgtcttcgc caaggccggc gatccgtcct cgggcggccg
      121 gcagatgccg gcgcactggg gctcccgacg cctcggcatc atcagcggct cgtctccgat
      181 cgccacgcag gtcccgcacg cggccgggat cgcctacgcc gcgaggtacc ggggggaaga
      241 cgccgtcgtc ggctgctggt tcggcgacgg cgccacgagc gaaggcgact ggcacgaggg
      301 cctgaacttc agcgggatcc accgtttgcc ggtgatcttc gtgtgcgaga acaaccagta
      361 cgcgatcagc gtcccgcagt ccaagcagat ggccgtgaag gacgtggcga gtcgtgccga
      421 ggggtacggc ttccccggcg tcgtcgtcga cggcaacgac gtcctcgcgt gctacggcgc
      481 gatgaagatc gcacacgagc gcgcccgatc cggtgagggt cccacgctga tcgagtgcaa
      541 gacgtaccga ttcatgcccc atacctccga cgacgacgac aagagctacc gctcgcgcga
      601 cgaggtggag caggcgaggg cccgcgaccc gctcgtgcag ttcgcctcgt acctgcgcga
      661 gcacggtctg ttggacgatg ccgcgttcga cgcgctccac gcggagatca aggaggagat
      721 cgacgaggcg atccgggccg cgtgggacgc gccggatccg gacccggaca ccgccctccg
      781 tcatgtcttc caagaacgag aggaggagcc gtgacggaga agaacgtggt caccgcggtg
      841 cgcgacgcgc tccacgacga gatggcgaac gacgagcgca tcgtgctgct cggcgaggac
      901 gtgggcgcgc gcggcggcgt cttccggatc tccgacggct ggatggagga gttcggcgag
      961 gagcgggtga tcgacacgcc gttggcggaa tcggggatca tcgggatcgc gatcgggatg
     1021 gcgctgcacg gcctgttgcc cgtcgcggag atccagttcg cggacttcat ccatccggcg
     1081 ttcgaccagg tcgtctccga ggccgccaga ctccggtacc gctcgaacgg cgacttcggc
     1141 gtcccgatgg tgatccgtac gccgtacggg ggcgggatcc acggggccct gtaccactcg
     1201 cagtcgatcg aggcgttcta cgcccacatc cccgggatca aggtcgtcgt cccggccaca
     1261 ccggccgacg ccgcaggtct gctccggagc gcgatccagg accccgatcc ggtcctgttc
     1321 ctcgagcaca agaagaccta ccgctcggtc aagggggagg tggccgatgg cgcgtacctc
     1381 acgccgatcg gcatcgcgga ggtgaagcgg gacggcaacg acctgacgtg catcagctac
     1441 ggctactcgc tccatctgtg cctggacgcg gcgcagcgtc tcgccgagga cgagggcgtc
     1501 tcggtcgagg tcgtggacgt gcgcaccctc gcgccgctcg acgtcgacac cgtcgtgggc
     1561 tccgtccgca agaccgggaa ggcgatggtg gtctacgagg acaaccggac ctacggcgcc
     1621 ggcgcggaga tcgcctccac gatcgccgaa caggccctct tcgacctcga cgcgccgatc
     1681 ctgcgcatcg gcgggccgga catcccggcg atgccgttcg cggcgccgct cgagcactac
     1741 ttcatggagc ccgagccgga tcacatctac gcgcggatgc gcgagctcgc ccgcgcatag
     1801 cgggagggct ggctacactc ggaagggatg acatccacgg cgcccgggac cgaccacaat
     1861 ccgaacaagc ggcgcctcca tctcccgatc gttggcgtca cgaacgggcc gggtgtcgag
     1921 gccatcgccg ggcgaaagcc cccgtggctc aaggtgcgcc tcacgacggg tcccaactac
     1981 gaggacctca agggcatcat gcgcgagcgt tcgctgcaca cggtgtgcga ggaggcgatg
     2041 tgcccgaaca tccgcgagtg ttgggaggaa cgcgaggcga cgttcctgat cctggggtcg
     2101 aagtgcacac gccgctgtgg cttctgcgac gtgatgacgg cgaaaccgaa cccggtggac
     2161 gaaggggagc cggtcaggat cgccgaggcc gtcggcgaga tggggctgcg gtacgtcgtg
     2221 ctcaccggcg tcgcccgcga cgacctcgag gacggagggg cgcgcatctg ggcctcggcg
     2281 atccgagcgg tccgcgcagc ggtaccgggg atcggcgtgg aggtcttgcc gtcggacttc
     2341 aagggcggcg agcgcgacat cgcgacggtg ctcggggccg agccggacgt gttcgcccac
     2401 aacctcgaga ccgtgcgtag gctccacgac cggatcaggc cggcgttcgg ctacgaccga
     2461 tccctggacg tgctgcgctt cgccaagcga taccgcgccg gccaggtcac gaagtcgaac
     2521 ctgatcctcg ggatgggcga gcggcccgag gagatcccga ccgcgatgac ggatctccgc
     2581 gacgccggca tcgacatcct gacgatgggt cagtacctgc agccgaccgg gtaccacctc
     2641 cccgtcgatc gatgggtgca ccccgacgag ttcgctgagt ggaagcggat cggcgaagag
     2701 atgagcatcg cccacgtcga ggcagggccg ttggtccgct ccagctacca cgccggaaag
     2761 cagctccggc gcgcggtgga agccggggtc tcagcccccg cttgacgcgg aggcggccgt
     2821 cagcgcgccg ggttcccggg cgcgatcgag cgcgtccctc gccttcatgc gaccgaccac
     2881 gtcgccgaac gagaactcac cgacccaggc cttctcgcga gccgcgtcga gtctgcgccg
     2941 ccagatcacg agagcgaccg agatgatcgc gatcagaccg agcgcccaga gcacatagct
     3001 gggaaccgtc accaggaacc agacggtgag cggtacgacg actgcgatca acgcgacgta
     3061 gaacgccttg cctgttgcgg tccttggcca gtgggccatg tcaacctccc gatctcgaag
     3121 ggaaccgtgg aaggcaaggt cgcgaggtct tcgcgcgttc gccccgtctt gggcggttcc
     3181 accagtggtg gacgtgctct ctgtcagtct ccgagggtgc ttcggtcccc ggcggtgcga
     3241 tcccgcagga acgatcctct tcctcggtcc gagtggagcg tgaacccatt cgggacccgt
     3301 ggggacgcgt tccgcgtccg gggtctcagc cgatgggaca gggattccag cagtcgagcc
     3361 gtgggatcgc gcaaggtggt cctcattcgg tgccctccct cattcggggg gctgccctcg
     3421 gcctcttcgg gagcggtccc gctcacttcg tatgtctatc ctctcacttc cggccttcgg
     3481 cacgacgggt cacgccgacc caccaacgct agagcagacg gagctgctcg ttcgcgcgtt
     3541 cgcgcggccg ctcgcggtcc cgtcggaaga accgacgcgc cggctcgacg ctccgacgga
     3601 tcccgccggc ggcggcgatc atcgacgaca cccggcgacc gagaccctcg cggagggccg
     3661 tcggtccgta cggctgccgg tacatctcga ggtagcgcgg gacgaggtcg gggtggactt
     3721 cctcgagcca tgggaggaat tcctcgcgga cgccggggcg caggtgcaac aggatcggcg
     3781 agacatgggt cgcccccgcg tcgatcgcag cctcgacgag ctcgcggagc tgctgggggt
     3841 gatccgagat gcccggcagg atcggcgcca tcaggacgcc ggtggggatg cccgcctcgg
     3901 tgagcgtccg gatcgcgtcg atccgcgcct tcggatgggg cgtgccgggc tcggtctcac
     3961 gccagaccgc ttcgtccagc gtgccgatcg agaacgccgc ggagaccgtg gtgacctcag
     4021 ccgctcgctg cagcaggtcg acgtcgcgaa ggatcagcgt gcccttcgtc aggatcgaga
     4081 acgggttccg gtagtcgacc agcgcctcga ggatcccccg catcagcttg taccggccct
     4141 ccgcgcgctg atacgggtcg gtgttcgtgc ccatcgcgat gtgctcgccc ttccatcgct
     4201 tcgcggccag ctcgcgacgc aacacgtcgg gggcgttcac cttcacgacg atcttcgtct
     4261 cgaagtcacg gccggcgttc atgtcgagat acgtgtgcgt cggcctcgcg aagcagtaca
     4321 cgcaagcatg gctgcatcct cgatacgcgt tgatcgtcca gtcgaacggc agggcgccgc
     4381 ccggcacgcg gttgatgatc gacttcgcct cggattcgat gaacgtgatg ccgcggaact
     4441 cgggcgtgtc gaaggtcgtc cgggcgatct cgaagagctg atccatggac ctgacaggat
     4501 agaacgtatg ttcgattctg cctaggcccg ctcagtgggg agcatcgacc cgctcggcga
     4561 gcctcggcag catcgccagg gcgatctcag gctcctcctt gagcagttcc tggaagtcgc
     4621 tcctggagac gcgaggcacc gcaccgacgt cttcgcccgg acgcgagcct tccgcggatc
     4681 atcggtgagg agcgcgagct tccgaagaac tcgcccactc cgagctccca ggaccggccg
     4741 tcgggggtct gggcctccac ggtcccctcc tcgaggacga acatgcccga gccagggctg
     4801 acccacctcg acgaggaccc atccggcggg ctcctcgaac tcggtcgaga gctcggcgat
     4861 ccgctccaac gttcggtcgt gcaggtccgc gaacagcggg atccgtcgga gcttctcgat
     4921 gtccatcgcg aggacgctac acgaccgatg agaaggggtt cggggctcaa gcagtgaccg
     4981 acgaaggagc gaacgcagcc ccgatggatg cattggggct caagcagtga gcgacgaacg
     5041 agcgaacgta gccccgagaa actggaggag ccggcctgta agccggatcc tgtgcccggg
     5101 accgaggtcc cggcggcggc catctctctc ggcgcgcagg cgggtctccc cgccgcggcg
     5161 ccgcgtcgcg gttcacccgc gccgtgcggc ctacccggca gctcgggcgg gccgccctcg
     5221 aacgctgcct gcttggcctt gctccgggtg gggcttgccg agccggcgcg gtcacccgcg
     5281 ccgctggtgc gctcttaccg caccgtttca ccctt
//
LOCUS       DASUVE010000257         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_313656, whole genome shotgun sequence.
ACCESSION   DASUVE010000257 DASUVE010000000
VERSION     DASUVE010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313656"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VF036_05585"
     CDS             complement(<1..577)
                     /locus_tag="VF036_05585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383626.1"
                     /note="involved in translesion DNA polymerization with
                     beta clamp of polymerase III; belongs to Y family of
                     polymerases; does not contain proofreading function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX6264733.1"
                     /translation="MFVSSQPTILHADVDSFYASVEQRDDPSLRGRPVIVGAGVVLAA
                     SYEAKAFGITTPMGGAQARRLCPHAIVVPPRMDAYSEASTALFEVFNDTTPFVEGLSI
                     DEAFLDVGGLRRISGTPMEIAVRLRREVLERVGLPVTVGVARTKFLAKVASGVAKPDG
                     LLVVPPDGELAVLHPLPVERLWGVGPVTARKL"
     gene            complement(615..1196)
                     /locus_tag="VF036_05590"
     CDS             complement(615..1196)
                     /locus_tag="VF036_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006107454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6264734.1"
                     /translation="MSKVTADISMSLDGFVTEPDPGGDNPLGDDPGRLHDWMFSAKTA
                     TDAEIVDEIYATTGAVLIGKRMFDVGVEPWGDPPPFGMAVFVVTHETRDPLPRRGGTT
                     YHFVTRGLDAALAVARAAADDKDVGVWGGPNIVTQYLQAGLLDELQLHVVPILLGNGV
                     RLFQDLNAPRIELRKTRSIDTPRATHLRFSVLS"
     gene            complement(1213..1284)
                     /locus_tag="VF036_05595"
     tRNA            complement(1213..1284)
                     /locus_tag="VF036_05595"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1249..1251),aa:Arg,seq:ccg)
     gene            1404..1769
                     /locus_tag="VF036_05600"
     CDS             1404..1769
                     /locus_tag="VF036_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6264735.1"
                     /translation="MPRVLFADDDPILHRLLEVNFRVVGITIESVGRGDAALERVVAA
                     PPDALVLDATMPGMDGHEVYRRLREVPALAELPVIFLTGRGAEEFEAYAGPRVRIMTK
                     PFDPTELVQVVREAMGEGG"
     gene            1766..>2041
                     /gene="argS"
                     /locus_tag="VF036_05605"
     CDS             1766..>2041
                     /gene="argS"
                     /locus_tag="VF036_05605"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.4"
                     /note="catalyzes a two-step reaction, first charging an
                     arginine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA
                     synthetase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX6264736.1"
                     /translation="MIEERLRNLLADAIAKAAPSLGAATPLPVPELQRPRQKGFGDFT
                     TNVAMVIASHVGADPRATAAAIVEAMPSATFLAKAEVAGPGFINLWTT"
BASE COUNT          328 a          706 c          699 g          308 t
ORIGIN      
        1 ggagcttgcg agcggtgacc gggccgacac cccagagccg ctccaccggg agcgggtgga
       61 ggaccgccag ctcgccgtcg ggtggaacaa ccaacagacc gtcgggcttg gccacgccgc
      121 tcgccacctt ggccaggaac ttcgtcctcg cgacacccac ggtgacgggc aggccgaccc
      181 gctcgaggac ctctcgccgc agtcggacgg cgatctccat cggcgtacca gagatccgcc
      241 gcaacccgcc gacatcgagg aacgcctcgt cgatcgacag cccttcgacg aacggcgtcg
      301 tgtcgttgaa cacctcgaac agggccgtgc tcgcctcgct gtaggcatcc atccgggggg
      361 gaacgacgat cgcgtgcggg cagagccttc gcgcctgggc tccgcccatc ggtgtggtga
      421 tgccgaacgc cttcgcctcg tagctggccg ccagcaccac gccggccccg acgatgacgg
      481 gacggccgcg caagctgggg tcgtctcgct gctcgaccga cgcgtagaac gagtcgacgt
      541 ccgcgtgcag gatcgtcggt tggctcgaca cgaacacatg ttcgcatctc gcggtgacgc
      601 cggaccaacg acgctcagga gaggacggag aaccggaggt gggtcgccct cggggtatcg
      661 atcgacctcg tcttcctcag ttcgatcctc ggggcgttca ggtcctggaa gaggcgcacg
      721 ccgttcccca gcaggatcgg gaccacatgg agctgcagct cgtccaagag ccctgcctgg
      781 aggtactgcg tgacgatgtt cggtcctccc cagacgccga cgtccttgtc gtccgcggcg
      841 gcgcgggcga cggccagcgc agcgtccagt ccgcgggtca cgaagtggta cgtcgtgcca
      901 ccccgtctcg gcagagggtc acgtgtctcg tgcgtgacca cgaacacagc catcccgaag
      961 ggcggaggat cgccccacgg ctccacgccg acatcgaaca tccgcttgcc gatcaggacc
     1021 gctccggtgg tcgcgtagat ctcatcgacg atctcggcgt cggtggcggt cttcgcggag
     1081 aacatccaat cgtgcaggcg gccggggtca tcgcccaaag ggttgtcgcc tcccgggtcg
     1141 ggttcggtga cgaaaccatc cagcgacatc gagatgtcgg ccgtgacctt gctcatcatg
     1201 atgccctctc ggcgcccccg gcaggattcg aacctgcgcc cccggctccg gaggccggtg
     1261 ctctatcccc tgagctacgg gggctcgtgc tgggaagtgt acccaccgag ggtcaccccc
     1321 cgagcccgtg tgtcccacgt ggcggtggca tgacccacga tcgcgagcgg acccacgcac
     1381 acgggcgccg gtaccatccg gacatgccgc gggttctgtt cgcagacgat gacccgatcc
     1441 tgcaccggct cctcgaggtc aacttccggg tggtggggat cacgatcgag tcggtgggca
     1501 gaggcgacgc cgcgctggag cgggtcgtgg ctgcgcctcc cgacgcgctg gtcctcgacg
     1561 cgacgatgcc ggggatggac gggcacgagg tgtaccggcg cctccgcgag gtgcccgcgc
     1621 tcgcggaact tccggtgatc ttcctcacgg gacgcggcgc cgaagagttc gaggcgtacg
     1681 cgggaccccg cgtgcggatc atgaccaaac ccttcgaccc caccgagctc gtccaggtgg
     1741 tccgcgaggc gatgggggaa gggggatgat cgaggagcgc cttaggaacc tcctcgcgga
     1801 cgcgatcgcg aaggccgcgc cgtcgctcgg cgcggcgaca ccgttgcccg taccggagct
     1861 gcagcgacct cgccagaagg ggttcggcga cttcaccacg aacgtcgcga tggtcatcgc
     1921 gtcgcacgtg ggagccgatc cgcgcgcgac ggccgccgcg atcgtggagg cgatgccatc
     1981 cgcgacgttc ctcgcgaagg cggaggtggc ggggcccggc ttcatcaacc tgtggaccac
     2041 c
//
LOCUS       DASUVE010000258         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_879129, whole genome shotgun sequence.
ACCESSION   DASUVE010000258 DASUVE010000000
VERSION     DASUVE010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879129"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VF036_05610"
     CDS             <1..753
                     /locus_tag="VF036_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HEX6264737.1"
                     /translation="PEGRASLVPAPPWHYSGDVMTIEYRTDPARVAALLPDGVDPAED
                     DPGAVAIVWADWQSCSDSREELLDPVRSQYKECFVVVRCRYEGDTYSRCVYIWVDKDF
                     ALARGWYQGYPKKLGSIWMTRPVSVGQAGPRLGAGAVFGATLQANDRRLADARLTLTG
                     GSETAGFVNALPMLHSRWMPSIEKEDGDSLAELVTMRSRDVDLGPAFTGEADIRLFDA
                     PGEELAALEPSEMIAGYWRQVGATFDGGSRLA"
     gene            756..1517
                     /locus_tag="VF036_05615"
     CDS             756..1517
                     /locus_tag="VF036_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6264738.1"
                     /translation="MRLARFRYGDRIATGAMDRGSEEIRILRGTFFEDPLPTGETVAL
                     SDVHLLAPVLPSKLVCVGRNFVAHAEEWGLAVPEEPLLFLKPSTAVIGPRDPIPLLPI
                     SRRIDYEGELGVVIGRLARGVRAEEAFRYILGYTCGNDVTLRDLQKKDDQWARAKGFD
                     GSAPLGPWIETELDPNDAIVRTRLNGEIRQQASTADMVFGVATLIEYTTTFMTLLPGD
                     VLLAGTPEGVGKLADGDVVEVEVEGVGVLRNEVRG"
     gene            1514..>2743
                     /gene="gltX"
                     /locus_tag="VF036_05620"
     CDS             1514..>2743
                     /gene="gltX"
                     /locus_tag="VF036_05620"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX6264739.1"
                     /translation="MSVRCRFAPAPSGSIHVGNARSALFSWLYARHEGGTFVLRLEDT
                     DASRVTEEASQGVVEDLRWLGLAWDEGPDVGGPYGPYRQSERSGIYREMTDRLLEGGH
                     AYRCWCTAEELDERRKAALARGQPPGYDGRCRQRPAAEIAAFEAEGRPFAIRFLMPDQ
                     GWVVDDLVKGEVRWGSNDLRDFVIVRSDGSPVFLLAVAVDDLLMEITHVIRGDDLLAS
                     APRNAAVIEALGGTPPAYAHLPQVLGPDSKPLSKRHGDTSVAAFREHGFLPEAMVNYL
                     ALLGWAKDDHTTLLTRDELMDAFDLSHVSHNPAVFDVQKLEWMNNHYIQSLDEEELAA
                     RCVHFLTEEGLSLDPDLLRRAMPIVRERMKTLTEAPILLRFLFTDDVRPNEKATDLIA
                     KAPEGYLKEAAEALDALE"
BASE COUNT          424 a          935 c          965 g          419 t
ORIGIN      
        1 cccgagggca gggcgtcgct cgtcccggca ccaccgtggc actactccgg cgacgtgatg
       61 acgatcgagt accgcactga ccccgctcgc gtcgcggcgc tccttccgga cggtgtcgac
      121 cccgccgagg atgaccccgg cgccgtggcg atcgtctggg ccgactggca gtcgtgcagc
      181 gactcccgcg aggaactgct cgaccccgtg cgctcgcagt acaaggaatg cttcgtggtg
      241 gtccgctgcc ggtacgaggg ggacacgtac tcgcggtgcg tctacatctg ggtcgacaag
      301 gacttcgcgc tcgcgcgggg ctggtaccag gggtacccga agaagctcgg ctcgatctgg
      361 atgacgcggc cggtctcggt cggccaggcg gggcctcggc tcggcgccgg ggcggtgttc
      421 ggcgcgacgc tccaggcgaa cgaccgacgc ctcgccgacg cccgcctcac gctgacgggt
      481 ggctcggaaa cggcagggtt cgtcaacgcc ctcccgatgc tgcactcgcg ctggatgccc
      541 tcgatcgaga aggaggacgg ggactcgctc gcggagctcg tcacgatgcg ctcgcgcgac
      601 gtcgatctcg gaccggcgtt cacaggggag gcggacatcc ggctgttcga cgcgccgggg
      661 gaggagctcg cggccctcga gccctccgag atgatcgcgg gctactggcg tcaggtcggc
      721 gcgacgttcg acggcgggtc caggctcgcc taggtatgcg actcgcccga ttccgatacg
      781 gcgaccgcat cgccaccggc gcgatggatc gcggatccga ggagatccgg atcctccgcg
      841 gcacgttctt cgaggatccc cttcccacgg gcgagaccgt cgcgctgagc gacgtccacc
      901 tgttggctcc ggtcctcccg tcgaagctgg tgtgcgtcgg caggaacttc gtggcccacg
      961 cggaggagtg ggggctcgcg gtgcccgagg agccgttgct gttcctgaag ccctcgaccg
     1021 cggtgatcgg cccccgcgat ccgatcccgc tgctcccgat cagccggcgg atcgactacg
     1081 agggggagct cggcgtggtg atcgggcggt tggcgcgcgg tgtgcgtgcc gaggaggcgt
     1141 tcaggtacat cctcggctac acgtgtggaa acgacgtgac gctccgcgac ctccagaaga
     1201 aggacgatca gtgggcgagg gcgaaggggt tcgacgggtc ggcgccgctc ggaccgtgga
     1261 tcgagacgga actggacccg aacgacgcga tcgtgcggac ccggctgaac ggcgagatcc
     1321 gtcagcaggc ctccaccgcc gacatggtct tcggcgtcgc gaccctgatc gagtacacca
     1381 ccaccttcat gacgctgctc cccggggacg tcctgctcgc cggcacgccg gaaggggtcg
     1441 gcaagctcgc cgatggagac gtcgtggagg tcgaggtcga aggcgtgggc gtcctccgga
     1501 acgaggtccg cgggtgagcg tccggtgccg attcgcaccg gccccctcgg gctcgatcca
     1561 cgtcgggaac gctcgcagcg cgctgttctc ctggctctac gcgcgtcacg agggcgggac
     1621 gttcgtcctc cggctcgagg acacggatgc gagccgtgtg acggaggagg cctcccaggg
     1681 cgtcgtcgag gacctccgtt ggctcggtct ggcctgggac gagggcccgg acgtcggagg
     1741 cccgtacggg ccgtaccggc agtcggagcg atcgggcatc taccgcgaga tgaccgaccg
     1801 gctgctcgag ggagggcacg cctaccggtg ctggtgcacg gccgaggagc tcgatgagcg
     1861 tcggaaggca gcgctcgcac gaggccagcc acccggctac gacggccggt gccgccagcg
     1921 cccggcggcg gagatcgccg cgttcgaggc ggaggggaga ccgttcgcca tccgcttcct
     1981 gatgccggat cagggatggg tcgtcgacga tctcgtgaag ggcgaggttc gatggggatc
     2041 gaacgacctc cgagacttcg tgatcgtccg ttccgacgga tcgcccgtgt tcctcctggc
     2101 cgtcgccgtc gacgacctcc tgatggagat cacccacgtg atccgcggcg atgacctgct
     2161 cgcgagcgca ccccgcaacg ccgcggtgat cgaggcactg ggggggacac ctcccgccta
     2221 cgcgcacctc ccacaggtcc tcggacctga ctccaagccc ctgtctaagc ggcacggcga
     2281 cacgagcgtc gccgccttcc gcgagcacgg gttcctcccc gaagcgatgg tcaactacct
     2341 ggcgctcctc ggctgggcca aggacgatca cacgaccctc ctgacccgcg acgagctcat
     2401 ggacgccttc gatctctccc acgtctccca caacccggcc gtgttcgacg tccagaagct
     2461 cgagtggatg aacaaccatt acatccagtc actcgacgag gaggagctcg ccgcgcgatg
     2521 cgtgcacttc ctcaccgagg aggggctctc cctcgatccg gacctgctgc ggcgcgcgat
     2581 gccgatcgtc cgcgaacgga tgaagaccct cacggaggcg ccgatcctgc ttcggttcct
     2641 cttcacggac gacgtccggc ccaacgagaa ggcgacggat ttgatcgcga aggcccccga
     2701 ggggtacctc aaggaggcgg ccgaagcgct cgatgccctg gag
//
LOCUS       DASUVE010000259         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_693180, whole genome shotgun sequence.
ACCESSION   DASUVE010000259 DASUVE010000000
VERSION     DASUVE010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693180"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="VF036_05625"
     CDS             complement(<1..1275)
                     /locus_tag="VF036_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6264740.1"
                     /translation="MAINTYDAIVIGGGHNGLTAGAYFARHGAKTVVLEARHKAGGAA
                     DTSAPFADHPEINVTTYSYVMSLMPPTIINELQLKKHGYDVTPFGPYFQAFPDGRAIM
                     VYADEAERSRDSIAQFSKKDADALPEWEAWLKGVADILGPLLLQSPPRAGSLAIGDLL
                     ETARTAWKVRKLGVRGVGDVTRLFTMSITDLLDDWFESDAIKGMLAVNGIIGTWAGPD
                     EPGTAYVMLHHSIGDVGDGHLGSWGYQQGGMGAVSDSIRRSAEAFGAEIRTNARVKSM
                     LTSGGRFHGVVLEDGTELRAPIGVTCVHPKIGFLDMIPPTDLPADFVTDIQRWKTRSG
                     VVKINVAMSELPDFTAMPGKELQEHHTGSVELCFSPQYAERAFQDSHMDRKASVAPFV
                     DGVIPTTMDKKLAPEGTHVFSMFSQWVPDDWVH"
     gene            1478..2140
                     /locus_tag="VF036_05630"
     CDS             1478..2140
                     /locus_tag="VF036_05630"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6264741.1"
                     /translation="MAKERAKPKPGAKAGPKVGKDRRQEILAAAAQVITDRGLAETRI
                     QDIAETCGVSTGLILYYFESKDRLLVEALTYANDQFYLRLSRELRKNPSARAQLDRLI
                     ELSVPGLLPEFSFLDEWALWVEIWVRALRDPEMAKEHEVLNRRWRRSIADIVRHGRST
                     GEFPEGLDADELGIQIGAVVDGLAIQVLMDHGDVSPDDMLRVSRDVSAKLIGFALEPA
                     GA"
BASE COUNT          360 a          764 c          778 g          382 t
ORIGIN      
        1 gtggacccag tcgtccggca cccactggct gaacatcgag aacacgtgcg ttccctcggg
       61 ggcgagcttc ttgtccatcg tcgtggggat cacgccgtcg acgaacgggg ccaccgaggc
      121 cttccggtcc atgtgggagt cctggaacgc gcgctccgca tattgcggtg agaagcacag
      181 ctccacggag ccggtgtggt gctcctgtag ctccttcccg ggcatcgccg tgaagtccgg
      241 cagctccgac atcgcgacgt tgatcttcac gacgcccgag cgtgtcttcc accgctggat
      301 gtccgtgacg aagtcggccg gcagatccgt gggcgggatc atgtcgagga acccgatctt
      361 cgggtgcacg cacgtcacac cgatcggggc acggagctcg gtcccgtcct cgagcacgac
      421 gccgtggaac cgacccccgg acgtgagcat cgacttcacg cgcgcgttcg tgcggatttc
      481 ggcgccgaac gcctccgcgg agcgccggat cgaatcggag acggcgccca tgccgccctg
      541 ctggtagccc caggatccga ggtgaccgtc gccgacgtcg ccgatcgagt ggtggagcat
      601 cacgtacgcg gtcccgggct cgtccggccc cgcccacgtc ccgatgatgc cgttcaccgc
      661 gagcatgccc ttgatcgcgt cggactcgaa ccagtcgtcc agcaggtccg tgatgctcat
      721 cgtgaacagc cgcgtgacgt cgccgacgcc ccggaccccg agcttgcgga ccttccacgc
      781 ggtgcgcgcc gtctcgagca agtcgccgat cgcgagcgag ccggcacggg gcgggctctg
      841 gaggagcagg ggcccgagga tgtcggcgac gcccttcagc cacgcctccc actcagggag
      901 cgcgtccgcg tccttcttcg agaactgcgc gatcgagtcg cgcgagcgct cggcctcgtc
      961 ggcgtagacc atgatcgcgc ggccgtcggg gaacgcctgg aagtagggac cgaacggcgt
     1021 gacgtcgtag ccgtgcttct tgagctggag ctcattgatg atcgtcggcg gcatcagcga
     1081 catcacgtag gagtaggtgg tgacgttgat ctccgggtga tccgcgaacg gagcgctcgt
     1141 gtcggcggcg ccacccgcct tgtgcctcgc ctcgaggacc acggtcttgg cgccgtgccg
     1201 ggcgaagtag gcccctgccg tcagcccgtt gtgccccccg ccgatcacga tcgcgtcgta
     1261 ggtgttgatc gccatgtgcc ttcctttcct ggcccgcctc cgcccgagtc ggaccggaag
     1321 ccctgcgaat gatgccctat tcctgaacgg tgagtcaaac gccctcgtgg gagccctggc
     1381 tttgatttct gctgctcaac ggggtacggt tcgcggggcc accggggtgc cggcggacaa
     1441 cgacccgcat gatctctgta caccgttcag gagttcgatg gcgaaggaac gagcgaaacc
     1501 gaagccgggt gccaaggccg ggcctaaggt cggaaaagac cgcagacagg agatcctcgc
     1561 ggccgcggcg caggtcatca cggatcgggg cctcgccgag acgaggatcc aggacatcgc
     1621 cgagacgtgc ggcgtgtcca ccgggctgat cctctactac ttcgagtcga aggaccggct
     1681 cctggtcgag gcgctcacgt acgcgaacga ccagttctac ctccgcctgt cgcgggagct
     1741 gcgcaagaac ccatcggcgc gtgcgcagct cgaccggttg atcgagctct cggtcccggg
     1801 tctgctcccc gagttctcct tcctcgacga atgggcgctc tgggtcgaga tctgggtccg
     1861 ggcgctccgc gatccggaga tggcgaagga gcacgaggtg ttgaaccgcc ggtggcggcg
     1921 gtcgatcgcg gacatcgtgc gtcacggccg ctcgaccggc gagttcccgg agggactcga
     1981 tgccgacgaa ctcgggatcc agatcggcgc cgtcgtcgac gggctcgcga tccaggtgct
     2041 gatggatcac ggcgatgtgt cccccgacga catgctccgg gtgagccgcg acgtctcggc
     2101 gaagttgatc gggttcgccc tcgagcccgc cggcgcgtag cgacccatag ctgtaggggc
     2161 gaccgaggtc tacgctgagg acctgatccg ttggctgcga acgcggctgc gccgtcgaac
     2221 ggcccgactc gatcccggga tcgtgcgacg agcccgcggc gatgccgatc gcgccaactg
     2281 gaag
//
LOCUS       DASUVE010000260         2055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1068855, whole genome shotgun sequence.
ACCESSION   DASUVE010000260 DASUVE010000000
VERSION     DASUVE010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068855"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..262)
                     /locus_tag="VF036_05635"
     tRNA            complement(191..262)
                     /locus_tag="VF036_05635"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(228..230),aa:Thr,seq:cgt)
     gene            379..>2055
                     /locus_tag="VF036_05640"
     CDS             379..>2055
                     /locus_tag="VF036_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX6264742.1"
                     /translation="MAITETDPIGPVHRVMVGTDRSETATRAVAWAASFAERFGAELH
                     VVQVIVPEHAADTEHGAAEATRARSAADELQVYTTQLAGERGHAHVVIDDDPAMAIVR
                     SSEDHAVDVLIVGNAGMAGRKEFLLGNVPNRISHNARCTVIIVNTTNGRTTAIRGKGT
                     AAAIRSSSVEPVIHPHLVARGTKIGVVFAKHGLRELFGRPDEEGAVGRRRQAKRLRAA
                     LEDLGPTFCKLGQILSTRPDLLPPEFIEELATLQDNVPPLSEAQVVAMMEQELGVPWE
                     DVFETVEPQPLAAGTIAQVHRASLATGQKVVIKIQRPDARELIEQDLALLEVFAEKAA
                     KRPSLKLVIDMEAVFQHLSDSLQRELDFRQEASNMERIGEVLGSFPRLAVPDVHHDLS
                     TSRLLVMHDVGGGPIDTAPEGAPRREAARQLLESFYKQIMVYGFFHADPHPGNLMWQP
                     EEERLYFLDLGMVGEVGAEMREQLLLLLMAFSQDDVGFLTDVTLMLANAIERSDLDVD
                     RFQTEIGSIMARHRTSSLKDIQLGPILQEMTEISVRHGVPLPASLALTGKA"
BASE COUNT          338 a          709 c          702 g          306 t
ORIGIN      
        1 acccagccgc ccgtgttgag ctcggggctg atggaggggt cgatcggcgt ctgggcgaac
       61 gcgacgcctg ccgaaaggac gaggaggacg ggagcaagca cgatcgctcg gaaggccctc
      121 atccgccgcg attctcccaa cctgctcgct cctttcgcaa ctcctcggtg tggactccgt
      181 ggtctcaggg agccggagat gggattcgaa cccatgacct accacttacg aggcggttgc
      241 tctaccactg agctactccg gcggacgagg caggatacca acggccctga tcgggacggg
      301 tgcgggtgct gccatcacct cgtgtcgaca gggctccccc gttggctgcg agcccgggag
      361 gcgccatgat gcgacccgat ggcgatcacc gagaccgacc ccatcggccc cgttcaccgc
      421 gtgatggtgg gcacggaccg ctccgagacg gcgaccaggg ccgtggcgtg ggccgcgtcg
      481 ttcgccgaac ggttcggcgc cgagctccac gtcgtccagg tgatcgtccc ggagcacgcc
      541 gccgacacgg agcacggcgc cgccgaggcg acccgggccc gcagcgcggc cgacgagctg
      601 caggtctaca ccacgcagct cgccggtgaa cgcggacacg cccacgtcgt gatcgacgac
      661 gatcccgcga tggcgatcgt gcgctcctcc gaggaccacg cggtggacgt cctgatcgtc
      721 ggcaacgccg gcatggccgg gcggaaggag ttcctcctcg gcaacgtgcc caaccggatc
      781 agccacaacg cccggtgcac ggtgatcatc gtgaacacga cgaacggtcg aacgacggcg
      841 atccgaggca agggcacggc cgctgcgatc cgctcctcct ccgtcgagcc cgtgatccat
      901 ccgcacctgg tggcgcgagg gaccaagatc ggcgtcgtct tcgccaagca cggcctgcga
      961 gagctgttcg gtcgtcccga cgaggagggg gcggtgggcc gccggcgcca ggcgaagcgg
     1021 ctccgggccg cgctcgagga tctcggtccc acgttctgca agctcgggca gatcctctcg
     1081 acccggccgg acctccttcc ccccgagttc atcgaggaac tcgcgaccct gcaggacaac
     1141 gtgccgcccc tgtcggaggc acaggtcgtc gccatgatgg agcaggagct cggggtgccc
     1201 tgggaggacg tgttcgagac cgtggaaccc cagccgctcg ccgccgggac gatcgcgcag
     1261 gtccaccgcg cctcgctcgc cacgggacag aaggtcgtga tcaagatcca gcgtcccgac
     1321 gctcgggagc tgatcgaaca agacctggcg ctgctcgagg tcttcgcaga gaaggccgcg
     1381 aagcgcccga gcctgaagct cgtgatcgat atggaggccg tcttccagca cctgtcggac
     1441 tcgctccagc gagagctcga cttccgtcag gaggcgtcga acatggagcg gatcggcgag
     1501 gtgctcgggt cgttcccgcg cctcgcggtg cccgatgtcc accacgacct gtcgacgtcc
     1561 cgcctcctcg tgatgcacga cgtgggtggc gggcccatcg acacggcgcc ggagggtgcg
     1621 ccgcggcgcg aggccgcccg tcagctgctc gagtccttct acaagcagat catggtctac
     1681 ggcttcttcc acgccgaccc gcaccccggg aacctgatgt ggcagccgga ggaggaacgt
     1741 ctctacttcc tcgacctcgg gatggtcggc gaggtgggag cggagatgcg cgagcagctg
     1801 ctgctgctgc tgatggcgtt ctcccaggac gacgtggggt tcctcaccga cgtcacgctg
     1861 atgctcgcga acgcgatcga gcggagcgac ctcgacgtgg atcggttcca gacggagatc
     1921 ggctcgatca tggcccgcca ccggacctcg tcgctgaagg acatccagct cggcccgatc
     1981 ctccaggaga tgaccgagat ctcggtccgc cacggcgtgc cgttgccggc gtcgctcgcg
     2041 ctcacgggga aggcg
//
LOCUS       DASUVE010000261         3476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_693482, whole genome shotgun sequence.
ACCESSION   DASUVE010000261 DASUVE010000000
VERSION     DASUVE010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693482"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VF036_05645"
     CDS             complement(<1..374)
                     /locus_tag="VF036_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6264743.1"
                     /translation="MRERSIIVTGGAGFVGSHLCDRLLAEGHQVVAVDDLVTGRAENL
                     SAASRHGDAFAFSETDIRTPGLFDVFDRHRPDVVMHLAAQSGVRPSLEDPDHDASVNV
                     LGTLNVLRCATSVGCRKVVYAAS"
     gene            414..1388
                     /locus_tag="VF036_05650"
     CDS             414..1388
                     /locus_tag="VF036_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264744.1"
                     /translation="MAAFEQPQIGESQPGSHGRTSSEVLLTVVRSRGGRRTHLSACLV
                     IAIVLAYQMATSGAIIAAPQGRLVRLIRTTPFTNKPVSMKDAEGSAYVPRDHSLWLAD
                     DYGRRIYEVNPSTGALKRVIGQKKLASARRLGGGTKAGIDRTRDLESIAYDRVRDALY
                     VFSGPCCSSSVKPAAFRLTRTPAGRLRVESFQPLPGSANFTAAAWNAGNRTIYVGQGR
                     DLRAYRYRTNHLGRPVHVPNLSGITGMSFSPNGASLFVTTSDEQLLRVRWPRKELVAG
                     WVLELAAFGVGDARAVELIDWRYFVLDGNPARPDTDPRKYAVYVFRMS"
     gene            complement(1385..1522)
                     /locus_tag="VF036_05655"
     CDS             complement(1385..1522)
                     /locus_tag="VF036_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264745.1"
                     /translation="MPGGIDDARGTDADAGEYPTAFFRGLAVAIVASAPLWVAVIRVV
                     R"
     gene            1682..2491
                     /locus_tag="VF036_05660"
     CDS             1682..2491
                     /locus_tag="VF036_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005518608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX6264746.1"
                     /translation="MAEGDTVFATAARMHHALVGQVLTSTDLRVPRYATADLSGQIVR
                     EVGARGKHLFIRTDRGISVHSHLGMDGSWRLYGPGERWRGRAFEVRAVLTIEERFAVG
                     HRLRRLEVLRTTAEPELLAHLGPDVLGSTWDAAEATRRLVASSDREVGDAVVDQSVMA
                     GPGNVYRSEVCFLARVDPRTAVGSIADPLAIVELMRALMDRNRTGDRRVTTDDPRPGH
                     ELWVYGRGGRPCRRCGTPIRSFARGPRSQERIIFVCPRCQPVVGPSGHDES"
     gene            2554..>3476
                     /locus_tag="VF036_05665"
     CDS             2554..>3476
                     /locus_tag="VF036_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6264747.1"
                     /translation="MGPEAGLIEKEPRPSVTDIDGERLFAAAPEALFVVRGDRIELAN
                     DQTEAFLGCDPVGLDVHEVIPGWVEGADAGVPFEASLRSPAGGELPGEVRVQRLDPTT
                     AIVAIRDARALLAGRDAEAALTEAEARYRSLVEQIPAVVYADDGDVTTYVSPQIQQIL
                     GVSPEAYREDPGMWLRMVHPDDRVQVEAESAAFLAGEGGDLTDYRMIRPDGRIVWVRD
                     RAFAFRDTDGQVIWEHGLLFDVTELKEAEARVTEMAFHDGLTGLANRELFEETLVLAV
                     ERARRTEALVAVLYFDLDNFKLVNDSLGHHAG"
BASE COUNT          549 a         1194 c         1205 g          528 t
ORIGIN      
        1 gaagccgcgt acaccacctt ccgacaaccg acggacgtgg cgcagcggag cacgttgagg
       61 gtcccaagga cgttgacgct cgcatcgtga tccgggtcct cgagcgacgg ccggacgccg
      121 gactgcgcgg ccaggtgcat caccacgtct gggcgatgac gatcgaacac gtcgaacagg
      181 cccggtgttc ggatatcggt ctccgagaag gcgaaggcgt ccccgtggcg ggaagcggcc
      241 gagagattct ccgcgcggcc cgtcaccagg tcgtccaccg ccacgacctg gtgaccctcg
      301 gccaggaggc gatcgcacag atgggatccg acgaatccgg cgccgccggt tacgatgatc
      361 gagcgctccc gcatcgaggg accatagcaa gcagtgatcg tcgtctcggc gtcgtggccg
      421 cgtttgagca gccccagatc ggggaatcgc agcccggatc ccacggccgg acgtcatccg
      481 aggtgttgct cacggtggtc agaagtcgag gtggtcggcg cacgcacctt tccgcctgcc
      541 tcgtgatcgc gatcgtcctg gcgtaccaga tggccacgtc cggtgcgatc atcgccgccc
      601 cccaggggcg cctggtcaga ctgatccgaa cgacaccgtt cacgaacaag cccgtctcca
      661 tgaaggatgc cgagggcagt gcctacgtgc cgcgcgatca ctccctctgg ctggcggatg
      721 actatgggcg gcgcatctac gaggtgaacc cttccacggg cgccctgaaa cgcgtgatcg
      781 gccagaagaa gctcgcgtcg gcgcggcggc tcggcggcgg caccaaggcc gggatcgacc
      841 gcacgcggga tctggaaagc atcgcctacg accgtgtccg ggacgctctc tacgtcttct
      901 ccgggccgtg ttgctcgtcg tcggtgaaac ccgcagcctt ccggctcacg cgcacgcccg
      961 ccgggcgtct ccgtgtcgag tccttccagc cgctgccggg atccgcgaac ttcacggcag
     1021 ccgcgtggaa cgccggcaac cgcacgatct acgtgggaca gggccgcgat cttcgcgcct
     1081 accgctaccg gacgaaccac ttggggcgtc ccgtccatgt cccgaacctc tcggggatca
     1141 ccggcatgag cttcagcccg aacggcgcca gcctcttcgt gacgacgtcc gatgagcagc
     1201 tcctccgggt gcgctggccg cggaaggaac tcgtcgccgg atgggtgctc gaactcgctg
     1261 ccttcggggt cggtgacgct cgtgcggtcg agctgatcga ttggcgctat ttcgtcctcg
     1321 atggcaaccc cgctcgcccg gatacggatc cacgcaagta cgcggtctac gtgttccgga
     1381 tgagctagcg aaccacccgg atcaccgcga cccacagggg ggcgctggcg acgatcgcca
     1441 ccgcgagacc gcggaagaac gcggtcggat actctccagc atcagcgtcc gtgccacgag
     1501 catcgtcgat cccgccaggc atcgatccca tcctttcgcg cactcggctc gccctcgagt
     1561 agaggagtac cgcaccagta gagacgaccg aggtcgagga ggtgaacgga gagcgaactc
     1621 gaggcgatcg gggtgcatcg gttcttgagg atccgcgccc tcgcctcgag gttcggttgg
     1681 gatggctgag ggcgacaccg tcttcgcgac ggcggcgcgg atgcatcacg cgctcgtggg
     1741 acaggtgctg acctcgaccg acctccgcgt accgcggtac gcgaccgccg acctgtcggg
     1801 gcagatcgtc cgagaggtgg gcgcgcgagg gaagcatctc ttcatccgaa cggaccgcgg
     1861 catctccgtg cactcgcatc tcgggatgga cggctcgtgg cggctgtacg ggccgggcga
     1921 gcggtggcga ggtcgggcgt tcgaggtgcg ggccgtgctc acgatcgagg agcggttcgc
     1981 ggtcggccac cggctgcgac ggctcgaggt gctccggacc acggccgagc cagagctcct
     2041 ggcccatctc ggcccggacg tgctgggatc cacctgggat gcggccgagg cgacccgccg
     2101 cctcgtcgcg tcttcggacc gggaggtggg cgacgcggtc gtggatcagt cggtgatggc
     2161 cgggccgggg aacgtctacc gctcggaagt gtgcttcctg gcacgggtgg acccgcggac
     2221 cgcggtcggc tcgatcgcgg atccgctcgc gatcgtcgag ctgatgcggg ccctgatgga
     2281 tcggaaccgt accggcgacc gccgcgtcac cacggacgac ccgcggcccg gccacgaact
     2341 ctgggtctac ggccggggcg ggagaccctg tcgtcgctgc ggcacaccga tccgctcctt
     2401 cgcccgagga cctcggtcgc aggagaggat catcttcgtc tgcccgcgat gccagcccgt
     2461 cgtcggcccc tccggccacg acgagtccta gcgttcccgg tcgcgttcct tcatccttgc
     2521 cgggaaccat cggcgggtgg tcgtcccgcc cacgtgggcc cggaggcagg attgatcgag
     2581 aaggaacctc ggccgtcggt gaccgacatc gacggggagc gcctgttcgc ggccgccccc
     2641 gaggccttgt tcgtcgtccg cggcgaccgg atcgagctcg cgaacgacca gaccgaggcg
     2701 ttcctcggct gcgaccccgt gggactcgac gtccacgagg tgatccccgg atgggtcgag
     2761 ggcgccgacg ccggcgtccc gttcgaggcg tccctcagga gccccgcagg tggcgagctg
     2821 cccggcgagg tccgcgtcca acgactggat cccaccaccg cgatcgtcgc gatccgcgac
     2881 gcccgggcgt tgctcgccgg gcgcgacgcc gaggccgccc tgacggaggc ggaggcccgg
     2941 taccgaagcc tggtcgagca gatccccgcc gtggtctacg ccgacgacgg cgacgtcacg
     3001 acgtacgtga gtccacagat ccagcagatc ctgggggtct ccccggaggc ctaccgagag
     3061 gatccaggga tgtggctccg gatggtccat cccgacgacc gggtccaggt ggaggccgag
     3121 agcgccgcat tcctcgccgg cgagggcggc gacctcaccg actaccggat gatccgcccc
     3181 gacggcagga tcgtatgggt gcgcgaccgg gccttcgcgt tcagggacac ggacgggcag
     3241 gtcatctggg agcacgggct cctgttcgac gtcaccgagc tcaaggaagc cgaggcccgc
     3301 gtgacggaga tggcgttcca cgacggactc accggcctcg cgaaccgaga gctcttcgag
     3361 gagaccctcg tcctggcggt ggagcgggcc agacgcaccg aggcgctggt cgcggtcctg
     3421 tacttcgacc tcgacaactt caagctggtg aacgactcgc tcgggcacca cgccgg
//
LOCUS       DASUVE010000262         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_316879, whole genome shotgun sequence.
ACCESSION   DASUVE010000262 DASUVE010000000
VERSION     DASUVE010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316879"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VF036_05670"
     CDS             <1..1026
                     /locus_tag="VF036_05670"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX6264748.1"
                     /translation="VEVAPRQILKRQLERAAKVGYTIKTGSELEFYLFKDGYDELAER
                     GYRDLRPSSPYIMDYHMLQTTRDEWVIRQIRNGMLGAGIPIEFSKGEFGKGQHEINIT
                     YSDALSNADHHALYKNGVKEIAELNGVAATFMAKWTMAEAGSSCHLHSSVWSADGQES
                     LMWDDDDANHMSPTFKHMLGGMMSTAREMAWMYAPFVNSYKRYQLGSWAPTAILWSHD
                     NRTCGFRVVGEHQGNRVESRLPGGDVNPYLGYAATIAAGLHGIQNQVECPPLFEGNAY
                     ESKDAERVPSSLHEAIAEMERSDVMREAFGDFAFGHLVNMARQEQIIFDNNTVTDWEL
                     IRYFERG"
     gene            1064..1450
                     /locus_tag="VF036_05675"
     CDS             1064..1450
                     /locus_tag="VF036_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264749.1"
                     /translation="MKRATLIVLCVLLSACFSESGLTDEFQDATNAIDDLVDAIADEG
                     PVRDAVARVRDTVDEADAALEAYRENPNAETRQALEDAEQRMSDAREELEGLVDSAPE
                     PVRDAIRTVIDRLANLRDDVARELED"
     gene            complement(1456..>2293)
                     /locus_tag="VF036_05680"
     CDS             complement(1456..>2293)
                     /locus_tag="VF036_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX6264750.1"
                     /translation="LSVATVVAIGCGDEMAATLGAGVFEPGEVCDVVGTAEPVCAASA
                     EPREDPTMLVECHPHADPDAWLLENPGFVSGGNLRWWRDQFAPDERRNEERGLGDAYD
                     QLSEEASRIPPGSDGVVFLPAMQGAMAPEWNGAARGVFYGLTLAHTRDHMTRAILEGS
                     AFGLRDILEAMKAAGLGVRRLTIVGGGAKGPLWRQIKADVTGLPVRVPESVETTATGA
                     AILAAVGSGVHATVAEAVDSFVSFRPEEHEPDPSARDAYDEAYQRYREVYFALKPVFE
                     PD"
BASE COUNT          397 a          784 c          781 g          331 t
ORIGIN      
        1 gtcgaggtcg ctccccgaca gatcctgaag cgtcagctgg agcgggccgc gaaggtcggc
       61 tacacgatca agacggggtc cgagctcgag ttctacctgt tcaaggacgg ctacgacgag
      121 ctcgcggagc gcggctaccg cgacctgcgc cccagcagcc cgtacatcat ggattaccac
      181 atgctccaga ccacccggga cgagtgggtt atccggcaga tccgcaacgg gatgctcggc
      241 gccggcatcc cgatcgagtt cagcaagggc gagttcggca aagggcaaca cgagatcaac
      301 atcacgtact cggacgccct ctcgaacgcc gaccaccacg cgctctacaa gaacggcgtg
      361 aaggagatcg cggagctgaa cggcgtcgcc gcgacgttca tggcgaagtg gacgatggcc
      421 gaggccggct cctcgtgcca cctccactcg agcgtgtgga gcgccgacgg ccaggagtcg
      481 ctgatgtggg acgacgacga cgcgaaccac atgagcccga cgttcaaaca catgctgggc
      541 gggatgatgt ccaccgcgcg tgagatggcg tggatgtacg cgccgttcgt gaactcctac
      601 aagcgctacc aactcgggtc gtgggcgccg accgcgatct tgtggagtca cgacaatcgt
      661 acgtgcggct tccgggtggt cggcgaacat caggggaacc gtgtcgaatc ccggctgccg
      721 ggcggagacg tcaatccgta cctcgggtac gcggcgacga tcgctgccgg cctgcacggg
      781 atccagaacc aggtcgagtg ccccccactg ttcgagggga acgcctacga gtcgaaggac
      841 gcggagcgcg tgccgtcctc gctgcacgag gcgatcgcgg agatggagcg ctcggacgtg
      901 atgcgggagg ctttcggcga cttcgcgttc gggcatctcg tgaacatggc ccgccaggag
      961 cagatcatct tcgacaacaa cacggtcacg gactgggagc tgatccggta cttcgaacgc
     1021 ggctgatgct tgccgacgcc cgccggcggg tacgggtcgg agcatgaaga gagccaccct
     1081 gatcgtgctg tgcgtgctcc tgtccgcctg cttctccgag agtggcctca cggacgagtt
     1141 ccaggatgcc acgaacgcga tcgacgatct cgtcgacgcg atcgcggacg aggggccggt
     1201 ccgcgacgcc gtcgcccggg tccgtgacac cgtcgacgag gcggacgccg ccctggaggc
     1261 gtaccgcgag aacccgaacg ccgagacccg ccaagcactc gaggatgcgg agcagcggat
     1321 gagcgacgcc cgggaggagc tcgaggggct ggtggacagc gcccccgaac ccgtccgtga
     1381 cgcgatccgg accgtcatcg accgtctggc gaacctccgc gacgacgtcg cgcgcgagct
     1441 cgaggactga tccgttcagt ccggttcgaa cacgggtttc agagcgaagt acacctcgcg
     1501 gtagcgctgg tacgcctcgt cgtacgcgtc gcgggccgac gggtcgggct cgtgctcctc
     1561 aggccggaac gacacgaatg aatccaccgc ctccgccacg gtcgcgtgca cgccggagcc
     1621 gacggctgcg aggatcgcgg cgccggtggc ggtggtctcc acgctctccg ggacccgaac
     1681 ggggagaccc gtgacgtccg ccttgatctg ccgccagagc ggtcccttgg cgccgccccc
     1741 cacgatcgtg agccgccgga cgccgaggcc ggcagccttc atcgcttcga ggatgtcgcg
     1801 caggccgaac gcgctcccct cgaggatcgc tcgggtcatg tgatcgcgcg tgtgggcgag
     1861 ggtgagcccg tagaacaccc cccgggcggc gccgttccat tcgggcgcca tcgccccctg
     1921 catcgccggg aggaagacga cgccgtcgct cccgggaggg atccgcgacg cctcctcgct
     1981 cagctggtcg taggcgtccc ccagcccccg ctcctcgttc cggcgctcgt ccggggcgaa
     2041 ttggtcacgc caccagcgga ggttgccgcc agagacgaac cccgggttct cgaggagcca
     2101 cgcatcgggg tccgcgtgcg gatggcactc caccaacatc gtcggatcct cgcgcggctc
     2161 ggcgctcgcg gcgcacaccg gctcggcggt tccgacgacg tcgcacacct caccgggctc
     2221 gaacacgccg gctccgagcg tggccgccat ctcgtcgcca cagccgatcg cgacgacggt
     2281 ggccacagaa agg
//
LOCUS       DASUVE010000263         2207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_696731, whole genome shotgun sequence.
ACCESSION   DASUVE010000263 DASUVE010000000
VERSION     DASUVE010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696731"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VF036_05685"
     CDS             complement(<1..277)
                     /locus_tag="VF036_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding oxidoreductase"
                     /protein_id="HEX6264751.1"
                     /translation="MRAPGRALKGAFAGLVAAGLAIGVAQLVAGLLDPHTSPILTVGQ
                     ATIDLTPEGVKAFAIRTFGEQDKTVLLGGIALVLGLFAVGLGIWSLDR"
     gene            complement(435..1634)
                     /locus_tag="VF036_05690"
     CDS             complement(435..1634)
                     /locus_tag="VF036_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6264752.1"
                     /translation="MDGISERDVAERAGVEPGYVTRLVDVGVLTPADDGAFTEGDVRR
                     ARLYQGLERTGLPLEAIREALDAGELSFGWLDNPLYDLIAPLSQRSFQDVASDTGVPL
                     ELLRVLREAIGFALPEPDDRMREDELRVVPLIEKLVSARFPTSVLERLLRVYGDGLRR
                     MAEVEADWWHRYVVLPALASGMTDAEMHEATLGFGEETTPLLEQAMLAMYHAHQEHTW
                     VENLIGEIEDALDRTGLRSRLVRPPAICFLDITGYTRLTEERGDEAAAELATRLTPLV
                     QRSAERHDGKVVKWLGDGVMFHFREPNGGVVAALEMVDAIAAAGLPPAHVGLDTGPVV
                     FQGGDYFGRTVNLAARIGEQAAPGQVLVTQEIVDRVVPDGVAFEPVGAVELKGVSEPV
                     LLHSVIR"
     gene            complement(1684..1899)
                     /locus_tag="VF036_05695"
     CDS             complement(1684..1899)
                     /locus_tag="VF036_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264753.1"
                     /translation="MGGPAGFAFSQRGGTVVITHRGKKAVTLRGSVAARFLEDVERGD
                     PQQVMARVTGDYKRGNERTARAHPCNR"
     gene            1963..>2207
                     /locus_tag="VF036_05700"
     CDS             1963..>2207
                     /locus_tag="VF036_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264754.1"
                     /translation="MELTIEDVAGRAGVDVAYVRRLIDLGVVAPEGVGYREQDAHVTA
                     LLHMWEEAGLTAGSILTATASGQLSLDFLESPGWELPP"
BASE COUNT          330 a          795 c          741 g          341 t
ORIGIN      
        1 tccgatccag agaccagatc ccgaggccga cggcgaacag cccgagcacc agcgcgatcc
       61 cccccaggag gaccgtcttg tcctgctcgc cgaatgtcct gatggcgaat gccttgacgc
      121 cttcgggcgt caggtcgatc gtggcttgcc cgaccgtcag gatcggcgac gtgtgcggat
      181 cgaggagtcc ggccacgagc tgggcgacgc cgatcgccag gcccgccgcg acgaggcccg
      241 cgaacgcgcc cttgagggcc ctaccgggtg cgcgcacatg aacgagtctt cgggagttgc
      301 cttaccgatc cattacacgc cggcttcgcc tctcttaact ctcgcccagg tcgatccatc
      361 ggcccgttcg cggtagctca agagcttcga gcgacacaca acgttcgaac cgcgccagcc
      421 acccggtttc gtggtcaccg tatgacggag tgaaggagaa ccggttcgga cacgcccttc
      481 agctcgaccg cgccgacggg ctcgaaggcg accccgtccg ggaccacgcg atccacgatc
      541 tcctgcgtga cgagcacttg gcccggcgcc gcttgctccc cgatcctcgc cgcgaggttg
      601 accgttcggc cgaagtagtc gcccccttgg aagaccaccg ggccggtgtc gaggccgacg
      661 tgcgcgggcg gcagccccgc cgccgcgatc gcgtcgacca tctccaacgc ggcgacgacc
      721 cctccgttcg gctcccggaa gtggaacatc acgccgtcgc ccagccactt cacgaccttg
      781 ccgtcgtgcc gctcggccga ccgctggacg agcggcgtca accgcgtcgc cagctccgcc
      841 gccgcctcgt cacctcgctc ctccgtcagc cgcgtgtagc ccgtgatgtc caggaagcag
      901 atcgccgggg gtctgaccag ccggctgcgg agcccggtgc ggtcgagcgc gtcctcgatc
      961 tcgccgatca ggttctccac ccacgtgtgc tcctgatgcg cgtggtacat cgcgagcatc
     1021 gcctgctcga ggaggggcgt ggtctcctcg ccgaacccga gcgtcgcctc gtgcatctcc
     1081 gcgtcggtca tgccggaagc cagggcgggg agcacgacgt accggtgcca ccagtccgcc
     1141 tccacctcgg ccatccgtcg gagaccgtcg ccgtagacgc ggagcaaccg ctcgaggacg
     1201 gacgtgggga atcgggccga gacgagtttc tcgatcaacg ggacgacccg gagctcgtcc
     1261 tcgcgcatcc ggtcgtccgg ctcgggcagg gcgaagccga tcgcctcgcg gaggaccctg
     1321 aggagctcca gcgggacgcc ggtgtccgag gcgacgtcct ggaacgatcg ttggctgagc
     1381 ggggcgatca ggtcgtagag cgggttgtcg agccacccga acgagagctc cccggcgtcg
     1441 agcgcctcgc ggatcgcttc gagcggcaga cccgtgcgtt cgaggccctg gtacagccga
     1501 gcgcgcctga cgtcgccctc cgtgaaggcg ccgtcatcgg cgggcgtgag gacacctacg
     1561 tcgacgagtc tggtcacgta tccgggttcg acgcccgccc gctccgcgac gtctcgttcc
     1621 gagatcccgt ccacgcacaa aacctaaccg tggatccttc cggtgagcac ggcggtggcg
     1681 gcgtcagcgg ttgcacgggt gggcgcgcgc ggtccgttcg ttgcctcgct tgtagtcgcc
     1741 ggtcacgcgc gccatcacct gctgcggatc gccccgctcg acgtcttcca ggaagcgcgc
     1801 ggcgacggaa ccgcggagcg tgaccgcctt cttcccacga tgcgtgatca cgacggtgcc
     1861 cccacgttgc gagaacgcga acccggcagg gccacccatg gggcgaggat gccacggtcg
     1921 atgtggcttc cgccatcatg gtgcggaggc agggaggcgt cggtggagct cacgatcgag
     1981 gacgttgccg ggcgcgcggg ggtcgacgtc gcgtacgtcc gccggctgat cgacctgggt
     2041 gtcgtcgcgc cggaaggcgt cggctaccgc gaacaggacg cgcacgtcac cgctctcctt
     2101 cacatgtggg aggaggccgg gctcaccgcc gggtcgatcc tcaccgcgac ggcgtccggg
     2161 cagctgtccc tggacttcct cgagtcgccc ggatgggagc tcccccc
//
LOCUS       DASUVE010000264         4713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1637449, whole genome shotgun sequence.
ACCESSION   DASUVE010000264 DASUVE010000000
VERSION     DASUVE010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637449"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VF036_05705"
     CDS             <1..456
                     /locus_tag="VF036_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6264755.1"
                     /translation="SCDERGAMTSEEGFRVPDVCRIVGISYRQLDYWARTELVTPSIR
                     DARGSGTQRLYSFQDLVSLKVIKKLLDTGVSLQRVRKAVEHLHSMKRPTHRVTLMSDG
                     RGVYEAHSAEAVIDLLQKGQGVFAIALDQVWSDLEGTLSKRRRAAAGGS"
     gene            453..839
                     /locus_tag="VF036_05710"
     CDS             453..839
                     /locus_tag="VF036_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264756.1"
                     /translation="MSARAVTPVAPTLESLPKSETVGFAHASERQFAQLLDFYGIVWE
                     YEPTSFDIEWDREGNVVRRFTPDFFLPEFDLYIEITTLNQKLVTRKNRKVRQLRERYP
                     EIRCKVFYQRDYLSLVTKYGLEDRSG"
     gene            868..2190
                     /gene="gcvPA"
                     /locus_tag="VF036_05715"
     CDS             868..2190
                     /gene="gcvPA"
                     /locus_tag="VF036_05715"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX6264757.1"
                     /translation="MKFAPHTDAEIDEMLSDIGIDSLDELFEQIPPSVRLEGSLGIPD
                     GASELEIVEDLRALAARDRHLDELVCFAGAGAYDHFIPAVVWALAARSEFSTSYTPYQ
                     PELSQGVLQVLFEFQSMVCELTGMDVSNASLYDGATALVEAVHMARGGERTRILVSEG
                     VDPRLVETLRTYGKGSGYEPEIFPSEHGRGGAPAVGDDVAAVVVQHPNVLGILEPASE
                     LFSAAREGGARAIQVFDPLSLGVLAPPGELGADIAVAEGQSLGNHLAFGGPYLGLIAA
                     RMSDVRRMPGRIVGETVDVDGTPGYVLTLQAREQHIRREKANSNICTNQTLMAVAATI
                     YLGWLGPDGLVEVGRRCAAKAAYAADRLTEIEGVSLLHPSVPFFKEFALRVPVAADRV
                     VAALVERGFLAGVPLLREGDDVMLVAVTERRSKEQIDAFAEAMGEALA"
     gene            2241..3662
                     /gene="gcvPB"
                     /locus_tag="VF036_05720"
     CDS             2241..3662
                     /gene="gcvPB"
                     /locus_tag="VF036_05720"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014196335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX6264758.1"
                     /translation="MLDRSVPGRRAWSLPPLDVAEAPVPEEHARGRRPGLPEVGEVDL
                     VRHYTGLAQMNYGLDTGMYPLGSCTMKYNPKVAESIAALPGFQRVHPLQPDSTTQGVL
                     ELLWRLERALCEITGMDQATLQPPAGACGEMTGLLLMRAFHEDRGDARRRVIIPDSAH
                     GTNPASVRLAGFEAVPVRSDARGLVDAGALAKLVDDSVAGLMLTNPNTLGLFEEEIEE
                     IAATIHGVGGLLYYDGANLNAILGRCRPGDMGFDIVHINTHKTFATPHGGGGPGAGPV
                     GVKDELAAFLPAPTVRRNADGGFSLDPGGERSIGRMHGFHGNVGILVRAYAYVFLHGA
                     DGLREVSELAALNANYLARLVEEEFPLAHPGMRPMHEFVATAKRLRDATGIRAMDVAK
                     RMIDLGFHPSTVYFPLVVEEALMVEPTETESKATLEAFGEALRRTAEEARERPEFLRE
                     APVTTPVRRLDEARAARALKLRW"
     gene            3801..4250
                     /locus_tag="VF036_05725"
     CDS             3801..4250
                     /locus_tag="VF036_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX6264759.1"
                     /translation="MRRGLVGAKVLGLTLVLTVVFGALAPSARAAWTPRRDMLGWMNT
                     ARSDHGQVVLDPGWRLRAIADAHSRRMASAGRIFHTTSFGSTLTSVSWNVAGENVGAG
                     GSMRALYEAFMKSDPHRDNILGRGFRRVGVGVYAHGGFLWVTLIFVG"
     gene            complement(4318..>4713)
                     /locus_tag="VF036_05730"
     CDS             complement(4318..>4713)
                     /locus_tag="VF036_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264760.1"
                     /translation="GTTVTYTYVVTNTGDTTLYDISVDDDILGHIGDIAVLEPGESAT
                     LTKDFVVGDVPVTNVGTATGEDILGRSVSSDDDAVVTPIAGATPPNPPTPFTGSDAGR
                     LGIISMVLFGIGVTLVATTRKRRPEREAA"
BASE COUNT          711 a         1589 c         1659 g          754 t
ORIGIN      
        1 agctgtgatg agcggggagc gatgacgagc gaagaggggt tccgggtacc ggacgtctgt
       61 cggatcgtgg gtatcagcta tcgccagctc gactattggg ccaggacgga gctcgtgacg
      121 ccctcgatcc gcgacgcgcg agggtcgggg acgcagcggc tgtactcgtt ccaggacctc
      181 gtctccttga aggtcatcaa gaagctcctg gacaccgggg tgtccctcca gcgggtccgc
      241 aaggccgtcg agcacctgca ttcgatgaag cgacccacgc accgagtcac cttgatgtcg
      301 gacggccgtg gggtctacga ggcccactcg gcggaggccg tgatcgacct cctccagaag
      361 ggacagggcg tcttcgccat cgccctcgac caggtctgga gcgatctgga ggggaccctc
      421 tcgaaacgca gacgggcggc cgcaggaggg tcgtgagcgc gcgagccgtg accccggtcg
      481 cacccacgtt ggagtccctc cccaagagcg aaacggtcgg tttcgcccac gcttccgagc
      541 gccagttcgc gcagctgctc gacttctacg gcatcgtctg ggagtacgag ccgacatcgt
      601 tcgacatcga atgggaccgt gagggcaacg tggtgcgccg cttcaccccg gacttcttcc
      661 tcccggagtt cgacctctac atcgagatca ccacgctgaa ccagaagctc gtcaccagga
      721 agaaccggaa ggtccgtcag ctgcgcgagc gctatccgga gatccggtgc aaggtcttct
      781 atcagcgcga ctacctctcg ctcgtcacga agtacgggct cgaggaccgc tccggctgag
      841 ctgcggtctg cccgtacact gctcgccgtg aagttcgccc cgcatacgga tgccgagatc
      901 gacgagatgc tctcggacat cggcatcgac tccctcgacg agttgttcga gcagatccct
      961 ccctcggtcc gtctcgaggg gtcgctcggg atcccggacg gcgcctcgga gctcgagatc
     1021 gtcgaggacc tccgcgcgct cgccgcacgc gatcggcacc tggacgagct ggtgtgcttc
     1081 gccggcgccg gcgcctacga ccacttcatc cccgccgtgg tgtgggcgct cgccgcccga
     1141 tcggagttct cgacctcgta cacgccgtat caaccggagc tctcgcaagg cgtgctccag
     1201 gtcctgttcg agttccaatc gatggtgtgc gagctcaccg ggatggacgt gtcgaatgcc
     1261 tccctctacg acggcgcgac ggcgctcgtg gaggccgtac acatggccag agggggcgaa
     1321 cggacccgga tcctcgtctc ggagggggtg gacccgaggc tggtcgagac cctccgaacg
     1381 tacgggaagg ggtccgggta cgagccggag atcttcccat ccgagcatgg ccggggaggt
     1441 gcgccggccg tcggagacga cgtcgcggcc gtcgtcgttc agcaccccaa cgtgctcggg
     1501 atcctcgagc cggcctccga gctcttctcc gcggccaggg agggcggcgc cagagcgatc
     1561 caggtcttcg atccgctctc cctgggggtg ctggctccgc cgggggagct cggtgcggac
     1621 atcgccgtcg ccgagggcca gtccctcggg aaccatctcg ctttcggcgg cccgtacctg
     1681 gggctcatcg ccgccaggat gtcggacgtg cgccggatgc ccggcaggat cgtgggggag
     1741 acggtcgacg tcgacggcac gcccggatac gtgctcaccc tgcaagcgcg agagcagcac
     1801 atccgtcgtg agaaagccaa ctcgaacatc tgcacgaacc agaccctgat ggcggtggcg
     1861 gccacgatct acctcgggtg gctcggtccg gatggcctcg tcgaggtcgg gcgtcgatgc
     1921 gccgcgaagg cagcctacgc ggctgaccgg ctcaccgaga tcgagggcgt ctcactcctg
     1981 catccctccg tgccgttctt caaggagttc gcgctccggg ttcccgtcgc tgcggaccgc
     2041 gtcgtcgccg ctctcgtgga gcgcgggttc ctcgcgggcg tccccctgct gcgggaggga
     2101 gacgacgtca tgctcgtggc cgtcacggag cggcggagca aggagcagat cgatgctttc
     2161 gccgaggcga tgggggaggc gctcgcgtga acgcggtggg ggcgccgatc cgccggcagg
     2221 gtcccggagc cgacggcacg atcctcgacc gctccgttcc cggccgccgc gcctggagcc
     2281 tgccgcccct ggacgttgcc gaggctcccg tgccggagga gcacgcccgc ggacgtcgtc
     2341 ccgggctgcc cgaggtgggg gaggtggacc tcgtccgcca ctacacgggc ctggcgcaga
     2401 tgaactacgg cctcgatacg gggatgtacc ccctcggctc gtgcacgatg aagtacaacc
     2461 cgaaggtcgc cgagtccatc gccgccctcc cgggtttcca gcgcgtccac cccctccagc
     2521 cggacagcac cacgcaaggc gtgctggagc tcctgtggcg cctggagcgc gctctgtgcg
     2581 agatcaccgg gatggaccag gcgacgctgc agccgccggc cggtgcgtgc ggcgagatga
     2641 ccggtctcct cctgatgcgc gcgttccacg aggatcgcgg cgacgcgagg cgtcgcgtga
     2701 tcatcccgga ttccgcccac gggactaacc ccgccagcgt gcggctcgcg gggttcgagg
     2761 ccgtgccggt ccgctccgac gcccgtggtc tggtcgacgc cggggcgctc gcgaagctcg
     2821 tggacgactc cgtggccggt ctgatgctca ccaacccgaa caccctcggt ctgttcgagg
     2881 aggagatcga ggagatcgcg gcgacgatcc acggtgtggg cgggctcctc tactacgacg
     2941 gggcgaacct gaacgcgatc ctcgggcggt gtcgtcccgg cgacatgggt ttcgacatcg
     3001 tccatatcaa cacccacaag acgttcgcga caccgcacgg aggaggtggc ccgggggcgg
     3061 gaccggtggg ggtgaaggac gagctggccg ccttcctgcc cgcaccgacc gtccggagga
     3121 acgcggatgg gggattctcg ctcgaccccg gtggggagcg ctcgatcggt cggatgcacg
     3181 ggttccacgg gaacgtcggg atcctggtcc gggcctacgc ctacgtgttc ctgcacggtg
     3241 ccgacgggct cagggaggtg agcgagctgg cggcgttgaa cgcgaactac ctcgctcgcc
     3301 tcgtggagga ggagttcccg ctcgcgcatc cgggcatgcg gccgatgcac gagttcgtgg
     3361 caaccgcgaa gcggctccga gacgcgaccg ggatcagggc catggatgtc gcgaaacgca
     3421 tgatcgacct ggggttccac ccgtcgacgg tgtacttccc tctcgtcgtg gaggaggccc
     3481 tgatggtgga gccgacggag acggagtcga aggcgaccct cgaggccttc ggcgaggcgc
     3541 tccgccggac ggcggaggag gctcgcgagc gacccgaatt cctgcgggag gcgcccgtga
     3601 ccacgcccgt gcgtcgcctc gacgaagctc gcgcggcccg agcgctgaag ctccgctggt
     3661 agctctggcg ccactacctc atcccgagta gcacctcctg gtcatcttca ggcagatccc
     3721 tctcaagacc ggccgcccgg tgccgatgtc ggtccccagg ggcggccatg gggctgcccg
     3781 ctgggcaagg aggcgaaccg atgagacggg gtctggtcgg ggccaaggtc ctgggactca
     3841 cgctcgtcct caccgtcgtg ttcggcgcgc tcgcgcccag cgcacgagcc gcctggacgc
     3901 ctcgccgcga catgctcggc tggatgaaca ccgcgcggag cgatcacgga caggtcgtct
     3961 tggaccctgg ctggcggctc cgggcgatcg cggacgcgca cagccggcgg atggcgtctg
     4021 ccgggcggat cttccatacc acctcgttcg gatccacgct cacctccgtg tcgtggaacg
     4081 tggcggggga gaacgtgggc gcgggcggca gcatgcgggc gctgtacgag gcgttcatga
     4141 agagcgaccc tcaccgggac aacatcctcg ggaggggatt ccgtcgcgtc ggcgtcgggg
     4201 tgtacgcgca cggcgggttc ctctgggtga cgctgatctt cgtcggctga ggccggagac
     4261 gcaggaaggg gcgggtcccg atgggacccc gccccttcct tccgtaccgc tcgactgtta
     4321 cgcggcctcg cgctccggcc ggcgcttccg ggtggtcgcg accaaggtca cgccgatccc
     4381 gaagagcacc atcgagatga tccccagccg tccggcgtcc gatcccgtga acggggtcgg
     4441 cgggttcggc ggggtcgcgc cggcgatcgg ggtcacgacg gcgtcgtcgt cggagctcac
     4501 cgaccggccg aggatgtcct cgccggtcgc cgtgccgacg ttggtgaccg gaacgtcacc
     4561 caccacgaag tccttcgtca gcgtggcgga ctccccgggc tcgagcaccg cgatgtcccc
     4621 gatgtgcccc aggatgtcgt cgtcgacgct gatgtcgtac agcgtggtgt cgcccgtgtt
     4681 cgtgacgacg taggtgtagg tcacggtcgt gcc
//
LOCUS       DASUVE010000265         2964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_320537, whole genome shotgun sequence.
ACCESSION   DASUVE010000265 DASUVE010000000
VERSION     DASUVE010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320537"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VF036_05735"
     CDS             <1..196
                     /locus_tag="VF036_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEX6264761.1"
                     /translation="LIRTKVSLIPDTVDPIRVIDIEGIDKQADGGTHVKATGEIGHVT
                     VVKTESKGKAFKRMRIQLEA"
     gene            complement(200..2134)
                     /locus_tag="VF036_05740"
     CDS             complement(200..2134)
                     /locus_tag="VF036_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEX6264762.1"
                     /translation="MRRLATALALAALILSMPGPAGAQDPANEASYGASVSAIDDLFT
                     PEIVRIEVGETVEWIMDGRSRHTVEADDGSWSSGNLDPGAEFDHTFDAEGTFPFFCRY
                     HGRPGLGMAGTVVVGDAPLPGGGVPGPDPAPAGPANTVHVPDDAPTIQDAVDRARPGG
                     LVLIDPGVYREAVVVTTPYLTIRGMDRNDTILEGNFELANGIHVIEADGVAVENLTAR
                     HFLLNGFNWSNVLGYRGSYLTASTNGDYGIYAFASRWGRFDHSYASGSPDAGFSIGAC
                     NPCDAVITDVLAEHNALGYSGTNAGGNLAIVNSEWRENLVGIVPNTLDSEPFAPQRDV
                     LIAGNHVHHNNSTTVDPKEYGYVPFGMGIVVAGGREDRIIGNLVEDQATYGIAMIPIV
                     DTNFWPSSGNEVRGNVVRRSGRADMALAAPSAGADCFGGNDAATSHPASVELLFPCSG
                     FRPFPGGGGSMGPTANVLSRFIESLDGEFPHGDWRTQPDPPVDLPDMPGDPAAAPPDP
                     AIPEEAVPARYRIRVVEDIRAVRGSRISQEVSLMGVPLIASSWWSLLLGLYGYILPFV
                     LYASWVAIAMWDLIRRDADAIPARAGWMAIVLLVPFVGPLLYLAFGGSSIPSQLRLVL
                     TAGGVIAYLFFFVIGLLVGG"
     gene            2159..>2964
                     /locus_tag="VF036_05745"
     CDS             2159..>2964
                     /locus_tag="VF036_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX6264763.1"
                     /translation="MPAERLSGLDASFLYLEKPGVHMHVAGLSIFGAREDGSLLTYDD
                     VERVVEARLRLAPRLRRKLLSVPGNLSRPLWIDDDRFDLDFHLRRASVPAPGGRFELE
                     RAVGRVLSRPLDRTKPLWELYVFEDLSEDRTAVLLKIHHAMADGIGNMMIASALFDLT
                     ADAPMGEPDGAWSAEPAPPTEELIRDAVEETILHPIQSLAHVARQPRIVTETLSATAD
                     ALRTVAGMGTAPRGPFDGRVGPNRRFATAERPFAVFRGIKESLGGTVNDVV"
BASE COUNT          481 a          998 c         1042 g          443 t
ORIGIN      
        1 cctgatccgc acgaaggtca gcctgatccc cgacaccgtc gacccgatcc gcgtgatcga
       61 catcgagggc atcgacaagc aggcggacgg cgggacgcac gtgaaagcga cgggggagat
      121 cggtcacgtc accgtcgtca agacggagtc gaagggcaag gccttcaagc ggatgcggat
      181 ccagctcgag gcgtgaggct catccgccga cgagcagccc gatcacgaag aagaagaggt
      241 acgcgatcac cccacccgcc gtgagcacga ggcgcagctg cgaggggatc gaggatccgc
      301 cgaacgcgag gtagagcagc ggcccgacga acgggacgag gagcacgatc gccatccatc
      361 ccgcgcgagc cgggatcgca tcggcatcac gcctgatcag gtcccacatc gcgatcgcca
      421 cccacgaggc gtagagcacg aacgggagga tgtagccgta cagcccgagg agcaagctcc
      481 accagctgga tgcgatgagc gggactccca tgaggctgac ctcctggctg atccgagatc
      541 cacggaccgc gcggatgtcc tccacgacgc ggatccgata acgggccgga acggcctcct
      601 ccgggatcgc cggatcggga ggggcggctg ccggatcgcc cggcatgtcc gggaggtcca
      661 ccggcggatc cggctgcgtg cgccagtccc cgtgcgggaa ctcgccgtcg agcgactcga
      721 tgaaccggga caggacgttc gccgttggcc ccatactccc gcctccgccc gggaagggcc
      781 ggaacccgct gcaggggaag aggagctcga cggacgccgg gtggctcgtg gcggcgtcgt
      841 tgcccccgaa gcagtcggcg cccgccgagg gcgctgcgag cgccatgtcc gccctgccgc
      901 tccggcgaac gacgttgccg cggacctcgt tgccgctcga gggccagaag ttcgtgtcca
      961 cgatcgggat catcgcgatg ccgtacgtcg cctggtcctc gacgaggttc ccgatgatcc
     1021 gatcctctcg cccgccggcg acgacgatcc ccatcccgaa cggcacgtac ccgtactcct
     1081 tcgggtcgac cgtggtcgag ttgttgtggt ggacgtggtt gcccgcgatc aggacgtcgc
     1141 gttgcggcgc gaacggctcg gagtcgagcg tgttgggcac gatcccgacg aggttctccc
     1201 gccactcgct gttcacgatc gcgaggttcc ctcccgcgtt ggtcccggag tagccgagcg
     1261 cgttgtgctc ggcgagcaca tcggtgatca ccgcgtcaca agggttgcat gcgccgatgg
     1321 agaacccggc gtcgggcgag ccgctcgcgt atgagtgatc gaaccgcccc cagcgtgatg
     1381 cgaacgcgta gatcccgtag tcaccgttcg tggaggccgt gaggtaggag cctcggtacc
     1441 ccaggacgtt cgaccagttg aagccgttca acaggaagtg ccgcgcggtg aggttctcca
     1501 cggcgacacc gtccgcctcg atcacgtgga tcccgttcgc gagctcgaag ttcccctcga
     1561 ggatcgtgtc gttgcggtcc atgccgcgga tcgtgaggta gggggtggtg acgaccaccg
     1621 cctcgcggta gacaccgggg tcgatcagga cgaggccgcc ggggcgggcg cggtcgaccg
     1681 cgtcctggat cgtcggggcg tcatcaggga catgcaccgt gttggcaggt cccgcgggag
     1741 cggggtcggg accggggact ccgccgcccg ggagcggcgc gtccccgacg acgacggtgc
     1801 cggccatgcc gaggccgggc cgtccgtggt atcgacagaa gaacgggaag gtcccttcgg
     1861 catcgaacgt gtggtcgaac tctgccccgg gatcgaggtt ccccgaagac catgagccgt
     1921 catcggcctc gacggtgtgc cgggaccggc cgtccatgat ccactcgacc gtctcgccga
     1981 cctcgatccg gacgatctcc ggtgtgaaca ggtcgtcgat cgcggagacc gacgcgccgt
     2041 acgatgcctc gttcgccgga tcctgcgccc ccgcgggccc cggcatcgac aggatgagcg
     2101 cggcgagcgc caaggcggtc gcgaggcgac gcatcgcggc ggaccctagc atgggcggat
     2161 gcccgcggaa cgattgtcgg ggctcgatgc gagcttcctc tacctcgaga agcccggcgt
     2221 gcacatgcac gtcgccggac tctcgatctt cggtgcgcgc gaggacggct cgctcctcac
     2281 ctacgacgac gtggagcggg tcgtggaggc tcggcttcgt ctagctcctc ggctgcgtcg
     2341 gaagctcctg tcggttcccg gcaacctgtc ccggcccctg tggatcgacg acgatcggtt
     2401 cgacctcgac ttccacctgc gccgggcatc ggtccctgcc ccgggcggac ggttcgagct
     2461 cgagcgggcc gtgggtcgcg tgctgtcccg cccgctcgac cgaacgaagc ccctgtggga
     2521 gctctacgtg ttcgaggacc tctcggagga tcggactgcg gtcctgctga agatccatca
     2581 cgcgatggcg gacgggatcg ggaacatgat gatcgcgtcg gctctgttcg atctgacagc
     2641 cgatgcgccg atgggcgagc cggacggggc atggtcggcc gaaccggcgc cgccgacgga
     2701 ggagctgatc cgtgacgcgg tcgaggagac gatcctccac ccgatccaga gcctcgccca
     2761 cgtcgcacgc cagccgcgca tcgtcaccga aaccctgtcc gcgaccgcgg acgcgctccg
     2821 caccgtcgcg gggatgggga cggcgcctcg cggcccgttc gacggaaggg tcggtcccaa
     2881 ccgccgcttc gcgacggccg agcgaccgtt cgcggtgttc cgggggatca aggagtcgct
     2941 cggcggcacc gtgaacgatg tcgt
//
LOCUS       DASUVE010000266         6735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_131907, whole genome shotgun sequence.
ACCESSION   DASUVE010000266 DASUVE010000000
VERSION     DASUVE010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131907"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VF036_05750"
     CDS             complement(<1..802)
                     /locus_tag="VF036_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX6264764.1"
                     /translation="MADNQLAERRVLRQTEGFFDGKSPGVTLLLGAAFLAVVGVMDYL
                     LGPRLSVALFYLMPIGLVTWNLGLRWGAVTVALATAAALWSDLLYPQYDLLPWWNAIF
                     RFAAFLSVAVLLGTLRRIIAAQWDHLDREAAVTSGLRELNEVKDTLLHAVSHDLKGPL
                     AGILGSVQTLHRAEQIGLTEDERSTMLDMIEQGGKKMNRLVDDLLDLDRLDRGHIQPE
                     RAPTDVSELARRVARELPGMESHPIRVEASPVLVDVDAAKVERVIENLL"
     gene            complement(896..1507)
                     /gene="rdgB"
                     /locus_tag="VF036_05755"
     CDS             complement(896..1507)
                     /gene="rdgB"
                     /locus_tag="VF036_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX6264765.1"
                     /translation="MALPPRIAIASHNAHKLREIARICADWPVAWLTVENHEGPWPEV
                     EETGSTYLENARLKAEAGAAALGLPALADDSGIEVDALGGRPGPRSARFAGEDATDER
                     NLQELIRALKGVPGSGRTARYRCLAVLASPDGGDVSAEGICEGVLLTKPRGSRGFGYD
                     PIFVPAGWDHTMAELTDEEKDRISHRGRALRALRDVLEAAEPS"
     gene            complement(1509..2231)
                     /gene="rph"
                     /locus_tag="VF036_05760"
     CDS             complement(1509..2231)
                     /gene="rph"
                     /locus_tag="VF036_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX6264766.1"
                     /translation="MSARPDGRAPGELRPVSYELGYQEWAAGSVLFSMGKTRVLCAAS
                     VSTEAPRWLRGTGKGWVTGEYSMLPASTNERSPREVNRGRPGGRTQEIQRLIGRSLRA
                     VTDLGRLPDRTITIDCDVLQADAGTRTAAITGGYLALALALRGLSDAGLVPDDLLIDS
                     VAAVSVGLVDGEARLDLCYEEDAAAEVDCNVVMTGSGTLVEVQGTAEGAPFDRVQLDD
                     LLDLASAGVAELTKVQHDALDR"
     gene            complement(2228..2980)
                     /locus_tag="VF036_05765"
     CDS             complement(2228..2980)
                     /locus_tag="VF036_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6264767.1"
                     /translation="MELTVLGAQGTWPGAGGENCGYLVTEDGYHLQVDAGTGTFARLQ
                     QVVGVDEVGAMLITHGHPDHYVDIIPTFYARHYGRLGHPGLPFWSPGGFTDIAASLVS
                     ENGRNVMAEAFDFQTAVEGEVFELGPFRITPFEMQHIGVLSLGYRIESGGVVLAYTGD
                     TGPCEAAIELGRDADLFLCEATYQNASKLYPFHLSAAQAGEVASAAGVDHLVLTHLTP
                     ELDHRVSIREAAETFGGHLDIAVPGMLLEVGR"
     gene            complement(2971..3783)
                     /gene="murI"
                     /locus_tag="VF036_05770"
     CDS             complement(2971..3783)
                     /gene="murI"
                     /locus_tag="VF036_05770"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX6264768.1"
                     /translation="MSDPRPIGVFDSGVGGLTVARAIIDLLPHEPLLYVGDSARFPYG
                     SQPVERIRTYALEIAGYLVRRDVKVLVIACNSIEVSAIGDIAVTADIPVVGVVNPGSR
                     AAIRATRNGIVGVIGTEATIATGAYQRAMGSAVTLHAAACPAFVDHVERGDTSSPALR
                     EAAQGYLEPLMDAGIDTLVLGCTHYPLLSGLLQLELGPDVVLVSSAEETAKDVYGELV
                     SRDLLAPEGGRPVHEFATTGDPARFQDLAELFLGPEVRDLGVRQVTVEGAWS"
     gene            complement(3785..4717)
                     /locus_tag="VF036_05775"
     CDS             complement(3785..4717)
                     /locus_tag="VF036_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX6264769.1"
                     /translation="MTRHDDILGAIGNTPLVGLPSMSPKAGVRLWAKLEGNNPTGSTK
                     DRIALAMVVAAEASGVLTPEKTILEPTSGNTGISLAMVARRKGYHLTVVLPDNASQER
                     LGLLRLFGAEIVFSDGDKGTNGSIEVARALAEDAAYYMPFQYGNPANPKAHEGTGREI
                     VADLPEVTHFVAGMGTGGTLTGAGRALHAHDPSIRVVAAEPELGDLVYGLRSLDEGFI
                     PPIFDPSEVNRKFLVRSDDAVRATRELTSREGIFAGISSGAVIHVAQRIAAEVDGGDV
                     VCLLADGGWKYLSTEAWAEDLATAEKQVSESLWW"
     gene            4802..6157
                     /locus_tag="VF036_05780"
     CDS             4802..6157
                     /locus_tag="VF036_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6264770.1"
                     /translation="MDPETRYAKTVDGVHIAFQVRGDGPSELVYINGFTANFEVELED
                     RASAAFVDRLASFTRLILFDKRGTGLSDRQQTPDLDMRADDLRAVLDAVGSTQAALLG
                     HSEGGALGAFFAATHPDRVSALILYGSAARYAWAPDYPLGMPHEEYLAEREEIERTWG
                     TMEHARRWMEDETPSFVQDEEAVRWWAKAERFSASPAVAVEFEDIWYATDVRGVLGAI
                     QAPTLVICRPEADTAERSRYLADRIPGARYGELSGKDYPLVAGDVDEAFGAIHGFLTS
                     ISAEAAEIDRVLATVLFTDIVGSTERATSMTGEAWKALVERHHEVVRALLGRYRGREV
                     DTAGDGFFATFDGPARAVRCAQAIVEAVKPLGIEIRAGLHTGEAEVIDEKVGGVAVSI
                     GARVGAAARPSEVLVSSTVKDLVAGSGLEFEDRGEHELKGVPGRWFLFRAVGTTGGTA
                     S"
     gene            complement(6154..6429)
                     /locus_tag="VF036_05785"
     CDS             complement(6154..6429)
                     /locus_tag="VF036_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX6264771.1"
                     /translation="MSVQVKLPTILRKHAGGEPKVSAEGSTLRDVLDDLETRYPGITK
                     NVRSDDGGLHRFINVYLNDEDVRYLGSLETEVGEGDTVSILPAVAGG"
     gene            complement(6444..>6735)
                     /locus_tag="VF036_05790"
     CDS             complement(6444..>6735)
                     /locus_tag="VF036_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX6264772.1"
                     /translation="FPQPPPPGTVPSCAEGGVFGVLPGIVGCIQATEAVKLITGIGEP
                     LIGRLLIFDALPMEFTTVKIRWDADCPVCGKEPTITELIDYEQFCGVPARPD"
BASE COUNT         1029 a         2345 c         2354 g         1007 t
ORIGIN      
        1 cgagcaggtt ctcgatcaca cgctcgacct tcgcggcgtc gacgtcgacg agcacgggcg
       61 acgcctcgac ccggatcggg tgcgactcca tccccgggag ctcacgggcg acccggcggg
      121 ccagttcgct cacgtcggtc ggtgcgcgct cgggctggat gtgcccgcga tccagccggt
      181 cgaggtcgag caggtcgtcg acgagacggt tcatcttctt gccgccctgc tcgatcatgt
      241 ccagcatcgt cgagcgctcg tcctcggtca gcccgatctg ttcggcccgg tgcagggtct
      301 ggaccgaccc gaggatgccg gcgagcggac ccttcaggtc gtgcgagacg gcgtgcaaga
      361 gggtgtcctt gacctcgttc agctcgcgga ggccactggt cacggcggcc tcccggtcga
      421 ggtggtccca ctgcgcggcg atgatccggc gcagggtccc cagcaggacg gcgaccgaga
      481 ggaacgccgc gaaccggaag atcgcgttcc accacgggag caggtcgtac tgcgggtaga
      541 ggaggtcgct ccagagcgcg gcagcggtcg ccagcgcgac ggtcacggcc ccccaccgca
      601 agccgaggtt ccacgtcacg agcccgatcg gcatcaggta gaacagcgcg accgacaggc
      661 gagggccgag gaggtagtcc atcacgccga cgaccgcgag gaacgcggcg ccgagcaaga
      721 gcgtgacacc cggggacttc ccgtcgaaga agccctcggt ctgtctcagg acacggcgct
      781 ctgccagctg gttgtccgcc acgaagaaaa cttcggccgc ccgcagggtt accttgagtc
      841 gggtatcccg accgcgagtg cggcccttct cgggagcagc tcgcagcact ctcgttcacg
      901 agggctcggc ggcctcgaga acgtctcgga gcgcgcggag ggcccggccc cgatggctga
      961 tccggtcctt ctcctcatcc gtgagctcgg ccatggtgtg gtcccagccc gccgggacga
     1021 atatcgggtc gtagccgaag ccccggctcc cccgaggctt cgtgagcaag accccctcgc
     1081 agatcccttc ggcggagacg tctccgccgt ccggtgaggc gaggacggcc aggcagcgat
     1141 agcgcgccgt ccgcccgctc ccgggcaccc ccttgagcgc gcggatcagc tcctggaggt
     1201 tgcgctcgtc cgtggcatcc tcgccggcga accgtgccga ccgagggccg gggcggccgc
     1261 ccagcgcgtc cacctcgatg ccggagtcgt cggcgagcgc ggggagaccg agcgcggcgg
     1321 ccccggcctc tgccttcagg cgggcgttct ccagatacgt gctgcccgtc tcctccacct
     1381 cgggccaggg cccctcgtgg ttctcgaccg tgagccacgc gaccggccag tccgcgcaga
     1441 tcctggcgat ctcgcgcagc ttgtgggcgt tgtgggatgc gatcgcgatg cgggggggaa
     1501 gcgccacctc agcggtcgag agcgtcgtgc tggaccttcg tgagctcggc cacgcccgcc
     1561 gaggcgaggt cgaggaggtc gtccagctgc acccgatcga acggcgctcc ctcggcggtg
     1621 ccctggacct cgacgagggt ccccgagccg gtcatcacga cgttgcagtc gacctcggcg
     1681 gcggcgtcct cctcgtagca gaggtcgagc ctcgcttccc cgtcgacgag cccgacggac
     1741 acggcggcca cgctgtcgat caggaggtcg tcgggcacca gcccggcatc ggacagccct
     1801 cggagggcga gtgcgagagc gaggtaaccg ccagtgatcg cagccgttcg ggtgccggca
     1861 tcggcctgga ggacatcgca gtcgatcgtg atcgtccgat ccgggagccg gcccaggtcc
     1921 gtcacggctc ggagggaacg gccgatcagc cgctggatct cctgggtccg cccgccgggg
     1981 cgaccgcggt tcacctcccg tggcgaccgc tcgttcgtgg acgcggggag catcgagtac
     2041 tcgccggtca cccacccctt gccggtcccg cggagccagc ggggagcctc ggtggaaacg
     2101 gaggcggcgc acaggacgcg tgtcttgccc atcgagaaca ggacggaccc ggccgcccac
     2161 tcctggtacc cgagctcgta ggagaccggt cggagctcgc cgggtgcccg cccgtcgggg
     2221 cgtgcgctca tcggccgacc tcgaggagca tcccgggcac cgcgatatcg agatgccctc
     2281 cgaacgtctc ggccgcctcc cggatcgaga cccgatggtc gagctccggg gtgagatggg
     2341 tgagtacgag gtgatcgacg cctgcggcgg acgccacctc gcccgcctgc gccgccgaca
     2401 gatggaacgg atagagcttc gaggcgttct ggtacgtggc ctcgcacagg aacaggtccg
     2461 catcgcgccc gagctcgatc gccgcctcgc acgggcccgt gtcccccgtg tacgccagca
     2521 ccaccccacc ggactcgatc cggtacccga gcgagagcac gccgatgtgc tgcatctcga
     2581 acggagtgat ccggaacggc cccagctcga acacctcgcc ctccaccgcg gtctggaagt
     2641 cgaacgcctc ggccatcacg ttgcgcccgt tctcggagac gagcgacgcg gcgatgtcgg
     2701 tgaacccgcc gggcgaccag aacgggagtc ccgggtggcc gaggcgtccg tagtgccgcg
     2761 cgtagaacgt ggggatgatg tccacgtagt ggtcggggtg accgtgcgtg atcagcatcg
     2821 cgcccacctc gtcgacgccg accacctgct gcagccgggc gaacgtcccc gtcccggcgt
     2881 cgacctgaag gtggtagccg tcctcggtga cgaggtagcc acagttctcc ccgccggcgc
     2941 ccggccaggt tccctgcgcg ccgaggaccg tcagctccac gcgccctcca cggtcacctg
     3001 gcgcacgccg aggtcccgga cctccgggcc gaggaacagc tccgccagat cctggaaccg
     3061 ggccggatcg cccgtggtcg cgaactcgtg cacggggcgt ccgccctcgg gtgcgagcag
     3121 atcacgggac acgagctcac cgtacacgtc cttcgcggtc tcctccgccg acgacaccaa
     3181 gacgacgtcg ggcccgagct cgagctggag cagacccgag agcagcgggt aatgggtgca
     3241 cccgagcacg agcgtgtcga tcccggcgtc catgagcggc tcaagatacc cctgcgcggc
     3301 ctcccgcaac gcgggcgagc tggtgtcgcc tcgctcgacg tgatccacga aggcggggca
     3361 cgccgcggcg tgcagcgtca ccgcggatcc catcgcccgc tggtaggccc ccgtcgcgat
     3421 cgtggcctcc gtcccgatca ccccgacgat gccgttgcga gtggctcgga tcgccgcccg
     3481 cgacccgggg ttcaccacgc ccaccacggg gatgtccgcc gtcaccgcga tgtcgccgat
     3541 cgccgagacc tcgatcgagt tgcaggcgat cacgagcacc ttcacgtccc ggcggacgag
     3601 gtacccggcg atctccaggg cgtacgtgcg gatccgttcc accggctggg agccgtacgg
     3661 gaagcgcgcg gagtccccta cgtagagcag gggctcgtgc ggcaggagat cgatgatcgc
     3721 gcgcgcgacc gtgagcccgc ccacgccgga gtcgaacacg ccgatcggac gcgggtcgct
     3781 cacctcacca ccacagggac tcggagacct gcttctccgc ggtcgcgagg tcctcggccc
     3841 aggcctcggt cgacaggtac ttccacccgc cgtccgcgag gaggcagacc acgtcgcctc
     3901 cgtccacctc ggcggcgatc cgctgggcca cgtggatcac ggcgccggac gagatgcccg
     3961 cgaagatgcc ctcccgcgac gtcagctcgc gcgtggcccg gaccgcgtcg tcggagcgca
     4021 cgaggaactt ccggttcacc tcggacggat cgaagatcgg cgggatgaac ccctcgtcga
     4081 gggatcgcag gccgtagacg agatcgccga gctccggctc cgcggcgacg acccggatcg
     4141 acggatcgtg cgcgtgcagc gcccgaccgg cgccggtgag cgtgccaccg gttcccatcc
     4201 cggcgacgaa gtgcgtcacc tcggggaggt cggcgacgat ctcgcgcccg gtcccttcgt
     4261 gtgccttggg gttcgccggg ttcccgtact ggaacggcat gtagtacgcg gcatcctcgg
     4321 cgagggcgcg cgccacctcg atcgagccgt tcgtgccctt gtcgccgtcg gagaagacga
     4381 tctcggcgcc gaacaaccgg aggagaccga gccgctcctg gctcgcgttg tcggggagga
     4441 ccacggtgag gtgatagccc ttcctgcgag cgaccatcgc gagcgagatc cccgtgttcc
     4501 cggaggtcgg ctcgaggatg gtcttctcgg gcgtgaggac gccggacgcc tccgcggcca
     4561 cgaccatcgc gagcgcgatg cggtccttcg tggagccggt cgggttgttc ccctcgagct
     4621 tcgcccacag ccgcacgccg gccttcggtg acatcgaggg cagcccgacg agcggcgtgt
     4681 tcccgatcgc tcccaagatg tcgtcgtggc gcgtcacggc cggattatcc cagcggccac
     4741 gcgatggggt gaccaagggt cgacttgctc accgtgcgcg ccggtcggac actaggggtc
     4801 gatggatcct gagacccggt acgcgaagac ggtcgacggc gtgcacatcg cgttccaggt
     4861 ccgcggcgat ggtccgagcg agctcgtgta catcaacggg ttcacggcga acttcgaagt
     4921 cgagctcgag gatcgggcga gcgctgcgtt cgtcgaccgg ctcgcctcat tcacccgcct
     4981 gatcctcttc gacaagcgcg ggaccgggct ctccgaccgc cagcagacgc ccgacctcga
     5041 catgcgagcc gacgacctcc gggcggtcct cgacgcggtc ggttcgaccc aggcggccct
     5101 gctcggacac tcggagggtg gggcgctggg agccttcttc gccgccacgc accccgatcg
     5161 cgtgtcggca ctgatcctct acggatcagc tgcccgctac gcctgggcac ccgactaccc
     5221 actcgggatg cctcacgagg agtatctggc cgaacgggag gagatcgagc gtacgtgggg
     5281 aacgatggag cacgcgagga gatggatgga ggacgaaaca ccttcgttcg tccaggacga
     5341 ggaggccgtc cgctggtggg cgaaggccga gcggttctcc gcatcccctg cggttgccgt
     5401 cgagttcgag gacatctggt acgcgaccga cgtccggggc gtccttggcg cgatccaggc
     5461 gccgacgctc gtgatctgcc ggcctgaggc ggacacggcg gagcgctctc ggtacctcgc
     5521 cgatcggatc ccgggcgcgc ggtacggcga gctctcgggg aaggactacc ccctcgtggc
     5581 cggtgacgta gacgaggcgt tcggagcgat ccacgggttc ctcacgtcga tctccgccga
     5641 ggcggcggag atcgaccgcg tcctcgcgac ggtcctgttc acggacatcg tcggatcgac
     5701 cgagagagcg acgtcgatga cgggcgaggc gtggaaggct ttggtcgaac gccaccacga
     5761 ggtggtccgg gcgctcctcg gacgctatcg aggccgcgag gtcgacaccg ccggcgacgg
     5821 gttcttcgcg acgttcgacg gtcccgcaag ggcggtgcgc tgcgcgcagg cgatcgtcga
     5881 ggccgtgaaa ccgctgggca tcgagatccg ggccggtctg cacacaggtg aggcggaggt
     5941 catcgatgag aaggtcggcg gagtcgcggt gagcatcggc gctcgagtcg gcgccgccgc
     6001 acgtccctcc gaggtcctcg tttcgagcac ggtgaaggat ctcgtcgccg gttccggcct
     6061 cgagttcgag gatcgtgggg aacacgagct gaagggagtg cccggcagat ggttcctgtt
     6121 ccgcgccgtg ggaacgaccg gaggaacggc ttcctaaccc ccggcgacgg ccgggaggat
     6181 cgaaacggtg tcgccctccc ccacttcggt ctcgagggaa ccgaggtagc gcacgtcctc
     6241 gtcgttcagg tacacgttga tgaaccggtg aaggccgccg tcgtcggagc ggacgttctt
     6301 cgtgatgccg gggtatctcg tctcgaggtc gtcgagcacg tcgcggagcg tggagccctc
     6361 ggcgctcacc ttgggctctc ctcccgcgtg cttgcggagg atcgtgggca gcttcacctg
     6421 aacggacatc tggtcgtgac tccttagtcg ggccgggcgg gaacaccgca gaactgctcg
     6481 tagtcgatca gttccgtgat ggtgggctcc ttgccgcaca ccgggcagtc ggcgtcccat
     6541 cggatcttga cggtcgtgaa ctccatcggc agcgcatcga agatcagcag ccgtccgatc
     6601 agcggctcgc cgatgcccgt gatgagcttg acggcctcgg tcgcctggat gcatcccacg
     6661 atcccgggga gcacgccgaa cacgccgccc tccgcgcacg acgggacggt gcccggcggt
     6721 ggcggctgag gaaac
//
LOCUS       DASUVE010000267         2810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1639109, whole genome shotgun sequence.
ACCESSION   DASUVE010000267 DASUVE010000000
VERSION     DASUVE010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639109"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VF036_05795"
     CDS             complement(<1..807)
                     /locus_tag="VF036_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX6264773.1"
                     /translation="MANASLKRVLVGRPLASHKAEHQLLPRFLALPVFSSDPLSSNAY
                     ATEEMMLVLVTAGASALAFRIPIAVAIATVLIIVIVSYRQTVRAYPGGGGSYIVAHEN
                     LGTVPGLIAAAALLIDYVLTVSVSVVAGMLAITSAAPALAEHKVIFSIGIVALVTLAN
                     LRGVKEAGTLFAIPTYGFVAMVYVMIVTGFVRCLAGCPEAATANLPLEETATISLFLI
                     LRAFSSGATALTGVEAISNGVQAFRRPQSRNAASTLAMMGFMTVSMFIGIT"
     gene            complement(789..1046)
                     /locus_tag="VF036_05800"
     CDS             complement(789..1046)
                     /locus_tag="VF036_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264774.1"
                     /translation="MSTLSILAAGGGGDLLVGILIGGCLGVLIGPAFRAWQIRREWVD
                     ASREAGLTDQLLERIERDAESDAWPRSDLDDVTEPTWRTRR"
     gene            1221..2591
                     /locus_tag="VF036_05805"
     CDS             1221..2591
                     /locus_tag="VF036_05805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6264775.1"
                     /translation="MRALALPGLVTLGAVLPVDIPTTTAALCYVLAVVVATATGGLVA
                     GLVSSVASFLALNFFFTPPFHTFAVEETADLVALAVFLAVSVTVGTMFSRVIEQRARA
                     ERREREARLLNHLGTRLRSGVPTEDVLRSLAGSILELFDLARCEITSELSGRPVVAEN
                     PDRTTTDGHEEMVPMTVQDRELGRIRIVVSDGRPGMSADERGVLQTLASQMALAIDGL
                     RLGSEAEEARMEAETNRLRAALFSSVTHDLRTPLASITASVSTLLEDRSPLRDDQRRE
                     LLETIDQETGRLNRVIGNLMDLSRMRAGAVVPTKTPAAIEELIEGVLARSGQLLEQHD
                     LRLMLREDLPEIALDVVLIDQALTNIVENAARFTPPGRRITIGAARWRDGVQVRIADH
                     GPGIPREERDRVFEPFVRGEGSTGTGLGLAIARAIVEAHGGSIRIGEEPGGGTAVVLE
                     LPGGST"
     gene            2588..>2810
                     /locus_tag="VF036_05810"
     CDS             2588..>2810
                     /locus_tag="VF036_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019435158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6264776.1"
                     /translation="MREGRVLVVDDDPQILRAVRTSLQGHDYEVLTAGNGETALDLLP
                     DADVDLVVLDLGLPGIGGHEVIRRLRSWSD"
BASE COUNT          457 a          938 c          993 g          422 t
ORIGIN      
        1 cgtgatcccg atgaacatcg acacggtcat gaatcccatc atcgccagcg tgctcgcagc
       61 gttcctcgac tgcgggcgcc ggaacgcctg cacgccgttc gagatcgcct cgacaccggt
      121 gagggccgtc gcaccggagg agaaggctcg caggatcagg aagagactga tcgtcgccgt
      181 ttcctcgagc ggcaggttgg ccgtcgccgc ctccgggcat cctgcgaggc agcgcacgaa
      241 gccggtgacg atcatcacgt agaccatcgc cacgaaaccg taggtgggga tcgcgaagag
      301 ggtccctgcc tccttcaccc cccgcagatt cgccaaggtg acgagcgcga cgatcccgat
      361 ggagaagatc accttgtgct ctgcgagagc cggagctgcc gacgtgatgg caagcatccc
      421 cgcgacgacg gacaccgaca cggtgaggac gtaatcgatc aggagggcgg cggcggcgat
      481 gaggccaggt accgtcccca gattctcatg ggccacgata taggagccgc cgcctcccgg
      541 atacgctcga acggtctgac ggtaggacac gatcacgatg atgagtaccg tcgcgatcgc
      601 gaccgcgatc gggatccgga atgcgagtgc gctcgctccc gcggtgacga ggacgagcat
      661 catctcctcc gtcgcgtacg cgttcgacga cagcgggtcg gacgagaaga ctgggagcgc
      721 caggaacctc gggagcagct gatgctcggc cttatggctc gcgaggggac gtccgacgag
      781 gacgcgcttc agcgacgcgt tcgccatgtt ggttcggtca cgtcgtcgag gtccgagcga
      841 ggccaggcgt cggactcggc gtcgcgttcg atgcgctcga gtagctggtc cgtgaggcca
      901 gcctcgcgcg acgcgtcgac ccattcccgg cggatctgcc acgcgcggaa cgcgggaccg
      961 atcaggacgc ccagacagcc accgatcagg atcccgacga ggagatctcc gcctccacct
     1021 gccgccagta tcgagagcgt ggacatggcc caagcgtgga ccccgaggca tagcgacgga
     1081 gtagggaaga ggcgcgccgt cgtaagggtt ctgtaagggt tcgccggggc cagagagcac
     1141 cttgctaccc tccggcggga ggctcgggga tgatgcgcgt gagggatcgt cggaggtggc
     1201 cacgcttcct ccgtcgagcc gtgagggccc tcgctctgcc cgggctcgtc accctcggag
     1261 cggtgctccc ggtcgacatc ccgacgacga ccgccgccct gtgctacgtc ctcgcagtcg
     1321 tcgtcgccac cgccacgggc ggtctggtgg ccggactcgt ctcctcggtg gcctccttcc
     1381 tggcgctgaa cttcttcttc acacctccgt tccacacgtt cgcagtggag gagacggccg
     1441 atctcgtcgc cctcgccgtg ttcctcgcgg tgtcggtaac cgtcgggacg atgttctccc
     1501 gcgtcatcga gcaacgcgcg cgagcggagc ggcgcgagcg cgaggcgcgg ctcctcaatc
     1561 atctgggaac gcgcctccgc tccggggtgc cgacggaaga cgtgctccgg agcctcgctg
     1621 ggtcgatcct cgagctcttc gacctcgcgc ggtgcgagat caccagcgag ctctccggtc
     1681 ggccggtcgt ggccgagaac cccgaccgga cgaccaccga cgggcacgag gagatggtgc
     1741 ccatgaccgt gcaagaccgc gagctcgggc gcatccgcat cgtcgtaagc gacgggcgtc
     1801 ccgggatgtc ggccgacgag cgaggcgtcc tccagacgct cgcatcccag atggcgctcg
     1861 cgatcgacgg cctgcgactc ggatcggagg cggaggaggc gcggatggag gcggagacga
     1921 atcggctccg cgccgcgctc ttctcctccg tcacgcacga cctccggacc ccgctcgcat
     1981 cgatcaccgc atccgtctcc accctccttg aggaccggtc gccgctccgc gacgaccagc
     2041 ggcgagagct gctggagacg atcgatcagg agaccgggcg cctcaatcgg gtgatcggca
     2101 acctgatgga cctgtcgcgg atgcgggcgg gtgcggtggt cccgacgaag acccccgcgg
     2161 cgatcgagga gctgatcgag ggcgtgctgg ctcgctcggg ccagctcctc gagcagcacg
     2221 acctccgtct catgctccgg gaggaccttc ccgagatcgc gctcgacgtc gtgctgatcg
     2281 accaggcgct gacgaacatc gtggagaacg cggcccggtt cacaccgccc gggagacgga
     2341 tcacgatcgg ggcggcgcgg tggcgcgatg gggtccaggt gcggatcgcg gatcatggac
     2401 cggggatccc gcgggaggag cgtgaccgcg tattcgagcc gttcgtgcgc ggcgagggga
     2461 gcacggggac cggcctcggc ctggccatcg ctcgagcgat cgtcgaggcg catggcggtt
     2521 cgatccggat cggcgaggaa ccgggcggag ggacggccgt ggtgctcgag ctcccgggag
     2581 gttcgacgtg agggaggggc gggtgctcgt ggtcgacgac gacccccaga tcctgcgcgc
     2641 cgtgcgcacg agcctccagg gacacgacta cgaggtcctg acggccggca acggggagac
     2701 cgcgctggac ctgcttcccg acgcggacgt ggacctcgtc gttctggacc tgggcctgcc
     2761 gggcatcggc ggccacgagg tgatccgccg cctgcgctcg tggtccgacg
//
LOCUS       DASUVE010000268         5162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_885733, whole genome shotgun sequence.
ACCESSION   DASUVE010000268 DASUVE010000000
VERSION     DASUVE010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885733"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VF036_05815"
     CDS             <1..201
                     /locus_tag="VF036_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264777.1"
                     /translation="GMPETSTELFGGNDGTFTVEPGSVRLIAVDVEGRLLLIVGSVWP
                     EPRDQVERLVDGVIASVRFEGG"
     gene            complement(262..636)
                     /locus_tag="VF036_05820"
     CDS             complement(262..636)
                     /locus_tag="VF036_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6264778.1"
                     /translation="MRRRLQGTARGVIAALAALALAACGAEASGDVHPGPGGPDAVAV
                     VARDNTFEPAWLELPAGQDVEVEITNEGGTMHDFTVESLDLSTGPIEEGAVATASLTV
                     PAGETTFACSIHGGMDGVIVGT"
     gene            complement(656..886)
                     /locus_tag="VF036_05825"
     CDS             complement(656..886)
                     /locus_tag="VF036_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005272088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264779.1"
                     /translation="MATMLMEHGGWGGGPWFLLFPLLWGALLVTVLLVWRGRNRHWTG
                     GAEEILAERYAKGEISANELRQRREELRRNQR"
     gene            1090..2256
                     /locus_tag="VF036_05830"
     CDS             1090..2256
                     /locus_tag="VF036_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6264780.1"
                     /translation="MTAITEPDRGRSGIVFDAALAIGLAIFGVIGTYFASLQHQPLRR
                     PFDAGAAALVVIAALALVARRRNPLVVLGIVFGTTLLYFVIGYHDGPIWFPLIVAYTT
                     AAVRGHRLAAAIVGVSGFLLFPWLDFLLRDGPAPSLISLLALAAWLLVLFGAAEIVRI
                     RRERVAEVARMEKEESLRRAGEERLRIARELHDALGHHLSLISVQAGVALHVNEALPE
                     QARTSLAAIRDASKEALTELRSVLDILRQRGEAAPRFPGSTLARIEDLAAQATAAGLE
                     VSTEVVGEARPLPYGIEAAAFRIVQEGLTNVTRHAGRATATVRVAYGDRDLTVLVEDD
                     GRGAAAGASPHGSGKGIVGMRERVGALGGELEAGPRAEGGFRVRAVLPLEAAAT"
     gene            2253..3200
                     /locus_tag="VF036_05835"
     CDS             2253..3200
                     /locus_tag="VF036_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6264781.1"
                     /translation="MIRVLLADDQALVRAGFKALLDAQPDIEVVGEADDGEEALRLAA
                     VSEPDVVLMDIRMPGMDGLEATRRIVRDEALDRTRIVILTTFDEDEYVFEALRIGASG
                     FLVKDTDPEVLLQGVRAVARGDALLSPAVTRRVIGAIAERQMPTFGADAGAERILATV
                     MFSDIVSSTERAVEVGDRRWREELDRHDELVRCELVEHGGREIKTTGDGFLAVFDAPT
                     RAIRCAVAIREGLRGLGIDVRIGLHTGEVELRGEDVGGIAVNIGSRVADEGSAGDVLA
                     TSTVKDLVSGSRIGFADLGEHQLKGVGDRWRLFAVTDTG"
     gene            3208..3882
                     /locus_tag="VF036_05840"
     CDS             3208..3882
                     /locus_tag="VF036_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6264782.1"
                     /translation="MAAPIRVVLADDQALVRAGFRSLLDAQDDIDVVGEASSGDEAVG
                     LASELSPDVVLMDIRMPGKDGLAATRAIAEDERLAGVRIVVLTTFGLDEYVFEAIRSG
                     ASGFLVKDTEPDELVQAVRVVAGGDALLSPSVTRQLLAEFASRAKEPIRATGLEELTD
                     REREIVALVGEGLSNQEIADRLFVSPATAKTHVSRAMGKLGARDRAQLVVIAYESGLV
                     RPGWLG"
     gene            3941..4540
                     /locus_tag="VF036_05845"
     CDS             3941..4540
                     /locus_tag="VF036_05845"
                     /inference="COORDINATES: protein motif:HMM:NF022187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX6264783.1"
                     /translation="MKVGIIGTGKMGRGFAEALSTTHEVTVGSRDPERARTTAEKTGA
                     SGGATYADAANADVVILTVPWSAMDDTLAELGDVSGTVVVDVSYPYNRREREALEGRS
                     TAELIQDRLPAARVFKGWNHVHARHLTAPEVDGIASSVLIAGDDDEGKEAVFTLARDM
                     GFHPVDAGPLRATRDLEKLVGVMLFVKLGAIRVLSSGRS"
     gene            4537..>5162
                     /locus_tag="VF036_05850"
     CDS             4537..>5162
                     /locus_tag="VF036_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX6264784.1"
                     /translation="MTAELQTRVDAALRRAGRRPGLLVVSATDGQDTAFASRGPLPDP
                     PCAPERAVFEIGSITKVFTSLLLAIAAERGELGVDDPLVEHLPRRTRVPMRDGRPIRL
                     VDLATHRSGLPRLPPGFLLDALRHRDDPYARLSTEDILAALGKTRPRVAAGERFHYSN
                     YGAGVLGIALAQAAATDYEALVRERITRPLGLRDTVITMSEDHRGRLAA"
BASE COUNT          720 a         1808 c         1870 g          764 t
ORIGIN      
        1 gggatgccgg agacctcgac ggagttgttc ggcggcaacg acgggacctt caccgtcgag
       61 cccgggtcgg tgcggctcat cgccgtcgac gtcgaggggc ggttgctgct gatcgtcggg
      121 agcgtttggc cagagccgcg cgatcaggtc gaacggctcg tcgacggcgt gatcgcgtcg
      181 gttcgcttcg aaggaggctg atccctccga aggaggctga tccctccgtc accgtgtgat
      241 ggaggacggt gaccggggga ctcacgtccc cacgatcacc ccgtccatgc cgccgtggat
      301 cgagcaggcg aaggtggtct cgcccgcggg aaccgtgagc gaggcggtcg cgaccgcgcc
      361 ttcctcgatc ggacccgtgc tcagatcgag cgactcgacc gtgaagtcgt gcatcgtgcc
      421 gccctcgttg gtgatctcga cctcgacgtc ctggcccgcc ggcagctcga gccaagcggg
      481 ttcgaacgtg ttgtcacgtg ccaccacggc gacggcgtcg gggccccccg gtcccggatg
      541 gacgtcgccc gatgcctcgg cgccgcacgc ggccagggcc agggcggcca acgccgcgat
      601 cacgccacgg gctgtcccct gaagtctccg tctcatgacc ctctcccctc actcgctacc
      661 tttggttccg gcgcagctcc tcgcggcgct gccggagctc attcgccgag atctcgccct
      721 tggcgtagcg ctcggcgagg atctcctccg cgccgcccgt ccagtgccgg ttccgccccc
      781 gccacaccag gagcaccgtg accagcaggg cgccccacag gagcgggaac agcaggaacc
      841 acggcccgcc gccccatccg ccgtgctcca tcagcatcgt cgccatcgtt cggacacctc
      901 cccgaccggt cctcgttggc ttcgaccggc ctggacccag cctcgcctcc gctggtcggt
      961 gcgacgtcgg ctcccgggat ggttctgggc tacgccacgg ggcgtagccg cgatgacgac
     1021 ccggcgccga tgacggcctc gagcccccga cctacgctgc caccgtcgaa gcactggagg
     1081 aaccgacgga tgacggccat caccgaaccc gaccgcggtc gttccggcat cgtgttcgac
     1141 gccgcgctgg cgatcgggct ggcgatcttc ggcgtgatcg gcacgtactt cgcgtcgttg
     1201 cagcaccagc cgctccggcg gcccttcgat gcgggcgcgg ccgcactggt cgtgatcgcc
     1261 gcgctcgcgc tggtcgctcg ccgccggaac ccgctcgtgg tcctcgggat cgtgttcggg
     1321 acgacgctcc tgtacttcgt gatcggctac cacgatgggc cgatctggtt ccctttgatc
     1381 gtcgcgtaca ccacggccgc ggttcgcggg catcggctcg ctgccgcgat cgtcggcgtc
     1441 tcgggcttcc tcctgttccc ctggttggac ttcctcctcc gggacggacc agctccatcc
     1501 ctgatctcgc tcctggcgct cgccgcgtgg ctcctcgtgc tgttcggtgc ggccgagatc
     1561 gtcaggatcc ggcgcgagcg ggtcgccgag gtggcgagga tggaaaagga ggagtcgttg
     1621 cggcgcgccg gcgaggagcg gctccggatc gcgcgcgagc tccacgacgc gctcgggcac
     1681 cacctgtcgt tgatcagcgt gcaagcgggc gtcgcgttgc acgtgaacga ggcgctcccc
     1741 gagcaggccc gcacgtcgct cgccgcgatc cgcgacgcga gcaaggaggc gctcaccgag
     1801 ctgcgatccg tcctcgacat cctccggcag cgaggcgagg cggccccgcg cttcccgggc
     1861 tcgacgctcg cgcggatcga ggacctcgcc gcgcaggcaa cggccgccgg gctggaggtc
     1921 agtaccgagg tcgtcggcga ggcgcgcccc ctcccgtacg ggatcgaggc tgccgcgttc
     1981 cggatcgtcc aggaggggct cacgaacgtg acgcgccacg ccggccgcgc caccgcgacg
     2041 gtgcgggtcg cgtacggtga ccgggacctc accgtgctgg tcgaggacga cggccgaggt
     2101 gcggccgcgg gcgcctcgcc gcacgggagc ggcaagggga tcgtggggat gcgcgaacgg
     2161 gtgggagcgc tcggcgggga gctcgaggcc ggaccgagag ccgaaggcgg gttccgcgtg
     2221 cgcgccgtcc tcccgctgga ggccgcggcg acgtgatccg cgtccttctc gccgacgatc
     2281 aagcgctcgt ccgggccggc ttcaaggctc tcctcgatgc ccagcccgac atcgaggtcg
     2341 tgggggaggc cgacgacggt gaggaggccc tccggctggc cgccgtgtcg gagcccgacg
     2401 tggtgctgat ggacatccgg atgccaggga tggacgggct tgaggccacc aggcggatcg
     2461 tgcgggacga ggcgctcgat cgcacgcgga tcgtgatcct cacgacgttc gacgaagacg
     2521 agtacgtgtt cgaggccctc cggatcgggg cctcggggtt cctcgtgaag gacaccgacc
     2581 ccgaggtgct cctccaaggc gtgcgggccg tcgcccgcgg cgacgcgctc ctgtcacccg
     2641 ccgtcacgcg ccgggtgatc ggcgcgatcg ccgagcggca gatgccgacg ttcggggccg
     2701 acgccggcgc cgagcggatc ctggccaccg tgatgttcag cgacatcgtc tcgtcgaccg
     2761 aacgagcggt cgaggtcggg gaccgccggt ggcgcgagga gctcgaccgt cacgacgagc
     2821 tcgtccgctg tgagctcgtc gaacacggag gtcgcgagat caagacgaca ggcgacgggt
     2881 tcctcgcggt gttcgatgcc ccgacgcgcg cgatccgctg cgccgtcgcg atccgcgagg
     2941 ggctgcgcgg gctcggcatc gatgtccgga tcggtctcca caccggcgag gtcgagctcc
     3001 gcggcgaaga cgtcggcggg atcgccgtga acatcggctc ccgtgtggcg gacgaggggt
     3061 cggcgggcga tgtgctggcc acgagcacgg tgaaggatct cgtgagcggg tcgcggatcg
     3121 ggttcgccga tctcggtgag caccagctga agggcgtcgg ggatcggtgg cggctgttcg
     3181 ccgtcacgga cacggggtga tcccgggatg gccgctccga tccgtgtggt gctcgccgac
     3241 gatcaggccc tggtgcgcgc cgggttccgg tccctcctcg acgcccagga cgacatcgat
     3301 gtcgtcggcg aggcgagcag cggcgacgag gcggtcggcc tcgcgtcgga gctgtcgccg
     3361 gatgtggtgc tgatggacat ccggatgccg gggaaggacg ggctcgccgc gacgcgggcg
     3421 atcgcggagg acgaacgcct ggccggcgtc aggatcgtcg tactgacgac gttcggcctg
     3481 gacgagtacg tcttcgaggc gatccgatcg ggcgcaagcg gcttcctggt gaaggacacg
     3541 gagccggacg agctcgtgca ggcggtgcgc gtcgtcgccg gcggggacgc cctcctctcg
     3601 ccgagcgtca cgcggcagct cctcgcggag ttcgcgagcc gcgcgaagga gccgatccgg
     3661 gccaccggcc tcgaggagct caccgaccgc gaacgcgaga tcgtcgccct cgtcggagag
     3721 ggactgtcga accaggagat cgccgaccgg ttgttcgtga gtcccgcgac cgcgaagacc
     3781 cacgtcagcc gggcgatggg gaagctcggc gctcgggacc gggcccagct ggtcgtgatc
     3841 gcctacgagt ccggcctcgt ccggccggga tggcttggct gagcgtcgcg gatggcgccg
     3901 aacgcgggcg gccggtaccg tcgggtcgtc ggaggcgcgc gtgaaggtcg gcatcatcgg
     3961 taccgggaag atggggcgcg ggttcgccga ggccctgagc accacgcatg aggtcacggt
     4021 gggctctcgc gaccccgaac gggctcgcac caccgccgag aagaccgggg cgtcgggcgg
     4081 cgccacctac gcggacgccg cgaacgccga cgtcgtgatc ctcacggtcc cctggtccgc
     4141 gatggacgac acgcttgccg agctcggcga tgtatccggc accgtcgtcg tcgacgtctc
     4201 ctacccgtac aacaggcggg agcgagaagc tctcgagggc cgttcgaccg ccgagctgat
     4261 ccaagaccga ctcccggccg ccagggtgtt caagggctgg aaccacgtgc atgccaggca
     4321 cctgaccgcc ccggaggtcg acgggatcgc ctcctcggtc ctgatcgccg gggacgacga
     4381 cgaggggaaa gaggcggtgt tcaccctggc ccgcgacatg ggcttccatc ccgtcgacgc
     4441 cggtccgctc cgagcgacgc gcgacctgga gaagctcgtg ggcgtgatgc tgttcgtgaa
     4501 gctcggcgcg atccgcgtgc tctcgagcgg ccgatcgtga cggcggagct ccagacgcgg
     4561 gtcgacgccg cgctccggcg ggccggtcgc cgacccggcc tcctcgtcgt gagcgcgacc
     4621 gatggccagg acacggcgtt cgcatcgcgg gggccgctcc cggacccgcc gtgcgcaccg
     4681 gaacgagcgg tcttcgagat cggctcgatc acgaaggtgt tcacctcgct ccttctggcg
     4741 atcgcggcgg agcgaggtga gctcggcgtg gacgaccccc tcgtcgagca cctcccgcgc
     4801 cggacccgcg tgccgatgcg cgatggtcgg ccgatcaggc tcgtcgatct cgccacccac
     4861 aggtcggggc ttcctcgcct gccccccggg ttcctcctgg acgcgctccg ccaccgcgac
     4921 gatccctacg cacgcctctc gacggaggac atcctcgcag cgctcgggaa gacccgtccg
     4981 agagtcgccg ccggcgagcg attccactac tcgaactacg gcgcgggagt gctgggcatc
     5041 gcgctcgccc aagcggccgc gaccgactac gaagccctcg tgcgggaacg gatcaccagg
     5101 ccactcggcc taagggacac cgtgatcacg atgagcgagg accatcgggg ccgtctggcc
     5161 gc
//
LOCUS       DASUVE010000269         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_132730, whole genome shotgun sequence.
ACCESSION   DASUVE010000269 DASUVE010000000
VERSION     DASUVE010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132730"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VF036_05855"
     CDS             <1..770
                     /locus_tag="VF036_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6264785.1"
                     /translation="TRDLHRRVPDPPGDDEISRLAGTMNRMLERLEAGQHRARRFVSD
                     ASHELRSPVAAIRQFAEVALAHPERTAVGELAEVVLEEDARLQRLVEDLLLLTRIDEG
                     TIPHPTGAVDLDDLLLEEAARLRATTDLKVDVRSVSAGRVIGDAEQLERLIRNLTDNA
                     SRHARGAVRLSLREVDGRTILCVDDDGIGVDPAEREHIFQRFVRLDDARDRDSGGSGL
                     GLAIVREVATFHGGSVRILDNDLGGTRFEVDLPAQPD"
     gene            822..1514
                     /locus_tag="VF036_05860"
     CDS             822..1514
                     /locus_tag="VF036_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEX6264786.1"
                     /translation="MGQLMSDVTTGSSRQVFPEEITRLTSQQIIRLLFSEPTAEAAQR
                     ATSSFRGTSPIAFAVPSDTELRCRLFLERLRWPHGVRCPRCDDSRRICRIEERAQFEC
                     GSCGYQFSVRAGTILHASHLPLWKWLLAVYLMASADDITSYRLQRTLGISYKTASHLT
                     HTIRALMVDASNGVDWHSGRVRGRVERPIASFPEHAERTRTSGDRGLLVQLLSRLVDA
                     GSGSSDRLVASA"
     gene            complement(1548..>2249)
                     /locus_tag="VF036_05865"
     CDS             complement(1548..>2249)
                     /locus_tag="VF036_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264787.1"
                     /translation="SGSIRDARVLVVMMVLSAACGFDGFENLASNAACDPASRDFSID
                     IDNPFFPLPVGHQVVLEGEEGSTHLLVRITSLDETETVAGVETRVVEEFESKDGTVVE
                     ISRNFFAQATDGTVCYFGEDVDIFDSGGDVTSHSGEWRAGEGENQPGIFMPPSLEVGV
                     AFQQEIAPGIAEDQARVVAIGESTDVPAGTFDDTVTMEDGSPLDGGTGTKVYARGIGL
                     IVDGPARLTRYSPVS"
BASE COUNT          375 a          752 c          731 g          391 t
ORIGIN      
        1 cgacgcgcga cctccatcgg agggttcccg acccaccggg tgatgacgag atctcccgcc
       61 tcgcgggcac gatgaaccgg atgctcgagc ggctggaggc cggccagcac cgggcgcgtc
      121 gtttcgtctc ggacgcttcc catgagctcc gatcgccggt cgccgcgatc cgtcagttcg
      181 cggaagtcgc cctggcccat ccggagcgga cggccgtcgg tgagttggcg gaggtggtgc
      241 tcgaggagga tgcgcgactc cagcggctcg tcgaggacct cctcttgctc acgaggatcg
      301 acgagggaac gatcccccac ccgacgggag ccgtcgatct cgacgatctg ctcctggagg
      361 aggctgcccg gctccgcgcg accaccgacc tgaaggtcga cgtccgctct gtctccgcag
      421 gacgggtcat cggagacgcc gagcagctcg aacggctcat ccggaacctc accgacaacg
      481 cctcccgaca cgctcgaggt gcggtccgtc tctcgcttcg ggaggtcgac gggcgcacga
      541 tcctctgcgt cgacgacgac gggatcggcg tcgatccggc cgagcgagag cacatcttcc
      601 aacggttcgt ccgcctcgat gacgcgcgag atcgagactc cggggggagc gggctcggat
      661 tggcgatcgt ccgagaagtc gcgacgttcc acggcggttc ggttcggatc ctcgacaacg
      721 acctcggagg cactcggttc gaggtcgatc taccggcaca acccgactga atcgcgcacg
      781 ttcagcgaac gttcaggggt ggccggatag cctcgatcga tatgggacag ctcatgtcgg
      841 acgtgacgac cgggagttct cgacaggtgt tccccgagga gatcacccgg ctgacctctc
      901 agcagatcat ccggttgctc ttctcagaac caacggccga agcggcgcag cgggcgacgt
      961 cctccttccg gggcacctcg ccgatcgctt tcgcggttcc gtccgacacc gagctccggt
     1021 gcaggctctt cctcgagcga ctgcgctggc cccacggcgt tcgctgcccg cgctgcgatg
     1081 acagccggag gatctgccgg atcgaggagc gagcccaatt cgagtgcggc agctgcgggt
     1141 accagttcag cgttcgggcc ggtacgatcc tgcatgcctc gcacctcccg ctctggaagt
     1201 ggctcctggc ggtctacctg atggcctcgg cagacgacat cacgtcgtat cggctccaac
     1261 ggacgctcgg gatctcctac aagacggcct cgcacctcac gcatacgatc cgagcgctca
     1321 tggttgatgc ttcgaatggg gtcgactggc attccgggcg ggtccgcggg cgggtcgagc
     1381 gccccatcgc gtcgttcccg gagcatgccg agcgaacgag gacgagcggg gatcggggcc
     1441 tcctggtgca gctgctgtcg agactcgtcg acgctggctc cggctcatcc gaccggctgg
     1501 tagccagcgc gtagctggct cgaagagcgg atgtccttga ggaccgttca ggacacaggc
     1561 gagtagcgcg tcagtcgcgc cggcccatcg acgatcaggc cgatccctcg ggcatagacc
     1621 ttcgtccctg tgccgccgtc caagggactg ccatcctcca tcgtcacggt gtcgtcgaac
     1681 gtcccggcgg ggacgtccgt cgactctccg atcgcgacga ccctcgcctg atcctcggcg
     1741 atccccggtg cgatctcctg ctggaaggca actccaacct cgagcgaggg tggcatgaag
     1801 atcccgggtt gattctcacc ctcccccgcc ctccactcgc cggagtgtga agtgacgtcc
     1861 cctccgctgt cgaagatgtc gacgtcttcg ccgaagtaac agacggtccc atccgttgcc
     1921 tgggcgaaga agttgcggga gatctcgacg accgtcccat ctttggactc gaattcctcc
     1981 accacccgcg tctcgacacc ggcgacggtc tcggtttcgt ccagcgaggt gatccggacg
     2041 aggaggtgtg tcgatccttc ctcgccctcg aggaccactt ggtggccgac gggaagaggg
     2101 aagaagggat tgtcgatgtc gatggagaag tcgcgtgatg cgggatcgca cgcggcgttg
     2161 ctcgcgaggt tctcgaatcc gtcgaatcca cacgcggccg aaaggaccat catcacgacg
     2221 aggactcggg catcacggat ggaaccgct
//
LOCUS       DASUVE010000270         3264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_885973, whole genome shotgun sequence.
ACCESSION   DASUVE010000270 DASUVE010000000
VERSION     DASUVE010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885973"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VF036_05870"
     CDS             complement(<1..304)
                     /locus_tag="VF036_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6264788.1"
                     /translation="MTASETDELLVRRFQNGSSDAFETLVQRHSTRVYNLCLRILGDA
                     EEAADASQDTFLAALRKLSSFRGDAAFTTWLHRVAVNACYDSLRKKRRRPMLQLAPD"
     gene            complement(316..717)
                     /locus_tag="VF036_05875"
     CDS             complement(316..717)
                     /locus_tag="VF036_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264789.1"
                     /translation="MNARRDEAGVVGKMVVVWLLFLVVIAVAAIDAASIAFTTYKLSD
                     VGSTAASEGALVFKADHDVRQTCARVAKVVTAQEPTAKVARGGCSVEAPTGLVTVKIQ
                     KLASTVLAHRISWTEDFAAVTVTETAGPPSL"
     gene            complement(714..2309)
                     /gene="murJ"
                     /locus_tag="VF036_05880"
     CDS             complement(714..2309)
                     /gene="murJ"
                     /locus_tag="VF036_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX6264790.1"
                     /translation="MRNAAVMTVGTTLSRLTGFLRLSAMTAALGVTISTLGSIYTVAN
                     LTPNIVYELILGGILTSVFVPVFAARLETHGTEDAREVADRVISLVAVILVTVAALGA
                     VFAEQIIRLYLVSSDAPDRDAQIELGVFFLRWFMPQIVFYGVGSVVIGLLQAHRRFAA
                     PMFAPILNNLVVIGTFIAYAAARGSRPPDVRTITDLERTILAVGTTLGVVVMTLALWP
                     SLRAIGYRIRLRLTWRHEAVRQLVRLAGWVAVYVAANQLAYVVVIVLNNRFDAGPQVY
                     TTAFIVFQLPHAIFAVSIFTALLPGMSQRWATGDAAGVRSFLSRGLRDTAVVTIPAAI
                     GLVALAEPIARLLFEHGAAVEADAVAIGSALQGFAVGLLFFSSFQLMTRAFYAMQDTR
                     TPAIANVGVGCVNIAAALLYTAAPLELGVQGMALAHATSYVAGAGILLILLRKRLGAL
                     DGARIARTVALTSVAGIASAGVAVGVFRIWDLPSDAGVLSQAAHVLVAIAGGVLVFLI
                     SALILRVGEVDDLKKVIVRRFRG"
     gene            complement(2354..>3264)
                     /locus_tag="VF036_05885"
     CDS             complement(2354..>3264)
                     /locus_tag="VF036_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264791.1"
                     /translation="VSLLLPDPGATSILSDPDVREDPILAAHVLLGELATIWKEQPVP
                     PEDVARGLALDLAPDLPAAFWHPAVRRLTQAPFLESVHAGELSRQVLPPPEPATLGPQ
                     PAEVFLSSYTADLVEAAQRVNTFGSVVEEPAGEADRLRRGLLYAEASQYIRNEGSGRI
                     WINWVNDVIDGTFDAISPDTSRVLTFTSRSGTIPLRMGDPGARVLTVRVELASVRVDL
                     LGAGARPVRITEPNQVITFDAEVRAAGTSEVDVIVRSPDGVELSRAVLVVRSTAINPI
                     ALAITIGAGLVLIALWSRRLFRRRQT"
BASE COUNT          568 a         1066 c         1168 g          462 t
ORIGIN      
        1 cgtcgggggc cagctggagc atcgggcgtc gccgcttctt gcggagggag tcgtagcatg
       61 cgttgacggc gacccggtgc agccatgtcg tgaaggcggc atctcctcgg aaactgctca
      121 gcttgcgcag ggcggcgagg aacgtgtcct gagaggcgtc ggcggcttcc tccgcgtcgc
      181 cgaggatccg cagacacagg ttgtagacgc gcgtgctgtg tcgctgcacg agggtctcga
      241 acgcgtcgga gctcccgttc tggaaccgtc ggacgagcag ttcgtcggtc tcggaggcag
      301 tcaccgcgtc gaacgctaca gcgagggtgg tccggcggtc tcggtgacgg tgacggcagc
      361 gaagtcctcc gtccaggaga tgcggtgagc gaggacggtg ctcgccagct tctggatctt
      421 cacggtcacg aggccggtcg gtgcctccac gctgcagccg ccgcgcgcga ccttcgccgt
      481 gggctcctgc gcggtcacca ccttcgcgac gcgagcacag gtctggcgga catcgtgatc
      541 ggccttgaag acgagcgcgc cttcactcgc cgcggtggat ccgacgtcgg agagcttgta
      601 ggtcgtgaac gcgatcgagg ccgcatcgat cgccgcgacg gcgatcacga cgaggaagag
      661 gagccagacg acgaccatct tgcccacgac ccccgcttcg tcgcgtcgag cgttcatcct
      721 cggaacctcc tgacgatgac cttcttcagg tcatcgacct ctccgactcg caggataagg
      781 gccgagatca ggaacaccag caccccaccc gcgatggcga cgagcacgtg tgcagcctgc
      841 gaaagcaccc ccgcgtccga agggaggtcc cagattcgga acacgccgac ggcgactccg
      901 gcggaggcga tccccgcgac cgaggtcaac gcgacggttc tcgcgatcct cgctccgtcg
      961 agggctccca gcctcttccg gagaaggatc aggaggatcc ctgcgcccgc gacgtacgag
     1021 gtcgcgtgcg ccagggccat cccttggaca ccgagctcga ggggggccgc cgtgtacagg
     1081 agggccgcgg cgatgttcac gcacccgacc cccacgttcg cgatggccgg cgttctcgtg
     1141 tcctgcatcg cgtagaacgc tcgggtcatg agctggaacg acgagaagaa cagcagaccg
     1201 acggcgaagc cctgcagggc gctgccgatg gcgaccgcat ccgcctcgac ggcagctccg
     1261 tgctcgaaga ggagccgggc gatcggctct gcgagcgcga cgaggccgat ggcggccggg
     1321 atcgtgacga cggcggtgtc gcggagtcca cgggacagga acgagcgcac acccgcggcg
     1381 tcgccggtcg cccagcgttg gctcatccca ggcaggagcg ccgtgaagat cgagacggcg
     1441 aagatcgcgt gagggagctg gaacacgatg aaggcggtgg tgtagacctg cgggccggcg
     1501 tcgaagcggt tattgagcac gatcaccacc acgtacgcga gctggttggc ggcgacgtag
     1561 acggcgaccc accccgccag gcgcaccagc tgccggacgg cctcgtggcg ccacgtcagc
     1621 cggagccgga tccggtaccc gatcgcgcgg agggagggcc acaacgccag ggtcatcacg
     1681 acgaccccga gcgtcgtccc cacggcgagg atggtccgct cgagatcggt gatggtccga
     1741 acatctggtg gtcggctccc tcgcgcggcc gcgtaggcga tgaaggtccc gatcacgacc
     1801 aggttgttca ggatcggcgc gaacatcggc gccgcgaacc gccggtgggc ctggaggagc
     1861 ccgatgacga ccgagcccac gccgtagaag acgatctggg gcatgaacca ccggaggaag
     1921 aagacgccga gctcgatctg cgcgtcacgg tcgggagcgt ccgaggagac gaggtacagg
     1981 cggatgatct gttccgcgaa caccgccccc agggcggcga ccgtgaccag gatcaccgcc
     2041 acgagggaga tcacacggtc ggccacttca cgggcgtcct cggtgccgtg cgtctcgagg
     2101 cgcgcggcga agaccgggac gaacaccgag gtgaggatgc ccccgaggat cagctcgtag
     2161 acgatgttgg gcgtgaggtt ggcgacggtg tagatcgagc ccagcgtgct gatggtgacg
     2221 ccgagcgcgg cagtcatcgc gctgaggcgg aggaacccgg tcaggcgcga gagcgtggtc
     2281 cccacggtca tgacggcggc attgcgcacc agggattccc gcgagtcccg gtggggacgt
     2341 ccgtgctccg agctcacgtc tgccgccggc ggaacaatct cctcgaccac agggcgatga
     2401 ggaccaggcc ggctccgatg gtgatcgcca gggcgatcgg attgatcgcg gtggatcgaa
     2461 cgaccaacac cgcccgggac agctccacgc cgtcgggaga tcgcacgatc acgtcgacct
     2521 cgctggtccc ggctgccctc acctccgcat cgaacgtgat cacctggttc ggctcggtga
     2581 tccgcaccgg ccgcgctccg gcgccgagga ggtcgacccg aaccgacgcc aattccacgc
     2641 gcacggtcag gacacgcgca ccggggtcgc ccatgcggag agggatcgtg cccgatcgcg
     2701 atgtgaacgt gaggacgcgc gacgtgtccg gagagatcgc atcgaacgtt ccgtcgatca
     2761 cgtcgttcac ccagttgatc cagatccgac cggacccctc gtttcggatg tattgcgacg
     2821 cctccgcgta cagcaggcct cgccggagcc gatccgcctc gcccgccggc tcctcgacga
     2881 cgctgccgaa cgtgttcacg cgctgggcgg cctcgacgag gtcggccgtg taggaggaga
     2941 ggaatacctc ggccggttgc gggccgaggg tcgccggctc ggggggaggc agcacctggc
     3001 gggacagttc tccggcatgg accgactcca agaacggcgc ctgggtgagt cggcgcaccg
     3061 cggggtgcca gaacgcggcc ggcagatcgg gcgcgaggtc gagggcgagg ccacgcgcca
     3121 cgtcctcggg aggtacgggc tgctccttcc agatcgtggc gagctcgccg aggaggacgt
     3181 gcgccgccag gatcgggtcc tctcggacat cggggtcact caggatcgac gtcgctccgg
     3241 ggtcgggaag gaggagagac acat
//
LOCUS       DASUVE010000271         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1075398, whole genome shotgun sequence.
ACCESSION   DASUVE010000271 DASUVE010000000
VERSION     DASUVE010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075398"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1728
                     /locus_tag="VF036_05890"
     CDS             <1..1728
                     /locus_tag="VF036_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX6264792.1"
                     /translation="LTLNKMTAREMAIPGQNRFTVTGEGYSTQGEIQHVGGSRIDLDP
                     YLLPMILCADAVLEGDGLIGDPTEGALIVLGAKGGLDVDETRATYPRIAEVPFDSEYK
                     FMATFHEMTEPGRGHVVRCYVKGAPDVLISRGGSYRSPDGTLVPITDDNRHLALEAND
                     RMAEGGERVMVVAQRDFDPATFDRAGDLLSLVEDLTLLAMVGIVDPPRPEARDAIARC
                     KSAGIRVRMITGDHATTAGAIGRELGIEGRAITGAEFAAMSDDELLRELPDIGVIARV
                     APEDKVRLVRMLQRMENIVAMTGDGVNDAPALKSANIGVAMGITGTEVSKEAAVMILT
                     DDNFATIVGAVEYGRGLYDNLLKYLRFQMSTLVAYIAIFIGAGIFAIAGGIPLDPLQI
                     LWLNMVIDIPIAIALGFDDPTPGLMERAPRPVGAPVLSTPNWVRLCAQGFVMTVGTLI
                     AYQIGESNYDATVASTMLLTTLSLFHLAAGFLAREQVHTIFDRRLVPGATQLRRYAVS
                     LAAIILVTKIGFLQRIFSMVDLSLEQWWICLGLASSLVVVEELIKVFIRRRSRAGVPA
                     GIDRVPAVA"
     gene            1744..2745
                     /gene="argF"
                     /locus_tag="VF036_05895"
     CDS             1744..2745
                     /gene="argF"
                     /locus_tag="VF036_05895"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011261198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX6264793.1"
                     /translation="MGYNLRNRSYVKELDFTPDELKFLLKLSAELKAAKYGGYEQPRL
                     AGKNIALIFEKTSTRTRTAFEVAAHDQGAQVTYLEPTGSQIGHKESMKDTARVLGRTF
                     DGIEYRGFAQETVEILAEFAGVPVWNGLTNEFHPTQTLADVLTMTEHSDKHLGEIAYC
                     YLGDARNNMGNSLMVAGCKFGMDIRLCAPEHLWPEEELVKTARGIAEETGARLTLTAD
                     VDEGVKGVDYLYTDVWVSMGEDPGVWEERIKLLKPYQVNGEAIKKTGNPRVRFLHCLP
                     AFHNRETKVGEEIFEKYGLEGMEVTDEVFESEHSIVFDQAENRMHTIKAVMVATWGS"
     gene            2749..>2988
                     /locus_tag="VF036_05900"
     CDS             2749..>2988
                     /locus_tag="VF036_05900"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017274577.1"
                     /note="reversible synthesis of carbamate and ATP from
                     carbamoyl phosphate and ADP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX6264794.1"
                     /translation="MRVVVALGGNALLKRGEPMTADVQRANVRTAAPPLAAVAKEHQL
                     VLSHGNGPQVGLLALQAASYEEVEAYPLDVLGAQTE"
BASE COUNT          533 a          964 c         1040 g          451 t
ORIGIN      
        1 ctcacgctca acaagatgac cgcccgcgag atggcgatcc cggggcagaa ccggttcacg
       61 gtcaccggcg agggctattc cacccagggt gagatccagc acgtcggggg atcacggatc
      121 gacctcgacc cctatctgct cccgatgatc ctgtgcgccg acgccgtcct ggagggcgac
      181 gggctgatcg gcgaccccac cgaaggcgcc ctgatcgtgc tcggcgccaa gggcgggctg
      241 gacgtggacg agacccgcgc cacgtacccg cggatcgcgg aggtcccgtt cgactccgag
      301 tacaagttca tggcgacgtt ccacgagatg accgaaccgg gccgggggca cgtcgtccgc
      361 tgctacgtga agggcgcgcc cgacgtcctg atctcgcgcg gtggatcgta ccggagcccc
      421 gacgggacgc tcgtcccgat caccgacgac aaccggcacc tcgccctcga ggcgaacgac
      481 cgcatggcgg agggcggcga gcgcgtgatg gtcgtcgcgc aacgtgactt cgaccccgcc
      541 acgttcgatc gggccggcga cctgctgagc ctcgtggaag atctcacgct cctcgcgatg
      601 gtggggatcg tcgacccgcc caggccggaa gcgagggatg cgatcgcgcg gtgcaagagc
      661 gcagggatcc gtgtacgcat gatcacgggc gaccacgcca cgacggccgg ggcgatcggc
      721 cgtgagctcg ggatcgaggg gcgcgcgatc accggcgccg agttcgcggc gatgagcgac
      781 gacgagctcc tccgggagct gcccgacatc ggggtgatcg cccgggtcgc ccccgaggac
      841 aaggtccggc tcgtccggat gctccagcgg atggagaaca tcgtcgcgat gaccggggac
      901 ggcgtgaacg acgcccccgc gctcaagtcg gcaaacatcg gcgtcgcgat ggggatcacg
      961 ggcaccgagg tctcgaagga agccgcggtg atgatcctca ccgacgacaa cttcgccacg
     1021 atcgtgggtg ccgtcgagta cgggcggggc ctctacgaca acctgttgaa gtacctgcgg
     1081 ttccagatgt cgacgctcgt cgcctacatc gcgatcttca tcggggcggg catcttcgcc
     1141 atcgcgggtg gcatcccgct ggatccgctc cagatccttt ggctcaacat ggtgatcgac
     1201 atcccgatcg cgatcgcgct cggcttcgac gatcccaccc cgggcctgat ggaacgtgct
     1261 ccgcgccctg tgggggcgcc cgtcctctcg acgccgaact gggtgcgact gtgcgcgcaa
     1321 gggttcgtga tgacggtggg cacgttgatc gcgtaccaga tcggcgagtc caactacgac
     1381 gcgacggtgg cctccacgat gctcctcacc accttgtcgc tgttccacct cgcagcaggc
     1441 ttcctcgccc gggagcaggt gcacacgatc ttcgatcgga ggctggtgcc gggagccacg
     1501 cagctccgcc ggtacgccgt gtccctggcg gcgatcatcc tcgtgacgaa gatcggattc
     1561 ctgcaacgca tcttctcgat ggtcgacctg agcctcgagc agtggtggat ctgcctcggg
     1621 ctggcatcga gcctggtggt ggtcgaggag ctgatcaagg tcttcatccg ccgccgctcc
     1681 cgagcgggtg tgcctgcagg catcgaccgg gtgccggccg tggcgtaagg aaggggaacg
     1741 gacatgggat acaaccttcg caaccgcagc tacgtgaagg agctcgattt cacacccgac
     1801 gagctgaagt tcctgctgaa gctctccgcc gagctgaagg cggcgaagta cggcgggtac
     1861 gagcagccga ggctggccgg caagaacatc gccctgatct tcgagaagac gtcgactcgg
     1921 accaggacgg cgttcgaggt ggccgcccac gatcaaggag cccaggtcac ctacctcgaa
     1981 ccgaccggct cccagatcgg gcacaaggaa tcgatgaaag acacggcccg cgtgttgggc
     2041 cgcacgttcg acgggatcga gtaccgcggg ttcgcgcagg agacggtcga gatcctggcg
     2101 gagttcgcgg gcgtccccgt gtggaacggc ctgacgaacg agttccatcc gacgcagacc
     2161 ctggccgacg tgctcacgat gaccgagcac agcgacaagc acctcggcga gatcgcgtac
     2221 tgctacctgg gcgacgcgcg gaacaacatg ggcaactccc tgatggtcgc cgggtgcaag
     2281 ttcgggatgg acatccggct ctgcgcgccg gagcacctct ggccggagga ggagctggtc
     2341 aagacggccc gcgggatcgc ggaggagacc ggcgcccgcc tcacgctcac ggcagacgtc
     2401 gacgaaggcg tgaaaggcgt ggactacctg tacaccgacg tgtgggtctc gatgggcgag
     2461 gacccgggtg tctgggagga gcggatcaag ctcctgaagc cgtatcaggt gaacggcgag
     2521 gcgatcaaga agaccgggaa cccccgtgtc cggttcctcc actgtctgcc ggcgttccac
     2581 aaccgcgaga cgaaggtggg ggaggagatc ttcgagaagt acggactcga ggggatggag
     2641 gtgaccgacg aggtgttcga gtcggagcac tcgatcgtct tcgaccaggc cgagaacagg
     2701 atgcacacga tcaaggccgt gatggtggcc acgtggggga gctgagcttt gcgggtcgtg
     2761 gtggcgctgg gcgggaacgc cctcctgaag cggggggaac cgatgacggc cgacgtccag
     2821 cgggcgaacg tccgcacggc ggccccgccg ctcgccgccg tcgcgaagga gcaccagctc
     2881 gtcctgtccc acggcaatgg tccccaggtc ggactcctcg cgctccaggc ggcctcgtac
     2941 gaggaggtcg aggcctaccc gctcgacgtg ctcggcgcgc agaccgag
//
LOCUS       DASUVE010000272         2686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_512929, whole genome shotgun sequence.
ACCESSION   DASUVE010000272 DASUVE010000000
VERSION     DASUVE010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512929"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VF036_05905"
     CDS             <1..595
                     /locus_tag="VF036_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6264795.1"
                     /translation="FVEREVYDDFVRLLKEKTEKIKVGNPIERDVFLGPVVNEEALNT
                     FEQAAGEAKKNGNVITGGERITEGDLARGLFVQPTVVEAPEDSWLWKKELFVPFVAVA
                     PFDELDDAIDKANDTEYGLTAGFFSKDRHEIQEWLDKIQAGVVYVNRRAGATTGAWPG
                     VQPFGGWKGSGTTGKAGGGPYYVLQYLREQSRTVIEE"
     gene            592..1932
                     /locus_tag="VF036_05910"
     CDS             592..1932
                     /locus_tag="VF036_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017317233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HEX6264796.1"
                     /translation="MRQPEGLAGAEAPRLHTELPGPKAREVLERDARTTSPSLPRAYP
                     FVPARGAGSVVEDVDGNVFLDVNAGIAVTSTGHCHPTVVEAIQRQAADLIHYSASDFY
                     LPIYSELTDRLAEIAPMPDARSFLTNSGTEAVEAGIKLARWSTGRQYVIAFYGSFHGR
                     SYGSVSLTASKATYRAGFGPLLPGVLHAYYGDGRDMPVAPDADAEGFATLDHIEGFLM
                     KRIAAPEEVAAIVVEPVMGEGGYIVPPDGWLQALRELCDRHGILLICDEVQSGIGRTG
                     TMWAVEHFGVEPDILLAGKGIASGMPLGAMIAKDSVMTWTKGTHGSTYGGNPLSCAAA
                     IATLDVIAHEELLENAAKVGDLTMNGLRDIQSRHDAIREVRGLGLMIGIELPDHDAAA
                     AVERGTFERGLLMLGCGDSSIRMSPALGFREDQARVALELFEEVVAETAPGAGA"
     gene            1947..2327
                     /locus_tag="VF036_05915"
     CDS             1947..2327
                     /locus_tag="VF036_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6264797.1"
                     /translation="MLALDSVTFDCADVMRVARFWADALGWELDPESEPDGARVVRDP
                     ARRSDSLYFQPVPEPKVVKNRLHLDLRASGSMAAEVERLEGLGATVVGRVDEEGSFWT
                     VMQDPEGNELCVLRGPEDGWTPDA"
     gene            2371..>2686
                     /locus_tag="VF036_05920"
     CDS             2371..>2686
                     /locus_tag="VF036_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEX6264798.1"
                     /translation="MTKVATMRDAVADLVRDGDTVAIEGFTHLICFAAGHEIIRQRTR
                     DLTLARMTPDVVYDQMVGAGVAKKLIFSWLGNPGVGSLHAVRRRIEDRDPAPLEIEEY
                     SHF"
BASE COUNT          430 a          873 c          995 g          388 t
ORIGIN      
        1 cttcgtggaa cgcgaggtct acgacgactt cgtgcggctg ctgaaggaga agaccgagaa
       61 gatcaaggtc ggcaatccga tcgagcgcga cgtgttcctc gggcctgtgg tcaacgagga
      121 ggcgctgaat acgttcgagc aggcagcggg ggaggcgaag aagaacggga acgtgatcac
      181 cggcggcgag cggatcaccg aaggcgacct cgcgcgcgga ctgttcgtgc agccgacggt
      241 cgtggaggcg cccgaggaca gctggctctg gaagaaggag ctgttcgtgc ccttcgtggc
      301 cgtcgcgccg ttcgacgagc tggacgacgc gatcgacaag gcgaacgaca ccgagtacgg
      361 gctcacggcc gggttcttca gcaaggaccg ccacgagatc caggagtggc tcgacaagat
      421 ccaggcgggt gtcgtctacg tgaaccggcg ggccggggcg accaccggtg cctggccggg
      481 cgtccagccg ttcggcggct ggaaggggtc ggggacgacc ggcaaggccg gcgggggccc
      541 ctactacgtg ctgcagtacc tgcgcgagca gtcccggacg gtgatcgagg agtgaggcag
      601 ccggagggtc tggcgggggc ggaagccccc aggctgcaca cggagctgcc cgggccgaag
      661 gcccgggaag tcctcgaacg ggacgcgcgt acgacgtcgc cgtcgttgcc gcgcgcgtat
      721 ccgttcgtcc ccgcgcgcgg ggccggctcc gtggtggagg acgtcgacgg caacgtgttc
      781 ctggacgtga acgctgggat cgcggtcacg tccaccggcc actgccaccc aacggtggtg
      841 gaggcgatcc agcgccaggc cgccgacctg atccactact cggcgtcgga cttctacttg
      901 cccatctact ccgagctcac cgaccgcctc gcggagatcg ccccgatgcc cgacgcccgg
      961 tcgttcctca ccaactcggg gaccgaggcc gtcgaggccg ggatcaagct cgcccggtgg
     1021 tcgaccggac gccagtacgt gatcgcgttc tatggctcgt tccacggccg ctcctacggg
     1081 agcgtctcgc tcaccgcgtc caaggcgacc tatcgagccg gcttcggacc gctgctgccc
     1141 ggcgtgctcc acgcctacta cggggacggg cgcgacatgc cggtggcgcc cgatgccgac
     1201 gccgagggct tcgcgacgct cgatcacatc gagggcttcc tgatgaagag gatcgcggcg
     1261 ccggaagagg tcgcggcgat cgtcgtcgag ccggtgatgg gcgagggcgg ctacatcgtg
     1321 ccgcccgacg gctggctgca ggcgcttcgc gagctctgcg accggcacgg gatcctgctg
     1381 atctgcgacg aggtccaatc ggggattggc cgcacgggaa cgatgtgggc ggtcgagcac
     1441 ttcggcgttg agccggacat cctcctcgcg ggcaagggga tcgcgagcgg catgcccctc
     1501 ggcgcgatga tcgcgaagga ctcggtcatg acgtggacga agggtacgca tgggtccaca
     1561 tacgggggga acccgctctc ctgcgccgcc gcgatcgcga cactcgacgt gatcgcgcac
     1621 gaggagctgc tggagaacgc cgccaaggtc ggcgacctca cgatgaacgg gctgcgtgac
     1681 atccagagcc gccacgacgc gatccgcgag gtgcgcgggc tcgggctgat gatcgggatc
     1741 gagctgcccg accacgacgc ggcggcggcc gtcgagcgag gcacgttcga gcgcggcctg
     1801 ctgatgctcg ggtgcggaga ctcgtcgatc cggatgtcgc ccgccctcgg gttccgcgag
     1861 gaccaggcgc gcgtggccct cgagctcttc gaagaagtcg tcgccgagac cgcccccggc
     1921 gccggggcct gaggggagcg acggcggtgc tcgcgctcga ctcggtgacg ttcgactgtg
     1981 ccgatgtgat gcgcgtcgcg cgcttctggg ccgacgccct cggatgggag ctcgacccgg
     2041 agtccgagcc cgacggcgcg cgggtcgtcc gagatccagc ccggcgttcc gacagccttt
     2101 acttccagcc ggtcccggag ccgaaggtgg tcaagaaccg tctgcatctc gacctgcgcg
     2161 ccagcgggtc gatggcggcc gaggtcgaac gcctggaagg tctcggcgcc acggtcgtcg
     2221 ggcgggtcga cgaggagggc agcttctgga ccgtgatgca ggacccggag ggcaacgagc
     2281 tctgcgtgct ccgcgggccc gaggacggtt ggacgcccga cgcctgatgg acgccgggac
     2341 cggatcgatg gaacgcggat caacggcggg atgaccaagg tcgccacgat gcgggacgcc
     2401 gtcgcggacc tcgtccggga cggcgacacg gtggcgatcg aggggttcac gcacctgatc
     2461 tgtttcgctg cgggccacga gatcatccgc cagcgcacgc gggatctcac gctggcccgg
     2521 atgacgcccg acgtcgtcta cgaccagatg gtgggcgccg gcgtcgcgaa gaagctgatc
     2581 ttctcgtggc tcggcaaccc cggcgtgggt tcgctccacg cggtacgccg caggatcgag
     2641 gatcgcgatc cggcgccgct cgagatcgag gagtactcgc acttcg
//
LOCUS       DASUVE010000273         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_322892, whole genome shotgun sequence.
ACCESSION   DASUVE010000273 DASUVE010000000
VERSION     DASUVE010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322892"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="VF036_05925"
     CDS             <1..1521
                     /locus_tag="VF036_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX6264799.1"
                     /translation="FEGGEARPLFRGFHDEWMMGLSLIDDGMAAGFATDEDYRVFVAM
                     GSEVPRQIYRNPEPAGVGREWPQGGGGLSPDGALVCIHHSENGDIDRQALRVLDSRSG
                     ESAGDQVDPELRMEAVAWSPAASARRLLFTQERSGIERPGIWDLNTRDRLDLELSDVT
                     GPVIPLGWTLDGSHILAHNDPGEGMNRLLTIDPVGGSVEETVRQEGTIYEAGFRSGGD
                     LWYRGDSSVVPPSVRDATGATVLELPVDPPPPGTRSRWLTWSNPSGDLITGFLTTPSG
                     SGPFPTIASIHGGPEWHHTDLWDPGLQAFVDEGFAVLQVNYRGSTGRGKAFREELKGN
                     IGFPETEDIVAGVDHVVAEGLADPRALFLEGWSWGGYLTTLIAGLHPDRWRAACAGIP
                     VGDYVAAHYESAPTLRAWDVATMGGSPMDLPDLYRERNPMTYVDRVRAPMLLIAGEHD
                     SRCPLGQVMVYAHALRARGHEVDVHLYGGGHHALQVEEKIAHAARTIDFFRRHLAG"
     gene            complement(1528..>2461)
                     /locus_tag="VF036_05930"
     CDS             complement(1528..>2461)
                     /locus_tag="VF036_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6264800.1"
                     /translation="RWWSIVETYGATILYTAPTAIRAFMRWGTEFPERHDLSSIRLLG
                     SVGEPINPEAWVWYWKVIGGERAPVVDTWWQTETGAILITPLPGITDLKPGSATIPFP
                     GIQADIVDEQGDSVPLGGGYLVLTRPWPAISRTIWGDTDRYVETYFSKFGPEVYVAGD
                     GAKRDEDGYLWLLGRIDDVMNVAGHRLSTFEIESALVDDEKVAEAAVVSRPDNLVGEA
                     IVAFVTLKSGHVGDDAMAAELREHVATKIGKIARPHGIIFTADLPKTRSGKIMRRLLR
                     DVAHGRELGDVTTLANAEIVEQIREMSESAPAEG"
BASE COUNT          364 a          862 c          856 g          379 t
ORIGIN      
        1 ttcgagggcg gggaggcgcg gccgctcttc cgcggcttcc acgacgagtg gatgatgggt
       61 ctgtccctga tcgacgacgg gatggcagcc ggcttcgcga ccgacgagga ctaccgggtg
      121 ttcgtcgcga tgggttcgga agtcccccgc cagatctacc ggaacccgga gcccgcaggc
      181 gtgggccgcg aatggccgca aggaggcggc gggctctctc ccgacggcgc cctcgtctgc
      241 atccaccact ccgagaacgg ggacatcgac cggcaggcgc tccgcgtcct ggactcccgc
      301 tccggcgagt ccgcgggcga ccaggtcgac ccggagctgc ggatggaggc ggtggcctgg
      361 tcacccgccg cgagcgcccg gcggctcctc ttcacgcagg agcgctcggg gatcgagcgc
      421 cccggtatct gggacctcaa cacgcgggat cggctcgacc tcgagctgtc cgacgtgacc
      481 ggtccggtga tccccctggg atggacgctc gacggatctc acatcctcgc gcacaacgac
      541 ccgggggagg gcatgaaccg gctcctcacg atcgaccccg tcggcggatc ggtcgaggag
      601 acggtgcgac aggaggggac gatctacgag gccggcttcc gctcgggcgg cgatctctgg
      661 taccggggcg actcgagcgt cgtgcctccg tccgtccggg atgccaccgg agccacggtc
      721 ctcgagctgc cggtcgaccc tccgccgccg ggaacgagga gccgctggct gacctggagc
      781 aacccgtcgg gcgacctgat caccggcttc ctgaccaccc cgtcgggctc cgggccgttc
      841 ccgacgatcg cgtcgatcca cggcgggccc gagtggcacc acacggatct gtgggatccg
      901 gggctccagg cgttcgtcga cgaggggttc gcggtcctgc aggtcaacta ccgagggtcg
      961 accggacggg gcaaggcctt ccgggaggag ctgaaaggga acatcgggtt cccggagacc
     1021 gaggacatcg tggcgggcgt cgatcacgtg gtggccgagg gcctcgccga cccgcgggcg
     1081 ctgttcctcg aaggctggtc gtggggcggg tacctgacga cgctgatcgc ggggctccac
     1141 ccggaccgat ggcgcgcggc gtgcgcgggg atcccggtcg gcgactacgt cgccgcccac
     1201 tacgagagcg ccccgaccct ccgcgcgtgg gacgtggcca ccatgggagg gtccccgatg
     1261 gatctcccgg acctctaccg cgagcggaac ccgatgacgt acgtggaccg ggtccgggcg
     1321 ccgatgctgc tgatcgccgg cgagcacgac agccgctgcc cactcggcca ggtgatggtc
     1381 tacgcccatg ctctgcgcgc tcgagggcac gaggtggacg tgcacctgta cggcggcggg
     1441 caccacgcgc tgcaggtgga agagaagatc gcccatgccg cacggacgat cgacttcttc
     1501 cggcgccacc tcgccgggta gaccggttca tccttcggcg ggggcgctct ccgacatctc
     1561 gcggatctgc tccacgatct ccgcgttcgc cagcgtcgtc acatcgccca gctcgcgccc
     1621 gtgggcgacg tcgcggagca accggcgcat gatcttcccg gaccgggtct tcggaagatc
     1681 tgcggtgaag atgatcccgt gcggtcgcgc gatcttgccg atcttggtgg cgacgtgctc
     1741 gcgcagctcc gccgccatcg catcgtcgcc tacgtggccg ctcttcaagg tgacgaaggc
     1801 gacgatcgcc tcgccgacca ggttgtccgg tctcgacacc accgcggcct ccgcgacctt
     1861 ctcgtcgtcc accagcgccg actcgatctc gaacgtcgag agccggtgtc cggcgacgtt
     1921 catgacgtcg tcgatcctgc cgaggagcca gaggtacccg tcctcgtccc gcttggcgcc
     1981 gtcacccgcg acgtagacct cggggccgaa cttcgagaag tacgtctcga cgtaccggtc
     2041 ggtgtcaccc cagatcgtgc gcgagatcgc gggccacggt cgggtgagga cgaggtagcc
     2101 gccgccgagc gggacgctgt cgccctgttc atcgacgatg tccgcctgga tccccgggaa
     2161 cgggatggtg gccgagcccg gcttgaggtc ggtgatgccc ggaagcggtg tgatgaggat
     2221 cgctccggtc tcggtctgcc accacgtgtc cacgaccggc gcgcgttcgc ccccgatcac
     2281 cttccagtac cagacccagg cctcggggtt gatcggctca cccaccgacc cgagcagacg
     2341 gatcgaggag aggtcgtgcc tctccgggaa ctccgtcccc catcgcatga acgcccggat
     2401 cgcggtcggt gccgtgtaca ggatggtggc cccgtacgtc tccacgatcg accaccatcg
     2461 a
//
LOCUS       DASUVE010000274         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1453087, whole genome shotgun sequence.
ACCESSION   DASUVE010000274 DASUVE010000000
VERSION     DASUVE010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453087"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VF036_05935"
     CDS             complement(<1..618)
                     /locus_tag="VF036_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX6264801.1"
                     /translation="MTVPRIAVVGRQNVGKSTLVNRLFGKRETIAHDDPGVTRDRVEL
                     EATWNGKRFGLVDTAGYLHRATGVDALAGLQSLRAIDEAQLVLLVVDVATGITEEDAQ
                     LAHRLRRAASPVLAVVNKVDAAEDEAEVPAFLALGLGDPIAVSSKYGRGVGELLDRIV
                     DLLPDAPRAGGDDGEDQEEPRFAIVGRPNVGKSSLFNSLVGEERSV"
     gene            complement(615..1304)
                     /gene="cmk"
                     /locus_tag="VF036_05940"
     CDS             complement(615..1304)
                     /gene="cmk"
                     /locus_tag="VF036_05940"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX6264802.1"
                     /translation="MTVRVVAIDGAAGSGKSTLARLLARELRLPYVNTGSMYRALTLA
                     ALRGSADVEDGAALARLMDTLEFTLSSGTDGELLIDGAPPSDDLETADVEASVSTVAK
                     HPPVRSAMRAAQRALGVGGAVMEGRDIGTVVFPDAPLKLFLVAAPGERAGRRVDERGA
                     TDVAEALHARDRLDARVHPHVPADDAVVIDTSDLGVQGTLRAALDAVASRAPGLLVPE
                     GPRADEEGSRS"
     gene            complement(1301..>2033)
                     /locus_tag="VF036_05945"
     CDS             complement(1301..>2033)
                     /locus_tag="VF036_05945"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX6264803.1"
                     /translation="VPTRDHTERALGALGAPVRATEGGIVLDGPFRHEGFRGKVPGDP
                     SSAAFLLGAAALTGGEVALTGVGLNPSRLVFLEVFERMGVEVDVEVEGSVLGEPVGTM
                     HLAPRGSVRPVHVDAGEVPLVIDEVPILAALAAHADGPSRFGGAAELRVKESDRLSVL
                     ADGIRSLGGLAEEDENDLVIEGGGLRGGSARSAGDHRIVMALTVAAMAAEASSEIDGA
                     EAAAVSFPGFFGLLRSLGADLEEAR"
BASE COUNT          286 a          796 c          685 g          266 t
ORIGIN      
        1 caccgaccgc tcctccccca ccagactgtt gaacaggctg gacttgccca cgttcggccg
       61 cccgacgatg gcgaaacgcg gctcctcctg gtcctcaccg tcgtcacccc cggccctcgg
      121 ggcgtccggg agcaggtcga cgatgcggtc gaggagctcg cccactcccc gaccgtactt
      181 cgaggacacg gcgatcggat cgcccagccc cagcgcgagg aacgcgggca cctccgcctc
      241 gtcctcggcc gcatccacct tgttcacgac cgccaggacc ggggacgccg cccgtcggag
      301 acggtgcgcg agctgggcgt cctcctccgt gatccccgtc gcgacgtcga ccaccaggag
      361 gacgagctgg gcctcgtcga tcgcccgcag cgactgcagg ccggcgagcg cgtcgacgcc
      421 ggtggctcgg tggaggtacc cggccgtgtc gacgagcccg aaccgcttcc cgttccacgt
      481 cgcctcgagc tccacgcggt cccgcgtgac ccccgggtcg tcgtgtgcga tcgtctcgcg
      541 cttgccgaag agcctgttga ccaacgtcga tttcccgacg ttctggcggc cgacgacggc
      601 gatccgtgga acggtcacga ccgtgatccc tcctcgtcgg cccgaggtcc ctcggggacc
      661 aggaggcctg gcgcccggct ggccaccgcg tcgagcgcgg cacggagcgt cccctggacg
      721 ccgagatcgc tcgtatcgat caccacggcg tcgtccgccg ggacgtgggg atggaccctc
      781 gcatcgaggc ggtcccgcgc gtggagtgcc tcggcgacat cggtcgcgcc ccgttcgtcg
      841 acccggcggc ccgcacgctc gccgggcgcg gccacgagga agagcttcag cggggcatcg
      901 gggaacacga ccgtgccgat gtcgcgcccc tccatcacgg ctccgcccac acccagagca
      961 cgctgggcgg cgcgcatcgc cgagcgcacc ggagggtgct tcgcgacggt gctgaccgag
     1021 gcctcgacgt ccgccgtctc gaggtcgtcg gagggcggcg cgccgtcgat caggagctcc
     1081 ccgtccgttc cggagctcaa cgtgaactcg agggtgtcca tcagccgggc gagcgccgcc
     1141 ccgtcctcga catccgcgga tcctcgaagg gccgcgaggg tgagcgcgcg gtacatcgac
     1201 cccgtgttga cgtacggcag ccggagctcc ctcgccagga gccgggcgag cgtgcttttc
     1261 ccgctcccgg cggcgccgtc gatcgccacg acccggaccg tcatcgcgcc tcctcgaggt
     1321 cggcacccag cgagcgcagc agcccgaaga acccagggaa ggagacggcc gccgcctcgg
     1381 caccgtcgat ctcagaggac gcctccgctg ccatcgccgc cacggtgagc gccatcacga
     1441 tccggtgatc gccggcggag cgagcggagc cgccccggag gccgccgccc tcgatcacga
     1501 gatcgttttc gtcctcctcg gccagccccc ccaggctccg gatcccgtcg gcgagcacgg
     1561 agagccggtc gctctccttc acgcggagct cggccgcgcc accgaagcgt gagggaccgt
     1621 ccgcgtgggc ggcgagggcg gcgaggatcg ggacctcgtc gatcaccagt gggacctctc
     1681 cggcgtcgac gtgaaccggc cggaccgatc ccctcggggc caggtgcatc gtccccaccg
     1741 gctcccccag gacggacccc tcgacctcga cgtcgacctc cacacccatc cgttcgaaca
     1801 cctcgaggaa cacgagccgg ctcgggttga gcccgacgcc cgtgagcgcg acctccccgc
     1861 cggtgagcgc ggctgcaccg agcaggaacg cggccgacga cgggtccccc ggcaccttcc
     1921 cgcggaatcc ctcgtgacgg aacggcccgt cgagcacgat ccccccctcc gtcgcgcgca
     1981 cgggggcgcc aagggcgccg agggcgcgct ccgtgtggtc cctcgtggga aca
//
LOCUS       DASUVE010000275         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1265585, whole genome shotgun sequence.
ACCESSION   DASUVE010000275 DASUVE010000000
VERSION     DASUVE010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265585"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VF036_05950"
     CDS             complement(<1..875)
                     /locus_tag="VF036_05950"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6264804.1"
                     /translation="MDDRALRERIAADMPRAVDELARLVRIPSMGHPGYDPANVRASA
                     ETTRDILMGAGVTDASLLELEGGHPAVFGEVRGPDDAPTVLLYAHHDVQPEGPLEEWD
                     TPPFEPVVKDGRMYGRGAADDKSGIVVHASAIRALLADGLPPVSIKVVVEGEEECSTA
                     HLPALVQGNADLLRADVAVIADGGNYRTGVPTLNSSIRGVTDIVVQVRVLPRAQHSGS
                     YGGPIPDAITALSRMIASLHHDDGSVAIEGLQRFGWTGMQIPEDEFREESGILDQVEL
                     IGAGTLSDRLLTGPAV"
     gene            complement(895..1512)
                     /gene="pdxH"
                     /locus_tag="VF036_05955"
     CDS             complement(895..1512)
                     /gene="pdxH"
                     /locus_tag="VF036_05955"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX6264805.1"
                     /translation="MGDAEDEPLDLESLPSEPDVLFRRWYEEAERAGIRLPNAIALAT
                     ASADGRPSIRHVLLRGVEDDAFVFYTNHGSRKGVELAENPRAAFSVYWRELDRQISVT
                     GDVAAVGDEESDAYFATRPREARIGAWASRQSSELASRDELMERVATFDARYQGGDVP
                     RPPFWGGYRVAPITVEFWQGRPHRLHDRFRYARDGDGWTIRRLSP"
     gene            complement(1591..>2086)
                     /locus_tag="VF036_05960"
     CDS             complement(1591..>2086)
                     /locus_tag="VF036_05960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226402.1"
                     /note="involved in translesion DNA polymerization with
                     beta clamp of polymerase III; belongs to Y family of
                     polymerases; does not contain proofreading function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX6264806.1"
                     /translation="VQVGRRRRSMGTQRALGRGSRSPDSLDTDLVALVDRLTRRLRAA
                     HRVCRTVVLRLRFDDLSRATRSHTLWEATAGTQTILAVGRVLLAAAMPMIRDRGITLI
                     GLTLSNLDDDSAVQLSLPFARSLTAGIDTTLDEVRDRFGSSSITRAVLLGRRQGLTVP
                     LLPD"
BASE COUNT          309 a          729 c          751 g          297 t
ORIGIN      
        1 accgccgggc ccgtcagcaa tcggtccgaa agggtgccgg ccccgatcag ctcgacctga
       61 tcgaggatcc cggactcctc gcggaattcg tcctcgggga tctgcatccc cgtccatccg
      121 aaacgctgca agccctcgat ggcgacgctc ccgtcgtcgt ggtgcagaga ggcgatcatg
      181 cgcgagagcg ccgtgatcgc gtcgggtatc ggccccccgt aggagccgga gtgctgcgcc
      241 cggggcagga cccggacctg caccacgatg tcggtcacgc cccggatcga cgagttcaac
      301 gtgggcaccc cggtgcggta gttcccgccg tcggcgatca cggccacgtc ggcgcggagc
      361 aggtcggcgt tgccctggac gagcgcgggc agatgcgcgg tcgagcactc ctcctcaccc
      421 tccacgacga ccttgatcga gaccggtggc agaccgtcgg cgaggagcgc tcggatcgcg
      481 gacgcgtgga cgacgatgcc gctcttgtca tccgcggcgc cgcgtccgta catccgcccg
      541 tccttcacga ccggctcgaa gggcggcgtg tcccattcct cgagcggccc ctccggctgg
      601 acgtcgtggt gcgcatagag caggacggtc ggtgcgtcgt ccgggccgcg gacctcgccg
      661 aagaccgcgg gatggccccc ctcgagctcg agcaggctcg catccgtcac gccggctccc
      721 atcaggatgt cccgcgtcgt ctccgcggag gcgcgcacgt tggcagggtc gtagcccggg
      781 tggcccatcg aggggatccg cacgagacgc gcgagctcat cgaccgcgcg cggcatgtcg
      841 gcggcgatgc gctcgcggag ggcacggtcg tccatggggg tgcgagccta tcggctaggg
      901 cgagagtcgc cggatcgtcc aaccgtcgcc gtcgcgcgcg tatcggaagc gatcgtggag
      961 gcggtgcggt cgtccctgcc agaactccac ggtgatcggc gcgacccggt acccgcccca
     1021 gaacggaggt cgcggaacgt cgccgccctg gtagcgggcg tcgaacgtcg cgacgcgctc
     1081 catcagctcg tcgcgagagg cgagctccga gctctgccgc gatgcccacg cgccgatccg
     1141 cgcctcacga ggccgcgtgg cgaagtaggc gtcggactcc tcgtcgccga ctgccgcgac
     1201 gtcgccggtc acggagatct gtcggtcgag ctctcgccag tagacggaga acgccgcccg
     1261 cgggttctcc gcgagctcga cgcccttgcg gctcccgtgg ttcgtgtaga agacgaaggc
     1321 gtcgtcctcg acaccccgga ggaggacatg gcggatcgac gggcgcccgt cggccgacgc
     1381 cgtggcgagc gcgatcgcgt tggggagacg gatgcccgct cgctccgcct cctcgtacca
     1441 acgccggaac agcacgtccg gctccgaggg aaggctctcg aggtccagtg gctcgtcttc
     1501 ggcgtcgccc acggcgccac cgtatcgcga ctccccgggg ataccggctt cgtgtcggac
     1561 gggcgggacc gcgggctccg gcgaaggtgc tcaatccgga agcagcggca ccgtcagccc
     1621 ctggcgacgc ccgaggagca ccgcgcgggt gatggacgac gacccgaatc gatcgcgcac
     1681 ctcgtcgagc gtggtgtcga tgccggcggt gagcgaacga gcgaacggca aggaaagctg
     1741 gaccgcggaa tcgtcgtcga gattgctgag ggtcaggccg atgagcgtga tccctcgatc
     1801 tcggatcatc ggcatcgccg cggcgagcag gacccttccg acagcgagga tcgtctgggt
     1861 gcccgcggtc gcctcccaga gggtgtgcga cctcgtcgct cgcgagaggt cgtcgaaccg
     1921 caaccgaagg acgaccgtgc ggcacacgcg gtgggccgct cgcagcctgc gcgtgagtcg
     1981 gtcgaccagc gcgacgaggt cggtgtcgag cgagtcgggc gatcgcgatc ctcggccgag
     2041 cgcccgctgt gtgcccatcg accggcggcg gcggccgacc tggacc
//
LOCUS       DASUVE010000276         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1076342, whole genome shotgun sequence.
ACCESSION   DASUVE010000276 DASUVE010000000
VERSION     DASUVE010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076342"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VF036_05965"
     CDS             complement(<1..815)
                     /locus_tag="VF036_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX6264807.1"
                     /translation="MDLVAGRVYADERRPIRHIDGLLLLVTAILIVLGFFLLYSATNQ
                     TLRQDRLDPFLRVNKQILTAAFGVILILAMATFDYRFLKVYAGFVYAGVVVALLLVRI
                     PFLGSSQAGAQRWFEIGGFQVTPSEFAKVGLIIMLAASLSELKHGFPSAQDMIRMCGV
                     ALVPLMLVFIQPDIGTSIVMVAIAAGMFLVAGVRGRHLAALVVVCLVLIAAAFQLGVV
                     KDYQLDRLRAFLDRENTPADVRYNLDQAEIAVGSGGLLGQGYLHGRQTTLDYVP"
     gene            complement(815..>2497)
                     /locus_tag="VF036_05970"
     CDS             complement(815..>2497)
                     /locus_tag="VF036_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX6264808.1"
                     /translation="RLVHNRQSLEVRINRDELGDEAEATLAHLSEILGVSAQELGEEL
                     DSKLYYSYQPVPVAEFVPEEVYFKVREEPERFQGVEVVQQSVRTYPQGPLGAHLVGWV
                     GQINAEEIRQRRFEGYGPSDLVGKAGLEATYERWLRGTPGEERFLVNSDGEVLREFDP
                     KPAQPGHDLRLSLDLDIQRIAEEELLTGIENARTVFDESSGKNLIANAGAVVVLDPKT
                     GGIVAMASWPSFRPSWFVRGLTHGQRSLLFESEQAPMLNRATQITYAPGSTFKPFVAL
                     AAVKERIASFGSYYDCPAEYVHPGDESGTVFENWSGQSGGTLSIAGGLKVSCDSQYYA
                     WGSRFYERDLDTGQEELPRRMRQWGFGRATEVDLPAEAVGTVPDHEYVAEHDEVYPDG
                     WIPGIDILLAIGSGEMKATPLQVAQAYAALASGRLCRPHLVARIEAGDGEVAKRIGGR
                     CRPVDYSAGELDYIREALRGVTTTGGTAGSVFEGCPLDVSGKTGTAERPPFQDTSWFA
                     GMVPAEDPEYVVVATVEQGGFGAETAAPIVRNIMSRIYRTPCEGPDLGEAED"
BASE COUNT          401 a          891 c          818 g          387 t
ORIGIN      
        1 gggacgtagt ccagcgtcgt ctggcgtccg tggaggtatc cctgccccag gaggccgccg
       61 ctcccaacgg cgatctcggc ttggtccagg ttgtagcgga cgtcggcggg tgtgttctcc
      121 cggtcgagga atgcgcgcag acggtcgagc tggtagtcct tcacgacccc gagctggaag
      181 gcggcggcga tgaggacgag gcagaccacg acgagcgccg ccaggtgccg gccccggacc
      241 ccggctacca agaacatccc ggccgcgatc gccaccatca cgatcgacgt tccgatgtcg
      301 ggctggatga agacgagcat caacgggacc aacgccacgc cgcacatccg gatcatgtcc
      361 tgcgcgctcg ggaagccgtg cttcagctcg gacagactcg cggcgagcat gatgatcaag
      421 ccgaccttgg cgaactccga cggcgtcacc tggaagccgc cgatctcgaa ccagcgctgt
      481 gcgcccgcct gcgacgatcc gaggaagggg atgcgaacca gcaggagagc aacgaccact
      541 ccggcataga cgaacccggc gtagaccttg aggaagcggt agtcgaacgt ggccatcgcg
      601 aggatcagga tgaccccgaa ggccgcggtc aggatctgct tgttgacgcg caggaacgga
      661 tccagccgat cctggcggag ggtttggttg gtcgccgagt agagcaggaa gaagccgagg
      721 acgatcagga tcgccgtcac gagcaggagc agcccgtcga tgtgacggat cggccggcgc
      781 tcgtccgcgt agacgcgtcc cgcgacgagg tccatcagtc ctcggcctcc ccgaggtcgg
      841 gtccctcgca cggcgttcgg tagatcctgc tcatgatgtt ccggacgatc ggggcggcgg
      901 tctccgcccc gaaccctccc tgttccacgg tggccacgac cacgtactcc ggatcctccg
      961 caggcaccat gccggcgaac cacgacgtgt cctggaacgg cgggcgctct gcggtcccgg
     1021 tcttcccgga gacgtcgagc ggacacccct cgaacaccga tcccgccgtc cctccggtcg
     1081 tcgtgacacc tcggagggcc tcgcggatgt agtcgagttc gcccgcggag tagtcgaccg
     1141 gccggcacct gccgccgatc ctcttcgcga cctcgccgtc ccccgcctcg atcctggcca
     1201 cgaggtgggg ccggcacagc cgaccgctcg cgagcgccgc gtacgcctgc gcgacctgca
     1261 acggagtcgc cttcatctcg cccgagccga tcgcgagcag gatgtcgatc cccgggatcc
     1321 agccgtccgg atacacctcg tcgtgctcgg cgacgtactc gtggtccgga acggtcccga
     1381 ccgcctccgc cgggaggtcg acctctgtcg cgcgaccgaa gccccactgc cgcatccggc
     1441 gcgggagctc ctcctggccg gtgtcgagat ccctctcgta gaaccggctc ccccaggcgt
     1501 agtactgcga gtcgcacgag accttgagcc cgccagcgat cgacagcgtg ccgccgctct
     1561 gcccgctcca gttctcgaag accgtgccgg actcgtcgcc cgggtgcacg tactcggccg
     1621 ggcagtcgta gtagctgccg aacgaggcga tccgctcctt caccgcggca agtgcgacga
     1681 acggcttgaa cgtcgagccc ggcgcgtacg tgatctgggt ggcgcggttc aacatcggcg
     1741 cctgctcgct ctcgaacaac agggaacgct ggccgtgcgt gagcccccgg acgaaccacg
     1801 acggccggaa cgacggccac gacgccatcg ctacgatgcc acctgtcttg ggatcgagca
     1861 cgacgacggc gccggcgttc gcgatgaggt tcttccccga gctctcgtcg aagaccgtcc
     1921 gtgcgttctc gatgcccgtg aggagctcct cctcggcgat cctctggatg tcgagatcga
     1981 gcgacagccg caggtcgtga ccgggttggg cgggcttcgg atcgaactcc cgcaggacct
     2041 cgccgtcgga gttcacgagg aaccgctcct cccccggcgt cccgcgcagc cagcgttcgt
     2101 acgtcgcctc gagcccggcc ttccccacga ggtccgacgg cccgtacccc tcgaaccgcc
     2161 gctggcggat ctcttccgcg ttgatctgcc cgacccaacc gacgaggtgg gcgccgagcg
     2221 gcccctgggg gtacgtgcgg acactctgct ggacgacctc gacgccttgg aaccgctccg
     2281 gctcctcgcg caccttgaag tagacctcct cgggcacgaa ctccgcgacc gggacgggct
     2341 ggtagctgta gtacagcttc gagtcgagct cctcgccgag ctcctgggcg gagacgccca
     2401 ggatctccga gaggtgcgcg agcgtcgcct ccgcctcgtc ccccagctcg tcgcggttga
     2461 tgcggacctc gaggctctgc cggttgtgga cgagccg
//
LOCUS       DASUVE010000277         6627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_701241, whole genome shotgun sequence.
ACCESSION   DASUVE010000277 DASUVE010000000
VERSION     DASUVE010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701241"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VF036_05975"
     CDS             <1..1013
                     /locus_tag="VF036_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6264809.1"
                     /translation="TITPQKPEPWMPAGRAAPTGAPAPTYAEPIATRKAFGETLRWLA
                     GERDDLVVLDGEVGNSTYTEDAEAVAPDRFFQMYIAEQTLIGTQTGLYALGITAFSAT
                     FGAFFTRAYDQIRMASIGRADLRLCGSHAGVSIGEDGPSQMALEDLAAFRAIHGTTVL
                     YPSDGNATVGLVTSMADLRGISYLRTTREATPVLYGPEEDFPIGGSKTLREGDDVTLV
                     GAGITVHESLEAADRLAADGIEARVLDCYSVKPIDEESLRNAAAATGLLVVVEDHRVE
                     GGLGDAVLDALATGGTLVGRVIKLAVTDMPGSGAPEELREWAGIDAGAIVGAVRSALG
                     RG"
     gene            complement(1020..3275)
                     /locus_tag="VF036_05980"
     CDS             complement(1020..3275)
                     /locus_tag="VF036_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6264810.1"
                     /translation="MDVSQGSSSAAVQAVDPGVSSAVPPLAQVSDALANVGGELGADR
                     VALFENIRDPDGRLWMSLASEWRRNGVRGVFDDPSTTLHPYSPDFIRWIEVLGDHRVL
                     SASVTDLPESERRILAAEGTVSFVAVPIAVDEGWWGFLSVDDCSGGRSWGTEEIELLR
                     VLAGSIAADVHRQRQASADEIVGDRYRSIVEHIPAVTYIDALDDAASTIYMSPQVEQM
                     LGYAPEEWICDPDLWLRIVHPDDRPRAIAENQRHNETGESFSLDYRVYRKDGRVVWIH
                     DEARIVRDERGIPTFSLGVMFDITEMKRGDEKVAFLTYHDELTGLPTRAMFEELLELS
                     IARAKHHEDSVAVICVDIDDFRLVNDSLGRFAGDEVLNVVAERLRQATRETDLVARRS
                     GDTFLLLLADLERDGDDVEAAVARAESAAQRILDSLTVPFRAGGTELYVSASIGVSLF
                     PQDGDEPGSLLRNADAAMLESKKGGHAYVVSNRGALDSSAKLRFVTRLRKAVESQRWA
                     LHYQPVLDLATGRMRGVEALIRWIEADGTMVPPGDFIPVAEELGLIEAIGDWVVRELV
                     YQAQAWRELEIDLSISFNLSPRQFWQPDLASGILDRIREGGIDPAKVTVEITESSAMT
                     DPDRAQTILEALHDGGLRIAIDDFGTGYSSLARIRDMPVDVLKIDRSFVSGVDVDPQK
                     QSIVTAFIELARGLGVTTLAEGIETEGELAFVRERGCVLGQGFYFSKAVPPEEIIAMS
                     FGGLRVASQAS"
     gene            3353..3634
                     /locus_tag="VF036_05985"
     CDS             3353..3634
                     /locus_tag="VF036_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264811.1"
                     /translation="MADYKQVLHERLYVRVPGTDKLRKTAAARLKHLVATGWRETERD
                     RKPDHIWVRFERTGHAPMRARLPKKAPEIQIERRPRRDNRGGGFRGGRR"
     gene            3760..5193
                     /locus_tag="VF036_05990"
     CDS             3760..5193
                     /locus_tag="VF036_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HEX6264812.1"
                     /translation="MSVEFLSPPSDRPGHRDPHAHAAAQPFTYPLQRPYGEPDWTRLA
                     AYRDVTTEQWESAQWQRAHTVKNLKEFKAALGEHLDDELLADIERDQQERATMSMLLP
                     PQMINTMDETDLRADPVRLYMAPAFSDRDREFPAHPFASRDSLHEADMWAVEGLTHRY
                     PTKVLAELIPTCPQYCGHCTRMDLVGNDTVQVLQYKFEAKQNDRWDAMLDYLRRTPSV
                     RDVVVSGGDMANVPIKRLETFVMGLLDIENVRDIRLATKGLMGIPQHFLQDEVRAAAE
                     RMASRANERGVDIAVHTHVNAAQQVTPLVGRATKVFLDAGFRDVRNQGVLLRGVNTAP
                     QQLLELCFMLLDHAKILPYYFYICDMIPNAEHWRTAVWEVQDLQHAIMGYLPGFATPR
                     MVVDVPFVGKRWAHQVAEYDRVRGISGWTKNYRTGIESDDPEAFTRRYHYYDPISTLP
                     EEGKAWWREQLARGVDELIAELQPQHT"
     gene            5222..6076
                     /locus_tag="VF036_05995"
     CDS             5222..6076
                     /locus_tag="VF036_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX6264813.1"
                     /translation="MSRVLTVLSAQVAPVAWDPAATLDKFEDHVRVARRAFPDVDLMV
                     FPELYLTAIDGFTSGGSADWERRVAEEIPGPLTDRVAKIAARAKRWIVAGSINERRGR
                     KIHNTAIAFSPDGEIAAVYRKLFPWRPFEDADPGAEPAPVFTIPGKGVVGMMICYDGW
                     FPEVARGLALQGAELIAQPTATTTPDREEEVVLARANAIVNQVFVFNPNMVSTFGPGR
                     SVSVDPEGRVLFEAGAGEELLVEVVDMDRVAEIRRRGTRGVTRPWRHFLDGPREVVLR
                     PYRARRTT"
     gene            complement(6052..>6627)
                     /locus_tag="VF036_06000"
     CDS             complement(6052..>6627)
                     /locus_tag="VF036_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264814.1"
                     /translation="APDLVDARGEIVELGTSARWTLTFAEPLTVPDAFGRPFRVDIAI
                     RDPDVPPLSFAYYRGVNRLVRVDATVAHETEIYLLPERGANVFDPPLIEGATMTIQIP
                     GRTLSADEDLTGTSPGLEALRWTVIVRDGRACDLLGNGRPTERFGVGIDPVGEDEPAA
                     DARSGLSIWWIVAGMAVATLGIAAYVVRRAR"
BASE COUNT         1076 a         2325 c         2254 g          972 t
ORIGIN      
        1 tcacgatcac gccgcagaag cccgaaccgt ggatgccggc gggccgggcc gcgccgacgg
       61 gcgcgcccgc acccacctat gcggagccga tcgccacccg caaggccttc ggagagaccc
      121 tgcggtggct cgccggcgag cgcgacgacc tcgtcgtcct ggacggcgag gtcgggaact
      181 ccacctacac ggaggatgca gaggccgtcg ccccggaccg cttcttccag atgtacatcg
      241 ccgagcagac cttgatcggg acgcagacgg gcctgtacgc gctcggcatc accgccttct
      301 ccgcgacgtt cggcgcgttc ttcacgcgcg cctacgacca gatccggatg gcgtcgatcg
      361 ggcgggcgga tctccgcctg tgcggatcgc acgcgggggt gtcgatcggc gaggacggcc
      421 cctcccagat ggcgctcgag gacctcgccg ccttccgcgc gatccacggg acgaccgtcc
      481 tctacccctc cgacgggaac gccacggtgg ggctcgtgac gtcgatggcc gacctccgcg
      541 gcatctccta cctccgcacg acgcgagagg ccacgccggt cctgtacgga cccgaggagg
      601 acttcccgat cggcgggtcg aagaccctcc gcgagggcga cgacgtgacg ctcgtcggtg
      661 cgggcatcac ggtccacgag tcgcttgagg ccgccgatcg gctcgcggcc gacgggatcg
      721 aggcgcgtgt gctcgattgc tacagcgtga agccgatcga cgaggagtcg ctgcggaacg
      781 ccgcggccgc gaccgggctg ctggtcgtcg tcgaggacca cagggtcgag ggcggcctgg
      841 gcgacgcggt gctcgacgcg ctggccacgg gagggacgct cgtcgggcgg gtgatcaagc
      901 tcgccgtgac ggacatgccc ggctccggcg cacccgagga gctgcgggag tgggcgggga
      961 tcgacgccgg cgcgatcgtg ggtgcggtgc ggagcgcgct cggccgaggc tgaggtgcgt
     1021 cagctggctt ggctcgccac gcggagaccg ccgaaggaca tggcgatgat ctcctccggc
     1081 ggcacggcct tcgagaagta gaagccctgt cccagcacgc agccgcgctc gcgaacgaac
     1141 gcgagctctc cttccgtctc gatcccctcg gccagcgtgg tgacgccgag ccctcgcgcc
     1201 agttcgatga aggcggtgac gatgctctgc ttctgcggat cgacgtcgac accgctgacg
     1261 aacgaacgat cgatcttgag gacgtcgacc ggcatgtccc ggatccgagc gagcgaggag
     1321 tacccggtcc cgaaatcgtc gatcgcgatc cggaggccgc catcgtgcag cgcctcgagg
     1381 atcgtctgcg cgcggtccgg atcggtcatc gccgaggact ccgtgatctc gacggtgacc
     1441 ttcgcgggat cgatgccacc ttcgcggatc cgatcgagga tcccgctcgc gaggtctggc
     1501 tgccagaact ggcggggaga caggttgaag ctgatcgaca ggtcgatctc cagctcgcgc
     1561 caggcctgcg cctgatagac gagctcgcgc acgacccaat cgccgatcgc ttcgatcaat
     1621 ccgagctcct ccgccaccgg gatgaagtcg cccggcggca ccatcgtgcc gtcggcctcg
     1681 atccagcgga tcagcgcctc caccccgcgc atgcggccgg tcgcgaggtc gaggacgggc
     1741 tggtagtgga gcgcccatcg ctggctctcc acagccttgc gcagcctcgt cacgaaacgg
     1801 agcttggcgg aggaatccag ggcgccgcga ttggacacga cgtacgcgtg cccgcccttc
     1861 ttggactcga gcatcgccgc gtccgcgttc cgaaggaggc tgcccggctc gtcgccgtcc
     1921 tgggggaaca ggctgacccc gatcgaggcg gagacgtaga gctcggtgcc gcccgcgcgg
     1981 aagggcacgg tcagagaatc gaggatccgc tgcgccgccg actccgctcg ggcgacggcc
     2041 gcttcgacgt cgtccccgtc gcgctcgagg tcggcgagca ggagcaggaa cgtgtcgccg
     2101 gatcggcggg cgaccaggtc ggtctccctg gtcgcctggc ggagccgttc ggccacgacg
     2161 ttcaggacct cgtcgccggc gaaccggccg agggagtcgt tcacgagacg gaaatcgtcg
     2221 atgtccacgc agatcaccgc cacggagtcc tcgtggtgct tcgcacgcgc gatcgagagc
     2281 tcgagcagct cctcgaacat cgcgcgggtc gggagtccgg tcagctcgtc gtgatacgtg
     2341 aggaacgcga ccttctcgtc tccgcgcttc atctcggtga tgtcgaacat caccccgagc
     2401 gagaacgtgg ggatgccgcg ctcgtcccgg acgatcctcg cctcgtcgtg gatccagacg
     2461 acccgcccgt ccttgcgata cacgcggtag tcgagcgaga aggattcgcc ggtctcgttg
     2521 tgacgctggt tctcggcgat cgcgcgcggg cgatcgtccg ggtggacgat ccgcagccac
     2581 agatccgggt cgcagatcca ctcctccggc gcgtagccga gcatctgctc gacctgcggg
     2641 ctcatgtaga tggtcgacgc cgcgtcgtcg agcgcgtcga tgtaggtgac cgcggggatg
     2701 tgctcgacga tcgagcggta ccggtcacca acgatctcgt ccgcggacgc ctggcgctgc
     2761 cgatgaacgt cggcggcgat cgatccggcc aacacccgca ggagctcgat ctcctcggtc
     2821 ccccacgaac gcccgccgct gcagtcgtcg acgctgagga acccccacca gccttcgtcg
     2881 acggcgatgg gaaccgcgac gaacgacacc gttccctccg ccgcgaggat ccgccgctcc
     2941 gactcgggaa ggtcggtcac ggaagcgctc agcacccgat gatcccccag gacctcgatc
     3001 caccggatga agtcggggga gtacgggtgc aacgtcgtgc tcgggtcgtc gaagaccccc
     3061 cgaacaccgt tccgcctcca ttccgaggcc aggctcatcc agaggcggcc gtcggggtcg
     3121 cggatgttct cgaacaaagc gacccgatcg gcgccgagct ccccccccac gttcgcgagc
     3181 gcatccgaca cctgggcgag cgggggcacg gcgctcgaca cgccgggatc gaccgcctgc
     3241 acggcggccg aggacgagcc ctgcgagacg tccatcctcc ctgctatcgg cggcccccga
     3301 gggccgctga aggagccgcc caggtaggcg tgcctgctac tcttccgggc cgatggccga
     3361 ctacaagcag gttctccacg agcgcctcta cgtgcgcgtt ccgggcacgg acaagctccg
     3421 gaagacggct gccgcccgct tgaagcacct cgtcgccacg ggatggcgcg agaccgagcg
     3481 cgatcgcaag cccgaccaca tctgggtccg gttcgagcgc acgggccacg ccccgatgcg
     3541 ggctcggctc cccaagaagg cgcccgagat ccagatcgaa cgccgacctc gtcgcgacaa
     3601 ccgtggcggc ggcttccgag gcggccggcg ctagccgatc cgcccaagac cggatatctt
     3661 ccggtgaagc gaccctttga ccggacttcc ttcgggtagg atgacgagtc cgcacatcct
     3721 gtgcggttcc tcacccgacg acgatcctgc gaggacccga tgagcgtgga attcctcagt
     3781 cccccttcgg accggcccgg gcaccgcgat ccgcacgccc acgcggccgc ccagccgttc
     3841 acctatccgc tgcaacgccc gtacggggaa ccggactgga cgcgcctcgc cgcgtaccgg
     3901 gacgtcacca cggagcagtg ggaatccgcc cagtggcagc gggcgcacac cgtgaagaat
     3961 ctcaaggagt tcaaggcggc cctcggcgag cacctcgacg acgaactgct cgcggacatc
     4021 gaacgcgacc agcaggagcg ggcgacgatg tccatgctcc tcccgcccca gatgatcaac
     4081 acgatggacg agaccgatct ccgggcggac cccgtccggc tgtacatggc ccccgcgttc
     4141 agcgaccggg atcgggagtt ccccgcccac cccttcgcct cacgggattc cctccacgag
     4201 gccgacatgt gggccgttga gggtctgacc catcgctatc cgaccaaggt tctggccgag
     4261 ttgatcccga cctgcccgca gtactgcggc cactgcacgc gcatggacct ggtgggcaac
     4321 gacacggtgc aggtcctcca gtacaagttc gaggcgaagc agaacgaccg ctgggacgcg
     4381 atgctcgact acctccggcg cacgccgagc gtccgcgacg tcgtcgtgtc cggcggcgac
     4441 atggccaacg tccctatcaa gcgattggag acgttcgtga tgggtctcct cgacatcgag
     4501 aacgtccgcg acatcaggct cgcgacgaag ggactgatgg ggatcccgca gcacttcctg
     4561 caggacgagg tcagggcggc ggcggagcgg atggcctcac gggcgaacga acgcggcgtg
     4621 gacatcgccg tgcacaccca cgtgaacgcg gcgcagcagg tcacgccgct ggtgggccgc
     4681 gcgaccaagg tgttcctcga cgcggggttc cgcgacgtgc ggaaccaggg tgtgctgctc
     4741 cgcggcgtga acaccgctcc gcagcagctc ctcgagctct gtttcatgct cctggaccac
     4801 gcgaagatcc tcccctacta cttctacatc tgcgacatga tcccgaacgc cgagcactgg
     4861 cggacggcgg tgtgggaggt ccaggacctc cagcacgcga tcatgggtta cctgcccggc
     4921 ttcgcgacac cccggatggt cgtggacgtg ccgttcgtcg gcaaacgctg ggcgcatcag
     4981 gtcgccgagt acgaccgtgt ccgcgggatc tccgggtgga cgaagaacta ccggaccggg
     5041 atcgagagcg acgaccccga ggcgttcacc cgccggtacc actactacga ccccatctcg
     5101 accctgcccg aggagggcaa ggcgtggtgg cgggaacagc tcgcgcgagg cgtggacgag
     5161 ctcatcgcgg agctccagcc ccagcacacg tagcccgaac cgcgctgcta gagtccgagg
     5221 cgtgagccgc gtcctcaccg tgctgtcggc ccaggtcgca cccgtcgcct gggaccccgc
     5281 ggcaacgctg gacaagttcg aggatcacgt ccgcgtggcc cgccgcgcgt tcccggacgt
     5341 cgacctgatg gtcttccccg agctctacct gaccgcgatc gacgggttca cctccggagg
     5401 atccgccgac tgggagcgtc gtgtcgccga ggagatcccc ggccccttga ccgaccgcgt
     5461 ggcgaagatc gccgctcgag cgaaacggtg gatcgtagcc gggtcgatca acgagcgacg
     5521 cgggaggaag atccacaaca ccgcgatcgc cttctcgccc gacggggaga tcgcagccgt
     5581 ctaccgcaag ctcttcccgt ggcgcccgtt cgaggacgcc gaccccggcg ccgagccggc
     5641 gccggtgttc acgatccccg ggaagggcgt cgtcggcatg atgatctgct acgacggctg
     5701 gttcccggag gtggcgcgag ggctcgccct ccagggcgcg gagttgatcg cgcagccgac
     5761 cgcgacgacg acgcccgatc gcgaggaaga ggtggtcctc gcgcgagcga acgccatcgt
     5821 gaaccaggtc ttcgtgttca accccaacat ggtctcgacg ttcgggccgg ggcggagcgt
     5881 cagcgtcgac cccgaggggc gggtcctgtt cgaggcgggt gccggtgagg agctgctggt
     5941 ggaggtcgtg gacatggatc gcgtggccga gatccgccgt cgcggcacgc gcggcgtgac
     6001 acggccgtgg cggcacttcc tcgacgggcc ccgggaggtg gtgctccgcc cctaccgggc
     6061 gcgtcgcacc acgtaggcgg cgatccccag ggtcgcgacg gccataccgg ccacgatcca
     6121 ccagatcgag agaccggatc gtgcatccgc tgccggttcg tcctcgccca ccgggtcgat
     6181 cccgacgccg aaccgctccg tcggcctccc gttcccgagg agatcgcagg cgcggccgtc
     6241 gcgcacgatc acggtccacc ggagcgcttc gagcccgggc gacgtgcccg tgagatcctc
     6301 gtcggcgctc aacgtccgac cggggatctg gatcgtcatc gtcgcgccct cgatgagcgg
     6361 tggatcgaac acgttcgcgc cgcgttccgg caggaggtag atctcggtct cgtgggcgac
     6421 ggtcgcgtcg acgcgtacca accggttcac gcccctgtag tacgcgaagg agagcggcgg
     6481 aacgtccggg tccctgatcg cgatgtccac tcggaacggc cgaccgaatg catcgggtac
     6541 ggtgagcggc tccgcgaacg tgagcgtcca gcgagcggag gtgccgagct cgacgatctc
     6601 cccgcgcgcg tccacgaggt cgggcgc
//
LOCUS       DASUVE010000278         5442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_513563, whole genome shotgun sequence.
ACCESSION   DASUVE010000278 DASUVE010000000
VERSION     DASUVE010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513563"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VF036_06005"
     CDS             complement(<1..985)
                     /locus_tag="VF036_06005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6264815.1"
                     /translation="MKEGTGRSAVRRVATARGISLTGSGAAFIVLGVIVYRLTDGSAV
                     WLSLSLLLTMGVQGLVQPIASWLGDRFDRRRVLVVSDLAAGAGFLALAFARTPGQLVT
                     IACVTAILESPVWAVASASIPNLVDEDDLPWANGQVAIGRHLGSFIGPILGTQIVGLM
                     VGDAATDARLLAAGAFVFLLNTASFVLSAWLIGTTPGRFNDERVDVTEHAGVRAGFRY
                     AMSDRVLRSILIGWSILLLGVGLILVAELPYSDVFGRGEEGYGLISALWGGGAAVGAV
                     LAARWLTARREPQTLFVSVLLGGVIMFGIGWSPLWYAALALMVVEGLCEGFA"
     gene            complement(998..2284)
                     /locus_tag="VF036_06010"
     CDS             complement(998..2284)
                     /locus_tag="VF036_06010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6264816.1"
                     /translation="MTRSPKSNVRRLALGRLISVMGGAAAYTALNYTVWERTHSPFMQ
                     ALSLLLTFGVAGLIGPFTGALGDKYNRRTVMLWSEAISAAFFAAMVFAKDPGLLIALA
                     FGSAIAELPFFAASRAAIPNLVESEKDLSWANSLVSMGVHAGIAVGPVIGGGLVAILA
                     PGDTPLPDRLHMAGAVVFGLNAISFLISLAITLTVRGRFEEERSPDAEPEHEGLFAGA
                     AYLWREPVLRRMAFAWLVFVLGMGMGMVADAPLAESFGTGGWGFGLLIACWGTGSVLG
                     TLFGRWMNARTEPLWMVFGAAGVAVSAFGVGLFGLFPLVLVSLLVMGTSDGVSIVAEN
                     GIMQRRTPDAVRSRTMAALEAVLSLGLAVAYLAAGPILRLVEAQTVYVIAGLFAVGAT
                     VVLLPLIRLTSDGGHGRETGPGTVVHEPTTPDRATV"
     gene            complement(2475..4061)
                     /gene="serA"
                     /locus_tag="VF036_06015"
     CDS             complement(2475..4061)
                     /gene="serA"
                     /locus_tag="VF036_06015"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006541009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX6264817.1"
                     /translation="MRVLVTEKLSEAGLELLRERFRVDVREDLAHGDLAGAIAPFDAL
                     VIRSATRVTAEVLDAAPHLKVVARAGIGVDNVDVEAATRRGVMIVNAPQSNTISAAEH
                     TMALLLSQARNVPQADHELHAGRWDRERWQGVELAGKTLGLIGLGRVGTLVASRAQAF
                     GMRVIAFDPYVSGERAKELGVDVMPTLESLLVQSDFVSIHLPGSPDTLGLIGEHQLAM
                     VKHGARLVNTSRGGIVDEEALAKALEDGHLAGAALDVFAVEPATDSPLFGMDQVVVTP
                     HLGASTIEAQDKAGTTVAEMVRLALEGQFVPYAVNVSAGAEVSEAVRPFVGLAERLGS
                     LAAGLADGGVRSIVASYLGRVAEHDTRVLTLAILKGILGRSVNEPVSFVNAPLLARDR
                     GLTVSEMRSTGSQDYVSQISLRAQTESGSEIEVGGTLLGKKNAERVIQVWDFEVEIEP
                     APHMLFFVYEDRPGIIGKVGTILGEHGINIATMEVGRKVAGGDALMALTVDSPVPPDV
                     LRVVAEAIDAHTIRAVTLGT"
     gene            complement(4058..5221)
                     /locus_tag="VF036_06020"
     CDS             complement(4058..5221)
                     /locus_tag="VF036_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX6264818.1"
                     /translation="MDRPEIILAPGPTPIPPEVLLAQGSPLVYHRGPGFGRLMRDVTS
                     RLKELYRTDRADVLLMTSSGTGGLESAVQNVFSPGDEVLVPLAGFFSERWRNLAMAMG
                     LTVRTVEHDWGRRVDPSKVADALAEHPVKAILLTQSETSTGVIQPVEELARVANDVGA
                     LVVADVVSSLGAVPFAFDDWGVDVAVGGSQKALSASPGIAFVAISERAWEAHERAALP
                     RFYFDWSIYRRFAELPDPENPWTPAISVMQGLHAALELYFQDGVDAAYRRHELLSRAV
                     KEGVKALGLDLFGEGLDANWTVTAISAPEGIDADLISDRIRSDFGCILAPGQGPLKGK
                     VFRIGHFGYFSELDIIRGLAALEMTLERLGHPVKRGAAVASAEGVFQEAAVPG"
BASE COUNT          915 a         1941 c         1869 g          717 t
ORIGIN      
        1 tcgcgaagcc ctcgcagagc ccttcgacca ccatcagggc caaggccgcg taccagagcg
       61 gcgaccagcc gatcccgaac atgatgacgc cgcccaggag cacggagacg aagagggtct
      121 ggggctcccg cctcgccgtc agccatcggg ccgccaacac ggcgccgacc gccgctcccc
      181 cgccccacag cgccgagatg aggccgtacc cttcctcgcc ccgaccgaag acatccgagt
      241 acgggagctc cgcaacgagg atcaggccga cgccgaggag caggatcgac caaccgatca
      301 ggatcgatcg aaggacgcgg tcgctcatcg cataccggaa gcccgcacgc acgccggcgt
      361 gctcggtgac atcgacccgc tcgtcgttga atcgcccggg ggtcgtcccg atcagccacg
      421 cggacaggac gaaggaggcc gtgttgagca agaagacgaa cgcaccggcc gcgagcaacc
      481 gcgcgtcggt cgcagcgtcg cccaccatga ggccgacgat ctgggtgccg aggatcggcc
      541 cgatgaagct gccgaggtgc cgcccgatcg cgacctgacc gttggcccac gggaggtcgt
      601 cctcgtccac gaggttcggg atcgaggcgc tcgcgacggc ccacacaggg gactccagga
      661 tcgccgtgac gcatgcgatc gtcacgagct ggccgggcgt ccgagcgaac gcgagcgcca
      721 ggaaacccgc gccggcggcg aggtccgaga cgacaaggac ccggcgccga tcgaaccggt
      781 cgcccagcca gctcgcgatc ggctgcacga gcccctgaac gcccatcgtc agcagcagcg
      841 acagcgagag ccagaccgcc gatccgtccg tgaggcggta gacgatgact ccgaggacga
      901 tgaacgccgc accgcttccc gtcagggaga tcccgcgcgc cgtcgcgacc ctacggactg
      961 ccgagcgacc cgtgccctcc ttcatcgagg gcaagaatca cacggtcgcg cggtccgggg
     1021 tagtcggctc gtgtactacg gttcctgggc cggtctccct accgtgcccg ccgtcggacg
     1081 tgaggcggat gagggggagg aggaccacgg tcgcgcctac ggcgaacagt cctgcgatca
     1141 cgtagaccgt ctgggcctcc accaaacgga ggatcggccc ggcggccaga taggcgacgg
     1201 ccagccccag ggacaggacg gcctccaggg cagccatcgt ccggctccgg accgcgtcgg
     1261 gggtgcggcg ctgcatgatc ccgttctcgg ccacgatcga gacgccgtct gacgtcccca
     1321 tcacgaggag ggacacgagc acgaggggga acagcccgaa caggcccacc ccgaatgccg
     1381 acacggccac cccggcggca ccgaacacca tccacagggg ctccgtgcgg gcgttcatcc
     1441 agcgcccgaa cagggtgccg agcacggagc ccgtccccca gcacgcgatc agtaggccga
     1501 agccccagcc gcccgtcccg aaggactccg cgagcggcgc gtcggccacc atccccatgc
     1561 ccatcccgag aacgaagacg agccacgcga aggccatccg gcgaaggacg ggctcacgcc
     1621 atagataggc ggcgcccgcg aacagcccct cgtgctccgg ttccgcgtcc ggcgaccgct
     1681 cctcttcgaa gcgaccgcgg accgtcagcg tgatggcgag ggagatcagg aaggagatcg
     1741 cgttcaaccc gaagacgacc gcgccggcca tgtgcagccg gtcgggcaac ggcgtgtccc
     1801 cgggtgcgag gatcgccacc aggccgccgc cgatcaccgg accgaccgcg atccccgcgt
     1861 gcacgcccat ggagacgagg ctgttcgccc acgagagatc cttctcggac tcgacgaggt
     1921 tcgggatcgc cgcacgcgag gcggcgaaga acggcagctc ggcgatcgcg gaaccgaacg
     1981 cgagcgcgat caggaggccg gggtccttcg cgaagaccat cgccgcgaag aaggcggccg
     2041 agatggcctc ggaccagagc atgaccgtcc gacggttgta cttgtccccc agcgcgcccg
     2101 tgaacggacc gatgaggccg gccaccccga acgtgagcag cagcgagagg gcctgcatga
     2161 acggcgagtg ggtgcgctcc cacacggtgt agttcagcgc cgtgtacgcg gcggcgccgc
     2221 ccatgaccga gatcagacgg ccgagggcga gacgccgaac gttggacttg ggcgagcggg
     2281 tcatggagcg gtctccttgc gggcgtggtg cggagggtcg acgaagagcg gactacgggg
     2341 cgcgacgcgt gagacgcgtc agagggcccc gtgcactggt cgtttcgtcg tgtaccgctt
     2401 catggtcgtt ccaggctagc acggctgtcc acccccaccg ccgaggctcg gcaaccttcg
     2461 gtcaagaggt tcggtcaggt gccgagcgtg acggcgcgga tcgtgtgcgc gtcgatcgcc
     2521 tcggccacga cgcgcagcac gtcgggcggg acgggtgagt ccaccgtgag ggccatcaag
     2581 gcgtcgcccc cggcgacctt ccgcccgacc tccatcgtgg cgatgttgat gccgtgctcg
     2641 ccgaggatcg tgccgacctt cccgatgatc cccgggcggt cctcgtagac gaagaagagc
     2701 atgtgggggg cgggctcgat ctcgacctcg aagtcccaga cctggatcac gcgttcggcg
     2761 ttcttcttgc cgaggagcgt gccgccgacc tcgatctcgg atccggactc cgtctgggcc
     2821 cgcagggaga tctggctcac gtagtcctgc gacccggtcg agcgcatctc gctgaccgtg
     2881 agccccctgt cgcgcgcgag caacggcgcg ttcacgaagg acacgggctc gttcacggag
     2941 cgtccgagga tccccttcaa gatcgcgagc gtgagcaccc gtgtgtcgtg ttccgccacg
     3001 cgacccaggt aggaggcgac gatcgatcgc accccgccgt ccgccagacc cgctgccaac
     3061 gatccgaggc gctccgcgag accgacgaag ggtcggacgg cctcgctcac ctcggcgccg
     3121 gcggagacgt tcacggcgta gggcacgaac tgaccctcga gcgcgaggcg caccatctcg
     3181 gcgaccgtcg tgccggcctt gtcctgcgcc tcgatcgtcg atgccccgag gtggggggtg
     3241 acgaccacct ggtccatccc gaacagcggc gagtcggtgg cgggctccac cgcgaagacg
     3301 tcgagggcgg cgcccgccag gtgaccgtcc tcgagcgcct tcgcgagcgc ctcctcgtcg
     3361 acgatgcccc cgcgcgatgt gttcacgagc cgggcgccgt gcttgaccat cgcgagctgg
     3421 tgctccccga tcagtcccag ggtgtccggg gagcccggga ggtggatcga gacgaagtcg
     3481 gactgcacga ggagcgactc gagggtgggc atcacgtcca cccccagctc cttcgcccgc
     3541 tcgcccgaga cgtacgggtc gaacgcgatc acgcgcatcc cgaaggcctg ggcgcgcgaa
     3601 gccacgagcg tcccgacccg cccgagaccg atcaacccga gggtcttgcc cgcgagctcg
     3661 acgccttgcc atcgttcccg gtcccaacgc ccggcatgga gctcgtggtc cgcctgcggg
     3721 acgttccgag cctgcgagag caacaacgcc atcgtgtgct ccgcggcgct gatcgtgttc
     3781 gactgggggg cgttcacgat catcacgcca cggcgcgtgg cagcctcgac gtcgacgttg
     3841 tcgacgccga tgccggcgcg cgccaccacc ttcaggtggg gcgccgcgtc gaggacctcg
     3901 gccgtcacgc gggtcgcgct ccggatcacg agcgcgtcga acggcgcgat cgcgccggcg
     3961 aggtccccgt gcgcgaggtc ctcgcgcacg tcgacccgga accgttcgcg gaggagctcg
     4021 aggcccgcct cggagagctt ctcggtgacg aggaccctca tcccgggacg gcggcctcct
     4081 ggaagacccc ctccgccgac gccacggccg cgcctcgctt cacgggatgg ccgaggcgct
     4141 cgagggtcat ctcgagggcg gcgagccctc ggatgatgtc gagctcgctg aagtaaccga
     4201 agtgcccgat gcggaacacc ttccccttca agggcccctg accgggggcg aggatgcagc
     4261 cgaagtcgga gcggatccga tcgctgatca ggtccgcgtc gatcccctcg ggggcgctga
     4321 tcgccgtgac cgtccagttc gcgtcgaggc cctcaccgaa cagatccaga ccgagtgcct
     4381 tcacgccctc cttcaccgcg cgggacagga gctcgtgacg gcggtacgcg gcgtcgaccc
     4441 cgtcctggaa gtacagctcg agggccgcgt ggagcccctg catcacgctg atcgccggcg
     4501 tccacgggtt ctccgggtcg gggagctcgg cgaaccggcg atagatcgac cagtcgaagt
     4561 agaagcgggg cagcgcggcc cgctcgtgcg cctcccaggc acgctcgctg atcgcgacga
     4621 acgcgatccc ggggctcgcc gacagcgcct tctgcgaacc gccgaccgcc acgtcgaccc
     4681 cccagtcgtc gaacgcgaac ggcaccgcgc cgaggctgct cacgacgtcg gcgaccacga
     4741 gcgcgccgac gtcgttcgcg acccgggcga gctcctcgac cggctggatc acgcccgtcg
     4801 acgtctccga ctgggtgagg aggatcgcct tgacgggatg ctcggcgagc gcgtccgcga
     4861 ccttcgacgg gtccaccctt ctgccccagt cgtgctcgac cgtccggacg gtgagaccca
     4921 tcgccatggc gaggttgcgc cagcgctccg agaagaaccc ggccaagggg accagcacct
     4981 cgtcgccggg cgagaagacg ttctggacgg ccgactcgag gcctccggtg cccgacgagg
     5041 tcatcagcag gacgtccgcg cgatccgtgc ggtacagctc cttcaggcgg gaggtcacgt
     5101 cgcgcattaa gcgaccgaaa ccgggccctc ggtgatacac gagcgggctc ccttgggcga
     5161 ggagcacttc cggcgggatc ggggtgggac cgggcgccag gatgatctcg ggcctgtcca
     5221 tgcggtctcc tgtcgtgtcg gcggttcgcg acgacgaacg cgaggactca tcgaccgcgc
     5281 aaggagctga tcggcaatgg acaggcccga gatcatcctc gctccgggac cgacgccgat
     5341 cccgccccag gtcctgctcg cacagggaag cccgctcgtt taccaccgcg ggccggggtt
     5401 cgggaggctc atgcgggacg tcgcgggtcg cctgcgtgac ct
//
LOCUS       DASUVE010000279         2924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_324054, whole genome shotgun sequence.
ACCESSION   DASUVE010000279 DASUVE010000000
VERSION     DASUVE010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324054"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /gene="deoA"
                     /locus_tag="VF036_06025"
     CDS             <1..805
                     /gene="deoA"
                     /locus_tag="VF036_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012800.4"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEX6264819.1"
                     /translation="LRDATATVPSIPLIAASVMSKKLAVETDLILLDVKTGSGAFMKT
                     LEDARALAERCLALATGWGRAASAAVTDMSQPLGDAVGNALDIAETVAVLRGDSGGRL
                     REAALLFAARALEHTRGIGHEEALAQAVGAIGSGDALERFRRMIEAQGGDPHVVDDPA
                     GVLPAAPVREPLLADRTGVLAAVDAEAIGLASVGLGAGRRSKGDPIDPAVGIVVHPKI
                     GDRLEAGTPIGEVHARDRDAAAEAGRRVLGAMDVTDGAVAPPPLIYDWM"
     gene            809..1474
                     /locus_tag="VF036_06030"
     CDS             809..1474
                     /locus_tag="VF036_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX6264820.1"
                     /translation="MGNFTALGLRLAAYMLLSLFIGMTLREYARARAAAALGDPTPRL
                     WGRITLRPKPWFDPFGSGFIASMVALLWTVQAFMMPAAYAKPAPIDTNYLRRHPRDVV
                     LVSLAGPVASLALAALGGIVIRAGVGGQVGLAILTFVFTNVSLMVFHLLPIPGLDGGR
                     LLALLLPPHAREVFRNADRYLALIVLVVLFLFTFLLGVARLLAGAVCDALSGVDCSGL
                     FRF"
     gene            1525..2568
                     /gene="trpS"
                     /locus_tag="VF036_06035"
     CDS             1525..2568
                     /gene="trpS"
                     /locus_tag="VF036_06035"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX6264821.1"
                     /translation="MTSSSPSDDASGSEGVEPRRGRVLTGFRPTGDLHVGHWAGNVGN
                     AVRLQDEGYECYYFVANWHMLTTHYDRTAELGSLVEAIVLDLLACGIDPERSTLYLQS
                     DLPEVAEMALLLGMIAPLGWLERVPTYKERLRDMAERDVANLGLLGYPVLQTVDISIV
                     RGELVPVGEDQVSHLEISREIVRRFNRLFGEILVEPQPLLSDFPLVPGSDGRKMSKSL
                     DNAIGLVDDPDTIRAKVRSFITDPQKVRLGDPGRPVICPIYALHTRFSPDIVDWTRDH
                     CRSGELGCVDCKTNLADRLIEELRPIRERRLELASEPGLVWKVLAEGRERVGPVVRET
                     MDAVRDAMGFSRP"
     gene            2565..>2924
                     /locus_tag="VF036_06040"
     CDS             2565..>2924
                     /locus_tag="VF036_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX6264822.1"
                     /translation="MTSEGAGAFAVELPAFTGPFRLLAELILEQKVDVCDVSVATVTD
                     GFLAYAKDADHWDLEEVTWFLAMCAVLLELKVGRLMPRHTETDEDDLLGASPDLAYAR
                     SMELAAFRKVAVELARRL"
BASE COUNT          383 a          998 c         1052 g          491 t
ORIGIN      
        1 gttgcgcgac gccacggcga cggtcccgtc gatcccgctg atcgcggcca gcgtgatgtc
       61 caagaagctg gcggtggaga cggacctcat cctcctggac gtgaagacgg gctcgggcgc
      121 cttcatgaag acgctcgagg acgcgcgggc gctggcggag cgatgtctcg cgctcgccac
      181 cggctggggc cgtgcggcga gcgccgcggt gacggacatg tcccagccgc tcggtgatgc
      241 ggtcgggaac gccctggaca tcgccgagac ggtggccgtg ctccgaggag actcgggcgg
      301 tcgtctccgc gaagcggcgc tgctcttcgc cgcccgggcc ttggagcaca cgcgtgggat
      361 cggtcatgag gaggccctcg cgcaggcggt cggtgcgatc ggctcgggcg acgcgctcga
      421 gcggttccgt cggatgatcg aagcgcaagg gggagatcct cacgtcgtgg acgacccggc
      481 cggcgtgctc ccggccgctc ccgtgcgcga gccgctgctc gcggaccgta ccggtgtcct
      541 cgcggccgtc gacgcggagg cgatcgggct cgcgagcgtc gggctgggag ccggtcggcg
      601 gagcaaaggt gaccccatcg accccgccgt cggtatcgtg gtgcatccga agatcggcga
      661 ccggctggag gccggaacgc ccatcgggga ggtgcacgcg cgcgatcgcg acgccgcggc
      721 ggaggccggg cgtcgtgtcc tcggcgcgat ggacgtcacc gatggtgcgg tcgcgccccc
      781 gccgctcatc tacgactgga tgtgagggat ggggaacttc acggcgctcg ggctccggct
      841 cgcggcctac atgctcctat ccctgttcat cgggatgacg ctccgcgagt acgcgcgtgc
      901 ccgggcggcc gcggcgctcg gcgaccccac cccgcggctg tggggccgga tcacgctccg
      961 accgaagccg tggttcgacc cgttcgggag cggcttcatc gcctcgatgg tggcgctgct
     1021 ctggaccgtg caggcgttca tgatgccggc ggcgtacgcg aaaccggccc cgatcgacac
     1081 gaactacctg aggcgccacc cccgtgacgt cgtcctcgtc tcgctcgcgg gtccggtcgc
     1141 gtcgctcgcg ctggccgcac tcggggggat cgtgatccgt gccggcgtcg ggggtcaggt
     1201 ggggctcgcc atcctgacgt tcgtgttcac gaacgtctcg ctcatggtgt tccatctcct
     1261 gccgatccct gggctcgacg gcggccggct cctcgcgctg ctgcttcccc cacacgcgcg
     1321 cgaggtgttc cggaacgcgg atcggtacct cgcgctgatc gtcctcgtgg tgttgttcct
     1381 gttcacgttc ctcctcggcg tcgccaggct ccttgccggc gcggtctgcg acgccctctc
     1441 gggcgtggac tgctcgggcc tcttccgctt ctgagtcggg cgttcccggc cgtgggaggc
     1501 ggggccggta tcatcggccg catcgtgacc tcttcgtccc cgtccgacga tgcgtccgga
     1561 tcggagggcg tggagccgcg ccgcggccgc gtcctgaccg gcttccgtcc cacgggcgat
     1621 ctccacgtcg gccactgggc gggcaacgtg ggcaatgcgg tccgactcca ggacgagggg
     1681 tacgagtgct actacttcgt cgcgaactgg cacatgctca cgacgcacta cgatcgcacc
     1741 gcggagctcg gatcgttggt cgaggcgatc gtgctcgatc tcctcgcgtg cggcatcgac
     1801 cccgagcgtt cgaccctcta cctgcagtcg gatctccccg aggtcgcgga gatggcgctg
     1861 ctcctgggga tgatcgcacc cctcggctgg ctcgagcgtg tgcccaccta caaggagcgg
     1921 ttgcgcgaca tggccgagcg cgacgtcgcg aacctcgggc tgctcggata cccggtgctc
     1981 cagacggtgg acatctcgat cgtccgcggg gagctcgtgc cggtgggcga ggaccaggtg
     2041 tcccacctcg agatctctcg ggagatcgtg cgccgattca accggctctt cggcgagatc
     2101 ctcgtcgagc cccagccgct cttgtcggac ttcccgctcg tgccggggtc cgacggccgg
     2161 aagatgtcca agtcgctcga caacgcgatc ggcctggtcg acgatcccga cacgatccgg
     2221 gcgaaggtcc ggtcgttcat caccgacccg cagaaggtgc gcctcggtga ccctggcagg
     2281 ccggtgatct gcccgatcta cgcactgcac acgcggttct cacccgacat cgtcgattgg
     2341 acgcgcgatc actgccgctc cggggagctc ggctgcgtgg actgcaagac gaacctcgcc
     2401 gatcgcctca tcgaggagct ccggccgatc cgggaacgac ggctcgagct ggcctcggag
     2461 ccggggttgg tgtggaaggt cctggccgag gggagggagc gcgtcggacc cgtggtgcgg
     2521 gagacgatgg acgccgtgcg cgatgcgatg gggttctcac gaccgtgacg agcgaggggg
     2581 cgggcgcgtt cgcggtcgaa ctgcccgcgt tcaccggacc gttccggctg ttggccgagc
     2641 tgatcctgga gcagaaggtc gacgtgtgcg acgtgtcggt cgcgaccgtg accgacggct
     2701 tcctcgccta cgcgaaagac gccgaccact gggacctcga ggaggtcacg tggttcctcg
     2761 cgatgtgcgc cgtgctcctc gagctgaagg tcggccggct gatgccgagg cacaccgaga
     2821 cggacgagga cgatctcctc ggtgcctccc cggacctcgc ctacgccagg tcgatggagc
     2881 tggccgcgtt ccggaaggtc gcggtcgagc tcgctcgccg tctc
//
LOCUS       DASUVE010000280         3969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_324445, whole genome shotgun sequence.
ACCESSION   DASUVE010000280 DASUVE010000000
VERSION     DASUVE010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324445"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VF036_06045"
     CDS             complement(<1..181)
                     /locus_tag="VF036_06045"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEX6264823.1"
                     /translation="MSSVTFEAVVESARGGGHVVEVDRALAGRIGAKHMTRVRGTLDG
                     TEYRSNVASMGGRLVL"
     gene            complement(178..939)
                     /locus_tag="VF036_06050"
     CDS             complement(178..939)
                     /locus_tag="VF036_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6264824.1"
                     /translation="MVTGASSGIGEAAAFAFAKRGARVVLAARRTERLDAVVERIEKA
                     GGRALAATCDVTVPDQLRTLRAVTEEAFGPTDVLVNSAGMRGGGEYTKLDYERIEEHV
                     RLNVLGVMFATRAFLPGMLARGIGHVVNIGSLAGRFATPGNPVYGATKHAVVAFSEAM
                     NYETEGRNVHVTSVNAGFVDTEGFPQGDLPNWAVLKMSTVTDAIVKVVRGDIAPEYSV
                     PRWLSPFQMFRVATPPLYRWGIRRVRRNRRGTSRP"
     gene            993..1820
                     /locus_tag="VF036_06055"
     CDS             993..1820
                     /locus_tag="VF036_06055"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEX6264825.1"
                     /translation="MDVIGAHAKRLPVLDERWEARPYPREELARALAAGGVAGPAGHP
                     HHNVFGNIRKLVEHDPDKLFGLSRMPEPFGFEDIVRLVGEEAGVPIDALAIEGSPDID
                     AAAVLDACERMGERLAAAARRGERVLFATGHPGDLDPLYGAIADLAAARGARIVRPAE
                     GISWQEPGHRWTISFHGAVGMVTDEERPRHSHRPDAMEIMLTAGRADLVVADHGFAGA
                     AIDAGVETLSIADVNDPALIVAKAQGRTEIVVVLDDHVPPENYWPCFQAVAGRFDPS"
     gene            complement(1817..2122)
                     /locus_tag="VF036_06060"
     CDS             complement(1817..2122)
                     /locus_tag="VF036_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264826.1"
                     /translation="MTEARRALLRKLEHADAFPLVALQALRELRTDLDAVEAEAILRA
                     RELGASLEDIAEALEITRQGVSYRLKVLAENEHEDDDSVEDEIVDVREPDPRGARSD"
     gene            2571..>3969
                     /locus_tag="VF036_06065"
     CDS             2571..>3969
                     /locus_tag="VF036_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264827.1"
                     /translation="MTGAFADQIVADADALILGSPHGNNVTASQAVGTTHAYDFSAAI
                     RETGNATDNVFPGTVSVSIAKSGDWVGSGGTGGPWIFTGYESGQAGTIDITVPSDACD
                     VTETMSVTLTAGTSSNGKILNPNSRVLTYVITGTGTCAPSDSTAPVIGYVLTPAVPDG
                     ANGWYKSDVPLTWTVTENESPASLIKTGCVDQSITSDQGPTVYSCAATSEGGSAGPVS
                     VSVKRDATAPTINATLSPAADAGTGWYNIATGHPTVSFTCSDATSGIPTGTCPGAHTF
                     NADAENQSFSANVADAAGNTTTAGVSNVDVDLTAPGISFVGAAPAPNGAGWNNTDVVL
                     TWACTDTTSGVVDATVQETIDTDGSSQTATGTCYDNAGNSNSLGSGDVDRDTIDPTIV
                     AGLAPSDPAATGWYNAATGAPTVSFSCDDDRSGIPAGTCPVSHTFGNGEDQSFSATVF
                     DAAGTESDSAGVSNVD"
BASE COUNT          668 a         1354 c         1312 g          635 t
ORIGIN      
        1 ccaggacgag gcggccgccc atcgacgcga cgttgctccg gtactcggtg ccgtcgagcg
       61 tcccgcgcac gcgcgtcatg tgcttcgcgc cgatccggcc ggccagcgcg cgatccacct
      121 ccaccacgtg cccgccgccc cgcgcggact ccaccacggc ttcgaacgtc acggagctca
      181 tggccgtgac gtgccgcgtc ggttccggcg gacgcgtcgg atcccccatc ggtacagggg
      241 tggcgtcgcg acccggaaca tctggaaggg gctcagccat cgtgggaccg agtactcggg
      301 agcgatgtcc cccctgacga ccttcacgat cgcgtcggtc acggtactca tcttgagaac
      361 ggcccagttc ggcaggtccc cttgcgggaa cccctcggta tcgacgaacc ccgcgttcac
      421 cgaggtgacg tgcacgttgc gcccctcggt ctcgtagttc atcgcctcgg agaacgccac
      481 cacggcgtgc ttcgtggccc cgtacaccgg gttgcccggc gtcgcgaagc gtcccgccag
      541 cgacccgatg ttcacgacgt ggccgatgcc acgggcgagc atccccggga ggaacgcgcg
      601 cgtggcgaac atcacgccga ggacgttcag ccggacgtgc tcctcgatcc tctcgtagtc
      661 gagcttcgtg tactctccgc cgccccgcat gccggcgctg ttcacgagca cgtcggtcgg
      721 accgaaggcc tcctccgtca ccgcgcgcag ggtacggagt tggtcgggca cggtgacgtc
      781 gcaggtggcg gcgagggcac ggccgcccgc cttctcgatc cgctcgacca cggcgtcgag
      841 tcgttcggta cggcgcgccg cgaggacgac gcgggcacct cgcttcgcga acgcgaacgc
      901 cgccgcctcg ccgatcccgg acgatgcgcc ggtgaccacg aacactgctc cgtcgatgcg
      961 caggaagaac cccctcgtac gatggtggtg ccttggacgt tatcggagcc catgccaagc
     1021 gtctgccggt gctcgacgaa cgctgggagg cgcgcccgta cccccgggag gagttggcgc
     1081 gggcactcgc ggccggcggg gtcgccggcc cggcgggtca cccgcaccac aacgtgttcg
     1141 ggaacatccg gaagctcgtc gagcacgatc ccgacaagct cttcggcctc tcgcgcatgc
     1201 cggagccgtt cgggttcgag gacatcgtgc ggctggtggg cgaggaggcc ggggtgccga
     1261 tcgatgccct cgccatcgag ggatcgcccg acatcgacgc ggcggccgtg ctggacgcgt
     1321 gcgagcggat gggggagcgg ctggcggcgg cggcgcgccg cggcgaacgc gtgctcttcg
     1381 ctaccggcca tccgggagat ctcgacccac tgtacggcgc gatcgccgac ctcgccgcgg
     1441 cgcgcggcgc gcggatcgtc cggccggccg aggggatctc ctggcaggaa ccgggtcatc
     1501 gctggacgat ctccttccac ggagcggtgg ggatggtcac ggatgaggag cgcccccgac
     1561 actcgcatcg cccggacgcg atggagatca tgctgacggc cgggcgcgcg gacctcgtcg
     1621 tcgccgatca cggcttcgcc ggcgccgcga tcgatgccgg cgtggagacg ctctcgatcg
     1681 ccgatgtgaa cgatcccgcg ctgatcgtcg cgaaggcgca aggccggacc gagatcgtgg
     1741 tcgtcctcga cgatcatgtt ccgcccgaga actactggcc gtgcttccag gcggtcgcgg
     1801 gacgcttcga tcccagctag tcgctgcgcg ccccgcgggg atccggctcc ctgacgtcga
     1861 cgatctcatc ctcgaccgag tcgtcgtcct cgtgttcgtt ttcggcgagc accttcaatc
     1921 tgtaggagac gccctgacgc gtgatctcca gcgcttcggc gatgtcctcg agtgaggcgc
     1981 cgagctcgcg agcacgcaag atcgcctcgg cttccaccgc atcgaggtcg gtgcggagct
     2041 cgcggagcgc ctgcagagcg accaagggga aggcgtccgc gtgctcgagc ttgcgcagaa
     2101 gggcacgtcg agcctctgtc ataccgcgga gcatacgaga gcggtatgca agaaacaagg
     2161 accggcgtat gcaggacggc ttgcctagcc tagttccgcg tatccccgag aagccctttc
     2221 ctgagcacga aattcgtttc gcccaggaaa ggaggcttga tgtcggccgt tcgggggtat
     2281 tgacgggcgt cccccatctc acctacgctc ctccatccac gcaattgcct acagcagtcg
     2341 cggatggatg ggatacctgt cggcgcgtcc ggagggggct cttcggagcg gggcgcgccg
     2401 accggttcaa ctgagaggac aggtggatgc gtcggaagaa agcagcttcg acgggttcgg
     2461 gcacgcagct aggcgatcgg tggtccttcg ggccgcgtcg ttcccacgca gtccggggca
     2521 accgcacatc actccttgcc gcggcagtgt cgttgttcat ggtgctcggg atgaccggcg
     2581 ctttcgccga tcagatcgtt gcagacgctg acgccctcat cctcggctcg ccgcatggga
     2641 acaacgtgac agcgtcgcaa gccgtcggca ctacgcacgc atacgatttc agcgcggcca
     2701 tcagggaaac cggcaatgcc acagacaacg tgtttccggg caccgtttcc gtctccatcg
     2761 ccaagtcggg ggactgggtg ggctcaggag gcaccggcgg accctggatc ttcacggggt
     2821 acgaatcggg tcaggctggg accatcgata tcaccgttcc gtccgatgcg tgtgacgtta
     2881 cagagacgat gtccgtaacc ctcacagcgg gcacgtcgag caacggcaag atcttgaacc
     2941 cgaatagccg cgtgttgacc tacgtgatca ccggaaccgg cacgtgtgcg ccgagcgact
     3001 cgacggcacc cgttatcggc tacgtgctca ctccggccgt cccggacggc gccaacggct
     3061 ggtacaagag cgatgttccg ctgacgtgga ccgtgaccga gaacgagtca cctgcttcgc
     3121 tgatcaagac cggatgcgta gaccagtcga tcacatccga tcaaggcccc accgtctatt
     3181 cctgcgcagc aacgagcgaa ggcggtagtg cgggtccggt gagtgtgtca gtcaaacggg
     3241 acgcgaccgc tccgacgatc aacgcgaccc tatccccggc agctgatgcc ggcactggct
     3301 ggtacaacat cgcgacgggc caccccacgg tctccttcac atgcagcgat gccacatcgg
     3361 ggatccccac ggggacatgc cccggagccc acacgttcaa cgcagacgca gagaaccagt
     3421 ccttcagcgc gaacgtcgcg gatgccgccg gcaacaccac cactgcgggc gtctcgaacg
     3481 tcgatgttga cctgacggcg ccggggatct cgttcgtcgg agcagcgcct gcccccaacg
     3541 gggcggggtg gaacaacacc gacgtggttc tcacgtgggc ctgcaccgac acgacgtcag
     3601 gtgtcgtgga cgcgaccgtc caggaaacga tcgatacgga tggatcgagt cagacggcga
     3661 ccggcacctg ctacgacaac gctgggaaca gcaactcatt ggggtcgggt gacgtcgacc
     3721 gcgacacgat tgaccccacc atcgtcgccg gcctggctcc gtccgaccca gccgcaacgg
     3781 gttggtacaa cgccgcgaca ggtgctccaa cggtctcgtt ctcctgcgac gacgaccggt
     3841 cgggtatccc cgccggcaca tgcccggtct cccacacctt cgggaacggg gaggaccaat
     3901 cattcagcgc gaccgtgttc gatgccgccg ggactgagtc cgacagtgcc ggagtctcga
     3961 acgtcgatg
//
LOCUS       DASUVE010000281         5112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_515925, whole genome shotgun sequence.
ACCESSION   DASUVE010000281 DASUVE010000000
VERSION     DASUVE010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515925"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="VF036_06070"
     CDS             complement(<1..1449)
                     /locus_tag="VF036_06070"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX6264828.1"
                     /translation="MRSRRGPTLDPLERLERVLRVSDVTLAHLSVDDLLDELLNRVRE
                     ILDTDTAAVLLLDEGRNELVARAAKGIEEEVERGVRIPVGKGFAGRIAAERKPVVLYR
                     VDHSTVLNPILFQKGIRSLLGVPLIAQGEVVGVMHVGTKTRRRFTRDEAELLQLVADR
                     VALSLRVRLSDRSRVVIEAFQRTFLPEVLPFLPGLRVATRYLPAATAVGVGGDWFDTF
                     VRPEGDIVFVIGDVAGHGLPAASLMGKMRNALRAHALMGGTALDILLRADEFHRHFGE
                     GELVTLLIGLLARDLRTFRYVSAGHPPPLVVGPNGTGAAFASDHRANPPLGLPQPPTF
                     VEEEVAIELGTSILLYTDGLIERREESLAVGLERLRSTAERALDGPPTNEAVTELLEG
                     MIDGPRPTDDVALMVLNRDAIPATELEFAVEARARSLLMVRRAVSRWLEDLEVPDGLA
                     KEIVMAVHEASANVVEHAYGPAGGTITIMATFE"
     gene            complement(1490..2173)
                     /locus_tag="VF036_06075"
     CDS             complement(1490..2173)
                     /locus_tag="VF036_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264829.1"
                     /translation="MPEELNTEIAETLVSTESGSLTKTRWQQTVEIIEATLLAIVAVA
                     TAWSGYQAARWDGRQAELYGESSTMRNEADQALTLGGQQRLLDITTFNTWIEARNEGL
                     DDLAALYERRFSPEFKVAFDAWIALDPFSNPDAPPGPGFMPEYENHLLDEGASLNDAA
                     DRTFDEGTAARHQADEYIRTTVVLATVLFLLALSQRFLIRRVRIAVVVLAAALMVFGL
                     ATIMTFPRL"
     gene            2259..2534
                     /locus_tag="VF036_06080"
     CDS             2259..2534
                     /locus_tag="VF036_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264830.1"
                     /translation="MDDRDLIAKAERALTEIKVNSGLSERHEAVLAALRIRLTGSAGK
                     SLEEMIEAADDAPPDRLGEAITKAEDTGKPSLDDLLGKEPKKPDWPG"
     gene            complement(2540..>5112)
                     /locus_tag="VF036_06085"
     CDS             complement(2540..>5112)
                     /locus_tag="VF036_06085"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264831.1"
                     /translation="WHQGRCLAYGEGVAFWALAEMIRGRAGIAEDEESSTASAKLAQA
                     IEEHIPDADERRFVRPRLGHLLGLEEGGVGDQENLFAACRIFFERIADRGPSIMVFED
                     IHWADSALLDFIEYLVEWSRDRPIFVLTLARPELAERRPSWGAGTRNFTSIFLEPLDS
                     DEMGSLLSDPIPGLPDDVRKRILERAEGIPFYAVETVRMLIDRGILVRENGNFRLEGS
                     VETLEVPETLQALIAARLDGLDPAERRLLQDASVLGRTFTLQGLVAITGLPDEEVTSL
                     LGSLVRKEVLSISIDPLLSDRGQYGFLQDLVKKVAYDTMSKHERKARHLAAATYLRSA
                     VDEDEIVEVVASHLLDAYRAAPDEEDAERIRVEALEMVRKASERAASLGASEEAQRYA
                     ERAIELTDDPLAVAELHERAGMMANVRVDVDSASAHFRAAIELFEAEGATHPAARVSA
                     RLGEVVWSQGKGIDALESMTASYRVLADEEPDADLAQLAAQLGRFTFFNGETERAMDW
                     IERSLEIVEALDLPEILSEGLNSKALILQTFGRASEAMGLMRHALTIALEHDKPSAAL
                     RAYNNLVDLCDYEDRYAEADRMGDEGLTLSRRVGNWFWESTFLGQAYPKFALGRWDDL
                     MASLAEIPPDEFARSRSAFNQGYVSFGTAVEVHRGDLAAALRRLERFADLQTSADVQE
                     VSEYACGAATLRLAEGDPKEALRLAEIGLEGREALGLSHSCVKEGVVTGLEAALALGD
                     QTRVHEILTTVRTDPIGRRTTFFLAHGARIEARSPDTDDEEAERLFRSSIRSFREIAF
                     PFWLAVALLEYGEWLESRGRGPDVAPLVLEARSIFEGLGAQPWLDRADRVSQGSSVIT
                     "
BASE COUNT          792 a         1790 c         1742 g          788 t
ORIGIN      
        1 ctcgaacgtc gccatgatcg tgatggtacc ccccgccggt ccgtatgcgt gctcgacgac
       61 gttcgccgac gcctcgtgca cggccatcac gatctccttc gccaggccgt ccggcacctc
      121 gagatcctcg agccagcgcg agacggcccg gcggaccatc agaagcgagc gagcacgagc
      181 ctcgaccgcg aactccagct cggtcgccgg gatcgcgtcg cggttgagga ccatcagcgc
      241 gacgtcatcc gtcggtcgtg gaccgtcgat catcccctcc aggagctcgg tgaccgcctc
      301 gttggtcggc ggtccgtcca gggcccgctc cgcggtcgag cggagccgct cgagccccac
      361 ggcgagcgat tcctcccgtc gctcgatcag gccgtccgtg tagagcagga tcgacgtgcc
      421 cagctcgatc gccacctcct cctcgacgaa cgtcggcggt tgcggcagac cgagcggtgg
      481 gttcgcccga tggtcggacg cgaacgcggc tccggttccg ttcggaccga cgaccagcgg
      541 tggcgggtgc ccggccgaga cgtatcggaa ggtccgcaga tcgcgggcga gcaggccgat
      601 caggagcgtc accagctcgc cctccccgaa gtgccggtgg aactcatcgg cccgcaggag
      661 gatgtcgagg gccgtcccgc ccatgagcgc gtgcgcgcgg agggcgttgc gcatcttgcc
      721 catcagcgat gccgccggca ggccgtggcc ggccacgtcg ccgatcacga agacgatgtc
      781 gccctcggga cggacgaacg tgtcgaacca gtcgccgccc acgccgacgg cggtcgcggc
      841 gggcaggtag cgcgtcgcca cacgcaggcc cggcaggaac gggaggacct ccgggaggaa
      901 cgtccgctgg aacgcctcga tcacgacgcg ggaacgatcc gacagacgaa cgcggagcga
      961 gagcgcgacc cggtcggcga cgagctggag gagctcggcc tcgtcgcgcg tgaaacgccg
     1021 gcgcgtcttc gtccccacgt gcatcacgcc gaccacctcg ccctgcgcga tgagcggcac
     1081 accgaggagc gagcggatgc ccttctggaa caggatcggg ttcagtacgg tgctgtggtc
     1141 cacccggtag aggacgaccg gcttccgctc ggccgcgatg cgaccggcga atcccttccc
     1201 cacggggatg cggacaccgc gttccacctc ctcttcgatc cccttggcgg cccgggccac
     1261 cagctcgttc cgcccctcgt ccagcaggag gacggcagcc gtgtcggtgt cgaggatctc
     1321 ccgcacccgg ttgagcagct catccaggag atcgtcgacc gagaggtggg cgagggtgac
     1381 gtcgctgacg cgcaggaccc gctcgaggcg ttccagaggg tcgagcgtgg gacctctgcg
     1441 agagcgcacc cacctactgt gcgtccggct cccctcaacc agcaacctgt cagaggcgcg
     1501 ggaaggtcat gatcgtggcg aggccgaaca ccatcagggc ggccgcgagg accacgacgg
     1561 cgatccgcac ccgccggatc aggaaccgtt gggagagggc gagcaggaac agcacggtcg
     1621 cgagcaccac ggtcgtgcgg atgtactcgt cggcctgatg gcgtgccgcc gtcccctcgt
     1681 cgaaggtccg gtcggctgcg tcgttcagac tggcgccctc gtcgaggagg tggttctcgt
     1741 attcgggcat gaatcccgga ccgggtggcg cgtcggggtt cgagaacgga tcgagcgcga
     1801 tccaggcgtc gaacgcgacc ttgaactccg ggctgaacct gcgctcgtag agcgcggcga
     1861 ggtcgtcgag tccctcgttc cgcgcctcga tccacgtgtt gaacgtggtg atgtcgagca
     1921 atcgctgctg gccacccagg gtgagcgctt ggtccgcctc gttgcgcatc gtcgaggact
     1981 cgccgtagag ctccgcttgg cgcccgtccc acctcgctgc ctggtagcca ctccatgcgg
     2041 tcgcgacggc gacgatcgcg aggagggtcg cctcgatgat ctcgacggtc tgttgccacc
     2101 gggtcttcgt cagactgccg ctctcggtcg agaccagggt ctcggcgatc tcggtgttga
     2161 gttcttccgg catcggcacc tcgtgcgcag gaggctgcga gtctaggcgc ggctcggccc
     2221 ggtgcgtcta agagaactcc catggcacac tgtcctccgt ggacgatcgc gacctcatcg
     2281 ccaaggcgga gcgagccctg accgagatca aggtcaacag tgggctctcg gagcggcacg
     2341 aggcggtgct tgcggcgctt cgcatccggc tgacaggaag cgccgggaag tcgctcgaag
     2401 agatgatcga ggcggcggac gacgcgccgc cggaccggct cggcgaggcg atcacgaagg
     2461 ccgaggacac ggggaagccg tcgttggacg acctgctggg caaggagccg aagaagcccg
     2521 attggcccgg ctgagccggt caagtgatga cggatgaacc ctggctgacg cggtccgcgc
     2581 ggtcgagcca cggctgcgcc ccgagacctt cgaagatcga tcgggcctcc aggacgagcg
     2641 gggcgacgtc cggcccgcgg ccgcgggatt cgagccactc gccgtactcc aggagcgcga
     2701 ccgcgagcca gaacgggaag gcgatctccc ggaacgaccg gatcgacgac cggaacaggc
     2761 gctccgcttc ctcgtcgtcc gtgtcgggcg atcgcgcctc gatcctcgcg ccgtgcgcca
     2821 ggaagaaggt cgtcctccgg ccgatcggat ccgtccggac ggtcgtgagg atctcgtgga
     2881 cccttgtttg atccccgagc gccagcgccg cctcgagccc ggtcacgact ccctccttca
     2941 cgcacgagtg cgacaggccg agcgcctccc tcccttcgag ccctatctcg gccaaccgca
     3001 gggcctcctt cggatcgcct tccgccaagc ggagggtcgc cgcgccgcat gcgtactcgc
     3061 tgacctcctg gacgtccgca gaggtctgca ggtcggcgaa ccgctccaga cgtcgcagag
     3121 cggcagccag gtcgcctcgg tgcacctcca cggcggtccc gaacgacacg tagccctggt
     3181 tgaacgcgct ccgtgatcgt gcgaactcat ccggtgggat ctcggcgagc gatgccatga
     3241 ggtcgtccca ccgaccgagt gcgaacttcg ggtaggcctg gccgaggaac gtcgattccc
     3301 agaaccagtt tccgactcgg cgcgacagcg tgagcccctc atcgcccatc cggtcggctt
     3361 ccgcgtaccg gtcctcgtag tcgcacaggt cgacgaggtt gttgtaggcc cggagggcgg
     3421 ccgacggctt gtcgtgctcg agcgcgatcg tcagcgcatg ccgcatcagc cccatcgcct
     3481 cgctcgctcg gccgaaggtc tggaggatca gtgctttcga gttcagcccc tccgagagga
     3541 tctcggggag gtcgagggcc tccacgatct cgagggaccg ctcgatccag tccatcgctc
     3601 gctcggtctc gccgttgaag aacgtgaacc gaccgagctg ggcggcgagc tgcgcgaggt
     3661 cggcgtcggg ctcctcgtcg gccaggacgc gatacgacgc cgtcatcgac tcgagggcat
     3721 cgatcccttt gccctgcgac cagaccacct caccgagccg cgccgagacc cgggctgccg
     3781 ggtgcgtcgc gccctccgcc tcgaacagct cgatcgccgc gcggaagtgc gcggaggcgc
     3841 tgtcgacgtc gacgcgcacg ttcgccatca tgcccgcccg ttcatgaagc tccgcgaccg
     3901 cgagcgggtc gtccgtgagc tcgatcgcgc gctccgcgta gcgctgcgcc tcctcgctgg
     3961 cgccgagtga cgcggcccgc tcgctcgcct tcctcaccat ctccagggcc tcgacccgta
     4021 tccgctcggc gtcctcctcg tcgggcgccg cacgatacgc gtcgaggaga tgcgaggcga
     4081 cgacctcgac gatctcgtcc tcgtcgacgg ccgagcggag gtaggtggcc gccgcgaggt
     4141 gcctcgcctt gcgctcgtgc ttcgacatcg tgtcgtacgc gaccttcttc accaggtcct
     4201 ggaggaagcc gtactggcca cggtccgaca ggagcgggtc gatcgatatc gagaggacct
     4261 ccttgcggac gagcgagccc aggagggagg tgacctcctc atccgggagg cccgtgatcg
     4321 ccacgagccc ctggagcgtg aacgtccggc cgaggaccga cgcgtcctgg aggagcctcc
     4381 gttccgccgg atccagcccg tcgagccgtg cggcgatcag cgcctggagg gtctcgggca
     4441 cctcaagcgt ctcgaccgat ccctccagtc ggaagttccc gttctcccgg acgaggatcc
     4501 cgcggtcgat cagcatccgg acggtctcga cggcgtagaa ggggatcccc tcggcgcgtt
     4561 cgaggatgcg cttccggacg tcgtcgggaa gtcccgggat cggatcgctc agcagcgagc
     4621 ccatctcgtc gctgtcgagc ggctcgagga agatcgaggt gaagttgcgg gtgccggcgc
     4681 cccagctcgg ccggcgctcc gcgagctccg gtcgtgcgag cgtgagcacg aagatcggcc
     4741 ggtcgcgcga ccactccacc aggtactcga tgaagtcgag gagcgcgctg tcggcccaat
     4801 ggatgtcctc gaacaccatg atcgacgggc cccgatcggc gatccgctcg aagaagatcc
     4861 tgcaggcggc gaacaggttc tcctggtccc cgaccccgcc ctcctcgagc ccgagcaggt
     4921 gcccgaggcg tggcctcacg aaccggcgct cgtccgcgtc ggggatgtgc tcctcgatcg
     4981 cctgcgcgag cttggcggac gccgttgagg attcctcgtc ttcggcgatg cccgcgcgtc
     5041 ctcggatcat ctcggcgagc gcccagaagg cgacgccctc gccgtacgcg aggcaccgtc
     5101 cctggtgcca cc
//
LOCUS       DASUVE010000282         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_137317, whole genome shotgun sequence.
ACCESSION   DASUVE010000282 DASUVE010000000
VERSION     DASUVE010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137317"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VF036_06090"
     CDS             complement(<1..588)
                     /locus_tag="VF036_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6264832.1"
                     /translation="MSERTGQTRSDEEIMDLFRRLPDPSAREALATEFLPLAEYFARR
                     FSGRGEPVDDLTQTASLGLLNAIDRFDPERGVPFSTYAAATIVGELKRHFRDRGWALR
                     VPRNVQETAILVNRTVSTMWQDLGRAPTVAEIARAADIGEDDVLQALDALQAYTTDSL
                     DAPVGDASSTAAESIGSVDPSFQVSDDWLSIAPALR"
     gene            complement(585..980)
                     /locus_tag="VF036_06095"
     CDS             complement(585..980)
                     /locus_tag="VF036_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6264833.1"
                     /translation="MTNLPTAVSVDVPATAVFIQVLRNVVAGVAARLDLPIDQIEDIR
                     LAVTEAASLLLEEGDATSLQMSIGRDGDTMEVTLTSDGRSDPWPTDRVTASWPWLVVK
                     GLTDEVRADRAEGAGPSIGFTKRRERVDR"
     gene            982..1377
                     /locus_tag="VF036_06100"
     CDS             982..1377
                     /locus_tag="VF036_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6264834.1"
                     /translation="MSTYTTDAASLPRLQEVGTVDLTLDRRTHGRWAVVEVKGELDLY
                     TAPSFRESVLEAGDAEDPKVIVDFRGLGFIDSSGLGAIVACLKHIRERGGDLALVAPE
                     GSGLRRLMDLTGLDRVLTLYPSIDDISQD"
     gene            1392..1748
                     /locus_tag="VF036_06105"
     CDS             1392..1748
                     /locus_tag="VF036_06105"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6264835.1"
                     /translation="MSTSRLGVAGDPALALVVRIFVGSVTEQWGLSEPVREDLRLAAS
                     ELFSGAVEAGNRDLVRFELSTDEDGVELRAEDVGVEHESADLEGWTGRFALIRALFPD
                     ADVSDTTVRIRVPLTN"
     gene            1905..2444
                     /locus_tag="VF036_06110"
     CDS             1905..2444
                     /locus_tag="VF036_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264836.1"
                     /translation="MSAIQHHDADATSTETRAVSEAPSPWLALRVLLTVAAAVLLLVG
                     SFLEWVGGTDGSEIAVQAYWSTDAGTADFFSSAGLVTAICGAVIILGLAFRGGWLTRF
                     AGAVALVAFTLIVIQMGREGSLDTLQDVGMGLWFVLAGGILALIGGFFPSTRTVAAST
                     ASTDTDTDTDGSATHSHTD"
BASE COUNT          394 a          882 c          905 g          433 t
ORIGIN      
        1 gcggagcgcg ggggcgatgc tcagccagtc atcagagacc tggaagctcg ggtccacgct
       61 cccgatcgac tcggcggcgg tgctggacgc gtcgccgacc ggagcatcga gcgagtccgt
      121 cgtgtacgcc tgcagtgcat cgagggcttg caagacatcg tcttctccga tgtccgccgc
      181 ccgtgcgatc tccgcgacgg tcggagctcg gcccaggtcc tgccacatcg tcgacacggt
      241 gcgattgacg aggatcgccg tctcctgaac gttccgcggg acccggagcg cccacccgcg
      301 atcgcggaaa tgccgcttca gctcacccac gatcgtcgct gccgcgtagg tcgagaacgg
      361 cactccccgc tcgggatcga atcggtcgat cgcgttcaac aggccgagag aggcggtctg
      421 ggtgagatcg tcgacgggct cacccctgcc cgagaagcgt cgggcgaagt actcggcgag
      481 cggcaggaac tccgtggcga gcgcctcccg ggcggacggg tcgggcaatc gacggaagag
      541 atccatgatc tcctcgtcgg atcgtgtctg gccggtacgc tcgctcaccg atccacacgc
      601 tcacgacgct tggtgaaccc gatcgacgga ccggccccct cggcccggtc agctcggacc
      661 tcatcggtga gtcctttcac cacgagccag ggccacgacg ccgtgacgcg atcggtgggc
      721 cacgggtccg accgcccgtc ggacgtgagg gtgacctcca tggtgtcgcc gtcgcgaccg
      781 atcgacatct ggagcgacgt cgcgtcgccc tcctcgagca ggagcgacgc cgcctcggtc
      841 accgcgagcc ggatgtcctc gatctggtcg atcggcaggt cgagtcgcgc agccactccc
      901 gcaacgacgt tgcggagcac ctggatgaac accgcggtcg cagggacgtc cacggagact
      961 gccgtcggga ggttcgtcac ggtgtcgacc tatactaccg acgccgcatc gctccctaga
     1021 cttcaggagg ttggcaccgt ggacctgacc ctcgaccggc gcacgcatgg acgctgggcc
     1081 gtcgtcgagg tgaagggcga gctcgatctc tacacggccc ccagcttccg tgaatccgtt
     1141 ctggaggcgg gggacgccga ggatcccaag gtcatcgtgg acttccgcgg tctcggcttc
     1201 atcgactcgt ccggcttggg agcgatcgtc gcctgcctca agcacatccg ggaacgcggc
     1261 ggcgacctcg cgctcgtcgc gccggagggg tcgggtctcc gtcgtctgat ggatctcacc
     1321 gggctcgatc gcgtgctgac cctctatccg agcatcgacg acatctccca ggattgactg
     1381 ggccgacgag cgtgtccacc tcgcggttgg gtgtggctgg cgatccggcg ctggcgctcg
     1441 tggtgcggat cttcgtgggc tcggtgaccg agcagtgggg actctccgag cccgtgaggg
     1501 aggacctccg gctcgccgcc tctgagctct tctccggggc ggtcgaggcg gggaacaggg
     1561 acctcgtcag attcgaactc tcgaccgacg aagacggggt ggaactgcgg gcggaagacg
     1621 tcggggtgga gcacgagagc gccgacctcg agggctggac cggccgcttc gcgctgatcc
     1681 gggcgctgtt cccggacgcc gacgtgtctg acacgacggt ccggatccgc gtccccctca
     1741 cgaactaggc gttccgagcg acgttcacct cccggcaccc gagccacgga aggggttgaa
     1801 gccggtctcg gctgggtata tccggaccca gacgagggcg gccgatgcgg cccgtccaag
     1861 tgtggccagc gcggccggag cgcggccaga aggagggccg aaatatgtct gcgatccagc
     1921 atcacgacgc ggatgcgacc tcgacagaga cgcgagcggt cagcgaggcg ccttctccgt
     1981 ggctcgcgct ccgcgtgctc ctcaccgtcg ccgcggcggt gctcctgttg gtcggaagct
     2041 tcttggagtg ggtcgggggc accgacggat cggagatcgc cgtgcaggcc tactggtcca
     2101 cggatgcggg gacggccgac ttcttctcct cggcaggact ggtcaccgcg atctgcgggg
     2161 ccgtcatcat cctggggctc gcgttccgtg gtggctggct caccagattc gccggcgcgg
     2221 tggcactcgt ggccttcacc ctgatcgtga tccagatggg ccgcgaggga tcgctcgaca
     2281 ccctccagga cgtcgggatg ggcctgtggt tcgtgctggc gggcggcatc ctcgcgctga
     2341 tcggcgggtt cttcccgtcc acgcgcacgg tcgccgcgtc caccgcgtcc acggacacgg
     2401 atacggatac ggacggaagc gcgacgcact cgcacacgga ctagcggggc cgagcgtctc
     2461 cccgtggcgc atggatcgtg acccggccgt ctacggccgg gtcacgacga tcctggtgcc
     2521 gttgctctct ccgtccgcat cgtggttgct cgcggggagg tacgcgcgtg cctccttcga
     2581 gagccccaac accacgaact cgatgctcag acgg
//
LOCUS       DASUVE010000283         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_138091, whole genome shotgun sequence.
ACCESSION   DASUVE010000283 DASUVE010000000
VERSION     DASUVE010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138091"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VF036_06115"
     CDS             <1..949
                     /locus_tag="VF036_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6264837.1"
                     /translation="RLSATTGFLQSVWWQFLLVGVVAASVSLVVARLMARGMTQPLRD
                     MAAAARRMETGDYSARVHTRSRDEVGKLATAFDRMRGELELLEQSRRDLVANVSHELK
                     TPITAIRAHLENLLDGVEQPEPATLGVMLSQVERLGRLVDQLLDLSRLESGEVPLRIE
                     QLPLQPLVADLISEIDVALPGRGVDVHNDVPFDLPALSADRERVHQVLFNLVDNAVRY
                     TPDGGSVTVSARRRNGSVEVAVSDTGTGIPPEHLPRLFERFYRADPARGRGEGGTGIG
                     LAIARSVVEAHGGQIKAESRPGQGSIFTFDLPAADGSDR"
     gene            946..1395
                     /locus_tag="VF036_06120"
     CDS             946..1395
                     /locus_tag="VF036_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012629588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX6264838.1"
                     /translation="MKTVTTSLMVTPPERYAFLDLTDDLRRAVKDAGITEGLVVAFCA
                     HTTCALLINEWEDGALDDFRRHLTELVPHEGAYYRHDDFELRTQNLHADERKNGHAHV
                     KAMLLSATSHAIPVVDGEPTLGTWQRLLFFEMDEPKERTITLQVFGS"
     gene            1409..2494
                     /gene="ispG"
                     /locus_tag="VF036_06125"
                     /gene_synonym="gcpE"
     CDS             1409..2494
                     /gene="ispG"
                     /locus_tag="VF036_06125"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX6264839.1"
                     /translation="MIQRRSTRRLMVGTVAVGGDAPISVQSMTTTKTADINATLQQIA
                     SLVAAGVDIVRVAVPHWEDANALEAIAAKSTVPVIADIHFQWKYAIAALEAGIQGLRI
                     NPGNIKYQDKVRVIAREAKARGVPIRIGVNAGSLEKDLLARYGAPTPEALVESALGEA
                     RILEDEDFFDIKISVKHSNPMVMIEAYRQLAERCDYPLHLGVTEAGPMPMGGVKSAVG
                     IGTLLAEGIGDTIRVSLTDDPVEEVKVGTGILQSLGLRTRGLDLVACPSCGRAEVDVL
                     GLTKQVNEALEREGLKVPIRVAVMGCVVNGPGEAREADLGIAAGKGQGFLVVKGEVVD
                     KIPETDFVTRLVAEAKKIAAATSVGSD"
     gene            2532..>2742
                     /locus_tag="VF036_06130"
     CDS             2532..>2742
                     /locus_tag="VF036_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264840.1"
                     /translation="MKRILGRSIAGAALVLSAVPSMPAGAADVPGTTCSVFPSDNAWN
                     MDIRRLPRHPKSGTWKRATHAGSTRL"
BASE COUNT          446 a          928 c          961 g          407 t
ORIGIN      
        1 ccgtctctcg gcgacgaccg gcttcctgca gagcgtctgg tggcagttcc tcctcgtggg
       61 cgtcgtggcg gcgagcgtgt ccctcgtcgt ggcgcggctc atggcgcgag gcatgaccca
      121 gccgctgcgg gacatggctg cagcggcccg gcggatggag acgggcgact actccgcgcg
      181 cgtgcacaca cggagtcggg acgaggtcgg gaagctcgcc acggcattcg atcgtatgcg
      241 cggcgagctg gagctcctgg agcagagccg ccgagacctc gtggccaacg tgtcgcacga
      301 gctgaagacg ccgatcaccg cgatccgggc tcacctcgag aacctcctcg acggtgtgga
      361 gcagccggag ccggccacgc tcggcgtgat gctctcgcag gtcgagcggc tggggcgtct
      421 cgtcgatcag ctgctggatc tctcgcggct ggagtccggc gaggtcccgc tccggatcga
      481 gcagctgcct ctccagcccc tcgtcgcgga tctgatctcc gagatcgacg tcgcgctgcc
      541 gggacggggc gtcgatgtgc acaacgacgt tccgttcgat ctccccgcgc tctcggccga
      601 ccgcgagcgc gtgcatcagg tcctgttcaa cctggtcgac aacgcagttc gatacacgcc
      661 ggacggaggg agcgtcaccg tctccgcccg ccgccggaac ggctccgtcg aggtcgccgt
      721 ctcggatacg ggaaccggca tccctcccga acacctcccg cggctcttcg agcgcttcta
      781 ccgggccgac cccgcccggg gacgcgggga aggcggaacc gggatcgggc tcgcgatcgc
      841 gcgatccgtc gtcgaggcgc acggcgggca gatcaaggcg gagagccggc ccggacaggg
      901 cagcatcttc acgttcgacc tcccggccgc ggacgggagc gaccgttgaa gaccgtcacg
      961 acgagcctca tggtcacgcc gcccgagcgg tacgcgttcc tcgacctcac cgacgacctc
     1021 cgccgtgcgg tcaaggatgc gggcatcacg gagggtctcg tcgtcgcgtt ctgcgcgcac
     1081 acgacctgtg ccctgctcat caacgagtgg gaggacggcg cactcgacga cttccgtcgt
     1141 cacctgaccg agctagtccc gcacgagggc gcgtactacc ggcacgacga cttcgagctc
     1201 cggacccaga acctccacgc ggacgagcgc aagaacggcc atgcccacgt gaaggcgatg
     1261 ctcctgtcgg ccacctcgca tgcgatcccg gtcgtcgacg gcgagccgac gctcggcacg
     1321 tggcagcggc tgctcttctt cgagatggac gagccgaagg agcgcacgat caccctgcag
     1381 gtcttcggga gctgagagac tcgtgctgat gatccaacgt cgctcgacgc gccggctcat
     1441 ggtgggcacc gtcgccgtgg ggggcgacgc gccgatctcc gtccagtcga tgaccacgac
     1501 gaagaccgcg gacatcaacg cgacgctgca gcagatcgcc tcgctcgtcg ccgccggcgt
     1561 ggacatcgtc cgggtggcgg tacctcactg ggaggacgcg aacgccttgg aggcgatcgc
     1621 cgcgaagtcg accgtgccgg tgatcgccga catccacttc cagtggaagt acgcgatcgc
     1681 ggccctcgaa gcagggatcc aagggctccg catcaacccg gggaacatca agtaccagga
     1741 caaggtccgc gtgatcgcgc gagaggcgaa ggcccgcggt gtcccgatca ggatcggcgt
     1801 gaacgccgga tcgctcgaga aggacctgct ggctcgctac ggtgcgccca cgcccgaggc
     1861 cctcgtcgag tcggccctcg gggaagcgcg catcctcgag gacgaggact tcttcgacat
     1921 caagatctcg gtgaagcact cgaacccgat ggtgatgatc gaggcctatc ggcagctggc
     1981 ggagagatgc gactacccgc tgcacctcgg cgttaccgag gccggcccga tgccgatggg
     2041 gggtgtcaag agcgccgtcg ggatcgggac gctcctcgcc gaggggatcg gcgacacgat
     2101 ccgcgtctcg ctgaccgacg accctgtgga ggaggtcaag gtcgggacgg ggatcctgca
     2161 gtcgctgggc ctgcggacgc gcggcctcga tctcgtcgcc tgcccctcct gcggacgggc
     2221 ggaggtggat gtgctcggac tcacgaagca ggtgaacgag gcgttggagc gcgaggggct
     2281 gaaggtgccg atccgcgtcg cggtgatggg gtgcgtcgtg aacggccccg gcgaggcgcg
     2341 ggaggcggac ctcgggatcg ctgcgggcaa gggccaaggg ttcctcgtcg tcaagggtga
     2401 agtcgtcgac aagatccccg agaccgactt cgtcacgcgt ctggtggcgg aggcgaagaa
     2461 gatcgccgcc gccacatccg tgggctccga ctgagctcaa cgcagccgtc gcggcgaccg
     2521 atacccgtgt gatgaagcgc atcctcggac gatcgatcgc aggcgcggcg ctcgtcctct
     2581 ccgcggtgcc gtcgatgccg gcgggcgcgg cggacgtccc gggcaccacg tgctcggtct
     2641 tcccttcgga caacgcgtgg aacatggaca tccgcaggct gccgcgccat cctaagagcg
     2701 gcacctggaa gcgtgcgacg cacgccggtt ccacccggct gc
//
LOCUS       DASUVE010000284         3503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_891424, whole genome shotgun sequence.
ACCESSION   DASUVE010000284 DASUVE010000000
VERSION     DASUVE010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891424"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..451
                     /locus_tag="VF036_06135"
     CDS             101..451
                     /locus_tag="VF036_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HEX6264841.1"
                     /translation="MIVEKPWGKVVTYALNQPSSVRVITVEPGQETSVHYHRMRDETW
                     VVLDPGLSIEIGDRTVDAGPGEEFVIPAEQAHRIRCNGSGPGRILEVAYGYTTEDDTQ
                     RLQDAYGRSLEPDW"
     gene            582..1169
                     /gene="infC"
                     /locus_tag="VF036_06140"
     CDS             582..1169
                     /gene="infC"
                     /locus_tag="VF036_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007263145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX6264842.1"
                     /translation="MNDRIRSPQVRLVGADGEQIGVVATQEAVRRARELDLDLVEVAP
                     QANPPVCRIMDYGKFKYERDIRQKEARKKQARTELKEIKFRPKIDAHDYGTKKGHVER
                     FLRGGHKVKVTIMFRGREMTHTELGRKILDRLVGDLGEMVTVESMPKQEGRNMIMVIS
                     PNKRYMEQLARARARESAPVVDAEPVASAAETDEG"
     gene            1173..1370
                     /gene="rpmI"
                     /locus_tag="VF036_06145"
     CDS             1173..1370
                     /gene="rpmI"
                     /locus_tag="VF036_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX6264843.1"
                     /translation="MPKNKSHRATAKRFRITRNGKVLHRKATGNHMLTKKSSSRRRRV
                     EGMAEVGSEADAKTVKRLLGE"
     gene            1374..1739
                     /gene="rplT"
                     /locus_tag="VF036_06150"
     CDS             1374..1739
                     /gene="rplT"
                     /locus_tag="VF036_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX6264844.1"
                     /translation="MARIKRSVHAKKKRRDVLEAAKGYTGSRRKRRRVAKEQVMHSGM
                     YAYRDRRDRKAQFRRLWITRINAAVRPHGLSYSRFVSGLHAAGVEVDRKILADLAVRD
                     PGAFAQLVETAKASGPSDS"
     gene            1797..3233
                     /locus_tag="VF036_06155"
     CDS             1797..3233
                     /locus_tag="VF036_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX6264845.1"
                     /translation="MTSGRNPKVVAAVRLKKRALREADRRFLVEGAQAVAEALAEDGR
                     LESLFVEDDLDPLAIRAKQAGVLVDHVTDRVMERLTSTVSPQGVVGIAPFVDVTLEEL
                     GPPGAVALLHEVRDPGNAGTILRSADAAGAVGVVFAGSSVDAYNAKSVRASAGSIFHV
                     PVVRDVSTEGAIATLRERGFTIVAMDVHGDEDLFEAQLPPSPAFVFGNEAHGLPADIL
                     GTAERRVRVPQAGRAESLNLAAAATVCLFEWVRRSRATTGTLEALVAAAAHDIRSPLT
                     AMKGFGYALGRRWTDMTEAQRDLMLTGIVHDADRMDTIVRQLLDAARVMSGTFQSFAE
                     LVDVSDVVRQITEQQARDPEHPPIEWAGGTGTKAMLDGGRLKTTLLAFTEALVWWASD
                     GPIRIEGALRSGRLVVSASREAHGDVDVASLFEARRPGMGGGSKIGLYVARRVAEAQG
                     GSASGDLADGRLTLRVELPAAGPEPIAP"
     gene            3247..>3503
                     /gene="pheS"
                     /locus_tag="VF036_06160"
     CDS             3247..>3503
                     /gene="pheS"
                     /locus_tag="VF036_06160"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014909.4"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX6264846.1"
                     /translation="MDVQRMRESVEEVRERGRRAFAEASSREELEAAQVSVLGRKSRL
                     AEVQRSLGSLDPEERKDLGRIVNEVRAELEGAASRRREELES"
BASE COUNT          591 a         1143 c         1278 g          491 t
ORIGIN      
        1 acaaggggtg ggaggccgcg gaaaccgcgc tggagatggc cgacctgctc gaccgattgc
       61 ccggcgggac ccgacgggag caggaggaag ggagcactcg atgatcgtgg agaagccgtg
      121 ggggaaggtg gtcacgtacg ccctcaacca gccgtcgtcg gtccgggtca tcacggtgga
      181 gcccggtcag gagacgagcg tccactacca ccggatgcgc gatgagacgt gggtcgtcct
      241 cgacccgggc ctctcgatcg agatcggtga tcggaccgtc gacgccgggc cgggcgagga
      301 gttcgtgatc cccgcggagc aggcgcatcg catccgctgc aacggctccg gtccgggacg
      361 catcctcgaa gtggcgtacg gctacacgac cgaggacgac acgcagcgcc tccaggatgc
      421 gtacgggcgc tccctcgagc cggactggtg aggcccatcg acaggggtca gaggctgccc
      481 ctatactctg cgcacgtgtt ccgtctgatc cgccacaagc accggctggt ccggtgcttc
      541 gttttcgaag ggagtgatgc ggggtctcga ccgaaccacg cttgaacgac aggatccgat
      601 ctccccaggt gcgcctcgtc ggcgccgatg gcgagcagat cggggtggtc gcgacccagg
      661 aagccgtccg cagggcacgt gagctcgacc tggacctcgt cgaggttgca ccccaggcga
      721 acccgccagt ctgtcggatc atggattacg gcaagttcaa gtacgagcga gacatccgtc
      781 agaaggaagc tcggaagaag caggcccgga ccgagctcaa ggagatcaag ttccgcccca
      841 agatcgacgc gcacgactac ggcacgaaga agggccacgt cgaacggttc ctcaggggcg
      901 gccacaaggt gaaggtgacc atcatgttcc ggggtcgaga gatgacccac acggagctcg
      961 ggcgcaagat cctggaccgg ctggtgggag acctgggtga gatggtgacc gttgagtcga
     1021 tgccgaagca ggaagggcgg aacatgatca tggtgatctc gcccaacaag cggtacatgg
     1081 agcagctcgc ccgcgcacgg gctcgcgaat cggcacccgt cgtcgatgcc gagccggtcg
     1141 cctctgcagc ggagacggac gagggatagc cgatgccgaa gaacaagagc caccgagcca
     1201 ccgcgaagcg cttccggatc acccggaacg gcaaggtgct ccaccgcaag gccacgggca
     1261 accacatgct cacgaagaag tcgtcgtcgc gccgccgccg cgtcgagggc atggccgagg
     1321 tcggctcgga agccgacgcc aagaccgtca agcggctgct gggggaatga tcgatggcgc
     1381 gcatcaagcg ttcggtgcac gcgaagaaga agcgccgcga tgtcctcgag gccgcgaagg
     1441 gctacacggg gagccggcgc aagcggcgcc gggtggcgaa ggaacaggtc atgcactccg
     1501 gcatgtacgc ctaccgcgac cgccgggacc gcaaggcaca gttccgtcgc ctctggatca
     1561 cgcgcatcaa cgcggcggtc cgccctcacg ggctgtcgta ctcccgcttc gtgagcgggc
     1621 tgcacgccgc cggcgtcgag gtcgaccgca agatcctcgc cgacctggcg gtccgtgacc
     1681 cgggggcctt cgcgcagctc gtcgagaccg cgaaggcgtc gggtccctcc gactcctagc
     1741 gggccgttgg ggcccgcgta cactccgccc gtgcgagggg tgcgcgagtc gccgtgttga
     1801 cgtccggcag gaacccgaag gtcgtcgctg ccgttcggtt gaagaagcga gccctccgcg
     1861 aggcggatcg tcgattcctc gtcgagggcg cgcaggcggt ggccgaggcg ctggccgaag
     1921 acggacgact cgagtcgctg ttcgtggagg acgatctcga cccgctcgcg atccgtgcga
     1981 agcaggccgg cgtgctcgtc gaccacgtca cggaccgggt catggaacgg ctgacgtcca
     2041 ccgtctctcc acagggcgtc gtggggatcg ccccgttcgt cgacgtgacc ctcgaggagc
     2101 tgggcccacc gggcgcggtc gcactcttgc acgaggtccg ggatcccggc aacgcgggca
     2161 cgatcctccg ctcggccgac gctgcgggcg cggtcggggt cgtgttcgcc ggaagctcgg
     2221 tggacgcgta caacgccaag agcgtccgcg cgtcggccgg ctcgatcttc cacgtcccgg
     2281 tcgtgcggga cgtctcgacg gagggcgcga tcgcgaccct ccgggagcga gggttcacca
     2341 tcgtcgccat ggatgtgcac ggcgacgagg acctgttcga agcgcagctc ccgccctcgc
     2401 ccgcgttcgt cttcggcaac gaggcgcacg gtctccccgc cgacatcctc gggacggccg
     2461 agcgacgcgt gcgcgtgccc caggcgggcc gcgcggagag cctcaacctc gcggctgccg
     2521 ccacggtctg tctgttcgaa tgggtccggc gaagccgtgc gaccaccgga accctcgagg
     2581 cgctggtcgc ggccgccgcg cacgacatcc ggtcgccgct gacagccatg aaggggttcg
     2641 gctacgcgct cggcaggcgg tggacggaca tgacggaagc gcagcgcgac ctgatgctca
     2701 cgggcatcgt ccacgacgca gaccgcatgg acacgatcgt ccggcagctc ctcgacgccg
     2761 ccagggtgat gagcgggacc ttccagtcgt tcgccgagct cgtggacgtg tccgacgtgg
     2821 tgcgtcagat caccgagcag caggctcgtg acccggaaca ccccccgatc gagtgggccg
     2881 gagggaccgg gaccaaggcg atgctcgatg gcggccggct gaagacgacg ctcctcgcgt
     2941 tcacggaggc gctggtgtgg tgggcgagcg acgggccgat ccggatcgag ggcgcgctgc
     3001 ggagcgggcg cttggtcgtg tccgcttcac gggaggccca cggcgacgtg gatgtggcct
     3061 ctctgttcga agcccggcgc cccgggatgg gcggcggctc gaagatcggc ttgtacgtag
     3121 cgcgacgcgt ggccgaggcc cagggaggga gtgcctcggg cgacctcgcc gatggacggc
     3181 tgacgctccg cgtggagctc cccgccgccg gccccgaacc tatcgcgccg tagactcggc
     3241 gacctcatgg acgtccagcg gatgcgcgag tcggtcgagg aagtgcgcga gcgcggccgc
     3301 cgtgcgttcg cggaggcctc ttcgcgcgaa gagctggagg ctgcgcaggt ctcggtcctc
     3361 ggacggaaga gccgtctcgc cgaggtgcag cggtccctcg ggtccctcga tccggaggag
     3421 cgcaaggatc tgggtcggat cgtgaacgag gtccgagcgg agctcgaggg tgcggcctcg
     3481 aggcgacgtg aggagctcga gtc
//
LOCUS       DASUVE010000285         3331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_140687, whole genome shotgun sequence.
ACCESSION   DASUVE010000285 DASUVE010000000
VERSION     DASUVE010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140687"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VF036_06165"
     CDS             <1..209
                     /locus_tag="VF036_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX6264847.1"
                     /translation="EISARNQLKGTVRSVELGTVMAEVVVDIGGQEIVAAITRGSVEH
                     LALAAGAEVTVIIKATEVMIATGS"
     gene            276..932
                     /gene="lepB"
                     /locus_tag="VF036_06170"
     CDS             276..932
                     /gene="lepB"
                     /locus_tag="VF036_06170"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX6264848.1"
                     /translation="MRTDERSVRKAARQGDSPKPKKRRHPALTFLSELPGLILMAFAL
                     ALLIKSTLLQAFWIPTGSMEPTLVPGDRVIVAKVPYYFHDPQRGDVIVFEEPDPAKEP
                     ERGVWGAITHWLGQGLGFTPPDDPDYIKRVIGEPGDVVSARDGDVYVNDIRISEPYLH
                     ERTARFPETTVPEGELFVMGDNRSNSLDSRFSLGFVPIDRVVGKAVWIIWPVENMSGF
                     "
     gene            946..1335
                     /locus_tag="VF036_06175"
     CDS             946..1335
                     /locus_tag="VF036_06175"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEX6264849.1"
                     /translation="MRELLIAGGILVGLWLLAAVVLLAIGRKTTSRELVTFLPNLIRL
                     FRGLLRDERVPRSSKVLLLLGGFWLASPIDLIPEFLPGVGALDDAVVAGLVLRHLVKR
                     AGPDVVKDHWRGDPKTIGLMLRAARVS"
     gene            complement(1407..2777)
                     /locus_tag="VF036_06180"
     CDS             complement(1407..2777)
                     /locus_tag="VF036_06180"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX6264850.1"
                     /translation="MTTLEIDPVAKELGDRFQAAGHELYLVGGVVRDLVMGRLAPGVD
                     TDFATDASPAETSRLLRGWADALYLVGAKFGTVGARRHGNVLEITTFREERYPEDERK
                     PAVTFAKDIQTDLSRRDFTINAMAVRLPDGEFVDPYEGVKDLAAKVLDTPLDPEIAFS
                     DDPLRMLRAARFVSQLGVTPAPRVVDAIHRMRDRLRIVSSERIRDELDKLLIGDAAGA
                     GLTILVDSGLADEFRPELPALQLEQDPVHRHKDVLRHTFAVVERLEPDPVLRLAGLLH
                     DIGKPSTRAITPDGVSFHHHEVVGARMAEQRLRELRFPNSTVEEVRTLVELHLRFHGY
                     GDGWTDAAVRRYVRDAGPLLDRLNQLTRADCTTRDPKRAERFARLQDELEDRIARLAE
                     QENLDAMRPTLDGRQVMERLALEPGPVVGEALAFLMDVRMERGEIPEDEAYELLDAWA
                     KEREIG"
     gene            2831..3196
                     /locus_tag="VF036_06185"
     CDS             2831..3196
                     /locus_tag="VF036_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264851.1"
                     /translation="MPIWLGFVWTLPNTLLGLVLGAFTFQLPRLDHGVVIFDRAARGL
                     TALLPRLGRTAMTVGFVIVSARPLEGRLLAHERHHVRQYLAWGPFFIPAYLVVAVRYG
                     YRRHPMELAAVRAAEAADP"
BASE COUNT          485 a         1136 c         1147 g          563 t
ORIGIN      
        1 tggagatcag cgctcgcaat cagctcaagg ggacggtgag atccgtggaa ctcgggaccg
       61 tcatggccga ggtggtggtc gacatcggcg gtcaggagat cgtggcggcg atcacgcgag
      121 gaagtgtgga gcacctggcg ctcgcggcag gcgcagaggt gaccgtgatc atcaaggcga
      181 ccgaggtgat gatcgcaacc ggctcgtgag cacgtccggc gcacggatcc tcctgatgcg
      241 gcccacccgg aacggaggac cggtaccctg agctggtgag gacggacgag cgttcggtcc
      301 gcaaggcggc ccgacagggc gacagcccca agcccaagaa acgtcgccac ccggcgctca
      361 cgttcctctc ggagctgccg ggtctgatcc tcatggcgtt cgccttggcg ttgctcatca
      421 agagcaccct gctccaggcg ttctggatcc ccacgggctc gatggaaccc acgttggtcc
      481 cgggcgaccg ggtcatcgtc gccaaggtcc cctattactt ccacgacccg cagcgtggcg
      541 acgtcatcgt gttcgaagag cccgaccccg ccaaggaacc ggagcgcggc gtgtggggtg
      601 cgatcaccca ctggctcggg cagggcctcg gtttcacccc gcccgatgac cccgactaca
      661 tcaagcgggt gatcggtgaa ccgggtgacg tggtgtccgc gagggacggc gacgtctacg
      721 tgaacgacat ccggatctcg gagccgtatc tccacgaacg caccgcccgg ttcccagaga
      781 cgacggtccc cgagggggag ctgttcgtga tgggcgacaa ccgctccaat tcgctcgact
      841 cccggttcag cctcgggttc gtcccgatcg accgggtggt gggcaaggcg gtctggatca
      901 tctggcccgt cgagaacatg agcggcttct agcgagggcg acctcgtgcg cgagctcctg
      961 atcgcgggtg gcatcctggt gggcctctgg ctgctcgccg ccgtggtgct cctggcgatc
     1021 gggcggaaga cgacctcacg ggagctcgtg accttccttc cgaacctcat ccgtctgttc
     1081 cgcggtctgc tccgtgacga acgtgtgccc cgttcttcga aggtcctcct gctgctcggc
     1141 ggcttctggc tcgcttcccc gatcgacctg atccccgagt tccttccggg tgtgggcgcc
     1201 ctcgacgacg ccgtcgtcgc ggggctcgtc ctgcgacacc tcgtgaaacg ggccggcccc
     1261 gacgtcgtga aggaccactg gcggggagac ccgaagacca tcgggctcat gcttcgggct
     1321 gctcgcgtct cctagtcgta ccagacgcct accgtcgaac gggacaacga ggagttcctc
     1381 aaccagcttc ggtccggttc cgctcgctag ccgatctccc gctccttcgc ccaggcgtcc
     1441 agcagctcgt acgcctcgtc ctcggggatc tcgccccgct ccatgcgcac gtccatcagg
     1501 aacgcgagcg cctcgcctac cacgggcccg ggctccaagg cgagtcgctc catcacctgc
     1561 cggccgtcga gggtcgggcg catcgcgtcg aggttctcct gctcggccag gcgggcgatg
     1621 cggtcctcga gctcgtcctg gagccgggcg aaccgctcgg cgcgcttcgg gtcgcgtgtc
     1681 gtgcagtccg ctcgcgtgag ctgattcaac ctgtccagga gcggaccagc gtcgcggacg
     1741 taccggcgga ccgcggcatc cgtccagccg tcgccgtacc cgtggaagcg gaggtgcagc
     1801 tcgacgagcg tccgcacctc ctccacggtg gagttcggga agcggagctc gcggagccgc
     1861 tgctccgcca tccgtgcgcc gacgacctcg tggtggtgga acgacacgcc gtcgggcgtg
     1921 atcgcccgag tcgagggctt cccgatgtcg tggaggagac cggcgaggcg gagcacggga
     1981 tcgggctcga gccgttcgac gaccgcgaac gtgtggcgca gcacgtcctt gtgccggtgc
     2041 acgggatcct gttcgagctg gagagccggg agctcgggac ggaactcgtc cgcgagcccg
     2101 gagtcgacga gtatcgtcaa tccggcgccg gccgcatcgc cgatcaggag cttgtcgagc
     2161 tcgtcgcgga tgcgctccga ggacacgatc cggaggcgat ctcgcatccg gtggatcgcg
     2221 tccaccacgc gcggagccgg tgtcaccccg agctgggaga cgaaccgcgc ggcgcggagc
     2281 atgcgcaggg gatcgtccga gaaggcgatc tccggatcca gaggcgtgtc gagcaccttc
     2341 gcggcgaggt ccttcacgcc ttcgtagggg tcgacgaact ccccgtccgg cagccggacg
     2401 gccatcgcat tgatcgtgaa gtcgcggcgg gacaggtcgg tctggatgtc cttcgcgaac
     2461 gtgaccgcgg gcttccgttc gtcctccgga tagcgctcct ctcggaacgt cgtgatctcc
     2521 aggacgttgc cgtggcggcg cgccccgacg gtgccgaact tggctccgac caggtacagc
     2581 gcgtccgccc atccgcggag caaccgggag gtctcggccg gcgatgcgtc cgtcgcgaag
     2641 tcggtatcga caccgggtgc gagccggccc atcaccaggt cgcgaacgac tcccccaacg
     2701 aggtagagct cgtgaccggc ggcctggaag cggtcgccga gctccttcgc gacgggatcg
     2761 atctcgagcg tggtcacgtc gtcaacctta ccgtcggggc tcagccgcac cggcgcgata
     2821 cggtgccgcc gtgccgatct ggctcgggtt cgtctggacg ctgccgaaca cgctgctcgg
     2881 gctcgtgctc ggtgccttca cgttccagtt gccgcggctc gatcacggcg tggtcatctt
     2941 cgatcgtgct gcacgggggc tcacggcgtt gctgccacgt ctcggtcgta ccgcgatgac
     3001 cgtcggcttc gtgatcgtgt cggcccgccc gctcgagggg cgactcctgg cccacgagcg
     3061 ccatcacgtg cgtcagtacc tcgcgtgggg cccgttcttc atccccgcgt acctcgtggt
     3121 cgcggtccgc tacgggtacc ggcgtcatcc gatggagctc gccgccgttc gcgccgcgga
     3181 agcggcggat ccgtagacga tcagtacggg aggctctcgg cggccgcgtc cgcctcatcg
     3241 aacgccgcgc ggcgttcgag cgtaccgtgc agcgccaggt cgaagtcgtg ggggttggtc
     3301 gcggtccggc gggcctcgtc cggcgagacg a
//
LOCUS       DASUVE010000286         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_708179, whole genome shotgun sequence.
ACCESSION   DASUVE010000286 DASUVE010000000
VERSION     DASUVE010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708179"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VF036_06190"
     CDS             complement(<1..368)
                     /locus_tag="VF036_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX6264852.1"
                     /translation="MSEEHEAEGTAKFTLRGTVIGLITPAIATAIAFPLAPTRPEISI
                     ALFMLAVVIAAVAGGLGAGLIAGILASTIRPLVVEDPQLTFRFDDVEDVATGVVFLSV
                     ALIVGLLVGQASEDRARASRR"
     gene            711..1367
                     /locus_tag="VF036_06195"
     CDS             711..1367
                     /locus_tag="VF036_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX6264853.1"
                     /translation="MHVIIMGCGRVGSELANAIADEHDVAIIDKNPEAFHAYPPGSAR
                     QIVGLGFDRDVLENAGIKDADAFVAVSSGDNSNIVSARVALEYYHVPKVIARIYDSRR
                     AEIYERLNIPTVATTRWGVKQIQLMLFHDREEIRETLGGGDLLRLRVPVADHLAGKPV
                     SSLEVDGKVRVAGISRGGGGFIPTASSTLQAGDYVILMISKDGLELVDGLTAPEDHQA
                     "
     gene            1364..2059
                     /locus_tag="VF036_06200"
     CDS             1364..2059
                     /locus_tag="VF036_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX6264854.1"
                     /translation="MKVVVTGGGAVGRHLAQDLTDRGHDVTLVEQRRELAEKLGQWAP
                     DTRIVYGDACEPRILENADVRSADVVVAATGDDEDNLVTSLLAKQEFGVPRVLARVNH
                     PKNEWLFDEQWGVDASVSPPHILTAMVEEAVTVGDLVRLLRLEGGKVALVEMRLPDQS
                     PNVGRALYELRLPTDTTLVAILREGHVMIPQPETALAAADEIVALSSVDSEAALRDTV
                     MGTGAGAPGAPDA"
     gene            complement(2067..>2363)
                     /locus_tag="VF036_06205"
     CDS             complement(2067..>2363)
                     /locus_tag="VF036_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX6264855.1"
                     /translation="ETRAWAETIEGSVRIAGAPVRQPVRIAGVIRRMTVLPMEGKEAL
                     QALISDGTGEVMIVFMGRRGIGGLSLGKRVVVDGVLADQRGEIRMVNPRLEFAG"
BASE COUNT          380 a          843 c          793 g          347 t
ORIGIN      
        1 cggcgggagg cgcgcgcccg gtcctcgctc gcctgaccga ccaacaggcc cacgatgagc
       61 gccaccgaga ggaagacgac ccccgtcgcg acgtcttcca cgtcgtcgaa ccggaacgtg
      121 agctgcgggt cctcgacgac gagcggcctg atcgtcgagg cgaggatccc ggcgatgagg
      181 ccggccccca acccgcccgc cacggcggcg atcacgaccg cgagcatgaa cagggcgatc
      241 gagatctcgg gtcgggtggg agccaagggg aacgcgatcg cggtggcgat cgcgggggtg
      301 atcagcccga taaccgtccc ccgcagcgtg aacttcgcgg ttccctcggc ctcgtgctcc
      361 tcgctcacga ccacgacacg ctagcccctt tcacgggatc ttcacgcctc gggaaggatc
      421 cttcacgctt ccctcacgcc cttgtctcga agatgagcgc agaggaaccc atcctccgag
      481 tcaaatccgg gggccgacag ctccctccag ttgttggact ccggatccgt cctcatcatt
      541 cgggcccgct ctgcgggccc gaactctttc ccgagcgtcc acgatccgac ctcagcgtcc
      601 acgatccgac ttcgtccccc ggcagagggg gaacaccacc gcggaaccga cggcctccca
      661 accgcctcct cttggtgtgc ggcacacgca cgggtaggct tccccaccgt atgcacgtga
      721 tcatcatggg ctgtgggcgg gtcggctccg agctggccaa cgcgatcgcc gatgaacacg
      781 acgtcgcgat catcgacaag aaccccgagg cgttccacgc ctatcccccc gggagcgcgc
      841 gccagatcgt ggggttgggc ttcgatcggg acgttctgga gaacgccggt atcaaggacg
      901 cggatgcgtt cgtggcggtc tcgagcggcg acaactcgaa catcgtctcg gcccgcgtgg
      961 cgctcgagta ctaccacgtg cccaaggtca tcgcccggat ctacgactcg cgtcgagcgg
     1021 agatctacga gcgtctgaac atcccgacag tcgccaccac gcgatggggg gtcaagcaga
     1081 tccagctcat gctgttccac gaccgcgagg agatccgcga gacgctcggc gggggtgacc
     1141 tcctgcggct ccgggtcccg gtcgccgacc atctggccgg caagccggtg tcgtcgctgg
     1201 aggtcgacgg caaggtacgc gtggcgggga tctctcgcgg aggcggtggg ttcatcccca
     1261 cggcgtcctc cacgctccag gcgggtgact acgtgatcct catgatctcc aaggacgggc
     1321 tggagctggt cgacggactg acggcgcccg aggaccatca ggcatgaagg tcgtcgtgac
     1381 cgggggcggc gcggtcggcc ggcatctcgc gcaggatctc accgatcgcg gccacgacgt
     1441 cacgctggtc gagcagcggc gggagctcgc ggagaagctg ggccagtggg ctccggacac
     1501 ccggatcgtc tacggcgacg cctgcgagcc gcgcatcctc gagaacgccg acgtgcggtc
     1561 ggccgacgtg gtggtcgccg ccaccggcga cgacgaggac aatctcgtga cgtcgttgct
     1621 ggcgaagcag gagttcgggg tcccgcgcgt gctcgcacgg gtcaaccacc cgaagaacga
     1681 gtggctcttc gacgagcaat ggggcgtgga cgcgtcggtg tccccgcccc acatcctgac
     1741 ggcgatggtc gaggaggccg tcaccgtcgg ggacctcgtc cgcctcctcc gcctggaggg
     1801 cgggaaggtc gcgctcgtcg agatgcgcct gcccgaccag tcgccgaacg tcggccgggc
     1861 cctctacgag ctccgcctgc ccaccgacac cacgctcgtc gcgatcctcc gggagggaca
     1921 cgtgatgatc cctcagcccg agacggcgct cgcggcagca gacgagatcg tcgccctgtc
     1981 gagcgtcgac tcggaggcgg cgctccgcga caccgtcatg gggacgggcg ccggcgcgcc
     2041 cggcgccccg gacgcgtgag acccggctag ccggcgaact ccagacgagg gttgaccatc
     2101 cggatctctc cccgctggtc ggcgaggacc ccgtcgacca ccacgcgctt gcccagggag
     2161 aggccgccga tcccgcggcg gcccatgaac acgatcatca cctcgcctgt cccgtccgag
     2221 atcagggcct gcagcgcctc cttgccctcc atggggagga cggtcatccg gcggatcacg
     2281 ccggcgatcc gcacgggttg ccggaccggt gccccggcga tccggacgga gccttcgatc
     2341 gtctcggccc aggctcgggt ctc
//
LOCUS       DASUVE010000287         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_708693, whole genome shotgun sequence.
ACCESSION   DASUVE010000287 DASUVE010000000
VERSION     DASUVE010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708693"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1978
                     /locus_tag="VF036_06210"
     CDS             <1..1978
                     /locus_tag="VF036_06210"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEX6264856.1"
                     /translation="DVDYDPLDAVTTPQQALEEDAPLIRTDKEGQENNHVYSWETGNK
                     EATDIAFSQADVVVSLDTFYPRIHPAPLETCGCVADINPATGQATIYMTTQAPHLIRT
                     VFALVAGLPEDKIRIVSPDIGGGFGNKVPVYPGYVVATAASLLLGHPVKWIEDRSENL
                     ISTGFARDYHMRGELALKTDGTMLGLRASLLADEGAFYADAQPSKFKAGLFHIVSGSY
                     DLPAAHVSAQGAYTNKAPGGVAYRCSFRVTEASYLIERLVQNAAYQLGMDTVDLRKKN
                     FIEKEQFPYSSATGFVYDSGDYIGAMDEALEMIGYDQLRKEQAEARADGKLHGIGIAS
                     FTEVVGAGPAKDYDIIGVKMNDGAELRIHPTGKAILKISCQTQGQGHETTFAQIVAGE
                     LGIQPEDIKVMHGDTDNTPYGLGTYASRSTPTAGAATAMVARRVREKAMRLAAHLLEV
                     AEEDVEYEAGTFSVKGAPDQVKTIQEVAFAAYTNYPEDMEAGLEGVHYYNPPNLTFPF
                     GTYAVSVEVDPDTGVWKVLRMVAVDDCGVRINPMIVEGQITGGLTEGYATAAMQLITF
                     DEVGNAIGTNFMDYLIPTAWETPKFELGETVTPSPHHPIGAKGVGESATVGSPAAYVN
                     AVIDALQPLGVKNIDMPLTSAKVWEAIQAAKTDA"
     gene            2030..>2136
                     /locus_tag="VF036_06215"
     CDS             2030..>2136
                     /locus_tag="VF036_06215"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX6264857.1"
                     /translation="MSARGEPFVLATVVWRRSPSSGKAGATALITSDGRV"
BASE COUNT          414 a          709 c          710 g          303 t
ORIGIN      
        1 cgacgtcgac tacgacccgc tcgacgcggt gacgaccccg cagcaagcgc tcgaggagga
       61 cgccccgctg atccgaaccg acaaagaggg ccaagagaac aaccatgtct actcgtggga
      121 gaccggcaac aaggaggcga cggacatcgc gttctcacag gccgacgtcg tcgtctcgct
      181 cgacacgttc tatccgcgta tccatccggc tccactcgag acgtgcgggt gcgtcgcgga
      241 catcaacccc gcgaccggcc aggcgacgat ctacatgacc acacaagcgc cgcacctcat
      301 ccggacggtc ttcgcgctgg tagccggcct gcccgaagac aagatccgga tcgtgtcacc
      361 agacatcggg ggtggcttcg gcaacaaggt tccggtgtac ccgggctacg tcgtcgccac
      421 ggcggcctcg ctcctcctcg ggcatcccgt gaaatggatc gaggaccgct ccgagaacct
      481 catctcgacc gggttcgccc gcgactacca catgcgtgga gagctcgcgt tgaagaccga
      541 cggcacgatg ctcgggctgc gagcgtcgct gctcgcggac gagggtgcgt tctacgccga
      601 cgcccagccg tcgaagttca aagccggcct attccacatc gtcagcggct cgtacgacct
      661 cccggcagcc cacgtgtcgg cgcaaggggc gtacacgaac aaggcgccag ggggcgtcgc
      721 gtaccgatgc tccttccgcg tgaccgaggc gtcgtatctg atcgagcgat tggtgcagaa
      781 cgccgcctac cagctcggga tggacaccgt cgatctgcgg aagaagaact tcatcgagaa
      841 ggaacagttc ccgtactcct ccgcgaccgg gttcgtctat gactcaggcg actacatcgg
      901 cgccatggac gaggcgctgg agatgatcgg atacgaccag ctccggaagg aacaggccga
      961 agcccgcgcg gacggcaagc tccatgggat cggcatcgcg tcgttcaccg aggtcgtggg
     1021 cgccggaccg gcgaaggact acgacatcat cggcgtgaag atgaacgacg gcgccgaact
     1081 ccggatccat ccgaccggga aggcgatcct gaagatcagc tgccagacgc aggggcaggg
     1141 tcacgagacg acgttcgccc agatcgtggc cggcgagctg gggatccagc ccgaggacat
     1201 caaggtgatg cacggcgaca cggacaacac gccgtacggg ctcggcacgt acgcctcccg
     1261 gtccacaccc acggccggcg cggcaacggc gatggtcgct cgccgcgttc gggagaaggc
     1321 gatgaggctc gcggcacacc tcctcgaagt cgctgaggag gacgtcgagt acgaagccgg
     1381 cacgttctcc gtcaagggag cgcccgatca ggtcaagacg atccaggaag tggcgttcgc
     1441 cgcgtacacg aactacccgg aggacatgga ggccgggctg gaaggcgtcc actactacaa
     1501 cccgccgaac ctcacgttcc cgttcgggac ctacgcggtc tccgtcgagg tcgaccccga
     1561 taccggcgtg tggaaggtgc tccggatggt ggccgtcgat gactgcggcg tccggatcaa
     1621 cccgatgatc gtcgaggggc agatcaccgg cggcctcacg gagggctacg ccaccgccgc
     1681 gatgcagctg atcacgttcg acgaggtcgg caatgcgatc gggacgaact tcatggacta
     1741 cctgatcccg acggcgtggg aaacgccgaa gttcgagctc ggggagaccg tgacgccctc
     1801 gccgcatcac ccgatcggcg ccaagggcgt cggcgagtcg gcgacggtcg gctcccccgc
     1861 cgcctacgtg aacgcggtga tcgacgcgct ccagcccctc ggcgtgaaga acatcgacat
     1921 gccgctcacg tccgcgaagg tctgggaggc gatccaggca gcgaagacgg atgcgtaatc
     1981 cgggcgagga ccgggcgtga agcccgacat cctcgccgag gccgccgcga tgtccgctcg
     2041 aggcgagccg ttcgtgctgg ccacggtggt ctggcggcgc tcgccctcct ccgggaaggc
     2101 gggagcgacg gcgctcatca cgagcgacgg ccgggt
//
LOCUS       DASUVE010000288         3896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_142548, whole genome shotgun sequence.
ACCESSION   DASUVE010000288 DASUVE010000000
VERSION     DASUVE010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142548"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VF036_06220"
     CDS             complement(<1..444)
                     /locus_tag="VF036_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX6264858.1"
                     /translation="MAVVEDGFHVRANLIARQEHLHERFGGVVPEVAARAHVESLNPL
                     LEQALSEAGVGFKSLDGVAVTVGPGLVGALLVGMAAAKAISLSTGAPLIGVNHLEGHV
                     WANFLAHGEPDPPYVSLVVSGGHTMLVHIAEMHRHEVLGQTLDDAA"
     gene            complement(483..977)
                     /gene="rimI"
                     /locus_tag="VF036_06225"
     CDS             complement(483..977)
                     /gene="rimI"
                     /locus_tag="VF036_06225"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX6264859.1"
                     /translation="MRRRHLRGVMAIERQVYARPWSPNLFAAEITDPNGRCYLVARVH
                     KEIVGYGGLICYGDEAHITNVAVDPMHQGHGIATRLLAELIPGAIQMGARAVSLEVRV
                     TNWGAQRIYARFGFRPVGIRRNYYQEVSEDALIMWTDDVRSESYRRRLDEIEETLPQG
                     VRPG"
     gene            complement(988..1695)
                     /gene="tsaB"
                     /locus_tag="VF036_06230"
     CDS             complement(988..1695)
                     /gene="tsaB"
                     /locus_tag="VF036_06230"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX6264860.1"
                     /translation="MIVVGIETSTPHTSVAIGSERELLGAVSVTGKARQEAVTPALEQ
                     LCGWTGVELGQVGGIAVGIGPGLFTGLRVGIETAKTLAQVLSVPIVGITSLDALAFSV
                     RHTHKLIVAVIDGRRREVFSAIYRALPGGVVRGTEYTVGTPDHLCAELETVPGEMLAV
                     GDGAILYRNQLEGLGSRVEFASTIRAHPEAAALVELAVPRFLREEHDRLHDVVPMYLR
                     KSDAEIAWDNRDRRTGA"
     gene            complement(1692..2213)
                     /gene="tsaE"
                     /locus_tag="VF036_06235"
     CDS             complement(1692..2213)
                     /gene="tsaE"
                     /locus_tag="VF036_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX6264861.1"
                     /translation="MRLELATATADGTRDVGAALATLLLPRDTIVLTGDLGAGKTTLV
                     QGIGRGLGVEDHVASPTFTLVKEYAGSLDVAHVDVYRLERVQDVVDLGLDELGGRDRV
                     LLVEWGDVVQELLPEDRLRVQLTTGTDDDARRIVLTPHGRSWALRWERLEQLLQPFRR
                     DEPSDDPVAEAAP"
     gene            complement(2216..2914)
                     /locus_tag="VF036_06240"
     CDS             complement(2216..2914)
                     /locus_tag="VF036_06240"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX6264862.1"
                     /translation="MSRTLADIGAEAAGCTRCRLAGGRTQVVFGVGNPDADVLFIGEA
                     PGFHEDKQGEPFVGAAGHLLTKVLGDVLGLGRGDVYICNILKCRPPGNRDPQEDEIES
                     CTPWLVEQISSIQPRVIVTLGNFATKFVLQTQQGITRMRGRAYPWHGRTVIPTFHPAA
                     ILHGGGEKSRQFGELREDFELVRDTLAAMDPAPGSAPAPEPTPAPVQVPEPAPVRVPD
                     AEIAPAPEEQLELF"
     gene            complement(2911..>3896)
                     /locus_tag="VF036_06245"
     CDS             complement(2911..>3896)
                     /locus_tag="VF036_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6264863.1"
                     /translation="ALWRNRRAAHGDRAWGPPPEDLGPIEAFDGTALAVRAAGPPDGP
                     IMLFSHGFSLDMTTWREQWSDLGDEFRCIALDHRAHGRSEVPGTGDVSLRSMGRDLAT
                     VLDAVAPDRRVVVIGHSMGAMAILALAEQRPDLFGPRIGGVAFLGTSASDLLRGAMGG
                     VTHLLRPRLGSVATAARRVDRLRRAILAGPAEVPAIVARVTQFAPEAPDDVVEHVLHL
                     AEHTTSLVWTDGLSELMRMDFRHALARITVPALVVVGDQDRVTPPAVAVELAAALPNG
                     RLFLVEGAGHIAMLERPERLNPELRSFAREALSVGVGRGRMREGTRKSGAA"
BASE COUNT          570 a         1441 c         1314 g          571 t
ORIGIN      
        1 ggccgcgtca tcgagggtct gcccgagcac ctcgtggcgg tgcatctccg cgatgtgcac
       61 gagcatcgtg tgtccgcccg agacgacgag gctgacgtag ggcgggtccg gctcgccgtg
      121 cgcgaggaag ttcgcccaga catgcccctc caggtggtta acgccgatca acggggcgcc
      181 ggtggagagc gagatcgcct tggcggccgc catcccgacg agcagcgcgc cgacgagccc
      241 cggccccacc gtgacggcca caccgtccag gctcttgaac ccgacgcccg cctcgctgag
      301 ggcctgttcg agcagggggt tcagcgactc gacgtgcgct cgagcggcga cctcgggcac
      361 cacccctccg aaccgttcgt gcaggtgctc ctgacgtgcg atcaggttcg cccgcacgtg
      421 gaacccgtcc tccacgaccg ccacggcggt ctcgtcacac gacgtctcga taccgagggt
      481 gatcaccctg ggcgcacgcc ctgcgggagg gtctcctcga tctcgtccag acgccgccgg
      541 tacgactcgg acctcacgtc gtcggtccac atgatgagcg cgtcctcgct cacctcttgg
      601 tagtagttgc gccggatgcc gacgggccgg aagccgaagc gagcgtagat ccgctgcgcc
      661 ccccagttcg tgacacgaac ctcgagggac accgcgcgcg ctcccatctg gatggccccg
      721 gggatcagct ctgccagcaa tcgcgtcgcg atcccgtgcc cttgatgcat cggatcgacg
      781 gccacgttgg tgatgtgcgc ctcgtccccg tagcagatca gtcctccgta cccgacgatc
      841 tccttgtgga ctcgggcaac gagatagcag cggccgttcg gatccgtgat ctccgcggcg
      901 aagaggttcg gtgaccacgg ccgcgcgtag acctggcgct cgatcgccat gacgccgcgc
      961 aggtgccgcc ggcgcatggg ggtcacctca agcgccggtc cttcgatccc ggttgtccca
     1021 cgcgatctcc gcatccgact tcctcaggta catcggcacc acgtcgtgga gccggtcgtg
     1081 ctcctcgcgg aggaaccgcg ggaccgccag ctcgaccagc gccgcggcct ccgggtgcgc
     1141 ccggatcgtc gaggcgaact cgaccctgct ccccagcccc tcgagctggt tccggtacag
     1201 gatggcaccg tcgccgaccg cgagcatctc ccccggcacg gtttccagct cggcgcagag
     1261 gtgatcgggc gtccccaccg tgtattcggt ccctcggacg accccgcccg ggagcgcccg
     1321 gtagatcgcc gagaacacct cccgtcggcg tccgtcgatc accgcgacga tcagcttgtg
     1381 cgtatggcga accgagaacg ccagcgcgtc caggctcgtg atcccgacga tcgggacgct
     1441 gaggacctgg gcgagcgtct tcgcggtctc gatgccgacg cgcagccccg tgaacaaccc
     1501 cggcccgatc ccgacggcga tcccgccgac ctggccgagc tcgacgccgg tccatccgca
     1561 gagttgctcc agcgcaggcg tgaccgcctc ctggcgagcc ttcccggtca ccgagaccgc
     1621 cccgagcagc tcccgctccg agccgatcgc caccgacgtg tgcggcgtcg aggtctcgat
     1681 ccccacgacg atcacggcgc cgcctccgcg accgggtcgt cggatggctc gtcgcggcgg
     1741 aagggttgga ggagctgctc gagccgttcc caccgtagcg cccacgatcg cccgtggggt
     1801 gtgagcacga tccgtcgcgc gtcgtcgtcc gttcccgtgg tgagctgcac gcggagccgg
     1861 tcctcgggca agagctcctg gaccacgtcg ccccactcga cgaggagcac ccggtcgcgg
     1921 ccgcccagct cgtcgagccc gaggtcgacg acgtcttgca cgcgctcgag ccggtacacg
     1981 tcgacatggg cgacgtcgag gctgcccgcg tactccttca ccagtgtgaa cgtgggtgac
     2041 gcgacgtgat cctccacccc gagacctctt ccgatccctt ggaccagcgt cgtcttgccg
     2101 gcgccgagat cccccgtgag gacgatggtg tcgcgaggca gcagcagggt cgcgagggcg
     2161 gcaccgacgt cccgcgttcc gtcggcggta gcggtcgcga gctcgaggcg catgatcaga
     2221 acagctcgag ctgctcctcg ggcgccggcg cgatctcggc gtccggcacg cggaccggcg
     2281 cgggctcggg gacctgaacc ggtgcaggtg tcggctcggg tgccggcgcg gacccgggcg
     2341 ccgggtccat cgccgcgagg gtgtcgcgca cgagctcgaa gtcctcgcgg agctcaccga
     2401 actggcgcga cttctcccca ccgccgtgca ggatcgcggc cggatggaac gtcgggatca
     2461 ccgtccttcc gtgccatgga taggcacgcc cgcgcatccg tgtgatcccc tgctgggtct
     2521 gcaggacgaa cttcgtcgcg aagttgccga gggtcacgat cacacgcggc tggatcgacg
     2581 agatctgttc cacgagccac ggcgtgcagg actcgatctc gtcctcctgc gggtcgcggt
     2641 tcccgggcgg ccggcacttc aggatgttgc agatgtagac gtcgccgcgg ccgaggccga
     2701 ggacgtcgcc gaggaccttc gtgagcaggt gcccggcggc tccgacgaac ggctcaccct
     2761 gcttatcctc gtggaacccg ggcgcttcac cgatgaacag cacgtccgca tccgggttcc
     2821 ccacgccgaa cacgacctgg gtccgcccgc cggcgagccg gcaccgggtg caccccgcgg
     2881 cctcggcacc gatgtccgcc agcgtcctgc tcacgccgcc ccgctcttcc tcgttccctc
     2941 gcgcatccgg ccgcgaccca ctccgaccga gagggcctcc cgcgcgaacg agcggagctc
     3001 cgggttgagc cgttctggcc gttccagcat cgcgatgtga cctgctccct cgacgaggaa
     3061 cagcctcccg ttcgggagcg cggcggcgag ctccacggcg acggcgggcg gcgtcacgcg
     3121 gtcctggtct cccaccacga cgagcgcagg caccgtgatc cgcgcgaggg catggcggaa
     3181 gtccatccgc atcagctccg acaggccgtc cgtccagacg agggaggtcg tgtgctcggc
     3241 gagatgcagc acgtgctcaa cgacgtcatc cggcgcctcg ggggcgaact gcgtcacgcg
     3301 cgcgacgatg gccggcacct ccgccggacc ggcgaggatc gcccggcgga ggcggtccac
     3361 gcggcgagcg gccgtggcga ccgatccgag gcggggtcgt aggaggtggg tcacgccccc
     3421 catcgcgccc cgcaacaggt cggaagcgga cgtcccgagg aacgcgacgc ccccgatccg
     3481 tggaccgaag aggtccgggc gctgctcggc gagggcgagg atcgccatcg cccccatcga
     3541 gtggccgatc accacgacgc gccgatcggg tgcgaccgca tccaggacgg tcgcgaggtc
     3601 tctccccatc gagcggagac tcacgtcgcc ggtccccggc acctcactcc ttccgtgcgc
     3661 acgatggtcg agggcgatgc agcggaactc gtcgccgagg tcggaccact gctcgcgcca
     3721 cgtcgtcatg tcgaggctga acccatggga gaacagcatg atcgggccat ccggcggacc
     3781 ggccgcgcgc acggcgagcg ccgtgccgtc gaaggcctcg atcggcccca gatcctccgg
     3841 gggcggtccc cacgcgcggt cgccgtgcgc cgcgcgccgg tttcgccaga gcgctc
//
LOCUS       DASUVE010000289         2545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1649778, whole genome shotgun sequence.
ACCESSION   DASUVE010000289 DASUVE010000000
VERSION     DASUVE010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649778"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VF036_06250"
     CDS             <1..383
                     /locus_tag="VF036_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX6264864.1"
                     /translation="LEDEAGFFTRDVGPGPEFSHLYPDPMTKVDSRSLAGLAAQLLRP
                     PPTLDLSHVTPIRYTMAEAMTAVERRIEDLGRAASFRDLVADCGERIEVVVRFLALLE
                     LYRDGRVELVQGDTFGEIRVEWQG"
     gene            380..988
                     /gene="scpB"
                     /locus_tag="VF036_06255"
     CDS             380..988
                     /gene="scpB"
                     /locus_tag="VF036_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX6264865.1"
                     /translation="MTMPEPEGVTITSDTRALEALLFVSDEPLASSVLAQALEIERRT
                     VEELCDRLAAELEARGAGVVLRNVAGGWRLFTHPDTAPEVERFVLSSRQARLTKAALE
                     TLAIVAYKQPVTRHQITSIRGVNSDGVLRALEDKGLVEEAGREETPGRPILFATTPSF
                     LERLGLPSLATLPSLAPLLGIEGDTEEADTETPPPEPPEPAG"
     gene            992..1714
                     /locus_tag="VF036_06260"
     CDS             992..1714
                     /locus_tag="VF036_06260"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX6264866.1"
                     /translation="MAVERLQRLLARAGYGSRRACEELIVDGRVTLNGNVATLGDRGD
                     PSKDEVRVDGLEVNLDPNVKYFALHKPAGVVTTMHDPQGRPDIRAYLPAGGPRVFPVG
                     RLDRDTEGLLLLMNDGDLANGLTHPRFGVEKEYLAEVEGLPTQKHVGMLRRGVELDDG
                     HASAANAWVAGRSGERGAVRVVMTEGRKREVRRLLAAAGLPVVRLVRVRVGPVRLGAL
                     GPGERRELTLDEVVALRQVVDG"
     gene            1770..2129
                     /gene="aroH"
                     /locus_tag="VF036_06265"
     CDS             1770..2129
                     /gene="aroH"
                     /locus_tag="VF036_06265"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017566596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX6264867.1"
                     /translation="MRLRAARGAIRVPSDDGDQLLGATERLLSAILERNAVDPDDLVS
                     ILFTATEDLRSAFPAEAARRMGLGRVPLLCAQEIPVEGSMRSVIRVLVHFHSDRTLDE
                     VAHVYLDGAESLRDDVG"
     gene            2142..>2545
                     /locus_tag="VF036_06270"
     CDS             2142..>2545
                     /locus_tag="VF036_06270"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX6264868.1"
                     /translation="MTVGAASAPRPIERVTVVGTGLMGTSVALAAARAGASVIGWDVD
                     PPTTARAAAVGGFTGAATLEEAVEGAELVVVCTPIPMIADAVAAALSTTSEAIVTEVG
                     SIMDSVAKEVAERADPAHLRRFVPGHPMGGSER"
BASE COUNT          360 a          849 c          956 g          380 t
ORIGIN      
        1 gtctcgagga cgaggccggc ttcttcacca gggacgtcgg acccggtccc gagttctcgc
       61 atctgtatcc ggacccgatg acgaaggtgg actcgcggtc gctcgccggc ctcgcggcgc
      121 agctgttgcg accgcccccg acactcgatc tcagccacgt gacgccgatc cgctacacga
      181 tggcggaggc gatgaccgcc gtcgagcgcc ggatcgagga tctcgggcgc gccgcctcct
      241 tccgcgacct cgtcgcggac tgcggggaac ggatcgaggt cgtcgtccgg ttcctcgcgc
      301 tcctcgagct gtaccgtgac ggccgggtgg agctggtgca gggcgacacg ttcggcgaga
      361 tccgcgtcga gtggcaggga tgacgatgcc ggagccggag ggggtaacga tcacctcgga
      421 cacacgagcc ctcgaggcgt tgctgttcgt ctcggacgag ccgctggcct cgtcggtcct
      481 ggcacaagcg ctcgagatcg agcggaggac ggtcgaggag ctgtgcgacc gactcgccgc
      541 ggagctcgaa gcccgcggcg cgggggtcgt gctccgcaac gtggcgggcg gctggcgcct
      601 gttcactcac cccgacaccg ctcccgaggt cgagcgattc gtactgtcgt cgcgtcaggc
      661 ccggctgacg aaggcggcgc tcgagacgct cgcgatcgtc gcgtacaagc agccggtcac
      721 tcggcaccag atcacatcga tccggggtgt caactccgac ggggtcctcc gggcgctcga
      781 ggacaagggc ctcgtcgagg aggcggggcg ggaggagacg ccgggtcggc cgatcctgtt
      841 cgcgacgacg ccgtccttcc tcgaacggct gggtctgccg tcgctggcca ccctgccgtc
      901 gctggcgccg ctcctcggga tcgaggggga caccgaggag gcggacacgg agacccctcc
      961 gccggagcct cccgagcccg ccggataggc agtggcggtc gaacggctgc agcgcctcct
     1021 cgcccgcgcc ggctacggct ctcgacgagc gtgcgaagaa ctgatcgtcg atggtcgcgt
     1081 caccctgaac gggaacgtgg cgacgctcgg ggaccggggt gatccctcca aggatgaggt
     1141 tcgggtcgac ggtctcgagg tcaacctcga cccgaacgtc aagtacttcg ccctccacaa
     1201 gccggcggga gtggtcacaa cgatgcacga cccacagggg cgtcccgaca tccgcgcgta
     1261 cctccccgcg ggcggtcctc gcgtgttccc cgtgggccgg ctggatcggg acaccgaggg
     1321 cctgctcctc ctgatgaacg acggggatct cgcgaacggg ctcacgcatc ctcggttcgg
     1381 cgtcgagaag gagtacctcg ccgaggtgga gggcctcccg acgcagaaac acgtcgggat
     1441 gctgcgacgt ggcgtggaac tggacgacgg tcacgcaagc gcagcgaacg cgtgggtcgc
     1501 cggtcgttcg ggcgaacggg gggccgttcg cgtcgtgatg accgagggac ggaaacgcga
     1561 ggtccgtcgg ctgctggccg cggcagggtt gccggtcgtc cgcctggtcc gcgtgcgggt
     1621 cgggcccgtg cgcctcggcg ccctcggccc cggggagcga cgcgagctca cgctggatga
     1681 ggtggtcgcg ctcaggcagg tcgtcgacgg gtagccttga gaacctcggg gcgtcgcgcc
     1741 cggagccgga acgagcgatg agggtgtcca tgaggcttcg tgcggcgcgc ggcgcgatcc
     1801 gcgtgccatc ggacgacggt gaccagctgc tgggagccac cgagcggctc ctgtcggcca
     1861 tcctggagcg caacgccgtc gaccccgatg acctggtgag catcttgttc acggcgaccg
     1921 aggacctccg gtccgcgttc cccgcggagg ccgcccgtcg gatggggctg ggtcgcgttc
     1981 cgctgctctg tgcgcaggag atcccggtgg agggctcgat gcgctcggtg atccgtgtcc
     2041 tcgtgcattt ccactcggat cggacgctcg acgaggtcgc gcacgtgtac ctggacggcg
     2101 cggagtcgct ccgagatgat gtcggctgaa cgggggcacc gatgacggtc ggcgccgctt
     2161 cagctccgag gccgatcgag cgggttaccg tcgtggggac cggtctgatg ggcacgtcgg
     2221 tggcgctcgc cgcggcgcgt gccggcgcca gcgtgatcgg gtgggacgtc gatccaccga
     2281 cgacggctcg ggcggccgcg gtcggcgggt tcacgggggc cgccacgctc gaggaggccg
     2341 tggagggcgc cgagctcgtc gtggtctgca cgccgatccc gatgatcgcc gacgccgtcg
     2401 ccgccgcgtt gtccacgacc agcgaggcga tcgtcaccga ggtggggagc atcatggact
     2461 ccgtggccaa ggaggtggcc gaacgagccg atccggccca cctgcgccgc ttcgtcccgg
     2521 ggcacccgat gggggggagc gagcg
//
LOCUS       DASUVE010000290         2633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_143439, whole genome shotgun sequence.
ACCESSION   DASUVE010000290 DASUVE010000000
VERSION     DASUVE010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143439"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VF036_06275"
     CDS             complement(<1..405)
                     /locus_tag="VF036_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX6264869.1"
                     /translation="MYGSFASPDLDDDGLIGVADALNRHIASAQVALLGVIAEVARRR
                     AWQDSGARDVAHWFSIRYGMSWWKADRWIKAAAALHDLPGIADALETGVLGIDKVVEL
                     CRFATAENERELIGWASDATCATIRRRADLEVW"
     gene            complement(431..1651)
                     /locus_tag="VF036_06280"
     CDS             complement(431..1651)
                     /locus_tag="VF036_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264870.1"
                     /translation="MDIPERYVWLCLRVGRHLDGFVDAFIGPSEMERAVEAEEAADPN
                     DLRDEGQLLLDGLGDADLSDDRRRWLRGQLEALACVTDRLAGEEITWADEVERCLGVR
                     PSRSDTSELREIHHRLDAVLPGNGSLRERYNAWDANNAVPREKLVPALERLQDVLRPR
                     AHAIAPMPAGESVGYELVSGVPWIAYNRYEGRHRSRVEVNADLPVSVVLLVSLAAHET
                     YPGHHTERVAKDAHLYRDLGRLETSVAISAPESLVSEGIATNALDEALGREPFAVVAD
                     VLADLDLRFDPVEAHEVHEAELALYAPAVNAAYLLHEDGVSTDEVEGYLREWALESNE
                     RAARTVAFLTDPSSRAYVSAYPDGRRLCRDFADRAPGNFTRLLTEQLTTADLRSLSRP
                     FRKDPPDVRSVTAW"
     gene            complement(1657..2130)
                     /locus_tag="VF036_06285"
     CDS             complement(1657..2130)
                     /locus_tag="VF036_06285"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6264871.1"
                     /translation="MSQSGPDRELITEEDRLWSELHDLVDSMPADKVEEPGYFAEGWS
                     AKDLIAHIGSWLAEAGVVLDRLRAGTYRPEEIDIDAMNAQFYGSMHDVAFHDVRAQAF
                     TARNRMLRAWRSLPEGSPEADRWIRKSGPEHYAEHIPRLRDWVGEVRRKEPGPRE"
     gene            complement(2189..>2633)
                     /locus_tag="VF036_06290"
     CDS             complement(2189..>2633)
                     /locus_tag="VF036_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX6264872.1"
                     /translation="DFLRATRPLIDVITALRGGFAKYHVDERLQDHLRDVEDHLIQII
                     ERVDEFRVALRDMLTVNATLVAQRQNEEMRNLAEAANSQNEEVKKISAWAAILFAPTL
                     IGTVYGMNFVHMPELDWILGYPFALLLMAVTCVGLYALFKRRRWL"
BASE COUNT          422 a          908 c          883 g          420 t
ORIGIN      
        1 ccatacctcg aggtccgcgc gacgacggat cgtcgcgcac gtggcgtcgc tcgcccaccc
       61 gatgagctca cgctcgttct cggccgtggc gaaccggcac agctcgacca ccttgtcgat
      121 accgagcacg ccggtctcca gcgcgtcggc gatacccggc agatcgtgga gcgccgcggc
      181 cgccttgatc caccggtccg ccttccacca ggacatgccg tagcggatgg agaaccagtg
      241 cgccacgtcc cgagcgcccg agtcctgcca cgcccgccgc cgagcgacct cggcgatgac
      301 gccgagtagc gccacctgcg ccgacgcaat gtggcggttc agcgcatcgg cgacgccgat
      361 caatccgtcg tcgtccagat cggggctcgc gaaggacccg tacatgtgtt cgaacgtatg
      421 gggaagtccg tcaccacgca gtgaccgatc gcacatccgg cggatctttc cggaagggtc
      481 ggctcaggga acggaggtcg gcggtggtga gctgctccgt caggagccgt gtgaagttcc
      541 cgggcgcccg atcggcgaag tcccggcaga ggcgccgccc atccggatag gcggacacgt
      601 acgcgcggga cgacggatcg gtgaggaacg cgacggtccg cgccgctcgc tcgttcgatt
      661 cgagcgccca ctcgcgcagg tatccctcca cctcgtccgt cgacacgccg tcctcgtgga
      721 gcaggtaggc ggcgttcacc gccggcgcgt acagggcgag ctctgcctca tggacctcgt
      781 gcgcttcgac cggatcgaac cggaggtcga ggtctgccag gacgtccgcg acgaccgcga
      841 agggctcacg accgagcgcc tcatcgaggg cgttcgtcgc gatcccttcg gagacgaggg
      901 actcgggggc cgagatcgcg acgctcgtct ccagacggcc gaggtctcgg tacaggtggg
      961 catccttcgc cacgcgctcg gtgtgatgac cgggatacgt ctcgtgggcg gcgagggata
     1021 cgagcagcac gacggagacc gggagatcgg cgttgacctc gacccggctc cggtgccgac
     1081 cctcgtaccg gttgtaggcg atccacggca cgcccgagac gagctcgtag cccacggact
     1141 ccccggcggg catcggcgcg atcgcgtgcg cccgggggcg caggacgtct tgcaagcgct
     1201 cgagcgcggg aacgagcttc tcgcgtggaa ccgcgttgtt cgcgtcccag gcgttgtatc
     1261 gctcgcggag cgagccgttc cccgggagga ctgcgtccag ccgatggtgg atctctcgca
     1321 actcgctcgt gtcggaccgt gatgggcgga caccgaggca tcgttcgacc tcgtccgccc
     1381 aggtgatctc ctcaccggcg agccggtcgg tgacgcacgc gagcgcttcg agctggccgc
     1441 gcaaccatcg ccgacggtcg tccgagaggt cagcgtcgcc gagaccgtcg aggaggagct
     1501 gcccctcgtc gcggaggtcg ttcgggtcgg cggcctcctc ggcctcgacc gcccgctcca
     1561 tctccgatgg gccgatgaag gcgtcgacga acccatcgag atgacggccc acccgcaggc
     1621 agagccacac gtatcgctcc ggtatgtcca cggagatcac tcccggggac cgggttcctt
     1681 ccggcgaacc tcccccaccc agtcccggag tctcgggatg tgctccgcgt aatgctcggg
     1741 acccgacttc cggatccagc gatcggcttc gggcgatccc tccggcagtg agcgccacgc
     1801 ccggagcatc cggttgcggg ccgtgaacgc ctgcgcgcgt acgtcgtgga acgcgacgtc
     1861 gtgcatcgag ccgtagaact gcgcgttcat cgcgtcgatg tcgatctcct cgggtcgata
     1921 cgtgccggct cggaggcggt ccaggacgac cccggcctcg gccagccagc tcccgatgtg
     1981 cgcgatcaga tccttcgcgg accacccctc ggcgaagtag ccgggttcct cgaccttgtc
     2041 cgcgggcatc gagtcgacca ggtcgtgcag ctcgctccac aaccgatcct cttcggtgat
     2101 cagctcccga tccggacccg actgcgacac gcgagccagt atgacgcgcg ggccctccga
     2161 cgacgtcgct cgtcgtcgcc aacgacgcct agagccaacg cctccgcttg aacagcgcgt
     2221 agaggccgac acacgtcacg gccatgagca ggagcgcgaa cgggtagccg aggatccaat
     2281 cgagctcggg catgtgaacg aagttcatcc cgtagacggt cccgatgagc gtgggtgcga
     2341 acaggatcgc ggcccacgcg gagatcttct tgacctcctc gttctgggag ttcgcggctt
     2401 ccgccagatt gcgcatctcc tcgttctgcc gttgcgcgac gagcgtggcg ttcaccgtga
     2461 gcatgtcgcg gagggccacc cggaactcgt cgacgcgctc gatgatctgg atcaggtggt
     2521 cttcgacatc gcggagatgg tcttggaggc gctcatcgac gtgatacttg gcgaatcccc
     2581 cgcggagcgc cgtgatgacg tcgatcaagg ggcgagtcgc acgcaggaag tcg
//
LOCUS       DASUVE010000291         2409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1462743, whole genome shotgun sequence.
ACCESSION   DASUVE010000291 DASUVE010000000
VERSION     DASUVE010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462743"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1557)
                     /gene="infB"
                     /locus_tag="VF036_06295"
     CDS             complement(<1..1557)
                     /gene="infB"
                     /locus_tag="VF036_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020523441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX6264873.1"
                     /translation="MRIHELAKELGVSSKELLETLTKMGVAGKSASSSVPEDLVPRLR
                     ASGGKVTKPAKPRQVMEPPPKPRPKVKPKAKAAPKVVAVPDAEAVPTEEPDGAPEPAA
                     AATAVPVTPAPLTAVPDAAVSAAPPAAPAGPILQVVRGSTPQIFAEKTNRSPADIVKI
                     LFMAGEMVTATTSLSDEALQTLGSELGYHVEIVGMEDELQAEELEEEVDESKLVPRSP
                     VVTVMGHVDHGKTKLLDAIRTTDVVSGEFGGITQHIGAYQAHVNGREITFIDTPGHEA
                     FTAMRARGAQITDIAVLVVAADDGVMPQTVEALDHAKAAGVPVVVAVNKIDKEEADPN
                     RVRTQMVERGLVPSEWGGDTEFVDVSAKTSANLEALLETVLIVADLAELKGDPTGRAR
                     GTVVEAHLDKGRGPVATVLVQKGTLEVGDSLVCGITHCKVRAMVDENGQPVDVAGPAK
                     PAQVLGWNGVPSVGDEFREVDDEREARHIASEREARVRSAEVVNQRPASLTELLRRQE
                     RQEIPELNLVV"
     gene            complement(1554..1823)
                     /locus_tag="VF036_06300"
     CDS             complement(1554..1823)
                     /locus_tag="VF036_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006375269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEX6264874.1"
                     /translation="MAREPERSCVGCRKRAPKADLLRIVRSAAGVRVDLIGSASGRGA
                     YVHRDPACADAAVRRGTVASALRSGLTQEELARLRNEIEEASQAI"
     gene            complement(1830..>2409)
                     /locus_tag="VF036_06305"
     CDS             complement(1830..>2409)
                     /locus_tag="VF036_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264875.1"
                     /translation="GHRSKIAVASNEPAVDPVGACVGPKGSRVRMVVNELRGEKIDVV
                     PWSPNPAEFVANALQPAKVREVTVNPETQTALVIVPDYQLSLAIGREGQNARLAARLT
                     GWRIDIKSESQAAEPAPAAAPASVEQPAAVEPPELESGAAASETSSTETPDDEDAQAA
                     TVEEVATGAGAEPTPEESEPVEAVPAAPAEAE"
BASE COUNT          311 a          881 c          812 g          405 t
ORIGIN      
        1 caccacgagg ttcaactcgg ggatctcctg gcgctcctgg cgccggagga gctccgtgag
       61 cgacgcgggc cgctggttga ccacctcggc cgatcgaacg cgtgcttcgc gttcggatgc
      121 gatgtgacgc gcctcgcgtt cgtcgtcgac ctcgcggaac tcgtccccga cgctggggac
      181 cccgttccag ccgagcacct gggcaggctt cgccggtccg gccacgtcga cgggctgccc
      241 gttctcgtcg accatcgcac gcaccttgca gtgcgtgatg ccgcagacca gggagtcccc
      301 gacctcgagg gttcccttct gcacgaggac ggtcgccacg gggccacgac ccttgtcgag
      361 gtgtgcctcc acgacggtcc ctcgcgcgcg gccggtggga tcgcccttga gctccgcgag
      421 gtcggcgacg atcagaacgg tctcgagcaa ggcctcgagg ttcgcgctcg tcttggccga
      481 gacgtcgacg aactcggtgt cgcctcccca ctccgacggc acgagacccc gctcgaccat
      541 ctgggtccgc acgcggttcg gatccgcctc ctccttgtcg atcttgttga ccgccaccac
      601 cacgggcaca ccggccgcct tcgcgtggtc gagcgcctcg acggtctgtg gcatcacgcc
      661 gtcgtcggcg gcgacgacga gcaccgcgat gtccgtgatc tgggcgcccc gggcacgcat
      721 cgcggtgaag gcctcgtggc cgggtgtatc gatgaaggtg atctccctgc cgttgacgtg
      781 cgcctggtac gcgccgatgt gctgggtgat cccgccgaac tcgccggaga cgacgtcggt
      841 cgtacggatc gcgtcgagga gcttggtctt cccgtggtcg acgtgtccca tcacggtgac
      901 caccggcgag cggggcacga gcttggactc gtcgacctct tcctcgagct cctcggcctg
      961 gagctcgtcc tccatcccga cgatctccac gtgatagccg agctcggaac ccagggtctg
     1021 gagggcctcg tcggacagcg acgtcgtcgc ggtgaccatc tcccccgcca tgaacaggat
     1081 cttcacgatg tccgccggtg atcggttcgt cttctccgcg aagatctgcg gcgtcgaacc
     1141 ccggacgacc tgcaggatcg gcccggcggg agcagccggg ggtgcggcgg acaccgcggc
     1201 atcggggacg gcggtcaagg gagcgggggt taccggcacg gcggtggccg cagccgccgg
     1261 ctcaggggcg ccatcgggct cctcggtcgg aaccgcctcc gcgtcgggga cggcgacgac
     1321 cttgggggcc gccttcgcct tcggcttgac cttggggcga ggcttcggcg gcggttccat
     1381 gacctggcgc ggcttcgcgg gcttcgtgac cttgccgccc gaagcacgga ggcgcggcac
     1441 gaggtcttcg ggcacgctgg aggaggcgga cttccccgcc acacccatct tcgtgagcgt
     1501 ctccagcagt tccttgctcg agacgccgag ctccttcgcg agctcgtgga tcctcatatc
     1561 gcctgtgacg cctcctcgat ctcgttccta agcctagcga gttcctcttg cgtcaacccc
     1621 gagcgcagcg cgctcgccac cgtgccgcgc cggaccgcag cgtcggcgca cgcggggtct
     1681 cgatgaacgt acgcaccgcg accggacgca gacccgatca ggtctacccg caccccggcc
     1741 gccgaccgga cgatgcgaag cagatcagcc ttcggtgcgc gcttccgaca cccgacgcac
     1801 gaccgctccg gctcgcgcgc catggttcct cattccgcct ccgcgggcgc ggccgggacc
     1861 gcttcaacgg gctccgactc ctcgggggtc ggctcggcgc cggcgccggt ggccacctcc
     1921 tccaccgtcg ctgcctgcgc gtcttcgtcg tccggagtct ccgtcgagct cgtctcggat
     1981 gcggccgctc cgctctccag ctcaggtggc tcgaccgcag caggttgctc gaccgaggcc
     2041 ggggccgccg cgggggcggg ctcggcggcc tggctctcgg acttgatgtc gatccgccac
     2101 ccggtcagtc gtgccgcgag ccgggcgttc tgaccctccc ggccgatcgc gagcgacagc
     2161 tggtagtcgg gcacgatgac gagagccgtc tgggtctcgg ggttgacggt gacttcccgg
     2221 accttcgccg gctgcagcgc gttcgcgacg aactccgcgg ggttcgggct ccacggaacg
     2281 acgtcgatct tctccccgcg gagctcgttc accaccatcc ggacacgcga ccccttgggc
     2341 ccgacgcatg ccccgaccgg gtcgaccgcc ggctcgttcg aggcgaccgc gatcttggaa
     2401 cgatggccg
//
LOCUS       DASUVE010000292         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1650373, whole genome shotgun sequence.
ACCESSION   DASUVE010000292 DASUVE010000000
VERSION     DASUVE010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650373"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..>2178)
                     /locus_tag="VF036_06310"
     CDS             complement(91..>2178)
                     /locus_tag="VF036_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019812063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6264876.1"
                     /translation="VGVVDSLRAGTIMRERGIESGGLTVLPNTEVHSLGVEGGRIRRV
                     ETSKGTIEAETVVVACGVWSPLLAGMAGAHIPLVPMVHQMIDIGPVPRFEKSSRAIEF
                     PIVRDMDVFMYERQDGTSLEIGSYAHRSILHDPEEIPSIEAAALSPTEFPFTQADFDP
                     QMVDALDLMPEIVGDESVGVKYAINGLISLTPDGMPILGETPEVKGLWSAAAVWVKEG
                     PGVGKSLAEWMVHGESHIDLHSSDVARFHDHQKTRSHVKARTTEAFPKTYGIIHPAEQ
                     WSSDRNLRLSPMHERERELGAVFYEVAGWERPHWYEANAPLVERYGIESREAEWDARW
                     WSPIVNAEHLAMREHAGLFDLSAFAIFDVNGPGALDALQRSVLAQMDVAVGRVVYTPV
                     LSPGGGFRSDLTIMRLGDEEFRIVTGGAHGMADRKLFSDQLPDDGTATLVDVTTGWTT
                     IGLWGPRARDILSSVTADDVSHEGFPFATCRTVEIGSQLVLASRISYVGDLGWELYVP
                     IEQGARLWDALWEAGEPHGLTACGIGVYGMTGRLEKCYRAFAAELESEYTVVEAGMAR
                     PTVKEQDFIGRDAHLRHREEEPAAILCTLTVDDHTSASGVERYPLGREPITLRDGTPL
                     TDAKGRRSYVTSAGAGPSLGKHILLAYLPPERAVEGSTDLAVEYMAERYPVTVDVVGS
                     RPIFDPENTRIRS"
BASE COUNT          315 a          777 c          750 g          336 t
ORIGIN      
        1 caaggtggcg agtctcgatc gcccgaccgt cgtccgtgag gacgattgtg ccccccgtca
       61 tcgggacgcg cttcacgcag acgagggccc tcacgagcgg atcctcgtgt tttcggggtc
      121 gaagatcggc cgcgacccga cgacgtccac ggtcaccgga taccgctcgg ccatgtactc
      181 gaccgcgagg tcggtcgagc cctcgaccgc gcgctcgggc ggcaggtagg ccagcaggat
      241 gtgcttgccg agtgacgggc cggcgcccgc actggtgacg taggatcggc ggcccttcgc
      301 gtccgtcagc ggcgtgccgt cgcggagggt gatcggctcc cgtccgagcg ggtaccgctc
      361 gactccgctc gcggaggtgt ggtcgtcgac tgtcagcgtg cagaggatcg cggccggctc
      421 ctcctcgcgg tgccggaggt gggcgtccct gccgatgaag tcctgctcct tcacggtcgg
      481 ccgcgccatg cccgcctcga cgaccgtgta ttcgctctcg agctccgcgg cgaacgcccg
      541 gtagcacttc tcgaggcggc ccgtcatccc gtagacgccg atcccgcagg cggtgagccc
      601 gtgcggctcg cccgcctccc acagggcgtc ccagagccgc gcgccctgct cgatcgggac
      661 atacagttcc cagccgaggt cacccacgta ggagattcgg gacgcaagga cgagctgcga
      721 gccgatctcg accgtccggc aggtggcgaa ggggaagccc tcgtgcgaga cgtcgtctgc
      781 ggtgacgctc gagaggatgt cccgcgcccg tggcccccag agcccgatcg tcgtccagcc
      841 cgtcgtcacg tcgacgagcg tcgcggtgcc gtcgtccggc agctgatccg agaagagctt
      901 gcggtcggcc atcccgtgcg cgccgccggt gacgatccgg aactcctcgt cgccgagccg
      961 catgatcgtg aggtcggagc ggaagccgcc gcccggcgag agcaccggcg tgtagacgac
     1021 gcgcccgacc gcgacgtcca tctgcgcgag cacgctgcgc tggagcgcgt cgagcgcccc
     1081 ggggccgttc acgtcgaaga tcgcgaaggc cgagagatcg aacagtccgg cgtgctcgcg
     1141 catcgcgagg tgctccgcgt tgacgatcgg cgaccaccag cgggcgtccc actcggcttc
     1201 gcgggactcg atcccgtagc gctcgacgag cggggcgttc gcctcgtacc agtgggggcg
     1261 ctcccagccc gccacctcgt agaacaccgc cccgagctcc ctttcccgct cgtgcatcgg
     1321 cgagagtcgg agattgcgat ccgacgacca ctgctcggcc ggatggatga tcccgtacgt
     1381 cttcgggaac gcctcggtcg tccgggcctt cacgtgcgag cgggtcttct ggtggtcgtg
     1441 gaaccgcgcg acgtcggagg agtggaggtc gatgtgcgac tcgccgtgca ccatccactc
     1501 cgcgagggac ttgccgacac cgggcccctc cttgacccac accgcggccg ccgaccagag
     1561 ccccttcacc tccggcgtct cgccgaggat cggcatgccg tccggggtga gcgagatgag
     1621 cccgttgatc gcgtacttca cgccgaccga ctcgtcgccg acgatctcgg gcatcaggtc
     1681 gagcgcgtcg accatctgcg ggtcgaaatc ggcctgggtg aacgggaact ccgtcggcga
     1741 cagcgcggcg gcctcgatcg aggggatctc ctcggggtcg tgcaggatcg agcggtgcgc
     1801 gtaggagccg atctcgaggc tcgtgccgtc ctggcgctcg tacatgaaca cgtccatgtc
     1861 gcgcacgatc gggaactcga tcgcgcgcga gctcttctcg aagcgcggca ccggcccgat
     1921 gtcgatcatc tggtgcacca tcgggacgag cgggatgtgc gcgcccgcca tgccggcgag
     1981 caacgggctc cagacgccgc aggcgaccac gactgtctcc gcctcgatcg tgcccttcga
     2041 cgtctccacg cggcggatcc ggccgccctc gactcccagc gagtggacct cggtgttcgg
     2101 gaggacggtg agcccgccgg actcgatgcc gcgctcgcgc atgatcgtcc ccgcccgaag
     2161 cgagtcgacg accccgac
//
LOCUS       DASUVE010000293         2770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_897362, whole genome shotgun sequence.
ACCESSION   DASUVE010000293 DASUVE010000000
VERSION     DASUVE010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897362"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..487)
                     /locus_tag="VF036_06315"
     CDS             complement(17..487)
                     /locus_tag="VF036_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX6264877.1"
                     /translation="MTATDGGVVTVIGIGNRFRRDDAAGLEVARKVRERVDGDVRIVE
                     LEGEPLDALDVWTPADEVIVADAVADAGAAGTVHLLDARLEPIPSPFRAKGTHTFSLA
                     DVVELARATERLPRSLLVYGIEGGDFSIGEGLTEPVARAVEDSAAAIVRQVATR"
     gene            complement(484..1797)
                     /locus_tag="VF036_06320"
     CDS             complement(484..1797)
                     /locus_tag="VF036_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEX6264878.1"
                     /translation="MTHGSSPSAETAVHVPGLARVEGEGGLEIRVRGGKVVEARLNIY
                     EPPRFFEAFLRGRRHTEPPDITARICGICPIAYQMSACRAIEDACGVTVDGPIATLRR
                     LIYLGEWIESHALHVYLLHAPDFLGYESAVHMAADHREVVEQGLAMKKAGNGLMDVIG
                     GRSVHPINVRVGGFYRAPTREELRTLLEPLERAREIAVETARWVSGFDFPPFERDYGF
                     VSLRHPTDYPIIEGRIASSDGLDIAVAEYDEHFVEEHVPHSTALHSRVRSTGDHYLVG
                     PLARFNLNRDRLRPLARRAAEEAGIADGCRNPFKSIILRAVELVHACDEAIDIIGSYE
                     PPDPPAVEVPPRAGAGTGATEAPRGLLYHRYVLDPDGVIEDAWIVPPTSQNQGTIEED
                     LVHFADDRLSLEREALTWQCEQAVRNHDPCISCATHFLDLWVVEG"
     gene            complement(1784..2560)
                     /locus_tag="VF036_06325"
     CDS             complement(1784..2560)
                     /locus_tag="VF036_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX6264879.1"
                     /translation="MAARPRLAVWKFASCDGCQLTLLNCEDELLPVAGELEIASFPEA
                     SRAVIDGPYDLSLVEGSITTPSDAERIQEVRRQSRFLVTIGACATSGGIQALRNFKDV
                     DDFMRIVYAQPEYIDTLETSTAIADHVPVDFELRGCPIDRGQLLEVVGAFLNGRKPEV
                     PNHSVCIECKRRGTVCVMVAQGTACLGPVTHAGCGALCPSYDRGCYGCFGPMETPNTT
                     SLGRRFLQLGVRGDDVVRVFRTFNANAEAFREESEARDAR"
     gene            complement(2560..>2770)
                     /locus_tag="VF036_06330"
     CDS             complement(2560..>2770)
                     /locus_tag="VF036_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit gamma"
                     /protein_id="HEX6264880.1"
                     /translation="GPEVMMRFTVQALLDRGVAADRILVSLERNMQCAIKQCGHCQLG
                     PFFMCADGPVMPYALVAPWLRPREI"
BASE COUNT          408 a          991 c          956 g          415 t
ORIGIN      
        1 atcgctccca ggcacatcag cgcgtggcca cctgtcgcac gatggcggcc gccgagtcct
       61 cgaccgcacg cgccaccggc tccgtcagcc cctcaccgat cgagaagtcg ccaccctcga
      121 tcccgtagac gaggagcgag cgcgggagcc gttcggtggc ccgagccagc tccacgacgt
      181 cggcgaggct gaaggtgtgg gtccccttcg cacggaaggg cgatgggatc ggctcgagtc
      241 gggcgtcgag cagatgcacg gtccccgccg cgcccgcgtc ggctaccgcg tccgccacga
      301 tcacctcgtc ggcgggtgtc cagacgtcca gcgcgtcgag cggctcgccc tcgagctcga
      361 cgatccgcac gtcgccgtcg acgcgttccc ggaccttccg agccacctcc aggcccgcgg
      421 cgtcgtcccg ccggaaccgg ttcccgatcc cgatcaccgt gacgacgccg ccgtcggtcg
      481 cggtcatccc tccaccaccc agaggtcgag gaagtgcgtc gcgcacgaga tgcaggggtc
      541 gtggttccgg accgcctgct cgcactgcca cgtgagcgcc tctcgttcga gcgagaggcg
      601 gtcgtccgcg aagtggacga ggtcctcctc gatcgttccc tggttctgtg acgtgggcgg
      661 gacgatccac gcgtcctcga tcaccccgtc ggggtcgagc acgtaccggt ggtacagcag
      721 gccgcgggga gcctcggtgg cccccgtgcc ggcgccggcc ctcggcggca cctcgaccgc
      781 gggcgggtcc ggcggctcgt acgacccgat gatgtcgatc gcctcgtcgc acgcgtgcac
      841 gagctcgacg gcgcgcagga tgatgctctt gaacgggttc cggcagccgt cggcgatccc
      901 cgcttcctcg gccgcgcggc gggcgagcgg gcggagacga tccctgttca ggttgaaccg
      961 cgcgagtggg ccaacgaggt agtggtcacc ggtcgaccgc actcgcgagt ggagcgccgt
     1021 cgagtgcggc acgtgctcct ccacgaagtg ctcgtcgtac tcggccaccg cgatgtcgag
     1081 gccgtcgctc gaggcgatcc ggccctcgat gatcgggtag tccgtcggat gtcgcaacga
     1141 gacgaagccg tagtcccgtt cgaacggagg gaagtcgaac cccgacaccc atcgggccgt
     1201 ctccacggcg atctcgcggg cccgttcgag gggttccagc agcgttcgga gttcctcgcg
     1261 ggtcggggcg cggtagaagc cgccgacccg gacgttgatc gggtggaccg agcgccctcc
     1321 gatcacgtcc atcaacccgt tgccggcctt cttcatcgcg aggccctgct cgaccacctc
     1381 ccgatggtcg gcggccatgt gaaccgcgct ctcgtagccg aggaaatcgg gggcgtgcag
     1441 caggtacacg tgcagggcgt ggctctcgat ccactcaccg aggtagatca ggcgccgcag
     1501 cgtcgcgatc ggtccgtcca ccgtcacgcc gcaggcgtcc tcgatcgccc ggcacgcgct
     1561 catctgatag gcgatcgggc agatcccgca gatccgggcg gtgatgtcgg gaggttcggt
     1621 gtggcgccgt ccccggagga acgcctcgaa gaaccggggc ggctcgtaga tgttcagccg
     1681 agcctccacc accttgccgc cgcgcacgcg gatctcgagc ccgccctcgc cctccacgcg
     1741 ggcgaggccg ggcacgtgga cggcggtttc ggcgctcggt gagctaccgt gcgtcacggg
     1801 cttcgctctc ctcgcggaac gcctcggcgt tggcgttgaa cgtgcggaac acgcgcacga
     1861 cgtcgtcccc ccggaccccc agctgcagga accggcgccc gagcgaggtc gtgttcggcg
     1921 tctccatcgg gccgaagcac ccgtagcagc cccggtcgta cgaggggcag agcgcgccgc
     1981 agccggcatg cgtcaccggg ccgaggcacg cggtgccctg cgcgaccatc acgcagacgg
     2041 tcccgcgtcg cttgcactcg atgcagacgc tgtggttggg cacctcgggc ttccgtccgt
     2101 tcaggaacgc cccgaccacc tcgagcagct gaccccggtc gatcggacac ccgcgcagct
     2161 cgaagtcgac gggcacgtga tccgcgatcg ccgtcgaggt ctcgagcgtg tcgatgtact
     2221 cgggctgcgc gtagacgatc cgcatgaagt cgtcgacgtc cttgaagttc cggagcgcct
     2281 ggatcccgcc cgatgtggcg caggcgccga tcgtcacgag gaacctcgac tgccggcgga
     2341 cctcctggat ccgctcggcg tcggacggcg tggtgatcga gccttcgacg agcgagaggt
     2401 cgtacgggcc gtcgatcacg gcgcgtgacg cctcggggaa cgaggcgatc tccagctcgc
     2461 cggccaccgg gaggagctcg tcctcgcagt tcagcagggt cagctggcac ccgtcgcacg
     2521 aggcgaactt ccacacggcc agacgtggtc gcgccgccat cagatctccc tcgggcggag
     2581 ccagggcgcg acgagcgcgt acggcatcac cgggccgtcc gcgcacatga agaagggacc
     2641 gagctggcag tgcccgcact gcttgatcgc gcactgcatg ttccgttcga gcgacacgag
     2701 gatccggtcg gcggcgacgc ctcggtcgag gagcgcctgg accgtgaacc gcatcatgac
     2761 ctcgggcccg
//
LOCUS       DASUVE010000294         2842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1650960, whole genome shotgun sequence.
ACCESSION   DASUVE010000294 DASUVE010000000
VERSION     DASUVE010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650960"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VF036_06335"
     CDS             complement(<1..519)
                     /locus_tag="VF036_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264881.1"
                     /translation="MRRRLVWGLTALATALAFPVPASSRAPADGAPKIHFAGIDRSVA
                     PESGPIQIPRLVRPGSDRRLDGDGGGGATRPAASPLAPIVAVPAFDGVPDQAGNPVSP
                     GDPTGALGVDYHLAAVNIRMGFFDRAGAPLDPPRFLESLDVALPGAAESFDPKAVYDP
                     YRQHFVLVFLSAT"
     gene            857..2308
                     /locus_tag="VF036_06340"
     CDS             857..2308
                     /locus_tag="VF036_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX6264882.1"
                     /translation="MVPVWAELLADVSTPVGLFPTVAGDGSGILLESVERSERWGRYS
                     FVAGDPAATVVLDADGLHINDAVREVPLQPLRSGSPRDALVAAADAVRGPRLPDLPPL
                     TGGLMGYLAYEAATLLDGHPVPHPTEAPCPPVGLLVIDRAVVFDHWRQRLILVAHVPR
                     GDYRGGVAALEELAGRIAEATTPPLAAVDGPDATGTGDPNMSDEDYRRIVAAFQEHIR
                     AGDIYQGVPSRRVSFDAAAGGFPIYRRLRVTNPAPYMFFLRMLGPEIGPEIKMELAGS
                     SPEPLVRVEGRRVFTRPIAGTRPRGQTDVRDRLLEHELLADPKEQAEHAMLVDLARND
                     LGRVCVPGSVGPTELMQVERFSKVMHIVSTVEGELREDRHPLDALAATFPAGTVTGAP
                     KRRAMELIADNEPTARGPYAGAVGYLTFAGDLDFCITIRTAVVMDERVHVQSGAGVVV
                     DSDPQAELRETKDKAEALLPAIGTLGPELGQTR"
     gene            2314..>2842
                     /locus_tag="VF036_06345"
     CDS             2314..>2842
                     /locus_tag="VF036_06345"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6264883.1"
                     /translation="MDPTRIVRQGYDDLDDTYRRWVEHTRGGYRSTFLARILEEVAEG
                     ADVLEIGCGPGTDAAALAEGRRYVGIDLSDVQLTHARVAAPAGRFLHGDVLDAAFPPA
                     SFDAVVSFYAFNHIPQDRMELLFRRIRSWLRPRGRLFGSFGTSDNPGEVEPAWLGKAD
                     MYFSSLPVHRTGSLLE"
BASE COUNT          431 a          981 c         1014 g          416 t
ORIGIN      
        1 ggtcgcggac aggaacacga ggacgaagtg ctgtcggtag gggtcgtaga ccgccttggg
       61 atcgaacgat tccgccgccc ccggcagggc gacgtcgagg ctctcgagga acctcggtgg
      121 atcgaggggg gcgccggcgc ggtcgaagaa gcccatcctg atgttgaccg ccgccaggtg
      181 atagtcgacc cccagtgcgc ctgtgggatc tcccggcgaa accgggttcc cggcctgatc
      241 gggaacaccg tcgaacgccg gcaccgcgac gatcggagcg agcggcgacg cggccggccg
      301 cgtcgcgccg ccaccgccat cgccgtcgag tcgtcgatcg ctccccggcc gcacgagccg
      361 cgggatctgg atcggtccgg actcgggcgc caccgaccga tcgatcccgg cgaagtggat
      421 cttcggtgca ccgtcggcgg gagccctgga cgatgcgggg accgggaacg cgagcgcggt
      481 ggcgagtgcg gtgagccccc agacgaggcg acgacgcatc gggacatccc tcctgatcgt
      541 gtgcgacgcc ctcccgccgc atgagcggct caacgtagca ggctccgttg actgatgggg
      601 aatccgggtg ggaccatcgc gtccacgatg tcgagggctc ggggcgcctg gacgtggccg
      661 agcggctcga aggggttgtg gcgccctcgg gccccgggac cgccgcgcta gttcccctcg
      721 accgatcgcg acgagccccg ggcatcccac ccggggttct tccgtcccca ggcacgatga
      781 ggagcgaaca ctgagaccga acctccagat ccggccggac cgagccacgt tcgacgcgtt
      841 cgcgcgcgac tgggagatgg tgcccgtctg ggcggagctc ctcgccgacg tctcgacacc
      901 ggtcggcctg ttccccacgg tggccggcga cggatccggc atcctgctgg agtcggtgga
      961 gcggtcggag cggtggggac gatactcctt cgtcgccggc gacccggccg cgacggtcgt
     1021 tctggacgcc gacggcctgc atatcaacga cgccgtacga gaggtgcccc tccaacccct
     1081 gcgctcgggt tcgccgcgcg acgccctggt cgcggcggcg gacgcggtcc gcggccctcg
     1141 tctccctgat ctccccccgc tcaccggcgg gctgatgggc tacctcgcct acgaggcggc
     1201 cacgctgctc gatgggcacc cggtgccaca tccgaccgag gcgccgtgcc cgccggtcgg
     1261 cctgctggtg atcgaccggg ctgtggtgtt cgaccactgg cggcagcgcc tcatcctcgt
     1321 ggcacacgtc ccgaggggcg attaccgcgg gggcgtcgcg gccctcgagg agctcgctgg
     1381 gcggatcgcg gaggccacga cgcccccgct cgccgcggtg gacggccccg acgccacagg
     1441 gaccggcgac ccgaacatgt cggacgagga ctaccggcgg atcgtcgctg ccttccaaga
     1501 gcacatccgg gcaggggaca tctatcaggg cgttccgtcg cggcgggtct ccttcgacgc
     1561 cgctgccggg ggattcccga tctaccgccg gttgcgagtc acgaacccgg cgccctacat
     1621 gttcttcctc cggatgctcg gcccagagat cgggccggag atcaagatgg agctcgccgg
     1681 ctcatcaccg gaaccgctcg ttcgggtcga gggacgacgt gtgttcacgc gtccgatcgc
     1741 gggcacgcga ccgcgggggc agaccgacgt ccgcgaccgt ctcctcgagc acgagctgtt
     1801 ggccgatccc aaggaacagg cggagcacgc gatgctcgtc gacctcgccc gcaacgatct
     1861 cgggcgcgtg tgtgtgccgg gcagcgtcgg accgaccgag ctgatgcagg tcgagcgctt
     1921 ctcgaaggtg atgcacatcg tgtccaccgt cgagggtgag ctccgcgaag atcggcaccc
     1981 gctcgacgcg ctcgccgcga cgttcccggc cgggacggtc accggcgcgc cgaagcggag
     2041 agcgatggag ctgatcgcag acaacgaacc gaccgcgagg ggcccctacg ccggagcggt
     2101 cggctacctc acgttcgcgg gtgacctcga cttctgcatc acgatcagga ccgcggtcgt
     2161 gatggacgaa cgcgtacacg tgcagtcggg cgccggcgtg gtcgtggact ccgaccccca
     2221 agccgagctc cgtgagacga aggacaaggc ggaggcgctc cttcctgcca tcggtacgct
     2281 cggaccggag ctgggacaga ctcggtgagg accatggatc cgacgcgcat cgtccggcaa
     2341 ggatacgacg acctcgacga cacctaccga cggtgggtgg agcacacgcg aggcggctac
     2401 cgatcgacgt tcctcgccag gatcctggag gaggttgccg agggtgccga cgtactggag
     2461 atcggctgcg ggcccggcac ggatgctgcg gcgctcgccg aggggcggcg atacgtgggg
     2521 atcgacctct cggacgtcca gctcacacac gcccgagtgg ccgcgccggc gggacggttc
     2581 cttcacggcg acgtcctgga cgccgcgttc cccccggcga gcttcgacgc cgtggtgagc
     2641 ttctacgcct tcaaccacat cccacaggat cggatggagc tgttgttccg tcgcatcagg
     2701 tcctggcttc gaccccgagg caggctgttc ggctcgttcg gcacgtcgga caacccgggc
     2761 gaggtggagc cggcatggtt ggggaaggcg gacatgtact tctcgagtct gcccgtccat
     2821 cggaccgggt cgctgctcga gg
//
LOCUS       DASUVE010000295         2683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_898227, whole genome shotgun sequence.
ACCESSION   DASUVE010000295 DASUVE010000000
VERSION     DASUVE010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898227"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..1500)
                     /locus_tag="VF036_06350"
     CDS             complement(259..1500)
                     /locus_tag="VF036_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014765224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6264884.1"
                     /translation="MKKLSRWLVAFSTLALIAAGCGGDEGGDGDGAAVPTDPDEPRVT
                     EVGDGEGEVSIISWAGYIERGDTDPAYDWVTQFEEDTGCAVTNKTANTSDEMVALMQE
                     GGGQYDLVTASGDASLRLVESGTVQPVNLDLIESYDTVDPNLADAPWHTVDTDGDGTA
                     EHYGVPYQWGYNVLMYNDAVFKGEAPTSWSVVFEETTLPDGKSNKGRIQAYDGAIYIA
                     DAAVYLRAHNPELGITDPYALNRDQFDAALNLLRQQRELVGRYWHDAFVQIDDFGNGE
                     AVASSSWPFQRNVLAAENPDFKSSTPEEGVTGWADTTMMHTDAPHPNCAYMWMEWSLN
                     EKLQGDLAAWFGTVPANLAACEGNELLTDEGCETNGLGDFDQIEFWRTPQADCFDDDE
                     ATECVPYLEWVTQYVAVIGGR"
     gene            complement(1497..2615)
                     /locus_tag="VF036_06355"
     CDS             complement(1497..2615)
                     /locus_tag="VF036_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6264885.1"
                     /translation="MAEAARRDEARAETAGAAAVALEDVVKRFGDVVAVDGVSVDVRP
                     GEFFSLLGPSGSGKTTCLRMIAGFEHPTSGRILLDGRDVSDLPPFERDVNTVFQDYAL
                     FPHMTVAQNVGYGLMVRKLPASERASRVGDALEMVRLGHLGDRKPGQLSGGQRQRVAL
                     ARSLVMRPKVLLLDEPLGALDLKLRQAMQIELKEIQQEVSLTFLYVTHDQEEALTMSD
                     RLAVFNQGSIEQVGSPAEVYERPATAFVAGFIGVSNVLDGEVAARITGSPQAFTVRPE
                     KIAFVDADAEVDGSHCTATGHVREVVYLGAITRYIVELDEGGELVVMEQNLATSSMEA
                     LQVRGKPVRLRWERSNNRPVDTTGGNEGSATDPEEGVA"
BASE COUNT          392 a          977 c          850 g          464 t
ORIGIN      
        1 aactcctcca cgctccagaa cccgtagacg agcatcgaac cgagcgaccc gagatagatc
       61 acgccgagcc acaggagtcc tgggaggagc aggagggcga gcttggacca cggatgtcgg
      121 tggaagacgc cgctgacgcg agcgccgggt cctcgtcgga gtgccgtcac ctgcatgcgc
      181 gtacggatgg acggggcccg cgtgccgcgg gccccgtccg attcatcctc gtgtcgtccg
      241 ttgcgcctcg gacgaggctc aacggccgcc gatcacggcg acgtactgcg tcacccattc
      301 gaggtagggc acgcactcgg tcgcctcgtc gtcgtcgaag cagtcggcct gcggcgtccg
      361 ccagaactcg atctggtcga agtcacccag tccgttcgtc tcgcacccct cgtcggtcag
      421 cagctcgttc ccctcgcagg ccgcgaggtt cgctggcacc gtgccgaacc acgcggcgag
      481 gtcgccctgg agcttctcgt tcagcgacca ctccatccac atgtacgcgc agttcgggtg
      541 cggggcgtcc gtgtgcatca tcgtggtgtc agcccacccg gtgacgccct cctccggggt
      601 ggagctcttg aagtcggggt tctccgccgc cagcacgttc cgttggaacg gccaggacga
      661 gctggcaacc gcctctccgt tgccgaagtc gtcgatctgg acgaacgcgt cgtgccagta
      721 ccgaccgacc agctcccgct gctgccgcag caggttcagc gccgcatcga actggtcgcg
      781 gttcagcgcg tatggatcgg tgatccccag ctccgggttg tgggcccgca ggtagacggc
      841 ggcgtcggcg atatagatcg ccccgtcgta cgcttggatg cggcccttgt tcgacttccc
      901 gtccggcagc gttgtctcct cgaagacgac gctccagctc gtcggcgcct cgcccttgaa
      961 gaccgcgtcg ttgtacatca gcacgttgta tccccactgg tagggcacgc cgtagtgctc
     1021 ggccgtcccg tcgccgtcgg tgtcgacggt gtgccacggg gcgtccgcga ggttgggatc
     1081 gacggtgtcg tagctctcga tcaggtcgag gttgacgggc tggaccgtgc cactttccac
     1141 gaggcggagg ctcgcgtcgc cggacgccgt cacgaggtcg tactgaccgc cgccctcctg
     1201 catcagcgcg accatctcgt ccgaggtgtt ggcggtcttg ttcgtcaccg cgcagcccgt
     1261 gtcctcctcg aactgcgtga cccagtcgta cgccgggtcc gtgtcgccac gctcgatgta
     1321 gccggcccac gagatgatgc tcacttcgcc ctcgccgtca ccgacctcgg tgaccctcgg
     1381 ctcgtccggg tcggtcggga cggcggcgcc gtccccgtct cccccctcgt cgcctccgca
     1441 gccagccgcg atcaacgcga gcgtggagaa ggcaaccagc catcggctga gcttcttcat
     1501 gcgaccccct cttccgggtc cgtggcggac ccctcgttcc ctcccgtcgt gtctacgggc
     1561 cggttgttgc tgcgctccca ccggaggcgc acgggcttgc cgcgcacctg cagcgcctcc
     1621 atcgagctcg tggcgaggtt ctgctccatc acgaccagct ctcccccctc gtcgagctcg
     1681 acgatgtacc gcgtgatcgc acccaggtat acgacttcac ggacgtgccc ggttgccgtg
     1741 cagtgcgaac cgtccacctc ggcatccgca tcgacgaagg cgatcttctc cggccgcacc
     1801 gtgaacgcct gtgggctccc cgtgatccgg gcggccacct caccgtccag cacgttcgag
     1861 acgccgatga agccagccac gaacgccgtg gcgggccgct cgtacacctc ggccggcgat
     1921 ccgacctgct cgatcgaccc ttggttgaag acggcgagcc ggtcgctcat cgtcagcgcc
     1981 tcctcctggt cgtgcgtcac gtagaggaag gtaaggctca cctcctgctg gatctccttc
     2041 agctcgatct gcatcgcctg tcggagcttg aggtccagcg cgcccaacgg ctcatccagt
     2101 aggagcacct tggggcgcat cacgagcgag cgggcgagcg ccacgcgctg ccgttggccg
     2161 cccgacaact gtcccggctt gcggtcgccg agatgtccga ggcggaccat ctcgagggca
     2221 tcgccgacgc gactcgcccg ctcgctcgcg gggagcttac ggaccatcaa cccgtagccc
     2281 acgttctggg ccacggtcat gtgcgggaag agggcgtagt cctggaacac cgtgttcacg
     2341 tcgcgttcga acggcggcag atcggagacg tcccgcccgt cgaggaggat ccgacccgat
     2401 gtggggtgct cgaacccggc gatcatccgc aggcaggtgg tcttgccgga tccggagggc
     2461 ccgagcaagg agaagaactc tcccgggcgc acgtccacgc tgaccccatc gacggcgacc
     2521 acgtccccga agcgcttcac cacgtcctcg agcgcgaccg ccgcggcgcc ggccgtctcg
     2581 gcgcgcgcct catccctccg agcagcttcg gccactcgcc gcctccccct ctctcgcccg
     2641 cagcgtggcg aacccggcaa tcttgaccgc gaagaccgag gcg
//
LOCUS       DASUVE010000296         3458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_145260, whole genome shotgun sequence.
ACCESSION   DASUVE010000296 DASUVE010000000
VERSION     DASUVE010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145260"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..789)
                     /locus_tag="VF036_06360"
     CDS             complement(64..789)
                     /locus_tag="VF036_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6264886.1"
                     /translation="MDRQEYWRQRYREIRPGWTPTTAIYARLVDELVHHDTRVLDIGC
                     GHADLLGSSLARTARVVGIDVDERTLAAKEVIRYRVVADAERLPFRPGSFDLVLMAWV
                     LEHVAHPVSAFREFRRVLGAGGRIVFVTPNALNYNAWLIRMVPNAFHAFFTSRLYGRP
                     AEDTYPTRYRLNSVRRLQSTLPGLGLEPERIVLNGDPTYIAIDELFFRVGVLLERLYD
                     LRPLRNARVHIVGVYRTPRSSDA"
     gene            816..2135
                     /locus_tag="VF036_06365"
     CDS             816..2135
                     /locus_tag="VF036_06365"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6264887.1"
                     /translation="MTGRPLSRGRLSGIIVTVLALASGCTAAAPTIGDRTPAPSDRST
                     DPSDEPPPAPTRFPIKHVVFLIKENRSFDNLFGRFPGANGATVGMHHGAPRPLIRGID
                     RLPGDLPHCYSCAVAAWNEGAMDDFEQGLYGEWAYSRLGKEQLPNYWRWAKEYVLFDR
                     FFASAHGPSFPNHLYTIAAQSGGAVDNPLRNGVASYSWGCDLPEKAVVQVIDSEGDVE
                     FVPPCFDFLTEGDLLRRAGIPWAYYAAEEDQRGHIWSAYSSIRRYREHPERWQRHMFP
                     VDRVVQDIEAGRLPAVTWITPRFELSEHPEYSFCHGENWSTEVINAIMNSPMWEDTAI
                     FLTWDDYGGFYDHVPPPQVDGFGFGIRVPMIVLSPYARRGAVSHELGEFSSVLRFIED
                     NWALGRLTHRDRRATPMLSAFDFSQPPRPPVPLPVRDDCEGPKFPEG"
     gene            2176..3198
                     /locus_tag="VF036_06370"
     CDS             2176..3198
                     /locus_tag="VF036_06370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6264888.1"
                     /translation="MEDDRSSTVVPGSEPWEGTTRELAAALRDLVARLREEQGQPLPE
                     GEAFLQSPSSIRRRLKTLVFRTTRPATRRYDRLTADLATIAGDLAARLAEAEEVMRKA
                     RGDYERLEMELRALAPSGAATSGSAVWTEIPDGYYWAFEQRMRGAAGSIVDRLRGYED
                     LVVPLREAVETTDDRRPRWVDLGCGQGEFCALLGEWGWDVEGVDASPDAVEACRARGI
                     EVTLADVLTYLETRPDDPVRGVSAIQLIEHLPRRAWVHLFQEIHRALAPGGALLLETI
                     NGQNPEAIADYFVADVTHTWPGHPETLRLMAEHAGFDEVQVRFLHEDHRGRAQDVAIW
                     AVKASS"
     gene            3195..>3458
                     /locus_tag="VF036_06375"
     CDS             3195..>3458
                     /locus_tag="VF036_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264889.1"
                     /translation="MIDPKDEKQDAAGGPTPGERAPAAPENEDDAVATLAREVYWAGE
                     GGSGLPADARSLRERLDAFTYQMRADQRQAVPVGETFLGSDACW"
BASE COUNT          551 a         1176 c         1206 g          525 t
ORIGIN      
        1 agaggtctcg cagcctcgcc tcggatgcga ggtgatcgac gcgaacgccg aagatgtgcg
       61 acatcaggcg tcgctcgacc gaggcgtccg atagacaccg acgatgtgca cccgcgcgtt
      121 gcggaggggg cgcaggtcgt agaggcgttc gagcagcacc ccgacgcgga agaacagttc
      181 atcgatcgcg atgtaggtgg gatctccgtt caggacgatg cgctctggtt cgagcccgag
      241 tcccggcaac gtcgattgga gcctccgcac cgagttgagt cggtaccggg tgggataggt
      301 gtcctccgcg gggcgcccgt acaaccgcga cgtgaagaag gcgtggaacg cgttgggcac
      361 catccggatc agccaggcgt tgtagttcaa cgcgttcggc gtcacgaaca cgatccgccc
      421 gccggcaccg aggacgcgtc ggaactcgcg gaacgccgag accgggtggg cgacgtgttc
      481 cagcacccat gccatcagga cgagatcgaa ggagccgggc cggaacggga gtcgttcggc
      541 atccgcgacg acccggtagc ggatcacctc cttcgctgcg agcgttcgct cgtcgacatc
      601 gatgccgacg actcgcgcgg tccgggccag cgacgatccg aggaggtccg catgaccgca
      661 accgatgtcg aggacccgcg tgtcgtggtg aacgagttcg tccacgaggc gtgcgtagat
      721 cgccgtcgtg ggagtccacc cggggcggat ctcgcggtac cgctgcctcc agtactcctg
      781 gcgatccatc ccgcgctcac cgtaccctgt ggcggatgac cggacgacct ctgtcccgcg
      841 gacgtctgtc gggcatcatc gtcacggtac tcgccctcgc gagcgggtgt accgccgccg
      901 ccccgacgat cggggaccgg acgccggctc cctcggatcg atcgaccgac cccagcgacg
      961 agccgccgcc ggccccgacg aggttcccga tcaagcacgt ggtcttcctg atcaaggaga
     1021 accgctcgtt cgacaacctc ttcggacggt tcccgggagc gaacggagcg acggtcggga
     1081 tgcatcacgg cgctcctcgg ccactgatcc gggggatcga ccgactcccg ggagacctgc
     1141 cgcattgcta ctcgtgcgcg gtcgccgcat ggaacgaagg cgcgatggac gacttcgaac
     1201 aggggttgta cggcgaatgg gcctacagcc ggctcggcaa ggagcagctt cccaactact
     1261 ggcggtgggc gaaggagtac gtcctgttcg atcggttctt cgcgagcgcg cacgggccgt
     1321 cgttcccgaa ccacctgtac acgatcgcgg cgcagtcggg tggagccgtc gacaatccgc
     1381 tccgcaacgg cgtcgcctcg tacagctggg ggtgcgacct tcccgagaag gccgtcgtcc
     1441 aggtcatcga ctccgaaggc gacgtcgagt tcgtccctcc gtgcttcgac ttcctcaccg
     1501 agggcgacct gctgaggcgg gcggggatcc cgtgggcgta ctacgccgcc gaggaggacc
     1561 agcgcggcca catctggtcg gcctactctt ccatccgccg ctaccgcgag cacccggagc
     1621 gatggcagcg gcacatgttc cccgtcgatc gtgtcgtcca ggacatcgag gccgggcgcc
     1681 tgccggccgt cacctggatc acgccacggt tcgagctctc cgaacacccc gagtacagct
     1741 tctgccacgg ggagaactgg tccaccgagg tgatcaacgc gatcatgaac agtccgatgt
     1801 gggaggacac ggcgatcttc ctgacctggg acgactacgg ggggttctac gaccacgtgc
     1861 cgccgcccca ggtggacggc ttcggcttcg ggatccgcgt cccgatgatc gtgctcagcc
     1921 cgtacgcccg gcggggggcg gtcagccacg agctcgggga gttctcgagc gtcctgcggt
     1981 tcatcgagga caactgggca ctggggaggc tcacccatcg agaccggcgt gccacgccga
     2041 tgctgtccgc gttcgacttc tcgcagccgc cgcgaccgcc ggtcccgttg ccggtacgcg
     2101 atgactgcga gggcccgaag ttccccgagg gataaggccc gagcccaacc gcgctccggt
     2161 gtagcctgtc gcgcgatgga ggacgatcgc tcttccacgg tcgtgccggg ctcggaaccc
     2221 tgggaaggaa ccacccggga gctggctgct gcgctccgcg acctcgtcgc gcgcctgcgc
     2281 gaggaacagg gtcagccgct cccggagggg gaggcgttcc tccagtcacc ctcctcgatc
     2341 cggcgccgtc tcaagacgct cgtgttccgc acgacccggc ccgcgacacg acgatacgac
     2401 cggctcaccg ccgacctggc gacgatcgcc ggcgacctcg ccgcccggct cgccgaagcg
     2461 gaggaggtca tgcggaaggc gcgaggcgac tacgagcggc tggagatgga gctccgtgcg
     2521 ctcgcaccct ccggagcggc gacctcgggg agcgccgtct ggacggagat cccggacggc
     2581 tactactggg cattcgagca acgcatgcgc ggcgctgcgg gatcgatcgt ggatcgtctc
     2641 cgcggctacg aggacctcgt cgtcccgctc cgcgaggccg tcgagacgac cgacgaccgc
     2701 cgtccccgct gggtcgatct cggctgcggt cagggcgagt tctgcgcctt gctcggtgag
     2761 tggggctggg acgtggaggg cgtcgacgcg tcgccggacg ccgtcgaggc gtgccgggcg
     2821 cgcgggatcg aggtaacgct cgccgacgtc ctcacctatc tggagacgcg gccggacgac
     2881 ccggtccgcg gtgtcagcgc gatccagctg atcgagcatc tcccgaggcg ggcctgggtc
     2941 cacctcttcc aggagatcca ccgtgcgctc gcacccggcg gggcactcct cctcgagacg
     3001 atcaacggtc agaatcccga ggcgatcgcc gactacttcg tcgcggacgt tacccatacg
     3061 tggccggggc atccggagac gctgcggctg atggccgagc acgcgggctt cgacgaggtc
     3121 caggtgcggt tcctccacga ggatcatcgc gggcgagcgc aggacgtggc gatctgggcg
     3181 gtcaaggcgt cctcgtgatc gaccccaagg acgagaagca ggacgcggcc ggtggcccga
     3241 cacccggcga gcgggctccg gccgctcccg agaacgagga cgacgcggtc gcgaccctcg
     3301 cccgggaggt ttactgggcg ggggaggggg gctcggggct tcccgcggac gctcgctccc
     3361 tccgcgaacg gctggatgcg ttcacgtacc agatgcgcgc ggaccagcgg caggccgttc
     3421 ccgtcggcga gacgttcctc ggctcggatg cctgctgg
//
LOCUS       DASUVE010000297         4744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1087236, whole genome shotgun sequence.
ACCESSION   DASUVE010000297 DASUVE010000000
VERSION     DASUVE010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087236"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VF036_06380"
     CDS             <1..190
                     /locus_tag="VF036_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264890.1"
                     /translation="IDWVQIPAKNAGPRGSKGVGEPPQVPTAAAIANAVAKVLGRRVR
                     QLPMTPERVWAASAEVAS"
     gene            187..1098
                     /locus_tag="VF036_06385"
     CDS             187..1098
                     /locus_tag="VF036_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX6264891.1"
                     /translation="MTRTFTTATTVEGAIEAIGSGARPIAGGTDLVVGARQGKAPLPE
                     AIVAIDRIAALEGIVEDDDGLRLGALVTHEEIVASDVVRARFTALADASAIVGSHATR
                     AHGTIGGNVMNASPAMDTGGPLLCFEASARLRSASGEREVPLDDLWTGPGTTVVDPRE
                     LLVGVDVPAPIPATGSAYVRLEYRRQMEIAVVGVTAVVRLDGGSIADARVAITALAPT
                     IRRVPDAEAALIGSDGAAEALAAAARAVAAGSSPITDVRGSAGYRSAMAEVIAGRAIS
                     AALVRAQGEDVPIPASAATFAAPRGAA"
     gene            1098..1565
                     /locus_tag="VF036_06390"
     CDS             1098..1565
                     /locus_tag="VF036_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6264892.1"
                     /translation="MSVPATLHVNGVVYEVELDPHMSLLDAARDVIGLTGSKEGCDDS
                     ECGACMMLLDGKPVNSCSYLALQADGRDVTTVEGLAQGDELAPLQRALLEHGGVQCGF
                     CTPGMLISATALLREHPDPSEDDVRIALSGNLCRCTGYDGIVRAVRSAARSSG"
     gene            1660..2433
                     /locus_tag="VF036_06395"
     CDS             1660..2433
                     /locus_tag="VF036_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX6264893.1"
                     /translation="MRLRITIVTLVAALATGACSVAEGGDDLAPAVSADPTTTAVQSG
                     SAPAGTGTVDPRRGGLELGFGEFAITLEAGEIRPGPVTFVIRNGGQLVHGFEMEIEER
                     GDSSGHGSGHDGLKIEGPAFGPGETIRIATELAPGVYEIECFVAEHDEMGMRATLVVR
                     PRAPLLRVDPGPAEGEIEIADFAFAPTPLEVPVGTPVTWTNLDPTAHTVSASDGSFDS
                     GTLGSDARFETTFERPGTFAYLCQIHPTMRGVVRVEEPG"
     gene            2514..2873
                     /locus_tag="VF036_06400"
     CDS             2514..2873
                     /locus_tag="VF036_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6264894.1"
                     /translation="MKTMKLHTSIAAAGLILLASCGGGGDDGGAPDGGEASSTVTVRD
                     NAFAPADPVIGAGDVELVNEGESPHTFTIEGEDVDVEVDAGQTTTVTVDLPAGTYTLF
                     CSFHRGQGMEGTLTVEA"
     gene            2946..4151
                     /locus_tag="VF036_06405"
     CDS             2946..4151
                     /locus_tag="VF036_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6264895.1"
                     /translation="MGRTVFHGGRVFDGTGAALADADVLIEGERIVDVGPGLDGDEGI
                     DCSGKAILPGLFDTHVHLVSRYEDFDEVRVLHEPFSAPFYRVAENMRRTLACGITTVR
                     DAAGADAGMRLAVEEGSLVGPRMQISVTMLSMTGGHNDPWLPSGATGPWGQPYPGMPS
                     GVCDGIDGVAAKVREVIRAGADVIKIASSGGFLSPTDDPSQPNFSEAEVEAIVRTAAD
                     LGRWVMSHAHGAEGIKRAVRAGVRSIDHGTYLDDEGIELMRERGTWLVPTLTAGDTTD
                     EIAANPQVAEAVREKLRELGRPELDAFRRAADAGVKVAMGTDCPVAPHGTNLRELELM
                     AANGFTPEQVLVASTSSAAELMGLEGSLGTLEPGKIADVVIVDGDPFEFATLADRIEQ
                     VWKAGLRLV"
     gene            4239..4709
                     /locus_tag="VF036_06410"
     CDS             4239..4709
                     /locus_tag="VF036_06410"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX6264896.1"
                     /translation="MRSRSIRLVVVVLAGAAVLATGLLAHASSPADAGGPAVRLARLT
                     GAKEVPGPGAEGGTGVATVIVHPSQGRLCFSIAVQGIELPAAAAHVHVGGPDVAGPVV
                     VGLTPPDAAGSSAGCVTADPALLRAIKRTPWAYYVNVHTTSFPDGAVRGQLRRP"
BASE COUNT          686 a         1581 c         1795 g          682 t
ORIGIN      
        1 gatcgactgg gtgcagatcc cagcgaagaa cgcgggcccg cgtggctcca agggcgtcgg
       61 tgagcccccg caggtcccga ccgccgccgc gatcgcgaac gcggtcgcca aggtgctcgg
      121 ccggcgcgtt cggcagctcc cgatgacgcc cgagcgggtc tgggccgcgt ccgccgaggt
      181 cgcgtcgtga cgaggacgtt caccacggcg accacggtgg agggcgcgat cgaggccatc
      241 ggctcgggag cgcggccgat cgccgggggc accgacttgg tggtcggggc ccggcagggg
      301 aaggcgccgc tcccggaagc gatcgtcgcc atcgatcgga tcgccgcgct cgaggggatc
      361 gtggaggatg acgacggact gcgtctgggc gcgctcgtca cgcatgagga gatcgtcgcg
      421 agcgacgtgg tccgtgcgcg gttcaccgcc ctcgccgacg cgtccgcgat cgtcggctcc
      481 cacgcgaccc gcgcgcacgg cacgatcggc ggcaacgtga tgaacgcctc gcccgcgatg
      541 gacaccggcg ggccgctcct gtgcttcgag gcatccgctc gtctccggtc ggcgtcgggg
      601 gagcgggagg tgccgctcga cgacctgtgg accggaccgg gaacgaccgt ggtcgatcca
      661 cgcgagttgc tcgtgggggt cgacgttccg gcgccgatcc cggcgacagg ctccgcgtac
      721 gtccgcctcg agtaccgccg tcagatggag atcgccgtgg tgggcgtgac ggccgtcgtg
      781 cggctcgacg gcgggtcgat cgcggacgcg cgtgtcgcga tcacggcgct ggctcccacg
      841 atccgccgcg tcccggacgc ggaggcggcg ctcatcggtt ccgacggcgc cgccgaggcg
      901 ctcgcggccg ccgcccgagc ggtggctgcc ggctcctccc cgatcacgga cgtccgtggg
      961 tccgccggat accgctcggc gatggcggag gtgatcgccg gccgggcgat ctcggccgcg
     1021 ctcgtccgcg cccagggcga ggacgtcccg atccccgcga gcgccgccac gttcgcagct
     1081 ccccgagggg cggcctgatg agcgtcccgg caacgctgca cgtcaacggg gtcgtgtacg
     1141 aggtggagct ggatcctcat atgagtctgc tcgacgccgc ccgcgacgtg atcgggctca
     1201 cgggctccaa ggagggatgc gacgactccg agtgcggggc gtgcatgatg ctcctcgacg
     1261 gcaagccggt gaactcctgc agctacctgg cgctccaggc cgacggccgg gacgtcacga
     1321 cggtcgaggg tctggcgcag ggcgacgagc tggctcccct ccaacgcgcg ctcctcgagc
     1381 acgggggcgt gcaatgtggc ttctgtaccc cggggatgct gatctcggcc accgctctgc
     1441 tgcgcgagca cccggatccg tccgaggacg acgtgcggat cgcgctctcc gggaacctct
     1501 gccggtgcac cggatacgac gggatcgtcc gcgcggtccg gagtgcggcc cggtcgtcgg
     1561 gctgacgccg actcgggtca acgaagccca ccccgttctt tccttcccct ttgccgtcgg
     1621 ttggcgcgac cttggagcgg catcgagcag cctgcatgca tgcgcttgag gatcacgatc
     1681 gtcacgctcg tggccgccct cgccaccggc gcctgctcgg tcgccgaggg gggcgacgat
     1741 ttggcgcccg cggtctccgc cgatcccacc acgacggcgg ttcagagcgg gtcggcacct
     1801 gccggcaccg gcacggtgga cccccggcgt ggcggtctcg agctcgggtt cggcgagttc
     1861 gcgatcaccc tggaggccgg cgagatacgg ccggggccgg tcacgttcgt gatccgcaac
     1921 ggtgggcagc tcgtccacgg tttcgagatg gagatcgaag agcggggaga cagcagcggg
     1981 cacggctcgg gccatgacgg gctgaagatc gaggggccgg cgttcggacc cggggagacg
     2041 atccggatcg cgacggagct cgcccccggc gtctacgaga tcgagtgctt cgtcgccgag
     2101 cacgatgaga tggggatgcg cgcgacgttg gtcgtcaggc cgcgtgcgcc gctcctgcgc
     2161 gtcgatccgg gaccggccga gggtgagatc gagatcgccg acttcgcgtt cgcaccgacg
     2221 ccgctcgagg taccggtggg gaccccggtg acgtggacga acctcgaccc caccgcccac
     2281 accgtgagcg cgagcgacgg ctcgttcgac tcgggcaccc tcgggtcgga cgcgcgattc
     2341 gagaccacat tcgagcgacc cgggacgttc gcctacctct gccagatcca tccgacgatg
     2401 cgcggcgtcg tccgcgtgga ggaaccgggg tgagtcgtgt ccgaagcggc tccggtcagg
     2461 ccagatgggt gccacatggc cagatcgatc gtcacacgga aaggagaaca ccgatgaaga
     2521 ccatgaaact gcacacgtcg atcgcggcgg ccggcttgat cttgctggcg tcgtgcgggg
     2581 gcggcggtga cgacggaggc gcgcccgatg gtggtgaggc atcgtccacg gtcacggtcc
     2641 gcgacaacgc attcgcgccg gctgatccgg tgatcggggc aggtgacgtc gagttggtga
     2701 acgaagggga gtcgccccac acgttcacga tcgagggcga ggacgtcgac gtggaggtcg
     2761 acgcggggca gacgaccacc gtgaccgtgg atctcccggc ggggacgtac accctcttct
     2821 gcagcttcca tcgaggacag gggatggagg gcacgctcac cgtcgaagcg tgagcgggag
     2881 ccctcctgga gggccggccc gcgcccgtat gctcgcgggc gcgggtcgga tcgaggaggc
     2941 atcgcgtggg cagaaccgtg tttcacgggg gacgggtgtt cgacggcacc ggggccgcgc
     3001 tcgccgatgc cgacgtcctg atcgagggcg aacggatcgt cgacgtgggc cccggtctcg
     3061 acggcgacga ggggatcgac tgctcgggca aggcgatcct gcccggccta ttcgacaccc
     3121 acgtgcacct ggtctcgcgt tacgaggact tcgacgaggt ccgcgtgctg cacgagccgt
     3181 tcagcgcgcc cttctaccgc gtcgcggaga acatgcgtcg aaccctcgcg tgcgggatca
     3241 cgacggtgcg tgacgctgcc ggagcggatg ccgggatgcg gctcgccgta gaggagggct
     3301 cgctcgtcgg cccgcggatg cagatcagcg tcacgatgct ctcgatgacg ggagggcaca
     3361 acgacccctg gctcccgagc ggcgcgaccg gcccctgggg acagccctac ccggggatgc
     3421 cgagcggtgt ctgcgacggc atcgacggcg tggccgcgaa ggtgcgggag gtgatccgtg
     3481 ccggcgccga cgtgatcaag atcgcttcgt ccggaggctt cctctcaccc accgacgatc
     3541 catcgcagcc gaacttctcg gaggccgagg tcgaggcgat cgtgcgcacc gccgccgacc
     3601 tcgggcggtg ggtgatgtcg catgcgcacg gagccgaggg gatcaagcgc gccgtccgcg
     3661 ccggcgtgcg ctcgatcgat cacggcacct acctcgacga cgaggggatc gagctgatgc
     3721 gggagcgcgg cacctggctc gtccccacgc tgaccgcggg ggacacgacc gacgagatcg
     3781 ccgcgaaccc tcaggtggcc gaggcggtcc gggagaagct ccgcgagctc gggagaccgg
     3841 agctggacgc gttccgacgg gccgccgatg ccggcgtgaa ggtggcgatg ggcaccgact
     3901 gtccggtcgc cccgcacggg acgaaccttc gcgagctgga gctgatggcc gccaacggtt
     3961 tcacgcccga gcaggtgctc gtcgcctcga cgtcgagcgc cgcggagctg atgggcctgg
     4021 aggggtccct cgggaccctc gagcccggca agatcgccga tgtggtgatc gtcgacggcg
     4081 acccgttcga gttcgcgacg ctcgcggatc gcatcgagca ggtctggaag gcgggactgc
     4141 gtctcgtgtg atcggccgcc tgttgccttg acgggcgagg tgacacttcg aaggatggag
     4201 cggaggtcga gtccccgaga ggggaggagg gaggcgtcat gcgttccagg tctatccgcc
     4261 tcgtcgtggt cgttctcgcc ggagcggcgg tgctcgcgac ggggctcctg gcccacgcga
     4321 gctcgccggc tgacgccggc gggccggccg tcaggctcgc tcggttgacc ggagccaagg
     4381 aggtgccggg gccgggcgcg gaaggcggga ccggcgtggc cacggtgatc gtgcatccga
     4441 gccagggcag gctgtgcttc tcgatcgcgg tgcaggggat cgagttgcct gcggccgcgg
     4501 cccacgtgca cgtggggggt cccgatgtcg ccggacctgt cgtggtggga ctgaccccac
     4561 cggacgcagc gggcagctcg gcaggctgcg tcaccgccga tccggcactg ctgagggcga
     4621 tcaagcggac accgtgggcg tactacgtga acgtccacac gacctcgttc ccggatggag
     4681 ccgtcagagg tcagctccgt aggccctgac gcggtgacgg agagccggcc gcggagcggc
     4741 cggc
//
LOCUS       DASUVE010000298         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_712484, whole genome shotgun sequence.
ACCESSION   DASUVE010000298 DASUVE010000000
VERSION     DASUVE010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712484"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VF036_06415"
     CDS             <1..259
                     /locus_tag="VF036_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264897.1"
                     /translation="DVRIGDVARGAIVAQSAFGTLEVGVRDGAAVWLDLDTKFGTVES
                     ELDVSSSPGADDQVVEVHAHTSYGDISVHRAMATADGRDGS"
     gene            256..1212
                     /locus_tag="VF036_06420"
     CDS             256..1212
                     /locus_tag="VF036_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005456544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6264898.1"
                     /translation="MKSNGSTIVEVTGLRKGFGDTTALDGVDLHVTAGTTFALLGPNG
                     AGKTTTVHILSTLLAADEGVVRVAGYDAANEPDAVRRVIGVTGQFSAVDTLLTGEENL
                     TLMADLFHLDRAEGRRRVAGLLERFDLVDAARRPAVTYSGGMRRRLDLAMTLIGEPRI
                     IFLDEPTTGLDPRSRRTVWRIVRELVDEGVTIFLTSHYLEEADELADRVAVLDRGRLI
                     AEGTPDELKRLIPGGFVRLELADSERLGSAAKAFPDGTRDEDALTLQIPSDGGAGSLR
                     DVLDRLDAASIDVERVSVHTPDLDEVFLTLTGGPGEDGKELG"
     gene            1209..2000
                     /locus_tag="VF036_06425"
     CDS             1209..2000
                     /locus_tag="VF036_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013415158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6264899.1"
                     /translation="MSTNAYVIRDSATMLRRNFRHVIRYPSVSLLVVTVPVILLLLFV
                     YVFGGTLGAGLGPVADGRAAYLDYVVPGILLIAVAGGSQGTAISVAMDMTEGIIARFR
                     TMGIARSSVLLGHVLGSTIQTVAGLAVVVGIAVLIGFSPNATLVEWIATVGVLAMITF
                     AITWLSVALGLFPKTVEAASNLPMPLLLLPFMSSGFVPTESMPAGVQWFAEYQPFTPF
                     IETVRGLLMGTPIDNSAWLTLGWCVAIAFVGWRWGMKLYERPPRP"
BASE COUNT          332 a          773 c          801 g          370 t
ORIGIN      
        1 ggacgtccgc atcggcgacg tcgcgcgcgg cgcgatcgtt gcgcagagcg cgttcggcac
       61 cctcgaggtc ggtgtccggg acggcgcggc cgtctggctc gacctcgaca ccaagttcgg
      121 cacggtggag agcgagctgg atgtctcgtc cagccccggc gcggacgacc aggtggtcga
      181 ggtccacgcc cacacctcgt acggcgacat cagcgtccac cgagccatgg cgaccgccga
      241 cgggagggat gggtcatgaa gtcgaacggt tccacgatcg tggaggtgac cggcctccgg
      301 aagggcttcg gcgacaccac ggccctggac ggcgtcgacc tccacgtgac ggcgggcacg
      361 acgttcgcgc tcctcggccc gaacggtgcc ggcaagacca cgaccgtgca catcctgtcc
      421 accctgctcg ccgccgacga gggcgtcgtg cgggtcgccg gctacgacgc ggcgaacgaa
      481 ccggatgccg tgcgccgggt gatcggcgtg accgggcagt tctccgcggt ggacacgctc
      541 ctcaccggcg aagagaacct cacgctgatg gccgatctgt tccacctcga tcgtgccgag
      601 ggtcgacggc gggtcgccgg cctcctcgag cggttcgacc tggtggacgc ggcccggcga
      661 cccgccgtga cgtactcggg cggtatgcgg cgcaggctgg accttgcgat gaccctgatc
      721 ggcgagccgc ggatcatctt cctcgacgag ccgaccaccg gactcgaccc gcgcagccgt
      781 cgcaccgtct ggcggatcgt ccgtgagctg gtagacgagg gcgtcacgat cttcctcacg
      841 tcgcactacc tggaggaggc cgacgagctg gccgaccggg tcgcggtgct cgaccgcggg
      901 cggctgatcg cggagggaac ccccgacgag ctgaagcgac tgatccccgg cgggttcgtc
      961 cgcctggagc tcgcggactc cgaacggctg ggatcggccg ccaaggcgtt ccccgacgga
     1021 acgcgggacg aggacgcact caccctgcag atcccgagcg acggcggcgc cggctcgctc
     1081 cgagacgtgc tcgaccgcct ggatgcggcg tcgatcgacg tggagcgcgt gtcggtccac
     1141 acgccggacc tcgacgaggt gttcctcacc ctgaccggcg gtccgggtga ggacgggaag
     1201 gagctcggat gagcacgaac gcgtacgtga tccgtgactc ggccacgatg ctgcggcgga
     1261 acttccgtca cgtgatccgc tacccctccg tctcgctcct ggtcgtgacg gtcccggtga
     1321 tcctcttgct cctgttcgtg tacgtgttcg gcgggacgct cggcgccggg ctcggtccag
     1381 tcgcggacgg ccgcgccgcg tacctcgact acgtggtgcc cgggatcctg ctgatcgcgg
     1441 tggccggggg atcgcagggc acggcgatct cggtggcgat ggacatgacc gaggggatca
     1501 tcgctcgatt ccgcacgatg gggatcgccc gctcctccgt gctgctcggc cacgtcctcg
     1561 gcagcacgat ccaaacggtg gccggcctgg cggtcgtggt cgggatcgcc gtcctgatcg
     1621 gcttctcgcc gaacgcgacc ctcgtcgagt ggatcgcgac ggtcggcgtg ctggcgatga
     1681 tcacgttcgc gatcacgtgg ctgtcggtgg cgctcgggct gttccccaag acagtggaag
     1741 ccgcgagcaa cctcccgatg ccgttgctgc tcctgccgtt catgagcagt gggttcgttc
     1801 ccacggagtc gatgcccgcg ggggtgcagt ggttcgccga gtatcagccc ttcacgccgt
     1861 tcatcgagac cgtgcgcggg ctcctgatgg ggacgccgat cgacaacagc gcgtggctca
     1921 ccctcggctg gtgtgtggcg atcgccttcg tcggatggcg gtgggggatg aagctgtacg
     1981 agcgtccacc gcgaccctga gcggaggcga ggcggatccc ggtgaggtgt gcacgagaga
     2041 tcatggatcg catcggtggc tccccccacg acccaccgac tccgttggtg acgtcgtgat
     2101 ccgcgtcagc ggttctccga aagtcggtcg gcgatcgcgc ggaacaagga gagccatccg
     2161 tcgttctcga tcccccagtc cctccccatc cggacgatga ccgcgtccgc gtcgggcgcc
     2221 acgtagacgt actggccgaa gttccccagg gcgtagaacc gatcaggtcg ttcctc
//
LOCUS       DASUVE010000299         3970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_713112, whole genome shotgun sequence.
ACCESSION   DASUVE010000299 DASUVE010000000
VERSION     DASUVE010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713112"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      <1..66
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            93..1037
                     /locus_tag="VF036_06430"
     CDS             93..1037
                     /locus_tag="VF036_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003938314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6264900.1"
                     /translation="MVHRRIGGIAAVLFLLPGSCGESTTPNASPETPSTAAAFPVTIA
                     AANGDVTLEEPPERIVSLSPTATEMLFAIGAGDQVEAVDDQSNYPPDAPVTDLSGFEP
                     NVEAIASYEPDLVVYATEPGDLGASLEGLGIPALQQDAAASLDDVYAQIDQLGMATGN
                     PTESAELVDGMRGDIGTIVASVAPAEPPLSFYHELDDTYYSVTSSTFIGQLYALIGLV
                     NIADEAKGAGGGYPQLSAEYIIQANPDLIFLADTKCCGQSSATVAERPGWSRIDSVEG
                     GDVISLDDDIASRWGPRVVDFLRRIAAAVEGVPEANAA"
     gene            1034..2047
                     /locus_tag="VF036_06435"
     CDS             1034..2047
                     /locus_tag="VF036_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017570639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6264901.1"
                     /translation="MSRRVHWIALGVGLLLLSIVAGLLVGPVHIAPGDVLRWLGSFGN
                     PPPGMSEQQAAILGNLRLPRVIVAALVGASLSASGAAYQAVFRNPLADPYLLGAAAGA
                     GLGATIAVVFGPSTAFALTVPLAAFIGAVAGVGMAYGLGRSSLGGRSSTSLVLGGIAV
                     ASFLTAVQTFIQQQNAETLRQVYSWILGRLGTTGWDDVRIALPSVVVAVSILWLVRRL
                     LDVIEVGDLEAESLGLYVGRLRVVVVVAASLATAAAVSVAGLIGFVGIIVPHATRLLA
                     GTSYRQVLPLSVLFGASFLILVDILARTAVAPAEIPIGVITAFVGAPFFVLALRRAGR
                     RSA"
     gene            2071..2853
                     /locus_tag="VF036_06440"
     CDS             2071..2853
                     /locus_tag="VF036_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6264902.1"
                     /translation="MALEASGVGVRLGGQEILRDVAFEVPAGSWVCAIGPNGAGKTTL
                     VHTVAGLIAFDGRVDLWGRELRGYSNRERARLVALVPQKPVIPEGIRVEEYVLLGRTP
                     HLGVLGTERKADLVAVAIALEQLELGWAAGRRLDSLSGGELQRAVLARALAQEAPLLL
                     LDEPTTGLDLGHQIRVLELVDGLRRTHGLTVLSTMHDLTLAGRFADRFALLSQGRLVS
                     TGTRTDVLNAAVIAEHFGASVRVIGDADIGWAVVPISEKGEA"
     gene            2850..3419
                     /gene="cobO"
                     /locus_tag="VF036_06445"
     CDS             2850..3419
                     /gene="cobO"
                     /locus_tag="VF036_06445"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX6264903.1"
                     /translation="MTGTERPPEAPPPRARERVPSRVLVHTGDGKGKTSAAMGVVMRA
                     VARRWRVCVVQFVKDGRWRTGEETSARKLGVEWWSIGDGFTWDSDDIDRTKAISGEAW
                     RVASDVILAGDHHLVVLDEITYPMNWGWIDADQVVQVIRDRPSHVNIVATGRDAPASL
                     RAVADTVTEMTNLKHPFEQGVTAMRGIEF"
     gene            3419..3946
                     /gene="cobU"
                     /locus_tag="VF036_06450"
     CDS             3419..3946
                     /gene="cobU"
                     /locus_tag="VF036_06450"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX6264904.1"
                     /translation="MGLSVLLGGARSGKSDLSGRLATASGRPVVVIVTGEARDEEMTE
                     RIRRHRAARPSSWETVEAPRALREAVRELDGGAFAVLDCLTLWVSNLIEAGVSDDEIL
                     DEAREIATTLAERDASSVVVTNEVGLGIVPMHELARRYRDTLGRVNAAFVSEAQHAYL
                     VVAGRALSLEGVMPT"
BASE COUNT          541 a         1349 c         1439 g          641 t
ORIGIN      
        1 gcccgggaag ggtcggggag cgaggatccg ggagtcagga gacctgcccg agcgcgagag
       61 gaccgtcgcc ttcgcgtgaa gggagacgcg ccatggtcca ccgtcgcatc ggcgggatcg
      121 ccgccgtcct gttcctcctc cccggctcgt gcggggagtc cacgaccccg aacgcatcgc
      181 cggagacgcc gtcgacggcg gccgcgttcc cggtcaccat cgcggcggcg aacggagacg
      241 tgacgctcga ggaacctccg gagcgcatcg tgtccctctc cccgacggct accgagatgc
      301 tcttcgcgat cggcgccggc gatcaggtcg aggcagtcga cgaccagtcg aactacccgc
      361 ccgatgcgcc ggtcaccgac ctctcggggt tcgagccgaa cgtggaggcg atcgcctcgt
      421 acgagcccga cttggtcgtc tacgcgacgg agccgggcga cctgggtgcg tccctcgaag
      481 gcctcggcat cccggcgctg cagcaggatg cggcagcctc cctcgacgac gtctacgcgc
      541 agatcgatca gctcggcatg gcgacgggca acccgacgga gtcggccgaa ctcgtggacg
      601 ggatgcgcgg ggacatcggc accatcgtcg cctcggtcgc gccggccgag ccaccgctga
      661 gcttctacca cgagctcgac gacacgtatt actccgtcac gtcgtcgacg ttcatcggcc
      721 agctgtacgc gctcatcggc cttgtcaaca tcgcggacga agcgaaaggg gcggggggcg
      781 gctacccgca actctccgcc gaatacatca tccaggcgaa ccccgacctg atcttcctcg
      841 ccgacacgaa gtgctgcggc caatcttcgg ccacggtggc ggagcgtccc ggctggagcc
      901 ggatcgactc ggtggaggga ggcgatgtga tctcgttgga cgacgacatc gcctcacgtt
      961 ggggaccgcg ggtcgtcgac ttcctgcggc ggatcgcggc cgctgtcgag ggcgtgccgg
     1021 aggcgaacgc ggcgtgagcc gacgcgtcca ctggatcgcg ttgggcgtcg ggctgctcct
     1081 cctttcgatc gtcgccgggt tgctcgtggg gccggtgcac atcgccccag gcgatgtgct
     1141 tcggtggctg gggtccttcg gcaacccgcc gcccggcatg tccgagcagc aggccgcgat
     1201 cctcgggaac ctgcgactcc cgcgcgtgat cgtggccgcg ctcgtcggcg catcgctgtc
     1261 cgcatcggga gcggcctatc aggcggtgtt ccggaatccg ctggccgatc cgtacctgct
     1321 gggagccgcc gccggtgccg ggctcggcgc cacgatcgcg gtcgtcttcg gtccgtcgac
     1381 ggccttcgcc ctcaccgtcc ccttggcggc gttcatcggc gcggtggcgg gggtcggcat
     1441 ggcctacggt ctcgggcgct ccagcctcgg cggtcgatca tcgacgagtc tggtcctcgg
     1501 cggcatcgcc gtggcatcgt tcctcaccgc ggtgcaaacg ttcatccagc aacagaacgc
     1561 cgagaccctc cgtcaggtct attcgtggat cctcgggcgc ctcggcacga ccgggtggga
     1621 cgacgtgagg atcgcgctcc catccgtcgt ggtggccgtc tcgatcctct ggctcgttcg
     1681 ccgcctcctg gacgtgatcg aggtggggga cctcgaggcc gagagcctcg gtctgtacgt
     1741 gggacggctc cgggtcgtcg tggtggtggc tgcgtcgctc gcgaccgcgg ccgccgtctc
     1801 cgttgcaggg ctgatcgggt tcgtcgggat catcgtcccg cacgcgaccc ggctgctcgc
     1861 cggcacctcg tatcggcagg tgttgccgct ctcggtcctc ttcggggcct cgttcctgat
     1921 cctcgtcgac atcctggcgc ggaccgcggt ggctcccgcg gagatcccga tcggggtgat
     1981 cacggcgttc gtcggtgctc cgttcttcgt gctcgcgctc cggcgagcgg ggcggcgatc
     2041 ggcgtgaagc tccgaccgga ggaggacccc gtggcgctcg aggcatcggg cgtgggcgtt
     2101 cgtctcggcg gacaggagat cctccgcgac gtcgcgttcg aggtaccggc gggctcgtgg
     2161 gtgtgcgcga tcggcccgaa cggcgccggg aagaccaccc tcgtgcacac ggtggccggc
     2221 ctgatcgcct tcgatggccg tgtcgatctc tggggccgcg agctgcgggg ctactccaat
     2281 cgcgagcgag cacgcctcgt ggccctcgtg ccgcagaagc ccgtgatccc cgaggggatc
     2341 cgggtggagg agtacgtact gctcgggagg acgccgcacc tcggcgtctt gggaacggag
     2401 cggaaggcgg atctggtggc ggtggcgatc gcgctcgagc aactcgagct cggatgggcg
     2461 gccgggcgac gcctcgactc gctgtccggc ggtgagttgc agcgggcggt gctcgctcga
     2521 gcgctcgccc aggaggcgcc gctgctgctc ctcgacgagc cgacgaccgg gctcgatctc
     2581 ggtcatcaga tccgcgtcct cgagctggtc gacgggctcc gacgcacgca cggcctgacg
     2641 gtcctctcga cgatgcacga cctcacgctc gcgggacgct tcgccgatcg cttcgccctg
     2701 ctctcccagg gccgtctggt ctcgacgggg actcgcacgg acgtgctcaa cgctgcggtg
     2761 atcgccgagc atttcggcgc gagcgtccgg gtgatcgggg acgcggacat cggctgggcc
     2821 gtcgtcccga tctccgagaa gggcgaagcg tgacgggcac ggagcggccg ccggaggcgc
     2881 cgccgcctcg tgcccgcgaa cgtgtgccct cgcgcgtgct cgtccacacg ggcgacggca
     2941 aggggaagac ctccgcggcg atgggcgtcg tgatgcgcgc cgtcgcgcgc cgatggcgcg
     3001 tctgcgtggt gcagttcgtg aaggacggtc gatggcggac cggcgaagag acctccgccc
     3061 ggaagctcgg cgtcgagtgg tggtcgatcg gcgacgggtt cacgtgggac tccgacgaca
     3121 tcgaccgcac gaaggcgatc tcgggcgagg cgtggcgggt cgccagcgac gtgatcctcg
     3181 ccggcgacca tcacctcgtc gtgctcgacg agatcaccta tcccatgaac tggggatgga
     3241 tcgacgcgga ccaggtggtc caggtgatcc gcgaccggcc ctcgcacgtg aacatcgtgg
     3301 ccaccggtcg cgacgcgccg gcgtcgctcc gcgccgtggc ggacaccgtg accgagatga
     3361 cgaacctcaa gcatccgttc gagcagggcg tcaccgcgat gcgcgggatc gagttctgat
     3421 gggcctctcc gtgctcctcg gcggtgcacg cagcgggaag tcggatctct cggggaggct
     3481 cgcgacggcc agcgggcggc ccgtcgtggt gatcgtgacc ggagaggccc gggacgaaga
     3541 gatgacggag cggatccgac gacaccgagc ggcgcggccc tcgtcgtggg agaccgtcga
     3601 ggcccctcgc gcgcttcgcg aggccgtgcg cgagcttgat ggcggcgcct tcgccgtact
     3661 cgactgcttg accctctggg tctcgaacct gatcgaagcc ggggtgagcg acgacgagat
     3721 cctcgacgag gctcgcgaga tcgcgacgac gctcgcggag cgcgatgctt cgtccgtggt
     3781 ggtcaccaac gaggtgggcc tcgggatcgt tccgatgcac gagctcgccc gccgctaccg
     3841 ggacacgctg ggtcgggtga acgccgcgtt cgtcagcgaa gcccagcacg cgtacctcgt
     3901 ggtcgcaggg agggcgctct ccttggaggg agtgatgccc acgtgaccgg caccttcgac
     3961 accgtcgtgc
//
LOCUS       DASUVE010000300         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_899775, whole genome shotgun sequence.
ACCESSION   DASUVE010000300 DASUVE010000000
VERSION     DASUVE010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899775"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1731)
                     /locus_tag="VF036_06455"
     CDS             complement(<1..1731)
                     /locus_tag="VF036_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6264905.1"
                     /translation="MTTPTPLNARDFPIEDLSFQDVLGSMPGVAYVEAEGPTGMTAYM
                     SPRIEALTGYPPERFTESRAFWYSLVHPDDRERVELSDVAADAELEPYLDEYRLVVAG
                     GRVIWVRDEAVHVGASDGRAAHWIGLIVDITKEKEAEARAAEVEHRYQTLVEQLPIVT
                     YIDAPDASLENLFVSPQVNDLLGRPSGSTGEEWAQRVHPDDRERAHRETVEGVRSGDP
                     FTLEYRMVRDDGRVIWVRDSATTVRNDAGEPAYVLGFFFDITKRKEAELQLEELERRY
                     RNLVEQLPAVTYMDTADGDLTSIYVSPQVESVLGLSADAFVSDDAWAGHLHPEDRDEA
                     VRGIREAIRRAEPFTLEYRMIRPDGSLVWIRDQGTVVKDEDGHPAYVQGLYVDISEQK
                     RLESELRSEAVKFKSLAEQIPAVVYIEGEGGSGDSPVYISPKYQELFGYTPEERQARP
                     NLWLELLHPEDRERVLLAEKQASDEHPFSEDYRMVARDGRLVWVHDETVPVRDESGEL
                     LFYQGVMYDVTEQKRAERELAQALDMERKAVERLREADDMKNTFLTAVSHDLRTPLSA
                     ILGSAITLESA"
     gene            complement(1847..>2381)
                     /locus_tag="VF036_06460"
     CDS             complement(1847..>2381)
                     /locus_tag="VF036_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6264906.1"
                     /translation="DLLDTERLARGVVEPKLQEIAVGRLISRLVWDSEAMAGRQVHVD
                     TRSVSAWVDPEMISRIVENLLMNAARHTPSECQVWVKVLPEGDHVLIEVEDDGPGIPE
                     EHRESIFEPFEHGPMTSVRNPGVGIGLSIVARFAELHGGRVWVQDREGGGASFRVLIP
                     TGDPEVVPESGEPGERP"
BASE COUNT          351 a          841 c          763 g          426 t
ORIGIN      
        1 ggcactctcg agcgtgatcg cgctcccgag gatcgccgac agcggcgtcc tcaagtcgtg
       61 ggacaccgcg gtgaggaacg tgttcttcat atcgtcggcc tcgcgcagtc gctccacggc
      121 cttccgctcc atgtcgagcg cctgcgccag ctcccgctcg gcccgcttct gctcggtgac
      181 gtcgtacatc acaccttggt agaagagcag ctcgcccgat tcgtcgcgga ccggcacggt
      241 ctcgtcatgg acccagacga gtcttccgtc acgcgcgacc atgcgatagt cctcgctgaa
      301 cgggtgctcg tcgctcgcct gcttctcggc aaggagcacc cgctctcgat cctctggatg
      361 gagcagctcg agccagaggt tggggcgcgc ctgccgttcc tccggcgtgt acccgaagag
      421 ctcctgatac ttcgggctga tgtaaaccgg gctgtcgccg gaacctccct ctccctcgat
      481 gtagacgacg gccgggatct gctcggccag cgatttgaac ttcacggcct cggaccggag
      541 ctcggactcg aggcgcttct gctccgagat gtcgacgtag agcccctgga cgtacgcggg
      601 gtgaccgtcc tcgtccttga cgacggtgcc ctggtcacgg atccacacga ggctgccgtc
      661 gggccggatc atccggtatt cgagcgtgaa cggctccgct cgtcggatcg cctcgcggat
      721 ccctcgcacc gcctcgtcac gatcctcggg gtgcaggtgc ccggcccacg cgtcgtcgct
      781 gacgaatgca tctgcggaga gccccagcac gctctcgacc tgcgggctca cgtagatcga
      841 ggtcagatcg ccgtcggcag tgtccatgta cgtcacggcc gggagctgct ccactaggtt
      901 gcggtagcgc cgctccagct cctcgagctg cagttcggcc tccttccgct tcgtgatgtc
      961 gaagaagaac ccgaggacgt atgcgggttc ccctgcatcg ttgcgcacgg tggttgcgga
     1021 gtcgcgcacc cagatcaccc gtccgtcgtc cctcaccatg cggtactcga gcgtgaacgg
     1081 gtcgccggat cgaacaccct cgacggtctc gcggtgagcg cgctcgcgat cgtccggatg
     1141 gacccgttga gcccattcct cgcccgtcga gcccgagggc ctcccgagaa ggtcgttgac
     1201 ctgcgggctc acgaagaggt tctcgaggct cgcgtcgggc gcgtcgatgt acgtgacgat
     1261 cgggagctgc tcgaccaggg tctggtagcg gtgctcgacc tcggcggcgc gtgcctcggc
     1321 ttccttctcc ttggtgatat ccacgatcag gccgatccag tgcgccgctc ttccatcaga
     1381 cgcgccgacg tgcacggcct cgtcccggac ccagatcacg cgtccgcccg cgacgacgag
     1441 gcggtactca tccagatacg gttccagttc cgcgtccgca gccacgtcgg agagctccac
     1501 ccgttcgcga tcgtccggat ggacgaggga gtaccagaac gcgcgagact cggtgaagcg
     1561 ctccggcgga tagcccgtga gcgcctcgat ccgcgggctc atgtacgccg tcatccccgt
     1621 cggcccttcg gcctcgacgt aggccacgcc gggcatggac ccgagcacgt cttggaagct
     1681 caggtcctcg atcgggaaat cgcgcgcgtt cagcggcgtc ggggtcgtca tgggtcgctc
     1741 ggtatgtgtt gtgggtccgt aggaagcatc ggcgctcttc cgcccgcacg tgaatggccc
     1801 ttccgagccc ggcacgttgg gcccatccgg catcccccgc cccttctcag ggacgttcgc
     1861 ccggttcccc gctctcgggc acgacctcgg gatcgcctgt cgggatcagg acccggaagg
     1921 aggcgccgcc gccctcgcga tcctggaccc agacgcgacc cccgtggagc tcggcgaacc
     1981 tggcgacgat cgagagcccg atgcccaccc ccgggttgcg cacggaggtc atgggtccgt
     2041 gctcgaaggg ctcgaagatc gactcccgat gctcctcggg gatccccggg ccgtcgtcct
     2101 ccacctcgat caggacgtgg tcgccctcag ggagcacctt gacccagacc tgacactcgg
     2161 acggtgtgtg gcgcgccgcg ttcatcagca ggttctcgac gatccgggag atcatctccg
     2221 gatcgaccca ggcgctcacc gagcgcgtgt cgacgtgaac ctggcgcccg gccatcgcct
     2281 cggagtccca gacgagccgg gagatgagcc tgccgacggc gatctcctgc agcttgggct
     2341 cgaccacgcc gcgggcgaga cgctccgtgt cgagcaggtc c
//
LOCUS       DASUVE010000301         4971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_714002, whole genome shotgun sequence.
ACCESSION   DASUVE010000301 DASUVE010000000
VERSION     DASUVE010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714002"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VF036_06465"
     CDS             <1..425
                     /locus_tag="VF036_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6264907.1"
                     /translation="AALTDTPVPLIAPALVGVSPLLVLLFAWSYHSVSGDVYDLAREA
                     GLGPFRTWWTVAVPIRLPVVGVAAAIAFMVSWGNLTDPLLYVYDERWYTLPLGLASVA
                     QLPSTDHGLLLAAAVLATVPVLVTAFLIQRFVSDRRWA"
     gene            422..1702
                     /locus_tag="VF036_06470"
     CDS             422..1702
                     /locus_tag="VF036_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX6264908.1"
                     /translation="MTGSTRYAIAATVALAVVVAGCESEAEVPTRITVMVAGDPAEID
                     AYRAVVEGFDSSQDAVDAELQPFAERDELIARLGTSIAGGQPPDLFLMNYRFYGQFAA
                     PGALEPVGPFLDASSALSEGDFFETAMTPFRWDGVQTCLPQNVSSLVVYYNADLFRDA
                     GVSLPASGWSWDEMVDAAERLTRDDDDDGTVDVYGLGVNPEIIRVAPLIWSNGGTLVD
                     DETSPTRFALNGPAVAALDRFLDLRTRGVTPTDEEAEAEDFESRFLNGGLAMLMESRR
                     VVSTLRTITDFTWDVAGFPGMDEPASVLHSDAYCMTAASQEKDAAWTFLEFALGPEGQ
                     RIAAESGRTVPSLRSVAESDAFLDPEVDPAHSEVFVEQIPYLRSVPVISTWPEIEDTV
                     NGLLEEAYYRGGRALEVAIELTVSTRRQFARAEG"
     gene            1735..2586
                     /locus_tag="VF036_06475"
     CDS             1735..2586
                     /locus_tag="VF036_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6264909.1"
                     /translation="MASPFVIGVCALVIVPAAGSLVMAFSEWDLVRSPRFVGTANFRE
                     LIGDPVFRISLRNSLFFTLAAVPLRLVGALALALLLHHRSRLHAAGRVAVLVPTVIPD
                     AAYALVWIWILNPLHGPLNLALGAAGFPTPAWLTQPTPARWGVVLMSAFQLGEGFLLA
                     FVARRQMPDELYEIASLSGAGRWSRFIRVTLPVMAPVLLLIAMRDTIVTLQGTFVPAL
                     VVTEGGPPPYATTYLPLFIYRNAFEYLRYGYASAATVAMLVVTASILWLQFRVVIRWQ
                     GRFVPAP"
     gene            complement(2599..3630)
                     /locus_tag="VF036_06480"
     CDS             complement(2599..3630)
                     /locus_tag="VF036_06480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6264910.1"
                     /translation="MEARESIANAIRDHGPITFSEYMALALSGPGGFFERPPIGPDGH
                     FVTSPHVHPVFSELLGRAIVDLHDRLDRPTPLRLSEVGAGDGTLARRLREHLRGVKVV
                     YTAIDTSPGARAALGAIDGVEVAEELDGSPQLILANELMDNLPFRRLRGTASGTKEVK
                     VGLDGDRFVEHLDDPPDEVFTLADGEEAVLPEGATAFIEAVGARLAHPGYALLIDYGG
                     VGEPGGSPHGYRQHRPVAGLLERPGETDITCGLDFDLISEHAEKQGLIAFPSVTQRHA
                     LTALGFDHWIHEQLRRQAELLDRREGLDAVRTWSGRSRATLLVDPSALGRLRWLLLAT
                     PGLRGPSWI"
     gene            3668..4315
                     /locus_tag="VF036_06485"
     CDS             3668..4315
                     /locus_tag="VF036_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX6264911.1"
                     /translation="MSEEIRQAASLIGVRDGPGGPEVLVIERAPDQRFLPGYVAFPGG
                     AVDLADRALAERWFGDAEEGARAAAVRELAEEVGLAVTRDAVLGAGPDRSLASIDGSP
                     PATDALPEIARWIAPEQVPVRFDARYFAVRMDEGADPVPDGAEAAKAWWVSPVDLLAA
                     WDAGDVRLYWPTHYTMHALADRRDADDVLGLRLVTREPDDDELGRLPRSVFFQDR"
     gene            4312..>4971
                     /locus_tag="VF036_06490"
     CDS             4312..>4971
                     /locus_tag="VF036_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6264912.1"
                     /translation="MNGSLLPIRRVLAPNAGIYTLEGTNTWIVGSDPTFVIDPGPDMP
                     EHLADVAREAGSVGAVLVTHDHPDHAPGAIGFGRLVGAPVYAFRLAGATKLSAGQRVR
                     AGELDLTVIHTPGHTSDHVAFFEPGSGALFTGDTVLGRGTSFIDPPDGDLVQYLRSLR
                     RLMDLDARSIYPGHGPAVLHARDKLVEYLAHRDEREAQIVSVLAEGPRTIAEMVEVIY
                     AD"
BASE COUNT          685 a         1732 c         1713 g          841 t
ORIGIN      
        1 tcgcggccct caccgacaca cctgttccgt tgatcgctcc cgcgctcgtc ggtgtttcgc
       61 cgctcctcgt gctgctgttc gcgtggagct accactccgt gtccggcgac gtctacgacc
      121 tcgcccggga ggcgggactg ggaccgttcc gcacgtggtg gaccgtggcc gtgccgatcc
      181 ggctcccggt cgtgggggtc gcggcggcga tcgcgttcat ggtctcgtgg ggcaacctga
      241 ccgatccact gctctacgtc tacgacgaac gctggtacac gctgccgctc ggcctcgcgt
      301 cggtcgccca gctcccttcc accgatcacg ggctgctgtt ggccgccgcg gtcctcgcga
      361 ccgttcccgt gctcgtcacc gcgttcctca tccaacggtt cgtttccgac agaaggtggg
      421 catgaccgga tccaccaggt acgcgatcgc cgcgaccgtc gctctcgcgg tggtcgtcgc
      481 cggttgcgag agtgaagccg aagttccgac ccggatcacc gtcatggtcg cgggcgatcc
      541 tgccgagatc gacgcgtacc gagccgtcgt cgaaggcttc gacagctcac aggacgccgt
      601 cgacgcggag ctccagcctt tcgccgaacg agacgagctg atcgcccggc tcggcacgtc
      661 gatcgcgggc ggacagccgc ccgacctctt cctgatgaac taccgcttct acgggcagtt
      721 cgcggctccg ggtgcgctgg agcccgtcgg accgttcctc gatgcctcgt cggcgctctc
      781 cgaaggggac ttcttcgaga cggcgatgac gccgttccgt tgggacggcg tgcagacgtg
      841 cctgccccag aacgtgtcga gcctcgtcgt gtactacaac gcggacctct tccgtgacgc
      901 cggtgtatcg ctgcccgcga gcggatggtc ctgggacgag atggtcgacg ctgcggaacg
      961 attgacgcgc gacgatgacg acgacgggac ggtcgacgtc tacggcctcg gggtgaaccc
     1021 ggagatcatc cgcgtcgcgc cgctgatctg gtccaacggc ggaaccctcg tggacgatga
     1081 gacgtcaccg accagattcg cgctcaacgg gccggccgtc gcggcgctgg accgattcct
     1141 ggatctccgg acgcgcggcg tcacaccgac cgacgaggag gccgaggcgg aggacttcga
     1201 gagccgtttc ctgaacgggg ggctcgccat gctgatggag tcccggcgcg tcgtgtcgac
     1261 gctccggacg atcacggact tcacgtggga cgtcgccggc ttccccggca tggacgagcc
     1321 ggcgtcggtg ctccactcgg atgcctactg catgacggcg gcctcgcagg agaaggacgc
     1381 tgcgtggacg ttcctcgagt tcgccctggg acctgagggt cagcggatcg cggcggagtc
     1441 gggacgcacc gtcccgtccc tgcggtcggt ggcggagagc gacgcgttcc tcgatcccga
     1501 ggtcgatccc gcccactccg aggtgttcgt cgagcagatc ccgtacctcc ggtcggtccc
     1561 tgtgatctcc acctggcccg agatcgagga cacggtcaac gggctgctcg aggaggcgta
     1621 ctaccggggc ggccgggcgc tcgaggtggc gatcgagctc acggtgagca ctcgccggca
     1681 gttcgcgcgc gccgagggct gatggagcgg tccgagcgcc ggatcttgtg gctcatggcg
     1741 tcgcccttcg tgatcggggt ctgtgcgctc gtgatcgtcc cggctgcagg ctcactcgtg
     1801 atggcgttct cggagtggga cctcgttcgg tcgcctcggt tcgtcggcac cgccaacttc
     1861 cgcgagctga tcggtgatcc cgtgttccgg atctccttgc ggaacagcct gttcttcacg
     1921 ctggcggccg tgccgctcag gctcgtggga gcgctcgccc tcgccctcct gctgcatcac
     1981 cggagcaggc tccatgccgc gggccgcgtg gcggtcctgg tcccgacggt gatccccgat
     2041 gcggcctatg cgctcgtgtg gatctggatc ctcaatccgc tccacgggcc gctgaacctc
     2101 gccttggggg ccgcgggttt cccgacgccg gcgtggttga cccaacccac gccggcccga
     2161 tggggggtcg tgctgatgtc ggcgttccag ctcggcgagg gcttcctcct cgcgttcgtc
     2221 gcccggcgac agatgccgga cgagctgtac gagatcgcct cgctgagcgg tgcgggacgc
     2281 tggtcgaggt tcatcagggt gaccctcccc gtgatggctc cggtcctgct gctcatcgcg
     2341 atgcgcgaca cgatcgtcac gctccagggg acgttcgtcc ccgcgctcgt cgtgaccgag
     2401 ggagggccgc cgccgtacgc gacgacctat ctcccgctct tcatctaccg gaacgccttc
     2461 gagtacctgc gctacggata cgcctcggcc gccacggtcg cgatgctggt cgtcacggcg
     2521 tcgatcctct ggttgcagtt ccgggtggtg atccgatggc aggggcggtt cgtgccggcc
     2581 ccgtgagggc acgtccgctc agatccacga gggaccacgc agccccggcg tggcgaggag
     2641 gagccagcgg agccggccga gcgccgaagg gtccacgagc agcgtggccc ggctccttcc
     2701 gctccacgtt cggacggcgt cgagcccctc acgacgatcc aacaattcgg cctgtcggcg
     2761 gagctgctcg tggatccagt ggtcgaagcc gagcgcggtg agcgcgtggc gctgcgtgac
     2821 gctcgggaac gcgatcagcc cctgcttctc ggcgtgctcg ctgatgagat cgaaatcgag
     2881 gccgcacgtg atgtcggttt cgcccggccg ctcgaggagg cccgcgaccg gtcggtgttg
     2941 ccgatacccg tgcggcgaac cgcccggctc gcccacccca ccgtagtcga tcaggagcgc
     3001 gtagcccggg tgggcgagcc gggcccccac cgcctcgatg aaggccgtcg ccccctccgg
     3061 gagcaccgct tcctcgccgt cggccagcgt gaagacctcg tccggtggat cgtcgagatg
     3121 ctcgacgaac cgatcgccgt cgagcccgac cttgacctcc ttcgtccccg aggctgttcc
     3181 gcgcagtcgc cggaagggca ggttgtccat cagctcgttc gccaggatca actgcggcga
     3241 gccgtcgagc tcctccgcga cctcgacgcc gtcgatcgcc ccgagcgctg cacgtgcgcc
     3301 ggggctcgtg tcgatcgccg tgtagaccac cttcacgccg cgcaggtgct ccctgagccg
     3361 ccgcgcgagc gtcccgtcgc cggcccccac ctccgacagc cggagcggcg tcggtcggtc
     3421 cagccggtcg tgcagatcga cgatcgcccg gccgaggagc tccgagaaga ccggatggac
     3481 gtgcgggctc gtgacgaaat ggccatccgg tccgatcggt gggcgttcga agaagcctcc
     3541 cgggcccgag agcgcgagcg ccatgtactc cgagaaggtg atgggtccgt gatccctgat
     3601 cgcgttggcg atcgactcgc gcgcctccac gggcccaagc gtacggccct gggtagcatc
     3661 gggatccgtg agcgaggaga tccgtcaggc ggcttcgctg atcggtgtcc gagacgggcc
     3721 aggcgggccc gaggtgctcg tgatcgagcg tgcgcccgat cagcgtttcc tccccggcta
     3781 cgtggccttc cccggcggcg cggtcgacct cgcggacagg gcgttggcgg agcgttggtt
     3841 cggtgacgcg gaggaaggag cccgggccgc cgcggtgcgc gagctggccg aggaggtcgg
     3901 cctcgcggtg acgcgggatg ccgtcctcgg cgcgggtccc gatcgctccc tcgcatcgat
     3961 cgacggatcc ccgccggcga ccgacgcgct cccggagatc gctcgatgga tcgcgccgga
     4021 acaggttccc gtgcgcttcg acgcccggta cttcgccgtc cgcatggacg agggtgccga
     4081 tcccgtaccg gacggcgccg aagcggcgaa ggcctggtgg gtctcgccgg tggatctctt
     4141 ggccgcctgg gatgccggtg acgtacggct ctactggccg acccactaca cgatgcacgc
     4201 cctcgcggat cgacgcgacg cggacgacgt cctcggtctg cggctcgtca cccgcgaacc
     4261 cgatgacgac gagctgggcc ggttgccgcg atcggtcttc ttccaggacc gatgaacggc
     4321 tccctgctgc cgatccgaag ggtgctcgct ccgaacgccg gcatctacac gctggaaggg
     4381 acgaacacct ggatcgtcgg gagcgatccg acgttcgtga tcgatccggg acccgacatg
     4441 cccgagcatc tggcggacgt cgcgagagag gccggatcgg tcggtgcggt actcgtcacg
     4501 cacgaccatc cggaccacgc gccgggcgcg atcgggttcg gccggctcgt cggagcgccc
     4561 gtgtacgcgt tccggctcgc gggcgcgacc aagctctccg ccggtcagcg cgtgcgcgcc
     4621 ggcgagctcg acctcaccgt gatccacacg ccgggtcaca cgtccgacca cgttgccttc
     4681 ttcgagcccg gcagtggcgc gctgttcacc ggcgatacgg tgctcgggag aggtaccagc
     4741 ttcatcgatc cgcccgacgg ggacctcgtc cagtacctgc ggtcactacg acgactcatg
     4801 gatctggatg cccgatcgat ctacccgggt cacggccccg ccgtgctcca cgctcgcgac
     4861 aagctggtcg agtacctcgc gcaccgagac gagcgggaag cgcagatcgt gtcggtgctc
     4921 gcagaaggcc cgcgaacgat cgccgagatg gtcgaggtga tctacgcgga t
//
LOCUS       DASUVE010000302         3662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_716030, whole genome shotgun sequence.
ACCESSION   DASUVE010000302 DASUVE010000000
VERSION     DASUVE010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716030"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VF036_06495"
     CDS             complement(<1..217)
                     /locus_tag="VF036_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEX6264913.1"
                     /translation="MNPLPQVPLSVVADAVRRAEIRGDAGVQIREVAFDSREVPTGAL
                     FFCVPGANIDGHGHARDAIRAGAVALVV"
     gene            complement(288..1994)
                     /locus_tag="VF036_06500"
     CDS             complement(288..1994)
                     /locus_tag="VF036_06500"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX6264914.1"
                     /translation="MRRPPAGRLVALLGMMVLALAGVGTRLVILQVGDHRAFAAQGLD
                     QRVHLIDLPAERGAIVDRTGVPLAITLEARDVYADPRYVTDPIGEAARIAKILDLQPR
                     QVERLLRTPDSTFVYLGRQVDAEDAEKLESLGLPGIGFLDVPKRYYPAGALAPQVLGF
                     VGVDATGLAGLEFEYQDVLAGSPGTRTVELSADGLPIASGIEVVEPAIRGETLVTTLD
                     REVQYLAQEALQRAVKENGAKGGTIVVMDARTGDVYAMASYPWFDPNDFETAERDAIR
                     NRAVTDMFEPGSVNKVVTAAAALETGAVGLRKVFRVPSWMRVGGYTVNDSHAHPIQDM
                     TLGDIVAQSSNIGSAMIAERVGSADLASVLARFGYGTPTGIGFPGEAGGVVPDLRNWT
                     DITRTTVSYGQGISMTPLQMAAVYATVANDGMWVQPRLVRGTSGSDGRVQQTRPDEPR
                     RVLSEETATMLTRMLASVVSSGTGGAADIPGYQVAGKTGTARKLVEGRYVRRYMASFV
                     GFFPASDPRVVIAVSIDEPRTIYGGVAAAPVFSEIARHVIQRLAIPAAPPVSLPPFAA
                     DP"
     gene            complement(1991..2443)
                     /locus_tag="VF036_06505"
     CDS             complement(1991..2443)
                     /locus_tag="VF036_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264915.1"
                     /translation="MSLPAREAERWAPAPQHQPRRRAVSPARPPATRPPIPTPPHPHR
                     RARRGHHTAFWVLTAVVVSSLVVGLVSVNAMRVDAAYRSRSLAEEIRTMSDERRTLVN
                     DVARLSSPSRVGRWAVRRGLVHPAAGDVVILQVPGFSHPTEPTAEAGE"
     gene            complement(2440..3327)
                     /gene="rsmH"
                     /locus_tag="VF036_06510"
     CDS             complement(2440..3327)
                     /gene="rsmH"
                     /locus_tag="VF036_06510"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX6264916.1"
                     /translation="MANDERSHEPVMVAEVVECLLPEGSGTGRRVADMTVGSGGHAAA
                     LLDAGVEHVIGFDRDPEALRIARERLAAYGDRFTVVHRRFSQVGEAEATGPLHGVLFD
                     LGVSSMQLDEPARGFGYRVDGPLDMRMGDGDITAADLVNDLPEVELADLLYEFGQEHR
                     SRRVAAAIGRARQRARIETTDQLAGVVASALGRRPGGPHPARRTFQALRIAVNRELEE
                     LATSLPRAVGLLGPGGRVVVLSYHSLEDGIVKRAFRDDERLRVLTKKPLQPSDEERAR
                     NPRARSARFRAAERIEEAA"
     gene            complement(3445..>3662)
                     /locus_tag="VF036_06515"
     CDS             complement(3445..>3662)
                     /locus_tag="VF036_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX6264917.1"
                     /translation="PQRLRDAVGINKEVVVLGVRERIEIWDRAEFERYRDAHADAYEN
                     GDLEPRGPRPVVGSHSGFDRAPRTTEG"
BASE COUNT          555 a         1324 c         1279 g          504 t
ORIGIN      
        1 ccacgacgag cgcgacggct cccgcgcgga tcgcgtcgcg agcgtgaccg tgtccgtcga
       61 tgttcgcgcc cgggacgcag aagaagagcg cgccggtggg gacctcgcgg gaatcgaacg
      121 cgacctcgcg gatctggaca cctgcgtcac cgcggatctc ggcacgacgc acggcgtccg
      181 cgaccacgga cagcggcacc tggggaagag ggttcaccgc ggtccagcgt accggcggcg
      241 cacgcccgaa cccgccaagc ggacccggcg aaccgcggga cgaccgatca gggatccgcc
      301 gcgaacggcg gcaggctcac gggcggtgcg gccgggatcg cgaggcgctg gatcacgtgt
      361 cgcgcgatct cggagaagac cggggcggcc gccacacccc cgtagatcgt ccgaggctcg
      421 tcgatcgaca ccgcgatcac cacccgggga tccgacgcgg ggaagaaccc cacgaacgac
      481 gccatgtacc gccggacgta cctgccctcg accaacttcc gggccgtgcc ggtcttgccg
      541 gcgacctgat agccggggat gtccgccgcg ccgcccgtgc ccgaagacac cacggaggcg
      601 agcatgcggg tgagcatcgt cgccgtctcc tccgagagga cccgacgtgg ttcgtcggga
      661 cgtgtctgct ggacccgccc atcggagccg gaggtgccgc ggacgagccg gggctgcacc
      721 cacatcccgt cgttggccac cgtggcgtag acggcggcca tctgcagcgg cgtcatcgag
      781 atgccctgtc cgtaggacac ggtcgtccgc gtgatgtcgg tccagttgcg gagatcgggg
      841 acgacgcccc cggcttcccc cgggaagccg atccccgtgg gggtcccgta gccgaaccgg
      901 gcgagcacgg aagcgagatc cgcgctcccc acccgctcgg cgatcatcgc ggacccgatg
      961 ttgctggact gggcgacgat gtcgccgagg gtcatgtcct ggatgggatg ggcgtgggag
     1021 tcgttgacgg tgtagccgcc gaccctcatc cacgacggga cacggaagac cttgcggaga
     1081 cccaccgcgc cggtctcgag ggccgccgcc gccgtcacga ccttgttgac cgatcctggc
     1141 tcgaacatgt cggtgacggc gcggttccgg atcgcgtccc gctcggcggt ctcgaagtcg
     1201 ttggggtcga accagggata ggaggccatc gcgtagacat cgccggtccg cgcatccatg
     1261 acgacgatcg tcccgccctt cgcgccgttc tccttcaccg cgcgctgcag cgcctcctga
     1321 gccaggtatt ggacctcgcg gtccagggtc gtcacgagcg tctctccgcg gatcgccggc
     1381 tccacgactt cgatcccgct cgcgatcggc aacccgtctg ccgacaactc caccgtgcgg
     1441 gttccgggtg aaccggcgag gacgtcctgg tactcgaact cgaggcccgc gagccccgtg
     1501 gcgtcgaccc cgacgaagcc gagcacctgc ggcgcgagcg cgcccgccgg gtagtagcgc
     1561 ttggggacgt cgaggaaccc gatgcccggg aggccgagcg actcgagctt ctcggcgtcc
     1621 tcggcgtcga cctggcgccc gaggtacacg aacgtggagt cgggtgtgcg cagcagccgc
     1681 tccacctgac ggggctggag gtcgaggatc ttcgcgatcc gcgcggcttc gccgatcggg
     1741 tcggtcacgt accgagggtc ggcgtagacg tcccgagcct ccagggtgat cgcgagcgga
     1801 acccccgtcc gatccacgat cgcgccgcgc tccgccggca ggtcgatcag atgtacccgt
     1861 tggtccaggc cctgcgcggc gaacgctcgg tgatcaccga cctgcaggat caccaaccgg
     1921 gtccccacgc ccgccagtgc cagcaccatc atgccgagca gcgcgacgag tcgaccggcg
     1981 ggcggacgcc tcactccccc gcctcggccg tcggctcggt gggatgcgag aagccgggca
     2041 cctgaaggat caccacgtca cccgcggcag ggtgcacgag cccccggcga accgcccagc
     2101 gaccgacccg ggacggcgac gacaggcggg cgacgtcgtt cacgagggtc cgccgctcgt
     2161 cgctcatcgt tcggatctcc tcggcgaggg atcggctccg ataggcagcg tccacccgca
     2221 tcgcgttcac ggagacgagg ccgaccacga gcgaggacac gaccaccgcc gtgaggaccc
     2281 agaacgcggt gtggtgcccg cgacgcgccc gccgatgggg atggggaggg gtggggatcg
     2341 gcgggcgcgt cgcgggcggc cgtgcggggc tgaccgcacg acgtcgaggt tggtgctgcg
     2401 gagcgggcgc ccaccgctcc gcctcgcggg ccgggaggct catgccgcct cctcgatgcg
     2461 ctcggcggcg cggaacctgg cgctccgcgc acgagggttg cgggcgcgct cctcatcaga
     2521 gggctggagc ggcttcttgg tgaggacacg gaggcgctcg tcgtcgcgga acgcgcgctt
     2581 gacgatcccg tcctccaggg agtgatagga aaggacgacc acgcggccac cggggccgag
     2641 gagcccgacc gcccgaggca gggaggtggc gagctcctcg agctcccggt tgaccgcgat
     2701 gcggagcgcc tggaacgtcc gacgcgcggg gtgggggccc cccggacgtc gtcccagagc
     2761 actcgcgacc acaccggcca gttggtcggt ggtctcgatc cgagcccgct gtcgcgcacg
     2821 accgatcgcc gcggcgacgc gacgcgagcg gtgctcctgc ccgaactcgt agagcagatc
     2881 ggcgagctcg acctccggaa ggtcgttcac gaggtccgca gcggtgatgt caccgtcgcc
     2941 catgcgcatg tcgagcggtc cgtccacgcg gtacccgaac ccgcgggcgg gctcgtcgag
     3001 ttgcatcgac gacactccca ggtcgaagag gaccccgtgc agcggccccg tggcttcggc
     3061 ctctccgacc tgggagaagc gtcgatgcac caccgtgaag cggtccccgt acgcggccag
     3121 gcgctcgcgc gcgatccgca gtgcctcggg atcacggtcg aacccgatca cgtgctccac
     3181 gccggcgtcg agcagcgcgg ccgcatggcc cccggagccc acggtcatgt cggcgacccg
     3241 tcgcccggtc cccgacccct cgggaagcaa gcactcgacg acctcggcca ccatcacggg
     3301 ctcgtgcgac cgctcgtcgt tcgccacgtc acccgatctg tcccgccagg accccgaggc
     3361 cgatcgacgc gagcaacgtc agcgccgtca tcccgagcaa cgagaggctc tgtcgaacct
     3421 ccgtcgaaag cttcgtctgc atcctcatcc ctccgtcgtc cgtggtgctc tgtcgaaacc
     3481 ggagtggctt cccacgaccg gccgtgggcc tcggggctcg aggtcgccgt tctcgtaggc
     3541 gtccgcatgt gcgtcccggt accgctcgaa ctcggctcgg tcccagatct cgatccgttc
     3601 gcggacgccg agcacgacca cctctttgtt gatgccgacc gcgtcccgca gtcgctgtgg
     3661 ga
//
LOCUS       DASUVE010000303         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_338083, whole genome shotgun sequence.
ACCESSION   DASUVE010000303 DASUVE010000000
VERSION     DASUVE010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338083"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VF036_06520"
     CDS             <1..1134
                     /locus_tag="VF036_06520"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX6264918.1"
                     /translation="VAVTVVGDTLDEANESFFLELSNPVGATITDDRGEATITDDDAS
                     PTVAIVPSISVPEGNTGDHPSASLDVTLSAVSGRDVAVSYATVDVTATTADGDYVAKS
                     GTIDFAPGETQQTIVVTVVGDDDVELDETFNVQISSPVHATLGNATAVVTITGNDPIP
                     PGSAVLNISGASVREGALGTTRVLTFTIVRSGETTTALDVDFATTNGTATDPSDYLAA
                     SGNVAFAANETSKTVDVTIVGDRRLERKETFFVSLLDPSVGAAIESGQAAGHIVNDDT
                     RTSLVVTKGANRILARGRLSPARPGNRMVVRLFRKRNGVWVRLQTRRPRLSGRTDLNG
                     DGFTDSTYRTRFGRPRPGTCKVIAQFVGGPTFGPSKAIRIFRC"
     gene            1578..2162
                     /locus_tag="VF036_06525"
     CDS             1578..2162
                     /locus_tag="VF036_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX6264919.1"
                     /translation="MRVGRILGLVVTTGFLISTLAAGTSSGVPPGCTIIGTPGRDVLS
                     GTDGPDVICGLGGGDRIHGKGGNDVLIGGDGGDLLFGGAGKDKLRGGDGLDLGLGGGG
                     RDVARGGRGQDSIIGGPKADRIFGGPGRDGQLWGQSGTGDFVSGGAGDDSCVSTWDRD
                     GDDVVVGGPGHDIFFADPGDTRRSVEQSAGCIGD"
     gene            complement(2215..>2566)
                     /locus_tag="VF036_06530"
     CDS             complement(2215..>2566)
                     /locus_tag="VF036_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264920.1"
                     /translation="DRDGDRALREISLRRWCPLVNMVVLAGTVPLLVLARERGHRPTR
                     RRRARRTRGAGAARRLERSDADRAALIGRLHVRDDAAWLAELMDLEDEVGEVARLRLV
                     DALRQSLRSAGAAS"
BASE COUNT          438 a          840 c          889 g          399 t
ORIGIN      
        1 gttgccgtga cggtggtcgg cgataccctc gacgaggcga acgagtcgtt cttcctcgag
       61 ctctcgaacc cggtcggggc cacgatcacg gacgaccgcg gagaagcgac gatcacggat
      121 gacgacgcat cgcccactgt cgccatcgtg ccgagcatct cggtgccgga gggcaacacc
      181 ggcgatcacc cgtccgcgtc cctcgatgtc acgctgtcgg cggtgagtgg gcgggacgtg
      241 gcggtgtcgt acgccacggt cgacgtcacc gcgacgacgg cggacgggga ctacgtggcg
      301 aagtcgggca cgatcgactt cgctccggga gagacgcagc agacgatcgt ggtcacggtc
      361 gtcggggacg acgatgtgga actggatgag acgttcaacg tccagatctc atcgccggtc
      421 cacgcgaccc tcgggaacgc gaccgcggtg gtcacgatca cgggcaacga tccgatcccg
      481 ccgggctcgg cggtcctgaa catctcgggg gcatccgtgc gtgagggcgc gctcggcacc
      541 acgcgcgtcc tgaccttcac gatcgtgcga tcgggagaga ccacgacggc cctggacgtg
      601 gacttcgcga cgacgaacgg aacggccacc gacccgtccg actacctggc ggcgagcggt
      661 aacgtcgcgt tcgcagcgaa cgagacatcc aagacggtcg acgtgaccat cgtcggagac
      721 cgtcgcctcg aacgcaagga gacgttcttc gtcagcctgc tcgatccttc cgtcggggcg
      781 gcgatcgaga gcggacaggc ggcggggcac atcgtcaacg acgacacacg aacgagtctc
      841 gtcgtcacga agggagccaa tcgcatcctc gcgcgtggcc gactcagtcc cgccagaccc
      901 ggcaacagga tggtggtgcg gctcttccgc aagaggaacg gtgtgtgggt tcgactccag
      961 actcgccgtc cgcggttgag cgggcggacc gacctgaacg gggacgggtt cacggacagc
     1021 acctaccgca cccggttcgg gagacctcgg ccaggcacct gcaaggtgat cgcccagttc
     1081 gtcggaggac cgaccttcgg gcccagcaag gccatcagaa tcttccgctg ttgacaggcg
     1141 gtctcaggga gcgaagcggg ttgccgagcg gtcggacgcc ccgttcgacc ggctggtggc
     1201 tcgcctccac tggaggaacg ggcggatgtg accatccgcg atgggtcgcc tatcctcctc
     1261 ggttgagggc gcgtcgctca gtgggagagc gcttgcttca cacgcaccgg gggctaaacg
     1321 gtgtgccgac ctgcgtaaac gcacgtcgac gctgagcggg actgccaaac ttatccgctg
     1381 ctcaagttgc tcggggggac gcgcttctgg cacagtcgcg gaatgagtga tcagccgcag
     1441 cttgagctcc gggtacctcc tgattgggag cgcggagcct acgtcaacat ggcgagtgtt
     1501 cggcacacgc cgtatgagtt caccatcacg acccggtcat cggacgggaa ctgcttcggc
     1561 gtcgggggag gagaatgatg cgggtaggtc ggattctcgg tctcgtcgtc accaccggct
     1621 tcctcatctc gaccctcgcc gccggcacgt cttcaggcgt gccgcccggc tgcaccatca
     1681 tcggaacgcc gggccgcgac gtcctctccg gcacggacgg ccccgacgtg atctgcggtc
     1741 tcggtggtgg ggacaggatc cacggcaagg gagggaacga tgtcctcatc ggcggcgacg
     1801 gcggcgactt gttgttcggc ggcgcaggca aggacaagct gcgaggaggg gatggcttgg
     1861 acttgggcct cggcggcggg ggccgcgacg tggcgcgcgg cggcaggggt caggactcca
     1921 tcatcggcgg accgaaggcc gacaggatct tcggtggtcc gggccgtgac ggccagctat
     1981 ggggccagtc cgggaccggc gacttcgtca gcggtggggc cggcgacgac tcctgcgtat
     2041 cgacgtggga tcgcgacggc gacgacgtcg tggtcggtgg cccgggtcac gacatcttct
     2101 tcgccgatcc aggagacacg cgccggagcg ttgagcagtc ggcgggctgc atcggcgact
     2161 aagatcggtt cctcaagaca gtccgcctcg atggacgcct cgccctcaac aaactcatga
     2221 cgcagcaccc gcacttcgca ggctctgtct gagcgcgtcg actagccgga gccgggctac
     2281 ctcacccacc tcgtcctcga ggtccatcag ctcggccagc cacgcggcgt catcccggac
     2341 gtgcagccgg ccgatcagcg cggcccggtc ggcgtccgac cgctcgagcc ttcgagcagc
     2401 tcccgcgccg cgggttcgcc gagcgcggcg acgacgcgtc ggccgatgtc ctcgctcacg
     2461 cgccaagact agaaggggga ccgtgcccgc cagcacgacc atgttgacta agggacacca
     2521 cctgcggagg ctaatctccc tcagggcgcg atctccgtct cgatca
//
LOCUS       DASUVE010000304         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1280978, whole genome shotgun sequence.
ACCESSION   DASUVE010000304 DASUVE010000000
VERSION     DASUVE010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280978"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VF036_06535"
     CDS             complement(<1..687)
                     /locus_tag="VF036_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX6264921.1"
                     /translation="MTSELAGARKRLETADGALDIYSLPWLAEAGVADVSRLPLTVRI
                     LVENLLRRVGSRDVADEDVRALARWPAPAADVAFMPGRVLMQDFTGVPAVVDLAAMRG
                     AVARAGGDPAGVDPLVPVDLIIDHSVQVDLFRSRDAYEANIDWEYRRNGERYALLRWA
                     QQAFDGLRVVPPGAGICHQVNLEHLGQVVSVRDGVAFPDTLMGTDSHTTMVNGLGVLG
                     WGVGGIEAEAA"
     gene            complement(786..1304)
                     /locus_tag="VF036_06540"
     CDS             complement(786..1304)
                     /locus_tag="VF036_06540"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX6264922.1"
                     /translation="MSSSGARTVSDSEVTLTQMMEVTDANVAGNVHGGAIMRLVDTAA
                     ALAAIKHSGGLALTVGIDEMSFLEPVHIGEVVVLKACVNDVGRSSMECGVRVEAQDPL
                     TGAVRHVNSAYLVFVAVDAAGRPRTVRPLLAETDTQRRRQQEAKLRRERRIAHKEAID
                     IARAAKDAPEGV"
     gene            1532..2101
                     /locus_tag="VF036_06545"
     CDS             1532..2101
                     /locus_tag="VF036_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264923.1"
                     /translation="MPTEFIADLPPPDLPPEELIPTGADVTGEWFAFTHRGAAVLIAW
                     VEPGTDYARLPRGFAVWRHFDAGPHWRTALVTRHDPEDGIQEIQITTGDLTGDGSDDA
                     LIFEGVGGSGACGRWSVVDLARVQETYRMDLCDGRIEPGPPGAPGLVLTGAVYRQGDA
                     HCCPSAIRRTTLTWTGTMWRVTDRTLVED"
     gene            2265..>2510
                     /locus_tag="VF036_06550"
     CDS             2265..>2510
                     /locus_tag="VF036_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264924.1"
                     /translation="MLTGNGLARRVHIALIVLLVLGVAGSFWMSVRAQATARDAGAEQ
                     AEALVDSSLTLVFDPEDLQRDLPSERLRELTRSIDDVV"
BASE COUNT          365 a          885 c          886 g          374 t
ORIGIN      
        1 cgccgcctcg gcttcgatgc cgccgacgcc ccatcccagg accccgagcc cgttgaccat
       61 cgtggtgtgg gaatccgtgc ccatcaacgt gtcggggaag gccacgccgt cccggacgct
      121 cacgacctga ccgagatgct ccaggttcac ttgatggcag atgccggcgc cgggagggac
      181 gacacggagt ccgtcgaagg cctgctgggc ccagcggagc agcgcgtagc gctcaccgtt
      241 gcgccggtac tcccaatcga tgttggcctc gtacgcgtcc cgggagcgga agaggtccac
      301 ctggaccgag tggtcgatga tcaggtcgac cggcacgagc ggatcgacgc cggcgggatc
      361 cccgcccgca cgggcgaccg cgcctcgcat cgcggccagg tcgacgaccg cggggacccc
      421 cgtgaagtcc tgcatcagga cccggccggg catgaacgcc acgtccgccg ccggtgcggg
      481 ccaccgcgca agcgcgcgca cgtcctcgtc cgcgacgtcc ctcgaaccga cccgacggag
      541 gaggttctcg acgaggatgc ggaccgtcag cgggaggcgg ctgacgtcgg cgacgcccgc
      601 ctccgcgagc cagggaagtg agtagatgtc gagggctcca tcggcggtct ccagccgctt
      661 ccgggcgcct gcgagttccg aagtcatccg atgggtcctc tcgtggtcgt ctcgttcctc
      721 ttccggccat cctcccacgc agacggagga accccgatgg gagggccgtc gccccgggtg
      781 agacgtcaga cgccctcggg agcgtccttc gcggcccgag cgatgtcgat cgcctccttg
      841 tgggcgatcc ggcgttcgcg ccggagcttc gcctcctgct ggcgacgccg ttgcgtgtcc
      901 gtctcggcga gcagcggccg gaccgttcgt gggcggccgg ctgcgtcgac ggcgacgaag
      961 acgaggtacg cgctgttcac gtgccggacc gccccggtga gcgggtcctg ggcctcgacc
     1021 cggaccccgc attccatcga gctccggccg acgtcgttca cgcatgcttt gaggacgacc
     1081 acctcaccga tgtggacggg ttcgaggaac gacatctcgt cgatcccgac ggtgagggcc
     1141 agtcccccgg aatgcttgat cgcggccagg gcggcggccg tgtccacgag gcgcatgatc
     1201 gcgccgccgt ggacgttccc ggcgacgttc gcgtcggtga cctccatcat ctgggtgagg
     1261 gtcacctccg aatcggagac ggtgcgcgcg ccggacgagg acacgtgcgc atcctacgcg
     1321 ggcgctcgga gggggtcgcc ggaacgccga tagcatccgg gcgatgtgga tccgggccgg
     1381 agcgttgacc gttgcgctcg cggccgcggg gtgcgcgggg gaaggtccgg cgccatcgac
     1441 tccgacaccg cgcacgagcg tgacctctcg cggtcccgaa ccgtctccca gtcccgaacc
     1501 gagtccgagc tccgacccgg aggccggggc catgccgacc gagttcatcg ccgacctccc
     1561 acctcccgac ctcccccccg aggagctgat cccgaccggt gcggacgtga ccggcgagtg
     1621 gttcgcgttc acccatcggg gcgccgcggt gctgatcgcg tgggtggagc ccggcaccga
     1681 ctatgcccgg ctcccccgag gcttcgccgt gtggcgccac ttcgatgcgg gaccacattg
     1741 gcggacggcg ctcgtcacgc ggcacgatcc cgaggacggc atccaggaga tccagatcac
     1801 cacgggagat ctgaccggcg acggttcgga cgatgcgctg atcttcgagg gggtcggtgg
     1861 ctccggggcg tgcggacgat ggtccgtggt ggatctcgcc cgcgtgcagg agacgtaccg
     1921 gatggatctg tgcgacgggc gcatcgagcc ggggccgccg ggcgcgcccg gcctcgtgct
     1981 caccggagcg gtctaccggc agggcgacgc ccattgttgc ccgagtgcca tccggcggac
     2041 gacgctgact tggacgggca cgatgtggcg cgtcacggac aggacgctcg tcgaggattg
     2101 acgtggggcg tcggcccgtc cgacgatccc cgaagccgcc gccatccgcc ctaggtcctt
     2161 cggccacccg aatgaccaag aggaatcccc cggtcggcac tgaacccgct ccggagccgt
     2221 gccgataacg aggccgggac accgcacagg gggctggtac gtagatgctg actgggaacg
     2281 ggctggcacg cagggttcac atcgcactca tcgtgctgtt ggtgctcggc gtcgccggct
     2341 cgttctggat gagcgtccgc gcgcaggcga cggctcgcga cgccggcgcc gagcaggcgg
     2401 aggctctcgt cgacagttcg ctcaccctcg tgttcgatcc cgaggacctg caacgagacc
     2461 ttccttccga gcggctccgc gagctcaccc gctcgatcga cgacgtcgtc
//
LOCUS       DASUVE010000305         5382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1469710, whole genome shotgun sequence.
ACCESSION   DASUVE010000305 DASUVE010000000
VERSION     DASUVE010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469710"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1290
                     /locus_tag="VF036_06555"
     CDS             61..1290
                     /locus_tag="VF036_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6264925.1"
                     /translation="MPSAEPLVVIAGRLLDVTTGHVLTDRALVLDGDRIRDVVEGGAA
                     PQGRTIDLSGWTVLPGMIDCHAHLIGELDDGQGYAYLVTRSSAQEALTGVTNARATLL
                     GGFTTVRDVGTFHAFVDLALREAIEEGRLVGPRMMCAGAYVTCPGGGGDITGLPPDVP
                     VPSELRIGVSTGPDQVRANVKRIFDAGADFIKVIATGAVLTSGTNPGEPELTEEEIRA
                     AVEEAEARGTHVAAHAHGAEGAKRAIRAGVRSIEHGCLIDDETIDLMLERGTYLVCDM
                     FDGDWMIQEGPRLGYSDEVMRKTEMTNDAQRENFAKAVKAGVKIAFGTDSGIYPHGMN
                     ARNLAFHVRFGQTPMEAIRSATLGAAELLGWDDRVGSLRPGLFADLIAVDGDPLDDVT
                     VLEDVPFVMKGGQVVKG"
     gene            1351..2679
                     /locus_tag="VF036_06560"
     CDS             1351..2679
                     /locus_tag="VF036_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6264926.1"
                     /translation="MREVQPLSAAEIVEKSKRYTLYDWQAQSKASPIAVDRAEGVYFW
                     DVDGTRYLDFNSQLMGVNIGHGDKRVADAIARQAEKLPYISPFMAYETRARLGEKLAT
                     LWPGDLEKSFFTLGGAEANENAIRIAKAFTGRQKILARYRSYHGATYATINLTGDPRR
                     WANENPPMPGIVHVLDPYRGTARGTEDAATALATLEETIELEGPRTIAAFILETVTGT
                     NGILIPPDGYLEGVRDLCNRHEILLIADEVMCGFGRTGEWFAVNHYGVVPDLMTAAKG
                     LTSSYLPLGAVAIKPEIAAHFEENVFYGGLTYNSHPLSCAAAIAAIQVLEEDDLVGNA
                     KRLDPVMRRHHEELMAKHPSVGRVRNIGLFGIIELVKNRDTMEPLSPYNVVNETMQAV
                     NKGLLQRGLFTMIRFNGIMTNPPLCITEEQLDDGFAIIDEVLDIADAATG"
     gene            complement(2687..3010)
                     /locus_tag="VF036_06565"
     CDS             complement(2687..3010)
                     /locus_tag="VF036_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6264927.1"
                     /translation="MDTDRAVCESFQHAAQLVGQRWVPQIVWVLLGGPRRYSVVRTAI
                     PEISDTVLSDRLRDLESAGILTRAVTPSTPVLIEYALTDRGRELAKVIDGLAKWADRW
                     AAVGA"
     gene            3148..3714
                     /locus_tag="VF036_06570"
     CDS             3148..3714
                     /locus_tag="VF036_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX6264928.1"
                     /translation="MADSTKEIGGLPLPPPGKYELDIAHTAVEFVARHIFTKVRGRFT
                     DFSGWIDVAENPEESSAHVEIKTASIQTNTEQRDEHLKSDDFLNVEKWPVMTFASTGV
                     RHTGGATFELDGELTIRDVTRAVTLVGEYLGTETNPYGKTVLAATAKTTLEREDWNVS
                     WNMVLETGGFLVSKAIDLEIEVEALKTG"
     gene            complement(3720..4307)
                     /locus_tag="VF036_06575"
     CDS             complement(3720..4307)
                     /locus_tag="VF036_06575"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HEX6264929.1"
                     /translation="MRGQSGDVVGTEDFRCAPGPMGWRYVSEIERDEPTPHRETVDVV
                     VDTSWRIVRTRIETGSHATLLEARRDSLEGIRDGHKVEMAWGPEMHLDYLSPAFNAIT
                     AQRLEGSSEIDVLFLEPVTLEPVPMRQRYELIGRESVETPSGTFLAARWGYTALATGW
                     TSDLWVADDVVVRYDRAFELISYEAGASGPLPTPP"
     gene            complement(4330..>5382)
                     /locus_tag="VF036_06580"
     CDS             complement(4330..>5382)
                     /locus_tag="VF036_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6264930.1"
                     /translation="ELPLDIVKKLGELGICGVPIPEEHGGLGADAVTWALVIEELGRA
                     DASVAVTVSVASGLAAGMIARYGSTEQRQRWLPPIAAGEMLAGFGLTEPGSGSDAAAL
                     RTRARLDGRSWVIDGSKSFITNSGSQITGFVVVACRTEDDAGANGVSTVVVPADAPGF
                     SVGPPYRKLGWRSSDTHELSFEGCRVPEDHLLGERGKGLAQCLAALDDGRISIAALSV
                     GLAQGCLDASLSYAKDREAFGRPIGNYEAIAFKLADMRAAVEASRLLTYRAAWLKDQG
                     RPYTTEAAIAKLRASETAVDVAREAIQVHGGYGYTEEFPVARAYRDAKVLEIGEGTSE
                     ILRLVIARDLGLQPQV"
BASE COUNT          904 a         1831 c         1837 g          810 t
ORIGIN      
        1 cacccggcga aggtcgcgag gcctcccccg gctgggggag gccgagagta tgctccgccg
       61 atgccatcgg ccgaaccgct cgtggtgatc gccggccgtt tgctcgacgt gacgaccggc
      121 catgtgctca ccgaccgcgc cctcgtgctc gacggcgacc gcatccgcga cgtggtcgag
      181 gggggtgccg ccccccaggg tcggaccatc gatctgtccg ggtggacggt cctgcccggc
      241 atgatcgatt gtcacgcaca tctgatcgga gagctcgacg acggtcaggg gtacgcttac
      301 ctcgtgacgc gctcctccgc gcaggaggcc ctcaccgggg tgacgaacgc gcgcgccacc
      361 ctgctcggtg gcttcaccac cgtccgcgac gtggggacgt tccacgcgtt cgtggacctc
      421 gcgctccgtg aggcgatcga ggaaggaagg ctcgttgggc cccggatgat gtgtgccgga
      481 gcgtatgtga cgtgccccgg cgggggcggg gacatcaccg gcctcccgcc cgatgtgccg
      541 gtgccgagcg agctccggat cggcgtgtcc accggccccg atcaggtgcg cgccaacgtc
      601 aagcggatct tcgacgcggg cgccgacttc atcaaggtga tcgcgacggg cgcggtcctc
      661 acgtccggga cgaacccggg cgagcccgag ctgaccgagg aggagatccg cgcggcggtt
      721 gaggaggccg aggcccgggg gacgcacgtc gcggcccacg cgcacggtgc cgagggcgcg
      781 aagcgggcga tccgcgccgg cgttcgctcg atcgagcacg gctgtctcat cgacgacgag
      841 acgatcgatc tgatgctcga gcgagggacg tacctcgtct gcgacatgtt cgacggcgac
      901 tggatgatcc aggagggacc ccgcctcggc tactcggacg aggtgatgcg gaagaccgag
      961 atgacgaacg acgcgcagcg cgagaacttc gccaaggcgg tgaaggcggg cgtcaagatc
     1021 gccttcggga cggactccgg gatctatccg cacgggatga acgcgaggaa cctcgcgttc
     1081 cacgtgcggt tcggccagac gccgatggag gcgatccgca gcgcgaccct cggcgcggcc
     1141 gaactcctgg gttgggacga ccgcgtgggg tctctgcgac cgggcctgtt cgccgacctg
     1201 atcgcggtcg acggcgatcc cctcgacgac gtgacggtgc tcgaggacgt tcccttcgtg
     1261 atgaagggcg gtcaggtcgt caagggctga ccggacccgg cagccggtgt ctgcgatgat
     1321 gacgcgcgtc cgcacgaggg agtcgagccg atgagggaag tccagccgct gtccgccgcc
     1381 gagatcgtcg agaagtcgaa gcgatacacg ctctacgact ggcaggccca gtcgaaggca
     1441 tcgccgatcg cggtggatcg agccgagggc gtgtacttct gggacgtcga cggcacgcgg
     1501 tacctcgact tcaactcgca gctgatgggc gtgaacatcg ggcacgggga taagcgcgtc
     1561 gccgacgcga tcgcgcggca ggcggagaag ctcccgtaca tctcgccgtt catggcgtac
     1621 gagacgcggg cacgcctggg agagaagctc gccacgctct ggccgggcga cctcgagaag
     1681 tccttcttca cgctcggcgg cgcggaggcg aacgagaacg cgatccggat cgcgaaggcg
     1741 ttcaccgggc gccagaagat cctggcgagg taccgctcgt accacggcgc cacttacgcc
     1801 acgatcaacc tgaccggcga cccacgccgc tgggcgaacg agaacccccc gatgcccggg
     1861 atcgtgcacg tcctcgaccc ctaccggggc accgcgcgcg gcaccgagga tgcggcgacg
     1921 gccctcgcga ccttggagga gacgatcgag ctcgagggac cccggacgat cgcggcgttc
     1981 atcctcgaga cggtcacggg gacgaacggg atcctgatcc cgcccgacgg ctacctcgag
     2041 ggagtccggg acctctgcaa ccgccacgag atcctgctga tcgcggacga ggtgatgtgc
     2101 gggttcgggc gcaccggcga gtggttcgcg gtgaaccact acggggtggt gcccgacctg
     2161 atgacggccg cgaagggcct gacctcgtcc tacctaccgc tcggggcggt cgcgatcaag
     2221 cccgagatcg ccgcacactt cgaggagaac gtcttctacg ggggactcac ctacaactcc
     2281 cacccgctct cgtgcgcggc ggcgatcgcg gcgatccagg tcctcgagga ggacgacctg
     2341 gtcgggaacg cgaagcggct ggacccggtg atgcgccggc atcacgagga gctgatggcg
     2401 aagcacccga gcgtgggccg cgtccgcaac atcggcctct tcgggatcat cgagctcgtc
     2461 aagaaccggg acacgatgga gccgctctcc ccctacaacg tcgtgaacga gacgatgcag
     2521 gccgtgaaca aggggctcct tcagcggggc ctgttcacga tgatccgctt caacgggatc
     2581 atgacgaacc cgccgctctg catcaccgag gagcagctgg acgacggctt cgcgatcatc
     2641 gacgaggtcc tcgacatcgc cgacgctgcg accggctgac gctgattcag gcgccgacag
     2701 ccgcccagcg gtcggcccac ttcgccaggc cgtcgatcac cttcgccagc tcgcgccccc
     2761 ggtccgtcag ggcgtactcg atcagcaccg gtgtcgaagg cgtgaccgct cgggtaagga
     2821 tgcccgccga ctccagatcc cggagccgat ccgacagcac ggtgtcggag atctccggga
     2881 tcgccgtgcg aacgaccgag tagcggcgag gtccgccgag gagcacccac acgatctggg
     2941 gcacccatcg ttgaccgacg agctgggccg cgtgctggaa gctctcgcag accgcgcgat
     3001 ccgtgtccat agccgaatct tagcggctaa gaccttcttt gaagctaagg aatactgagt
     3061 atctcgccgc ggttccgggt acgagaccga ggaaggcatc gacgtgtccg gagaggccgc
     3121 gtcgtccgag aggaaggagt ctctgcgatg gcagattcga cgaaggagat cggtggcctc
     3181 ccgttgccac ctccgggcaa gtacgagctc gacatcgccc acacggcggt ggagttcgtc
     3241 gcccgccaca tcttcacgaa ggttcgaggc cgcttcacgg acttctccgg atggatcgat
     3301 gtcgcggaga acccggaaga gtcctccgcc cacgtcgaga tcaagaccgc gagcatccaa
     3361 acgaacacgg agcagcggga cgagcacctg aagagcgacg acttcctgaa cgtcgagaag
     3421 tggccggtca tgacgttcgc cagcaccggg gtgcggcaca cgggcggagc cacgttcgag
     3481 ctggacggtg aactgacgat ccgcgacgtg acccgtgccg tgacgctcgt gggtgagtac
     3541 ctgggcaccg agacgaaccc ctacgggaag accgttctgg ccgcgacggc caagacgacg
     3601 ctcgagcgtg aggactggaa cgtgagctgg aacatggtcc tggagaccgg cggcttcctc
     3661 gtcagcaagg cgatcgatct cgagatcgag gtcgaagctt tgaagaccgg ctgatcacgt
     3721 catgggggcg tcggcaacgg tcccgacgcc cccgcttcgt acgagatgag ctcgaaggcg
     3781 cggtcgtagc ggacgacgac gtcgtccgcg acccagagat cgctcgtcca gccggtggcg
     3841 agcgccgtgt atccccatcg ggcggcgagg aacgtgcccg acggcgtctc gaccgactcg
     3901 cgcccgatca gctcgtaccg ctgacgcatc gggaccggtt cgagggtgac gggctcgagg
     3961 aagaggacgt cgatctcgct cgatccctcc aaccgttgcg ccgtgatcgc gttgaacgcc
     4021 ggtgagaggt agtccaggtg catctccggt ccccacgcca tctcgacctt gtgcccgtct
     4081 cggatccctt cgagtgagtc ccggcgcgcc tcgaggaggg tcgcgtgcga tcctgtctcg
     4141 atcctcgtcc ggacgatccg ccacgaggtg tcgaccacca cgtcgaccgt ctcccggtgg
     4201 ggtgtgggct catcgcgctc gatctcggag acataccgcc atcccatggg gccgggggcg
     4261 cagcggaagt cctcggttcc gacgacgtcg ccgctctgcc cgcgcaccga gtaccggccg
     4321 ctcggcacgt cagacctgtg gctggagccc gaggtcccgc gcgatcacca ggcggaggat
     4381 ctcgctcgtc ccctcgccga tctcgagcac cttcgcgtcg cggtacgcac gggcgaccgg
     4441 gaactcctcg gtgtagccgt agccgccgtg cacctggatc gcctcgcgtg cgacatcgac
     4501 ggcggtctcg ctcgcgcgga gcttcgcgat cgcggcctcc gtggtgtaag gccgtccctg
     4561 gtccttcagc caagcggctc ggtaggtcag cagccgcgac gcctcgaccg cggcacgcat
     4621 gtcggcgagc ttgaacgcga tcgcctcgta gttcccgatc ggccgtccga aggcctcacg
     4681 atccttcgcg tagctcaggc tggcatcgag gcacccctgg gcgaggccca ccgagagcgc
     4741 cgcgatcgag atccgtccgt cgtccagtgc ggccagacat tgggcgagac ccttgccgcg
     4801 ctccccgagg aggtggtcct cggggacccg gcatccctcg aaggacagct cgtgggtgtc
     4861 gctcgatctc caccccagct tccggtaggg ggggccgacc gagaaccctg gcgcgtcggc
     4921 cggcaccacg accgtggaga cgccgttggc tccggcatcg tcttccgtgc gacacgccac
     4981 caccacgaac ccggtgatct gggacccact gttcgtgatg aacgacttcg atccgtcgat
     5041 cacccagctc cggccgtcga gccgggccct cgtccggagc gcggcggcgt ccgagccgct
     5101 ccccggctcc gtgaggccga accccgcgag catctcgccc gcagcgatcg gggggagcca
     5161 ccgctgtcgc tgctccgtgc tcccgtagcg tgcgatcatc cccgcagcca gccccgacgc
     5221 gaccgatacc gtgaccgcga cgctcgcgtc ggccctgccc agttcctcga tcaccagcgc
     5281 ccaggtgacg gcgtctgctc cgagccctcc gtgctcctcg gggatcggga caccgcagat
     5341 cccgagctcg ccgagcttct tcacgatgtc gagggggagt tc
//
LOCUS       DASUVE010000306         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1470231, whole genome shotgun sequence.
ACCESSION   DASUVE010000306 DASUVE010000000
VERSION     DASUVE010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470231"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..812
                     /gene="pyrF"
                     /locus_tag="VF036_06585"
     CDS             129..812
                     /gene="pyrF"
                     /locus_tag="VF036_06585"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX6264931.1"
                     /translation="MIPRPTDPIVAALDVSTLGEADRLAEILAPHVGMLKVGLELAWS
                     AGPEAIRRVASHGPVFADCKLHDIPNTVERAAANVARLGVAMLNVHALGGEAMMRAGV
                     AGARRGAADAGVEPPLVVAVTVLSSHAGEGLASPASLAFEAKAAGLDGVVVSGEDVED
                     VRATCGEEFCLVVPGIRPAGSNGHDQVRVLTPAEAIERGADFLVIGRPVTEAPDPAGV
                     VRGIMRDLR"
     gene            1007..1318
                     /gene="mihF"
                     /locus_tag="VF036_06590"
     CDS             1007..1318
                     /gene="mihF"
                     /locus_tag="VF036_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEX6264932.1"
                     /translation="MALPTLTPEQRAQALAKAAEARKKRAELKGELKSGKRTLGDVLI
                     RSTEDTVGKMKVSAVLESLPGVGKVRAQKLMEELDISASRRVRGLGAKQRAQLLDRFK
                     K"
     gene            1325..1891
                     /gene="gmk"
                     /locus_tag="VF036_06595"
     CDS             1325..1891
                     /gene="gmk"
                     /locus_tag="VF036_06595"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX6264933.1"
                     /translation="MRTGKLFVLAGPSGVGKGTIVRDLVSRDPEGLSLSVSVTTRAPR
                     PAEVDGVDYFFVDDDAFDRMIRAGELLEWAEIVGHRSGTPRGFVEDRLAAGRDVVLEI
                     DVVGASQIRDRVPGSVLIFVEPPSMEELERRLRGRGTETEDRIRLRLETAGWELEQRN
                     WFDHAIVNDDLERASSQVAAIIEASRSA"
BASE COUNT          338 a          686 c          756 g          298 t
ORIGIN      
        1 gacccggccg cccccgtcga gctcgctcgg ggcatcgcgg ggtacctgaa ggagaagggc
       61 ctcgggacac cgaacgatct ccgtgctcgg ctgcgggtcc ccgtcgggtt ccgggaaccc
      121 gaggagccat gatcccgcgg cccaccgatc cgatcgtcgc ggcactcgac gtctcgacgc
      181 tcggggaagc ggaccggctg gcggagatcc tcgcccccca cgtgggcatg ctcaaggtgg
      241 ggctcgagct cgcatggtcg gccgggccgg aggcgatccg gcgggtcgct tcgcacgggc
      301 cggtcttcgc cgactgcaag cttcacgaca tccccaacac cgtcgaacgc gcggcggcca
      361 acgtcgctcg gctcggggtc gcgatgctga acgtccatgc gctcggaggc gaggcgatga
      421 tgcgagccgg cgtcgcaggg gcgcgacggg gcgcagccga cgcgggtgtc gaacccccgc
      481 tggtcgtcgc ggtcaccgtc ctgtcgagcc acgcggggga ggggctcgcg agtccggcat
      541 cgctcgcatt cgaggcgaag gcggccgggc tggacggggt cgtggtctcc ggggaggacg
      601 tggaggacgt gcgcgcgacg tgcggcgagg agttctgcct cgtcgtcccg gggatccgac
      661 cggcgggatc caacgggcac gaccaggtac gggtcctcac gcccgccgaa gcgatcgagc
      721 ggggcgccga cttcctcgtg atcggtcgcc cggtgacgga agctcccgac cctgcgggcg
      781 tcgtcagggg gatcatgcgc gatctccgct gaccgttgcg gcgtcgggga aaccgcaggc
      841 cacatgcgaa acttcgcttg ccacgccgac acgagccgtg catatactcg cgccctcgca
      901 ccgtccgacc cggccgtgga tcgtggcctt gccttccatc gaggccgacg atcacggcca
      961 acgacgacgg ccacgtcgtc ggccaccgaa cagaaggagc atgggcatgg cactcccgac
     1021 cctcaccccg gaacaacgcg cgcaggccct cgcgaaggct gcggaggcgc ggaagaagcg
     1081 cgcggagctc aagggagagc tcaagtccgg gaagcggacg ctgggagacg tgctgatccg
     1141 gtcgacggag gacaccgtgg ggaagatgaa agtctcggcg gtcctcgagt cgttgcccgg
     1201 tgtcggcaag gtgcgcgcac agaagctgat ggaagagctc gacatctcgg cgagccgtcg
     1261 cgttcgggga ctcggtgcga agcaacgcgc ccagctcctc gaccggttca agaagtaacg
     1321 ggccatccgc accgggaagc tcttcgtcct cgccggtcca tcgggcgtgg gcaagggcac
     1381 catcgttcgc gacctcgtct cgcgtgaccc cgagggcctt tcgctgtcag tctcggtgac
     1441 gacccgagcg ccgcggcccg cggaggtcga cggggtggac tacttcttcg tcgacgacga
     1501 cgccttcgat cggatgatcc gggcggggga gctcctcgag tgggccgaga tcgtcggaca
     1561 ccggtcgggg acgcctcgcg ggttcgtcga ggaccgtctc gccgccggtc gggacgtggt
     1621 cctcgagatc gacgtcgtcg gcgcctccca gatccgcgac cgggtccccg gctccgtgtt
     1681 gatcttcgtc gagcccccgt cgatggagga gctggagcgg cgcctgcgtg gtcggggcac
     1741 cgagaccgag gaccggatcc ggctccgcct cgagacggcg gggtgggagc tggagcagcg
     1801 taattggttc gatcacgcga tcgtgaacga cgatctggaa cgagcatcgt cgcaggtcgc
     1861 tgctatcatc gaagcgtccc gctccgcctg aggaggccgc ggggactccc gccgaagcgt
     1921 gaagccaccg aagcgcgaag cgaccgaagc gcgaagcgac cgaagtgtga agccaccgaa
     1981 gtgtgaagcc accgaagtgt gaagaaggaa ggccttcctg tgatcgagcc caagatcgac
     2041 gacctgctcg ccgccgtgga ttcgaagtac tccctggt
//
LOCUS       DASUVE010000307         2978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_718486, whole genome shotgun sequence.
ACCESSION   DASUVE010000307 DASUVE010000000
VERSION     DASUVE010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718486"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1219
                     /locus_tag="VF036_06600"
     CDS             281..1219
                     /locus_tag="VF036_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6264934.1"
                     /translation="MTATLVCSGLRKSFGERVAVDGVEFSIAAGETYGLLGPNGAGKT
                     TTISMVCGLLTRDAGEILLEGRAFDTTSIDAKASIGLVPQEIAIYPDLSANENLAFFG
                     RLYGLHDPELSERVAEVLETVGLQDRANDRTSEFSGGMKRRLNIGLGLLHRPRLLVLD
                     EPTVGVDPQSRNAILDSVEQLGAGGLSVLYTTHYMEEAERLCDRVGILDEGRLLAEGT
                     RRELVSSIGAHDRVRMIAPGISEPVLVGLRGVAGVVEVSARDAQVDLLVDDARGSLGP
                     IVQTVTSDGGTVSSVEVQEPDLEAVFLSLTGKALRD"
     gene            1225..2373
                     /locus_tag="VF036_06605"
     CDS             1225..2373
                     /locus_tag="VF036_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6264935.1"
                     /translation="MGTPQGRASRTIAEKDLRQRIRDRTALLVGVVLPFALASILALT
                     LGGAAEGDLDLDGGPVADRFEQTLRAADVGTVHPLGDRDVAVRRAEEGALDAVFVLPV
                     GFSARSTSGGGGSIEVIGSPDSDLGPLVATSIARSFAARLDGFAIAVATASETPEDGP
                     AIADAAAQLPPAASLDISVTADEGVTPSTFTAIGMAVFFLFFTVEFGIRSVIEEREQG
                     TLARLLAAPIRPASVLIGKGLASFVVGVLSTGLLVVATTLLLDAAWGPLIGVALLVVT
                     GVATAVAVTGFVATFATTSNQAGNYASVVAVVGGLLGGTFFPISQAPGLLASARFLSP
                     QGWLMEGFTELSTGGGLVGSLPALAGTLVIGVAFGTLAWVRARRMLSP"
     gene            2370..>2978
                     /locus_tag="VF036_06610"
     CDS             2370..>2978
                     /locus_tag="VF036_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6264936.1"
                     /translation="MSSARKALAIAAVNVRRLTKDRTGLFFVVIFPFLLIFVLGATFG
                     SGFTPRVGVVAARAPLAGEIVQRLRSVDGIEVRTFVSADALGEAVLLGDVQGGFVIPP
                     SYDVDIRDGRPVEIPWVSLPTGAGGDLSLIVGSVIDQQSSLVNAARLAHERGAADSFD
                     EALTRAREESGTIPIVAVRTSTAGNPEGSGLSTAAAQELTLFV"
BASE COUNT          444 a          997 c         1019 g          518 t
ORIGIN      
        1 ccctcggcga ccccgagcac gaacagacag atcaggcaga cctcggcgtc cagagccgat
       61 ctctcgaccc ccggacgagt cgaacgtgaa gtgcggcgag gagcaccgga aggagcgaga
      121 acgcgaacag tcgcacggcg accggtgtcc acccggagtt ctccatctcg tggccaggct
      181 cgcttcctga tgccgaacat gcaaccaggc tcctgagaag acctctgtca ctccagacac
      241 ggtgagcacg ccctggaggt cgtccgctag gctgcggcga atgaccgcga cgctggtgtg
      301 tagcgggctc cgaaagtcgt tcggcgagcg ggtagcggtc gatggcgtgg agttctccat
      361 cgccgccggc gagacctatg ggctactcgg gcctaacggc gccggcaaga ccacgaccat
      421 ctcgatggtg tgcggtctgc tgacacgaga cgccggcgag atcttgctcg aaggccgggc
      481 cttcgatacg acgagcatcg atgcgaaggc ctccatcggt ctcgtccccc aggagatcgc
      541 gatctatccg gatctgtcgg cgaacgagaa cctcgcgttc ttcggccgcc tgtacgggct
      601 tcacgatccc gagctcagcg agcgggtcgc cgaggtcctc gagaccgtcg ggcttcagga
      661 ccgggccaac gatcgaacat cggagttctc aggcgggatg aagcgccggc tgaacatcgg
      721 acttggactc ctccatcggc cgcgtctcct cgtcctggac gagccgaccg tcggcgtgga
      781 cccgcagagt cgcaatgcca tcctcgactc cgtcgagcag ctcggcgcgg gtggcctctc
      841 ggtcctgtac acgacgcact acatggagga ggcggagcgc ctctgtgacc gggtggggat
      901 cctcgacgag gggcgactgc tcgccgaggg cacccggcga gagctcgtct cgagcatcgg
      961 ggcgcacgat cgcgtccgga tgatcgctcc ggggatctcc gagccagtgc tcgttggcct
     1021 gcgcggcgtt gccggcgtgg tcgaggtctc tgcacgagac gcccaggtcg accttctcgt
     1081 cgatgacgca aggggttcac tcgggccgat cgttcagacc gtcaccagcg atggcggcac
     1141 cgtttcctcc gtggaggttc aggagcccga tctcgaggcg gtgttcctgt cgctcacggg
     1201 caaggcgctt cgcgactagc gccgatgggc acaccccagg gccgggcgtc tcgaacgatc
     1261 gcggagaagg accttcggca gcggatacgg gaccggaccg ccctcctggt gggcgtggtc
     1321 ctcccgttcg cgctggcgtc gatcctcgcc ctcacgctgg gcggcgccgc cgagggcgac
     1381 ctcgatctcg acggagggcc cgtcgccgac cggttcgagc agacgctccg agcagccgac
     1441 gtcgggaccg tgcaccctct cggcgatcga gatgtggcag tacgacgagc cgaggagggc
     1501 gccctcgacg ccgtgttcgt gcttccggtt ggcttctcgg ctcggtccac atccggtggt
     1561 ggaggatcga tcgaggtgat cggctcgccc gactccgatc tcgggccgct ggtcgccacc
     1621 tcgatcgctc gctccttcgc cgcgcgactc gacggattcg cgatcgccgt tgcgacggca
     1681 agcgagactc ccgaggacgg gccggccatc gccgatgcgg cggcgcagtt gccgcccgct
     1741 gcctctctgg acatcagtgt gaccgccgac gaaggcgtca ctccgtcgac cttcacggcg
     1801 atcggcatgg cggtgttctt cctcttcttc acggttgagt tcggcatccg cagcgtgatc
     1861 gaggagcgtg agcagggcac actcgcgcga ctgctcgccg ccccgatccg tccggcctcc
     1921 gtgttgatcg gcaagggtct cgccagcttc gtggtgggcg ttctctcgac cggcctgctc
     1981 gtggtggcca ccaccctgtt gctcgatgcg gcgtggggtc cgttgatcgg cgtcgcgctg
     2041 ctggtcgtga cgggcgtggc cacggcagtg gcggtcaccg gcttcgtcgc gaccttcgcc
     2101 accacgtcga accaagccgg caactatgca tcggtggttg ccgtcgtcgg cgggctcctc
     2161 ggaggaacct tcttcccgat ctcacaggca ccgggactcc tcgcgtccgc tcgattcctc
     2221 tccccgcagg ggtggctcat ggaaggattc accgagctct cgaccggcgg cggactcgtc
     2281 ggttcactcc ctgccctggc cggcacgctc gtgatcggag tggcgttcgg aaccctcgcg
     2341 tgggtccgag cccgaaggat gctgagccca tgagctccgc ccgcaaggcg ctcgcgatcg
     2401 cggcggtcaa cgttcggcgg ctgacgaagg accgcaccgg cttgttcttc gtggtgatat
     2461 tcccgttcct gctgatcttc gtcctaggag ccaccttcgg ctccggattc acaccgcgcg
     2521 tgggcgtcgt ggcggcgcgg gcgcccctcg ccggcgagat cgtgcagcgt ctgcgctcgg
     2581 tggacgggat cgaggtccgg acgttcgtga gcgctgatgc cttgggcgag gcggttctgc
     2641 tcggcgatgt ccaaggcggc ttcgtgattc cccccagcta cgacgtcgac atccgtgacg
     2701 gacgaccggt cgagatcccg tgggtgtctc tgcccaccgg agcaggcggt gacctctcgc
     2761 tcatcgtcgg gtcggtgatc gaccagcaga gctcgctcgt gaacgcggcc cgcttggcgc
     2821 atgagcgggg ggcagcggac tcgttcgacg aggccctgac gagagcccgg gaggagagcg
     2881 gaacgatccc gatcgttgcg gttcggacat cgacggcagg gaacccggaa ggaagcggac
     2941 tcagcacggc agccgcccag gaactgacgc tcttcgtg
//
LOCUS       DASUVE010000308         4023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1283309, whole genome shotgun sequence.
ACCESSION   DASUVE010000308 DASUVE010000000
VERSION     DASUVE010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283309"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /gene="infC"
                     /locus_tag="VF036_06615"
     CDS             complement(<1..543)
                     /gene="infC"
                     /locus_tag="VF036_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX6264937.1"
                     /translation="MNDRIRSPQVRLVGADGEQIGVVATQEAVRRARELDLDLVEVAP
                     QANPPVCRIMDYGKFKYERDIRQKEARKKQARTELKEIKFRPKIDAHDYGTKKGHVER
                     FLRGGHKVKVTIMFRGREMTHTELGRKILDRLVGDLGEMVTVESMPKQEGRNMIMVIS
                     PNKRYMEQLARARARESAPVV"
     gene            complement(688..1038)
                     /locus_tag="VF036_06620"
     CDS             complement(688..1038)
                     /locus_tag="VF036_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HEX6264938.1"
                     /translation="MIVEKPWGRVVTYALNQPSSVRVITVEPGQETSVHYHRMRDETW
                     VVLDPGLSIEIGDRTVDAGPGEEFVIPAEQAHRIRCNGSGPGRILEVAYGYTTEDDTQ
                     RLQDAYGRSLEPDW"
     gene            complement(1035..1538)
                     /gene="ribH"
                     /locus_tag="VF036_06625"
     CDS             complement(1035..1538)
                     /gene="ribH"
                     /locus_tag="VF036_06625"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX6264939.1"
                     /translation="MTVSRGAPEGAGRRVGVVAARFHEHVTAKLLEGALGGLRARGVA
                     DDAVDVAWVPGAFEIPLVARRLASTGAYDALICLGAVVRGETAHFDLVAGEAARGIAD
                     VSRTTGIPVIFEVLATESLADAEARAGGPRGNKGWDAAEAALEMADLLDRLPDANERE
                     QREGSTR"
     gene            complement(1535..2803)
                     /locus_tag="VF036_06630"
     CDS             complement(1535..2803)
                     /locus_tag="VF036_06630"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX6264940.1"
                     /translation="MSPLSDTSVFASIPEAIDEIRQGRMVIVVDDADRENEGDLIMAA
                     ERVTPEAIAFIVRHTSGVICMPVIGERLDELEIPLMVAANTDSRRTAFTVSVDARMGV
                     TTGISAADRARTIKAIIDPESEPDDLSRPGHVFPLRYREGGVLKRAGHTEAAVDLARL
                     AGLFPAGVLCETVNEDGTMARLPDLERFARAHDLKIISIADLIEHRRQNEVLVTKVAE
                     ANIPTRHGEFRSIAYESVTDARTHVALVMGDVGDGQNVLTRVHSECLTGDVFGSLRCD
                     CGGQLDRAMELIGREGRGVILYVRGHEGRAIGITHKLRAYALQDRGRDTVEANLELGF
                     PADRRDYGIGAQILYDVGVRSMRLLTNNPEKRAGLEGHGLAINERLPLQTAPTPQNVG
                     YLRAKREKLGHLLDLPEMDGPAIDEVEARA"
     gene            complement(2800..3429)
                     /locus_tag="VF036_06635"
     CDS             complement(2800..3429)
                     /locus_tag="VF036_06635"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX6264941.1"
                     /translation="MFTGIVEELGTVVSRDGERLVVRGSVTSSDAAIGDSLAIDGVCL
                     TVVELQGEQLSFDLSEETLARTNLGSLGPGDAVNLERPATLVSRLGGHLVQGHVDGVA
                     EVTAVRPEGEDGVRLTVRLPGELLRHVVEKGSITLDGVSLTVAALDGEEIEIALIPHT
                     LHATTLGSVREGDRVNLEVDIVAKYVARNLEYLTTDGATVVRPRKERTA"
     gene            complement(3410..>4023)
                     /locus_tag="VF036_06640"
     CDS             complement(3410..>4023)
                     /locus_tag="VF036_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HEX6264942.1"
                     /translation="RDGSSMWLTGEKARADVQRLRAWSDAVVVGSGTALADDPRLTVR
                     DAAYASARPPLRVIVDPSGRVTGEGRAFDEAAPTLVATTELSSPSRRQEWLDAGADVA
                     VVDRDESGVSLSALVEILGKRDIQGVLVEGGPTLAWSAIRDGVVDQIVLYLAPILIGG
                     RDAPGWLAGAGAPTLGKASQLDIASVERLDRDLKVVADVHRDR"
BASE COUNT          590 a         1459 c         1307 g          667 t
ORIGIN      
        1 gacgacgggt gcggattcgc gagctcgcgc gcgggcgagc tgttccatgt accgcttgtt
       61 gggcgagatc accatgatca tgttccgccc ctcctgcttc ggcatcgact ccacggtcac
      121 catctcaccc aggtctccca ccagccggtc caggatcttg cgcccgagct ccgtgtgggt
      181 catctctcga ccccggaaca tgatggtgac cttcaccttg tgcccgcccc ggaggaaccg
      241 ttcgacgtgg cccttcttcg tgccgtagtc atgcgcgtcg atcttcgggc ggaacttgat
      301 ctccttgagc tcggtgcggg cctgtttctt ccgagcttcc ttctgacgga tgtctcgctc
      361 gtacttgaac ttgccgtagt ccatgatccg acagaccggc gggttcgcct ggggtgcaac
      421 ctcgacgagg tccaggtcga gctcgcgtgc cctgcggacg gcttcctgtg tcgcgaccac
      481 cccgatctgc tcgccgtcgg cgccgacgag gcgcacctgg ggagatcgga tcctgtcgtt
      541 caagcgtggc tctgtcgaga ccccgcatca ctcccttcga aaacgaagca ccgggccagc
      601 cggtgcttgt ggcggatcag acggaacacg tgcgcagagt atagggccaa aatcatgggg
      661 atcgcctctg acccctatcg atgcagctca ccagtccggc tcgagtgagc gtccgtacgc
      721 atcctggagg cgctgcgtgt cgtcctcggt cgtatagccg tacgcgacct caaggatccg
      781 tcccggaccg gagccgttgc agcggatgcg atgcgcctgc tcggcgggga tcacgaactc
      841 ctcgcccgga ccggcgtcga cggtccgatc accgatctcg atcgagaggc ccgggtcgag
      901 gaccacccac gtctcatcgc gcatccggtg gtagtggacg ctcgtctcct gaccgggctc
      961 caccgtgatg acccggaccg acgacggctg gttgagggcg tacgtgacca ctctccccca
     1021 cggcttctcc acgatcatcg cgtgctccct tccctctgtt cccgttcgtt cgcgtcgggc
     1081 aaccggtcga gcagatcggc catctccagc gcggcctccg ctgcgtccca ccccttgttg
     1141 ccgcgggggc cgcccgctcg ggcctcggcg tcggccaggg actcggtcgc caggacctcg
     1201 aagatgacgg ggatcccggt cgtcctgctg acgtcggcga tcccgcgcgc cgcctcgccg
     1261 gccacgagat cgaagtgggc cgtttcgccc cggaccacgg cgccgagaca gatcagtgcg
     1321 tcgtaggcgc cggtggaggc cagccgtcgg gccacgagcg ggatctcgaa ggcacccgga
     1381 acccaggcga catcgaccgc atcgtcggcg acgccgcggg cacggagccc gccgagcgca
     1441 ccctccaaga gcttcgcggt gacgtgctcg tggaaccggg cggcgacgac gccgacacgg
     1501 cgcccggcgc cctccggggc gcctctcgat accgtcatgc tcgcgcttcc acctcgtcga
     1561 tcgccggtcc gtccatctcg gggagatcca ggagatgacc gagcttctct cgcttggccc
     1621 ggaggtagcc gacgttctgc ggcgtcggag ccgtctgcag cggcagccgc tcgttgatcg
     1681 cgaggccatg tccctcgagg cccgcccgct tctccgggtt gttcgtgagc agtcgcatgg
     1741 atcggacccc gacgtcgtac aggatctggg cgccgatgcc gtagtcgcgt cgatcggccg
     1801 ggaacccgag ctcgaggttc gcctccaccg tatctcgacc gcgatcctgg agggcgtagg
     1861 cgcggagctt gtgcgtgatg ccgatcgcgc ggccctcgtg cccccggacg tagaggatca
     1921 cgccgcgccc ttcacgaccg atcaactcca tcgcccggtc gagttgcccc ccgcagtcac
     1981 agcggagcga tccgaacacg tcgccggtga ggcactcgga gtgcacccgg gtcaggacgt
     2041 tctggccgtc accgacatcg cccatcacca gggcgacgtg cgtccgcgcg tcggtcacgc
     2101 tctcgtacgc gatcgaccgg aactccccgt ggcgcgtggg gatgttcgcc tcggccacct
     2161 tggtcacgag gacctcgttc tggcggcggt gctcgatcag gtcggcgatc gagatgatct
     2221 tcagatcgtg cgcacgggcg aatcgctcca ggtccgggag ccgggccatc gtgccgtcct
     2281 cgttgactgt ctcgcacagg acgccggccg ggaagaggcc ggccagccgc gcgaggtcga
     2341 cggcggcctc cgtgtgtccc gcccgcttga ggacaccgcc ttcccgatag cggaggggga
     2401 agacgtgacc aggccgcgac aggtcgtccg gctcggattc gggatcgatg atcgccttga
     2461 tcgtgcgtgc gcgatccgcg gccgagatgc ccgtcgtgac gcccatccgc gcgtcgacgg
     2521 acacggtgaa cgcggtccgc cgggaatcgg tgttcgcggc caccatcagc gggatctcga
     2581 gctcgtcgag tcgttcgccg atcaccggca tgcagatcac cccactggtg tggcggacga
     2641 tgaacgcgat cgcctccggc gtcacgcgct ccgcggccat gatcaggtcg ccctcgttct
     2701 cccgatcagc gtcgtcgacg acgatcacca tccggccctg gcggatctcg tcgatcgcct
     2761 ccgggatcga ggcgaagacg ctcgtgtcgc tcagcgggct catgccgttc gttcctttct
     2821 cggtcggacg acggtcgcgc cgtcggtcgt gaggtactcg aggttccggg cgacgtactt
     2881 ggccacgatg tcgacctcca gattcacccg gtcgccctcg cggaccgagc cgagcgtggt
     2941 ggcgtggagg gtgtgcggga tcaacgcgat ctcgatctcc tcgccgtcga gggccgccac
     3001 ggtcaggctc acaccgtcga gcgtgatcga ccccttctcc acgacgtggc ggagcagctc
     3061 tccggggagt cgcaccgtga gtcggacgcc gtcctcgccc tcgggtcgga cggcggtcac
     3121 ctcggcgacg ccatccacgt gtccctgcac gagatggcct ccgaggcgag agacgagcgt
     3181 tgccggtcgt tccaggttca ccgcgtcccc gggaccgagc gagccgaggt tcgtccgcgc
     3241 caacgtctcc tccgagagat cgaaggagag ctgctccccc tggagctcga cgaccgtgag
     3301 gcagaccccg tcaatcgcga gcgaatctcc gatcgccgcg tccgaggacg tgaccgaacc
     3361 gcgcacgacc aggcgctccc cgtcgcgcga gaccacggtt ccgagctcct caacgatccc
     3421 ggtgaacatc ggccaccacc ttcaggtccc ggtcgagccg ttccacggac gcgatgtcga
     3481 gctgcgacgc cttcccgagc gtgggcgcgc ccgccccggc cagccacccc ggcgcgtcgc
     3541 ggccgccgat caagatcggc gcgaggtaca ggacgatctg atccacgacg ccgtcgcgga
     3601 tcgcgctcca ggcgagcgtc gggccgccct ccaccagcac accctggatg tcccgcttgc
     3661 cgaggatctc caccagcgcc gacagcgaca caccgctctc gtcgcggtcc accaccgcca
     3721 cgtccgcacc tgcgtcgagc cattcctggc gcctcgacgg cgatgagagc tcggtcgtcg
     3781 ccaccagcgt cggcgcggcc tcgtcgaagg cccgcccctc accggtgacc cggccggacg
     3841 gatccacgat gacccggagc gggggacgcg ccgacgcgta cgcggcgtct cgcaccgtca
     3901 accggggatc gtcggcgagg gcggtccccg agcccaccac gacggcatcg gaccacgcgc
     3961 gcaaccgctg gacgtccgct ctggccttct cgccggtgag ccacatcgac gatccgtcac
     4021 gag
//
LOCUS       DASUVE010000309         4887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_905244, whole genome shotgun sequence.
ACCESSION   DASUVE010000309 DASUVE010000000
VERSION     DASUVE010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905244"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..2042
                     /locus_tag="VF036_06645"
     CDS             21..2042
                     /locus_tag="VF036_06645"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HEX6264943.1"
                     /translation="MGRRATVAGSPVAVPARTIRRRRSRRWGIRVVLIGLAVLVVVAG
                     LSWLPARAAADRLEGGRAAMVRGLELLSAGNANGAQRAFALASEEFLAASREAEHPLI
                     RLGGSVPLAGRSYEALGTLARIGGRTAAAGARVTTALAEIPGGLGSLAPRDGRIPVEE
                     IGSLLPSVSAARAELEAASRESQAMPTSYLVGPVESAADLVRDEVESALRTTRSAEAL
                     LRALPSFVGAEGPRRYFVAAQSPAELRGTGGFIGSYAILTAREGRLKLGPFREISVLR
                     NVSAEAAPAPSDAFREIYDRFGGAGFWRNLNMTPDVPTAASMIEDLYERVEGTPLDGT
                     IFVNPQVLSELLEATGPVHAPIVDRTLTAEGIVDFLANGAYEEFGFRAGIRKRVLGIA
                     ATSVFRTFLQRPDPDTAVSSMIDAASGGHLTLHAADPEIQSAFRSAGVAGELGTPRGD
                     FFGLFTSDASGTKVDYYVRREIRYEVTLGANGSASVEASIVFHNDAPAGAAPSYVLGP
                     YPGTGLTVGEELSFASVYCASGCEMTGATEDGEPAGMEVHREHGLRSLHRYIRVEPQA
                     SRTLDLSLRIRRAWEGDDAAGTYRLHLQGQATIRPTDATVLVRVPEGMRIVGTTDSMR
                     VIGDEACGAALSAPSSTSRCDSAGLWPDGSGEPSGAAEAHAATVGRAGS"
     gene            2110..3447
                     /locus_tag="VF036_06650"
     CDS             2110..3447
                     /locus_tag="VF036_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX6264944.1"
                     /translation="MRTDTAQDVWLRPDPLEHLAERIRTKQAKVAVVGLGYVGLPLLV
                     SVGRSGFSVMGFDVDRGRVESLRAGHSFLVDVTDTEVVSLDGAEFREDPAALADADVV
                     LICVPTPLTDHTPDLAMVREAVQTVGRHLAPGRLVVLESTTYPGTTEEVVAPILEGAS
                     GLTAGRDFALAYSPERIDPGQDLHPLDETPKIVAGLTDRCRDLASSFYSLVIQLVVVV
                     SSPRDAEMAKLIENTFRQVNIALVNELAVLARDLQVDIWEALRAASTKPFGYMPFWPG
                     PGVGGHCIAIDPSYLSWRAGQQTGYHIGFIEHANEVNNRMPAYVVSRIGDALNDAGKP
                     VHGSRLLAVGVTYKPGVDDQRGSPSLMVMERLAAKGAIVAYHDPFVPSLQLAGTSLES
                     QELTEELLASQDCVMILTAHPSVDYPFVADVAPLVFDARGATMGIDAPNVIRL"
     gene            3570..3776
                     /locus_tag="VF036_06655"
     CDS             3570..3776
                     /locus_tag="VF036_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264945.1"
                     /translation="MDPDRPDEPLVAFLATKGDQTFSWDVSPTLAASLRTYFESEGWE
                     VSLSTGGGPRRRLLPRVVRGDIQR"
     gene            4352..4726
                     /locus_tag="VF036_06660"
     CDS             4352..4726
                     /locus_tag="VF036_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264946.1"
                     /translation="MAEQTLDMFRSGFVPRSGKKQTEMAASYEDGDARLRLSSAQWVV
                     ALRPVIRGDPIVVIATRCRTSPNRVRRWLKSPISRRAVAAEIAQPTEERFRLSITLRG
                     VQEANSLRRERAAGKELVTGDE"
     gene            4719..4871
                     /locus_tag="VF036_06665"
     CDS             4719..4871
                     /locus_tag="VF036_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264947.1"
                     /translation="MSKEKGQEPEHTRARSDLDQTDRRGLSDKQQLAIDLLLVGTKYQ
                     DVAKAV"
BASE COUNT          751 a         1645 c         1692 g          799 t
ORIGIN      
        1 cgggccagat gtggggctag gtgggccgga gggcgaccgt ggccggcagc ccggtcgccg
       61 tgcccgctcg gacgatccgc cgtcggaggt cgcgccggtg gggaatacgc gtcgtcctga
      121 tcggcctcgc cgtgctggtc gttgtcgcag ggctctcgtg gctcccggcg cgcgccgccg
      181 ctgatcgcct ggagggggga cgtgctgcga tggtgcgtgg cctcgaactc ctgtcggccg
      241 gcaacgccaa cggtgcgcag cgcgccttcg ccctcgcctc cgaggagttc ctggcggcga
      301 gccgggaagc ggagcacccg ctcatcaggc tgggcggatc cgtgccgctc gcgggaagga
      361 gctatgaggc gctcgggacg ctggcgagga tcgggggacg tacggcggcc gcaggtgcgc
      421 gggtcacgac cgcgctggcg gagatccccg gcgggctcgg ctccctcgct ccgcgggacg
      481 gacggatccc ggtcgaggag atcgggtcgc tgctcccctc ggtgtcggcg gcacgggcgg
      541 agctcgaggc cgccagtcgc gagagccaag cgatgccgac gtcatacctg gtcgggcccg
      601 tcgaatcggc cgccgacctc gttcgtgacg aggtggaatc agctctgagg acgacgcgct
      661 cggcggaggc gctcctgcgt gcactgccgt cgttcgtcgg cgctgagggg ccccgccgat
      721 acttcgtggc ggcgcagtcc cccgcggagc tccgagggac gggaggattc atcggcagct
      781 acgcgatctt gactgcgagg gagggacgtc tgaagctcgg cccgttccgc gagatctcgg
      841 tcctgcgcaa cgtgtcggcc gaagccgccc cggcgccgtc ggacgccttc cgcgagatct
      901 acgatcgctt cggcggggcc ggcttctggc ggaacctgaa catgaccccg gacgtcccga
      961 ccgcagcctc gatgatcgag gacctctacg agcgtgtgga ggggacccct ttggacggga
     1021 cgatcttcgt gaacccacag gtgctctccg agctcctgga ggcgacgggt cccgtgcacg
     1081 ctcctatcgt cgaccggacc ctcaccgccg aggggatcgt cgacttcctc gcgaacggcg
     1141 cgtacgagga gttcgggttc cgggcaggca tccgcaagcg cgtcctcggg atcgcggcga
     1201 cgagcgtgtt caggacgttc ctccagcgtc ccgaccccga caccgcggtc tcatcgatga
     1261 tcgacgcggc ttcgggggga cacctcacgc tccacgccgc cgaccccgag atccagtccg
     1321 cgttcagatc ggcgggggtt gccggcgagc tcgggacacc ccgtggggac ttcttcgggc
     1381 tcttcacatc cgacgcctcc ggcaccaagg tcgactacta cgtgcggcgc gagatccgct
     1441 acgaggtgac gctgggtgcg aacgggtcgg cgtccgtcga ggcgtcgatc gtattccaca
     1501 acgacgctcc ggcgggcgcc gctccgagct acgtgctcgg cccctacccc gggaccggac
     1561 tcacggtggg cgaggagctg tccttcgcct cggtctactg cgcctccggt tgcgagatga
     1621 ccggcgccac cgaggacggc gagcccgccg ggatggaggt gcaccgggag catgggttgc
     1681 gttccctgca ccgctacatc cgggtggagc cgcaggcgtc gcgcaccctg gacctgtcgc
     1741 tgcggatccg ccgagcgtgg gaaggggacg acgccgcggg cacgtaccgg ctgcacctgc
     1801 aggggcaggc cacgatccgg ccgacggacg caacggtcct cgtgagggtg ccggagggga
     1861 tgcggatcgt ggggacgacc gactcgatgc gcgtgatcgg cgacgaggcg tgtggcgcgg
     1921 cactctcggc tccgagctcg acctcgaggt gcgattccgc aggcctctgg ccggacggat
     1981 ctggcgaacc ctctggggcg gctgaggctc acgccgcgac cgtaggacgg gcgggcagtt
     2041 gactcgcggg tcacctggga tagcgtgtgt gcgaccgagg gccccgtcat ggcaccaccg
     2101 ggaggagccg tgaggaccga caccgctcag gacgtctggc tccgccccga ccccctggaa
     2161 cacctcgccg agcggatccg tacgaagcaa gccaaagttg ctgtcgtcgg tctcggctac
     2221 gtcggcctcc cgctcctcgt gtccgtgggc aggagcggct tctccgtcat gggtttcgat
     2281 gtcgacaggg ggcgcgtgga gagcctgcga gcggggcact cgttcctcgt cgatgtcacc
     2341 gacacggagg tcgtctctct cgacggcgcg gagttccgcg aggaccctgc cgctctggcg
     2401 gacgcggacg tcgtcctgat ctgcgtgccc acgccgctga ccgaccacac gcccgatctg
     2461 gcgatggtgc gggaagcggt gcagaccgtg ggtcgccacc tcgcccctgg gcgcctggtc
     2521 gtcctggaat ccacgaccta tccggggacc accgaggaag tcgtggcccc gatcctcgag
     2581 ggggcttcgg gactcacggc gggcagggac ttcgcgctcg cgtactctcc ggagcgcatc
     2641 gatccgggac aggacctcca tcccctggat gagacaccga agatcgtcgc cggtctcacg
     2701 gatcgctgcc gcgacctggc gtcctccttc tactccctgg tgatccagct cgtggtcgtc
     2761 gtctcctcac cgcgagacgc ggagatggcg aagctgatcg agaacacctt ccgccaggtg
     2821 aacatcgcgc tggtgaacga gctcgcggtc ctggcgcgtg acctgcaggt cgacatctgg
     2881 gaggcgctcc gagcagccag cacgaagccc ttcggctaca tgcccttctg gcccggaccg
     2941 ggtgtgggcg ggcactgcat cgcgatcgat ccctcgtatc tgtcgtggcg tgccggccag
     3001 cagacgggct accacatcgg gttcatcgag cacgccaacg aggtcaacaa ccggatgccc
     3061 gcctacgtcg tgagcaggat cggcgacgcc ctgaacgacg ccgggaagcc cgttcacggc
     3121 tcgcgcctgc tcgcggtcgg cgtcacgtac aagccggggg tcgacgacca gcgtgggtcc
     3181 ccgtcgctca tggtgatgga gcggctcgcc gcgaagggag ccatcgtcgc ctatcacgac
     3241 ccgttcgtcc caagcctgca gctggccggg acttccctcg agtcacagga gctcacggag
     3301 gagctgctcg catcccagga ctgcgtcatg atcctcaccg cgcacccgtc ggtggactac
     3361 ccgttcgtcg ccgatgtggc tccgctggtg ttcgacgctc ggggcgcgac gatgggcatc
     3421 gacgcaccga acgtgatccg cctctagcga gaccctggtc gtgggtgcgt tcgggccttt
     3481 gagcatatcc agggccgtcc cgggtggagt ccacgtcgtc agcaggtcgt cctctcaagc
     3541 gaatccccca tcctgccgac atgctgtagg tggatccaga taggccggat gagccgctcg
     3601 tcgcgttcct cgcgacgaaa ggcgatcaga cgttctcttg ggacgtgagc cccacgcttg
     3661 ccgcgtcgct ccgcacctac ttcgagtccg aagggtggga ggtctcgctc tccaccggag
     3721 gcgggcctcg gcgccgcctt ctcccgcggg tcgttcgagg cgatatccaa cgttgagcgc
     3781 agagtcacag gtccttcagc cgtccgatca agctcgatcc accgcgtctt ccggctcgga
     3841 aaggaagacg ccgactcggc ctcgtcgcgg ccgcatctgc gccctgccta gctgcacgac
     3901 ggtcctctcg acctacaacg cctcggacgt gtgctggttg cacacgccgc cgacgtttcg
     3961 gatcccccac gcctgaagcc cagttctccg taggactgac atgcgggctg accgccctct
     4021 gtcacgagcg acgcggtagg ctccccacgg ccgcctgaat cgatctcgac tcctcccgcc
     4081 tggctccgca gtgtgcgcac accgtggcac gtgtggagcc ggtctcatcc cctcggcaat
     4141 cggccctcac ctcgccgtac actcagtcac gagcgagcgt cgcatcatcc gaaaggagtt
     4201 ctcgaccacc ttcttctaca accctcgccc tcgtcgggcc ggtagaagac cgctacgacc
     4261 cacgtgcact gcaatatcgc aggggcccgg accgtgattt gccgatacct catgcagtac
     4321 tcggagatgc cgggtgcggg aggggggtct ggtggccgag caaacgttag acatgttccg
     4381 gtctgggttc gtgcccagga gcgggaagaa gcagaccgag atggccgcgt cgtacgagga
     4441 cggcgacgct cgactgcggt tgagttccgc ccaatgggtg gtggcgctga ggcccgtgat
     4501 tcgcggggat ccgatcgttg tcatcgcaac gcgatgtagg acatctccga accgggttcg
     4561 acgatggttg aagtcgccaa tctctcggag agcagtggcg gcggagatcg ctcagccgac
     4621 cgaggagcgc ttccgtctga gcatcaccct gcgtggcgtc caagaggcga actcattgcg
     4681 ccgtgagcgt gcggcgggga aggaactggt gaccggagat gagtaaggaa aaggggcagg
     4741 aaccagagca cactagggct cggagcgact tggaccagac ggatcgaaga ggcctcagcg
     4801 acaagcagca gctcgccatc gacctgctgc tcgtcggaac gaagtaccaa gatgtcgcga
     4861 aggcggtcta gcggtcgctc tcggcgc
//
LOCUS       DASUVE010000310         5183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1093806, whole genome shotgun sequence.
ACCESSION   DASUVE010000310 DASUVE010000000
VERSION     DASUVE010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093806"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VF036_06670"
     CDS             complement(<1..652)
                     /locus_tag="VF036_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6264948.1"
                     /translation="MELTDRIAAAGLEGERVRAEVVEGGETLEVDGLLVSLSNLPASE
                     LNGTRVIREPEDAPTALEAARAVFRGRGHPFFGIEVEVGRHPAVEEAIRAAGLKRVDG
                     WPTMAAPIGGLASEPVLPGIEIREAREHHDLEAVRAVEVTTFGTPRDIAERFVGPRML
                     EDDRVRMFTAWIDGEPVGEASAYLLHDTVGIFGVGVVEAARRRGIGAALTLRASRAF"
     gene            686..1390
                     /locus_tag="VF036_06675"
     CDS             686..1390
                     /locus_tag="VF036_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEX6264949.1"
                     /translation="MLPICAVIVDFDGTACLHDVGVDLLDRFGTDASRNRELAGIDEA
                     FDAGRIGLRDVLLAEAATLRADDDEMIAFALAHCPLDPTFSPFVAWAEHREVPITVVS
                     DGFGLHVRPLLAAAGLEHLSVVTNTWDRGTLAFGAAHPVCVGCGTCKKQAVERGRETH
                     GPVAFVGDGVSDRFGARYADVTFAKDGLAEHCRRASIPFLAYEDFDDVRRALLELDGS
                     LAPVGGEPCPGWREPS"
     gene            1372..2295
                     /locus_tag="VF036_06680"
     CDS             1372..2295
                     /locus_tag="VF036_06680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6264950.1"
                     /translation="MAGAVVTSLPAGLVARPLGETDLDAVVTMVNTCELHDTGEVMLE
                     RADLVADAGAEGFDPSADWIGVFDGDRIVGWGLLERRHRLATDVHPDARGRGVGAWLR
                     AWSEERARAAGSASVVQVIDDRREDVASMLMAAGYTPQGTSWILRIAHLDRPPRPVPP
                     RGIELRASRPEDEDDALLLFERAFSEWPGRQPTSPATWRAMVTEREGFEPEDLIIALA
                     DGVVVGGAFLIDADEIWVDKLAVASEFRHRGIARALLLTAFGRSFDRGHAWTALSTDS
                     RTGALSLYERIGMRVHRSFTRYGREPSARTS"
     gene            complement(2301..2388)
                     /locus_tag="VF036_06685"
     tRNA            complement(2301..2388)
                     /locus_tag="VF036_06685"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2352..2354),aa:Ser,seq:gga)
     gene            complement(2423..2962)
                     /locus_tag="VF036_06690"
     CDS             complement(2423..2962)
                     /locus_tag="VF036_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264951.1"
                     /translation="MTRIRSLLAVAAIGSIALTPSPAVGGIPPGHLAIGDSVMLGAKD
                     ELVARGFRVNAIVSRQFRDAVPLVEQLKAARRLRSKVIVHLGNNGILIQAADCNRISE
                     LAGRDRTVYLVTLKIPRWYRATENERLAACARRRANTMLVNWFSFSRHHPFWFAADGF
                     HLTPTGQARYAAFVATKTA"
     gene            complement(3007..3099)
                     /locus_tag="VF036_06695"
     tRNA            complement(3007..3099)
                     /locus_tag="VF036_06695"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3063..3065),aa:Ser,seq:cga)
     gene            3178..3567
                     /locus_tag="VF036_06700"
     CDS             3178..3567
                     /locus_tag="VF036_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6264952.1"
                     /translation="MDAKSLERVPLFAGLSPKEREKVARWADTVDLPPGKHLLDEGRL
                     PHEFFVIVDGEVEVLHDEQRLATLGPGDFFGEIALIEHGRRTASVVTTVPTTLAVMSP
                     ASFAAMRREMAEVALRIDDAIRERSER"
     gene            complement(3564..4028)
                     /locus_tag="VF036_06705"
     CDS             complement(3564..4028)
                     /locus_tag="VF036_06705"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX6264953.1"
                     /translation="MGLTAEPDDERVMRLALAEARTCLEHDDVPIGAAVTRGDDLLAA
                     VGNRRELDGDPTAHAEILALRAASAALGSWRLEGCTLTVTLEPCAMCAGAAVLARLDR
                     VVFGAADPKAGFARSLGNLLDDPRLNHSVASTGGVLADECGDLLRSFFRERR"
     gene            complement(4019..4369)
                     /locus_tag="VF036_06710"
     CDS             complement(4019..4369)
                     /locus_tag="VF036_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018566395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX6264954.1"
                     /translation="MSHRSRRRFESLVSDALDELPGWVQEAMDNVAVVVEDHPPADDP
                     DLLGLYEGIAQTEREFYVGALPDRITVFRYTIEAEAGEDDEELRAQVAETVVHEIAHH
                     FGISDERLEALGWD"
     gene            4389..>5183
                     /locus_tag="VF036_06715"
     CDS             4389..>5183
                     /locus_tag="VF036_06715"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019872347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX6264955.1"
                     /translation="MPGGLSTDLYELNMAASYLRRGMDGLATFSLYVRDMPKQRGFLV
                     SAGLEDCLRFLETYGFEDEELAYLREVGFDDRAIEDFRTTSFEGEVWAVPEGRIVHAK
                     EPIVEVTAPIAQAQLVEAVLLNQVTLHTTLTSKAARYMLAAEGRMLVDFAFRRAHGVE
                     AARAAARGSAIVGFEGTSNVEAAREFGLRVTGTMAHSFITAFDDERDAFRAFARLYPG
                     TTLLVDTYDTPAGVRHVIDLARELGAEFSVGAIRLDSGDLGELAAQA"
BASE COUNT          748 a         1767 c         1858 g          810 t
ORIGIN      
        1 cgaacgcgcg gctcgcgcgg agcgtgagcg cggcaccgat cccgcgccgc cgtgcggcct
       61 cgacgacacc gacgccgaag atccccaccg tgtcgtgcag gaggtacgcc gaagcctcgc
      121 cgaccggctc cccgtcgatc cacgcggtga acatccgcac ccgatcgtcc tcgagcatcc
      181 gggggccgac gaagcgctcc gcgatgtccc ggggcgttcc gaacgtggtg acctcgacgg
      241 ctcggaccgc ctcgaggtca tggtgctcgc gcgcctcgcg gatctcgatg cccggaagga
      301 cgggttccga tgcgagcccg ccgatcggtg cggccatcgt cggccacccg tccacccgct
      361 tgagcccggc cgcccggatc gcctcttcga ccgccggatg ccggccgacc tcgacctcga
      421 tcccgaagaa gggatgtcct cgcccccgga acacggcgcg agcggcctcc agcgccgtcg
      481 gcgcgtcctc cggctcccgg atcacccggg tcccgttcaa ctccgacgcc gggaggttcg
      541 agagggagac gaggaggccg tcgacctcca gggtctctcc tccttccacg acctcggcac
      601 gcacccgttc gccttccaag cccgcggccg cgatccggtc ggtgagctcc atcagggcat
      661 gctcccacgc cgttagcctc cgtggatgct gccgatctgc gccgtgatcg tcgacttcga
      721 cgggaccgcg tgtctccacg atgtgggggt cgatctgctc gatcgcttcg ggaccgacgc
      781 ctcgcggaac cgcgagctcg cggggatcga cgaggccttc gatgccggga ggatcggcct
      841 ccgagacgtg ctgctcgcgg aggccgcgac cctccgcgca gacgacgacg agatgatcgc
      901 gttcgcgctg gcgcactgcc cgctcgatcc gacgttctcg ccgttcgtcg catgggcgga
      961 gcaccgggag gtgccgatca cggtcgtgtc ggacgggttc gggctccacg tccgcccgtt
     1021 gctcgcggct gccgggctcg agcacctctc ggtggtcacc aacacctggg atcgaggcac
     1081 gctcgcgttc ggtgcagccc acccggtctg cgtcggctgc ggcacgtgca agaagcaggc
     1141 cgtcgagcgt gggcgtgaga cgcacggacc ggtggccttc gtcggcgacg gcgtgagcga
     1201 ccggttcggt gcccggtacg cggacgtcac gttcgcgaag gacggccttg ccgaacactg
     1261 ccgacgcgcg tcgatcccgt tcctcgccta cgaggacttc gacgacgtcc ggcgggcgct
     1321 cctcgagctc gacggctctc ttgcgccggt cggcggtgag ccgtgtcccg ggtggcggga
     1381 gccgtcgtga cctcgctgcc ggccggactc gtcgctcgcc cgctgggcga gaccgatctc
     1441 gacgccgtgg tcacgatggt gaacacgtgc gagctccacg acaccggcga ggtgatgctc
     1501 gagcgagccg atctcgtcgc ggatgccggc gccgaggggt tcgatccgtc cgcggattgg
     1561 atcggggtct tcgacggcga tcgcatcgtc ggctgggggc tcctcgagcg acgacatcgc
     1621 cttgccacgg acgtccaccc tgatgcccgg ggtcggggcg tgggtgcctg gctgcgggcg
     1681 tggtcggagg agcgcgcgcg ggcggccggg tcggcgagcg tcgtgcaggt gatcgacgac
     1741 cggcgtgaag acgtcgcatc gatgctgatg gctgcggggt acacgccgca ggggacgtcg
     1801 tggatcctcc ggatcgccca tctggatcgg cccccacgtc cggtgccgcc ccgaggcatc
     1861 gagctccgcg cctcccgtcc ggaggacgag gacgacgcgc tcttgctgtt cgagcgcgcg
     1921 ttctccgagt ggccgggtcg gcagcccaca tctccggcca cgtggcgggc gatggtcacg
     1981 gagcgggagg ggttcgagcc ggaggacctc atcatcgccc tggcggacgg ggtcgtggtc
     2041 ggaggtgcgt tcctgatcga tgcagacgag atctgggtcg acaagctcgc cgtggcgagc
     2101 gagttccgcc atcgagggat cgctcgtgcg ctgttgctga ccgcgttcgg gcgctccttc
     2161 gatcggggcc atgcgtggac ggcgttgtcg actgattcga ggaccggagc gctctccctg
     2221 tacgagcgga tcgggatgcg ggtccaccga tcgttcaccc ggtacggccg cgaaccgtcg
     2281 gctcgaacct cgtgacgaag cggtggccga gggattcgaa ccctcgaggg agtcatcccc
     2341 cctacacgct ttccaggcgt gtcccttcgg ccgctcgggc agaccaccgc gatgagttta
     2401 ccgacgcgat atcgaccgcc gatcaggcag tcttcgtcgc gacgaacgcc gcgtacctgg
     2461 cctggccggt cggcgtgagg tggaagccgt ccgccgcgaa ccagaacggg tggtgccggc
     2521 tgaaggagaa ccagttgacg agcatggtgt tcgcgcgccg ccgggcacac gcggccaacc
     2581 gctcgttctc ggtcgcgcgg taccaccgcg ggatcttcaa cgtcacgagg tacaccgtgc
     2641 ggtcccggcc tgcgagctcg gagatgcggt tgcagtcagc cgcctggatc aagatcccgt
     2701 tgttaccgag atggacgatc accttgctgc ggagccgtcg cgccgccttc agctgctcga
     2761 cgagcgggac cgcgtcgcgg aattgacgag agacgatggc attcacgcgg aacccccggg
     2821 ccaccagctc gtccttcgcc ccgagcatca ccgagtcacc gatcgcgaga tgccccggag
     2881 ggatgccccc gaccgcgggg gacggcgtga gcgcgatcga tccgatcgcg gccaccgcga
     2941 gcagggagcg gatgcgggtc atctcgtcag gtatcgatgg gcgggcccgt gagaggcccg
     3001 cccggatggc ggaggcggcg tgattcgaac acgcgagagg cggattaggc ccctaaccgc
     3061 tttcgaggcg gtcccgttcg tccactccgg cacgcctccg tccacgagtc tagcgggcga
     3121 gccgggggcg ggcgcgagcg tgacggcgga tggacgatcg tgcgaggatc cacgaacgtg
     3181 gatgcgaaga gcctggaacg tgtgcccctg ttcgcgggtc tctcccccaa ggagcgcgag
     3241 aaggtggcac gctgggccga caccgtggat ctgccgccgg gcaagcatct cctcgacgag
     3301 ggtcggctgc cgcacgagtt cttcgtgatc gtcgacggcg aggtcgaggt gctgcacgac
     3361 gagcagcgtc tcgcgacgtt gggccccggg gacttcttcg gagagatcgc gctgatcgag
     3421 cacggacgcc ggaccgcttc agtggtaacg accgtgccca cgaccctcgc cgtgatgagt
     3481 ccggcctcgt tcgcggcgat gcggcgtgag atggccgagg tcgctctcag gatcgacgac
     3541 gcgatccgtg aacggtcgga acgttagcgt cgctcgcgga agaacgaccg gagcagatcg
     3601 ccgcactcgt cggcgagcac gcctccggtg gacgcgaccg aatggttcaa cctcggatcg
     3661 tcgaggaggt tcccgagcga gcgggcgaat cccgccttcg ggtcggccgc tccgaacacc
     3721 acccgatcga gccgggcgag gacggcggcg ccggcacaca tcgcgcaggg ctcgagggtc
     3781 accgtgagcg tgcacccttc gagtcgccac gagccgaggg ccgcggatgc ggcgcgcaga
     3841 gccaggatct ccgcgtgcgc ggtcggatcg ccgtcgagct cgcgtcggtt ccccaccgcg
     3901 gcgagcaggt cgtcgcctcg ggtgaccgcg gcaccgatcg gcacatcgtc gtgctcgagg
     3961 caggtacgtg cttccgcgag cgcgagccgc atcacgcgct cgtcgtccgg ctccgcggtc
     4021 agtcccaccc cagggcctcg agccgctcgt ccgagatgcc gaagtggtgc gcgatctcgt
     4081 gcaccacggt ctcggcgacc tgggcgcgga gctcctcgtc gtcctcgccc gcctcggcct
     4141 cgatcgtgta gcggaacacc gtgatccgat cgggcagcgc tcccacgtag aactcgcgtt
     4201 ccgtctgcgc gatcccttcg tagaggccga ggaggtcggg atcgtcggcg ggcgggtggt
     4261 cctcgacgac cacggcgacg ttgtccatcg cctcctgcac ccagccgggc agctcgtcga
     4321 gggcgtccga cacgagcgac tcgaaccgac ggcgggagcg gtgcgacatc gcttgtagcc
     4381 tacgtcgcat gcccggcggg ctcagcacgg atctctacga gctcaacatg gcggcgagct
     4441 acctgcgtcg tgggatggac gggctcgcca cgttcagcct ctacgtgcgg gatatgccga
     4501 agcagcgtgg cttcctggtc tccgcgggcc tcgaagactg cctccgtttc ctcgagacgt
     4561 acgggttcga ggacgaggag ctcgcctacc tccgggaggt cggcttcgac gatcgagcga
     4621 tcgaggactt ccgaacgacc agtttcgagg gggaggtctg ggcggtgccc gaggggcgga
     4681 tcgtccatgc gaaggagccg atcgtcgagg tcaccgcgcc gatcgcgcag gcgcagctcg
     4741 tcgaggccgt gttgctgaat caggtgacgt tgcacacgac gctcacgtcg aaggccgctc
     4801 gctacatgct cgcggccgaa ggccggatgc tcgtagactt cgcgttccga cgcgcgcacg
     4861 gggtggaggc cgcccgcgca gccgctcgtg ggagcgcgat cgtgggcttc gagggcacgt
     4921 ccaacgtcga ggccgcgcgc gagttcgggc tgcgcgtgac ggggacgatg gcgcattcct
     4981 tcatcacggc gttcgacgac gagcgcgacg cgttccgcgc gttcgcgcgg ctgtatccgg
     5041 gcaccacgct gctcgtcgac acgtacgaca cgccggcagg cgtgcggcac gtcatcgatc
     5101 tggcgcgcga gctgggagcg gagttctcgg tgggcgcgat ccggctcgac tccggtgacc
     5161 tcggggagct cgcggcgcag gcg
//
LOCUS       DASUVE010000311         2333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1471173, whole genome shotgun sequence.
ACCESSION   DASUVE010000311 DASUVE010000000
VERSION     DASUVE010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471173"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1059)
                     /locus_tag="VF036_06720"
     CDS             complement(40..1059)
                     /locus_tag="VF036_06720"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6264956.1"
                     /translation="MTNGNGGVVIDIHAHVMTPEADEIAAPLFTPEKDPFIGFSGPAS
                     EAYNRSHFAEILPKLTQPEQRLRDMDRMGVDVQAISVVPPQYFYWPEPQIGEKLAGMV
                     NDRLYEIVESHPDRFVGLGTLPLQDVDLALGELDRVVRDLRFGGLEICTNVNGVDFDH
                     PRFVPFFEKVVEHDLLLVVHPNGFTQGQRLTDYYLINTLGMPMDSTVFIARMIFGGVL
                     ERFPTLKVCVTHGGGYLPFYSARFDHAYEARDDCREHISRPPTTYLAQMYFDTMVFDP
                     VMLSTLVRRWGADHVLLGTDYPFDMGEDDPLGLLDRVEGLGEPERWMISGGNAMRLLG
                     LDRSP"
     gene            complement(1147..>2333)
                     /locus_tag="VF036_06725"
     CDS             complement(1147..>2333)
                     /locus_tag="VF036_06725"
                     /inference="COORDINATES: protein motif:HMM:NF005566.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HEX6264957.1"
                     /translation="PPGRPRVAVVGGSLGGLITGLQLRDVGCEVDIFERSPVPLEGRG
                     AGIVLHPVTTRYFEEHGLLDLGQVSSSARTLRYLSATGDVLLEEPIVYRFTSYATLYA
                     ALVDFFDPSRYHLGKDLVDVSNRAAEVRARFADGSVRSFDLLVGADGIRSTVRGLLFP
                     DLSPTYAGYVGWRGTLSERELPTRAREELRHLLTYHVGDRTHVLSYEIPAPGGGSMNW
                     VWYRNVPDGSALASLLTDRSGVRHDLSLPAGGVHDDHLDELRGSAAQLPPAFTDLIRA
                     TPEPFLQVIVDLEVPAMVRGRICLVGDAAFALRPHIAAGTAKAAADARSLAEAIDAAG
                     DDVPAALAAWEPAQLALGRATTARTREVGERAQVTGTFRPGDPEVAFGLYRPKDGNYG
                     AH"
BASE COUNT          390 a          800 c          786 g          357 t
ORIGIN      
        1 tgatcgcggc ggtcggatcg gccgtcgccc tccccttcgt tatggcgagc gatccagacc
       61 caggagtcgc atagcgttcc cccccgagat catccagcgt tctggctctc cgagaccctc
      121 gacccggtcg agcaacccca agggatcgtc ctctcccatg tcgaacggat agtccgtacc
      181 caacaagacg tgatcggcac cccatcgccg cacgagtgtc gacagcatca cggggtcgaa
      241 caccatggtg tcgaaataca tctgcgcgag gtaggtggtc ggcggccggc tgatgtgctc
      301 gcgacaatcg tcgcgcgctt cgtacgcgtg atcgaagcgc gccgagtaga acggcaggta
      361 tccgccgccg tgcgtcacac agaccttcag cgtggggaac cgttccagaa ccccgccgaa
      421 gatcatccgc gcgatgaaca ccgtcgagtc catcggcatc ccgagcgtgt tgatcaggta
      481 gtagtccgtc aggcgctgcc cctgcgtgaa gccgttcgga tggacgacga ggaggagatc
      541 gtgctccacg accttctcga agaacggtac gaagcgcgga tggtcgaagt cgacgccgtt
      601 gacgttcgtg cagatctcca gcccgccgaa ccggaggtcg cgcacgaccc gatcgagctc
      661 accgagggcc aggtcgacgt cctggagagg aagcgtgccg aggccaacga agcgatccgg
      721 gtggctctcg acgatctcgt acaagcggtc gttcaccatc ccggcgagct tctccccgat
      781 ctgcggctcg ggccagtaga agtactgggg cggcaccacc gagatcgcct gcacgtcgac
      841 ccccatgcga tccatgtcac gcaaccgctg ctccggttgg gtgagcttgg gcaggatctc
      901 ggcgaagtgc gagcggttgt acgcttcgct ggcgggtccg gagaagccga tgaacgggtc
      961 cttctctgga gtgaacagcg gggctgcgat ctcgtcggct tcaggggtca tcacgtgcgc
     1021 gtggatgtcg atcaccacgc cgccgttgcc gttcgtcatc cccatcctcc gtcgttacgg
     1081 gcgtcgtccg ggggtcggga cgccgtcgca ggtctgacat tatatacaac ttgcgtggag
     1141 gcccgtctag tgcgcgccgt agttcccgtc cttcggtcgg tagaggccga acgccacctc
     1201 cgggtcgccg ggacggaagg tgccggtgac ctgcgcgcgc tcccccacct ccctcgtgcg
     1261 agccgtggtg gcgcgaccca gcgcgagctg cgcgggctcc cacgccgcga gcgccgccgg
     1321 cacgtcgtca ccggccgcgt cgatcgcctc cgcgagggag cgagcatccg cggccgcctt
     1381 cgccgtgccg gcggcgatgt gcggccggag cgcgaacgcg gcatcgccga cgagacagat
     1441 cctcccgcgc accatcgcgg gcacctcgag atccacgatc acttggagga acggctccgg
     1501 ggtcgcgcgg atcagatccg tgaacgccgg cggcagctgc gccgccgacc cgcggagctc
     1561 gtcgaggtga tcgtcgtgca caccgcccgc cggcagcgat agatcgtgac gcacgccgga
     1621 ccggtccgtg agcagcgacg ccagcgcact cccgtccggg acgttccggt accacaccca
     1681 gttcatcgaa ccaccgccgg gcgcggggat ctcgtaggac agcacgtgcg tccgatcgcc
     1741 gacgtggtag gtgaggaggt gcctgagctc ctcgcgggcc cgggtcggca gctcgcgttc
     1801 ggagagcgtc cctcgccacc cgacgtagcc ggcgtaggtg ggagacaggt cggggaacag
     1861 cagtccgcgc acggtcgatc ggatgccgtc cgctcccacg agcaggtcga aggaccggac
     1921 gctcccgtcg gcgaagcggg ctcggacctc ggcggctcgg ttcgagacgt ccacgagatc
     1981 cttccccagg tgatagcgcg acgggtcgaa gaagtcgacg agcgctgcat agagggtcgc
     2041 gtaggacgtg aaccgataca cgatcggttc ctcgaggagc acgtctcccg tcgccgagag
     2101 gtaccggagc gtccgcgccg acgagctcac ctggccgagg tcgagcagtc cgtgctcctc
     2161 gaagtagcgg gtcgtgacgg ggtggagcac gatccccgcc ccccgaccct ccagcggcac
     2221 gggcgaccgt tcgaagatgt ccacctcgca cccgacgtcg cgcagctgca gcccggtgat
     2281 cagtccgccg agggagcccc cgacgacggc gacgcgcggc ctccccgggg gca
//
LOCUS       DASUVE010000312         7007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1471224, whole genome shotgun sequence.
ACCESSION   DASUVE010000312 DASUVE010000000
VERSION     DASUVE010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471224"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VF036_06730"
     CDS             complement(<1..499)
                     /locus_tag="VF036_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264958.1"
                     /translation="MTRFLGSARAASVTLTVVLSITLFAASSALAGHLSDWTHDHSDA
                     GVPPRPSGLAGLNDVFGPRCSDKANNARSWWPHADTDPFGAGKYVFYHTYLARNIGFN
                     VRNHINAAHRDGALYPGIGGYNCRQIAGSTSWSVHSWGAAIDTNWQRNPRFQTHWNGK
                     GIDGTN"
     gene            complement(635..2587)
                     /locus_tag="VF036_06735"
     CDS             complement(635..2587)
                     /locus_tag="VF036_06735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX6264959.1"
                     /translation="MSPNARADTPTPELLLDVRTPAELAFSPDETRLAFALHATVADE
                     GSFVPSDLYVIDTDGHHPPVQLTSEPWSDRTPAWSPDGSQLAFLSDRVTPGHQLPYTM
                     RATRTPAAPRLAATLTGSAESVAWSSDGTRLLVLAADPGTYGLDWSARAVNGATPRPD
                     PSPRRPGDARRRLFLVDLATNRAEEVGPEDRSVWEFDWDGDDIVVALVSEDHSGSGWY
                     QSVVARLSLDTRTAETIYEPTWQTGSLALSPDATRAIVVEGYASDHGLLAGTIVVIDL
                     ATSTTADPSPDLQTVGLASWCDDDSLWYGRTDGTGTACGRLHLDGRHSEPWRDDAFIG
                     DAVTTPACAVSRDGTHVWTTHQAHALPPELARFDHATASWERLTTFNDRIVEGRIFPD
                     SRTISWKGEDGTEIEGILMTPQGTSGPLPTIVCVHGGPTWNWGAYFSDSEPSAVLLAS
                     TGYACLLPNPRGSIGRGHAFAQGVIGDGGGIDYRDIMAGVDHCIAEGITDPDRLGIAG
                     LSYGGYMAGWAVGQTDRFRAAVAMSVVSNYVSFHLTSEVWWYDQAILRGEWNDPASQY
                     MERSPVTHARRCTTPTLIIQGAEDRCTPVGQAEELFHAIASSGTEVELVVYPREGHVP
                     MERAHALDAIVRTQAWFDEHLISERG"
     gene            complement(2660..3184)
                     /locus_tag="VF036_06740"
     CDS             complement(2660..3184)
                     /locus_tag="VF036_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264960.1"
                     /translation="MPLFEAAILAQFVIHARGRQAVRNSLANEIAELREEQLAGDVKK
                     LARTKWLHANGAEASFPCGVVHIEDEAVPVLVAIVPDGIAFLAETPPGASNYDVLEVG
                     RIPRSDLAEVDVVDAAGQRVPEPLRESFEPDVDVSVVLRWTRGGEEQEDRFAFRSQWT
                     AWQAARRLREARKG"
     gene            complement(3187..5871)
                     /gene="ppdK"
                     /locus_tag="VF036_06745"
     CDS             complement(3187..5871)
                     /gene="ppdK"
                     /locus_tag="VF036_06745"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX6264961.1"
                     /translation="MTKYVYDFSEGNKDMKDLLGGKGANLAEMTTMGLPVPPGFTITT
                     EACLVFLAEGGAPAEMLQEAGEHLDTLQDAMGRQLGDPDDPLLVSVRSGAKFSMPGMM
                     DTVLNLGLNDRSVEGLARQSEGDVRFARDAYRRFIQMFGKIVMDVPGEAFEEALDRAK
                     ERKGAGTQDTDLDADELTALIEEFRGIYRTHRGEEFPQDPKEQLRLAIAAVFQSWNGK
                     RAIDYRRQNKISDDLGTAVNIQAMVFGNRGDDSGTGVAFTRDPSNGEKVPYGDYLANA
                     QGEDVVAGIRNTLPLVQLEELDPDSYRGLREHMDTLEHHYRDMCDIEFTIEKRKLWIL
                     QVRVGKRTAFAEWVMAHDMLGESLIDADTALLRVDANRLEELFKRRVRADGATPIAKG
                     LNASPGAAIGSVVFTADEAERLGKQGERVILVRRETTPDDYHGMIAAQGILTSAGGTN
                     SHAAVVARGEGIPAVCGADAIKLSPADRTFSANGTSVKEGDVITIDGFTGNVFVGELP
                     LEESVLEKARAGEAQAREEKIWKAFEALMAHADEVRRLRVRANADTPDQAKNARERGA
                     EGIGLCRTEHMFLGEERVGAVRQMIFADTEEEEAKAYDALAPLQREDFVGIFRAMDGL
                     PVTVRLLDPPLHEFLPNQVDLAVEVALAESRGEDVVERKAVLQKVNDLHEANPMLGLR
                     GVRLGIVKPGLYAMQVRAIFEAAGQLRREGGDPQVEVMIPLVATAPELRQMRDELEPV
                     AAEALGETALSHLEWGTMVELPRAAVTAGEIAETAEFFSFGTNDLTQTAFGFSRDDIG
                     KFVGMYEERKLVPANPFVTIDVDGVGRLMRIASEEGRASNPELHVGICGEHGGDPSSV
                     MFCHEIGLDYVSCSPFRVETARLAAGQAAVGAAESASK"
     gene            5942..6085
                     /locus_tag="VF036_06750"
     CDS             5942..6085
                     /locus_tag="VF036_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264962.1"
                     /translation="MAHAKDKGAKNVKKKAAKGIKEKRAEKRLKHDGRRQSGAVETIG
                     ERN"
     gene            complement(6191..6589)
                     /locus_tag="VF036_06755"
     CDS             complement(6191..6589)
                     /locus_tag="VF036_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264963.1"
                     /translation="MAEPVIVLDTSEIREGKREELERGIAELADFVEANETDPISYEV
                     FFGDDGRVMTVLQVHPDSASMERHMELAGPVFAKFADLVVLRAIDIYGSPSEKVVEQL
                     RRKAELLGTATVTVHDRRAGFARFGSRIAG"
     gene            complement(6653..>7007)
                     /locus_tag="VF036_06760"
     CDS             complement(6653..>7007)
                     /locus_tag="VF036_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264964.1"
                     /translation="PTFGEGWRDLDVMVVGELWLKILLHLRLDDVVAAPAAAGWNGGI
                     YRAWTDGDDVAVIMSTVWDTPKDAQEFSEALQRWVSRGTLRGLVTDADGTTVHAGFGS
                     REPLMAAVLSILRSL"
BASE COUNT         1038 a         2504 c         2345 g         1120 t
ORIGIN      
        1 agttggtgcc gtcgatgccc ttcccgttcc agtgcgtctg gaaccgcggg ttccgctgcc
       61 agttcgtgtc gatcgcggct ccccacgaat gcaccgacca agacgtcgac cccgcgatct
      121 gccggcagtt gtagccgccg atccccggat acagcgcccc gtcgcggtgc gcggcgttga
      181 tgtggttgcg gacgttgaag ccgatgttcc gcgccaggta cgtgtggtag aagacgtact
      241 tcccggcccc gaacggatcc gtgtccgcgt gcggccacca cgaccgggca ttgttggcct
      301 tgtcgctaca tcgcggaccg aacacgtcgt tgaggcctgc gagaccgctc ggccgcggcg
      361 ggactcccgc atccgagtgg tcgtgcgtcc aatcggagag atggcccgcg agcgcagacg
      421 acgcagcgaa caacgtgatc gagaggacca cggtcagcgt gaccgatgcg gctcgagcgc
      481 tgccgagaaa gcgtgtcatg acatcccctc cttctccttc gcgggggagg agggaggacg
      541 cgcgacgcta tcccatgggc gagggcggca ccgtgacgta cctcacgacg acgtggatgc
      601 cgatgctccg tgcgaaggct cgtccctgtc gcggctatcc gcgctcggag atgaggtgct
      661 cgtcgaacca ggcctgtgtc cggacgatcg cgtcgagcgc gtgggcccgc tccatcggca
      721 cgtgcccctc gcgcgggtag acgacgagct ccacctcggt tccggaggac gcgatcgcgt
      781 ggaacagctc ctcggcctgc ccgacgggtg tacagcgatc ctccgccccc tggatgatca
      841 gcgtcggtgt ggtgcaccgg cgagcatgcg tgaccggtga tcgctccata tattgactcg
      901 ccgggtcgtt ccactcccct cgcaggatcg cctggtcgta ccaccacacc tccgatgtca
      961 gatggaacga gacgtagttc gagaccacgg acatcgcgac ggccgcgcgg aaccgatccg
     1021 tctgcccgac ggcccagccc gccatgtacc cgccgtagga caggcccgcg atcccgaggc
     1081 ggtccgggtc cgtgatgccc tcggcgatgc agtggtcgac gccggccatg atgtcccggt
     1141 agtcgatccc gcccccgtct ccgatcacgc cttgagcgaa cgcgtggccg cgcccgatgc
     1201 tccctcgcgg gttcgggagc aagcacgcgt agccggtgga cgcgagcagc accgcgctcg
     1261 gctcggagtc ggagaagtag gcgccccagt tccacgtggg cccgccgtgc acgcacacga
     1321 tcgtggggag cggcccgctg gtgccctggg gcgtcatgag gatcccctcg atctccgtgc
     1381 cgtcctcacc tttccaggag atcgttcgcg agtccgggaa gatgcgaccc tcgacgatgc
     1441 ggtcgttgaa ggtcgtgagc cgctcccacg aggcggtcgc gtgatcgaac cgggcgagct
     1501 caggcgggag ggcgtgcgcc tggtgggttg tccagacgtg ggtgccgtcg cgcgagacgg
     1561 cgcaggcggg cgtcgtcacg gcgtcgccga tgaaggcatc gtcgcgccac ggttccgagt
     1621 gtcggccgtc gaggtggagc cggccgcagg cggtcccggt cccgtccgtg cgtccgtacc
     1681 agagggagtc gtcgtcgcac cacgatgcga gtccgaccgt ctgcaggtcg ggcgaggggt
     1741 cggccgtggt gctggtggcg agatcgatca cgacgatggt gccggcgagc agaccgtggt
     1801 cgctcgcgta gccctcgacg acgatcgccc gggtggcgtc gggcgagagc gccaggcttc
     1861 ccgtctgcca tgtgggttcg tagatcgtct ccgccgtccg ggtgtcgagg gacaggcggg
     1921 ccacgaccga ctggtaccag ccggagccgg agtggtcctc cgagacgagc gcgacgacga
     1981 tgtcgtcgcc gtcccagtcg aattcccaca cgctgcgatc ctccgggccg acctcctcgg
     2041 cgcggttggt cgcgagatcg acgaggaaca ggcgacggcg cgcgtcgccc gggcgtctcg
     2101 ggctcgggtc gggacgtgga gtggcgccgt tgaccgcgcg ggcgctccaa tccaggccgt
     2161 aagtgccggg atcggccgcg aggacgagga ggcgggtgcc gtcgctcgac caggcgacgc
     2221 tctcggcgga gcccgtgagg gtggcggcga gacggggcgc tgcgggggtc cgggtggccc
     2281 gcatcgtgta cgggagctga tggcccggcg tgacccgatc ggagaggaac gcgagctgcg
     2341 atccgtccgg cgaccatgcg ggcgtccggt cgctccacgg ctcggaggtg agctggacgg
     2401 gcgggtggtg cccatcggtg tcgatcacgt agaggtcgct cgggacgaac gacccctcgt
     2461 cggccacggt ggcgtggagc gcgaaggcga gccgggtctc gtcgggcgag aaggcgagct
     2521 ccgctggcgt acggacgtcg aggaggagct cgggggtggg tgtgtctgcg cgtgcgttcg
     2581 gcgacatggc gcgtatcttc ccggagccgg cgggttccgt cgcgttccgg tcccggaagc
     2641 tcgtcggatg tgctccgagc tagcccttcc gcgcctcccg gagacgccgc gcagcttgcc
     2701 acgccgtcca ctgcgagcgg aacgcgaacc gatcctcctg ctcctcgccg ccccgcgtcc
     2761 accggagcac gaccgagacg tcgacgtcgg gctcgaacga ctcgcggagc ggttccggca
     2821 cacgctgccc cgccgcatcc acgacgtcga cctcggcgag atctgatcgc gggatccgac
     2881 ccacctccag gacgtcgtag ttggacgccc ccggcggtgt ctcggcgagg aacgcgatcc
     2941 cgtcgggcac gatcgccacg agcacgggga cagcttcgtc ctcgatgtgg acgactccgc
     3001 acgggaagga ggcctccgcg ccgttcgcat gcagccactt cgtccgcgcc agcttcttca
     3061 cgtcgccggc gagctgctcc tcacggagct ccgcgatctc gttcgcgagg ctgttccgga
     3121 ccgcctgtcg gccgcgggca tggatcacga actgcgccag gatcgccgcc tcgaagagcg
     3181 gcatccctac ttcgaggcgg actcggccgc gcccacggcc gcctggccgg cggcgaggcg
     3241 tgccgtctcc acccggaacg gcgagcagct cacgtagtcg agtccaatct cgtggcagaa
     3301 catcacgctc gatgggtcgc ccccgtgctc gccgcagatc ccgacatgga gctcggggtt
     3361 cgaggcgcgc ccctcctcgc tcgcgatccg catcaacctg ccgaccccgt cgacgtcgat
     3421 cgtgacgaac gggttcgcgg gcacgagctt gcgctcctcg tacatgccca cgaacttgcc
     3481 gatgtcgtcg cgcgagaacc cgaacgcggt ctgcgtcagg tcgttggtcc cgaacgagaa
     3541 gaactctgcc gtctccgcga tctccccggc ggtcactgct gcgcgcggca gctcgaccat
     3601 cgtgccccac tcgaggtgcg agagcgccgt ctcgcccagc gcttccgccg cgaccggttc
     3661 cagctcgtcg cgcatctgtc tgagctccgg cgccgtcgcg accagcggga tcatgacctc
     3721 gacctgcgga tcgccaccct cgcgtcgcag ctgacccgcg gcctcgaaga tcgcacgcac
     3781 ctgcatcgcg tagaggcccg gcttcacgat cccgagccgc acgccccgca gacccagcat
     3841 cggattcgcc tcgtgcaggt cattcacctt ctgcagcacg gccttccgct ccacgacgtc
     3901 ctcgccgcgg gactccgcga gcgccacctc caccgccagg tccacctggt tcggcaggaa
     3961 ctcatgcaac ggcgggtcga gcagccgcac cgtcacgggc agaccatcca tcgcccggaa
     4021 gatcccgacg aagtcctcgc gctgcagggg cgccagcgcg tcgtaggcct tcgcctcctc
     4081 ttcctcggtg tcggcgaaga tcatctgccg caccgctccc acgcgctcct cgccgaggaa
     4141 catgtgctcc gtgcggcaca ggccgatccc ctctgcgccg cgctcgcgcg cgttcttcgc
     4201 ctggtcgggc gtgtcggcgt tggcgcgcac ccgcagccgc cgcacctcgt cggcgtgggc
     4261 catcagcgcc tcgaacgcct tccagatctt ctcctcgcgc gcctgcgcct ctccggcgcg
     4321 ggccttctcg agcacggact cctcgagcgg cagctccccc acgaacacgt tgcccgtgaa
     4381 gccgtcgatc gtgatgacgt caccctcctt cacggaggtg ccgttcgcgg agaacgtccg
     4441 gtcggccggc gagagcttga tcgcgtcggc gccgcacacc gccgggatgc cctcgccacg
     4501 ggcgaccacg gccgcgtggg agttcgtgcc tccggcgctc gtgaggatgc cctgcgccgc
     4561 gatcatcccg tggtagtcgt cgggcgtcgt ctcgcgccgg accaggatca cgcgctctcc
     4621 ctgcttcccg agccgctcgg cctcgtccgc cgtgaacacc accgacccga tcgcggcacc
     4681 gggcgaggcg ttcagaccct tcgcgatcgg cgtggcgccg tcggccctca cgcggcgctt
     4741 gaacagctcc tcgagccggt tcgcgtccac gcgcaggagc gcggtgtcgg catcgatcag
     4801 ggactccccc agcatgtcgt gcgccatcac ccactcggcg aacgccgttc gcttgcccac
     4861 gcggacctgg aggatccaga gcttccgctt ctcgatcgtg aactcgatgt cgcacatgtc
     4921 gcgatagtgg tgctcaagcg tgtccatgtg ctcgcggagg cctcgatacg agtcagggtc
     4981 gagctcctcg agctggacga gcggcagcgt gttgcggatc cccgcgacca cgtcctcgcc
     5041 ctgcgcgttc gcgaggtaat cgccgtacgg gaccttctcc ccgttggacg ggtcgcgcgt
     5101 gaacgccacc cccgtccccg agtcgtcgcc gcggttgccg aacaccatcg cctggatgtt
     5161 gaccgccgtg ccgaggtcgt cggagatctt gttctgccgc cggtagtcga tggcccgttt
     5221 gccgttccac gactggaaca ccgccgcgat cgccagacgc agctgctcct tcggatcctg
     5281 cgggaactcc tcccccctgt gcgtgcggta gatgccgcgg aactcctcga tcagcgcggt
     5341 cagctcgtcg gcgtcgaggt cggtgtcctg ggtgcccgcg cccttgcgct ccttcgctcg
     5401 gtcgagcgcc tcctcgaacg cctcccccgg cacgtccatc acgatcttgc cgaacatctg
     5461 gatgaatcgg cggtaggcgt cgcgggcgaa gcggacgtcg ccctccgact gccgcgcgag
     5521 gccctcgacg gacctgtcgt tcaggccgag gttcaggacc gtgtccatca tccccggcat
     5581 cgagaacttg gccccgctgc gcacgctcac gaggagcggg tcgtcggggt cgccgagctg
     5641 acgacccatc gcatcctgaa gggtgtccag gtgctctccg gcctcctgca gcatctccgc
     5701 cggggccccg ccctccgcga ggaacaccag gcaggcctcg gtggtgatcg tgaagcccgg
     5761 ggggaccggc agccccatcg tggtcatctc ggccaggttg gcgcccttcc cgccgaggag
     5821 gtccttcatg tccttgttgc cctcggagaa gtcgtacacg tacttggtca tgctccgagt
     5881 gtacggaagg ggcgcctgcc ggtgtacgct cgcctcctca cgcgcgacgg aggttcgacg
     5941 gatggcacac gccaaggaca agggcgcgaa gaacgtcaag aagaaggccg cgaagggcat
     6001 caaggagaag cgggccgaga agaggctgaa gcacgacggc cgccgccagt cgggcgccgt
     6061 cgagaccatc ggcgaacgga actaggggcc cctcgatcct gatcgagtct cgaacccggc
     6121 tcgatccggc acgcacccgt tccatccctc ggaggatgga tgcgcttacc agaggctgga
     6181 cgttcgtcgc tcagccggcg atgcgggagc cgaagcgcgc gaagccggcc cggcgatcgt
     6241 gcacggtgac ggtcgcggtg ccgagaagct cggccttccg ccggagctgc tccaccacct
     6301 tctcgctcgg tgagccgtag atgtcgatgg cccgcagcac caccaggtcc gcgaacttcg
     6361 cgaacaccgg cccggcgagc tccatgtgac gctccatcga cgccgagtcg ggatggacct
     6421 ggaggaccgt catgacgcga ccgtcgtcgc cgaagaagac ctcatacgag atcggatccg
     6481 tttcgttcgc ctccacgaag tcggccagct ccgcgatgcc tcgctcgagc tcctcgcgct
     6541 tcccctcgcg gatctccgat gtgtcgagaa cgatgacagg ctccgccata gctcggaacc
     6601 tagaacagac acttcgcgca agcaacgcga gcaccccgac cccgtgcgtc tgctacagcg
     6661 accgcaggat cgacagcacc gcggccatca ggggctcgcg ggagccgaag cccgcgtgca
     6721 ccgtcgtgcc gtcagcgtcc gtcaccaggc cgcgcagcgt gcctcgtgac acccagcgtt
     6781 ggagcgcttc gctgaactcc tgcgcgtcct tgggtgtgtc ccagacggtg ctcatgatca
     6841 ccgccacgtc gtcgccatcg gtccaggcgc ggtagatccc gccgttccat ccagccgccg
     6901 ccggagccgc cacgacgtca tcgaggcgga gatgcaacaa gatcttcagc cacagctcgc
     6961 ccacaaccat cacgtccagg tcgcgccagc cctcaccgaa cgtgggc
//
LOCUS       DASUVE010000313         3959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_719437, whole genome shotgun sequence.
ACCESSION   DASUVE010000313 DASUVE010000000
VERSION     DASUVE010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719437"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..670
                     /locus_tag="VF036_06765"
     CDS             185..670
                     /locus_tag="VF036_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX6264965.1"
                     /translation="MRRTRTALTRIALVSALMTVLTALAPAPAGAGTLSPRARMYRAT
                     NTSRVLNDVRRVDMHWQISKLARKHSIKMANTPDGSGGFGKLFHTKDPASYYLKSTSW
                     STWGENVGVTGGSVADLQQAFMDSPVHRANIVNHRFRRVALGTYRDDDGLLWVTVFFY
                     G"
     gene            complement(689..1177)
                     /locus_tag="VF036_06770"
     CDS             complement(689..1177)
                     /locus_tag="VF036_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX6264966.1"
                     /translation="MIEMQLEGVRVELPTNQPIVLLRERDGERYLPIWIGASEAAAIA
                     LSLQGVQTPRPMTHDLMKNILEDLSVAVQKIVVTELRDSTFYATIELQRNGDHYEVSS
                     RPSDAIALAVRLAVPIFASEDVLDEASILIPGEEDEEVEKFREFLDQVTPEDFKPEGP
                     PE"
     gene            complement(1222..1800)
                     /gene="hpt"
                     /locus_tag="VF036_06775"
     CDS             complement(1222..1800)
                     /gene="hpt"
                     /locus_tag="VF036_06775"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX6264967.1"
                     /translation="MTELARVLYGREDIHRRVDELGRTITGDYVGREPVLISVLKGGT
                     VFLADLMRQIPLPLETHLMAISPYGGHEVSLGRVRILLDVEIDLTDRDVLLVEDIVDT
                     GLTLSYLLSILRSRNPASLEVCTLLDRSIRRIVPLTPRYVGFDCPDRFVVGYGLDFEQ
                     RYRNLSDILEISDTAALKADPDALLPHLAVPA"
     gene            complement(1875..3146)
                     /locus_tag="VF036_06780"
     CDS             complement(1875..3146)
                     /locus_tag="VF036_06780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX6264968.1"
                     /translation="MKRRAAAGAAAFLPLLLLIAQASWLQAIGAPAAPPSTPVPPNGS
                     PSPFVSRLSTPADPVPVPRIDAPAVVLMDLAAGQVLYQRGAATARPVASLTKLMTALL
                     VLEAEEGRLDRTIRVDPDAVFGRGQYGAGSSLGLRPGERVSVRGLLAGLLLGSANDAA
                     EALAIGESGSVSAFVTAMNARARALGMAETTFASPHGLDDRGLSSASDVLTLLREVRR
                     HAAFRRLVASRLVVVRSDSAPPRRIQNRNVMLWLYPGASGVKTGSTAGAGYCLVATAE
                     RGGRELTAVVLGGRDEVFSEAAALLNHGFAAYHMRTLVSEGAPLGSARVRGGTVPVVA
                     GEELEALVRVQDTGGVDRIVTAARSVAYPPASGSEVGTLRVTSGGVVLGQVPVLVADL
                     PPPEEPRGSWWGRAVGAVSSALGGVIADLMD"
     gene            complement(3143..3868)
                     /locus_tag="VF036_06785"
     CDS             complement(3143..3868)
                     /locus_tag="VF036_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6264969.1"
                     /translation="MAGTRNYQSIGEVLVSVKTEFPDITISKIRFLEAEGLIEPERTP
                     SGYRKFYRHDVDRLKSILRLQRDEYLPLKVIKERLLHQDDGVDDVGAASDGEATDGDD
                     VSATDEIADAPTGLQMSLEEMSTATGIDRDRIKELEAFGIISTHGPEGARYYDGDDFI
                     ILSIVKDFFRYGIEARHLTMYKHFADRESSFFDQIVAPTLRQKNPDARRTAAQTLANL
                     SSTSRKFKQALLRTNLRDNLRSA"
BASE COUNT          667 a         1361 c         1321 g          610 t
ORIGIN      
        1 acagctcggt aattacgacg gtgtcgttgc agcgtatgcc cataggaaat ccgtgtcaat
       61 ggaaggcgag caaggggcgg gatccccccg cccggccctc atcggctcaa gttctgagcg
      121 ccttccgtcg atggaatagc tgcactaccc ggaacgaggg accggccgac gagggacaca
      181 ccggatgaga cgaacccgaa ccgcactcac gaggatcgcc ctcgtatcgg ctctcatgac
      241 ggtcctgacc gcgctcgctc ccgctccggc gggcgccggg acactctcgc cgagagcgcg
      301 gatgtaccga gccaccaaca ccagccgcgt gctcaacgac gtccgacggg tggacatgca
      361 ctggcagatc tcgaagctcg cgcgcaagca cagcatcaag atggcgaata ctccggatgg
      421 ttccggaggg ttcggcaagc tcttccacac gaaggaccct gccagctact acctcaagag
      481 caccagctgg tcgacctggg gagagaacgt cggcgtcacc ggtggatcgg tcgcggatct
      541 gcagcaggcg ttcatggact cccccgtcca ccgcgcgaac atcgtgaatc accgcttccg
      601 acgcgtcgcg ctcggcacct accgggacga cgacggcctg ctctgggtga ccgtcttctt
      661 ctacggctga tctcgtcggc ctaccgactc attccggcgg gccttcgggc ttgaagtcct
      721 cgggcgtcac ttggtcgagg aactccctga acttctcgac ctcttcgtcc tcctcgccgg
      781 ggatcaggat cgacgcctcg tcgagcacgt cctcgctggc gaagatcggg acggcgagcc
      841 gcacggccaa ggcgatcgca tccgagggtc gggagctcac ctcgtagtgg tccccgttcc
      901 gctggagctc gatcgtcgcg tagaacgtcg agtctcggag ctccgtcacg acgatcttct
      961 gcacggcgac ggagagatcc tcgaggatgt tcttcatcag gtcatgggtc atcggcctgg
     1021 gggtctggac cccttgcagt gagagcgcga tcgcggccgc ctcggaggcc ccgatccaga
     1081 tcggcaggta ccgctccccg tctcgctccc gcagcaacac gatgggctgg ttggtcggaa
     1141 gctccacgcg aacgccttcc agctgcatct cgatcatgtg ttggcactgt acgaggggcc
     1201 tgcgaccgag cgcaacctcc ctcaggcggg gacggcgaga tgcgggagca gggcgtccgg
     1261 atcggccttg agcgccgcgg tatcggagat ctccaggatg tcggaaaggt tccggtaccg
     1321 ctgctcgaag tcgaggccgt acccgacgac gaagcggtcc gggcaatcga accccacgta
     1381 tcggggggtg agagggacga tccgccggat cgatcggtcg aggagcgtgc acacctcgag
     1441 cgaagcgggg ttgcgggacc gcaggatcga caggagatag gagagcgtga gccccgtgtc
     1501 gacgatgtct tcgacgagca agacatcgcg gtccgtcaga tcgatctcga cgtcgaggag
     1561 gatgcgtacc cgtcccagcg acacctcgtg accgccgtag gggctgattg ccatcagatg
     1621 ggtctccagc ggcaacggga tctggcgcat cagatccgcg aggaacaccg tgccgccctt
     1681 gagcaccgag atcaggacgg gctcacgccc cacgtagtcc ccggtgatcg tgcggccgag
     1741 ctcgtccacg cggcggtgga tgtcctcgcg cccgtacagc acgcgagcga gctccgtcat
     1801 cgggttcgga gcctagcatc cgttcggcag gagctggtgg agcccggcca aggtcggcgg
     1861 cgaacccgcg tcgatcagtc catgagatcg gcgatcacgc cgcccagcgc ggacgagacg
     1921 gcgccgacgg cccggcccca ccaggagccg cggggctcct ccgggggagg gaggtccgcc
     1981 accaggacgg gcacctgccc gagaacaact ccgcccgagg tcacgcggag ggtccccact
     2041 tcggagccgc tcgcgggagg gtacgcgacc gacctcgctg ccgtgacgat ccgatcgacc
     2101 cccccggtgt cctgcacgcg cacgagcgcc tccagctcct cccccgccac gacgggcacg
     2161 gtgccgccac gcacccgagc gctcccgagc ggcgctcctt ccgagaccaa ggtccgcatg
     2221 tgatacgccg cgaacccgtg gttcaggagc gcggccgcct cggagaacac ctcgtcgcga
     2281 ccgccgagga cgacggcagt cagctctcgc ccgccgcgtt cggcggtcgc gacgaggcag
     2341 taccccgcgc ccgccgtgga cccagtcttc accccggaag ctcccggata cagccacagc
     2401 atcacgttgc gattctggat ccgccgtggc ggcgccgagt ccgagcggac gacgacgagc
     2461 ctgctggcca cgagccgccg gaacgccgcg tgcctccgaa cttcgcgtag cagcgtgagg
     2521 acgtcggatg ccgacgacag acctcggtcg tccaacccgt ggggagacgc gaaggtcgtc
     2581 tccgccatcc cgagggcgcg cgctcgcgcg ttcatcgccg tgacgaacgc gctgacgctc
     2641 cccgactccc cgatcgcgag cgcctccgcg gcgtcgttcg cggacccgag gagcaaaccg
     2701 gcaagcaacc cccggaccga gacccgctcg ccaggccgga ggccgagcga tgaccccgcc
     2761 ccgtactgcc cccggccgaa gacggcgtcg ggatcgacac ggatggttcg atccagacgc
     2821 ccctcctcgg cttcgaggac gagcaacgcc gtcatcagct tcgtcaggct cgcgaccggt
     2881 cgcgccgtcg cagcgcctcg ctgatacagg acctgcccgg cggcgagatc catcaagacg
     2941 acggccgggg catcgatccg tgggaccggg accgggtcgg ccggcgtgga cagccgcgac
     3001 acgaacgggg acggcgatcc gttgggtggc accggcgtcg agggtggcgc ggccggggcc
     3061 ccgatcgcct gcagccacga cgcctgggcg atcaggagca ggagtggcag gaaggcggcc
     3121 gcgccggcgg cggccctccg cttcacgcgc tgcgaaggtt gtcgcgcagg ttcgtccgca
     3181 acagggcttg cttgaacttc cgcgacgtgg acgacaggtt cgcgagggtc tgggccgcgg
     3241 tgcggcgggc gtccgggttc ttctgccgga gggtcggcgc cacgatctgg tcgaagaagg
     3301 acgactcccg gtccgcgaag tgcttgtaca tcgtgagatg acgagcctcg atgccgtagc
     3361 ggaagaagtc cttcacgatc gacaggatga tgaagtcgtc cccgtcgtaa taccgggcgc
     3421 cctcggggcc gtgcgtggag atgatgccga acgcttcgag ctccttgatc cggtcgcgat
     3481 cgatccccgt ggcggtggac atctcctcga gcgacatctg cagcccggtt ggtgcatccg
     3541 cgatctcgtc ggtcgccgac acgtcgtcac cgtcggtggc ctctccgtcg gatgcggccc
     3601 cgacgtcgtc gaccccatcg tcctgatgga gcagccgttc cttgatcacc ttcaggggga
     3661 ggtactcgtc gcgctggagc cggaggatcg acttcagccg gtcgacgtcg tggcggtaga
     3721 acttgcggta gccggacggc gtccgctcgg gctcgatcag cccctcggcc tcgaggaacc
     3781 ggatcttcga gatcgtgatg tccgggaact ccgtcttcac ggacacgagg acctcaccga
     3841 tcgactggta gttgcgcgtg ccggccatct ccacctcatc cccctgcctg gaagaacgtc
     3901 agcctgaagc gtccgatctg gacctggtcg cccgtggcca acttcgtctc gtcgacctg
//
LOCUS       DASUVE010000314         2456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1472557, whole genome shotgun sequence.
ACCESSION   DASUVE010000314 DASUVE010000000
VERSION     DASUVE010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472557"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1564)
                     /locus_tag="VF036_06790"
     CDS             complement(32..1564)
                     /locus_tag="VF036_06790"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEX6264970.1"
                     /translation="MRARAWRSAAIVAATSLTLLMAAPPASAAPFFTSVPLMITLADP
                     STSKATAIISVGDEVGDFFFEGVPDGIGAMPGTGGRVEVFVNHEQTTVPFDGLADFEM
                     ASVSHLTLDPSGGVLAGSVPVPDSAGFQRFCSSTMAGPADGLSSYLYLTGEEAPDALP
                     VPPGAPYGPDPAYAPENLRQSGYAVVLDPVTGEYTQVAGMGRHNHENAMVLPGGWDKI
                     AVVSGDDTFSAPSSQLYMYLAEDEDAIWADEGNLLAFRVTHKNGVRVAPGNAFNGAND
                     YGDVAAGDHLRGRFIRVPGWIARGTTATPPQDALERWSNRNNVFQFIRVEDTAYDPDV
                     PIGGNPVMYLADTGSSSAVPDPTTGRLTSGTGGAFGNGRIFQMTFAKHDPRRVVDFSI
                     VLDGNATANPLAPAPAMKNPDNLGISSDSLMIQEDVSSGFESRILRYDLNAGALSVVA
                     RVNTIGWESSGIVNASAFFGDGAWLLDVQAHSIFVDEETVGDVTLKREGGQLILLSVD
                     GS"
     gene            1682..>2456
                     /locus_tag="VF036_06795"
     CDS             1682..>2456
                     /locus_tag="VF036_06795"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX6264971.1"
                     /translation="MPDERRLLAMDDGVRIALSLHFPDEHPPPWPVILEAQPYRKDDF
                     SDSTATYRRLADEGGLAVCRADVRGTGSSEGVAEDEYSDREWLDHERTIAWLAQQEWS
                     NGNVGMWGTSYSGFNALQMAVRQPPALKAIIPIFATDRRYTDDVHYGGGARRGIDILD
                     YPTYMVAFNALPPVPFAYGEGWREEWRRRIDANEPWVLHWLEHQNENAYWRRGSSINA
                     YDRIEAATLVIAGHADGYRNMAFRAFERLRGPKQILFGPW"
BASE COUNT          404 a          836 c          827 g          389 t
ORIGIN      
        1 cgcccccgaa ggcggcgagc cccacatccg tctagctgcc gtccacgctg agcaggatca
       61 gctgtccgcc ctcccgtttg agggtgacat ctcccaccgt ctcctcgtcg acgaagatgg
      121 aatgtgcctg gacgtccagc agccaggctc cgtcgccgaa gaaggcggag gcgttgacga
      181 tgccgctgct ctcccacccg atcgtgttga cgcgtgcgac cacggacagg gcgccggcgt
      241 tcaggtcgta tcgcaggatc cgcgactcga agccagagct cacgtcctcc tggatcatca
      301 ggctgtcgga gctgatcccg aggttgtccg ggttcttcat cgccggtgcg ggagcgagcg
      361 gattcgccgt cgcgttcccg tcgaggacga tcgagaagtc gacgacgcgc ctggggtcgt
      421 gcttggcgaa cgtcatctgg aagatgcgtc cgttcccgaa ggcaccgccg gtgccgctcg
      481 tgagccgtcc cgtcgtcgga tccggcaccg cggagctgct gccggtgtcg gccagataca
      541 tcacggggtt gccgccgatc gggacatccg ggtcgtatgc cgtgtcctcg acgcggatga
      601 actggaagac gttgttccgg ttcgaccaac gttcgagcgc gtcctggggc ggcgtggccg
      661 tcgtacctct agcgatccac ccgggcaccc ggatgaagcg gccgcggagg tgatcaccgg
      721 ccgcgacatc gccgtagtcg ttcgcgccgt tgaacgcgtt cccgggagcg acccggacac
      781 cgttcttgtg ggtgacgcgg aacgccaaga ggttcccttc atcggcccag atcgcgtctt
      841 cgtcttccgc caagtacatg tacagctgcg aagacggggc ggagaaggtg tcgtcgcccg
      901 agacgaccgc gatcttgtcc catccaccgg gcagcaccat cgcgttctca tggttgtggc
      961 gccccatccc cgccacctgc gtgtactcgc ccgtcacggg atcgaggacc accgcatagc
     1021 cggactgccg gaggttctcc ggcgcgtacg cggggtcggg accgtagggg gctccaggag
     1081 ggacgggaag tgcgtccggg gcttcctcac ccgtcaggta gaggtaagaa gacaggccgt
     1141 cggccggacc ggccatcgtc gaggaacaga accgctggaa cccggcgctg tccggcaccg
     1201 gcaccgaccc ggcgaggacc ccgccgctcg gatcaagcgt caggtgcgac acggacgcca
     1261 tctcgaagtc ggcgagcccg tcgaacggca cggtggtctg ttcgtggttc acgaacacct
     1321 cgacgcgccc acccgtaccg ggcatcgcgc cgatcccatc gggcacgcct tcgaagaaga
     1381 agtcacccac ctcgtcgccg acgctgatga tcgcggtcgc cttcgacgtg ctcgggtcgg
     1441 cgagcgtgat catcaggggc accgacgtga agaaaggcgc tgccgacgcg ggtggcgcgg
     1501 ccatcagaag cgtgagagac gtcgccgcga cgatcgcggc gctcctccag gcgcgtgctc
     1561 gcatgcactt acctcctctt tcggggggac gtctccaccc tcccgcccga gcgagcaggt
     1621 cgtcaaatcc gctggtcgtg ggtcacggca ggcgagcagc ggccgagtac cgtttcccgc
     1681 catgccggac gagcggcggc tcctcgcgat ggacgacggg gtgaggatcg ccctctctct
     1741 gcacttcccg gacgagcatc cgcctccctg gcccgtgatc ctcgaggcgc agccgtaccg
     1801 gaaggacgac ttctcggact ccacggcgac ctaccggcgt ctggctgacg agggcggcct
     1861 cgccgtgtgc cgagccgacg tccgcggcac gggatcgtcg gagggtgtcg ccgaggacga
     1921 gtacagcgac cgcgagtggc tcgaccacga gaggacgatc gcgtggctcg cccaacagga
     1981 gtggtcgaac ggcaacgtcg ggatgtgggg cacctcgtac tcgggcttca atgcgctcca
     2041 gatggcggtc cggcagccgc cggcgctgaa ggcgatcatc ccgatcttcg ccaccgaccg
     2101 gcggtacacc gacgacgtcc actacggcgg cggcgcccgg cgcggcatcg acatcctcga
     2161 ctacccgacg tacatggtcg cgttcaacgc gctgccaccg gttccgttcg cctacggcga
     2221 gggatggcgc gaggagtggc gtcgacggat cgatgccaac gagccgtggg tgctgcactg
     2281 gctcgaacac cagaacgaga acgcgtactg gcgccgcggc tcgagcatca acgcgtacga
     2341 tcgcatcgaa gcggccacgc tcgtgatcgc cggacacgcc gacggctacc ggaacatggc
     2401 gttccgtgcc ttcgaacgac tccgcggtcc caagcagatc ctgttcggac cgtggg
//
LOCUS       DASUVE010000315         5383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1660475, whole genome shotgun sequence.
ACCESSION   DASUVE010000315 DASUVE010000000
VERSION     DASUVE010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660475"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VF036_06800"
     CDS             complement(<1..261)
                     /locus_tag="VF036_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6264972.1"
                     /translation="MRKWVLLLAAIALITAACSEETPPTTGESPTGSVADPEAAAECT
                     AGHAADLLETETLTVGTDFDFMFPPWILDRDPESGKGFEAALT"
     gene            complement(310..1116)
                     /locus_tag="VF036_06805"
     CDS             complement(310..1116)
                     /locus_tag="VF036_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6264973.1"
                     /translation="MSAVPQVARQFRFTNKAFWRNPASAFFTFAFPLMFLVIFTSLLG
                     NNELQLQGVELSTTTYYVGAMAAFGVISACFTNIAIQTTFNRDAGILKRLRGTPLPAG
                     AYLTSRVLHAMVVGAILVVITLLFGKVVYDSPLPTGGPLLEFIASFLVGALSFAALAL
                     ALTALVPNADAAPPVVNAVVLPLLFLSGIFFPLDDGAPTWMKTVADIFPVKHFADAMR
                     AGFLGNVTARTPVGTVRAFPFDWWDIAIIAAWGIVGLVLAARFFSWEPRR"
     gene            complement(1113..2024)
                     /locus_tag="VF036_06810"
     CDS             complement(1113..2024)
                     /locus_tag="VF036_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6264974.1"
                     /translation="MTERVIDVRGLRKSYGDVEAVRGIDVHVDRGEVFALLGPNGAGK
                     TTTVEILEGYRERTDGEISVLGHDPARAEAELKGRVGIVLQSTGVDPYLTVRETVDLY
                     AGYYPSPRDIDEVIDLVGLVEKRSTRVSKLSGGQQRRLDVAIALAGDPELLFLDEPTT
                     GFDPNARRHAWEIVKNLAGIGKTIFLTTHFMDEAQYLASRAAVIRKGEIVAEGSPGTL
                     GGRDRMQTRVRFRVPDGQAPPPDLTGTTTLVDGTLELRTDDTTKSVHDLTAWALGRGF
                     SFDGLEVTQPSLEDVYLELTGGEESEG"
     gene            complement(2032..2403)
                     /locus_tag="VF036_06815"
     CDS             complement(2032..2403)
                     /locus_tag="VF036_06815"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 family protein"
                     /protein_id="HEX6264975.1"
                     /translation="MTEERLFSIGDADAELEELRVRLPRLREARRRLIDTGERITSAV
                     QIDGGGTEGSDWFRAQETLKRELLWLADRGILLRDPDTGLVDFPAERAGERVFLCWRL
                     GEERVGWYHSERSGFSSRKPL"
     gene            complement(2400..3104)
                     /locus_tag="VF036_06820"
     CDS             complement(2400..3104)
                     /locus_tag="VF036_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6264976.1"
                     /translation="MRFRAVFFDVGETLVHVDPSFADLFVTVLAGAGHERSVDDVREA
                     SAHVYARFSEAARDGSLWTTSPERSRAFWTSVYQRMLEELGVADGDGLATSLHGEFTR
                     MENYVLFDDVLPTLATLREAGVLLGVVSNFEAWLEDWFGVHELFELFPVRVISGIEGV
                     EKPDERIFRLALDRASVSAEESAYVGDNPEFDVDPPAALGMFPVLVDRRGRFPDHEGE
                     RITDLRELPAILGATS"
     gene            complement(3161..3763)
                     /gene="coaE"
                     /locus_tag="VF036_06825"
     CDS             complement(3161..3763)
                     /gene="coaE"
                     /locus_tag="VF036_06825"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015576761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX6264977.1"
                     /translation="MLLVGLTGGIGSGKSTVARLLERRGAVVIDADHLAREAVARGTS
                     GFDRVVDTFGPGVLKPDGDLDRVRLAAIIFAHPERRAALESIVHPEVARRFGERVEEL
                     RDSGRVVVYVSPLLVELGLAPAFDVVVVVTASPHLRVSRVASDRDLSPEDVRARMAAQ
                     ATDEQRMEVADVLVDNDGSLKELEPQIDRLWSDLEDRARA"
     gene            complement(3774..5375)
                     /gene="rpsA"
                     /locus_tag="VF036_06830"
     CDS             complement(3774..5375)
                     /gene="rpsA"
                     /locus_tag="VF036_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007322920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX6264978.1"
                     /translation="MSETETTTESAPDGTVETSEPIVDEAAATETASDVAPPATPTDE
                     APLVATPEPEGEDYTPEELVRAMEASLRDFKDGDIMEGVVVKIDRDEILLDIGYKSEG
                     VIPSKELSIRHDVDPNEIVKVGDRIEALVLQKEDKEGRLILSKKRAQYERAWGRIEET
                     MASGQTIKGPVIEVVKGGLILDIGLRGFLPASLVDLRRVRDLQPYIGTELECKIIELD
                     RNRNNVVLSRRAFLEESQSEGRKKFLENLEKGERRKGTVSSIVNFGAFVDLGGVDGLV
                     HVSELSWKHVDHPSEVVTVGQEVEVQVLDVELDRERVSLSLKATQEDPWKEFERLHKA
                     GEVIAGQVTKLVPFGAFVRVAQGIEGLVHISELSHEHVDTPESVLSVGDEVQVKVVDV
                     DVSRRRVSLSMRQVTPAPAKTTEIEVEPEQPERAPAEPLVQTVQIPQAVLDAVAEAET
                     DEPAATAAPTVEPAAVEPTAEPEAAPVPAAEAAPDEQPEAAAAPEVAAQPPPAKDAPE
                     EPASDEEVSLEAILEDLKRREGRSE"
BASE COUNT          815 a         1903 c         1827 g          838 t
ORIGIN      
        1 ggtcaacgcg gcctcgaacc ccttcccgct ctccgggtcg cgatcgagga tccacggggg
       61 gaacatgaag tcgaagtccg tcccgacggt caacgtctcg gtttcgagga gatccgcggc
      121 gtggcccgcc gtgcattcgg cggccgcttc cggatcggca acggacccgg tggggctttc
      181 gcccgtggtg ggtggcgtct cctcggagca agccgccgtg atcagcgcga tcgccgcgag
      241 cagcagtacc cacttccgca cggaggcacc ccctcgttcc cgtggaagcg cggagcctag
      301 cacagccccc tagcggcgcg gctcccagct gaagaaacgc gcggcaagga cgagtccgac
      361 gatcccccag gccgcgatga tcgcgatgtc ccaccagtcg aacgggaacg cgcgcaccgt
      421 gccgaccggg gttcgggcag tcacgttccc gaggaacccg gctcgcatcg cgtccgcgaa
      481 atgcttcacc gggaagatgt cggcgaccgt cttcatccac gtcggcgcgc cgtcgtcgag
      541 cgggaagaag atgccagaga ggaacagcag gggcaggacg accgcgttca ccacgggcgg
      601 cgcggcatcg gcgttcggaa cgagggcggt gagcgccagc gcgagcgccg cgaacgagag
      661 ggcgccgacc aggaaggacg cgatgaactc gaggagcggg ccaccggtcg gcagcggcga
      721 gtcgtagacg accttcccga acaggagcgt gatcaccacc aggatcgcac cgacgaccat
      781 cgcgtggagc acccggctcg tgaggtaggc cccggcgggg agcggggtgc cgcgcaggcg
      841 cttgaggatc cccgcgtcac ggttgaacgt cgtctggatc gcgatgttgg tgaagcaggc
      901 gctgatcacg ccgaacgctg ccatcgctcc cacgtagtac gtggtcgtcg agagttcgac
      961 gccctggagc tgcagctcgt tgttccccaa caacgacgtg aagatcacga ggaacatcag
     1021 cgggaacgcg aacgtgaaga acgcggaggc ggggttccgc cagaacgctt tgttcgtgaa
     1081 ccggaactgg cgtgcgacct gaggaacagc gctcatcctt cgctctcctc cccgccggtc
     1141 agctccaggt acacgtcttc gaggctcggc tgcgtgactt ccagcccgtc gaacgagaac
     1201 ccgcgaccga gggcccatgc cgtgaggtcg tggacgctct tcgtcgtgtc atccgtccgc
     1261 agctcgagcg tgccgtcgac gagcgtcgtc gtgcccgtca gatccggcgg tggcgcctgg
     1321 ccgtccggga cccggaaccg caccctggtc tgcatccgat ccctgccgcc gagcgtccct
     1381 gggctcccct cggccacgat ctcgcccttg cggatcacgg ccgcgcggct cgcgaggtac
     1441 tgggcctcgt ccatgaagtg cgtggtgagg aagatcgtct tgccgatgcc cgccaggttc
     1501 ttcacgatct cccacgcgtg acgccgcgcg ttcggatcga agccggtcgt gggctcgtcg
     1561 aggaagagca actctgggtc cccggcgagg gcgatcgcga cgtcgaggcg gcgctgctga
     1621 ccgcccgaga gcttgctcac ccgggtggat cgcttctcga cgaggcccac caggtcgatc
     1681 acctcgtcga tgtccctcgg cgacgggtag tagccggcat agaggtcgac cgtctcgcga
     1741 acggtgaggt aggggtcgac gcccgtggat tggagaacga tgccgacccg ccccttgagc
     1801 tcagcctccg cacgcgccgg gtcgtgtccc aggacagaga tctcgccgtc ggtccgctcc
     1861 cggtagccct cgaggatctc gaccgtggtc gtcttgccgg cgccgttggg accgaggagg
     1921 gcgaagacct cgccgcgatc gacgtggacg tcgatgccgc ggaccgcctc aacgtcgccg
     1981 taggacttgc ggagaccccg gacgtcgatc acccgctcgg tcatcgcgcg atcacagggg
     2041 cttcctgctc gagaacccgg accgttcgga gtggtaccac ccgactcgtt cttccccgag
     2101 ccgccaacag aggaacacgc gctccccggc gcgctcggcc gggaagtcta cgagccccgt
     2161 gtccggatcc ctcaggagga tgcctcggtc cgcgagccac aggagctccc gcttgagcgt
     2221 ctcctgcgcc cggaaccagt cgctgccctc ggtgcctcct ccatcgatct ggacggccga
     2281 ggtgatgcgt tcgccggtgt cgatgagtcg ccgtcgagcc tccctgagcc tcgggagccg
     2341 gacgcggagc tcctccagct cggcgtcggc gtcgccgatc gagaacagcc gctcctcggt
     2401 cacgacgtcg ctccgaggat cgcggggagc tcccggagat ccgtgatgcg ctcgccctcg
     2461 tggtcgggga accggccccg gcggtccacg aggaccggga acatcccgag ggccgccggc
     2521 gggtcgacgt cgaactcggg gttgtcgccg acgtaggcgg attcctccgc cgacacggac
     2581 gcccggtcca gggcgagccg gaagatccgc tcgtcgggct tctccacgcc ctcgatcccg
     2641 gagatcacac ggaccgggaa cagctcgaag agctcgtgga cgccgaacca gtcctcgagc
     2701 catgcctcga agttcgacac cacgccgagg agcacacccg cctcgcggag cgtcgccagc
     2761 gtcgggagca cgtcgtcgaa caggacgtag ttctccatcc gggtgaactc gccgtgcagc
     2821 gaggtcgcga gtccgtcacc gtcggcgacg ccgagctcct cgagcatccg ctgatagacc
     2881 gaggtccaga aggcgcggga tcgctcgggc gacgtcgtcc acaacgagcc gtcccgcgcc
     2941 gcctcggaga accgtgcgta gacgtgtgcc gaggcctccc gcacgtcgtc cacggaccgc
     3001 tcgtggcccg cgccggcgag cacggtcacg aagaggtcgg cgaaggaagg atcgacgtgc
     3061 acgagggtct caccgacgtc gaagaagacg gcgcggaacc gcatgccccg aggataggac
     3121 ttctgatccc tcagccgatc gcccgcgcgg atccaccggc tcacgcgcgg gcccggtcct
     3181 cgagatcgct ccagagccgg tcgatctgcg gctccagctc cttgagcgac ccgtcgttgt
     3241 ccaccaggac gtccgccacc tccatccgct gctcgtccgt cgcctgggca gccatccgag
     3301 cgcgcacgtc ctcgggcgag aggtcgcgat ccgaggccac ccgcgagacc cggaggtggg
     3361 gtgacgccgt gacgaccacg accacgtcga aggcgggtgc gagtcccagc tcgacgagga
     3421 gcggactgac gtacacgacc acacggccgc tgtctcggag ctcctcgacg cgctcgccga
     3481 accgcctcgc gacctcgggg tggacgatcg actcgagcgc ggcccgccgc tcggggtggg
     3541 cgaagatgat cgcggcgagc cgtacccggt cgagatcccc gtcgggcttc aggacgcccg
     3601 gaccgaaggt gtcgacgacg cgatcgaacc cgctcgtgcc tcgcgccacg gcctcgcgtg
     3661 cgaggtggtc cgcatcgatc acgacggccc cacgccgctc gagcaggcgg gcgaccgtgg
     3721 acttgccgga gccgatcccg cccgtcaggc cgaccaggag cacgatcccg cccctactcg
     3781 gatctcccct cgcggcgctt cagatcctcg aggatcgcct cgagcgagac ctcttcgtcc
     3841 gatgcgggct cctcgggggc atccttcgcc gggggcggtt gcgcggcgac ctcgggcgct
     3901 gccgctgcct ccggctgctc gtcgggtgct gcctcggccg ccggcaccgg cgcggcttcc
     3961 ggctccgccg tgggctcgac cgccgccggt tccacggtgg gtgccgcggt ggccgccggc
     4021 tcgtccgtct ccgcctccgc gacggcgtcg agcaccgcct gcgggatctg cacggtctgg
     4081 acgaggggct cggcgggagc acgctcgggc tgctcgggct cgacctcgat ctccgtggtc
     4141 ttggccggag cgggggtgac ttggcgcatc gagaggctca cccgccggcg cgagacgtcc
     4201 acgtcgacga ccttcacctg cacctcgtcg cccacggaca gcaccgactc cggcgtgtcg
     4261 acgtgctcgt gggagagctc cgagatgtgg accagtccct cgatcccctg ggcgaccctg
     4321 acgaacgcgc cgaacgggac gagcttcgtc acctgaccgg cgatcacctc gcccgccttg
     4381 tgcagccgct cgaactcctt ccaggggtcc tcctgcgtcg ccttgaggga gagcgagaca
     4441 cgctcgcggt cgagctccac gtccaggacc tggacctcga cttcctggcc gaccgtgacg
     4501 acctcgctcg ggtggtcgac gtgcttccac gagagctccg acacgtggac cagcccgtcc
     4561 accccgccga ggtccacgaa cgctccgaag ttcacgatcg aggagacggt gcccttgcgg
     4621 cgctcgccct tctccaggtt ctcgaggaac ttcttacggc cctccgactg cgattcctcg
     4681 aggaaggcgc gccgggacag cacgacgttg ttgcggttgc ggtcgagttc gatgatcttg
     4741 cactcgagct cggtgccgat gtacggctgg aggtcgcgca cccgccggag gtcgacgagc
     4801 gaggcgggga ggaacccccg gaggccgatg tcgaggatca ggccgccctt gaccacctcg
     4861 atcaccggtc ccttgatcgt ttggcccgac gccatcgtct cctcgatccg gccccaggcg
     4921 cgctcgtact gcgcgcgctt cttcgacagg atcagacggc cctccttgtc ctccttctgc
     4981 aggacgaggg cctcgatccg atcccccacc ttgacgatct cgttcgggtc gacgtcgtgg
     5041 cggatcgaca gctccttgga cgggatcacg ccctcggact tgtagccgat gtcgaggagg
     5101 atctcgtcgc gatcgatctt gacgacgacg ccctccatga tgtcgccgtc cttgaagtcg
     5161 cggagcgatg cctccatcgc gcggacgagc tcctcgggcg tgtagtcctc gccctccggc
     5221 tcgggcgtgg caacgagggg tgcctcgtcc gtcggtgtgg cgggcggggc gacgtcgctg
     5281 gcggtctcgg tggccgctgc ctcgtcgacg atcggctcag aggtctcgac ggttccgtcg
     5341 ggggcgctct cggtggtggt ctcggtttcg ctcatacggt ggt
//
LOCUS       DASUVE010000316         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_907435, whole genome shotgun sequence.
ACCESSION   DASUVE010000316 DASUVE010000000
VERSION     DASUVE010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907435"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VF036_06835"
     CDS             complement(<1..394)
                     /locus_tag="VF036_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX6264979.1"
                     /translation="MTMVRAAVIQAHANMPKDDAVDKHVELIAKAAADGAEVTCLQEI
                     FHGPYFCAEQDPKWYGTAEPEDGPLVTRMREVAKIHNMVLIVPWFEEAQTGVYYNSAV
                     VIERDGSIAGKYRKTHIPHVGPCFWEKFY"
     gene            complement(435..1436)
                     /locus_tag="VF036_06840"
     CDS             complement(435..1436)
                     /locus_tag="VF036_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX6264980.1"
                     /translation="MLSFGVTFMLDPPVSRTIEWAKLAEAQGFDAVWAWDSHVLWQEF
                     YTTFALVAANTERVRLGPCVTNPATRDMTVTASAMATLQEVSGGRMDIGMGRGDSARR
                     VIGQKPVTVEHMEHACRFIKDLAEGREVDNEGTPLQLKWSEGHRLPVWVAAYGPKALR
                     ACGRVADGLIMQLADPFILGWSLAYVREGAEEAGRAFGDIQIQVAAPGYLSEDLAMAR
                     DQVRWFPALVSNHVVDLVKRYSDADLPQALTDYIEARDHYDYADHGRTGAEHADFVTD
                     EVVDRFCVLGTADACIGKLRELEALGVTQFNIYSMQEDPGPAGIIEGFGKDVLPAFR"
     gene            1687..>2216
                     /locus_tag="VF036_06845"
     CDS             1687..>2216
                     /locus_tag="VF036_06845"
                     /inference="COORDINATES: protein motif:HMM:NF014827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX6264981.1"
                     /translation="MTDDASGHAVSAERLFDAVQGHLEVAAERLRLEDGARKVLSTHR
                     REVAVSVRVPRDDGSFDVYPGWRIQHNGARGPFKGGLRFHPNASRDEFRCFSALMTWK
                     TALLDVPFGGAKGGVQVDPKSLSVAELERLCRSYLSSIELVIGPYRDIPAPDVNTGPR
                     EMAWMYDEYSRSRGDSP"
BASE COUNT          336 a          768 c          722 g          390 t
ORIGIN      
        1 aatagaactt ctcccagaag cagggcccga cgtgggggat gtgggtcttg cggtacttgc
       61 ccgcgatcga cccgtcccgc tcgatcacga ccgcggagtt gtagtacacg ccggtctgcg
      121 cctcctcgaa ccacgggacg atgaggacca tgttgtggat cttggcgacc tcgcgcatcc
      181 gcgtgacgag cggaccgtcc tccggttcgg cggtcccgta ccacttcgga tcctgctccg
      241 cgcagaagta gggcccgtgg aagatctcct gcaggcatgt gacctccgcg ccgtcggcag
      301 ccgccttcgc gatcagctcg acgtgcttgt cgaccgcgtc gtccttgggc atgttcgcgt
      361 gcgcctggat cacggccgcc cggaccatcg tcatcgttcc tgcctccctc tgttctcaga
      421 cgtccagttc cgcatcagcg gaacgccggt agcacgtcct tcccgaaccc ttcgatgatc
      481 ccggccggac cgggatcctc ctgcatcgaa tagatgttga actgcgtcac gccaagcgcc
      541 tcgagttcgc gcagcttccc gatgcatgcg tcggccgtgc ccagaacgca gaaccggtcc
      601 acgacctcgt cggtcacgaa gtctgcgtgc tcggcaccgg tccgtccgtg gtcggcgtag
      661 tcgtagtgat ctcgagcctc gatgtagtcg gtcaacgctt gaggaaggtc ggcgtccgag
      721 taccgcttga cgaggtccac gacgtggttc gagacgagag cggggaacca ccgcacctgg
      781 tcgcgggcca tcgcgaggtc ctcggagaga taccccggtg ccgccacctg gatctggatg
      841 tccccgaagg ctcgccctgc ctcctcggcc ccttctcgga cgtaggcgag cgaccatccg
      901 aggatgaacg ggtccgcgag ctgcatgatc aggccgtcgg caacccgccc gcaggcgcgg
      961 agtgccttcg gcccgtacgc cgccacccac accggcaggc gatggccctc gctccatttc
     1021 agctggagcg gggtgccctc attgtcgacc tcacgcccct ccgcgagatc cttgatgaac
     1081 cgacaggcgt gctccatgtg ctcgaccgtg accggcttct gtccgatcac gcgccgcgcg
     1141 ctgtcacctc gacccatccc gatatccatc cgccccccgg acacttcctg gagcgtcgcc
     1201 atcgcgctcg cggtcaccgt catgtcgcgg gtcgcggggt tcgtgacgca cggcccgagc
     1261 cggacgcgtt ccgtgttcgc cgcgacgagt gcgaacgtcg tgtagaactc ctgccagagc
     1321 acgtgggaat cccaggccca cacggcgtcg aacccctgcg cttcggcgag cttggcccac
     1381 tcgatcgtcc tggagaccgg ggggtcgagc atgaacgtca caccgaagga gagcacctgc
     1441 tcgtcgactt cctctctcgg ccgccgttcg ggccggttgc tgataggcgc atcgtagggg
     1501 cgcgtcagcg agcgagcaat cggacgttcg ggtgtgaggg cttcgtgatc gggcatggcc
     1561 cccgatggac gagggcccgg acccgtgcgt gacaggacga ccatgcggaa cccttgaccc
     1621 ggtcgaagct cggggatccc gcacgccggt acgcgcgcgg gtccgccgca tgaaagactc
     1681 gcgggcatga cggacgacgc gtccggccac gccgtctccg cggaacgctt gttcgacgcc
     1741 gtccaaggtc atctcgaggt cgccgcggag cggctgcgcc tcgaggacgg agcccgcaag
     1801 gtcctgtcga cgcaccgtcg ggaggtggcg gtgtcggttc gtgttccccg cgacgacggc
     1861 tccttcgacg tgtacccggg gtggcggatc cagcacaacg gtgcgcgcgg tccgttcaag
     1921 ggcgggctgc ggttccatcc gaacgcctct cgggacgagt tccggtgctt ctctgccctg
     1981 atgacgtgga agaccgcgct gctcgacgtc ccgttcggag gtgcgaaagg cggcgtccag
     2041 gtggacccga agagcctctc ggtggcggag ctcgagcgac tgtgccggtc ctacctctcg
     2101 tcgatcgagc tcgtgatcgg gccctatcgc gacatccccg cgcccgacgt gaacaccggc
     2161 ccgcgcgaga tggcgtggat gtacgacgag tactcgcgct ctcgtggtga ctcgcc
//
LOCUS       DASUVE010000317         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_721215, whole genome shotgun sequence.
ACCESSION   DASUVE010000317 DASUVE010000000
VERSION     DASUVE010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721215"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /locus_tag="VF036_06850"
     CDS             <1..1289
                     /locus_tag="VF036_06850"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6264982.1"
                     /translation="GGRPVPRVTDPTPRRDTRWPIKRVLYLMLENRSFNNLFGKFPGV
                     RAGTTVGVENGVEKPLIRCPEWLPGDIPHDLAAHRTCVNNGEMDGFGTGIHGSTYGYT
                     IFDEDQIPNYWHWAREYSLSDSFFASAAGPSYPNHYYFIAGSSGGVADNPENIRTRPL
                     EDGGLFKSWGCDVVGDDVFVLVTDEAGNLTKHNTCFEFPTVGEQLTQDGIDWAYYSAV
                     PGQPGYFWNAYNGIANVFHTDLWPEHIRSVDTLLDDIDANALPPVTWVTPRFQLSDHP
                     PFSTGHAHNWVTKIVNKVMRSDMWEHTAIFLTWDEWGGFYDPVVPPSVDHLGLGIRVP
                     LLTISPYTRRGVIDDERGEFSTPLRFISDNWGLEPLTDRIRNTHNLEHVFDFGAKPRA
                     PEFGRRQAPTTSSIWRFPSDTYTGWEPGTVPVEDPL"
     gene            complement(1293..1439)
                     /locus_tag="VF036_06855"
     CDS             complement(1293..1439)
                     /locus_tag="VF036_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016826775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264983.1"
                     /translation="MKRERIEKPKPRRPREFEMIIPLDPRDPDVVRAKKGWVWRYADR
                     RWAV"
     gene            complement(1604..>2671)
                     /locus_tag="VF036_06860"
     CDS             complement(1604..>2671)
                     /locus_tag="VF036_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX6264984.1"
                     /translation="AGVLDPGLVAENPLDAWGTGIDADRIYVESFQALHDDPETAVVA
                     FVVDLTRQGEPYDVGYLQVARDVWDRTTKPFCVISNLPATIADDEVGVLREAGIPVLE
                     GTVTGLRALRHLLGDATGRSRLAVDAPPASSPGIEAEWRGRFAEAADVSEVAALGLLA
                     AYNIPVVDALPARSLDEAERAADRIGYPVAIKTAVAGILHKSEVRGVRVGIPDVDELR
                     ETYWDLADRLGPDVTVAAMAPPGVEVALGIVRDPTFGPLVLVAAGGVLVVVLHDRKLA
                     LPPIDVDAARRLIDGLAVRPLLDGVRGAPPSDVDALARAVSRLSVLALELGDRIAELD
                     VNPVIVSPSGCVAVDALVIPG"
BASE COUNT          439 a          950 c          891 g          391 t
ORIGIN      
        1 ccggcgggcg ccccgtcccg cgcgtgacgg acccgacccc gaggcgcgac acccgctggc
       61 cgatcaagcg cgtcctgtac ctgatgctcg agaaccggag cttcaacaac ctgttcggga
      121 agttccccgg cgtcagggcg gggaccacgg tcggcgtcga gaacggcgtc gagaaaccgc
      181 tgatccgctg cccggagtgg ctgccgggcg atatcccgca cgatctcgcc gcgcaccgga
      241 cgtgcgtgaa caacggagag atggacgggt tcggcacggg gatccatggc tcgacgtacg
      301 gctacacgat cttcgacgag gaccagatcc cgaactactg gcactgggcg cgtgagtaca
      361 gcctctcgga cagcttcttc gcctccgcag cgggtcccag ctacccgaac cactactact
      421 tcatcgcggg gtcctcgggc ggcgtcgccg acaacccgga gaacatccgg acgaggccac
      481 tcgaggatgg agggctcttc aagagctggg gctgcgacgt ggtgggggac gacgtgttcg
      541 tcctggtgac ggacgaggcc gggaatctga cgaagcacaa cacgtgcttc gagttcccta
      601 cggtcggcga gcagctcacc caggacggca tcgactgggc gtactattcg gcggtccctg
      661 ggcagcccgg ctacttctgg aacgcgtaca acggcatcgc gaacgtcttc cacacggacc
      721 tgtggcccga gcacatccga tcggtcgaca cactcctcga cgacatcgac gcgaacgccc
      781 tcccgcccgt cacctgggtg accccacggt tccagctctc cgaccatccg ccgttctcca
      841 cgggccacgc ccacaactgg gtcacgaaga tcgtgaacaa ggtgatgcgc tccgacatgt
      901 gggagcacac ggcgatcttc ctgacgtggg acgagtgggg aggcttctac gatcccgtcg
      961 ttccgccgtc cgtggatcac ctgggcctgg gcatccgcgt cccgttgctc acgatctcgc
     1021 cgtacacgcg ccggggcgtg atcgacgacg agcgaggcga gttctcgacg ccgctgcggt
     1081 tcatctcgga caactggggg ctagagccgc tcaccgatcg gatccgcaac acccacaacc
     1141 tcgagcacgt cttcgacttc ggggcgaaac cgcgtgctcc ggagttcggt cgccggcagg
     1201 cgcccacgac gtcatccatc tggagattcc cgagtgatac gtacacgggc tgggaaccgg
     1261 gaacggttcc cgtggaggac ccgctctagc cgctagacgg cccagcggcg atccgcgtag
     1321 cgccagaccc agcccttctt cgcgcgaacg acatcgggat cgcggggatc gagcggaatg
     1381 atcatctcga actcacgcgg gcggcggggc ttcggcttct cgatccgttc gcgcttcacg
     1441 gccgctcacc tcccgtgctc cgccggctcc gtcgcgagtg gttcgcatcc ggtggctcgg
     1501 cggatcgtgt ctcaggcata ccccctcgat gagagttgta tgccaagcct gggaagcggg
     1561 accatgaccg gcatcacgtt tcgggtgtga ccttcgtcgg gggtcagccc gggatcacga
     1621 gcgcgtcgac ggcgacgcag ccagagggcg acacgatcac cgggttgacg tcgagctcgg
     1681 cgattcgatc gccgagctcg agcgccagga cggagagacg ggagaccgcg cgcgcgagcg
     1741 cgtcgacgtc ggacggtggc gctccgcgca cgccgtcgag gaggggacgg accgcgaggc
     1801 cgtcgatcag gcggcgggcg gcgtccacat cgatcggagg gagcgcgagc ttccggtcgt
     1861 gcagaaccac caccaggacg ccgcccgccg cgaccagcac gagcgggccg aacgtcgggt
     1921 cgcgcacgat gccgagcgcc acctccacgc ccggcggcgc catcgccgcc accgtcacgt
     1981 ccgggccgag ccggtccgcc aggtcccagt aggtctcgcg gagttcatcc acatccggga
     2041 tgcccacgcg gacgccccgt acctcggact tgtgcaggat gccagccacc gccgtcttga
     2101 tcgcgacggg atagccgatc cggtcggccg cccgctccgc ctcgtcgagc gaccgggccg
     2161 ggagcgcgtc gacgacgggg atgttgtacg cggccagcag gcccagcgcc gccacctcag
     2221 agacgtcggc ggcctcggcg aatcgtccgc gccactcggc ttcgatcccc ggtgaggacg
     2281 cgggcggggc atccacggcc agccggctcc gccccgtggc atctccgagc aggtgcctca
     2341 gcgcccggag gccggtcacg gtgccctcga gcacggggat acccgcctcg cggaggacgc
     2401 cgacctcgtc gtcggcgatc gtcgccggga ggttcgagat cacgcagaac ggcttcgtcg
     2461 tccgatccca cacgtcgcgc gccacctgca ggtaccccac gtcgtacggc tcgccctgcc
     2521 gcgtcaggtc caccacgaac gcgaccaccg cggtctccgg atcgtcgtgg agcgcctgga
     2581 agctctcgac gtagatccgg tccgcgtcga tcccggtgcc ccaagcgtcc agcgggttct
     2641 cggccacgag cccgggatcc agcacgccgg c
//
LOCUS       DASUVE010000318         3452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_344084, whole genome shotgun sequence.
ACCESSION   DASUVE010000318 DASUVE010000000
VERSION     DASUVE010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344084"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /gene="nth"
                     /locus_tag="VF036_06865"
     CDS             complement(<1..569)
                     /gene="nth"
                     /locus_tag="VF036_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX6264985.1"
                     /translation="MSAKQQLTRDDPIEERAPAIIRRLSEAYPDAKVALHFTNPLECL
                     VATVLSAQSTDETVNRVTETLFQKYRTAEDYLRVSEDELKADIHATGFFNQKAISIRE
                     ACRRIVEEYGGEVPGTMEDLITLRGVARKTANIVLGNSFGIVEGIAVDTHVNRLAHRL
                     GFSEQKNPDKIEQDLMRLVPKEQWFDFTYV"
     gene            complement(875..1198)
                     /locus_tag="VF036_06870"
     CDS             complement(875..1198)
                     /locus_tag="VF036_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX6264986.1"
                     /translation="MNELDGGTIYNAQLLFGPDGELVRKHRKLMPTGGERMVWGFGDG
                     SDLDPVETPFGRVVGLICWENLMPLARTAIWRRASTSGRPPRGTTTRTGSRTSGTSPA
                     RVACT"
     gene            complement(1221..1496)
                     /locus_tag="VF036_06875"
                     /pseudo
     CDS             complement(1221..1496)
                     /locus_tag="VF036_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
     gene            complement(1496..2107)
                     /locus_tag="VF036_06880"
     CDS             complement(1496..2107)
                     /locus_tag="VF036_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6264987.1"
                     /translation="MDEMYHDGNRELQDRFDTRRLADRIEDVLVHDTFTEHDRAFVET
                     RDMFFLATADEDGKPNVSYKGGDPGFIRVVDEHTLAFPNYNGNGMYLSAGNLLRNPHV
                     GILFIDFERRNRMRVNGEASVDFDDVLRSQWPEAQFVVRVRAREIFPNCPRYVHRYQK
                     VAPSEFVPREDCETPVPSWKRADWAVDVLPEGDPALDPSATVV"
     gene            complement(2120..2833)
                     /locus_tag="VF036_06885"
     CDS             complement(2120..2833)
                     /locus_tag="VF036_06885"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX6264988.1"
                     /translation="MTWWSLLGSGEFQPWSEPVDRRLVEEADGDGRVLILPTASAPEG
                     KDVFERWGSLGLAHFSRLEIAAEVLELKTREDTDDPRLIERLEGASAVYLSGGNPAYL
                     ASTLTESDFLDALVKQLDRGMAYAGCSAGVACLNEVTFDSDTTDVDQIFKQGLNLIPR
                     TLFAPHWDMVDTWMPGAAEFLVESAPEGSTFIGMDEDTAMVGDGSAWEVLGKSGIHVR
                     IDRIWTTYRDGERFDLALV"
     gene            complement(2830..>3452)
                     /locus_tag="VF036_06890"
     CDS             complement(2830..>3452)
                     /locus_tag="VF036_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX6264989.1"
                     /translation="AFDPHKWLYTPQSGGCVIVRDMELMRRAFDVDYVAYVVKDEEHT
                     DWGIDLGRNSPNFSRGFWALKVWVSLLAHGRTAYGRRISHDAALARYLGARAEESDEF
                     ELMTPVGLSITCFRYVPADLSASGAGGNEPDRDAYLDLLNNRLMTEIQLDGRVYCSNA
                     VLGDRFCLRSCIVNYRTEAEDMDALLDVASELGAKLDVELRPEALR"
BASE COUNT          542 a         1212 c         1107 g          591 t
ORIGIN      
        1 acgtacgtga agtcgaacca ctgctctttc gggaccaacc gcatgagatc ctgctcgatc
       61 ttgtcggggt tcttctgttc ggagaacccg agccgatgcg ctaagcggtt cacgtgcgtg
      121 tcgaccgcga tcccctcgac gatcccgaac gagttcccga ggacgatgtt cgcggtcttc
      181 ctcgccacgc ctcggagcgt gatcaggtcc tccatcgtgc cgggcacctc accgccgtac
      241 tcctcgacga tccggcggca tgcctcgcgg atcgagatcg ccttctgatt gaagaacccg
      301 gtcgcgtgga tgtcagcctt cagctcgtcc tcggacacgc gcaggtagtc ctcggcggtc
      361 cggtacttct ggaacagcgt ctcggtgacc ctgttcaccg tctcgtcggt ggactgagca
      421 gagaggacgg tcgcgaccag gcactcgagc gggttcgtga agtgcagggc gaccttcgcg
      481 tcggggtagg cctcggagag gcgccggatg atcgcggggg cgcgctcctc gatcggatcg
      541 tcgcgggtta gctgctgctt ggcggacacg cccggatgct actcggacgt caccgaccgc
      601 ttcggcgcgt cgttcacgac cagcgtgaac acgtctggcc ggccgtagtg gccgacggga
      661 tcgaacttgt accgcgccgc cctcccgacc tcggcatcca tctcagcgga gaggatgccc
      721 tcttcctcca cgagcgggcc cgccaggagc tgaccctcgg gatcgacgat cgcggaccat
      781 cccgggttcg cccagtcctc ctcgccgccc cacagctcac gacccgggat gtcgtcgggg
      841 atgtcggagc cgcggagcaa cgtgcagacg ccgatcacgt acacgcgacc ctcgcgggcg
      901 acgtgccgga ggttcgtgat ccagttctcg tcgttgtccc acgtgggggc cgcccagatg
      961 tcgacgccct tcgccagatc gcggtccgcg cgagcggcat caggttctcc cagcagatca
     1021 acccgacgac gcgcccgaag ggcgtctcga ccgggtcgag gtcggacccg tcgccgaacc
     1081 cccataccat ccgctcgccg ccggtcggca tcagcttccg gtgcttcctc acgagctcgc
     1141 cgtcgggacc gaacagcagc tgggcgttgt agatcgtgcc accgtcgagc tcgttcacgg
     1201 accgagaggc aggccttcgc ccgcttcgca gccttcccga tctcgtcggt cgccgacgac
     1261 ggcacctgga ccgattcctc gcggaggcgt gccgtgagcg cctcgaacgg acccacccag
     1321 gcggccgcgc gccagaccca gtccggatag gtcgggatga aggtctccgg gaacacgatc
     1381 aggcgcgctc cctggccggc ggcttccttc gtcaggcgca ccaccttctg cacggtggca
     1441 tcgcggtcga ggaactccgg cgttgcctgg accgcagcga ccgtgaccgt ctccatcaga
     1501 cgaccgtcgc gctcgggtcg agggcggggt cgccttcggg cagcacgtcg acggcccagt
     1561 cggcgcgctt ccacgacggc accggggtct cgcagtcctc ccgcggaacg aattctgacg
     1621 gagcgacctt ctggtaccgg tgcacgtagc gaggacagtt cgggaagatc tcccgcgcgc
     1681 gcacgcggac cacgaactgc gcttccggcc actgcgagcg gagtacgtcg tcgaagtcca
     1741 cggaggcctc gccgttcacg cgcatccgat tccgccgctc gaagtcgatg aacaggatcc
     1801 ccacgtgcgg gttccggagc aggttccccg ccgacaggta catcccgttg ccgttgtagt
     1861 tcgggaacgc gagggtgtgc tcgtcgacga cacggatgaa gccggggtcg ccgcccttgt
     1921 agctcacgtt cggcttgccg tcctcgtcgg ccgtcgcgag gaagaacatg tcccgcgtct
     1981 cgacgaacgc gcggtcatgc tccgtgaacg tgtcgtggac cagcacgtct tcgatccggt
     2041 cggcgagccg gcgcgtgtcg aagcgatcct gcagctcgcg gttgccgtcg tggtacatct
     2101 cgtccatctc tgccgcccct cagaccagcg cgaggtcgaa tcgctcgccg tcccggtacg
     2161 tcgtccagat tctgtcgatc cgtacgtgga tgccgctctt gccgaggacc tcccaggccg
     2221 agccgtcgcc caccatcgcg gtgtcctcgt ccatcccgat gaaggtggaa ccctccgggg
     2281 cggactccac gaggaactcg gctgctcccg gcatccatgt gtccaccatg tcccagtgcg
     2341 gtgcgaagag cgtgcgcggg atcaggttca acccctgctt gaagatctga tcgacgtcgg
     2401 tcgtgtcgct gtcgaacgtg acctcgttga ggcacgcgac gccggcgctg cagcccgcgt
     2461 aggccatccc ccggtcgagc tgcttgacga gcgcgtccag gaagtcgctc tcggtgagcg
     2521 tcgatgcgag atacgcgggg ttcccaccgg agagatagac ggcggacgcg ccctcgagcc
     2581 gttcgatcag ccgcgggtcg tcggtgtcct ccctcgtctt gagctctagt acctcggcgg
     2641 cgatctccag tcgcgagaag tgggcgaggc cgagggatcc ccaacgctcg aacacgtcct
     2701 ttccctcggg ggcggacgcg gtggggagga tcagcacccg gccgtcaccg tccgcctcct
     2761 cgaccagtcg gcggtcgacc ggctccgacc agggttggaa ctcccccgag ccgagcagcg
     2821 accaccaggt catcgcaacg cctcgggacg gagctcgacg tcgagcttcg cgcccagctc
     2881 ggatgccaca tccaagagcg cgtccatgtc ctcggcctcc gtccggtagt tcacgatgca
     2941 cgaccgcagg cagaagcggt cgcccaggac ggcgttcgag cagtagacgc gcccgtccag
     3001 ctggatctcg gtcatcagcc ggttgttcag cagatcgagg tacgcgtctc ggtccggctc
     3061 gtttcctccg gcgcccgatg ccgagaggtc tgccggcacg tagcggaagc acgtgatcga
     3121 caggcccacc ggcgtcatca gctcgaactc gtccgactcc tccgcccgcg cgccgaggta
     3181 acgagcgagg gcggcatcgt gggatatccg acgcccgtag gccgtccgac cgtgcgcgag
     3241 gagtgacacc cacaccttca gcgcccagaa cccccgactg aagttgggcg agttccgtcc
     3301 gaggtcgatg ccccagtcag tgtgctcctc gtccttgacc acgtacgcga cgtagtcgac
     3361 gtcgaacgcc cgccgcatca actccatatc ccggacgatc acgcagccgc ccgattgcgg
     3421 tgtgtacagc cacttgtgcg ggtcgaacgc ca
//
LOCUS       DASUVE010000319         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1097397, whole genome shotgun sequence.
ACCESSION   DASUVE010000319 DASUVE010000000
VERSION     DASUVE010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097397"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VF036_06895"
     CDS             <1..312
                     /locus_tag="VF036_06895"
                     /inference="COORDINATES: protein motif:HMM:NF021941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX6264990.1"
                     /translation="TLAGGSRIELVTSADATRSARLIVQGETVLLPVGDLFDLDAERA
                     RLRKRLGDAEDERGRAAKKLANPAFRDKAPEDVVRAEEQKVERFEREAASLNERLAEL
                     G"
     gene            312..1601
                     /locus_tag="VF036_06900"
     CDS             312..1601
                     /locus_tag="VF036_06900"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX6264991.1"
                     /translation="MRFNEAVERLERRQPEHMPKPSLDRIRAVSALLDDPQRTYPTIH
                     VTGTNGKTTIARAAAELACAHGLTTGLFTSPHLHSVRERLSLCGEQISEEEFAEEWRH
                     LEPYLDVVDAQGNGAVTYFEAVTALAFLWFADKPVGLAVVEVGMGGSWDATNLVTGDV
                     AVIGEVGLDHPELGSTIREVATEKSGIVKTGRTVVVREQPDEALEVIQARANEVGATF
                     LLEGRDWELATRVPAVGGQQFRVDGVHAAYDDLFIPMFGEHAVLNVAAAMVAVESILG
                     HALDPDVTRGAIEALRIPGRLEVMSRSPLVVVDGAHNPGGAAALVRTLAEAFTWNRMY
                     VILAVSSNKDLDGVVAALAPIADVWFAARNDSVRSFPPEHVAERIATAGGRVADLGSV
                     EEGLAWGLDAAVPDDLILVTGSLYTVADARRALGGSG"
     gene            1604..2035
                     /gene="ndk"
                     /locus_tag="VF036_06905"
     CDS             1604..2035
                     /gene="ndk"
                     /locus_tag="VF036_06905"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX6264992.1"
                     /translation="MAPTDAPRDGKETTLLIVKPDGVRRHLIGEVLRRVEEAGLTIQD
                     LRMETIPRERAEEHYGEHRDKPFYRELVDFITGGPVVLAKVEGDNAIALWRELMGPTD
                     PADAPAGTIRGDLATEITENLVHGSDSPESAARELKLFFGD"
     gene            2043..>2439
                     /locus_tag="VF036_06910"
     CDS             2043..>2439
                     /locus_tag="VF036_06910"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6264993.1"
                     /translation="MAGLQEAFDRAGASLEHHLTASHAAGAALAVTDREEILGVAVRG
                     MADVASATPIRPETRFQIGSISKSFATIVAMQEVDAGRLDLHVSVNELLPWLALPEPF
                     GPITMHHLLTHTAGLHTGTEDAPGFAGALH"
BASE COUNT          374 a          830 c          903 g          332 t
ORIGIN      
        1 acgctcgcgg gcggctcgcg gatcgagctc gtgacctcgg cggacgctac ccggtccgcc
       61 cggctcatcg tgcagggcga gacggtcctg ctgcccgtcg gcgatctgtt cgacctggac
      121 gcggagcgtg cccggctgcg gaagcgtctc ggggacgccg aggatgagcg cggccgcgcc
      181 gcgaagaagc tcgcgaaccc cgcgttccgg gacaaggcgc ccgaggatgt cgtccgcgcc
      241 gaggagcaga aggtggaacg cttcgagcgg gaggcggcct cgctcaacga acggctcgcc
      301 gagctcgggt gatgcggttc aacgaagcgg tcgaacggct ggagcggcgc cagccggagc
      361 acatgccgaa gcccagcctc gacaggatcc gcgcggtgtc ggcgctcctg gacgatccgc
      421 agcgcacgta cccgacgatc cacgtcaccg gcacgaacgg gaagaccacg atcgcgcggg
      481 cggccgccga gctggcgtgc gcgcacgggc tcacgaccgg cctcttcacc tcgccacacc
      541 tgcactcggt cagggagcgt ctgagcctct gcggggagca gatctccgag gaggagttcg
      601 ccgaggagtg gcgccacctc gagccgtacc tcgacgtggt cgacgctcag gggaacgggg
      661 cggtcaccta cttcgaggcc gtcacggcgc tcgcgttcct ctggttcgcg gacaagccgg
      721 tgggtctggc ggtcgtcgag gtcgggatgg gcggctcgtg ggacgcgacg aacctcgtga
      781 cgggcgacgt cgcggtgatc ggcgaggtcg gcctcgatca cccggagctc ggctcgacga
      841 tccgagaggt cgccacggag aagtcgggga tcgtcaagac cggcaggacg gtcgtcgtgc
      901 gggagcagcc ggacgaggcg ctcgaggtga tccaggcgcg tgcgaacgag gtaggagcga
      961 cgttcctcct cgagggacgt gactgggagc tggccacccg ggtcccggcc gtcgggggtc
     1021 agcagttccg cgtcgacggc gtgcacgcgg cctacgacga tctgttcatc ccgatgttcg
     1081 gcgagcacgc ggtgctgaac gtcgccgccg cgatggtggc ggtcgagtcc atcctcgggc
     1141 acgcgctcga tccggacgtc acacgtggcg cgatcgaggc gctccggatc cccggccggc
     1201 tcgaggtgat gtcccgttcg ccgctggtcg tcgtggacgg cgcgcacaac ccgggcggcg
     1261 cggcggcgct cgtacggacg ctcgcggagg cgttcacctg gaaccggatg tacgtgatcc
     1321 tcgccgtctc gtcgaacaag gacctcgacg gcgtggtggc cgcgctcgcg ccgatcgccg
     1381 acgtgtggtt cgcggccagg aacgacagcg tgcggagctt cccccccgag cacgtcgcgg
     1441 aacgcatcgc cacggccgga ggccgggtcg ccgacctggg cagcgtggag gaagggctcg
     1501 cgtggggcct cgatgcggcc gtccccgacg acctcatcct cgtgacgggc tccctctaca
     1561 ctgtcgcgga cgcccgtcgg gcgctcggag gatccggctg acgatggccc caacggacgc
     1621 cccacgggac gggaaggaaa ccacgctgct gatcgtcaag ccggacggcg tccgtcgcca
     1681 tctgatcggc gaggtgctcc gccgggtcga ggaagccggg ctgacgatcc aggacctgcg
     1741 gatggagacg atcccgcggg agcgcgccga ggagcactac ggcgagcacc gcgacaagcc
     1801 gttctaccgg gagctcgtcg acttcatcac cggtggtccg gtggtcctgg ccaaggtcga
     1861 aggagacaac gcgatcgcgc tgtggcgcga gctgatgggc ccgacggacc cggcggacgc
     1921 acccgccggc acgatccgcg gcgacctcgc gacggagatc acggagaacc tcgtgcacgg
     1981 cagcgactct ccggagtcgg cggctcgcga gctcaagctc ttcttcggcg actagcgacg
     2041 ggatggcggg actccaggaa gcgttcgacc gcgccggcgc gagcctcgag caccatctca
     2101 ccgcctcaca cgccgccggg gcggcgctgg ccgtcacgga tcgcgaagag atcctgggcg
     2161 tcgccgtccg ggggatggcc gacgtcgcgt cggcgacgcc gatccgtccg gagacgcggt
     2221 tccagatcgg ctcgatctcg aagtcgttcg cgacgatcgt cgcgatgcag gaggtggacg
     2281 cggggaggct ggacctgcac gtctcggtga acgagctgct cccgtggctc gcgctccccg
     2341 agccgttcgg tccgatcacg atgcatcacc tgctcacgca cacggcgggc ctccacaccg
     2401 gaaccgagga cgccccggga ttcgcgggcg cgctccacc
//
LOCUS       DASUVE010000320         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_344471, whole genome shotgun sequence.
ACCESSION   DASUVE010000320 DASUVE010000000
VERSION     DASUVE010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344471"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VF036_06915"
     CDS             complement(<1..822)
                     /locus_tag="VF036_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX6264994.1"
                     /translation="MGFLGRDMAVDLGTANTLVYVRGRGVVLNEPSVVAINTLTGAIL
                     AVGAEAKRMIGRTPSHIQAVRPLKDGVIADFDVTEKMLRYFIQRVHKRRLLAKPRVVV
                     CVPSGITGVEQRAVEEATIAAGARRAYIIEEPMAAAIGAGLPIHEPTGNMVVDIGGGT
                     TEVAVISLGGVVTSTSIRIGGDELDESIIQHVKKEYSLLLGERTSEAIKLAIASVYPM
                     PEELVAEIKGRDLISGLPKTITIAAEEIRRAIEEPVNAIIDAIKTTLDRTPPELAA"
     gene            963..1868
                     /locus_tag="VF036_06920"
     CDS             963..1868
                     /locus_tag="VF036_06920"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX6264995.1"
                     /translation="MVFPGQGSQYAGMADPWVGHPAGRSILQEASDAMGRDIVAGCHD
                     DDALDTTEFVQPALLACDVAAFHVLEAEGVDPVGVAGHSLGQFAALVAAGVVSLADAL
                     DLVVTRGAAMQRAGEERPGAMTALLGVDAGDAASLCDEVRGEDELAVANENSPAQAVI
                     SGSVGAIERAETLAKERRIRAVRLPVAGAFHSLLMEPARAAIDERIDAIGFSAPRYPV
                     AENVAGTLVYDADELRSLLKRHVVSPVRWASSILALGGAGAGAFLEAGPGDVLTKLMK
                     RILPDAFALAVGSPEEARAAVLGAP"
     gene            1909..>2066
                     /locus_tag="VF036_06925"
     CDS             1909..>2066
                     /locus_tag="VF036_06925"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011569280.1"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HEX6264996.1"
                     /translation="MTRSATITGVGSTLPPRLVPNTWFEDKIDTNDEWIRERTGIEAR
                     HFVEEGVDT"
BASE COUNT          298 a          697 c          736 g          335 t
ORIGIN      
        1 cgcggcgagc tcaggggggg tccggtcgag ggtggtcttg atggcatcga tgatggcgtt
       61 caccggctcc tcgatcgccc ggcggatctc ctccgcagcg atcgtgatgg tcttcgggag
      121 gccggagatg aggtcgcggc ccttgatctc ggcgacgagt tcctccggca tcggatagac
      181 cgacgcgatc gccagcttga tcgcctcgga cgtccgttcg ccgaggagga gcgagtactc
      241 cttcttcacg tgttggatga tcgactcgtc gagctcgtcg ccgccgatgc ggatcgaggt
      301 cgacgtcacc acaccgccga gtgagatcac cgcgacctcg gtggtaccgc ccccgatgtc
      361 gacgaccatg ttcccggtcg gctcgtggat cgggagacct gcaccgatcg ctgccgccat
      421 cggctcctcg atgatgtacg cgcggcgcgc gccggcggcg atcgtggctt cctcgacggc
      481 gcgttgctcg accccggtga tgccggacgg cacgcacacg accacacgtg gcttcgcgag
      541 gagccggcgc ttgtgcaccc gctggatgaa gtagcggagc atcttctcgg tcacgtcgaa
      601 gtcggcgatc accccgtcct tcagtgggcg aacggcctgg atgtgcgagg gagttcggcc
      661 gatcatgcgc ttggcctcgg cacccacggc caggatggct ccagtaaggg tgttgatcgc
      721 aacgacggac ggctcgttca ggacgactcc gcgtccacgc acgtagacga gcgtgttcgc
      781 cgtcccgagg tcgacggcca tgtcgcgacc aaggaacccc aataccccct ccgacccctg
      841 tgctcggggc gtgggggaag aagatctctg aaccctctcg gccatgggct cgccctgatt
      901 gaacagggtt cgcctcctcg tggcaacctg ggggcgtggg tacgagcgga acgcgaatcg
      961 cggtggtctt cccgggccag ggatcccagt atgcggggat ggccgatccg tgggtcggcc
     1021 atccggccgg gagatcgatc ctgcaggagg cgtccgacgc gatgggtcgc gacatcgtgg
     1081 ccggctgtca cgacgacgat gccctcgaca ccacggagtt cgtgcagccc gcgctgctcg
     1141 cctgcgacgt cgcggcgttc cacgtgctcg aagcggaagg cgtcgacccg gtcggcgtgg
     1201 ccggtcactc cctcggccag ttcgcggcgc tcgtcgcggc cggcgtcgtc tcccttgccg
     1261 acgccctcga tctggtggtg acccggggcg ccgcgatgca gcgtgccggc gaggagcgtc
     1321 ccggagcgat gacggcgctg ctcggcgtcg acgccgggga cgccgcgtcg ctctgcgacg
     1381 aggtccgcgg cgaggacgag ctcgcggtgg cgaacgagaa ctccccggcg caggcggtga
     1441 tctccggatc ggtcggggcg atcgagcgag ccgagacgct cgccaaggag cggaggatcc
     1501 gcgcggttcg actcccggtc gccggtgcgt tccattcgct gttgatggag ccggcgcgcg
     1561 cggcgatcga tgagcgcatc gacgcgatcg ggttctccgc gccccggtac ccggtcgcgg
     1621 agaacgtggc gggtaccctc gtgtacgacg cggacgagct ccgctccctc ctgaagcgac
     1681 acgtcgtgtc accggtgcga tgggcgtcct cgatcctcgc cctcggcggg gccggggccg
     1741 gcgcgttcct cgaagcgggt cccggtgacg tgctcacgaa gctgatgaag cggatcctgc
     1801 cagatgcgtt cgccctggcg gtcggctcgc ccgaggaggc acgggccgcc gtgctgggcg
     1861 cgccctaggc gacccgactg tgggccgccg accgactaca ctcgccgcgt gaccaggagc
     1921 gccaccatca ccggcgtcgg gtccacgctc cctccgcgtc tcgttcccaa cacgtggttc
     1981 gaggacaaga tcgacaccaa cgacgagtgg atccgggagc ggaccgggat cgaggcccgc
     2041 cacttcgtcg aggagggcgt cgacac
//
LOCUS       DASUVE010000321         6113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1474918, whole genome shotgun sequence.
ACCESSION   DASUVE010000321 DASUVE010000000
VERSION     DASUVE010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474918"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VF036_06930"
     CDS             complement(<1..432)
                     /locus_tag="VF036_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6264997.1"
                     /translation="MGWIAAIDWEGGLENAWDAIIVFVPKLLGFLLILGIGYFVAKAI
                     EKVLDKLLERVGFDRIVERGGVQRALSRSRLDASSILSRIVFYIAFLFVLQLAFSVFD
                     PNPITDLLEGVIAFLPNLFVAVIIVVITAAIATGAKTIIESM"
     gene            complement(561..1916)
                     /locus_tag="VF036_06935"
     CDS             complement(561..1916)
                     /locus_tag="VF036_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX6264998.1"
                     /translation="MSDLGRTLLADEPVASIDAYLERGGGRGLAAAWALAPDEVIERV
                     RASGLRGRGGAGFPTGIKWRTVREDPCPTKYVVCNAAEGEPGTFKDRWLIRANPYLVV
                     EGLGIAAYAVGAERAFIGVKAGFGPEVARLTAAMEEMAEHDLVGPVPMELIQGPDEYL
                     FGEEKALLEVIEGNEPLPRIVPPWMEGLFRRPGSPNPTVANNLETLANVPLILDRGPD
                     WFRSLGTEDSPGTMLFTLSGDIRSPGVYELPLGYSLRDLIEGVGGGAPEGRRLKAVFP
                     GASNTIIAPEQLDTPLGFDTMGAVGTGLGSGGFVVYDDSACIVRATLAFSRFLYVESC
                     GQCPACKHGTGAITDLLERIDRGEGSEADVETILARALTVTDAQRCALPTGETLIAQS
                     AIQVFGSEFAEHFGRPCPRPRDLPVPKIVDFDERAGKFHLDERYHLKQPDWTYADETS
                     A"
     gene            complement(1945..2436)
                     /locus_tag="VF036_06940"
     CDS             complement(1945..2436)
                     /locus_tag="VF036_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HEX6264999.1"
                     /translation="MKAEDVVTYDYPAESYTEVVQGLTQASAAHSPAEMLSMFDVLID
                     VGGRDRWEGDADPRYSFHPLDDVPFIDDVEMIHAVGERIASLVREGKHVAVNCLSGVN
                     RSGLLVGRALVELGYTPKEAIEAVRSARGPMALSNQHFVRFLLVDCTPRALARRRQLS
                     LPF"
     gene            complement(2501..3577)
                     /locus_tag="VF036_06945"
     CDS             complement(2501..3577)
                     /locus_tag="VF036_06945"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6265000.1"
                     /translation="MSAIGSTLRRHWVEVAWGAFAAANVAVILLLSRWETIPFHFVWV
                     SLTLVYGFRGWRLRTTMALMLVVIAVTGAALAVSIVRGHEGPDELAEVPLMAAMFVAM
                     VWHAERRRAVSEQASRLAESEHQLLMAQREFVRDASHELRTPITVARGHAELIRDARA
                     GGQITQDAEVILDELEHLSRLSERLLILTTAGHPGFLSREPVDVSTFVEALARRWMPA
                     AARDWRVRLEARGTVQADPERLETALDGLLENAINATEDDEVIRVECRQAGDRIVIEV
                     ADSGTGIPCDDVPRIFDRFVRVDADRGRRNGGSGLGLPITKAIVEAHGGTIEVQSEEG
                     RGTTFLVTLEGFRPAPVPVPTGSG"
     gene            complement(3549..4214)
                     /locus_tag="VF036_06950"
     CDS             complement(3549..4214)
                     /locus_tag="VF036_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6265001.1"
                     /translation="MARVLVVDDEPRIVDFVSRALAAEGFVVDREYDGARALELAARE
                     RYELVVLDLLLPGLDGISVLHGLIEQRPDQRVVVLSALSDVETKVRCLESGASDYLSK
                     PFSLAELVARIRARLRQPAAGPRHRSLEAGGLRLDLTRRLAERDGTRVALSEREFLLL
                     EHLMRADGAVCSRQELLAEVWGYSFDPGSNVVEVCVRRLRAKVGADVIETVRNVGYRF
                     DAP"
     gene            4399..4842
                     /locus_tag="VF036_06955"
     CDS             4399..4842
                     /locus_tag="VF036_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265002.1"
                     /translation="MTARLHHAIAVPAGLVLAFAAASCGGGGGTVDVTLQEFSVATDP
                     TSAEAGEMTFEATNDGPDDTHEFVVFQTDLSLTDLPTDENGAVDEAGEGLELIGEIED
                     IEVGETDSVTLNLDAGNYVLVCNIWDEDEQEAHYQEGMRTGFTVS"
     gene            complement(4829..>6113)
                     /locus_tag="VF036_06960"
     CDS             complement(4829..>6113)
                     /locus_tag="VF036_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6265003.1"
                     /translation="LGPLAEAGRELGLRAARHYAASARRASDRSDDRAAATLFRHVAD
                     LLPEGHPDRPRALYDVGRASLRGLEPPVAFAALDGAVAAAAASGQRSIEWMARIDRAT
                     VQTLIDPIGFTTDDLRAELAAARAELESGDDEEALTVLWFGLVQVEWMPCRFDAAREA
                     AIRAVEHARRTGDRSLVMDATTLRLAAELLGSTSPAEGWPSIEEAVTEFGRDGLIGHV
                     VTVHEACLHAMTGDFARAREGIREAKALAERFGSQLWASAIYEFGGHIEEMAGDQEAA
                     ERSFRDEYELLGRMGDEGHGSTSAAYLAVVLCRLGRFDEAEELATIAHTTGADDDLAT
                     QASVCSAQAWVRSARGEHEEARRLAQEAVDLYAGAQSPWFHGDVLMVLSEVSRAAGLR
                     EEAAEAARAAMAAYERKGHEPGMASARASIGISSR"
BASE COUNT          990 a         2251 c         1981 g          891 t
ORIGIN      
        1 catcgactcg atgatcgtct tcgctcccgt ggcgatggcc gcggtgatca ccacgatgat
       61 gaccgccacg aacaggttcg ggaggaacgc gatgacccct tcgaggagat ccgtgatcgg
      121 gttcggatcg aacacgctga acgcgagctg gaggacgaac aggaacgcga tgtagaagac
      181 gatcctcgaa aggatcgagc tcgcgtccaa gcgcgaacgt gagagcgcgc gctgcacacc
      241 gccccgttcg acgatgcgat cgaatcccac tcgctccagg agcttgtcca agaccttctc
      301 gatcgccttc gcgacgaagt acccgatacc caatatcagg aggaacccca ggagcttggg
      361 aacgaacacg atgatcgcgt cccacgcgtt ctccaggcct ccctcccaat cgatcgctgc
      421 tatccagccc atgttcgtct ccttccggat cgaagtcgct cgtgccagtt gggctctcga
      481 taccccgagg tcgcggagag caatccggat cggcagggaa gactgctccc cgctgcccgg
      541 gcccgcgaac cgcggcccga ctacgcgctc gtctcgtccg cgtaggtcca gtcgggctgc
      601 ttcaggtggt accgctcgtc gaggtggaac ttccctgccc tctcgtcgaa gtcgacgatc
      661 ttcggtacgg gcaggtcccg ggggcgggga cacggccggc cgaagtgctc ggcgaactcg
      721 gacccgaaga cctggatggc gctctgcgcg atcagggtct cccccgtcgg gagcgcgcag
      781 cgctgggcat cggtgacggt cagagcccgt gccaggatcg tctccacgtc ggcttccgaa
      841 ccctcgcccc gatcgatccg ctcgagcaga tccgtgatcg ccccggtccc gtgcttacac
      901 gccgggcact gcccgcagga ctcgacgtac aggaaccgcg agaacgcgag cgtggcgcgc
      961 acgatacacg ccgagtcgtc gtagacgacg aacccgcccg agccgagccc ggtacccacc
     1021 gcacccatcg tgtcgaagcc gagcggcgtg tcgagctgct ccggggcgat gatcgtgttc
     1081 gatgctcccg ggaacaccgc cttcagacgg cgtccctccg gggcgccacc gcccacgccc
     1141 tcgatgaggt cacggagcga atatccgagg ggcagttcgt agacgcccgg tgagcggatg
     1201 tctccggaga gggtgaatag catcgtcccg ggcgaatcct ccgtgccgag cgaacggaac
     1261 cagtccggac cgcggtcgag gatcaggggg acgttcgcca gggtctcgag gttgttggcc
     1321 acggtgggat tcggcgaacc gggtcggcgg aacagacctt ccatccacgg cggcacgatc
     1381 ctggggagag gctcgttccc ttcgatcacc tcgagcaacg ccttctcctc gccgaacagg
     1441 tattcgtccg ggccctggat cagctccatg gggacgggac cgacgaggtc gtgctcggcc
     1501 atctcctcca tcgcggcggt gagccgagcc acctccgggc cgaaccccgc cttgacaccg
     1561 atgaacgccc gctcggcgcc gaccgcgtag gcggcgatcc cgagaccctc cacgaccagg
     1621 tacgggttcg cccggatcag ccaccggtcc ttgaacgtcc ccggctctcc ttcggcggcg
     1681 ttgcagacga cgtacttcgt cggacacggg tcctcccgga ccgtccgcca cttgatcccg
     1741 gtggggaacc ccgcgccgcc acgcccgcgc agccccgacg cgcggacccg ctcgatcacc
     1801 tcatcgggag ccagcgccca cgcggccgcg agcccccgcc cgccgccgcg ttcgaggtac
     1861 gcatcgatgg acgctacggg ctcgtccgcc aggagggtcc gtccgaggtc gctcacgtcg
     1921 atgccctccc ctcgaagcac gagatcagaa tgggagtgac agctggcggc gtcgtgccag
     1981 ggcgcgcggg gtgcagtcga cgaggaggaa gcgaacgaag tgctggttgc tgagcgccat
     2041 cggccctcgc gcgctgcgaa cggcctcgat cgcctccttg ggcgtgtagc cgagctccac
     2101 gagggcgcgc ccgacgagca gtccggacct gttcacgccg ctcagacagt tgaccgcgac
     2161 gtgcttcccc tccctgacga gggaggcgat ccgctcgccc acggcatgga tcatctcgac
     2221 atcgtcgatg aacgggacgt cgtcgagggg gtggaagctg taccgaggat cggcatcacc
     2281 ctcccaccga tcgcgacccc ccacgtcgat cagcacatcg aacatcgaga gcatctcggc
     2341 cggcgagtgc gcggcacttg cctgcgtgag tccctggaca acttccgtgt acgactccgc
     2401 cgggtagtcg tacgtgacga cgtcctccgc cttcatacct gcatggtcgg gtccgaagcc
     2461 cgtgttcgtc aatggattgc cgagcccggg gggcttccga tcaacccgag ccggtcggca
     2521 ccggaacggg agcgggtcgg aacccctcca gcgtgacgag gaaggtcgtg ccgcggccct
     2581 cctcgctctg cacctcgatc gttccgccgt gcgcctcgac gatcgccttc gtgatcggga
     2641 gcccgaggcc gctgccaccg ttacgccgac cgcgatcggc gtccacgcgc acgaagcgat
     2701 cgaagatccg ggggacgtcg tcgcacggga tgcccgtgcc cgagtcggcg acctcgatca
     2761 cgatgcgatc gccggcctgc cggcactcga cccggatcac ctcgtcgtcc tcggtcgcgt
     2821 tgatcgcgtt ctcgagcaac ccgtccagcg ccgtctcgag ccgctcgggg tcggcctgga
     2881 cggtgccccg cgcttccaag cgcacccgcc agtcgcgagc cgccgccggc atccaccgcc
     2941 gagccagcgc ctcgacgaac gtcgaaacgt ccaccggctc cctcgagagg aagccgggat
     3001 gaccggccgt ggtcaggatc agcaacctct ccgagagcct cgacagatgc tcgagctcgt
     3061 ccaggatcac ctcggcgtcc tgcgtgatct gtccgccagc ccgcgcgtcg cggatcagct
     3121 ccgcgtgccc gcgcgcgacc gtgatgggcg tccgcagctc gtgcgaggcg tcccgcacga
     3181 actcccgctg ggccatcagg agctgatgtt ccgactccgc cagccggctg gcctgttcgg
     3241 acaccgccct ccgacgctcg gcgtgccaaa ccatcgcgac gaacatcgcc gccatcaacg
     3301 gcacctcggc cagctcgtcg ggtccctcgt gcccgcggac gatcgacacg gcgagcgcgg
     3361 cgccggtcac ggcgatcacg acgagcatca gcgccatcgt ggttcgcagc cgccatccgc
     3421 ggaacccata gacgagcgtg aggctcaccc acacgaagtg gaacgggatc gtctcccaac
     3481 ggctgagcag caggatcact gcgacgttgg cggcggcgaa cgcgccccag gcgacctcga
     3541 cccagtgcct acggagcgtc gaaccgatag ccgacattgc gcaccgtctc gatcacgtcc
     3601 gccccgacct tggcccgaag gcgccggacg cagacctcca ccacgttgct gcccgggtcg
     3661 aaggagtagc cccagacctc ggcgagcaac tcctgcctgc tgcacacagc gccgtccgcg
     3721 cgcatcaggt gctcgaggag caggaactcc cgctcgctga gcgccacccg cgtcccgtct
     3781 ctctccgcca accgccgcgt gaggtccagg cgcagacccc ccgcctcgag ggatcgatgg
     3841 cgtgggccgg ctgccggctg acggagccgc gcccggatcc gcgcgacgag ctcggcgagc
     3901 gagaagggct tcgagaggta gtcggaggcg ccagactcca ggcaccggac cttcgtctcc
     3961 acgtccgaga gcgccgagag gacgaccacg cgctgatcgg gccgctgctc gatcaggccg
     4021 tggagcacag agatcccgtc gaggccgggg aggagcagat ccaggaccac cagctcgtag
     4081 cgttcccggg cggccagctc cagcgcgcga gctccgtcgt actcccggtc gaccacgaac
     4141 ccctcggccg cgagcgcccg ggagacgaag tcgacgatcc tcggttcgtc gtccaccacc
     4201 agcacccggg ccacgcgccg atgctatgcc cgctcgagcc gctccgcagc cccacatgac
     4261 cgagccgtca tgcgaacggg atgaccgtgc ggtgacggtt ccctgacgga gcggtcacgt
     4321 tccgccccgc ggcacgcgag ggcgcctacc gtcggtacca ccggccgcgc cgagccgcgg
     4381 cgtcgaaagg ggagagccgt gaccgcacgt ctgcaccatg cgatcgccgt tcccgccggc
     4441 ctcgtcctcg cgttcgctgc ggcgagctgc ggcggcggcg gggggaccgt cgacgtaacg
     4501 cttcaggagt tctcggtcgc taccgatccc acgtccgccg aagcgggtga gatgacgttc
     4561 gaggccacga acgacgggcc cgacgacacg cacgagttcg tggtcttcca gaccgacctc
     4621 agcctgaccg acctgccgac cgacgagaac ggtgcggtcg acgaggccgg cgagggcctc
     4681 gagctgatcg gtgagatcga ggacatcgag gtaggcgaga ccgacagcgt gaccctgaac
     4741 ctggacgccg gcaactacgt gctcgtctgc aacatctggg acgaggacga gcaggaggcg
     4801 cactatcagg agggcatgcg caccggtttc accgtgagct gatcccgatc gacgctcgcg
     4861 ccgacgccat ccccggctcg tggcccttgc gctcgtacgc ggccatggcg gcgcgcgccg
     4921 cttcggccgc ctcctcgcgc aggccggccg cccgtgagac ctccgagagc accatgagga
     4981 cgtccccatg gaaccaggga ctctgtgccc cggcgtagag gtccacggcc tcctgtgcga
     5041 gccggcgcgc ctcctcgtgc tcgccccgcg cggaccggac ccacgcctga gcggaacaga
     5101 cgctcgcctg cgtggccagg tcgtcgtcgg cgccggtggt gtgcgcgatc gtcgccagct
     5161 cctccgcctc gtcgaaccga cccagacggc acagcacgac ggcgaggtac gcggcggagg
     5221 tcgacccgtg cccctcgtcg cccatgcgcc cgaggagctc gtactcgtcg cggaacgatc
     5281 gttcggccgc ctcctgatcg cctgccatct cctcgatgtg accgccgaac tcgtagatcg
     5341 ccgatgccca caactgcgaa ccgaatctct cggccagcgc cttcgcctca cggatccctt
     5401 cacgggcgcg agcgaagtca ccggtcatcg catgcaaaca cgcttcgtgc accgtgacga
     5461 cgtgtccgat gagtccgtcg cgcccgaact cggtcaccgc ctcttcgatc gacggccacc
     5521 cttcggcggg cgacgtcgac ccgaggagct cggccgccag tctcagcgtc gtcgcgtcca
     5581 tcacgagcga tcgatctccg gtgcggcgcg cgtgctccac ggcgcggatc gccgcttccc
     5641 tcgccgcgtc gaagcggcac ggcatccact ccacctgcac gagcccgaac cacaggacgg
     5701 tgagcgcctc ctcatcgtcg cccgattcga gctcagccct cgccgccgcc agctccgctc
     5761 ggaggtcgtc ggtcgtgaac ccgatcgggt cgatcagcgt ctggacggtg gcccgatcga
     5821 tcctcgccat ccactcgatc gatcgctgcc cgctggcagc agcggcggcc acggctccgt
     5881 cgagggcagc gaacgccacg ggcggttcga ggccgcgcag gctcgcccgc ccgacgtcgt
     5941 acagagcgcg cggtcgatcc ggatgaccct ctgggaggag atcggccacg tgccggaaca
     6001 gggtcgcggc ggctcggtcg tcgctccgat ccgacgcccg gcgggcgctc gccgcgtagt
     6061 gccgggcagc cctgagcccc agctctcgtc cggcctcggc cagcggtccg agc
//
LOCUS       DASUVE010000322         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_534773, whole genome shotgun sequence.
ACCESSION   DASUVE010000322 DASUVE010000000
VERSION     DASUVE010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534773"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VF036_06965"
     CDS             complement(<1..902)
                     /locus_tag="VF036_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6265004.1"
                     /translation="MLAVDTFFSSQAMLAVVVALVALAAFALGWLLLGTAARVKADRD
                     MAARMAAIGRPVPQQTASAVGKPGSGWIPESVSSFGDRFAKAQGFGEKLDAQLEAAGV
                     SLRSGEFVVATVGAAILGGVLGAALLQNPLLAGVIGLLSAAAPTVLLRMSLGRRGDKM
                     REQLPDVLTIMASSLRAGHSFLQALDTVAREIPAPADVEFQRLVAEIRLGRPPEDALE
                     SLADRVGSADFRWAVLAVNIQREVGGNLAEILDNVADTLRERAMMRRQIKVLTAEGRL
                     SAWVLAILPFAIALYMFAVNPEYIS"
     gene            complement(905..>2008)
                     /locus_tag="VF036_06970"
     CDS             complement(905..>2008)
                     /locus_tag="VF036_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX6265005.1"
                     /translation="IVRQIGDSVLGLGPLEPYIRDPEVTEVMVNNWDTIYVERSGKLF
                     WTGTKFHDEGQLRRTIDKIVAKVGRRVDEASPMVDARLPDGSRVNAVIPPLAIDGPTL
                     TIRKFAADPYQADDLVEFGTMSSAVSKFLESCVRGRINVMVAGGTGAGKTTTLNVLSS
                     FIPDDERIVTIEDAAELKLQQPHVVRLEARPPNIEGKGQVSIRDLVRNSLRMRPDRIV
                     VGEVRGAEALDMLQAMNTGHDGSISTIHCNSPRDALSRLETITMMAGMDLGSRAIREQ
                     IASAIQLIVYQSRLKDGTRRFTHVTEVAGMEGEVITLQDIFLFDYSAGLDDEGRFRGR
                     LKSTGLRPKFVEKLQERGVHVDPELFALESRDR"
BASE COUNT          328 a          685 c          689 g          306 t
ORIGIN      
        1 gagatgtact cggggttcac ggcgaacatg tagagggcga tcgcgaacgg gaggatcgcc
       61 agaacccaag cggagagcct gccctcggcc gtgaggacct tgatctggcg gcgcatcatc
      121 gcgcgttccc ggagtgtgtc cgccacgttg tcgaggatct cggccaggtt cccgccgacc
      181 tcgcgctgga tgttcacggc gaggacggcc cagcggaagt ccgcgctccc gacgcggtcc
      241 gccagcgact cgagggcatc ctccggtgga cggcccagcc ggatctcggc cacgagccgc
      301 tggaactcca catcggccgg ggccgggatc tcccgggcaa cggtgtcgag cgcctggagg
      361 aacgagtgcc ccgcgcgcag cgacgacgcc atgatcgtca atacgtctgg cagctgctcg
      421 cgcatcttgt ccccccggcg acccagggac atccggagga gaacggtcgg cgcagcggcg
      481 ctgaggagac cgatcacacc cgccaggaga gggttctgga gcaacgccgc gccgagcacc
      541 ccgcccaaga tggcggcccc gacggtggcg accacgaact ctcccgaccg gaggctgaca
      601 ccggcggcct cgagctgggc gtcgagtttc tctccgaagc cctgcgcctt ggcgaaccga
      661 tcgccgaagg acgagacgct ctcggggatc cagccggatc ccggcttgcc gacggcggat
      721 gcggtctgct ggggcaccgg gcggccgatc gcagccatcc tcgccgccat gtcacggtcc
      781 gccttgacgc gagcggcggt ccccagcagg agccacccga gggcgaaggc cgccagcgcg
      841 acgagcgcga cgacgaccgc cagcatcgcc tggctcgaga agaaggtgtc gacagccagc
      901 atcgttatcg gtccctcgat tcgagcgcga acagctccgg gtcgacgtgc acgccgcgct
      961 cctggagctt ctcgacgaac ttgggccgca gacccgtgga cttcaagcgt ccccggaaac
     1021 gaccctcgtc atccagaccg gccgagtagt cgaacaggaa gatgtcctgg agcgtgatga
     1081 cctcgccctc catcccggcg acctcggtca cgtgcgtgaa ccggcgggtc ccgtccttga
     1141 ggcgcgactg gtagacgatc agctggatcg ccgatgcgat ctgctcgcgg atcgcgcgag
     1201 agccgaggtc catcccggcc atcatcgtga tggtctcgag acgggacagg gcgtcgcgcg
     1261 gcgagttgca gtggatcgtc gagatcgagc cgtcgtgacc ggtgttcatc gcctggagca
     1321 tgtccagcgc ctcggcgcca cggacctcac cgacgacgat ccggtcgggc cgcatccgca
     1381 acgagttccg gacgaggtcg cggatcgaga cctgcccctt gccctcgatg ttcggcggcc
     1441 gggcttcgag acggaccacg tgcggctgct ggagcttcag ctccgccgcg tcctcgatcg
     1501 tgacgatgcg ctcgtcgtcg gggatgaagg acgacagcac gttcagcgtg gtcgtcttcc
     1561 cggcgccggt tccgccggcc accatcacgt tgatgcggcc gcggacgcag gactcgagga
     1621 acttggagac cgccgagctc atcgtgccga actccaccag gtcgtccgcc tggtaggggt
     1681 cggcggcgaa cttccggatc gtgagcgtgg ggccgtcgat cgcgagcggc gggatgaccg
     1741 cgttcacacg cgaaccgtcg ggcaggcgcg cgtccaccat ggggctcgcc tcgtccacgc
     1801 gacgaccgac cttggcgacg atcttgtcga tcgtgcggcg gagctggcct tcgtcgtgga
     1861 acttcgtgcc cgtccagaac agcttgcccg atcgctcgac gtagatcgtg tcccagttgt
     1921 tcaccatgac ctcggtgacc tccgggtcgc ggatgtaggg ctcgagcggg ccgagaccga
     1981 ggacgctgtc accgatctgc cggacgat
//
LOCUS       DASUVE010000323         3931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1476476, whole genome shotgun sequence.
ACCESSION   DASUVE010000323 DASUVE010000000
VERSION     DASUVE010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476476"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VF036_06975"
     CDS             <1..324
                     /locus_tag="VF036_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX6265006.1"
                     /translation="AQGLMESQIRRLQEALRDPEIIEAPMAADAIMPHMLVTVRSLDD
                     EDDEETYLLAEHGEERAPGARTVTTSSPLGTALMGASKDQEVLVQAPGGSFRYVVVGF
                     EPFVG"
     gene            338..1012
                     /locus_tag="VF036_06980"
     CDS             338..1012
                     /locus_tag="VF036_06980"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX6265007.1"
                     /translation="MGRRAAAGHRPRPHVAREAPKVRDLPKPRKNESAAGGAIQWAFQ
                     WPYRAILAFLIWARLRPWHLTVAALVLNVATGVLIITGDWFLAGWALLAAGLCDVFDG
                     SVARHYGQQKRSGAFMDSVLDRVADAILFACLFWALAEDGRRLDAALALFTLVVSLSV
                     SHIRAEAEAVGVQLTEGFFQRLERVIALIIGLVVPGAMTPILLILAALGAVTALQRGW
                     SALVRA"
     gene            1017..1349
                     /locus_tag="VF036_06985"
     CDS             1017..1349
                     /locus_tag="VF036_06985"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6265008.1"
                     /translation="MDFVRVASLAEIPEGEMRAFDLSSGRVAVAHVEARLFAFADECT
                     HAGCALSEGDFDDRAATVECPCHGSVFDVENGEPVDGPAQDPVAIHLARLVDGWVELS
                     DQPVREGG"
     gene            1437..2840
                     /locus_tag="VF036_06990"
     CDS             1437..2840
                     /locus_tag="VF036_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX6265009.1"
                     /translation="MTDAPIRFDLPHDQLPTAWLNIMPSIAQAGIQPLPPLHPGTHEP
                     VTPDLFAPLFPEALVLQEVSTDPWIDIPGEILDVYRLWRPSPLQRAVRLEKALQTTAR
                     IYYKYEGVSPAGSHKPNTAIAQAFYNKEAGTKRLATETGAGQWGSALAMASKFFGIDC
                     QVFMVKASYEQKPYRRIFMETFGAEVVPSPSPTTQAGKAILEADPDSTGSLGIAISEA
                     VEVAATSGGAIKYSLGSVLNHVLLHQTVIGLEAKAQLEMTGDRPDVVIGCVGGGSNYA
                     GLAYPFMVDRLSGATPETRFLATEPAACPTLTKGRFAYDFGDTGETIPLVPMYTLGHT
                     FVPAPVHAGGLRYHGDAPSLSLLVRHGHMEAVAFSQNAVFDAAVQFAHTEGIVPAPES
                     AHAVRGAIDEAIAARDAGEERVILFGLSGHGAFDMQAYDDYMNGRLPEVEYRQEDVTA
                     SMANVPDVPIPGESTGP"
     gene            complement(2837..>3931)
                     /locus_tag="VF036_06995"
     CDS             complement(2837..>3931)
                     /locus_tag="VF036_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEX6265010.1"
                     /translation="EEGEDMSESRGPGEVASAAEAPELAAKGLYGRRLMPAGRVLVVL
                     LVSLLTWTLLYAPALKRAAEASPIGTRRTLSLAILTPIAAVSDWIGLDQLATTIERAV
                     GHDAGKSDGAFVPPPDDIPVAPPDDPEDESGDGGENEPIRIPTPERKLRVAIVGDSLA
                     GGLGYFAERVFRPRLVTVSGQGRISTGLARPDYFNWPFTMRRIVDRFDPDLVIVMLGE
                     NDHQSLQDVHGNREALIGTAGWPPAYRDRVLTMMRIATSRGGKVVWAGLPISADYGLR
                     EHSRRQNDLIEFAASISSDVVYFDTWNRFRDPAGGYTAYYREGSRVILIREGDGLHFN
                     AVGYTLVAREIAKLAAEEFGLSPRTFETEI"
BASE COUNT          594 a         1349 c         1370 g          618 t
ORIGIN      
        1 gcgcagggcc tgatggaatc gcagatccgc cggctccagg aggcgctccg cgatccggag
       61 atcatcgagg caccgatggc ggcggacgcg atcatgccgc acatgctcgt caccgtccgc
      121 tcgctggacg acgaggacga cgaggagacc tacctcctgg cggagcacgg cgaggagcga
      181 gctcctggtg cgcgaacggt cacgacgtcg tcccccctcg ggacggccct gatgggcgcg
      241 tcgaaggatc aggaggtgct cgtgcaggcg cccggcggga gcttccgcta cgtggtcgtc
      301 ggcttcgagc cgttcgtcgg ctgatgagcg acagctgatg ggccggcgcg cggccgcggg
      361 gcatcgcccg agaccccacg tcgcgcgcga ggcgccgaag gtccgcgacc tcccgaaacc
      421 tcggaagaac gaatcggcgg ccggcggcgc gatccagtgg gcgttccagt ggccgtaccg
      481 ggcgatcctc gcgttcctca tctgggcacg gctgcgaccg tggcacctga cggtcgcggc
      541 gctcgtcctc aacgtggcga cgggggtcct gatcatcacg ggggactggt tcctcgccgg
      601 ctgggccctg ctcgcggccg gcctgtgcga cgtcttcgac ggatcggtcg cgaggcacta
      661 cgggcagcag aagcgatcgg gagcgttcat ggactcggtc ctggaccgcg tcgccgacgc
      721 gatcctgttc gcgtgcctgt tctgggcgct cgcggaggat gggaggcgcc tcgacgccgc
      781 cctcgcactc ttcaccctgg tggtgagcct gtccgtgagc cacatccgag ccgaggccga
      841 ggccgtcggt gtgcagctca ccgaggggtt cttccaacga ctggagcgag tcatcgcgct
      901 gatcatcggg ctcgtcgttc cgggcgccat gaccccgatc ctgttgatcc tcgccgcgct
      961 cggagcggtg acggcgctgc agcgcggctg gtccgccctc gtgcgagcct aggccgatgg
     1021 acttcgtgcg cgtcgcttcg ctcgccgaga tccccgaggg cgagatgcgg gcgttcgatc
     1081 tgtcgtccgg acgggtggcc gtcgcgcacg tggaagcgag gctgttcgcg ttcgccgacg
     1141 agtgcaccca tgcggggtgc gctctctcgg agggtgactt cgacgatcgg gcggccactg
     1201 tcgagtgccc gtgccacggg agcgtcttcg acgtcgagaa cggagagccg gtcgatggcc
     1261 ccgctcaaga cccggtcgcg atccacctgg cgcggctcgt ggacggatgg gtcgagctct
     1321 ccgatcagcc ggttcgcgag ggtggctgac ccgggactgg ctcccggaac gcgctccggg
     1381 taggctcgcc cctcgtccgc tctgctggct tctggcgaaa cgaggaggct tccccgatga
     1441 ccgacgcgcc gatccgattc gatctccccc acgatcagct gccgaccgcg tggctgaaca
     1501 tcatgccctc catcgcgcag gccgggatcc agcccttgcc gccgctgcac ccggggacgc
     1561 acgagcccgt cacgccggat ctcttcgccc cgctgttccc ggaagcgctg gtcctccaag
     1621 aggtctccac cgatccgtgg atcgacatcc cgggcgagat cctcgacgtc taccggctct
     1681 ggcggccgtc cccgctgcag cgagccgtcc gtctcgagaa ggcgctccag accacagcgc
     1741 ggatctacta caagtacgaa ggcgtctccc cggcggggag ccacaagccg aacacggcga
     1801 tcgcgcaggc cttctacaac aaggaggcgg ggacgaagcg cctggccacg gagaccggcg
     1861 ccggtcagtg gggctccgcg ctcgcgatgg cgtcgaagtt cttcggcatc gactgccagg
     1921 tcttcatggt gaaggcctcg tacgagcaga agccgtaccg acggatcttc atggaaacgt
     1981 tcggcgccga ggtcgtgccg tcaccgtcac cgaccacgca ggcggggaag gcgatcctcg
     2041 aggccgaccc ggactcgacc ggctccctcg ggatcgcgat cagcgaggcc gtcgaggtcg
     2101 ccgccacgtc cggcggagcg atcaagtact cgctcggctc cgtgttgaac cacgtgctgt
     2161 tgcaccagac cgtgatcggt ctcgaggcga aggcgcagtt ggagatgacg ggcgatcggc
     2221 ccgacgtcgt gatcgggtgc gtgggcggcg gatcgaacta cgccggcctc gcgtacccgt
     2281 tcatggtcga tcggctctcg ggagcgacgc cggagacgcg gttcctggcc accgagccgg
     2341 cggcgtgtcc cacgctcacg aaaggccggt tcgcctacga cttcggcgac acgggcgaga
     2401 cgatcccgct ggtgccgatg tacaccctcg gccacacgtt cgtcccggcc ccggtgcatg
     2461 cggggggact gcgctaccac ggagatgcac cgtcgctctc gctcctcgtc aggcacgggc
     2521 acatggaggc cgtcgccttc tcccagaacg cggtgttcga cgcggccgtg cagttcgcac
     2581 acacggaggg gatcgtgccg gcccccgagt cggctcacgc cgtccgggga gcgatcgatg
     2641 aggcgatcgc ggcgcgcgat gccggagagg agcgcgtgat cctcttcggg ctgtcgggtc
     2701 acggggcctt cgacatgcag gcctacgacg actacatgaa cggccgcctg cccgaggtgg
     2761 agtaccggca ggaagacgtg acggcatcga tggcgaacgt gcccgacgta ccgatccccg
     2821 gggagtccac cggaccctag atctccgtct cgaacgtccg cggcgacagg ccgaactcct
     2881 ccgcggcgag cttcgcgatc tctcgcgcga cgagcgtgta gccgacagcg ttgaagtgga
     2941 gaccgtcacc ttccctgatc aggatcacgc ggctgccttc gcggtagtac gcggtgtatc
     3001 cgccggccgg atcgcggaag cggttccacg tgtcgaagta gacgacatcg ctggagatgc
     3061 tcgcggcgaa ctcgatgagg tcgttctgac gtctcgagtg ctcgcgcaac ccgtagtcgg
     3121 cggagatggg caggccggcc cacacgacct tccccccgcg cgacgtggcg atgcgcatca
     3181 tcgtgagcac ccggtcccgg tacgcgggtg gccatcccgc ggtgccgatc agtgcctccc
     3241 ggttgccgtg cacgtcctgc aacgattgat ggtcgttctc gccgagcatc acgatcacga
     3301 ggtcgggatc gaatcggtcg acgatccgac gcatcgtgaa cggccagttg aagtagtccg
     3361 ggcgtgccag tcccgtcgag atccggcctt gccccgagac ggtcaccagc cgcggccgga
     3421 acacccgctc ggcgaagtag ccgaggccac cggcgaggga gtcgccgacg atcgccacgc
     3481 ggagctttcg ctccggcgtc gggatgcgga tgggttcgtt ctcgccacca tctcccgact
     3541 cgtcctccgg atcgtcaggc ggcgcgaccg ggatgtcgtc gggcgggggt acgaacgcgc
     3601 cgtccgactt gcccgcgtcg tgaccgacgg cgcgctcgat cgtcgtggcg agctggtcga
     3661 gaccgatcca atcgctcacc gcggcgatcg gcgtgaggat cgcgagcgaa agcgtgcgcc
     3721 gagttccgat cggcgaggcc tccgctgccc gtttcaaggc cggcgcgtac aggagcgtcc
     3781 acgtgaggag agagacgagc aggaccacga gcacccgacc cgcgggcatc agccggcggc
     3841 cgtagaggcc cttggcggcg agctccggag cctcggccgc cgacgcgacc tcgcctggtc
     3901 cccgcgactc gctcatgtcc tcgccctctt c
//
LOCUS       DASUVE010000324         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_724155, whole genome shotgun sequence.
ACCESSION   DASUVE010000324 DASUVE010000000
VERSION     DASUVE010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724155"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VF036_07000"
     CDS             <1..433
                     /locus_tag="VF036_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit gamma"
                     /protein_id="HEX6265011.1"
                     /translation="RRRDYGRISVLYGARTPADLLFRDEVEGWRGRLDVDVEVTVDAA
                     APDWRGNVGVVPGLLARARFDPADAVAMVCGPEVMIRFTVQALLDRGVAADRILVSLE
                     RNMQCAIKQCGHCQLGPYFVCADGPVMPYGLVAPWLRPREV"
     gene            433..1209
                     /locus_tag="VF036_07005"
     CDS             433..1209
                     /locus_tag="VF036_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX6265012.1"
                     /translation="MAARPRLAVWKFASCDGCQLTLLNCEDELLPVAGALEIASFPEA
                     SRAVIDGPYDLSLVEGSITTPSDLERIQEVRRQSRFLVTIGACATSGGIQALRNFKDV
                     NDFMRIVYAQPEYIETLETSTAIAEHVPVDFELRGCPIDRSQLLEVVGAFLNGRRPQV
                     PNHSVCIECKRRGTVCVMVAQGTACLGPVTHAGCGALCPSYDRGCYGCFGPMETPNTT
                     SLGHHLLQIGVREDDVVRVFRTFNANAEAFREESEARDAR"
     gene            1196..2509
                     /locus_tag="VF036_07010"
     CDS             1196..2509
                     /locus_tag="VF036_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEX6265013.1"
                     /translation="MTHGSSPSADAAVHVPGLARVEGEGGLEIRVRDGEVLEARLNIY
                     EPPRFFEAFLRGRRYTEPPDITARICGICPIAYQMSACRAIEDACGVTVVGPIATLRR
                     LIYLGEWIESHALHVYLLHAPDFLGYESAVHMAADHREVVEQGLAMKKAGNGLMEVIG
                     GRSVHPINVRVGGFYRAPTREELRALLQPLERAREIAVETARWVSGFDFPPFERDYGF
                     VSLRHPTDYPVTEGRIASSDGLDIAVAQYDEHFVEEHVPHSTALHSRLRSTGDHYLVG
                     PLARFNLNRDRLRPLARRAAEEAGIADGCRNPFRSIVVRAVELVHACDEAIDIIGSYE
                     PPDPPAVEVPPRAGAGTGATEAPRGMLYHRYVLDPDGVIEDARIVPPTSQNQGTIEED
                     LVRFAGDRLSLDRDALTWQCEQAVRNHDPCISCATHFLDLRVVEA"
BASE COUNT          390 a          899 c          909 g          399 t
ORIGIN      
        1 gaggcgccgg gactacggcc gcatctcggt gctctacggc gcccgaacgc ccgccgacct
       61 cctgttccgc gacgaggtcg aaggctggcg cgggcgtctc gacgtggacg tcgaggtcac
      121 ggtggacgcg gccgctccgg actggcgagg gaacgtcggc gtggtgcccg gactgctcgc
      181 gagggcgcgg ttcgacccgg ccgacgccgt tgcgatggtc tgcgggcccg aggtcatgat
      241 tcggttcacg gtccaggcgc ttctcgaccg aggcgtcgcc gccgaccgga tcctcgtgtc
      301 gctcgaacgg aacatgcagt gcgcgatcaa gcagtgcggc cactgccagc tcggtccgta
      361 cttcgtgtgc gcggacggcc ccgtgatgcc ctacgggctc gtcgcgccct ggcttcgccc
      421 acgggaggtg tgatggcggc gcgaccccgg ctcgccgtat ggaagttcgc ctcgtgcgac
      481 gggtgtcagc tgaccctcct gaactgcgag gacgagctgc tgcccgtcgc cggagcgctc
      541 gagatcgcct cgttccccga agcttcccgc gcggtgatcg acggcccgta cgacctctcg
      601 ctcgtggaag ggtcgatcac gaccccgtcc gaccttgaac gcatccaaga agtccgacgc
      661 cagtcccgat tcctcgtcac gatcggcgcg tgcgccacgt cgggcgggat ccaggcgctt
      721 cggaacttca aggacgtcaa cgacttcatg cggatcgtct acgcgcaacc cgagtacatc
      781 gagaccctcg agacctcgac ggcgatcgcg gaacacgtgc cggtcgactt cgagctgcga
      841 gggtgtccga tcgaccggag tcagctgctc gaggtggtcg gggcgttcct gaacggacgg
      901 cgcccccagg tcccgaacca cagcgtatgc atcgagtgca agcggcgcgg aaccgtctgc
      961 gtgatggtcg cccagggcac cgcgtgcctc ggcccggtca cgcacgccgg ctgtggcgcg
     1021 ctctgtccgt cctacgaccg aggctgctac ggatgcttcg gcccgatgga gacaccgaac
     1081 acgacgtcac tcggacacca tctcctgcag atcggggtcc gtgaggacga cgtcgtgcgg
     1141 gttttccgca cgttcaacgc caacgcggag gcgttccgcg aggagagcga ggcccgtgac
     1201 gcacggtagc tcgcccagcg ccgatgccgc cgtccatgtc cccggcctcg cccgtgtgga
     1261 gggcgagggc gggctcgaga tccgcgtgcg cgacggcgag gtgctggagg ctcggctgaa
     1321 catctacgag ccgccccggt tcttcgaggc gttcctcagg ggacgccgtt acaccgagcc
     1381 gccggacatc accgcgcgga tctgcgggat ctgcccgatc gcctaccaga tgagcgcctg
     1441 ccgggcgatc gaggacgcgt gcggggtcac ggtggtcggg cccatcgcga cgctccggcg
     1501 cctgatctac ctcggcgagt ggatcgagag ccacgccctg cacgtgtacc tgctgcacgc
     1561 tcccgacttc ctcgggtacg agagcgcggt gcatatggcc gccgaccacc gggaggtggt
     1621 cgagcagggc ctcgcgatga agaaggccgg caacggcctg atggaggtga tcggcgggcg
     1681 ctcggtccac ccgatcaacg tccgggtcgg cggcttctat cgcgccccga cccgcgagga
     1741 gctccgagcg ctactgcaac ctctcgaacg ggcccgcgag atcgccgtgg aaacagccag
     1801 atgggtctcg gggttcgact tccccccgtt cgaacgggac tacggtttcg tctcgttgcg
     1861 gcatccgacg gattacccgg tcaccgaggg ccggatcgcc tcgagcgacg gcctcgacat
     1921 cgcggtcgcg cagtacgacg agcacttcgt ggaggaacac gtgccgcact cgaccgcgct
     1981 gcactcgcgg ctgcggtcga ccggtgacca ctacctcgtg gggcccctcg cgcgcttcaa
     2041 cctgaacagg gatcgactcc gcccgctcgc ccgccgtgcg gccgaggaag ccgggatcgc
     2101 cgacggctgc cggaacccgt tccgcagcat cgtcgtgcgc gccgtcgagc tcgtgcacgc
     2161 gtgcgacgag gcgatcgaca tcatcgggtc gtacgagccg ccggatccgc ccgcggtcga
     2221 ggtgccgccg agggccggcg ccgggaccgg tgcgaccgag gcgccccgcg gcatgctcta
     2281 ccaccggtac gtgctcgacc ccgacggggt gatcgaggac gcgcggatcg tcccgccgac
     2341 ctcgcagaat caggggacga tcgaggagga tctggtccgc ttcgcgggtg accggctgtc
     2401 gctcgaccgc gatgcgctca cctggcagtg cgagcaggcg gtccggaacc acgacccctg
     2461 catctcgtgc gcgacgcact tcctcgatct ccgggtggtt gaggcatgat cgcgaacgac
     2521 cggggcatcg tcacggtgat cgggatcggg aaccggttcc ggcgggacga cgccgcgggt
     2581 ctcgaggtgg cgcggaa
//
LOCUS       DASUVE010000325         6633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_159539, whole genome shotgun sequence.
ACCESSION   DASUVE010000325 DASUVE010000000
VERSION     DASUVE010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159539"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VF036_07015"
     CDS             complement(<1..320)
                     /locus_tag="VF036_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265014.1"
                     /translation="MPDDERESDDLFEDLDKFFAPIKDVDWDEREGTPSTTASAAQEV
                     TEEEHVTVRSEPEQVVSVPAADDETPGTVFDDDDDDDDGDWYDTASLEPVGQALGGAV
                     GGDDA"
     gene            complement(320..880)
                     /gene="frr"
                     /locus_tag="VF036_07020"
     CDS             complement(320..880)
                     /gene="frr"
                     /locus_tag="VF036_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX6265015.1"
                     /translation="MTIDSSLKEADHKMEQALAHLREDLSSVRTGRASGSVLSRITVE
                     YYGAPVPLNQLASVNVPEPRLLVVQPFDKNSISQIEKAIQQSDLGITPSNDGQVIRLA
                     FPPLTEERRKDLVKQVHHRAEEGRVAVRNVRRHAKDDMEKLEREHSISEDELTRGEKE
                     LQKLTDRYVASIDEVQRHKESELMEV"
     gene            complement(877..1665)
                     /gene="pyrH"
                     /locus_tag="VF036_07025"
     CDS             complement(877..1665)
                     /gene="pyrH"
                     /locus_tag="VF036_07025"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX6265016.1"
                     /translation="MVLDADAAVTITRGAHANVTERSPGGTGYHRVLLKLSGEAFAPT
                     DGTGISTEATALIGRQLAEVVDLGTQVAVVVGGGNIWRGRQAPAIERSRADYMGMLAT
                     VMNSLALQDSLEKLHVPTRVQTAIQMTQLAEPFIPLRARRHLEKGRVVIFAAGLGVPY
                     FSTDTCAAQRALEIGANALLKGTKVDGVYTADPNLVPGASRLAEVGYVDILREGYQVM
                     DATAISLCMENDLPIIVFDLWEEGNIRRVVMGETVGSLVHGGTR"
     gene            complement(1665..2276)
                     /gene="tsf"
                     /locus_tag="VF036_07030"
     CDS             complement(1665..2276)
                     /gene="tsf"
                     /locus_tag="VF036_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX6265017.1"
                     /translation="MTDISADQVKTLRERTGAGMMDCKRALQETDGDLVKAIELLRER
                     GLASVAKRAGRSADQGLIDSYIHFNNSVGVLIEVNSETDFVANTDEFKQLVKDIALHI
                     ASPAAPRWLTREDVPADIVQQEEHIALVQAKEAGKPENVLDRIVEGKLNAFYQGSVLL
                     EQPYVKDDGKTIQQLVDEVAAKVGEKVAVRRFVRYKLGESTED"
     gene            complement(2273..3205)
                     /gene="rpsB"
                     /locus_tag="VF036_07035"
     CDS             complement(2273..3205)
                     /gene="rpsB"
                     /locus_tag="VF036_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX6265018.1"
                     /translation="MPVVTRRELLEAGVHFGHQTRRWNPKMQRYLFGERSGIYIIDLE
                     KSLAGIEETFEFVRDLGRRRGTVLFIGTKKQAQEVVEEHAGRVGMPFVNNRWLGGMLT
                     NFSTISKRLVRLRELREMESSDAMDNLPKKEVIRLRREREKLERNLGGIQALERLPDA
                     VFVVDTKKEQIAVTEARKLEIPVIAIVDTNCDPDEVDYVIPGNDDAIRAVSLVARVVA
                     DALAEGYGMAKDEVIEKSVARRPTAAPRRSTTAAPAPADEDVPSAEDAAAIAATTVFE
                     PDLPTPETAEGEPEVVAEPTMEESASAAPSKGEA"
     gene            complement(3373..4317)
                     /locus_tag="VF036_07040"
     CDS             complement(3373..4317)
                     /locus_tag="VF036_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265019.1"
                     /translation="MSADSRGTRGRPAPVRRGGLLSRMSVPPMDSMPPIRTAFARGLA
                     ATWSSPLVVGATVAWLLLEWLVVVALGYPGPFALLAHIVAPAPLSTTTDLSVSIGILG
                     VGKGLPLVLIPALVHALWHAIVVGLAVEAVETGRASRWGAIRGLRALPVAFAIHAFGV
                     AVLFTSQIVAGLGGGGLSFILQLSILVLAVWVFAFAPVIAVTEGRRFLDCLGRALRAA
                     RLPGSGNLTFAVLYVVPWFATSVATIVGSVPGSGLGVNPPYSAWIFVVVVSLLHTAII
                     GAIAIRYLAIAGEVPDAPVRAAPERRASRPRGVRTGRA"
     gene            complement(4314..5771)
                     /locus_tag="VF036_07045"
     CDS             complement(4314..5771)
                     /locus_tag="VF036_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX6265020.1"
                     /translation="MTLAGCTLAVVLGMPLVALAEWPQFQGGPSHDGLSDGPSAPLEV
                     AWRHRDIAIDDADVTGGLSSPIVGDDGTIVVVGPHEVFGFDAEDGSEVFTAPRDLGPS
                     VTPAIGSGPDGPVVVFAEGFGDRGPATTASPTASPNASPSAPPGEEGHTGSHLVAIDL
                     PSGETVWPSSVPLEDVVRTPVTADETAAYVGDYSGHVTAVELSSGELRWSKDLGSSIA
                     GAVTVDGDRALVATVGEQQTLGAVVALDRSTGEELWRTGEDAVQGNLVSGPVVADGMI
                     LVFEPGSVIALEAAEGRFLWRSEIVNPLTTPFSPQGISTLAPVMADGTVYAVDVTGRA
                     YGLDAETGTVLWDHAVNDPSPLTPPMLNHAHLLVPTNSGSLYAIDRRTGHLAFRIYAG
                     DAFLRGLADAGDVLVAVTGVEDAEIVAFAEDPDGVLTDEPSPTTFELGGLLAGFALGG
                     LPVAIVALALAWPLQRRLGPTPRPGSDLDPEEEAG"
     gene            complement(5849..6346)
                     /locus_tag="VF036_07050"
     CDS             complement(5849..6346)
                     /locus_tag="VF036_07050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6265021.1"
                     /translation="MRKLVLWCGVWSAVLVSAVPAPAHASGDWTWPIVAPVVRGFDPP
                     DSPFGSGHRGIDIAAPVGTPVRAATTGTVTFAGPVGGRLFVTVDHGAGLESTYSFLDS
                     VAVRRGDVVSPGQAIARSGTGHAGDIVPVLHFGVKLADTYVDPLEYLGPVEVWRFIRL
                     APLES"
     gene            complement(6408..>6633)
                     /locus_tag="VF036_07055"
     CDS             complement(6408..>6633)
                     /locus_tag="VF036_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008745397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6265022.1"
                     /translation="IDPTWGVESQETRGMLAAAINSLSEREKIVVTLYYFEGLTLAEI
                     GEILGVTESRVCQIHTKAVGGLRGQLSEFD"
BASE COUNT         1046 a         2333 c         2213 g         1041 t
ORIGIN      
        1 gcgtcgtcgc cgccgacggc gcctccgaga gcctgcccca ccggttcgag cgacgccgtg
       61 tcgtaccagt ctccgtcgtc atcatcatcg tcgtcatcga agacggtccc gggcgtctcg
      121 tcgtccgctg cgggcaccga gaccacctgc tcaggctcgc tccgcaccgt gacgtgctct
      181 tcctcggtta cctcttgcgc cgccgaggcc gtcgtggagg gagtcccctc ccgctcgtcc
      241 caatcgacgt ccttgatggg ggcgaagaac ttgtcgagat cctcgaacag atcgtcgctc
      301 tcgcgctcgt cgtcgggcac tagacctcca tgagctcaga ttccttgtgg cgctgcacct
      361 cgtcgatcga tgccacgtat cggtccgtca gcttctggag ctccttctct cccctcgtga
      421 gctcatcttc cgagatggag tgctcccgct caagcttctc catatcatcc ttcgcgtgcc
      481 ggcggacatt gcggaccgcg acacggccct cttcggctcg atgatggacc tgtttcacga
      541 ggtccttccg gcgctcctcc gtgagcggag ggaacgccag tcggatcacc tgcccgtcgt
      601 tcgacggcgt gatcccgagg tcgctctgct ggatcgcctt ctcgatctgt gagatcgagt
      661 tcttgtcgaa cggctggacg acgagcaggc ggggctcggg cacgttcacg gatgcgagct
      721 gattcagcgg aacgggcgct ccgtagtact cgacggtgat ccggctgagc acggatccgg
      781 aggcccggcc ggtccggacc gaggaaaggt cctcgcggag atgggcgagt gcctgttcca
      841 tcttgtggtc cgcctccttg agggacgaat cgatcgtcat ctcgtccctc catggaccag
      901 cgaacctacg gtctcaccca tcaccacgcg ccggatgttc ccctcttccc agagatcgaa
      961 gacgatgatc ggcaggtcgt tctccatgca gagcgagatc gccgtggcgt ccatcacctg
     1021 gtagccctcg cggaggatgt cgacgtagcc gacctccgcc aagcgcgaag cccccgggac
     1081 gaggttgggg tcggccgtgt acacgccgtc caccttcgtt cccttcagga gcgcgttggc
     1141 accgatctcg agcgctcgtt gcgcagcgca ggtatcggtg gagaagtacg gcacgcccag
     1201 accggctgcg aagatcacca cgcgcccctt ctcgaggtga cgtcgcgccc gcagcgggat
     1261 gaacggctcg gcgagctgcg tcatctggat cgcggtctgc acccgcgtgg gtacgtgcag
     1321 cttctcgagc gagtcctgca gcgccagcga gttcatcacc gtggcgagca tccccatgta
     1381 gtcggcccgg ctccgctcga tcgcgggcgc ctgccgcccc cgccagatgt tgcccccgcc
     1441 gaccaccacc gcgacctgtg tgccgaggtc gacgacctcg gcgagctgcc ggccgatcag
     1501 cgcggtcgcc tccgtgctga tccccgtgcc gtcggtgggt gcgaacgcct cgccggagag
     1561 cttgaggagg acgcggtggt atcccgtccc cccgggactc ctctccgtca cgttcgcgtg
     1621 cgctcccctc gtgatcgtca cggccgcatc ggcgtcgagg accatcagtc ctcggtggat
     1681 tcaccaagct tgtatcggac gaatcgacgg acggcaacct tctcgccgac cttggcggcg
     1741 acctcatcga ccaactgctg gatcgtcttc ccatcgtcct tcacgtacgg ctgctccaag
     1801 agcacggagc cttgatagaa ggcgttgagc ttgccctcga cgatccggtc gagcacgttc
     1861 tcgggcttcc cggcctcttt cgcctgcacg agggcgatgt gctcctcctg ctggacgata
     1921 tccgcgggga cgtcctcgcg ggtgagccag cggggagcgg cggggctcgc gatatggagc
     1981 gcgatgtctt tcacgagctg cttgaactcg tccgtgttcg ccacgaagtc cgtctcgctg
     2041 ttgacctcga tcagcacgcc caccgagttg ttgaaatgga tgtagctgtc gatcagaccc
     2101 tgatcggccg aacggccggc ccgcttggcg accgacgcga gtccccgctc ccgcagcagc
     2161 tcgatcgcct tcacgaggtc gccgtcggtc tcctggagcg cacgcttgca atccatcatc
     2221 cccgcgccgg tgcgctcgcg gagcgtcttc acctggtcgg cggagatgtc ggtcatgcct
     2281 cgcccttcga cggagcggca gacgccgatt cctccatcgt gggttcggcg accacctccg
     2341 gctcgccctc ggcggtctcc ggggtcggga ggtcgggctc gaacaccgtg gtggccgcga
     2401 tcgcggcagc gtcctcggcg gacggcacgt cctcgtccgc cggagccggg gcagcggtcg
     2461 tggaccggcg cggtgccgcc gtggggcgcc gtgcgacgga cttctcgatc acctcgtcct
     2521 tcgccatccc gtagccctcg gcgagcgcgt ccgcgacgac acgagcgacg aggctgaccg
     2581 cgcggatcgc gtcgtcgttg cccgggatca cgtagtccac ctcgtccgga tcgcagttcg
     2641 tgtccacgat cgcgatcacg gggatctcga gcttgcgcgc ctcggtcacc gcgatctgct
     2701 ccttcttcgt gtcgaccacg aagacggcgt cagggagtcg ctcgagcgcc tggataccgc
     2761 cgaggttgcg ctcgagcttc tcgcgctcgc gcctgagtcg gatgacctcc ttcttgggga
     2821 ggttgtccat cgcatcggag gactccatct cccgcagctc ccggagccgg accaagcgct
     2881 tggagatggt gctgaagttc gtgagcatgc cgccgagcca tcggttgttc acgaacggca
     2941 tgccgacgcg gcccgcatgc tcctcgacga cctcctgggc ctgcttcttg gtcccgatga
     3001 acaggaccgt gccgcgacgg cggccgagat cacgaacgaa ctcgaacgtc tcctcgatgc
     3061 cggcgaggga cttctcgagg tcgatgatgt agatgccgga tcgctccccg aacaggtagc
     3121 gctgcatctt cgggttccac cgtcttgtct ggtggccgaa gtggacgccg gcctcgagga
     3181 gctcccgcct ggtgacgaca ggcacgtttc cctcctgctg ttggtttctt cctccgtccg
     3241 gatcttcccg cgggcggccc aggtccatct ccgctttctt gggcctggga ccccgccgtc
     3301 gcggtcctcg gacgtgcgtg ctcggcacct tccggcgccg gcgcggggca gtctaccagc
     3361 ggcgatgcga gctcaggccc gacccgttcg caccccacgg ggcctcgacg cgcgccgttc
     3421 cggcgccgcg cggacgggtg catccggcac ctcgcccgcg atcgccaggt atcggatcgc
     3481 gatggccccg atgatcgcgg tgtggaggag gctcaccacg accacgaaga tccatgcgga
     3541 gtacgggggg ttcacgccga gccccgatcc cgggacggac ccgacgatcg tcgccacgga
     3601 ggtcgcgaac cacgggacca cgtagagcac ggcgaaggtc aggttccccg atcccggcag
     3661 gcgagcggct cggagggcgc gaccgaggca gtcgaggaac cggcgcccct ccgtgaccgc
     3721 gatcacgggt gcgaacgcga acacccagac cgcgaggacc aggatcgaga gctgcaggat
     3781 gaacgagaga cccccacctc cgagccccgc cacgatctgc gacgtgaaca acaccgcgac
     3841 accgaaggcg tggatcgcga acgcgacggg gagcgctcgc aaccctcgga tcgctcccca
     3901 ccgactggca cgaccggtct cgacagcctc cacggcgagc cccacgacga tcgcgtgcca
     3961 cagggcgtgc accagagcag ggatcaggac cagcggaagc cccttgccga cgccaaggat
     4021 cccgatcgag accgagaggt cggtggtggt gctgaggggc gcgggcgcca cgatgtgggc
     4081 caggagcgcg aacggccccg ggtagccgag cgcgacgacg acgagccact cgagcaagag
     4141 ccacgccacc gtcgccccga cgacgagcgg actggaccag gttgccgcca ggccacgggc
     4201 gaacgccgtg cggatcggcg gcatcgagtc catcggcggc acggacatcc gcgacaacag
     4261 gccgccccga cggaccggag ccggtcggcc tcgcgtgcca cgggagtccg cgctcatcca
     4321 gcctcctcct cggggtcgag atccgagccg ggtctcggcg tgggaccgag gcgccgttgc
     4381 aacggccatg cgagcgcgag cgcgacgatc gcgaccggaa gaccgccgag cgcgaacccg
     4441 gcgaggagac cgccgagctc gaacgtggtc ggggacggct cgtccgtcag gacaccatcc
     4501 gggtcttccg cgaacgccac gatctcggcg tcctcgacgc ccgtgacagc gacgagaacg
     4561 tccccagcgt cggcgagccc ccggaggaat gcgtctccgg cgtagatacg gaacgcaaga
     4621 tgccccgtcc ggcgatcgat cgcgtagagc gaaccggaat tcgtcggtac gagcaggtgc
     4681 gcgtgattga gcatcggcgg cgtgagtggg gacggatcgt tcaccgcgtg gtcccagagg
     4741 acggtccccg tctcggcgtc cagcccgtac gcgcgtccgg tcacatccac ggcgtagacc
     4801 gtgccgtcgg ccatgacggg cgccagcgtg gagatcccct ggggagagaa gggcgtggtg
     4861 agggggttga cgatctcgga ccgccacagg aaccgaccct ccgccgcctc gagcgcgatg
     4921 acggagccag gctcgaacac gaggatcatc ccgtccgcga ccaccggtcc cgatacgagg
     4981 ttcccctgga ccgcgtcctc gcccgttcgc cagagctcct cgccggtcga acgatcgagc
     5041 gcgaccaccg cccccagcgt ctgttgctcg ccgaccgtgg cgacgagcgc tcggtccccg
     5101 tccaccgtca ccgctccggc gatcgagctg ccgaggtcct tcgaccatcg gagctccccc
     5161 gacgagagct cgacggcggt gacgtgaccg ctgtagtcgc cgacgtaggc ggcggtctcg
     5221 tcggccgtga ccggcgtgcg gacgacgtcc tcgaggggga cggacgaagg ccacaccgtc
     5281 tctcccgacg ggagatcgat cgcgacgagg tgggagcctg tgtgaccctc ctcgcccgga
     5341 ggggccgagg gcgatgcgtt gggggacgcc gtcggagatg cggtggtggc cgggccccgg
     5401 tcgccgaacc cctccgcgaa caccacgacc gggccgtcgg gacccgatcc gatcgccggc
     5461 gtgacggacg gtccgaggtc ccgcggcgcc gtgaagacct ccgagccgtc ttcggcgtcg
     5521 aagccgaaaa cctcgtgcgg ccctacgacc acgatcgtgc cgtcgtcacc gacgatcggt
     5581 gaggagagac ctccggtcac gtccgcatcg tcgatcgcga tgtcgcggtg acgccaggcc
     5641 acctccagcg gagcggaggg tccgtctgac agaccgtcgt gggacggccc gccttggaac
     5701 tgcggccact cggcgagcgc tacgagcggc atgccgagca ccacggcgag ggtgcacccg
     5761 gccagggtca tccaacgtcg gggcacacgg gaacggtgag cagcgcgcaa ggcgcgtcat
     5821 cctaaccggt cgcctgcgac ggggcgcctc aggactccag cggcgcgagg cggatgaagc
     5881 gccagacctc gaccgggccg aggtattcga gcgggtcgac gtaggtgtcg gcgagcttca
     5941 cgccgaagtg aaggacggga acgatgtcgc ccgcatgccc tgtccccgag cgggcgatcg
     6001 cctgccctgg tgacacgaca tcgccccttc ggaccgcgac agagtccagg aacgagtagg
     6061 tcgactcgag gccggcgccg tggtccacgg tgacgaacag ccgcccgcct accggcccgg
     6121 cgaacgtgac cgtgcccgtg gtcgctgcac ggacgggtgt accgaccggc gcggcgatgt
     6181 cgatcccgcg atggcccgat ccgaacggag aatcgggcgg atcgaacccg cggaccacgg
     6241 gagcgacgat cggccaggtc caatcccccg aggcgtgcgc cggcgcaggc acggcggata
     6301 cgaggaccgc actccacacc ccacaccaca gaacgagctt cctcacgagc aggacgctag
     6361 gaggtccggc cggcgcgcgc cgtgaagcgc gtcacacggg aagtgggtca gtcgaactcg
     6421 gagagctggc cgcggagccc gccgacggcc ttcgtgtgga tctgacacac acgcgactcg
     6481 gtcacgccca agatctcccc gatctcggcg agcgtcaggc cctcgaagta gtagagcgtc
     6541 acgactatct tctcgcgctc ggagagggag ttgatcgccg cggccagcat cccgcgggtc
     6601 tcctggctct ccacgcccca cgtcggatcg atc
//
LOCUS       DASUVE010000326         2035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_726519, whole genome shotgun sequence.
ACCESSION   DASUVE010000326 DASUVE010000000
VERSION     DASUVE010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726519"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VF036_07060"
     CDS             <1..360
                     /locus_tag="VF036_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX6265023.1"
                     /translation="FREGGGDRAPVAVQVHVSWAEDEETALAIAHDQWRTNVFGSDLA
                     WNLELPAQFDEAATHVRPDAVTETVLVSADLGQHAKWLLELADLGVDAIYLHHVGKEQ
                     DAFIEAFADHVLPELVS"
     gene            357..2030
                     /locus_tag="VF036_07065"
     CDS             357..2030
                     /locus_tag="VF036_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEX6265024.1"
                     /translation="MTTARTSDLWWKNAIIYCLDVETFLDWDGDGTGDLVGLTERIDY
                     LAGIGISCLWLMPFQPSPNRDDGYDITDYYGVDPRLGSLGDFVAMIRTAKDRGMRVIV
                     DLVVNHTSDKHPWFRAARSSPTSEFRDYYVWADEPPAEPKAEVVFPDQEDTIWAWDEK
                     AGQYYLHHFYSHQPDLNIANPKVREEMARIVGFWLELGVDGFRVDAVPFLLETGGIAG
                     EVELDPHDFLRDLRSFMSRRRGDVMMLGEVNLPHKDLQLFFGGPDGDEVQLLFNFILM
                     QSIYLSLARQDARPLAKALRETVRKTDDQQYANFLRNHDELTLDKLTDAQRQEVFDAF
                     GPEEDMQLFGRGLRRRLPSMLDGDEQRIRMTYSLLFSLPGTPVLFYGEEIGMAENLEI
                     EGRLSVRSPMQWTDEKNGGFSSAPPSRLRRPVVEGKFGPMAVNAAAQRRDPDSLLTWM
                     ERVIRRRRETTELAWGSWQVLETDVDAVLAHRCDWEGRSVVAVHNLGADPCVVRVELG
                     DVPEGATLDDLLDERPALDEIDGTSLELQLGGYGYRWYRIGTPDQMTPP"
BASE COUNT          329 a          691 c          695 g          320 t
ORIGIN      
        1 ttccgcgagg gaggcggcga tcgcgcgccg gtcgccgtgc aggtccacgt ctcctgggcg
       61 gaggacgagg agacggccct cgcgatcgcg cacgaccagt ggcggacgaa cgtgttcggg
      121 tcggacctcg cgtggaacct cgagctgccg gcccagttcg acgaggccgc cacccatgtg
      181 cggcccgacg ccgtgacgga gacggtcctg gtctcggccg acctcggtca gcacgcgaag
      241 tggctcctcg agctcgcgga cctcggcgtg gatgcgatct acctccacca cgtcgggaag
      301 gaacaggacg ccttcatcga ggcgttcgcc gaccacgtcc tcccggagct ggtgtcgtga
      361 cgaccgcccg cacgagcgat ctgtggtgga agaacgcgat catctactgc ctcgacgtcg
      421 agacgttcct cgattgggac ggggacggca ccggcgacct cgtcggcctc accgagcgga
      481 tcgactacct cgccgggatc gggatcagct gtctgtggct gatgcccttc cagccctcac
      541 cgaaccgaga cgacggctac gacatcacgg actactacgg cgtcgacccg cgcctcggct
      601 cgctcggcga cttcgtcgcg atgatccgca ccgcgaagga ccgcgggatg cgtgtgatcg
      661 tcgacctcgt ggtgaaccac acgtcggaca agcacccatg gttccgcgcc gcgcgctcct
      721 cccccacctc ggagttccgc gactactacg tctgggcgga cgagccgccg gccgagccga
      781 aggcagaggt cgtgttccct gatcaggagg acacgatctg ggcgtgggac gagaaggccg
      841 ggcagtacta cctccaccac ttctactccc accagccgga cctgaacatc gcgaacccga
      901 aggtccgtga ggagatggcg aggatcgtcg ggttctggct cgaactgggc gtcgacggct
      961 tccgcgtgga cgccgtgccg ttcctcctcg agaccggcgg gatcgcgggc gaggtggagc
     1021 tcgaccccca cgacttcctc cgggacctcc ggtcgttcat gtcccggcgc agaggcgacg
     1081 tgatgatgct cggcgaggtg aacctgcccc acaaggatct gcagctgttc ttcggcggcc
     1141 cggacggcga cgaggtccag ctgctcttca acttcatcct gatgcagtcg atctacctct
     1201 cgttggcgcg ccaggacgcc cgtccgctgg cgaaggcgct ccgcgagacg gtgcggaaga
     1261 cagacgacca gcagtacgcg aacttcctcc gcaaccacga cgagctgacg ctcgacaagc
     1321 tgaccgacgc ccagcgccaa gaggtcttcg acgcgttcgg tccggaggag gacatgcagc
     1381 tgttcgggcg tgggctccgt cggcgcctcc cctcgatgct cgacggcgac gagcaacgca
     1441 tccgtatgac ctacagcctg ctgttctcgc tcccgggcac gccggtgctg ttctacggcg
     1501 aggagatcgg gatggccgag aacctcgaga tcgagggacg gttgagcgtc cgctcaccga
     1561 tgcagtggac cgacgagaag aacggtgggt tctcctcggc tccaccgtcg cgtctgcgcc
     1621 gtccggtcgt cgagggcaag ttcgggccga tggcggtcaa cgccgcggcg caacgacgcg
     1681 accccgattc cctgctgacc tggatggagc gggtcatccg gcgccggcgc gagacgaccg
     1741 agctcgcgtg gggctcctgg caggtcctgg agaccgacgt cgatgcggtc ctcgcgcatc
     1801 ggtgcgactg ggagggtcgg agcgtcgtcg cggtccacaa cctgggcgcg gacccgtgcg
     1861 tcgtgcgcgt ggagctcggc gacgtgcccg agggcgcgac gctcgacgat ctgctcgacg
     1921 agcggccggc gctcgacgag atcgacggca cttccctgga gctgcagctg ggcggctacg
     1981 gctaccgctg gtaccggatc gggacgccgg accagatgac ccctccttaa gggtc
//
LOCUS       DASUVE010000327         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_160846, whole genome shotgun sequence.
ACCESSION   DASUVE010000327 DASUVE010000000
VERSION     DASUVE010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160846"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VF036_07070"
     CDS             complement(<1..352)
                     /locus_tag="VF036_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265025.1"
                     /translation="MSVPREERSTADRRIDIIAAVLLAVAAVATAWSSYQASRWTGEQ
                     AKAFATASASRVESTRSSNLANAQTQIDVAVFIQWVDARLQGEADLVAFYEARFRDEF
                     KPAFRAWLRTDPFED"
     gene            complement(349..1173)
                     /locus_tag="VF036_07075"
     CDS             complement(349..1173)
                     /locus_tag="VF036_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265026.1"
                     /translation="MRWALGALIVFGVSAGMIVAILRVRRRAPEGGYFDDGDRAAGVF
                     GVLATGFALLLGFVIFLAFTKYDGSRAGAEAEALAVIQLFETSQLMPPDARPLLAGEV
                     ECYARSVVDLEWPAMQAGRGFETINPWGLSMFRTLQAIEPATAAEQSAYDAWLAQTSA
                     REEARRDRLHAAEGVVPQPLWVVLLLSAVLVLVYVLFFADSGERAVVQGLMAGSVTAV
                     VVASLLVLSSLNRPYDTDVGGIRPVAMQRSLETIASARAALDLDEPLPCDADGQPL"
     gene            complement(1268..>2303)
                     /locus_tag="VF036_07080"
     CDS             complement(1268..>2303)
                     /locus_tag="VF036_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX6265027.1"
                     /translation="QFHHVIDVAATMLEVAGLPEPQAVDGISQAPIEGVSMAYTFDDP
                     AAPDRHETQYFEMFGNRGIYHKGWTAVTKHKTPWIMVGGPAIAFDDDVWELYDTGKDW
                     SQARDLSEEHPDTLHELQRLWLIEATRYNVLPLDDRIAERILADLAGRPTLIQGNRQL
                     LFGGMGRLTESSVVSIKNKSHAVTAELVVPEGGAEGVIVAQGGSIGGWSLYVKDGKPR
                     YCYNLLGVQRFYVDSDHELQPGTQQVRMEFAYAGGGLGKGGNVTLLVDGQPVGKGDVA
                     ATAAMIFSADDTCDVGMEGGALIAEDYPVPNNFTGEINWVAIDVAEAAEDADHLISPE
                     ERLRIAMARQ"
BASE COUNT          382 a          798 c          747 g          376 t
ORIGIN      
        1 catcctcgaa cggatcggtg cggagccatg ctcgaaaggc gggcttgaac tcgtcgcgga
       61 accgcgcctc gtagaacgca acgaggtccg cctctccttg gagacgggca tcgacccatt
      121 ggatgaacac cgcgacgtcg atctgcgtct gggcgttcgc gaggttcgac gaccgggtgg
      181 attcgacccg cgaggcgctg gccgtggcga acgccttcgc ctgctcgccc gtccaacggc
      241 tggcctggta gctgctccac gccgtggcga ccgccgccac cgcgagcagg acggccgcga
      301 tgatgtcgat ccgtcgatct gccgtcgaac gttcttctcg gggaacgctc acaacggctg
      361 cccgtccgca tcgcagggga gcggctcatc gaggtcgagc gcggcgcgag cggacgcgat
      421 ggtctccagg gatcgctgca tcgcgaccgg gcggatcccg ccgacgtccg tgtcgtaggg
      481 tcgattgagc gacgacagga cgagcaggga cgcgacgacc accgcggtca ctgagccagc
      541 catcaacccc tggaccacgg cgcgttcacc gctgtcggcg aagaagagca cgtagacgag
      601 gaccaacacg gcggacagga gcagcaccac ccacaacggc tggggaacga ccccttccgc
      661 cgcgtggaga cggtcacgcc gtgcttcctc ccgtgcgctg gtctgggcga gccaggcgtc
      721 gtaggcgctc tgctccgcgg cggtcgccgg ttcgatcgcc tggagcgtgc ggaacatcga
      781 gagcccccac ggattgatcg tctcgaaccc gcgccccgct tgcatcgccg gccactcgag
      841 gtccaccacg gatcgcgcgt agcactcgac ctcgcccgcg agtaggggac gcgcgtccgg
      901 cggcatcagt tgggaggtct cgaagagctg gatgaccgcc agcgcctctg cctcggcgcc
      961 cgcacgcgac ccgtcgtact tggtgaacgc gaggaagatc acgaagccga gcaacagagc
     1021 gaatcccgtc gcgagaacgc cgaatacccc ggccgcgcgg tcaccgtcgt cgaagtaccc
     1081 accctcggga gcgcgacggc gcacgcggag gatcgccacg atcatccccg cgctcacccc
     1141 gaagacgatc agcgctccga gagcccacct catgccgacc tccggctgtc cgggctcacg
     1201 atggtgtcaa gggaaccgag tgcgggcaga gatggctaga cgatccttcc tctcgcacac
     1261 ccgatcgtca ttggcgggcc atcgcgatcc gcagacgttc ctccggcgag atcagatggt
     1321 cggcatcctc ggcggcctcg gccacgtcga tcgcgaccca gttgatctcg cccgtgaagt
     1381 tgttgggcac gggatagtcc tcggcgatga gcgcgccacc ttccatcccg acatcacacg
     1441 tgtcatccgc ggagaagatc atcgccgccg tcgcggccac gtctcccttc cccacgggct
     1501 gaccgtcgac gaggagcgtg acgtttccgc ccttgccgag ccctccaccg gcgtaagcga
     1561 actccatgcg cacctgctgt gtaccgggct ggagctcatg gtcgctgtcg acgtagaagc
     1621 gctggacccc caggaggttg tagcagtaac ggggcttccc atctttcacg tagaggctcc
     1681 aaccaccgat gctgccccct tgggcgacga tgacgccctc ggcgccccct tccggtacga
     1741 cgagctcagc ggtcaccgcg tgggacttgt tcttgatgct gacgacggaa ctctcggtca
     1801 gccggcccat cccaccgaag aggagctggc ggttgccctg gatcagcgtt ggccgaccag
     1861 cgaggtcagc gaggatccgc tccgcgattc gatcgtcgag cgggagcacg ttgtaccgcg
     1921 tggcttcgat gagccagagc cgctggagct cgtgaagcgt gtccggatgc tcctcggaca
     1981 gatcgcgtgc ctggctccag tccttcccgg tgtcgtagag ctcccagacg tcgtcgtcga
     2041 acgcgatggc cggcccgccg accatgatcc acggcgtctt gtgtttggtc acggccgtcc
     2101 accccttgtg gtagatgccg cggttgccga acatctcgaa gtactgcgtc tcgtggcggt
     2161 ccggcgccgc ggggtcgtcg aaggtgtagg ccatgctgac gccttcgatc ggcgcttgtg
     2221 agatcccgtc caccgcctgc ggctcgggca gcccggccac ttccagcatc gtcgccgcca
     2281 cgtcgatgac gtgatggaac tgg
//
LOCUS       DASUVE010000328         6674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_729058, whole genome shotgun sequence.
ACCESSION   DASUVE010000328 DASUVE010000000
VERSION     DASUVE010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729058"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VF036_07085"
     CDS             complement(<1..237)
                     /locus_tag="VF036_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6265028.1"
                     /translation="MAQPLLEVKDLKVHFPTDDGLVKAVDGVSYSLAAGETLGIVGES
                     GSGKSVSSLTVMGLINPKQATISGEVFFQGQDLLK"
     gene            complement(237..1271)
                     /locus_tag="VF036_07090"
     CDS             complement(237..1271)
                     /locus_tag="VF036_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6265029.1"
                     /translation="MGRYLIRRALFMILVIIIVSLLTFLIFVKLPAGDPARRAVGRTT
                     TPQQIENARRAFGLDKPLYIQYWRFLRGFIPWPPGDPVLMLAGHPPRSHEGLWLNEDV
                     YYSYDGFVPVREELARRIPITGTLAIGAAVIWLLLGIPIGILSGIRRRSVADRAAMIF
                     AIIGVSMPIFWLAQLLLYAFWYKLELLPESGIPIGYSGWGGAIRAALEGRFILPWVSL
                     AYINAAFYARMVRGNLIETMNEDYIRTARAKGLSERRVIYKHGLRGALTPVVTMLGLD
                     IGLLLGGAFITETVFGLPGIGQKAVQAIGTNDFPTVMGVTILGAFIIAVANLVVDVAY
                     AFVDPRVRYT"
     gene            complement(1366..3237)
                     /locus_tag="VF036_07095"
     CDS             complement(1366..3237)
                     /locus_tag="VF036_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6265030.1"
                     /translation="MKRVLRLIAVVTVLGIVAASCGGDEEPPPGGDTPSDSKELAGGT
                     LTMAMLADVTAAFDPQKEYYSVTWEYYRCCLLRNLMSYEGVPTDEGGADIHPDLAAGE
                     PEQSSDGLTWTYTIKPGIHYAPPKEDVEITAQDFIRALERTANPDANVGGYSFYYSVI
                     EGFDDFAAGDAEEISGLNAVDDHTLEITTTSPVGDMSYRLAMGTAAPIPPDDEGERMG
                     VAEGHDRNYGRFLVATGPYMFEGSETMDFSADPKDQTEAPGYVPGRQIVLVRNPSYDA
                     TTDGLRDAYPDRIEVRIGGDNDDLYNQVASGTLDFVVDGVVPAEKIREYQTDPALQDK
                     INIYASDAIRYVSFNLAVPPFDDVHVRKAVSWAFDKQGFRQLRGGPSVGEIAGHIMVN
                     SLENNLLADYDPYQTTNASGDPEKAKEEMALSAYDSDGDGVCDDPVCQDILTITDRED
                     PYPDQMALLKQFLEPLGMTLDDKQLERGTMYNQCNDANSHHALCAGPGWGKDYADGVT
                     FGEPLFTSAGLWESCCNYSLLGASPEQLTEWGYDVTEVPSVDDMEDECAALEPGDERF
                     QCWAEFDRHLMEEVVPMVPYLFDNSVDILSENIVNYSFDQFAGLAAFDSMAVPGNTG"
     gene            complement(3334..4380)
                     /locus_tag="VF036_07100"
     CDS             complement(3334..4380)
                     /locus_tag="VF036_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6265031.1"
                     /translation="MSRSDTDTDVSQAEQREAAERTDLEAAAPSAETPASIIGRSPWE
                     IFWSHFRQDKFALMGLVIIGLMILMAVFAPLVASWSGHEPNQVNLEALDEFGLPSAPT
                     WWPGPEAKAPPKYFLGVDDTGRDLFVRIAYGARTSLTVAFFATGIAVVIGVLLGLTSG
                     FYRGKIDTVISRATDVVLALPILLLALGIASACGANQEGCFWGFIKPGLRPVILIIGL
                     FGWPYIARIVRGQVLSIREKEFVEASRSLGASNRRIILREILPNVTAPIIVYTTLIIP
                     SNILFEAGLSFLGVGVPDGTPSWGAMLADAGTAFRFAPWLMIFPGLALFLTTLAFNLV
                     GDGLRDALDPRKAV"
     gene            complement(4484..5254)
                     /gene="tpiA"
                     /locus_tag="VF036_07105"
     CDS             complement(4484..5254)
                     /gene="tpiA"
                     /locus_tag="VF036_07105"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017535440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX6265032.1"
                     /translation="MAERTPMIAANWKMHKTHLEAIQAVQKLSYLLDRKDAERVEVVI
                     CPAFTALRSVQTLIDSDRLPYGLGAQDIHPEDEGAFTGAVAGPMLAALDVGYVIVGHS
                     ERRQLFGEDDATVNRKVKAVIRNGMAPIVCVGESLEERESGGTETKVTEQVQRAFVGI
                     DAAAAAGSVVAYEPIWAIGTGRNAEPTDAGQVVEVIRATLADRVSTELADAARVLYGG
                     SVKPGNIREFMAHPEIDGALVGGASLDPESLALIARYR"
     gene            complement(5256..6446)
                     /locus_tag="VF036_07110"
     CDS             complement(5256..6446)
                     /locus_tag="VF036_07110"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX6265033.1"
                     /translation="MALRTLDDLGDVAGSRIFVRVDFNVPLDGGRVADDLRIRAAIPT
                     LRELRERGAALVVASHLGRPEGEARDDLRLAPVADRLGELLEVEVMALDEVVGDGPEA
                     VCAHIEQGDVVLLENLRFDPGEEANDVRFAGRLAELADAYVDDAFGAAHRAHASVVAL
                     PDLMRASGRPTAAGRLLQREVEVLGRLLRDPDRPYVAVLGGAKVSDKLGVLGALAERA
                     DALLIGGAMAFTLIAAEGGEIGDSLVERDRFDEVRAVMRGTGDALIELPQDVVAATEA
                     SADVRPQTVPAREIPHGLKGLDIGPRTVEEFTRILADAKTILWNGPMGVFELEPFSAG
                     TRGVAVAIAGAAAFSVVGGGDSLLAVKREGLEDSFDHLSTGGGASLEFLEGAPLPGIA
                     VLED"
     gene            complement(6446..>6674)
                     /locus_tag="VF036_07115"
     CDS             complement(6446..>6674)
                     /locus_tag="VF036_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX6265034.1"
                     /translation="AYRAASETDRLKGKLVYTEDPIVSSDIVGSPASCTFDSLSTMAM
                     GNMVKVMGWYDNEWGYSNRLVDLVNLVASKL"
BASE COUNT         1085 a         2277 c         2246 g         1066 t
ORIGIN      
        1 cttcaggagg tcctgaccct ggaagaagac ctcgccggag atggtcgcct gcttggggtt
       61 gatcaggccc atcaccgtga gggagctcac gctcttgccc gagccagact ccccgacgat
      121 ccccagcgtc tccccggccg ccagtgagta gctcaccccg tccacggcct tgacgaggcc
      181 gtcgtcggtc gggaaatgga ccttgaggtc cttcacttcg agcagtggct gcgccactag
      241 gtgtacctca ccctcgggtc cacgaacgcg tacgcgacgt cgaccaccag gttcgcgacc
      301 gcgatgatga acgcgccgag gatcgtgacg cccatcacgg tcgggaagtc gttcgtgccg
      361 atcgcctgaa cggccttctg cccgatcccc gggagcccga agaccgtctc ggtgatgaag
      421 gcgccgccga gcaggagacc gatgtcaagg ccgagcatcg tcacgaccgg cgtgagggcc
      481 ccgcggaggc cgtgcttgta gatcacgcgg cgctcggaca ggcctttggc ccgggccgtc
      541 cggatgtagt cctcgttcat cgtctcgatc aggttcccgc gcaccatgcg agcgtagaag
      601 gccgcgttga tgtacgcgag ggagacccag ggcaggatga accggccctc gagggcggca
      661 cggatcgccc ctccccagcc gctgtacccg atcgggatcc cggattcggg taacagctcg
      721 agcttgtacc agaacgcgta gaggaggagc tgcgcgagcc agaagatcgg catggagacg
      781 ccgatgatcg cgaagatcat ggcggcgcgg tcggcgacgg atcttcgtcg gatgccggac
      841 aggatgccga tcgggatgcc gaggagcagc cagatcacgg cggcaccgat cgcgagcgtc
      901 ccggtgatgg ggatccgccg ggccagctcc tcccgcacgg ggacgaagcc gtcgtaggag
      961 taatagacgt cctcgttgag ccagagcccc tcgtggctcc gcggggggtg gccggccagc
     1021 atgagcacgg gatcgcccgg cggccacggg atgaaccccc ggaggaaccg ccagtactgg
     1081 atgtagaggg gcttgtcgag accgaacgcc ctgcgcgcgt tctcgatctg ctggggcgtg
     1141 gtggtgcggc cgaccgcccg gcgggccggg tccccggccg ggagcttcac gaagatgagg
     1201 aacgtgagga gcgagacgat gatgatcacg aggatcatga agagggctct ccggatcagg
     1261 tatcggccca tccgtctgct cctccctccg gccccctcgc gggagcctcg gacggaacgg
     1321 tgagggggcc gctcgcgcga ccccctcgac cgtctggatc aggtgctatc cggtgttgcc
     1381 gggcacggcc atcgagtcga acgccgccag accagcgaac tggtcgaacg agtagttgac
     1441 gatgttctcc gagaggatgt cgacgctgtt gtcgaacagg tacggcacca tcggcacgac
     1501 ctcttccatc aggtgtcggt cgaactccgc ccagcactgg aaccgttcgt cacccggctc
     1561 caacgctgcg cactcgtcct ccatgtcgtc caccgacgga acctccgtca cgtcgtagcc
     1621 ccactcggtc agctgctcgg gcgaggctcc caggagcgag tagttgcagc agctctccca
     1681 cagacccgcg gaggtgaaca ggggctcgcc gaacgtgaca ccgtcggcgt agtccttccc
     1741 ccaaccgggg ccggcgcaga gcgcgtgatg cgagttcgca tcgttgcact ggttgtacat
     1801 cgtgccgcgc tccagctgct tgtcatcgag cgtcatcccg agcggctcga ggaactgctt
     1861 caggagggcc atctgatccg ggtacggatc ctcgcggtcg gtgatcgtga ggatgtcctg
     1921 gcataccgga tcatcacaga ccccgtcccc gtccgagtcg tacgcggaca gggccatctc
     1981 ctccttggcc ttctcggggt caccggacgc gttcgtggtc tggtacgggt catagtcggc
     2041 caggaggttg ttctcgaggc tgttgaccat gatgtgtccc gcgatctcac cgaccgaggg
     2101 accaccccgc aactgccgga acccctgctt gtcgaacgcc cagctgaccg ccttgcggac
     2161 gtggacgtcg tcgaagggag gtaccgccag gttgaaggac acgtaccgga tcgcgtctga
     2221 tgcgtagatg ttgatcttgt cctgcagcgc ggggtccgtc tggtactcgc ggatcttctc
     2281 cgcgggcacg acgccatcga ccacgaagtc gagggtcccc gacgcgacct ggttgtacag
     2341 gtcgtcgttg tcgccgccga tccgaacctc gatccggtcc gggtaggcgt ctcggagtcc
     2401 gtccgtcgtc gcatcgtagg acgggttgcg gaccaacacg atctggcgac ccgggacgta
     2461 gcccggggcc tcggtctggt ccttcgggtc cgccgagaag tccatcgtct cggacccctc
     2521 gaacatgtac gggcccgtcg ccacgaggaa gcggccgtag ttccggtcgt gcccctcggc
     2581 gacacccatc cgctccccct cgtcgtcggg cgggatcggc gctgcggtgc ccatggcgag
     2641 ccggtagctc atgtcaccga ccggtgaggt cgtggtgatc tcgagcgtgt ggtcgtcaac
     2701 ggcgttcagc ccggagatct cttcggcgtc gcccgcggcg aagtcgtcga agccctcgat
     2761 caccgagtaa tagaaggagt acccgccgac gttcgcgtcg gggttcgccg tccgctcgag
     2821 cgcccggatg aagtcctgcg ccgtgatctc cacgtcctcc ttcggaggcg cgtagtggat
     2881 gccgggcttg atcgtgtagg tccaggtcag tccgtccgag gactgctccg gttcgccggc
     2941 agcgaggtcc gggtggatgt cggcgccgcc ctcgtccgtc gggacgccct cataggacat
     3001 caggttccgg agcagacagc agcggtagta ctcccacgtg acggagtagt actccttctg
     3061 tgggtcgaac gccgcggtga cgtcggcgag catcgccatg gtcagcgtgc cgcccgcaag
     3121 ttccttcgag tcactcggcg tgtcgccgcc cggaggcggt tcttcgtcgc caccacacga
     3181 agcggcgacg atcccgagga ccgtcacgac ggcgatcaat ctgagtactc gtttcaaagc
     3241 tacccccctt ctcatggtgc agatacaacg atcaatggtg ctccgtgctc gaggtcctcc
     3301 tcctttcgaa ccgggttccc cctgtcgata cgctcagacg gccttccgag gatcgagtgc
     3361 gtctcggagg ccgtccccca cgaggttgaa ggccagcgtc gtgaggaaca gggcgaggcc
     3421 cgggaagatc atcagccagg gagcgaatcg gaaggccgtg cccgcgtcgg cgagcatggc
     3481 tccccaggac ggtgtcccgt ccgggacccc cacgccgagg aacgagagtc cggcctcgaa
     3541 caggatgttg ctcgggatga tcagcgtggt gtagacgatg atcggcgcgg tcacgttcgg
     3601 gaggatctcg cggaggatga tgcgccggtt ggaggctccc agcgagcggg aggcctccac
     3661 gaactccttc tcacggatcg acagcacctg gccgcggacg atgcgagcga tgtacggcca
     3721 tccgaacagc ccgatgatca agatcaccgg tcgcaacccc ggtttgatga agccccagaa
     3781 gcagccctcc tggttcgcgc cgcacgcgga ggcgatcccg agcgccagga gcaggatcgg
     3841 caacgcgagc acgacgtcgg tggcgcgcga gatgaccgtg tcgatcttgc cccggtagaa
     3901 gcccgacgtc agaccaagca ggacaccgat cacgaccgcg atgcccgtcg cgaagaacgc
     3961 gaccgtgaga gacgtgcgtg cgccgtacgc gatccgcacg aagaggtcac gtccggtgtc
     4021 gtcgaccccc aggaagtact tgggaggggc cttcgcctcc gggcccggcc accaggtggg
     4081 cgccgacggg aggccgaact cgtcgagcgc ctccagattg acctggttcg gttcgtgccc
     4141 ggaccagctg gccaccagcg gtgcgaagac cgccatcagg atcatcagcc cgatgatcac
     4201 gagccccatc agggcgaact tgtcctggcg gaagtgactc cagaagatct cccagggact
     4261 ccggccgatg atcgaggccg gggtctcggc cgacggcgcg gcggcctcca ggtcggtgcg
     4321 ctcggcagct tcgcgctgtt ccgcctggga tacgtcggtg tccgtgtcgg atcgactcat
     4381 gcccgggaac cgatactaga gaggttgcaa gggacccgca atccagcaac cctctgaggg
     4441 gcacccccgc ggcggctttg atgggcaagg aagcgaaagc gcttcaccgg taccgggcga
     4501 tcagggccag gctttccggg tcgaggctcg cgcccccgac caacgccccg tcgatctcgg
     4561 ggtgggccat gaactcccgg atgttgcccg gcttgacgct gcctccatac aggacacgag
     4621 ccgcatcggc gagctccgtg gacacgcggt ccgcgagggt ggcccggatc acctccacga
     4681 cctgtcccgc gtcggtgggt tccgcgttcc gtcccgtccc gatcgcccag atcggctcgt
     4741 aggcgactac ggacccggcg gcggccgccg cgtcgatccc gacgaaggcc cgctggacct
     4801 gctcggtcac cttcgtctcg gtgccgcccg actctcgctc ctcgagcgac tcccccacgc
     4861 agacgatcgg cgccatcccg ttgcggatca cggccttcac cttccggttg accgtcgcgt
     4921 cgtcctcgcc gaagagttgg cggcgctcgg aatgcccgac gatgacgtag ccgacatcga
     4981 gcgccgcgag catcggcccc gccaccgccc cggtgaacgc gccctcgtcc tcgggatgga
     5041 tgtcctgcgc gcccaacccg tagggcaagc gatcggagtc gatcagagtt tggacggaac
     5101 ggagggcggt gaacgcgggg cagatcacca cctccacgcg ctcggcgtcc ttccggtcga
     5161 gcaggtacga gagcttctgc acggcctgga tcgcctcgag gtgcgttttg tgcatcttcc
     5221 agttcgcggc gatcatcggc gtccgctcgg ccacgtcaat cctccaggac cgcgatgccg
     5281 gggagcggtg ccccctcgag gaactcgagc gaggcgccgc cgccggtgga caggtggtcg
     5341 aacgaatcct cgagcccctc ccgcttgacc gcgagcaagg agtccccgcc gccgaccacc
     5401 gagaacgcgg ccgcgcccgc gatcgccacg gcgacacccc gggtgcccgc cgagaacggc
     5461 tcgagctcga agacgcccat cggaccgttc cacaggatcg tcttggcgtc ggcgaggatc
     5521 cgggtgaact cctccacggt ccggggtccg atgtcgagtc ctttgaggcc gtgcgggatc
     5581 tcgcgcgccg gcacggtctg cgggcggacg tcggcgctcg cctcggtggc ggcgacgacg
     5641 tcttgcggga gctcgatcag ggcgtcgccc gtcccccgca tgaccgcacg gacctcgtcg
     5701 aagcgatcgc gctccaccaa gctgtcgccg atctcgcccc cctccgccgc gatcagcgtg
     5761 aacgccatcg ctccgccgat cagcagcgcg tcggcgcgct cggccagggc gccgagcacg
     5821 ccgagcttgt ccgagacctt ggcgccgccg aggacggcga cgtacggccg gtcggggtcg
     5881 cggaggagtc ggccgaggac ctcgacctca cgctgcagca gccgtccggc cgccgtcggc
     5941 cggcccgacg ctcgcatcag gtcgggcagc gcgacgacgc tcgcgtgcgc ccgatgcgcg
     6001 gcgccgaacg cgtcgtccac gtacgcgtcg gcgagctcgg cgaggcgtcc tgcgaagcgg
     6061 acgtcgttcg cctcctcgcc cggatcgaag cggaggttct cgaggaggac cacgtcgccc
     6121 tgctcgatgt gggcacagac ggcctcgggg ccgtcgccga cgacctcgtc gagcgccatc
     6181 acctccacct cgagcagctc cccgagccgg tcggcgacgg gcgcgagccg gaggtcgtcc
     6241 cgcgcctccc cctccggacg tccgaggtgg gacgccacca cgagggccgc tccccgctcg
     6301 cgcagttcgc ggagggtggg gatcgcggca cggatccgca gatcgtccgc cacgcggcca
     6361 ccgtcgagcg ggacgttgaa gtcgacgcgg acgaagatcc gggagccggc gacgtcgccg
     6421 aggtcgtcga gcgtccggag cgccactaga gcttggaggc gaccaggttc acgaggtcga
     6481 ccaggcggtt cgagtacccc cactcgttgt cgtaccaacc catgaccttg accatgttcc
     6541 ccatcgccat cgtggagagg gagtcgaagg tgcacgaagc gggcgacccc acgatgtcgc
     6601 tcgagacgat cggatcctcg gtgtagacca gcttgccctt cagccggtcg gtctcggacg
     6661 cggctcgata ggcg
//
LOCUS       DASUVE010000329         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_915899, whole genome shotgun sequence.
ACCESSION   DASUVE010000329 DASUVE010000000
VERSION     DASUVE010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915899"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VF036_07120"
     CDS             <1..1161
                     /locus_tag="VF036_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6265035.1"
                     /translation="SYEGLSIAHSGSIGAPAVWDALGEEGKSSLLIGVPPGFPPPKEF
                     PGWRVGCFLTPPSAEHFAYPQELEVEIEEELGGKGQYIFDITNFRKQGMDFVLDQVFK
                     MTERRFKVARRLVKEKPWDFFMLVEMGMDRLHHVFWHYFDPKHPLYKPGNPFEDAFQR
                     YYRALDDEVGALTDLLPEDVITIVMSDHGARPMMGGICFNDWLIEHGYLVLKEKVSGV
                     TPVARTAIDWGRTTAWGDGGYYGRLFLNVAGREPQGSIRPAEYETVRDELVTKLEAMP
                     GPDGALLGTKVHKPQDVYPEVKGVAPDLLVYFGDLEWRSVGSVGNPSIYTFENDIGPD
                     GANHDRTGVFAMKGAPGQPSGKVDGLSLVDVGPTILSLYGIDSHGAASRSFL"
     gene            1161..1793
                     /gene="cysC"
                     /locus_tag="VF036_07125"
     CDS             1161..1793
                     /gene="cysC"
                     /locus_tag="VF036_07125"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX6265036.1"
                     /translation="MTTEQHGFTVWFTGLSGSGKSTIAEMLFHEFQARGLKTEILDGD
                     VVRQNLSKGLGFSKEDRDTNILRIGFVADLLTRNGVATICCPISPYKAARDAVRAQIG
                     DAFVEVYVHATVAEIAANRDPKGLYKKALAGEITGFTGVDDPYEAPESPELVVDTLVE
                     TPEQSLQKVMDRLAELGHIEDATLQVEGDRVHSGMTDLRITESGHVAEEH"
     gene            1823..2362
                     /locus_tag="VF036_07130"
     CDS             1823..2362
                     /locus_tag="VF036_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6265037.1"
                     /translation="MDIPRSPDPVSEPKAYQAHLLSLLGADDPAHAQAEAAARWRSLF
                     TEAADRRDVRPEPSEWSVVECLGHAVDAEVVSAARYRWVLAHDEPPLIGYDQDLWVDT
                     LHADRRDDPEELLGLFEPLRAANLALWSRSSEDDRARVGVHAERGRESFDLLFRMIAG
                     HDRFHLAQARRALDAVTEP"
     gene            2469..>2595
                     /locus_tag="VF036_07135"
     CDS             2469..>2595
                     /locus_tag="VF036_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007147229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEX6265038.1"
                     /translation="MWLEYEIGIGKTARRAYGFDEVAIVPSRRTRDPDDVDISWEV"
BASE COUNT          436 a          848 c          907 g          404 t
ORIGIN      
        1 agctacgagg gcctgagcat cgcgcactcg ggatcgatcg gtgcccccgc cgtctgggac
       61 gccctcggcg aggagggcaa gtcctcgctg ctgatcggcg taccccccgg cttcccgccg
      121 ccgaaggagt tcccgggctg gcgcgtgggg tgcttcctca cgccgccgtc ggccgaacac
      181 ttcgcctatc cccaggagct cgaggtcgag atcgaagagg agctcggggg gaaggggcag
      241 tacatcttcg acatcacgaa cttccgcaag caggggatgg acttcgtgct cgatcaggtg
      301 ttcaagatga ccgaacgccg cttcaaggtg gcgcgccgcc tcgtgaagga gaagccgtgg
      361 gacttcttca tgctggtcga gatgggcatg gaccggctcc accacgtctt ctggcactac
      421 ttcgacccca agcacccgct ctacaagccc gggaacccat tcgaggacgc gttccagcgc
      481 tactaccggg cgctcgacga cgaggtcggc gcgttgacgg atctcctgcc ggaggacgtg
      541 atcacgatcg tgatgagcga ccacggggcg cggccgatga tgggaggcat ctgcttcaac
      601 gactggctga tcgagcacgg ctacctcgtg ttgaaggaga aggtcagtgg cgtgacgccc
      661 gtcgcgcgga ccgcgatcga ttggggacgc acgacggcgt ggggtgacgg cggctattac
      721 ggccggttgt tcctgaacgt agccgggcgg gagccgcagg ggtcgatccg accggccgag
      781 tacgagaccg tgcgggacga gctcgtgacg aagctcgagg cgatgccggg tcccgacgga
      841 gcgttgctcg gcaccaaggt acacaagccg caggacgtct acccggaggt caagggtgtc
      901 gcgcccgacc tcctggtgta cttcggggac ctggagtggc gctccgttgg ctccgtgggg
      961 aacccgagca tctacacgtt cgagaacgac atcggaccgg acggcgcgaa ccacgaccgc
     1021 acgggggtgt tcgcgatgaa gggagcgccc gggcagccca gcggcaaggt cgacgggctc
     1081 tcgctcgtcg acgtgggccc caccatcctg tcgctctacg ggatcgattc ccacggagcc
     1141 gcctcgagga gcttcttgtg atgaccacag agcagcacgg attcaccgtc tggttcaccg
     1201 ggctttccgg ctccgggaag tcgacgatcg ccgagatgct cttccacgag ttccaggcac
     1261 gggggctcaa gaccgagatc ctcgacggcg acgtcgtccg gcagaacctc tcgaagggcc
     1321 tcggcttctc caaggaggat cgcgacacga acatcctgcg catcgggttc gtcgcggacc
     1381 tcctcacccg gaacggggtc gcgacgatct gctgcccgat ctcgccctac aaggcggctc
     1441 gagatgccgt gcgcgcccag atcggggacg cgttcgtcga ggtctacgtg cacgcgaccg
     1501 tggccgagat cgccgcgaac cgggatccca agggcctgta caagaaggcg ctcgccgggg
     1561 agatcaccgg cttcacgggc gtggacgatc cctacgaagc acccgagagt ccggagctgg
     1621 tcgtggatac gctcgtcgag accccggagc agtcgctgca gaaggtcatg gacaggctgg
     1681 ccgagctggg gcacatcgag gacgccacgc tccaggtgga aggggaccgc gtccactccg
     1741 ggatgaccga cctccggatc accgagtccg ggcacgtcgc cgaggaacac tgagtcacgg
     1801 gcgacggcta gagtccccgt ccatggatat ccctcgctcg ccggatcccg tctccgaacc
     1861 gaaggcctac caggcccacc tgctctcgct cctgggtgcg gatgacccag cgcacgcgca
     1921 ggcggaggcc gcggcgcgat ggcggtcgct gttcacggag gcggcggatc ggcgagacgt
     1981 acgtcccgag ccctcggagt ggtccgtggt cgaatgtctc gggcacgcgg tcgacgcgga
     2041 ggtcgtctcc gccgcgcggt accgatgggt gctcgcgcac gacgaaccgc ccctgatcgg
     2101 ctacgaccag gacctgtggg tggataccct ccacgccgat cgacgcgacg atcccgagga
     2161 gctcctgggg ctcttcgagc cgctccgcgc ggccaatctg gcgctgtggt cgcgttcctc
     2221 cgaggacgat cgggcccggg tgggggtcca cgcggagcgc ggtcgggaga gcttcgacct
     2281 gctgttccgg atgatcgccg gacacgaccg cttccacctg gctcaggctc ggcgagccct
     2341 cgacgccgtc accgagccct gaacggttac gtcggggtcg gctggtacgc tgaccccatg
     2401 cagcgacccg acgcccatcc gggttcccgg atgggcgtct cgcgttccgt acggcatcgg
     2461 cgggggtgat ctggttggag tacgagatcg ggatcgggaa gacggcccgg cgggcctacg
     2521 gcttcgacga ggtggcgatc gtcccctcac ggaggacccg cgaccccgac gacgtggaca
     2581 tcagctggga ggtcg
//
LOCUS       DASUVE010000330         4039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_541432, whole genome shotgun sequence.
ACCESSION   DASUVE010000330 DASUVE010000000
VERSION     DASUVE010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541432"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VF036_07140"
     CDS             <1..422
                     /locus_tag="VF036_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018854136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX6265039.1"
                     /translation="KWVVDLELTTLDAYDAYWIERGWAKEAPIKTQSRIDTPGSGAEV
                     APGPVPVAGVAWAQHRGIDRVEVRVDDGAWEPAELGAEDTIDTWRQWVYRWDATPGSH
                     ALAVRATDGEGETQTEDTVPPFPDGATGYHSIRVDVI"
     gene            585..3785
                     /locus_tag="VF036_07145"
     CDS             585..3785
                     /locus_tag="VF036_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX6265040.1"
                     /translation="MMRRIIASSLKFRRLVVALAAGLVVFGVVQLRHVPVDQLPEFGP
                     TMVEVRTEALGLSATEVEQLITVPLEQDLLNGVAFVDEIHSESLPGLSSVVMIFEPGT
                     PLLDARQVVQEKVAEAAVALPGVSAPPQMLQPYSSTSRVLTVRLASDTLTPIAISELA
                     KWNIAPRLLGVQGVANVSIWGFRDRQLQVLVDPERLRDADVTLQSVVRTTGNSLWVSP
                     LTFLEASTPGTAGFIDTPNQRIGIRHEPPIRTADDLAQVPVEDDEGNAVIVDGAPVQL
                     GDVTDLVEDHQPLIGDAIFANGETGLMLVVEKFPDANTGEVTDGVEEALDSLRPGLSG
                     LEVDTALFRPADYVDTSARNVAVALAIGGLLLIAGLALLLFEWRSIVIAAAAIISSFA
                     AAGAILILRDVTLNVMIIAGLVMALGIVIDDAVNDVREIASRLKGGGEDGAGASLPAW
                     RSVLEASVAVRSSLLFGTFVAVAAVVPGFFLDRQAGAFLPPILISYLAVIGVSMLVAL
                     VVTPSLGLMLLPGRSEMRESPVLRWFRGRHGSLLTRGVRRPRWAYAAIGVALVIGVAM
                     FPFLDRSANVRLRETDLLIRWDAAPGTSLPAMNGLTADVVDRLAALEGVEQVGAHVGR
                     AIHSDQIVNINSGEIWLTLDDSADHDAAVASIEQVLATYPDLSHRVTTYAEQRITDVL
                     EGRDRDVVVRVYGANRATLETKADEIRGALSGIEGIERTQVEVAPTEPTIEVEVDLLR
                     AQQFGVKPGDVRRASAILLSGLAVGNLFEEQKVFDVVVWGTPDIRRSVEDVEQLLIDT
                     PTGERVRLHEVADVRVVENPAVIRHEAVSNYIDVSADIAGGDTDGVLEDVRSAVAAVE
                     FPLEHHAEIRGAAVNVVDSGATVLAVSLTAALAIFLLLQAAFTSWRLAILVLATLPIA
                     LVGGLVAAAIGGGTIGLGAFAGLLAVLGMTARSGIQMVRHYQHLELEEGETFGDELVL
                     RGTEERLQQSVVPVIVTILALVPIIVAGDVAGLEIARPMAVVMIGGFLTSALLTLFVL
                     PPLYRMHGFVTERDTVAEDLVVLPEPELDVEPVAGS"
     gene            3797..>4039
                     /locus_tag="VF036_07150"
     CDS             3797..>4039
                     /locus_tag="VF036_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265041.1"
                     /translation="MHRRRWTVAIPICAILFLPGCRDLAAEEVEVGYEPATVEEIKGS
                     DLSRVVLEEDAARRIGLDTVPTTGRDGRTVVPDSAVW"
BASE COUNT          583 a         1359 c         1433 g          664 t
ORIGIN      
        1 tgaagtgggt cgtggacctc gagctcacga cgctcgatgc ctacgacgcg tactggatcg
       61 agcgggggtg ggccaaagag gcgccgatca agacgcagtc gcggatcgac acgccgggct
      121 ccggagcaga ggtcgcgccc ggccccgtcc cggtggcggg cgtggcatgg gcgcagcacc
      181 gcgggatcga tcgcgtcgag gtgcgggtcg acgacggagc gtgggagccg gccgagctcg
      241 gcgcggagga cacgatcgac acgtggcggc agtgggtcta tcgatgggat gcgacgccgg
      301 gttcccacgc gctggcggtg cgcgccaccg acggggaggg cgagacccag acggaggaca
      361 cggtcccgcc gttcccggac ggcgcgaccg ggtaccactc gatccgcgtc gacgtgatct
      421 gacctatccc cgaggtagcc cttctaggta tcggcgctac gtcagacgac gcatgtggag
      481 ttgctacagg tagccatatc tttcgacctg tcgcgcaacg gggggcgggc gagcggcccg
      541 gaacggtcca ccgcgaaccc accgcgagag gaggggtccc gtcgatgatg cgcaggatca
      601 ttgcatcgag cctgaagttc cgccgtctcg tggtcgcgct cgcggccggc ctggtcgtct
      661 tcggtgttgt ccagctgcgc cacgtcccgg tcgatcaact gcctgagttc ggccccacga
      721 tggtggaggt ccggacggaa gcgctcggtc tctcggcgac cgaggtcgag cagctgatca
      781 ccgtgccgct cgagcaggac ctgctgaacg gcgtcgcatt cgtcgacgag atccactcgg
      841 aatccctgcc ggggctctcc tcggtcgtca tgatcttcga gcccggtacg cccctcctcg
      901 atgcgcgaca ggtggtacag gagaaggtcg ccgaggccgc cgtcgcgctc cccggggtgt
      961 ccgcaccccc gcagatgctg cagccgtatt cctccacgag ccgggtcctc accgtccggc
     1021 tggcctccga caccctgaca ccgatcgcga tctcggagct cgccaagtgg aacatcgcgc
     1081 cacgtttgct cggggtccag ggcgtggcca acgtctcgat ctgggggttc cgggatcgcc
     1141 agctgcaggt gctggtggac ccggagcgcc tgcgggacgc ggacgtcacc ctccagtcgg
     1201 tcgtgcgcac gacggggaac tccctgtggg tctccccact gaccttcttg gaggcctcca
     1261 cccccggaac cgcgggattc atcgacacgc cgaaccagcg gatcgggatc cgtcacgagc
     1321 ctccgatcag gacggcggac gatctggcgc aggtcccggt cgaggacgac gagggcaacg
     1381 cggtcatcgt cgatggggca ccggtccagc tcggcgacgt gacggacctc gtcgaggacc
     1441 accagccgct gatcggcgac gcgatcttcg ccaacggcga gaccgggttg atgctggtcg
     1501 tcgagaagtt cccggacgcg aacaccggtg aggtgacgga cggtgtggag gaggcgctgg
     1561 actcgctccg accggggctg tccgggctcg aggtcgacac cgccctcttc cgcccggctg
     1621 actacgtcga cacatccgca cggaacgtcg ccgtggcgct cgcgatcggc gggctcctcc
     1681 tgatcgcggg gctggccctg cttctgttcg agtggcgctc gatcgtgatc gccgccgccg
     1741 cgatcatctc ttcgttcgcg gccgcgggcg cgatcctcat cctccgcgac gtcacgctga
     1801 acgtgatgat catcgccggc ttggtgatgg cgctgggcat cgtgatcgac gacgccgtca
     1861 atgacgttcg cgagatcgcc tcgcggctca agggcggcgg cgaggacggg gccggagcca
     1921 gcctgcctgc ctggcgatcg gtcctcgagg cctccgtcgc cgtgcggagc tccctcctgt
     1981 tcgggacctt cgtcgctgtc gccgccgtgg tccccggctt cttcctcgac agacaagcgg
     2041 gagcgttcct gccccccatc ctgatctcgt acctggccgt gatcggtgtc tcgatgctgg
     2101 tcgcgctggt cgtcacgccc agcctcggct tgatgctgct gcccggccgt tcggagatgc
     2161 gcgagtcccc ggtgctccgc tggttccggg gccgacacgg gtcgctgctc actcggggtg
     2221 tccgcagacc gcgatgggcc tacgcggcga tcggggtggc gctcgtcatc ggcgtcgcga
     2281 tgttcccgtt cctggaccgc tcggcgaacg ttcggctgcg cgagaccgac cttctgatcc
     2341 gatgggacgc cgctccgggc acgtcgttgc cggcgatgaa cgggttgacc gcggacgtcg
     2401 tcgaccggct ggccgcgctc gagggcgtcg agcaggtggg cgcccacgtc ggccgcgcga
     2461 tccactccga ccagatcgtg aacatcaaca gcggagagat ctggctgacc ctcgatgact
     2521 cggcggacca cgatgccgcg gtcgcgtcga tcgagcaggt cctggcgacc tacccggacc
     2581 tctcgcaccg cgtgaccacg tacgcggagc agcggatcac cgatgtgctc gaaggccgcg
     2641 accgagatgt cgtcgtgcgc gtgtacggcg cgaacagagc caccctcgag acgaaggccg
     2701 acgagatccg cggagcgttg tccgggatcg aaggcatcga acggacacag gtcgaggtgg
     2761 cccccaccga gccgacgatc gaggtcgaag tggatctcct gcgcgcgcag cagttcggcg
     2821 tgaagcccgg agacgtccgc cgagcctcgg cgatcctgct ctccgggctc gccgttggca
     2881 acctgttcga ggagcagaag gtgttcgacg tggtcgtgtg gggaacgccc gacatccgcc
     2941 ggtcggtcga ggacgtcgag cagctgctga tcgatacgcc gaccggcgag cgggtccgcc
     3001 tccatgaggt ggccgacgtc cgcgtcgtcg agaaccccgc cgtgatccgt cacgaagcgg
     3061 tgtccaacta catcgacgtc agtgccgaca tcgcgggcgg tgacaccgac ggggtgctgg
     3121 aggacgtccg gagcgcggtc gcggctgtgg agttccccct cgagcaccac gcggagatca
     3181 ggggcgccgc cgtgaacgtc gtggattcgg gggccacggt cctcgccgtc tcgctcacgg
     3241 ctgccctcgc gatcttcctc ctcctccagg ccgcgttcac cagctggcgg ctcgcgatct
     3301 tggtcctcgc gacccttccc atcgcgctcg tcggcgggct cgtggccgcc gcgatcggtg
     3361 gggggaccat cggcctcggc gccttcgcgg gtctcctcgc ggtcctgggg atgaccgcac
     3421 gcagcggcat ccagatggtg cggcactacc agcacctcga gctcgaggag ggcgagacgt
     3481 tcggcgacga gttggtgctc cgcggcaccg aggagcgcct gcaacagtcg gtcgtgccgg
     3541 tcatcgtcac gatcctcgcg ctggtgccga tcatcgtggc cggcgacgtc gccgggctcg
     3601 agatcgcgag gccgatggcg gtcgtgatga tcggcgggtt cctcacctcc gcgctcctca
     3661 cgctgttcgt gttgccaccg ctctaccgga tgcacgggtt cgtcaccgag cgtgacaccg
     3721 tcgcggaaga cctggtggtc ctgccggagc cggagctcga cgtcgaacca gtggccggga
     3781 gctgagggga ggatcgatgc atcgtcgtcg atggacggtc gcgatcccga tctgcgcgat
     3841 cctcttcctc cccggttgcc gggacctcgc ggcggaggaa gtcgaggtgg ggtacgaacc
     3901 cgcgaccgtc gaggagatca agggatcgga tctgtcccgg gtggtcctcg aggaggacgc
     3961 ggcccggcgg atcgggctcg acacggtccc gacgacgggc cgtgacggtc ggacggtggt
     4021 gccggactct gcggtctgg
//
LOCUS       DASUVE010000331         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_162776, whole genome shotgun sequence.
ACCESSION   DASUVE010000331 DASUVE010000000
VERSION     DASUVE010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162776"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..700
                     /locus_tag="VF036_07155"
     CDS             5..700
                     /locus_tag="VF036_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX6265042.1"
                     /translation="MTGGSFSIGALLTSDSIGDATDRLQYTQSVVQGDADLATKVAVT
                     AEEIRRQETRLRQSARIEAIASEALAEQRADLLLRIEDIDTLIADLEAELDAQQEATL
                     GLGGGVVFRSGAIQTCPAVGTHSFVDSFGDPRPGGRSHAGIDIMAAPGTPVVAVAPGN
                     ARPAGSIGGLGAVVEHPNGDWTFYAHLSSYGTLGSVSTGTVIGYVGDHLHFEYHPNGG
                     AAVNPYGMLLAVC"
     gene            729..1259
                     /locus_tag="VF036_07160"
     CDS             729..1259
                     /locus_tag="VF036_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aerial mycelium formation protein"
                     /protein_id="HEX6265043.1"
                     /translation="MDEEHPGNRRIDRIREPSYLEGVDSKSLEDVRALRDECMAEREY
                     LSLLRRLVQGRAEILKAELASRGSDDHRPLVDRLSEILASDEPATSRGEAMHVSLPEE
                     EMLLARRRIERLVADAGISDPTELDDPKLEEAIELLAGEEREVSAQRTDVLRVLDAVQ
                     DELKRRYKEDPSLALS"
     gene            1396..2175
                     /locus_tag="VF036_07165"
     CDS             1396..2175
                     /locus_tag="VF036_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX6265044.1"
                     /translation="MKLERGGSEAATRDPIGDSEAGSLHDQLKVFAREVGVLYSAERV
                     RRRELEHALESAREMYVATMKSLAHVVEAKDPTTRGHLDRTAHYGLALARKVDPELAE
                     RPEVAYGFFLHDIGKVGIPESILCKTGPLTELEWVVMRSHPNQGARIVEPIPFMGEAV
                     EIVRCHHERFDGEGYPMGLRGEDIPLAARIFSIADSFDAMTSDRPYRSALSLDEAVAE
                     IRAGAGTQFDPRCVEAFEELAAEEDVLVLPGKRRTPSFATG"
     gene            2217..>2428
                     /locus_tag="VF036_07170"
     CDS             2217..>2428
                     /locus_tag="VF036_07170"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018962925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX6265045.1"
                     /translation="MTAPAELVHVVRNGLVESVHTGDVAVCDADGRLRAAAGDPQRLL
                     FGRSCEKPLQGAVSFSAMDEPDLPDD"
BASE COUNT          391 a          852 c          821 g          364 t
ORIGIN      
        1 gttcatgacg ggcgggtcgt tctcgatcgg cgctctcttg acgtcggact ccatcgggga
       61 cgccaccgac cgactgcagt acacgcagag cgtcgtccaa ggcgacgccg acctcgcgac
      121 gaaggtcgcc gtcaccgccg aggagatccg tcgccaggaa acccgcctcc gccagtccgc
      181 tcggatcgag gccatcgcgt cggaagcgct cgcggagcag cgggcagacc tgctcctccg
      241 gatcgaggac atcgacacgc tgatcgccga cctcgaagcg gagctcgacg cgcagcagga
      301 ggccacgctc ggattgggag gtggtgtcgt cttccggtcc ggcgcgatcc agacgtgccc
      361 cgcggtcggc acgcactcgt tcgtcgactc cttcggcgac ccgaggccgg gcggtcggtc
      421 gcacgcgggc atcgacatca tggcggcgcc gggcacaccg gtcgtcgccg tcgcccccgg
      481 gaacgcccga ccggcgggga gcatcggcgg cctgggcgct gtcgtcgagc acccgaacgg
      541 cgactggacc ttctatgccc acctgtcttc gtatggcacc ctcggttcgg tgtctacggg
      601 aaccgtgatc ggatacgtcg gcgatcacct gcacttcgag taccacccga acggcggggc
      661 ggccgtgaat ccgtacggga tgctcctcgc cgtctgctga gcacgggggc cgggtgtagc
      721 atcgcctcgt ggacgaggag cacccgggta accgccgtat cgatcggatc cgcgagccct
      781 cgtacctgga gggggtcgat tccaagtcgc tcgaggacgt gcgggcgctc cgcgacgaat
      841 gcatggccga acgcgaatac ctctccttgc tccgcaggct cgtccagggc cgcgcagaga
      901 tcctgaaggc cgagcttgcc tcccggggga gcgacgacca tcggccgctc gtggatcgac
      961 tctcggagat cctcgcgtcc gatgagcccg ccacgtcccg cggcgaggcg atgcacgtct
     1021 ccctgcccga ggaggagatg ctcctcgccc gccgccggat cgagcgtctg gttgctgatg
     1081 caggcatcag cgacccgacg gaacttgacg atcccaagct cgaggaggcg atcgagctgc
     1141 tcgcgggcga ggaacgcgaa gtttcggctc aacgaaccga cgtgctccgt gtgctcgacg
     1201 cggttcagga cgagctgaag cggcgataca aggaagatcc gagcctggcg ctcagctgag
     1261 gttccgcggc gtgccgctgg cgtcccccgg tcgccggcca cgaacccccc ggtcgcccga
     1321 tacccccgct cccggagccc gtgcgaacgt gtgccccgtt ctccacaaca ggggataagc
     1381 gaggaccggg cgaacatgaa actcgaacga gggggatccg aggccgccac cagggatccg
     1441 atcggggatt cagaggcggg ctcgctgcac gaccagctca aggtgttcgc ccgtgaggtc
     1501 ggcgtcctgt attcggcgga acgtgtccgc cgacgcgagc tagagcacgc gctcgagagc
     1561 gcccgcgaga tgtacgtggc cacgatgaag tccctcgccc acgtggtgga agccaaagac
     1621 ccaacgacgc ggggacacct cgatcgcacg gcgcactacg gtctcgcgct cgcacggaag
     1681 gtcgaccccg agctcgcgga gcgaccggag gtcgcctacg ggttcttcct ccacgacatc
     1741 gggaaggtcg gcatccccga gtcgatcctc tgcaagaccg gcccgctcac cgagctcgaa
     1801 tgggtcgtga tgcgatccca tccgaaccag ggcgcacgga tcgtcgaacc gatccccttc
     1861 atgggagagg ccgtcgagat cgtccggtgt catcacgagc gcttcgacgg ggagggatat
     1921 ccgatgggac tccgcggaga ggacatcccc ctggccgccc gcatcttctc gatcgcggac
     1981 tcgttcgacg cgatgacgag cgaccggccc taccgttcgg cgctctccct cgacgaggcc
     2041 gtcgcggaga tccgcgccgg tgcgggcacc cagttcgacc cgcgctgtgt ggaggcgttc
     2101 gaggagctcg ccgcggagga ggacgtcctc gtcctccccg gcaagcgccg gacgccatcc
     2161 ttcgccacgg gctgagtccg gccgcccctg acgggcgtcg gtaggcttcc gccccgatga
     2221 ccgctcccgc cgagctcgtc cacgtcgtcc gcaacggcct cgtcgaatcg gtgcacacgg
     2281 gcgatgtcgc cgtctgcgac gcggacggca ggctgcgcgc cgcggccggc gacccccaac
     2341 gtctcctctt cggtcgctcc tgcgagaagc cgctgcaggg ggccgtctcg ttctcggcga
     2401 tggacgagcc ggatcttccg gacgacga
//
LOCUS       DASUVE010000332         6082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_352560, whole genome shotgun sequence.
ACCESSION   DASUVE010000332 DASUVE010000000
VERSION     DASUVE010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352560"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="VF036_07175"
     CDS             complement(<1..1123)
                     /locus_tag="VF036_07175"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX6265046.1"
                     /translation="MAAERGQGAAVVSDPDVVRFFDTTLRDGEQAPGIALSKDEKVEI
                     AEQLARLQVDILEAGFAISSDGEFDAVREIATSVTGPVIASLARVVPGDIERAAEALK
                     GADRFRIHVFISTSQIHMVDMLRMSHRQVLGGIAEGVKLAAGYTDDVEFSAQDATRTE
                     LDFLLECFDVAVKAGATTINVPDTVGYATPAEFGELVRIVRERTPEHVVISTHCHNDL
                     GLAVANSLAGVENGARQVEVAVNGIGERAGNCSLEEMAMIVRTRSEALGVRHGLNLKE
                     IVRTSRLVSALTGYSVQPNKAVVGASAFAHESGIHQHGVLANRATYEIMDPVEIGLEG
                     NQIVLGKHSGRHAFADALDKVGIAIDEDHMHRAFVRFKEL"
     gene            complement(1087..2100)
                     /gene="ilvC"
                     /locus_tag="VF036_07180"
     CDS             complement(1087..2100)
                     /gene="ilvC"
                     /locus_tag="VF036_07180"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX6265047.1"
                     /translation="MATIYHEQDTDVSALAGQRIAILGFGSQGHAHAQTLKDSGHDVR
                     VGLRPGSTSRTTAEEAGLRVVDTADAVEEADVAMVLLPDTSHGKVYAEEIAPHLNAGD
                     MLMFAHGFSIHFGTVTPPPDVDVTMIAPKGPGHLVRRTYEEGIGTPALVAVQQDATGK
                     ARQRALAYGHGIGAARAGIIETTFKEETETDLFGEQTVLCGGLSELIRSGFDTLVAAG
                     YQPEIAYFECLHEVKLIVDLIYEGGLSWMRHSISDTAEWGDYVSGPRVIDDGTREAMR
                     QLLGEIQDGSFAKRWIAEADGGFPEFQAKRREARASQLEDVGRQLRPMMPWLQSEDKV
                     PPS"
     gene            complement(2109..2672)
                     /gene="ilvN"
                     /locus_tag="VF036_07185"
     CDS             complement(2109..2672)
                     /gene="ilvN"
                     /locus_tag="VF036_07185"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX6265048.1"
                     /translation="MSLHTISVLVEDKPGVLTRVAGLFSARGYNIDSLAVGPTEAEGL
                     SRMTIVVNVESKSLEQVTKQLNKLINVIKILEHEPGTAVERELMLAKVRAKGDARARI
                     MEIAEVFRVSIVDVTKTTLTVEASGKPEKLEALRELLSEFGIVELSRTGRIALARGDR
                     GIREKSGLRVAKVVGAEEASEWTGGGV"
     gene            complement(2669..4414)
                     /locus_tag="VF036_07190"
     CDS             complement(2669..4414)
                     /locus_tag="VF036_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX6265049.1"
                     /translation="MKVSGAQALFKALEGEGVDIAFGIPGGAILPAYDPLIESGVRHV
                     LCRHEQGAGHAAEGYAWATGKVGVAIATSGPGGCNLVTALADAKMDSVPIVAITGQVP
                     MPAVGNDAFQEADITGITMPVTKHNFLVTDPGEVAETIREAFHIARTGRPGPVLVDLP
                     KDVLVNETTWSWPDQVDLPGYRPTTKGNMRQVVEAAKLILEAKRPVLYVGGGVIKAEA
                     TDELYELATVGHLPVVTTLMARGAFPDAHELCLGMPGMHGVYTAVTAMQKADLLVAVG
                     ARFDDRVTGKLATFAPGAKIVHIDIDPAEIGKNRKADVPIVGDCKDVTAKLVAELTRR
                     QDEAGIPDRSPWLEQLATWKAEQPLRYDQEPDGPLKPQYAIERLRAHSDGDTIVVAGV
                     GQHQMWASQFWSFEKPRTWINSGGLGTMGFAVPAALGAAAGQPDRRVIAIDGDGCFQM
                     TSQELATSTTEKIPFITAIINNAYLGMVRQWQELFYEERYSQVYLGYDVPDYVKLAEA
                     YGAVGLRAERPEEVDAVIEKALSVDDRSVVIDFRCDANEMCFPMVPAGASNDDIITGP
                     EGLQPQDDPAEAPIA"
     gene            complement(4411..4707)
                     /locus_tag="VF036_07195"
     CDS             complement(4411..4707)
                     /locus_tag="VF036_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265050.1"
                     /translation="MRYEFEKDGATVASVLWEGPGQVSVETDDPSTRTTVERYLSSEV
                     TYLSGFGGEELQSRRRDWTPWEFERACRNLARRLGATVKRVETGPVEERQAVAG"
     gene            4956..5186
                     /locus_tag="VF036_07200"
     CDS             4956..5186
                     /locus_tag="VF036_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265051.1"
                     /translation="MDPLPDEGSPIPRVHPLEIPFVWFGLQLIEYAIVWLLITTLLPD
                     SMPGPVGWALLLVLMVGLTILNYMIRRRFIPR"
     gene            5254..>6082
                     /locus_tag="VF036_07205"
     CDS             5254..>6082
                     /locus_tag="VF036_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase family protein"
                     /protein_id="HEX6265052.1"
                     /translation="MVTDASTDPALDAAIRAIPSWDGAELEIIPIAEGRTNRNFRVTA
                     DDEAFFLRLSEKSTELLGIDRTAEQDSVRAAAEAGVGSEVFAYLPDLGCLVTRWIDAD
                     PLSEGDLEREEVLRPVVDAIKAIHAGPYLPSSFDPFRIVEDYRRISEERGVIVPDAFD
                     GAHEKASRIEAAFASDPMPLLPCHNDLLEANFLLRDGHVWVVDHEYAGMGDPFFDLGN
                     LSINNALSDDAQERLLSLYFEEPNDGHRARLKLMRIMSDFREAMWGVVQQGLSTLDID
                     "
BASE COUNT          849 a         2179 c         2012 g         1042 t
ORIGIN      
        1 cgagctcctt gaagcgcacg aacgcgcgat gcatgtggtc ctcgtcgatc gcgatgccca
       61 ccttgtcgag cgcgtcggcg aacgcgtggc gcccggagtg cttcccgagg acgatctggt
      121 tgccctccag gccgatctcg accggatcca tgatctcgta cgtggcgcgg ttcgcgagca
      181 caccgtgctg gtggatgccg ctctcgtgcg cgaacgcgct cgcccccacg accgccttgt
      241 tcggctgtac cgagtacccg gtgagggccg agacgagccg cgacgtccgc acgatctcct
      301 tcagattcag gccgtgccgc acaccgagcg cctcgctgcg ggtccggacg atcatcgcca
      361 tctcctcgag cgagcagttg ccggcgcgct cgccgatccc gttcaccgcg acctccacct
      421 ggcgcgcacc gttctccacc ccggccagcg agttcgccac ggcgaggccg agatcgttgt
      481 gacagtgcgt ggagatcacg acgtgctcgg gcgtccgctc ccgcacgatc cgcacgagct
      541 cgccgaactc cgccggggtt gcgtagccga ccgtgtcggg gacgttgatc gtcgtcgcgc
      601 cggccttcac ggcaacgtcg aagcactcga ggaggaagtc cagctccgtc cgggtggcgt
      661 cctgcgcgct gaactccacg tcgtcggtgt agccggccgc gagcttcacg ccttcggcga
      721 tcccgccgag gacctgccgg tgggacatcc ggagcatgtc caccatgtgg atctgcgacg
      781 tcgagatgaa gacgtggatc cggaaccgat cggcgccctt cagcgcctcg gcggcgcgct
      841 cgatgtcgcc ggggaccacg cgggcgagcg acgcgatcac cgggcccgtc acgctcgtcg
      901 cgatctcgcg caccgcgtcg aactcaccgt cggaggagat cgcgaacccc gcctcgagga
      961 tgtcgacctg cagccgggcg agctgctccg cgatctcgac cttctcgtcc ttggacagcg
     1021 cgatgccggg ggcctgctcc ccgtcgcgca gggtggtgtc gaagaaccgg actacgtcgg
     1081 ggtcgctcac gacggcggca ccttgtcctc gctctgcagc cacggcatca tcggccgcag
     1141 ctgccggccg acgtcctcga gctgggacgc tcgcgcctcg cgccgcttgg cctggaactc
     1201 agggaacccg ccgtcggcct cggcgatcca gcgcttcgcg aacgatccgt cctggatctc
     1261 cccgaggagc tgtcgcatcg cctcgcgcgt gccgtcgtcg atcacgcgcg ggccggagac
     1321 gtagtcaccc cactcggcgg tgtctgagat ggagtggcgc atccaggaga gacccccctc
     1381 gtagatcagg tcgacgatca gcttcacctc gtggaggcac tcgaagtagg cgatctccgg
     1441 ctggtagccg gccgccacga gcgtgtcgaa cccggagcgg atcagctcgg acagtccgcc
     1501 gcagagcacg gtttgctcgc cgaacaggtc ggtctcggtc tcttccttga acgtggtctc
     1561 gatgatgccc gcacgagcgg cgccgatccc gtggccgtac gcgagcgctc gctggcgggc
     1621 cttcccggtg gcgtcctgct ggaccgcgac gagcgcggga gtcccgatgc cctcttcgta
     1681 ggtgcgccgc acgaggtggc cgggcccttt cggcgcgatc atcgtgacgt cgacgtcggg
     1741 cgggggggtg accgtcccga aatggatcga gaagccgtgc gcgaacatca gcatgtcgcc
     1801 ggcgttcagg tggggggcga tctcctccgc gtacaccttc ccgtgcgagg tgtcggggag
     1861 caggaccatc gcgacgtcgg cctcctcgac cgcgtccgcc gtgtcgacca cccggaggcc
     1921 ggcctcctcg gccgtggtgc ggctcgtgga gccgggtcgc aggcccacgc gcacgtcgtg
     1981 tcccgagtcc ttcagggtct gggcgtgcgc gtggccctgc gacccgaacc cgaggatcgc
     2041 gatcctctgc cccgccagcg cggatacgtc ggtgtcctgc tcgtggtaga tcgtcgccat
     2101 cgttccccct agaccccgcc gccggtccac tccgatgctt cctccgcgcc cacgaccttc
     2161 gcgacccgca gaccgctctt ctccctgatc ccacggtcgc cgcgcgccag cgcgatccgt
     2221 ccggtccgcg agagctccac gatcccgaac tcggacagca gctcgcggag cgcctcgagc
     2281 ttctccggct tgccggacgc ctcgaccgtg agcgtcgtct tcgtcacgtc gacgatgctc
     2341 acgcgaaaca cctctgcgat ctccatgatc ctcgcgcgcg cgtcaccctt cgcccgcacc
     2401 ttggcgagca tcagctcccg ctcgacggcc gtgccgggct cgtgctcgag gatcttgatc
     2461 acgttgatca gcttgttcag ctgcttcgtc acctgctcca gggacttcga ctcgacgttc
     2521 acgacgatcg tcatccggga gaggccctcg gcctccgtgg ggccgaccgc gagcgagtcg
     2581 atgttgtagc cgcgcgccga gaacagaccg gcgacgcgcg tcaggacgcc gggcttgtcc
     2641 tcgacgagga ccgagatcgt gtgcaggctc atgcgatcgg tgcctcggct ggatcgtcct
     2701 gcggctgcag accttcgggt ccagtgatga tgtcgtcgtt cgacgcgccc gcggggacca
     2761 tcgggaagca catctcgttc gcgtcgcagc ggaagtcgat caccacgctc cggtcgtcga
     2821 ccgacagcgc cttctcgatc accgcgtcga cctcctcggg ccgctcggcg cgcagaccga
     2881 ccgcgccgta ggcctcggcg agcttcacgt agtcgggcac gtcgtagccc aggtacacct
     2941 gcgagtagcg ctcctcgtag aagagctcct gccattgccg caccatgccc aggtaggcgt
     3001 tgttgatgat cgcggtgatg aacgggatct tctccgtcgt cgaggtggcg agctcctgcg
     3061 aggtcatctg gaagcagccg tcgccgtcga tcgcgatcac gcgccggtcg ggctgaccag
     3121 ccgccgcgcc gagggccgcc ggcaccgcga aacccatcgt gccgagcccg ccggagttga
     3181 tccacgtgcg cggcttctcg aaggaccaga actgcgaggc ccacatctgg tgctggccca
     3241 cgccggccac gacgatcgtg tcgccgtcgc tatgcgcacg gagccgctcg atcgcgtact
     3301 gcggcttgag cggcccgtcc ggctcctggt cgtagcggag cggctgctcg gccttccacg
     3361 tcgcgagctg ctcgagccac gggctcctgt ccgggatgcc cgcctcgtcc tgacggcgcg
     3421 tgagctccgc gacgagcttc gccgtcacgt ccttgcagtc ccccacgatc gggacgtcgg
     3481 ccttgcggtt cttgccgatc tccgcgggat cgatgtcgat gtgcacgatc ttcgcgccgg
     3541 gcgcgaacgt ggcgagcttg ccggtcacac ggtcgtcgaa gcgagccccg acggcgacga
     3601 gcaggtccgc cttctgcatc gcggtcaccg ccgtgtagac gccgtgcatg cccggcatcc
     3661 cgaggcacag ctcgtgggcg tcggggaacg cgccgcgcgc catcagcgtc gtgacgaccg
     3721 gcaggtgtcc cacggtcgcg agctcgtaca gctcgtcggt ggcctccgcc ttgatcacac
     3781 cgccgccgac gtagaggacc ggtcgcttcg cctcgaggat cagcttcgcg gcctccacca
     3841 cctggcgcat gttgcccttg gtcgtgggcc ggtaccccgg gaggtcgacc tggtccggcc
     3901 agctccacgt ggtctcgttc accaggacgt ccttcgggag gtcgacgagg accgggccgg
     3961 gccggccggt acgcgcgatg tggaacgcct cgcggatcgt ctcggcgacc tcgccgggat
     4021 cggtgacgag gaagttgtgc ttcgtgaccg gcatcgtgat gccggtgatg tccgcctcct
     4081 ggaaggcgtc gttgccgacg gccggcatcg ggacctgtcc ggtgatcgcg acgatgggga
     4141 cggagtccat cttcgcgtcg gcgagcgcgg tcacgaggtt gcagccgccc gggcccgacg
     4201 tggcgatcgc gacgccgacc ttcccggtgg cccaggcgta gccctccgcg gcgtgccctg
     4261 ctccctgctc gtgccggcac agcacgtggc ggacacccga ctcgatcagc gggtcgtagg
     4321 ccggcaggat cgcgccgccc gggatcccga aggcgatgtc gaccccttcg ccctcgaggg
     4381 ccttgaacag cgcctgggcg ccgctgacct tcatcccgcg accgcctgcc gctcctcgac
     4441 ggggccggtc tcgacccgct tcaccgtggc gcccaggcgg cgggccaggt tgcggcaggc
     4501 acgctcgaac tcccacgggg tccagtcgcg gcgcctgctc tgcagctcct ctccgccgaa
     4561 gccgctcagg taggtgacct cggaggacag gtagcgctcc acggtcgtcc tcgtgctcgg
     4621 atcgtcggtc tcgacgctca cctggcccgg tccttcccac agcacgctgg cgacggtggc
     4681 gccgtccttc tcgaactcgt agcgcatcgg atgctcctct ctgtttccgt gtttcggctg
     4741 gtcgtgggca gaaaaaaacc ctcgcgtggc gagggtgtcg gcgcgggacg cgggctctca
     4801 ggcccggccg cgcctagcga ataagtacga ggacggtgct cggcgtgatc ctcgcgctct
     4861 tcatggtcgg gccagtgtcc gcgctcggcc gaggagtgtc aacccccgga cggccgatgg
     4921 catctgtgct gcggggacca gatacgatga cgcccatgga cccgctcccc gacgaggggt
     4981 cgcccatccc ccgcgtccat ccgctcgaga tcccgttcgt gtggttcggg ctccagttga
     5041 tcgagtacgc gatcgtgtgg ctgctcatca cgacgttgct ccccgactcg atgcccggtc
     5101 cggtcggctg ggcactcctc ctcgtgctca tggtcggcct cacgatcctc aactacatga
     5161 tccgacgcag gttcatccct cggtagagca cctcgaggac caaaccgcgg ctcgttcgac
     5221 caccgacgag gccggtgcta gcgttcggcc gccatggtca cggacgcctc caccgatccc
     5281 gcgctggacg ccgcgatccg cgcgatcccg tcgtgggatg gcgcggagct cgagatcatc
     5341 ccgatcgccg agggtcgcac caatcggaac ttccgcgtca cggccgacga cgaagccttc
     5401 ttcctccgtc tctccgagaa gtccaccgag ctgctcggga tcgatcggac cgcggagcag
     5461 gactctgtcc gagcggccgc ggaggcgggc gtggggtcgg aggtgttcgc gtatctccca
     5521 gatctcggct gcctcgtgac ccgctggatc gacgccgatc ccttgtccga gggcgatctc
     5581 gagcgcgagg aggtgctccg tcccgtggtc gatgcgatca aggcgatcca cgccggaccg
     5641 tacctcccct cgtcgttcga cccgttccgg atcgtggagg attaccggcg catctccgag
     5701 gagcgcggcg tgatcgtgcc cgacgccttc gacggggcgc acgagaaggc gtcccggatc
     5761 gaggccgcgt tcgcgagcga tccgatgccc ctcctcccct gccacaatga cctgctcgag
     5821 gcgaacttcc tcctgcgcga cggccacgtc tgggtggtgg accacgagta cgcggggatg
     5881 ggcgacccgt tcttcgacct cggcaacctc tcgatcaaca acgcgctgtc ggacgacgcg
     5941 caggagcgac tcctctccct gtacttcgag gagccgaacg atgggcaccg tgctcgcctg
     6001 aagctgatgc ggataatgtc cgacttccgc gaggcgatgt ggggcgtcgt gcagcagggc
     6061 ctgtcgacgc tcgacatcga ct
//
LOCUS       DASUVE010000333         2957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1296251, whole genome shotgun sequence.
ACCESSION   DASUVE010000333 DASUVE010000000
VERSION     DASUVE010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296251"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            598..981
                     /locus_tag="VF036_07210"
     CDS             598..981
                     /locus_tag="VF036_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6265053.1"
                     /translation="MATDLAVSVADQPGGLADVGEALGNAGINIEGLCGVGLGDRGVI
                     HILVEDGAAARAALEGAGLNVESEAEAIVSAIPGDVRAPGTLGKMARAVAEAGVNMRA
                     VYLATDNRAVAVTDDNAKALVALGM"
     gene            1026..1412
                     /locus_tag="VF036_07215"
     CDS             1026..1412
                     /locus_tag="VF036_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6265054.1"
                     /translation="MATDLTVILDDRAGQLAHMGEALGDAGVTIEGISATTSDGIGIV
                     HVLVDNAMVAQNALILADIKVEGEADAVVIDLTAEEAGKPGTLGRLAGKVSMAGINVS
                     VAYLATRDRVALVTNDNERAREALQA"
     gene            complement(1450..>2957)
                     /gene="glgX"
                     /locus_tag="VF036_07220"
     CDS             complement(1450..>2957)
                     /gene="glgX"
                     /locus_tag="VF036_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013153839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX6265055.1"
                     /translation="LLPVHQFVDDRHLVERGLGNYWGYNSIGFFAPHHAYATRSVGQQ
                     VYEFKSMVKTLHRAGIEVILDVVYNHTAEGNHLGPTLSLRGIDNKAFYRLSPDDPRYY
                     IDFSGTGNSLNMLHPRAIQLIMDSLRHWVLEMHVDGFRFDLAPVLARELYEVNRLGTF
                     FDIIQQDPVLSQVKLIAEPWDVGPGGYQVGNFPVGWAEWNGKYRDCVRRFWRGDPGQL
                     PELAFRLSGSSDLYEASGRNTYASVNFVTAHDGFTLQDLVTFESKHNEANGEGNRDGS
                     DENFSANWGVEGPSDQPEVVKLRERMKRNLIATLIFSQGVRMLLGGDEIARTQRGNNN
                     AYGQDNEVSWFDWEHRDKAMLNFTRSALQIFRSNPVLRRRSFFSGTFDGGGRKDVMWV
                     RLDGEEMTDEDWADPENRVLGMLIPGRATDERDDRGRRIRGDTVLLLLNGGTRSKKVT
                     LPRFEGGGVWHEILQTANPGQTRVIRREALNLVAHSLMLLRFVGAERSSER"
BASE COUNT          479 a          973 c         1041 g          464 t
ORIGIN      
        1 gccgcccccc gacggcgtcg gctgataacg ccgtcgcggg cgaccggtcg gtcgccgaga
       61 taggaggttc agccctccgg ggttcagctc gagagatcct gctccgcgtc ctcgcgcggc
      121 cacgccgacg gctccggatc gcgcaggaag cggccgagct cctcggcctc ggacccggtg
      181 aggctgatcg ccgccaccgg gcgatcgacg tgccagatcg agatgcgagc gtgatctccg
      241 ggaacggtga tcgttccctc cggtgcggag aactcgtgat cctccaggta cctgctgacc
      301 cggaggacgg gcccaggtgg gtcaccgtcg ctggtctgga tcccgagata gagcctcgtg
      361 atcttggcac ctcgggatcg ctcgtcgccg gccgggagcg gcgccccgca cgaggcgcag
      421 gatccagact cctcgggcgc cccgacgccg catcgcggac agtacgtgca ccgatcctcg
      481 tcccgcggtc gacgatcgca agccctggtg catcgggggc ttgacgtccg cccgccctcg
      541 ggcgtacacc ccctctgaga aggcgagcac gagggggaac gcgagagggg gccagcgatg
      601 gcgacggatc tagcggtcag cgtggcggat caacccggtg ggttggccga cgtgggtgag
      661 gctctcggca acgccgggat caacatcgaa gggctgtgcg gcgtcggtct cggcgatcga
      721 ggggtgatcc atatcctggt cgaagacggg gccgcggccc gcgccgcgct cgagggcgcc
      781 ggtctcaacg tcgagagcga ggccgaggcg atcgtgagcg cgatcccggg ggacgtgagg
      841 gctcccggga cgctggggaa gatggcgcgc gcggtcgcgg aggcgggggt caacatgcgg
      901 gccgtctatc tcgcgaccga caatcgggcc gtcgccgtga cggacgacaa cgcgaaggcg
      961 ctcgtggccc tcgggatgta gtgctccgag aacggaagac gttcggatga agggggggtg
     1021 agcggatggc gacggacctg acggtcatac tggacgatcg cgcgggccag ctcgcacaca
     1081 tgggagaggc gctcggcgac gcgggggtca cgatcgaggg gatcagcgcg acgacgagcg
     1141 acggcatcgg gatcgtgcac gtgctcgtcg acaacgcgat ggtcgcgcag aacgccctga
     1201 tcctcgccga catcaaggtc gagggcgagg ccgacgcggt cgtgatcgac ctgaccgccg
     1261 aggaggcggg caagccaggg acgctgggac ggctcgccgg caaggtatcg atggcgggga
     1321 tcaacgtctc ggtcgcctac ctggcaaccc gtgatcgagt ggcgctcgtg accaacgaca
     1381 acgagcgggc acgggaggcg ctccaggcct aagggccggc cgctccccgg tccctcgtgc
     1441 cctcggggcc taccgctccg aggaccgctc ggcgcccacg aaccgcagca gcatcagcga
     1501 gtgcgcgacc aggttcaatg cctcgcgccg gatcacgcgc gtctgaccgg ggttcgccgt
     1561 ctggaggatc tcgtgccaga cgccgccccc ttcgaagcgg gggagggtga ccttcttcga
     1621 gcgggtccca ccgttcaaca ggaggaggac ggtgtcgccg cggatccgtc gccctcgatc
     1681 gtcccgctca tcggtggctc gtccggggat cagcatcccg aggacccggt tctccggatc
     1741 ggcccagtcc tcgtctgtca tctcctcccc gtcgagtcgc acccacatca cgtccttccg
     1801 gccgccgccg tcgaacgtcc cgctgaagaa cgaacggcgc cggagcaccg ggttcgatcg
     1861 gaagatctgc agggccgatc tcgtgaagtt cagcatcgcc ttgtcgcgat gctcccaatc
     1921 gaaccagctg acctcgttgt cctggccgta cgcgttgttg ttgccacgct gcgtccgggc
     1981 gatctcgtcg cctccgagga gcatccggac gccctgggag aagatcaacg tggcgatcag
     2041 gttccgcttc atacgctcgc gcagcttcac gacctccggc tgatccgacg ggccctccac
     2101 cccccagttg gcggagaagt tctcgtccga cccgtcccgg ttgccctcgc cgttcgcctc
     2161 gttgtgcttc gactcgaacg tgacgaggtc ctggagggtg aagccgtcgt gcgccgtgac
     2221 gaagttgacc gaggcgtacg tgttccggcc gctcgcctcg tagaggtcgc tcgatccgga
     2281 aagccggaac gcgagctccg gcagctgccc ggggtcgccg cgccagaatc gacgcacgca
     2341 gtctcggtac ttcccgttcc actccgccca tcccacgggg aagttgccca cctggtagcc
     2401 gccgggaccc acgtcccacg gctcggcgat cagcttcacc tgtgagagca ccgggtcctg
     2461 ctggatgatg tcgaagaacg tccccaggcg gttcacctcg tagagctcgc gagcgaggac
     2521 cggcgccaga tcgaaacgga acccgtccac gtgcatctcc agcacccagt ggcggaggct
     2581 gtccatgatc agctggatcg cgcgcggatg cagcatgttg aggctgttgc ccgtccccga
     2641 gaagtcgatg tagtagcgcg gatcgtcggg agagagccgg tagaaggcct tgttgtcgat
     2701 cccccggagg gagagcgtcg ggccgaggtg attcccttcg gccgtgtggt tgtagacgac
     2761 gtcgaggatc acctcgatgc cggcccggtg caacgtcttg accatcgact tgaattcata
     2821 gacctgctgc ccgacggagc gggtggcata ggcgtggtgc ggggcgaaga acccgatcga
     2881 gttgtagccc cagtagttcc cgaggccgcg ctcgacgagg tgccggtcgt cgacgaactg
     2941 atgcacgggg aggagct
//
LOCUS       DASUVE010000334         3406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_918234, whole genome shotgun sequence.
ACCESSION   DASUVE010000334 DASUVE010000000
VERSION     DASUVE010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918234"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="VF036_07225"
     CDS             <1..146
                     /locus_tag="VF036_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005484153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6265056.1"
                     /translation="VWVNCFYVRDLRVPFGGSRNSGIGREGGFWSFDFYADVKNVVTA
                     PWD"
     gene            172..1071
                     /locus_tag="VF036_07230"
     CDS             172..1071
                     /locus_tag="VF036_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 1,2-dioxygenase"
                     /protein_id="HEX6265057.1"
                     /translation="MGEIVGAGIVSHVPTIMLPEETRLEINDGKEITLVPGLRRLRSE
                     VLDRIGADTFVVMDTHWEVTFEHIITGHEKREGIFTSHELPRGMRQIPYDMPGDPELA
                     FGVSQQAEGRDDTWVLASDDPYLPIFYGTVNIWSYLNEGPENWVSVGINQCGTTEDFL
                     LLGELIGEAVVSLDRKVVLLASGGLSHRFLPFRELRGRESSDPMKNIITPEAREADLH
                     VLDLLQKGDHAAVIDWVPEYKKVGPEGKFGHYLIMAGALGGAACTAPGELFSEYEASV
                     GTGQVHMWFERPATGWTGSTEAA"
     gene            1111..2592
                     /locus_tag="VF036_07235"
     CDS             1111..2592
                     /locus_tag="VF036_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6265058.1"
                     /translation="MATEMDNMTDGSGYERLGGRRLKLVDVIAQSVGLIGPVFSAAFL
                     IPLIAGFGFSGEGAGVATPFAVVLAAIGVLALGWIVAQYAKRVHAAGSLYDYVTMGLG
                     SRVGGWAGWVYYGGTLLLSSAIAVLVGWFLRDNVLPAYGIDPIMPSWAWSMIFVALVF
                     LILAAGVQISTRLQFVLALVSAAVVLAFLVTVIIDAPENSLRAFNPAEAPSYTGILFG
                     VLYGVLIFVGFESAANLAEETERPKRAIPRAVTLSVLIVSVFYVIVAYAYVAGFGFDM
                     SVLLDPAIASGPLFALGSPDFYGSDFMLDLLQLVVLLDVMAVGLGAATASTRGLFALA
                     RDRKIPGGAAKTTSGGNPIGAALVVAVVSAAWVLATFGFEGLFANGVLVPVPHELGVF
                     QWISTLGAFLIMVVYGFLAIGAFAGLSDHPNRVGVVISGLVGLAVAVGAIFGAIYKVP
                     APFDVVWRWAAVWGVLGLLVTLAVKGREPARRALADLSTGEEG"
     gene            2612..>3406
                     /locus_tag="VF036_07240"
     CDS             2612..>3406
                     /locus_tag="VF036_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6265059.1"
                     /translation="MTDPDRPLRLDAPREEALEHVAKLVAEAWRSFDKPRPEEPPLDE
                     RLRAILRAGLPAGPVSVHEALDDAARAMDESIAQPRPRYFAFIGSSGLEIGALGDLLA
                     ATYDMNLAVDARAATVLEQQAVRWVGEFIGYPARNGAFTSGGTISNMTALAAARERAL
                     PGSRAAGLGGLRPAVYASAEVHYSITRAVELLGIGSDRLRDVPIDAEHRMRPELLAEA
                     IDRDVADGVTPVAVVATAGTTLTGAIDPLASIAEICETRGIWFHVDG"
BASE COUNT          487 a         1098 c         1231 g          590 t
ORIGIN      
        1 ccgtgtgggt caactgcttc tacgtgcgcg acctgcgtgt gccgttcggc gggtcgcgga
       61 acagcgggat cggtcgcgag ggcgggttct ggagcttcga cttctacgcg gacgtgaaga
      121 acgtcgtcac ggcaccctgg gactgaccaa gacctgagag gagcaccgac catgggagag
      181 atcgtcggcg cggggatcgt ctcgcacgtc cccacgatca tgctgccgga ggagacccgg
      241 ctcgagatca acgacgggaa ggagatcacg ctcgtaccag ggctgcggcg gctccggtcc
      301 gaggtcctcg atcgcatcgg ggccgacacg ttcgtcgtga tggacacgca ctgggaggtg
      361 acgttcgagc acatcatcac ggggcacgag aaacgcgagg gcatcttcac atcgcacgag
      421 ctgccacgcg ggatgcggca gatcccctac gacatgccgg gcgatcccga gctcgcgttc
      481 ggcgtctccc agcaggccga agggcgcgac gacacctggg tgctcgcctc cgacgatccg
      541 tacctcccga tcttctacgg cacggtgaac atctggtcct acctgaacga gggaccggag
      601 aactgggtga gcgtcggcat caaccagtgc gggaccaccg aagacttcct gctcctcggg
      661 gagctcatcg gtgaggcggt cgtctcgctc gatcgcaagg tggtgctgct ggcgagcggc
      721 gggctgtccc accgcttcct gccgttccgc gagctccggg gccgagagag ctccgatccc
      781 atgaagaaca tcatcacgcc cgaggcgcgc gaggcggacc tgcacgtcct cgacctcttg
      841 cagaaggggg accacgccgc ggtgatcgat tgggtgcccg aatacaagaa ggtcggcccg
      901 gaggggaagt tcgggcacta cctgatcatg gccggcgcgc tcgggggagc ggcgtgcacc
      961 gcgccgggcg agctgttcag tgagtacgag gcttcggtcg ggaccgggca ggtgcacatg
     1021 tggttcgaac gcccggcgac cggttggacc ggctcgaccg aagccgcata gggttcgcgc
     1081 cagggactgg cggacgagag ggggagttgc gtggcaacgg agatggacaa catgacggac
     1141 gggtccgggt acgaacgcct tggggggcgt cgcctcaagc tcgtcgacgt catcgcccaa
     1201 tcggtcggtc tgatcggccc ggtgttctcg gcggcgttcc tcatcccgtt gatcgccggg
     1261 ttcggattct ccggtgaagg ggccggcgtc gcgacgccgt tcgcggtggt cctggcggcg
     1321 atcggcgtcc tcgcgctggg gtggatcgtg gcccagtacg cgaaacgcgt gcacgccgcg
     1381 gggtcgctct acgactacgt gacgatgggg ctcggctcac gggtaggtgg gtgggccggg
     1441 tgggtctact acggcggcac gctgctactc tcctccgcga tcgccgtcct ggtcgggtgg
     1501 ttcctccgcg acaacgtgct tcccgcgtac gggatcgacc cgatcatgcc ctcgtgggcg
     1561 tggtcgatga tcttcgtcgc actcgtgttc ctgatcctag cggctggcgt gcagatctcg
     1621 acccgtctcc agttcgtgct ggcgctcgtg tcggccgccg tcgtgctggc gttcctcgtg
     1681 accgtgatca tcgatgcgcc ggagaacagc ctcagggcgt tcaaccccgc cgaggcgccc
     1741 agctacacgg gcatcctgtt cggcgtgctg tatggcgtgc tgatcttcgt cggcttcgaa
     1801 tcggccgcga acctcgcaga agaaaccgag cggccgaagc gggcgatccc gagagcggtg
     1861 accctttcgg tcctgatcgt ctcggtcttc tatgtgatcg tggcgtacgc ctacgttgcg
     1921 gggttcggct tcgatatgtc ggtcctgctc gacccggcga tcgcctctgg cccgctattc
     1981 gcgctgggtt ccccggactt ctatggctca gacttcatgc tcgacctgct ccagctggtc
     2041 gtcctgctgg acgtgatggc ggtcggcctt ggcgccgcca ctgcctccac gcgaggactc
     2101 ttcgcgctcg cgcgcgaccg caagatcccc gggggcgcgg cgaagacgac gagcggcggc
     2161 aacccgatcg gtgcggcgct cgtcgtcgcg gtcgtttccg ctgcctgggt actcgccacc
     2221 ttcgggttcg aggggttgtt cgcgaacggt gtgttggtgc cggtcccgca cgagcttggg
     2281 gtgttccaat ggatctcgac tctcggcgcg ttcctgatca tggtcgtgta cggcttcctc
     2341 gccatcggcg cgttcgcggg cctgagcgac cacccgaacc gtgtgggcgt ggtgatctcg
     2401 ggtctcgtcg ggctcgcggt cgcggtagga gccatcttcg gggcgatcta caaggtgcct
     2461 gcgccgttcg acgtcgtctg gcgctgggcg gcggtgtggg gcgtgctcgg tctgctcgtc
     2521 acgctcgccg tgaagggccg cgagccggct cgcagagcgc tcgcggacct ctcgacaggc
     2581 gaggaaggct agcgggcttc gacacaggaa gatgaccgac ccggaccggc cgctgcggct
     2641 cgacgcgcct cgcgaggagg cgctcgagca cgtggcgaag ctggtggccg aggcctggcg
     2701 gagcttcgac aagcctcggc cggaggagcc gccgctggac gagcggctcc gagcgatcct
     2761 gcgggccggg ctccccgcgg gaccggtgag cgtccacgaa gcgctggatg acgcggcgcg
     2821 agcgatggac gagtcgatcg cgcagccgcg tccgaggtac ttcgcgttca tcggttcctc
     2881 cggcctcgag atcggagcgc tcggcgacct gctcgctgcg acgtacgaca tgaacctcgc
     2941 cgtcgatgcg cgggcggcca cggtcctcga gcagcaggcc gtccgatggg tcggcgagtt
     3001 catcgggtac ccggcgagga acggcgcgtt cacgagcggc ggcacgatct cgaacatgac
     3061 ggcgctcgcg gcggcacgcg agcgggcgtt gcccggctcg cgcgcggccg gactcggcgg
     3121 tctccgcccc gccgtctacg catcggccga ggtgcactac tcgatcaccc gcgcggtcga
     3181 gctgctcggg atcgggagcg atcggctccg cgacgtcccg atcgacgccg agcatcggat
     3241 gcggcccgaa ctgctggcgg aggcgatcga tcgggacgtt gccgacggtg tgacgccggt
     3301 cgccgtggtc gcgaccgccg ggacgacgct gaccggcgcg atcgacccgc tcgcgtcgat
     3361 cgcagagatc tgtgagacgc gcgggatctg gttccacgtc gacggg
//
LOCUS       DASUVE010000335         5310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_354453, whole genome shotgun sequence.
ACCESSION   DASUVE010000335 DASUVE010000000
VERSION     DASUVE010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354453"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VF036_07245"
     CDS             complement(<1..287)
                     /locus_tag="VF036_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265060.1"
                     /translation="MADDFQFGERAKHPARLGGPRPTRPKSSIPFKLAGVVVVLVVTA
                     AAAFVFLRGADEAGTETADARSETLAQIDRAYDAAAQGTMGRAVVVAQTLYA"
     gene            405..1544
                     /locus_tag="VF036_07250"
     CDS             405..1544
                     /locus_tag="VF036_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX6265061.1"
                     /translation="MSEIGDVLAAIEALSAKGERMALATIVAVRGSTYRRPGARLLVP
                     EEGAPVGNLSGGCLEGDVADMARVVMEEGRARLAGWDLTADDDAVWGLGLGCNGAIEV
                     FIEPAEKAAEVAGALRAALDAERPISVVTVVESSSPDVEPGSRLVVRPDGTTDGSLGT
                     AAVDVEAVRAAEALLREERSEVRILADGVRAFVEVLDPPLRLLVCGAGHDAIPLVRAA
                     SVLGWNVMVADDRPAFLTRERFPEASGFVAVDDPADAAKEAGVDDRTFVVVMSHHFLR
                     DKEYVRSMLGSPAAYVGTLGPGARTERLLMELRDEGVEVGDAGRQRIHGPAGLDLGGE
                     GPEEIAQAIVAEIVAVKRHRSGGFLRDRPGPIHDRPRPGTEARPG"
     gene            complement(1557..2435)
                     /locus_tag="VF036_07255"
     CDS             complement(1557..2435)
                     /locus_tag="VF036_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX6265062.1"
                     /translation="MDFRKGAKLDPTQVEDRRGMRGVRGGRGMAVGGGAGVVGVIVVL
                     LITLLGGDIDLSALQSLDGVIAGGQQQQGEVIEECKTGEDAERSRDCRLIGTVNSIQA
                     YWAGAVEGYTPSVTVFFTDSTGTGCGAATADVGPFYCPADKKVYVDLGFFEDLQTKFG
                     AEGGPFAEAYVLAHEYGHHVQDLLGVLDQIGGDREGPQSAAVRTELQADCYAGVWAAN
                     AVDTRFLASLGEDEITQALDAAASVGDDRIQRETQGQVNREAWTHGSSLQRQRWFMTG
                     YRGGDPNDCDTFSGTV"
     gene            complement(2479..2691)
                     /locus_tag="VF036_07260"
     CDS             complement(2479..2691)
                     /locus_tag="VF036_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265063.1"
                     /translation="MWVVVAAAVTWVVSSAVSSLLYLQVGATLFEGTGIFDTLVRWSQ
                     LISLVAQSLTLGGSAVYVVFWLQNRR"
     gene            3103..3972
                     /locus_tag="VF036_07265"
     CDS             3103..3972
                     /locus_tag="VF036_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6265064.1"
                     /translation="MLPAPFEYHRPETLEEALQLLAEHGEDAKVLAGGQSLIPLMKLR
                     FAAPGRLIDVNRIAGLDGIEETDGFLRIGALVRHNALAASEVIKERYPTIAVAAPMIS
                     DPIVRNLGTIGGSLAHADPSGDLGSVMLALGATVVVRSGDGEREIAAVDLADGPFQSS
                     IAPNELLTEIRVPSAPRRSGGTYLKLERRVGDFATVAVAICLTLENGSIGRAGIGLTS
                     VGLTNLKANAAEASLVGASPGESAFAEAGRLAAAAAEPATDVRGSADYKRHVVDVYVR
                     RGLAAATEMAGAA"
     gene            3992..4477
                     /locus_tag="VF036_07270"
     CDS             3992..4477
                     /locus_tag="VF036_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6265065.1"
                     /translation="MEVTVNVNGVERSADVEPRQLLVHLIREEFELTGTHIGCDTTSC
                     GACTVLLDGVPVKSCTMFGVQADGRSVTTVEGLAGAAGLHPIQAAFKEEHGLQCGFCT
                     PGMMLTGAALIEQNPTPSEQDIRWAISGQMCRCTGYTNIVKAIQAAAAATSGMSTPAA
                     V"
     gene            4506..>5310
                     /locus_tag="VF036_07275"
     CDS             4506..>5310
                     /locus_tag="VF036_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6265066.1"
                     /translation="MATETEEIRGIGHRVRRVEDDRFIRGAGNYIDDIHLPGMLHMAI
                     LRSPYAHANLKGIDASRAQALPGVIAVVTGELMAQHNLAWMPTLSGDTQAVLVTDRVR
                     FQGQEVAAVIADDPYVAHDALELIDVDYDPLDAVTTPQQALEDGAPLIRTDKEGQENN
                     HVYSWETGNKEATDIAFSQADVVVSLDTFYPRSHPAPLETCGCVADISPATGQATIYM
                     TSQAPHLIRTVFALVAGLPEDKIRIVSPDSVGIQARLCCAISSPVTTAMT"
BASE COUNT          844 a         1847 c         1844 g          775 t
ORIGIN      
        1 gcgtacaggg tctgtgccac caccaccgcc cgtcccatgg tcccctgcgc cgccgcgtcg
       61 tacgcccgat cgatctgcgc gagggtttcc gaccgtgcgt cggcggtctc cgttccggcc
      121 tcgtcggcac cccggaggaa cacgaacgca gctgctgcgg tcacgacgag gaccacgacg
      181 acccccgcca gcttgaacgg gatcgagctc ttcggccggg tcggccgagg ccctcccagg
      241 cgcgcgggat gcttcgcacg ctcgccgaac tggaagtcgt ccgccatgtc tcccagtatc
      301 ggtcgagccc ggcgccgcca tgagcgggac cgatctcgtc cacctgtctc tgcgagcagg
      361 ggctcggaag atcgaaggtc cgtcgccggg ctaccatcgg ctccatgagc gagatcggcg
      421 acgtgctggc ggcgatcgag gcgctctccg ccaagggcga gcggatggcg ctcgcgacga
      481 tcgtggccgt ccggggatcg acctaccgca gaccgggcgc ccggttgctc gtccccgagg
      541 agggcgcccc cgtcgggaac ctgtccgggg gctgcctcga gggcgatgtc gccgacatgg
      601 cccgggtggt catggaggag ggacgcgccc gcctggcggg gtgggatctg accgccgacg
      661 acgacgcggt ctgggggctc ggtctcggat gcaacggcgc catcgaggtc ttcatcgaac
      721 ccgcggagaa agctgccgag gtggccggcg cgctgcgcgc ggcgctcgac gcggagcggc
      781 cgatcagtgt cgtgacggtg gtcgagtcgt cctccccgga cgtcgaaccg gggtcgcggc
      841 tcgtcgttcg ccccgatgga acgaccgacg gctccctcgg aaccgccgct gtggatgtcg
      901 aggccgttcg cgccgcggag gcgctgctcc gggaggaacg ctcggaggtg cggatcctcg
      961 ccgacggcgt ccgcgcgttc gtcgaggtcc tcgacccgcc gcttcggctc ctggtctgcg
     1021 gcgcgggcca cgacgcgatc ccactcgtcc gtgcggcctc ggtcctgggg tggaacgtca
     1081 tggtggccga cgatcgcccg gcgttcctga cgcgcgagcg gttccccgag gcgagcggct
     1141 tcgtcgcggt cgacgacccc gcggacgctg ccaaggaggc aggggtcgac gaccgcacgt
     1201 tcgtggtcgt gatgtcgcat cacttcctcc gcgacaagga gtacgtccgg tcgatgctcg
     1261 gttcgcctgc ggcgtacgtc ggcacgctcg gtccgggggc gaggacggaa cgcctgttga
     1321 tggagctccg cgatgaaggt gtcgaggtcg gggacgccgg ccgacaacgg atccacgggc
     1381 ccgccggcct cgacctcggc ggcgaaggcc cggaggagat cgcgcaggcc atcgtcgcgg
     1441 agatcgtcgc ggtcaagcgg caccgctcgg gcggctttct ccgtgaccgc cccggaccga
     1501 tccacgaccg gccccggccg ggaacggaag ctcgccccgg ttgatgcgtc gtcgggtcag
     1561 acggtgccgc tgaacgtgtc gcagtcgttc gggtcgccgc cccggtagcc ggtcatgaac
     1621 caccgctggc gttgcagaga cgagccgtgc gtccacgctt cgcggttgac ctgcccctgg
     1681 gtctcccgct ggatgcgatc atcgccgacc gacgccgcgg catccagcgc ctgcgtgatc
     1741 tcgtcctcac cgagcgatgc gaggaagcgg gtatccaccg cgttcgcggc ccagacgccg
     1801 gcgtaacagt cggcctggag ctccgtccgc acggcggcgc tctgtggacc ctcgcggtcc
     1861 ccgccgatct ggtcgagcac accgagcagg tcctgcacgt ggtgcccgta ttcgtgcgcc
     1921 agcacgtagg cctctgcgaa cggtccgcct tccgccccga acttcgtctg cagatcctcg
     1981 aagaacccga ggtcgacgta gaccttcttg tcggcggggc agtagaacgg gccgacgtca
     2041 gcggtcgccg ccccgcaccc ggtccccgtg gagtcggtga agaacaccgt cacggagggc
     2101 gtgtatccct cgacggcccc ggcccagtag gcctggatac tgttgacggt gccgatcagg
     2161 cggcagtcgc ggctccgctc ggcgtcctcg ccggtcttgc actcctcgat gacctcgccc
     2221 tgctgctgct ggcctccggc gatcacgcca tcgagggact gcagcgcgct caggtcgatg
     2281 tcgcccccga gcagggtgat caggaggacg acgatgacac ccacgacacc cgcgccaccg
     2341 ccgacggcca tgccgcggcc accgcgcacc ccacgcatgc cacgacggtc ctcgacctgc
     2401 gtgggatcga gcttcgcgcc cttccggaag tccacggggg ctcgataccc gaacgcgttg
     2461 cgcgggaaac gagaaggtct accggcggtt ctggagccag aacacgacgt agacggcgga
     2521 tccgccgagc gtcaaagact gggcgacgag gctgatgagc tgcgaccacc ggacgagggt
     2581 gtcgaagatc cccgttccct cgaacagggt cgcgcccacc tgcaggtaca ggagcgacga
     2641 gacggcggag gagacgaccc aagtgacagc ggctgcgacg acgacccaca cagcatccgg
     2701 agccagcgat cacggcgagc gctcgccgtg agctctttcc accgagcggc cgctgcccgg
     2761 aagcctcgat cgtcgtcgga gaaccgctcg atgggatcgc cgtcatccag atcgtcgagc
     2821 ctccagacgc cgtatccctg gtcgtcgcgc accacgacga accgcgcgtt ggcgtcgaac
     2881 gtctcgaacc ggccgccgcc ggtcgctcgc tcatcgggct cctctcgaag gtggctgcac
     2941 gctaccccgg cagctcgccg gcagcgagga tcgtcgattt gccggtcgcg cccggcgggg
     3001 ggaggattgg cgctctccgg aaagctgcac gcctcccgga gccgtatcac catggtcggg
     3061 tcgccgtccc agcggcccct gcggagatcg ggggttcagg agatgctccc agcaccgttc
     3121 gagtaccacc ggcccgagac cctcgaggag gcgctccagc tcctcgcaga gcacggagag
     3181 gatgcgaagg tcctcgccgg aggtcaaagc ctgatcccgc tgatgaagct ccgcttcgcg
     3241 gcaccgggtc ggctgatcga cgtcaatagg atcgcgggac tggacgggat cgaggaaacc
     3301 gatgggttcc tgaggatcgg ggcgctggtc cgccacaatg cgctcgccgc gtccgaggtg
     3361 atcaaggaac gctatcccac gatcgccgtg gcggctccga tgatctccga cccgatcgtg
     3421 cgaaacctcg gcacgatcgg cggatcgctc gcacacgcgg atccctccgg cgacctgggg
     3481 tcggtgatgc tcgctctcgg agccacggtc gtcgtccgca gcggcgacgg cgagcgcgag
     3541 atcgccgccg tcgacctggc ggatggacca ttccagagct cgatcgcccc gaacgagcta
     3601 ctcacggaga tccgcgtccc ctcggcgccg cgaaggtccg gcggcaccta cctgaaactc
     3661 gaacggaggg tcggggactt cgcaaccgtg gccgtcgcga tctgcttgac gctcgagaac
     3721 ggatcgatcg gccgagccgg tatcggtctt acctcggtgg gcctcacgaa cctgaaggcg
     3781 aacgccgccg aggcctctct cgtgggtgct tctcccggcg agtccgcctt cgccgaggcc
     3841 ggacgcctcg cggcagcggc ggcggaaccg gcgaccgatg tccgcggctc ggccgactac
     3901 aagaggcacg tcgtggacgt gtacgtgcgc cgaggtctcg cggccgcgac cgagatggcc
     3961 ggcgcggcct gagcccgaga ggagacgcga catggaggtc acggtcaacg tcaacggcgt
     4021 ggagcgatcg gcagacgtag agcctcgcca gttactggtc cacctgatcc gcgaggagtt
     4081 cgagctcacc ggaacgcaca tcggctgcga cacgaccagc tgcggagcgt gcacggtgct
     4141 cctcgacgga gtgccggtca aatcgtgcac gatgttcggt gtccaggccg acggtcgctc
     4201 ggtcacgacc gtcgagggtc tggccggggc ggcggggctc cacccgatcc aggcggcgtt
     4261 caaggaggag catggtctgc agtgcgggtt ctgcaccccc ggcatgatgc tcaccggggc
     4321 cgcgctcatc gaacagaatc cgacgccgag cgaacaggac atccgctggg cgatctcggg
     4381 gcagatgtgc cgttgcaccg gttacacgaa catcgtgaag gcgatccagg ctgcggccgc
     4441 ggcgacgtcg ggtatgagca cgcctgccgc cgtctagcca ccctgaccag aaggagccga
     4501 gggagatggc gacagagacc gaagagatcc gcgggatcgg acaccgcgtc cgccgcgtgg
     4561 aagacgaccg gttcatccgt ggcgccggca actacatcga cgacatccac ctccccggga
     4621 tgctgcacat ggcgatcctg cggagcccgt acgcccacgc gaacctcaag ggcatcgatg
     4681 cgtcgagggc gcaggcgctc cccggggtca tcgccgtcgt caccggcgag ctgatggcac
     4741 agcacaacct cgcctggatg ccgacgctgt cgggcgacac gcaggcggtc ctggtgaccg
     4801 accgggtgcg gttccaggga caagaggtcg cggcggtcat cgccgacgac ccctacgtcg
     4861 cgcacgacgc gctcgagctg atcgacgtcg actacgatcc gctcgacgcg gtgacgaccc
     4921 cgcaacaggc gctggaggac ggcgccccgc tgatccggac cgacaaggag ggccaggaga
     4981 acaaccacgt ctactcgtgg gagaccggca acaaggaggc gacggacatc gcgttctcgc
     5041 aggccgacgt cgtcgtctcg ctcgacacgt tctacccgcg gagccatccg gcgccgctcg
     5101 agacgtgcgg atgcgtcgcg gacatcagcc ccgcgaccgg ccaggcgacg atctacatga
     5161 cctcgcaggc gccgcacctc atccggaccg tgttcgcgct ggtggccggc ttgcccgaag
     5221 acaagattcg gatcgtgtcg cccgacagcg tcggcatcca ggcgaggttg tgctgtgcca
     5281 tcagctcgcc ggtgacgacg gcgatgaccc
//
LOCUS       DASUVE010000336         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_918841, whole genome shotgun sequence.
ACCESSION   DASUVE010000336 DASUVE010000000
VERSION     DASUVE010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918841"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..987
                     /gene="atpA"
                     /locus_tag="VF036_07280"
                     /pseudo
     CDS             1..987
                     /gene="atpA"
                     /locus_tag="VF036_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007147732.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
     gene            1230..2123
                     /locus_tag="VF036_07285"
     CDS             1230..2123
                     /locus_tag="VF036_07285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX6265067.1"
                     /translation="MGAQLRIVRRRIRSVQSTKKITRAMELIASSRIVKAQQRVESSR
                     PYAIQLTKAMEDLARQTGSVQHPLLDDRENPSKAGILVVTSDRGLAGAYNANVLKVAE
                     GVFRDVRNRGLEPVLYVIGKKGVGYFRFRGVPMRKSWQGFSEVPPYEEAERVGRVLIK
                     DFADEEIDELHAAYTDFRSAFTLRATTKRFLPIAPEEVTGKGLGVVHPEYLFEPEPEE
                     ILDHLLPQYVVTKVYAGLLDSAASENAARRRAMKAATDNAEDLVKILTRQANRARQDE
                     ITTEIMEIVGGAESMAAGSEG"
     gene            2126..>2664
                     /locus_tag="VF036_07290"
     CDS             2126..>2664
                     /locus_tag="VF036_07290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016667716.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX6265068.1"
                     /translation="MPGTNEKAHPEGRVVRVIGPVIDVEFPPSELPEVHTALTVDLTL
                     EGESTTITCEVAQHIGDSIVRTIALKPTDGLVRGTPVKNTGSPITVPVGEGVLGHVYN
                     VIGEPLDADPSEIHASDRWPIHREPPEFAELEPKKQMLETGIKVIDLLEPYVQGGKIG
                     MFGGAGVGKTVIIQEMIRR"
BASE COUNT          487 a          863 c          937 g          377 t
ORIGIN      
        1 gccgtcgacg cgatcatcgc gcagaaggag ctctgggcga ccggggatcc caagaaccgg
       61 gtgaagtgca tctacgttgc ggtcggccag aagtcctcga cggtcgccga ggtcgtggcg
      121 accctggagg agaacggcgc gctggagtac acggtcgtcg tgaacgctcc ggcatcggac
      181 ccggccccgt tccagtacat cgccccgtac tccggtgctg cgttcggtgc gcactggatg
      241 tacaacggcg agcacgcgct gatcgtctac gacgacctgt cgaagcaggc caccgcgtat
      301 cgaacgttgt cgctcctgct gcgccgcccg ccggggcggg aggcgttccc cggggacgtc
      361 ttctacctgc actcgaggct gctcgagcgt gccgcgaagc tggcggacga cctcggcggc
      421 ggctcgctca ccgcgctccc gatcatcgag acgaaaggca acgacatctc ggcctatatc
      481 ccgacgaacg tcatctcgat cacggacggg cagatcttcc tcgagccgga cctattcttc
      541 gcgggcgtcc ggccggcgat caacatcggc atctcggtct cgcgcgtcgg cggagccgcg
      601 cagatcaagg cgatgaagaa ggtcgccggg cgactccgac tcgacctcgc gcagttccgg
      661 gcgctggagg cgttcgcgca gttcggctcc gagctggaca aggcctcgca gcaacagctc
      721 gcccgcggcg cgcgggtcgt cgaggtcctg aagcagcctc agtacgaacc gctgcccgtc
      781 gagcgcgagg tcgtgatgat ctggaccgcc accggcggct atctcgatcc gttccccgtc
      841 gaggacgcga ggcgcttcgt cgaggagttc acgacgttcc tcgactcccg gaccgacgtc
      901 ctcggcgaga tcaaagagac cggcgacatg tccgacgaga cggagaagac gctgaaggag
      961 tcggtcgagg cgttcgcgca gtcgttccag accagcgaag cggccgcagg cagcgaggcc
     1021 ggccgcggtg ccgggacacc gcccgacgag gtcaagccgg acatcggatg ggaccggatg
     1081 tcttcggtcg acgacgagga agaggaagag gacggcggtc cttcattggc agaagaggac
     1141 gacgtcgagg gccaggtgcc cctgccagat gatgaagaag ccgacgaggg cactgatgac
     1201 gcgaaggcag cgggagaccc ggaggagtaa tgggagccca gcttcggatc gtccgccggc
     1261 ggatccgctc ggtccagtcg acgaagaaga tcacgcgtgc gatggagctc atcgcgtcct
     1321 cgcgcatcgt gaaggcgcag cagcgcgtgg agtcgtcccg cccgtacgcg atccagctga
     1381 ccaaggcgat ggaggacctc gcgcggcaga cgggctcggt gcagcatccg ctgctcgatg
     1441 accgcgagaa cccctcgaag gcgggcatcc tggtcgtgac gtcggaccgg gggctcgcgg
     1501 gcgcgtacaa cgccaacgtg ctgaaggtcg cggagggcgt gttccgcgac gttcgcaacc
     1561 gaggactgga gcccgtcctg tacgtgatcg gcaagaaggg cgtcggctat ttccggttcc
     1621 gaggcgtgcc gatgcgcaag agctggcagg ggttcagcga ggtccccccg tacgaggagg
     1681 cggaacgcgt gggccgcgtc ctgatcaagg acttcgcgga cgaggagatc gatgagctcc
     1741 acgctgccta cacggacttc cgctccgcgt tcacgctccg cgccacgacg aagcggttcc
     1801 tgccgatcgc tcccgaagag gtcaccggga agggcctcgg cgtcgtccac cccgagtacc
     1861 tgttcgagcc ggagccggag gagatcctgg atcacctcct cccgcagtac gtggtgacga
     1921 aggtctacgc ggggctgctc gactcggcag cgtcagagaa cgctgcccgg cgccgggcga
     1981 tgaaggccgc gaccgacaac gcggaggacc tggtgaagat cctgacgcgg caggcgaacc
     2041 gcgcgcgcca ggacgagatc acgaccgaga tcatggagat cgtgggcgga gccgaatcga
     2101 tggctgccgg ctcggaggga tgacgatgcc aggaacgaac gagaaggcgc acccggaggg
     2161 gcgcgtggtc cgggtgatcg gcccggtgat cgacgtggag ttcccaccct ccgagctccc
     2221 cgaggtccac acggcgctca ccgtcgacct gaccctggag ggtgagtcga cgacgatcac
     2281 ctgcgaggtc gcgcagcaca tcggcgactc gatcgtccgc acgatcgccc tgaagccgac
     2341 cgacggcctg gtgcgcggca cccccgtcaa gaacacgggg tccccgatca ccgtccccgt
     2401 gggggagggc gtgctcggcc acgtctacaa cgtgatcggc gagcccctcg acgcggaccc
     2461 gtccgagatc cacgcgagcg accgctggcc gatccaccgg gagccgccgg agttcgcgga
     2521 gctcgagccc aagaaacaga tgctcgagac cgggatcaag gtgatcgacc tcttggagcc
     2581 gtacgtgcag ggtggcaaga tcggcatgtt cggcggcgcc ggcgtcggca agaccgtgat
     2641 catccaggag atgatccgcc ggca
//
LOCUS       DASUVE010000337         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_544537, whole genome shotgun sequence.
ACCESSION   DASUVE010000337 DASUVE010000000
VERSION     DASUVE010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544537"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VF036_07295"
     CDS             complement(<1..303)
                     /locus_tag="VF036_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX6265069.1"
                     /translation="MESLGKAAVAEFVATFALIFVGAGAVIVASSGQLDLVGVALAHG
                     FVLAVMVSVTGHISGGLVNPAVTIALWTAGRIGTQRGAVLIVAQMVGAVLGALLLRY"
     gene            456..896
                     /locus_tag="VF036_07300"
     CDS             456..896
                     /locus_tag="VF036_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265070.1"
                     /translation="MEASSIARVDVLLVSPDVRSRELMALAVRSIERRLGEEIRFRAA
                     RNGELGTWAALRERPDMIVADEIASREGAFGLARTLRDHPEPYTGVIVILLDRKHDVW
                     LAKWSGADAWFVKPVDPFELADRLLDLVTESETKTEPRTEKESA"
     gene            893..1180
                     /locus_tag="VF036_07305"
     CDS             893..1180
                     /locus_tag="VF036_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265071.1"
                     /translation="MKIKGTCKRDGRDFFAEQVVASGGECPWDGQPFNADYAAVLVES
                     LRAAERLGTELERALERVASLHPAFTLDEHSVLGDLTDQLGRFHRNLVRQA"
     gene            1192..1413
                     /locus_tag="VF036_07310"
     CDS             1192..1413
                     /locus_tag="VF036_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265072.1"
                     /translation="MTADGNGFAVEVRGGEATWTVAIVSPEGEVVSERACRDGAEART
                     YASTVRQHIFWLSPEKFREYYRIEGQVEG"
     gene            1521..1874
                     /locus_tag="VF036_07315"
     CDS             1521..1874
                     /locus_tag="VF036_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6265073.1"
                     /translation="MTDGRSLADLDYCYLTTTGRRTGGPHRIEIWFALGDGVVYLLSG
                     GGDRSDWVRNLEVSPDVVLEIGGETRTTQARVVTDPQEDALARRLVADKYRPRSRGDL
                     DDWERTSVPVAIEWR"
     gene            complement(1871..2158)
                     /locus_tag="VF036_07320"
     CDS             complement(1871..2158)
                     /locus_tag="VF036_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018837650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX6265074.1"
                     /translation="MTVHAVVLIQCEIDEIPEAAEAIAEIGGVSEVYSVAGEFDLVAV
                     VRVAGHDELETVIPGGISKVGGVERTETLIAFQVYSKHDLERMFSIGFEER"
BASE COUNT          358 a          758 c          808 g          328 t
ORIGIN      
        1 gtaccggagc aggagagcgc ccagcaccgc accgaccatc tgcgccacga tcaggaccgc
       61 gccccgctgg gtgccgatcc tgccggccgt ccacagggcg atcgtcaccg ccgggttgac
      121 gaggccgccg gagatgtggc ccgtgacgga gaccatgacg gcgaggacga agccgtgggc
      181 gagcgccacg cccaccaggt cgagctgacc cgagctcgcg acgatcaccg cgccggcgcc
      241 cacgaagatc agcgcgaacg tggccacgaa ctccgcgacc gccgccttcc cgagactctc
      301 catcacgtcc tccttggtgg tcgctccgac ggttgctcgg cgctgctcga agcacgagag
      361 cgcgtggcac ggtgcctgcc gcgtcccgac gcgcccgagc ctagcccgcc gttcggggcc
      421 gttcaaagac cgcgagatcc gcctccgagg gccgcatgga agcgagtagc atcgcccgcg
      481 tggatgtcct gctggtgtcc ccggacgtgc gctcccggga gctgatggcc ctcgcggtca
      541 ggagcatcga gcgccggctg ggcgaggaga tccgcttccg tgcggctcgc aacggcgagc
      601 tcggcacctg ggcggccctc cgggagcggc cggacatgat cgtcgcggac gagatcgcct
      661 ctcgcgaggg agcgttcggg ctcgcgagaa ccctccggga tcacccggag ccttacacgg
      721 gggtcatcgt gatcctgctg gatcgcaagc acgacgtctg gctcgcgaag tggtcgggcg
      781 ccgacgcgtg gttcgtgaag cccgtggatc cgttcgagct ggccgaccgg ctgctcgacc
      841 tcgtgaccga gtcggagacg aagaccgagc cgagaaccga aaaggagagc gcgtgaagat
      901 caaggggacc tgcaagcggg acggccggga tttcttcgcg gaacaggtcg tggcatcggg
      961 cggcgagtgt ccctgggacg ggcaaccctt caacgcggat tacgccgccg tgttggtgga
     1021 atcgctgcgc gccgccgaac ggctgggaac cgagctcgaa cgggcgctcg agcgggtggc
     1081 ctcgctccat ccggcgttca ccctggacga acacagcgtc ctgggggacc tgacggacca
     1141 gctcgggcgg ttccaccgga acctcgtccg acaggcctga cggtggtcgc cgtgacggcg
     1201 gacgggaacg gcttcgcggt ggaggtccgg ggcggcgagg cgacatggac cgtcgcgatc
     1261 gtgagccccg agggcgaggt cgtgtcggag cgggcgtgcc gtgacggcgc ggaggccagg
     1321 acgtacgcgt ccaccgtccg gcagcacatc ttctggctct caccggagaa gttccgcgag
     1381 tactaccgga tcgagggtca ggtcgagggg tagccgatgg ggttcaacgc gatgcagccg
     1441 ggcaaggtca agaaggcgga tctgatcatc gccggtgtgg cgctcgtcgt gatcgtcggg
     1501 ctcgtcctgt gggcggtgct gtgaccgatg gtcggtccct cgccgacctc gactactgct
     1561 acctgaccac caccggccga cgcacgggcg ggccccatcg gatcgagatc tggttcgcgc
     1621 tcggcgacgg cgtggtctac ctcctatccg gcggcggtga ccgatcggat tgggtgcgga
     1681 acctcgaggt ctcgcccgac gtcgtcctcg agatcggcgg cgagacgcgg acgacgcagg
     1741 cgcgagtggt gacggatccc caggaggacg cgctcgcccg acggctcgtc gccgacaagt
     1801 atcggcctcg ctcccgaggg gatctggacg actgggagcg aacgtcggtc cccgtcgcga
     1861 tcgagtggcg ctagcgctcc tcgaagccga tcgagaacat gcgctccagg tcgtgcttcg
     1921 aatacacctg gaacgcgatc agcgtctcgg tgcgctccac ccctccgacc ttggagatcc
     1981 ccccggggat cacggtctcg agctcgtcgt gcccggcgac ccggacgacc gcgaccaggt
     2041 cgaactcgcc cgccaccgag tagacctcgg agacgccgcc gatctccgcg atcgcttccg
     2101 cggcctcggg gatctcgtcg atctcgcact ggatcaggac gacggcgtgg acggtcatcg
     2161 gctcaggtcc tttcgaggac gacggcgagc ccttgcccgc cggagatgca gagggtggcc
     2221 aggccgagtg agaccccgcg cgcgcgcatc tg
//
LOCUS       DASUVE010000338         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_919356, whole genome shotgun sequence.
ACCESSION   DASUVE010000338 DASUVE010000000
VERSION     DASUVE010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919356"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VF036_07325"
     CDS             <1..184
                     /locus_tag="VF036_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265075.1"
                     /translation="GSFRWRWERSEDAGATWSALWVIEPAGVKSPLGMRERQGTSRNG
                     KETAPLGKNPLSAVRA"
     gene            201..1169
                     /locus_tag="VF036_07330"
     CDS             201..1169
                     /locus_tag="VF036_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6265076.1"
                     /translation="MLRFIARRLLYSVLVLVIISILTFLIFVKLPAGDPARRAVGRRT
                     DPQQIANAREAFGLDRPLIVQYGRFAKGLIPLPNTFLSEDVYYSFSNYLPVREEIWRR
                     LPVSAILATGAAVLWLLIGIPLGIASGVRPRSLSARWTMVLAIVGVSLPVFWLGQLLL
                     YVVWYKLHLAPSAGLEINASVWGSALKGKFLLPWITVAVGYAAIYARMLRSNMIETTQ
                     EDYMRTARAKGLSERRVIYKHGLRGALTPIVTMLGVDFATILAGLVITETLFGLPGLG
                     QLAVRSIYSNDFPMVMGVTVVGSMFILAANIVVDVAYAFLDPRVRY"
     gene            1316..>2534
                     /locus_tag="VF036_07335"
     CDS             1316..>2534
                     /locus_tag="VF036_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6265077.1"
                     /translation="MADAYQMFIDGEWVNSSDGQTRDVINPATGEVIASVQEGTKEDA
                     NRAVAAARKAFEGDWYDSTPKDRQLALLKLADRIEEQADEIVKLEAQNVGKPAAVTLS
                     EEIPPIVDNLRFFGGAARTMEGRSAGEYMAGFTSYVRREPIGVAGLIAPWNYPLMMAI
                     WKIGPALATGNAIVLKPSEMTPLTALKLAELTADILPPGLFNVVTGDGVPVGDTLVRH
                     PDVGIVSLTGDTATGKLIAANSAETLKRVHLELGGKAPFIVFDDADMEQVVEWIKIGG
                     YFNSGQDCTASARILVGSGAYENVLAELVPAVESIKVGDPFADDTEMGSMVSKGQLER
                     VVGFVERAEEAGAKVLTGGKAIDQDGFWYSPTIVSDVGQDAEIIQKEVFGPVVTVQRF
                     DDEAQALEWANGVP"
BASE COUNT          413 a          827 c          895 g          399 t
ORIGIN      
        1 cggctccttc cgatggcggt gggagcggtc tgaggacgcg ggtgcgacct ggtcggcgct
       61 ctgggtcatc gaaccggcgg gagtgaagtc gccgctcggg atgagagaaa ggcaaggaac
      121 cagcaggaac ggcaaggaaa cggcacccct cggcaagaac ccgctatccg cggtccgagc
      181 ctgagcctag cgtcggggac gtgctccggt tcatcgctcg ccggctgctc tactccgtcc
      241 tcgtcctcgt catcatctcg atcctcacgt tcctgatctt cgtgaagctg ccggccggcg
      301 atccggcgcg gcgggcggtc ggtcggagga cggatccaca acagatcgcg aacgcgcggg
      361 aagccttcgg cctcgaccga cccttgatcg tccagtacgg caggttcgcg aaggggttga
      421 tcccgctgcc gaacacgttc ctgtccgagg acgtctacta ctcgttctcg aactacctgc
      481 cggtgcgcga ggagatctgg cgtcggctcc ccgtctcggc gatcctcgcg acgggggcgg
      541 ccgtgctctg gctcctgatc gggatcccgc tcgggatcgc atcgggcgtg cgaccccggt
      601 cgctgagcgc ccgatggacg atggtcctgg cgatcgtggg ggtatcgctg cccgtcttct
      661 ggctcggcca gctgctgctg tacgtggtct ggtacaagct ccacctcgcg ccgtcggcgg
      721 gcttggagat caacgccagc gtgtggggga gcgcgctgaa gggcaagttc ctccttccct
      781 ggatcacggt cgcggtcggc tacgcagcga tctacgcccg gatgctccgc agcaacatga
      841 tcgagacgac ccaggaggac tacatgcgca cggcgcgggc caagggtctc tccgagcgcc
      901 gggtcatcta caagcacggc ctccgcgggg ccctgacccc gatcgtgacg atgctcggcg
      961 tcgacttcgc cacgatcctc gcggggctcg tgatcaccga gacgctcttc gggctccccg
     1021 gcctgggcca gctcgccgtc cgctcgatct acagcaacga cttccccatg gtgatgggcg
     1081 tgaccgtcgt ggggtcgatg ttcatcctcg ccgccaacat cgtcgtggac gtcgcgtacg
     1141 cgttcctcga cccccgcgtc cgctactgac cagctgtcga cggaggcgtt ccgcgagagc
     1201 tcatccggcc ggccgacggt cgggatgcgc ctcagcggac ggatgtcgca tactggcgaa
     1261 ccgaacgttg gtgcaggagc cgcgcaggca ggcggccgag cgaggagggg aacccatggc
     1321 ggacgcctat cagatgttca tcgacggcga gtgggtgaac tcgtcggacg gacagacccg
     1381 cgacgtgatc aaccccgcga ccggcgaggt catcgcctcg gtgcaggaag gcacgaagga
     1441 ggacgcgaac agggccgtcg cggccgccag gaaggcgttc gagggggact ggtacgactc
     1501 gacgccgaag gaccggcagc tcgccctgct aaagctcgcg gaccggatcg aggagcaggc
     1561 cgacgagatc gtgaagctcg aggcccagaa cgtcgggaag ccggccgccg tcaccctctc
     1621 cgaggagatc cccccgatcg tcgacaacct ccggttcttc ggaggcgccg cgcggacgat
     1681 ggagggccga agcgccggtg agtacatggc cgggttcacg agctacgtcc gtcgcgagcc
     1741 gatcggcgtg gcggggctga tcgcgccctg gaactacccg ctgatgatgg cgatctggaa
     1801 gatcggaccg gccctcgcga cggggaacgc gatcgtcctc aagccgtccg agatgacgcc
     1861 gctcacggcg ctgaagctcg cggagctgac ggccgacatc ctgccgccgg gcttgttcaa
     1921 cgtcgtcacc ggcgacggcg tccccgtggg cgacacgctc gtccggcacc cggacgtggg
     1981 catcgtgtcg ctcaccggtg acaccgccac cggcaagctg atcgcggcca actccgcgga
     2041 gaccctgaag cgggtgcacc tggagctcgg cggcaaggcg ccgttcatcg tgttcgacga
     2101 cgccgacatg gagcaggtcg tcgagtggat caagatcggt ggctacttca actccggtca
     2161 ggactgcacg gcttcggcgc ggatcctcgt cgggtcgggg gcctacgaga acgtcctcgc
     2221 ggagctggtg ccggcggtgg agtcgatcaa ggtcggcgac ccgttcgcgg acgacaccga
     2281 gatgggctcg atggtctcga aggggcagct cgagcgcgtc gtcgggttcg tggagcgggc
     2341 cgaggaagcc ggcgcgaagg tgctcacggg cggcaaggcg atcgaccagg acgggttctg
     2401 gtactcgccc accatcgtca gcgacgtggg gcaggatgcc gagatcatcc agaaagaggt
     2461 cttcggcccg gtcgtcaccg tccagcgctt cgacgacgag gcgcaggcgc tcgagtgggc
     2521 gaacggcgtg ccgt
//
LOCUS       DASUVE010000339         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1108295, whole genome shotgun sequence.
ACCESSION   DASUVE010000339 DASUVE010000000
VERSION     DASUVE010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108295"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /gene="hflX"
                     /locus_tag="VF036_07340"
     CDS             <1..904
                     /gene="hflX"
                     /locus_tag="VF036_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX6265078.1"
                     /translation="GKAQVELAQLNYLLPRLRGWGEAMSRLGGGIGTRGPGETKMEVD
                     RQHIRRRISKLRRDIKTLTKTRDLKRSRRQDSGIPQVAIAGYTNAGKSTLMNRLTGAR
                     TVVADQLFATLDPTTRRIELPGRRRAVISDTVGFVGKLPHDLVEAFRSTLEEVVLADL
                     IVHVADATSPALDEQVDAVRRVLGEIGAGQIPEVLTLNKIDEVPGSARARLAGRFPGS
                     VAVSALTGEGSDGLLDEIGARVPHPPVEVTLLVPFGREDVTARLYREAEVLSKETDTD
                     GTIVRVRVGLRLLSAIRPFVVDGS"
     gene            complement(908..1903)
                     /locus_tag="VF036_07345"
     CDS             complement(908..1903)
                     /locus_tag="VF036_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX6265079.1"
                     /translation="MSMQSNTKLESQIRSQPDELSHLLTSDRARDQTVVAADRLKRSK
                     RIWLVGTGTSHHAAILGAGMLQDAGRSAVAVSSMRFVVWAPIVGPDDAIILITHTGET
                     AYALAARALATSASLDVMMITKRGTPFPHAVETCPAEAAETYTVSYTTALLVLAMIAK
                     EMGADSITEDTLALVPGAVRNAIEAPGTESVRVPERTLVIAGAGPAAFTAREGALKLR
                     EGARVMAEGHDIEYLLHGSAVPLDHRDALFGLTTPDADGFVRAVLGAAAAEGIPVSTL
                     AEPAPLPMLLAQIPLTVRLQILALRLALERGQDPDTVITGNWADEGLWRLGRPDR"
     gene            complement(1956..2564)
                     /gene="lexA"
                     /locus_tag="VF036_07350"
     CDS             complement(1956..2564)
                     /gene="lexA"
                     /locus_tag="VF036_07350"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX6265080.1"
                     /translation="MSDMTSRQQRILDFIEKTVRERGYPPTVREIGEAVGLTSSSSVH
                     AQLANLERKGLLTKDPSKPRAMTLSDERRAEGTPVPLIGRIAAGEPVLAEEHVEDRIM
                     VPAEFVRGDGHFALRVAGESMVGAGILDGDVVVVRSQRSATDGDIVAALLPGPAEDEA
                     TVKRLGHDGARVMLIPENPALEPFEMLDGRILGKVVAVLRKL"
BASE COUNT          375 a          905 c          933 g          408 t
ORIGIN      
        1 gggcaaggca caggtggagc tcgcccagct gaactacctg ctgccccggc tccgtgggtg
       61 gggtgaggcg atgagccgcc tgggaggcgg gatcggcacg cggggtccgg gcgagacgaa
      121 gatggaggtc gaccgtcagc acatccggcg ccgcatctcg aagctccgcc gtgacatcaa
      181 aacgctcacg aagacccgcg acctcaagcg ttcccgccgc caggattccg ggatcccgca
      241 ggtggcgatc gccgggtaca cgaacgccgg caagtcgacc ctgatgaacc gcctgaccgg
      301 ggcgaggacg gtcgtggccg atcagctctt cgcgacgctc gatcccacca cacgccggat
      361 cgagctcccc ggtcggcgtc gcgccgtgat ctccgacacc gtcgggttcg ttggcaagct
      421 gccccacgac ctggtggagg cattccgctc caccctcgag gaggtggtct tggccgacct
      481 gatcgtgcac gtcgccgacg ccacgtcacc ggcgctcgac gagcaggtgg atgccgtccg
      541 gcgcgtgctg ggagagatcg gggcgggcca gatcccggag gtcctcacgt tgaacaagat
      601 cgacgaggtc cccggatccg ctcgggcgag gctcgccggc cggttcccgg ggtccgtcgc
      661 ggtctccgcg ctcaccggcg agggttctga cggtctcctc gatgagatcg gggcgcgtgt
      721 gccccatccg cccgtcgagg tgacgctcct cgttccgttc ggacgcgagg acgtgacggc
      781 gcgcctctac cgggaggccg aggtcctgtc caaggagacg gataccgacg ggacgatcgt
      841 gcgggtccgc gtgggtctgc ggctgctgtc cgcgatccgt ccgttcgtcg tcgacgggag
      901 ctgagcctta ccggtccggc cggcccagtc tccagagccc ctcgtccgcc cagttgccgg
      961 tgatcaccgt atcggggtcc tggccgcgct cgagcgcgag ccggagcgcg aggatctgca
     1021 gccggacggt gagcgggatc tgggccagga gcatcgggag gggcgccggc tcggccagcg
     1081 tggagaccgg gatcccctcg gcggcggccg cgcccagcac ggcacgcacg aagccgtccg
     1141 catcgggtgt cgtcaggccg aacaatgcgt cccgatgatc gagcggcacg gccgagccgt
     1201 ggaggagata ctcgatgtcg tgcccttccg ccatcacccg ggcgccctca cgcagcttca
     1261 gcgcgccctc acgtgcggtg aacgcggcag gcccggcgcc ggcgatcacg agcgtccgct
     1321 cggggacccg aacagactcg gtcccgggcg cctcgatcgc attgcgcacc gctccgggaa
     1381 cgagcgcgag cgtgtcctcc gtgatcgagt ccgcgcccat ctccttcgcg atcatcgcga
     1441 gcacgagcag cgcggtcgtg tagctcaccg tgtaggtctc cgccgcctcg gccgggcacg
     1501 tctcgacggc gtgcgggaag ggcgtgcctc gcttcgtgat catcatcacg tccaggctgg
     1561 cgctcgtcgc gagcgcgcgg gccgcgagcg cataggccgt ctcacccgtg tgggtgatga
     1621 ggatgatcgc gtcgtcgggg ccgacgatgg gcgcccagac gacgaagcgc atcgacgaca
     1681 cggcgacggc cgaacgcccc gcgtcctgga gcatccctgc tccgaggatc gcggcgtgat
     1741 ggctcgtgcc ggtcccgacg agccagatcc gcttcgatcg cttgaggcga tcggccgcca
     1801 cgacggtctg atcccgggca cggtcagagg tcaggaggtg ggagagctcg tcgggctgcg
     1861 agcggatctg actctccagc ttggtgttcg actgcatgga catcggtcgg gtccccctgg
     1921 cctgtcggat cgtcgggggt ggagcttagc ccgactcaga gcttcctgag caccgccacg
     1981 accttgccga ggatcctgcc gtcgagcatc tcgaagggtt cgagcgccgg gttctcgggg
     2041 atgagcatca cgcgcgcgcc gtcgtgaccc agacgcttca ccgtggcctc gtcctccgcg
     2101 ggtccgggca ggagcgcggc gacgatgtcg ccgtcggtcg ccgatcgctg gctgcgcacg
     2161 accacgacgt cgccgtcgag gatgcccgcg ccgaccatcg attcgccggc gacccgaagc
     2221 gcgaaatgac cgtcgccccg aacgaactcg gccggcacca tgatgcggtc ctcgacgtgc
     2281 tcctccgcga ggacgggctc gccggccgcg atccgtccga tcagcgggac cggtgttccc
     2341 tccgctcgtc gctcgtccga gagcgtcatg gcccggggct tgctcgggtc cttcgtcagg
     2401 aggcccttgc gttcgaggtt ggccagctgg gcgtggacgg acgacgagga cgtgaggccc
     2461 acggcttcgc cgatctcgcg gaccgtcggc gggtagccgc gctcgcgcac ggtcttctcg
     2521 atgaaatcga ggatgcgctg ctgccgcgaa gtcatgtcag acatggtgcg tagcctcctg
     2581 ttccgggcgg cgcgtcttcg agccatctcg ggccagaggc c
//
LOCUS       DASUVE010000340         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_545136, whole genome shotgun sequence.
ACCESSION   DASUVE010000340 DASUVE010000000
VERSION     DASUVE010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545136"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VF036_07355"
     CDS             complement(<1..272)
                     /locus_tag="VF036_07355"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006939923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6265081.1"
                     /translation="MTTERRSERRSELDAKVNQILSRHPAVGLAVGIIRNGELQSFHG
                     HGLADIASHTPITEDTVIRIGSLTKTFTAVAVMQLWEQGLVDLDAAA"
     gene            complement(278..1045)
                     /locus_tag="VF036_07360"
     CDS             complement(278..1045)
                     /locus_tag="VF036_07360"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HEX6265082.1"
                     /translation="MSRTLASGRLEDDLSEPSASGRFSDGELDGMPEPVRRYFRAAIA
                     PGAPLAASARLRMRGRIKLGLWLPFRARQVIAPHRGFVWAAHVAGIISGSDRYIDGHG
                     SMQWQLLGLVPIVRAEGPDISRSSVGRFGAEAIWLPTSLLPRFGVAWTADDDSHVTAR
                     FRVDDVELALHLSLDEGARVRSVQLDRWADPESSGAFAWHPFGIEVHSYATFDGVSIP
                     NRGRGGWFFGTDRWPEGDFFHYEITDLSPREPRGREP"
     gene            1342..1989
                     /locus_tag="VF036_07365"
     CDS             1342..1989
                     /locus_tag="VF036_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6265083.1"
                     /translation="MSIVRVHNFSISLDGFGTGEPQSHDAPFGHAGERLHGWMFATRF
                     WREMVGEPGGTRGLDDAFARLHDRGIGAEIMGAGKFGYPGWHEDREWRGWWGPNPPFH
                     TPVFVLTHHTRPSIEMEGGTTFHFLDVSPADALATARDAAGGQDVRLGGGATVIRDFM
                     AAGLVDHMHTVVVPILLGRGVRLWDGLEGVEEDYQVEATSSPSGVTHVTFTRSGV"
     gene            complement(2007..>2266)
                     /locus_tag="VF036_07370"
     CDS             complement(2007..>2266)
                     /locus_tag="VF036_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX6265084.1"
                     /translation="RYTTVDGELELLPGMRLVPAPGHTRGIQMVVVETGGRPTVVCGD
                     VAVWSGELDEPHTEGQLLVRGLDPELVWLAHEREPWRPPAV"
BASE COUNT          372 a          772 c          782 g          340 t
ORIGIN      
        1 gccgcagcat cgagatcaac aagtccttgt tcccacagct gcatcacggc gaccgcggtg
       61 aacgtcttcg tgagcgatcc gatgcggatg acggtgtctt cggttatcgg cgtgtgcgac
      121 gcgatatcgg cgagtccgtg accgtggaag gactgcagtt cgccgttccg gatgatcccg
      181 acggcaagcc ccacggctgg atggcggctg aggatctgat tgaccttggc atccagctcc
      241 gatcgccgct cggacctacg ttccgtggtc attgcgctca tggctcccgc ccccggggtt
      301 cccgcgggct caggtccgtg atctcgtagt ggaagaagtc gccctcgggc caccggtcgg
      361 tgccgaagaa ccagccaccg cgtccgcggt tcgggatgct cacgccgtcg aacgtcgcgt
      421 agctgtggac ttcgatgccg aacggatgcc atgcgaacgc accggagctc tcaggatcgg
      481 cccaccgatc gagctggacc gaccgaacgc gcgcgccctc gtccagcgac aggtggagcg
      541 ccaactcgac gtcgtcaacg cggaaccgcg cggtgacgtg tgagtcgtcg tcggcggtcc
      601 acgcgacacc gaaccgcggg aggagcgagg tcggcaacca gatggcttcc gcgccgaacc
      661 gcccgaccga actgcgggag atgtcgggtc cttccgcgcg cacgatcggg accagcccga
      721 ggagctgcca ctgcatggag ccgtgaccgt cgatgtatcg gtcggagccg ctgatgatcc
      781 cggcgacgtg cgccgcccag acgaaaccac gatgcggcgc gatcacctgg cgcgcgcgga
      841 aggggagcca gagaccgagc ttgatcctgc cgcgcatgcg caggcgcgcg gacgctgcca
      901 gcggcgcacc gggcgcgatc gcggctcgga agtagcggcg cacgggctcg ggcatgccgt
      961 cgagctctcc gtcggagaat cgacccgaag ctgacggttc gctgaggtcg tcctccaacc
     1021 gacccgatgc cagagttcgg ctcatcgcca acacctccga tggcgatcgt tgatcccgca
     1081 gcgtcagcat ggtcgaccgg cgagcgagcg aacacggtcg tccggaccgg cttgggaggg
     1141 ccatctgtcc ccgggacgct cagccgatgc aagaatgggc gcgaaccgtg tgatcagggc
     1201 ggcgtgagaa caccccccac caccactgaa cccgttcggc gtggcactcg gtgtcgtcca
     1261 ccgtcgcgag ttccggtcga ggtctacgat gcgaacgcaa ccgagtggaa cgtgaacctc
     1321 gaagccgaag gagcagcaac catgtcgatc gtccgcgtcc acaacttctc catctcgctc
     1381 gacggtttcg ggaccggcga accgcagagc cacgacgcac cgttcggtca tgccggcgaa
     1441 cggctgcacg gatggatgtt cgcgacccgg ttctggcgcg agatggtcgg cgagcccggc
     1501 ggcacccggg ggctcgacga cgccttcgcg cggctgcacg accgggggat cggcgccgag
     1561 atcatgggcg ccggtaagtt cggttacccc ggatggcacg aggaccggga gtggaggggc
     1621 tggtggggtc ccaacccgcc gttccacacg ccggtcttcg tcctcaccca tcacacgcgt
     1681 ccgtccatcg agatggaggg cgggacgacg ttccacttcc tcgacgtgtc gcccgccgac
     1741 gcgctcgcga cggctcggga cgccgcgggc ggccaggacg tgcgcctcgg gggaggcgcc
     1801 accgtgatcc gtgacttcat ggccgccggg ctcgtggatc acatgcacac cgtcgtcgtc
     1861 ccgatcctgc tcggccgtgg cgttcggctc tgggacggac tggagggcgt cgaggaggac
     1921 taccaggtcg aagccacctc ctcgcccagc ggggtcacac acgtgacgtt cacccgttcg
     1981 ggcgtctagc gggagaggct cgtacctcag acggcgggag gtcgccaggg ctcgcgctcg
     2041 tgcgcgagcc agaccagctc ggggtcgagg ccgcgcacca gcagctggcc ttcggtgtgc
     2101 ggctcgtcga gctcgccgga ccacaccgcc acgtcgccgc agacgacggt cggacgccca
     2161 ccggtctcga ccacgaccat ctggatgcca cgggtgtggc cgggcgccgg cacgagccgc
     2221 atcccgggga gcagctcgag ttcgccgtcg accgtcgtgt agcgca
//
LOCUS       DASUVE010000341         5728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1299903, whole genome shotgun sequence.
ACCESSION   DASUVE010000341 DASUVE010000000
VERSION     DASUVE010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299903"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1174
                     /locus_tag="VF036_07375"
     CDS             80..1174
                     /locus_tag="VF036_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX6265085.1"
                     /translation="MAHRITLIPGDGIGPEVVEAARRVVEATGVPVEWDRQDMGAGAF
                     SRTGDPLPVATLDSLRSTRVALKGPIGTPVTSGLRSTNMALRQGLDLYANVRPCRRYP
                     GVPSVYGDVDIVVVRENTEGMYTGIEIEMGTPAATELIGFIHDRTGRTVRPDSGISVK
                     DISEFGATRVVRFAFGWAAGHGRATVTAAHKANIMKFTDGLFLEAARRVAAEHPGIAF
                     EDRIIDALCMQLIGSPDRFDVLVMPNMYGDLVAELCAGLIGGTGVAPGAHFGDDLAVF
                     EATHGTAGHLAGTNRADPIGVILSAAMLLRHVGEREAGDRIEAAVVDLLADGRDVTYD
                     LRAPEDDRPAAGTFQVADAVVARVAARPVR"
     gene            1209..2132
                     /gene="mdh"
                     /locus_tag="VF036_07380"
     CDS             1209..2132
                     /gene="mdh"
                     /locus_tag="VF036_07380"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX6265086.1"
                     /translation="MKITVVGSGFVGQTAAMRLLERGMGQVVLIDIIEGKPQGLALDL
                     RQAAPIEGYEPSIVGTNDYADTAGSDIVVITAGFPRQPGMSRMDLLGKNAAIMRDVVS
                     RVVPGSPDAILIVVSNPLDEMTFLAADASGFPRERVTGMAGVLDSARLRAFIAEELSV
                     SPSAVAAITLGSHGDAMVALPRHATVNGTPLPELVDDATLDRLFQRTRDGGAEIVALL
                     KSGSAYYAPSASVVAMVEAIARDAKDVLPVCAWTTGEYGISDVYVGVPARLGSKGVEE
                     IVELDLNEDELARLRAAAEGIRAKCADLASL"
     gene            complement(2129..2668)
                     /locus_tag="VF036_07385"
     CDS             complement(2129..2668)
                     /locus_tag="VF036_07385"
                     /inference="COORDINATES: protein motif:HMM:NF021079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trp biosynthesis-associated membrane protein"
                     /protein_id="HEX6265087.1"
                     /translation="MRDERWPTAAGFALTIAGAVLAAVATTTTWIATGLRRDVRGILD
                     LEFRGLDLVEGIVALVVAAVALIGLVLVRRFRGSARVGIAVGVVACGLVLVALPVWVA
                     VRAEDRAVDEIARVVAASSGSTIEEATERVRTDPDLAVRSETTVWLPIVSGLLVVLGA
                     GATLTSSVRTAREAHGAPV"
     gene            2720..2887
                     /locus_tag="VF036_07390"
     CDS             2720..2887
                     /locus_tag="VF036_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265088.1"
                     /translation="MAPFLRPLREHVGNFFTNWSESDLPVARKVALTVRNRARSLVNK
                     GCCGHHGEPGC"
     gene            complement(2929..3999)
                     /locus_tag="VF036_07395"
     CDS             complement(2929..3999)
                     /locus_tag="VF036_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX6265089.1"
                     /translation="MTGRHRVTLIPGDGIGPEVSSAARMVLDAAVPGIEWDERTAGAA
                     ALTEHRDLLPEETLDAIRTTRVAIKGPITTPVGSGFRSINVALRQGLDLFAAVRPARS
                     LPGVSTRHEKVDLVVVRENTEDLYQGVEFAAGSPQADALRIELRSLAGVEIKPDAGVT
                     VKPSSVAGTRRIVRFAFEYAKTNRRARITLGHKAGIMPTSDGLFLTTARNEEIDRGEV
                     GFEDMQIDHLTARLVRDPARFDVLLLPNLYGDILSDLCAGLVGGLGLVPGANIGWEYA
                     VFEPVHGSAPDIAGTGVANPIAMILSGAMLLRHLGEMTGASAVEAAVDSVLSDGTVRT
                     PDLGGSSSTTQVTHAILRALTA"
     gene            complement(4070..4837)
                     /locus_tag="VF036_07400"
     CDS             complement(4070..4837)
                     /locus_tag="VF036_07400"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX6265090.1"
                     /translation="MAASLTPAEILNSSDERRVGATPLVRLTLRIRRFNPQVSEEPWW
                     DEFTIQVDPYERLVEALHQVKWHHDGTLTFRRSCAHGICGSDAMVINGRNALACKILV
                     KDVAPKVTIEPIRGLAVLKDLIVDMEPFFDGYRAVLPYLVNDEETERERVQMPEERER
                     FDDTTKCILCAACTTSCPIFWSDETYIGPAAIVNAHRFIFDSRDRAPRERLGVLSEKT
                     GVFRCRTTFNCTDACPRGIEVTKAIQEVKRAVLFDRR"
     gene            complement(4837..>5728)
                     /locus_tag="VF036_07405"
     CDS             complement(4837..>5728)
                     /locus_tag="VF036_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6265091.1"
                     /translation="RDVVSRSIYQEIKEGRGLGPRGDYVLLDLTHLDPRVLEEKLPDI
                     TEFARVYLQVEPTTEPVPIQPTAHYAMGGIPTDVHARVVTGSEETPVPGLYAAGECAC
                     VSVHGANRLGTNSLLDIVVFGRRGGDDMARHCEATDLPALPGEATAHTLDLLDGILTR
                     QTGDNVADIRAELQDSMFDLAFVVRTEESLTEMAGIVGSLHERYQRIAITDKGTVYNT
                     DLMEAVEVGFLLDCAATLVAAAAARDESRGAHYREDHPLRDDANWLKHSLAYRNDDGS
                     IRLDSKPVQMGPYVPMERKY"
BASE COUNT          853 a         2028 c         2001 g          846 t
ORIGIN      
        1 caggcccttc tcggtcatgg ccccaggata gccggggtgc gcgacgcggc ccaaaccggg
       61 gtcggtaccc ttggtcccga tggcgcaccg catcacgttg atccccggcg acggcatcgg
      121 ccccgaggtg gtcgaggccg cgcgccgcgt cgtcgaagcc acgggcgtcc cggtcgagtg
      181 ggaccgtcag gacatgggcg ccggcgcctt ctcgcggacc ggcgacccgc tccccgtcgc
      241 gaccctcgat tcgctccggt cgacacgggt ggccctgaag ggtccgatcg ggacccccgt
      301 gacctcgggc ctgcgcagca cgaacatggc gctccgccag ggcctcgacc tgtacgccaa
      361 cgtccggccc tgccggcgct acccgggggt tccgtcggtc tacggcgacg tcgacatcgt
      421 cgtcgtccgc gagaacaccg aggggatgta caccggcatc gagatcgaga tgggcacccc
      481 ggcggcgacc gagctgatcg ggttcatcca cgaccgcacc ggacggaccg tgcggcccga
      541 ctccgggatc tcggtcaaag atatctccga gttcggcgcc acgcgggtag tgcggttcgc
      601 gttcggctgg gccgccggcc acggccgcgc caccgtcacc gcggcgcaca aggcgaacat
      661 catgaagttc accgacgggc tgttcctcga ggccgcccgg cgcgtggcgg ccgagcaccc
      721 ggggatcgcg ttcgaggacc ggatcatcga cgccctgtgc atgcagctga tcggatcgcc
      781 cgaccggttc gacgtcctcg tgatgccgaa catgtacggc gacctcgtcg ccgagctgtg
      841 cgccggcctg atcggcggga ccggcgtggc gcccggcgcg cacttcgggg acgacctcgc
      901 cgtcttcgag gccacccacg gcaccgccgg gcacctggcg gggacgaacc gcgcggaccc
      961 gatcggggtg atcctgtcgg cggcgatgct cctgcggcac gtgggggagc gcgaggcggg
     1021 ggaccggatc gaggccgccg tggtcgacct gctcgccgac gggcgcgacg tcacctacga
     1081 cctgcgcgct cccgaggacg accgccccgc ggcggggacc ttccaggtcg cggacgccgt
     1141 ggtcgctcgc gtggcggccc gacccgttcg atagcctggc cgcccacatc gatccgagga
     1201 gcaccgtcat gaagatcacc gtcgtcggca gcgggttcgt cgggcagacc gccgcgatgc
     1261 gtctgctcga gcggggcatg ggccaggtcg tcctgatcga catcatcgag ggcaagccgc
     1321 aggggctcgc cctcgatctc cgacaagcgg cgccgataga gggctacgag ccctcgatcg
     1381 tgggaacgaa cgactacgcg gacacggccg gctcggacat cgtcgtgatc accgccggct
     1441 tccctcggca gccggggatg agcaggatgg atctcctcgg gaagaacgcc gcgatcatgc
     1501 gggacgtcgt ctcccgggtg gtacccgggt cgcccgacgc gatcctgatc gtcgtatcga
     1561 acccgctcga cgagatgacg ttcctcgcgg cggacgcctc cggcttcccc cgcgagcggg
     1621 tcacgggcat ggccggggtg ctggactcgg cccgcctgcg cgccttcatc gcggaggagc
     1681 tatcggtctc accgagcgcg gtcgcggcga tcaccctggg ctcgcacggg gatgcgatgg
     1741 tggccctccc acgtcacgcc accgtgaacg gcacgcccct gccggagctg gtcgacgatg
     1801 ccaccctgga tcgtctcttc caacggacgc gcgacggggg agccgagatc gtggcactcc
     1861 tgaagtcggg gtcggcctac tacgcgccga gtgcctcggt ggtcgcgatg gtcgaggcga
     1921 tcgcgcgtga cgcgaaggac gtcctcccgg tctgcgcctg gacgaccggc gagtacggca
     1981 tcagcgacgt gtacgtgggc gtccccgccc gactgggcag taagggggtc gaggagatcg
     2041 tcgagctcga cctgaacgag gacgagctcg cgaggctccg ggcggccgcc gagggtatcc
     2101 gcgcgaaatg cgcggacctc gcctcgctct agaccggagc tccgtgcgcc tcgcgcgcgg
     2161 tgcgcacgct cgaggtcaag gtcgctccgg ccccgagcac gaccagcagc cccgagacga
     2221 tcggcagcca cacggtcgtt tctgaccgga ccgcgaggtc ggggtcggtg cgtacccgtt
     2281 cggtcgcctc ctcgatcgtg ctgcctgagg acgccgcgac gacccgggcg atctcatcga
     2341 cggctcggtc ctccgcccgg accgcgaccc acaccggaag ggccacgagc acgaggccgc
     2401 aggcgacgac gcccacggcg atcccgacgc gggcgctgcc gcggaaccgg cggacgagca
     2461 cgaggccgat cagcgcgacc gccgcgacca ccagcgcaac gatgccctcg acgagatcga
     2521 gaccacggaa ctcgaggtcc aggatccctc gaacgtcccg gcgcaggccc gtcgcgatcc
     2581 atgtggtggt cgtcgccacg gcggcgagga ccgccccggc gatcgtcagt gcgaagccag
     2641 ccgccgtggg ccaccgctcg tcccgcacgg aaggagtctc gcatcaggcg acccgcggac
     2701 caaggactac gatgccgcga tggccccgtt cctgcgtccg ctgcgcgagc atgtcggcaa
     2761 cttcttcacc aactggtccg agtccgatct cccggtcgcg cggaaggtgg cgctcaccgt
     2821 ccggaacaga gcgaggtccc tcgtgaacaa ggggtgttgc gggcaccacg gcgaaccggg
     2881 ctgctgacgc aaaaccccgg accccggtcg gggtccgcac caccacagct aggcggtgag
     2941 tgcccgcagg atcgcgtgcg tgacctgcgt cgtggacgag gagccgccga ggtccggcgt
     3001 ccgcaccgtg ccgtccgaga ggacggagtc cacggccgcc tccacggcgg acgcgccggt
     3061 catctcgccg agatggcgga ggagcatcgc gcccgaaagg atcatcgcga tcgggttcgc
     3121 caccccggtg ccggcgatgt ccggcgcgct cccgtggaca ggttcgaaga ccgcgtactc
     3181 ccagccgatg ttcgccccgg ggaccaaccc cagtccgccg accagccccg cacagaggtc
     3241 cgagaggatg tcgccgtaga ggttcgggag gagcagcacg tcgaagcgag ccgggtcgcg
     3301 gacgaggcga gcggtcaggt gatcgatctg catgtcctcg aaccccacct cgccgcggtc
     3361 gatctcctcg ttccgggcgg tggtcaggaa caacccgtcc gaggtgggca tgatgccggc
     3421 cttgtgcccc agcgtgatcc gggctcggcg gttcgtcttc gcgtactcga aggcgaaccg
     3481 cacgatgcgt cgcgtccctg cgacggagct cggcttcacc gtgacgcccg cgtccggctt
     3541 gatctcgacc ccggcgaggc tccggagctc gatccggagg gcgtcggctt ggggggaacc
     3601 ggccgcgaac tcgacgccct ggtagagatc ctcggtgttc tcccggacca cgacgagatc
     3661 caccttctcg tgccgcgtcg agacgcccgg caacgaccgg gcgggacgaa cggccgcgaa
     3721 gaggtccaga ccctggcgca gcgcgacgtt gatgctgcgg aaccccgagc ccaccggcgt
     3781 cgtgatcggg cccttgatcg cgacgcgcgt ggtgcggatc gcgtcgaggg tctcctccgg
     3841 caggaggtcc ctgtgctcgg tgagggcggc tgcgcccgcc gtccgctcgt cccactcgat
     3901 cccgggcacc gccgcatcga gcaccatccg cgcggccgag gacacttcgg gtccgatccc
     3961 atcgccgggg atcagcgtga cgcgatggcg tccggtcatc gaggcaccct atcgacgatc
     4021 tcccgtcgcc cgtctcgccg ggatccgtcc tcggtgagcg agaccgacgt cagcgtcggt
     4081 cgaagaggac ggcgcgcttg acctcttgga tggccttcgt gacctcgatg ccgcgtgggc
     4141 acgcgtcggt gcagttgaac gtcgtccggc agcggaacac gccggtcttc tcggacagga
     4201 cgccgagtcg ctcgcgcggg gcgcgatcgc gtgagtcgaa gatgaaccgg tgcgcgttca
     4261 cgatcgcggc cggtccgatg taggtctcgt cggaccagaa gatcgggcag gacgtggtgc
     4321 acgcggcgca caggatgcac ttcgtcgtgt cgtcgaagcg ctcccgctcc tccggcatct
     4381 gcacgcgctc ccgctccgtc tcctcgtcgt tcacgaggta ggggagcacc gcgcgatacc
     4441 cgtcgaagaa gggctccatg tccacgatca ggtccttcag gacggcgaga ccgcggatgg
     4501 gctcgatggt caccttcgga gcgacgtcct tgaccaggat cttgcaggcg agggcgttcc
     4561 gcccgttgat caccatcgcg tccgagccgc agataccgtg tgcgcacgaa cgccggaacg
     4621 tgagggtccc gtcgtgatgc cacttcacct gatggagggc ctcgacgagt cgttcgtacg
     4681 gatccacctg gatcgtgaac tcgtcccacc acggctcctc cgagacctgc gggttgaacc
     4741 ggcggatcct gagcgtgagc ctcacgagcg gggtcgcgcc cacccggcgc tcgtccgatg
     4801 agttcaggat ctcggccggc gtgagggatg ccgccatcag tacttccgct ccatgggtac
     4861 gtacgggccc atctgcaccg gcttcgaatc caggcggatc gagccgtcgt cgttccggta
     4921 cgcgagcgag tgcttcagcc agttggcgtc gtcgcgcagc gggtgatcct cccggtagtg
     4981 ggctccgcgg ctctcgtcgc gcgccgccgc cgcggcgacg agggtcgcgg cgcaatccag
     5041 caggaacccc acctcgaccg cctccatcag gtccgtgttg tagacggtgc ccttgtcggt
     5101 gatcgcgatc cgctggtacc gctcgtggag cgaaccgacg atcccggcca tctcggtgag
     5161 gctctcctcg gtgcggacca cgaacgccag gtcgaacatc gagtcctgga gctccgcccg
     5221 gatgtcggcc acgttgtcgc ccgtctgccg cgtgaggatg ccgtccaaca ggtcgagggt
     5281 atgcgcggta gcctcgcccg gcagcgccgg gaggtcggtc gcctcgcaat gccgcgccat
     5341 gtcgtccccg ccccgccggc cgaagacgac gatgtccagc agggagttcg tccccagccg
     5401 gttcgcaccg tggaccgaga cgcaggcgca ttcgccggcc gcgtacaggc ccggcaccgg
     5461 cgtctcctcg gacccggtca ccacgcgcgc gtgcacgtcg gtcgggatcc cgcccatcgc
     5521 gtagtgggcg gtgggttgga tcgggacggg ctccgtcgtg ggctcgacct ggaggtacac
     5581 ccgcgcgaac tccgtgatgt ccgggagctt ctcctcgagg acccgcggat ccaggtgcgt
     5641 caggtcgagg aggacgtagt cgcctcgcgg cccgagcccc cggccctcct tgatctcctg
     5701 gtagatcgac cgggagacga cgtcgcgg
//
LOCUS       DASUVE010000342         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1110385, whole genome shotgun sequence.
ACCESSION   DASUVE010000342 DASUVE010000000
VERSION     DASUVE010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110385"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1583
                     /gene="glmU"
                     /locus_tag="VF036_07410"
     CDS             162..1583
                     /gene="glmU"
                     /locus_tag="VF036_07410"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX6265092.1"
                     /translation="MGTTPKRTPRSLAAVVLAAGQGKRLRSDLPKVLHPVCGRPILWH
                     TLQVVRAAKPSRIVVVVGHGADDVRDAVESWGIEPAPVFVEQAEQLGTGHAVQLAEDA
                     VGKVADVLVANGDFDPVTVADVRKLLSSHRRSGAAVTLGSTVLDAPGGYGRVVRDGRR
                     VVDVVEGRDAPPAVRKIHEVATNWIAFRRDLLFATLPLLDRENRQREYYLNRAISILL
                     DKGERVDAVVCDTGRVLGANTRAGLAGLEALIRRRTNDFHMAAGVRLIDPSATYIDVE
                     VRLGKDTVVYPNTFLETGVRIGTGCRIGPSTTIARSRVGDRSVVWFSVLEDARVGKDC
                     EVGPFARLRPGAVLEDGALVGNFVEVKGSRIGTGSKAKHLTYIGNAELGVGVNVGAGT
                     VVVNYDGYDKHPTVIGDGARIGSDTMLVAPLTVGKGAVTGAGSVITKDVPAGALAVER
                     SDQKNVKGYRKRADAKHRSKGDH"
     gene            1617..>2103
                     /gene="prs"
                     /locus_tag="VF036_07415"
     CDS             1617..>2103
                     /gene="prs"
                     /locus_tag="VF036_07415"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX6265093.1"
                     /translation="MMLFSGTIHPALGSEIAECLGIPVSHSELRRFASGEIYFRAEES
                     VRGADVFVVQSHYEPVNEALMEQLIMIDAMKRASAKRINAVIPFYGYSRQDHKALSRE
                     PISAKLVADLLSTAGADRVISVDLHSGQIQGYFDFPFDHLTALPILSDYFKDELGLHG
                     DN"
BASE COUNT          326 a          717 c          732 g          328 t
ORIGIN      
        1 ctcacccagc tcggcttccc ggccccggtc gccgaggaga tgtcggagat gcacacgccg
       61 aacttcatgt agcgatccgc ggacggatct cagcgagcca cgcgcgcggc ggtatgggac
      121 catggcctcg gccttcccgc ccccgacgct gaaggactga cgtgggcacg acacccaagc
      181 gcaccccgcg atcgctcgcc gccgtggtcc tcgctgccgg ccaggggaag cggctgcggt
      241 cggacctccc caaggtgctc cacccggtct gtggccgtcc gatcctgtgg cacaccctcc
      301 aggtcgtgcg cgccgcgaag ccctcgagga tcgtcgtcgt cgtcggtcac ggcgcggacg
      361 acgtccgcga cgccgtcgag tcctggggta tcgagcccgc gcccgtcttc gtcgagcagg
      421 ccgagcagct cgggaccggt catgccgtcc agctcgccga ggacgccgtc ggcaaggtgg
      481 cggacgtcct cgtggcgaac ggggacttcg atccggtcac cgtggctgac gtgcggaagc
      541 tcctgtcgtc gcatcggcgc tccggcgcgg cagtcaccct cggctccacg gtcctcgacg
      601 cccccggcgg ctacgggagg gtcgtccgcg acggtcggcg cgtcgtcgac gtggtcgagg
      661 ggcgcgatgc gccgcccgcc gtccggaaga tccacgaggt cgcgacgaac tggatcgcgt
      721 tccgccggga tctgctgttc gcgaccctgc cgctcctcga ccgggagaac cgccaacgcg
      781 agtactacct gaaccgggcg atctcgatcc tcctcgacaa gggcgagcgc gtcgacgccg
      841 tcgtctgcga tacgggcagg gtactcggcg ccaacacccg cgccgggctc gccggtctcg
      901 aggcgctgat ccgtcgccgg accaacgact tccatatggc cgccggggtc cgcttgatcg
      961 atccctccgc cacctacatc gacgtcgagg tgcgtctcgg caaggacacg gtcgtctacc
     1021 cgaacacgtt cttggagacg ggggttcgga tcggcaccgg ctgcaggatc ggtccgtcca
     1081 ccacgatcgc ccgctcccgg gtcggtgacc gctcggtcgt ttggttctcg gtcctcgagg
     1141 acgcccgggt cggcaaggac tgcgaggtcg gtccgttcgc gcgtctacgg cccggggcgg
     1201 tcctggagga cggtgccctc gtcggcaact tcgtggaggt gaagggctcg cggatcggta
     1261 cgggctcgaa ggccaagcac ctcacgtaca tcggcaacgc cgagctcggg gtgggggtga
     1321 acgtcggggc cgggaccgtc gttgtgaact acgacggcta cgacaagcac cccaccgtga
     1381 tcggcgacgg ggcgaggatc gggtcggata cgatgctggt ggcgccgctg acggtcggca
     1441 agggcgccgt cacgggggcc ggatcggtga tcacgaagga cgtgccggcg ggcgcgctcg
     1501 ccgttgagcg gtccgaccag aagaacgtga agggctaccg gaagcgcgcc gacgccaagc
     1561 atcggtcgaa gggcgatcat tagggggcga ccgtggagat catcaccaag aagcggatga
     1621 tgctgttctc cggaacgatc catccggcgc tggggagcga gatcgccgag tgcctcggca
     1681 tcccggtgag ccacagcgag ctccggcgct tcgcgtcggg ggagatctac ttccgtgccg
     1741 aggagagcgt ccggggggcc gacgtgttcg tggtgcagtc gcactacgag cctgtgaacg
     1801 aagctctgat ggagcagctg atcatgatcg acgccatgaa gcgggcgtcg gccaagcgga
     1861 tcaatgccgt gatcccgttc tacggctact cccgtcagga ccacaaggcc ctgtcccgcg
     1921 agccgatcag cgcgaagctc gtcgccgatc tcctctccac cgcgggcgcc gaccgggtga
     1981 tctcggtcga cctgcattcg gggcagatcc agggctactt cgacttcccg ttcgaccacc
     2041 tcacggcgct cccgatcctg tcggactact tcaaggacga gctggggctg cacggcgaca
     2101 acc
//
LOCUS       DASUVE010000343         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_357517, whole genome shotgun sequence.
ACCESSION   DASUVE010000343 DASUVE010000000
VERSION     DASUVE010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357517"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1153)
                     /locus_tag="VF036_07420"
     CDS             complement(23..1153)
                     /locus_tag="VF036_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6265094.1"
                     /translation="MADSEGPGEVGTSFATPGRPEIGSALAGTPNSQIRDSADLGHDI
                     IATAVPIQEEGEIAGAVRITKNVQQVNDNVRNVTLGLISIGVAGLAAGLLLAFGLAGS
                     LSRPLTRLAKTAKELGEGDLSARTGDVRGPREIEELGRSFDEMAQRVERSVQAQREFV
                     ANASHQLRTPLTAMKLQIEGAISDATDDDVRERLLAADREVDRLSEIVDRLLVMAREI
                     EEGASARVDVEDAARRAVGRWTERAEQRRSSLEARGEGGSARADPTDLDQIIDNLIDN
                     ATSYAPGEVVVETERSNGRVIVAVRDRGDGISPEEVARVTERFYRGREAPAGGSGLGL
                     AIARQLAEKWGGTLTVQSTPGEGTRVEVALQPLPDDVSFTSS"
     gene            complement(1427..>2016)
                     /locus_tag="VF036_07425"
     CDS             complement(1427..>2016)
                     /locus_tag="VF036_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003991734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6265095.1"
                     /translation="LYRAREALARETPDLILLDVMLPDGDGRDLARDIRKDSDVPIVM
                     LTARGEEIDRVLGLELGADDYVVKPFSARELTARIRAIMRRGRRAGPARPVEIGQVRL
                     DPTSRTLTKAGEPVELAAKEFDLLAFLMSHPGEVLRREQIMDEVWDPHWFGPTKTLDV
                     HISWLRKKIEDDPSDPRYITTIRGVGFRFLAPDEA"
BASE COUNT          275 a          709 c          703 g          329 t
ORIGIN      
        1 atcgagcgta cggatggggt ggctaagagg aggtaaagct cacgtcgtcg gggaggggct
       61 ggagcgcgac ctcgacccgc gtcccctcgc cgggcgtgct ctgcacggtg agcgtgccgc
      121 cccacttctc cgcgagttga cgggcgatcg cgagtccgag tccggagcca ccggccggcg
      181 cctctcggcc ccggtagaag cgctcggtga cccgggcgac ctcctcgggg gagatgccgt
      241 ccccccggtc ccgaacggcc acgatgaccc ggccgttcga gcgctcggtc tccacgacca
      301 cctcgcccgg tgcgtaggac gtcgcgttgt cgatcaggtt gtcgatgatc tggtctaggt
      361 cggtgggatc ggctcgggcg gatccgcctt ccccccgggc ctcgagcgac gatcgacgct
      421 gctccgcccg ctcggtccat cggccgaccg cgcgtcgggc ggcgtcctcg acgtccacac
      481 gtgcggacgc gccctcctcg atctcgcgcg ccatcacgag caggcggtcg acgatctccg
      541 agagccggtc gacctcccgg tcggcggcga gaagccgctc gcggacgtcg tcgtcggtgg
      601 cgtcggagat cgcgccttcg atctggagct tcatcgccgt gagcggcgtc cggagctggt
      661 gcgaggcgtt cgcgacgaac tcgcgctggg cctggaccga acgctcgacc cgctgcgcca
      721 tctcgtcgaa cgatcgtccg agctcttcga tctcgcgcgg gccgcggacg tcacccgtgc
      781 gcgccgagag atccccctcg ccgagctcct tggcggtctt cgcgagcctc gtgagcggtc
      841 tcgagagcga cccagcgagc ccgaacgcca ggaggaggcc ggccgcgagt cccgcgaccc
      901 cgatcgagat gaggccgagc gtgacgttcc ggacgttgtc gttcacctgc tggacgttct
      961 tggtgatacg cactgcaccg gcgatctcgc cctcctcctg gatcgggacc gccgtcgcga
     1021 tgatgtcgtg tcccagatcg gcgctgtccc ggatctggga gttcggcgtt ccggcgagcg
     1081 cggagccgat ctcgggccgc cccggcgtcg cgaacgacgt gccgacctcc cccgggccct
     1141 ccgaatcggc cacgagggtc ccgtcccggt ccacgacgat cacgcgcccg ccgacctgct
     1201 gtgcggcctc ggagacgatg gggtcgatcc ggaccgggtc gtcgagctcg ccggggtcga
     1261 ccgccgccgc gatgccttgc gcctggatca gcgcctgggt ctcgagctcc gcggccgccc
     1321 gccgctggag gttgatcgtg agcgggatgc cgagcgcgat gatgaggacg atcaggacgt
     1381 aggcgaaggc gaggacgaga cgggttcgga ccttcgtggc tgcctcctat gcctcatcgg
     1441 gcgccaggaa ccggaacccg acgccccgga tcgtcgtgat gtacctcgga tccgacggat
     1501 cgtcctcgat cttcttgcgg agccaggaga tgtgcacgtc gagcgtcttc gtcgggccga
     1561 accagtgggg gtcccacacc tcgtccatga tctgctcgcg acgcaggact tcgccggggt
     1621 gcgacatgag gaacgcaagg aggtcgaact ccttcgccgc gagctcgacc ggctctccgg
     1681 ccttcgtcag cgtacgggat gtcgggtcga gtctgacctg gccgatctcg acgggtcgag
     1741 cgggcccggc cctgcgtccg cgacgcatga tcgccctgat ccgggcggtg agctcccgtg
     1801 ccgagaaggg cttgaccacg tagtcgtcgg cacccagctc gagtccgagg acccggtcga
     1861 tctcctcgcc tcgggcggtg agcatcacga tgggcacatc ggaatccttc cggatgtctc
     1921 gagcgagatc ccgcccgtct ccgtcgggca gcatcacgtc gagcaggatc aggtccggtg
     1981 tctccctcgc gagcgcctcg cgcgcccggt agaggg
//
LOCUS       DASUVE010000344         3305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_547624, whole genome shotgun sequence.
ACCESSION   DASUVE010000344 DASUVE010000000
VERSION     DASUVE010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547624"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="VF036_07430"
     CDS             complement(<1..1252)
                     /locus_tag="VF036_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6265096.1"
                     /translation="MVERPEPVRDLDWDPKRARAFTDGVADIYEELLTRLRDLPVSRG
                     WGVQEVRDAVAIPVPEEPMPADEILEYLRTMTFDYSGYLGHPRFYAYISGAGTVPGAA
                     ADLLASGLNMNTGGWRLAPSGTEIELAFTRWFAQEIFGLPVGSGGALTSGGAMANFIA
                     LKVARDAKAGWDIRKEGVGAGPTLGLYVSTETHIVSIRGADMLGLGSGNVRKVAVDDG
                     YRIRVDELRAAIAEDRAAGVHPIAVVANAGTVSTGVVDPLEEIADVCRDEDLWFHVDA
                     AYGGPAMLADDLRPLFSGIERADSIAFDPHKWLYTPQSGGCVVVRDLELMRRAFDVDY
                     VAYVVKDEEHTDWGVDLGRHSPNFSRGFWALKVWVSLLAHGRTAYGKRISHDAALARY
                     LAERAEEIDEFELMTPVGLSITCFR"
     gene            complement(1252..1977)
                     /locus_tag="VF036_07435"
     CDS             complement(1252..1977)
                     /locus_tag="VF036_07435"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX6265097.1"
                     /translation="MIDVADLGTILGVWAHPDDDIYLSAGLMAAAVEAGQRVVDVTAT
                     RGEGGSMDEERWPPESMGDVRTNELLRSLDVLGVTEHHFLEGPVDIDMETGLDPAGAE
                     QVRTLMKEIDPDTILTFGPEGMTGHVAHQDVSRWSGEAFAQVAKAGARLYQAVFPTSM
                     ADEWLEKLAPFNIFLPGTPNVVPDEEIDLYFELPPANLDQKIASIKEHASQIEALYEV
                     FGDEGFRRFMAKEGFVLAATKEA"
     gene            complement(2026..2274)
                     /gene="rsrA"
                     /locus_tag="VF036_07440"
     CDS             complement(2026..2274)
                     /gene="rsrA"
                     /locus_tag="VF036_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HEX6265098.1"
                     /translation="MGEKGDMGCDETLREVERYVDGECLRELTVQVEAHLAGCSDCND
                     HAEFKKHMKLLIATKCRGEVLPEGLAERIRERIRTASP"
     gene            complement(2267..2860)
                     /locus_tag="VF036_07445"
     CDS             complement(2267..2860)
                     /locus_tag="VF036_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6265099.1"
                     /translation="MANASRVPAPADQDLLDRFDRDVLPLLPGLYGAALRMTRNPADA
                     EDLLQETTLRAYRGFASFREGTNLKAWLYRILTNSFINIYRKKQREPKTVEGPDDIDE
                     WFLFDRLGAKSVERSAEEDVLEKIPDADVKEVLESIPENFRMAVLLADVEGFSYKEIA
                     EITDVPIGTVMSRLHRGRKALERALYGVAKERGLVDG"
     gene            3022..>3305
                     /locus_tag="VF036_07450"
     CDS             3022..>3305
                     /locus_tag="VF036_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265100.1"
                     /translation="MLVVAGSLVLFNAFLTQAASTPVPFSEFRRAIDNGDLVKEEPVT
                     ITTTTISGVVRTPDGDETYVATIPPGYVPTDLVDDLSARGYEVDGQQPNAL"
BASE COUNT          522 a         1167 c         1068 g          548 t
ORIGIN      
        1 accggaaaca cgtgatcgac agcccgaccg gcgtcatcag ctcgaactcg tcgatctcct
       61 ccgcgcgctc ggccaggtac cgggcgagcg ccgcgtcgtg cgagatccgc ttgccgtacg
      121 cggttcgtcc gtgcgcgagc agcgagaccc acaccttcaa tgcccagaac ccccggctga
      181 agttgggcga gtgccgaccg aggtcgacgc cccagtcggt gtgctcctca tccttgacga
      241 cgtacgcgac gtagtcgacg tcgaacgcgc gccgcatcag ctccaggtcg cggacgacca
      301 cgcaaccgcc cgactggggc gtgtacaacc acttgtgcgg gtcgaacgcg atcgagtccg
      361 cgcgctcgat ccccgagaag aggggacgca ggtcgtccgc gagcatcgcg gggccgccgt
      421 acgcggcgtc gacgtggaac cagagatcct cgtcgcggca cacgtcggcg atctcctcga
      481 gcggatcgac gacgcccgtg gagaccgttc cggcgttcgc caccaccgcg atcgggtgga
      541 cgccggccgc gcgatcctcg gcgatcgccg cgcggagctc gtccacgcgg atccggtagc
      601 cgtcgtccac ggcgaccttc ctgacgttgc ccgacccgag gcccagcatg tccgcgccgc
      661 ggatcgacac gatgtgcgtc tcggtcgaga cgtacaggcc gagcgtggga ccggcgccga
      721 cgccctcttt ccggatgtcc cagcctgcct tggcatcgcg cgccaccttc agcgcgatga
      781 agttggccat cgccccgccc gaggtcagcg cgccacccga gccgacgggc agaccgaaga
      841 tctcctgcgc gaaccatcgg gtgaacgcca gctcgatctc ggtccccgat ggtgcgaggc
      901 gccacccccc ggtgttcatg ttcaggcccg aggcgagcag atcggcggcc gcgcccggca
      961 ccgtccccgc gcccgagatg taggcgtaga agcgcggatg tccgaggtac ccggagtagt
     1021 cgaacgtcat cgtccgcagg tactcgagga tctcgtccgc gggcatcggc tcctcgggaa
     1081 cggggatcgc gacggcgtcg cgcacctcct ggacccccca gccccgtgag accgggagat
     1141 cgcggagccg ggtgaggagc tcctcgtaga tgtcggcgac cccgtcggtg aacgcgcgcg
     1201 cgcgcttcgg gtcccagtcg agatcgcgga cgggctcggg ccgctcgacc atcacgcctc
     1261 cttcgtcgcc gcgagcacga agccctcttt cgccatgaag cggcggaagc cctcgtcgcc
     1321 gaagacctcg tagagggctt cgatctgact ggcgtgctcc ttgatcgagg cgatcttctg
     1381 atcgaggttc gcgggcggga gctcgaagta caggtcgatc tcctcgtccg gcacgacgtt
     1441 cggggtgccg ggaaggaaga tgttgaacgg cgcgagcttc tcgagccatt cgtcggccat
     1501 gctcgtcggg aagacagctt ggtacaggcg cgctccggcc ttcgcgacct gagcgaacgc
     1561 ctcgccggac catcgcgaca cgtcctggtg cgctacatgg ccggtcatcc cctcaggccc
     1621 gaacgtgagg atcgtgtcgg gatcgatctc cttcatgagc gtgcgcacct gctcggcgcc
     1681 ggcagggtcg aggcccgtct ccatgtcgat gtccaccgga ccctcgagga agtgatgctc
     1741 cgtcacgccg aggacgtcga ggctcctgag gagctcgttc gtgcggacgt cgcccatcga
     1801 ctccggcggc caccgctcct cgtccatcga cccgccctca ccgcgcgtcg cggtgacgtc
     1861 gacgacgcgc tgtcccgcct ccacggcggc cgccatcaga ccggcggaca ggtagatgtc
     1921 gtcgtccgga tgcgcccaga cgccgaggat cgtcccgagg tcggccacgt cgatcatgcg
     1981 gacggatgct agccgggcaa cgggtgatgc ggctagagcg tcccgctagg ggctcgcggt
     2041 gcggatccgc tcgcggatcc gctcggccaa tccctccggg agcacttcgc cgcgacactt
     2101 cgtcgcgatc aggagcttca tgtgcttctt gaactcagcg tggtcgttgc aatcgctgca
     2161 tccggcgagg tgcgcctcga cctggaccgt gagctcacgg agacactcgc cgtcgacgta
     2221 gcgctcgacc tcgcgaagcg tctcatcgca ccccatgtcg cccttctcac ccatcgacga
     2281 gtcctcgctc cttggctacg ccgtacaacg cgcgctccag ggctttcctt ccccggtgca
     2341 ggcgcgacat caccgttccg atcggcacgt ccgtgatctc cgcgatctcc ttataggaga
     2401 agccttccac atctgccagg agcaccgcca tccggaagtt ctcggggatc gactcgagca
     2461 cctccttcac gtcggcatcg gggatcttct cgagcacgtc ctcctcggcc gagcgctcga
     2521 cgctcttggc gcccaatcgg tcgaataaga accattcgtc gatgtcgtcg ggaccctcga
     2581 cggtcttcgg ctcgcgctgc ttcttccggt agatgttgat gaaggagttc gtgaggatgc
     2641 ggtacagcca tgccttcagg ttcgttccct cccggaacga cgcgaagccc cgataggcgc
     2701 ggagcgtcgt ctcctggagc agatcctcgg cgtcggcggg gttcctcgtc atccgcaacg
     2761 cagctccgta gaggccgggg agcagcggga gcacatcccg atcgaatcga tccaggaggt
     2821 cctggtcggc cggtgccggc accctgctcg cgttcgccat cgagtccagt atccctggtg
     2881 cgagggcctg cgcctgcgtc ggtcgtcaca actacaaggc ggtgggaaga ttgggccatg
     2941 cccgagcaag accgcaaacc gttcggacgc cgccccgttc cgggctcagc acagccacgg
     3001 ccgagatcgg cactggtcgt cgtgctcgtg gtggccggct ccctggtgct gttcaacgcg
     3061 ttcctcacgc aggcggcatc cacccccgtg ccgttctcgg agttccggag ggcgatcgac
     3121 aacggcgacc tcgtgaagga ggagccggtc acgatcacga ccaccacgat cagcggtgtg
     3181 gtccgaaccc ccgacggcga cgagacctac gtggcgacga tcccgcccgg gtacgttccc
     3241 accgatctcg tcgacgacct cagcgcgcgg ggctacgagg tcgatggtca gcagccgaac
     3301 gcgct
//
LOCUS       DASUVE010000345         5539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_168795, whole genome shotgun sequence.
ACCESSION   DASUVE010000345 DASUVE010000000
VERSION     DASUVE010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168795"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1390)
                     /locus_tag="VF036_07455"
     CDS             complement(197..1390)
                     /locus_tag="VF036_07455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX6265101.1"
                     /translation="MPTTVLLCDTPDEVARFQYHLLREHDLEVEVTTDAFRGVEAAVR
                     SRPDVIVSELTLEGLGGAELVKRFLASSPASRFVAHTVVRDPVRVTEVLAAGASGYVL
                     KEDPVEDSLRAIRAASTGAVTLSGQVAGLLSTELARAVSRSHELETELVALRHDVAQG
                     STAKADFLANISHELRTPVTVAKGIGYVLKNPAVPDEERNEFLGQLTASLDKLMGIVD
                     ELITLSELERGTFELSLVETDLAPLVRHAIDEVRMRYPGIPIAAKVSDGLLCIADGPR
                     LGSVVRELLDNACRYSPFGRAVELVARDLAEGVTVMVTDRGEGLDRAVAARAFEQPFS
                     TGEGVLHKEKSGVGTGLHLARQLVVEHGGVMWVDPLPGGGTRASFCIPHRRGERIEAP
                     PAHVA"
     gene            complement(1437..2078)
                     /locus_tag="VF036_07460"
     CDS             complement(1437..2078)
                     /locus_tag="VF036_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6265102.1"
                     /translation="MTGIRALIVDDHPVTREGLRTALSMSDDAVVVVGEAASGQEAID
                     QARDLSPDVVFMDVRMPGMDGIEATRRIREASPETRVILITIDESRGAISEAIQAGVS
                     GYLLKDASPDALVDAARNAVEGNAVIHPQLTKTFIEEVHLAETEGRSTPLSKREREIL
                     QKVADGATTRQVASDLGISPHTVKTHLERIFEKLGANDRAQAVAIAIRTGIVH"
     gene            2279..2599
                     /locus_tag="VF036_07465"
     CDS             2279..2599
                     /locus_tag="VF036_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017870951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX6265103.1"
                     /translation="MSTNGGAPSEEQVLEALKSVIDPELGINIVDLGLVYEVDVAEGG
                     DVDIQYTLTTMGCPIGPLIEDQMRAFLAPLDGIGEVRPEMVLRPAWNPEMMSEEAKAA
                     LGMF"
     gene            2887..3729
                     /locus_tag="VF036_07470"
     CDS             2887..3729
                     /locus_tag="VF036_07470"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017613065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX6265104.1"
                     /translation="MTDYAKPDVLVSTDWLEEHLDDPAVRPIEVDEDTAAYEKGHVRG
                     AVAWNWNTDLHTDVGRDYRDQAGFSELLSAAGVAPDTTVVLYGGNNNWFAAYAYWLLK
                     LRGFDNVKLLDGGRKKWELESRELTQDAPSYQATGFSISGAERPEIRALRDEVLQKAG
                     STKFVDVRSPEEFRGEKIAPDHLPQEQAQVPGHIAGAANIPWAKAANEDGTFKSADEL
                     RELYAAEGITGEGEVIAYCRIGERSSHTWFALQELLGFENVKNYDGSWTEYGSLVGAP
                     VELG"
     gene            3810..4106
                     /locus_tag="VF036_07475"
     CDS             3810..4106
                     /locus_tag="VF036_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265105.1"
                     /translation="MGCLFVILGAFTPRLLFLIVWIARPAYVDAVFDTFIFPLLGLIF
                     LPFTTLLWVLLDAPPGGVVGFDWFWIVLAVLMDLSHYAGAYTQRGRYSGSAATA"
     gene            complement(4153..4587)
                     /locus_tag="VF036_07480"
     CDS             complement(4153..4587)
                     /locus_tag="VF036_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX6265106.1"
                     /translation="MQGHPAGSVSRLLAAIIDLAITVGAMLLFYLGIGAVAFVFRPRA
                     FRWPDPGAITLGGAWWLLLVLYLTFSWTASGRTIGAQVMGLRVTDRSGERLHGPRAFL
                     RAVICAAFPVGLVWCAIDRRRRALHDLLVGSQVTYDWIPNTA"
     gene            complement(4614..>5539)
                     /locus_tag="VF036_07485"
     CDS             complement(4614..>5539)
                     /locus_tag="VF036_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265107.1"
                     /translation="VSVVVIDPLPSADEDDRTAAEVAADRSRGILEGGLLVTVGLTAL
                     AVDGIVSAILRVGGDTISPGGGTDGEGPVEGPSTEPPPTDTAAVVVGAALNVAFEASR
                     GLMRIAANLERAIRPLTVVAMIPPVDRAARRLEEMATRRNETWHAERNESMRAADLFA
                     DAVVPQLVDAVVDRLDLTDLVLERVDLRAVISSIDIDEIVGRVDVTAIVDGLDIDAVA
                     ARVDVQKIVDRLDLVAIAEGVVAELDLPAIIRGSTETMAAETVDGVRVQSMHADRFVA
                     RVVDRALLRRDGGANGSPDPSGPTAPAEDES"
BASE COUNT          891 a         1810 c         1967 g          871 t
ORIGIN      
        1 gccgtcacga ggttgaacgg ggtcggtgac ggcgtgacga ggagttcctg gagcggtgtg
       61 gtcgtggggg acgcgggggt gcatgcggac atcgcgacga cgaccccgat cccgaggagg
      121 cttctgcgca cccgcacctg atcctcgagc caggatactt cccagcacct ccgtaggtcc
      181 aacggggggc ggtgattcag gcgacgtgcg ccggaggggc ctcgatccgt tcgccccggc
      241 gatgggggat gcagaaggag gctcgggtac cgccgccggg caagggatcg acccacatca
      301 cgcccccgtg ctcgaccacg agctgccggg cgagatgcag cccggtcccg acccctgact
      361 tctccttgtg gagcacgccc tcccccgtgg agaagggctg ctcgaaggct cgagccgcga
      421 ccgctcggtc caggccctcg cctcggtcgg tcaccatcac ggtcacgccc tctgcgaggt
      481 cccgcgcgac gagctcgact gcacgaccga acggggagta gcggcacgcg ttgtcgagga
      541 gctcccggac caccgacccg aggcgaggcc cgtccgcgat gcagagcagg ccgtcggaca
      601 ccttcgccgc gatcgggatc cccgggtagc gcatccggac ctcgtcgatc gcgtggcgga
      661 cgagcggggc gagatccgtc tcgaccaggc tcagctcgaa cgttccgcgc tcgagttcgg
      721 acagggtgat cagctcgtcc acgatcccca tcagcttgtc gagcgacgcc gtgagctgac
      781 cgaggaactc gttgcgctcc tcgtcgggga cggccgggtt cttcaagacg tagccaatgc
      841 ccttcgctac ggtgaccggc gtccggagct cgtgggagat gttcgcgagg aagtcggcct
      901 tcgccgtgga cccctgggcg acgtcgtggc ggagggcgac gagctccgtc tccagctcgt
      961 gagaacggct gaccgcgcgc gcgagctcgg tcgagagcag cccggccacc tgcccggaca
     1021 gcgtgaccgc gccggtggag gccgcgcgga tggctctgag ggagtcctcg acggggtctt
     1081 ccttcagcac gtagcccgat gcaccggcgg cgaggacctc ggtcacgcgg accgggtcac
     1141 ggacaacggt gtgggccacg aaccgggagg cgggggagga ggcgaggaat cgcttcacca
     1201 gctcggcgcc gcccagcccc tcgagcgtga gctcggacac gatcacgtcc ggccgcgacc
     1261 ggaccgcggc ttccacgccc cggaaggcat cggtcgtgac ctcgacctcc aggtcgtgct
     1321 cccgcagcag gtggtactgg aagcgagcga cctcgtcggg cgtgtcgcag agcagcacgg
     1381 tggtgggcat ctgctgttgg tatcggcgcg tgggaggacc ggttgaggtc ggccgttcag
     1441 tggacgatgc cggtgcggat ggcgatcgcc acagcctgtg cccggtcgtt cgccccgagc
     1501 ttctcgaaga tccgttcgag gtgggtcttc accgtgtggg gcgagatccc caggtcgctc
     1561 gccacctgcc tcgtcgtggc cccgtcagca accttctgga ggatctcccg ctcgcgcttg
     1621 gagagcgggg tggacctccc ctcggtctcg gcgaggtgga cctcctcgat gaaggtcttc
     1681 gtgagctgcg gatggatcac ggcgttgcct tcgaccgcgt tgcgagcggc atcgacgagc
     1741 gcatcgggtg acgcgtcctt gagcagatac cccgacaccc cggcctggat cgcttcggag
     1801 atggctccac gcgactcgtc gatcgtgatg aggatgaccc tcgtctccgg agatgcctcg
     1861 cggatccgtc gagtggcctc gatcccgtcc atcccgggca tgcggacgtc catgaagacc
     1921 acatcgggag acaggtcacg ggcctgatcg atcgcctcct ggccgctcgc cgcctcaccg
     1981 acgaccacca cggcgtcgtc cgacatcgac agggcggtcc gaaggccctc ccgggtcacg
     2041 ggatggtcgt cgacgatcaa cgctcggatc ccagtcatcg gtcgggtgcg gccctccttt
     2101 cgggtctccg gcggtctccg gggcgcgtcc tgtcgcgtgc cttggcgatc ggagcggtag
     2161 tctggtcctt aggatagttc gtatctggac cgaggtatgt atgcctgagc acgaaagcgc
     2221 tatatcgcaa gcacacaaca cccctgaggg atcggccgag ccgacggtcg ccaccgccgt
     2281 gagcaccaac gggggagcgc cgtcggaaga acaggtgctc gaggcgctca agagcgtgat
     2341 cgaccccgag ctgggcatca acatcgtcga cctgggcctc gtgtacgagg tcgacgtggc
     2401 cgagggcggc gacgtcgata tccagtacac gctcacgacg atggggtgtc cgatcgggcc
     2461 gctgatcgag gatcagatgc gcgccttcct cgcaccgctc gacgggatcg gcgaggttcg
     2521 tcctgagatg gtgctcaggc cggcctggaa tcccgagatg atgtcggagg aggcgaaggc
     2581 cgccctcggg atgttctgac acctcgaacc ggcgttgacg cctgctgcgg cgtgcgatag
     2641 tcgtggcagc tgcagtttcc gatccgagag gtgggatccg aaggtggtcc ggacccgggc
     2701 actgggcatc gtaggagcga tgatcccgtc gaagctgtgc tgttcgaagg ccgacggagg
     2761 atcgctgcgc gcgcggtcga cgtagccgag gaagacatct tggtgatgcg agcggccgcg
     2821 aggtgcggcc gctcatgcgt ttccggtcct atcgcggggc cgtggagaga agagggggga
     2881 caggcgatga cggactacgc gaagcccgac gtgctcgtga gcaccgattg gctggaggag
     2941 catctcgacg atcccgccgt gcggccgatc gaggtggacg aggacaccgc cgcctacgag
     3001 aagggccacg tccggggcgc ggtcgcatgg aactggaaca ctgacctgca cacggacgtc
     3061 ggtcgtgact atcgggacca ggccgggttc tccgagctgt tgtcggcggc cggcgtcgcc
     3121 cccgacacga cggtcgtcct ctacgggggg aacaacaact ggttcgcggc ttacgcgtac
     3181 tggctcctga agctccgtgg gttcgacaac gtgaagctgc tcgacggcgg tcgcaagaag
     3241 tgggagctgg agagtcgtga gctcacccag gacgcgccgt cctaccaggc cacggggttc
     3301 tcgatctccg gcgccgagcg accggagatc cgggcgctcc gcgacgaggt gctgcagaag
     3361 gccggctcca cgaagttcgt ggacgtacgc tcgcccgagg agttccgcgg cgagaagatc
     3421 gcgccggacc atctgcccca ggagcaggcg caggtgcccg gccatatcgc gggcgcggcg
     3481 aacatcccct gggcgaaagc ggcgaacgag gacgggacgt tcaagtcggc ggacgagctg
     3541 cgcgagctct acgcggccga ggggatcacg ggcgagggcg aggtgatcgc gtactgccgc
     3601 atcggggagc gctcgtccca cacgtggttc gcgctgcagg agctgctcgg gttcgagaac
     3661 gtcaagaact acgacggctc gtggaccgag tacggctcgc tcgtgggggc gcccgtcgaa
     3721 ctggggtagc cgtggaggga gcgagccggc cgcgacgcga cagcgggacg gggatatgct
     3781 ccgcgggtca tcgagagagg ggaagaggca tgggttgctt gttcgtgatc ttgggggcgt
     3841 tcacaccgcg actcttgttc ctgatcgtct ggatcgcccg gcccgcgtat gtcgacgcgg
     3901 tgttcgacac gttcatcttc ccgttgctcg ggctcatctt ccttccgttc acaaccttgc
     3961 tgtgggtgct cctcgatgca ccaccgggcg gggtcgtggg attcgattgg ttctggatcg
     4021 tcctggcagt gctgatggac ctcagccact acgccggggc gtacacccag cgggggcgtt
     4081 actcgggcag cgcggctacc gcctgacctc gcccggatcc atctctcgcc tgaccccgcg
     4141 cttcgcgcgg ggtcaggcgg tgttcgggat ccagtcgtag gtgacctgtg agccgaccag
     4201 gaggtcgtgg agggcccgcc tgcggcggtc gatggcacac cacaccaacc cgacagggaa
     4261 cgcggcgcag atgaccgctc gcaggaacgc tcgggggccg tgcagcctct cgccgctccg
     4321 atcggtcacc cggagaccca tcacctgcgc gccgatcgtt cggccgctgg cggtccagga
     4381 gaacgtgagg tacaagacca ggaggagcca ccaggctccg ccgagggtga tcgcgcctgg
     4441 gtccggccat cggaacgcgc gaggccggaa gacgaacgcg accgctccga taccaaggta
     4501 gaagagcagc atcgctccga cggtgatggc gagatcgatg atcgcggcca gcagccgaga
     4561 aacggaaccg gcgggatgtc cctgcacctc ccgggcatcc ggcgtcggca ggatcaggac
     4621 tcgtcctccg caggggcggt cgggccggac gggtcgggtg acccgttggc ccccccgtcg
     4681 cgacggagga gcgcccgatc gacgacgcgg gcaacgaagc ggtccgcgtg catgctctgg
     4741 acccgaacgc catcgaccgt ttccgctgcc atcgtctcgg tcgatccccg gatgatcgcg
     4801 gggagatcga gctcggcgac cacgccttcg gcgatcgcga ccagatcgag gcgatccacg
     4861 atcttctgga cgtcgacgcg ggcggcgacc gcatcgatgt ccagcccgtc gacgatggct
     4921 gtcacgtcca cacggcccac gatctcgtcg atgtcgatgc tcgagatcac ggcgcgaagg
     4981 tcgacccgtt cgaggacgag gtcggtcaga tcgagccgat caaccaccgc atcgaccagc
     5041 tgaggaacca ccgcgtccgc gaacaggtcc gcggcacgca tgctctcgtt ccgttcggcg
     5101 tgccaggtct cgttcctgcg ggtggccatc tcctccagac gccgagccgc tcggtcgacc
     5161 ggggggatca tggccacgac cgtgagcggc cggatcgctc gttcgagatt cgcagcgatc
     5221 cgcatgaggc ccctgctcgc ctcgaacgcg acgttcagag cggcgccgac gaccaccgcg
     5281 gcggtgtccg tggggggcgg ttccgttgaa ggtccctcga ccggtccctc cccgtccgtc
     5341 ccaccgccgg gcgagatcgt gtcgccgccg actcgcagga tcgccgacac gatcccgtcc
     5401 accgcgagcg ccgtgagacc gacggtaacg agcaggccac cttcgaggat gccgcgtgag
     5461 cggtcggcgg caacctccgc agcggttcga tcgtcctcgt cggcggaggg gagggggtcg
     5521 atcacgacca ccgagacct
//
LOCUS       DASUVE010000346         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1488181, whole genome shotgun sequence.
ACCESSION   DASUVE010000346 DASUVE010000000
VERSION     DASUVE010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488181"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VF036_07490"
     CDS             <1..432
                     /locus_tag="VF036_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265108.1"
                     /translation="ERDRGDRRVVPGAAPFGSLDFLYVPSRDVAEDLRWFGEVLGGRV
                     VFAIDSMGTRVAAVELSDDPPLVLLTDHLEGDRPVLVYRVPDLNEALDTLEGRGWRRE
                     HRFEIPHGPICSFEGPDGHRVALYQLTRPEVAAHFEGRRDF"
     gene            complement(429..869)
                     /locus_tag="VF036_07495"
     CDS             complement(429..869)
                     /locus_tag="VF036_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014689916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX6265109.1"
                     /translation="MRHAGRVADWNKGIIEEFRANGGKVGGYFDGATVLLLHTIGRRS
                     GKDHVTPLVYLPDGERWAVVGSKGGAPADPDWVRNLEANGEATIEVGSETIPVHATQI
                     LRGGPEWDDLYGRQVARREGFADYLVKTEGIRTIPVIVLERRAP"
     gene            complement(881..1198)
                     /locus_tag="VF036_07500"
     CDS             complement(881..1198)
                     /locus_tag="VF036_07500"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX6265110.1"
                     /translation="MTIERGEVWWAETPESSKGRPFLVLTRNEAIPVLRTLLVAPISR
                     TIRRIPTEVSLGLSEGLPVASAATFDAVLTVPKSMLVRRMGALAPERHAELCEAFAAA
                     VDC"
     gene            complement(1200..1451)
                     /locus_tag="VF036_07505"
     CDS             complement(1200..1451)
                     /locus_tag="VF036_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX6265111.1"
                     /translation="MSQQIAIRIPDDLAAQLEDLVSSGRFETKADAVRTALESMIDAA
                     RRADIGRQIVEGYRRVPQEDGDVAAASQAADRSIDEEPW"
     gene            1531..2715
                     /locus_tag="VF036_07510"
     CDS             1531..2715
                     /locus_tag="VF036_07510"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX6265112.1"
                     /translation="MASERIVELLRSAPLIDGHNDLVWALRKAAEKGESHDVGSSAPS
                     LHTDLPRLERGGVRGQFWSVYVPSDLADPHAAVTMTLEQIDALFALVRSHPGRLELAR
                     SADDVERIAGAGRVASMIGVEGGQSIGSSLGALRILAGLGAGYLTLTHNDDTPWADSG
                     TGERAHGGLTTFGEEVVRELNRLGILVDLSHTSEDTMRQAIEVSAAPVLFSHSNARAL
                     CDVPRNVPDDVIELVGRTDGVICATFVPWFLTAEGAEANEAEWREIRRLKEEHPDDPD
                     AVRAAVEEMEETQSTPASSVSDVADHIDHIRDVAGIDHVGVGSDFDGLSGMPDGLQDV
                     STYPALIEELAERGYDDEALAKAAGRNVLRVMRAAEWVAAGYGWAGFKGAREGTSGRD
                     GP"
BASE COUNT          379 a          950 c          968 g          429 t
ORIGIN      
        1 gaacgcgatc gcggcgatcg ccgcgtagtg cccggtgcgg cgccgttcgg ctcgctcgac
       61 ttcctctacg tccccagccg cgacgtcgcc gaggatctcc ggtggttcgg cgaggtcctc
      121 ggtggccgag tggtgttcgc gatcgactcg atggggacgc gggtggcggc cgtcgagctg
      181 agcgacgatc ctccgctcgt cctgttgacc gatcacctgg agggcgatcg gccggtgctc
      241 gtgtatcgcg tccccgatct gaacgaggcg ctcgacaccc tcgagggtcg gggctggcgt
      301 cgggagcacc ggttcgagat cccgcacggc ccgatctgct ccttcgaggg cccggacggg
      361 catcgagtcg ccctgtatca gctcacgcgg cccgaggtcg cggcgcactt cgagggccgt
      421 cgggacttct agggcgcgcg gcgttccagg acgatcacgg ggatcgtccg gatgccttcg
      481 gtcttcacga ggtagtccgc gaagccttct cggcgtgcga cctgccgccc gtagaggtcg
      541 tcccactcgg ggccaccgcg gaggatctgc gtggcatgca ccgggatcgt ctcggacccc
      601 acctcgatcg tcgcctcgcc gttcgcctcg aggttgcgca cccaatcggg gtccgcgggc
      661 gcgccgccct tggagccgac caccgcccag cgctcgccgt cggggaggta caccaggggc
      721 gtgacgtgat ccttcccgct acgacgaccg atcgtgtgga gcaggaggac cgtggcgccg
      781 tcgaagtagc cgccgacctt ccccccgttg gcgcggaact cctcgatgat gcccttgttc
      841 cagtcggcca cacgcccagc atggcgcatc gccgatcccc tcagcaatcg acggccgcgg
      901 cgaacgcctc gcacagctcg gcgtggcgct ccggcgcgag ggctcccatc ctgcggacga
      961 gcatcgactt cgggaccgtg agcacggcgt cgaacgtcgc cgcggaggcg accggcaggc
     1021 cctcggacag tccgagcgag acctccgtcg ggatgcgccg gatcgtgcgg gagatcggcg
     1081 cgacgagcag ggtgcgaagg acggggatcg cctcgttccg ggtgaggacc aggaacggtc
     1141 gacccttcga cgactcgggc gtctcggccc accagacctc gcctcgctcg atcgtcatct
     1201 caccacggct cttcgtcgat cgagcggtcg gcggcctggc tcgccgcggc gacgtcgccg
     1261 tcctcctggg gaacgcgccg gtatccctcg acgatctgcc ggccgatgtc cgccctcctc
     1321 gccgcgtcga tcatcgactc caacgcggtg cggacggcgt ctgctttcgt ctcgaagcgg
     1381 cccgaggaaa cgagatcctc gagttgggct gccagatcgt ctgggatgcg tatcgctatc
     1441 tgttgtgaca tgcggaaagc ataccactcc cttggtccgg tcgcgggagc ggccgcagcg
     1501 gttcccgcaa gctgggcgta ccatccgcgg atggcgtcgg agcggatcgt cgagctgctc
     1561 cgatccgcgc cgctgatcga cgggcataac gacctcgtgt gggcgcttcg gaaggcggcg
     1621 gagaagggtg agtcgcacga cgtcggctcg tccgctccga gcctccacac ggatctcccg
     1681 cgcctcgagc ggggaggagt ccgcggacag ttctggtccg tctacgtccc ctccgacctg
     1741 gccgaccccc acgcggccgt gacgatgacg ctcgagcaga tcgacgcgtt gttcgcactc
     1801 gtgcggtcgc atcccggccg cctcgagctc gcccgctcgg ccgacgacgt ggagcggatc
     1861 gccggggccg ggcgcgtcgc gtcgatgatc ggggtcgagg gcgggcagtc gatcggttcg
     1921 tccctcggcg ccctgcggat cctcgctggc ctcggtgcgg gctacctcac cctcacccac
     1981 aacgacgaca cgccgtgggc ggactcgggg accggggagc gggcacacgg cgggctcacg
     2041 accttcggcg aggaggtcgt ccgcgagctg aaccgcctcg ggatactcgt cgacctctcc
     2101 cacacgtccg aggacacgat gcggcaggcg atcgaggtct cggctgcccc ggtcctcttc
     2161 tcgcactcga acgcccgcgc tctctgcgac gtgccgcgca acgtgccgga cgacgtgatc
     2221 gagttggtgg gtcgtacgga cggcgtcatc tgtgcgacct tcgtgccctg gttcctcacc
     2281 gctgagggcg cggaggccaa cgaggccgag tggcgtgaga tccgcaggct gaaggaggag
     2341 catccggacg accccgacgc ggtccgcgcc gccgtcgagg agatggagga gacccagtcg
     2401 acgccggcca gctcggtctc cgacgtggcc gaccacatcg accacatccg cgacgtggcc
     2461 ggcatcgatc acgtgggcgt cggaagcgac ttcgacgggc tctcgggcat gcccgacggg
     2521 ctccaggacg tgtccacgta tccggccctg atcgaggagc tcgccgagcg tggctacgac
     2581 gacgaagccc tcgcgaaggc ggcggggcgc aacgtgctgc gggtcatgcg ggcggccgag
     2641 tgggtcgcgg ccgggtacgg ttgggccggt ttcaagggcg cccgtgaggg gacctctggc
     2701 cgggatggac cctagggccc ccgggg
//
LOCUS       DASUVE010000347         8981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1488941, whole genome shotgun sequence.
ACCESSION   DASUVE010000347 DASUVE010000000
VERSION     DASUVE010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488941"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..822
                     /gene="upp"
                     /locus_tag="VF036_07515"
     CDS             199..822
                     /gene="upp"
                     /locus_tag="VF036_07515"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX6265113.1"
                     /translation="MSLTVLDHPLAHDLLSRLRERGTGPAEFRTLTRRLGWLLVIEAT
                     RDLLTEKVTVDTPIEETKAARLAEPLVAIPVLRAGLGLLDAVTDLYPDTIVGYLGLER
                     DEETLEPRDYYAKLPRMEGHRALVLDPMLATGGSGSAAIAHIRAHANPTSISFVCIVA
                     APEGIARLEADHPEVPIVAGALDRRLNDVGFIVPGLGDFGDRLHGTD"
     gene            complement(825..1805)
                     /locus_tag="VF036_07520"
     CDS             complement(825..1805)
                     /locus_tag="VF036_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6265114.1"
                     /translation="MDLTDIGASIRIAFIYFTILYLTGLGGLFSERSGIVNIGLEGLM
                     VVGTVTGAAGVAMFAGPAPEGMGLGMGGVVLGLLFGALCGALFASVHAVATINFRVDH
                     IVSGVVINLVAIGLARFLSFIFFEQATQSDPGGPHLPRIDIPLLSDIPGGLGVAFQNL
                     SPMFLFAAFLVFPVTFALYRTRWGLRLRSAGENPEATRSLGVAVAPLRWQGVLLSGAL
                     AGLSGAYLSVEVVGNWREGQTLGLGFIALAALILSNWDPRRLMVAAFLFGFAQAIPLR
                     LDDAPIISALPPQFINMIPYVVTIVAIAGFVGKVRPPAAAGRAYEGAGGT"
     gene            complement(1805..2983)
                     /locus_tag="VF036_07525"
     CDS             complement(1805..2983)
                     /locus_tag="VF036_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6265115.1"
                     /translation="MSAASPAVPVPRSRPPWLDTALGQGLALLSAFAIAIAVGSLIII
                     LYGESPVTVYGAMLRFAFKDPTSIGAVLAIATPLIFAALAVSVCFKAGLFNIGVEGQF
                     LVGMVTASWAALTFDFMPGVSHLIMVLLFAVIGGMVYAAVPGILKVTTGAHEVVTTIM
                     MNGIAISIVAYALNGPLKFTDAPSGQNVDLRTDIFAPSALVPDLGHFFGVSGSAHLSW
                     LFPIAIVASVLVWFLFRRMRLGFEARAVGSSAGSARAGGIAIGAVQIKLFLISGALAG
                     LTGMQQVLVERGALVQNYEAQLGFTGIAVAFLGQNNPIGIVFAAAIWAILSRGETALQ
                     IETDVPREFVIILQGILIFCVVVVYQIAKRRLARRELQRAGAEVGLRDTEPDEGSDDA
                     "
     gene            complement(2983..4518)
                     /locus_tag="VF036_07530"
     CDS             complement(2983..4518)
                     /locus_tag="VF036_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018975443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6265116.1"
                     /translation="MSDQREAGRAGAPPFVVMEHITKRFPGVVANDDVSLEVRAGEVH
                     ALIGENGAGKSTLMRVLYGMYPADEGRIVVNGEDVRITSPRVAIEHGIGMVHQHFVLV
                     DPFTVTENVILGDEGEAVLDMDAAHEKVAALAKTYGFHVLPSAIVEDLSVGEEQRVEI
                     LKALYRGVDLLILDEPTAVLTPLEAKDLFENLRQLRDDGKAIVFISHKLDEVLDIADR
                     ITVLRRGQIVGETRPAETSKAKLAEMMVGRPVLFRLDKPEVEIGAPVLEVEGLLGEGK
                     LNGISLQVREREILGIAGVEGNGQRELAEALIGLRQPAAGTIRLAGQDIAGWSVADIR
                     NAGVAYIPEDRQEQGLVLDMTLWENSLLGRGDDRPFSGRTGFLFIQKIKDMARRLVKA
                     FDVRARSINVNASTLSGGNQQKLVLARELDTSPKLLVASQPTRGLDVGAIEFVWRQIL
                     RQKEEGRGVLLISAELDEIYALSDRIVTIYEGRITGEYSPDAPPEDLGIGMLGATETA
                     EAS"
     gene            complement(4540..5628)
                     /locus_tag="VF036_07535"
     CDS             complement(4540..5628)
                     /locus_tag="VF036_07535"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6265117.1"
                     /translation="MPKWLRMLAVLAVLALVGAACGDDTESPPGGDSEAPECNADLSV
                     GVAYDIGGIGDKSFNDAANAGLTAAIDEGLVCEENTESIEPDATGSNRDENVLALADA
                     GHNLVIAVGFAFSPGINENAGDYTDTQFAIIDGYATCGTACGLTNDGLTNVTDLTFKE
                     HEGSYLVGAAAATQADKDGCTTVGFLGGQTGPLIEKFQAGFEAGVADVNDGIEVLVQY
                     IGDDVTAFNDAVKGEALSTGMYDDGACIIYHAAGASGAGLFNAAVAADALAIGVDSDQ
                     YQLVSAEQQPHIMTSMLKRVDTAVRDAIEQSGDGSLEAGGQVFGMAEGGVDYSKSNTE
                     LMTDDIITLHDDLKQQIIDGDITVPDTP"
     gene            5761..6183
                     /locus_tag="VF036_07540"
     CDS             5761..6183
                     /locus_tag="VF036_07540"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6265118.1"
                     /translation="MSEDEDLFRELHARLAASPFHTWAGMEVVSAARGAVTVAMELQE
                     RHANLQGFVHGGMLSILADTACGLSIRSAMEPGRLHVTADLDIHFLAPAKPGRVLGRG
                     IAVKVGRSLAFAEASIEDGGGRLLAKAQSRFSVGGTET"
     gene            complement(6180..6563)
                     /locus_tag="VF036_07545"
     CDS             complement(6180..6563)
                     /locus_tag="VF036_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX6265119.1"
                     /translation="MSVKFLSDEWAQALKAELNESEEFRRAAAGQHATIQQVITGGGA
                     DTHYWITIEDGRIDLGVGDVEGEDATITQSYDSAAGLARGELSPVTAFMTGKLKIAGN
                     MGLILGLQGALAQLPAAMAKIDVEY"
     gene            complement(6631..7278)
                     /locus_tag="VF036_07550"
     CDS             complement(6631..7278)
                     /locus_tag="VF036_07550"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6265120.1"
                     /translation="MTERVLYLIRHGQSDFDWQGERWASARGEQWDPPLSERGAEQAA
                     LLAGRLLVMDLDPFVVYSSPLRRANETAEAFARLAGTRVTVDDELVEAHIGGWEGKPF
                     EEIMGSDPDVVQHIRNQRAIWHRAPGAEGGDGFRTRVRTAVDAIVDHHPSENVLVFAH
                     GGVINAYVGDLLGLRQEMFFLPENTSLNSVDVDGETRTVRFLNDVLHLTDPLFFE"
     gene            complement(7275..7670)
                     /locus_tag="VF036_07555"
     CDS             complement(7275..7670)
                     /locus_tag="VF036_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265121.1"
                     /translation="MAVGEHARARMWETGGRWFAHILDVSAYIEVTATSRAGCLEELR
                     KVTGDDVDLMIEVIPAVVGVAEAAEIMGWDKRRVITYIDRGSFPEPLTTLASGRIWIR
                     EDIEGYADHWRATHPPAPPRRSTTLSAPA"
     gene            complement(7888..8175)
                     /locus_tag="VF036_07560"
     CDS             complement(7888..8175)
                     /locus_tag="VF036_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265122.1"
                     /translation="MPRKNRRDPEYFQPQEAPRPRSAAPRWAELPGYDVRHVGGEKEY
                     RCPGCDHVVRAGIWHLVVIPLDAPDERRHWHTECWRKELRRIGAYRAPPPE"
     gene            complement(8175..8843)
                     /locus_tag="VF036_07565"
     CDS             complement(8175..8843)
                     /locus_tag="VF036_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX6265123.1"
                     /translation="MAATEESLRLETVKGPIAAVYAHPRSPFATIVVAHGAGAGMDHP
                     FLVGFARACIEEGIAALRFNFPYVERGRRSPDPESVLRDAWRAAFEAAVDRATGGPVW
                     ASGKSLGGRIASMAAADADIDPAGLVFLGYPLHPPGKPERIRDEHLYRIEAPMLFLQG
                     TSDPFASPELLRSVVEKLGALATLVPFEGGGHSFEVRGRKRDPREVGASLAPHAAAFI
                     RDHG"
BASE COUNT         1453 a         3129 c         2995 g         1404 t
ORIGIN      
        1 ccccggagac gcaacgaccc cgggtcttcg gcccggggtc cctgtcgctt cggctgcagg
       61 tgactactcc gggcagcgcc cgaaggagaa gtaccagtcg cagaagcacc gctgctgcat
      121 caggcgctca tggtgaccgg caagcgcctc cggtgtcaac cggcgcgcgt gcccccaccc
      181 aggactagga tgcagccgat gtcgctcacg gtcctggacc acccgctcgc gcacgacctc
      241 ctgtcacgac tgcgcgaacg cgggacggga cccgccgagt tccggacgct gacgcggcgg
      301 ctggggtggc tcctcgtgat cgaagcgacc cgcgatctcc tgaccgagaa ggtgacggtc
      361 gacaccccga tcgaggagac gaaggcggcg cgcctggcgg agccgctcgt cgcgatcccc
      421 gtgctccgag ccggcctggg gctcctcgac gcggtcacgg acctgtatcc ggacacgatc
      481 gtcggctacc tcggattgga gcgcgacgag gagaccctcg aaccgcgcga ctactacgcc
      541 aagctccccc ggatggaggg ccatcgagcc ctggtgctcg acccgatgct cgccaccggc
      601 ggatccggca gcgccgcgat cgcgcacatc agggcacacg cgaaccccac ctcgatctcg
      661 ttcgtctgca tcgtggcggc ccctgaaggg atcgcgcggc tcgaggccga tcaccccgag
      721 gtaccgatcg tcgcgggggc gctcgatcgg cggctgaacg acgtcgggtt catcgtgccc
      781 ggtctcggcg acttcggcga ccggctccac ggcacggact gacctcaggt cccgcccgct
      841 ccctcgtacg cgcgccccgc ggcggccggc gggcgcacct tcccgacgaa gcctgcgatc
      901 gccacgatcg tgaccacgta ggggatcatg ttgatgaact gcggcgggag cgccgagatg
      961 atgggcgcgt cgtccagccg gagggggatc gcctgcgcga acccgaacag gaacgcagcg
     1021 accatcagtc gtcgggggtc ccagttcgac aggatcagtg cagcgagcgc gatgaagccg
     1081 agaccgagcg tctggccctc ccgccagttg ccgacgacct cgacggacag gtacgccccc
     1141 gagagcccgg cgagcgctcc tgagaggagg accccttgcc accggagcgg agccacggcg
     1201 acccccaggg accgggtcgc ctcgggattc tctcccgcgg atcggagccg caacccccag
     1261 cgcgtccggt agagggcgaa cgtgaccggg aagacgagga acgcggcgaa caggaacatg
     1321 ggcgagaggt tctggaacgc gacccccagt ccacccggga tgtctgacag cagcgggatg
     1381 tcgatcctgg gcaggtgcgg cccgccgggg tcggactgtg tggcctgctc gaagaagatg
     1441 aaggagagga accgcgccag cccgatcgcg acgaggttga tcacgacgcc ggacacgatg
     1501 tggtcgacac ggaagttgat cgtggccacg gcgtggacgg atgcgaacag ggcaccgcag
     1561 agcgcaccga acagcaggcc caggaccacg ccgcccatcc cgagtcccat cccctcgggg
     1621 gcaggaccgg cgaacatcgc gacgcccgcg gcaccggtga cggtgcccac caccatcagc
     1681 ccctcgaggc cgatgttcac gatgcccgac cgttcgctga agagtccccc gagacctgtc
     1741 aggtagagga tcgtgaagta gatgaacgcg atccggatcg acgcgccgat gtcggtgaga
     1801 tccatcaggc gtcgtccgat ccctcgtcag gctcggtgtc ccggagcccg acctcggcgc
     1861 cggcccgctg cagctcccgc cgagccaggc gacgcttggc gatctggtac acgaccacca
     1921 cacagaagat caggatcccc tgcaagatga tgacgaactc gcgaggcacg tccgtctcga
     1981 tctgcaaggc ggtctcgcct cgcgacagga tcgcccagat ggcggccgcg aacacgatcc
     2041 cgatcgggtt gttctgtccg aggaacgcca cggcgatccc ggtgaacccc agctgcgcct
     2101 cgtagttctg cacgagcgct cctcgctcga cgaggacctg ctgcatcccg gtcagccccg
     2161 ccagggctcc cgagatcagg aacagcttga tctgcacggc cccgatcgcg atgccgcctg
     2221 cccgagcaga ccccgcggac gagcccaccg cgcgagcttc gaatcccagc cgcatccgac
     2281 ggaacaggaa ccagacgagg acgctcgcca cgatggcgat cgggaacagc cacgagagat
     2341 gtgcggagcc gctcaccccg aagaagtggc cgagatccgg gacgagggcg ctcggcgcga
     2401 agatgtccgt ccgcaggtcg acgttctggc cggagggcgc gtccgtgaac ttgagcggcc
     2461 cgttgagcgc gtaggccacg atgctgatcg cgatgccatt catcatgatc gtggtgacga
     2521 cctcgtgggc gcccgtcgtc accttcagga tccccgggac cgcggcgtac accatgccgc
     2581 cgatgacggc gaagagcagg accatgatca ggtgggacac gcccggcatg aagtcgaacg
     2641 tgagcgccgc ccaggacgcc gtgaccatgc ccacgaggaa ctgcccctcg acgccgatgt
     2701 tgaacaaccc cgctttgaag cacaccgaca cggcgagggc ggcgaagatc aacggggttg
     2761 cgatcgccag gacagccccg atgctcgtgg ggtccttgaa ggcgaaccgc agcatcgcgc
     2821 cgtagacggt gaccgggctc tccccgtaga ggatgatgat cagcgacccc acggcgatcg
     2881 cgatcgcgaa ggccgacagg agcgcgagac cctggccgag cgcggtatcg agccagggcg
     2941 gccgcgaacg agggacgggc acggccggcg acgccgcgga catcagctcg cctccgccgt
     3001 ctcggtggcg ccgagcatcc cgatgccgag gtcctcgggc ggcgcgtcgg gggagtattc
     3061 acccgtgatc cgtccctcgt agatcgtgac gatgcgatcg gacagcgcgt agatctcgtc
     3121 gagctccgcg gagatcagga ggacgcctcg tccctcctct ttctgtcgca ggatctgtcg
     3181 ccagacgaac tcgatcgctc ctacgtcgag accgcgcgtc ggctgggacg caaccagcag
     3241 cttcgggctg gtgtccagct cacgagccag gaccagcttc tgctggttcc cgcccgagag
     3301 cgtgctggcg ttcacgttga tcgagcgggc gcgaacatcg aacgccttca cgagcctacg
     3361 ggccatgtct ttgatcttct ggatgaagag gaacccggtg cggccggaga acggtcggtc
     3421 gtctccgcga ccgaggaggc tgttctccca gagcgtcatg tccaggacga gcccctgctc
     3481 ctgccggtcc tcggggatgt acgcgacccc ggcgttccgg atgtcggcca cggaccaccc
     3541 ggcgatgtcc tggcccgcga gccggatcgt cccggcggcc ggctgacgaa gcccgatcag
     3601 cgcctccgcg agctcgcgct gcccgttccc ctcgaccccc gcgatcccga ggatctcacg
     3661 ctcgcggacc tgcagcgaga tgccgttcag cttcccctcg cccagcaacc cctccacttc
     3721 gagcaccggc gcgccgatct cgacctcggg cttgtccaga cggaacagca ccgggcgccc
     3781 gaccatcatc tcggcgagct tcgccttgga cgtctcggcc gggcgcgtct cgccgacgat
     3841 ctgcccacga cggagcacgg tgatgcgatc ggcgatgtcc agcacctcgt cgagcttgtg
     3901 gctgatgaag acgatggcct tcccgtcgtc gcgcagctgt cgcaggttct cgaacaggtc
     3961 cttggcctcg agtggtgtga ggacggccgt cggctcgtcc aggatcaaca ggtcgacgcc
     4021 tcgatagagc gccttcagga tctcgacccg ctgctcctcg ccgacggaca gatcctccac
     4081 gatcgcactc gggagcacat ggaagccgta cgtcttggcg agcgccgcga ctttctcatg
     4141 tgcggcgtcc atatccagga ccgcctcgcc ttcgtcgccg aggatcacgt tctccgtcac
     4201 ggtgaacgga tcgacgagga cgaagtgctg atgcaccatc ccgatcccgt gttcgatcgc
     4261 cacgcgtggt gacgtgatcc tcacgtcctc gccgttcacc acgatccgcc cttcgtccgc
     4321 cgggtacatc ccgtacagga cccgcatcaa tgtggacttc ccggcgccgt tctctccgat
     4381 cagtgcgtgc acctcgcccg cacgcacctc caacgaaacg tcgtcgttgg ccacgacgcc
     4441 cgggaaccgc ttcgtgatgt gctccatcac gacgaaaggc ggggcgcccg cgcgccccgc
     4501 ctctcgttga tcgctcatcg ggatctcacg atctccgacc tagggtgtgt cggggacggt
     4561 gatatcgccg tcgatgatct gctgcttcag gtcatcgtgc aaggtgatga tgtcgtcggt
     4621 catcagctcc gtgttgctct tcgagtagtc gacgccgccc tcggccatac cgaagacctg
     4681 gccgccagcc tccaggctgc cgtcgccgct ttgctcgatg gcgtctcgga ccgcggtgtc
     4741 cacgcgcttc agcatcgacg tcatgatgtg cggctgctgc tccgcgctca cgagctggta
     4801 ctggtcggag tcgaccccga tcgcgagtgc gtccgcggcc accgccgcgt tgaacaaccc
     4861 ggcgccggac gcgccggcgg cgtgatagat gatgcaggcg ccgtcgtcgt acatgccggt
     4921 cgacagtgcc tcacccttca cggcgtcgtt gaacgccgtc acgtcgtcgc cgatgtactg
     4981 cacgaggacc tcgatgccat cgttcacgtc cgcgacgccg gcctcgaagc ccgcctggaa
     5041 cttctcgatc agtgggccgg tctggccgcc caggaagcca acggtcgtgc agccgtcctt
     5101 gtcggcttgc gtagcggccg cggcgcccac caggtacgaa ccctcgtgct ccttgaacgt
     5161 caggtccgtc acgttcgtga ggccgtcgtt cgtcagcccg cacgcggtac cgcacgtcgc
     5221 gtagccgtcg atgatcgcga attgcgtgtc cgtgtagtcg ccggcgttct cattgatacc
     5281 cggtgagaac gcgaagccca ccgcgatcac gaggttgtga ccggcgtcgg ccaacgcgag
     5341 cacgttctcg tcgcggttcg atcccgtcgc gtccggctcg atcgattccg tgttctcctc
     5401 gcagacgagg ccctcgtcga tcgccgccgt gagaccggcg ttcgccgcgt cgttgaacga
     5461 cttgtccccg atcccaccga tgtcgtaggc gacgcccaca ctgaggtcgg cgttgcactc
     5521 cggcgcttcg ctgtcccctc cgggtggcga ttcggtgtca tcgccacagg ccgccccgac
     5581 gagcgcgagc acggccagca cagcgagcat ccgtagccat ttcggcatgc ttccctcccc
     5641 tatctggatc aaccgggccg cgcggcccga ccgcacggat tctccgggcg gagcgggggt
     5701 ccgtcaaacg ccagccggcg gaggccccgt gggagactac ggaacgggag gttccgggag
     5761 atgtccgagg acgaggacct gttccgagag ctccacgccc gcctcgccgc ctcgccgttc
     5821 cacacctggg cagggatgga agtcgtgagc gccgctcgcg gggcggtcac ggtcgcgatg
     5881 gagctccagg agcgtcacgc gaaccttcag ggcttcgttc acggtggcat gttgtcgatc
     5941 ctggcggata cggcgtgcgg gctgtcgatc cgctcggcga tggagcccgg acgtctccac
     6001 gtcaccgccg acctcgatat ccacttcctc gcgcctgcga agccggggag ggtcctcggt
     6061 cgtgggatcg ccgtgaaggt gggtcgatcc ctggcgttcg cagaggcctc gatcgaggac
     6121 ggcggaggcc ggctgctggc gaaggcgcag tcgcgcttct cggtcggggg gaccgagacc
     6181 tagtactcca cgtcgatctt ggccatggcc gcgggcagct gtgcgagggc tccctgcagg
     6241 ccgaggatca ggcccatgtt gccggcgatc ttgagcttgc ccgtcatgaa cgcggtgacg
     6301 ggcgagagct ctccgcgggc cagtcctgcg gccgagtcgt agctctgggt gatcgtggcg
     6361 tcctcaccct cgacgtcacc cacaccgagg tcgatccggc cgtcctcgat cgtgatccag
     6421 tagtgcgtgt cggcgccgcc accggtgatg acctgctgga tcgtggcgtg ctggcccgcg
     6481 gccgcccggc ggaactcctc gctctcgttg agctccgcct tgagcgcctg cgcccattcg
     6541 tctgacagga acttcacgga cacgatgacc ccctcggatg tcgatgactg gagtggctga
     6601 tgcggaggtc ccgggagtat aggagggccc tcactcgaag aagagcggat cggtgagatg
     6661 cagcacgtcg ttcaggaagc ggacggtccg cgtctcgccg tcgacgtcga cgctgttcag
     6721 gctcgtgttc tcggggagga agaacatctc ctgccgcagc ccgaggaggt cgccgacata
     6781 ggcgttgatc acgcctccgt gagcgaagac cagcacgttc tcgcttgggt ggtggtcgac
     6841 gatcgcatcg accgcggttc gcacccgggt gcggaacccg tccccgccct cggcacccgg
     6901 cgcgcgatgc cagatcgcgc gttggttccg gatgtgctgt acgacgtcgg gatcggagcc
     6961 catgatctcc tcgaacggct tgccctccca gccgccgatg tgcgcctcga cgagctcgtc
     7021 gtccacggtg acgcgggttc ctgccagccg cgcgaacgcc tccgcggtct cgttcgcgcg
     7081 ccggagcgga gaggagtaga cgacgaacgg atcgagatcc atcaccagga gcctgccggc
     7141 gaggagagcg gcctgctcgg caccgcgctc ggacagcggt gggtcccatt gctcgcctcg
     7201 ggcgctcgcc catcgctcgc cctgccagtc gaaatcggac tgaccgtgcc tgatcaggta
     7261 caggacgcgt tcggtcacgc gggcgcggag agcgtcgtcg aacggcgcgg cggggccgga
     7321 ggatgggtcg cccgccagtg atcggcatac ccctcgatat cctcacggat ccagatccgg
     7381 cccgacgcga gcgtggtgag aggctccggg aacgacccgc ggtcgatgta ggtgatgacc
     7441 cggcgcttgt cccagcccat gatctcggct gcctccgcga cgccgaccac cgccgggatc
     7501 acctcgatca tcagatccac gtcgtcgccc gtgaccttcc ggagctcctc gagacagccg
     7561 gctcggctcg tcgcggtgac ctcgatgtac gcggagacgt cgaggatgtg ggcgaaccat
     7621 ctccctccgg tctcccacat gcgggcccgg gcatgttcgc caaccgccat gtcgggatgc
     7681 tagcgcggcg gttcgacgac gtcgccgtct accacccgca gcagggcagg aggtagccgc
     7741 acgacgggca gcggtagtgc gcgtgctcgg gctccatcgg tgccccgcag gcgtcgcagg
     7801 gaagacgttt cgactccgcg ggaggcagac agcgatcggt ctcggccgga gcgtccatcc
     7861 tcgccacgat agacccgccg accgacgtca ctcgggaggc ggcgcccgat aggcgccgat
     7921 ccgccggagc tctttccgcc agcactccgt gtgccagtga cggcgctcgt cgggcgcgtc
     7981 gagcgggatg accacgaggt gccagatgcc cgcccgcacg acgtggtcgc agcccgggca
     8041 tcgatactcc ttctccccgc cgacatgtcg gacgtcgtag ccgggcagct cggcccaccg
     8101 gggggcggcc gaccgcggcc gcggagcctc ctgcggctgg aagtactcgg gatcgcggcg
     8161 gttcttcctc ggcatcagcc gtggtcccgg atgaacgccg cggcgtgcgg agccagggac
     8221 gcaccgacct cgcggggatc gcgtttccga ccacggacct cgaacgaatg gcccccaccc
     8281 tcgaacggga cgagcgtagc gagagcgccc agcttctcga cgacgctccg gaggagctcg
     8341 ggtgaggcga acgggtccga ggtcccctgc aggaagagca tcggggcctc gatccggtac
     8401 aggtgctcgt cccggatccg ctccggcttg cccggcgggt gcaggggata cccgaggaag
     8461 acgaggccgg ccgggtcgat gtcggcgtcg gctgcggcca tcgatgcgat cctgccgccg
     8521 agtgacttcc cgctcgccca caccggaccg ccggtggccc gatcgaccgc ggcctcgaac
     8581 gcggcacgcc aggcgtcacg gaggacgctc tcggggtccg gcgagcgcct gccgcgctcc
     8641 acgtagggga agttgaagcg gagagcggcg atcccttcct cgatgcatgc gcgggcgaac
     8701 ccgaccaaga aagggtggtc catcccggca ccggcgccgt gcgcgaccac gatggtcgcg
     8761 aacggcgacc gcggatgtgc atacaccgcc gcgatcggac ccttcacggt ctcgagacgc
     8821 agcgactcct cggtggcggc catcgcgtgc catgatgcac gcgatggccg gcaccctcca
     8881 tctggggacc tcgggtttct cgttcgacgg gtggaagcac ggcgtcttct atcccgaggg
     8941 gctgaagaac cgggagatgc tgtcctatta ctcgagccag c
//
LOCUS       DASUVE010000348         6651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1112229, whole genome shotgun sequence.
ACCESSION   DASUVE010000348 DASUVE010000000
VERSION     DASUVE010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112229"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..4873)
                     /locus_tag="VF036_07570"
     CDS             complement(68..4873)
                     /locus_tag="VF036_07570"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEX6265124.1"
                     /translation="MLARIGREAEAGVKSRPQDGAEGTIGPERAEAVAAFARALLRRL
                     ADDDLQAITTDELLALATSAFAFADSRGLEASAVRVFNAEPDIHGYRCHGTVIEVNTD
                     DSPFLVDSVSEELTARGLTIKRLLHPVIGAVRDEQGRLIGVRSGREAEHRESFMHFEV
                     DRALGHDVNAELETRVRRILRDVALVVRDFDPMQERVRHMIELARAAEVRYPSEVVGE
                     VARFLDWLLQLNFVLLGYREYELQETKDGRAIRAVPGSGLGILSDVEKSTFANLTLLS
                     DLAPDVRDRIEDGELLIYSKTQAYSTVHRRARMDYIGVRMISPDGKIVGEARLIGLFT
                     SKAYMEPAAKTPLLHHKLERLIAAEDLIPGSHDYKAVVTLFESFPKDELFQASAEELR
                     RLVAGLLQLEKHGGIRVLIRRDLYGRNVSVVVALPRDRFNADLRKALQGLFLERFNGT
                     TIDYHLSLGETESARIFFTIHVAPGKPIPDVRYEELEREVEILARTWEDDLLDALTRR
                     VGAGRALALMEEYAPRFPDYYKANDEWDLVVDDVVMLEKLEASTEGFLVGVGNESKGE
                     RLTRVKLYKTGGKVDLSDFMPILEALGLRVVEEVPTAIAGEGRVYIHDFGVLDSRGAV
                     LELGESADRVTDTIAAVWRGECDSDSLNRLVTLSALTWWEIRILRALRNYRMRVSARY
                     TENYRNDAMAAYPSISERLVRMFEARFDPVRAATDEEIDEIRQEIHRDLRGVSSLDQD
                     NILRHLLGTIEAIVRTNAYVPDRSFLSFKLLSERVPEMPKPMPLYEIWVYSTEMEAVH
                     LRAGKVSRGGIRWSDRREDFRTEVLGLMKAQKVKNAVIVPDGSKGGFVLKRTSITPEQ
                     IREEVAKQYVTFMEGLLDITDNLVKGEVVHPEGVRITDGPDPYLVVAADKGTAPLSDT
                     ANGVSERVGYWLGDAFASGGSYGYDHKELGITARGVWESVKRHFRELGTDPMTEPFTA
                     VGIGDMSGDVFGNGMLYTDRIRLVAAFDHRHVFLDPQPDPAISFAERKRLYETPGSSW
                     ADYDRSVLSEGAEVIDRTAKWVTLSARAREALAIPDDTPAEMTPADVIRWVLQAPVDL
                     LWNGGIGTYVKGANEGHTEVGDRANDPIRINGNQVHARVVGEGGNLGFTQRGRIEYAL
                     SGGRINTDFIDNSAGVDTSDREVNLKILLGLAIERGELTTDERNALMEASTDDVVRRV
                     LYDNYLQAQILSQEVAVAAERNEACEDLMLQLEAEGELDRLVEFLPTSDEMAERRGRG
                     EGLTRPEIALLVAYSKRSIKRDLLDADLPDAEYLEQDLARYFPETIVDRFRPMLAEHP
                     LRRELISTIVSNDVVNSQGVTFVSRLVAETGATPAEVVRAFRIARDVVGAVPRWDAVE
                     SLDGKIDPEIQNDLMIGVDRLVESTARWYLVRAPGLRLGEAVDQARTAFRELSSVIEQ
                     IGPEAWREQREREATRLTAEGIPDELARRHAFQDELVHGPDIISVSRATGRPVLEVAR
                     GFFLLGERLDIDWLEQRLAEQPAKTRWQRWAVHSMEDDLYTIRREIIETVLEHAGGRP
                     IDEAVDAFLEDRSAAYGRVQRFMRSLAMEGVTDLSQLTVALRQLRSLVS"
     gene            5138..6523
                     /locus_tag="VF036_07575"
     CDS             5138..6523
                     /locus_tag="VF036_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265125.1"
                     /translation="MARRVRIVVGEGRTAQQGLLRFVLEGEGYDVVGEAKSTSELSGV
                     IEANRPDVVVLDDGIGVVAVSMVHEVAPQAKVVLVWPSAVVPIGGDARVEPAKILRDL
                     GPAVEHVMAPVTALTETFVRPDWIDQVRKDPATLRNKLAGKETTKRPSVTRLQRRSQR
                     LHPRTKAAAAAAAAAAAAAERETREDAIPAPVVMLPVGPGGADVEPVLDIGGGTSAAR
                     PAEGSIDERSEWNRRLGTLALSGAAAVSALVLALALGGGRVNVGGVRGLVGTLPIVPG
                     FGQGAIVGRGPLTSGVLGAGGYAPGAFDPADLVPDVGTPGPTTDGGLDGGLANPDGSG
                     DPGTDPGTDPGTDPGTDPGTDPGPDPGPDPGPDPGPGDGNDDDDQGNDDDDDDQGNGN
                     DDDDDDDDEDGDDGGHDDDDGDDDGLPGKSGEHNPHGGPPGHLGTTPAHGPGNGVGNG
                     QGQGQGHKHKH"
BASE COUNT         1058 a         2290 c         2254 g         1049 t
ORIGIN      
        1 atcggggtcg tcaaggcacg tggtctcctg tgggtgacca cgatcttcta cggcggctga
       61 cctcgcctca ggacacgagc gaacggagct ggcggagcgc gaccgtgagc tgcgagaggt
      121 ccgtgacccc ctccatcgcg agtgaccgca tgaaccgctg caccctcccg taggcggcgc
      181 tccgatcctc gaggaacgca tcgacggctt catcgatcgg gcgtccgccc gcatgttcga
      241 gcacggtctc gatgatctcg cggcggatcg tgtacagatc gtcctccatc gagtgaacgg
      301 cccatcgctg ccaccgagtc ttcgccggct gctcggcgag ccgctgctcg agccaatcga
      361 tgtccagccg ctctccgagg aggaagaacc cgcgcgccac ctcgagaacc ggccgtccgg
      421 tggcgcgcga caccgagatg atgtccgggc cgtggaccag ctcgtcctgg aacgcatgtc
      481 tgcgcgccag ctcgtcgggg ataccttcgg cggtgaggcg cgtcgcctcc cgctcgcgct
      541 gctcgcgcca cgcctcagga ccgatctgct cgatcaccga cgacagctcg cggaacgcgg
      601 tcctcgcttg atcgacggcc tcgccgagcc ggagccccgg cgcgcgtacg aggtaccagc
      661 gcgcggtcga ctcgaccagc ctgtcgaccc cgatcatcag gtcgttctgg atctccgggt
      721 cgatcttgcc gtcgagggac tccaccgcgt cccatcgcgg cacggcgccc acgacgtcgc
      781 gggcgatccg gaacgcgcgc acgacctcgg ccggggtcgc ccccgtctcc gcgacgagcc
      841 gcgacacgaa cgtgactcct tgggagttca cgacgtcgtt cgagacgatc gtcgagatca
      901 gctctcgccg caacggatgc tcggccagca tcggccggaa ccggtcgacg atggtctccg
      961 ggaagtaccg cgccaggtcc tgctcgaggt actcggcgtc ggggaggtcc gcatcgagga
     1021 ggtcacgctt gatcgagcgc ttcgagtacg cgaccagtag cgcgatctcc ggccgtgtga
     1081 gtccctcgcc gcgacccctt cgctccgcca tctcgtccga ggtgggcagg aactcgacga
     1141 gacgatccag ctccccttcc gcctcgagct gaagcatcag gtcctcgcac gcctcgttcc
     1201 gctcggcggc gaccgcgacc tcttgcgaga ggatctgcgc ctgtaggtag ttgtcgtaga
     1261 gcacacgacg cacgacgtcg tccgtggagg cctccatcag ggcgttgcgt tcatccgtcg
     1321 tcagctctcc tcgctcgatc gccagaccga ggaggatctt gaggttcacc tcccggtccg
     1381 aggtgtcgac gccggcggag ttgtcgatga agtccgtgtt gatccgtcca ccgctgagcg
     1441 cgtactcgat ccgcccccgc tgagtgaagc cgaggttgcc accctccccc accacgcggg
     1501 catggacctg gttcccgttg atccggatgg ggtcgttggc gcggtcgccc acctcggtgt
     1561 ggccctcgtt cgcccccttg acgtacgtgc cgatcccacc gttccagagc aggtcgacag
     1621 gtgcctggag cacccagcgg atcacgtccg cgggggtcat ctcggccgga gtgtcgtcgg
     1681 ggatcgcgag ggcctcgcgc gcccgggccg acagcgtcac ccacttcgcc gtgcggtcga
     1741 tcacctccgc cccctcggag agcacgctcc tgtcatagtc ggcccacgag gatcccggcg
     1801 tctcgtacag gcgcttacgc tcggcgaacg agatcgccgg atccggctgc ggatcgagga
     1861 acacgtgtcg gtggtcgaac gcggcgacca acctgatccg atccgtgtag agcatgccgt
     1921 tgccgaagac gtccccggac atgtcaccga tcccgaccgc cgtgaacggc tccgtcatcg
     1981 ggtccgtgcc aagctcgcgg aagtgccgct tgaccgattc ccacacaccg cgggcggtga
     2041 tgccgagctc cttgtggtcg tagccgtagg agcctccgga ggcgaacgcg tcaccgagcc
     2101 agtacccgac gcgctcgctc acgccgttgg ccgtgtccga caggggagcg gtgcccttgt
     2161 cggcggcgac caccaggtag gggtcggggc catccgtgat ccgcacccct tcggggtgca
     2221 cgacctctcc cttcacgagg ttgtcggtga tgtcgagcag tccctccatg aacgtgacgt
     2281 actgcttcgc cacctcttcc ctgatctgct ccggggtgat cgaggtccgc ttgaggacga
     2341 acccaccctt ggagccgtcg ggaacgatca cagcgttctt gaccttctgg gccttcatca
     2401 gtccgaggac ctcggtgcgg aagtcctccc tgcggtcgga ccaacggatc cccccgcggg
     2461 acaccttgcc tgcccggaga tggaccgcct ccatctccgt ggagtacacc cagatctcgt
     2521 agagcggcat cggcttcggc atctccggca cgcgctccga gaggagcttg aagctcagga
     2581 acgatcggtc ggggacatac gcgttcgtcc gcacgatcgc ctcgatcgtc ccgagcaggt
     2641 gtcgcaagat gttgtcctgg tcgagggagg agacgcctcg caggtcgcgg tggatctcct
     2701 gccggatctc gtcgatctcc tcgtccgtgg cggcacgcac gggatcgaaa cgggcttcga
     2761 acatccgcac gagccgttcc gagatcgacg ggtaggccgc catcgcgtcg ttccggtagt
     2821 tctcggtgta ccgggcggag acgcgcatcc gatagttccg gagcgcgcgg aggatcctga
     2881 tctcccacca ggtgagggcc gacaacgtca cgaggcggtt cagcgagtcc gaatcgcact
     2941 cccctcgcca cacggccgcg atcgtgtccg tgacgcgatc cgccgattcc ccgagctcca
     3001 gcaccgcgcc ccgtgagtcg agcaccccga agtcgtggat gtacacccgt ccctcgcctg
     3061 cgatcgcggt cggcacctcc tcgacgaccc ggagtccgag cgcttcgagg atcggcatga
     3121 agtccgagag atcgaccttg ccgccggtct tgtagagctt cacccgcgtc aggcgttcgc
     3181 ccttcgactc gttgccgacg ccgacgagga acccctcggt cgacgcctcg agcttctcga
     3241 gcatcacgac gtcgtcgacg accaggtccc actcgtcgtt ggccttgtag tagtccggga
     3301 accgcggcgc gtactcctcc atcagggcga gcgctcgacc cgcgccgacg cgccgcgtga
     3361 gcgcgtcgag gaggtcgtcc tcccaggtgc gcgctaggat ctcgacctct cgttcgagct
     3421 cctcgtagcg cacgtcgggg atcggcttgc cgggggcgac atggatcgtg aagaagatgc
     3481 gcgccgactc cgtctccccc agggagaggt ggtagtcgat cgtcgtgccg ttgaagcgct
     3541 cgaggaagag gccctggaga gccttgcgga ggtccgcgtt gaaccgatcg cgcggcaggg
     3601 cgaccacgac cgacacgttg cggccgtaga ggtcgcggcg gatcaggacg cggatgcccc
     3661 cgtgcttctc gagctgcagg agcccggcca ccaaccgccg cagctcctcg gcggaggctt
     3721 ggaagagctc gtccttcggg aaggactcga agagggtgac caccgctttg tagtcgtgcg
     3781 atccggggat caggtcctcc gcggcgatca accgctcgag cttgtggtgc agcaacggcg
     3841 tcttcgcggc cggctccatg tacgccttgc tcgtgaagag gccgatcagc cgggcctcac
     3901 ccacgatctt gccgtcgggt gagatcatcc ggaccccgat gtagtccatg cgggcgcggc
     3961 ggtggacggt cgagtacgcc tgggtcttcg agtagatcag gagctccccg tcctcgatcc
     4021 gatcccggac atccggtgcg aggtccgaga gcagcgtgag gttcgcgaac gtcgacttct
     4081 cgacatccga gaggatcccg agcccgctgc ccggcaccgc acggatcgcg cgtccgtcct
     4141 tcgtctcctg gagctcgtat tcgcggtagc cgagcagaac gaagttcagc tggaggagcc
     4201 agtcgaggaa cctcgccact tcgccgacga cctccgacgg gtaacggacc tcggcggcgc
     4261 gtgcgagctc gatcatgtgc cgcacgcgct cctgcatcgg atcgaagtcc cggaccacga
     4321 gcgcgacatc tcggaggatg cggcgcacgc gcgtctcgag ctcggcgttc acgtcgtggc
     4381 cgagagcacg gtcgacctcg aagtgcatga acgactcgcg atgctcggct tcccgcccgg
     4441 accggacgcc gatcagtcgc ccctgctcgt cgcggacagc cccgatcacg gggtggagca
     4501 gacgcttgat cgtgagaccg cgcgccgtga gctcctccga gaccgagtcg acgaggaagg
     4561 gcgagtcgtc ggtgttgacc tcgatgaccg tgccgtggca tcggtacccg tggatgtcgg
     4621 gctcggcgtt gaagacgcgg acggcggatg cctcgaggcc gcggctgtcc gcgaacgcga
     4681 aggcagaggt ggcgagcgcg agcagctcgt cggtcgtgat cgcctggagg tcgtcatcgg
     4741 ccaagcgtcg caggagcgct cgcgcgaacg cggcgaccgc ctcggctctc tcgggcccga
     4801 tcgttccctc ggcgccgtcc tgcggccggg acttgacacc ggcctcggcc tcgcgcccga
     4861 tccgagccag catctcttcg atcttgggat ggagctcgga gggggtggtg gggggcgctg
     4921 actgttcgat cgtgtccgac actggcgttc gcgaccctcc ggggctgacc gctccattgt
     4981 gtcaggtcat ccggccgaga tggggacacc gagtgcgacg gccggtaccg aaggtcgtac
     5041 gggctgtact actcctttcg gcacgaaaac ccccagaagg ctgaggccga gaatccgtgc
     5101 gtgaagtacg ctcggcgggg catcctgggg aggcacattg gcgcgacggg tacgcatcgt
     5161 cgtcggcgag ggtcgaacgg cgcaacaagg gctccttcgc ttcgtcctcg agggcgaggg
     5221 atacgacgtc gtgggggagg caaagagcac ctccgagctc tccggcgtga tcgaagccaa
     5281 ccgcccggat gtggtcgtcc tcgacgacgg tatcggcgtg gtcgccgtct cgatggtcca
     5341 cgaggtggcc cctcaggcga aggtggtcct cgtgtggccg tcggccgtcg tcccgatcgg
     5401 cggggatgcc cgcgtggagc ccgccaagat cctccgggac ctcggcccgg cggtggagca
     5461 cgtgatggcg ccggtcacgg cgctcacgga gacgttcgtg cggccggact ggatcgacca
     5521 ggtccgcaag gacccggcca cgctgcggaa caagctcgcc ggcaaggaga cgacgaagcg
     5581 ccccagcgtc acccgcctgc agcggcgcag ccagcgcctc catcccagga ccaaagcagc
     5641 agccgcagcc gcagccgcag ccgcagccgc ggcggagcgg gaaacgcgcg aggacgcgat
     5701 cccggcgccg gtcgtgatgc tcccggtcgg gcccggcgga gcagacgtcg agcccgtgct
     5761 cgacatcggc ggaggcacgt ccgctgctcg tccggccgag gggtcgatcg acgagcggtc
     5821 ggagtggaac cggcgcctcg gcaccctcgc gctgagcggt gcggcggcgg tgagcgctct
     5881 ggtgctcgcg ctcgccctcg gcggtggcag ggtgaacgtg ggtggggtcc gcggcctggt
     5941 cggcactctc ccgatcgtcc ccggcttcgg gcagggtgcg atcgtcggtc gcgggccgct
     6001 cacgtcgggc gtgctcggag caggtgggta cgcgccaggc gcgttcgatc ccgccgacct
     6061 cgtgccggac gttggcaccc cgggccccac gaccgacggt ggcctcgatg ggggcctcgc
     6121 gaaccccgac ggtagcgggg atcccgggac cgacccgggc accgaccccg gaaccgatcc
     6181 tggaaccgac cccggaaccg atcctggacc cgatcctgga cccgaccctg gacccgatcc
     6241 tggacccggc gacgggaacg acgacgacga ccaggggaac gacgatgatg acgacgacca
     6301 ggggaacggg aacgacgacg acgacgatga tgacgacgaa gacggggacg acggcgggca
     6361 cgacgacgac gacggggacg acgacggcct cccggggaag agcggggagc acaacccgca
     6421 cggtggccct cccggtcacc tggggaccac gcccgctcac ggtcccggaa atggcgtggg
     6481 caacggtcag ggccaagggc agggccacaa gcacaagcac tgaccgaggg agcctcgatc
     6541 cagaagccta ggctgagccc atgggttcca acccctacgg ctcgctcgaa cccggcacac
     6601 gggatccgat cccgaaacca ctgttccccg aggtgagctc caccggagcg a
//
LOCUS       DASUVE010000349         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1112512, whole genome shotgun sequence.
ACCESSION   DASUVE010000349 DASUVE010000000
VERSION     DASUVE010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112512"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VF036_07580"
     CDS             <1..586
                     /locus_tag="VF036_07580"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX6265126.1"
                     /translation="RTPPTILERLIGAAAASHLGDLARGVDDRRVEPYEQPKSIGHEE
                     TFDRDLDDEDEILRELLALSGRVAARLREDGYRARTVTLKVRLANFTTLTRSRTQPDP
                     TDLGGEIHRTVSELYRHLPGERRRVRLLGVQAAGLDSAGAHQLALLRSERWDDVERTV
                     DRIERRFGTGAAGPASLLDRSRTRSVPLDRDPSL"
     gene            628..990
                     /locus_tag="VF036_07585"
     CDS             628..990
                     /locus_tag="VF036_07585"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HEX6265127.1"
                     /translation="MPLNDHEQKILEEIERRLSEEDPRLVEQVTRTDLHTHAWGRMKL
                     AIAGFVVGSLMLLLVGVSVWIAAAGFVAMTLSAVLAYRYAGRLGDDTVAPGAFGRIAS
                     RVAGRLRPPPSAPPSDER"
     gene            1212..1709
                     /locus_tag="VF036_07590"
     CDS             1212..1709
                     /locus_tag="VF036_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6265128.1"
                     /translation="MEGFTAHQACKFTGCSPRQLRYWDEIGLVEPSVQGTGGRPGVPR
                     LYSFRDLVALRVVKSLLDGGMSLQRVRRSWEFLTKKAGLTKHLSEVKLVTDGQSIFKI
                     CRRDGEVIDALRDGQMAFFVAIDDIATGVETKVRQFEEDRERFVRALRAAAATTATGQ
                     SAAAR"
     gene            complement(1721..>3679)
                     /locus_tag="VF036_07595"
     CDS             complement(1721..>3679)
                     /locus_tag="VF036_07595"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEX6265129.1"
                     /translation="TLRAALDAAALVGEQARIQPAPAEPIDPLLLAAVVSLWAAVFSA
                     HALAFRAGSPLLGLVPPVALVAFADTVLEEFVKPLYGLAFLAAALLVVFADGLARVQG
                     WGPVWASGRRGIAATAGRGARRLAFTALGAALLSPILVPGFGSKGLVDFGTPAADRIA
                     IDPFVSVESALTQTEPVEVLRVTTTHPGYIRLVTVPEFDGVGWRPAEGSEAVPLVPGA
                     ELPDPGRGEETTARIEIMSDLGQRYLPAPSPIGSIQGITDRTVLYDGDLWNAFVDRPL
                     SEGDIYEVTTYRPRVTAEALRNIPYFNTLEGGRYTDVPDDLDERIRTIALDWVGEADN
                     VFDRVVAIEERLHSSGEFGYQVDTTLRSGTSSILEFLTETKAGFCQQFATSMAAMLRT
                     IGIQSRVVVGYATGLPVAPGEYSFTTDVAHAWVEVWFPGWGWMPFEPTPTRTNAVSYV
                     SSGDAPCPGQDCAGETPPRGTGGSATQNREATTVEDLRPGNGPRGATGDPGTIDIADD
                     ASPISARMALLLGAIVVGLVLLFVPPVRALRRRIRLRRAGDEPRRLILATYEVFTERA
                     TGIGLGRDPGETLNEYRTKVMETGYLSNGHLDRLTGLASAAAYSPHAPSAEQAREAGS
                     AADTAIREIRRSVGPTAWFLGLYRGRWT"
BASE COUNT          551 a         1285 c         1301 g          542 t
ORIGIN      
        1 gaggacgccc ccgacgatcc tcgagcgtct gatcggcgcc gcggcggcga gccacctagg
       61 cgatctggcg cgcggggtcg acgaccgccg ggtcgagccc tacgagcaac cgaagtccat
      121 cgggcacgag gagacgttcg atcgggacct cgatgacgag gacgagatcc tgcgggagct
      181 gctggcgctc tcggggcggg tggccgctcg tctccgcgag gacggctatc gtgcgcggac
      241 ggtcacgctg aaggtccggc tcgcgaactt cacgacgctc acccgttcgc gcacgcagcc
      301 cgatcccacc gacctcggtg gagagatcca ccggaccgtg tccgagctgt atcgccacct
      361 ccccggggag agacgacgtg ttcggctgct cggggtccag gcagcgggat tggactcggc
      421 gggcgcgcac cagctggcgc tgctgcgctc cgaacgctgg gacgacgtgg agcgcaccgt
      481 ggaccggatc gagcgtcgct tcgggaccgg tgcggcgggg cccgcgagcc tcctcgaccg
      541 atcgcgcacc cgttcggtac cactcgatcg ggacccctct ctataatcgg aggggaacgg
      601 ggtcgccccc cgcgggccgg aggtgccatg ccgctcaacg atcacgagca gaagatcctg
      661 gaggagatcg aacggcggct ctcggaagag gatccgaggc tcgtcgagca ggtcacccgc
      721 acggacctcc acacgcatgc ctggggtcgg atgaagttgg cgatcgcggg gttcgtcgtc
      781 ggatcgctca tgctcctcct cgtcggcgtc tccgtctgga tcgcggccgc ggggttcgtc
      841 gcgatgaccc tgtctgcggt gctcgcctac cggtacgccg gtcgtctcgg cgacgacacc
      901 gtcgccccgg gcgcgttcgg aaggatcgcg tcgcgcgtcg ccgggcggct ccgcccgccg
      961 ccgtcggcac ctccgtcgga cgagcgatag cgcgcgcgca ccgacgcgtg cgcggacccc
     1021 ccgcgaacgt cgcgtagatc acaccgtctg accactgcgc ttgccacgac ttcgtggatg
     1081 ggtgtagaac gtgcattcct tcccctgggt gcgagtgcgt cgccggaggg cgggttcccc
     1141 gggagggccc gtgacgttcg cgccggatgg ttcgggttcg aagttgtcgt gatcgaaggg
     1201 gcgcgcgtcc gatggagggt ttcaccgcgc atcaggcatg taagttcacg ggttgctcgc
     1261 cacgtcagct gcgctactgg gacgagatcg ggctcgtcga gccctcggtg cagggcaccg
     1321 gtggacgccc cggcgtaccg cgcctgtact ccttccgaga tctggtggcg ctgcgggtcg
     1381 tgaagtcact gctcgacggg gggatgtcac tgcaacgggt ccgccgttcg tgggagttcc
     1441 tcaccaagaa ggccggcctc acgaaacacc tctccgaggt gaagctcgtg acggacgggc
     1501 aatcgatctt caagatctgc cggcgcgatg gcgaggtgat cgacgccctc cgcgacgggc
     1561 agatggcgtt cttcgtcgcc atcgacgaca tcgcgacggg cgtcgagacg aaggtccgtc
     1621 agttcgagga agaccgcgag cggttcgtac gagcgctgcg cgccgctgcg gccaccacgg
     1681 cgacgggtca gtcggccgcc gcccgctagg cggaccgtcc tcaggtccag cggccgcgat
     1741 agaggccgag gaaccacgcc gtcggcccga cggaccgccg gatctctcgg atcgccgtgt
     1801 ccgcggcgct cccggcctcc cgtgcctgct ccgccgaggg cgcatgagga gagtacgcgg
     1861 ccgccgacgc gaggccggtc agccgatcga ggtgcccgtt cgacaggtac ccggtctcca
     1921 tcaccttcgt gcggtactcg ttcagggtct ctcccgggtc gcgaccgagc ccgatcccgg
     1981 tggcccgctc cgtgaacacc tcgtacgtcg ccaggatcag ccgtcgcggc tcgtccccgg
     2041 cccggcgcag acggatccga cgccggagag cccgcacggg cggcacgaac aggagcacga
     2101 gcccgacgac gatggcgccg aggaggagcg ccatccgcgc gctgatcggc gaggcgtcgt
     2161 cggcgatgtc gatcgtgccg ggatcgccgg tggctccgcg agggccgttc cccggtcgca
     2221 gatcctcgac cgtggtcgcc tcgcggttct gggtggcgct tcccccggtt ccgcgggggg
     2281 gagtctctcc cgcgcagtcc tgcccgggac acggggcgtc cccggaggac acgtagctca
     2341 ccgcgttcgt tcgcgtcggc gtgggttcga acggcatcca gccccacccc gggaaccaca
     2401 cctccaccca cgcatgggcg acatcggtgg tgaacgagta ctcgcccggt gcaaccggca
     2461 ggcccgtggc gtagcccacg acgacccggg actggatgcc gatggtgcgg agcatcgcgg
     2521 ccatcgaggt ggcgaactgc tggcagaagc cggctttcgt ctcggtcagg aactcgagga
     2581 tcgagctcgt tcccgaccgg agcgtggtgt cgacctgata gccgaactcg ccggagctgt
     2641 gcaggcgctc ctcgatcgcg acgaccctgt cgaagacgtt gtcggcttcc cccacccagt
     2701 cgagcgcgat cgtccggatc cgctcgtcca agtcgtccgg gacatccgtg tagcgaccgc
     2761 cctcgagcgt gttgaagtac gggatgttcc ggagcgcctc ggccgtcact cgcgggcgat
     2821 acgtggtcac ctcgtagatg tcgccttcgc tcaacggtcg atccacgaac gcgttccaca
     2881 ggtccccgtc gtagaggacg gtccgatcgg tgatcccttg gatcgaaccg atcggggagg
     2941 gagcagggag gtatcgctgc cccaggtcgc tcatgatctc gatccgggcg gtcgtctcct
     3001 cgcctcggcc cggatccggg agctcggcgc cgggaacgag cggcacggcc tcggacccct
     3061 ctgcgggtcg ccagcccaca ccgtcgaact cgggcacggt gacgagccgg atgtagcccg
     3121 gatgggtcgt cgtgacccgg agcacctcga caggttcggt ctgcgtcagt gcggactcga
     3181 ccgagacgaa cgggtcgatc gcgatgcggt cggctgccgg tgtcccgaag tcgacgagcc
     3241 ccttggaccc gaaccccggc accaggatcg gtgagagcag cgccgctccc agcgccgtga
     3301 acgcgaggcg gcgcgctccc cggccggccg tggcggcgat cccgcgccga ccggacgccc
     3361 acaccggtcc ccatccctgc acccgcgcga gaccgtccgc gaacacgacg aggagcgccg
     3421 cggcgaggaa cgccagtccg tagagcggct tcacgaattc ctcgagcacc gtgtcggcga
     3481 acgcgaccag cgcaacgggc ggcaccagcc ccaggagcgg actccccgcg cggaacgcca
     3541 gagcgtgcgc ggagaacacc gcggcccaca gcgaaacgac cgccgccagg aggaggggat
     3601 cgatcggctc cgccggcgct ggctggatcc tcgcctgctc gcccacgagc gcggcggcgt
     3661 cgagggcggc ccggagcgt
//
LOCUS       DASUVE010000350         2897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_737334, whole genome shotgun sequence.
ACCESSION   DASUVE010000350 DASUVE010000000
VERSION     DASUVE010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737334"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2235
                     /locus_tag="VF036_07600"
     CDS             <1..2235
                     /locus_tag="VF036_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX6265130.1"
                     /translation="KKVLELSLREALQLGHNYIGTEHILLGLIREGEGVAAQVLQKLG
                     ADLNRVRQTVIQLLSGYTAGKGEQVTGSQEGGQGSMVLDQFGRNLTQLARDGKLDPVI
                     GREKEIERVMQVLSRRTKNNPVLIGEPGVGKTAVVEGLSQDIVREEVPETLKGKQIYT
                     LDLGALVAGSRYRGDFEERLKKVLKEIKTRGDIVLFIDELHTLVGAGAAEGAIDAASI
                     LKPMLARGELQTIGATTRDEYRKHLEKDAALERRFQPIVVEEPTVSHTIEILKGLRDR
                     YEAHHRVSFTDDALVAAANLADRYIADRSLPDKAIDLIDEAGSRTRIRRMTAPPDVRE
                     VDEKIAEVRLRKESAIDAQDFEGAASLRDEERQLQEERQRREQRWKAGEMDVLSEIGE
                     EEIAEVLSVWTGIPVFKLTEEETEKLLRMEEEIHKRIISQHESISAVSRAMRRTRSGL
                     KDPRRPSGSFIFLGPSGVGKTELSKALAEFLFGDEDALIQLDMSEYMEKHTVSRLIGS
                     PPGYVGYEEGGQLTEAVRRKPFAVVLFDEIEKAHPDVFNTLLQILEDGHLTDAQGHKV
                     DFKNTIIIMTSNLGTRDIAKGVGIGFTAARDDAVTYTKMKENVTEELKRSFRPEFLNR
                     IDEVIVFHSLTPDDVKQIVDLLMRRVEEQLKSKDVDIVLTDDAKTLLAERGYDKALGA
                     RPLRRTIQQLVEDPLAEKLLYKEFRAGETIVVDVRDGEITFEHGAGIMPPDTPPVELA
                     GSTE"
     gene            complement(2346..>2897)
                     /locus_tag="VF036_07605"
     CDS             complement(2346..>2897)
                     /locus_tag="VF036_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265131.1"
                     /translation="GAKDAVSWSPTGDELAYAFNGEIYAMSADGSNKRRLTNSPDGMG
                     SFAPTWAPDGIAIAFWRGTSVFEGDGDAPEDSEIYVVGVGAGEAFRLTHDDHASFQPA
                     WSPDSSQIAYRRTNPDDIVVMNADGTGGHAVTPAALNPWSPAARCSKSPCSTSPPERS
                     SGSTCTSRRRTTSRSGSPIQPFW"
BASE COUNT          524 a          975 c          962 g          436 t
ORIGIN      
        1 aagaaggtcc tcgagctctc gctccgcgaa gcgctgcagc tcggacacaa ctacatcggc
       61 accgagcaca tcttgctcgg gctgatccgc gaaggcgaag gggtcgctgc acaagtgctc
      121 cagaagctcg gcgccgacct caaccgcgtc cgccagaccg tgatccagct cctgtccggc
      181 tacacggcgg gcaagggcga gcaggtcacc ggctcccagg agggcggtca gggctcgatg
      241 gtcctcgacc agttcggtcg gaacctcacc cagctcgcgc gcgacggcaa gctcgacccc
      301 gtgatcggcc gtgagaagga gatcgagcgg gtcatgcagg tcctctcgcg ccggaccaag
      361 aacaaccccg tgctgatcgg tgagccgggc gtcggcaaga ccgccgtcgt cgagggcctg
      421 tcgcaggaca tcgtccgtga agaggtgccc gagacgctga aaggcaagca gatctacacg
      481 ctggacctcg gcgcgctggt cgcgggctcc cggtaccggg gcgatttcga ggagcgcctg
      541 aagaaggtcc tcaaggagat caagacccgt ggcgacatcg tgctgttcat cgacgagctg
      601 cacacgctgg tcggtgcggg tgccgcggag ggcgcgatcg acgccgccag catcctcaag
      661 ccgatgctcg cgcgcggtga gctgcagacg atcggcgcca ccacgcgtga cgaataccgc
      721 aagcacctcg agaaggacgc ggcgctcgag cggcggttcc agccgatcgt ggtcgaggag
      781 cccaccgtct cccacaccat cgagatcctg aagggcctcc gggatcgcta cgaggcccac
      841 caccgggtga gcttcaccga cgacgccctc gtggccgccg cgaacctcgc ggaccgctac
      901 atcgcggatc gttcgctgcc ggacaaggcg atcgacctga tcgacgaagc cgggtcccgc
      961 acgcggatcc gtcggatgac ggcgccgccc gacgtgcgtg aggtcgacga gaagatcgcc
     1021 gaggtccgtc tgcggaagga gtcggcgatc gacgcgcagg acttcgaggg cgcggcatcc
     1081 ctccgcgacg aggaacggca gcttcaggag gagcgccagc gccgcgagca gcgatggaag
     1141 gccggcgaga tggacgtcct gtcggagatc ggcgaggagg agatcgccga ggtgctctcc
     1201 gtctggaccg gcatccccgt cttcaagctc accgaggagg agacggagaa gctcctccgc
     1261 atggaggaag agatccacaa gcgcatcatc agccagcacg aatcgatctc cgccgtttcc
     1321 cgcgcgatgc gccggacgcg gagcggactg aaggatcccc ggcgcccttc gggctcgttc
     1381 atcttcctcg gaccatcggg cgtcgggaag accgagctgt ccaaggccct ggccgagttc
     1441 ctcttcggcg acgaggacgc gctgatccag ctcgacatgt ccgagtacat ggagaagcac
     1501 acggtctccc gcctgatcgg ctcgcccccc ggctacgtcg gctacgagga gggcggacag
     1561 ctcaccgagg ccgttcgtcg caagccgttc gccgtggtgc tgttcgacga gatcgagaag
     1621 gcgcacccgg atgtcttcaa cacgctgctg cagatcctgg aggatgggca tctcacggat
     1681 gcacagggcc ataaggtgga cttcaagaac acgatcatca tcatgacctc gaacctcggc
     1741 acccgggaca tcgccaaggg cgtggggatc gggttcacgg ccgcccgcga cgacgccgtc
     1801 acgtacacga agatgaagga gaacgtaacc gaggagctca agcgctcctt ccggcccgag
     1861 ttcctgaacc gcatcgacga ggtcatcgtg ttccactcgc tcacccccga cgacgtcaag
     1921 cagatcgtcg atctgctgat gcgccgggtg gaggagcagc tgaagtccaa ggacgtcgac
     1981 atcgtcctca ccgatgacgc caagaccctg ctcgccgagc gtgggtacga caaggccctg
     2041 ggcgctcgac cgctccgccg cacgatccag cagctggtgg aggacccgct cgccgagaag
     2101 ctcctctaca aggagttccg cgccggagag acgatcgtcg tcgacgtgcg cgacggcgag
     2161 atcacgttcg agcacggcgc cggcatcatg ccgcccgaca caccgcccgt cgagctcgcg
     2221 ggctcgaccg agtaggtcgc gcccgcgtca cgcgccgcgt ctccggacgc ggcgggaagg
     2281 ccgaagcccg gtccgtcgga gaccgggctt ctcggcatct cagtccgacc gctattcctg
     2341 gcggttcacc agaagggttg tatcggtgat ccactgcggg acgtggttct ccgtctcgac
     2401 gtacatgtcg agccgctgga tctctccggt ggtgaggtcg agcacggcga cttcgagcag
     2461 cgggcggccg gcgaccacgg gttcaaggcc gctggagtca cagcgtggcc gcccgtgccg
     2521 tcagcgttca tcaccacgat gtcgtccggg ttggtccgcc gatacgcgat ctgcgaactg
     2581 tccggcgacc acgcgggctg gaagctcgcg tgatcgtcgt gggtcagccg aaacgcctcg
     2641 ccggcgccga cacccacgac atagatctcc gagtcctccg gggcgtcgcc gtcgccctcg
     2701 aacacgctcg tgcctcgcca gaacgcgatc gcgatgccgt cgggtgccca tgtcggagcg
     2761 aaactgccca tcccatccgg gctgttggtc agccgccgct tgttggatcc gtcggcgctc
     2821 atcgcgtaga tctcgccgtt gaaggcgtac gcgagctcgt cgcccgttgg ggaccagctc
     2881 acggcgtcct tggcgcc
//
LOCUS       DASUVE010000351         2866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_738030, whole genome shotgun sequence.
ACCESSION   DASUVE010000351 DASUVE010000000
VERSION     DASUVE010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738030"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VF036_07610"
     CDS             <1..724
                     /locus_tag="VF036_07610"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX6265132.1"
                     /translation="TCDCASFDRLGVLGEAASGAGELIWIDHHRSNDGIGSVRLVDPD
                     ASSTCEMVFRLIETMGGDFDRDVAVCLYAGLVTDTGRFQYQATTPETLRIAARLREED
                     FDHAGLVRAMYEDNDPGFLRLLGTALGRMTEVPEASVVWTYLTQADLADAGVAAGETD
                     DVIDVLRTARDVDVAAVLKQQRDGRFKVSVRSRGGHDLSAVASAFGGGGHRLAAGYTS
                     EHGPEGTIERLVAALRPAPVPS"
     gene            721..1572
                     /gene="truB"
                     /locus_tag="VF036_07615"
     CDS             721..1572
                     /gene="truB"
                     /locus_tag="VF036_07615"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX6265133.1"
                     /translation="MTAQGPEGVLLLDKPAGITSHDVVDSVRRTLATRKVGHAGTLDP
                     MATGLLVIGVGRATRLLRFLGDLLKTYEGTLRLGVETDTLDADGAVVRESPVDLTEGR
                     LAGAMQAMVGDSLQAPPAYSAVKVGGRKLYEAARAGERLEAKSRPIRVEAFELLSFDP
                     PDASFRVVCSGGTYVRVLAADVGAAVSTGAHLMTLRRTAIGPYRVEDAARPDSVGDPF
                     PIEAAVRHLPRLDLDADEAEAASYGRPLGPADLEGPYGVFGPDGRLIGVYEDDGPRAR
                     PHVILAG"
     gene            1594..2532
                     /locus_tag="VF036_07620"
     CDS             1594..2532
                     /locus_tag="VF036_07620"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX6265134.1"
                     /translation="MDIVRGLDALPLPDGPSVVTIGFFDGVHLGHQEVFRRTVEAARE
                     RDARSVAVTFDRHPRETLTPGEEPRLLTTVERKAALIDACGIDVLVVLAFTQEFSLVP
                     AETFVRDVLVGRLHAVHTRVGANFTFGHRAVGTVTTLREVGEPLGMTADDVDLFELEG
                     RVVSSSSIRDALASGDLAWPRTALGRRYGVDGEVVAGAGRGKGLGYPTANLRTWPRLL
                     LPGQGIYAGTAELFDGSRYPAAIDVGTNPTFGTEPLHVEAFLLDFPDDELRGDTLTVG
                     FWKRLRDEVKFDSIDELIAAIADDVERTRSIVTLQG"
     gene            complement(2516..>2866)
                     /locus_tag="VF036_07625"
     CDS             complement(2516..>2866)
                     /locus_tag="VF036_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265135.1"
                     /translation="FRRDRRSNFPAPGEPEGQSWVNDVHDMAAVLGGMAGTVGLGALA
                     PALARDPGLRDLAMPALSAAGASAGISAWFLRDVVRPGNGLLQRVNVTVPLVFMVRLG
                     LRMLSKRSVANPEG"
BASE COUNT          405 a         1001 c         1027 g          433 t
ORIGIN      
        1 gacgtgcgac tgcgcctcgt tcgaccggtt gggggtcctc ggcgaggccg cttcgggagc
       61 cggcgagctg atctggatcg atcaccatcg ctcgaacgac gggatcggca gcgtccgcct
      121 cgtggatccc gacgcgtcgt ccacgtgcga gatggtcttc cgcctgatcg agacgatggg
      181 cggcgacttc gatcgcgacg tcgccgtctg tctctacgcg ggcctggtca cggacaccgg
      241 tcggttccag taccaggcga ccaccccgga gacgctccgg atcgccgcgc ggctgcgcga
      301 ggaggacttc gaccacgcag gactcgtccg cgcgatgtac gaggacaacg acccgggctt
      361 cctccgcctg ctcgggacgg cgctcggacg gatgaccgag gtgcccgagg cgagcgtggt
      421 ctggacctac ctcacgcagg ccgatctcgc cgacgccggc gtcgcggcgg gggagaccga
      481 cgacgtgatc gacgtgctcc gcacggcgcg cgacgtggac gtcgccgcgg tcctgaagca
      541 gcagcgcgac gggcggttca aggtgagcgt gcgctcccgg ggcgggcacg acctctccgc
      601 cgtcgcgtcg gcgttcgggg gcggtggtca tcgcctcgcg gcgggctaca cgtcggagca
      661 cggacccgag gggacgatcg agcggctcgt ggccgccttg cgcccagcgc ccgttccctc
      721 gtgaccgccc agggccccga aggtgtgctc ctcctcgaca agccggcggg gatcacctcg
      781 cacgacgtcg tcgacagcgt gcgccggacc ctcgccacaa ggaaggtcgg gcacgccggg
      841 acgctcgatc cgatggcgac cgggctcctg gtgatcgggg tcggtcgcgc gacccggctg
      901 ctccgcttcc tcggtgatct cctgaagacc tacgagggga cgctccggct cggggtcgag
      961 acggacacgc tggatgccga cggcgcggtc gtgcgcgagt cgccggtcga tctcaccgag
     1021 ggccggctcg ccggagcgat gcaggccatg gtcggcgact cgctccaggc gcctcccgcg
     1081 tacagcgcgg tcaaggtggg cggccggaag ctctacgagg cagcacgtgc gggggagcgg
     1141 ctcgaggcga agtcgcggcc catccgcgtg gaagcgttcg aactgctctc gttcgatccc
     1201 ccggacgcca gcttccgcgt cgtctgctcc ggcggcacgt acgtccgggt cctcgcggcg
     1261 gatgtgggcg ccgcggtctc taccggagcg cacctgatga cactccgtcg cacggcgatc
     1321 ggcccgtacc gggtcgagga tgccgcgcgg ccggattccg tcggcgatcc gttcccgatc
     1381 gaggccgcgg tgcgccatct cccccggctc gatctcgacg ccgacgaggc cgaggccgct
     1441 tcgtacggga gaccgctcgg ccccgccgat ctcgaaggtc cgtacggtgt gttcgggccg
     1501 gacggccgtc tgatcggcgt ctacgaggac gacgggccca gggcgcgtcc gcacgtcatc
     1561 ctggctggct gaacggtcgg taccctgccg acgatggaca tcgtccgagg cctcgacgcc
     1621 cttccgttgc ccgacggtcc gtccgtcgta acgatcgggt tcttcgacgg cgttcacctc
     1681 ggtcaccagg aggtgttccg ccgcaccgtt gaggcggcga gggagcggga cgctcggagc
     1741 gtggccgtga cgttcgaccg gcatccgcgc gagacgctga cgccggggga ggagcccagg
     1801 ctgctcacga ccgtcgaacg gaaagccgcc ttgatcgacg cgtgcggcat cgatgtgctg
     1861 gtcgtgctcg cgttcacgca ggaattctcg ctcgtgcccg cggaaacctt cgttcgcgac
     1921 gtgctcgtcg gcaggttgca cgcggtgcac actcgggtcg gcgcgaactt cacgttcggg
     1981 caccgcgccg tgggcacggt cacgaccctc cgtgaggtgg gcgaaccgct gggtatgacc
     2041 gcggacgacg tcgatctgtt cgaactcgag ggtcgcgtcg tgtcctcctc gtcgatccgg
     2101 gacgcgctgg cgtcgggcga cctcgcgtgg ccgcgcaccg ccctcggcag gaggtacggc
     2161 gtcgatggag aggtcgtcgc gggcgccggc cgcgggaagg gcctcggcta cccgaccgcg
     2221 aacctccgca cgtggccgag gctcctcctt cctggccagg ggatctacgc cggcaccgca
     2281 gagctgttcg acggctcccg ctacccggcg gcgatcgacg tcgggacgaa cccgacgttc
     2341 ggaacggagc cgctccatgt cgaggcgttc ctgctggact tccccgatga cgagctccga
     2401 ggggacaccc tgaccgtggg gttctggaag cgcctacggg atgaggtgaa gttcgattcg
     2461 atcgatgagc tcatcgcagc gatcgccgac gacgtcgaac gcacccgatc gatcgtcacc
     2521 cttcagggtt agcgaccgag cgcttcgaca gcatccgcaa gccgagtcgg accatgaaga
     2581 cgagcgggac ggtgacgttc actctctgga ggagaccgtt ccccggcctc acgacgtcac
     2641 ggaggaacca cgccgagatg ccggcgctcg cgccggcggc gctgagcgcg ggcatcgcca
     2701 gatctcgaag tcccgggtcc ctggccagcg ccggcgcgag cgcgcccagc ccgaccgtcc
     2761 cggccatccc ccccagcacg gcggccatgt cgtgcacgtc gttcacccac gactgaccct
     2821 cgggctcgcc gggggccggg aagttggatc gccgatcgcg gcggaa
//
LOCUS       DASUVE010000352         2826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_738648, whole genome shotgun sequence.
ACCESSION   DASUVE010000352 DASUVE010000000
VERSION     DASUVE010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738648"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..1995
                     /locus_tag="VF036_07630"
     CDS             388..1995
                     /locus_tag="VF036_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265136.1"
                     /translation="MSAWSSAHLRGSADPVAASGDGDGLPPDLVTAASLDDDTVAPAD
                     GVLVLIADLCRSLEAERVRYCHWKSNESIARSASGENDLDLLVARADAERFERILRRL
                     GFKDTVQPAWKRLPGIYHSYGLDPTGLFVHIHAHYQLVIGDDMTKNIHLPIEAPYVAS
                     ARPEGMFPIPTTAFELLLFLIRMVVKHSTWDAFVSFQGSLSKSERRELEDLVGRTDLH
                     EVWELAERHLPFIPRELLERCLRCVQPGASRWLQIRTAARLQRALAGCSRRPPVLDTG
                     LRMWRRGRTVLRRKVLRRGPVRSRMSSGGALVAIVGGDGAGKTTVVDGLSDWLASENI
                     DSFTMHMGKPSWSALTTIVKGSLKLAAATKRTSTSSAKALRSSLSAGEGELTVRNRAR
                     LVWEVLTARDRFRTYRRARRLATNGAIVICDRYPLPEVSLMDGAVTGRLKDPSAWGRW
                     AAWLAGLERRYYERIRDPDTLIVLRVDPDTAVERRRGIEPESSVRPRSEEIWGIDWSG
                     TSAIVIDAASPKDDVLSEVKATIWSRL"
     gene            2069..>2826
                     /locus_tag="VF036_07635"
     CDS             2069..>2826
                     /locus_tag="VF036_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265137.1"
                     /translation="MVSRVVSDGSLTKKASLNAAASTAEQGARIVAGLIISPILLTQL
                     GDTVFGIWQVLQRLIGHASPASGRPGEALKWTIAHEQRSTDYDFKRRQVGNALAVWVL
                     FLPLLLAIGGVLAWLSPVWLDVPRDSYGTVRLAAGILVLDAVLFSLLYLPQSVLQGEN
                     LGYKRLGLSVLLVFVSKSLLVVAVYFGAGIGGLAVATAVATVLWGLTYLLIVRSRVEW
                     FGIARPSIAEVRHFVGLSWWFLLWNLVMTIMLAS"
BASE COUNT          435 a          920 c          981 g          490 t
ORIGIN      
        1 ggacgggagt acgagtaggc cgccgccaga gccacgacga cgatggcgac cgccagcacg
       61 agccacttgc gccggaggac cgtccccatg aactcccgaa ggtccagcga tccgtcgctg
      121 gatggcggcg ccccggagag aggttcctgg aacatcctgc tcatgcgatc cctgcctcct
      181 cgatccgatc ccccgtaggg atcggcgcgc ggcgtctacg gccggcgggt acgataccca
      241 cggtagaacc cggtgcgcgc ggccaccatc gtgcgaacgg cgtattgggg ctacggcgtg
      301 atgcgggtgc tgcgcgagcc gcgctcgcgt accgttgttc tgtcatcccg accgcggtgg
      361 agcaaccatg tccgacacag gcatcagatg agtgcgtgga gcagtgctca cctgcgcgga
      421 tccgccgatc cggttgccgc gagcggcgac ggagacgggc tcccacccga tctcgtgacg
      481 gccgcctcgc tcgacgacga caccgttgcg ccggcggatg gcgttcttgt gttgatcgcg
      541 gatctgtgtc gttccctcga ggcggagcgt gttcgctatt gtcattggaa gagcaacgag
      601 tcgatcgcgc gctccgcctc gggcgagaac gacctcgatc tgctcgtcgc gagggccgac
      661 gcggaacggt tcgaacggat cctcaggcgc ctggggttca aggacacggt gcaaccggca
      721 tggaagcggc tgcccggcat ctaccactcc tacgggctcg acccgacggg gctgttcgtc
      781 cacatccacg cgcactacca gctggtgatc ggcgacgaca tgaccaagaa catccacctg
      841 ccgatcgagg cgccgtacgt cgcgtcggct cgaccggagg ggatgttccc gatccccacc
      901 acggccttcg agcttctgct gttcctgatc cggatggtcg tgaagcactc cacctgggac
      961 gcgttcgtct cgttccaggg gtcgctgtcg aagagcgagc gccgggagct ggaggatctc
     1021 gtcgggagga cggatctgca cgaggtgtgg gagctcgcgg aacggcacct ccccttcatc
     1081 ccgcgcgagc tcctcgagcg atgcctgcgc tgcgtgcagc cgggcgcgtc gcgttggctc
     1141 cagatccgga ccgccgcccg cctgcaacgc gcgctcgccg ggtgcagccg gcggccgccg
     1201 gtcctcgata ccggtctgcg catgtggagg cgtggcagga cggtgctccg gcgcaaggtc
     1261 ctccgtcggg ggcccgtcag gagccggatg tcatccggcg gcgcgttggt cgccatcgtg
     1321 ggaggagacg gggcagggaa gacgaccgtg gtggacgggc tctcggactg gctcgcgtcc
     1381 gagaacatcg actctttcac gatgcacatg gggaaaccgt cgtggtccgc gctcacgacg
     1441 atcgtcaaag gctcgctgaa gctcgcggcg gcgacgaaac ggacctcgac gtcgtccgcg
     1501 aaggcgttgc gatcgtccct ttccgccggc gagggcgagt tgaccgtgcg gaaccgagcg
     1561 cgcctcgtct gggaggtcct cacggcccgc gatcgcttcc gcacctaccg gagggcgaga
     1621 cgtctcgcca cgaacggggc gatcgtgatc tgcgatcgct atccgctgcc ggaggtctcc
     1681 ctgatggacg gagccgtgac aggacggttg aaggatccgt cggcatgggg ccgatgggct
     1741 gcatggctgg ccggactcga gcgacgctac tacgagcgca tccgggatcc cgacacgttg
     1801 atcgtgctgc gggtcgatcc cgatacggcc gtagagcggc ggcgcgggat cgagccggag
     1861 tcatcggttc gtccgcgttc cgaggagatc tgggggatcg actggagcgg aacgtctgcg
     1921 atcgtgatcg acgcggcctc gccgaaggac gacgtcttgt ccgaggtcaa ggccaccatc
     1981 tggtccaggc tctgatgacg ccgagggacg gcggatgaag acggatgaca cgactccgag
     2041 gtccgcgccg agacgacggt tgggccggat ggtctctcgg gtggtctccg acggcagcct
     2101 gacgaagaag gcatccctca acgccgcagc gtcgacggcg gagcagggtg cgaggatcgt
     2161 cgccggtctg atcatcagcc cgatcctcct cacgcaactc ggggacacgg tattcggcat
     2221 ctggcaggtc ctgcagcgcc tcatcggcca cgcgtcaccg gcgagcggtc gccccggaga
     2281 ggcactgaag tggaccatcg cccacgaaca acgttcgacc gactacgact tcaagagacg
     2341 gcaggtcggc aacgccctcg cggtgtgggt gctcttcctt cccctgctcc tcgcgatcgg
     2401 cggcgtcttg gcctggctgt cgcccgtgtg gctcgatgtg ccgcgggact cctacggaac
     2461 cgtccgtctg gccgcgggca tcctggtgct cgacgcggtc ctcttcagct tgctctacct
     2521 tccgcagtcc gtcctccagg gggagaacct cgggtacaag cggttgggat tgtcggtcct
     2581 cctcgtcttc gtcagcaagt cgctcctcgt ggtcgccgtc tacttcggag cggggatcgg
     2641 cggcttggcg gtcgcgacgg cggtcgcgac cgtcttgtgg ggcctgacct atctgctcat
     2701 cgtccggagc cgcgtcgaat ggttcggcat cgcgcggccg agcatcgcgg aggtccgtca
     2761 tttcgtcggt ctgagctggt ggttcctcct gtggaacctc gtgatgacga tcatgctcgc
     2821 gagcga
//
LOCUS       DASUVE010000353         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_172355, whole genome shotgun sequence.
ACCESSION   DASUVE010000353 DASUVE010000000
VERSION     DASUVE010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172355"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1391
                     /locus_tag="VF036_07640"
     CDS             39..1391
                     /locus_tag="VF036_07640"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6265138.1"
                     /translation="MRLDRVAAAVLGLVLLTATCQDPPRARDTPDLLPPGRPERTDAS
                     AFDTRWPIKHVVFVIKENRTTDHLFGRFPGVNGVTEGYDGDRLRPLTPPLDRIPDDIP
                     HCYECAIESWNEGRMNNFARVEEGDLYAYTQMQGREDLPNYWRWAEEFALSDNFFASA
                     QGPSFPNHLFTIAAQSGGTHFNPNQEQSKVTIFTQETGLAKSWGCDALEEAFVEVVDS
                     EGQLEKVPPCFDFETEADLLDRAGIPWGYYAAWNDQRGYVWSAFSAIRRVRMNPEIWQ
                     RHIFPVDNFVADVEDGRLAPVTWVTPRYEVSEHPEWSFCHGENWTTEIVNSIMRSPFW
                     KDTAIFITWDDYGGFYDHVPPPQVDDFGFGFRVPLLTISPYAKPGYIDPQLGEFSSVL
                     RFIEDNWGLSQLTHRDRDAFNLSYLFDFDQEPREPFELPMREDCVGPIFAQPVTADGD
                     "
     gene            1477..1977
                     /locus_tag="VF036_07645"
     CDS             1477..1977
                     /locus_tag="VF036_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEX6265139.1"
                     /translation="MAEPKGELADLYVRHVPAANRLAFLLTGDRSHAEDLVHDAFVRC
                     VGRFGHLRAHEAFDAYLRRTIVNLHTSRLRRVRVEREFLSKEAGRVERTSMSSLPDVA
                     AREDMWRRLGTLPPRQRAVLVLRYYEDLSERETADALRCSVAAVKSLTARATSALREG
                     IRGEEG"
     gene            1974..>2186
                     /locus_tag="VF036_07650"
     CDS             1974..>2186
                     /locus_tag="VF036_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265140.1"
                     /translation="MTTDFENELRDLFREKAGEAPLATPAMPASAPRHVLRRGRLHQL
                     GTVLGCAAVVVALIVGSVAGRTRILGE"
BASE COUNT          361 a          732 c          736 g          357 t
ORIGIN      
        1 gggatcgctc aacgtgtccc gtccaatagc atcgccggat gcgactcgat cgcgtcgcgg
       61 ctgcggttct cggtctcgtg ctcctcacgg ccacctgtca ggacccgccc cgtgcgaggg
      121 acacgccgga tctcctcccg cccgggaggc cggagcgcac cgacgcctcc gccttcgata
      181 cgcgttggcc catcaagcac gttgtgttcg tgatcaagga gaaccggacg acggatcacc
      241 tgttcggccg gttcccgggc gtgaacggcg tcaccgaggg ctacgacggc gatcggttac
      301 gccccctcac gcccccgctc gatcggatcc ccgacgacat cccgcactgc tacgagtgcg
      361 cgatcgagtc gtggaacgag ggccggatga acaacttcgc gagggtcgag gagggggacc
      421 tctacgctta cacgcagatg cagggccgcg aggacctacc caactactgg cgctgggccg
      481 aggagttcgc gctctcggac aacttcttcg cgtcggcgca gggcccctcg ttcccgaacc
      541 acctcttcac gatcgccgcg caatctggtg gcacgcattt caaccccaac caggagcaat
      601 caaaggtcac gatcttcacg caggagacgg ggctcgcgaa atcatggggc tgcgacgcgc
      661 tcgaggaggc tttcgtcgag gtggtcgaca gcgaagggca gctcgagaaa gtgccgccgt
      721 gcttcgattt cgaaacggag gcggatctcc tggaccgcgc cgggatcccg tgggggtact
      781 acgcggcgtg gaacgaccag cgtgggtacg tgtggtccgc gttctccgcg atccgtcgcg
      841 tgcggatgaa cccggagatc tggcaacgcc acatcttccc ggtcgacaac ttcgtcgccg
      901 acgtcgagga cggccggctg gctcctgtca cgtgggtcac gccgcggtac gaggtgagcg
      961 agcaccccga gtggagtttc tgccacggcg agaactggac caccgagatc gtgaactcga
     1021 tcatgcggag cccgttctgg aaggacaccg cgatcttcat cacgtgggac gactacggcg
     1081 gattctacga ccacgtgccg ccgccgcagg tcgacgactt cgggttcggt ttccgtgtcc
     1141 cgctgctcac gatcagcccg tacgcgaagc ccggctacat cgacccgcag ctgggcgagt
     1201 tctcgagcgt cttgaggttc atcgaggaca attggggcct gtcgcagctc acgcatcgcg
     1261 accgagacgc gttcaatctg agctacctct tcgacttcga ccaggagccg cgggagccgt
     1321 tcgagcttcc gatgcgcgag gactgcgtgg gtccgatctt cgctcagcca gtcaccgcgg
     1381 acggagactg accgggctcc ggcgatacct tccaagcgcc tcgtgcgtct aggaggtatg
     1441 acaaggatcc tcctgggagc gcgggaagga acggcggtgg ctgagccgaa gggcgagctg
     1501 gcggacctgt acgtccgtca cgtgcccgcg gcgaaccggc tcgcgttcct cctgaccggc
     1561 gaccgctcgc acgcggagga cctcgtccac gacgcgttcg tgcggtgcgt cggtcggttc
     1621 ggccatctgc gcgcgcacga ggcgttcgat gcgtacctgc gacgcacgat cgtgaacctc
     1681 cacacgtcga ggctgcgccg cgtccgcgtc gaacgcgagt tcctctcgaa agaggccggc
     1741 cgcgtcgagc ggacgtcgat gtctagcctg ccggacgtcg ccgctcgcga ggacatgtgg
     1801 cgccgactgg gaacgctgcc accgcgccag cgagcggtgc tggtgctccg ctactacgag
     1861 gacctgtcgg agcgggagac agccgatgcg ctgaggtgct cggtcgccgc cgtgaagtcg
     1921 ttgaccgcgc gcgccacgtc ggcgctccgc gaagggatca ggggtgagga aggatgacca
     1981 cggacttcga gaacgagctt cgggacctgt tccgcgagaa ggccggcgag gcgccactcg
     2041 ccacgccagc gatgccggcg tccgcgccac ggcacgtgct acggcgcggc cgcctccacc
     2101 aactcgggac ggtcctcggc tgcgcagccg tcgtcgtcgc gctgatcgtc gggtcggtgg
     2161 ccggccgcac tcgcatcctc ggcgag
//
LOCUS       DASUVE010000354         3124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_363131, whole genome shotgun sequence.
ACCESSION   DASUVE010000354 DASUVE010000000
VERSION     DASUVE010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363131"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1668)
                     /gene="secA"
                     /locus_tag="VF036_07655"
     CDS             complement(<1..1668)
                     /gene="secA"
                     /locus_tag="VF036_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX6265141.1"
                     /translation="MIERFSNIGEGRRRKELEQLAKLVNTWELEIEELSDEDLAHKTV
                     EFRERLENGAPLDDLLPEAFAVTREAARRTIGQRHFDVQLMGGAALHHGGIAEMRTGE
                     GKTLVSTLPGYLNALEGEGVHLVTVNDYLAKRDSEWMGGIYRFLGLEVGLIQSQMSPD
                     QRRPAYAADITYGTNNEFGFDYLRDNMAMRLEHTVQRGHHFAIVDEVDSILIDEARTP
                     LIISGMVQDSAKWYQTFARIAPQLTRDEHYEVEEAKFQVAVTEPGVAKVEQLLNIDNL
                     YDHENTMLVHHLHNALRAKELYKRDDEYVVQSGEVKIVDEFTGRILEGRRYSEGLHQA
                     IEAKEGVRIKEENQTLATITIQNYFKMYDKLAGMTGTAKTQLTEFEETYEIGVTEIPT
                     NRPMVREDLQDLIFKNEEAKWNSLVDDIIERNETGQPILVGTVSIEKSEKLSGLLNRR
                     GVNHHVLNAKNHEKEAMIVAQAGRKGGVTVATNMAGRGVDILLGGNPEYLARQEMAAR
                     DFDNDRYLLFEMDEEERAAYEAEYEPIFAKFKAQTDAEHDEVVGLGGL"
     gene            complement(1765..>3124)
                     /locus_tag="VF036_07660"
     CDS             complement(1765..>3124)
                     /locus_tag="VF036_07660"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX6265142.1"
                     /translation="VAITAQAGLHRLYKESHQAVDLVQLFRPITKWGGMITVDEAVPE
                     MVRKAFKQARTERPGATFLILPEDVAELETTGTPLRVNVPVDPAPADAQIERAVEVLE
                     GATAPIVLAGPGVARDGAMDALVRVSERLHLPVATTFLGKGVFPDDHPNALGTIGFMV
                     RDYANFGFDDADVVVAVGYDLVEYAPDRWNPGRDKAIVHIHRTVAEVDEAYTLEVGVQ
                     GDIGAALDAISEAASGVFERDREPPAGRLVREELSSGADDDAFPVRPQRIVHDVREAL
                     GREDIVLCDTGAVKIWMARLFPTFAPNTCLISNGLATMAFALPGAFAAKLVHPERKVL
                     AAMGDGAFLMSAAEIETAVREKVHFVILVWVDGGYGLIGWKQDIHFGRRAAVSFGNPD
                     FVRFAESFGAKGYAIGEASELLPTLRKALDDDAVSVIAVPVDYGENAKLVERLGGLTE
                     PL"
BASE COUNT          438 a         1108 c          996 g          582 t
ORIGIN      
        1 cagcccgccg agcccgacga cctcgtcgtg ctccgcgtcg gtctgcgcct tgaacttcgc
       61 gaagatcggc tcgtactccg cctcgtacgc ggctcgctcc tcctcgtcca tctcgaacag
      121 gaggtaccgg tcgttgtcga aatcgcgggc cgccatctcc tgacgggcga ggtactcggg
      181 gttgccgccg aggaggatgt cgacgccgcg accggccatg ttcgtggcga ccgtgacccc
      241 gcccttgcgc cccgcctggg cgacgatcat cgcttccttc tcgtggttct tggcgttcag
      301 gacgtgatgg ttcacaccgc gccggttcag cagccctgac agcttctcgg acttctcgat
      361 cgaaaccgta ccgacgagga tcggttgccc tgtctcgttc cgctcgatga tgtcgtcgac
      421 gagcgagttc cacttcgcct cctcgttctt gaagatcagg tcctgcaggt cctcgcgaac
      481 catgggccgg ttcgtcggga tctcggtgac accgatctcg tacgtctcct cgaactcggt
      541 gagctgggtc ttcgccgtac ccgtcatgcc cgcgagcttg tcgtacatct tgaagtagtt
      601 ctggatcgtg atcgtggcga gcgtctggtt ctcctccttg atccgcacgc cctccttcgc
      661 ctcgatcgcc tggtggagcc cctccgagta ccggcgtccc tcgaggatcc gaccggtgaa
      721 ctcgtcgacg atcttgacct cgccgctctg gacgacgtac tcgtcgtcgc gcttgtagag
      781 ctccttcgcg cgcagggcgt tgtggaggtg gtggaccagc atcgtgttct catggtcgta
      841 gaggttgtcg atgttcagca gttgctccac cttggccact ccgggctccg tcacggcgac
      901 ctggaacttc gcctcctcga cctcgtagtg ctcgtcacgg gtgagctgcg gcgcgatccg
      961 cgcgaacgtc tggtaccact tcgcgctgtc ctggaccatg ccgctgatga tcagcggcgt
     1021 ccgcgcttcg tcgatcagga tcgagtcgac ctcgtccacg atcgcgaagt ggtgtccgcg
     1081 ctggaccgtg tgctcgagac gcatcgccat gttgtcgcgc aggtagtcga acccgaactc
     1141 gttgttcgtc ccgtacgtga tgtcggcggc atacgcgggc cgccgctggt cggggctcat
     1201 ctgcgactgg atcagcccga cctcgagacc gaggaaccgg tagatgccgc ccatccactc
     1261 cgagtcgcgc ttggccaggt aatcgttgac cgtgacgagg tggacaccct cgccctcgag
     1321 cgcgttcagg tagccgggca gcgtcgagac gagggtcttc ccctcgcccg tgcgcatctc
     1381 ggcgatccca ccgtggtgga gcgcggcccc gcccatcagc tggacgtcga agtgccgctg
     1441 gccgatcgtc cttcgcgcgg cctcgcgggt gacggcgaac gcctcgggga ggaggtcgtc
     1501 cagcggcgcg ccgttctcga ggcgctcccg gaactcgacg gtcttgtgcg cgaggtcctc
     1561 gtccgagagc tcctcgatct cgagctccca cgtgttcacg agcttcgcga gctgctcgag
     1621 ctccttccgt cggcggcctt cgccgatgtt gctgaagcgc tcgatcaggg gcacgacggg
     1681 tcctcttccg tggtcggggt ccttcgattc taccggcgct cccgtgtcag accgagatca
     1741 cgcggtccga cgggaagccg gtcgtcacag gggctccgtg agccccccga gccgctccac
     1801 gagcttggcg ttctccccgt agtccaccgg aaccgcgatc accgacacgg cgtcgtcgtc
     1861 gagtgccttc cggagcgtgg ggagcagctc tgatgcctcg cctatggcgt agcccttcgc
     1921 cccgaagctc tccgcgaacc gcacgaagtc cgggttgccg aacgagacgg ccgcccgccg
     1981 gccgaaatgg atgtcctgct tccacccgat caggccgtag cccccgtcca cccagaccag
     2041 gatcacgaag tgcaccttct cccgcaccgc ggtctcgatc tccgccgcgc tcatcaggaa
     2101 ggcgccgtcg cccatcgccg cgagcacctt gcgctcggga tgcacgagct tggccgcgaa
     2161 cgcacccgga agcgcgaacg ccatcgtggc caggccgttc gagatcagac acgtgttcgg
     2221 tgcgaacgtc gggaacagcc gcgccatcca gatcttcacc gcccccgtat cgcagaggac
     2281 gatgtcctcg cggcccaggg cctcgcggac gtcgtgcacg atgcgttgcg gacgcacggg
     2341 gaacgcgtcg tcgtcggcgc cggaactgag ctcctctcgg accagccgtc cggcgggagg
     2401 ctctcggtcg cgttcgaaga cgccggaggc cgcctccgag atcgcgtcca gggccgcgcc
     2461 gatgtccccc tggacgccga cctcgagcgt gtacgcctcg tccacctcgg cgaccgtccg
     2521 atggatgtgc acgatcgcct tgtcccggcc ggggttccaa cgatccgggg cgtactcgac
     2581 caggtcgtag cccaccgcca ccacgacgtc ggcgtcgtcg aaaccgaagt tcgcgtagtc
     2641 gcggaccatg aacccgatcg tcccgagcgc gttggggtgg tcgtccggga agaccccttt
     2701 gccgaggaac gtcgtcgcca ccggcaggtg cagccgttcc gagactcgca cgagcgcgtc
     2761 catcgcgccg tctcgggcga ccccgggccc ggccaggacg atgggggccg tcgctccctc
     2821 gagcacctcc accgctcgct cgatctgtgc gtccgccggc gcggggtcga ccgggacgtt
     2881 cacgcggaga ggcgtgcccg tggtctcgag ctcggccacg tcctccggca ggatcaggaa
     2941 cgtggcgccc ggccgctccg tccgtgcctg cttgaacgcc ttgcggacca tctcgggcac
     3001 ggcctcgtcc accgtgatca tcccgcccca cttcgtgatg ggccggaaga gctggacgag
     3061 atccacggcc tggtgggatt ccttgtacag gcggtgcagg ccggcctgcg ccgtgatcgc
     3121 gacc
//
LOCUS       DASUVE010000355         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_364010, whole genome shotgun sequence.
ACCESSION   DASUVE010000355 DASUVE010000000
VERSION     DASUVE010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364010"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1410)
                     /locus_tag="VF036_07665"
     CDS             complement(67..1410)
                     /locus_tag="VF036_07665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX6265143.1"
                     /translation="MRAFALEELGAAGSVVEVPVPEPAEGQVRIRVAAAGLNPFDNAV
                     IQGYLKDHMEHRFPLIPGADASGTVEALGNGVTDWAIGDEVFGSVGKGYQGEGTLAEL
                     ATMSTGTIARKPASVDHADAAAIPVAGVTALMMVEAASISEGDVVVAIGATGGVGSYL
                     VQLAAARGARVIAISSADNADTARKLGAADVVDYSAGDVVEAVRSLALSGVDVIAEMY
                     GGDDTARLAELVRSGGRVVSAVGGADEETLKARGIEAANVSGIVATDPLGTLVGMLER
                     GEIVSPEIRTFPLTSPPGRSSRWAPVTPAARSWYSRSARDPRRIYGRAVKFARIALVI
                     VLSFSAACASGSPRAAGTSTSGAASGSATPSNGTEPFEGDGAPLDPGRYATTRSPARR
                     SRSRSARVGSAVTPIRSSSTCNARRGVCCSDSRTRRSSSEPRASSRSMVEAPRTP"
     gene            complement(1512..>2537)
                     /locus_tag="VF036_07670"
     CDS             complement(1512..>2537)
                     /locus_tag="VF036_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6265144.1"
                     /translation="VAAGFDVLVDTNPTNPIEAEALASRDDVVAVAGLTRGVANFGAA
                     HLEGTPEWEISGFDLDLLRRGTPGLFRRGEAYASDAEVYLAVLDDPSLAIVPENFLVA
                     GVDVAVLDVGDTFTVIEPGSGRPHDLTIAALGETDWLENGALVSRELTTELFGEQDIV
                     TRYYLGVADGADPDAVASSVDADFLAQGADADTFSALINAGASKLTGFLALLRGFLGF
                     GLLVGIAGLGVVMVRAVRERRQEIGMLRAMGFRAGLIRTAMLSEAGLIAVQGTLIGAV
                     LGLITTRQLLMASDSFGDVPIPFIVPWVGLAVIVALPLVASLAVTAWPATRAARIRPA
                     VALRIAD"
BASE COUNT          417 a          918 c          833 g          369 t
ORIGIN      
        1 cgcggccgtc gatcgtcgcg gaccggaccg atccgccgac gaacggtggg ttcgccgcga
       61 tcgtgctcat ggcgtcctcg gcgcttcgac catcgatctc gacgacgccc tcggctccga
      121 cgacgaacgt cgggtccgcg aatccgagca gcacaccccc cgcctcgcgt tgcacgtcga
      181 agaactccgg atgggtgtga ccgccgatcc aacccgggcc gaccgcgaac gtgatcggcg
      241 ggccggtgaa cgagtcgtag cgtaacggcc gggatcgagc ggggcgccgt cgccctcgaa
      301 cggctcggtc ccgttcgaag gagtcgccga gccggacgcc gctcctgagg ttgacgttcc
      361 tgctgctctg ggggatccag aagcacaggc cgccgagaac gagagcacga tgaccagcgc
      421 gatacgagcg aacttcacgg ctcgaccata gatccgacgc gggtctcgcg cgctacggga
      481 gtaccacgat cttgccgcgg gtgtgaccgg agcccacctg ctcgaacgcc ctggaggact
      541 cgtcagcggg aacgtccgga tctcgggact cacgatctcc ccacgctcga gcatgccgac
      601 gagcgttccg agcgggtccg tcgccacgat cccactcacg ttcgcggcct cgatcccgcg
      661 cgccttcagc gtctcctcgt cggcgccgcc gaccgcggag acgacgcgtc caccggagcg
      721 aacgagctcc gcgagccggg ccgtgtcgtc gccgccgtac atctcggcga tcacgtcgac
      781 cccgcttagg gcgagcgagc gaaccgcttc aacgacgtcg ccggccgagt agtcgaccac
      841 atcggcggct cctagcttgc gggcggtgtc cgcgttgtcg gcgctggaga tggcgatgac
      901 ccgagcgccg cgcgcggccg cgagctgcac gaggtagctc ccgacgccgc cggtcgcccc
      961 gatcgcgacc accacatcgc cctcagagat ggaagcggcc tcgaccatca tgagggcggt
     1021 cacgccggca acggggatcg cggctgcgtc cgcgtggtcg acggacgccg gcttccgggc
     1081 gatcgtcccg gtggacatgg tggccagctc cgcgagcgtg ccctcgccct ggtacccctt
     1141 gcccaccgat ccgaacacct cgtccccgat cgcccaatcg gtaacgccgt tgccgagcgc
     1201 ttccaccgtc ccggatgcgt ccgcaccggg gatcagcggg aaccggtgct ccatgtgatc
     1261 cttgaggtac ccctggatca ccgcgttgtc gaagggattg aggccggccg cggcgacgcg
     1321 gatacggacc tgtccctcag cgggctccgg aacagggacc tcgacgacgg aaccggctgc
     1381 acccagctcc tccagggcga acgcgcgcat cgcttcctcc ttctcggcgg acagggctga
     1441 caaccctcga ccccatcacc gcattccgtc gcgtgacgat cagcgacgcg tgaccacggc
     1501 gagcgcccga ttcagtcggc gatccgcaac gcaacggcgg gccggatccg ggctgctcgg
     1561 gttgcgggcc atgcggtcac cgccagcgac gccacgagtg ggagggccac gatgacggcg
     1621 agaccgaccc acggcacgat gaacgggatc ggtacgtcgc cgaacgaatc cgatgccatg
     1681 agcagctgac gtgtcgtgat cagccccagc acggcaccga tcagcgtgcc ctgcaccgcg
     1741 atgagtccgg cctcggacaa catcgctgtg cggatgagtc cggcacggaa gcccatcgca
     1801 cggagcatgc cgatctcctg acgccgctca cggaccgccc ggaccatcac gacaccgaga
     1861 ccggcgatcc cgacgaggag gccgaacccg aggaagcctc gcaggagcgc gaggaagccc
     1921 gtgagcttgc tcgctcccgc gttgatgagc gcgctgaacg tgtcggcgtc ggctccctgc
     1981 gcgaggaagt ccgcatccac cgaagacgcg acggcatctg gatccgcccc gtccgccacc
     2041 cccaggtagt agcgagtgac gatgtcttgt tcgccgaaga gctctgttgt gagctcgcgg
     2101 ctcacgaggg caccgttctc cagccagtcc gtctcgccca gggccgcgat cgtgaggtcg
     2161 tggggccgcc cgctgcccgg ttcgatcacc gtgaaggtgt caccgacgtc cagcaccgca
     2221 acatccactc cggcgaccag gaagttctcc ggcacgatgg ccagggatgg atcgtcgagc
     2281 accgccaggt acacttcggc gtcgctcgcg taggcctcgc cccggcggaa caatccaggt
     2341 gtcccgcgtc tcagcaggtc gaggtcgaac ccgctgatct cccactcggg cgtgccctcg
     2401 aggtgcgcgg ctccgaagtt cgccacgcct cgtgtgaggc cggcgaccgc gaccacgtcg
     2461 tcgcggctcg ccagtgcttc cgcctcgatg gggttcgtcg gattcgtgtc gaccaggacg
     2521 tcgaagcctg cagcgac
//
LOCUS       DASUVE010000356         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1117130, whole genome shotgun sequence.
ACCESSION   DASUVE010000356 DASUVE010000000
VERSION     DASUVE010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117130"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VF036_07675"
     CDS             complement(<1..166)
                     /locus_tag="VF036_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX6265145.1"
                     /translation="MLKLVVPKGSLEEQTLRLFEAADLRVRRGSDRDYHGTVDDDRIE
                     RVSILRPQEIP"
     gene            265..1305
                     /locus_tag="VF036_07680"
     CDS             265..1305
                     /locus_tag="VF036_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX6265146.1"
                     /translation="MVESSTPRADGFTMPAEFDTHQATWMSWPTNRSLWDGHLDAARD
                     EWAATARAIAAFEPVVMVCNAGEASDVLRRCGEGVEPLELPIDDSWLRDNGPIFVRDE
                     RGELALVQFGFNSWGEKYLPYDKDARLPELLASHFGVRRYRAPFVLEGGSYFVDGEGT
                     LLTTEQCLMHPNRNPSMTREEIEEGLRSFLGVDTVIWLPHGLVEDRDTDGHIDGLAQL
                     VRPGVVMLAVASGDDDPNAPRFAEDRAILERARDATGRSFEVLDGPVNSWATVEGVGP
                     VVIPYMNHYLANGAVIVPIAGGPEDEPALELLGKAYPDREIVAVPGGMISHGGGGPHC
                     ITQQVPAGTFVS"
     gene            complement(1298..1624)
                     /gene="hisI"
                     /locus_tag="VF036_07685"
     CDS             complement(1298..1624)
                     /gene="hisI"
                     /locus_tag="VF036_07685"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX6265147.1"
                     /translation="MEQVQVDELLFDDAGLIPVIVQDADNGEVLMFAWSNREALERTI
                     AEGRMVYWSRSRRELWRKGDSSGHTQHWQELRADCDGDVLLARVHQEGAACHTGERSC
                     FHRWIS"
     gene            complement(1625..>2138)
                     /locus_tag="VF036_07690"
     CDS             complement(1625..>2138)
                     /locus_tag="VF036_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX6265148.1"
                     /translation="VRTDDDVDALLRAGADKVSVNTAAVRDPSLLSRCADRFGTQCMV
                     VAIDAKRRGDGWDVFVDAGRTPTGRDAVEWAAEVTARHGAGEVLLTSMDRDGTGDGYD
                     LELLRAIADAVAVPVVASGGAGTREHFAEALTDGRADAVLAASRWHDGDLTIREVKEH
                     LASRGIPVRL"
BASE COUNT          319 a          757 c          737 g          325 t
ORIGIN      
        1 gtgggatctc ctgcggccgc aggatcgaga cccgttcgat ccgatcgtcg tccacggtgc
       61 cgtgatagtc gcggtcggag cctcggcgca cgcggaggtc ggcggcctcg aacagccgca
      121 gcgtctgctc ctcgagcgag cctttgggga cgaccagctt cagcacgtcg acctcacggt
      181 ccggatgcgc acggagtgta cgtggagcgg gaggggacgc cgggcggtgc ggggaccggt
      241 gcgaaccggc tactgttccg cgccatggtc gagagctcta cccctcgggc cgacgggttc
      301 acgatgccgg ccgagttcga cacacatcag gccacctgga tgtcgtggcc gaccaaccgg
      361 tcgctgtggg acggccacct cgacgccgcc cgggacgagt gggccgccac ggctcgcgcc
      421 atcgccgcct tcgagcccgt cgtgatggtc tgcaacgccg gggaagcgag cgacgtcctc
      481 cggcgctgcg gcgagggtgt ggaaccgctc gagctgccca tcgacgactc gtggctccgg
      541 gacaacggcc cgatcttcgt acgggacgaa cgaggcgagc tggcgctggt ccagttcgga
      601 ttcaattcgt ggggagagaa gtacctgccg tacgacaagg acgcacggct cccggagctg
      661 ctcgcctcgc acttcggcgt ccgtcggtac cgggcgccgt tcgtcctcga gggcggctcg
      721 tacttcgtcg acggcgaggg cacgctgctg accacggagc aatgcctcat gcatccgaac
      781 cggaacccgt cgatgacgcg tgaagagatc gaggaagggc tccggtcgtt cctcggcgtc
      841 gacaccgtga tctggctccc gcacgggctc gtcgaggacc gcgacacgga cgggcacatc
      901 gacgggctcg cgcagctggt caggcccgga gtggtgatgc tcgcggtcgc ctccggcgac
      961 gacgacccga acgccccccg gttcgcggag gatcgagcga tcctcgagcg tgcgagggac
     1021 gctacgggcc gctccttcga ggtgctcgac gggcccgtga attcgtgggc gaccgtcgaa
     1081 ggcgtcgggc cggtcgtgat cccgtacatg aaccactacc tcgcgaacgg cgccgtgatc
     1141 gtcccgatcg cgggcgggcc ggaggacgag ccggcgctcg agctgctcgg gaaggcctat
     1201 ccggatcgcg agatcgtcgc tgtgccgggt gggatgatct cccacggcgg cggcgggccg
     1261 cactgcatca cccaacaggt gcccgcgggg acgttcgtca gctgatccag cgatggaagc
     1321 acgaccgctc gcccgtgtgg caggcggcac cctcctggtg gacgcgggcg agcagcacgt
     1381 cgccgtcgca atcggcacgg agctcctgcc agtgttgggt gtgaccggag ctgtcgccct
     1441 tccgccagag ctctcggcgt gaccggctcc aatagaccat ccgcccctcc gcgatcgtcc
     1501 gctcgagtgc ttcgcggttc gaccacgcga acatcaacac ctcgccgttg tcggcgtcct
     1561 ggacgatcac ggggatcagc cccgcgtcat cgaagagcag ctcatcgacc tgaacctgct
     1621 ccatctagag cctcaccggg atcccgcgag atgcgaggtg ctccttgacc tcccggatcg
     1681 tgaggtcgcc gtcgtgccac cgtgacgcgg cgaggacggc gtcggcccgc ccatccgtga
     1741 gcgcctcggc gaagtgctcg cgggtcccgg caccgcccga ggccaccacc ggcaccgcaa
     1801 ccgcatcggc gatcgcgcgc agcagctcga ggtcgtagcc gtcgcccgtg ccatcgcggt
     1861 ccatcgacgt gagcaggacc tcgccggcgc cgtgtcgtgc ggtcacctcg gccgcccact
     1921 ccaccgcgtc ccgtccggtc ggcgtgcggc ccgcatcgac gaagacgtcc catccgtcac
     1981 ctcggcgctt cgcatctatc gcgacgacca tgcactgggt gccgaaccgg tcggcgcacc
     2041 gcgacaggag cgacggatcg cgcacggccg ccgtgttcac gcttaccttg tcggcccccg
     2101 ctcgcaggag cgcgtccacg tcgtcgtccg tgcggacc
//
LOCUS       DASUVE010000357         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1681689, whole genome shotgun sequence.
ACCESSION   DASUVE010000357 DASUVE010000000
VERSION     DASUVE010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681689"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VF036_07695"
     CDS             complement(<1..833)
                     /locus_tag="VF036_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265149.1"
                     /translation="MRSGEDAADEAPSLRLVSSQGAAHNLPTRLTTFVGRGREMAEIG
                     RLLESTRLLSLVGAGGGGKTRLSVEFAASAIGDHPDGVWLVELASLSEGSLVLPAVAG
                     VFELREQPGVTPLDSVVAHLADRRLLVLMDNCEHLIGACAEAAQGILASCPDVKILAT
                     SREPLRVPGEVVLRVPPLLLPDPAGPIDPASIGSFDSVRLFTERAGDAAPGFELTPDT
                     AQHVADLCAHLDGLPLAIELAASRVGSFSVSTIVERLADRFGLLVGGNRTALSRQQTL
                     HA"
     gene            complement(872..>2042)
                     /locus_tag="VF036_07700"
     CDS             complement(872..>2042)
                     /locus_tag="VF036_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6265150.1"
                     /translation="VRGGGHSVPGFGTVDGGIVIDLALMREVTVDPTAKTARAQGGAT
                     WNVFNDATHVHGLATTGGFVSTTGIGGLTLGGGIGYLARGVGLSCDNLVGAEVVTAEG
                     EIVTATEDENGDLFWALRGGGGNFGVVTELRYHLHPVSDVYGGPMFFELSDGPALLRF
                     FRDLMTDAPEELGMFPAFQIAPPLPFIPEDRHGDTFMAMVACWAGPPEVGEKVLQAVH
                     DVAPVVAEHVGVAPYPALNSAFDALYTAGLQHYWKTSFATELTDSAIDVHMEYGPKVP
                     AVNSTMHIYALNGAVQRVAPDATAFAYRDAMFATNIAGMWPDPADNEANTAWVRDYYD
                     ALAPHSEAGGYVNFMSGDDRDRIEASYRGNYERLVEIKRAYDPGNLFRVNQNIEP"
BASE COUNT          331 a          712 c          691 g          308 t
ORIGIN      
        1 gcatggagcg tctgctgcct cgacagggcg gtgcggttcc ctcccaccaa gagcccgaat
       61 cggtcggcca accgttccac gatcgtcgag acggagaacg atccgacccg tgaagcggcg
      121 agctcgatcg ccaatggcag tccgtcgagg tgcgcgcaca gatcggcgac gtgctgcgcg
      181 gtgtcgggcg tcagctcgaa ccccggagcg gcgtccccgg cacgctccgt gaatagccgg
      241 accgagtcga acgagccgat cgacgccggg tcgatcgggc cggcgggatc gggaagcagg
      301 agcggcggca cccggagcac cacctcgccc gggacgcgga gcggttcccg gctcgtcgcg
      361 aggatcttga cgtccgggca ggaggcgagg atcccctggg cagcttcggc acaggctccg
      421 atcagatgct cgcagttgtc catcagtacg agcagccgac ggtccgcgag gtgggccacc
      481 acggagtcga gcggggtgac accgggttgc tcccgaagct cgaacacgcc cgcgacggcg
      541 ggcaggacga gcgagccctc cgacagcgac gccagctcga cgagccacac tccatcggga
      601 tggtcgccga tcgccgacgc cgcgaactcg acggacagac gcgtcttccc gcctccgccc
      661 gcgcccacca gggaaaggag gcgggtcgac tcgagcagcc gcccgatctc ggccatctca
      721 cgtccgcgcc cgacgaaggt cgtcaggcgg gtgggaaggt tgtgcgcagc tccttgactc
      781 gagacgagtc ggagcgacgg tgcctcgtcg gccgcatcct caccggatcg catcccgcct
      841 cctcccgctg gggctccggt caggtccgcg gtcacggctc gatgttctgg ttcaccctga
      901 agaggttgcc gggatcgtat gcccgcttga tctcgacgag gcgttcgtag ttccctcgat
      961 agctcgcctc gatccgatcg cgatcgtcgc cggacatgaa gttgacgtac ccgcctgctt
     1021 ccgagtgcgg ggcgagggcg tcgtagtagt cgcgcaccca cgccgtgttg gcctcgttgt
     1081 cagcggggtc cggccacatc cccgcgatgt tggtggcgaa catggcgtcc cgataggcga
     1141 acgcggtcgc atcgggagcc acacgctgga ccgcgccgtt gagcgcgtag atgtgcatcg
     1201 tcgagttcac ggcggggacc ttcggcccgt actccatgtg gacgtcgatg gccgagtccg
     1261 tgagctccgt cgcgaagctc gtcttccaat agtgctgcaa gcccgccgtg tacagcgcat
     1321 cgaacgcact gttgagagcg ggatagggag cgacgcccac gtgctccgcc acgaccggcg
     1381 ccacgtcgtg cacagcctgg agaaccttct ccccgacctc cggcggcccg gcccagcacg
     1441 caaccatcgc catgaacgtg tccccgtgcc ggtcctccgg gatgaacggg aggggcggcg
     1501 cgatctggaa cgcggggaac atcccgagtt cctcgggagc atccgtcatc aagtcccgga
     1561 agaaacggag gagcgccggg ccgtcggaga gctcgaagaa catcgggccg ccgtacacgt
     1621 cgctgacggg atggaggtga tatcggagct ccgtcacgac gccgaagttc ccgccccctc
     1681 cgcgcagcgc ccagaagagg tcgccgttct cgtcctcggt cgcagtcacg atctcgccct
     1741 cggcggtcac gacctcggct ccgacgaggt tgtcgcacga gaggccgact cctcgggcca
     1801 gatacccgat gccacctccg agcgtgagac cgccgatgcc cgtggtcgac acgaagcccc
     1861 cggtggtggc cagtccgtgg acgtgcgtcg cgtcgttgaa gacgttccag gtcgcccctc
     1921 cctgggcgcg agccgtcttc gccgtcgggt cgacggtgac ctcgcgcatc agcgcgaggt
     1981 cgatcacgat cccgccgtcg accgtcccga atcccggcac gctgtggccg cctccgcgga
     2041 cc
//
LOCUS       DASUVE010000358         6157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1117813, whole genome shotgun sequence.
ACCESSION   DASUVE010000358 DASUVE010000000
VERSION     DASUVE010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117813"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VF036_07705"
     CDS             complement(<1..602)
                     /locus_tag="VF036_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX6265151.1"
                     /translation="MAVDAERFDPNDIAEALRKHVESWTPSVERGELGRVIEAGDGIA
                     RVSGLPGAMANELLEFPGAVLGLAFNLDVGEIGCIVLGDASHIQEGDPVKQTGQILSV
                     PVGDGFLGRVVNALGEPIDDKGPIQSQDRRILEVQAPNVVNRQPVKEPLYTGITAIDA
                     MTAIGRGQRELLIGDRQTGKTAVAVDAIIAQKELWATGDP"
     gene            complement(608..1150)
                     /gene="atpH"
                     /locus_tag="VF036_07710"
     CDS             complement(608..1150)
                     /gene="atpH"
                     /locus_tag="VF036_07710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX6265152.1"
                     /translation="MTVDDTVRGYAQALLSIADAEGALDRVQDELYAFAKAVERNEDL
                     REALTDAALPPERKKAVIDELLGERAHPTTATLVGFLVDAGQARRIGAIAEELAKEAA
                     HRSEKTLAEVRVAVPLSDAKKSLLADALERATGHPVELKVDVDPTVIGGVVARVGDEV
                     FDGSLASRLTEAKQYLTGSV"
     gene            complement(1151..1759)
                     /locus_tag="VF036_07715"
     CDS             complement(1151..1759)
                     /locus_tag="VF036_07715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX6265153.1"
                     /translation="MHALIGSILAQAEEHGEEVSEAKDLYPAVGELIVGLLAFAVLFF
                     FTWKWVLPKFKEVLEQRGEQIQGEMEKAEATRKEADKLQEDYRTQLAGARDEANGIIE
                     EARATADQLRRDLQAKAEEESQAIVGRAQEEIRAERDRVFQELRAQVGSIAVEIAGRV
                     VGQSLDEKTHQRLIDEFIDEVASGAVANGNGNAKAGDAGSGD"
     gene            complement(1776..2021)
                     /gene="atpE"
                     /locus_tag="VF036_07720"
     CDS             complement(1776..2021)
                     /gene="atpE"
                     /locus_tag="VF036_07720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003935756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX6265154.1"
                     /translation="MLGLLAQAASDFSGLETIGKGLIIGLAAIGPGIGLGILIGKSVE
                     AMARQPEYANQVRTTMFIGIGLIEALALFGIAFSFII"
     gene            complement(2054..2848)
                     /gene="atpB"
                     /locus_tag="VF036_07725"
     CDS             complement(2054..2848)
                     /gene="atpB"
                     /locus_tag="VF036_07725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX6265155.1"
                     /translation="MIPAVLAEEFHPPSTKDFVFDCYFSVDLFGIEICFNFIIALVVA
                     SVLVYVLLFLLAFRKPRAVPGKLQALMEMGLEFVREQIALPMLGPAADRFLPLLVTFF
                     FFIFFMNMFEIAPWVNFSASSRVAFPLVLAMIAWVTYNAVGIAKHGFFGYLRFTCIIP
                     SAPAILRYTLLLVIEFVSNIVIRPITLTIRLAANFVAGHFLLALAFLGTEFFFLNGGL
                     SYLGLTVTLPISVALVAFEVFVAVLQAFIFAILTASYIGGVLAEEH"
     gene            complement(2845..3279)
                     /locus_tag="VF036_07730"
     CDS             complement(2845..3279)
                     /locus_tag="VF036_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265156.1"
                     /translation="MTTTVEPERELVRRLLPFAVPALGIAAGFGAVLGGSGAAWSAAI
                     AIVAVALNFVAYASSVAWAARISPMLVMAVGLGGYVVRVAAFTVALLLLNRLSWFSPL
                     AFVAAFAPATIALLVVEMRLLGGRMQADLWYFPEKTWPERTT"
     gene            complement(3276..3494)
                     /locus_tag="VF036_07735"
     CDS             complement(3276..3494)
                     /locus_tag="VF036_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX6265157.1"
                     /translation="MSGSRKSDAWSGMGIGWAITSTLIGGMATVGGLGYLLDRFLETG
                     SGFTALGIVLGAASAIYIVYLRYGKGDE"
     gene            complement(3648..4871)
                     /locus_tag="VF036_07740"
     CDS             complement(3648..4871)
                     /locus_tag="VF036_07740"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX6265158.1"
                     /translation="MVAYLIVFLVAAGTTFALTPLVRRLAIRFGAIDTPSERKLHTVP
                     TPTMGGLAMWAGFLVAIGVSRLLPFFEPMNATAVEPLAAVVTCTMMVGLGVIDDRRGT
                     SALAKLTAQIFIAGILILLGVQLAFFVLPGEGLAVLGTDEAVPLTILWVLAAANAINL
                     VDGLDGLAAGMVAIAAATFFIYMVRGADLSESASVAALLSAITVGVCLGFLPWNFHPA
                     KIFMGDTGSMLLGMLVAVATISGVGLNPFPPERGDVAVIALPLLVPLLVLAIPLLDVV
                     LAIVRRTRKGLGIAHADKEHIFHRLMDIGHGHRQTVLLMYLWSALVCGSALAIGVIDG
                     LLVAGVIVAGAVTLFLVTALPRLLERRRNGDANGGGEEAGSEDPAEPAGATMPASGLT
                     TKPAPGPAPVDPSAP"
     gene            complement(4960..>6157)
                     /gene="glyA"
                     /locus_tag="VF036_07745"
     CDS             complement(4960..>6157)
                     /gene="glyA"
                     /locus_tag="VF036_07745"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018639304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX6265159.1"
                     /translation="NELQRERTNLRLIASENYASPAVLAALGSTMTDKYAEGYPGRRY
                     YGGCEFVDVTEQLAIDRAKELFGAEHANVQPHAGAQANLAVFGAFLEPNDPDSKVLGM
                     ELAHGGHLTHGSPVNVSGMWFNFVGYRVDEDTEQLDMDEVRDLAKEHRPKIILAGYTA
                     YPRAIDFAAFRTIADEVGALLWVDASHFIGLVAGGAYPSPVPHADVVTFTTHKALRGP
                     RGAMIVCKEEHAKAIDKAVFPMMQGGPLEHVIAAKAVALKEAMQPAFRDYAQRVVRDA
                     RALAAGLADEGLRVISGGTDSHLILADVRPVGTDGMTAEAVCDEVGIALNKNQIPYDP
                     NPPSSPSGIRVGTPGPATLGMDEAEMGEVASIIGDVLKHPDDGSVKEKSGQRVRDLME
                     RFPLYP"
BASE COUNT         1023 a         2160 c         2027 g          947 t
ORIGIN      
        1 ttgggatccc cggtcgccca gagctccttc tgcgcgatga tcgcgtcgac ggcgaccgcg
       61 gtcttgcccg tctgccggtc cccgatcagc agctcacgct gcccgcgccc gatcgccgtc
      121 atcgcgtcga tcgccgtgat cccggtgtag aggggctcct tcacgggctg ccggttcacg
      181 acgttgggag cctggacctc gaggatccgc cggtcttgcg actggatggg tcccttgtcg
      241 tcgatcggct cgccgagcgc gttcacgacc cgcccgagga acccgtcgcc gaccggcacg
      301 gataggatct ggcccgtctg cttgaccgga tcgccttcct ggatgtgcga ggcgtcgccc
      361 aggacgatgc acccgatctc tccgacgtcg aggttgaacg ccagcccgag gacggccccg
      421 gggaactcca gcagctcgtt ggccatcgca ccgggaagac ccgacacgcg ggcgatgccg
      481 tcgccagcct cgatcacgcg gccgagctcg cctcgctcga ccgagggtgt ccagctctcg
      541 acgtgcttgc ggagggcctc cgcgatgtcg ttcgggtcga acctctctgc atccacagcc
      601 atcccgatca cacgctccct gtgaggtact gcttcgcctc ggtcagccgg ctcgcgagcg
      661 agccgtcgaa cacttcgtcg cccacgcggg cgaccacacc gccgatcacg gtggggtcga
      721 cgtcgacctt caactccacg ggatgtcctg tcgcgcgctc gagcgcgtcg gccagcaacg
      781 acttcttcgc atcgctcaat gggacggcga ctcgcacctc ggcgagcgtc ttctccgacc
      841 ggtgggcggc ctccttcgcc agctcctcgg cgatcgcccc gatccggcgc gcctgtcccg
      901 cgtccaccag gaaccccacg agcgtcgcgg tcgtgggatg cgcgcgctcg cccaggagct
      961 cgtcgatcac ggccttcttg cgctcgggtg ggagcgcggc gtccgtgagg gcctcgcgaa
     1021 ggtcctcgtt gcgctcgacg gctttcgcga acgcgtacag ctcgtcctgg acgcgatcga
     1081 gcgcaccctc cgcatctgcg atggagagga gcgcttgggc gtacccgcga accgtatcgt
     1141 cgaccgtcac tcagtcacca ctcccggcgt ctccggcttt ggcgttgccg ttgccgttcg
     1201 cgaccgcacc ggaggcgacc tcgtcgatga actcgtcgat caggcgctga tgcgtcttct
     1261 cgtcgaggga ctggccgacg acccgcccgg cgatctccac cgcgatcgat ccgacctggg
     1321 cccggagctc ctggaacacc cgatcgcgct cggcccggat ctcctcctgg gcgcggccga
     1381 cgatcgcctg ggattcctcc tcggctttcg cctgcagatc gcggcgcagc tggtccgccg
     1441 tcgcccgagc ttcctcgatg atgccgttcg cttcgtctcg ggcgccggcg agctgcgtcc
     1501 ggtagtcctc ctggagcttg tcggcctcct tgcgggtggc ctccgccttc tccatctcgc
     1561 cctggatctg ctcccctcgc tgctcgagga cctccttgaa cttcgggagc acccacttcc
     1621 acgtgaagaa gaacaggacg gcgaaggcga gcagcccgac gatgagctcg ccgaccgcgg
     1681 gatacagatc cttggcctcg gagacctcct ctccgtgctc ctcggcctgc gcgaggatcg
     1741 acccgatcag tgcgtgcacg agcggtctcc cctcctcaga tgatgaacga gaacgcgatg
     1801 ccgaacagcg cgagcgcctc gatcagcccg atgccgatga acatcgtggt ccggacctgg
     1861 ttcgcgtact ccggctgtcg agccatcgcc tcgaccgact tcccgatcaa gatcccgagc
     1921 ccgatcccgg gcccgatcgc cgcgagcccg atgatcagcc ctttgccgat cgtctccagc
     1981 cccgagaagt cacttgcggc ttgtgcgagc aaccccaaca tccattcctc cttggtttcc
     2041 ttcggcttgg tctctaatgc tcttccgcca ggaccccgcc gatgtacgac gcggtgagga
     2101 tggcgaagat gaatgcctgc aagaccgcca cgaacacttc gaacgcgacg agcgccacgg
     2161 agatcgggag ggtcaccgtg aggcccaggt agctcagccc tccgttcaag aagaagaact
     2221 cggtcccgag gaacgcgagc gcgagcagga agtgcccggc gacgaagttc gccgcgagac
     2281 ggatcgtcag cgtgatcggg cggatcacga tgttcgagac gaattcgatc accaggagca
     2341 gtgtgtagcg caggatcgcc ggtgcgctcg ggatgatgca cgtgaacctc aggtacccga
     2401 agaacccgtg tttcgcgatc cccaccgcgt tgtacgtgac ccaggcgatc atcgccagga
     2461 cgagcgggaa cgccacgcgg ctgctcgccg agaagttcac ccacggggcg atctcgaaca
     2521 tgttcatgaa gaagatgaag aagaagaacg tcaccagcag cgggaggaac cggtccgccg
     2581 ccggcccgag catcgggagc gcgatctgct cccgcacgaa ctcgaggccc atctccatca
     2641 gggcttgcag cttgcccgga acggcacgcg gcttgcggaa cgcgagcagg aacaggagga
     2701 cgtagaccag caccgaggcc acgacgaggg cgatgatgaa gttgaagcag atctcgatgc
     2761 cgaagagatc gacgctgaag tagcagtcga agacgaagtc cttcgtcgag ggcggatgga
     2821 actcttcggc gaggacagcc gggatcacgt ggttcgctcc ggccaggtct tctccgggaa
     2881 gtaccagagg tcggcctgca tccggccgcc gagcagccgc atctccacca cgaggagcgc
     2941 gatcgtggcc ggtgcgaacg cggccacgaa cgcgagcgga gagaaccagg agagtcggtt
     3001 caggagcagg agggctacgg tgaaggcggc aacccgcacc acgtagcctc cgaggcccac
     3061 ggccatgacg agcatcgggg agatgcgcgc cgcccacgcg accgagctcg cgtaggccac
     3121 gaagttgagg gccacggcga cgatcgcgat cgccgcggac catgcggctc ccgatccccc
     3181 gagcacggca ccgaagccgg ccgcgatccc gagggctggg acggcgaacg gcaagagccg
     3241 gcgcaccagc tcgcgctccg gctcaaccgt ggtcgtcact cgtctccctt gccgtaccgc
     3301 agatacacga tgtagatcgc gctcgccgcc ccgaggacga tgccgagcgc ggtgaacccg
     3361 gaacccgtct cgaggaaccg gtcgagcaga tatcccaacc ctccgaccgt ggccatccct
     3421 ccgatgaggg tggaggtgat cgcccagccg atccccatgc cggaccacgc atcgctcttg
     3481 cgggatccgc tcatcggagg cggacactct aaacggcccc ttcacatgcc gtcaaaccgc
     3541 ggaatcccgc gtttccgctg gtcagaggcc gtttccggga cctatgtccg cgagcttgtg
     3601 aaaggattca catggacccg ccacgcccat ccgaccgatc ggcgccctca aggggcgctc
     3661 gggtcgacgg gagccgggcc gggtgccggc ttcgtggtca ggcccgacgc gggcatggtg
     3721 gctccggccg gctcggcggg gtcctcggac cctgcctcct cgccgccgcc gttcgcatcc
     3781 ccgttgcgcc gacgctcgag gagacgtggc agcgccgtca cgaggaagag cgtgaccgcg
     3841 cccgcgacga tcacgccggc gacgagcagg ccgtcgatca ccccgatcgc gagcgcggag
     3901 ccacagacca gcgcggacca gaggtacatc agcagcaccg tctgccggtg tccgtgaccg
     3961 atgtccatga gccgatggaa gatgtgctcc ttgtccgcgt gggcgatccc gagccccttc
     4021 cgcgtgcgcc gcacgatcgc gaggaccacg tcgagcaacg ggatcgcgag gaccagcaga
     4081 ggcacgagca gcggcaacgc gatcacggcg acgtctccgc gctccggcgg gaagggattc
     4141 aggccgaccc ccgagatcgt ggcgaccgcc acgagcatcc cgaggagcat cgaccccgtg
     4201 tcgcccatga agatcttcgc gggatggaag ttccagggga ggaagcccag gcagacgccg
     4261 accgtgatcg cggacaggag cgcggcgacc gacgccgact cggagaggtc cgctccccgg
     4321 accatgtaga tgaagaacgt ggcggcggcg atcgccacca tgccggccgc gagcccatcc
     4381 agcccatcca cgaggttgat cgcgttcgct gccgccagca cccagaggat cgtgagcggg
     4441 acggcctcgt cggtccccag caccgcgagc ccctcgcccg ggaggacgaa gaacgcgagc
     4501 tggactccga gcaggatcag gatgccggcg atgaagatct gggccgtgag cttcgcgagc
     4561 gcggacgtgc ctcgccggtc gtcgatcacg ccgagaccga ccatcatcgt gcacgtcacg
     4621 accgccgcga gaggctcgac ggcggtcgcg ttcatcggct cgaagaacgg gaggagccgg
     4681 gagaccccga tcgccacgag gaagcccgcc cacatcgcga gcccccccat cgtcggcgtg
     4741 gggaccgtgt ggagcttgcg ttccgacggc gtgtcgatcg cgccgaagcg gatggcgagg
     4801 cgacgcacca ggggcgtcag cgcgaacgtg gtccccgctg cgacaaggaa cacgatcaga
     4861 tacgcgacca cggtggcctc agtgtgcggg ggtcctcgcc acccggcaac cggcggacct
     4921 gctcacagcc agcacggcca gatcgtcacg gccgcgtcgt cacgggtaga gcgggaagcg
     4981 ttccatcagg tcccggacgc gctgcccgga cttctccttc acggacccgt cgtccgggtg
     5041 cttcaggacg tcgccgatga tcgacgccac ctcgcccatc tctgcctcgt ccatcccgag
     5101 cgtcgcaggg cccggtgtgc cgacgcggat cccggagggc gacgagggcg ggttggggtc
     5161 gtacgggatc tggttcttgt tcagcgcgat cccgacctcg tcgcagacgg cctcggccgt
     5221 catgccgtcg gtgcccaccg ggcggacgtc cgcgaggatc agatgcgagt cggtccctcc
     5281 ggagatcacc cggagcccct cgtcggccag accggcggcg agtgcccgcg catcgcggac
     5341 cacgcgctgc gcgtagtcgc ggaacgccgg ctgcatcgct tccttgagcg cgacggcttt
     5401 cgcggcgatc acgtgctcga gcgggccgcc ctgcatcatc gggaacacgg ccttgtcgat
     5461 cgctttcgcg tgctcctcct tgcagacgat catcgccccg cggggccctc ggagcgcctt
     5521 gtgtgtggtg aacgtcacga cgtcggcatg cggcacgggc gagggatagg cgccgcccgc
     5581 gaccaggccg atgaagtggg aggcgtcgac ccacaggagc gcgcccacct catcggcgat
     5641 cgtccggaac gcggcgaagt cgatcgccct cgggtaggcc gtgtagcccg cgaggatgat
     5701 cttcggacga tgctccttcg cgagatcccg cacctcgtcc atgtcgagct gctcggtgtc
     5761 ctcgtcgacg cggtacccga cgaagttgaa ccacatcccg gagacgttca cgggcgagcc
     5821 gtgcgtgagg tgaccgccgt gggcgagctc catcccgagg accttcgagt cggggtcgtt
     5881 cggctcgagg aacgcaccga agacggcgag gttggcctgg gcgcccgcgt gcggctggac
     5941 gttcgcgtgc tccgcgccga agagctcctt cgcccggtcg atcgccagct gctcggtgac
     6001 gtcgacgaac tcgcatccgc cgtagtagcg tcgacccgga tagccttcgg cgtacttgtc
     6061 cgtcatcgtc gacccgagcg ccgcaagcac cgcgggactc gcgtagttct cggaggcgat
     6121 cagccgcagg ttcgtccgct cccgctgcag ctcgttc
//
LOCUS       DASUVE010000359         7307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1118263, whole genome shotgun sequence.
ACCESSION   DASUVE010000359 DASUVE010000000
VERSION     DASUVE010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118263"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1914)
                     /locus_tag="VF036_07750"
     CDS             complement(<1..1914)
                     /locus_tag="VF036_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6265160.1"
                     /translation="MRRRAWALYICGGVLVTAAYLFVPGLRLGPLFNVIAISSPIAIV
                     IAVRVWKPQTRLPWYLFALGQAFFVAGDVITYNYDRFFGVELPFPSVGDVFYLSVYPC
                     LILGILWLIRLRTPGGDRDSVIDSLIVAIGIGTVSWVFLLSPLARDSTSSLIQKLTAM
                     GYPVMDLILFTVVVRLAIGGGRRAPAFYLMAGATLALFLTDFMYSYISVSGAVYDNES
                     GILETGWATFYVLWGAASLQGSMRALSERTPDVEPRLTRGRLWLLALASLLAPSVMMI
                     QHIRNEAGDLNVAGDLPVLIGASAALFLLVVVRIAGLVRKQEQSTVRERALRGAGTAL
                     VTATNRDGIYAATLQAARSIAGETAVIRVLVGEEGEDYTVVAAAGGREDVVGVTLSLS
                     ILPQWKRDRLKTHRSIEVVVEESEIAGPLGFPPESAFVLSGPLFMKDELRGLLVVGSS
                     SALPRANRETLEALTSQVALALDSAFLTEDLFRQQTEARFRSLVQNSTDVVMVVDADS
                     TVRYVSPSVEGVFGYDPEELENTQLTLLIHPEDRTKVLQFLTLSGRDGVTSTGLIESR
                     MRHRDDFWLHIETLRTDLMHDPNVKGIVLNSRDVSERKAFEEQLSHQAFHDSITGLAN
                     RALFRDRVEHALER"
     gene            complement(1998..3332)
                     /locus_tag="VF036_07755"
     CDS             complement(1998..3332)
                     /locus_tag="VF036_07755"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6265161.1"
                     /translation="MNGLVERVRILRPLRHRDFRLLWVGMTASLIGDGIFIVSLAWQV
                     YQLSNAPTALSIVGVAMTVPQIVFLLLGGVVSDRFDRRRVMIAADALRALSLIALGLL
                     SIAPEGTLQIWHVMIVAAVFGAGTAFFGPAFDAIVPQLVPSDDLNQANSLDQFVRPAA
                     ARMLGPAVGGWLIAAWGPGTAFLIDACTFVVSIACVALMRARAVDKSEEHRSAFHDIR
                     EGFRFVRSRVWLWGTFLAATLAYLIFWGPSEVLLPFIVKNHMHGSASDLGFILALGGV
                     GAMFAAIVMGNREMPRKHITFMFGVWTLSTVAIAGYGLASFPWQLMVASFVFNALEGA
                     GTIVWATTKGRLVPARLLGRVSSFDWFISTGLVPVSFALTGPVAAIFGPRTTLVGAGI
                     LGGVVTLGFLFLPGMRDVERSGDLARASAEDPDRLLPPIDEPLLPSRAPAPL"
     gene            complement(3336..4031)
                     /locus_tag="VF036_07760"
     CDS             complement(3336..4031)
                     /locus_tag="VF036_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6265162.1"
                     /translation="MTKGPVAEGLGRVEELSDAGHYQAALDELDRQLAEHPNEPAVHT
                     ARGWALENLGPGGLAGARDAYARALELDPTALWAKEGLSNVFRRMGDNEEANRLAKQV
                     IEEGRPRAEKEIDLLELIGWCQLRLGLYPEAVATLRRALELDGRWTAVRFDLALALMC
                     EGDRGRAFLEYQRGIHQAVNGGVERLRGVVRMGLDDLEECLAERPALAGTREAIAARD
                     LLRSTLRVASQTA"
     gene            complement(4028..5437)
                     /locus_tag="VF036_07765"
     CDS             complement(4028..5437)
                     /locus_tag="VF036_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEX6265163.1"
                     /translation="MSLAERASRVPVFARPRSDAPRRRATDTVVTGPIGPQPFRRVGD
                     GWLDRWMETVADLARLKDSHPMLDAVIDEQEGRRIRIGDRWLFDFASCNYLGFDLDRE
                     IIDAVPAYLERWGTHPSWSRLLGSPVLYEEIEEKVTDLLGGEDTLLFPTITHIHMSVI
                     PVLAGAGTVFLDGRAHKTIYDGAMIARGHGATVERFRHDDPAHLEELLRASAISPRVI
                     ALDGVNSMLGNAPDLREFARLAREYDAVLYVDDAHGFGVVGERAPDELTPWGTRGNGV
                     IRHQGESYENVIFIAGFSKAYSSLLAFLTLPTRLKDVLKVAAPPYLYSGPSPIASLAT
                     TLAGLDVNERRGDRNRLEMYLKTERVLDRLHELDIYTPNRSGYPLIEVPLADHEDIDA
                     VGRYLFDRGIYVTMAAYPLVPKDEVGFRIQITAANGDEQIEQLCEVLGEINERFELQH
                     DGHRELLRERAARVGGITG"
     gene            complement(5607..6578)
                     /locus_tag="VF036_07770"
     CDS             complement(5607..6578)
                     /locus_tag="VF036_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6265164.1"
                     /translation="MRARARYGCAGLVLVVLSLTMCAQIEDPGSREPSPARPPRIPDG
                     SITVAAAGDISCPADDSDFEGEDPTECQQHATAALLADADAVLVLGDLQYPDGTLRTF
                     EFGYDASWGRYADKTYPAAGNHEYHVDGATGYFDYWAMKDRPTGGEGSGYYSFDLGSW
                     HLVALNSECDAVPCEEGSPQNNFLESDLMRTSQPCLLAFWHHPLFNSGATHGDSAPSG
                     ARAFWDDLTVAGADIVVNGHEHNYQRYGKQTGAGRAASQGIRQFVVGTGGKALYPMLE
                     EKDPNFEAGRASDFGVLKLHLGEGAYSWEFVSIDGTMVDSGGPVPCN"
     gene            6668..>7307
                     /locus_tag="VF036_07775"
     CDS             6668..>7307
                     /locus_tag="VF036_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6265165.1"
                     /translation="MPTVGIHASHEQLSPARLLDVVRAAEAAGFRAAMCSDHLAPWSE
                     RQGESGHAWAWLGAAMQATSLPLGVVTAPGQRYHPAMIAQAIATLSDLFPGRFWAALG
                     SGEALNEHVTGDPWPRKEDRDARLLECVDVIRALLRGEEVTHDGLVRADRARVWSLPA
                     EPPALYGAAVSEETARTVGSWADGMITVYQPIDVLRRVIDAFREGGGDRAPVA"
BASE COUNT         1241 a         2481 c         2491 g         1094 t
ORIGIN      
        1 ccgctccaac gcgtgctcga cccggtcccg gaacagcgcg cggttggcga gcccggtgat
       61 cgagtcgtgg aatgcctgat gggagagctg ttcctcgaac gccttccgct cggacacgtc
      121 tctcgagttg aggacgatgc ccttcacgtt ggggtcgtgc atcaggtcgg tgcggagggt
      181 ttcgatgtgc agccagaagt cgtcgcggtg ccgcatcctc gactcgatga ggccggtcga
      241 ggtcactcca tcccgcccgc tcagcgtgag gaactggagc accttcgtcc tgtcctcggg
      301 gtggatcagc agggtgagct gcgtgttctc gagctcctca gggtcgtagc cgaagacgcc
      361 ctccacggag gggctcacgt agcgaaccgt cgagtcggca tcgacgacca tgacgacgtc
      421 cgtcgagttc tggaccagag agcggaatcg agcttcggtc tgctggcgga acaggtcctc
      481 cgtcaggaag gcgctgtcga gcgccagcgc cacctgggag gtcagcgctt cgagggtctc
      541 ccggttcgcc ctcgggagcg cggagctgct cccgacgacg aggagccctc gcagctcgtc
      601 tttcatgaag agcggaccgc tcaacacgaa cgccgactcc ggcgggaacc cgagcggtcc
      661 ggcgatctcg gattcctcca ccaccacctc gatggagcga tgcgtcttga gtcggtcgcg
      721 cttccactgc ggcaggatcg agagcgagag tgtcacaccg acgacgtcct ctcgtccgcc
      781 ggcggccgcc acgaccgtgt agtcctcgcc ttcctcgccc accaacacgc ggatcaccgc
      841 cgtttcaccg gcgatcgaac gtgctgcttg gagggtcgcg gcgtagatcc catcacggtt
      901 cgtcgcggtc acgagtgccg tccccgcgcc acggagcgcc cgctcacgga cggtggattg
      961 ctcctgcttc cggacgagac cggcgatcct cacgacgacc aggaggaaca gtgctgccga
     1021 cgcgccgatc aggaccggca ggtcacctgc cacgttcagg tcaccagcct cgttgcggat
     1081 gtgctggatc atcatcacgc tgggcgcgag gagcgaggcg agcgcgagca accagagacg
     1141 gcccctcgtc aacctcggct cgacgtcagg ggtgcgctcc gacagcgccc gcatggaccc
     1201 ttgcaacgaa gccgcgcccc acaggacata gaaggtcgcc caccccgttt caaggatgcc
     1261 gctctcgttg tcgtacacgg cacccgaaac cgagatgtag ctgtacatga agtccgtgag
     1321 gaacagcgcg agcgtcgccc ctgccatcag gtagaaggcg ggggctcgcc gcccgccacc
     1381 gatcgcgagg cggacgacca cggtgaacag gatcaggtcc atcaccggat accccatcgc
     1441 cgtcagcttc tggatcagac tggatgtcga gtcgcgcgcc agaggtgaga gcaggaacac
     1501 ccacgagacc gtgccgatcc cgatcgcgac gatcagggag tcgatcacgc tgtcgcggtc
     1561 gccaccgggg gtgcggaggc ggatcaacca gaggatgccc aggatcagac acggatagac
     1621 cgagaggtag aagacgtcac cgaccgacgg gaacgggagc tcgaccccga agaaccggtc
     1681 gtagttgtag gtgatgacgt caccggcgac gaagaaggcc tggccgaggg cgaacaagta
     1741 ccagggcagc cgcgtctgcg gcttccacac acggacggcg atcacgatcg cgatcggcga
     1801 ggagatcgcg atcacgttga acagcggccc gaggcgaaga cccggcacga acaagtaggc
     1861 ggccgtcaca aggacgccgc cgcagatata aagggcccag gctctccgcc tcacaggttc
     1921 gatatcggtt ctgggggaag tcgaccttag tcggcgtgtt gcctgttcag gccccttgaa
     1981 aggggcccgg atccggctca caagggagcc ggtgcgcggc tggggagcag tggctcatcg
     2041 atcgggggca ggagcctgtc cggatcctct gccgaggccc gggcgaggtc accgctccgt
     2101 tcgacgtccc gcatgcccgg gaggaacagg aaccccagcg tcacgacccc gccgaggatg
     2161 ccggcaccca ccagggtcgt ccggggaccg aagatggcgg cgaccggtcc cgtgagggcg
     2221 aaggacaccg gcacgaggcc cgtcgagatg aaccagtcga agctggacac ccgcccgagg
     2281 agccgcgcgg gcacgagccg gcccttggtg gtggcccaca cgatcgtccc ggcaccctcc
     2341 agggcattga agacgaagct cgccaccatc agctgccagg ggaacgacgc cagaccgtag
     2401 cctgcgatgg cgaccgtgga gagggtccag accccgaaca tgaacgtgat gtgcttgcgg
     2461 ggcatctccc ggttccccat gacgatcgcc gcgaacatcg ccccgacccc tccgagcgcg
     2521 aggatgaatc cgagatcgct cgccgagccg tgcatgtggt tcttcacgat gaacggcagc
     2581 aggacctccg acgggcccca gaagatcagg taggccaggg tcgctgcgag gaacgtgccc
     2641 cagagccaga ccctcgacct gacgaaccgg aacccttcgc ggatgtcgtg gaacgccgag
     2701 cgatgctcct ccgacttgtc gaccgcccgg gcccgcatca gggccacgca cgcgatcgag
     2761 acgacgaacg tgcaggcgtc gatcaggaac gcggtgcccg gaccccacgc tgcgatcagc
     2821 cacccgccga ccgcgggacc cagcatgcgg gccgcggcgg ggcgcacgaa ctggtccagc
     2881 gagttcgcct ggttcaggtc atcgctcgga acgagctgcg gaacgatcgc gtcgaacgcc
     2941 ggcccgaaga aggccgtccc tgccccgaac acggcggcca cgatcatcac gtgccagatc
     3001 tggagggtcc cctccggggc gatcgacagc aggccgagcg cgatcaggct gagcgcgcgg
     3061 agcgcatccg ccgcgatcat cacgcgacgc cggtcgaacc ggtcgctgac gacgcccccc
     3121 aacaacagga agacgatctg cggcacggtc atcgcaacgc cgacgatcga cagcgccgtg
     3181 ggagcgttcg acagctgata gacctgccac gccagcgaga cgatgaagat cccatcgccg
     3241 atcagcgatg ctgtcatccc tacccagagc aagcggaagt cgcggtgccg gagaggccgg
     3301 aggatccgga cgcgctccac caatccgttc atgggtcacg ccgtctggga ggcaactcgg
     3361 agggtggatc ggagcaggtc gcgggcggcg atcgcctccc gcgtccccgc gagggcggga
     3421 cgctccgcca ggcactcctc gagatcgtcg agacccatgc ggacgacccc ccggagccgc
     3481 tcgacgcctc cgttgaccgc ctgatggatc ccccgttggt attcgaggaa cgccctgccg
     3541 cgatcgcctt cgcacatgag cgcgagcgcg aggtcgaacc gaacggccgt ccaccggccg
     3601 tcgagctcga gagcccggcg gagggtcgcc accgcctccg ggtacagccc cagccggagc
     3661 tggcaccatc cgatcagctc gaggaggtcg atctccttct ccgcccgcgg tctcccctcc
     3721 tcgatgacct gcttcgcgag ccggttggcc tcctcgttgt cacccatccg ccggaacacg
     3781 ttggagagac cttccttcgc ccacagcgcg gtggggtcga gctccagcgc gcgcgcgtag
     3841 gcgtcgcgcg caccagcgag accgccgggg cccaggttct cgagcgccca gccccgcgcc
     3901 gtgtgcacgg cgggctcgtt cgggtgctcc gcgagctgcc ggtcgagctc atcgagcgcc
     3961 gcctggtagt ggccggcgtc ggagagctcc tccacgcggc ccaacccttc ggcgactgga
     4021 cccttggtca tcccgtgatc cctcctactc gggcggctcg ttcccggagg agctcgcgat
     4081 gtccgtcatg ttggagttcg aaacgttcgt tgatctcgcc caagacctcg cacagctgct
     4141 cgatctgctc gtcgccgttc gcggcggtga tctggatccg gaagccgacc tcgtccttcg
     4201 ggacgagggg gtacgcggcc atcgtcacgt agatcccgcg gtcgaagagg tagcgaccga
     4261 ccgcgtcgat gtcctcgtga tccgcgaggg gaacctcgat cagcgggtag ccggatcggt
     4321 tgggcgtgta gatgtcgagc tcgtgcagcc ggtcgaggac ccgttcggtc ttcaggtaca
     4381 tctcgagccg gttccgatcg ccgcgtcgct cgttcacgtc gagacccgcg agcgtcgtcg
     4441 cgagcgacgc gatcggcgac ggccccgagt acagataggg aggagcagcg accttgagca
     4501 cgtccttcag tcgcgtgggc agggtgagga aggcgagcag ggacgagtac gccttggaga
     4561 agccggcgat gaagatcacg ttctcgtagg actcgccctg gtggcggatc acgccgttcc
     4621 cgcgtgtgcc ccagggggtg agctcgtcgg gagcgcgctc gccgacgacg ccgaacccgt
     4681 gggcgtcgtc gacgtagagg accgcgtcgt actcgcgcgc cagtcgagcg aactcccgga
     4741 ggtcgggcgc gttaccgagc atcgagttga cgccgtcgag cgcgatcacg cgtggcgaga
     4801 tcgcactcgc ccgcaggagc tcttcgaggt gcgccggatc gtcgtggcgg aagcgttcga
     4861 cggtcgcgcc gtggccgcgg gcgatcatcg ccccgtcgta gatcgtcttg tgcgcccgtc
     4921 cgtcgaggaa gaccgtccct gcgcccgcga gcaccgggat caccgacatg tggatgtggg
     4981 tgatcgtcgg gaacagcagc gtgtcctcgc cgccgaggag gtcggtcacc ttctcctcga
     5041 tctcctcgta cagcaccggg cttcccagga gccgggacca ggaggggtgg gtgccccagc
     5101 gttcgaggta cgcgggcacc gcgtcgatga tctcgcgatc gaggtcgaag cccaggtagt
     5161 tgcacgaggc gaagtcgaac agccatcggt caccgatccg gatccgccgg ccttcctgct
     5221 cgtcgatcac ggcgtcgagc atcgggtggg agtccttcaa ccgggcgagg tccgcgacgg
     5281 tctccatcca tcgatcgagc cacccgtcgc ccacgcgccg gaagggctgc ggtccgatcg
     5341 gaccggtcac gaccgtgtcg gtcgcccgac ggcgcggggc atccgatctc ggtcgggcga
     5401 acacgggtac gcgcgaggcg cgctcggcga gcgacatgga atccccatcg gccgaatggg
     5461 cgaggggctt tacacctaca acggggtatc tcgtcccagt ttgagcacga tggcaactgg
     5521 cgcgggacca ccaacggggg gacgcgagtt ccgagctctg taccccgatc gggggacaga
     5581 acgcgcaggc tggagcggat gcgggctcag ttgcacggaa caggaccacc cgaatcgacc
     5641 atggtcccgt cgatggagac gaactcccat gagtaggcgc cctccccgag atggagtttc
     5701 agcacaccga agtcgctggc gcgaccggcc tcgaaattgg gatccttctc ctcgagcatc
     5761 ggatagaggg ccttcccacc ggtgccgacg acgaactggc ggatcccctg cgatgccgct
     5821 cgcccggctc cggtctgctt tccgtatcgc tggtagttgt gctcgtgacc gttcacgacg
     5881 atatcggccc ccgcgaccgt caggtcgtcc cagaaggcgc gcgctccgga cggcgccgag
     5941 tcgccgtgcg tcgcccccga gttgaacagc ggatggtgcc agaaggcgag gaggcacggc
     6001 tgcgacgtgc gcatcaggtc gctctcgagg aagttgttct gtggtgagcc ctcctcgcac
     6061 gggacggcat cgcattcgga gttcagagcg acgaggtgcc acgagccgag gtcgaagctg
     6121 tagtagcccg atccctcccc gccggtgggt cgatccttca tcgcccaata gtcgaagtac
     6181 ccggtcgcac cgtcgacgtg atactcgtgg ttgccggcgg cggggtacgt cttgtcggcg
     6241 taccgccccc agctcgcgtc gtagccgaac tcgaacgttc gcagcgttcc atccggatac
     6301 tggaggtcgc cgaggaccaa gacggcatcg gcgtctgcga gcaacgccgc ggtcgcgtgc
     6361 tgttgacact cagtggggtc ctccccctcg aagtcggagt cgtccgccgg gcagctgatg
     6421 tcgcccgcgg cggcgacggt gatcgacccg tccgggatac gcggaggccg ggccggcgac
     6481 ggctctcggg atccgggatc ctcgatctgc gcgcacatcg tgagggacag caccacgaga
     6541 acgagccctg cgcatccgta tcgcgctctc gccctcaccc tgcccgaggg tagcgtccac
     6601 aggcgtccgg gaccaacgat cgccggtccg gtgcgtggac cgtgcctcct gcgggtacgg
     6661 acccagggtg cccaccgtcg ggatccatgc atcgcacgag cagctctcgc ccgcccgcct
     6721 cctcgacgtg gtgcgagcag ctgaggcggc cgggttccgg gccgcgatgt gctccgatca
     6781 cctggcgccg tggagcgagc gtcaggggga atccggtcat gcctgggcct ggctcggcgc
     6841 ggcgatgcag gcgacgtcgc tcccgctcgg ggtggtgacc gctcccggac agcgctacca
     6901 ccccgcgatg atcgcccagg cgatcgccac gctcagcgac ctgttccccg gcaggttctg
     6961 ggccgcgctc ggctcgggcg aggccctgaa cgagcacgtg accggcgacc cgtggccccg
     7021 gaaagaagat cgggacgcac ggctcctcga gtgcgtcgac gtgatccggg ccctcctccg
     7081 cggggaagag gtgacccacg acggcctcgt gcgcgcggac cgcgcgaggg tgtggagcct
     7141 gcccgccgag ccgcccgccc tctacggcgc cgcggtgagc gaggagacgg cgaggaccgt
     7201 cggctcctgg gccgacggca tgatcaccgt gtaccagccg atcgacgtcc tccggcgggt
     7261 gatcgacgcc ttccgcgagg gaggcggcga tcgcgcgccg gtcgccg
//
LOCUS       DASUVE010000360         4371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_177153, whole genome shotgun sequence.
ACCESSION   DASUVE010000360 DASUVE010000000
VERSION     DASUVE010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177153"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..904
                     /locus_tag="VF036_07780"
     CDS             242..904
                     /locus_tag="VF036_07780"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX6265166.1"
                     /translation="MARLPVDSVSLLVGAIWGLAAIFWIVLRRWEGTCVTEARRKELE
                     SEAEAVSPAIRAWRWMTVTITGAIPLLFAVDGFVDSIEILYAPELSFFAGPDLALQVV
                     GIVLSAAGLAILIGVGRKLAVYVYRRAIDEREMMTTGAHRYVRHPFYIHFLVLPIGLC
                     LLTLNYLALLVLAAYTTLWQPRTVVGWMRSEEDDLRRRYGDEAEAYLSRTGRVFPRLR
                     RP"
     gene            complement(969..2942)
                     /locus_tag="VF036_07785"
     CDS             complement(969..2942)
                     /locus_tag="VF036_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEX6265167.1"
                     /translation="MKDSRSGRADAWAFGAAPSSRPRRRGRGTLASLLILFAFAGGQA
                     SLIAVADQPVIESETTDGATAEATTSDETLPPEEAATEEVATEEVAKEEVATEDDVSA
                     EEPSAEDEAAGTVAAEEPAEATGDGSSDESATGSATEAAAVDTPGPPSGDGVQPILKT
                     GNPDCLNVMTSAQFLFEHKQDPPVDATINLTNGALSGTLVVAVNEGAQTFRFTLTGDF
                     VAAGVIVKGGSNANLYDYRPDGNAADTFLHAPVNPSNGKFYGLSHISFCLMEDVPPPP
                     TPVIDVEKSCPGSVDAGAEIEYTITVENTGNEALVDVSVDDTLLGDITADFDPALSQG
                     LAVGATATATVTHTAPGAGSVTNTVTATGTGAVSDTDDSDTASCETQITPPGPAIQIV
                     KGGAPELVHRGDTITYQFEVTNIGDVALFDVDLTDARCDAGTIDPGDDVDGVLGVDEV
                     WHFTCTHLVTQDDPDPIPNTATVRGDTVEGEGGDEVTDQDTFQVDIIHPGISIVKTVS
                     DATVPEGTVVTYTYVVTNTGDTTLFGISVNDDVIGPIGTIASLAAGASATLTGTFIVG
                     DSPTINIGTASGDDILGRTVTAEDDAVVTPIAGSGGGDGPGCCIAGGGGGLGGTPFTG
                     SEVGLWSLLAAALTLIGGLTLWATRRRLDGPTV"
     gene            3042..3665
                     /locus_tag="VF036_07790"
     CDS             3042..3665
                     /locus_tag="VF036_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX6265168.1"
                     /translation="MLGVEGIAGYFTALGLSTAAGLNAYIPILSVGLLSRSTDLIDLP
                     APWDALGDPIVLGIVGVVGVADFIGDKIPIVDHVLHAIGLAIAPIAGGMLALASASAF
                     DLDPGLAAGMGIVAALATQAGRTAARPLSTATTGGGGNPAVSLAEDGVSGVLSVTAVF
                     WPVVTAVLAVIVLVAVFLLWRKWRQLRTRFRRRDVRRPDPRLVGGPR"
     gene            3677..>4371
                     /locus_tag="VF036_07795"
     CDS             3677..>4371
                     /locus_tag="VF036_07795"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX6265169.1"
                     /translation="MKAPMNAKEIARRRIGNSRLTGDRFAAPDDVVRWHLAMQSQEYG
                     PAKWSIGQRSSGLDDADVDAALTEGTIVRTHVLRPTWHFVARDDIRWLLALSGPRVQQ
                     GNAGRNRQLGLDARTLTRAEKVVASILEGGNRLTRDELATELDRRKLDRGGQRMPYLL
                     MHCELVGLIGSGGRKGKQHSFALLDERAPAQPRALDRDDALVELVRRYLGSHGPATVK
                     DMSWWTGLTMTDLR"
BASE COUNT          595 a         1463 c         1571 g          742 t
ORIGIN      
        1 agaggggcga catcgtcgtc acgcacagcc tgttgaagtt cgcacctgac gaccggggca
       61 ccgtcgcggt ggatctcttc cgtctccagg acggcaaggt cgtcgagcac tgggacgtgt
      121 tgcagccctt ccccgaggag agcgcgaacc cgcatccgat gttctagcgc cgtgggggtc
      181 tgaggggcgg cacagacctc gggtcgccac tcgaaccgat cccttccact atcgtcgccg
      241 cgtggcgagg ctgccggtcg acagcgtgtc gctgctcgtc ggagcgatct gggggctggc
      301 ggcgatcttc tggatcgtcc tccggcggtg ggagggaacg tgcgtcacgg aggctcgccg
      361 gaaggagctc gaaagcgagg ccgaggccgt gtcacccgcg atccgtgcgt ggcgatggat
      421 gaccgtcacg atcacgggcg cgatcccgct gctgttcgcg gtcgatgggt tcgtggactc
      481 gatcgagatc ctctacgcgc cggagctgtc cttcttcgcc ggacccgacc tcgcgttgca
      541 ggtcgtgggc atcgtcctct ccgcagcggg gctggcgatc ctgatcggcg tgggccggaa
      601 gctcgcggtc tacgtgtacc gccgggcgat cgacgagcgg gagatgatga cgaccggtgc
      661 ccaccgatac gtgcggcacc ccttctacat ccacttcctc gtgctgccga tcgggttgtg
      721 cctgctgacc ctgaactacc tggcgctcct cgtcctggcc gcgtacacga cgctctggca
      781 gccgaggacg gtcgtcgggt ggatgcgctc ggaggaggac gatctgcggc gccggtacgg
      841 cgacgaggct gaggcgtacc tcagccggac cgggcgcgtc ttccctcggc tccgacggcc
      901 gtgatgcggc ctcggagatg agcgcggccc tccgttcccg gagggccgcg ttcgtcctgc
      961 tctgcgcgct agacggtcgg accgtcgaga cgccggcggg tcgcccagag ggtgagcccg
     1021 ccgatcaagg tgagggcggc cgcgagcagc gaccacagcc cgacctccga tccggtgaag
     1081 ggggtcccac cgagaccacc gccgccacca gcgatgcagc agcccggccc atctccgccg
     1141 cccgagccgg cgatcggcgt gacgaccgcg tcgtcctcgg cggtgaccgt ccgaccgagg
     1201 atgtcgtccc ccgatgcggt cccgatgttg atggtcggcg agtccccgac gatgaacgtc
     1261 ccggtgagcg tcgcggacgc accggccgcg agactggcga tcgtgccgat gggcccgatg
     1321 acgtcatcgt tgacgctgat gccgaacagg gtggtatcgc cggtgttggt gacgacgtac
     1381 gtgtaggtca ccacggtgcc ctccgggacg gtggcgtccg acacggtctt cacgatcgag
     1441 atccccggat ggatgatgtc cacctggaac gtgtcctggt cggtgacctc gtccccgccc
     1501 tcaccctcca cggtgtcgcc tcgcacggtg gcggtgttcg ggatcgggtc gggatcgtcc
     1561 tgggtgacca ggtgcgtgca tgtgaagtgc cagacctcgt ccacccccag gaccccgtcg
     1621 acgtcgtcgc ctgggtcgat cgtcccggcg tcgcaccgcg cgtccgtcag gtccacgtcg
     1681 aagagcgcga cgtctccgat gttcgtgacc tcgaattggt aggtgatcgt gtcgccccgg
     1741 tgcaccagct ccggcgcgcc gcccttgacg atctggatcg ccggacccgg cggggtgatc
     1801 tgggtctcgc aggaggcggt gtccgagtcg tccgtgtcgg agaccgcgcc cgtgcccgtc
     1861 gcggtcacgg tattggtgac cgagccggcg ccgggcgccg tgtgggtgac ggtcgcggtc
     1921 gcggtcgcac cgaccgcgag cccctggctg agggcgggat cgaagtcggc cgtgatgtca
     1981 cccagcaggg tgtcgtcgac ggagacgtcg accagcgcct cgttgccggt gttctcgacc
     2041 gtgatcgtgt actcgatctc ggccccggcg tcgacggagc ccgggcagga cttctcgacg
     2101 tcgatcacgg gcgtcggcgg cggcgggacg tcctccatga gacagaagga gatgtgggac
     2161 aggccgtaga acttcccgtt cgacgggttc accggagcgt ggaggaaggt gtcggcggcg
     2221 ttcccgtccg gccggtaatc gtagagattg gcgttcgagc cgcccttcac gatcacgccg
     2281 gcggcgacga agtcacccgt gagcgtgaat cggaaggtct gcgccccctc gttgaccgcc
     2341 accacgagcg tgccgctcag ggcgccgttc gtgaggttga tcgtggcgtc cactggtggg
     2401 tcctgtttgt gctcgaacag gaattgggcc gaggtcatca cgttcaggca gtcgggattc
     2461 cccgtcttga ggatcggctg cacgccgtcg cccgaagggg gtcccggggt gtcgacggcg
     2521 gccgcctcgg tcgctgatcc ggtggccgac tcgtccgagg agccgtcgcc ggtggcctcc
     2581 gcgggttcct cagccgcgac ggttcccgcg gcctcatcct cggccgatgg ctcttccgcg
     2641 ctgacatcgt cttccgttgc aacctcttct ttcgcaacct cttccgtcgc gacctcttcc
     2701 gttgccgctt cctccggcgg gagggtctcg tccgaggttg tggcctcagc ggtcgcgccg
     2761 tcggtggtct cggactcgat gacaggctga tccgcgacgg cgatcagcga agcctgtccg
     2821 ccggcgaacg cgaacaggat gagcagtgac gcgagtgttc cccgtccccg acgacggggg
     2881 cggctggatg gtgcggcgcc gaacgcccag gcgtcggcgc gtccggatct actgtccttc
     2941 atccttccct cccaagatgt cgctctgtcg acctacgccg gatacccgga tggttgcggg
     3001 tgcaaccgta cgctgattgc gaccgacgtg ccgcggatat cgtgctcgga gtggaaggga
     3061 tcgcgggata tttcacggcc ctcgggctct cgacagcagc cgggctcaat gcctacatcc
     3121 cgatcctgag cgtgggtctg ctctcccgct cgaccgacct gatcgacctg ccggcgccgt
     3181 gggacgcgct cggcgatccg atcgtgctgg gcatcgtggg cgtcgtcggc gtggcggact
     3241 tcatcgggga caagatcccg atcgtggatc acgtgctcca cgcgatcggc ctggcgatcg
     3301 cgccgatcgc cggggggatg ctggcgctcg cgtcggcgag cgcgttcgat ctcgatccgg
     3361 ggctggcggc gggtatgggg atcgtcgcgg cgctcgccac gcaggcgggc cgcacggccg
     3421 ctcggcccct ctccacggcc acgacgggcg gaggcgggaa cccggcggtg agcctcgccg
     3481 aggacggcgt ctcgggggtc ctctcggtga cggcggtgtt ctggcccgtc gtcacggccg
     3541 tgctcgccgt gatcgtgctg gtggcggtct tcctgctgtg gcggaagtgg cggcagctcc
     3601 ggacacgctt ccggcgccgg gacgtgcggc gacctgatcc tcggctggtc ggaggccccc
     3661 ggtaggctgc gcgccgatga aggcgccgat gaacgcgaag gagatcgcac gccgtcggat
     3721 cggcaactcg cgcctcacgg gagatcggtt cgcggccccg gacgacgtcg ttcggtggca
     3781 cctcgcgatg cagtcgcagg aatacggccc ggcgaagtgg tcgatcgggc agcgctcttc
     3841 gggcctggac gacgcggacg tggacgccgc cctcaccgag ggcacgatcg tgcggacgca
     3901 cgtgctccgg cccacgtggc acttcgtcgc gcgcgacgac atccgctggc tcctcgcgct
     3961 cagcgggccg cgcgtgcagc aggggaacgc gggcaggaac cggcagctcg gactcgatgc
     4021 tcgcaccctg acgcgcgccg agaaggtggt cgcctcgatc ctcgagggtg ggaaccgcct
     4081 cacccgggac gagctcgcca ccgagctcga taggcggaag ctcgatcgcg gcggccagcg
     4141 gatgccctac ctcctgatgc attgcgagct cgtcgggctg atcggcagcg gtgggcggaa
     4201 gggcaagcag cattcgttcg cgctcctcga cgaacgggcg cccgcgcagc cgcgcgcact
     4261 cgatcgggac gacgcgctcg tcgagctggt ccgccgctac ctcgggagcc acggccccgc
     4321 gaccgtgaag gacatgagct ggtggacggg actcacgatg acggatctcc g
//
LOCUS       DASUVE010000361         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_745080, whole genome shotgun sequence.
ACCESSION   DASUVE010000361 DASUVE010000000
VERSION     DASUVE010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745080"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..275)
                     /locus_tag="VF036_07800"
     CDS             complement(66..275)
                     /locus_tag="VF036_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018218908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265170.1"
                     /translation="MDQGTIRDFDEVGRTGVLLTDDRAEIVIDADSLDASGIRTLRIG
                     QRVKFETEERDGATVARSLRIVTWD"
     gene            complement(287..931)
                     /gene="cofC"
                     /locus_tag="VF036_07805"
     CDS             complement(287..931)
                     /gene="cofC"
                     /locus_tag="VF036_07805"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX6265171.1"
                     /translation="MRAIALPVKSLGHAKSRLSSLLAPLERGALALAMFEDVLDATLA
                     VDGWETWVVSSDESVLEIALARGAHGISELRPTLRSATLQIEAEAAARSTEALAILLA
                     DTPLVTLEALRAVLHTLGPVVIAPSSDERGTNLLLRRPPGAIPSRFGPDSFRRHLQTA
                     AERGLPTAVVERDELSFDVDEPGDILTVLETRRPGRTLDVCRDMDLAGRLAART"
     gene            1027..1656
                     /locus_tag="VF036_07810"
     CDS             1027..1656
                     /locus_tag="VF036_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6265172.1"
                     /translation="MLPPLRFWFRLSYEHLDRIPRSGPAIVACNHISYLDPLTNGDAI
                     VRARRRPRFLAKEDLFRIPLVGRALRGAGQIPVSRGTRDRSSLDLAVAALDRGEVIVV
                     YPEGTVTGRVDGLPMDGKTGLVRVALATGVPIIPMASWGSGAVWQKSGRGSLKFGRPI
                     WTTVGEPISLPAEADGGQVELRALTGEVMDAITQLAVDLRDRYPRRWTK"
     gene            1667..>2430
                     /locus_tag="VF036_07815"
     CDS             1667..>2430
                     /locus_tag="VF036_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX6265173.1"
                     /translation="MSDPGKRRRFQTRTVHPHRPAMPEQPSSSVPIFQSSTFRFETNE
                     EFARAIRFDGPGYVYSRGYGNPTVDAFQSAMADLEDAESAIGFASGMAAISSLLLTVA
                     SAGSRIVAGTALYGGTVSMLQHVLPRFGIETTFVDPTDVDQVRDALPGADLFYCETIV
                     NPTTTVPDLAVLAELCRDAGVLSAVDNTFASPSLCTPISLGFDVVLHSATKYIGGHAD
                     LIGGVACTNEELFRRLRATVIDLGGSMQPLEAWLCLR"
BASE COUNT          364 a          847 c          840 g          379 t
ORIGIN      
        1 gttcacgccg ggcaaggagt tccgccacca ggtcctgggc aacaactccg gttagaacgg
       61 cgcggtcagt cccaggtcac gatgcgaagg gatcgagcga ccgtcgcccc gtctcgctcc
      121 tccgtctcga acttcacccg ctgaccgatc cggagcgtgc ggatccccga ggcatcgagt
      181 gaatctgcat cgatcacgat ctccgcgcgg tcgtcggtga gcagcacgcc ggtgcgcccc
      241 acttcgtcga agtccctgat cgtcccctga tccacgctcg ctcctgtcag gtccgtgccg
      301 ccagcctgcc ggcgagatcc atgtcccggc acacgtcgag cgtgcgcccc gggcgccggg
      361 tttcgagcac cgtgaggata tcgcccggct cgtccacgtc gaaggacagc tcgtcccgct
      421 cgacgacggc cgtcgggagg ccgcgttcgg cggccgtttg gaggtgtcgc cggaacgagt
      481 ccggaccgaa gcgactgggg atggcccccg gaggccggcg gagcaggagg ttcgtccccc
      541 gttcgtccga ggagggggcg atcaccacgg gaccgagcgt gtgcaacacc gctcgcagtg
      601 cctcgagtgt cacgagcggg gtgtcggcca ggaggatcgc gagcgcctcg gtggatcggg
      661 ccgcggcctc cgcctcgatc tggagcgtcg cggagcggag cgtcggtcgg agctccgaga
      721 tcccgtgcgc ccctcgagcc agggcgatct ccaggacgct ctcgtcggac gagacgaccc
      781 acgtctccca gccgtcgacc gccagggtgg cgtccaagac gtcctcgaac atcgccaggg
      841 cgagcgcgcc ccgctcgagg ggagcgagga gcgaggacag ccgggacttc gcgtggccga
      901 gggacttcac ggggagggcg atcgcgcgca cggctctcca gcctagcgag ccctcgcggt
      961 accctcacgg cgctcgagcg aggagggacg acaacggaac cctggtacca cgtcgccaag
     1021 gcggtggtgc tcccaccgct gcggttctgg ttccgcctgt cgtacgagca cctggatcgc
     1081 atccccagga gcggaccggc gatcgtcgcg tgcaatcaca tctcgtacct ggatcctctg
     1141 acgaacggtg acgcgatcgt gcgcgcccgc cgccgcccgc ggttcctggc gaaggaggac
     1201 ctcttcagga tcccattggt ggggcgcgcg ctccgcggag ccggccagat cccggtctcg
     1261 cgtggcacgc gcgaccgatc ctcgctcgac ctcgccgtcg cagcgctcga tcgtggggag
     1321 gtgatcgtcg tctaccccga gggcacggtc acggggcgtg tggacggatt gccgatggat
     1381 ggcaagaccg gcctcgtccg ggtggcgctc gcgaccggcg taccgatcat cccgatggcc
     1441 agctggggat cgggcgccgt ctggcagaag tcggggcggg gatccttgaa gttcggccga
     1501 ccgatctgga ccacggtcgg cgagccgatc tcgctccccg ccgaagcgga cggggggcag
     1561 gtggagctgc gtgcgctcac cggggaggtc atggacgcga tcacccagct cgccgtcgat
     1621 ctccgagacc gctacccacg gcgatggacg aagtaggctg cccgccatga gcgacccggg
     1681 gaaacggcgc cgattccaga cgcgcaccgt gcatccccat cggcccgcga tgcccgagca
     1741 gccgtcgagc agcgtgccga tcttccagtc ctcgacgttc cggttcgaga cgaacgagga
     1801 gttcgcgcgc gcgatccgat tcgacggtcc gggctacgtg tactcgcgcg ggtacgggaa
     1861 ccccaccgtc gacgcgttcc agtccgcgat ggcagatctc gaagacgcgg agtccgcgat
     1921 cggattcgcc tccgggatgg cggccatctc ttccctcttg ctgaccgtcg cgagcgcggg
     1981 ttcgcggatc gtggcgggca ccgcgctcta cggaggcacg gtgtcgatgt tgcagcacgt
     2041 ccttcctcgc ttcgggatcg agacgacgtt cgtggacccc acggacgtcg accaggtccg
     2101 tgacgctctc cccggcgccg acctcttcta ctgcgagacg atcgtgaatc ccacgaccac
     2161 ggtcccggat ctggcggtgc tcgccgagct gtgccgcgac gcgggggtcc tctccgccgt
     2221 cgacaacacg ttcgcgtcgc cctccctgtg cactccgatc agcctgggct tcgacgtggt
     2281 gctgcactcc gcgacgaagt acatcggcgg tcacgcggac ctcatcggcg gggtcgcgtg
     2341 caccaacgag gaactcttcc ggaggctgcg cgccaccgtc atcgacctgg gggggagcat
     2401 gcaaccgctg gaggcctggc tctgtctgcg
//
LOCUS       DASUVE010000362         3977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_931752, whole genome shotgun sequence.
ACCESSION   DASUVE010000362 DASUVE010000000
VERSION     DASUVE010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931752"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="VF036_07820"
     CDS             complement(<1..1337)
                     /locus_tag="VF036_07820"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX6265174.1"
                     /translation="MVDETRFIFVTGGVASGLGKGITTASLGRLFKARGQKVLVQKLD
                     PYINVDPGTMNPFEHGEVFVLDDGAETDLDLGHYERFLDQDLHRGSNFTTGAVYSSVL
                     AKERRGDYLGKTVQVIPHITDEIKERIRALAETEQADIQIVEIGGTVGDIESLPFLEA
                     VRQLRNEIGRDRCAFVHVSLMPYIGPSGEIKTKPTQHSVKELRSLGLQPDAIVCRSDR
                     PIGRNLKEKISLLCDVPISGVISAPDARSIYEVPLTLHQEGLDAELAKHLGIDGEPDL
                     AEWQDLVAKVRAATDPVRIAVVGKYVNLRDAYLSVMEALNHGGIHHGVDVRIDWIASD
                     DLGGDATEQALEGADGVLIPGGFGIRGVEGKVDAVRFARERGVPFLGICLGLQCAVIE
                     FARHVCGLADANSSEFAPGTPDPVIDLLPEQKDVTDLGATMRLGAQACYLEPDT"
     gene            complement(1384..2193)
                     /locus_tag="VF036_07825"
     CDS             complement(1384..2193)
                     /locus_tag="VF036_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265175.1"
                     /translation="MVAWFAGGLALAWIGWLGARRAGARWWKENRRGRVIPATLGWAV
                     AMGAAGLALVVVRQIDADGVRDSQAGELLGAGIVFLAGVVDDGFGGAERGLRGHLRAL
                     LAGRLTTGSVKLAAAVLAAVITVAWTPREHLWANLLALIAIAGCTNVWNGLDVAPGRA
                     LKGFLVVAVVLLVVDLRAFLLVCTGAACAVLAPDLRERGMLGDSGANLLGFLAGAEIV
                     RRLPEIWLLPAALVVIGLNVLAETVTFSKTVETVPPLRWFDRLGRLPDATS"
     gene            complement(2208..2900)
                     /locus_tag="VF036_07830"
     CDS             complement(2208..2900)
                     /locus_tag="VF036_07830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6265176.1"
                     /translation="MERQVVALVAAHEESERVASTVSALHTLVDEVIVVDDGSTDATS
                     SAALLAGATVLRASQRLGKGRAIEEALGRLPGSAVWLLADADLAESAALLGPLIGAVR
                     EGRADIAIAVFPKLAGGGFGIVKRFAARSIRALCGWETRAPLSGQRALTAYGLTAVRP
                     LAPGFGLEVGMTIDAVRSGLRVIEIPVEGLTHRPTGRGPRGFAHRARQGVDIVGAVVA
                     RAVPGLPRRRRS"
     gene            complement(2900..3838)
                     /locus_tag="VF036_07835"
     CDS             complement(2900..3838)
                     /locus_tag="VF036_07835"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HEX6265177.1"
                     /translation="MISWRYHVVSIVAVILAFGLGILAGTSVVGDRFAADLQLNYDEA
                     IRERDAALDMVALRQRFADGLGPTLRDGVLTGEEAVVVTVEGVDGPAQIAVDELTAAG
                     VDVLAMLTIDRRLTDVPEDDTVSVIEEILGVTGTDPDSLEARIADALAVRLAVGPDGD
                     TDLLGALLAAGLITADRDLDEDALPGIGGAGQLVVVAAGGRSPDRVSPQTLLVPFFER
                     LVQLGAPTSAVGPTDDPFGLVSAIREAPGIADCASVTVDDLDLEGIGGITMVMGIEQL
                     LADDDPAFRPGGDYGFGGDAMMVVPGGDEPPPSCRA"
BASE COUNT          656 a         1426 c         1363 g          532 t
ORIGIN      
        1 gtgtcgggct cgaggtagca ggcctgggcc cccagccgca tcgtggcgcc caggtcggtc
       61 acatccttct gctccgggag gaggtcgatc accgggtcgg gcgtgccggg tgcgaactcg
      121 gacgagttcg cgtccgcgag gccacagacg tgacgggcga actcgatcac cgcgcactgg
      181 agcccgaggc agatcccgag gaacgggacg ccgcgctccc gagcgaaccg gaccgcatcg
      241 accttgccct ccacgccgcg gatcccgaag ccgccgggga tgagaacgcc gtcggcgccc
      301 tcgagcgcct gctcggtggc atcacctccg aggtcgtcgg acgcgatcca gtcgatgcgc
      361 acgtccacgc cgtgatggat cccgccatgg ttcagggcct ccatcaccga gagataggca
      421 tcgcggaggt tcacgtactt gccgacgacg gcgatccgaa cggggtcggt cgccgcgcgg
      481 accttcgcga cgaggtcctg ccattcggcg aggtcgggtt cgccgtcgat cccgagatgc
      541 ttggcgagct ccgcgtccag gccctcctga tgcaacgtga gcgggacctc gtagatcgac
      601 ctcgcgtccg gcgcgctgat cactccgctg atcggcacgt cacacaggag cgagatcttc
      661 tccttcaggt tgcgcccgat cggtcgatcg ctccggcaga cgatcgcgtc cggctggaga
      721 ccgagggacc gcagctcctt cacggagtgc tgggtgggct tggtcttgat ctcccccgac
      781 ggtccgatgt acggcatcag cgagacgtgc acgaacgcgc accggtcgcg accgatctcg
      841 ttgcggagct ggcggaccgc ctcgaggaac gggagggatt cgatgtcacc gaccgtgccg
      901 ccgatctcca cgatctggat gtcggcctgc tcggtctccg cgagagcgcg gatgcgctcc
      961 ttgatctcgt cggtgatgtg cgggatcacc tggaccgtct tcccgaggta gtcgccgcgt
     1021 cgctccttgg ccaggacgct cgagtagacg gctccggtgg tgaagttcga cccgcgatgg
     1081 aggtcctgat cgaggaaccg ctcgtagtgg ccgaggtcga ggtcggtctc ggcaccgtcg
     1141 tcgagcacga acacctcgcc gtgctcgaac gggttcatcg tgcccggatc gacgttgatg
     1201 tacgggtcaa gcttctggac gagcaccttc tggccacggg ctttgaagag gcggccgagg
     1261 gaggcggtgg tgatgccctt gccgagtccg gaagcgactc cgcccgtgac gaagatgaac
     1321 ctcgtctcat cgaccacggg aagcgactct aacagaccgg accgacggga ccgcccgttc
     1381 cgctcacgaa gttgcgtccg gcagccgacc gaggcgatcg aaccatcgga gcggagggac
     1441 cgtttcgacc gtcttcgaga acgtgacggt ctccgcgagc acgttgagcc cgatgacgac
     1501 cagggcggcc ggcagcagcc agatctcggg gaggcgccgc acgatctccg ctccggcgag
     1561 gaagccgagg aggttcgcgc ccgagtcgcc gagcatcccg cgctcgcgca ggtcgggagc
     1621 caggaccgcg cacgcggcgc ccgtgcacac gaggaggaac gccctcagat cgacgacgag
     1681 gagcacgacc gcgacgacga ggaacccttt gagcgcccga ccgggggcca cgtcgaggcc
     1741 gttccagacg ttcgtgcagc cggcgatcgc gatcagcgcg aggaggttcg cccacaggtg
     1801 ctctcgcggg gtccacgcca cggtgatcac cgcggcgagg acggcggccg cgagcttcac
     1861 gctcccggtg gtgagccgac cggcgaggag ggcgcgcagg tgcccgcgga ggccacgctc
     1921 cgctcccccg aagccgtcgt ccaccacgcc ggccaggaac acgatccccg ccccgaggag
     1981 ctcgccggcc tgggaatctc gcaccccgtc ggcgtcgatc tgccggacga ccacgagcgc
     2041 gagccccgcc gcccccatcg cgaccgccca gcccagcgtg gcggggatca cgcggccccg
     2101 gcggttctcc ttccaccagc gagcgcccgc tctgcgggct cccagccagc cgatccaggc
     2161 gagcgccagg ccccccgcga accacgcgac caccgccgcc ggctcgatca ggacctccgg
     2221 cgccgaggca gtccgggcac ggctcgcgcg acgaccgctc cgacgatgtc gaccccttgg
     2281 cgcgcacgat gcgcgaaccc acgggggccg cgcccggtcg gacggtgggt caatccctcc
     2341 acggggatct cgatcacccg taggccggag cggaccgcgt cgatcgtcat ccccacctcg
     2401 aggccgaacc cgggagcgag cgggcggacg gcggtcaggc cgtaggcggt gagcgcgcgt
     2461 tgtccggaca gcggcgctcg cgtctcccac ccgcagaggg cgcggatcga acgggcggcg
     2521 aaccgcttca cgatcccgaa ccctcctccg gcgagcttcg ggaagaccgc gatcgcgata
     2581 tcggcccggc cctccctgac cgcgccgatc agcggaccga ggagcgctgc gctctcggcc
     2641 agatcggcgt cggcgagcag ccacacggcc gaccccggga gacgacccag cgcctcctcg
     2701 atcgcacggc ccttcccgag gcgttgggac gcgcgcagga ccgtcgcgcc ggcgaggagc
     2761 gcggccgagc tcgtcgcgtc cgtggagccg tcatccacca cgatcacttc gtcgaccaga
     2821 gtgtggagcg cggagacggt gctggcgacg cgctccgatt cctcgtgcgc ggccacgagc
     2881 gccacgacct gacgctccat caggctcgac aggatggcgg cggctcgtcg ccccccggaa
     2941 cgaccatcat cgcgtcgccc ccgaacccgt agtcgccgcc gggccggaac gcgggatcgt
     3001 cgtcggcgag gagctgctcg atccccatca ccatcgtgat gccgccgatc ccctcgagat
     3061 cgaggtcatc gaccgtgacc gaggcgcaat cggcgatgcc gggcgcctcc cggatcgccg
     3121 agacgagacc gaaggggtcg tcggtcgggc cgactgccga cgtcggggcg ccgagctgga
     3181 caaggcgttc gaagaatggg acgaggagcg tctggggaga gacgcgatcc ggcgagcggc
     3241 cgcccgcggc gaccaccacg agttgtccag caccgccgat gccaggaagg gcgtcctcgt
     3301 cgaggtctcg atcggccgtg atgagcccgg cggcgaggag ggcgccaagc aggtccgtgt
     3361 ccccgtccgg tcccacggcc agccgaacgg ccaacgcgtc cgcgatccgc gcctcgaggg
     3421 agtccgggtc cgtcccggtg acgccgagga tctcctcgat caccgacacc gtgtcgtcct
     3481 ccggcacatc ggtgagccgc cgatcgatcg tgagcatggc gaggacgtcg acacctgccg
     3541 ccgtgagctc gtcgacggcg atctgggccg ggccgtcgac cccctccacc gtgaccacca
     3601 ccgcttcctc gccggtgagc acaccgtcgc ggagggtcgg ccccaggcca tcggcgaatc
     3661 gctgccgaag cgcgaccatg tcgagagccg cgtcccgctc acggatcgcc tcgtcgtagt
     3721 tgagctgcaa gtcagcagcg aaccgatcgc cgacgaccga tgttcccgcc aggatcccga
     3781 gcccgaacgc aaggatcacg gccacgatcg agacgacgtg atagcgccag gagatcacag
     3841 gatcccctgc agcagatcca cgaagtcgtc gagcgtctga tccacgtaga gacggagacc
     3901 gcgtgacgcg aagagcatca gaccgaacgt gatgagcacg gccccgacca agatcaccac
     3961 gtcccggacc cgagcac
//
LOCUS       DASUVE010000363         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_931794, whole genome shotgun sequence.
ACCESSION   DASUVE010000363 DASUVE010000000
VERSION     DASUVE010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931794"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VF036_07840"
     CDS             complement(<1..610)
                     /locus_tag="VF036_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX6265178.1"
                     /translation="MACLELDDRILIIDVGLSFPSGDMPGIDLVLPDFDFLRTKADRI
                     QAVVLTHGHEDHIGALPYLLRDIDRRLEVYGTPFTLALLQGKLEEHEVEHLVDLMTVS
                     PGEQASTGPFTMRFLRVTHSIPDGMAVVVDTPFGSILHTGDFKIDQTPLDGRATDLHG
                     LAQEAGRGKGVHLLLSDSTNAEEPGYSVSERTVGPVLLDIVEN"
     gene            complement(663..1547)
                     /gene="dapA"
                     /locus_tag="VF036_07845"
     CDS             complement(663..1547)
                     /gene="dapA"
                     /locus_tag="VF036_07845"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX6265179.1"
                     /translation="MAGRFGSVVTAMVTPFRPDHGVDLDGAQELASWLTDNGSDSVFV
                     VGSTGESPTLSHNEKADLFRAVGEAIRGKGKMICGTGTYSTAETLELTQAAEDAGADG
                     IVVVTPYYNKPPQRGLVAHFEHIAEATELPIIVYNIPSRTGTRIEHETMLRIAEKPNI
                     VAVKDSTGDFKAISNLISEAPDGFEVYSGDDWAAFGYVCLGAVGVVSVASHLVGLQMK
                     QMIDLIETGDVPAARKLHEELSPLFNALFVTSNPIPVKAALDMLGRPAGPPRLPLVPA
                     TPDERERIRRALEDAGLL"
     gene            1628..>2005
                     /locus_tag="VF036_07850"
     CDS             1628..>2005
                     /locus_tag="VF036_07850"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HEX6265180.1"
                     /translation="MPRIYTKTGDDGTTGLLYGGRVPKDDAATEAYGTTDEAVAVLGL
                     ARASAEPVMAEELLALQRELFIVGADLATNPAERAKLQPGVSLVTSDMTERLERRIDE
                     LVAERALPDAFIVPGANEGSAALD"
BASE COUNT          323 a          666 c          705 g          311 t
ORIGIN      
        1 cgttctcgac gatgtcgagc aggaccggcc cgacggtgcg ctcgctgacg gagtacccag
       61 gctcctccgc gttcgtggag tcggagagca gcagatggac gcccttgccg cggcccgcct
      121 cctgggccag gccatggaga tcggtggccc tgccgtcgag cggggtctgg tcgatcttga
      181 agtcgcccgt gtggaggatc gagccgaacg gcgtgtcgac gaccacggcc atcccatcgg
      241 ggatcgagtg ggtgacgcgg aggaaccgca tcgtgaacgg gcccgtcgac gcctgctcgc
      301 ccggcgaaac ggtcatgaga tcgacgaggt gctcgacctc gtgctcctcg agtttgccct
      361 gcagcagcgc gagcgtgaac ggcgtgccgt agacctcgag acggcggtcg atgtcgcgga
      421 gcaggtacgg cagcgctccg atgtgatcct cgtgcccgtg ggtgaggacg accgcctgga
      481 tccgatcggc cttcgttcgc aggaagtcga agtccggcag gaccaggtcg atcccgggca
      541 tgtcacccga cgggaacgac agacccacgt cgatgatcag gatgcggtcg tccagctcga
      601 ggcaggccat gttgcgcccg acctcgccga ccccgccgag gaagacgagt cgcgccgagg
      661 actcagagga gaccggcgtc ctccagtgct ctccggatcc gctcgcgctc gtccggggtc
      721 gcgggaacga gcggcagccg aggcgggccc gccggccgac ccagcatgtc cagggcggcc
      781 ttcacgggga tcgggttcga cgtgacgaac agcgcgttga acaacggaga gagctcctcg
      841 tggagcttcc gcgcggcggg cacgtcgccc gtctcgatca ggtcgatcat ctgcttcatc
      901 tgcagcccga cgagatgcga ggccacgctc acgacaccca cggcgcccag gcagacgtac
      961 ccgaacgccg cccagtcgtc accggaatac acctcgaacc cgtcgggtgc ctcggagatc
     1021 aggttcgaga tggccttgaa gtcgcccgtg gagtccttca ccgcgacgat gttcggcttc
     1081 tcggcgatcc ggagcatcgt ctcgtgctcg atgcgggtac cggtgcggct cgggatgttg
     1141 tagacgatga tcggcagctc cgtcgcttcg gcgatgtgct cgaaatgcgc gacgagaccg
     1201 cgctgcggcg gcttgttgta gtacggcgtg acgacgacga tgccgtctgc gccggcgtcc
     1261 tcggcggcct gggtcagctc gagcgtctcc gccgtggagt aggtgccggt cccgcagatc
     1321 atcttgccct tgccgcggat cgcctccccc accgcgcgga acaggtcggc cttctcgttg
     1381 tgggacaggg tgggcgactc gccggtcgag cccacgacga agacagagtc cgagccgttg
     1441 tcggtgagcc aggacgcgag ctcttgcgcg ccatcgagat cgacgccgtg gtcgggacgg
     1501 aacggcgtga ccatcgcggt gacgacggat ccgaaccttc cggccacgcg aagcctcctt
     1561 ggtctggcgg ccctcgcgat ccgatcggat gcggggcgtc cgcagtggag ccctatgcta
     1621 gctctccatg ccgcgcatct acacgaagac cggcgacgac gggaccaccg ggctcctgta
     1681 cggaggccgg gtccccaagg acgacgcggc caccgaggcg tacgggacca ccgacgaggc
     1741 agtggctgtg ctcgggctgg cccgcgcgtc ggcggagccc gtgatggccg aggagctcct
     1801 cgccctacag cgggagctct tcatcgtggg tgcggatctc gccacgaacc cggccgagcg
     1861 cgcgaagctc cagccgggcg tgtcgctcgt gacgagcgac atgaccgaac gactcgagcg
     1921 gcggatcgat gagctcgtcg ccgagcgagc actccccgac gcgttcatcg ttcccggcgc
     1981 gaacgaagga agcgcggctt tggac
//
LOCUS       DASUVE010000364         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1685735, whole genome shotgun sequence.
ACCESSION   DASUVE010000364 DASUVE010000000
VERSION     DASUVE010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685735"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..987
                     /gene="ppk2"
                     /locus_tag="VF036_07855"
     CDS             103..987
                     /gene="ppk2"
                     /locus_tag="VF036_07855"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEX6265181.1"
                     /translation="MGDSKVKGKQRSLGSPESSTQDLNASPPKVPKKIYTAELLRLQE
                     ELVKMAEWVKASGERVVVIFEGRDAAGKGGAIKRITEYLNPRIARVVALPVPSERQTT
                     QWYFQRYVEQLPAGGEIVLFDRSWYNRAGIEKVLGFCTPEEHRMFLQQCPIFERLLVA
                     DGIHLTKYWFSVSDQEQERRFVDRIDDPMRRWKLSETDMDSRTRWVDYSRAKDEMFVH
                     TDIPEAPWFVVPADDNRRARLNCMAHLLSRLPYVPKTLKELEMPERQDDEGYVRPPRE
                     IYTYVPDHAASVMRAAPS"
     gene            complement(1008..>2260)
                     /locus_tag="VF036_07860"
     CDS             complement(1008..>2260)
                     /locus_tag="VF036_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265182.1"
                     /translation="SRRHTRSGGSWWETPSLRLAAIVGGSVAVGTASMLVAPALPRKI
                     PWVEAERIELKNALRLPALRLPVWAAAATAGAAVSLSATDNRRRMTSVLLIGWSATVV
                     VAALLFRMGWNVPPYRYATFALALPILATLFVTTLATLIARRLPILGTFVGVSLAVAA
                     VVWATSWSVDYWRSQPSIPEEQFDQSLAAGRYLASTGEERPVVFITSLPQIVFPDHML
                     RAGVPPSFVDDVYVFPGTLRELRFLQENPGEELLNPGANRRSQTARELIADVFVRDPI
                     VLFLSAFNEGRRAPPSQEVQHGVFVVQGPSGAIEPAASAGTSDVVGDALALAALLAAV
                     GIGWSVALAPGDLLARTGLAMAFGLALLAVVGTVAGRLGLPLRGASGTALVIATALGG
                     WVVKAIGHLRTSSGRHRRNAVSGG"
BASE COUNT          422 a          751 c          769 g          318 t
ORIGIN      
        1 caggagcttg cccccatctg gtcgaggtca caacccgagt gcgccggcgc ccagaggcac
       61 accgggggtc gatgctctac ggtggtctca gggaggcgcg agatgggcga ctcgaaggtc
      121 aagggaaagc agcgcagcct cggatcaccc gagagttcca cccaagacct caacgcgtcg
      181 cctcccaagg tcccgaagaa gatctacacg gcggagctcc tccggctgca ggaggagctc
      241 gtgaagatgg ccgagtgggt gaaggcgtcc ggcgagcgtg tggtcgtgat cttcgagggc
      301 cgagacgcag cgggcaaggg cggcgccatc aagcgcatca ccgagtatct gaatcccagg
      361 atcgcgcggg tggtcgcgct cccggtgccc tcggaacgac agacgaccca gtggtacttc
      421 cagcggtacg tggagcaact gcctgccgga ggggagatcg tgttgttcga ccgcagttgg
      481 tacaaccgcg ccggcatcga gaaggtgctg gggttctgca cacccgagga gcaccggatg
      541 ttcctccaac agtgtccgat cttcgagcga ctcctcgtcg cggacggcat ccacctgacg
      601 aagtactggt tctccgtgag cgaccaagag caggagcgtc gtttcgtgga tcggatcgac
      661 gacccgatgc gccgctggaa gctctccgag acggatatgg attcgcgcac ccgttgggtc
      721 gactactcac gggccaagga cgagatgttc gtgcacaccg acatcccgga ggcaccgtgg
      781 ttcgtcgtcc ccgccgacga caatcgtcgg gctcgcctca actgcatggc gcacctgctg
      841 agccgactcc cgtacgttcc gaagacgctg aaggagttgg agatgcccga gcggcaagac
      901 gacgagggct acgtgcggcc tccgcgcgag atctacacgt acgtgcccga tcacgccgcg
      961 agtgtgatgc gcgcggctcc ttcgtagaac gctgaagcaa cagccgctca cccgcccgag
     1021 acggcgtttc gccggtgccg gccggaactc gtccggaggt ggccgatcgc cttcacgacc
     1081 cacccaccaa gcgcggtggc gatgacgagc gccgtcccgg aggctccgcg cagcggcagt
     1141 ccaagtctgc ccgctaccgt gccgacgacc gcgaggaggg ccaacccgaa cgccatcgcc
     1201 agtcctgtcc gcgccagcag atccccaggt gcgagcgcga cggaccagcc gatccccacc
     1261 gccgcgagca gggcggcgag tgcgagcgcg tcgccgacga cgtcactcgt accggccgat
     1321 gcagccggct cgatcgcgcc ggaaggtcct tgcacgacga agacgccatg ttgcacctcc
     1381 tgactcgggg gcgcccgacg accctcgttg aaggcgctga ggaatagcac gatcgggtct
     1441 cggacgaaca catcggcgat cagctcacgc gcggtctggg aacgacgatt ggcccctggg
     1501 ttcagcagct cctccccggg attctcctgg aggaagcgga gctctctcag cgtgccgggg
     1561 aacacgtaca cgtcatcgac gaacgagggt ggcacgccgg cacgcagcat gtggtcgggg
     1621 aacacgatct gcgggagaga ggtgatgaag acgacggggc gctcctcgcc ggtggaggcg
     1681 agataccgcc cggcggccag ggactggtcg aactgctcct cagggatcga cggttggctc
     1741 cgccagtagt cgacgctcca cgaggtcgcc cacacgaccg cggcgaccgc caacgacacg
     1801 ccgacgaagg tgccgaggat aggcagtctg cgggcgatca acgtcgccag ggtcgtgacg
     1861 aacaacgtcg ccaggatcgg aagcgccagc gcgaacgtgg cgtaccggta cgggggtacg
     1921 ttccagccca tccggaacag gagggccgcc acgaccacgg tcgcactcca tccgatcaag
     1981 agcacgctcg tcatccgccg gcggttgtcc gtggcggaca gggagacggc cgcaccagcc
     2041 gtcgctgccg ccgcccacac agggagtcgt aatgcgggga gacgaagggc attcttcagt
     2101 tcgatcctct cggcctcgac ccaggggatc ttccgtggga gcgccggcgc gacgagcatg
     2161 gaggcggtcc ccaccgccac gctccccccg acgatcgcgg cgagccggag cgagggcgtc
     2221 tcccaccagg agcctccgct ccgggtgtgt cgacgggacg
//
LOCUS       DASUVE010000365         3354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_179139, whole genome shotgun sequence.
ACCESSION   DASUVE010000365 DASUVE010000000
VERSION     DASUVE010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179139"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1536)
                     /locus_tag="VF036_07865"
     CDS             complement(<1..1536)
                     /locus_tag="VF036_07865"
                     /inference="COORDINATES: protein motif:HMM:NF045524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_6640 family putative metalloprotease"
                     /protein_id="HEX6265183.1"
                     /translation="MRVHATTRAVTSALVASVLVAGPSLAAPEPLGNEASGTSTPVVE
                     PRRRLPALTAPSIPDALSRSLSKGEVTAAVYALERARSLFDLAGVRARFGQVTRPDPR
                     SATLVLRDLALRVGQLSGRPRVTADRILARPTDGAGDPFDDGYTVSEETPECSADVCV
                     HYVAATADAPDPDDLDISGVPDYVETVASVLDHVWAEEVDGLGYRAPKSDATSDNDGG
                     SALLDVYLADIGGGGLYGYCASDDPHLDPPPYDYWDMSAYCVLDDDYDISQFGYANPM
                     RPLKVTAAHEFFHAVQFAYDIGEDGWLMESTATWMEEHVYDAIDDNRQYLATSPLAQP
                     SIPLDRTSGFRVYGTWIFWQFLTEYFGGSVPDASIVRAVWRRADGSQVGPDMYSTKAL
                     AAAVGARTIDGTRWRFRWAFADFAVWNDRPARFYDEGDAYPSAPVSRSVTLTGAAPSM
                     SSTAKLDHLTNRSVVARRGSGLRADARLHITVNGPPSRTGPEATAVVIRKSGTARSKV
                     VALN"
     gene            complement(1575..2282)
                     /locus_tag="VF036_07870"
     CDS             complement(1575..2282)
                     /locus_tag="VF036_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX6265184.1"
                     /translation="MTITIRRDADISDVDGGWFRARWHFSFDTYHDPAYMRFGTLRVF
                     NDDRLVPGAIWPMHPHKDIEGLTYVVEGSFRHQDDVGGAPGPLPAGSVQRMTLGHGAW
                     HSEQNASKTEPMRFIQMWIIPAERGLEPGVEQKVLTEEDRTDRLLRVISGDGGDAVLV
                     HQDAHVFVSRLHPDIDATHALGEGRGVYLYLIDGDADVAGERMTTGDAAEIEDEPSIP
                     IHANAVTELIAVDVALS"
     gene            complement(2307..2540)
                     /locus_tag="VF036_07875"
     CDS             complement(2307..2540)
                     /locus_tag="VF036_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005311426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX6265185.1"
                     /translation="MVSAYILIQTEVGKAAQVTQQVGGIDGVVAVDPVTGPYDVVARA
                     EADDLDGLAKGTVMPMQELDGITRTLTCPVIQL"
     gene            complement(2551..2790)
                     /locus_tag="VF036_07880"
     CDS             complement(2551..2790)
                     /locus_tag="VF036_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265186.1"
                     /translation="MTNNANGSDEQPTTGIVDKAKEVAGTVAEKAGPAMEKAKEVAGT
                     VAEKAGPAMEKAKGSATELVDKAKGFLATKDDDAK"
     gene            complement(2897..>3354)
                     /locus_tag="VF036_07885"
     CDS             complement(2897..>3354)
                     /locus_tag="VF036_07885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6265187.1"
                     /translation="MRLSSRLHRRMGLRKVYALGCLVYATGFLLWGLVESRTIVSLLA
                     VFEGAGFSLLFTTSILVVGKLLPSTLYSTGNSIGAMVGFGIGPIIGAGLGGVVYGRLG
                     STTLFIGSAVLALLGGVAALVALRIQPLDTPLREQVPTGDAVAPEPGRL"
BASE COUNT          509 a         1133 c         1165 g          547 t
ORIGIN      
        1 gtttaacgcg acgaccttgg agcgggcagt gccggacttc cggatcacga cggcagtcgc
       61 ctcgggcccg gtcctggacg ggggaccgtt cacggtgatg tggagcctgg catcggccct
      121 caggcctgac ccccgacgcg ccaccacgga gcggttcgtc aggtggtcga gcttcgccgt
      181 cgagctcatc gacggtgcgg cccccgtgag ggtcacggat ctggatacag gcgccgaagg
      241 gtacgcatcc ccttcgtcgt agaacctcgc cgggcggtcg ttccagaccg cgaagtccgc
      301 gaaggcccat cggaatcgcc agcgcgtccc gtcgatcgtc cgggctccga cggcggcggc
      361 cagcgccttc gtcgaataca tgtccgggcc gacttggctg ccgtccgccc tcctccatac
      421 ggcgcgcacg atgctcgcgt cgggcaccga gcctccgaag tactcggtga ggaactgcca
      481 gaagatccag gtgccgtaca cgcggaagcc ggatgtccga tcgagcggga tcgacggctg
      541 cgccagcggg ctggtggcga ggtactgccg gttgtcgtcg atcgcgtcgt agacgtgctc
      601 ctccatccag gtcgccgtgc tctccatcag ccatccgtcc tcgccgatgt cgtaggcgaa
      661 ctggacggcg tggaagaact cgtgggcggc cgtcactttg agcggccgca tcgggttcgc
      721 gtatccgaat tgtgagatgt cgtagtcgtc gtcgagcacg caatacgccg acatgtccca
      781 gtagtcgtag ggagggggat cgaggtgcgg gtcgtcgctg gcgcagtagc cgtagagccc
      841 accgccaccg atgtcagcga gatagacgtc gaggagcgcg cttcctccgt cgttgtcgga
      901 ggtggcatcc gacttgggcg cgcgatagcc cagaccgtcg acctcttcgg cccagacgtg
      961 atcgagcacg ctcgccaccg tctcgacgta gtcgggcaca ccgctgatgt cgaggtcgtc
     1021 cgggtccggc gcgtcggccg tggcggccac gtagtggaca cagacatccg cggaacactc
     1081 cggggtctcc tccgacaccg tgtacccgtc gtcgaacgga tcgcccgcgc cgtcggtcgg
     1141 acgggcgagg atgcggtcgg cggtcactcg cggccgcccg gagagctgcc cgacgcggag
     1201 ggcgagatct cgcagcacta gcgtggcgga ccggggatcc ggtcgcgtca cctggccgaa
     1261 ccgtgcacgg acccccgcca ggtcgaacag ggatctggcg cgctcgagcg cgtagacagc
     1321 tgcggtgact tcccccttcg agagcgaacg gctcagggca tcggggatcg acggggccgt
     1381 cagggccgga agtcgcctgc gaggctcgac gaccggagtc gacgtacccg acgcctcgtt
     1441 tccgaggggt tccggggctg cgagcgaggg tcccgcgacg agcacggagg cgacgagcgc
     1501 ggatgtgacc gctcgagtcg ttgcatggac gcgcatcggc gcacggtacc cgcgaccgag
     1561 gggcgaagca aggctcagga cagcgccacg tcgacggcga tcagctcggt caccgcgttc
     1621 gcgtggatcg ggatcgaagg ctcgtcctcg atctcggccg cgtcgcccgt cgtcatccgc
     1681 tccccggcga cgtcggcgtc accgtcgatc aggtacaggt agacgcctcg gccctcgccg
     1741 agggcatgtg tcgcgtcgat gtccgggtgc agcctggaga cgaagacgtg tgcgtcctgg
     1801 tggacgagca cggcgtcgcc gccgtcgccg ctgatcaccc tcaggagccg gtcggtgcga
     1861 tcctcctcgg tcaacacctt ctgctcgacc ccgggctcca agccgcgttc cgcggggatt
     1921 atccacatct ggatgaatcg catcggctcg gtcttgctcg cgttctgctc cgagtgccat
     1981 gcgccgtggc cgagcgtcat ccgctgcacg gacccggcgg gcagcggtcc cggcgcgccg
     2041 ccgacgtcgt cctggtgacg gaaggagccc tcgaccacgt aggtgaggcc ctcgatgtcc
     2101 ttgtgcgggt gcatcggcca gatcgcgccg ggcacgagcc ggtcgtcgtt gaacacgcgg
     2161 agcgtgccga accgcatgta cgccgggtca tggtaggtgt cgaacgagaa gtgccagcgc
     2221 gcacggaacc acccgccgtc cacgtcggag atgtccgcgt cgcgccggat cgtgatcgtc
     2281 atcgttctgc gggccgagcc cgcgcgtcag agctggatga ccgggcaggt gagggtgcgg
     2341 gtgatgccgt cgagctcctg catcggcatg accgtgccct tggccagtcc gtcgaggtcg
     2401 tccgcctccg cacgggcgac gacgtcgtag ggtccggtca ccgggtcgac ggccacgacg
     2461 ccgtcgatgc caccgacctg ctgcgtcacc tgcgcagcct tcccgacctc ggtctggatg
     2521 aggatgtagg cgctgaccat cagatccccc tcatttcgcg tcgtcgtcct tggtcgcgag
     2581 gaaccccttc gccttgtcga cgagctccgt tgcggacccc ttggccttct ccatcgctgg
     2641 acctgccttc tcggcgacgg tccccgcgac ttccttggcc ttctccatcg ctggacctgc
     2701 cttctcggcg accgtccccg cgacctcctt cgctttgtcc acgatgccgg tggtcggttg
     2761 ttcgtcggaa ccgttcgcgt tgttcgtcat cggtcatacc tcccggttgg ggtccggccg
     2821 tgggatcggc cggtgctccg gacgctcatc gtgtcacgac ggagtgcttc gctgcaaagc
     2881 gcgcaggtca ggtgcgtcag agccggccgg gctcgggagc gaccgcgtcc ccggtcggaa
     2941 cctgctcccg cagaggggta tcgagtggtt ggatccggag cgcgaccagc gccgcgacgc
     3001 ccccgaggag ggcgaggacc gcggagccga tgaagagcgt cgtggagccg agccgcccgt
     3061 acacgacgcc gcccagcccc gctccgatga tcggaccgat cccgaagccg accatcgcgc
     3121 cgatcgagtt gcccgtcgag tagagcgtgg agggcagcag cttgccgacg acgaggatcg
     3181 aggtggtgaa gagcagcgag aatccggcgc cctcgaagac ggcgagcagc gacacgatcg
     3241 tgcggctctc gacgagaccc cacagcagga acccggtcgc gtagaccagg catccgaggg
     3301 cgtacacctt gcgcaggccc atacgccgat gcaggcgcga ggagagccgc atga
//
LOCUS       DASUVE010000366         2974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1311526, whole genome shotgun sequence.
ACCESSION   DASUVE010000366 DASUVE010000000
VERSION     DASUVE010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311526"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /gene="glmU"
                     /locus_tag="VF036_07890"
     CDS             <1..1271
                     /gene="glmU"
                     /locus_tag="VF036_07890"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX6265188.1"
                     /translation="AKPSRIVVVVGHGADDVREAVESWGIKPAPVFVEQADQLGTGHA
                     VQLAEDAVGKAADVLVANGDFDPVTAADVRKLLSAHRRSGAAVTLGSTILDTPGGYGR
                     VVRDGRRVVDVVEGRDAPPAVRKIHEVATNWIGFRRDLLFATLPLLDRENRQREYYLN
                     RAISILLDKGERVDAVLCDTGGVLGANTRAGLAGLEALIRRRTNDLHMAAGVRLIDPS
                     ATYIDVEVRLGKDTVVYPNTFLETGVRIGAGCRIGPSTAIARSRVGDRSVVWFSVLEH
                     ARVGKDCEVGPFARLRPGTDLQDGALVGNYVEVKGSRMGKGSKAKHLTYIGDAELGAG
                     VNVGAGTVVVNYDGYDKHPTVIGDGARIGSDTMLVAPLKVGKGAVTGAGSVITKDVPA
                     GALAVERSDQRNVKGYRKRADAKHRSKGEH"
     gene            1281..2261
                     /locus_tag="VF036_07895"
     CDS             1281..2261
                     /locus_tag="VF036_07895"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX6265189.1"
                     /translation="MEIITKKRMMLFSGTIHPALGSEIAECLGIPVSHSELRRFASGE
                     IYFRAEESVRGADVFVVQSHYEPVNEALMEQLIMIDAMKRASAKRINAVIPFYGYSRQ
                     DHKALSREPISAKLVADLLSTAGADRVISVDLHSGQIQGYFDFPFDHLTALPILSDYF
                     KEELGLHGDNLVVVAPDAGRIKTSEKLREYLHADLAFLYKRRSRTEAHKIEEMAVVGE
                     VEGRPCVIVDDMIDTAATISKGVQVLAEIGAGPIFVGATHGLFSGKARQLLEEAPIEQ
                     VVVTNTVPIPEERTFAKLKVLSIAPLIANGLRAVFEDASVSEIFGGDNQV"
     gene            complement(2258..2455)
                     /locus_tag="VF036_07900"
     CDS             complement(2258..2455)
                     /locus_tag="VF036_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265190.1"
                     /translation="MRVLHDVADGAALDGLLAGARFHKYLVTDTRLARIDRVVPHPAF
                     RDWLFTPLTEEELRGLELPPD"
     gene            complement(2500..2583)
                     /locus_tag="VF036_07905"
                     /pseudo
     CDS             complement(2500..2583)
                     /locus_tag="VF036_07905"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2562..2819)
                     /locus_tag="VF036_07910"
     CDS             complement(2562..2819)
                     /locus_tag="VF036_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265191.1"
                     /translation="MVAPHVGAHAFEIVPAVADALEANARRNGVDDRVAVHREGIGDP
                     NKTMRVPSGEGGSALPSFYSSRIRFDEARKSTSDRWIPSPP"
BASE COUNT          484 a          999 c         1051 g          440 t
ORIGIN      
        1 ccgcgaagcc ttcgaggatc gtcgtcgtcg tcggccacgg tgcggacgac gtccgcgagg
       61 cggtcgagtc gtggggcatc aagcccgcgc ccgtcttcgt cgagcaggcc gaccagctcg
      121 ggaccggtca tgcggtccag ctcgccgagg acgccgtcgg caaggcggcg gacgtcctgg
      181 tggcgaacgg ggacttcgat ccggtcaccg ccgccgacgt ccggaagctc ctgtccgcgc
      241 atcggcgctc cggcgcggcc gtcaccctcg gttccacgat cctcgacaca cccggcggct
      301 acgggagggt cgttcgcgat ggccggcgcg tcgtcgacgt ggtcgagggc cgcgatgcgc
      361 cgcccgccgt ccggaagatc cacgaggtcg cgacgaactg gatcgggttc cgccgggacc
      421 tgctgttcgc gaccctgccg ctcctcgacc gggagaaccg ccagcgcgag tactacctga
      481 accgggcgat ctcgatcctc ctcgacaagg gcgagcgcgt cgacgccgtc ctgtgtgaca
      541 cgggcggggt gctcggcgcc aacacccggg ccggcctggc cggtctcgag gcgctgatcc
      601 gtcgccggac caatgatctc catatggccg ccggcgtccg cctgatcgac ccctccgcca
      661 cctacatcga cgtcgaggtc cgtctcggca aggacacggt cgtctacccg aacacgttcc
      721 tggagacagg ggtccggatc ggcgccggct gcaggatcgg tccgtccacc gcgatcgccc
      781 ggtcccgcgt cggcgaccgc tcggtcgtgt ggttctcggt cctcgagcac gcccgggtcg
      841 ggaaggactg cgaggtcggt ccgttcgccc ggctgcggcc cggcaccgac ctccaagacg
      901 gagcgctggt ggggaactac gtcgaggtca agggctcccg tatgggcaag ggctccaagg
      961 ccaagcacct cacgtacatc ggcgacgccg agctcggggc gggcgtgaac gtcggtgccg
     1021 ggaccgtcgt cgtgaactac gacgggtacg acaagcaccc cacggtgatc ggcgacgggg
     1081 cgaggatcgg gtcggatacg atgctggtgg cgccgctgaa ggtcggcaag ggcgccgtca
     1141 cgggggccgg atcggtgatc acgaaggacg tgccggcggg cgcgctggcc gtggaacggt
     1201 ccgaccagag gaacgtgaag gggtaccgga agcgcgccga cgccaaacat cggtcgaagg
     1261 gtgagcatta gggggcgacc gtggagatca tcaccaagaa acggatgatg ctgttctccg
     1321 gaacgatcca tccggcgctg gggagcgaga tcgccgagtg tctcggtatc ccggtgagcc
     1381 acagcgagct ccggcgcttc gcgtcggggg agatctactt ccgtgccgag gagagcgtcc
     1441 ggggggccga cgtgttcgtc gtgcagtccc actacgagcc ggtgaacgaa gcgctgatgg
     1501 agcagctgat catgatcgac gccatgaaac gggcgtcggc caagcggatc aatgccgtga
     1561 tcccgttcta cggctactcc cgccaggatc acaaggccct ctcccgcgag ccgatcagcg
     1621 cgaagctcgt cgccgacctc ctgtccacgg cgggcgccga ccgggtgatc tcggtcgacc
     1681 tccactcggg gcagatccag ggctacttcg atttcccgtt cgaccacctc acggcgctcc
     1741 cgatcctgtc ggactacttc aaggaggagc tggggctgca cggggacaac ctggtcgtgg
     1801 tggcgcccga cgccgggcgg atcaagacct cggagaagct ccgcgagtac ctccacgccg
     1861 acctcgcgtt cctctacaaa cgtcgctccc gcacggaggc gcacaagatc gaggagatgg
     1921 cggtcgtcgg tgaggtcgag ggcagaccgt gcgtgatcgt cgacgacatg atcgacaccg
     1981 cggccacgat ctcgaagggc gtccaggtcc tcgccgagat cggcgccggc ccgatcttcg
     2041 tgggggcgac ccacggcctg ttcagcggca aggcgcgtca gctgctggag gaggcgccga
     2101 tcgagcaggt cgtcgtgacg aacaccgtgc cgatccccga ggagcgtacg ttcgccaagc
     2161 tgaaggtcct ctcgatcgca ccgctgatcg cgaacggcct ccgcgcggtc ttcgaggatg
     2221 cctcggtgtc ggagatcttc ggcggcgaca accaggtcta gtcgggcggg agctcgagac
     2281 cgcgcagctc ctcttcggtg agaggggtga acagccagtc gcggaacgcc gggtgcggca
     2341 cgacgcgatc gatgcgcgcg agccgcgtgt cggtgacgag gtacttgtgg aacctcgcac
     2401 cggcgagcag tccatcgagg gccgccccgt cggcgacatc gtggaggact cgcacaggat
     2461 gtgcggacga caggtcctca ggaacgcctg gctgaagcgg aagaccgcgt cctctgttcc
     2521 ctccacgtcg accttcatca ccacacgggc cgcttccggg atcagggcgg cgacggaatc
     2581 cagcgatcgg aagtcgactt ccttgcctcg tcgaaccgga tcctcgacga gtagaacgag
     2641 gggagcgccg agcccccctc gccggacggc acgcgcatcg tcttgttcgg atccccgatg
     2701 ccctcccgat ggaccgcgac gcgatcatcg accccgttcc ggcgcgcgtt cgcctccaac
     2761 gcgtcggcca cggccgggac gatctcgaac gcgtgggcgc cgacgtgcgg cgcgaccact
     2821 cacttcttcg aggcgggcgg gtgcggggag ccggcccgcg cggagcgacg gcaccccttt
     2881 cgagacgagc cggaggaacg cgttgggcac gcggagcgcg aggaagcgct tggcgagcgc
     2941 ttccggctga accagctctg ccgccgggcg tgga
//
LOCUS       DASUVE010000367         3486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1687480, whole genome shotgun sequence.
ACCESSION   DASUVE010000367 DASUVE010000000
VERSION     DASUVE010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687480"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="VF036_07915"
     CDS             complement(<1..1439)
                     /locus_tag="VF036_07915"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEX6265192.1"
                     /translation="MSDVFADPTPEELRRFTEAMPECRITEFGNVNVQTRVVSRSASS
                     TFVVDRPSSGKTMTRDAYDDIARRQDAYVADHDMIRIDGWIGNDPELRTRARLLMEKR
                     YANIAGMQQKLYFPRDDDADPEVQVIYTPGLTAPGYPGDRVIAVDLDANVTRVLNSDY
                     FGESKKGGLRMWNNIVFEKGGLALHAGLKVIPTSTGDKVFMIIGLSGTGKTTTTFTTQ
                     NGSKPIQDDFVGLMPGGRAFGTENGCFAKTFSLDPEFEPSIYGAVSKPTTYLENAFQD
                     DSGAVNFFEESYTQNGRAVFEMRDLQTFEDARNVGPVDYLLILNRNENIIPSVAKLNQ
                     EQAAAYFMLGETTGTSAGGAAEEGKFLRVPGTNPFFPLPHGLQGNRLLDLLATHPIET
                     YLLNTGRVGGKDGDDRSKKVKIPHTSACVKAIAEQTIVFEEDPDFRYQVASEVPGFDD
                     RELLRPVLLYEREGRIEEYRTIVERLKVER"
     gene            1589..2548
                     /gene="thiL"
                     /locus_tag="VF036_07920"
     CDS             1589..2548
                     /gene="thiL"
                     /locus_tag="VF036_07920"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX6265193.1"
                     /translation="MDLTEDELIAAIGKVLSGAGPEVRVGPGDDAAVVRGGSGELVLT
                     TDAMVEGVHFLTDRTTPRDLGYKAIAVNVSDIAAMAGSPRFALCALTLTDAIDASWVV
                     ELAGGMRECCDEFAVSLVGGNLSRGEELAIVVTVTGEVGPARAVRRDGARPGDRVVVT
                     GSLGGSAAGLRVSSQRSWSDDERDALRRSMRPVPRVGEASILSAHGATAMIDVSDGLT
                     IDLSRLCRASGVGARVEVGHVPVHPASTVHEALGGGEDYELLATLPGSDAVASATKDV
                     HEGFGVSLSDIGVIIEGESGGLVGVDEDGTERPLAIEGWDHFR"
     gene            2545..3402
                     /gene="thiD"
                     /locus_tag="VF036_07925"
     CDS             2545..3402
                     /gene="thiD"
                     /locus_tag="VF036_07925"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005479870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX6265194.1"
                     /translation="MTDGQSQRRGAPRARALTIAGSDSGGGAGIQADLKTFSALGVFG
                     MTAITAVTIQNTKGVAGFEELSPATVAEQIRAVAGDIGVDAAKTGMLASAAIVEAVAE
                     SATEMSLPNIVVDPVFVSKHGHPLLADDAVDALRRSILPLATLVTPNLAEAAGLAGFE
                     VVTRDDMRRAATTILALGPRAVLVKGGHLEGDRATDLFVSGEREEWLEADRIDTPHTH
                     GTGCTLSSAITAHLARGASLADAVREGKAFVTEAIRHALPLGDGIGPVDQLWSIAVAR
                     EESPEGAER"
BASE COUNT          524 a         1148 c         1233 g          581 t
ORIGIN      
        1 cgctcgacct tcagccgctc gacgatcgtg cggtactcct cgatgcggcc ctcccgctcg
       61 tagaggagga ccgggcgaag gagctcccgg tcgtcgaacc ccgggacctc ggaggccacc
      121 tgatagcgga agtccggatc ctcctcgaac acgatcgtct gctcggcgat cgccttcacg
      181 cacgccgacg tgtgcgggat cttcaccttc ttcgagcggt cgtcgccgtc tttcccaccg
      241 accctcccgg tgttcaacag gtaggtctcg atgggatggg tcgcgaggag atcgagcagg
      301 cggttcccct gcaggccgtg ggggagcggg aagaaggggt tggtgcccgg cacgcgaagg
      361 aacttgccct cctctgcggc gccgcccgcc gacgttcccg tcgtctctcc gagcatgaag
      421 tacgcggccg cctgttcctg gttgagcttc gcgacggacg ggatgatgtt ctcgttccga
      481 ttgaggatca gcaggtagtc gaccgggccg acgttccgcg cgtcctcgaa cgtctgcagg
      541 tctcgcatct cgaagacggc ccgaccgttc tgcgtgtagc tctcctcgaa gaagttcacc
      601 gcgcccgagt cgtcctggaa cgcgttctcg aggtacgtgg ttggtttgct gacggcgcca
      661 tagatggacg gctcgaactc cggatcgagc gagaacgtct tggcgaagca accgttctcc
      721 gtgccgaatg cgcggcctcc aggcatcagt cccacgaagt cgtcctggat gggcttcgag
      781 ccgttctgcg tcgtgaacgt cgtcgtcgtc ttccccgttc cggacagacc gatgatcatg
      841 aacaccttgt cgccggtcga cgtcgggatc accttcagcc ctgcgtgcag agcgaggccg
      901 cccttctcga acacgatgtt gttccacatc cgcagcccgc ccttcttcga ctcaccgaag
      961 tagtcggagt tgaggacgcg cgtgacgttc gcgtcgaggt cgaccgcgat cacccggtca
     1021 ccgggatacc cgggcgcggt caggccggga gtgtagatca cctggacctc cgggtcggcg
     1081 tcgtcgtcgc gagggaagta cagcttctgc tgcatcccgg cgatgttcgc gtaccgcttc
     1141 tccatgagca ggcgggcgcg ggtccggagc tcggggtcgt tcccgatcca accgtcgatc
     1201 cggatcatgt cgtgatcggc cacgtacgcg tcctgtcgtc gggctatgtc gtcataggcg
     1261 tcgcgggtca tcgtcttgcc gctggacggc cgatcgacga cgaaggtgct gctggcgctg
     1321 cgggacacca ctcgggtctg gacgttcacg ttgccgaact cggtgatccg acattccggc
     1381 atcgcttcgg tgaagcgacg gagctcctcc ggggtcggat cggcgaacac gtctgacact
     1441 cggtcctcct ggtccttggt gatcctcggc tggggtcggt tgggaacgcg gaaggcgtgg
     1501 gcaagcggaa agcctagagt ctagcgaacg cccaagctgc ctcctccaga attggaaccg
     1561 gccgggcgga acggccagac ctgagcagat ggacctcacc gaggacgaac tgatcgccgc
     1621 gatcggcaag gtgctctcgg gcgccgggcc cgaggtccgc gtgggaccgg gtgacgacgc
     1681 cgccgtcgtt cggggcggtt cgggagagct cgttctgacg acggacgcca tggtcgaagg
     1741 ggtgcacttc ctcacggatc ggaccacgcc ccgcgatctc ggctacaagg cgatcgccgt
     1801 gaacgtgagc gacatcgccg cgatggcggg gagcccgcgg ttcgcactct gcgcgctgac
     1861 cctcaccgac gcgatcgacg cctcgtgggt cgtcgagctg gccgggggga tgcgggagtg
     1921 ctgcgacgag ttcgcggtgt cgctcgtggg cgggaacctc tcgcgcggtg aggagctcgc
     1981 gatcgtcgtg accgtgaccg gcgaggtcgg accggcgcgc gccgtgcgcc gggacggcgc
     2041 ccgacccggc gaccgggtgg tcgtgacagg gtccctcggc ggctcggccg ccgggctgcg
     2101 ggtctcgtcg caacgctcct ggtccgacga cgagcgtgac gcgctccgcc gctcgatgcg
     2161 ccccgtcccg agggtcggcg aggcgtcgat cctgtctgcc cacggcgcca ccgcgatgat
     2221 agacgtctcc gacggcctca cgatcgacct gtcccggctc tgccgggcga gcggggtcgg
     2281 ggctcgcgtc gaggtcgggc acgtgccggt gcacccggcc tcgaccgtgc acgaggcgct
     2341 cggcgggggg gaggactacg agctgctcgc gacccttccc ggctccgatg cggtcgccag
     2401 cgcaacgaag gacgtccatg aggggttcgg cgtgtcgctc agcgatatcg gcgtcatcat
     2461 cgaaggcgag agcggagggc tcgtgggcgt ggacgaggac ggaacggaac gccccctcgc
     2521 catcgaaggg tgggatcact tccgatgacc gatggacagt cccaacgacg gggagctcct
     2581 cgagcgcggg cgctgacgat cgccggatcc gactccggtg gtggcgcagg gatccaggcc
     2641 gacctcaaga cgttcagcgc gctcggagtg ttcggcatga cggcgatcac ggcggtcacc
     2701 atccagaaca cgaagggagt cgcaggattc gaggaactct cacccgcgac ggtggccgag
     2761 cagatccgtg ccgtggccgg tgacatcgga gtcgacgcgg ccaagacggg gatgctcgcc
     2821 tcggccgcca tcgtcgaggc cgtggcggag tcggccacgg agatgtcgct cccgaacatc
     2881 gtcgtcgacc ccgtcttcgt gtcgaagcac gggcatccgt tgctcgccga cgacgccgtc
     2941 gacgccctcc ggcgatcgat cctgcccctc gcgacgctcg tcacaccgaa cctcgccgag
     3001 gcggcggggc tggccgggtt cgaggtcgtg acccgcgacg acatgcggcg tgccgcgacg
     3061 acgatcctcg cgctcggacc ccgcgccgtc ctcgtgaaag gagggcacct cgagggcgac
     3121 cgcgcgaccg acctgttcgt cagcggggag cgggaggagt ggctcgaggc cgatcggatc
     3181 gacacgccgc acacgcacgg caccggctgc acattgtcct ccgcgatcac cgcgcacctg
     3241 gcccgagggg cgtcgcttgc cgacgcggtc cgcgagggga aggcattcgt cacggaggcg
     3301 atccgccacg ccctcccgtt gggtgacggg atcggtccgg tcgaccagct gtggtcgata
     3361 gctgtcgcgc gtgaggagag ccctgaaggc gctgagcgct aggactggcg ctgcgggatg
     3421 cgggtgacgc ggccggcctt gatgcagctc gtgcacgcgt tgatgcgacc gggcgtgccg
     3481 tcgacg
//
LOCUS       DASUVE010000368         3237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_560339, whole genome shotgun sequence.
ACCESSION   DASUVE010000368 DASUVE010000000
VERSION     DASUVE010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560339"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1859
                     /locus_tag="VF036_07930"
     CDS             45..1859
                     /locus_tag="VF036_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016909605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX6265195.1"
                     /translation="MESVVVRFAGDSGDGMQLTGDRFTSATAVLGNDLATLPDFPAEI
                     RAPAGTVHGVSAFQIQFASNDITTPGDHADVLVAMNPAALKADLDKVGKGGVLILNED
                     SFTERNVQKAGYTADPLQDGTLDGYQVYKVPMTSITVRATEGIGIGKKESERAKNMFA
                     LGLVSWMFGRPTDTTLNWLEKKFGSRQEIFDANVAAFKAGYNFGETTELFAESYKVEA
                     APQVPGTYRNNDGATALAWGLIAASERSGLPLFYASYPITPASELLHELSRHKNFGVV
                     TIQAEDEIAAANIALGAAFSGQLAVTGTSGPGIDLKAETIGLAVIMELPMIVVDVQRA
                     GPSTGMPTKTEQADLLLAMYGRHGESPLPIVSASTPAECFTAALEAARIAVRYRTPVI
                     LLSDTFIANSSEPWPIPDVESLPSIDPGFASPNGQDFLPYSRDERLARPWAIPGTPGL
                     VHRIGGLERQDGTGNISYDAANHELMTRLRQAKVDGIADDIPVLEVEDPSGEAELLVL
                     GWGSSLGTITAAVARARADGRSVATAHLRHLNPFPSNTGEVVRAYRKVLIPEMNMGQL
                     AALIRARYLVDAASYTKVQGLPIFAEELEHEITRVLDE"
     gene            1852..2895
                     /locus_tag="VF036_07935"
     CDS             1852..2895
                     /locus_tag="VF036_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009327591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX6265196.1"
                     /translation="MSDVIENGGSAPGDASTLTKKDFTSDQEPRWCPGCGDYAILSAV
                     QAFMPELGIEPHKTVFVSGIGCSGRFTYYMDTYGMHGIHGRAPALATGIAVAAPELSV
                     WVVTGDGDGLSIGGNHLIHALRRNVDIKILLFNNQIYGLTKGQYSPTSEEGKVTKSTP
                     FGSVDHPFNPIALALGVEATFVARTVDNDRQHLTEVLRRAAQHKGTAFVEIYQNCNVF
                     NDGAFDLLRDKAQGKHNQIRLEHGEPIVFDEGNRCVRVGENGRYEIAPTAETDPDSVV
                     VHDAHGRPSLAFALAHLSRGPDEPSCIGVFHEVERPVYGQAIQHQLQAAKERLGAGDL
                     GSLLHVGDTWTVE"
     gene            complement(2933..>3237)
                     /locus_tag="VF036_07940"
     CDS             complement(2933..>3237)
                     /locus_tag="VF036_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265197.1"
                     /translation="LVGFSVAGLDPATLLMTLDDRGFRLGAGSLCSGRPEDPSPVLEQ
                     IGYPGTTGFRVSLGDGADRSHLDALVGMLSGTVRELRQMEAASAEALSRFSPPATG"
BASE COUNT          565 a         1117 c         1099 g          456 t
ORIGIN      
        1 cgacagcgac cgcagaaaaa ctcacctctt cgcatcgcga gaaactggaa tccgtcgtcg
       61 tacggttcgc gggcgactcg ggcgacggca tgcagttgac cggcgaccga ttcacgagcg
      121 ccacggcggt gctgggcaac gacctcgcca cgctgcccga cttccccgct gagatccgcg
      181 cccccgcggg caccgtccac ggggtctccg ccttccagat ccagttcgca tcgaacgaca
      241 tcaccacgcc cggcgatcac gccgacgtgc tggtcgcgat gaacccggcg gccctcaagg
      301 cggacctcga caaggtcggg aagggcggcg tgctcatcct gaacgaggac tccttcacgg
      361 agcgcaacgt ccagaaggcg ggatacacgg ccgaccccct ccaggacggg accctcgacg
      421 gctaccaggt ctataaggtg ccgatgacct cgatcacggt gcgggcgacg gagggcatcg
      481 ggatcggaaa gaaggaatcg gagcgggcga agaacatgtt cgcgctcgga ctcgtgtcgt
      541 ggatgttcgg tcgcccgacg gacacgacgc tcaactggct ggagaagaag ttcggttccc
      601 gccaggagat cttcgacgcc aacgtcgcgg ccttcaaggc cggctacaac ttcggcgaga
      661 cgacggagct gttcgccgag tcgtacaagg tggaggccgc gccccaggtg ccggggacct
      721 accgcaacaa cgacggcgcc accgctctcg cgtgggggct gatcgccgcg tcggaacgca
      781 gcggtctgcc cctgttctac gcgagctacc cgatcacgcc ggcctccgag ctcctccacg
      841 agctgtcacg ccacaagaac ttcggcgtgg tcacgatcca ggcggaggac gagatcgccg
      901 ccgccaacat cgccctcggc gcggcgttca gcggccagct ggccgtgacc gggacgagcg
      961 ggccgggcat cgacctgaag gccgagacga tcgggctcgc ggtgatcatg gagttgccga
     1021 tgatcgtcgt cgacgtgcag cgtgcgggcc cctcgaccgg catgccgacg aagaccgagc
     1081 aggcggacct gctgctcgcg atgtacggac ggcacgggga atcgccgctg ccgatcgtgt
     1141 cggcatccac tccggccgag tgcttcaccg ccgcgctcga ggcggcccgg atcgccgtac
     1201 ggtaccggac gcccgtgatc ttgctctccg acacgttcat cgcgaactcc tcggagccgt
     1261 ggccgatccc cgacgtcgag agcctgccct cgatcgatcc gggattcgcc tccccgaacg
     1321 ggcaggactt cctcccgtac tcacgcgatg agcggttggc gcggccctgg gcgatcccgg
     1381 ggacaccggg actggtgcac cggatcggtg gattggagcg ccaggacggg accggcaaca
     1441 tcagctacga cgcggccaac cacgagctga tgaccaggct ccgccaggcg aaggtcgatg
     1501 gcatcgcgga cgacatcccg gtcctcgagg tcgaggaccc gagcggcgag gccgagctgc
     1561 tcgtgctcgg ctggggctcc tccctgggca cgatcacggc ggccgtggcc cgagcgaggg
     1621 cagacggccg gagcgtggcg accgcgcatc tccggcatct gaacccgttc ccctcgaaca
     1681 ccggagaggt cgtgcgtgcg taccgcaagg tgctgatccc ggagatgaac atgggacagc
     1741 tcgccgcgct gatccgtgcc aggtacctgg tggacgcggc gtcgtatacg aaggtgcagg
     1801 gcctcccgat cttcgcggag gagctcgagc acgagatcac gagggtgctc gatgagtgat
     1861 gtgatcgaga acggcggctc cgcaccgggc gacgcctcga cgctgaccaa gaaggacttc
     1921 acgtcggacc aggagcctcg ctggtgcccc gggtgcggcg attacgcgat cttgtccgcg
     1981 gtccaggcgt tcatgccgga gctcgggatc gagccgcaca agacggtgtt cgtgtccggg
     2041 atcggctgca gcggccggtt cacgtactac atggacacgt acggcatgca cggcatccac
     2101 gggcgcgccc ccgccctcgc caccgggatc gcggtcgcgg ccccggagct ctcggtgtgg
     2161 gtcgtcaccg gggacggcga cggcctctcg atcggcggga accacctgat ccacgcgctc
     2221 cgtcggaacg tcgacatcaa gatcctcctc ttcaacaacc agatctacgg attgacgaaa
     2281 ggccagtact cgcccacgtc ggaagagggg aaggtcacga agtccacgcc gttcggctcc
     2341 gtcgaccacc cgttcaaccc gatcgcgctc gccctcggcg tcgaggcgac gttcgtggca
     2401 cgcacggtcg acaacgaccg gcagcatctg acggaggtcc tccgacgggc cgcccagcac
     2461 aagggaacgg ccttcgtcga gatctaccag aactgcaacg tcttcaacga cggcgcgttc
     2521 gacctgttgc gcgacaaggc ccagggcaag cacaaccaga tccggctcga gcacggcgag
     2581 ccgatcgtgt tcgacgaggg gaaccgatgc gtccgtgtcg gcgagaacgg caggtacgag
     2641 atcgcgccca cggccgagac agacccggac agcgtggtcg tgcacgacgc ccacggccgg
     2701 ccatcgctcg cgttcgcgct ggcccacctc tcgcgcggcc cggacgaacc ctcgtgcatc
     2761 ggtgtcttcc acgaggtgga gcgcccggtg tacgggcagg cgatccagca tcagctccaa
     2821 gccgcgaagg agcgcctcgg ggcgggcgac ctcggctcgc tcctccacgt cggggacacc
     2881 tggacggtgg agtagcggcc cggcggccgg aggggcctgc ccggatctcg gctcagccgg
     2941 tggccggcgg actgaaccgg gagagtgcct ccgcggaggc cgcctccatc tgccggagct
     3001 cgcgcacggt cccggagagc atccccacga gggcatcgag gtgggatcgg tccgcaccgt
     3061 ccccgagact cacccggaat ccggtcgtcc ccgggtagcc gatctgctcc aggaccggcg
     3121 acgggtcctc gggacgtccg ctgcagagcg atccggctcc gaggcggaag ccgcgatcgt
     3181 ccagcgtcat cagcagcgtc gcggggtcga ggcccgcgac gctgaacccc accaggt
//
LOCUS       DASUVE010000369         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_935326, whole genome shotgun sequence.
ACCESSION   DASUVE010000369 DASUVE010000000
VERSION     DASUVE010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935326"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..166)
                     /locus_tag="VF036_07945"
     CDS             complement(20..166)
                     /locus_tag="VF036_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265198.1"
                     /translation="MSGIRWVVVVLLVLVLVGLLAFARGREHHRGDEEGALGARVAAV
                     RVEV"
     gene            complement(178..1440)
                     /locus_tag="VF036_07950"
     CDS             complement(178..1440)
                     /locus_tag="VF036_07950"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEX6265199.1"
                     /translation="MTEFGVHSEVGKLHKVMVHRPELSLQRLTPTNHDELLFDDVLWV
                     ERAQYEHDQFVEKMRGRGVEVFLFRDLLAEALAASEEARKRLIEVAVTEYTVGVSLVD
                     EVRSTLGNMKPDELAKHLIGGLTVAEAGVDLGKFREFSLPAAALDDTSAFILPPLPNT
                     LFTRDSSCWIYGGVSINPMFWPARRLEAYNVAAIYRYHPMFQDDDFEYWYPPLGDDGR
                     FQAADFGRASMEGGDVQPIGNGTVLIGLSERTQARMIEQIARALFAKGAAERVIAAVM
                     TKDRAHMHLDTVFTLLDRDKVTAYPKVIDQVRAISLRPGSSEGAFHVTEEEGFIPAVA
                     DALGVSDLTVVETGGNEYQQEREQWDDGNNVVAVEPGVVVAYERYTYTISKMGEAGVE
                     VITIEGFELGKGRGGGHCMTCPLVRDPI"
     gene            complement(1469..>2240)
                     /locus_tag="VF036_07955"
     CDS             complement(1469..>2240)
                     /locus_tag="VF036_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX6265200.1"
                     /translation="GNGPQVGLLALQAASYEEVEAYPLDVLGAQTEGMIGYMLEQELG
                     NLLPAEVPFATILTMVEVDPDDPAFGDPTKFVGPVYEKAETDELADQKGWAFKQDGEK
                     WRRVVPSPEPKRIFEIRPIRWLLEKNAVVICAGGGGVPTMFSKGAERTLVGVEAVIDK
                     DLASELLAREVDAELFVMATDVDGVYAGWGTPDQRRLDEVTVDELRAGEFAAGSMGPK
                     VGAAIRFVERTGKRAAIGGLGDIETIVEGSGGTQVVPG"
BASE COUNT          345 a          837 c          693 g          365 t
ORIGIN      
        1 ttgtcggcca tgggacccgc tacacctcga cgcggaccgc ggcgacgcgt gcgccgagtg
       61 cgccctcctc gtcgccgcgg tgatgctcgc ggccgcgagc gaacgcgagt agcccgacga
      121 ggaccagcac gaggagcacg acgacgaccc accggatccc gctcatccaa cccccttcta
      181 gatcggatcg cggaccagcg gacacgtcat gcagtgtccg ccgccgcgac ccttgccgag
      241 ctcgaacccc tcgatcgtga tcacctcgac cccggcctcc cccatcttcg agatcgtgta
      301 cgtgtaccgc tcgtacgcga ccacgacgcc gggctcgacc gcgacgacgt tgttgccgtc
      361 gtcccactgc tcccgctcct gctggtactc gttgccgccc gtctcgacca ccgtgaggtc
      421 ggagacgccg agggcgtcgg cgaccgcggg gatgaagccc tcctcttccg tcacatggaa
      481 cgcgccctcc gacgatcccg ggcggaggct gatcgcccgg acctggtcga tcaccttcgg
      541 gtacgccgtg accttgtccc ggtcgagcag ggtgaacacg gtgtccaggt gcatgtgcgc
      601 ccggtccttg gtcatcacgg ccgcgatcac gcgctcggcc gcgcccttgg cgaacagtgc
      661 cctcgcgatc tgttcgatca tccgggcctg ggtccgctcg gagagaccga tcagcacggt
      721 gccgttcccg atcggctgca cgtccccgcc ttccatcgag gctcggccga agtccgcggc
      781 ctggaaccga ccgtcgtccc cgagcggcgg ataccagtac tcgaagtcat cgtcctggaa
      841 catcgggtgg tagcgataga tcgcggcgac gttgtaggcc tccaggcgac gcgcgggcca
      901 gaacatcggg ttgatcgaga cgccgccgta gatccagcac gacgagtcgc gcgtgaacaa
      961 cgtgttcggg agcggcggca ggatgaaggc gctcgtgtca tcgagcgccg ccgccggcaa
     1021 cgagaactcc cggaacttcc cgaggtcgac acccgcctcc gccacggtga ggccgccgat
     1081 caggtgcttg gcgagctcgt cgggcttcat gttcccgagg gtgctccgga cctcgtccac
     1141 cagggatacg cccaccgtgt actcggtgac cgcgacctcg atcagtcgct tccgggcctc
     1201 ctcactcgcg gccagtgcct cggccagcag gtcccggaac aggaagacct ccacgcctcg
     1261 cccgcgcatc ttctcgacga actggtcgtg ctcgtactgc gcccgttcga cccacagcac
     1321 gtcgtcgaac agcagctcgt cgtggttggt cggggtcagc cgctggaggc tgagctccgg
     1381 acgatgaacc atcaccttgt gcagcttgcc cacctcggaa tggacaccga actccgtcat
     1441 gtcgtcgcct ccgttccctg tgcggggatc acccgggcac cacctgggtg ccgccggacc
     1501 cctccacgat cgtctcgata tcccccaggc caccgatcgc ggcccgcttc ccggtccgct
     1561 cgacgaaccg gatcgccgcc ccgaccttgg gacccatcga tccggcagcg aactcgcccg
     1621 cccggagctc gtcgaccgtg acctcgtcca ggcgccgctg atccggcgtt ccccagccgg
     1681 cgtagacacc gtccacgtcg gtggccatca cgaagagctc ggcgtcgacc tcgcgagcga
     1741 ggagctcgct cgcgaggtcc ttgtcgatca cggcctcgac accgaccagg gtccgttcgg
     1801 cccccttcga gaacatcgtg gggaccccgc cgccgccggc gcagatcacc acggcgttct
     1861 tctccagcag ccatcggatc ggccggatct cgaagatccg cttgggctcc ggagacggca
     1921 cgacccggcg ccacttctca ccgtcctgct tgaaggccca gcccttctgg tcggcgagct
     1981 cgtcggtctc ggccttctcg tagaccggcc cgacgaactt cgtgggatcc ccgaacgccg
     2041 gatcgtcggg atcgacctcg accatcgtca ggatcgtcgc gaacggaacc tcggccggca
     2101 gcaggttgcc gagctcctgc tcgagcatgt agccgatcat cccctcggtc tgcgcgccga
     2161 gcacgtcgag cgggtaggcc tcgacctcct cgtacgaggc cgcctggagc gcgaggagtc
     2221 cgacctgggg accattgccg
//
LOCUS       DASUVE010000370         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_182139, whole genome shotgun sequence.
ACCESSION   DASUVE010000370 DASUVE010000000
VERSION     DASUVE010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182139"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VF036_07960"
     CDS             <1..208
                     /locus_tag="VF036_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265201.1"
                     /translation="AVVKAKEVGGTVAEVTGPIVHKAKEVAVTLLDKVGPAVEKAMES
                     ATEFVENAVGKAKEFLSTEDDDAK"
     gene            237..470
                     /locus_tag="VF036_07965"
     CDS             237..470
                     /locus_tag="VF036_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX6265202.1"
                     /translation="MVSAYILIQTEVGKAAQVTQQVSGIDGVVAVDPVTGPYDVVARA
                     EADDLDGLAKGTVMPMQELDGITRTLTCPVIQL"
     gene            495..1202
                     /locus_tag="VF036_07970"
     CDS             495..1202
                     /locus_tag="VF036_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX6265203.1"
                     /translation="MTITIRRDAEISDVDGGWFRARWHFSFDTYHDPAYMRFGTLRVF
                     NDDRLVPGAIWPMHPHRDIEGLTYVVEGSFRHQDDVGGAPGPLPAGSVQRMTLGNGAW
                     HSEQNASETEPMRFIQMWIMPAERGLVPGVEQKVFTEEDRTDRLLKVISGDGGDAVLV
                     HQDAHVFVSRLHAAIDATHALGDGRGLYLYLIEGDADVAGERMTTGDAAEIEDEHSIP
                     IHANEVTELIAVDVALS"
     gene            complement(1203..>2188)
                     /locus_tag="VF036_07975"
     CDS             complement(1203..>2188)
                     /locus_tag="VF036_07975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6265204.1"
                     /translation="TQRERYETDSGFRELGYLILATDETQEREARARVEMQRAAGLGD
                     VRYVDAAEAARLNPTLDPATFRGGTYAPGDGCVDPPRNVRAYSIAMQRAGVELRERTS
                     FLGIRTGSDGGVTGVETPEGTIATERVLLTGGPKLREVGALVNARFFVGAVRHQVAVT
                     EPHAAFDVERQPMVFENAIGLYWRLEEGGLLFGMSNPEEAPGKAREVDWPYLQAMEER
                     MHRYVPIARGLGIRKAWAATIEYCPDHLPIVGPVSTRAGEEIPGATVATAVGHGMMWG
                     PGVAKIGADLALVGTTEVADVSGWSMDRFDEEGRSPSYDPIALPFPVSADD"
BASE COUNT          341 a          765 c          749 g          333 t
ORIGIN      
        1 cgcggtagtg aaggccaagg aggtcggcgg caccgtcgcc gaggtgaccg gtccgatcgt
       61 ccataaggcc aaggaggtcg ccgtcacgct cctcgacaag gtcggccccg ccgtggagaa
      121 ggctatggag tccgcgaccg agttcgtcga gaacgccgtc ggcaaggcga aggaattcct
      181 ctcgaccgag gacgacgacg cgaaatgagg gggatctgcc aaggggggat caactgatgg
      241 tcagcgccta catcctcatc cagacggagg tcgggaaggc tgcgcaggtg acgcagcagg
      301 tcagtggcat cgacggcgtc gtcgccgtcg acccggtgac cggtccctac gacgtcgtcg
      361 cccgtgcgga ggcggacgac ctcgacggat tggccaaggg caccgtcatg ccgatgcagg
      421 agctcgacgg catcacccgt accctcacct gcccggtcat ccagctctga cgggcgggct
      481 cggcccgcgg aacgatgacg atcacgatcc ggcgcgacgc ggagatctcc gacgtggacg
      541 gtgggtggtt ccgtgcgcgc tggcacttct cgttcgacac ctaccatgac ccggcgtaca
      601 tgcggttcgg cacgctccgg gtgttcaacg acgatcggct cgtgcccggc gcgatctggc
      661 cgatgcatcc gcacagggac atcgagggcc tcacctacgt ggtcgagggt tccttccgtc
      721 accaggacga cgtgggcggc gcgccgggac cgctgcccgc cgggtccgtg cagcggatga
      781 cgctgggcaa cggcgcatgg cactcggagc agaacgccag cgagaccgag ccgatgcggt
      841 tcatccagat gtggatcatg cccgcggaac gcggcttggt gcccggtgtc gagcagaagg
      901 tgttcacgga ggaggatcgc accgaccggc tcctgaaggt gatcagcggc gacggcggcg
      961 acgccgtgct cgtccaccag gacgcgcacg tcttcgtctc caggctgcac gcggccatcg
     1021 acgcgacgca tgcactcggg gatggccgag gcctctacct gtatctgatc gagggtgacg
     1081 ccgacgtcgc cggggagcgg atgacgaccg gcgacgcggc cgagatcgag gacgagcatt
     1141 cgatcccgat ccacgcgaac gaagtgaccg agctgatcgc cgtcgacgtc gcgctgtcct
     1201 gatcagtcat cggcgctgac ggggaacggg agcgcgatcg gatcgtagga gggcgaacgg
     1261 ccctcctcgt cgaatcgatc catgctccag ccggagacgt cggcgacctc ggtcgtaccc
     1321 acgagcgcga ggtcggcccc gatcttcgcc acgcccggtc cccacatcat cccgtgtccg
     1381 acggcggtgg cgaccgttgc gccggggatc tcttcgccgg ctcgcgtgga gaccggcccc
     1441 acgatcggga ggtggtccgg gcagtactcg atcgtcgcgg cccacgcctt gcggatcccg
     1501 agacctcgcg cgatcgggac gtatcggtgc atccgctctt ccatcgcctg caggtacggc
     1561 cagtcgacct cacgcgcctt gccgggcgcc tcctccgggt tggacatccc gaacaggaga
     1621 ccgccctcct cgagtcgcca gtacaggccg atcgcgttct cgaacaccat cggctgccgc
     1681 tccacgtcga aggctgcgtg gggctccgtc acggccacct ggtgccggac cgcgccgacg
     1741 aagaagcggg cgttcacgag cgcgccgacc tcgcggagct tcgggccccc ggtgaggagg
     1801 acccgctccg tcgcgatcgt gccctcgggc gtctcgactc ccgtcacccc cccgtccgag
     1861 cccgtgcgga tccccaggaa cgatgtccgt tcccggagct ccacgccggc tcgctgcatc
     1921 gcgatcgagt acgcgcgcac gttcctcggc gggtccacgc atccgtcccc cggcgcgtac
     1981 gtcccgccgc ggaacgtggc cgggtcgagc gtggggttca gccgcgcggc ctcggcggcg
     2041 tcgacgtacc ggacgtcccc gagaccggcc gcccgctgca tctccacgcg ggccctcgcc
     2101 tcgcgctcct gcgtctcgtc cgtcgcgagg atcaggtagc cgagctcacg gaagccgctg
     2161 tcggtttcgt accgctctcg ttgcgttc
//
LOCUS       DASUVE010000371         3756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_371725, whole genome shotgun sequence.
ACCESSION   DASUVE010000371 DASUVE010000000
VERSION     DASUVE010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371725"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1374
                     /locus_tag="VF036_07980"
                     /pseudo
     CDS             143..1374
                     /locus_tag="VF036_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019853610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
     gene            1636..>3756
                     /locus_tag="VF036_07985"
     CDS             1636..>3756
                     /locus_tag="VF036_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265205.1"
                     /translation="MRAERHRSIRRSLVAVSALLLVITLTPWAGADELYEGDVPGGVD
                     VQLLTDPVHGLELTASTDVSVASAANPDLEIFDAAGSSVGTYATDGVGGLAISDDGSV
                     LFATKPNEPAIRRLDLTDVPPTELADLAITDVFQPHSVVFAGGALWVAQCLDDSTVEK
                     VDPTTGEETAQDITGLDISRCPQFVDHPTLANVFYAWGRFQGTLQRFDVSSGSLVATA
                     SWPIPGGGAISDVAVAPGGGTLIAAVSFASPSPGAVVLDPTTLVATGTRYRDGTNLSG
                     VSIAADGHVAVGFGTTVEVFASGGASPTATWSSAAPCRVRQITYRGVDFGPSDDVLFV
                     KDQEGPSVAMLSNPTSTLATSTISVSAEPNPGAPGDPVVLSGALDVGGAADSGRTVEL
                     FHSGVSIDSTTTTTGGAFSFDVAPPTAGAQCFDVRFEGISTATGAWSSVTVDVLKIPS
                     SVVINGPLTAYPSESIEFSGTLAFDDDASAEGEIVHVGRSQDGFTFTPAGDAVVEADG
                     TWSITTNAPAVGTWIYRAAYDGTDRYANDLETTQLQVERRPTNLTIGASREQLIFGQS
                     VTLTARLDLLPGTTKRGVAFRFEPLNGPAVAFGKVPANDNGVARFTVRATAIGNYFAR
                     YAGDARNVPDEAQVHVITKARIVTRMGGHYGVSGAYRLFHTGAVPRYGVGVLPERRWS
                     VHFVLQRLRDGAWRTRAQTSFTTNR"
BASE COUNT          522 a         1306 c         1347 g          581 t
ORIGIN      
        1 ccagcgccca cgagcggagc ctaggcccgt tcgccccgga gcacgagctc ggcaccacga
       61 gctgaagcag gtcggctggg tggtctcgga cccgctccgg ctgcgagata cggcggagag
      121 ctgaccaccc tagagtgagc ccgtgaagct cgcggacgtg ttcgagggga tcttcgggac
      181 cgatctcccc gtgcggatcg agtgctacga cggcagcgcg accggggcgg aggactcgcc
      241 cgccacggtt gcgatcgcgt cgcctcgcgt cctccggcgg gtggccgccg ctccgggcga
      301 gctcggcttc gcacgcgcct acgtggccgg cgagctcgat gtccgaggcg atctcctgtt
      361 cgcgctttcc gccctccgcg agcgcgtccg ggacgtccgg ctgagcgccc gccaatgggc
      421 gacgatcgca cgcgtcgtcg gtcccgtggc gctccggccg ctcccgccgc cgcccgagga
      481 ggtccggctc cgcggtcgcc gtcactcgaa ggcgcgggac gccgccgcga tctcgcatca
      541 ctacgacgtg tcgaacgtgt tctaccggca catcctcggg ccctcgatga cgtactcgtg
      601 cgcggtctgg ccgagcgccg acgcgacctt ggagcgagcc caggaggaga agtacgagtt
      661 gatctgcctc aagctcgcgc tccgtcccgg gatgcggttg ctggacgtcg gctgcgggtg
      721 gggtgggatg gcgatgcacg cggccaccaa ccatggcgtc cgggccgtcg gcgtcacgat
      781 ctcgcggcat caggccgagt gggcggagaa ggcggtggcg gaggccggtc tctccgatca
      841 ggtggagatc cgcctccagg actatcgcga cgtccgtgac ggtccgtacg atgcgatctc
      901 ctcgatcggg atgttcgagc acgtcgggct ggcgcagctc ggcacgtact tcgaccggct
      961 ccacgcgctg ctccgcccgg agggtcggtt cctgaatcac gggatcagcc gtccgtcggc
     1021 ccggcgcgct cgcttcgccc ggcggagctt catcgatcgc tacgtcttcc ccgacggcga
     1081 gctgcacgag gtcggctcgg tcgtctcccg gatccagcgc tccggtttcg aggtccgcca
     1141 cgtggagggg ctccgagagc actacgcgct cacgctccgg gcgtgggtgg ggaacctgga
     1201 gcgctcgtgg gacgaggcgg tggccgaagc cggggtcgcc cgcgcccgga tctggcgtct
     1261 ctatatggcc ggggccgcgc tgaacttcga agcgggcgga cgcaggtcca ccaggtgctc
     1321 gcggtccgct ccgacgccgg ccggtccggg atgccgctcc gtcccgactg gtaggaggcg
     1381 gccgcgcccg ggtgcactgg gcttccgatc atgggggagg gacgatcggc gcgtcgtcgg
     1441 tgcaccccgg cgacgtctac gcgatctcga ctgccgaggg taacgtcgcc aagctcgagg
     1501 tcatccgttc ggctcggagc tcacgttccg gtacgtcacg tacggctgag gccccacctc
     1561 gacggcgagc gaccgcgagg gcagcttgta tcggaacgca accgaggtgt acggtcgccg
     1621 ccgggggtgg gccccatgcg agccgagcga cacaggagca tccggcggtc gctggtcgct
     1681 gtttcggcgc ttctcctcgt catcacactg accccttggg caggcgccga tgagctgtac
     1741 gagggtgacg tccccggtgg cgtcgacgtc cagctgctca ccgaccccgt ccacggtctc
     1801 gagctcacgg cgtcgacgga tgtctccgtt gcctcggccg ccaacccgga tctggagatc
     1861 ttcgatgcgg cgggcagctc cgtcgggacg tacgcgaccg atggcgttgg cggcctcgcg
     1921 atctccgacg acgggagcgt gctgttcgcg acgaagccca acgagccggc gatccggcgc
     1981 ctcgatctga cggacgttcc acccaccgag ctggcggacc ttgcgatcac cgatgtcttc
     2041 caaccgcact ccgtcgtctt cgccggcggc gcgctctggg tggcccagtg cctcgacgac
     2101 agcaccgtcg agaaggtcga ccccaccacg ggcgaggaga ccgcccagga catcaccggg
     2161 ctcgacatct cccgatgtcc ccagttcgtc gaccacccga cgctggcgaa cgtcttctac
     2221 gcctgggggc gattccaggg gacgctccag cgcttcgacg tgtcgtccgg gtcgctcgtg
     2281 gccacggcga gctggcccat ccccgggggc ggagcgatct ccgacgtcgc cgtcgcgccc
     2341 ggcggcggga cgctgatcgc cgcggtctcg ttcgcgtcgc cgtcgcccgg cgcggtggtg
     2401 ctggatccca ccaccctggt ggcgacgggt acgcgctacc gcgacggcac caacttgtcc
     2461 ggcgtgtcga tcgcggccga tggtcacgtg gccgtcgggt tcggaaccac ggttgaggtg
     2521 ttcgcttccg gtggtgcgtc gccgaccgcg acctggtcca gcgccgcgcc atgccgggtc
     2581 cgccagatca cctaccgcgg cgtcgacttc gggccgtcgg acgacgtgct gttcgtcaag
     2641 gaccaggagg gtccgagcgt cgcgatgctc tccaacccca cctcgaccct cgcgacatcc
     2701 acgatcagcg tctcggcgga gccgaacccc ggtgcgcccg gagaccccgt ggtcctgagc
     2761 ggggcgctgg acgtgggggg agcggccgac agcggccgca cggtcgagct cttccacagc
     2821 ggtgtgtcga tcgattcgac caccacgacg acgggcggcg ccttctcgtt cgacgtcgcg
     2881 ccgcccacgg ccggcgccca gtgcttcgac gtccgcttcg aggggatctc caccgcgacg
     2941 ggcgcctggt cctccgtgac cgtggacgtg ctgaagatcc cgtcgtccgt cgtgatcaac
     3001 ggcccgttga cggcgtatcc gagcgagtcg atcgagttct cggggacgct ggcgttcgac
     3061 gacgacgcat ccgccgaagg cgagatcgtc cacgtcggcc ggagtcagga cgggttcacg
     3121 ttcacgccgg ccggcgatgc ggtggtggag gcggacggga cctggagcat cacgacgaac
     3181 gcgccggcgg tcggcacctg gatctatcga gccgcctacg acggcaccga ccggtacgcg
     3241 aacgacctcg aaacgacgca gctgcaggtg gagcgccggc cgacgaacct gaccatcggg
     3301 gccagcaggg agcagctgat cttcggccag tcggtcacgc tgacggcccg gctcgacctc
     3361 ctcccgggga ccacgaagcg tggggtggcg ttccgcttcg aacccctgaa cgggccggcg
     3421 gtggcgttcg ggaaggtccc ggcgaacgac aacggggtgg cgcgcttcac ggtgcgggcg
     3481 acggcgatcg gcaactactt cgcgagatac gccggggatg cgcggaacgt gcccgacgag
     3541 gcgcaggtgc acgtcatcac gaaggcgagg atcgtgaccc ggatgggtgg gcactacggg
     3601 gtcagcggcg cctaccggct cttccacaca ggcgcggtcc ctcggtatgg ggtgggggtc
     3661 ttgcccgagc ggcggtggag cgtccacttc gtgctccagc ggctcaggga cggtgcgtgg
     3721 cggacgcgcg cgcagacctc gttcaccacg aaccgg
//
LOCUS       DASUVE010000372         5377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1125752, whole genome shotgun sequence.
ACCESSION   DASUVE010000372 DASUVE010000000
VERSION     DASUVE010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125752"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VF036_07990"
     CDS             <1..637
                     /locus_tag="VF036_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX6265206.1"
                     /translation="VDSRYPRVMDLAGFIAVATLVVVTPGPDMTLVARNTFAGGRPSG
                     LATSAGTCSGLLVHATAAALGLSAVLLASSQAFTVVKIAGAAYLIFLGIRTILGARRE
                     TSFRDGAKETGPWAAYRQGVLTNVLNPKVAIFFASLLPQFVQPGQGFTWRLLVLSGLF
                     ILMGSVWLTIYTIALHAVTGFVGLPRVKRIIETVTGSVLMALGVRLAFQRR"
     gene            complement(644..2497)
                     /locus_tag="VF036_07995"
     CDS             complement(644..2497)
                     /locus_tag="VF036_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX6265207.1"
                     /translation="MPAQAVELLSVTERSRYLSGLRVGLAAVVIATTLASVDVSSAQV
                     SVAVVLYLACSALVAVIVGRGGRTAGPTLNGSLLVDGIFLAIVIARTGGTAEALLLLP
                     GVHIVGVTLLLSYRTGLKIALWHTLLFLLAVEAARVGFLHGQVGAALGRGAGLVVAGL
                     WALALGTAFFSAVSERELRRQKHDLFRLSVMVAELDTDPDVHTIAQTFLDELCSTFGF
                     SRGVILASPGGDLEVLAWIGDGSPVDVSVGVDRTVSRAWAERTSQLLRSIDPEIDPRI
                     ASLIPDARNVVIVPLLRDRRQGLGAVIVERGDNHPTERRWVIEMVEQFAEHGALALHN
                     AWLTEERESQLRVIQGLEQQLRAHNASLEATVAKRTRELQGVVKELQEIGEQRKHLLD
                     HVVRAAEDERTRISHDIHDDPVQKMVALKMRLELLRRTHPEPAEMDEALAVMKVAIKS
                     MRTLLFDLSPPTLEEQGLASALRFMLEQSNWPFEWSVEEGADDDFPIGSSLILYRIAQ
                     EALTNARKHSGAECVSVNLERREGGTWMLIADDGVGFRPQDALVAAPGHLGLAAMKER
                     AEMAGGWCDLWSLPDEGTTLEVWLPDVETSHPAPSFGDGTVADPLPLPGRR"
     gene            complement(2524..3177)
                     /locus_tag="VF036_08000"
     CDS             complement(2524..3177)
                     /locus_tag="VF036_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265208.1"
                     /translation="MKWIGRSTLVVMSIIVIVATGAASAQAACPVLDPQCAIEDAVDD
                     GVGLVEDTVGGIETPVDDALDPLAEVVDPVVDTIFGTVDDVIGGGGREQPEPPGGAGD
                     DGGAQPGVHQGGPEPSVRPDRGTVDITGPRVYERPEIGRVFPPVVAPRPIADATGSPV
                     EGSGRVGAAIGAVARSLAIVLALFGLTLGFAAIQDRLDRNDPRLSLAPIRSDVVTFA"
     gene            3817..5058
                     /locus_tag="VF036_08005"
     CDS             3817..5058
                     /locus_tag="VF036_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6265209.1"
                     /translation="MSEARSAPETVEDPIRVLVADDEETVVDVLRTLIGSDPSLRFVG
                     AAHNAEDAIEMTARERPDVVLLDVRMPGGGGLRATREITKRCAPTKVVALSAHQDSDT
                     VIGMISAGASGYVPKGDPTEKILRTIHRAIDPDYVVEEAGPQLTLVSPMLPHRTQQRT
                     AVARAILDGVMTAEFEPIAELATGRVVGLDAHPRVATLPHRSYDAWLADAEGADLLLD
                     FELSAFRASLSALPIIPEDLFLGFEVTQSTVVEGRFRRAIRGSVAPRVVLGFSPLAPA
                     AAAELEAADPGGVLATLRARGIRVAARDAGPGFESLRHLSKLSPDLARLDPTLIRSLG
                     QTFSSHSVVAAVVACAGDVGARVIAPGVESEEQLHELRNLGVELVQGPIVGEPIPFSE
                     LHPEPGMWDAPRTWTPGDGAR"
     gene            5055..>5377
                     /locus_tag="VF036_08010"
     CDS             5055..>5377
                     /locus_tag="VF036_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265210.1"
                     /translation="MSRWSRRLSLIAVGTFSLALTGQPVANAACPVLYAACRAREALA
                     AGEDLVFETTDPVDTPVDGTVDPIVDDNLDRVRNLLGGGPIDLPDPIDDDPGGGHGGT
                     GPRPPG"
BASE COUNT          838 a         1885 c         1801 g          853 t
ORIGIN      
        1 cgttgactca cgctaccctc gcgtgatgga tctcgcgggg ttcatcgcgg tcgcgaccct
       61 cgtggtcgtg acacccggac ccgacatgac cctggtggcc cggaacacgt tcgcgggtgg
      121 ccgaccctcc ggactcgcga catcggcggg cacgtgctct ggcttgctcg tccatgcgac
      181 ggcggcggcc ctgggactgt ccgctgtgct cctcgcgtcg tcgcaggcgt tcaccgtcgt
      241 caagatcgcc ggcgccgcgt acctgatctt cctcgggatc cgcaccatcc tcggagcccg
      301 ccgagaaacg tccttccgag acggagcgaa ggagaccgga ccgtgggccg cctatcgaca
      361 aggggtcctc accaacgtcc tcaatcccaa ggtggcgatc ttcttcgcct cgctgctgcc
      421 gcagttcgtt caaccggggc agggcttcac ctggcgactg ttggtgctct ccgggctctt
      481 catcctgatg gggtcggtgt ggctcacgat ctacacgatc gcgctccacg cggtgaccgg
      541 gttcgtcggc ctcccccggg tcaagaggat catcgagacg gtcaccgggt ctgtactgat
      601 ggcactcggc gtccgactcg ccttccagcg acggtagctc ggctcagcgc ctgcccggca
      661 ggggcaaggg atcggcgacg gtcccgtcgc cgaacgatgg agcgggatga gatgtctcca
      721 cgtccgggag ccagacctcg agcgtggtcc cctcgtcggg gaggctccag agatcgcacc
      781 aaccccccgc catctccgcc cgctccttca tcgcggccag accgagatgg ccgggtgccg
      841 ccaccagggc atcctggggc ctgaacccga ccccgtcgtc ggcgatcagc atccacgtgc
      901 cgccctctcg ccgctccagg ttcacggaga cgcattccgc acccgagtgc ttgcgggcat
      961 tcgtcagtgc ctcctgcgcg atgcggtaca ggatgaggga gctcccgatc gggaagtcgt
     1021 catcggcgcc ctcctcgacc gaccactcga acggccagtt cgactgctcg agcatgaagc
     1081 gaagcgccga cgccaatccc tgctcctcga gcgtcggcgg gctgagatcg aacaggagcg
     1141 ttcgcatgct cttgatcgcc accttcatga cggcgagcgc ctcatccatc tccgccggct
     1201 ccgggtgggt cctgcggagc agctcgagcc gcatcttgag cgccaccatc ttctgcaccg
     1261 ggtcgtcgtg gatgtcgtgg gagatccggg tgcgctcgtc ctcggctgcc cgaaccacgt
     1321 ggtctagcag gtgcttccgt tgctcgccta tctcctgcag ttccttgacc acgccctgca
     1381 gctcgcgggt tcgcttcgcc acggtggctt cgagcgaggc gttgtgcgct cgcagctgct
     1441 gttcgagtcc ctggatgacg cggagctggg attcccgttc ctcggtgagc cacgcgttgt
     1501 gcaatgcgag cgcgccgtgt tcggcgaact gttccaccat ctcgatcacc cagcggcgct
     1561 ctgtgggatg gttatcgccg cgttccacga tcacggcgcc cagtccttgg cggcggtcac
     1621 ggaggagcgg gacgatcacc acgttccgcg catcggggat cagcgatgcg atgcgagggt
     1681 cgatctccgg gtcgatcgat cgcagcagct ggctcgtgcg ctccgcccac gcccgagaca
     1741 ccgttcgatc gacgccgacg gagacgtcga cgggcgagcc gtcgccgatc caggcgagga
     1801 cctcgaggtc gccgccaggc gacgcgagga tcacaccgcg cgagaaaccg aacgtggaac
     1861 agagctcgtc gaggaaggtc tgcgcgatgg tgtggacgtc ggggtcggta tccagctcgg
     1921 cgaccatcac ggagagcctg aagaggtcgt gcttctgtcg acggagctcc cgctcgctga
     1981 cggccgagaa gaacgccgtg ccgagcgcga gggcccagag gcccgcgacc acgagtcctg
     2041 cccctcggcc cagggcagcc cccacctgtc cgtggaggaa accgacccga gcggcttcga
     2101 cggcgaggag gaacagcaac gtgtgccaga gggcgatctt cagcccggtc cggtacgaga
     2161 gcagcagcgt gacgccgacg atgtgcaccc cgggcaggag gaggagcgcc tccgcggtcc
     2221 cgcccgtccg agcgatcacg atcgcaagga agatcccgtc caccagcaac gacccgttga
     2281 gggtcgggcc ggcagtgcga ccgccccgcc cgacgatcac cgcaaccagg gcggagcacg
     2341 ccaggtacag cacgacggcc acggagacct gtgcactcga cacgtcgacg gaggcgaggg
     2401 tggtcgcgat gaccacggcg gcgaggccga cacggagccc cgagaggtag cggctgcgct
     2461 ccgtgaccga tagcagctcc accgcctggg cgggcatcct ccgacgggta cgaggaaggg
     2521 cgatcacgcg aacgtcacca cgtcggatcg gatcggggcc agggagagcc gcggatcgtt
     2581 gcgatcgagc cgatcctgga tcgccgcgaa cccgagggtc aatccgaaca acgcgagcac
     2641 gatggccagg cttcgagcga cggcgccgat ggcggctccc acacgtccgc tcccctccac
     2701 gggagatcct gtcgcgtcgg cgatcgggcg cggtgccacg acgggtggga agacgcggcc
     2761 gatctccggc ctttcataga cccgcggacc cgtgatgtcc accgtccctc gatccggtcg
     2821 taccgagggt tccggaccgc cctgatgcac cccgggttga gctccaccat cgtcgccggc
     2881 gccccccggc ggctcgggct gctctctccc gccaccgccg atcacgtcgt caaccgtgcc
     2941 gaagatcgtg tcgacgaccg ggtccacgac ctcggcgagc ggatcgagcg cgtcgtccac
     3001 gggggtctcg atgcccccca ccgtgtcctc caccagaccc accccgtcgt ccacggcgtc
     3061 ctcgatcgcg cactgcgggt ccaggaccgg acacgctgcc tgcgccgacg cagcccccgt
     3121 cgcgacgatc acgatgatcg acatcacgac caaggtcgaa cgaccgatcc acttcacggt
     3181 gtcacctccg agcgtcgacc tgctgagatg gaacctgcct cgctcacggc gttcctttcg
     3241 gtctccctct ctgtatcggc tccaggacag cggtaaccca ataggcatcg gggttcatct
     3301 cctgtcatcc ccgttccgca tgggcgatcc acggcagtag ggcgccggag ggaaagcggg
     3361 ccccaaccgg ccgtgagggg aaacccgcag atgagctgtc ggcgtgctca cgggaaggaa
     3421 ggtcaggcct cggatacgtc cccgttctcg gtcgatcgac actcggtatc gatcggcagg
     3481 cttcaaccga gtttcacgtt cgtttgtcgg aaccacgcaa cggatgagag gaagggatcc
     3541 gacggcacga tcgagggcgg ggacgcgcga tcggttgtca tcgcgggcgc cgccgcgccc
     3601 gaagatggtc tttcgccccc cgaccgatgc gcgtctatcg gccgggcgag ggagggttga
     3661 gtgcgcagcc cacctggtca gggcgaatca cccgagtttg acgcgaagga accatcgtcc
     3721 caggtcacgc gtagaccgat acctcgttcc aggtagacgt gggagctccg gatccggcct
     3781 ctgaagcctc cggagctccg gatggaagga gcgggagtga gcgaagcgcg gagtgccccg
     3841 gagacggtcg aggatccgat ccgtgtcctg gtcgccgatg acgaggagac ggtcgtcgac
     3901 gtcctccgca cgctcatcgg atcggatccg tcccttcgct tcgtgggagc ggcccacaac
     3961 gccgaagacg cgatcgagat gacggcgcgg gaacgcccgg acgtggtcct actcgacgtg
     4021 cggatgccgg gcggtggagg actgcgggcg acccgcgaga tcacgaagcg atgcgcgccg
     4081 acgaaggtcg tcgcgctctc ggcgcaccag gattccgaca ccgtgatcgg catgatcagc
     4141 gccggggcga gcggctacgt tcccaagggc gatccgacgg agaagatcct gcggacgatc
     4201 caccgtgcga tcgatcccga ctacgtcgtg gaggaggcgg gaccgcagct cacgctggtc
     4261 tcgccgatgc ttcctcatcg cacgcagcag aggaccgccg tcgcccgagc gatcctcgat
     4321 ggggtcatga cggccgagtt cgagccgatc gcggagctcg ccactggcag ggtcgtcggc
     4381 ctcgatgccc atccccgcgt ggcgaccctc ccccaccgtt cgtacgacgc ctggctggcc
     4441 gacgccgagg gtgccgacct cctcctcgac ttcgagctgt ccgcgttccg agccagtctg
     4501 tcggccttgc cgatcatccc cgaggacctg ttcctgggat tcgaggtgac gcagtcgacc
     4561 gtggtggagg gaaggttccg ccgagcgatc cgagggtcgg tcgccccgcg ggtcgtgctg
     4621 gggttctcgc ctctcgcgcc cgcggccgcg gccgagctcg aggccgcgga tcccggcggg
     4681 gtcttggcca cgcttcgcgc gcgggggatc cgcgtcgcgg cgagggacgc aggccccggg
     4741 ttcgagagcc ttcggcacct ctccaagctc tcccccgacc tggccaggct tgatccgacc
     4801 ctcatccgga gtctgggaca gaccttctcg agccattccg tcgtcgccgc ggtggtcgcc
     4861 tgcgccggtg acgtcggggc ccgcgtgatc gcgcccggcg tcgagtccga ggagcagctc
     4921 cacgagttgc ggaacctcgg tgtcgagctc gtccaaggac cgatcgtcgg ggagccgatc
     4981 ccgttctcgg agcttcatcc cgagcccggg atgtgggatg ccccgcggac ctggaccccg
     5041 ggcgatggtg cccggtgagt cgctggagcc ggcgcctctc gctgatcgcg gtcgggacct
     5101 tctccctcgc cctcaccggc cagccggtcg cgaacgcggc ctgtcccgtg ctgtacgccg
     5161 cctgccgcgc ccgcgaggct ctcgccgccg gtgaggatct cgtgttcgaa acgaccgacc
     5221 cggtcgacac tcccgtcgac ggcacggtcg acccgatcgt ggacgacaac ctggatcggg
     5281 tccgcaacct cctcggcggc ggtccgatcg acctcccgga cccgatcgat gatgatcccg
     5341 gcggtggcca cggcgggacg ggaccccggc ctccggg
//
LOCUS       DASUVE010000373         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_563178, whole genome shotgun sequence.
ACCESSION   DASUVE010000373 DASUVE010000000
VERSION     DASUVE010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563178"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VF036_08015"
     CDS             <1..350
                     /locus_tag="VF036_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6265211.1"
                     /translation="GLGVEVDANGLGRFPADIETTVYFCALEALQNVAKYADAAHTVV
                     HLTRSNGDLSFEVVDDGRGFDPNGVAGGSGLQGMADRLAAVGGSLEIRSAPGRGTTVA
                     GHVPIHGDCGGSP"
     gene            347..1105
                     /locus_tag="VF036_08020"
     CDS             347..1105
                     /locus_tag="VF036_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265212.1"
                     /translation="MMETRSTSSSLAFRIVGWVLGVLTIGYSFFFIVDSILSGDPGAQ
                     SHRFHNLGGFAGGGLIGVFSILFVLRPDWTAVWHVLVAQALAWTFGGLMGGDLVSGLL
                     FIAPLGMVLLAILHPAPRSLLRLPGRPSVALLTYALLGTIPAWIYAVINAELQHGPAT
                     DPHVEFHHWSGVAVSALAIGGAALATSLRGVGWQVTGVVTALAAVLFGVAGLVFADYA
                     GAPDDTAWSWLAIAAGIGFWILVRIENAREASAG"
     gene            1102..1272
                     /locus_tag="VF036_08025"
     CDS             1102..1272
                     /locus_tag="VF036_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265213.1"
                     /translation="MTSKSRSPLRRLERWLVGIFMAALVFVLERLVMRQIRKKEAARD
                     ASTAGAVTVGES"
     gene            1275..2078
                     /locus_tag="VF036_08030"
     CDS             1275..2078
                     /locus_tag="VF036_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6265214.1"
                     /translation="MHRWWVFLHIAGAFGFLMAHGVSAYVTLRLPKERDPARVSQLLE
                     LSASSVGFMWNSIGLLLLGGIAAGFTGHFWGQGWIWAAIVILVVVIAAMYAMGTTWAK
                     RLRTISGAMVEGTEAVSTPQFEEILRSKRPYTIAAIGFVGLLAILWLMIFKPTLGFGG
                     AEAAAGECTLGTGSAVTVCAPDDQRFVPERLEAPAGQPFVMRFLNEDANIEHNVAIYT
                     DESASESLFVGDLILGIGDGRYEVPALDPGTYYFRCDLHPQMDGTLEVG"
     gene            2079..>2312
                     /locus_tag="VF036_08035"
     CDS             2079..>2312
                     /locus_tag="VF036_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6265215.1"
                     /translation="MPYRTIVVGTDGSPTATVAVEVAQKLTKRLRGRLVLVGALDAYG
                     VSRQPLQTALYEAAEGARAKKVDATAELIEGTPG"
BASE COUNT          314 a          759 c          827 g          412 t
ORIGIN      
        1 gcgggctcgg tgtggaggtc gacgcgaacg ggctcggacg gttccctgcc gacatcgaga
       61 ccaccgtgta cttctgtgcc ctcgaggcgc tccagaacgt cgcgaagtac gcggacgccg
      121 cgcacacggt cgtccatctg acccgctcga acggcgacct ctcgttcgag gtcgtggacg
      181 acggccgggg cttcgacccc aacggcgtcg cgggcggctc tgggctgcag ggcatggccg
      241 accggctcgc ggccgtggga ggctctctcg agatcagatc ggcgccgggc cgcggcacga
      301 ccgtcgccgg tcacgtgccg atccacggag attgcggagg cagcccgtga tggagaccag
      361 atcgacgagc agctcactcg cgttccggat cgtcggctgg gtccttgggg tgctcacgat
      421 cggctactcc ttcttcttca tcgtcgattc gatcctgtcc ggcgatccgg gcgcccagag
      481 ccaccggttc cacaacctgg gcgggttcgc cggcggcggt ctgatcgggg tgttctcgat
      541 cctgttcgtg ctgcggcccg actggacggc ggtctggcac gtcctggtcg cgcaggcgct
      601 cgcctggacg ttcggtggac tgatgggcgg ggacctcgtg tcgggattgc tcttcatcgc
      661 gcccctcggg atggtgctcc tcgcgatcct gcaccccgcc ccccggtcgc tcctccgtct
      721 cccgggtcgg cccagcgtgg cgctgctcac gtacgcgctg ctcgggacga tcccggcctg
      781 gatctacgcg gtcatcaacg cggagctcca gcacgggccc gcgacagatc cccacgtcga
      841 gttccatcac tggtccgggg tggccgtctc ggccctggcc atcgggggtg cagcgctcgc
      901 gacgtcgctc cgcggcgtcg ggtggcaggt gacgggggtc gtgacggcgc tcgcggccgt
      961 tctgttcggc gtcgccgggc tcgtgttcgc ggactacgcg ggagcccccg acgacaccgc
     1021 ctggtcgtgg ctcgcgatcg ccgcagggat agggttctgg atcctcgttc ggatcgagaa
     1081 cgcgcgagag gcgtccgccg ggtgacgtcg aagtctcggt ccccactccg gcggctcgag
     1141 cggtggctcg tcggcatctt catggccgcg ctggtgttcg tcctggagcg gctcgtgatg
     1201 cggcagatcc ggaagaagga ggccgctcgc gacgcatcga cggccggcgc cgtcacggta
     1261 ggggagagct agcgatgcat cgttggtggg tcttcctcca catcgccggg gcgttcggtt
     1321 tcctgatggc gcacggcgtc tcggcctacg tgacgcttcg gctcccgaag gagcgtgacc
     1381 cggcccgcgt gagccagctg ctcgagctct cggcgtcctc ggtgggcttc atgtggaact
     1441 cgatcggcct cctcctcctc ggcgggatcg ctgccgggtt caccgggcac ttctggggtc
     1501 aggggtggat ctgggcggcg atcgtgatcc tggtcgtcgt gattgcggcg atgtacgcca
     1561 tgggcaccac gtgggcgaaa cggctgcgga cgatctccgg cgcgatggtc gagggaacgg
     1621 aggccgtctc gacgccgcag ttcgaggaga tcctccgatc gaagcgtccc tacacgatcg
     1681 cggcgatcgg gttcgtcggc ttgctcgcga tcctgtggct gatgatcttc aagccgacgc
     1741 tcggcttcgg cggggcagag gctgcggccg gggagtgcac gctcgggacg gggagcgccg
     1801 tgaccgtctg tgccccggac gatcaacgct tcgtgccgga gcgtctggaa gcgcccgccg
     1861 gtcagccgtt cgtgatgcgc ttcctgaacg aggatgccaa catcgagcac aatgtcgcga
     1921 tctacaccga cgagtcggcg tcggagtcac tcttcgtcgg ggatctgatc ctcgggatcg
     1981 gagacggcag atacgaggta ccggccctcg atcccggtac ctactacttc cgctgcgacc
     2041 ttcatccgca gatggacgga accctggagg tcgggtagat gccctaccgg acgatcgtcg
     2101 tgggcaccga cggctcgccg accgcgacgg tcgccgtgga ggtggcgcag aagctcacga
     2161 aacggctccg cggacgtctg gtcctcgtgg gagcgctcga tgcatacggc gtgtcacgcc
     2221 agcctctgca gacggctctc tacgaagcag cggagggcgc acgagcgaag aaggtcgatg
     2281 ccaccgccga gttgatcgaa ggcaccccgg gg
//
LOCUS       DASUVE010000374         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_937295, whole genome shotgun sequence.
ACCESSION   DASUVE010000374 DASUVE010000000
VERSION     DASUVE010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937295"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VF036_08040"
     CDS             <1..838
                     /locus_tag="VF036_08040"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6265216.1"
                     /translation="LGAMGARFAGRFLEAGHEVIVWNRTLEKAEELVSRGASAAATPA
                     EAARNAEGVVTAVSDPEALRAVTDGPDGIAAGSNASTTVIEMSTVGPRAVRWLETALP
                     PGVGLLDAPVLGSLSEAEAGTLLVFVGGPRRLAERWIPLFGTLGSVIVAGPLGSGAAA
                     KLVANATLVGTLTLLGETIALADRLGLGRRLTMDILAQTPLGLASKRRREQLETEDFP
                     LRFRLALARKDADLIQEAAEAAHADTRVLAAAATWFVDAEEAGLGDEDYSTVLQRIID
                     HG"
     gene            890..965
                     /locus_tag="VF036_08045"
     tRNA            890..965
                     /locus_tag="VF036_08045"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:924..926,aa:Glu,seq:ctc)
     gene            1019..1501
                     /locus_tag="VF036_08050"
     CDS             1019..1501
                     /locus_tag="VF036_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6265217.1"
                     /translation="MSGDRSYIEENTRERERMRAIVERVSDNELCMSVNPHWNVAAVL
                     GHIAFWDARVLSLADKLERGEPFTTSDTEPEDVDWINDASRALIHAIPPREAARLALE
                     IAEETDARVATLPVDRLWPTDPDSPLYALRASHRGEHLDEVEAALRDPSGPSGDAPTI
                     "
     gene            complement(1498..>2099)
                     /locus_tag="VF036_08055"
     CDS             complement(1498..>2099)
                     /locus_tag="VF036_08055"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX6265218.1"
                     /translation="GNTWSMRGTVVVACNCDYGCPCNFQALPTMGKCEGGWTWHVEEG
                     SYGNVRLDGITFTLTVNWPGPIHEGNGVALLLVDERTDEAQRDAIHTLAKGEVGGPWG
                     VLGWTWPTLEGPEAVPFDLHLDGIRSSVRAGDAYELESEPIRNPVTGAEISPGVMLPQ
                     GTIFKEGLFGSSKRLRVDRLDIDYANKYTAVAAFEYRGP"
BASE COUNT          333 a          733 c          717 g          316 t
ORIGIN      
        1 tctcggagcg atgggcgccc ggttcgcggg caggttcctc gaagccggcc acgaggtgat
       61 cgtctggaac cggacactcg agaaggccga agagctcgtc tcgcgagggg cgtcggcggc
      121 ggccacaccc gccgaggctg cgcggaacgc cgaaggagtc gtcacggccg tgtcggaccc
      181 cgaggcgctg cgagcggtca ccgacggccc ggacggcatc gccgccggct cgaacgcctc
      241 gaccacggtg atcgagatgt cgacggtcgg accccgggcg gtgcgctggc tggagaccgc
      301 gctgccgccg ggggtgggac tgctggacgc tcctgtcctc ggcagcctgt ccgaagcgga
      361 ggcgggcacg ctcctcgtgt tcgtcggagg cccgcgccgt ctcgccgaac ggtggatccc
      421 gctcttcggg acgctcggct cggtgatcgt tgccggtccc ctcgggtccg gagcggcggc
      481 caagctggtg gcgaacgcca cgctggtcgg cacgctgacc ctcctgggcg agacgatcgc
      541 cctcgcggac cgactcggcc tcggacggag gctcaccatg gacatcctgg cgcagacgcc
      601 gctcggcctg gcctcgaaga ggcggcgcga acagcttgag accgaggact tcccactgcg
      661 attccgcctc gccctggctc ggaaggacgc cgacctgatc caggaggcgg cggaggcggc
      721 gcacgcggac acgcgggtgc tcgcggcggc cgccacgtgg ttcgtcgatg ccgaggaagc
      781 cgggctcggc gacgaagact actcgacggt cctccagcgg atcatcgacc acggctgatc
      841 gtttcccgac cggtgttatc ctcgctgact gggtccatcg agcggccgcg gtcccatcgt
      901 ctagaggcct aggacatcac cctctcaagg tgaagacacg ggttcaaatc ccgttgggac
      961 taccagcgtc ctggcggcag gaaccgacat cgctcccgcg cgctatggtc atggagccat
     1021 gtcgggggat cggtcgtaca tcgaggagaa cacgcgagag cgcgagcgga tgcgcgcgat
     1081 cgtcgagcgc gtgagcgata acgagctctg catgtccgtc aaccctcact ggaacgtcgc
     1141 cgccgtcctc ggccacatcg cgttctggga cgcccgggtg ctctcgctcg ccgacaagct
     1201 cgagcgggga gagccgttca cgacctccga cacggagccc gaagacgtcg actggatcaa
     1261 cgacgccagc cgtgcgctga tccacgcgat cccgccgcgg gaggccgctc gcctcgccct
     1321 cgagatcgcg gaggagaccg acgcacgggt ggcgaccctc cccgtcgacc gtctctggcc
     1381 gaccgacccg gacagcccgc tgtacgcgct gcgcgcgtcg caccgaggcg agcatctcga
     1441 cgaggtcgaa gcggcactac gggatccgtc cggtccgagc ggtgacgccc cgacgattta
     1501 gggacctcgg tactcgaagg ccgcgacggc cgtgtacttg ttcgcgtagt cgatgtcgag
     1561 ccgatcgacc cgcagccgtt tggacgagcc gaagagacct tccttgaaga tcgtcccctg
     1621 gggcagcatc acgcccggag agatctcggc gccggtcacc gggttccgga tcggctcgga
     1681 ctcgagctcg taggcgtcgc cggcgcggac gctcgaccgg atgccgtcga ggtggagatc
     1741 gaacgggacc gcctccggac cctcgagcgt cggccaggtc cacccgagga cgccccacgg
     1801 tcctccgacc tcacccttgg cgagggtatg gatcgcgtcc cgctgcgcct cgtccgtgcg
     1861 ttcgtccaca aggaggagag caaccccgtt gccttcgtgg atcggtccgg gccagttcac
     1921 cgtcagcgtg aacgtgatgc cgtcgagacg aacgtttcca taggaaccct cctccacatg
     1981 ccaggtccat ccaccctcgc acttgcccat cgtggggagg gcctggaagt tgcacgggca
     2041 cccgtagtcg cagttgcacg cgacgacgac cgttcccctc atcgaccacg tgttgccca
//
LOCUS       DASUVE010000375         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_751646, whole genome shotgun sequence.
ACCESSION   DASUVE010000375 DASUVE010000000
VERSION     DASUVE010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751646"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VF036_08060"
     CDS             complement(<1..754)
                     /locus_tag="VF036_08060"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX6265219.1"
                     /translation="MRRLLAIFATTLLFTLAPTPASAIVNGQVDEDNDFPFVGMLAFH
                     DADGDYLHRCTGTLISPTVVLTAAHCTVGTAKAYAYFDVEVVADFRENPSGIKGKPYT
                     SPDFNPNTLSNDVGVVVLKTAVQLREYPTVVEEGFLSEQKAHNGLKDATFTVVGYGGT
                     AGSPPPNLMFDLFRRYALAPYGGLTQNNLHLQANPNATGLGGTCFGDSGGPILWDETL
                     TIVAVTSWGDAICRSNDMTQRLDLASVQDFLSR"
     gene            complement(898..1581)
                     /locus_tag="VF036_08065"
     CDS             complement(898..1581)
                     /locus_tag="VF036_08065"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEX6265220.1"
                     /translation="MKRIIGLALALTTTMALGANTPAAASESEEFSLNVVHGIPGVTV
                     DVCVNGAKAITDFQPGDVVSDVRLQEGEYRLKVTPAGEPCTAAILQARAQLEGGRYRS
                     YTVVANLDDEGAPNLLLFRNPMRVTDAGSARLVIRHTADAPAVTVWADGSKLNRGRGF
                     EWGDSRRYSVPAETYSVAVSPAGSTTPVIGPTDLTVRAGFSYQVYAWGNGDAGYALAI
                     VATQVGQAH"
     gene            complement(1767..>2185)
                     /locus_tag="VF036_08070"
     CDS             complement(1767..>2185)
                     /locus_tag="VF036_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265221.1"
                     /translation="SAMAPPTSRPSPVVLACGPKDLHTIGLEAFAVVLTRRGWPCRVL
                     GALTPTGSLVGAVDTLKAPAGVVVAQRGVTRRAAIDSIAAVDALPRVRAFYAGDAFAA
                     PTARRDVPGVYLGTDVIAAAEILESELTRLGFAQFA"
BASE COUNT          356 a          735 c          735 g          359 t
ORIGIN      
        1 acctcgatag gaaatcctgc accgaggcga ggtcgagccg ctgggtcatg tcgttcgacc
       61 gacagatcgc atcgccccag ctcgtgacgg cgacgatcgt gagcgtctcg tcccagagga
      121 tgggaccacc ggagtcgccg aagcacgttc cgcccaggcc cgtggcgttc gggttggcct
      181 ggaggtgcag gttgttctgc gtgagcccgc cgtacggcgc gagggcgtag cgacggaaca
      241 ggtcgaacat gaggttcggc ggcggtgaac ccgccgtgcc cccgtatccg acgacggtga
      301 acgtcgcgtc cttcagcccg ttgtgcgcct tctgctcgct gaggaagccc tcctcgacga
      361 ccgtcgggta ctcgcgcagc tgaacggccg tcttcaggac gacgaccccc acgtcgttgc
      421 tgagcgtgtt gggattgaag tctgggctcg tgtatggctt gcccttgata ccgctcgggt
      481 tctctcggaa gtctgcgacg acctccacgt cgaagtacgc gtacgccttg gcggtgccga
      541 ccgtgcagtg ggccgccgtc aggaccaccg tcggcgagat cagagtaccg gtgcaccgat
      601 gcagatagtc accgtcggcg tcgtggaagg cgagcatccc gacgaacggg aagtcgttgt
      661 cctcgtccac ctgtccattg acgatggcgc tggcgggtgt cggcgccagg gtgaacagca
      721 gcgtcgtagc gaagatcgcg agcaaccgcc tcatcgaggc ctcctgtggg gggtcgggga
      781 ccgtcaccgt gactgagccc acgagcaccc aacaacagcc gcatcgacgc aggctgaaat
      841 gcaaagggcg gcggccggag ccgccgccct ccacggtgag gagccgtgac gatggggtca
      901 gtgcgcctga ccgacctgcg tcgccacgat cgccagcgcg tacccggcgt caccgttccc
      961 ccaggcgtac acctggtagc tgaacccggc ccgaaccgtc agatcggtgg gaccgatcac
     1021 cggggtggtc gagcctgcgg gcgagacggc caccgagtac gtctcggcgg gaacggagta
     1081 acgacgcgag tcgccccact cgaacccacg gccccggttc agcttcgagc cgtccgccca
     1141 cacggtcact gcgggggcat cggccgtgtg ccggatcacg agacgggcgg agccggcgtc
     1201 cgtcacgcgc atcgggttcc ggaagagcaa caggttcggc gctccttcgt cgtcgaggtt
     1261 cgcgaccacc gtgtagctgc gatagcgtcc tccttcgagc tgggctcgcg cctgcaggat
     1321 cgccgcggtg cacggctcgc ccgccggtgt gaccttcagg cgatactcgc cctcctgcag
     1381 ccgcacgtcg ctcacgacgt cacccggctg gaaatctgtg atcgccttcg cgccgttcac
     1441 gcacacgtcg acggttacac ccgggatccc gtgcacgacg ttcaacgaga actcctcgct
     1501 ctccgacgcg gcagcgggcg tgttggcgcc cagggccatc gtcgtggtca gcgcgagggc
     1561 cagaccgatg atccgcttca ttcgtttcct ccctttcctc gatccttccg gtggttccaa
     1621 gcgatccgtc tcgcctccat cgatggcatc acctctccct gtggtggacc tggatccatc
     1681 attcacgagg agggcgaggg aagccatgca tgaacctatg cacttgtctt tcggaggcca
     1741 tcagatgggc agtgccgctc ttcgggtcag gcgaactggg cgaagccgag ccgcgtcagc
     1801 tcggactcga ggatctcggc ggcggcgatg acatctgttc cgaggtagac acccggcacg
     1861 tccctgcgag ccgtcggggc ggcgaaggca tcccccgcgt agaacgcccg aacacggggg
     1921 agtgcatcca cggcggcgat cgagtcgatc gcggcccggc gcgtgacgcc gcgttgggcg
     1981 accaccacgc cggccggcgc tttcagggta tccaccgcac cgaccaggga tccggtcggg
     2041 gtcagtgccc ccaggacgcg gcacggccat ccgcgtcgcg tgaggacgac tgcgaacgcc
     2101 tcgagcccga tcgtgtggag atccttcgga ccgcaggcga ggacgaccgg gctgggacgc
     2161 gatgtcggag gtgccatcgc cgacc
//
LOCUS       DASUVE010000376         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1316556, whole genome shotgun sequence.
ACCESSION   DASUVE010000376 DASUVE010000000
VERSION     DASUVE010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316556"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VF036_08075"
     CDS             complement(<1..636)
                     /locus_tag="VF036_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265222.1"
                     /translation="MTPPPPPLPPAANVSPRPDASPRTGRPFRRWLPTGLVALVMSVT
                     VLGGFVVAGTLPVPNVRSVALGGVVTVHPLPGWEVAHREQVSLPSASGGIIPGEFAQL
                     TRGNGALDLLALPDVGGGALALADLYAGEVLSHQLERLSVTHRVEQILLRSGLEAVRF
                     GYIGSEPQSGSPVEGSVIVTVSPAGNGVIFDGWAFQGQLELIVEELTAMIDE"
     gene            complement(636..1055)
                     /locus_tag="VF036_08080"
     CDS             complement(636..1055)
                     /locus_tag="VF036_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265223.1"
                     /translation="MTITVVGGEPSGLASMVADLIEQNLARDPTRSALLRRSVSVLDA
                     HDVAVMVFLRIERDGVRVGDGDVPDAHLRIRSDSGRLLDLTTAPLRGGLPDPLRPEGR
                     AIVGDLIRRRIRIRGLLRHPLRLVRLTKLLSVADGDR"
     gene            complement(1052..2092)
                     /locus_tag="VF036_08085"
     CDS             complement(1052..2092)
                     /locus_tag="VF036_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6265224.1"
                     /translation="MRGVVFDEVGAVRVADLPDPDLEAPTDAIVRVTRSAICGSDLHL
                     LHGKAPMEPGEPLGHEAVGVIEAVGAGVETRRVGDRVAVAFNIACGHCWFCGIGQSSL
                     CDDDRIFGYGIFGGALPGAQAERLRVPDADLHLLRIPDAVRDEAAVFVGDVLTTGFYG
                     ASLSGAGPDEVVAVLGCGPVGFCTIEGLRAQGGPAIYALDRDPSRLVLAETAGAIPVH
                     IDERNPVIALAEATDGRGADVVIDAVGHPTAFEGAIDTVRRGGTVVVLGVYSSETTEL
                     QLGAYWSRALTLRFAGLTPVVSWWGKAMDGLERGAVDPTPLISHRLPLEAAAEGYALF
                     DRREATKVVLEP"
     gene            complement(2092..2511)
                     /locus_tag="VF036_08090"
     CDS             complement(2092..2511)
                     /locus_tag="VF036_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX6265225.1"
                     /translation="MPEFPTEEWFQAYIQAVDASDEYAEYAATWEGDAVIHVEAEPDK
                     GIPTDVRGLLDLWHGGCRGGGIVDEVRAASAEFVIRAPYSRWKDVIRGNLEPVKGLVQ
                     GKLRVRGDLPKILRYTKGTQELAYIAGQVDTTFPDEP"
BASE COUNT          424 a          974 c          888 g          356 t
ORIGIN      
        1 ctcgtcgatc atcgccgtga gctcctcgac gatcagctcg agctgcccct ggaacgccca
       61 gccgtcgaag atcaccccgt tgccggcggg cgagaccgtg acgatgacgg acccttccac
      121 cggcgatccg ctctgcggct ccgacccgat gtagccgaag cggacggcct cgagcccgct
      181 ccggaggagg atctgctcca cccggtgcgt cacggacaac cgctcgagct ggtggctgag
      241 cacctcaccg gcgtagaggt ccgccagcgc gagcgcccca ccgcccacat cggggagcgc
      301 cagcaggtcg agcgccccgt tgccgcgggt gagttgcgcg aactcccccg ggatgatccc
      361 gccggacgcc gaggggagag agacctgctc gcgatgcgcg acctcccacc ccgggagcgg
      421 gtggaccgtg acgacacctc cgagagcgac cgaccggacg ttcggcacgg gcagcgtccc
      481 cgccaccacg aacccaccga gcacggtcac ggacatcacg agcgcgacga gaccggtggg
      541 gagccaccgc cggaagggcc ggccggtcct gggggaggcg tccggccggg gcgagacgtt
      601 cgccgccggc ggcagcggcg gtggtggtgg tgtcatcacc gatccccgtc cgcaacggac
      661 aggagcttcg tgagtcgcac caggcgcagc gggtgccgga gcaaccctcg gatccggata
      721 cgccggcgga tgagatcccc gacgatcgct cggccctccg gccggagcgg gtccggaagc
      781 cctcctcgca gcggcgcggt ggtgaggtcg agcagccgcc ccgagtccga ccggatccgg
      841 aggtgcgcat cgggaacgtc gccgtcgccg acccggacac catcgcgctc gatccggagg
      901 aagaccatca cggcgacgtc gtgcgcgtcg agcaccgaga ccgaccttcg caggagcgcg
      961 ctgcgtgtgg gatcgcgcgc gaggttctgc tcgatgaggt ccgcgaccat cgacgcaagg
     1021 ccgctcggct cgccgccgac caccgtgatc gtcacggctc gaggaccacc ttcgtggctt
     1081 cccgccggtc gaagagggcg taaccctcgg cggctgcctc gagcgggagt cgatggctga
     1141 tcagcggcgt cgggtccacc gcgccacgct ccaggccgtc catcgccttg ccccaccacg
     1201 agaccacggg cgtgagcccc gcgaaccgga gcgtgagcgc tcgtgaccag tacgccccga
     1261 gctggagctc ggtcgtctcc gacgagtaga cgccgaggac gaccaccgtg ccgccccgtc
     1321 ggacggtgtc gatcgcgccc tcgaaggccg tcggatgtcc gaccgcgtcg atcaccacgt
     1381 cggctcccct tccgtcggtc gcctccgcga gcgcgatcac ggggttccgc tcgtcgatgt
     1441 gcaccgggat cgcgcccgcc gtctccgcta acaccagccg cgacggatct cgatcgagcg
     1501 cgtagatcgc cggaccacct tgggctcgga ggccctcgat cgtgcagaac ccgaccgggc
     1561 cgcaaccgag caccgcgacc acctcatccg ggccggcgcc cgacagcgaa gccccgtaga
     1621 agccggtggt cagcacatct ccgacgaaga cggcggcctc gtcccgcacc gcgtccggga
     1681 tcctcagcag gtgcagatcg gcgtcgggga cgcggagacg ctcggcctgg gcgccgggca
     1741 gggcaccgcc gaagatcccg tagccgaaga tcctgtcgtc atcgcacagt gaggactgcc
     1801 cgatcccaca gaaccagcaa tggccgcagg cgatgttgaa cgcgaccgcg acacggtctc
     1861 cgacccgccg ggtctccacc ccggcgccga ccgcctcgat cactccgacg gcctcgtgcc
     1921 ccaacggctc ccccggttcc atcggcgcct tcccgtggag gaggtggaga tcgctcccac
     1981 agatcgccga ccgtgtgaca cggacgatcg catcggtcgg cgcctcgaga tcgggatcgg
     2041 ggaggtcggc gacacgcacg gcgcccacct cgtcgaacac gaccccccgc atcagggttc
     2101 gtcggggaag gtggtgtcca cctgcccggc gatgtaggcc agttcctggg tgcccttcgt
     2161 gtagcgcagg atcttcggga ggtcgcctcg gacacggagc ttcccctgga cgagcccttt
     2221 cacgggttcg aggttcccgc ggatcacgtc cttccatcgc gagtacggcg cgcggatcac
     2281 gaactcggcg gaggccgccc tcacttcgtc gacgatgccg ccgcctcggc agccgccgtg
     2341 ccagaggtcg aggaggccac gcacgtcggt cgggatcccc ttgtcgggtt ccgcctcgac
     2401 gtggatcacc gcgtcgccct cccaggtcgc cgcgtactcg gcgtactcgt ccgacgcatc
     2461 gaccgcctgg atgtacgcct ggaaccactc ctccgtcggg aactcgggca tgtcaggacc
     2521 cccacagctg caaggcgaag aggaccccga ccgcattgaa cgcagcatgg gtcgcgatgt
     2581 tcacgaggag gttgcgatgg cgccagtgga tccaggccca gaccaggccg atgatcatca
     2641 tc
//
LOCUS       DASUVE010000377         2773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_184670, whole genome shotgun sequence.
ACCESSION   DASUVE010000377 DASUVE010000000
VERSION     DASUVE010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184670"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VF036_08095"
     CDS             <1..196
                     /locus_tag="VF036_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010902321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265226.1"
                     /translation="KGKGVPHLQRRGRGDLFVTLHIVTPRDLSKEERTLLQRFAEIRD
                     AGAGGNGAAPRALRRPEYRS"
     gene            234..587
                     /locus_tag="VF036_08100"
     CDS             234..587
                     /locus_tag="VF036_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX6265227.1"
                     /translation="MQTDSSCLFCRIAAREIPADIVRESDRLVAFRDTNPQASTHILL
                     IPKEHVRSIAEIEDQHADLLADIAQAATQLAAAEGIEESGWRLVTNVGPDAGQSVFHL
                     HFHLLGGRQMGWPPG"
     gene            724..1683
                     /locus_tag="VF036_08105"
     CDS             724..1683
                     /locus_tag="VF036_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX6265228.1"
                     /translation="MVALLGQQDAFLKLIEAGFSSDILVRGNEITITGPEHEAEQLSH
                     LFEELLALLEKGHHLSDSSVGQTIELIKGGDEWVEGEDGTARMRPSEVLGDTLLTARG
                     TSVVPKTVGQKRFVDAMRRATVTFAIGPAGTGKTYLAVATAVRALQDHQVSRLILTRP
                     AVEAGERLGFLPGTLYEKIDPYMRPLYDALFEMTGAESLSRLMERGTVEVAPLAYMRG
                     RTLNDAFIILDEAQNTTPEQMKMFLTRIGFGSKAVVNGDDTQVDLATGQRSGLLVIEE
                     ILSGIRGISFCRLGGRDVVRHKIVQEIVEAYDRYGQQRLGMDD"
     gene            1683..2180
                     /gene="ybeY"
                     /locus_tag="VF036_08110"
     CDS             1683..2180
                     /gene="ybeY"
                     /locus_tag="VF036_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX6265229.1"
                     /translation="MADDDHPVDPGPADGAWPRVLISNRQRRELDVEGLRSLARDTLR
                     GEGVDRAELSVSFVETGEIEELHERFMDEPGPTDVLSFPLVEEAEGPAKDGAKDGDGT
                     RHLGDVVVAPAEAERNNPADPDGEIRLLLVHGILHLLGYDHEDEDARTRMWERQERYS
                     GVRAP"
     gene            2177..>2773
                     /locus_tag="VF036_08115"
     CDS             2177..>2773
                     /locus_tag="VF036_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HEX6265230.1"
                     /translation="MSSLWLVALLLVLFGTVLAAAEASLTRTSRIRALALEEDGRRNA
                     ETLVKIESDPPRYLNAVYLTVMFVQNGSAILVAILAENDFGPEDGSGPWVGVVSFVFT
                     ILYFVVVEAMAKTFAVLHTDRVALTLAPVVWFLGRVLELPTRFLIGLANVLLPGKGLK
                     QGPFVSEEEIRSMAEVGSEEGSIDEGEKELIHSIFEFGD"
BASE COUNT          457 a          923 c          977 g          416 t
ORIGIN      
        1 caagggcaaa ggggttccgc acctgcagcg ccgcgggcgt ggcgatctct tcgtgacgct
       61 gcacatcgtg acgccccgcg acctctcgaa ggaggagcgc acgctcctcc agcggttcgc
      121 cgagatccgc gacgcgggtg ccggaggcaa cggcgccgcg ccgcgcgcac tccggcgacc
      181 cgagtatcgt tcgtgatcgc tcggccgtga gcgcggaagg agggacaccg ggcatgcaga
      241 ccgacagctc gtgcctgttc tgccgcatcg cggcgcgcga gatcccggcg gacatcgtgc
      301 gcgagtcgga ccggctcgtg gcgttccgcg acaccaaccc ccaggcgtcg acgcacatct
      361 tgctgatccc gaaggagcat gtgcgctcga tcgcggagat cgaggatcag cacgcggacc
      421 tcctggccga tatcgcccaa gcggctacgc agctggccgc cgccgagggg atcgaagagt
      481 cggggtggcg ccttgtgacg aacgtcggcc cggacgccgg ccagagcgtg ttccacctgc
      541 acttccacct cctcggtggg cggcagatgg gctggccgcc gggataaacg cgataggatg
      601 cgagtcgcgg gacccagacg ggtcccgctc tcgacaacag gaggaacggg gtcacccccg
      661 agagcgcgac acgtggcagc cgccaccacc caagtgaaga tcctcgtccc cggccaccag
      721 ccgatggtgg cgctcctggg ccagcaggac gcgttcctga agttgatcga agccgggttc
      781 tcctccgaca tcctcgtccg aggcaacgag atcacgatca ccggccctga gcacgaggcc
      841 gaacagctct cgcatctctt cgaagagctc ctcgcgctgc tcgagaaagg ccaccacctc
      901 tccgactcct ccgtcggcca gacgatcgag ctgatcaagg gcggcgacga gtgggtggag
      961 ggcgaggacg ggacggccag gatgcgcccg agcgaggtgc tgggcgacac gctcctcacc
     1021 gcgcggggga cctcggtcgt gcccaagacg gtcggccaga agcggttcgt cgacgcgatg
     1081 cggcgggcga cggtgacgtt cgcgatcgga cccgccggca cgggcaagac ctacctggcg
     1141 gtcgccaccg cggtacgcgc gctccaggat caccaggtct cgcggctgat cctcacccgt
     1201 cccgcggtgg aggcgggaga gcggctaggg ttcctgccgg ggacgctcta cgagaagatc
     1261 gatccgtaca tgcgcccgct ctacgacgcg ctgttcgaga tgaccggcgc cgagagcctc
     1321 tcgcggctga tggagcgggg cacggtcgag gtcgcgcccc tcgcctacat gcgcggcagg
     1381 accctgaacg acgcgttcat catcctcgac gaggcgcaga acacgacgcc ggagcagatg
     1441 aagatgttcc tcacgaggat cgggttcggg tccaaggcgg tcgtcaacgg cgacgacacg
     1501 caggtggacc tcgccaccgg gcagcgctcg ggtctcctcg tgatcgagga gatcctctcg
     1561 ggcatccgcg gcatctcgtt ctgccggctc ggcgggcggg acgtcgtgcg gcacaagatc
     1621 gtccaggaga tcgtcgaggc ctacgaccgt tacggtcagc agcgcctcgg gatggacgac
     1681 tgatggccga cgacgaccat cccgtcgacc ctggaccggc ggacggtgcc tggccccgcg
     1741 tcctgatctc gaaccgtcag cggcgggaac tcgatgtgga ggggctccgc tcgctcgccc
     1801 gcgacaccct gcgaggcgaa ggggtcgatc gcgccgagct ctcggtgtcc ttcgtggaga
     1861 ccggcgagat cgaggagctc cacgagcggt tcatggacga gccgggaccg acggacgtcc
     1921 tgtcgttccc gctcgtcgag gaggccgaag gacccgctaa ggacggagcc aaggacgggg
     1981 atggaacgcg ccacctcggc gatgtggtcg tcgcgccggc ggaggcggag cgcaacaacc
     2041 cggccgatcc cgatggggag atccgcctgc tgctcgtcca cgggatcctg cacctcctcg
     2101 gctacgacca cgaggacgag gacgcgcgca cccggatgtg ggagcggcag gagcgctaca
     2161 gcggggtgcg cgccccgtga gctcgctctg gctcgtcgcg ctcctgctgg tgctgttcgg
     2221 gaccgttctc gcggccgcgg aggcatcgct cacacggacg tcgcggatcc gcgcgctggc
     2281 cctcgaggaa gacggtcggc gcaacgccga aacgctcgtg aagatcgagt ccgatccacc
     2341 gcgctacctg aacgcggtct acctcacggt gatgttcgtg cagaatggct cggccatcct
     2401 cgtcgcgatc ctcgccgaga acgatttcgg ccccgaggac ggcagcggcc cgtgggtcgg
     2461 tgtcgtctca ttcgtgttca cgatcttgta cttcgtcgtg gtcgaggcga tggcgaagac
     2521 gttcgcggtc ctgcacacgg accgagtggc gctgacgctg gcgccggtcg tgtggttcct
     2581 cggccgcgtc ctcgagctcc cgacacggtt ccttatcggc ctggcgaacg tgctgctccc
     2641 cggtaagggc ctgaagcagg ggccgttcgt ctcggaggag gagatccgct cgatggccga
     2701 agttgggtcg gaggagggct cgatcgacga gggcgagaag gagttgatcc actcgatctt
     2761 cgagttcggc gac
//
LOCUS       DASUVE010000378         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1317135, whole genome shotgun sequence.
ACCESSION   DASUVE010000378 DASUVE010000000
VERSION     DASUVE010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317135"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1867)
                     /locus_tag="VF036_08120"
     CDS             complement(<1..1867)
                     /locus_tag="VF036_08120"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX6265231.1"
                     /translation="MAGPGTEMPPRYDAAAVEPRWAEAWIERGSFHAEVPDDPSEAYC
                     IVIPPPNVTGRLHVGHALGRTLEDVLIRRARMRGQEALWMPGMDHAGIATQVVVEREL
                     WKEGIERRELGRDAFVARVWEWKERYGGEIIGQLKRMGASLDWDRERFTMDEGLVRAV
                     RIAFVRWYEQGLIYRGERLVNWCPTDQTGLSDSEVEHEDVDGELLTFRYPLSEGSGHV
                     DVVTTRVETMLGDTGIAVHPDDDRYRDLVGKTVRHPFDARELPIVADEVVDPAFGTGA
                     VKVTPAHDPTDFDIAQRTGLPSLNVLDAEARINEHAAEEFYGLGRYDARQAVREELEN
                     LGLFVGVEHPYRHSVGHCYRCHSEIEPWLSGLQWFVAVEDLKRPAIEAARDGRITFWP
                     ERWRKAYVSWMEGLRDWNISRQLWWGHRIPAWYCPDEHITVALDDPDDCATCGSAELE
                     QDPDVLDTWFSSQLWPFSTLGWPDDTPELRAFYPNAVLVTGYEILYLWVARMIMSGMS
                     LAGDIPFRDVVIHGLVRDGHGRKMLKSLGNVIDPNEMIDLHGADALRFSLARLATGGQ
                     QDIPLSVDSIKGGRNFANKIWNAARLVLQEVPGGEPELPPEERLTAPQRWLLARHQ"
     gene            1973..2395
                     /locus_tag="VF036_08125"
     CDS             1973..2395
                     /locus_tag="VF036_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6265232.1"
                     /translation="MAKEALIDRLGTVAIFRGLDTKELHRIAEVGKEVHFEPGAVVAQ
                     QDGGAAGFHLIMDGEVSIDVDGHERARLGAGNYFGEMSLLDGLPRSATVKAEEPTTTF
                     ALTSWQFLPLLDEYPSISRALLVELCARLRRVEHDPVS"
BASE COUNT          382 a          854 c          794 g          375 t
ORIGIN      
        1 gctggtgtcg agcgaggagc caccgctggg gcgccgtgag ccgttcctcc ggtgggagct
       61 ccggctcgcc gccggggacc tcctgcagga cgagccgggc ggcgttccag atcttgttcg
      121 cgaagttgcg cccgcccttg atcgaatcga ccgacaaggg gatgtcctgc tggccgccgg
      181 tcgcgagtcg cgcgagggag aagcgcaggg cgtccgctcc gtggagatcg atcatctcgt
      241 tcggatcgat cacgttcccg agcgacttca acatcttgcg gccgtggccg tcgcgcacga
      301 gcccgtggat cacgacgtcg cggaagggga tgtcgccggc gagggacatg ccggacatga
      361 tcatccgcgc gacccagagg tagaggatct cgtagccggt cacgaggacg gcgttcgggt
      421 agaacgcgcg cagctccggc gtgtcgtccg gccagccgag cgtcgagaac ggccacagct
      481 gggacgagaa ccacgtgtcc agcacgtccg ggtcctgctc gagctccgcc gatccgcagg
      541 tggcgcagtc gtcgggatcg tcgagtgcga ccgtgatgtg ctcatccggg cagtaccacg
      601 cggggatccg gtggccccac cagagctgcc gcgagatgtt ccagtcgcgc agaccctcca
      661 tccagctcac gtaggccttc cgccaccgct ccggccagaa ggtgatccgc ccgtcgcggg
      721 ccgcctcgat cgcagggcgc ttcaggtcct cgaccgccac gaaccactgc agcccggaca
      781 gccacggttc gatctcgctg tggcaccggt agcaatgccc cacggagtgc cgatacggat
      841 gctcgacgcc gacgaacaga ccgagattct cgagctcctc gcgtaccgcc tgacgtgcgt
      901 cgtagcgacc cagcccgtag aactcctcgg ccgcatgctc gttgatccga gcttcggcat
      961 cgaggacgtt cagcgagggg aggcccgtgc gctgcgcgat gtcgaagtcc gtgggatcgt
     1021 gcgccggcgt gaccttcacg gcccccgtgc cgaacgccgg atcgaccacc tcgtcggcga
     1081 cgatcggaag ttcgcgtgcg tcgaacgggt gccgcaccgt cttcccgacc aggtcgcggt
     1141 accgatcgtc gtcgggatgc accgcgatcc cggtgtcgcc tagcatcgtc tcgacgcggg
     1201 tggtaacgac gtcgacgtga ccgctcccct cggagagcgg gtaccggaac gtcaggagct
     1261 cgccgtcgac gtcctcgtgc tccacctcgg aatcggacaa tccggtctgg tcggtcggac
     1321 accagttcac gagccgctcg ccacggtaga tcaacccctg ctcgtaccaa cggacgaacg
     1381 cgatccgcac ggcgcgaacg agtccctcgt ccatcgtgaa ccgctcgcga tcccagtcca
     1441 gcgaagcacc catccgcttc agctggccga tgatctctcc gccgtatcgc tccttccact
     1501 cccagacccg cgccacgaac gcatcgcggc cgagctcgcg ccgctcgatg ccctccttcc
     1561 acagctcccg ctcgaccacg acctgggtcg cgatgccggc atgatccatc cccggcatcc
     1621 agagcgcctc ctggccccgc atccgagctc ggcggatcag gacatcctcg agcgtccgtc
     1681 cgagcgcgtg accgacgtgg agccggccgg tgacgttcgg cggcgggatc acgatgcaat
     1741 acgcctccga gggatcgtcc ggcacctcgg cgtggaagga accgcgctcg atccacgcct
     1801 ccgcccatcg cggctcgacc gcggccgcgt cgtagcgcgg cggcatctcc gttccggggc
     1861 cggccatgcg atcgagtgta tcgacccacc tgggagcgcc cgaacgcgaa catctcgccc
     1921 gggagcgcgt cctatactcg cgcgacaccc acccgcccgg aggagggatc tcatggcgaa
     1981 ggaagcgttg atcgatcgtc tgggcacggt cgcgatcttc cgtggcctgg acacgaagga
     2041 gctccaccgg atcgctgagg tcggcaagga ggtccacttc gagccgggcg cggtcgtggc
     2101 acagcaggac ggcggcgccg cggggttcca cctgatcatg gacggcgagg tctcgatcga
     2161 cgtggacggc cacgagcgcg cccggctcgg cgcggggaac tacttcgggg agatgtcgtt
     2221 gctcgacggg ctcccccggt cggcgaccgt caaggcggag gagcccacga ccaccttcgc
     2281 cctgacctcg tggcagttcc tgccgctcct ggacgagtac ccatcgatca gccgggcgct
     2341 cctcgtggag ctctgcgcgc gactccggcg agtggagcac gatcccgtca gctgacgggt
     2401 tcgtc
//
LOCUS       DASUVE010000379         3693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1129065, whole genome shotgun sequence.
ACCESSION   DASUVE010000379 DASUVE010000000
VERSION     DASUVE010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129065"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /gene="mmsA"
                     /locus_tag="VF036_08130"
     CDS             <1..1023
                     /gene="mmsA"
                     /locus_tag="VF036_08130"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating, NAD) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX6265233.1"
                     /translation="LWMLTNAIACGNTFVLKPSEKDPGASLVLAELLRDAGVPDGVFN
                     VVHGDKVAVDAILDDPTIQAGSFVGSTPIAKYIYEHGTKAGKRVQALGGAKNHMIVLP
                     DADLDMAADAAVSAGYGSAGERCMAISAIVAVGGIADDLVEKIAERLPKVKVGPGTDP
                     EAEMGPLVTREHRDKVAGYLDSSAEQGATVVRDGREDSRFGGDGFFLGVSLLDHVTEE
                     MDAYRDEIFGPVLSVVRVDTYDEALKMVNDNPYGNGVAIFTRDGGAVRQFQFDVNVGM
                     VGVNVPIPVPVAYYSFGGWKSSLYGDNHMYGPEGIQFYTRGKVVTARWPDPATSAVDL
                     GFPRTR"
     gene            1023..1481
                     /locus_tag="VF036_08135"
     CDS             1023..1481
                     /locus_tag="VF036_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265234.1"
                     /translation="MPSAEELLQAEEAAWAEFVAEVNRVPEHRRGEEGVVPGWSVNDL
                     VFHNGRWAGVAAEKLKLIRDSGSPGEEDPDEVWRGKNDLWAAESKSMLYQEAMSRALA
                     DREAAREALQALPEVTDEAASWFKEETFDHYQEHTEEVSRFADSLSVPES"
     gene            1519..1989
                     /locus_tag="VF036_08140"
     CDS             1519..1989
                     /locus_tag="VF036_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6265235.1"
                     /translation="MNVSLRPVEDRDLDAIYQQMIDPASIRMAAFTAKDQTDRRAVLN
                     KILRLRADTSVSFRVIDVDGAAAGTIGSFRIDDQPEVTYWVDRKLWGKGVASAALEIL
                     LAETAERPLFARAASDNLGSLRVLEKAGFRRIGINRGFAHGRDEEIEETIMRLD"
     gene            complement(2048..2536)
                     /locus_tag="VF036_08145"
     CDS             complement(2048..2536)
                     /locus_tag="VF036_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265236.1"
                     /translation="MRRARRRRTSTSSSTSSEIALIDPDDEEVTTVFQEAGFMRAGLD
                     VRFFGDSHSPTAPHLFSWFIELDPEEGAMSALDLLEADTMKPCPQSCAAVISAFDVDG
                     IPDARGVRRIATAEDIEAAGTEDEDPSESYWVGFTAGSIVYTLDLGDSRDPYPRSKPR
                     GS"
     gene            complement(2745..3308)
                     /locus_tag="VF036_08150"
     CDS             complement(2745..3308)
                     /locus_tag="VF036_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6265237.1"
                     /translation="MGDVVIDMSMSLDGYIAAPNDSPEQGLGEDGMRLHNWMFDDPTV
                     FERVFGDLTENTGAVIMGRRTYDNSIEAWGGKGPLGEVPCFVVTHRPVEGADPLFTFV
                     TDGIESALAKAQEAAGDKRIGLMGANLDQQFLAAGLVNEIRIHLLDVLLGSGRRLFDE
                     LPKRTELELTGVSQTGGVTHLEYRVAG"
     gene            complement(3366..>3693)
                     /locus_tag="VF036_08155"
     CDS             complement(3366..>3693)
                     /locus_tag="VF036_08155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6265238.1"
                     /translation="TLANAEARHEEMWEWIARNFPDEPFWYLDHIAVDAAHRGSGLGA
                     ALIRLGLDFADRDGVPAFLETGRPGNVAYYERRGFRTISDDDAPGGGPQIWFMRYDPR
                     PSEPGS"
BASE COUNT          644 a         1199 c         1237 g          613 t
ORIGIN      
        1 ctctggatgc tcacgaacgc gatcgcgtgc ggcaacacgt tcgtcctgaa gccgtcggag
       61 aaggatccgg gggcctccct tgtgctcgcg gagctgctgc gggacgccgg cgtccccgac
      121 ggagtcttca acgtcgtcca cggcgacaag gtcgcagtgg acgcgatcct ggacgacccg
      181 acgatccagg cggggtcgtt cgtcggctcg accccgatcg cgaagtacat ctacgagcac
      241 ggcacgaagg ccggcaagcg cgtccaggcg ctcggcggtg cgaagaacca catgatcgtc
      301 ctccccgacg ccgacctcga catggcggcc gacgccgcgg tctcggccgg ctacgggagc
      361 gcgggtgagc gctgcatggc gatctcagcg atcgtggccg tcggtgggat cgccgatgac
      421 ctcgtcgaga agatcgccga gcgcttgccg aaggtgaagg tcgggccggg caccgatccc
      481 gaggcggaga tggggccgct cgtgacgcgc gagcaccgcg acaaggtcgc gggatacctc
      541 gactcgagcg ccgagcaggg ggctacggtc gtccgcgacg gtcgtgagga ctcgcggttc
      601 ggcggcgacg ggttcttcct cggcgtctcg ctcctcgatc acgtcaccga ggagatggat
      661 gcctaccgcg acgagatctt cggcccggtg ctctcggtgg tgcgggtgga cacctacgac
      721 gaggcgctga agatggtcaa cgacaatccg tacgggaacg gcgtcgcgat cttcacgcgt
      781 gacggcggcg cggtccggca gttccagttc gacgtgaacg tcgggatggt cggtgtgaac
      841 gtcccgatcc cggtcccggt cgcctactac tccttcggtg ggtggaagag ttcgctgtac
      901 ggggacaacc acatgtacgg gcccgagggg atccagttct acacgcgcgg caaggtcgtg
      961 acggcgcgat ggcccgatcc cgccacgtcc gcagtcgacc tcggcttccc gaggacacgc
     1021 tgatgccgtc ggccgaggag ctgctgcagg cggaagaggc cgcgtgggcc gaattcgtcg
     1081 cggaggtgaa ccgcgtgccg gagcaccgtc gcggggagga gggagtcgtc ccgggctggt
     1141 ccgtgaacga cctggtgttc cacaacggga ggtgggcggg cgtcgctgcc gagaagctga
     1201 agctgatccg cgactccggg agcccaggcg aagaggatcc cgacgaggtc tggcgaggga
     1261 agaacgatct ctgggcggcc gagtcgaaat cgatgctcta ccaggaggcg atgtcgcgcg
     1321 ccctcgccga tcgcgaggct gcgcgggagg cgttgcaggc actccccgag gtcaccgacg
     1381 aagctgcttc ctggttcaag gaggagacgt tcgaccacta ccaggagcac acggaggagg
     1441 tcagccgctt cgccgacagc ctcagcgtcc cggagagtta accgacgagc ctcaagagag
     1501 ctgaggatgg atgcccagat gaacgtctcg ctgaggcctg tcgaggatcg ggatctcgat
     1561 gcgatctatc agcagatgat tgatccggcg tcgatccgga tggccgcctt cacggccaag
     1621 gatcagaccg atcgtcgcgc ggtcctcaac aagatcttgc gccttcgggc cgacacgagt
     1681 gtctcgttcc gagtcatcga cgttgacgga gcagccgctg gaacgatcgg gtctttccgg
     1741 atcgatgatc agcctgaggt gacctactgg gtggaccgaa agctctgggg gaaaggcgtc
     1801 gcgagcgcag cactcgagat ccttcttgcc gagaccgccg aacgtccgct gttcgcccga
     1861 gcggcctcag acaacctcgg ttcgcttcga gtgctggaga aagccgggtt ccggcgcatc
     1921 gggatcaatc gcggcttcgc acacggtcgc gatgaggaga tcgaggaaac gatcatgcgc
     1981 ctcgactgag ggaggcgaga ctgcgttccg ccccgctagc ctcctgtaag ccgctcgtag
     2041 tacgagctca cgatcccctg ggcttgctcc tcggatacgg atcccgggag tcccccagat
     2101 caagcgtgta gacgatcgaa cctgctgtga accccaccca gtagctttcg gaaggatctt
     2161 catcctcggt cccggcggcc tcgatgtcct cggccgttgc gatccgacgg acgccgcgcg
     2221 catccgggat gccatccacg tcgaaggcgc ttatcaccgc ggcacagctc tgcgggcacg
     2281 gcttcatcgt gtccgcttcg aggaggtcga gtgcgctcat cgccccttcc tcaggatcaa
     2341 gctcgatgaa ccaactgaag agatgtggcg cggtgggcga atgcgaatcc ccgaagaagc
     2401 gtacgtccag acccgccctc atgaaaccgg cctcctggaa gaccgtggtg acctcttcgt
     2461 cgtcgggatc gatcagggca atctctgaag atgtcgatga actcgtcgag gtcctccgtc
     2521 gtctcgccct tctcaacggg atcaggctcc gatcccggtg catccgcggt cgtggggaag
     2581 cgctgcgcga ccggggggat catcagcacc gctgcacgcg acgaacgcga ccgccgtcaa
     2641 aggcacaaga agtggacggc gaagcgcatc gatggaccta tgagatgcag gagctactac
     2701 aagacaggcg ttctcggaga aggccgctgt caccgcgtgg actgctaccc ggcgacccgg
     2761 tactcgaggt gcgtcacacc gcccgtctgg ctgactcccg tcagttctag ctcggtccgc
     2821 ttgggcagct cgtcgaacaa ccgacggccg ctcccgagca ggacatcgag caggtggatc
     2881 ctgatctcgt tcacgagccc cgcggccagg aactgctggt cgaggttcgc gcccatgagc
     2941 ccgatccgct tgtcgccggc ggcctcctgg gccttcgcga gggcactctc gatcccgtcg
     3001 gtgacgaacg tgaacagcgg gtcggcgccc tcgacgggtc tgtgcgtgac cacgaagcac
     3061 gggacctcgc cgaggggacc cttgccaccc catgcctcga tcgagttgtc gtatgtccgg
     3121 cggcccatga tcacggcgcc ggtgttctcg gtcaggtcgc cgaacacgcg ctcgaagacc
     3181 gtcgggtcgt cgaacatcca gttgtgcagc cgcatcccgt cttcaccgag cccctgctcc
     3241 ggggagtcgt tcggcgctgc gatgtagccg tccaaggaca tgctcatgtc gatcaccacg
     3301 tcgcccatgc cgcgtcctcc cgtcgtcgac aacaacagcc gctgtcacga acctatcgcg
     3361 gatgctcagg agcctggctc gctcggtcgg gggtcgtagc gcatgaacca gatctgcggg
     3421 ccgccgccgg gagcgtcgtc gtccgagatc gtgcggaagc cccggcgctc gtagtacgcc
     3481 acgttgccgg gccgtccggt ctcgaggaac gccggcacgc catctcgatc ggcgaagtcc
     3541 agcccaaggc ggatcagcgc cgcccccagg cccgagcccc gatgcgcggc gtctacggcg
     3601 atgtgatcga ggtaccagaa cggctcgtcc gggaagttcc tcgcgatcca ctcccacatc
     3661 tcctcgtggc gagcctccgc gttcgcgagc gtc
//
LOCUS       DASUVE010000380         5189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_187002, whole genome shotgun sequence.
ACCESSION   DASUVE010000380 DASUVE010000000
VERSION     DASUVE010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187002"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1771
                     /gene="fusA"
                     /locus_tag="VF036_08160"
     CDS             <1..1771
                     /gene="fusA"
                     /locus_tag="VF036_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020572746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX6265239.1"
                     /translation="DAVAGVEPQSETVWRQADRYRVPRICFINKMDRTGANFERTVDM
                     IVDRLQATPLILQLPWGTESDFHGVIDLVQMNAHYWEGDMGEEWHDTEIPEEYRAAAE
                     AAHHVLFERLADHDESLMEKFVHEEEPTIEEMHRAIRRATLASLGVPVMCGSAFKNKG
                     VQPLLDAIVAYLPSPAEVLPVEGHLPNDESTHVQRAPEDTEPFSALAFKIMSDAFVGR
                     LTYLRVYSGVLRAGSHVANATKDRKERIGRILQMHANHREDMAAAYTGDIVAVVGLKH
                     ATTGDTICDPDHQVVLESISFPTPVISVAVEPKTKADQDKLGTGLAKLADEDPTFVVK
                     FDDETGQTVISGMGELHLDIIVDRLKREFSVDANVGKPQVAYRETIRREVHKVETRFV
                     RQTGGRGQFAHVVIDLEPTGAGGGYEFLNKIVGGKIPREYIPAVDQGIQEALDNGVLA
                     GYPMVDVRATLMDGTYHEVDSSEMAFKIAGSMALKEAARKADPALLEPVMEFEVTTPE
                     EFMGEVMGDVTARRGRIERIDDAGGQKVIRVLVPLAELFGYATDLRSRTQGRAAHSPM
                     QLHAYAEVPVQVSKEIIARVSGE"
     gene            1822..3006
                     /gene="tuf"
                     /locus_tag="VF036_08165"
     CDS             1822..3006
                     /gene="tuf"
                     /locus_tag="VF036_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX6265240.1"
                     /translation="MGKKKFERTKPHVNIGTMGHIDHGKTTLTAAITKRQAEKGMAEF
                     TPFDEIDKAPEERERGITIAIAHVEYETDNRHYAHVDMPGHADYVKNMITGAAQVDGA
                     ILVVSAADGPMPQTREHVLLARQVGVPSIVVALNKCDMVDDPELLDLVELEVRELLTS
                     YEFPGDDVPVIQISALKALEGDESWMAKVDELLEACDTYIPEPVRDTDKPFLMPIEDV
                     FSITGRGTVVTGRVEQGVLKKMGEVEIVGLRDTQKTTATDLEMFRKLLDEITAGDNVG
                     VLLRGIDKDQVERGQVLAKPGSITPHTEFMSSVYILSKEEGGRHTPFFDGYKPQFYFR
                     TTDVTGVAHLPEGVEMVMPGDNVEMRVELDKPIAMEEGLRFAIREGGRTVGAGRVTKV
                     LK"
     gene            3040..3348
                     /gene="rpsJ"
                     /locus_tag="VF036_08170"
     CDS             3040..3348
                     /gene="rpsJ"
                     /locus_tag="VF036_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX6265241.1"
                     /translation="MAGPKIRIKLRAYDHRMLDVAARDIVEHVTRTGAKVVGPVPLPT
                     ERSIYTVIRSPHKYKDSREHFQMLTHKRLIDIVDATSKTVQELQRLNLSAGVDIEIKL
                     "
     gene            3354..4007
                     /gene="rplC"
                     /locus_tag="VF036_08175"
     CDS             3354..4007
                     /gene="rplC"
                     /locus_tag="VF036_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX6265242.1"
                     /translation="MAETKGILGTKLGMTQIFDDTRAVPVTVIKAGPSVVSQVKTKET
                     DGYDAVQLSFGSVRPRDLARPMKGHFESHDAKPGRHVVELRTDDAGSYEPGQEITVDV
                     FSPGDRVDVVGVSKGHGTTGVMKRHGFHGLRASHGTKRKHRAPGAIGACATPSRVFKD
                     MPMAGQHGSVRVTVLNLEVVQADVERGLLLVKGAVPGPNGGLLMARSAVKAAAKGGE"
     gene            4007..4681
                     /gene="rplD"
                     /locus_tag="VF036_08180"
     CDS             4007..4681
                     /gene="rplD"
                     /locus_tag="VF036_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX6265243.1"
                     /translation="MASIEKRDAAGKKAGSVELPTEIFESMVSVPLMHQVVVAGLAGI
                     RAGTHSTKTRAQVRGGGRKPWRQKGTGRARQGSIRSPQWVGGGVAHGPTSRDHSQKVN
                     KKMRRGALRSALTDALQSGKLAVVDDLDWSEPKTKDAVGVLTALELEGRILLVLPQPT
                     ESGSVEKSFRNLPNVKIAYSGGLGTYDVLLADRILFTGAAIDRLTSPRAGAEAAAPED
                     AEEVAG"
     gene            4678..4974
                     /gene="rplW"
                     /locus_tag="VF036_08185"
     CDS             4678..4974
                     /gene="rplW"
                     /locus_tag="VF036_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX6265244.1"
                     /translation="MNKSPRDVIVRPIVSEKSYAGIEHNTYTFLVDPRANKTEIKEAV
                     QTIWDVRVLSVNTLKRRGKMKRRGYTRGRRPDEKRAVVTLAEGDSIEIFEGGGS"
     gene            4977..>5189
                     /locus_tag="VF036_08190"
     CDS             4977..>5189
                     /locus_tag="VF036_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015889918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX6265245.1"
                     /translation="MAVRKYKPTTPGRRGGSVSTFDEITRSTPEKALLAKGRKKAGRN
                     VHGRITTRHQGGGTKRRYRLIDFKRNK"
BASE COUNT          970 a         1693 c         1817 g          709 t
ORIGIN      
        1 cgacgccgtc gccggcgtgg agccgcagag cgagacggtg tggcgtcagg ccgatcgcta
       61 ccgggtgccc aggatctgct tcatcaacaa gatggaccgg acgggtgcca acttcgagcg
      121 caccgtggac atgatcgtgg atcgcctcca ggcgaccccg ctcatcctcc agctcccctg
      181 gggcaccgag tccgacttcc acggtgtgat cgacctcgtc cagatgaacg cccactactg
      241 ggagggcgac atgggcgagg agtggcacga caccgagatc ccggaggagt accgagcagc
      301 cgcggaggcc gcccaccacg tcctcttcga gcgcctcgcc gatcacgacg agtccctgat
      361 ggagaagttc gtccacgagg aggagcccac gatcgaggag atgcatcgcg cgatccgccg
      421 agcgacgctc gcgagcctcg gcgttccggt gatgtgcgga tccgccttca agaacaaggg
      481 cgtccagccg ctgctcgacg cgatcgtcgc ctacctcccg tcgcccgccg aggtgctccc
      541 ggtggagggc cacctcccca acgacgagtc cacgcatgtc cagcgcgcgc ccgaggacac
      601 cgagccgttc tcggccctgg ccttcaagat catgtcggat gccttcgtcg gccggctgac
      661 gtacctgcgc gtgtactccg gtgtgctccg cgccggctcc cacgtcgcca acgccaccaa
      721 ggaccgcaag gagcggatcg gtcggatcct gcagatgcac gccaaccacc gtgaggacat
      781 ggcggccgcc tacaccggcg acatcgtcgc cgtcgtcggg ttgaagcacg ccaccaccgg
      841 agacacgatc tgcgatcccg accaccaggt cgtcctggag tccatcagtt tccccacgcc
      901 cgtcatctcg gtggccgtgg agccgaagac caaagcggac caggacaagc tcgggaccgg
      961 tctcgccaag ctcgcggacg aggacccgac gttcgtcgtg aagttcgacg acgagacggg
     1021 ccagacggtg atctcgggga tgggcgagct ccacctcgac atcatcgtgg accgcctgaa
     1081 gcgagagttc tccgtcgacg cgaacgtcgg caagccccag gtcgcctacc gcgagacgat
     1141 ccgccgcgag gtgcacaagg tcgagacccg cttcgtccgc cagacgggtg gacgggggca
     1201 gttcgcgcac gtcgtgatcg acctggagcc gaccggtgcc ggtgggggct acgagttcct
     1261 gaacaagatc gtcggcggca agatcccgcg cgagtacatc cccgccgtcg accaggggat
     1321 ccaggaggcc ctcgacaacg gcgtgctggc gggctacccg atggtcgacg tccgggcgac
     1381 cctgatggac ggcacctacc acgaggtcga ctcgagcgag atggcgttca agatcgccgg
     1441 ctcgatggcc ctgaaggagg cggcccgcaa ggccgatccc gcgctgctcg agccggtgat
     1501 ggagttcgag gtgaccacgc cggaggagtt catgggcgag gtcatgggcg acgtgacggc
     1561 gcggcgcggc cggatcgagc gcatcgacga cgcgggaggg cagaaggtga tcagggtgtt
     1621 ggtgccgctc gccgagctgt tcggttacgc caccgacctg cgttcccgca cgcagggccg
     1681 tgcggcgcac agcccgatgc agctccacgc gtacgcggag gtgccggtgc aggtgtccaa
     1741 ggagatcatc gcccgggtct cgggcgagta gggcccccca agtagagctg aagagaagag
     1801 ctggaagagg agagccaaga catgggcaag aagaagttcg agcgcacgaa gccgcacgtg
     1861 aacatcggca cgatgggtca catcgaccac ggcaagacca cactcacggc cgccatcacc
     1921 aagcggcagg ccgagaaggg gatggccgag ttcacaccgt tcgacgagat cgacaaggcg
     1981 cccgaggagc gcgagcgcgg gatcacgatc gcgatcgccc atgtcgagta cgagaccgac
     2041 aatcgccact acgcgcacgt ggacatgccg gggcacgccg actacgtgaa gaacatgatc
     2101 accggcgccg cgcaggtcga cggcgcgatc ctcgtcgtgt cggctgcgga cggcccgatg
     2161 ccgcagaccc gcgagcacgt cctcctggct cgccaggtcg gcgtcccgtc gatcgtcgtc
     2221 gcgctgaaca agtgcgacat ggtcgacgac cccgaactcc tcgacctcgt cgagctcgag
     2281 gtccgggagt tgctgacctc gtacgagttc cctggcgacg acgtcccggt gatccagatc
     2341 tccgcgctga aggcgctgga gggcgacgag tcgtggatgg ccaaggtcga cgagctcctc
     2401 gaggcctgcg acacctacat cccggagccc gtccgagaca cggacaagcc gttcctgatg
     2461 ccgatcgagg acgtcttctc gatcaccggg cgtgggacgg tcgtgaccgg gcgcgtcgag
     2521 cagggcgtcc tgaagaagat gggcgaggtc gagatcgtcg gactgcgtga cacgcagaag
     2581 acgaccgcga cggacctgga gatgttccgg aagctcctcg atgagatcac ggccggcgac
     2641 aacgtcggcg tgctgctccg gggcatcgac aaggaccagg tggagcgtgg tcaggtcctg
     2701 gcgaagccgg ggtcgatcac gccccacacg gagttcatgt cgagcgtgta catcctgtcc
     2761 aaggaggagg gcggccggca cacgccgttc ttcgacggct acaagccgca gttctacttc
     2821 cggaccaccg acgtcaccgg cgtggcacat ctgcccgaag gggtggagat ggtgatgccg
     2881 ggcgacaacg tggagatgcg ggtcgagctc gacaagccga tcgcgatgga ggaggggctc
     2941 cggttcgcga tccgcgaagg cgggcgcacc gtcggggccg gccgggtcac caaggtcctg
     3001 aagtagggac atcgagggaa gaaagggcga ggggacgacg tggcgggacc gaagatccgg
     3061 atcaagctcc gggcgtacga ccaccggatg ctcgacgtcg ccgcccgtga catcgtcgag
     3121 cacgtgacgc ggaccggcgc caaggtggtc gggcccgtcc cgttgcccac cgagcgttcg
     3181 atctatacgg tcatccgctc gccccacaag tacaaggact cgcgcgagca cttccagatg
     3241 ctcacgcaca agcggttgat cgacatcgtg gacgcgacgt cgaagacggt ccaggagctc
     3301 cagcgcctga acctgtcggc cggcgtcgac atcgagatca agttgtagga gcgatggccg
     3361 agacgaaagg gatcttgggc acgaagctgg ggatgaccca gatcttcgac gacacccgcg
     3421 ccgtgccggt cacggtgatc aaggcgggtc cgtcggtcgt gtcccaggtc aagacgaagg
     3481 agaccgacgg ctacgacgcc gtccagctct ccttcggctc cgtccggccg cgcgacctcg
     3541 cgaggccgat gaagggacac ttcgagtcgc acgacgccaa gcccggccgc cacgtcgtcg
     3601 agctgcggac ggacgatgcg ggcagctacg agccgggcca ggagatcacg gtcgacgtgt
     3661 tcagcccggg cgaccgggtg gacgtggtcg gcgtgtcgaa gggtcacggc acgacgggcg
     3721 tgatgaagcg gcatgggttc cacggcctcc gagccagcca cggcacgaag cgcaagcacc
     3781 gtgcccccgg cgcgatcggt gcctgcgcga cgcccagccg ggtcttcaag gacatgccga
     3841 tggccgggca gcacggcagc gtccgcgtca cggtgttgaa cctcgaggtc gtccaggccg
     3901 acgtcgagcg cggcctcctg ctcgtcaagg gagcggtccc tgggccgaac ggtggcctgc
     3961 tgatggcgcg ttccgccgtc aaggcggcgg cgaagggagg cgagtgatgg cctcgatcga
     4021 gaagcgcgac gcggccggga agaaggcggg ctccgtggag ctgccaaccg agatcttcga
     4081 gtcgatggtg agcgtgcccc tgatgcacca agtggtcgta gccggcctcg ccgggatccg
     4141 cgcggggacg cactcgacca agacgcgcgc ccaggtccgc ggcggtggca ggaagccctg
     4201 gcgccagaag ggaacgggcc gcgcccgcca gggatcgatc cggtctccgc agtgggtcgg
     4261 cggcggcgtg gcccacgggc ccacatcccg cgaccactcg cagaaggtca acaagaagat
     4321 gcgccgcggc gcgctccgct ccgccctcac tgacgcgctg cagagcggga agctcgccgt
     4381 cgtcgacgac ctcgactgga gcgagcccaa gacgaaggac gcggtgggcg tgctcacggc
     4441 gctggagctc gagggacgga tcctcctcgt cttgccgcag ccaacggagt cggggagcgt
     4501 cgagaagtcc ttccgcaatc tcccgaacgt gaagatcgcc tacagcgggg gactcgggac
     4561 ctacgacgtc ttgctcgcgg accggatcct gttcacggga gcggcgatcg accggttgac
     4621 ctcgccgcgg gccggcgccg aggcggccgc ccccgaggac gccgaggagg tggcgggatg
     4681 aacaagtcgc ctcgcgacgt gatcgtccgg cccatcgtct cggagaagtc ttacgcgggt
     4741 atcgagcaca acacctacac gttcctggtg gatccccgtg cgaacaagac cgagatcaag
     4801 gaggcggtcc agacgatctg ggacgttcgg gtcctgtccg tcaacacgct gaaacgtcgc
     4861 ggcaagatga agcgccgcgg gtacacgcgc gggcgccgtc ctgacgagaa gcgcgccgtc
     4921 gtcacgctcg ccgagggcga cagcatcgag atcttcgagg gtggggggtc ctaaccgtgg
     4981 ccgttcgcaa gtacaagccg acgacgccgg gccgacgtgg gggcagcgtc tcgacgttcg
     5041 atgagatcac gcggtccacg cccgagaagg cgctgctggc caagggccgg aagaaggcgg
     5101 gacgcaacgt ccacggccgg atcaccaccc ggcatcaggg cggcggcacg aagcggcggt
     5161 accgcctcat cgacttcaag cgcaacaag
//
LOCUS       DASUVE010000381         2708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1506103, whole genome shotgun sequence.
ACCESSION   DASUVE010000381 DASUVE010000000
VERSION     DASUVE010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506103"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VF036_08195"
     CDS             <1..219
                     /locus_tag="VF036_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX6265246.1"
                     /translation="DPIEGDAYARSDLPELPGSLEAAIAAFGQDDTLREALGTGFADY
                     YVTSRVWELKAWRETVTDWERDRYDRSV"
     gene            complement(226..1656)
                     /locus_tag="VF036_08200"
     CDS             complement(226..1656)
                     /locus_tag="VF036_08200"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6265247.1"
                     /translation="MTELLVLGGERVAATEGATTEVVEPSTGKPAWEVAQASAEDARR
                     AVDVAVEAFESGPWRRMSARERGHILTEASFLIRDKAEELATLEARNGGKPIGGARAE
                     IDIVANTYEYWGGAANKIFGETIPIVPPGLDVTLREPVGPCVLISPWNFPAVIANWKV
                     APALACGNTAVLKPASQTPLTALAIADLLMEAGLPEGTLSVLPGPGSTTAAALIADPR
                     VAKIGFTGSTEVGTQVMAAAAANITRVSLELGGKSANVVFDDADLDVAIPKSLWSVFD
                     NAGQDCCARSRQFVQRSIYDEYVERFSKATEAIALGEPLDEATEMGPLISQGQRQTSL
                     DYLAIGQEEGGRLVTGGEVPDGDGYYLRPAVLADVDNSWRVAREEIFGPVVCLLPFDA
                     EEDAIRLANDTPYGLSGSVWTRDIGRAIRVAKAIRTGVISVNSSSSVFTEAPFGGFKQ
                     SGLGREMGMHAAALYTEVKNVYFSEE"
     gene            complement(1703..2542)
                     /gene="uppP"
                     /locus_tag="VF036_08205"
     CDS             complement(1703..2542)
                     /gene="uppP"
                     /locus_tag="VF036_08205"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HEX6265248.1"
                     /translation="MSILQAIVLGLVQGATEFAPVSSSGHLILVPWMFGWGAIGDPDL
                     QKSFDVALHMGTLLGALIYFRNDVARYLRAWFRSIVARRMTTPDERIAWALVIGTIPG
                     AIVGALFEDLIQGELGAPWLIAVMLVSFGIPLVVVDRLARSDRAFDSIGPKDGLLLGT
                     AQALALQPGVSRSGVTMTAARAMGLDRESAARFSFLLSLPIIAGAGAYKAVDLAQSGF
                     QGYAAEFVAGMAASALSGFVVIWGLLRYLRRHDFTVFLAYRVAVAAVVVILIVANVRD
                     ATL"
BASE COUNT          459 a          965 c          900 g          384 t
ORIGIN      
        1 gatccgatcg agggcgacgc ctacgcgcgc agcgatctcc ccgagctccc gggatcgctc
       61 gaagcggcga tcgccgcgtt cggacaggac gacacgctcc gcgaggcgtt gggcaccgga
      121 ttcgccgact actacgtcac gtcacgtgtc tgggagctca aggcctggcg cgagacggtc
      181 acggactggg aacgcgaccg ctacgaccgg tcggtctgaa acgggtcatt cctcgctgaa
      241 gtagacgttc ttcacctcgg tgtagagcgc cgcggcgtgc atccccatct cgcgaccgag
      301 cccactctgc ttgaagccgc cgaacggagc ttcggtgaac acgctgctcg aagagttcac
      361 gctgatcacg ccggtgcgga tcgccttcgc cacgcggatc gcccggccga tgtcgcgcgt
      421 ccacaccgag cccgacagcc cgtagggcgt gtcgttcgcg agcctgatcg cgtcctcctc
      481 cgcatcgaac gggaggaggc agacgacggg cccgaagatc tcctcccgag cgacgcgcca
      541 ggagttgtcg acgtccgcga gcaccgcagg ccggaggtag tacccgtctc cgtcagggac
      601 ctctccaccg gtcacaagac gcccaccctc ctcctgcccg atcgcgaggt agtcgagcga
      661 ggtctgtcgc tggccctggc tgatcagcgg ccccatctcg gtcgcttcgt cgaggggctc
      721 cccgagcgcg atcgcctcgg tcgccttcga gaaccgctcg acgtactcgt cgtagatcga
      781 gcgctgcacg aactgccgcg agcgcgcgca gcagtcctgg ccggcgttgt cgaagactga
      841 ccagagcgac ttcgggatcg cgacgtcgag gtcggcgtcg tcgaacacga cgttcgcgga
      901 cttcccgccg agctcgaggg acacccgcgt gatgttggcg gccgccgccg ccatcacctg
      961 ggtgcccacc tccgtggagc ccgtgaaccc gatcttcgcg acgcgagggt cggcgatcaa
     1021 cgcggcggcc gttgtggacc ccgggccggg gagcacgctc agcgttccct cggggagacc
     1081 ggcttccatc aggaggtcgg cgatcgcgag ggcggtgagc ggggtctgcg acgcgggctt
     1141 cagaacggcc gtgttcccgc acgcgagcgc gggcgcgacc ttccagttcg cgatcacggc
     1201 cgggaagttc cacgggctga tcagtacgca gggacccacc ggctcccgca gggtcacgtc
     1261 gagccccggc gggacgatcg ggatcgtctc cccgaagatc ttgttggccg cgccgcccca
     1321 gtactcgtag gtgttggcga cgatgtcgat ctcggcgcgc gccccgccga tcggcttccc
     1381 tccgttgcgc gcctcgagcg tggcgagctc ttcggccttg tcacggatca ggaacgaggc
     1441 ctccgtgagg atatgtcccc gctcacgagc gctcatgcga cgccacggcc ccgactcgaa
     1501 cgcctccacg gccacatcga cggcccggcg ggcatcctca gcggatgcct gcgcgacctc
     1561 ccacgcaggc ttccccgtcg agggctccac cacctcggtg gtcgcgccct ccgttgccgc
     1621 cacccgctct ccaccgagca ccaagagttc ggtcatcgtc gcctccgtcc acgcacgtcc
     1681 gcgatcaggt gcgaagacta gttcagagcg tggcgtcgcg gacgttcgcg acgatcagga
     1741 tgacgacgac ggcagccacg gccacccggt acgcgaggaa gaccgtgaag tcgtgtcgcc
     1801 gcagatagcg caggagtccc cagatcacga cgaagccgct gagcgccgag gcggccatcc
     1861 ccgcgacgaa ctccgccgcg taaccctgga acccggactg ggcgagatcg accgccttgt
     1921 acgcgcccgc gcccgcgatg atggggagcg acaagaggaa cgagaagcgc gccgccgact
     1981 cccggtcgag gcccatcgcc cgagcagccg tcatcgtgac cccggagcgg gacacgcccg
     2041 gctggagagc gagcgcctgc gccgtgccga gcagcaggcc gtccttcggt ccgatcgagt
     2101 cgaacgcgcg atcgctccgg gcgagccgat cgacgacgac gagcgggatc ccgaacgaca
     2161 ccagcatcac cgcgatcaac cacggagcgc cgagctcccc ttggatcaga tcctcgaaca
     2221 gcgctccgac gatcgccccg gggatcgtgc cgatcaccaa agcccacgcg atccgctcgt
     2281 cgggtgtggt catccgccgg gcgacgatcg aacggaacca ggcgcgcagg tacctcgcga
     2341 cgtcgttccg gaaatagatc aacgcaccga ggagcgtgcc catgtgcagc gcgacgtcga
     2401 aggacttctg gaggtcgggg tcgccgatcg ctccccatcc gaacatccac ggcacgagga
     2461 tcaggtgtcc cgagctcgag accggtgcga actccgtcgc cccctgcacg aggccgagga
     2521 cgatcgcttg aaggatgctc acacggtcct tccgtcggaa aggtccgcct gatgctagcg
     2581 gccggccgtc accggaacgg tgaaggccga gccgggaacg agatcgtcgg gaccccggac
     2641 ggcgacggag agcgtggcga acgccgacgc gaacgcgagg gcctccgcgt ccgtgggcga
     2701 gacgagca
//
LOCUS       DASUVE010000382         4643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_1506251, whole genome shotgun sequence.
ACCESSION   DASUVE010000382 DASUVE010000000
VERSION     DASUVE010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506251"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VF036_08210"
     CDS             <1..826
                     /locus_tag="VF036_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265249.1"
                     /translation="AGENDQVVLTGQLLIEADRTVDTAVIFNGRATVEGTVREDLFVM
                     NGDTEISGTVNGDVGVFNGDVAVLSGAEIGGDLVTQSTPQVEDGATIRGSQTNIATKF
                     DYDMGAFAGRFVWWLGYTVSVLILGLLLLAFAPHLDEAVVETIRARLGSSIGWGVALF
                     FLLPIAAILLLVTVVGIPLGLFVMLALALIYTIGYAVATHGVGRLVMRSSSRNVAFLI
                     GLVILRALALIPVVGGLLWLLASVWGLGLLAVAIRAGRTPRAVTTAGAPPPMPVAS"
     gene            910..1359
                     /locus_tag="VF036_08215"
     CDS             910..1359
                     /locus_tag="VF036_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265250.1"
                     /translation="MDEGGHASAVTPELEPDAQHVSRRFVGLLKARDVRLTGSAAGFV
                     AAGGNLSVLNGGCGPVLTGGSITIRNGGCGPVIAQGDASIENGGTQAILAAGSAKIGR
                     KAFVGLVASPKVTIEQGGRVLLSSPMSLAVGVGIGSVVVLLSRLMRR"
     gene            1679..1840
                     /locus_tag="VF036_08220"
     CDS             1679..1840
                     /locus_tag="VF036_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6265251.1"
                     /translation="MPTGTVTFLFSDIEGSTRLVQTLGDDYPELLDQPSVCCEGLSQK
                     VEVSSAALI"
     gene            1917..2959
                     /locus_tag="VF036_08225"
                     /pseudo
     CDS             1917..2959
                     /locus_tag="VF036_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
     gene            3011..3361
                     /locus_tag="VF036_08230"
     CDS             3011..3361
                     /locus_tag="VF036_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX6265252.1"
                     /translation="MQSLWGATSIYVTENGCAAEDAPANDGRVYDSDRVMFLRGCLGR
                     LQRATSEGMPVDGYFLWSAQDNFEWRDGFGNRFGLIYVDFDTLERIPKLSAEWFREAA
                     LRNAVVWRTDRASE"
     gene            3354..4025
                     /locus_tag="VF036_08235"
     CDS             3354..4025
                     /locus_tag="VF036_08235"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX6265253.1"
                     /translation="MSEPTENTDEHRRFARRHDPTRVMALSDGVFAIVITLLVLEVHV
                     PDLSGGRRLADALEEIRPSFVAFLISFLVIAIAWTGHRDLFSFIRLTDRNLVWLNLLY
                     MLPLSLLPFGAALIARHPEEAVALRIYGLLLVAIALTRLLVWLYATNRPHLLFDPIDR
                     PSRRLGVLLAALPAALYVVAILIAERLPTASLWIYAGAPILYFMSLFISRSQAPPGAA
                     ERDFT"
     gene            complement(4045..>4643)
                     /locus_tag="VF036_08240"
     CDS             complement(4045..>4643)
                     /locus_tag="VF036_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6265254.1"
                     /translation="AAAQELTLFVFVTSLSAASMLVESRRLGVSRRMIASPTAIATVL
                     TGEALGRFAIALLQGLLIVLGSTALFGIDWGNLGTVVLSVALLSLAATGAAMLFGALM
                     RSEQQAGAVGVFVGLALAALGGSMVPLEIFPPVMERIAHLTPHAWALGAMDDAITRGA
                     TPAEAWRDLTVLAIYGVVLFAAAVPLFRRSLTSTPAAA"
BASE COUNT          757 a         1507 c         1593 g          786 t
ORIGIN      
        1 cgctggcgag aacgaccagg tcgtcctcac cggacaactg ctgatcgagg cggaccggac
       61 cgtggacaca gccgtgatct tcaacgggcg cgccacggtc gaggggaccg tccgggaaga
      121 tctgttcgtg atgaacggcg acaccgagat ctcgggcacg gtgaacggtg acgtgggggt
      181 gttcaacggc gacgtcgcgg tgctctccgg cgccgagatc ggcggcgatc tcgtcaccca
      241 gagcactccg caggttgagg acggcgcgac gatccgtggc tcgcagacca atatcgcgac
      301 gaagttcgac tacgacatgg gagctttcgc cggccgtttc gtgtggtggc tcggctacac
      361 ggtctcggta ctgatcctgg gtctcctgct gctggccttc gcgccgcacc tggacgaggc
      421 cgtcgtcgag acgatccggg cccggttggg ctcgagcatc gggtggggcg tcgccctctt
      481 cttcctgctg ccgatcgccg cgatcctcct gctcgtgacg gtcgtgggga tcccactggg
      541 actcttcgtg atgctcgcgc tggctctgat ctacacgatc gggtacgccg tggcgaccca
      601 cggggtcggg cgtttggtga tgcgatcctc ctcgaggaac gtcgcattcc tcatcggcct
      661 ggtgatcctt cgggcactcg cgctgatccc tgtcgtcggg ggactcctct ggctgcttgc
      721 gagcgtgtgg ggactcgggc tgctcgcggt cgcgatccgc gctggacgca cgccgcgagc
      781 ggtcacgacc gccggcgcgc caccgccgat gcccgtagcc agctgatccc cgctgcgggt
      841 ccagccgcac gttgcgatcc gacggaggga gcgcggctgg agagcggcga cgaggaacgg
      901 gaggttgaca tggacgaagg agggcacgcc tctgccgtca cgcccgagtt ggagcccgat
      961 gcccagcatg tctcgagaag gttcgtcggg ctcctgaagg cgcgcgacgt gcggctgacc
     1021 ggttccgccg caggattcgt cgcagccgga ggcaacctgt cggtcctgaa tggaggctgc
     1081 ggtccggtcc tgacgggagg gagtatcacg atccggaacg gcggttgcgg tccagtgatc
     1141 gcgcagggag atgcgtcgat cgagaacggt ggcacgcaag ccatcctcgc ggcaggaagc
     1201 gccaagatcg gccggaaagc cttcgtcggc ctcgtcgcgt cgccgaaggt cacgatcgag
     1261 caaggtgggc gagttctgct gagttcgccg atgtctctcg ccgtgggcgt cggcatcggg
     1321 tccgtggtcg tgttgctctc tcgtctgatg cgaaggtagt cgcggaccga gggtcgtagc
     1381 cgcggccgcg gagagctcgc tccaactcgt cgaggtgctc acttcggtgc tcgccgcggg
     1441 ctggatagat gggctgtccg ggtcgagcgg agccatccgg tcgtggggca actcggcacg
     1501 cgtgcatccg tctcctccgc gatccgtatc gcgagttcgg gtgccgccct ccgtgcgact
     1561 gcggtctccc gatcgggcca atagctcccg gcgccggcgc cgcctcgggg cgccgagggc
     1621 gatcgcgtgc cacgcatgat ggttcgaagc gaggtgaccg gtgagggact cagtcgagat
     1681 gccgacggga acggtcacct tcctgttcag tgacatcgag ggaagcaccc gactggtcca
     1741 gacgctcggc gatgactatc cggagctcct cgaccagccc agcgtctgct gcgaggggct
     1801 ttcgcagaag gtggaggtgt cgtcggcggc actgatttga gggccgaagg tttacaggcg
     1861 taccctcccg ataccgcgcc cgctgcttcg gcggcaggag aacaggggtg ttcggaatgg
     1921 gatcgagcga cggcggccac gcgactcgga ccgttcccgc ccgcggattc ccggacggtt
     1981 tccactgggg tgtagcgacg tcggcgtacc agatcgaggg cgcatgggag gaggacggca
     2041 agggcgtatc gatctgggac acattcgccc atacgcccgg caaggtcgcc aacggcgaca
     2101 gcggcgatgt cgccaacgac cactaccacc ggtacgagga ggatgtggcg ctgatgaagg
     2161 acatcggggc caccgcctac cgtttctcga tctcctggcc tcggatcttc ctcgacgggg
     2221 ccggcgtgcc gaaccccaaa ggcctcgact tctacaaccg cttggtcgac gagttgctga
     2281 acgtggggat cgagccattc gccaccctct tccactggga ccttcctctg acgctgcagg
     2341 accgccacgg cggctggcag tcggccgaga cgtccaggcc ttcgccgagt acgccgggta
     2401 catcgccgag cagctggacg atcgcatcaa gcacatcttc accctcaatg aatcccgctc
     2461 gttcaccgac tacgggcacc agggcatgga ggccgacatc ggtggcggca agacccatcg
     2521 ggtgctcctc gcgccggcgc tccagctctc ggatggcgag ctgaaccagg tgcggcacca
     2581 tgcggtgctc gcacacggcc tggcggtcca ggcgatccgc gggcgcgggg cgagtgacat
     2641 caaggtcggt ttcgccgaga acatggaggt cgcggtgccg acagtggaca ccgccgccca
     2701 cgtcaaggct gcggaagcct gcacgcgcga gaccaacgcg ccgttcgcga ccgtgatgct
     2761 ggagggcatc tacacggata cctatctcga gaggccgggg ccgacgcgcc acgattcacc
     2821 gatgaggaac tccggaccat cgcagcgcca ctcgacttcg tcgggatcaa cgtctatcgg
     2881 ccgatggcgt acgtggagcc gtccgatgac ccgccggggt accacgccgt cccgatcaac
     2941 tcctcccatc cgaagatgaa gtcgggctgg cacatcctcg gcggaggttc tgtattgggg
     3001 accgcgccac gtgcagtcat tgtggggagc gacctcgatc tatgtcaccg agaacgggtg
     3061 tgcggccgag gacgcgcccg ccaacgatgg ccgcgtctac gactcggatc gggtcatgtt
     3121 cctgcgcggc tgcctgggga ggctgcagcg cgccacgtcc gaaggcatgc ctgtcgacgg
     3181 ctacttcctc tggagtgccc aggacaactt cgagtggagg gacggcttcg gcaaccggtt
     3241 cgggctgatc tacgtcgact tcgacacact cgagcgcatc cccaagctga gtgccgaatg
     3301 gttccgggag gcagcgctgc gcaacgccgt cgtgtggagg accgatcgag ccagtgagtg
     3361 agccgacaga gaacaccgac gagcaccggc gcttcgcgcg gaggcacgat ccaaccaggg
     3421 tgatggcgct ctcggatggc gtgttcgcga tcgtgatcac gctgctggtg ctggaggtcc
     3481 atgttccaga tctgtcgggg ggacgacgtc tcgcggacgc cctcgaggag atccggccgt
     3541 cgttcgtcgc cttcttgatc agcttcctcg tgatcgcgat cgcgtggacc gggcaccgag
     3601 atctcttcag cttcatccga ctcaccgatc gcaacctcgt gtggctgaac ctgctctaca
     3661 tgctcccgtt gtcgttgttg cccttcggtg ccgctctgat cgcccgccac ccagaggagg
     3721 cggtcgcctt gcggatctac ggcctcctgc tggtggcgat cgcgctcact cgcctgctcg
     3781 tgtggctgta cgcgacgaac cggccacacc tcctgttcga cccgatcgat cgtccttcga
     3841 ggaggctcgg ggtactgctc gccgccctgc cggcggcgct gtacgtcgtg gcgatcctga
     3901 tcgccgaacg gttgccgacg gcgagcctgt ggatctacgc gggcgcaccg atcctgtact
     3961 tcatgagcct cttcatctcg cggtcgcagg cgccgcccgg cgctgccgag cgagacttca
     4021 cgtgatacag ctctcgagca gggatcaggc ggctgctggc gtgctggtga gcgagcgccg
     4081 gaacagcggt accgccgccg cgaacagcac cacaccgtag atcgcgagga cggtgagatc
     4141 gcgccacgcc tcggcagggg tggctccgcg cgtgatcgcg tcgtccatcg cgcccaatgc
     4201 ccaggcgtgg ggcgtcaggt gcgcgatccg ctccatgaca ggagggaaga tctcgagcgg
     4261 aaccatcgag ccaccgagcg cggcgagcgc cagtcccacg aacactccga ccgcccccgc
     4321 ctgctgttcc gaccgcatca aggccccgaa gagcatcgcc gctcccgtcg ccgcgagtga
     4381 gagcaatgca acgctgagga cgacggtgcc caggttcccc caatcgatcc cgaagagcgc
     4441 cgtcgatccg agcacgatca ggagaccttg aaggagcgcg atcgcgaagc gaccgagcgc
     4501 ttcccccgtg agcacggtcg cgatcgctgt gggggaggcg atcatccgac gggagacgcc
     4561 gagtcggcgc gattcgacga gcatcgacgc agccgacagg gaggtcacga acacgaagag
     4621 cgtcagttcc tgggcggctg ccg
//
LOCUS       DASUVE010000383         3281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1807
            k141_188100, whole genome shotgun sequence.
ACCESSION   DASUVE010000383 DASUVE010000000
VERSION     DASUVE010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961140
            Sequence Read Archive: SRR25158389
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 17:31:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,651
            CDSs (total)                      :: 1,632
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 19
            tRNAs                             :: 17
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 0 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188100"
                     /isolate="SMAG_U1807"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.54694444 N 117.0319444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="VF036_08245"
     CDS             <1..1331
                     /locus_tag="VF036_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019872149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6265255.1"
                     /translation="DVDGRVIAPGDPDYDDARAMFYGGIDRRPAAIVRVASDADVVRV
                     IAEAQNTGLELAIRSGGHSLVGHSVTEGGIVLDLSEMQALEIDAGSRTAWAESGVTAG
                     AYSEAAAEHGLATGFGDSPSVGVGGITLGGGVGFLVRKYGLTIDALMAADVVTATGEV
                     LRVDEESHPDLFWAIRGGGGNFGVATRFRFRLHEVPAIMGGILILPATVETIAGWAAL
                     SDAAPEELSTIATVMKAPPMPFLPEAAHGKVVILGMIVHVGGGETGERAVAPFRVLAE
                     PLADMLKEMPYPGIYMEEDESFHPIASDRTMFLDRVDADVAATILDRLESSDAMMAAA
                     QFRVLGGAMARVPTDATAFAHRSSKIMVNVAALFENVEDRPRYEGWVQDFADEIRQDD
                     MGAYVNFLEDEGPERVRAAYPGATWDRLREIKGRYDPTNLFRLNQNIPPA"
     gene            complement(1373..2269)
                     /locus_tag="VF036_08250"
     CDS             complement(1373..2269)
                     /locus_tag="VF036_08250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6265256.1"
                     /translation="MTPIRLTGPRVTLRSTTPDDAATLTPIRDEPEVFRWWGHLEPGE
                     MDEFVADEHSLAIEVDGAVVGAVQYGEEEDPMYRNANIDVYLTASRHGEGLGSEAVLV
                     LARYLIQERGHHRLTIDPAADNAPAIRAYEKVGFRPVGVLRSYEQGPDGTWHDGLLMD
                     LLGGELIEEAAPHASPRYESIFPIISTRDLPRLLGFYGGLLGGEIRYRFPSEGPPVFV
                     TVRIGSDEIGIAASEDASEDAAQRVAMWIYVDDCDLAFARFRDAGVTIVEEPADQPWG
                     ERIARLLDPDGNEVIVGSRSSP"
     gene            complement(2266..>3281)
                     /locus_tag="VF036_08255"
     CDS             complement(2266..>3281)
                     /locus_tag="VF036_08255"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEX6265257.1"
                     /translation="DAAAQAHGLVCPVGVVGHTGVAGLTLGGGMGRLQRHLGFSIDNL
                     RSVELVTADDRVLTVSEDEEPDLFWGIRGAGPNFGVVTSFEFDLHELGPVVTHGFVDY
                     PAERGHEIAGRVREYLETAPDEVFVSFGVATGTEDDGADMIGRPVPYVGATYSGPVDA
                     AEEVLRPLRDLGPVVDTFRQVPYLELQTANDEEMVWGKRFYMKGGFLVELSDDFVEAA
                     LANVSTSHGAITLWAQGGAISRVPEEAMAFTGRDAAFWLGVEADWDDAAEDDAMIEWG
                     RTTMAALEPFTAAGHYVNDMVETGRDVVRSVYGDVKYERLVGLKRAFDPDNVFRLNQN
                     IEP"
BASE COUNT          511 a         1163 c         1117 g          490 t
ORIGIN      
        1 aggatgtcga cggacgcgtg atcgcccccg gcgatcccga ctacgacgac gcccgggcga
       61 tgttctacgg cgggatcgac cgtcggccgg cggcgatcgt tcgcgtggcg agcgacgcgg
      121 acgtcgtccg cgtgatcgcc gaggcccaga acacaggcct cgagctggcg atccggagcg
      181 gcggccacag cctggtcggg cactccgtca ccgagggcgg gatcgtgctc gatctctcgg
      241 agatgcaggc gctggagatc gacgccggga gccgcaccgc gtgggcggag tccggcgtca
      301 ccgccggcgc gtactcggag gcggccgccg agcacggtct cgccaccggg ttcggcgatt
      361 ctccctccgt gggggtcgga ggcatcacgc tcggcggcgg cgtcgggttc ctcgtgcgga
      421 agtacggcct cacgatcgac gccctcatgg cggccgacgt cgtgaccgcg accggcgagg
      481 tcctgcgcgt ggatgaggag tcgcacccgg acctgttctg ggcgatacgc ggcgggggcg
      541 gcaacttcgg ggtcgcgacc cggttccggt tccgcctcca cgaggtgccc gcgatcatgg
      601 gaggcatcct gatcctgccg gcgacggtgg agaccatcgc gggatgggct gcgctgtccg
      661 atgcggcgcc cgaggagctc tcgacgatcg ccaccgtgat gaaggcgccg ccgatgccgt
      721 tcctccccga agcggcccac ggcaaggtcg tgatcctcgg catgatcgtg cacgtcggtg
      781 gcggtgagac cggcgagcgg gctgtggcgc cgttccgcgt gctcgcggag ccgctcgccg
      841 acatgctgaa ggagatgccg taccccggga tctacatgga ggaagacgag agcttccacc
      901 cgatcgcgtc ggaccggacg atgttcctcg atcgggtgga cgcggacgtc gccgcaacga
      961 tcctggaccg gctcgagtcg tcggacgcga tgatggcagc cgctcaattc agggtgctcg
     1021 gcggcgcgat ggcgagggtg ccgacggacg cgacagcctt cgcgcaccgg tcgagcaaga
     1081 tcatggtgaa cgtcgccgca ctcttcgaga acgtcgaaga ccgcccgcgg tacgaggggt
     1141 gggtccagga cttcgccgat gagatccggc aggacgacat gggtgcgtac gtgaacttcc
     1201 tcgaggacga gggccccgag cgcgtccgcg ccgcgtaccc gggcgcgacc tgggatcggc
     1261 tgcgcgagat caagggtcgg tacgacccga cgaacctgtt ccggctgaac cagaacatcc
     1321 cgccggcgtg agaggtcggg gcccggcgcc tccggagccg cgcccctcca tgtcaaggcg
     1381 aggaacgaga gccgacgatc acctcgttcc cgtccggatc caggagccga gcgatccgct
     1441 ctccccacgg ctgatccgcg ggctcttcga cgatcgtgac gccggcgtcg cggaagcgtg
     1501 caaaggccag gtcgcagtcg tccacgtaga tccacatcgc cacgcgctgc gcggcgtcct
     1561 ccgatgcatc ctccgacgcc gcgatcccga tctcgtcgga tccgatccga accgtcacga
     1621 agaccggcgg cccctcggac gggaatcgat accggatctc gccgccgagg aggcctccgt
     1681 agaagcccaa cagccgcggg aggtcgcgcg tcgagatgat cgggaagatc gactcgtacc
     1741 gcggtgacgc gtgcggcgcg gcctcctcga tcagctcccc tccgagcaga tccatcagca
     1801 acccgtcgtg ccacgtgccg tccggtccct gttcgtacga gcgcagcacg ccgaccggtc
     1861 ggaacccgac cttctcgtag gcgcggatcg cgggcgcgtt gtcggccgcc ggatcgatcg
     1921 tgagccgatg gtgaccgcgc tcctggatca ggtaccgggc gagcacgagg accgcctccg
     1981 acccgagacc ttccccgtgc cgcgacgccg tgaggtacac gtcgatgttc gcgttccgat
     2041 acatcgggtc ctcttcctcg ccgtactgga cggcgccgac gaccgcgccg tcgacctcga
     2101 tcgcgagcga gtgctcgtcg gccacgaact cgtccatctc cccaggctcg agatgacccc
     2161 accagcggaa gacctcgggc tcgtcgcgga tcggggtgag ggtcgccgca tcgtccggcg
     2221 tcgtgctgcg gagcgtgacc cgcggacccg tgagcctgat cggcgtcacg gctcgatgtt
     2281 ctggttgagc cggaacacgt tgtcggggtc gaacgctcgc ttcaacccga cgagccgttc
     2341 gtacttcacg tccccgtaga cgctccggac gacgtcccga cctgtctcga ccatgtcgtt
     2401 cacgtaatga ccggcagccg tgaacggctc gagcgccgcc atcgtcgtgc ggccccactc
     2461 gatcatcgcg tcgtcctccg ctgcgtcgtc ccagtcggcc tcgaccccga gccagaacgc
     2521 ggcatcgcgg cccgtgaacg ccatcgcctc ctcggggaca cgcgagatcg cgccgccctg
     2581 ggcccagagc gtgatcgcgc cgtgcgacgt cgaaacgttc gcgagcgcgg cctcgacgaa
     2641 atcatcggac agttcgacca ggaatccacc cttcatgtag aaccgcttgc cccagaccat
     2701 ctcctcgtcg ttggccgtct gcagctccag atagggcacc tgccggaacg tgtcgacgac
     2761 gggcccgagg tctcggaggg gtcgcagcac ctcctcggca gcatcgaccg gtcccgagta
     2821 ggtggcgccg acgtagggga ccggccgtcc gatcatgtcg gcgccgtcgt cctccgtccc
     2881 cgtcgccacc ccgaacgaca cgaagacctc gtcaggcgcc gtctcgaggt actcgcggac
     2941 gcgtcccgcg atctcgtgcc cccgctcggc ggggtagtcg acgaacccgt gcgtgaccac
     3001 ggggccgagc tcgtggaggt cgaactcgaa cgacgtgacg accccgaagt tcggccccgc
     3061 tccgcggatc ccccagaaga ggtcgggctc ctcgtcctca ctcacggtga gcacgcggtc
     3121 gtccgccgtg acgagctcca cggagcgcag gttgtcgatc gagaagccca agtgacgctg
     3181 gagcctcccc atcccgccac cgagcgtcag cccggcgaca cccgtgtgcc cgaccacccc
     3241 gacggggcag acgagtccgt gggcctgcgc cgccgcgtcg a
//
